TppII cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	TppII
Entrez Gene ID	36444
Full Name	tripeptidyl-peptidase II
Synonyms	CG3991,Dmel\CG3991,TPP II,TPP2,TPPII,anon-WO0118547.127,dTPP II,dTPPII,l(2)k14301
General protein information	Preferred Names tripeptidyl-peptidase II Names CG3991 gene product from transcript CG3991-RD CG3991-PA CG3991-PD CG3991-PE CG3991-PF TppII-PA TppII-PD TppII-PE TppII-PF Tripeptidyl-peptidase II tripeptidyl peptidase II tripeptidylpeptidase II
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2R Map Location: 49F4-49F4

mRNA and Protein(s)

mRNA	Protein	Name
NM_001299447.1	NP_001286376.1	tripeptidyl-peptidase II, isoform F
NM_057899.3	NP_477247.1	tripeptidyl-peptidase II, isoform A
NM_001299448.1	NP_001286377.1	tripeptidyl-peptidase II, isoform E
NM_165966.2	NP_725252.1	tripeptidyl-peptidase II, isoform D

Pathways from BioSystems

Homologies

	TPP2	XP_002800874.1
Mus musculus (house mouse)	Tpp2	NP_033444.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004870	XP_314316.4
Oryza sativa (rice)	Os02g0664300	NP_001047661.1
Xenopus tropicalis (tropical clawed frog)	tpp2	XP_002933059.1
Bos taurus (cattle)	TPP2	NP_001092504.1
Danio rerio (zebrafish)	tpp2	XP_005168068.1
Schizosaccharomyces pombe (fission yeast)	tpp2	NP_594951.1
Arabidopsis thaliana (thale cress)	TPP2	NP_193817.2
Pan troglodytes (chimpanzee)	TPP2	XP_001151602.1
Rattus norvegicus (Norway rat)	Tpp2	NP_112399.1
Caenorhabditis elegans (roundworm)	F21H12.6	NP_495221.1
Homo sapiens (human)	TPP2	NP_003282.2
Canis lupus familiaris (dog)	TPP2	XP_005634119.1
Gallus gallus (chicken)	TPP2	XP_004938615.1
Drosophila melanogaster (fruit fly)	TppII	NP_725252.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Previously unknown differences between TPP II orthologues and subtilisin as well as features that might be conserved within the entire family of subtilisin-like serine peptidases.
Title: Exploring the active site of tripeptidyl-peptidase II through studies of pH dependence of reaction kinetics.

a systematic kinetic analysis of wild type Drosophila melanogaster TPPII and five point mutants of the double-Glu-motif (E312/E343) involving natural substrates were performed.
Title: Molecular ruler of tripeptidylpeptidase II: mechanistic principle of exopeptidase selectivity.

The structure suggests a model for activation of TPP II involving the relocation of a flexible loop and a repositioning of the active-site serine, coupling it to holocomplex assembly and active-site sequestration.
Title: Hybrid molecular structure of the giant protease tripeptidyl peptidase II.

the unique spindle form of the holo-complex of TPP II represents an assembly motif stabilizing a highly active state
Title: Size matters for the tripeptidylpeptidase II complex from Drosophila: The 6-MDa spindle form stabilizes the activated state.

The 150 kDa subunits of TPPII form a superstructure composed of 2 segmented & twisted strands. Each strand is 12.5 nm in width & composed of 11 segments that enclose a central channel.
Title: A giant protease with a twist: the TPP II complex from Drosophila studied by electron microscopy.

The following TppII gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TppII cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa12964	NM_165966.2 Latest version!	Drosophila melanogaster tripeptidyl-peptidase II (TppII), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa12965	NM_001299447.1 Latest version!	Drosophila melanogaster tripeptidyl-peptidase II (TppII), transcript variant F, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa12965	NM_057899.3 Latest version!	Drosophila melanogaster tripeptidyl-peptidase II (TppII), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa21796	NM_001299448.1 Latest version!	Drosophila melanogaster tripeptidyl-peptidase II (TppII), transcript variant E, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TppII ( NM_165966.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_725252.1 Drosophila melanogaster tripeptidyl-peptidase II (TppII), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa12964
Clone ID Related Accession (Same CDS sequence)	NM_165966.2
Accession Version	NM_165966.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4326bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	tripeptidyl-peptidase II, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_165966.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGTTTAACA GATTCCGATT GGTGCACAAA CAGCTGCGGT TATATAAGAA TTTCGGGCTG 
CTGGGCCAGA AGGCCAGCGT GGGACTCACA TTGCCGATCA TCAGCTTGTC CAGGCCGTAC 
ATGGCGTATA TGGGCACCGA GAGGAGCGTG GTGATGATCA CCGCACCAGC TACGAAGGAA 
TTCGCAGAGA GTTCTGAGCG CAGCAATAGC AGCAAGAAGA CTACCAACAA AGAGCAGAGC 
GACAAATCAG CGGAGAGCAG GATGGCCACC AGCGGTATTG TCGAGTCATT TCCCACGGGC 
GCACTGGTGC CCAAGGCGGA GACGGGAGTC CTGAACTTTC TGCAGAAGTA CCCGGAGTAC 
GATGGACGCG ACGTCACCAT AGCCATCTTC GATTCCGGCG TCGATCCCCG GGCAACGGGA 
CTGGAGACGC TGTGCGATGG AAAGACCGTT AAAGTAATAG AGCGGTACGA CTGTTCCGGA 
TGCGGCGACG TGGACATGAA GAAGAAGGTG ACGCCGGACG AGAACGGCAA CATTAAGGGC 
CTGTCCGGAA ACTCGCTCAA GCTGAGTCCG GAGCTGATGG CTCTGAACAC AGATCCGGAG 
AAGGCGGTGC GGGTAGGCCT CAAGAGCTTC AGCGATTTGT TGCCCTCCAA GGTGCGGAAC 
AACATTGTGG CCCAGGCCAA GCTGAAGCAC TGGGACAAGC CGCACAAGAC GGCCACTGCA 
AACGCCAGTC GCAAGATTGT TGAATTTGAG TCACAAAATC CAGGAGAAGC CTCAAAACTG 
CCCTGGGACA AGAAGATATT GAAAGAGAAT CTAGACTTTG AGCTAGAGAT GTTGAATAGC 
TACGAGAAGG TGTACGGCGA CATTAAGACC TCCTACGATT GCATCCTTTT TCCCACGGCC 
GACGGATGGC TGACAATCGT CGACACCACG GAGCAGGGCG ATCTGGATCA GGCTCTGCGC 
ATTGGCGAGT ATTCCCGCAC GCACGAGACC CGCAATGTGG ACGACTTTCT TTCCATATCG 
GTAAACGTCC ACGACGAGGG CAACGTACTG GAGGTGGTCG GCATGAGCTC ACCCCACGGC 
ACCCACGTAT CGTCTATTGC CAGCGGCAAC CATAGCTCCC GGGACGTGGA TGGCGTGGCG 
CCGAATGCTA AGATCGTTTC TATGACCATC GGTGATGGTC GACTTGGGTC TATGGAGACC 
GGCACGGCAC TAGTGCGTGC CATGACTAAG GTGATGGAAC TATGTCGCGA TGGCAGACGC 
ATCGATGTGA TCAACATGAG CTATGGCGAG CATGCCAATT GGTCAAATTC CGGCCGCATT 
GGGGAGCTCA TGAACGAGGT TGTCAACAAG TATGGCGTGG TGTGGGTGGC ATCGGCCGGC 
AACCATGGTC CGGCACTTTG TACTGTGGGA ACTCCGCCGG ACATCAGCCA GCCCAGTTTG 
ATCGGCGTGG GCGCGTACGT ATCACCCCAA ATGATGGAGG CCGAGTATGC GATGCGAGAA 
AAGCTGCCCG GGAACGTGTA CACCTGGACA TCGCGAGATC CCTGCATCGA CGGAGGTCAA 
GGCGTTACCG TATGCGCTCC GGGCGGAGCC ATTGCGTCCG TGCCACAGTT TACTATGAGC 
AAGTCCCAGC TGATGAACGG TACCAGCATG GCGGCACCTC ACGTCGCCGG CGCAGTGGCG 
CTGCTCATCT CCGGTCTGAA GCAACAGAAC ATCGAGTATT CGCCGTACAG TATTAAGCGA 
GCGATCAGCG TCACTGCCAC CAAACTGGGC TATGTAGATC CCTTTGCTCA GGGCCATGGC 
TTGCTCAATG TCGAGAAGGC ATTCGAGCAT TTGACGGAGC ACCGCCAGTC CAAGGATAAT 
ATGCTCAGGT TCTCCGTGCG CGTGGGCAAC AACGCAGACA AGGGCATTCA TTTGCGTCAG 
GGCGTGCAGC GTAACTCCAT CGATTACAAC GTTTATATAG AGCCCATCTT CTACAATGAC 
AAGGAGGCGG ATCCCAAGGA CAAGTTTAAC TTCAATGTAC GGCTAAATCT GATTGCCTCG 
CAGCCGTGGG TGCAGTGTGG AGCTTTCTTG GATCTCAGCT ATGGCACCCG CTCCATTGCC 
GTGCGCGTCG ATCCCACTGG ACTCCAGCCA GGCGTTCACA GCGCTGTGAT TCGGGCTTAT 
GACACTGACT GCGTACAGAA GGGTTCTCTC TTTGAGATTC CTGTCACGGT GGTGCAGCCC 
CATGTGCTGG AGTCGGATCA GAACACGCCC GTCTTCGAAC CCGCCTCTTC CAAGGGAGAC 
AACAGCGTGG AGTTTCAGCC AAACACCATT CAAAGAGACT TCATCCTGGT GCCAGAACGT 
GCTACTTGGG CGGAGTTGCG TATGCGTATA ACCGATCCCA ATCGTGGCGA GGACATTGGA 
AAGTTCTTTG TACACACGAA CCAACTTCTT CCCAAGCAAT CCTGTCGTAA GCTTGAGACC 
ATGAAGATCG TATCGGTTGG CTCGGAAAAC GAATCTATAA TGGCTTTTAA AGTTAAGTCT 
GGCAGGATTC TGGAGCTATG CATTGCCAAG TACTGGTCCA ACTACGGCCA GAGTCACCTG 
AAGTACAGCT TGCGTTTCCG TGGCGTGGAA GCGCACAATC CCAATGCCTA CGTCATGCAT 
GCGGGCAGGG GAATTCATAA GCTGGAGATT GAGGCCCTGG TTGCCGAGGA TGTGCAGCCC 
CAGCTACAGC TAAAGAATGC CGAAGTGGTG CTAAAACCGA CCGAGGCCAA GATCTCGCCG 
CTAAGCGCCA CACGAGACGT CATCCCAGAT GGACGTCAGG TGTATCAGAA CCTGCTGGCC 
TTTAACTTGA ACGTGGCCAA GGCCGCAGAT GTGTCGATAT ACGCACCGAT CTTTAACGAC 
TTGTTGTATG AAGCGGAGTT TGAGTCACAG ATGTGGATGC TTTTTGATGC AAACAAGGCC 
CTGGTGGCCA CCGGCGATGC TCACTCCCAC ACTTCCTTCA CAAAGCTCGA TAAGGGCGAA 
TACACAATCA GACTGCAGGT GCGTCACGAG AAGCGCGACC TGCTGGAGAA GATCTCGGAG 
GCAAATCTGG TGGCCTCATT CAAGCTGACT AGCCCCCTCA CCCTTGATTT CTATGAGAAC 
TACAATCAGT GCATAGTGGG AGGTCGTAAA TACGTCTCGA GTCCGCTAAG GCTGTCCACT 
CGGGTGCTTT ATATCGCTCC TATTACCCAA GAGCGACTTA CCAAGGCCAA TCTGCCCGCT 
CAATGCGCCT GGCTGAGCGG CAATCTGGTA TTCCCGCAAG ATGAGGTCGG ACGGCGAGTG 
GCTCAGCATC CATTTACTTA CATCCTCAAT CCCGCTGAGA AAAAGTCACA CACGAATGGC 
TCGAGCAACG GTTCCAGCGC TGCAGGATCC ACAGCTACGG CAGCTGCTGT CACCACTGCT 
AATGGCGCAA AACCGAAAGC TCCGGCGACT CCACAAGCGG CCACCTCAGT GACCAATCCT 
GCGGCCGGCG ATGGAATTTC TGTTCAGAAC GACCCGCCTG TGGACAGCAG TGGGAGTCCC 
GCCTCACCCA AAAAGGGCAA GGCCAATGCC GACGATTATG CCGAAAGTTT TCGCGACTTT 
CAATGTTCGC AGATTGTCAA GTGTGAACTG GAAATGGCAG AGAAAATCTA TAATGATGTA 
GTCGCTGCCC ATCCCAAGCA TTTGCAGGCA AACCTGCTAC TTATCCAGAA CATCGAGTCC 
AATCAGCTGA AGTCGCAACT GCCGCTGACT TTCGTCAATG CTCAAAAGAC ATCGCCACCA 
GAGGCAGGCG AGAGCGCCGA CAAGCAAAAG GAGGATCAAA AGAAAGTACG AAGCGCCCTG 
GAGCGAATTG TTAAGCTGGC CGACAAGGTG ATCCAGGAGA CCGATTCCGA GGCACTGCTC 
TCCTACTACG GTCTGAAGAA CGACACTCGT GCCGATGCAG CCAAGATAAA GACCAACATG 
GACAAACAGA AAAACACTCT CATTGAGGCG CTAAGCAAGA AGGGCATTGC TGTGGCAAAG 
CTGGCTGTAT TAGACGACTG TATCAAGGAT AGCCTGGCTG AGATCAACGA GCTGTATACC 
GAGATTATCA AGTTCGTGGA CGCCAACGAC TCCAAGGCCA TCCAGTTCGC TCTGTGGCAC 
GCCTATGCCC ATGGCCACTA CGGTCGCATG TACAAGTATG TGGTTAAGCT AATCGAGGAG 
AAGCGAACCC GCGATCACTT CGTGGAGCTG GCCGCCATCA ACGGCGCCCT GGGTCACGAG 
CACATCCGTA CTGTCATCAA CCGTATGATG ATCACTGCCT TTCCCAGCAG CTTCCGTTTG 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_725252.1
CDS	207..4532
Translation	MFNRFRLVHKQLRLYKNFGLLGQKASVGLTLPIISLSRPYMAYMGTERSVVMITAPATKEFAESSERSNSSKKTTNKEQSDKSAESRMATSGIVESFPTGALVPKAETGVLNFLQKYPEYDGRDVTIAIFDSGVDPRATGLETLCDGKTVKVIERYDCSGCGDVDMKKKVTPDENGNIKGLSGNSLKLSPELMALNTDPEKAVRVGLKSFSDLLPSKVRNNIVAQAKLKHWDKPHKTATANASRKIVEFESQNPGEASKLPWDKKILKENLDFELEMLNSYEKVYGDIKTSYDCILFPTADGWLTIVDTTEQGDLDQALRIGEYSRTHETRNVDDFLSISVNVHDEGNVLEVVGMSSPHGTHVSSIASGNHSSRDVDGVAPNAKIVSMTIGDGRLGSMETGTALVRAMTKVMELCRDGRRIDVINMSYGEHANWSNSGRIGELMNEVVNKYGVVWVASAGNHGPALCTVGTPPDISQPSLIGVGAYVSPQMMEAEYAMREKLPGNVYTWTSRDPCIDGGQGVTVCAPGGAIASVPQFTMSKSQLMNGTSMAAPHVAGAVALLISGLKQQNIEYSPYSIKRAISVTATKLGYVDPFAQGHGLLNVEKAFEHLTEHRQSKDNMLRFSVRVGNNADKGIHLRQGVQRNSIDYNVYIEPIFYNDKEADPKDKFNFNVRLNLIASQPWVQCGAFLDLSYGTRSIAVRVDPTGLQPGVHSAVIRAYDTDCVQKGSLFEIPVTVVQPHVLESDQNTPVFEPASSKGDNSVEFQPNTIQRDFILVPERATWAELRMRITDPNRGEDIGKFFVHTNQLLPKQSCRKLETMKIVSVGSENESIMAFKVKSGRILELCIAKYWSNYGQSHLKYSLRFRGVEAHNPNAYVMHAGRGIHKLEIEALVAEDVQPQLQLKNAEVVLKPTEAKISPLSATRDVIPDGRQVYQNLLAFNLNVAKAADVSIYAPIFNDLLYEAEFESQMWMLFDANKALVATGDAHSHTSFTKLDKGEYTIRLQVRHEKRDLLEKISEANLVASFKLTSPLTLDFYENYNQCIVGGRKYVSSPLRLSTRVLYIAPITQERLTKANLPAQCAWLSGNLVFPQDEVGRRVAQHPFTYILNPAEKKSHTNGSSNGSSAAGSTATAAAVTTANGAKPKAPATPQAATSVTNPAAGDGISVQNDPPVDSSGSPASPKKGKANADDYAESFRDFQCSQIVKCELEMAEKIYNDVVAAHPKHLQANLLLIQNIESNQLKSQLPLTFVNAQKTSPPEAGESADKQKEDQKKVRSALERIVKLADKVIQETDSEALLSYYGLKNDTRADAAKIKTNMDKQKNTLIEALSKKGIAVAKLAVLDDCIKDSLAEINELYTEIIKFVDANDSKAIQFALWHAYAHGHYGRMYKYVVKLIEEKRTRDHFVELAAINGALGHEHIRTVINRMMITAFPSSFRLF

