tpp2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	tpp2
Entrez Gene ID	100331669
Full Name	tripeptidyl peptidase 2
Synonyms	si:ch73-244f7.4
General protein information	Preferred Names tripeptidyl peptidase 2 Names tripeptidyl-peptidase 2 tripeptidyl peptidase II
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005168010.3	XP_005168067.1	tripeptidyl-peptidase 2 isoform X1
XM_002660321.5	XP_002660367.2	tripeptidyl-peptidase 2 isoform X3
XM_005168011.3	XP_005168068.1	tripeptidyl-peptidase 2 isoform X2
XM_005168010.4	XP_005168067.1	tripeptidyl-peptidase 2 isoform X1
XM_005168011.4	XP_005168068.1	tripeptidyl-peptidase 2 isoform X2
XM_002660321.6	XP_002660367.2	tripeptidyl-peptidase 2 isoform X3

Pathways from BioSystems

Homologies

	TPP2	XP_002800874.1
Mus musculus (house mouse)	Tpp2	NP_033444.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004870	XP_314316.4
Oryza sativa (rice)	Os02g0664300	NP_001047661.1
Xenopus tropicalis (tropical clawed frog)	tpp2	XP_002933059.1
Bos taurus (cattle)	TPP2	NP_001092504.1
Danio rerio (zebrafish)	tpp2	XP_005168068.1
Schizosaccharomyces pombe (fission yeast)	tpp2	NP_594951.1
Arabidopsis thaliana (thale cress)	TPP2	NP_193817.2
Pan troglodytes (chimpanzee)	TPP2	XP_001151602.1
Rattus norvegicus (Norway rat)	Tpp2	NP_112399.1
Caenorhabditis elegans (roundworm)	F21H12.6	NP_495221.1
Homo sapiens (human)	TPP2	NP_003282.2
Canis lupus familiaris (dog)	TPP2	XP_005634119.1
Gallus gallus (chicken)	TPP2	XP_004938615.1
Drosophila melanogaster (fruit fly)	TppII	NP_725252.1

Gene Ontology

The following tpp2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tpp2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa05338	XM_005168010.4 Latest version!	Danio rerio tripeptidyl peptidase 2 (tpp2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa05339	XM_005168011.4 Latest version!	Danio rerio tripeptidyl peptidase 2 (tpp2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa05340	XM_002660321.6 Latest version!	Danio rerio tripeptidyl peptidase 2 (tpp2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
View Old Accession Versions >>
