Tpp2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tpp2
Entrez Gene ID	81815
Full Name	tripeptidyl peptidase 2
Synonyms	TPP-2
General protein information	Preferred Names tripeptidyl peptidase 2 Names tripeptidyl-peptidase 2 TPP-II tripeptidyl aminopeptidase tripeptidyl-peptidase II
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 9 Map Location: 9q22
Summary	extralysosomal enzyme involved in degrading peptides generated by the proteasome [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006244852.3	XP_006244914.2	tripeptidyl-peptidase 2 isoform X2
XM_006244853.3	XP_006244915.2	tripeptidyl-peptidase 2 isoform X3
XM_006244870.3	XP_006244932.1	tripeptidyl-peptidase 2 isoform X1
NM_031137.1	NP_112399.1	tripeptidyl-peptidase 2

Pathways from BioSystems

Homologies

	TPP2	XP_002800874.1
Mus musculus (house mouse)	Tpp2	NP_033444.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004870	XP_314316.4
Oryza sativa (rice)	Os02g0664300	NP_001047661.1
Xenopus tropicalis (tropical clawed frog)	tpp2	XP_002933059.1
Bos taurus (cattle)	TPP2	NP_001092504.1
Danio rerio (zebrafish)	tpp2	XP_005168068.1
Schizosaccharomyces pombe (fission yeast)	tpp2	NP_594951.1
Arabidopsis thaliana (thale cress)	TPP2	NP_193817.2
Pan troglodytes (chimpanzee)	TPP2	XP_001151602.1
Rattus norvegicus (Norway rat)	Tpp2	NP_112399.1
Caenorhabditis elegans (roundworm)	F21H12.6	NP_495221.1
Homo sapiens (human)	TPP2	NP_003282.2
Canis lupus familiaris (dog)	TPP2	XP_005634119.1
Gallus gallus (chicken)	TPP2	XP_004938615.1
Drosophila melanogaster (fruit fly)	TppII	NP_725252.1

Gene Ontology
Bibliography

Related articles in PubMed

[The changes in the activity of tripeptidyl peptidase II in experimental atherosclerosis and hypertension].
Pashevin DO, Honcharov SV, Tumanovs'ka LV, Dosenko VIe, Mo?benko OO
Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994)60(3)27-31(2014)

The structure of human tripeptidyl peptidase II as determined by a hybrid approach.
Sch?negge AM, Villa E, F?rster F, Hegerl R, Peters J, Baumeister W, Rockel B
Structure (London, England : 1993)20(4)593-603(2012 Apr)

Tripeptidyl-peptidase II expression and activity are increased in skeletal muscle during sepsis.
Wray CJ, Tomkinson B, Robb BW, Hasselgren PO
Biochemical and biophysical research communications296(1)41-7(2002 Aug)

Two new proteases in the MHC class I processing pathway.
Stoltze L, Schirle M, Schwarz G, Schr?ter C, Thompson MW, Hersh LB, Kalbacher H, Stevanovic S, Rammensee HG, Schild H
Nature immunology1(5)413-8(2000 Nov)

Interferon-gamma can stimulate post-proteasomal trimming of the N terminus of an antigenic peptide by inducing leucine aminopeptidase.
Beninga J, Rock KL, Goldberg AL
The Journal of biological chemistry273(30)18734-42(1998 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Changes in the activity of TPP II play an important role in the pathogenesis of blood vessels wall in atherosclerosis and arterial hypertension.
Title: [The changes in the activity of tripeptidyl peptidase II in experimental atherosclerosis and hypertension].

increase of expression and activity in skeletal muscle during sepsis
Title: Tripeptidyl-peptidase II expression and activity are increased in skeletal muscle during sepsis.

The following Tpp2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tpp2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa32176	XM_006244870.3 Latest version!	Rattus norvegicus tripeptidyl peptidase 2 (Tpp2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa62870	XM_006244852.3 Latest version!	Rattus norvegicus tripeptidyl peptidase 2 (Tpp2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ORa62871	XM_006244853.3 Latest version!	Rattus norvegicus tripeptidyl peptidase 2 (Tpp2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa07716	NM_031137.1 Latest version!	Rattus norvegicus tripeptidyl peptidase 2 (Tpp2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$916.30 $1309.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tpp2 ( XM_006244870.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006244932.1 Rattus norvegicus tripeptidyl peptidase 2 (Tpp2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa32176
Clone ID Related Accession (Same CDS sequence)	XM_006244870.3
Accession Version	XM_006244870.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2421bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	tripeptidyl-peptidase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006244870.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGATCC CACGCCTCAG GCAGACCGCC TATTCCTCTC GCGACGTCTC TTGGAGAGTG 
ACAGCTCCGA ATGCAAGTCC CGGTTTCTTC GCTCCCCACA GACCCTTTAA GAACCTCCCT 
CCAGTGCGTT GTCATTGGCA CCAGCGCAAA CTCCACCCCC GCTCTGCAGC CTGGGTAACA 
AACATTCCTG TGTCTTGCCT CGTTGCGGCG CCGTTAGCTT ACGTTGAAGT ATATGATTGG 
CTAGACATAT CTGACCGCAT GCGATTGGGT ACTGGTGGCC AGGCCCCGCC CCTTCCCCTC 
TTCCGTCCCG CGGTCGCCGG GTGTCCTCGC GCCCCTCGTC CGCGCGCTGC CTGGCAGTTT 
ACCTCTTCCA CGTCCGTCCT CCAGCTTGCG TCCATGGCCA CCGCTGCGAC CGAGGAGCCT 
TTCCCTTTCC ATGGTCTTCT ACCAAAGAAA GAGACTGGGG CCTCCTCCTT CCTGTGCCGC 
TACCCGGAGT ATGACGGACG CGGGGTGCTC ATCGCCGTCC TGGACACAGG GGTTGATCCC 
GGGGCCCCGG GCATGCAGGT CACGACTGAT GGGAAACCAA AAATCATTGA TATCATTGAT 
ACAACAGGAA GTGGTGATGT GAATACTGCT ACAGAAGTAG AACCAAAAGA TGGCGAAATC 
ACTGGTCTTT CTGGAAGAGT GCTTAAGATT CCTGCAAACT GGACAAATCC TTCAGGAAAA 
TATCATATTG GCATTAAAAA TGGTTATGAC TTCTATCCAA AGGCTCTCAA GGAAAGGATA 
CAGAAAGAAC GGAAGGAAAA AATCTGGGAT CCAATTCACA GAGTTGCGCT TGCAGAAGCT 
TGTAGAAAAC AAGAAGAATT TGATATTGCC AATAATGGCT CTTCCCAAGC CAATAAACTA 
ATCAAGGAAG AGTTGCAGAG TCAAGTGGAA TTACTTAATT CTTTTGAGAA AAAGTATAGT 
GATCCAGGCC CTGTGTATGA CTGCTTGGTG TGGCATGATG GTGAGACCTG GAGAGCCTGT 
GTTGATTCGA ATGAAAATGG GGACTTGGGT AAATCTACTG TATTGAGAAA CTACAAAGAG 
GCTCAAGAAT ATGGTTCTTT TGGCACAGCT GAGATGCTGA ATTACTCTGT TAATATTTAT 
GATGATGGAA ACCTGCTCTC CATTGTGACC AGTGGAGGAG CTCATGGAAC CCATGTAGCG 
AGTATAGCTG CTGGGCATTT TCCAGAAGAG CCTGAACGGA ATGGAGTTGC TCCTGGTGCT 
CAAATTCTAT CCATTAAGAT TGGTGATACA AGGCTAAGCA CCATGGAAAC AGGCACAGGC 
CTCATCAGAG CTATGATAGA AGTTATAAAT CATAAGTGTG ATCTTGTCAA CTACAGTTAT 
GGAGAAGCAA CTCATTGGCC AAATTCTGGG AGAATTTGTG AAGTAATTAA TGAAGCAGTA 
TGGAAACATA ATACAATTTA TGTTTCAAGT GCTGGAAATA ATGGTCCATG CCTTTCTACA 
GTGGGTTGTC CAGGAGGAAC TACTTCCAGC GTGATAGGTG TTGGAGCTTA TGTTTCTCCT 
GATATGATGG TTGCAGAGTA TTCACTGAGA GAGAAACTAC CTGCAAATCA ATATACATGG 
TCTTCTAGAG GCCCAAGTGC TGATGGGGCC CTTGGTGTGA GCATCAGTGC ACCAGGAGGT 
GCTATTGCTT CTGTGCCTAA CTGGACATTG AGGGGGACTC AGCTAATGAA TGGGACATCA 
ATGTCTTCCC CCAATGCCTG TGGTGGCATT GCCCTGGTAC TTTCAGGACT GAAAGCAAAT 
AATGTTGACT ATACTGTTCA CTCAGTCAGA AGAGCTCTAG AAAACACTGC AATAAAAGCT 
GACAATATAG AAGTATTTGC CCAAGGACAT GGAATTATTC AGGTTGACAA AGCTTATGAC 
TACCTCATTC AGAATACATC ATTTGCTAAC AGATTAGGTT TTACAGTTAC AGTTGGAAAT 
AACCGTGGCA TCTACCTCCG AGATCCTGTT CAGGTGGCTG CTCCTTCAGA TCATGGTGTT 
GGCATTGAGC CTGTATTTCC AGAGAACACA GAAAACTCTG AAAAAATATC CTTCCAGCTT 
CATTTAGCTT TAACTTCAAA TTCATCTTGG GTTCAATGTC CCAGCCATTT GGAACTCATG 
AATCAATGTC GGCACATAAA CATACGTGTG GACCCCAGGG GCTTAAGAGA AGGGTTACAT 
TATACAGAGG TATGTGGGTA TGATATAGCA TCCCCCAATG CAGGTCCTTT ATTCAGAGTT 
CCAATCACTG CAGTTATAGC AGCAAAAGTA AATGAATCAT CACATTATGA TCTAGCCTTT 
ACAGATGTAC ATTTTAAACC TGGTCAGATT CGAAGACATT TTGTTGAGGT TCCTGAGGGG 
GCAACTTGGG CTGGTAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006244932.1
CDS	1987..4407
Translation	MRIPRLRQTAYSSRDVSWRVTAPNASPGFFAPHRPFKNLPPVRCHWHQRKLHPRSAAWVTNIPVSCLVAAPLAYVEVYDWLDISDRMRLGTGGQAPPLPLFRPAVAGCPRAPRPRAAWQFTSSTSVLQLASMATAATEEPFPFHGLLPKKETGASSFLCRYPEYDGRGVLIAVLDTGVDPGAPGMQVTTDGKPKIIDIIDTTGSGDVNTATEVEPKDGEITGLSGRVLKIPANWTNPSGKYHIGIKNGYDFYPKALKERIQKERKEKIWDPIHRVALAEACRKQEEFDIANNGSSQANKLIKEELQSQVELLNSFEKKYSDPGPVYDCLVWHDGETWRACVDSNENGDLGKSTVLRNYKEAQEYGSFGTAEMLNYSVNIYDDGNLLSIVTSGGAHGTHVASIAAGHFPEEPERNGVAPGAQILSIKIGDTRLSTMETGTGLIRAMIEVINHKCDLVNYSYGEATHWPNSGRICEVINEAVWKHNTIYVSSAGNNGPCLSTVGCPGGTTSSVIGVGAYVSPDMMVAEYSLREKLPANQYTWSSRGPSADGALGVSISAPGGAIASVPNWTLRGTQLMNGTSMSSPNACGGIALVLSGLKANNVDYTVHSVRRALENTAIKADNIEVFAQGHGIIQVDKAYDYLIQNTSFANRLGFTVTVGNNRGIYLRDPVQVAAPSDHGVGIEPVFPENTENSEKISFQLHLALTSNSSWVQCPSHLELMNQCRHINIRVDPRGLREGLHYTEVCGYDIASPNAGPLFRVPITAVIAAKVNESSHYDLAFTDVHFKPGQIRRHFVEVPEGATWAGK