Target ORF information:

RefSeq Version	NM_165966.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster tripeptidyl-peptidase II (TppII), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_165966.2

ORF Insert Sequence:

ATGTTTAACA GATTCCGATT GGTGCACAAA CAGCTGCGGT TATATAAGAA TTTCGGGCTG 
CTGGGCCAGA AGGCCAGCGT GGGACTCACA TTGCCGATCA TCAGCTTGTC CAGGCCGTAC 
ATGGCGTATA TGGGCACCGA GAGGAGCGTG GTGATGATCA CCGCACCAGC TACGAAGGAA 
TTCGCAGAGA GTTCTGAGCG CAGCAATAGC AGCAAGAAGA CTACCAACAA AGAGCAGAGC 
GACAAATCAG CGGAGAGCAG GATGGCCACC AGCGGTATTG TCGAGTCATT TCCCACGGGC 
GCACTGGTGC CCAAGGCGGA GACGGGAGTC CTGAACTTTC TGCAGAAGTA CCCGGAGTAC 
GATGGACGCG ACGTCACCAT AGCCATCTTC GATTCCGGCG TCGATCCCCG GGCAACGGGA 
CTGGAGACGC TGTGCGATGG AAAGACCGTT AAAGTAATAG AGCGGTACGA CTGTTCCGGA 
TGCGGCGACG TGGACATGAA GAAGAAGGTG ACGCCGGACG AGAACGGCAA CATTAAGGGC 
CTGTCCGGAA ACTCGCTCAA GCTGAGTCCG GAGCTGATGG CTCTGAACAC AGATCCGGAG 
AAGGCGGTGC GGGTAGGCCT CAAGAGCTTC AGCGATTTGT TGCCCTCCAA GGTGCGGAAC 
AACATTGTGG CCCAGGCCAA GCTGAAGCAC TGGGACAAGC CGCACAAGAC GGCCACTGCA 
AACGCCAGTC GCAAGATTGT TGAATTTGAG TCACAAAATC CAGGAGAAGC CTCAAAACTG 
CCCTGGGACA AGAAGATATT GAAAGAGAAT CTAGACTTTG AGCTAGAGAT GTTGAATAGC 
TACGAGAAGG TGTACGGCGA CATTAAGACC TCCTACGATT GCATCCTTTT TCCCACGGCC 
GACGGATGGC TGACAATCGT CGACACCACG GAGCAGGGCG ATCTGGATCA GGCTCTGCGC 
ATTGGCGAGT ATTCCCGCAC GCACGAGACC CGCAATGTGG ACGACTTTCT TTCCATATCG 
GTAAACGTCC ACGACGAGGG CAACGTACTG GAGGTGGTCG GCATGAGCTC ACCCCACGGC 
ACCCACGTAT CGTCTATTGC CAGCGGCAAC CATAGCTCCC GGGACGTGGA TGGCGTGGCG 
CCGAATGCTA AGATCGTTTC TATGACCATC GGTGATGGTC GACTTGGGTC TATGGAGACC 
GGCACGGCAC TAGTGCGTGC CATGACTAAG GTGATGGAAC TATGTCGCGA TGGCAGACGC 
ATCGATGTGA TCAACATGAG CTATGGCGAG CATGCCAATT GGTCAAATTC CGGCCGCATT 
GGGGAGCTCA TGAACGAGGT TGTCAACAAG TATGGCGTGG TGTGGGTGGC ATCGGCCGGC 
AACCATGGTC CGGCACTTTG TACTGTGGGA ACTCCGCCGG ACATCAGCCA GCCCAGTTTG 
ATCGGCGTGG GCGCGTACGT ATCACCCCAA ATGATGGAGG CCGAGTATGC GATGCGAGAA 
AAGCTGCCCG GGAACGTGTA CACCTGGACA TCGCGAGATC CCTGCATCGA CGGAGGTCAA 
GGCGTTACCG TATGCGCTCC GGGCGGAGCC ATTGCGTCCG TGCCACAGTT TACTATGAGC 
AAGTCCCAGC TGATGAACGG TACCAGCATG GCGGCACCTC ACGTCGCCGG CGCAGTGGCG 
CTGCTCATCT CCGGTCTGAA GCAACAGAAC ATCGAGTATT CGCCGTACAG TATTAAGCGA 
GCGATCAGCG TCACTGCCAC CAAACTGGGC TATGTAGATC CCTTTGCTCA GGGCCATGGC 
TTGCTCAATG TCGAGAAGGC ATTCGAGCAT TTGACGGAGC ACCGCCAGTC CAAGGATAAT 
ATGCTCAGGT TCTCCGTGCG CGTGGGCAAC AACGCAGACA AGGGCATTCA TTTGCGTCAG 
GGCGTGCAGC GTAACTCCAT CGATTACAAC GTTTATATAG AGCCCATCTT CTACAATGAC 
AAGGAGGCGG ATCCCAAGGA CAAGTTTAAC TTCAATGTAC GGCTAAATCT GATTGCCTCG 
CAGCCGTGGG TGCAGTGTGG AGCTTTCTTG GATCTCAGCT ATGGCACCCG CTCCATTGCC 
GTGCGCGTCG ATCCCACTGG ACTCCAGCCA GGCGTTCACA GCGCTGTGAT TCGGGCTTAT 
GACACTGACT GCGTACAGAA GGGTTCTCTC TTTGAGATTC CTGTCACGGT GGTGCAGCCC 
CATGTGCTGG AGTCGGATCA GAACACGCCC GTCTTCGAAC CCGCCTCTTC CAAGGGAGAC 
AACAGCGTGG AGTTTCAGCC AAACACCATT CAAAGAGACT TCATCCTGGT GCCAGAACGT 
GCTACTTGGG CGGAGTTGCG TATGCGTATA ACCGATCCCA ATCGTGGCGA GGACATTGGA 
AAGTTCTTTG TACACACGAA CCAACTTCTT CCCAAGCAAT CCTGTCGTAA GCTTGAGACC 
ATGAAGATCG TATCGGTTGG CTCGGAAAAC GAATCTATAA TGGCTTTTAA AGTTAAGTCT 
GGCAGGATTC TGGAGCTATG CATTGCCAAG TACTGGTCCA ACTACGGCCA GAGTCACCTG 
AAGTACAGCT TGCGTTTCCG TGGCGTGGAA GCGCACAATC CCAATGCCTA CGTCATGCAT 
GCGGGCAGGG GAATTCATAA GCTGGAGATT GAGGCCCTGG TTGCCGAGGA TGTGCAGCCC 
CAGCTACAGC TAAAGAATGC CGAAGTGGTG CTAAAACCGA CCGAGGCCAA GATCTCGCCG 
CTAAGCGCCA CACGAGACGT CATCCCAGAT GGACGTCAGG TGTATCAGAA CCTGCTGGCC 
TTTAACTTGA ACGTGGCCAA GGCCGCAGAT GTGTCGATAT ACGCACCGAT CTTTAACGAC 
TTGTTGTATG AAGCGGAGTT TGAGTCACAG ATGTGGATGC TTTTTGATGC AAACAAGGCC 
CTGGTGGCCA CCGGCGATGC TCACTCCCAC ACTTCCTTCA CAAAGCTCGA TAAGGGCGAA 
TACACAATCA GACTGCAGGT GCGTCACGAG AAGCGCGACC TGCTGGAGAA GATCTCGGAG 
GCAAATCTGG TGGCCTCATT CAAGCTGACT AGCCCCCTCA CCCTTGATTT CTATGAGAAC 
TACAATCAGT GCATAGTGGG AGGTCGTAAA TACGTCTCGA GTCCGCTAAG GCTGTCCACT 
CGGGTGCTTT ATATCGCTCC TATTACCCAA GAGCGACTTA CCAAGGCCAA TCTGCCCGCT 
CAATGCGCCT GGCTGAGCGG CAATCTGGTA TTCCCGCAAG ATGAGGTCGG ACGGCGAGTG 
GCTCAGCATC CATTTACTTA CATCCTCAAT CCCGCTGAGA AAAAGTCACA CACGAATGGC 
TCGAGCAACG GTTCCAGCGC TGCAGGATCC ACAGCTACGG CAGCTGCTGT CACCACTGCT 
AATGGCGCAA AACCGAAAGC TCCGGCGACT CCACAAGCGG CCACCTCAGT GACCAATCCT 
GCGGCCGGCG ATGGAATTTC TGTTCAGAAC GACCCGCCTG TGGACAGCAG TGGGAGTCCC 
GCCTCACCCA AAAAGGGCAA GGCCAATGCC GACGATTATG CCGAAAGTTT TCGCGACTTT 
CAATGTTCGC AGATTGTCAA GTGTGAACTG GAAATGGCAG AGAAAATCTA TAATGATGTA 
GTCGCTGCCC ATCCCAAGCA TTTGCAGGCA AACCTGCTAC TTATCCAGAA CATCGAGTCC 
AATCAGCTGA AGTCGCAACT GCCGCTGACT TTCGTCAATG CTCAAAAGAC ATCGCCACCA 
GAGGCAGGCG AGAGCGCCGA CAAGCAAAAG GAGGATCAAA AGAAAGTACG AAGCGCCCTG 
GAGCGAATTG TTAAGCTGGC CGACAAGGTG ATCCAGGAGA CCGATTCCGA GGCACTGCTC 
TCCTACTACG GTCTGAAGAA CGACACTCGT GCCGATGCAG CCAAGATAAA GACCAACATG 
GACAAACAGA AAAACACTCT CATTGAGGCG CTAAGCAAGA AGGGCATTGC TGTGGCAAAG 
CTGGCTGTAT TAGACGACTG TATCAAGGAT AGCCTGGCTG AGATCAACGA GCTGTATACC 
GAGATTATCA AGTTCGTGGA CGCCAACGAC TCCAAGGCCA TCCAGTTCGC TCTGTGGCAC 
GCCTATGCCC ATGGCCACTA CGGTCGCATG TACAAGTATG TGGTTAAGCT AATCGAGGAG 
AAGCGAACCC GCGATCACTT CGTGGAGCTG GCCGCCATCA ACGGCGCCCT GGGTCACGAG 
CACATCCGTA CTGTCATCAA CCGTATGATG ATCACTGCCT TTCCCAGCAG CTTCCGTTTG 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TppII ( NM_001299447.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286376.1 Drosophila melanogaster tripeptidyl-peptidase II (TppII), transcript variant F, mRNA.