ODa05338	XM_005168010.3	Danio rerio si:ch73-244f7.4 (si:ch73-244f7.4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa05339	XM_005168011.3	Danio rerio si:ch73-244f7.4 (si:ch73-244f7.4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa05340	XM_002660321.5	Danio rerio si:ch73-244f7.4 (si:ch73-244f7.4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tpp2 ( XM_005168010.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005168067.1 Danio rerio si:ch73-244f7.4 (si:ch73-244f7.4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa05338
Clone ID Related Accession (Same CDS sequence)	XM_005168010.3 , XM_005168010.4
Accession Version	XM_005168010.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3792bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	tripeptidyl-peptidase 2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005168010.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCTC ATTCCGCCGA AGAACCGTTC CCGTTTCACG GGCTGCTCCC GAAAAAAGAG 
ACCGGCGCTG CTTCGTTCCT CACTAAGTAC CCGGAGTACG ATGGCCGAGG GGTGCTCATC 
GCGATTCTCG ACACCGGCGT GGACCCAGGG GCCCCGGGCA TGCAGATCAC CACAGACGGA 
AAGCCGAAGA TCGTGGACAT CATCGACACC ACGGGCAGCG GTGACGTGAA CATGAGCACA 
GTGGTGGAGG TGAAGGAGGG CAGTGTGAGC GGTCTGACTG GACGCACGCT TAAAATTCCT 
GCAGCGTGGG TCAATCCGTC TGGGAAATAT CACATTGGAG TGAAAAACGG CTATGATTTC 
TTCCCTAAAG CTCTTAAAGA GAGAATACAG AAAGAGCGCA AGGAGAAGCT GTGGGATCCG 
ACACACCGAG CAGCTCTCGC TGAGGCCAGC CGCAGGACAG AAGAGTTCGA CCAAACACAC 
ACAAACCCCT CACAGATGGA GAAGCTCCAG AAGGAGGAGC TGCAGTGTCA TGTGGAGCTG 
CTGGGAATGC TGGAGAAGAA ATACAGTGAT CCTGGACCCG TGTATGACTG TATATCATGG 
CACGACGGGG TCACGTGGAG GGCTGTGGTG GACACGTCTG AGTGTGGAGA TCTGTCGTCC 
TGCACTGTCC TCAGCTCCTA CAGAGAGAGA CAGGAGTTCT GCACGCTGGG ATTCGCTGAG 
ATGCTCAACT TCTCCGTCAA CATCTATGAT GAGGGAAACA CACTCTGCAT CGTCACCAGC 
GGAGGGGCTC ATGGGACACA CGTGGCCAGC ATCGCAGCAG GTCATTTCCC AGATGAACCC 
GAGCGTAACG GTGTGGCTCC TGGAGCTCAG ATCCTGGCGC TGAAGATCGG AGACACTCGC 
CTGAGCACCA TGGAGACGGG AACCGGCCTC ATACGAGCGA TGATTGAAGT TATAAACTAT 
AAGTGTGATC TTGTGAACTA CAGTTACGGT GAAGCGACGC ATTGGCCAAA CTCAGGGAGA 
ATCTGTGAGG TCATCACGGA GGCTGTACAG AAGTATAATG TTATGTTCGT GTCGAGTGCG 
GGGAATAACG GTCCATGTCT GACCACCGTG GGCTGTCCTG GAGGAACCAC CAGCAGTGTG 
ATCGGAGTGG GAGCGTATGT TACTCCAGAT ATGATGGTGG CCGAGTATTC TCTGAGAGAG 
AAGCTTCCTC CTAACCAGTA CACCTGGTCA TCCCGTGGGC CCTGCACAGA CGGCGCTCTG 
GGCGTCAGTA TTAGTGCTCC GGGCGGCGCC ATCGCCTCCG TGCCCAACTG GACGCTGCGC 
GGCACTCAGC TGATGAACGG CACGTCCATG TCCTCCCCGA ACGCCTGCGG AGGAATCGCC 