Target ORF information:

RefSeq Version	XM_006244870.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus tripeptidyl peptidase 2 (Tpp2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006244870.3

ORF Insert Sequence:

ATGAGGATCC CACGCCTCAG GCAGACCGCC TATTCCTCTC GCGACGTCTC TTGGAGAGTG 
ACAGCTCCGA ATGCAAGTCC CGGTTTCTTC GCTCCCCACA GACCCTTTAA GAACCTCCCT 
CCAGTGCGTT GTCATTGGCA CCAGCGCAAA CTCCACCCCC GCTCTGCAGC CTGGGTAACA 
AACATTCCTG TGTCTTGCCT CGTTGCGGCG CCGTTAGCTT ACGTTGAAGT ATATGATTGG 
CTAGACATAT CTGACCGCAT GCGATTGGGT ACTGGTGGCC AGGCCCCGCC CCTTCCCCTC 
TTCCGTCCCG CGGTCGCCGG GTGTCCTCGC GCCCCTCGTC CGCGCGCTGC CTGGCAGTTT 
ACCTCTTCCA CGTCCGTCCT CCAGCTTGCG TCCATGGCCA CCGCTGCGAC CGAGGAGCCT 
TTCCCTTTCC ATGGTCTTCT ACCAAAGAAA GAGACTGGGG CCTCCTCCTT CCTGTGCCGC 
TACCCGGAGT ATGACGGACG CGGGGTGCTC ATCGCCGTCC TGGACACAGG GGTTGATCCC 
GGGGCCCCGG GCATGCAGGT CACGACTGAT GGGAAACCAA AAATCATTGA TATCATTGAT 
ACAACAGGAA GTGGTGATGT GAATACTGCT ACAGAAGTAG AACCAAAAGA TGGCGAAATC 
ACTGGTCTTT CTGGAAGAGT GCTTAAGATT CCTGCAAACT GGACAAATCC TTCAGGAAAA 
TATCATATTG GCATTAAAAA TGGTTATGAC TTCTATCCAA AGGCTCTCAA GGAAAGGATA 
CAGAAAGAAC GGAAGGAAAA AATCTGGGAT CCAATTCACA GAGTTGCGCT TGCAGAAGCT 
TGTAGAAAAC AAGAAGAATT TGATATTGCC AATAATGGCT CTTCCCAAGC CAATAAACTA 
ATCAAGGAAG AGTTGCAGAG TCAAGTGGAA TTACTTAATT CTTTTGAGAA AAAGTATAGT 
GATCCAGGCC CTGTGTATGA CTGCTTGGTG TGGCATGATG GTGAGACCTG GAGAGCCTGT 
GTTGATTCGA ATGAAAATGG GGACTTGGGT AAATCTACTG TATTGAGAAA CTACAAAGAG 
GCTCAAGAAT ATGGTTCTTT TGGCACAGCT GAGATGCTGA ATTACTCTGT TAATATTTAT 
GATGATGGAA ACCTGCTCTC CATTGTGACC AGTGGAGGAG CTCATGGAAC CCATGTAGCG 
AGTATAGCTG CTGGGCATTT TCCAGAAGAG CCTGAACGGA ATGGAGTTGC TCCTGGTGCT 
CAAATTCTAT CCATTAAGAT TGGTGATACA AGGCTAAGCA CCATGGAAAC AGGCACAGGC 
CTCATCAGAG CTATGATAGA AGTTATAAAT CATAAGTGTG ATCTTGTCAA CTACAGTTAT 
GGAGAAGCAA CTCATTGGCC AAATTCTGGG AGAATTTGTG AAGTAATTAA TGAAGCAGTA 
TGGAAACATA ATACAATTTA TGTTTCAAGT GCTGGAAATA ATGGTCCATG CCTTTCTACA 
GTGGGTTGTC CAGGAGGAAC TACTTCCAGC GTGATAGGTG TTGGAGCTTA TGTTTCTCCT 
GATATGATGG TTGCAGAGTA TTCACTGAGA GAGAAACTAC CTGCAAATCA ATATACATGG 
TCTTCTAGAG GCCCAAGTGC TGATGGGGCC CTTGGTGTGA GCATCAGTGC ACCAGGAGGT 
GCTATTGCTT CTGTGCCTAA CTGGACATTG AGGGGGACTC AGCTAATGAA TGGGACATCA 
ATGTCTTCCC CCAATGCCTG TGGTGGCATT GCCCTGGTAC TTTCAGGACT GAAAGCAAAT 
AATGTTGACT ATACTGTTCA CTCAGTCAGA AGAGCTCTAG AAAACACTGC AATAAAAGCT 
GACAATATAG AAGTATTTGC CCAAGGACAT GGAATTATTC AGGTTGACAA AGCTTATGAC 
TACCTCATTC AGAATACATC ATTTGCTAAC AGATTAGGTT TTACAGTTAC AGTTGGAAAT 
AACCGTGGCA TCTACCTCCG AGATCCTGTT CAGGTGGCTG CTCCTTCAGA TCATGGTGTT 
GGCATTGAGC CTGTATTTCC AGAGAACACA GAAAACTCTG AAAAAATATC CTTCCAGCTT 
CATTTAGCTT TAACTTCAAA TTCATCTTGG GTTCAATGTC CCAGCCATTT GGAACTCATG 
AATCAATGTC GGCACATAAA CATACGTGTG GACCCCAGGG GCTTAAGAGA AGGGTTACAT 
TATACAGAGG TATGTGGGTA TGATATAGCA TCCCCCAATG CAGGTCCTTT ATTCAGAGTT 
CCAATCACTG CAGTTATAGC AGCAAAAGTA AATGAATCAT CACATTATGA TCTAGCCTTT 
ACAGATGTAC ATTTTAAACC TGGTCAGATT CGAAGACATT TTGTTGAGGT TCCTGAGGGG 
GCAACTTGGG CTGGTAAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tpp2 ( XM_006244852.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006244914.2 Rattus norvegicus tripeptidyl peptidase 2 (Tpp2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa62870
Clone ID Related Accession (Same CDS sequence)	XM_006244852.3
Accession Version	XM_006244852.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3789bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	tripeptidyl-peptidase 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006244852.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACCG CTGCGACCGA GGAGCCTTTC CCTTTCCATG GTCTTCTACC AAAGAAAGAG 
ACTGGGGCCT CCTCCTTCCT GTGCCGCTAC CCGGAGTATG ACGGACGCGG GGTGCTCATC 
GCCGTCCTGG ACACAGGGGT TGATCCCGGG GCCCCGGGCA TGCAGGTCAC GACTGATGGG 
AAACCAAAAA TCATTGATAT CATTGATACA ACAGGAAGTG GTGATGTGAA TACTGCTACA 
GAAGTAGAAC CAAAAGATGG CGAAATCACT GGTCTTTCTG GAAGAGTGCT TAAGATTCCT 
GCAAACTGGA CAAATCCTTC AGGAAAATAT CATATTGGCA TTAAAAATGG TTATGACTTC 
TATCCAAAGG CTCTCAAGGA AAGGATACAG AAAGAACGGA AGGAAAAAAT CTGGGATCCA 
ATTCACAGAG TTGCGCTTGC AGAAGCTTGT AGAAAACAAG AAGAATTTGA TATTGCCAAT 
AATGGCTCTT CCCAAGCCAA TAAACTAATC AAGGAAGAGT TGCAGAGTCA AGTGGAATTA 
CTTAATTCTT TTGAGAAAAA GTATAGTGAT CCAGGCCCTG TGTATGACTG CTTGGTGTGG 
CATGATGGTG AGACCTGGAG AGCCTGTGTT GATTCGAATG AAAATGGGGA CTTGGGTAAA 
TCTACTGTAT TGAGAAACTA CAAAGAGGCT CAAGAATATG GTTCTTTTGG CACAGCTGAG 
ATGCTGAATT ACTCTGTTAA TATTTATGAT GATGGAAACC TGCTCTCCAT TGTGACCAGT 
GGAGGAGCTC ATGGAACCCA TGTAGCGAGT ATAGCTGCTG GGCATTTTCC AGAAGAGCCT 
GAACGGAATG GAGTTGCTCC TGGTGCTCAA ATTCTATCCA TTAAGATTGG TGATACAAGG 
CTAAGCACCA TGGAAACAGG CACAGGCCTC ATCAGAGCTA TGATAGAAGT TATAAATCAT 
AAGTGTGATC TTGTCAACTA CAGTTATGGA GAAGCAACTC ATTGGCCAAA TTCTGGGAGA 