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CloneID	OFa12965
Clone ID Related Accession (Same CDS sequence)	NM_001299447.1 , NM_057899.3
Accession Version	NM_001299447.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	tripeptidyl-peptidase II, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCACCA GCGGTATTGT CGAGTCATTT CCCACGGGCG CACTGGTGCC CAAGGCGGAG 
ACGGGAGTCC TGAACTTTCT GCAGAAGTAC CCGGAGTACG ATGGACGCGA CGTCACCATA 
GCCATCTTCG ATTCCGGCGT CGATCCCCGG GCAACGGGAC TGGAGACGCT GTGCGATGGA 
AAGACCGTTA AAGTAATAGA GCGGTACGAC TGTTCCGGAT GCGGCGACGT GGACATGAAG 
AAGAAGGTGA CGCCGGACGA GAACGGCAAC ATTAAGGGCC TGTCCGGAAA CTCGCTCAAG 
CTGAGTCCGG AGCTGATGGC TCTGAACACA GATCCGGAGA AGGCGGTGCG GGTAGGCCTC 
AAGAGCTTCA GCGATTTGTT GCCCTCCAAG GTGCGGAACA ACATTGTGGC CCAGGCCAAG 
CTGAAGCACT GGGACAAGCC GCACAAGACG GCCACTGCAA ACGCCAGTCG CAAGATTGTT 
GAATTTGAGT CACAAAATCC AGGAGAAGCC TCAAAACTGC CCTGGGACAA GAAGATATTG 
AAAGAGAATC TAGACTTTGA GCTAGAGATG TTGAATAGCT ACGAGAAGGT GTACGGCGAC 
ATTAAGACCT CCTACGATTG CATCCTTTTT CCCACGGCCG ACGGATGGCT GACAATCGTC 
GACACCACGG AGCAGGGCGA TCTGGATCAG GCTCTGCGCA TTGGCGAGTA TTCCCGCACG 
CACGAGACCC GCAATGTGGA CGACTTTCTT TCCATATCGG TAAACGTCCA CGACGAGGGC 
AACGTACTGG AGGTGGTCGG CATGAGCTCA CCCCACGGCA CCCACGTATC GTCTATTGCC 
AGCGGCAACC ATAGCTCCCG GGACGTGGAT GGCGTGGCGC CGAATGCTAA GATCGTTTCT 
ATGACCATCG GTGATGGTCG ACTTGGGTCT ATGGAGACCG GCACGGCACT AGTGCGTGCC 
ATGACTAAGG TGATGGAACT ATGTCGCGAT GGCAGACGCA TCGATGTGAT CAACATGAGC 
TATGGCGAGC ATGCCAATTG GTCAAATTCC GGCCGCATTG GGGAGCTCAT GAACGAGGTT 
GTCAACAAGT ATGGCGTGGT GTGGGTGGCA TCGGCCGGCA ACCATGGTCC GGCACTTTGT 
ACTGTGGGAA CTCCGCCGGA CATCAGCCAG CCCAGTTTGA TCGGCGTGGG CGCGTACGTA 
TCACCCCAAA TGATGGAGGC CGAGTATGCG ATGCGAGAAA AGCTGCCCGG GAACGTGTAC 
ACCTGGACAT CGCGAGATCC CTGCATCGAC GGAGGTCAAG GCGTTACCGT ATGCGCTCCG 
GGCGGAGCCA TTGCGTCCGT GCCACAGTTT ACTATGAGCA AGTCCCAGCT GATGAACGGT 
ACCAGCATGG CGGCACCTCA CGTCGCCGGC GCAGTGGCGC TGCTCATCTC CGGTCTGAAG 
CAACAGAACA TCGAGTATTC GCCGTACAGT ATTAAGCGAG CGATCAGCGT CACTGCCACC 
AAACTGGGCT ATGTAGATCC CTTTGCTCAG GGCCATGGCT TGCTCAATGT CGAGAAGGCA 
TTCGAGCATT TGACGGAGCA CCGCCAGTCC AAGGATAATA TGCTCAGGTT CTCCGTGCGC 
GTGGGCAACA ACGCAGACAA GGGCATTCAT TTGCGTCAGG GCGTGCAGCG TAACTCCATC 
GATTACAACG TTTATATAGA GCCCATCTTC TACAATGACA AGGAGGCGGA TCCCAAGGAC 
AAGTTTAACT TCAATGTACG GCTAAATCTG ATTGCCTCGC AGCCGTGGGT GCAGTGTGGA 
GCTTTCTTGG ATCTCAGCTA TGGCACCCGC TCCATTGCCG TGCGCGTCGA TCCCACTGGA 
CTCCAGCCAG GCGTTCACAG CGCTGTGATT CGGGCTTATG ACACTGACTG CGTACAGAAG 
GGTTCTCTCT TTGAGATTCC TGTCACGGTG GTGCAGCCCC ATGTGCTGGA GTCGGATCAG 
AACACGCCCG TCTTCGAACC CGCCTCTTCC AAGGGAGACA ACAGCGTGGA GTTTCAGCCA 
AACACCATTC AAAGAGACTT CATCCTGGTG CCAGAACGTG CTACTTGGGC GGAGTTGCGT 
ATGCGTATAA CCGATCCCAA TCGTGGCGAG GACATTGGAA AGTTCTTTGT ACACACGAAC 
CAACTTCTTC CCAAGCAATC CTGTCGTAAG CTTGAGACCA TGAAGATCGT ATCGGTTGGC 
TCGGAAAACG AATCTATAAT GGCTTTTAAA GTTAAGTCTG GCAGGATTCT GGAGCTATGC 
ATTGCCAAGT ACTGGTCCAA CTACGGCCAG AGTCACCTGA AGTACAGCTT GCGTTTCCGT 
GGCGTGGAAG CGCACAATCC CAATGCCTAC GTCATGCATG CGGGCAGGGG AATTCATAAG 
CTGGAGATTG AGGCCCTGGT TGCCGAGGAT GTGCAGCCCC AGCTACAGCT AAAGAATGCC 
GAAGTGGTGC TAAAACCGAC CGAGGCCAAG ATCTCGCCGC TAAGCGCCAC ACGAGACGTC 
ATCCCAGATG GACGTCAGGT GTATCAGAAC CTGCTGGCCT TTAACTTGAA CGTGGCCAAG 
GCCGCAGATG TGTCGATATA CGCACCGATC TTTAACGACT TGTTGTATGA AGCGGAGTTT 
GAGTCACAGA TGTGGATGCT TTTTGATGCA AACAAGGCCC TGGTGGCCAC CGGCGATGCT 
CACTCCCACA CTTCCTTCAC AAAGCTCGAT AAGGGCGAAT ACACAATCAG ACTGCAGGTG 
CGTCACGAGA AGCGCGACCT GCTGGAGAAG ATCTCGGAGG CAAATCTGGT GGCCTCATTC 
AAGCTGACTA GCCCCCTCAC CCTTGATTTC TATGAGAACT ACAATCAGTG CATAGTGGGA 
GGTCGTAAAT ACGTCTCGAG TCCGCTAAGG CTGTCCACTC GGGTGCTTTA TATCGCTCCT 
ATTACCCAAG AGCGACTTAC CAAGGCCAAT CTGCCCGCTC AATGCGCCTG GCTGAGCGGC 
AATCTGGTAT TCCCGCAAGA TGAGGTCGGA CGGCGAGTGG CTCAGCATCC ATTTACTTAC 
ATCCTCAATC CCGCTGAGAA AAAGTCACAC ACGAATGGCT CGAGCAACGG TTCCAGCGCT 
GCAGGATCCA CAGCTACGGC AGCTGCTGTC ACCACTGCTA ATGGCGCAAA ACCGAAAGCT 
CCGGCGACTC CACAAGCGGC CACCTCAGTG ACCAATCCTG CGGCCGGCGA TGGAATTTCT 
GTTCAGAACG ACCCGCCTGT GGACAGCAGT GGGAGTCCCG CCTCACCCAA AAAGGGCAAG 
GCCAATGCCG ACGATTATGC CGAAAGTTTT CGCGACTTTC AATGTTCGCA GATTGTCAAG 
TGTGAACTGG AAATGGCAGA GAAAATCTAT AATGATGTAG TCGCTGCCCA TCCCAAGCAT 
TTGCAGGCAA ACCTGCTACT TATCCAGAAC ATCGAGTCCA ATCAGCTGAA GTCGCAACTG 
CCGCTGACTT TCGTCAATGC TCAAAAGACA TCGCCACCAG AGGCAGGCGA GAGCGCCGAC 
AAGCAAAAGG AGGATCAAAA GAAAGTACGA AGCGCCCTGG AGCGAATTGT TAAGCTGGCC 
GACAAGGTGA TCCAGGAGAC CGATTCCGAG GCACTGCTCT CCTACTACGG TCTGAAGAAC 
GACACTCGTG CCGATGCAGC CAAGATAAAG ACCAACATGG ACAAACAGAA AAACACTCTC 
ATTGAGGCGC TAAGCAAGAA GGGCATTGCT GTGGCAAAGC TGGCTGTATT AGACGACTGT 
ATCAAGGATA GCCTGGCTGA GATCAACGAG CTGTATACCG AGATTATCAA GTTCGTGGAC 
GCCAACGACT CCAAGGCCAT CCAGTTCGCT CTGTGGCACG CCTATGCCCA TGGCCACTAC 
GGTCGCATGT ACAAGTATGT GGTTAAGCTA ATCGAGGAGA AGCGAACCCG CGATCACTTC 
GTGGAGCTGG CCGCCATCAA CGGCGCCCTG GGTCACGAGC ACATCCGTAC TGTCATCAAC 
CGTATGATGA TCACTGCCTT TCCCAGCAGC TTCCGTTTGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286376.1
CDS	202..4266
Translation	MATSGIVESFPTGALVPKAETGVLNFLQKYPEYDGRDVTIAIFDSGVDPRATGLETLCDGKTVKVIERYDCSGCGDVDMKKKVTPDENGNIKGLSGNSLKLSPELMALNTDPEKAVRVGLKSFSDLLPSKVRNNIVAQAKLKHWDKPHKTATANASRKIVEFESQNPGEASKLPWDKKILKENLDFELEMLNSYEKVYGDIKTSYDCILFPTADGWLTIVDTTEQGDLDQALRIGEYSRTHETRNVDDFLSISVNVHDEGNVLEVVGMSSPHGTHVSSIASGNHSSRDVDGVAPNAKIVSMTIGDGRLGSMETGTALVRAMTKVMELCRDGRRIDVINMSYGEHANWSNSGRIGELMNEVVNKYGVVWVASAGNHGPALCTVGTPPDISQPSLIGVGAYVSPQMMEAEYAMREKLPGNVYTWTSRDPCIDGGQGVTVCAPGGAIASVPQFTMSKSQLMNGTSMAAPHVAGAVALLISGLKQQNIEYSPYSIKRAISVTATKLGYVDPFAQGHGLLNVEKAFEHLTEHRQSKDNMLRFSVRVGNNADKGIHLRQGVQRNSIDYNVYIEPIFYNDKEADPKDKFNFNVRLNLIASQPWVQCGAFLDLSYGTRSIAVRVDPTGLQPGVHSAVIRAYDTDCVQKGSLFEIPVTVVQPHVLESDQNTPVFEPASSKGDNSVEFQPNTIQRDFILVPERATWAELRMRITDPNRGEDIGKFFVHTNQLLPKQSCRKLETMKIVSVGSENESIMAFKVKSGRILELCIAKYWSNYGQSHLKYSLRFRGVEAHNPNAYVMHAGRGIHKLEIEALVAEDVQPQLQLKNAEVVLKPTEAKISPLSATRDVIPDGRQVYQNLLAFNLNVAKAADVSIYAPIFNDLLYEAEFESQMWMLFDANKALVATGDAHSHTSFTKLDKGEYTIRLQVRHEKRDLLEKISEANLVASFKLTSPLTLDFYENYNQCIVGGRKYVSSPLRLSTRVLYIAPITQERLTKANLPAQCAWLSGNLVFPQDEVGRRVAQHPFTYILNPAEKKSHTNGSSNGSSAAGSTATAAAVTTANGAKPKAPATPQAATSVTNPAAGDGISVQNDPPVDSSGSPASPKKGKANADDYAESFRDFQCSQIVKCELEMAEKIYNDVVAAHPKHLQANLLLIQNIESNQLKSQLPLTFVNAQKTSPPEAGESADKQKEDQKKVRSALERIVKLADKVIQETDSEALLSYYGLKNDTRADAAKIKTNMDKQKNTLIEALSKKGIAVAKLAVLDDCIKDSLAEINELYTEIIKFVDANDSKAIQFALWHAYAHGHYGRMYKYVVKLIEEKRTRDHFVELAAINGALGHEHIRTVINRMMITAFPSSFRLF