CTCGTGCTGT CAGGTCTGAA GCAGAACGGT CTGCGGCCCA CGGTGCCGGC GGTGCGCAGA 
GCTCTGGAGA ACACAGCTCA GAAGGTGGAG GAGATCGAGG TGTTTGCGCA GGGACACGGA 
ATCATACAGG TGGAGAGGGC GTTCGACTAC CTCATGCAGC ACAATTCTCT GGCCTCCAGC 
AGTCTGGGCT TCAGCGTCAG TGCAGGATCT CAGAGAGGAA TCTACCTCAG AGACGCCACA 
CACGTCACTG CACCATCTGA CCACGGCGTC GGCATCGAGC CGATCTTTCC AGAGAACACC 
GAGAACGCAG CTCGTATCTC TCTGCAGCTT CACCTGGCGC TGGTCTGTAA TGCGTCCTGG 
GTTCAGTGTC CGTCCCATCT GGAGCTCATG AATCAGTGCA GGCATGTGAA TGTGCGCATC 
GACCCACAGG GCCTTCGAGA GGGACTGCAC TACACCGAGG TGTGTGGATA CGACACAACT 
GCTCTCAGCG CCGGCCCGCT CTTCAGAGTG CCCATAACCG TTATAATCCC TACCAAAGTA 
TCTGACAGTC GCAGTCAGGA GCTCTCCTTC ACTGATGTTC ACTTCCGGCC AGGACAGATC 
CGTCGCCACT TCATCACCGT CCCTCAAGGA GCATCATGGG CAGAGATCTC TCTGACGTCT 
CACACTGGCG ACGTGTCCTC GAAGTTCGTC CTGCATGCGG TTCATCTGGT GAAGCAGCGA 
GCGTATCGAG CCAACGAGTT CTACAAGTTC TCCTCTCTGC TGGAGAAAGG CTCGCTCACA 
GAGGCCTTTC CAGTGCTGCC AGGTCGGACT CTGGAGCTGT GTATTGCGCG CTGGTGGGCG 
AGTCTCGGTG ATGTCACCAT CGATTATGTC ATTTCCTTCC ACGGCCTGGT GACGTCGCCC 
TCTCCTATTC ACATCCACGC TTCTGAAGGC ATTTCTAGTT TTGAGGTTTG CTCTCCGTTG 
AGATATGAAG ATGTTTCTCC AACAATCACA CTGAAGAACT GGGTTCAGCC GCTCCGGCCT 
GTAAGTTCTA AAATCAAACC GCTGGGCATG AGAGACGTGT TGCCCAATAA CAGGCAGCTC 
TATGAGCTCG TCCTCACCTA TAATTTCCAC CAGCCGAAGA GCGGCGAGGT GACACCCAGC 
TGTCCACTGC TCTGTGAGTT ATTATACGAG TCTGAGTTCG ACAGTCAACT CTGGCTCCTG 
TTTGACCAGA ACAAGAGGCT CATGGGATCT GGAGATGCGT ACCCACACCA GTACTCTCTG 
AAGCTGGAGA AAGGCGACTA CACTGTGCGT CTGCAGGTGC GTCACGAGCA GCAGAGTGAG 
CTGGAGCGTC TGAAGGATCT GCCGTTCGTG GTGTCTCACC GTCTGTCCAA CACTCTCTCG 
CTGGACGTCT ACGAGACGCA TCGCGCCGCC CTGCTGGCCA AGAAGAAGGC CAACTCCATC 
ACACTGAGCC CCGGGGCCTC ACAGCCGTTC TACATCACCA GTCTGCCCGA CGACAAGATC 
CCTAAAGGCT CCGGTCCAGG CGGGTTCATC TCCGGCTCTC TGGTTCTGCC CAAGAGTGAG 
TTCGGCAAAA AAGCACAGGG ACAAGCTTCA GCCAAACGAC AAGGAAAATT TAAAAAGGAT 
ATCGTGCCAA TCTTTTACCA CCTGGTTCCT GCTCCCAACA AAAGCAAGAA CGGGAAGGAG 
AAGGAGAAGG ACCCGGAGAA GGAGAAGGAC GTGAAGGAGG AGTTCAGTGA GGCGCTGCGG 
GACCTGAAGA TCCAGTGGAT GAGCAAACTG GACAACAGTT CACTGTATGA GGAGCTGAAG 
GAGGCGTTTC CTACACACCT GCCACTGCAT GTGCAGAGAC TCCATCAGCT CGACTCTGAG 
AAGGAGCGTC TGAAGCGTCT GCCGGAGATT GTATCCGCTG CAGAGTCAGT GATGTCACAC 
ATCGATCAGA CGGCGCTGGC AGTGTACCTC ACCATGAAGA CGGACCCACG GCCCGACGCC 
GCCTCCATCA AGAGTGATAT GGAGAAGCAG AAGGCGTCTC TGCTGGATGC GCTCTGCAGG 