ATTTGTGAAG TAATTAATGA AGCAGTATGG AAACATAATA CAATTTATGT TTCAAGTGCT 
GGAAATAATG GTCCATGCCT TTCTACAGTG GGTTGTCCAG GAGGAACTAC TTCCAGCGTG 
ATAGGTGTTG GAGCTTATGT TTCTCCTGAT ATGATGGTTG CAGAGTATTC ACTGAGAGAG 
AAACTACCTG CAAATCAATA TACATGGTCT TCTAGAGGCC CAAGTGCTGA TGGGGCCCTT 
GGTGTGAGCA TCAGTGCACC AGGAGGTGCT ATTGCTTCTG TGCCTAACTG GACATTGAGG 
GGGACTCAGC TAATGAATGG GACATCAATG TCTTCCCCCA ATGCCTGTGG TGGCATTGCC 
CTGGTACTTT CAGGACTGAA AGCAAATAAT GTTGACTATA CTGTTCACTC AGTCAGAAGA 
GCTCTAGAAA ACACTGCAAT AAAAGCTGAC AATATAGAAG TATTTGCCCA AGGACATGGA 
ATTATTCAGG TTGACAAAGC TTATGACTAC CTCATTCAGA ATACATCATT TGCTAACAGA 
TTAGGTTTTA CAGTTACAGT TGGAAATAAC CGTGGCATCT ACCTCCGAGA TCCTGTTCAG 
GTGGCTGCTC CTTCAGATCA TGGTGTTGGC ATTGAGCCTG TATTTCCAGA GAACACAGAA 
AACTCTGAAA AAATATCCTT CCAGCTTCAT TTAGCTTTAA CTTCAAATTC ATCTTGGGTT 
CAATGTCCCA GCCATTTGGA ACTCATGAAT CAATGTCGGC ACATAAACAT ACGTGTGGAC 
CCCAGGGGCT TAAGAGAAGG GTTACATTAT ACAGAGGTAT GTGGGTATGA TATAGCATCC 
CCCAATGCAG GTCCTTTATT CAGAGTTCCA ATCACTGCAG TTATAGCAGC AAAAGTAAAT 
GAATCATCAC ATTATGATCT AGCCTTTACA GATGTACATT TTAAACCTGG TCAGATTCGA 
AGACATTTTG TTGAGGTTCC TGAGGGGGCA ACTTGGGCTG AAGTTACCGT GTGTTCGTGT 
TCTTCTGAGG TATCAGCAAA ATTTGTTCTT CATGCAGTAC AGCTTGTGAA GCAGAGAGCA 
TATCGAAGTC ATGAATTTTA TAAGTTTTGT TCCCTTCCAG AAAAAGGAAC ACTCATTGAA 
GCCTTTCCTG TTCTGGGTGG GAAAGCAATT GAATTTTGTA TTGCTCGTTG GTGGGCAAGT 
CTCAGTGATG TCAATATTGA TTATACCATA TCATTCCATG GCATAGTGTG TACTGCACCA 
CAGTTAAACA TTCATGCATC TGAAGGAATC AATCGTTTTG ATGTCCAGTC CTCTTTAAAA 
TATGAAGATC TGGCTCCTTG CATAACTTTG AAGAGCTGGG TGCAAACACT ACGCCCAGTA 
AATGCAAAAA CCAGACCTTT AGGATCAAGA GATGTTTTGC CAAATAATCG CCAGCTTTAT 
GAGATGGTCC TGACATACAG CTTTCATCAG CCCAAGAGCG GAGAAGTAAC ACCTAGTTGT 
CCACTCCTTT GTGAATTATT ATATGAGTCA GAATTTGACA GTCAGTTGTG GATTATTTTT 
GACCAGAACA AAAGACAGAT GGGCTCAGGC GATGCCTATC CACATCAGTA TTCTTTGAAA 
TTGGAGAAAG GAGATTATAC GATTCGATTA CAGATTCGTC ATGAGCAAAT CAGTGATTTG 
GATCGTCTCA AAGATCTTCC GTTTATTGTT TCGCATAGGT TGTCTAATAC CTTGAGCTTA 
GATATTCATG AAAATCATAG CCTTGCACTT CTAGGAAAGA AGAAATCAAG CAGTTTGACA 
TTACCACCCA AATATAATCA GCCATTCTTT GTTACTTCCT TACCTGATGA TAAAATACCT 
AAGGGGGCAG GACCCGGATG CTACCTTGCA GGCTCCTTGA CATTGTCAAA GACTGAGCTT 
GGAAAGAAAG CTGGGCAGTC TGCAGCAAAA CGACAAGGAA AATTTAAAAA GGATGTGATC 
CCTGTTCATT ACTATCTAAT ACCTCCACCA ACAAAGACTA AGAATGGCAG CAAAGATAAA 
GAAAAGGATT CAGAAAAAGA GAAAGATTTG AAAGAAGAGT TTACTGAAGC TTTACGAGAT 
CTTAAAATTC AGTGGATGAC CAAGCTGGAT TCTACTGACA TTTACAATGA ATTGAAAGAA 
ACATATCCTG CTTACCTTCC TTTGTATGTT GCACGTCTTC ATCAATTAGA TGCTGAAAAG 
GAGCGGATGA AAAGACTTAA TGAAATTGTT GATGCTGCCA ATGCTGTTAT TTCTCACATC 
GATCAGACCG CTCTCGCTGT TTACATTGCC ATGAAGACTG ACCCCAGGCC TGATGCAGCT 
ACTATAAAAA ATGATATGGA CAAGCAGAAA TCTACCCTGG TAGATGCCCT CTGCAGGAAA 
GGATGTGCTC TGGCAGACCA CCTTCTTCAT GCACAGCCCC ACGATGGGGC AGCAGCTGGA 
GATGCAGAAG CAAAAGAAGA GGAAGGAGAA AGTACCTTGG AATCTCTATC AGAAACCTAT 
TGGGAAACTA CAAAGTGGAC AGATCTTTTT GACACTAAGG TTTTGACATT TGCATACAAG 
CATGCATTAG TAAATAAGAT GTACGGGAGA GGCCTTAAGT TTGCAACCAA ACTCGTAGAA 
GAAAAACCAA CAAAAGAAAA CTGGAAAAAT TGTATTCAAC TAATGAAATT ACTCGGATGG 
ACCCATTGTG CGTCTTTTAC TGAAAACTGG CTCCCCATCA TGTATCCTCC TGATTATTGT 
GTATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006244914.2
CDS	103..3891
Translation	MATAATEEPFPFHGLLPKKETGASSFLCRYPEYDGRGVLIAVLDTGVDPGAPGMQVTTDGKPKIIDIIDTTGSGDVNTATEVEPKDGEITGLSGRVLKIPANWTNPSGKYHIGIKNGYDFYPKALKERIQKERKEKIWDPIHRVALAEACRKQEEFDIANNGSSQANKLIKEELQSQVELLNSFEKKYSDPGPVYDCLVWHDGETWRACVDSNENGDLGKSTVLRNYKEAQEYGSFGTAEMLNYSVNIYDDGNLLSIVTSGGAHGTHVASIAAGHFPEEPERNGVAPGAQILSIKIGDTRLSTMETGTGLIRAMIEVINHKCDLVNYSYGEATHWPNSGRICEVINEAVWKHNTIYVSSAGNNGPCLSTVGCPGGTTSSVIGVGAYVSPDMMVAEYSLREKLPANQYTWSSRGPSADGALGVSISAPGGAIASVPNWTLRGTQLMNGTSMSSPNACGGIALVLSGLKANNVDYTVHSVRRALENTAIKADNIEVFAQGHGIIQVDKAYDYLIQNTSFANRLGFTVTVGNNRGIYLRDPVQVAAPSDHGVGIEPVFPENTENSEKISFQLHLALTSNSSWVQCPSHLELMNQCRHINIRVDPRGLREGLHYTEVCGYDIASPNAGPLFRVPITAVIAAKVNESSHYDLAFTDVHFKPGQIRRHFVEVPEGATWAEVTVCSCSSEVSAKFVLHAVQLVKQRAYRSHEFYKFCSLPEKGTLIEAFPVLGGKAIEFCIARWWASLSDVNIDYTISFHGIVCTAPQLNIHASEGINRFDVQSSLKYEDLAPCITLKSWVQTLRPVNAKTRPLGSRDVLPNNRQLYEMVLTYSFHQPKSGEVTPSCPLLCELLYESEFDSQLWIIFDQNKRQMGSGDAYPHQYSLKLEKGDYTIRLQIRHEQISDLDRLKDLPFIVSHRLSNTLSLDIHENHSLALLGKKKSSSLTLPPKYNQPFFVTSLPDDKIPKGAGPGCYLAGSLTLSKTELGKKAGQSAAKRQGKFKKDVIPVHYYLIPPPTKTKNGSKDKEKDSEKEKDLKEEFTEALRDLKIQWMTKLDSTDIYNELKETYPAYLPLYVARLHQLDAEKERMKRLNEIVDAANAVISHIDQTALAVYIAMKTDPRPDAATIKNDMDKQKSTLVDALCRKGCALADHLLHAQPHDGAAAGDAEAKEEEGESTLESLSETYWETTKWTDLFDTKVLTFAYKHALVNKMYGRGLKFATKLVEEKPTKENWKNCIQLMKLLGWTHCASFTENWLPIMYPPDYCVF

Target ORF information:

RefSeq Version	XM_006244852.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus tripeptidyl peptidase 2 (Tpp2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006244852.3

ORF Insert Sequence:

ATGGCCACCG CTGCGACCGA GGAGCCTTTC CCTTTCCATG GTCTTCTACC AAAGAAAGAG 
ACTGGGGCCT CCTCCTTCCT GTGCCGCTAC CCGGAGTATG ACGGACGCGG GGTGCTCATC 
GCCGTCCTGG ACACAGGGGT TGATCCCGGG GCCCCGGGCA TGCAGGTCAC GACTGATGGG 
AAACCAAAAA TCATTGATAT CATTGATACA ACAGGAAGTG GTGATGTGAA TACTGCTACA 
GAAGTAGAAC CAAAAGATGG CGAAATCACT GGTCTTTCTG GAAGAGTGCT TAAGATTCCT 
GCAAACTGGA CAAATCCTTC AGGAAAATAT CATATTGGCA TTAAAAATGG TTATGACTTC 
TATCCAAAGG CTCTCAAGGA AAGGATACAG AAAGAACGGA AGGAAAAAAT CTGGGATCCA 
ATTCACAGAG TTGCGCTTGC AGAAGCTTGT AGAAAACAAG AAGAATTTGA TATTGCCAAT 
AATGGCTCTT CCCAAGCCAA TAAACTAATC AAGGAAGAGT TGCAGAGTCA AGTGGAATTA 
CTTAATTCTT TTGAGAAAAA GTATAGTGAT CCAGGCCCTG TGTATGACTG CTTGGTGTGG 
CATGATGGTG AGACCTGGAG AGCCTGTGTT GATTCGAATG AAAATGGGGA CTTGGGTAAA 
TCTACTGTAT TGAGAAACTA CAAAGAGGCT CAAGAATATG GTTCTTTTGG CACAGCTGAG 
ATGCTGAATT ACTCTGTTAA TATTTATGAT GATGGAAACC TGCTCTCCAT TGTGACCAGT 
GGAGGAGCTC ATGGAACCCA TGTAGCGAGT ATAGCTGCTG GGCATTTTCC AGAAGAGCCT 
GAACGGAATG GAGTTGCTCC TGGTGCTCAA ATTCTATCCA TTAAGATTGG TGATACAAGG 
CTAAGCACCA TGGAAACAGG CACAGGCCTC ATCAGAGCTA TGATAGAAGT TATAAATCAT 
AAGTGTGATC TTGTCAACTA CAGTTATGGA GAAGCAACTC ATTGGCCAAA TTCTGGGAGA 
ATTTGTGAAG TAATTAATGA AGCAGTATGG AAACATAATA CAATTTATGT TTCAAGTGCT 
GGAAATAATG GTCCATGCCT TTCTACAGTG GGTTGTCCAG GAGGAACTAC TTCCAGCGTG 
ATAGGTGTTG GAGCTTATGT TTCTCCTGAT ATGATGGTTG CAGAGTATTC ACTGAGAGAG 
AAACTACCTG CAAATCAATA TACATGGTCT TCTAGAGGCC CAAGTGCTGA TGGGGCCCTT 
GGTGTGAGCA TCAGTGCACC AGGAGGTGCT ATTGCTTCTG TGCCTAACTG GACATTGAGG 
GGGACTCAGC TAATGAATGG GACATCAATG TCTTCCCCCA ATGCCTGTGG TGGCATTGCC 
CTGGTACTTT CAGGACTGAA AGCAAATAAT GTTGACTATA CTGTTCACTC AGTCAGAAGA 
GCTCTAGAAA ACACTGCAAT AAAAGCTGAC AATATAGAAG TATTTGCCCA AGGACATGGA 
ATTATTCAGG TTGACAAAGC TTATGACTAC CTCATTCAGA ATACATCATT TGCTAACAGA 
TTAGGTTTTA CAGTTACAGT TGGAAATAAC CGTGGCATCT ACCTCCGAGA TCCTGTTCAG 
GTGGCTGCTC CTTCAGATCA TGGTGTTGGC ATTGAGCCTG TATTTCCAGA GAACACAGAA 
AACTCTGAAA AAATATCCTT CCAGCTTCAT TTAGCTTTAA CTTCAAATTC ATCTTGGGTT 
CAATGTCCCA GCCATTTGGA ACTCATGAAT CAATGTCGGC ACATAAACAT ACGTGTGGAC 
CCCAGGGGCT TAAGAGAAGG GTTACATTAT ACAGAGGTAT GTGGGTATGA TATAGCATCC 
CCCAATGCAG GTCCTTTATT CAGAGTTCCA ATCACTGCAG TTATAGCAGC AAAAGTAAAT 
GAATCATCAC ATTATGATCT AGCCTTTACA GATGTACATT TTAAACCTGG TCAGATTCGA 
AGACATTTTG TTGAGGTTCC TGAGGGGGCA ACTTGGGCTG AAGTTACCGT GTGTTCGTGT 
TCTTCTGAGG TATCAGCAAA ATTTGTTCTT CATGCAGTAC AGCTTGTGAA GCAGAGAGCA 
TATCGAAGTC ATGAATTTTA TAAGTTTTGT TCCCTTCCAG AAAAAGGAAC ACTCATTGAA 
GCCTTTCCTG TTCTGGGTGG GAAAGCAATT GAATTTTGTA TTGCTCGTTG GTGGGCAAGT 
CTCAGTGATG TCAATATTGA TTATACCATA TCATTCCATG GCATAGTGTG TACTGCACCA 
CAGTTAAACA TTCATGCATC TGAAGGAATC AATCGTTTTG ATGTCCAGTC CTCTTTAAAA 
TATGAAGATC TGGCTCCTTG CATAACTTTG AAGAGCTGGG TGCAAACACT ACGCCCAGTA 
AATGCAAAAA CCAGACCTTT AGGATCAAGA GATGTTTTGC CAAATAATCG CCAGCTTTAT 
GAGATGGTCC TGACATACAG CTTTCATCAG CCCAAGAGCG GAGAAGTAAC ACCTAGTTGT 
CCACTCCTTT GTGAATTATT ATATGAGTCA GAATTTGACA GTCAGTTGTG GATTATTTTT 
GACCAGAACA AAAGACAGAT GGGCTCAGGC GATGCCTATC CACATCAGTA TTCTTTGAAA 
TTGGAGAAAG GAGATTATAC GATTCGATTA CAGATTCGTC ATGAGCAAAT CAGTGATTTG 
GATCGTCTCA AAGATCTTCC GTTTATTGTT TCGCATAGGT TGTCTAATAC CTTGAGCTTA 
GATATTCATG AAAATCATAG CCTTGCACTT CTAGGAAAGA AGAAATCAAG CAGTTTGACA 
TTACCACCCA AATATAATCA GCCATTCTTT GTTACTTCCT TACCTGATGA TAAAATACCT 
AAGGGGGCAG GACCCGGATG CTACCTTGCA GGCTCCTTGA CATTGTCAAA GACTGAGCTT 
GGAAAGAAAG CTGGGCAGTC TGCAGCAAAA CGACAAGGAA AATTTAAAAA GGATGTGATC 
CCTGTTCATT ACTATCTAAT ACCTCCACCA ACAAAGACTA AGAATGGCAG CAAAGATAAA 
GAAAAGGATT CAGAAAAAGA GAAAGATTTG AAAGAAGAGT TTACTGAAGC TTTACGAGAT 
CTTAAAATTC AGTGGATGAC CAAGCTGGAT TCTACTGACA TTTACAATGA ATTGAAAGAA 
ACATATCCTG CTTACCTTCC TTTGTATGTT GCACGTCTTC ATCAATTAGA TGCTGAAAAG 
GAGCGGATGA AAAGACTTAA TGAAATTGTT GATGCTGCCA ATGCTGTTAT TTCTCACATC 
GATCAGACCG CTCTCGCTGT TTACATTGCC ATGAAGACTG ACCCCAGGCC TGATGCAGCT 
ACTATAAAAA ATGATATGGA CAAGCAGAAA TCTACCCTGG TAGATGCCCT CTGCAGGAAA 
GGATGTGCTC TGGCAGACCA CCTTCTTCAT GCACAGCCCC ACGATGGGGC AGCAGCTGGA 
GATGCAGAAG CAAAAGAAGA GGAAGGAGAA AGTACCTTGG AATCTCTATC AGAAACCTAT 
TGGGAAACTA CAAAGTGGAC AGATCTTTTT GACACTAAGG TTTTGACATT TGCATACAAG 
CATGCATTAG TAAATAAGAT GTACGGGAGA GGCCTTAAGT TTGCAACCAA ACTCGTAGAA 
GAAAAACCAA CAAAAGAAAA CTGGAAAAAT TGTATTCAAC TAATGAAATT ACTCGGATGG 
ACCCATTGTG CGTCTTTTAC TGAAAACTGG CTCCCCATCA TGTATCCTCC TGATTATTGT 
GTATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tpp2 ( XM_006244853.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006244915.2 Rattus norvegicus tripeptidyl peptidase 2 (Tpp2), transcript variant X3, mRNA.