Target ORF information:

RefSeq Version	NM_001299447.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster tripeptidyl-peptidase II (TppII), transcript variant F, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299447.1

ORF Insert Sequence:

ATGGCCACCA GCGGTATTGT CGAGTCATTT CCCACGGGCG CACTGGTGCC CAAGGCGGAG 
ACGGGAGTCC TGAACTTTCT GCAGAAGTAC CCGGAGTACG ATGGACGCGA CGTCACCATA 
GCCATCTTCG ATTCCGGCGT CGATCCCCGG GCAACGGGAC TGGAGACGCT GTGCGATGGA 
AAGACCGTTA AAGTAATAGA GCGGTACGAC TGTTCCGGAT GCGGCGACGT GGACATGAAG 
AAGAAGGTGA CGCCGGACGA GAACGGCAAC ATTAAGGGCC TGTCCGGAAA CTCGCTCAAG 
CTGAGTCCGG AGCTGATGGC TCTGAACACA GATCCGGAGA AGGCGGTGCG GGTAGGCCTC 
AAGAGCTTCA GCGATTTGTT GCCCTCCAAG GTGCGGAACA ACATTGTGGC CCAGGCCAAG 
CTGAAGCACT GGGACAAGCC GCACAAGACG GCCACTGCAA ACGCCAGTCG CAAGATTGTT 
GAATTTGAGT CACAAAATCC AGGAGAAGCC TCAAAACTGC CCTGGGACAA GAAGATATTG 
AAAGAGAATC TAGACTTTGA GCTAGAGATG TTGAATAGCT ACGAGAAGGT GTACGGCGAC 
ATTAAGACCT CCTACGATTG CATCCTTTTT CCCACGGCCG ACGGATGGCT GACAATCGTC 
GACACCACGG AGCAGGGCGA TCTGGATCAG GCTCTGCGCA TTGGCGAGTA TTCCCGCACG 
CACGAGACCC GCAATGTGGA CGACTTTCTT TCCATATCGG TAAACGTCCA CGACGAGGGC 
AACGTACTGG AGGTGGTCGG CATGAGCTCA CCCCACGGCA CCCACGTATC GTCTATTGCC 
AGCGGCAACC ATAGCTCCCG GGACGTGGAT GGCGTGGCGC CGAATGCTAA GATCGTTTCT 
ATGACCATCG GTGATGGTCG ACTTGGGTCT ATGGAGACCG GCACGGCACT AGTGCGTGCC 
ATGACTAAGG TGATGGAACT ATGTCGCGAT GGCAGACGCA TCGATGTGAT CAACATGAGC 
TATGGCGAGC ATGCCAATTG GTCAAATTCC GGCCGCATTG GGGAGCTCAT GAACGAGGTT 
GTCAACAAGT ATGGCGTGGT GTGGGTGGCA TCGGCCGGCA ACCATGGTCC GGCACTTTGT 
ACTGTGGGAA CTCCGCCGGA CATCAGCCAG CCCAGTTTGA TCGGCGTGGG CGCGTACGTA 
TCACCCCAAA TGATGGAGGC CGAGTATGCG ATGCGAGAAA AGCTGCCCGG GAACGTGTAC 
ACCTGGACAT CGCGAGATCC CTGCATCGAC GGAGGTCAAG GCGTTACCGT ATGCGCTCCG 
GGCGGAGCCA TTGCGTCCGT GCCACAGTTT ACTATGAGCA AGTCCCAGCT GATGAACGGT 
ACCAGCATGG CGGCACCTCA CGTCGCCGGC GCAGTGGCGC TGCTCATCTC CGGTCTGAAG 
CAACAGAACA TCGAGTATTC GCCGTACAGT ATTAAGCGAG CGATCAGCGT CACTGCCACC 
AAACTGGGCT ATGTAGATCC CTTTGCTCAG GGCCATGGCT TGCTCAATGT CGAGAAGGCA 
TTCGAGCATT TGACGGAGCA CCGCCAGTCC AAGGATAATA TGCTCAGGTT CTCCGTGCGC 
GTGGGCAACA ACGCAGACAA GGGCATTCAT TTGCGTCAGG GCGTGCAGCG TAACTCCATC 
GATTACAACG TTTATATAGA GCCCATCTTC TACAATGACA AGGAGGCGGA TCCCAAGGAC 
AAGTTTAACT TCAATGTACG GCTAAATCTG ATTGCCTCGC AGCCGTGGGT GCAGTGTGGA 
GCTTTCTTGG ATCTCAGCTA TGGCACCCGC TCCATTGCCG TGCGCGTCGA TCCCACTGGA 
CTCCAGCCAG GCGTTCACAG CGCTGTGATT CGGGCTTATG ACACTGACTG CGTACAGAAG 
GGTTCTCTCT TTGAGATTCC TGTCACGGTG GTGCAGCCCC ATGTGCTGGA GTCGGATCAG 
AACACGCCCG TCTTCGAACC CGCCTCTTCC AAGGGAGACA ACAGCGTGGA GTTTCAGCCA 
AACACCATTC AAAGAGACTT CATCCTGGTG CCAGAACGTG CTACTTGGGC GGAGTTGCGT 
ATGCGTATAA CCGATCCCAA TCGTGGCGAG GACATTGGAA AGTTCTTTGT ACACACGAAC 
CAACTTCTTC CCAAGCAATC CTGTCGTAAG CTTGAGACCA TGAAGATCGT ATCGGTTGGC 
TCGGAAAACG AATCTATAAT GGCTTTTAAA GTTAAGTCTG GCAGGATTCT GGAGCTATGC 
ATTGCCAAGT ACTGGTCCAA CTACGGCCAG AGTCACCTGA AGTACAGCTT GCGTTTCCGT 
GGCGTGGAAG CGCACAATCC CAATGCCTAC GTCATGCATG CGGGCAGGGG AATTCATAAG 
CTGGAGATTG AGGCCCTGGT TGCCGAGGAT GTGCAGCCCC AGCTACAGCT AAAGAATGCC 
GAAGTGGTGC TAAAACCGAC CGAGGCCAAG ATCTCGCCGC TAAGCGCCAC ACGAGACGTC 
ATCCCAGATG GACGTCAGGT GTATCAGAAC CTGCTGGCCT TTAACTTGAA CGTGGCCAAG 
GCCGCAGATG TGTCGATATA CGCACCGATC TTTAACGACT TGTTGTATGA AGCGGAGTTT 
GAGTCACAGA TGTGGATGCT TTTTGATGCA AACAAGGCCC TGGTGGCCAC CGGCGATGCT 
CACTCCCACA CTTCCTTCAC AAAGCTCGAT AAGGGCGAAT ACACAATCAG ACTGCAGGTG 
CGTCACGAGA AGCGCGACCT GCTGGAGAAG ATCTCGGAGG CAAATCTGGT GGCCTCATTC 
AAGCTGACTA GCCCCCTCAC CCTTGATTTC TATGAGAACT ACAATCAGTG CATAGTGGGA 
GGTCGTAAAT ACGTCTCGAG TCCGCTAAGG CTGTCCACTC GGGTGCTTTA TATCGCTCCT 
ATTACCCAAG AGCGACTTAC CAAGGCCAAT CTGCCCGCTC AATGCGCCTG GCTGAGCGGC 
AATCTGGTAT TCCCGCAAGA TGAGGTCGGA CGGCGAGTGG CTCAGCATCC ATTTACTTAC 
ATCCTCAATC CCGCTGAGAA AAAGTCACAC ACGAATGGCT CGAGCAACGG TTCCAGCGCT 
GCAGGATCCA CAGCTACGGC AGCTGCTGTC ACCACTGCTA ATGGCGCAAA ACCGAAAGCT 
CCGGCGACTC CACAAGCGGC CACCTCAGTG ACCAATCCTG CGGCCGGCGA TGGAATTTCT 
GTTCAGAACG ACCCGCCTGT GGACAGCAGT GGGAGTCCCG CCTCACCCAA AAAGGGCAAG 
GCCAATGCCG ACGATTATGC CGAAAGTTTT CGCGACTTTC AATGTTCGCA GATTGTCAAG 
TGTGAACTGG AAATGGCAGA GAAAATCTAT AATGATGTAG TCGCTGCCCA TCCCAAGCAT 
TTGCAGGCAA ACCTGCTACT TATCCAGAAC ATCGAGTCCA ATCAGCTGAA GTCGCAACTG 
CCGCTGACTT TCGTCAATGC TCAAAAGACA TCGCCACCAG AGGCAGGCGA GAGCGCCGAC 
AAGCAAAAGG AGGATCAAAA GAAAGTACGA AGCGCCCTGG AGCGAATTGT TAAGCTGGCC 
GACAAGGTGA TCCAGGAGAC CGATTCCGAG GCACTGCTCT CCTACTACGG TCTGAAGAAC 
GACACTCGTG CCGATGCAGC CAAGATAAAG ACCAACATGG ACAAACAGAA AAACACTCTC 
ATTGAGGCGC TAAGCAAGAA GGGCATTGCT GTGGCAAAGC TGGCTGTATT AGACGACTGT 
ATCAAGGATA GCCTGGCTGA GATCAACGAG CTGTATACCG AGATTATCAA GTTCGTGGAC 
GCCAACGACT CCAAGGCCAT CCAGTTCGCT CTGTGGCACG CCTATGCCCA TGGCCACTAC 
GGTCGCATGT ACAAGTATGT GGTTAAGCTA ATCGAGGAGA AGCGAACCCG CGATCACTTC 
GTGGAGCTGG CCGCCATCAA CGGCGCCCTG GGTCACGAGC ACATCCGTAC TGTCATCAAC 
CGTATGATGA TCACTGCCTT TCCCAGCAGC TTCCGTTTGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TppII ( NM_057899.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_477247.1 Drosophila melanogaster tripeptidyl-peptidase II (TppII), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa12965
Clone ID Related Accession (Same CDS sequence)	NM_001299447.1 , NM_057899.3
Accession Version	NM_057899.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4065bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	tripeptidyl-peptidase II, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). On Jul 15, 2014 this sequence version replaced NM_057899.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCACCA GCGGTATTGT CGAGTCATTT CCCACGGGCG CACTGGTGCC CAAGGCGGAG 
ACGGGAGTCC TGAACTTTCT GCAGAAGTAC CCGGAGTACG ATGGACGCGA CGTCACCATA 
GCCATCTTCG ATTCCGGCGT CGATCCCCGG GCAACGGGAC TGGAGACGCT GTGCGATGGA 
AAGACCGTTA AAGTAATAGA GCGGTACGAC TGTTCCGGAT GCGGCGACGT GGACATGAAG 