AAGGGCTGTG CTCTGGCGGA TCAGCTGATG CAGACGCCGC TGCAGGACGG GGCCAGTGCC 
GGACAGGTGA GCAGCTCGGA GGAGGAGTCA GTGGGCGTGG CCAAAGCTCT GACGGACACC 
TTCTGGGAGC TGCAGAAGTG GGCGGAGCTT ACAGACTCTA AGGTTTTGTC GTTCTCCTAT 
AAGCATGCGC TGGCCAATAA GATGTACGCC AGAGCCTTGA AATACGCAAT AAAGATTGTA 
GAGGACAAAC CCAGCAGAGA GAACTCCAAG AACTGCTTAC AGCTGATGCG TTCTCTGGGC 
TGGACACACT GCGTCTCGTT CACTGAGAGC TGGCTGCCGA TCCTCTTCCC GGCAGACTTC 
ACACCCTTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005168067.1
CDS	96..3887
Translation	MSAHSAEEPFPFHGLLPKKETGAASFLTKYPEYDGRGVLIAILDTGVDPGAPGMQITTDGKPKIVDIIDTTGSGDVNMSTVVEVKEGSVSGLTGRTLKIPAAWVNPSGKYHIGVKNGYDFFPKALKERIQKERKEKLWDPTHRAALAEASRRTEEFDQTHTNPSQMEKLQKEELQCHVELLGMLEKKYSDPGPVYDCISWHDGVTWRAVVDTSECGDLSSCTVLSSYRERQEFCTLGFAEMLNFSVNIYDEGNTLCIVTSGGAHGTHVASIAAGHFPDEPERNGVAPGAQILALKIGDTRLSTMETGTGLIRAMIEVINYKCDLVNYSYGEATHWPNSGRICEVITEAVQKYNVMFVSSAGNNGPCLTTVGCPGGTTSSVIGVGAYVTPDMMVAEYSLREKLPPNQYTWSSRGPCTDGALGVSISAPGGAIASVPNWTLRGTQLMNGTSMSSPNACGGIALVLSGLKQNGLRPTVPAVRRALENTAQKVEEIEVFAQGHGIIQVERAFDYLMQHNSLASSSLGFSVSAGSQRGIYLRDATHVTAPSDHGVGIEPIFPENTENAARISLQLHLALVCNASWVQCPSHLELMNQCRHVNVRIDPQGLREGLHYTEVCGYDTTALSAGPLFRVPITVIIPTKVSDSRSQELSFTDVHFRPGQIRRHFITVPQGASWAEISLTSHTGDVSSKFVLHAVHLVKQRAYRANEFYKFSSLLEKGSLTEAFPVLPGRTLELCIARWWASLGDVTIDYVISFHGLVTSPSPIHIHASEGISSFEVCSPLRYEDVSPTITLKNWVQPLRPVSSKIKPLGMRDVLPNNRQLYELVLTYNFHQPKSGEVTPSCPLLCELLYESEFDSQLWLLFDQNKRLMGSGDAYPHQYSLKLEKGDYTVRLQVRHEQQSELERLKDLPFVVSHRLSNTLSLDVYETHRAALLAKKKANSITLSPGASQPFYITSLPDDKIPKGSGPGGFISGSLVLPKSEFGKKAQGQASAKRQGKFKKDIVPIFYHLVPAPNKSKNGKEKEKDPEKEKDVKEEFSEALRDLKIQWMSKLDNSSLYEELKEAFPTHLPLHVQRLHQLDSEKERLKRLPEIVSAAESVMSHIDQTALAVYLTMKTDPRPDAASIKSDMEKQKASLLDALCRKGCALADQLMQTPLQDGASAGQVSSSEEESVGVAKALTDTFWELQKWAELTDSKVLSFSYKHALANKMYARALKYAIKIVEDKPSRENSKNCLQLMRSLGWTHCVSFTESWLPILFPADFTPF

Target ORF information:

RefSeq Version	XM_005168010.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch73-244f7.4 (si:ch73-244f7.4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005168010.3

ORF Insert Sequence:

ATGTCTGCTC ATTCCGCCGA AGAACCGTTC CCGTTTCACG GGCTGCTCCC GAAAAAAGAG 
ACCGGCGCTG CTTCGTTCCT CACTAAGTAC CCGGAGTACG ATGGCCGAGG GGTGCTCATC 
GCGATTCTCG ACACCGGCGT GGACCCAGGG GCCCCGGGCA TGCAGATCAC CACAGACGGA 
AAGCCGAAGA TCGTGGACAT CATCGACACC ACGGGCAGCG GTGACGTGAA CATGAGCACA 