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CloneID	ORa62871
Clone ID Related Accession (Same CDS sequence)	XM_006244853.3
Accession Version	XM_006244853.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3414bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	tripeptidyl-peptidase 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006244853.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCACCG CTGCGACCGA GGAGCCTTTC CCTTTCCATG GTCTTCTACC AAAGAAAGAG 
ACTGGGGCCT CCTCCTTCCT GTGCCGCTAC CCGGAGTATG ACGGACGCGG GGTGCTCATC 
GCCGTCCTGG ACACAGGGGT TGATCCCGGG GCCCCGGGCA TGCAGGTCAC GACTGATGGG 
AAACCAAAAA TCATTGATAT CATTGATACA ACAGGAAGTG GTGATGTGAA TACTGCTACA 
GAAGTAGAAC CAAAAGATGG CGAAATCACT GGTCTTTCTG GAAGAGTGCT TAAGATTCCT 
GCAAACTGGA CAAATCCTTC AGGAAAATAT CATATTGGCA TTAAAAATGG TTATGACTTC 
TATCCAAAGG CTCTCAAGGA AAGGATACAG AAAGAACGGA AGGAAAAAAT CTGGGATCCA 
ATTCACAGAG TTGCGCTTGC AGAAGCTTGT AGAAAACAAG AAGAATTTGA TATTGCCAAT 
AATGGCTCTT CCCAAGCCAA TAAACTAATC AAGGAAGAGT TGCAGAGTCA AGTGGAATTA 
CTTAATTCTT TTGAGAAAAA GTATAGTGAT CCAGGCCCTG TGTATGACTG CTTGGTGTGG 
CATGATGGTG AGACCTGGAG AGCCTGTGTT GATTCGAATG AAAATGGGGA CTTGGGTAAA 
TCTACTGTAT TGAGAAACTA CAAAGAGGCT CAAGAATATG GTTCTTTTGG CACAGCTGAG 
ATGCTGAATT ACTCTGTTAA TATTTATGAT GATGGAAACC TGCTCTCCAT TGTGACCAGT 
GGAGGAGCTC ATGGAACCCA TGTAGCGAGT ATAGCTGCTG GGCATTTTCC AGAAGAGCCT 
GAACGGAATG GAGTTGCTCC TGGTGCTCAA ATTCTATCCA TTAAGATTGG TGATACAAGG 
CTAAGCACCA TGGAAACAGG CACAGGCCTC ATCAGAGCTA TGATAGAAGT TATAAATCAT 
AAGTGTGATC TTGTCAACTA CAGTTATGGA GAAGCAACTC ATTGGCCAAA TTCTGGGAGA 
ATTTGTGAAG TAATTAATGA AGCAGTATGG AAACATAATA CAATTTATGT TTCAAGTGCT 
GGAAATAATG GTCCATGCCT TTCTACAGTG GGTTGTCCAG GAGGAACTAC TTCCAGCGTG 
ATAGGTGTTG GAGCTTATGT TTCTCCTGAT ATGATGGTTG CAGAGTATTC ACTGAGAGAG 
AAACTACCTG CAAATCAATA TACATGGTCT TCTAGAGGCC CAAGTGCTGA TGGGGCCCTT 
GGTGTGAGCA TCAGTGCACC AGGAGGTGCT ATTGCTTCTG TGCCTAACTG GACATTGAGG 
GGGACTCAGC TAATGAATGG GACATCAATG TCTTCCCCCA ATGCCTGTGG TGGCATTGCC 
CTGGTACTTT CAGGACTGAA AGCAAATAAT GTTGACTATA CTGTTCACTC AGTCAGAAGA 
GCTCTAGAAA ACACTGCAAT AAAAGCTGAC AATATAGAAG TATTTGCCCA AGGACATGGA 
ATTATTCAGG TTGACAAAGC TTATGACTAC CTCATTCAGA ATACATCATT TGCTAACAGA 
TTAGGTTTTA CAGTTACAGT TGGAAATAAC CGTGGCATCT ACCTCCGAGA TCCTGTTCAG 
GTGGCTGCTC CTTCAGATCA TGGTGTTGGC ATTGAGCCTG TATTTCCAGA GAACACAGAA 
AACTCTGAAA AAATATCCTT CCAGCTTCAT TTAGCTTTAA CTTCAAATTC ATCTTGGGTT 
CAATGTCCCA GCCATTTGGA ACTCATGAAT CAATGTCGGC ACATAAACAT ACGTGTGGAC 
CCCAGGGGCT TAAGAGAAGG GTTACATTAT ACAGAGGTAT GTGGGTATGA TATAGCATCC 
CCCAATGCAG GTCCTTTATT CAGAGTTCCA ATCACTGCAG TTATAGCAGC AAAAGTAAAT 
GAATCATCAC ATTATGATCT AGCCTTTACA GATGTACATT TTAAACCTGG TCAGATTCGA 
AGACATTTTG TTGAGGTTCC TGAGGGGGCA ACTTGGGCTG AAGTTACCGT GTGTTCGTGT 
TCTTCTGAGG TATCAGCAAA ATTTGTTCTT CATGCAGTAC AGCTTGTGAA GCAGAGAGCA 
TATCGAAGTC ATGAATTTTA TAAGTTTTGT TCCCTTCCAG AAAAAGGAAC ACTCATTGAA 
GCCTTTCCTG TTCTGGGTGG GAAAGCAATT GAATTTTGTA TTGCTCGTTG GTGGGCAAGT 
CTCAGTGATG TCAATATTGA TTATACCATA TCATTCCATG GCATAGTGTG TACTGCACCA 
CAGTTAAACA TTCATGCATC TGAAGGAATC AATCGTTTTG ATGTCCAGTC CTCTTTAAAA 
TATGAAGATC TGGCTCCTTG CATAACTTTG AAGAGCTGGG TGCAAACACT ACGCCCAGTA 
AATGCAAAAA CCAGACCTTT AGGATCAAGA GATGTTTTGC CAAATAATCG CCAGCTTTAT 
GAGATGGTCC TGACATACAG CTTTCATCAG CCCAAGAGCG GAGAAGTAAC ACCTAGTTGT 
CCACTCCTTT GTGAATTATT ATATGAGTCA GAATTTGACA GTCAGTTGTG GATTATTTTT 
GACCAGAACA AAAGACAGAT GGGCTCAGGC GATGCCTATC CACATCAGTA TTCTTTGAAA 
TTGGAGAAAG GAGATTATAC GATTCGATTA CAGATTCGTC ATGAGCAAAT CAGTGATTTG 
GATCGTCTCA AAGATCTTCC GTTTATTGTT TCGCATAGGT TGTCTAATAC CTTGAGCTTA 
GATATTCATG AAAATCATAG CCTTGCACTT CTAGGAAAGA AGAAATCAAG CAGTTTGACA 
TTACCACCCA AATATAATCA GCCATTCTTT GTTACTTCCT TACCTGATGA TAAAATACCT 
AAGGGGGCAG GACCCGGATG CTACCTTGCA GGCTCCTTGA CATTGTCAAA GACTGAGCTT 
GGAAAGAAAG CTGGGCAGTC TGCAGCAAAA CGACAAGGAA AATTTAAAAA GGATGTGATC 
CCTGTTCATT ACTATCTAAT ACCTCCACCA ACAAAGACTA AGAATGGCAG CAAAGATAAA 
GAAAAGGATT CAGAAAAAGA GAAAGATTTG AAAGAAGAGT TTACTGAAGC TTTACGAGAT 
CTTAAAATTC AGTGGATGAC CAAGCTGGAT TCTACTGACA TTTACAATGA ATTGAAAGAA 
ACATATCCTG CTTACCTTCC TTTGTATGTT GCACGTCTTC ATCAATTAGA TGCTGAAAAG 
GAGCGGATGA AAAGACTTAA TGAAATTGTT GATGCTGCCA ATGCTGTTAT TTCTCACATC 
GATCAGACCG CTCTCGCTGT TTACATTGCC ATGAAGACTG ACCCCAGGCC TGATGCAGCT 
ACTATAAAAA AGTACCTGAC CATGATATGG ACAAGCAGAA ATCTACCCTG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006244915.2
CDS	103..3516
Translation	MATAATEEPFPFHGLLPKKETGASSFLCRYPEYDGRGVLIAVLDTGVDPGAPGMQVTTDGKPKIIDIIDTTGSGDVNTATEVEPKDGEITGLSGRVLKIPANWTNPSGKYHIGIKNGYDFYPKALKERIQKERKEKIWDPIHRVALAEACRKQEEFDIANNGSSQANKLIKEELQSQVELLNSFEKKYSDPGPVYDCLVWHDGETWRACVDSNENGDLGKSTVLRNYKEAQEYGSFGTAEMLNYSVNIYDDGNLLSIVTSGGAHGTHVASIAAGHFPEEPERNGVAPGAQILSIKIGDTRLSTMETGTGLIRAMIEVINHKCDLVNYSYGEATHWPNSGRICEVINEAVWKHNTIYVSSAGNNGPCLSTVGCPGGTTSSVIGVGAYVSPDMMVAEYSLREKLPANQYTWSSRGPSADGALGVSISAPGGAIASVPNWTLRGTQLMNGTSMSSPNACGGIALVLSGLKANNVDYTVHSVRRALENTAIKADNIEVFAQGHGIIQVDKAYDYLIQNTSFANRLGFTVTVGNNRGIYLRDPVQVAAPSDHGVGIEPVFPENTENSEKISFQLHLALTSNSSWVQCPSHLELMNQCRHINIRVDPRGLREGLHYTEVCGYDIASPNAGPLFRVPITAVIAAKVNESSHYDLAFTDVHFKPGQIRRHFVEVPEGATWAEVTVCSCSSEVSAKFVLHAVQLVKQRAYRSHEFYKFCSLPEKGTLIEAFPVLGGKAIEFCIARWWASLSDVNIDYTISFHGIVCTAPQLNIHASEGINRFDVQSSLKYEDLAPCITLKSWVQTLRPVNAKTRPLGSRDVLPNNRQLYEMVLTYSFHQPKSGEVTPSCPLLCELLYESEFDSQLWIIFDQNKRQMGSGDAYPHQYSLKLEKGDYTIRLQIRHEQISDLDRLKDLPFIVSHRLSNTLSLDIHENHSLALLGKKKSSSLTLPPKYNQPFFVTSLPDDKIPKGAGPGCYLAGSLTLSKTELGKKAGQSAAKRQGKFKKDVIPVHYYLIPPPTKTKNGSKDKEKDSEKEKDLKEEFTEALRDLKIQWMTKLDSTDIYNELKETYPAYLPLYVARLHQLDAEKERMKRLNEIVDAANAVISHIDQTALAVYIAMKTDPRPDAATIKKYLTMIWTSRNLPW

Target ORF information:

RefSeq Version	XM_006244853.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus tripeptidyl peptidase 2 (Tpp2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006244853.3

ORF Insert Sequence:

ATGGCCACCG CTGCGACCGA GGAGCCTTTC CCTTTCCATG GTCTTCTACC AAAGAAAGAG 
ACTGGGGCCT CCTCCTTCCT GTGCCGCTAC CCGGAGTATG ACGGACGCGG GGTGCTCATC 
GCCGTCCTGG ACACAGGGGT TGATCCCGGG GCCCCGGGCA TGCAGGTCAC GACTGATGGG 
AAACCAAAAA TCATTGATAT CATTGATACA ACAGGAAGTG GTGATGTGAA TACTGCTACA 
GAAGTAGAAC CAAAAGATGG CGAAATCACT GGTCTTTCTG GAAGAGTGCT TAAGATTCCT 
GCAAACTGGA CAAATCCTTC AGGAAAATAT CATATTGGCA TTAAAAATGG TTATGACTTC 
TATCCAAAGG CTCTCAAGGA AAGGATACAG AAAGAACGGA AGGAAAAAAT CTGGGATCCA 
ATTCACAGAG TTGCGCTTGC AGAAGCTTGT AGAAAACAAG AAGAATTTGA TATTGCCAAT 
AATGGCTCTT CCCAAGCCAA TAAACTAATC AAGGAAGAGT TGCAGAGTCA AGTGGAATTA 
CTTAATTCTT TTGAGAAAAA GTATAGTGAT CCAGGCCCTG TGTATGACTG CTTGGTGTGG 
CATGATGGTG AGACCTGGAG AGCCTGTGTT GATTCGAATG AAAATGGGGA CTTGGGTAAA 
TCTACTGTAT TGAGAAACTA CAAAGAGGCT CAAGAATATG GTTCTTTTGG CACAGCTGAG 
ATGCTGAATT ACTCTGTTAA TATTTATGAT GATGGAAACC TGCTCTCCAT TGTGACCAGT 
GGAGGAGCTC ATGGAACCCA TGTAGCGAGT ATAGCTGCTG GGCATTTTCC AGAAGAGCCT 
GAACGGAATG GAGTTGCTCC TGGTGCTCAA ATTCTATCCA TTAAGATTGG TGATACAAGG 
CTAAGCACCA TGGAAACAGG CACAGGCCTC ATCAGAGCTA TGATAGAAGT TATAAATCAT 
AAGTGTGATC TTGTCAACTA CAGTTATGGA GAAGCAACTC ATTGGCCAAA TTCTGGGAGA 
ATTTGTGAAG TAATTAATGA AGCAGTATGG AAACATAATA CAATTTATGT TTCAAGTGCT 
GGAAATAATG GTCCATGCCT TTCTACAGTG GGTTGTCCAG GAGGAACTAC TTCCAGCGTG 
ATAGGTGTTG GAGCTTATGT TTCTCCTGAT ATGATGGTTG CAGAGTATTC ACTGAGAGAG 
AAACTACCTG CAAATCAATA TACATGGTCT TCTAGAGGCC CAAGTGCTGA TGGGGCCCTT 
GGTGTGAGCA TCAGTGCACC AGGAGGTGCT ATTGCTTCTG TGCCTAACTG GACATTGAGG 
GGGACTCAGC TAATGAATGG GACATCAATG TCTTCCCCCA ATGCCTGTGG TGGCATTGCC 
CTGGTACTTT CAGGACTGAA AGCAAATAAT GTTGACTATA CTGTTCACTC AGTCAGAAGA 
GCTCTAGAAA ACACTGCAAT AAAAGCTGAC AATATAGAAG TATTTGCCCA AGGACATGGA 
ATTATTCAGG TTGACAAAGC TTATGACTAC CTCATTCAGA ATACATCATT TGCTAACAGA 
TTAGGTTTTA CAGTTACAGT TGGAAATAAC CGTGGCATCT ACCTCCGAGA TCCTGTTCAG 
GTGGCTGCTC CTTCAGATCA TGGTGTTGGC ATTGAGCCTG TATTTCCAGA GAACACAGAA 
AACTCTGAAA AAATATCCTT CCAGCTTCAT TTAGCTTTAA CTTCAAATTC ATCTTGGGTT 
CAATGTCCCA GCCATTTGGA ACTCATGAAT CAATGTCGGC ACATAAACAT ACGTGTGGAC 
CCCAGGGGCT TAAGAGAAGG GTTACATTAT ACAGAGGTAT GTGGGTATGA TATAGCATCC 
CCCAATGCAG GTCCTTTATT CAGAGTTCCA ATCACTGCAG TTATAGCAGC AAAAGTAAAT 
GAATCATCAC ATTATGATCT AGCCTTTACA GATGTACATT TTAAACCTGG TCAGATTCGA 
AGACATTTTG TTGAGGTTCC TGAGGGGGCA ACTTGGGCTG AAGTTACCGT GTGTTCGTGT 
TCTTCTGAGG TATCAGCAAA ATTTGTTCTT CATGCAGTAC AGCTTGTGAA GCAGAGAGCA 
TATCGAAGTC ATGAATTTTA TAAGTTTTGT TCCCTTCCAG AAAAAGGAAC ACTCATTGAA 
GCCTTTCCTG TTCTGGGTGG GAAAGCAATT GAATTTTGTA TTGCTCGTTG GTGGGCAAGT 
CTCAGTGATG TCAATATTGA TTATACCATA TCATTCCATG GCATAGTGTG TACTGCACCA 
CAGTTAAACA TTCATGCATC TGAAGGAATC AATCGTTTTG ATGTCCAGTC CTCTTTAAAA 
TATGAAGATC TGGCTCCTTG CATAACTTTG AAGAGCTGGG TGCAAACACT ACGCCCAGTA 
AATGCAAAAA CCAGACCTTT AGGATCAAGA GATGTTTTGC CAAATAATCG CCAGCTTTAT 
GAGATGGTCC TGACATACAG CTTTCATCAG CCCAAGAGCG GAGAAGTAAC ACCTAGTTGT 
CCACTCCTTT GTGAATTATT ATATGAGTCA GAATTTGACA GTCAGTTGTG GATTATTTTT 
GACCAGAACA AAAGACAGAT GGGCTCAGGC GATGCCTATC CACATCAGTA TTCTTTGAAA 
TTGGAGAAAG GAGATTATAC GATTCGATTA CAGATTCGTC ATGAGCAAAT CAGTGATTTG 
GATCGTCTCA AAGATCTTCC GTTTATTGTT TCGCATAGGT TGTCTAATAC CTTGAGCTTA 
GATATTCATG AAAATCATAG CCTTGCACTT CTAGGAAAGA AGAAATCAAG CAGTTTGACA 
TTACCACCCA AATATAATCA GCCATTCTTT GTTACTTCCT TACCTGATGA TAAAATACCT 
AAGGGGGCAG GACCCGGATG CTACCTTGCA GGCTCCTTGA CATTGTCAAA GACTGAGCTT 
GGAAAGAAAG CTGGGCAGTC TGCAGCAAAA CGACAAGGAA AATTTAAAAA GGATGTGATC 
CCTGTTCATT ACTATCTAAT ACCTCCACCA ACAAAGACTA AGAATGGCAG CAAAGATAAA 
GAAAAGGATT CAGAAAAAGA GAAAGATTTG AAAGAAGAGT TTACTGAAGC TTTACGAGAT 
CTTAAAATTC AGTGGATGAC CAAGCTGGAT TCTACTGACA TTTACAATGA ATTGAAAGAA 
ACATATCCTG CTTACCTTCC TTTGTATGTT GCACGTCTTC ATCAATTAGA TGCTGAAAAG 
GAGCGGATGA AAAGACTTAA TGAAATTGTT GATGCTGCCA ATGCTGTTAT TTCTCACATC 
GATCAGACCG CTCTCGCTGT TTACATTGCC ATGAAGACTG ACCCCAGGCC TGATGCAGCT 
ACTATAAAAA AGTACCTGAC CATGATATGG ACAAGCAGAA ATCTACCCTG GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tpp2 ( NM_031137.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_112399.1 Rattus norvegicus tripeptidyl peptidase 2 (Tpp2), mRNA.