AAGAAGGTGA CGCCGGACGA GAACGGCAAC ATTAAGGGCC TGTCCGGAAA CTCGCTCAAG 
CTGAGTCCGG AGCTGATGGC TCTGAACACA GATCCGGAGA AGGCGGTGCG GGTAGGCCTC 
AAGAGCTTCA GCGATTTGTT GCCCTCCAAG GTGCGGAACA ACATTGTGGC CCAGGCCAAG 
CTGAAGCACT GGGACAAGCC GCACAAGACG GCCACTGCAA ACGCCAGTCG CAAGATTGTT 
GAATTTGAGT CACAAAATCC AGGAGAAGCC TCAAAACTGC CCTGGGACAA GAAGATATTG 
AAAGAGAATC TAGACTTTGA GCTAGAGATG TTGAATAGCT ACGAGAAGGT GTACGGCGAC 
ATTAAGACCT CCTACGATTG CATCCTTTTT CCCACGGCCG ACGGATGGCT GACAATCGTC 
GACACCACGG AGCAGGGCGA TCTGGATCAG GCTCTGCGCA TTGGCGAGTA TTCCCGCACG 
CACGAGACCC GCAATGTGGA CGACTTTCTT TCCATATCGG TAAACGTCCA CGACGAGGGC 
AACGTACTGG AGGTGGTCGG CATGAGCTCA CCCCACGGCA CCCACGTATC GTCTATTGCC 
AGCGGCAACC ATAGCTCCCG GGACGTGGAT GGCGTGGCGC CGAATGCTAA GATCGTTTCT 
ATGACCATCG GTGATGGTCG ACTTGGGTCT ATGGAGACCG GCACGGCACT AGTGCGTGCC 
ATGACTAAGG TGATGGAACT ATGTCGCGAT GGCAGACGCA TCGATGTGAT CAACATGAGC 
TATGGCGAGC ATGCCAATTG GTCAAATTCC GGCCGCATTG GGGAGCTCAT GAACGAGGTT 
GTCAACAAGT ATGGCGTGGT GTGGGTGGCA TCGGCCGGCA ACCATGGTCC GGCACTTTGT 
ACTGTGGGAA CTCCGCCGGA CATCAGCCAG CCCAGTTTGA TCGGCGTGGG CGCGTACGTA 
TCACCCCAAA TGATGGAGGC CGAGTATGCG ATGCGAGAAA AGCTGCCCGG GAACGTGTAC 
ACCTGGACAT CGCGAGATCC CTGCATCGAC GGAGGTCAAG GCGTTACCGT ATGCGCTCCG 
GGCGGAGCCA TTGCGTCCGT GCCACAGTTT ACTATGAGCA AGTCCCAGCT GATGAACGGT 
ACCAGCATGG CGGCACCTCA CGTCGCCGGC GCAGTGGCGC TGCTCATCTC CGGTCTGAAG 
CAACAGAACA TCGAGTATTC GCCGTACAGT ATTAAGCGAG CGATCAGCGT CACTGCCACC 
AAACTGGGCT ATGTAGATCC CTTTGCTCAG GGCCATGGCT TGCTCAATGT CGAGAAGGCA 
TTCGAGCATT TGACGGAGCA CCGCCAGTCC AAGGATAATA TGCTCAGGTT CTCCGTGCGC 
GTGGGCAACA ACGCAGACAA GGGCATTCAT TTGCGTCAGG GCGTGCAGCG TAACTCCATC 
GATTACAACG TTTATATAGA GCCCATCTTC TACAATGACA AGGAGGCGGA TCCCAAGGAC 
AAGTTTAACT TCAATGTACG GCTAAATCTG ATTGCCTCGC AGCCGTGGGT GCAGTGTGGA 
GCTTTCTTGG ATCTCAGCTA TGGCACCCGC TCCATTGCCG TGCGCGTCGA TCCCACTGGA 
CTCCAGCCAG GCGTTCACAG CGCTGTGATT CGGGCTTATG ACACTGACTG CGTACAGAAG 
GGTTCTCTCT TTGAGATTCC TGTCACGGTG GTGCAGCCCC ATGTGCTGGA GTCGGATCAG 
AACACGCCCG TCTTCGAACC CGCCTCTTCC AAGGGAGACA ACAGCGTGGA GTTTCAGCCA 
AACACCATTC AAAGAGACTT CATCCTGGTG CCAGAACGTG CTACTTGGGC GGAGTTGCGT 
ATGCGTATAA CCGATCCCAA TCGTGGCGAG GACATTGGAA AGTTCTTTGT ACACACGAAC 
CAACTTCTTC CCAAGCAATC CTGTCGTAAG CTTGAGACCA TGAAGATCGT ATCGGTTGGC 
TCGGAAAACG AATCTATAAT GGCTTTTAAA GTTAAGTCTG GCAGGATTCT GGAGCTATGC 
ATTGCCAAGT ACTGGTCCAA CTACGGCCAG AGTCACCTGA AGTACAGCTT GCGTTTCCGT 
GGCGTGGAAG CGCACAATCC CAATGCCTAC GTCATGCATG CGGGCAGGGG AATTCATAAG 
CTGGAGATTG AGGCCCTGGT TGCCGAGGAT GTGCAGCCCC AGCTACAGCT AAAGAATGCC 
GAAGTGGTGC TAAAACCGAC CGAGGCCAAG ATCTCGCCGC TAAGCGCCAC ACGAGACGTC 
ATCCCAGATG GACGTCAGGT GTATCAGAAC CTGCTGGCCT TTAACTTGAA CGTGGCCAAG 
GCCGCAGATG TGTCGATATA CGCACCGATC TTTAACGACT TGTTGTATGA AGCGGAGTTT 
GAGTCACAGA TGTGGATGCT TTTTGATGCA AACAAGGCCC TGGTGGCCAC CGGCGATGCT 
CACTCCCACA CTTCCTTCAC AAAGCTCGAT AAGGGCGAAT ACACAATCAG ACTGCAGGTG 
CGTCACGAGA AGCGCGACCT GCTGGAGAAG ATCTCGGAGG CAAATCTGGT GGCCTCATTC 
AAGCTGACTA GCCCCCTCAC CCTTGATTTC TATGAGAACT ACAATCAGTG CATAGTGGGA 
GGTCGTAAAT ACGTCTCGAG TCCGCTAAGG CTGTCCACTC GGGTGCTTTA TATCGCTCCT 
ATTACCCAAG AGCGACTTAC CAAGGCCAAT CTGCCCGCTC AATGCGCCTG GCTGAGCGGC 
AATCTGGTAT TCCCGCAAGA TGAGGTCGGA CGGCGAGTGG CTCAGCATCC ATTTACTTAC 
ATCCTCAATC CCGCTGAGAA AAAGTCACAC ACGAATGGCT CGAGCAACGG TTCCAGCGCT 
GCAGGATCCA CAGCTACGGC AGCTGCTGTC ACCACTGCTA ATGGCGCAAA ACCGAAAGCT 
CCGGCGACTC CACAAGCGGC CACCTCAGTG ACCAATCCTG CGGCCGGCGA TGGAATTTCT 
GTTCAGAACG ACCCGCCTGT GGACAGCAGT GGGAGTCCCG CCTCACCCAA AAAGGGCAAG 
GCCAATGCCG ACGATTATGC CGAAAGTTTT CGCGACTTTC AATGTTCGCA GATTGTCAAG 
TGTGAACTGG AAATGGCAGA GAAAATCTAT AATGATGTAG TCGCTGCCCA TCCCAAGCAT 
TTGCAGGCAA ACCTGCTACT TATCCAGAAC ATCGAGTCCA ATCAGCTGAA GTCGCAACTG 
CCGCTGACTT TCGTCAATGC TCAAAAGACA TCGCCACCAG AGGCAGGCGA GAGCGCCGAC 
AAGCAAAAGG AGGATCAAAA GAAAGTACGA AGCGCCCTGG AGCGAATTGT TAAGCTGGCC 
GACAAGGTGA TCCAGGAGAC CGATTCCGAG GCACTGCTCT CCTACTACGG TCTGAAGAAC 
GACACTCGTG CCGATGCAGC CAAGATAAAG ACCAACATGG ACAAACAGAA AAACACTCTC 
ATTGAGGCGC TAAGCAAGAA GGGCATTGCT GTGGCAAAGC TGGCTGTATT AGACGACTGT 
ATCAAGGATA GCCTGGCTGA GATCAACGAG CTGTATACCG AGATTATCAA GTTCGTGGAC 
GCCAACGACT CCAAGGCCAT CCAGTTCGCT CTGTGGCACG CCTATGCCCA TGGCCACTAC 
GGTCGCATGT ACAAGTATGT GGTTAAGCTA ATCGAGGAGA AGCGAACCCG CGATCACTTC 
GTGGAGCTGG CCGCCATCAA CGGCGCCCTG GGTCACGAGC ACATCCGTAC TGTCATCAAC 
CGTATGATGA TCACTGCCTT TCCCAGCAGC TTCCGTTTGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_477247.1
CDS	114..4178
Translation	MATSGIVESFPTGALVPKAETGVLNFLQKYPEYDGRDVTIAIFDSGVDPRATGLETLCDGKTVKVIERYDCSGCGDVDMKKKVTPDENGNIKGLSGNSLKLSPELMALNTDPEKAVRVGLKSFSDLLPSKVRNNIVAQAKLKHWDKPHKTATANASRKIVEFESQNPGEASKLPWDKKILKENLDFELEMLNSYEKVYGDIKTSYDCILFPTADGWLTIVDTTEQGDLDQALRIGEYSRTHETRNVDDFLSISVNVHDEGNVLEVVGMSSPHGTHVSSIASGNHSSRDVDGVAPNAKIVSMTIGDGRLGSMETGTALVRAMTKVMELCRDGRRIDVINMSYGEHANWSNSGRIGELMNEVVNKYGVVWVASAGNHGPALCTVGTPPDISQPSLIGVGAYVSPQMMEAEYAMREKLPGNVYTWTSRDPCIDGGQGVTVCAPGGAIASVPQFTMSKSQLMNGTSMAAPHVAGAVALLISGLKQQNIEYSPYSIKRAISVTATKLGYVDPFAQGHGLLNVEKAFEHLTEHRQSKDNMLRFSVRVGNNADKGIHLRQGVQRNSIDYNVYIEPIFYNDKEADPKDKFNFNVRLNLIASQPWVQCGAFLDLSYGTRSIAVRVDPTGLQPGVHSAVIRAYDTDCVQKGSLFEIPVTVVQPHVLESDQNTPVFEPASSKGDNSVEFQPNTIQRDFILVPERATWAELRMRITDPNRGEDIGKFFVHTNQLLPKQSCRKLETMKIVSVGSENESIMAFKVKSGRILELCIAKYWSNYGQSHLKYSLRFRGVEAHNPNAYVMHAGRGIHKLEIEALVAEDVQPQLQLKNAEVVLKPTEAKISPLSATRDVIPDGRQVYQNLLAFNLNVAKAADVSIYAPIFNDLLYEAEFESQMWMLFDANKALVATGDAHSHTSFTKLDKGEYTIRLQVRHEKRDLLEKISEANLVASFKLTSPLTLDFYENYNQCIVGGRKYVSSPLRLSTRVLYIAPITQERLTKANLPAQCAWLSGNLVFPQDEVGRRVAQHPFTYILNPAEKKSHTNGSSNGSSAAGSTATAAAVTTANGAKPKAPATPQAATSVTNPAAGDGISVQNDPPVDSSGSPASPKKGKANADDYAESFRDFQCSQIVKCELEMAEKIYNDVVAAHPKHLQANLLLIQNIESNQLKSQLPLTFVNAQKTSPPEAGESADKQKEDQKKVRSALERIVKLADKVIQETDSEALLSYYGLKNDTRADAAKIKTNMDKQKNTLIEALSKKGIAVAKLAVLDDCIKDSLAEINELYTEIIKFVDANDSKAIQFALWHAYAHGHYGRMYKYVVKLIEEKRTRDHFVELAAINGALGHEHIRTVINRMMITAFPSSFRLF