GTGGTGGAGG TGAAGGAGGG CAGTGTGAGC GGTCTGACTG GACGCACGCT TAAAATTCCT 
GCAGCGTGGG TCAATCCGTC TGGGAAATAT CACATTGGAG TGAAAAACGG CTATGATTTC 
TTCCCTAAAG CTCTTAAAGA GAGAATACAG AAAGAGCGCA AGGAGAAGCT GTGGGATCCG 
ACACACCGAG CAGCTCTCGC TGAGGCCAGC CGCAGGACAG AAGAGTTCGA CCAAACACAC 
ACAAACCCCT CACAGATGGA GAAGCTCCAG AAGGAGGAGC TGCAGTGTCA TGTGGAGCTG 
CTGGGAATGC TGGAGAAGAA ATACAGTGAT CCTGGACCCG TGTATGACTG TATATCATGG 
CACGACGGGG TCACGTGGAG GGCTGTGGTG GACACGTCTG AGTGTGGAGA TCTGTCGTCC 
TGCACTGTCC TCAGCTCCTA CAGAGAGAGA CAGGAGTTCT GCACGCTGGG ATTCGCTGAG 
ATGCTCAACT TCTCCGTCAA CATCTATGAT GAGGGAAACA CACTCTGCAT CGTCACCAGC 
GGAGGGGCTC ATGGGACACA CGTGGCCAGC ATCGCAGCAG GTCATTTCCC AGATGAACCC 
GAGCGTAACG GTGTGGCTCC TGGAGCTCAG ATCCTGGCGC TGAAGATCGG AGACACTCGC 
CTGAGCACCA TGGAGACGGG AACCGGCCTC ATACGAGCGA TGATTGAAGT TATAAACTAT 
AAGTGTGATC TTGTGAACTA CAGTTACGGT GAAGCGACGC ATTGGCCAAA CTCAGGGAGA 
ATCTGTGAGG TCATCACGGA GGCTGTACAG AAGTATAATG TTATGTTCGT GTCGAGTGCG 
GGGAATAACG GTCCATGTCT GACCACCGTG GGCTGTCCTG GAGGAACCAC CAGCAGTGTG 
ATCGGAGTGG GAGCGTATGT TACTCCAGAT ATGATGGTGG CCGAGTATTC TCTGAGAGAG 
AAGCTTCCTC CTAACCAGTA CACCTGGTCA TCCCGTGGGC CCTGCACAGA CGGCGCTCTG 
GGCGTCAGTA TTAGTGCTCC GGGCGGCGCC ATCGCCTCCG TGCCCAACTG GACGCTGCGC 
GGCACTCAGC TGATGAACGG CACGTCCATG TCCTCCCCGA ACGCCTGCGG AGGAATCGCC 
CTCGTGCTGT CAGGTCTGAA GCAGAACGGT CTGCGGCCCA CGGTGCCGGC GGTGCGCAGA 
GCTCTGGAGA ACACAGCTCA GAAGGTGGAG GAGATCGAGG TGTTTGCGCA GGGACACGGA 
ATCATACAGG TGGAGAGGGC GTTCGACTAC CTCATGCAGC ACAATTCTCT GGCCTCCAGC 
AGTCTGGGCT TCAGCGTCAG TGCAGGATCT CAGAGAGGAA TCTACCTCAG AGACGCCACA 
CACGTCACTG CACCATCTGA CCACGGCGTC GGCATCGAGC CGATCTTTCC AGAGAACACC 
GAGAACGCAG CTCGTATCTC TCTGCAGCTT CACCTGGCGC TGGTCTGTAA TGCGTCCTGG 
GTTCAGTGTC CGTCCCATCT GGAGCTCATG AATCAGTGCA GGCATGTGAA TGTGCGCATC 
GACCCACAGG GCCTTCGAGA GGGACTGCAC TACACCGAGG TGTGTGGATA CGACACAACT 
GCTCTCAGCG CCGGCCCGCT CTTCAGAGTG CCCATAACCG TTATAATCCC TACCAAAGTA 
TCTGACAGTC GCAGTCAGGA GCTCTCCTTC ACTGATGTTC ACTTCCGGCC AGGACAGATC 
CGTCGCCACT TCATCACCGT CCCTCAAGGA GCATCATGGG CAGAGATCTC TCTGACGTCT 
CACACTGGCG ACGTGTCCTC GAAGTTCGTC CTGCATGCGG TTCATCTGGT GAAGCAGCGA 
GCGTATCGAG CCAACGAGTT CTACAAGTTC TCCTCTCTGC TGGAGAAAGG CTCGCTCACA 
GAGGCCTTTC CAGTGCTGCC AGGTCGGACT CTGGAGCTGT GTATTGCGCG CTGGTGGGCG 
AGTCTCGGTG ATGTCACCAT CGATTATGTC ATTTCCTTCC ACGGCCTGGT GACGTCGCCC 