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CloneID	ORa07716
Clone ID Related Accession (Same CDS sequence)	NM_031137.1
Accession Version	NM_031137.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3750bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-02
Organism	Rattus norvegicus(Norway rat)
Product	tripeptidyl-peptidase 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U50194.1. On Jun 26, 2012 this sequence version replaced XM_003750693.1. ##Evidence-Data-START## Transcript exon combination :: U50194.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCCACCG CTGCGACCGA GGAGCCTTTC CCTTTCCATG GTCTTCTACC AAAGAAAGAG 
ACTGGGGCCT CCTCCTTCCT GTGCCGCTAC CCGGAGTATG ACGGACGCGG GGTGCTCATC 
GCCGTCCTGG ACACAGGGGT TGATCCCGGG GCCCCGGGCA TGCAGGTCAC GACTGATGGG 
AAACCAAAAA TCATTGATAT CATTGATACA ACAGGAAGTG GTGATGTGAA TACTGCTACA 
GAAGTAGAAC CAAAAGATGG CGAAATCACT GGTCTTTCTG GAAGAGTGCT TAAGATTCCT 
GCAAACTGGA CAAATCCTTC AGGAAAATAT CATATTGGCA TTAAAAATGG TTATGACTTC 
TATCCAAAGG CTCTCAAGGA AAGGATACAG AAAGAACGGA AGGAAAAAAT CTGGGATCCA 
ATTCACAGAG TTGCGCTTGC AGAAGCTTGT AGAAAACAAG AAGAATTTGA TATTGCCAAT 
AATGGCTCTT CCCAAGCCAA TAAACTAATC AAGGAAGAGT TGCAGAGTCA AGTGGAATTA 
CTTAATTCTT TTGAGAAAAA GTATAGTGAT CCAGGCCCTG TGTATGACTG CTTGGTGTGG 
CATGATGGTG AGACCTGGAG AGCCTGTGTT GATTCGAATG AAAATGGGGA CTTGGGTAAA 
TCTACTGTAT TGAGAAACTA CAAAGAGGCT CAAGAATATG GTTCTTTTGG CACAGCTGAG 
ATGCTGAATT ACTCTGTTAA TATTTATGAT GATGGAAACC TGCTCTCCAT TGTGACCAGT 
GGAGGAGCTC ATGGAACCCA TGTAGCGAGT ATAGCTGCTG GGCATTTTCC AGAAGAGCCT 
GAACGGAATG GAGTTGCTCC TGGTGCTCAA ATTCTATCCA TTAAGATTGG TGATACAAGG 
CTAAGCACCA TGGAAACAGG CACAGGCCTC ATCAGAGCTA TGATAGAAGT TATAAATCAT 
AAGTGTGATC TTGTCAACTA CAGTTATGGA GAAGCAACTC ATTGGCCAAA TTCTGGGAGA 
ATTTGTGAAG TAATTAATGA AGCAGTATGG AAACATAATA CAATTTATGT TTCAAGTGCT 
GGAAATAATG GTCCATGCCT TTCTACAGTG GGTTGTCCAG GAGGAACTAC TTCCAGCGTG 
ATAGGTGTTG GAGCTTATGT TTCTCCTGAT ATGATGGTTG CAGAGTATTC ACTGAGAGAG 
AAACTACCTG CAAATCAATA TACATGGTCT TCTAGAGGCC CAAGTGCTGA TGGGGCCCTT 
GGTGTGAGCA TCAGTGCACC AGGAGGTGCT ATTGCTTCTG TGCCTAACTG GACATTGAGG 
GGGACTCAGC TAATGAATGG GACATCAATG TCTTCCCCCA ATGCCTGTGG TGGCATTGCC 
CTGGTACTTT CAGGGCTGAA AGCAAATAAT GTTGACTATA CTGTTCACTC AGTCAGAAGA 
GCTCTAGAAA ACACTGCAAT AAAAGCTGAC AATATAGAAG TATTTGCCCA AGGACATGGA 
ATTATTCAGG TTGACAAAGC TTATGACTAC CTCATTCAGA ATACATCATT TGCTAACAGA 
TTAGGTTTTA CAGTTACAGT TGGAAATAAC CGTGGCATCT ACCTCCGAGA TCCTGTTCAG 
GTGGCTGCTC CTTCAGATCA TGGTGTTGGC ATTGAGCCTG TATTTCCAGA GAACACAGAA 
AACTCTGAAA AAATATCCTT CCAGCTTCAT TTAGCTTTAA CTTCAAATTC ATCTTGGGTT 
CAATGTCCCA GCCATTTGGA ACTCATGAAT CAATGTCGGC ACATAAACAT ACGTGTGGAC 
CCCAGGGGCT TAAGAGAAGG GTTACATTAT ACAGAGGTAT GTGGGTATGA TATAGCATCC 
CCCAATGCAG GTCCTTTATT CAGAGTTCCA ATCACTGCAG TTATAGCAGC AAAAGTAAAT 
GAATCATCAC ATTATGATCT AGCCTTTACA GATGTACATT TTAAACCTGG TCAGATTCGA 
AGACATTTTG TTGAGGTTCC TGAGGGGGCA ACTTGGGCTG AAGTTACCGT GTGTTCGTGT 
TCTTCTGAGG TATCAGCAAA ATTTGTTCTT CATGCAGTAC AGCTTGTGAA GCAGAGAGCA 
TATCGAAGTC ATGAATTTTA TAAGTTTTGT TCCCTTCCAG AAAAAGGAAC ACTCATTGAA 
GCCTTTCCTG TTCTGGGTGG GAAAGCAATT GAATTTTGTA TTGCTCGTTG GTGGGCAAGT 
CTCAGTGATG TCAATATTGA TTATACCATA TCATTCCATG GCATAGTGTG TACTGCACCA 
CAGTTAAACA TTCATGCATC TGAAGGAATC AATCGTTTTG ATGTCCAGTC CTCTTTAAAA 
TATGAAGATC TGGCTCCTTG CATAACTTTG AAGAGCTGGG TGCAAACACT ACGCCCAGTA 
AATGCAAAAA CCAGACCTTT AGGATCAAGA GATGTTTTGC CAAATAATCG CCAGCTTTAT 
GAGATGGTCC TGACATACAG CTTTCATCAG CCCAAGAGCG GAGAAGTAAC ACCTAGTTGT 
CCACTCCTTT GTGAATTATT ATATGAGTCA GAATTTGACA GTCAGTTGTG GATTATTTTT 
GACCAGAACA AAAGACAGAT GGGCTCAGGC GATGCCTATC CACATCAGTA TTCTTTGAAA 
TTGGAGAAAG GAGATTATAC GATTCGATTA CAGATTCGTC ATGAGCAAAT CAGTGATTTG 
GATCGTCTCA AAGATCTTCC GTTTATTGTT TCGCATAGGT TGTCTAATAC CTTGAGCTTA 
GATATTCATG AAAATCATAG CCTTGCACTT CTAGGAAAGA AGAAATCAAG CAGTTTGACA 
TTACCACCCA AATATAATCA GCCATTCTTT GTTACTTCCT TACCTGATGA TAAAATACCT 
AAGGGGGCAG GACCCGGATG CTACCTTGCA GGCTCCTTGA CATTGTCAAA GACTGAGCTT 
GGAAAGAAAG CTGATGTGAT CCCTGTTCAT TACTATCTAA TACCTCCACC AACAAAGACT 
AAGAATGGCA GCAAAGATAA AGAAAAGGAT TCAGAAAAAG AGAAAGATTT GAAAGAAGAG 
TTTACTGAAG CTTTACGAGA TCTTAAAATT CAGTGGATGA CCAAGCTGGA TTCTACTGAC 
ATTTACAATG AATTGAAAGA AACATATCCT GCTTACCTTC CTTTGTATGT TGCACGTCTT 
CATCAATTAG ATGCTGAAAA GGAGCGGATG AAAAGACTTA ATGAAATTGT TGATGCTGCC 
AATGCTGTTA TTTCTCACAT CGATCAGACC GCTCTCGCTG TTTACATTGC CATGAAGACT 
GACCCCAGGC CTGATGCAGC TACTATAAAA AATGATATGG ACAAGCAGAA ATCTACCCTG 
GTAGATGCCC TCTGCAGGAA AGGATGTGCT CTGGCAGACC ACCTTCTTCA TGCACAGCCC 
CACGATGGGG CAGCAGCTGG AGATGCAGAA GCAAAAGAAG AGGAAGGAGA AAGTACCTTG 
GAATCTCTAT CAGAAACCTA TTGGGAAACT ACAAAGTGGA CAGATCTTTT TGACACTAAG 
GTTTTGACAT TTGCATACAA GCATGCATTA GTAAATAAGA TGTACGGGAG AGGCCTTAAG 
TTTGCAACCA AACTCGTAGA AGAAAAACCA ACAAAAGAAA ACTGGAAAAA TTGTATTCAA 
CTGATGAAAT TACTCGGATG GACCCATTGT GCGTCTTTTA CTGAAAACTG GCTCCCCATC 
ATGTATCCTC CTGATTATTG TGTATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_112399.1
CDS	14..3763
Translation	MATAATEEPFPFHGLLPKKETGASSFLCRYPEYDGRGVLIAVLDTGVDPGAPGMQVTTDGKPKIIDIIDTTGSGDVNTATEVEPKDGEITGLSGRVLKIPANWTNPSGKYHIGIKNGYDFYPKALKERIQKERKEKIWDPIHRVALAEACRKQEEFDIANNGSSQANKLIKEELQSQVELLNSFEKKYSDPGPVYDCLVWHDGETWRACVDSNENGDLGKSTVLRNYKEAQEYGSFGTAEMLNYSVNIYDDGNLLSIVTSGGAHGTHVASIAAGHFPEEPERNGVAPGAQILSIKIGDTRLSTMETGTGLIRAMIEVINHKCDLVNYSYGEATHWPNSGRICEVINEAVWKHNTIYVSSAGNNGPCLSTVGCPGGTTSSVIGVGAYVSPDMMVAEYSLREKLPANQYTWSSRGPSADGALGVSISAPGGAIASVPNWTLRGTQLMNGTSMSSPNACGGIALVLSGLKANNVDYTVHSVRRALENTAIKADNIEVFAQGHGIIQVDKAYDYLIQNTSFANRLGFTVTVGNNRGIYLRDPVQVAAPSDHGVGIEPVFPENTENSEKISFQLHLALTSNSSWVQCPSHLELMNQCRHINIRVDPRGLREGLHYTEVCGYDIASPNAGPLFRVPITAVIAAKVNESSHYDLAFTDVHFKPGQIRRHFVEVPEGATWAEVTVCSCSSEVSAKFVLHAVQLVKQRAYRSHEFYKFCSLPEKGTLIEAFPVLGGKAIEFCIARWWASLSDVNIDYTISFHGIVCTAPQLNIHASEGINRFDVQSSLKYEDLAPCITLKSWVQTLRPVNAKTRPLGSRDVLPNNRQLYEMVLTYSFHQPKSGEVTPSCPLLCELLYESEFDSQLWIIFDQNKRQMGSGDAYPHQYSLKLEKGDYTIRLQIRHEQISDLDRLKDLPFIVSHRLSNTLSLDIHENHSLALLGKKKSSSLTLPPKYNQPFFVTSLPDDKIPKGAGPGCYLAGSLTLSKTELGKKADVIPVHYYLIPPPTKTKNGSKDKEKDSEKEKDLKEEFTEALRDLKIQWMTKLDSTDIYNELKETYPAYLPLYVARLHQLDAEKERMKRLNEIVDAANAVISHIDQTALAVYIAMKTDPRPDAATIKNDMDKQKSTLVDALCRKGCALADHLLHAQPHDGAAAGDAEAKEEEGESTLESLSETYWETTKWTDLFDTKVLTFAYKHALVNKMYGRGLKFATKLVEEKPTKENWKNCIQLMKLLGWTHCASFTENWLPIMYPPDYCVF

Target ORF information:

RefSeq Version	NM_031137.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus tripeptidyl peptidase 2 (Tpp2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031137.1