Target ORF information:

RefSeq Version	NM_057899.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster tripeptidyl-peptidase II (TppII), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057899.3

ORF Insert Sequence:

ATGGCCACCA GCGGTATTGT CGAGTCATTT CCCACGGGCG CACTGGTGCC CAAGGCGGAG 
ACGGGAGTCC TGAACTTTCT GCAGAAGTAC CCGGAGTACG ATGGACGCGA CGTCACCATA 
GCCATCTTCG ATTCCGGCGT CGATCCCCGG GCAACGGGAC TGGAGACGCT GTGCGATGGA 
AAGACCGTTA AAGTAATAGA GCGGTACGAC TGTTCCGGAT GCGGCGACGT GGACATGAAG 
AAGAAGGTGA CGCCGGACGA GAACGGCAAC ATTAAGGGCC TGTCCGGAAA CTCGCTCAAG 
CTGAGTCCGG AGCTGATGGC TCTGAACACA GATCCGGAGA AGGCGGTGCG GGTAGGCCTC 
AAGAGCTTCA GCGATTTGTT GCCCTCCAAG GTGCGGAACA ACATTGTGGC CCAGGCCAAG 
CTGAAGCACT GGGACAAGCC GCACAAGACG GCCACTGCAA ACGCCAGTCG CAAGATTGTT 
GAATTTGAGT CACAAAATCC AGGAGAAGCC TCAAAACTGC CCTGGGACAA GAAGATATTG 
AAAGAGAATC TAGACTTTGA GCTAGAGATG TTGAATAGCT ACGAGAAGGT GTACGGCGAC 
ATTAAGACCT CCTACGATTG CATCCTTTTT CCCACGGCCG ACGGATGGCT GACAATCGTC 
GACACCACGG AGCAGGGCGA TCTGGATCAG GCTCTGCGCA TTGGCGAGTA TTCCCGCACG 
CACGAGACCC GCAATGTGGA CGACTTTCTT TCCATATCGG TAAACGTCCA CGACGAGGGC 
AACGTACTGG AGGTGGTCGG CATGAGCTCA CCCCACGGCA CCCACGTATC GTCTATTGCC 
AGCGGCAACC ATAGCTCCCG GGACGTGGAT GGCGTGGCGC CGAATGCTAA GATCGTTTCT 
ATGACCATCG GTGATGGTCG ACTTGGGTCT ATGGAGACCG GCACGGCACT AGTGCGTGCC 
ATGACTAAGG TGATGGAACT ATGTCGCGAT GGCAGACGCA TCGATGTGAT CAACATGAGC 
TATGGCGAGC ATGCCAATTG GTCAAATTCC GGCCGCATTG GGGAGCTCAT GAACGAGGTT 
GTCAACAAGT ATGGCGTGGT GTGGGTGGCA TCGGCCGGCA ACCATGGTCC GGCACTTTGT 
ACTGTGGGAA CTCCGCCGGA CATCAGCCAG CCCAGTTTGA TCGGCGTGGG CGCGTACGTA 
TCACCCCAAA TGATGGAGGC CGAGTATGCG ATGCGAGAAA AGCTGCCCGG GAACGTGTAC 
ACCTGGACAT CGCGAGATCC CTGCATCGAC GGAGGTCAAG GCGTTACCGT ATGCGCTCCG 
GGCGGAGCCA TTGCGTCCGT GCCACAGTTT ACTATGAGCA AGTCCCAGCT GATGAACGGT 
ACCAGCATGG CGGCACCTCA CGTCGCCGGC GCAGTGGCGC TGCTCATCTC CGGTCTGAAG 
CAACAGAACA TCGAGTATTC GCCGTACAGT ATTAAGCGAG CGATCAGCGT CACTGCCACC 
AAACTGGGCT ATGTAGATCC CTTTGCTCAG GGCCATGGCT TGCTCAATGT CGAGAAGGCA 
TTCGAGCATT TGACGGAGCA CCGCCAGTCC AAGGATAATA TGCTCAGGTT CTCCGTGCGC 
GTGGGCAACA ACGCAGACAA GGGCATTCAT TTGCGTCAGG GCGTGCAGCG TAACTCCATC 
GATTACAACG TTTATATAGA GCCCATCTTC TACAATGACA AGGAGGCGGA TCCCAAGGAC 
AAGTTTAACT TCAATGTACG GCTAAATCTG ATTGCCTCGC AGCCGTGGGT GCAGTGTGGA 
GCTTTCTTGG ATCTCAGCTA TGGCACCCGC TCCATTGCCG TGCGCGTCGA TCCCACTGGA 
CTCCAGCCAG GCGTTCACAG CGCTGTGATT CGGGCTTATG ACACTGACTG CGTACAGAAG 
GGTTCTCTCT TTGAGATTCC TGTCACGGTG GTGCAGCCCC ATGTGCTGGA GTCGGATCAG 
AACACGCCCG TCTTCGAACC CGCCTCTTCC AAGGGAGACA ACAGCGTGGA GTTTCAGCCA 
AACACCATTC AAAGAGACTT CATCCTGGTG CCAGAACGTG CTACTTGGGC GGAGTTGCGT 
ATGCGTATAA CCGATCCCAA TCGTGGCGAG GACATTGGAA AGTTCTTTGT ACACACGAAC 
CAACTTCTTC CCAAGCAATC CTGTCGTAAG CTTGAGACCA TGAAGATCGT ATCGGTTGGC 
TCGGAAAACG AATCTATAAT GGCTTTTAAA GTTAAGTCTG GCAGGATTCT GGAGCTATGC 
ATTGCCAAGT ACTGGTCCAA CTACGGCCAG AGTCACCTGA AGTACAGCTT GCGTTTCCGT 
GGCGTGGAAG CGCACAATCC CAATGCCTAC GTCATGCATG CGGGCAGGGG AATTCATAAG 
CTGGAGATTG AGGCCCTGGT TGCCGAGGAT GTGCAGCCCC AGCTACAGCT AAAGAATGCC 
GAAGTGGTGC TAAAACCGAC CGAGGCCAAG ATCTCGCCGC TAAGCGCCAC ACGAGACGTC 
ATCCCAGATG GACGTCAGGT GTATCAGAAC CTGCTGGCCT TTAACTTGAA CGTGGCCAAG 
GCCGCAGATG TGTCGATATA CGCACCGATC TTTAACGACT TGTTGTATGA AGCGGAGTTT 
GAGTCACAGA TGTGGATGCT TTTTGATGCA AACAAGGCCC TGGTGGCCAC CGGCGATGCT 
CACTCCCACA CTTCCTTCAC AAAGCTCGAT AAGGGCGAAT ACACAATCAG ACTGCAGGTG 
CGTCACGAGA AGCGCGACCT GCTGGAGAAG ATCTCGGAGG CAAATCTGGT GGCCTCATTC 
AAGCTGACTA GCCCCCTCAC CCTTGATTTC TATGAGAACT ACAATCAGTG CATAGTGGGA 
GGTCGTAAAT ACGTCTCGAG TCCGCTAAGG CTGTCCACTC GGGTGCTTTA TATCGCTCCT 
ATTACCCAAG AGCGACTTAC CAAGGCCAAT CTGCCCGCTC AATGCGCCTG GCTGAGCGGC 
AATCTGGTAT TCCCGCAAGA TGAGGTCGGA CGGCGAGTGG CTCAGCATCC ATTTACTTAC 
ATCCTCAATC CCGCTGAGAA AAAGTCACAC ACGAATGGCT CGAGCAACGG TTCCAGCGCT 
GCAGGATCCA CAGCTACGGC AGCTGCTGTC ACCACTGCTA ATGGCGCAAA ACCGAAAGCT 
CCGGCGACTC CACAAGCGGC CACCTCAGTG ACCAATCCTG CGGCCGGCGA TGGAATTTCT 
GTTCAGAACG ACCCGCCTGT GGACAGCAGT GGGAGTCCCG CCTCACCCAA AAAGGGCAAG 
GCCAATGCCG ACGATTATGC CGAAAGTTTT CGCGACTTTC AATGTTCGCA GATTGTCAAG 
TGTGAACTGG AAATGGCAGA GAAAATCTAT AATGATGTAG TCGCTGCCCA TCCCAAGCAT 
TTGCAGGCAA ACCTGCTACT TATCCAGAAC ATCGAGTCCA ATCAGCTGAA GTCGCAACTG 
CCGCTGACTT TCGTCAATGC TCAAAAGACA TCGCCACCAG AGGCAGGCGA GAGCGCCGAC 
AAGCAAAAGG AGGATCAAAA GAAAGTACGA AGCGCCCTGG AGCGAATTGT TAAGCTGGCC 
GACAAGGTGA TCCAGGAGAC CGATTCCGAG GCACTGCTCT CCTACTACGG TCTGAAGAAC 
GACACTCGTG CCGATGCAGC CAAGATAAAG ACCAACATGG ACAAACAGAA AAACACTCTC 
ATTGAGGCGC TAAGCAAGAA GGGCATTGCT GTGGCAAAGC TGGCTGTATT AGACGACTGT 
ATCAAGGATA GCCTGGCTGA GATCAACGAG CTGTATACCG AGATTATCAA GTTCGTGGAC 
GCCAACGACT CCAAGGCCAT CCAGTTCGCT CTGTGGCACG CCTATGCCCA TGGCCACTAC 
GGTCGCATGT ACAAGTATGT GGTTAAGCTA ATCGAGGAGA AGCGAACCCG CGATCACTTC 
GTGGAGCTGG CCGCCATCAA CGGCGCCCTG GGTCACGAGC ACATCCGTAC TGTCATCAAC 
CGTATGATGA TCACTGCCTT TCCCAGCAGC TTCCGTTTGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TppII ( NM_001299448.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001286377.1 Drosophila melanogaster tripeptidyl-peptidase II (TppII), transcript variant E, mRNA.