TCTCCTATTC ACATCCACGC TTCTGAAGGC ATTTCTAGTT TTGAGGTTTG CTCTCCGTTG 
AGATATGAAG ATGTTTCTCC AACAATCACA CTGAAGAACT GGGTTCAGCC GCTCCGGCCT 
GTAAGTTCTA AAATCAAACC GCTGGGCATG AGAGACGTGT TGCCCAATAA CAGGCAGCTC 
TATGAGCTCG TCCTCACCTA TAATTTCCAC CAGCCGAAGA GCGGCGAGGT GACACCCAGC 
TGTCCACTGC TCTGTGAGTT ATTATACGAG TCTGAGTTCG ACAGTCAACT CTGGCTCCTG 
TTTGACCAGA ACAAGAGGCT CATGGGATCT GGAGATGCGT ACCCACACCA GTACTCTCTG 
AAGCTGGAGA AAGGCGACTA CACTGTGCGT CTGCAGGTGC GTCACGAGCA GCAGAGTGAG 
CTGGAGCGTC TGAAGGATCT GCCGTTCGTG GTGTCTCACC GTCTGTCCAA CACTCTCTCG 
CTGGACGTCT ACGAGACGCA TCGCGCCGCC CTGCTGGCCA AGAAGAAGGC CAACTCCATC 
ACACTGAGCC CCGGGGCCTC ACAGCCGTTC TACATCACCA GTCTGCCCGA CGACAAGATC 
CCTAAAGGCT CCGGTCCAGG CGGGTTCATC TCCGGCTCTC TGGTTCTGCC CAAGAGTGAG 
TTCGGCAAAA AAGCACAGGG ACAAGCTTCA GCCAAACGAC AAGGAAAATT TAAAAAGGAT 
ATCGTGCCAA TCTTTTACCA CCTGGTTCCT GCTCCCAACA AAAGCAAGAA CGGGAAGGAG 
AAGGAGAAGG ACCCGGAGAA GGAGAAGGAC GTGAAGGAGG AGTTCAGTGA GGCGCTGCGG 
GACCTGAAGA TCCAGTGGAT GAGCAAACTG GACAACAGTT CACTGTATGA GGAGCTGAAG 
GAGGCGTTTC CTACACACCT GCCACTGCAT GTGCAGAGAC TCCATCAGCT CGACTCTGAG 
AAGGAGCGTC TGAAGCGTCT GCCGGAGATT GTATCCGCTG CAGAGTCAGT GATGTCACAC 
ATCGATCAGA CGGCGCTGGC AGTGTACCTC ACCATGAAGA CGGACCCACG GCCCGACGCC 
GCCTCCATCA AGAGTGATAT GGAGAAGCAG AAGGCGTCTC TGCTGGATGC GCTCTGCAGG 
AAGGGCTGTG CTCTGGCGGA TCAGCTGATG CAGACGCCGC TGCAGGACGG GGCCAGTGCC 
GGACAGGTGA GCAGCTCGGA GGAGGAGTCA GTGGGCGTGG CCAAAGCTCT GACGGACACC 
TTCTGGGAGC TGCAGAAGTG GGCGGAGCTT ACAGACTCTA AGGTTTTGTC GTTCTCCTAT 
AAGCATGCGC TGGCCAATAA GATGTACGCC AGAGCCTTGA AATACGCAAT AAAGATTGTA 
GAGGACAAAC CCAGCAGAGA GAACTCCAAG AACTGCTTAC AGCTGATGCG TTCTCTGGGC 
TGGACACACT GCGTCTCGTT CACTGAGAGC TGGCTGCCGA TCCTCTTCCC GGCAGACTTC 
ACACCCTTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tpp2 ( XM_005168010.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005168067.1 Danio rerio tripeptidyl peptidase 2 (tpp2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa05338
Clone ID Related Accession (Same CDS sequence)	XM_005168010.3 , XM_005168010.4
Accession Version	XM_005168010.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3792bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tripeptidyl-peptidase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005168010.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCTC ATTCCGCCGA AGAACCGTTC CCGTTTCACG GGCTGCTCCC GAAAAAAGAG 