ORF Insert Sequence:

ATGGCCACCG CTGCGACCGA GGAGCCTTTC CCTTTCCATG GTCTTCTACC AAAGAAAGAG 
ACTGGGGCCT CCTCCTTCCT GTGCCGCTAC CCGGAGTATG ACGGACGCGG GGTGCTCATC 
GCCGTCCTGG ACACAGGGGT TGATCCCGGG GCCCCGGGCA TGCAGGTCAC GACTGATGGG 
AAACCAAAAA TCATTGATAT CATTGATACA ACAGGAAGTG GTGATGTGAA TACTGCTACA 
GAAGTAGAAC CAAAAGATGG CGAAATCACT GGTCTTTCTG GAAGAGTGCT TAAGATTCCT 
GCAAACTGGA CAAATCCTTC AGGAAAATAT CATATTGGCA TTAAAAATGG TTATGACTTC 
TATCCAAAGG CTCTCAAGGA AAGGATACAG AAAGAACGGA AGGAAAAAAT CTGGGATCCA 
ATTCACAGAG TTGCGCTTGC AGAAGCTTGT AGAAAACAAG AAGAATTTGA TATTGCCAAT 
AATGGCTCTT CCCAAGCCAA TAAACTAATC AAGGAAGAGT TGCAGAGTCA AGTGGAATTA 
CTTAATTCTT TTGAGAAAAA GTATAGTGAT CCAGGCCCTG TGTATGACTG CTTGGTGTGG 
CATGATGGTG AGACCTGGAG AGCCTGTGTT GATTCGAATG AAAATGGGGA CTTGGGTAAA 
TCTACTGTAT TGAGAAACTA CAAAGAGGCT CAAGAATATG GTTCTTTTGG CACAGCTGAG 
ATGCTGAATT ACTCTGTTAA TATTTATGAT GATGGAAACC TGCTCTCCAT TGTGACCAGT 
GGAGGAGCTC ATGGAACCCA TGTAGCGAGT ATAGCTGCTG GGCATTTTCC AGAAGAGCCT 
GAACGGAATG GAGTTGCTCC TGGTGCTCAA ATTCTATCCA TTAAGATTGG TGATACAAGG 
CTAAGCACCA TGGAAACAGG CACAGGCCTC ATCAGAGCTA TGATAGAAGT TATAAATCAT 
AAGTGTGATC TTGTCAACTA CAGTTATGGA GAAGCAACTC ATTGGCCAAA TTCTGGGAGA 
ATTTGTGAAG TAATTAATGA AGCAGTATGG AAACATAATA CAATTTATGT TTCAAGTGCT 
GGAAATAATG GTCCATGCCT TTCTACAGTG GGTTGTCCAG GAGGAACTAC TTCCAGCGTG 
ATAGGTGTTG GAGCTTATGT TTCTCCTGAT ATGATGGTTG CAGAGTATTC ACTGAGAGAG 
AAACTACCTG CAAATCAATA TACATGGTCT TCTAGAGGCC CAAGTGCTGA TGGGGCCCTT 
GGTGTGAGCA TCAGTGCACC AGGAGGTGCT ATTGCTTCTG TGCCTAACTG GACATTGAGG 
GGGACTCAGC TAATGAATGG GACATCAATG TCTTCCCCCA ATGCCTGTGG TGGCATTGCC 
CTGGTACTTT CAGGGCTGAA AGCAAATAAT GTTGACTATA CTGTTCACTC AGTCAGAAGA 
GCTCTAGAAA ACACTGCAAT AAAAGCTGAC AATATAGAAG TATTTGCCCA AGGACATGGA 
ATTATTCAGG TTGACAAAGC TTATGACTAC CTCATTCAGA ATACATCATT TGCTAACAGA 
TTAGGTTTTA CAGTTACAGT TGGAAATAAC CGTGGCATCT ACCTCCGAGA TCCTGTTCAG 
GTGGCTGCTC CTTCAGATCA TGGTGTTGGC ATTGAGCCTG TATTTCCAGA GAACACAGAA 
AACTCTGAAA AAATATCCTT CCAGCTTCAT TTAGCTTTAA CTTCAAATTC ATCTTGGGTT 
CAATGTCCCA GCCATTTGGA ACTCATGAAT CAATGTCGGC ACATAAACAT ACGTGTGGAC 
CCCAGGGGCT TAAGAGAAGG GTTACATTAT ACAGAGGTAT GTGGGTATGA TATAGCATCC 
CCCAATGCAG GTCCTTTATT CAGAGTTCCA ATCACTGCAG TTATAGCAGC AAAAGTAAAT 
GAATCATCAC ATTATGATCT AGCCTTTACA GATGTACATT TTAAACCTGG TCAGATTCGA 
AGACATTTTG TTGAGGTTCC TGAGGGGGCA ACTTGGGCTG AAGTTACCGT GTGTTCGTGT 
TCTTCTGAGG TATCAGCAAA ATTTGTTCTT CATGCAGTAC AGCTTGTGAA GCAGAGAGCA 
TATCGAAGTC ATGAATTTTA TAAGTTTTGT TCCCTTCCAG AAAAAGGAAC ACTCATTGAA 
GCCTTTCCTG TTCTGGGTGG GAAAGCAATT GAATTTTGTA TTGCTCGTTG GTGGGCAAGT 
CTCAGTGATG TCAATATTGA TTATACCATA TCATTCCATG GCATAGTGTG TACTGCACCA 
CAGTTAAACA TTCATGCATC TGAAGGAATC AATCGTTTTG ATGTCCAGTC CTCTTTAAAA 
TATGAAGATC TGGCTCCTTG CATAACTTTG AAGAGCTGGG TGCAAACACT ACGCCCAGTA 
AATGCAAAAA CCAGACCTTT AGGATCAAGA GATGTTTTGC CAAATAATCG CCAGCTTTAT 
GAGATGGTCC TGACATACAG CTTTCATCAG CCCAAGAGCG GAGAAGTAAC ACCTAGTTGT 
CCACTCCTTT GTGAATTATT ATATGAGTCA GAATTTGACA GTCAGTTGTG GATTATTTTT 
GACCAGAACA AAAGACAGAT GGGCTCAGGC GATGCCTATC CACATCAGTA TTCTTTGAAA 
TTGGAGAAAG GAGATTATAC GATTCGATTA CAGATTCGTC ATGAGCAAAT CAGTGATTTG 
GATCGTCTCA AAGATCTTCC GTTTATTGTT TCGCATAGGT TGTCTAATAC CTTGAGCTTA 
GATATTCATG AAAATCATAG CCTTGCACTT CTAGGAAAGA AGAAATCAAG CAGTTTGACA 
TTACCACCCA AATATAATCA GCCATTCTTT GTTACTTCCT TACCTGATGA TAAAATACCT 
AAGGGGGCAG GACCCGGATG CTACCTTGCA GGCTCCTTGA CATTGTCAAA GACTGAGCTT 
GGAAAGAAAG CTGATGTGAT CCCTGTTCAT TACTATCTAA TACCTCCACC AACAAAGACT 
AAGAATGGCA GCAAAGATAA AGAAAAGGAT TCAGAAAAAG AGAAAGATTT GAAAGAAGAG 
TTTACTGAAG CTTTACGAGA TCTTAAAATT CAGTGGATGA CCAAGCTGGA TTCTACTGAC 
ATTTACAATG AATTGAAAGA AACATATCCT GCTTACCTTC CTTTGTATGT TGCACGTCTT 
CATCAATTAG ATGCTGAAAA GGAGCGGATG AAAAGACTTA ATGAAATTGT TGATGCTGCC 
AATGCTGTTA TTTCTCACAT CGATCAGACC GCTCTCGCTG TTTACATTGC CATGAAGACT 
GACCCCAGGC CTGATGCAGC TACTATAAAA AATGATATGG ACAAGCAGAA ATCTACCCTG 
GTAGATGCCC TCTGCAGGAA AGGATGTGCT CTGGCAGACC ACCTTCTTCA TGCACAGCCC 
CACGATGGGG CAGCAGCTGG AGATGCAGAA GCAAAAGAAG AGGAAGGAGA AAGTACCTTG 
GAATCTCTAT CAGAAACCTA TTGGGAAACT ACAAAGTGGA CAGATCTTTT TGACACTAAG 
GTTTTGACAT TTGCATACAA GCATGCATTA GTAAATAAGA TGTACGGGAG AGGCCTTAAG 
TTTGCAACCA AACTCGTAGA AGAAAAACCA ACAAAAGAAA ACTGGAAAAA TTGTATTCAA 
CTGATGAAAT TACTCGGATG GACCCATTGT GCGTCTTTTA CTGAAAACTG GCTCCCCATC 
ATGTATCCTC CTGATTATTG TGTATTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

[The changes in the activity of tripeptidyl peptidase II in experimental atherosclerosis and hypertension].
Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994)60(3)27-31(2014)
Pashevin DO,Honcharov SV,Tumanovs'ka LV,Dosenko VIe,Mo?benko OO

The structure of human tripeptidyl peptidase II as determined by a hybrid approach.
Structure (London, England : 1993)20(4)593-603(2012 Apr)
Sch?negge AM,Villa E,F?rster F,Hegerl R,Peters J,Baumeister W,Rockel B

Tripeptidyl-peptidase II expression and activity are increased in skeletal muscle during sepsis.
Biochemical and biophysical research communications296(1)41-7(2002 Aug)
Wray CJ,Tomkinson B,Robb BW,Hasselgren PO

Two new proteases in the MHC class I processing pathway.
Nature immunology1(5)413-8(2000 Nov)
Stoltze L,Schirle M,Schwarz G,Schr?ter C,Thompson MW,Hersh LB,Kalbacher H,Stevanovic S,Rammensee HG,Schild H

Interferon-gamma can stimulate post-proteasomal trimming of the N terminus of an antigenic peptide by inducing leucine aminopeptidase.
The Journal of biological chemistry273(30)18734-42(1998 Jul)
Beninga J,Rock KL,Goldberg AL

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Tpp2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

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