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CloneID	OFa21796
Clone ID Related Accession (Same CDS sequence)	NM_001299448.1
Accession Version	NM_001299448.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4128bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	tripeptidyl-peptidase II, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033778). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCACCA GCGGTATTGT CGAGTCATTT CCCACGGGCG CACTGGTGCC CAAGGCGGAG 
ACGGGAGTCC TGAACTTTCT GCAGAAGTAC CCGGAGTACG ATGGACGCGA CGTCACCATA 
GCCATCTTCG ATTCCGGCGT CGATCCCCGG GCAACGGGAC TGGAGACGCT GTGCGATGGA 
AAGACCGTTA AAGTAATAGA GCGGTACGAC TGTTCCGGAT GCGGCGACGT GGACATGAAG 
AAGAAGGTGA CGCCGGACGA GAACGGCAAC ATTAAGGGCC TGTCCGGAAA CTCGCTCAAG 
CTGAGTCCGG AGCTGATGGC TCTGAACACA GATCCGGAGA AGGCGGTGCG GGTAGGCCTC 
AAGAGCTTCA GCGATTTGTT GCCCTCCAAG GTGCGGAACA ACATTGTGGC CCAGGCCAAG 
CTGAAGCACT GGGACAAGCC GCACAAGACG GCCACTGCAA ACGCCAGTCG CAAGATTGTT 
GAATTTGAGT CACAAAATCC AGGAGAAGCC TCAAAACTGC CCTGGGACAA GAAGATATTG 
AAAGAGAATC TAGACTTTGA GCTAGAGATG TTGAATAGCT ACGAGAAGGT GTACGGCGAC 
ATTAAGACCT CCTACGATTG CATCCTTTTT CCCACGGCCG ACGGATGGCT GACAATCGTC 
GACACCACGG AGCAGGGCGA TCTGGATCAG GCTCTGCGCA TTGGCGAGTA TTCCCGCACG 
CACGAGACCC GCAATGTGGA CGACTTTCTT TCCATATCGG TAAACGTCCA CGACGAGGGC 
AACGTACTGG AGGTGGTCGG CATGAGCTCA CCCCACGGCA CCCACGTATC GTCTATTGCC 
AGCGGCAACC ATAGCTCCCG GGACGTGGAT GGCGTGGCGC CGAATGCTAA GATCGTTTCT 
ATGACCATCG GTGATGGTCG ACTTGGGTCT ATGGAGACCG GCACGGCACT AGTGCGTGCC 
ATGACTAAGG TGATGGAACT ATGTCGCGAT GGCAGACGCA TCGATGTGAT CAACATGAGC 
TATGGCGAGC ATGCCAATTG GTCAAATTCC GGTGTGTCTG GGTCAGGGTC TGGGTCGGAT 
GAAATGCGTC GCAGAAGAAA GATGTGTGCG TCTAGCCGCA TTGGGGAGCT CATGAACGAG 
GTTGTCAACA AGTATGGCGT GGTGTGGGTG GCATCGGCCG GCAACCATGG TCCGGCACTT 
TGTACTGTGG GAACTCCGCC GGACATCAGC CAGCCCAGTT TGATCGGCGT GGGCGCGTAC 
GTATCACCCC AAATGATGGA GGCCGAGTAT GCGATGCGAG AAAAGCTGCC CGGGAACGTG 
TACACCTGGA CATCGCGAGA TCCCTGCATC GACGGAGGTC AAGGCGTTAC CGTATGCGCT 
CCGGGCGGAG CCATTGCGTC CGTGCCACAG TTTACTATGA GCAAGTCCCA GCTGATGAAC 
GGTACCAGCA TGGCGGCACC TCACGTCGCC GGCGCAGTGG CGCTGCTCAT CTCCGGTCTG 
AAGCAACAGA ACATCGAGTA TTCGCCGTAC AGTATTAAGC GAGCGATCAG CGTCACTGCC 
ACCAAACTGG GCTATGTAGA TCCCTTTGCT CAGGGCCATG GCTTGCTCAA TGTCGAGAAG 
GCATTCGAGC ATTTGACGGA GCACCGCCAG TCCAAGGATA ATATGCTCAG GTTCTCCGTG 
CGCGTGGGCA ACAACGCAGA CAAGGGCATT CATTTGCGTC AGGGCGTGCA GCGTAACTCC 
ATCGATTACA ACGTTTATAT AGAGCCCATC TTCTACAATG ACAAGGAGGC GGATCCCAAG 
GACAAGTTTA ACTTCAATGT ACGGCTAAAT CTGATTGCCT CGCAGCCGTG GGTGCAGTGT 
GGAGCTTTCT TGGATCTCAG CTATGGCACC CGCTCCATTG CCGTGCGCGT CGATCCCACT 
GGACTCCAGC CAGGCGTTCA CAGCGCTGTG ATTCGGGCTT ATGACACTGA CTGCGTACAG 
AAGGGTTCTC TCTTTGAGAT TCCTGTCACG GTGGTGCAGC CCCATGTGCT GGAGTCGGAT 
CAGAACACGC CCGTCTTCGA ACCCGCCTCT TCCAAGGGAG ACAACAGCGT GGAGTTTCAG 
CCAAACACCA TTCAAAGAGA CTTCATCCTG GTGCCAGAAC GTGCTACTTG GGCGGAGTTG 
CGTATGCGTA TAACCGATCC CAATCGTGGC GAGGACATTG GAAAGTTCTT TGTACACACG 
AACCAACTTC TTCCCAAGCA ATCCTGTCGT AAGCTTGAGA CCATGAAGAT CGTATCGGTT 
GGCTCGGAAA ACGAATCTAT AATGGCTTTT AAAGTTAAGT CTGGCAGGAT TCTGGAGCTA 
TGCATTGCCA AGTACTGGTC CAACTACGGC CAGAGTCACC TGAAGTACAG CTTGCGTTTC 
CGTGGCGTGG AAGCGCACAA TCCCAATGCC TACGTCATGC ATGCGGGCAG GGGAATTCAT 
AAGCTGGAGA TTGAGGCCCT GGTTGCCGAG GATGTGCAGC CCCAGCTACA GCTAAAGAAT 
GCCGAAGTGG TGCTAAAACC GACCGAGGCC AAGATCTCGC CGCTAAGCGC CACACGAGAC 
GTCATCCCAG ATGGACGTCA GGTGTATCAG AACCTGCTGG CCTTTAACTT GAACGTGGCC 
AAGGCCGCAG ATGTGTCGAT ATACGCACCG ATCTTTAACG ACTTGTTGTA TGAAGCGGAG 
TTTGAGTCAC AGATGTGGAT GCTTTTTGAT GCAAACAAGG CCCTGGTGGC CACCGGCGAT 
GCTCACTCCC ACACTTCCTT CACAAAGCTC GATAAGGGCG AATACACAAT CAGACTGCAG 
GTGCGTCACG AGAAGCGCGA CCTGCTGGAG AAGATCTCGG AGGCAAATCT GGTGGCCTCA 
TTCAAGCTGA CTAGCCCCCT CACCCTTGAT TTCTATGAGA ACTACAATCA GTGCATAGTG 
GGAGGTCGTA AATACGTCTC GAGTCCGCTA AGGCTGTCCA CTCGGGTGCT TTATATCGCT 
CCTATTACCC AAGAGCGACT TACCAAGGCC AATCTGCCCG CTCAATGCGC CTGGCTGAGC 
GGCAATCTGG TATTCCCGCA AGATGAGGTC GGACGGCGAG TGGCTCAGCA TCCATTTACT 
TACATCCTCA ATCCCGCTGA GAAAAAGTCA CACACGAATG GCTCGAGCAA CGGTTCCAGC 
GCTGCAGGAT CCACAGCTAC GGCAGCTGCT GTCACCACTG CTAATGGCGC AAAACCGAAA 
GCTCCGGCGA CTCCACAAGC GGCCACCTCA GTGACCAATC CTGCGGCCGG CGATGGAATT 
TCTGTTCAGA ACGACCCGCC TGTGGACAGC AGTGGGAGTC CCGCCTCACC CAAAAAGGGC 
AAGGCCAATG CCGACGATTA TGCCGAAAGT TTTCGCGACT TTCAATGTTC GCAGATTGTC 
AAGTGTGAAC TGGAAATGGC AGAGAAAATC TATAATGATG TAGTCGCTGC CCATCCCAAG 
CATTTGCAGG CAAACCTGCT ACTTATCCAG AACATCGAGT CCAATCAGCT GAAGTCGCAA 
CTGCCGCTGA CTTTCGTCAA TGCTCAAAAG ACATCGCCAC CAGAGGCAGG CGAGAGCGCC 
GACAAGCAAA AGGAGGATCA AAAGAAAGTA CGAAGCGCCC TGGAGCGAAT TGTTAAGCTG 
GCCGACAAGG TGATCCAGGA GACCGATTCC GAGGCACTGC TCTCCTACTA CGGTCTGAAG 
AACGACACTC GTGCCGATGC AGCCAAGATA AAGACCAACA TGGACAAACA GAAAAACACT 
CTCATTGAGG CGCTAAGCAA GAAGGGCATT GCTGTGGCAA AGCTGGCTGT ATTAGACGAC 
TGTATCAAGG ATAGCCTGGC TGAGATCAAC GAGCTGTATA CCGAGATTAT CAAGTTCGTG 
GACGCCAACG ACTCCAAGGC CATCCAGTTC GCTCTGTGGC ACGCCTATGC CCATGGCCAC 
TACGGTCGCA TGTACAAGTA TGTGGTTAAG CTAATCGAGG AGAAGCGAAC CCGCGATCAC 
TTCGTGGAGC TGGCCGCCAT CAACGGCGCC CTGGGTCACG AGCACATCCG TACTGTCATC 
AACCGTATGA TGATCACTGC CTTTCCCAGC AGCTTCCGTT TGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001286377.1
CDS	114..4241
Translation	MATSGIVESFPTGALVPKAETGVLNFLQKYPEYDGRDVTIAIFDSGVDPRATGLETLCDGKTVKVIERYDCSGCGDVDMKKKVTPDENGNIKGLSGNSLKLSPELMALNTDPEKAVRVGLKSFSDLLPSKVRNNIVAQAKLKHWDKPHKTATANASRKIVEFESQNPGEASKLPWDKKILKENLDFELEMLNSYEKVYGDIKTSYDCILFPTADGWLTIVDTTEQGDLDQALRIGEYSRTHETRNVDDFLSISVNVHDEGNVLEVVGMSSPHGTHVSSIASGNHSSRDVDGVAPNAKIVSMTIGDGRLGSMETGTALVRAMTKVMELCRDGRRIDVINMSYGEHANWSNSGVSGSGSGSDEMRRRRKMCASSRIGELMNEVVNKYGVVWVASAGNHGPALCTVGTPPDISQPSLIGVGAYVSPQMMEAEYAMREKLPGNVYTWTSRDPCIDGGQGVTVCAPGGAIASVPQFTMSKSQLMNGTSMAAPHVAGAVALLISGLKQQNIEYSPYSIKRAISVTATKLGYVDPFAQGHGLLNVEKAFEHLTEHRQSKDNMLRFSVRVGNNADKGIHLRQGVQRNSIDYNVYIEPIFYNDKEADPKDKFNFNVRLNLIASQPWVQCGAFLDLSYGTRSIAVRVDPTGLQPGVHSAVIRAYDTDCVQKGSLFEIPVTVVQPHVLESDQNTPVFEPASSKGDNSVEFQPNTIQRDFILVPERATWAELRMRITDPNRGEDIGKFFVHTNQLLPKQSCRKLETMKIVSVGSENESIMAFKVKSGRILELCIAKYWSNYGQSHLKYSLRFRGVEAHNPNAYVMHAGRGIHKLEIEALVAEDVQPQLQLKNAEVVLKPTEAKISPLSATRDVIPDGRQVYQNLLAFNLNVAKAADVSIYAPIFNDLLYEAEFESQMWMLFDANKALVATGDAHSHTSFTKLDKGEYTIRLQVRHEKRDLLEKISEANLVASFKLTSPLTLDFYENYNQCIVGGRKYVSSPLRLSTRVLYIAPITQERLTKANLPAQCAWLSGNLVFPQDEVGRRVAQHPFTYILNPAEKKSHTNGSSNGSSAAGSTATAAAVTTANGAKPKAPATPQAATSVTNPAAGDGISVQNDPPVDSSGSPASPKKGKANADDYAESFRDFQCSQIVKCELEMAEKIYNDVVAAHPKHLQANLLLIQNIESNQLKSQLPLTFVNAQKTSPPEAGESADKQKEDQKKVRSALERIVKLADKVIQETDSEALLSYYGLKNDTRADAAKIKTNMDKQKNTLIEALSKKGIAVAKLAVLDDCIKDSLAEINELYTEIIKFVDANDSKAIQFALWHAYAHGHYGRMYKYVVKLIEEKRTRDHFVELAAINGALGHEHIRTVINRMMITAFPSSFRLF