ACCGGCGCTG CTTCGTTCCT CACTAAGTAC CCGGAGTACG ATGGCCGAGG GGTGCTCATC 
GCGATTCTCG ACACCGGCGT GGACCCAGGG GCCCCGGGCA TGCAGATCAC CACAGACGGA 
AAGCCGAAGA TCGTGGACAT CATCGACACC ACGGGCAGCG GTGACGTGAA CATGAGCACA 
GTGGTGGAGG TGAAGGAGGG CAGTGTGAGC GGTCTGACTG GACGCACGCT TAAAATTCCT 
GCAGCGTGGG TCAATCCGTC TGGGAAATAT CACATTGGAG TGAAAAACGG CTATGATTTC 
TTCCCTAAAG CTCTTAAAGA GAGAATACAG AAAGAGCGCA AGGAGAAGCT GTGGGATCCG 
ACACACCGAG CAGCTCTCGC TGAGGCCAGC CGCAGGACAG AAGAGTTCGA CCAAACACAC 
ACAAACCCCT CACAGATGGA GAAGCTCCAG AAGGAGGAGC TGCAGTGTCA TGTGGAGCTG 
CTGGGAATGC TGGAGAAGAA ATACAGTGAT CCTGGACCCG TGTATGACTG TATATCATGG 
CACGACGGGG TCACGTGGAG GGCTGTGGTG GACACGTCTG AGTGTGGAGA TCTGTCGTCC 
TGCACTGTCC TCAGCTCCTA CAGAGAGAGA CAGGAGTTCT GCACGCTGGG ATTCGCTGAG 
ATGCTCAACT TCTCCGTCAA CATCTATGAT GAGGGAAACA CACTCTGCAT CGTCACCAGC 
GGAGGGGCTC ATGGGACACA CGTGGCCAGC ATCGCAGCAG GTCATTTCCC AGATGAACCC 
GAGCGTAACG GTGTGGCTCC TGGAGCTCAG ATCCTGGCGC TGAAGATCGG AGACACTCGC 
CTGAGCACCA TGGAGACGGG AACCGGCCTC ATACGAGCGA TGATTGAAGT TATAAACTAT 
AAGTGTGATC TTGTGAACTA CAGTTACGGT GAAGCGACGC ATTGGCCAAA CTCAGGGAGA 
ATCTGTGAGG TCATCACGGA GGCTGTACAG AAGTATAATG TTATGTTCGT GTCGAGTGCG 
GGGAATAACG GTCCATGTCT GACCACCGTG GGCTGTCCTG GAGGAACCAC CAGCAGTGTG 
ATCGGAGTGG GAGCGTATGT TACTCCAGAT ATGATGGTGG CCGAGTATTC TCTGAGAGAG 
AAGCTTCCTC CTAACCAGTA CACCTGGTCA TCCCGTGGGC CCTGCACAGA CGGCGCTCTG 
GGCGTCAGTA TTAGTGCTCC GGGCGGCGCC ATCGCCTCCG TGCCCAACTG GACGCTGCGC 
GGCACTCAGC TGATGAACGG CACGTCCATG TCCTCCCCGA ACGCCTGCGG AGGAATCGCC 
CTCGTGCTGT CAGGTCTGAA GCAGAACGGT CTGCGGCCCA CGGTGCCGGC GGTGCGCAGA 
GCTCTGGAGA ACACAGCTCA GAAGGTGGAG GAGATCGAGG TGTTTGCGCA GGGACACGGA 
ATCATACAGG TGGAGAGGGC GTTCGACTAC CTCATGCAGC ACAATTCTCT GGCCTCCAGC 
AGTCTGGGCT TCAGCGTCAG TGCAGGATCT CAGAGAGGAA TCTACCTCAG AGACGCCACA 
CACGTCACTG CACCATCTGA CCACGGCGTC GGCATCGAGC CGATCTTTCC AGAGAACACC 
GAGAACGCAG CTCGTATCTC TCTGCAGCTT CACCTGGCGC TGGTCTGTAA TGCGTCCTGG 
GTTCAGTGTC CGTCCCATCT GGAGCTCATG AATCAGTGCA GGCATGTGAA TGTGCGCATC 
GACCCACAGG GCCTTCGAGA GGGACTGCAC TACACCGAGG TGTGTGGATA CGACACAACT 
GCTCTCAGCG CCGGCCCGCT CTTCAGAGTG CCCATAACCG TTATAATCCC TACCAAAGTA 
TCTGACAGTC GCAGTCAGGA GCTCTCCTTC ACTGATGTTC ACTTCCGGCC AGGACAGATC 