Target ORF information:

RefSeq Version	NM_001299448.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster tripeptidyl-peptidase II (TppII), transcript variant E, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001299448.1

ORF Insert Sequence:

ATGGCCACCA GCGGTATTGT CGAGTCATTT CCCACGGGCG CACTGGTGCC CAAGGCGGAG 
ACGGGAGTCC TGAACTTTCT GCAGAAGTAC CCGGAGTACG ATGGACGCGA CGTCACCATA 
GCCATCTTCG ATTCCGGCGT CGATCCCCGG GCAACGGGAC TGGAGACGCT GTGCGATGGA 
AAGACCGTTA AAGTAATAGA GCGGTACGAC TGTTCCGGAT GCGGCGACGT GGACATGAAG 
AAGAAGGTGA CGCCGGACGA GAACGGCAAC ATTAAGGGCC TGTCCGGAAA CTCGCTCAAG 
CTGAGTCCGG AGCTGATGGC TCTGAACACA GATCCGGAGA AGGCGGTGCG GGTAGGCCTC 
AAGAGCTTCA GCGATTTGTT GCCCTCCAAG GTGCGGAACA ACATTGTGGC CCAGGCCAAG 
CTGAAGCACT GGGACAAGCC GCACAAGACG GCCACTGCAA ACGCCAGTCG CAAGATTGTT 
GAATTTGAGT CACAAAATCC AGGAGAAGCC TCAAAACTGC CCTGGGACAA GAAGATATTG 
AAAGAGAATC TAGACTTTGA GCTAGAGATG TTGAATAGCT ACGAGAAGGT GTACGGCGAC 
ATTAAGACCT CCTACGATTG CATCCTTTTT CCCACGGCCG ACGGATGGCT GACAATCGTC 
GACACCACGG AGCAGGGCGA TCTGGATCAG GCTCTGCGCA TTGGCGAGTA TTCCCGCACG 
CACGAGACCC GCAATGTGGA CGACTTTCTT TCCATATCGG TAAACGTCCA CGACGAGGGC 
AACGTACTGG AGGTGGTCGG CATGAGCTCA CCCCACGGCA CCCACGTATC GTCTATTGCC 
AGCGGCAACC ATAGCTCCCG GGACGTGGAT GGCGTGGCGC CGAATGCTAA GATCGTTTCT 
ATGACCATCG GTGATGGTCG ACTTGGGTCT ATGGAGACCG GCACGGCACT AGTGCGTGCC 
ATGACTAAGG TGATGGAACT ATGTCGCGAT GGCAGACGCA TCGATGTGAT CAACATGAGC 
TATGGCGAGC ATGCCAATTG GTCAAATTCC GGTGTGTCTG GGTCAGGGTC TGGGTCGGAT 
GAAATGCGTC GCAGAAGAAA GATGTGTGCG TCTAGCCGCA TTGGGGAGCT CATGAACGAG 
GTTGTCAACA AGTATGGCGT GGTGTGGGTG GCATCGGCCG GCAACCATGG TCCGGCACTT 
TGTACTGTGG GAACTCCGCC GGACATCAGC CAGCCCAGTT TGATCGGCGT GGGCGCGTAC 
GTATCACCCC AAATGATGGA GGCCGAGTAT GCGATGCGAG AAAAGCTGCC CGGGAACGTG 
TACACCTGGA CATCGCGAGA TCCCTGCATC GACGGAGGTC AAGGCGTTAC CGTATGCGCT 
CCGGGCGGAG CCATTGCGTC CGTGCCACAG TTTACTATGA GCAAGTCCCA GCTGATGAAC 
GGTACCAGCA TGGCGGCACC TCACGTCGCC GGCGCAGTGG CGCTGCTCAT CTCCGGTCTG 
AAGCAACAGA ACATCGAGTA TTCGCCGTAC AGTATTAAGC GAGCGATCAG CGTCACTGCC 
ACCAAACTGG GCTATGTAGA TCCCTTTGCT CAGGGCCATG GCTTGCTCAA TGTCGAGAAG 
GCATTCGAGC ATTTGACGGA GCACCGCCAG TCCAAGGATA ATATGCTCAG GTTCTCCGTG 
CGCGTGGGCA ACAACGCAGA CAAGGGCATT CATTTGCGTC AGGGCGTGCA GCGTAACTCC 
ATCGATTACA ACGTTTATAT AGAGCCCATC TTCTACAATG ACAAGGAGGC GGATCCCAAG 
GACAAGTTTA ACTTCAATGT ACGGCTAAAT CTGATTGCCT CGCAGCCGTG GGTGCAGTGT 
GGAGCTTTCT TGGATCTCAG CTATGGCACC CGCTCCATTG CCGTGCGCGT CGATCCCACT 
GGACTCCAGC CAGGCGTTCA CAGCGCTGTG ATTCGGGCTT ATGACACTGA CTGCGTACAG 
AAGGGTTCTC TCTTTGAGAT TCCTGTCACG GTGGTGCAGC CCCATGTGCT GGAGTCGGAT 
CAGAACACGC CCGTCTTCGA ACCCGCCTCT TCCAAGGGAG ACAACAGCGT GGAGTTTCAG 
CCAAACACCA TTCAAAGAGA CTTCATCCTG GTGCCAGAAC GTGCTACTTG GGCGGAGTTG 
CGTATGCGTA TAACCGATCC CAATCGTGGC GAGGACATTG GAAAGTTCTT TGTACACACG 
AACCAACTTC TTCCCAAGCA ATCCTGTCGT AAGCTTGAGA CCATGAAGAT CGTATCGGTT 
GGCTCGGAAA ACGAATCTAT AATGGCTTTT AAAGTTAAGT CTGGCAGGAT TCTGGAGCTA 
TGCATTGCCA AGTACTGGTC CAACTACGGC CAGAGTCACC TGAAGTACAG CTTGCGTTTC 
CGTGGCGTGG AAGCGCACAA TCCCAATGCC TACGTCATGC ATGCGGGCAG GGGAATTCAT 
AAGCTGGAGA TTGAGGCCCT GGTTGCCGAG GATGTGCAGC CCCAGCTACA GCTAAAGAAT 
GCCGAAGTGG TGCTAAAACC GACCGAGGCC AAGATCTCGC CGCTAAGCGC CACACGAGAC 
GTCATCCCAG ATGGACGTCA GGTGTATCAG AACCTGCTGG CCTTTAACTT GAACGTGGCC 
AAGGCCGCAG ATGTGTCGAT ATACGCACCG ATCTTTAACG ACTTGTTGTA TGAAGCGGAG 
TTTGAGTCAC AGATGTGGAT GCTTTTTGAT GCAAACAAGG CCCTGGTGGC CACCGGCGAT 
GCTCACTCCC ACACTTCCTT CACAAAGCTC GATAAGGGCG AATACACAAT CAGACTGCAG 
GTGCGTCACG AGAAGCGCGA CCTGCTGGAG AAGATCTCGG AGGCAAATCT GGTGGCCTCA 
TTCAAGCTGA CTAGCCCCCT CACCCTTGAT TTCTATGAGA ACTACAATCA GTGCATAGTG 
GGAGGTCGTA AATACGTCTC GAGTCCGCTA AGGCTGTCCA CTCGGGTGCT TTATATCGCT 
CCTATTACCC AAGAGCGACT TACCAAGGCC AATCTGCCCG CTCAATGCGC CTGGCTGAGC 
GGCAATCTGG TATTCCCGCA AGATGAGGTC GGACGGCGAG TGGCTCAGCA TCCATTTACT 
TACATCCTCA ATCCCGCTGA GAAAAAGTCA CACACGAATG GCTCGAGCAA CGGTTCCAGC 
GCTGCAGGAT CCACAGCTAC GGCAGCTGCT GTCACCACTG CTAATGGCGC AAAACCGAAA 
GCTCCGGCGA CTCCACAAGC GGCCACCTCA GTGACCAATC CTGCGGCCGG CGATGGAATT 
TCTGTTCAGA ACGACCCGCC TGTGGACAGC AGTGGGAGTC CCGCCTCACC CAAAAAGGGC 
AAGGCCAATG CCGACGATTA TGCCGAAAGT TTTCGCGACT TTCAATGTTC GCAGATTGTC 
AAGTGTGAAC TGGAAATGGC AGAGAAAATC TATAATGATG TAGTCGCTGC CCATCCCAAG 
CATTTGCAGG CAAACCTGCT ACTTATCCAG AACATCGAGT CCAATCAGCT GAAGTCGCAA 
CTGCCGCTGA CTTTCGTCAA TGCTCAAAAG ACATCGCCAC CAGAGGCAGG CGAGAGCGCC 
GACAAGCAAA AGGAGGATCA AAAGAAAGTA CGAAGCGCCC TGGAGCGAAT TGTTAAGCTG 
GCCGACAAGG TGATCCAGGA GACCGATTCC GAGGCACTGC TCTCCTACTA CGGTCTGAAG 
AACGACACTC GTGCCGATGC AGCCAAGATA AAGACCAACA TGGACAAACA GAAAAACACT 
CTCATTGAGG CGCTAAGCAA GAAGGGCATT GCTGTGGCAA AGCTGGCTGT ATTAGACGAC 
TGTATCAAGG ATAGCCTGGC TGAGATCAAC GAGCTGTATA CCGAGATTAT CAAGTTCGTG 
GACGCCAACG ACTCCAAGGC CATCCAGTTC GCTCTGTGGC ACGCCTATGC CCATGGCCAC 
TACGGTCGCA TGTACAAGTA TGTGGTTAAG CTAATCGAGG AGAAGCGAAC CCGCGATCAC 
TTCGTGGAGC TGGCCGCCAT CAACGGCGCC CTGGGTCACG AGCACATCCG TACTGTCATC 
AACCGTATGA TGATCACTGC CTTTCCCAGC AGCTTCCGTT TGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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TppII cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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