CGTCGCCACT TCATCACCGT CCCTCAAGGA GCATCATGGG CAGAGATCTC TCTGACGTCT 
CACACTGGCG ACGTGTCCTC GAAGTTCGTC CTGCATGCGG TTCATCTGGT GAAGCAGCGA 
GCGTATCGAG CCAACGAGTT CTACAAGTTC TCCTCTCTGC TGGAGAAAGG CTCGCTCACA 
GAGGCCTTTC CAGTGCTGCC AGGTCGGACT CTGGAGCTGT GTATTGCGCG CTGGTGGGCG 
AGTCTCGGTG ATGTCACCAT CGATTATGTC ATTTCCTTCC ACGGCCTGGT GACGTCGCCC 
TCTCCTATTC ACATCCACGC TTCTGAAGGC ATTTCTAGTT TTGAGGTTTG CTCTCCGTTG 
AGATATGAAG ATGTTTCTCC AACAATCACA CTGAAGAACT GGGTTCAGCC GCTCCGGCCT 
GTAAGTTCTA AAATCAAACC GCTGGGCATG AGAGACGTGT TGCCCAATAA CAGGCAGCTC 
TATGAGCTCG TCCTCACCTA TAATTTCCAC CAGCCGAAGA GCGGCGAGGT GACACCCAGC 
TGTCCACTGC TCTGTGAGTT ATTATACGAG TCTGAGTTCG ACAGTCAACT CTGGCTCCTG 
TTTGACCAGA ACAAGAGGCT CATGGGATCT GGAGATGCGT ACCCACACCA GTACTCTCTG 
AAGCTGGAGA AAGGCGACTA CACTGTGCGT CTGCAGGTGC GTCACGAGCA GCAGAGTGAG 
CTGGAGCGTC TGAAGGATCT GCCGTTCGTG GTGTCTCACC GTCTGTCCAA CACTCTCTCG 
CTGGACGTCT ACGAGACGCA TCGCGCCGCC CTGCTGGCCA AGAAGAAGGC CAACTCCATC 
ACACTGAGCC CCGGGGCCTC ACAGCCGTTC TACATCACCA GTCTGCCCGA CGACAAGATC 
CCTAAAGGCT CCGGTCCAGG CGGGTTCATC TCCGGCTCTC TGGTTCTGCC CAAGAGTGAG 
TTCGGCAAAA AAGCACAGGG ACAAGCTTCA GCCAAACGAC AAGGAAAATT TAAAAAGGAT 
ATCGTGCCAA TCTTTTACCA CCTGGTTCCT GCTCCCAACA AAAGCAAGAA CGGGAAGGAG 
AAGGAGAAGG ACCCGGAGAA GGAGAAGGAC GTGAAGGAGG AGTTCAGTGA GGCGCTGCGG 
GACCTGAAGA TCCAGTGGAT GAGCAAACTG GACAACAGTT CACTGTATGA GGAGCTGAAG 
GAGGCGTTTC CTACACACCT GCCACTGCAT GTGCAGAGAC TCCATCAGCT CGACTCTGAG 
AAGGAGCGTC TGAAGCGTCT GCCGGAGATT GTATCCGCTG CAGAGTCAGT GATGTCACAC 
ATCGATCAGA CGGCGCTGGC AGTGTACCTC ACCATGAAGA CGGACCCACG GCCCGACGCC 
GCCTCCATCA AGAGTGATAT GGAGAAGCAG AAGGCGTCTC TGCTGGATGC GCTCTGCAGG 
AAGGGCTGTG CTCTGGCGGA TCAGCTGATG CAGACGCCGC TGCAGGACGG GGCCAGTGCC 
GGACAGGTGA GCAGCTCGGA GGAGGAGTCA GTGGGCGTGG CCAAAGCTCT GACGGACACC 
TTCTGGGAGC TGCAGAAGTG GGCGGAGCTT ACAGACTCTA AGGTTTTGTC GTTCTCCTAT 
AAGCATGCGC TGGCCAATAA GATGTACGCC AGAGCCTTGA AATACGCAAT AAAGATTGTA 
GAGGACAAAC CCAGCAGAGA GAACTCCAAG AACTGCTTAC AGCTGATGCG TTCTCTGGGC 
TGGACACACT GCGTCTCGTT CACTGAGAGC TGGCTGCCGA TCCTCTTCCC GGCAGACTTC 
ACACCCTTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005168067.1
CDS	92..3883
Translation	MSAHSAEEPFPFHGLLPKKETGAASFLTKYPEYDGRGVLIAILDTGVDPGAPGMQITTDGKPKIVDIIDTTGSGDVNMSTVVEVKEGSVSGLTGRTLKIPAAWVNPSGKYHIGVKNGYDFFPKALKERIQKERKEKLWDPTHRAALAEASRRTEEFDQTHTNPSQMEKLQKEELQCHVELLGMLEKKYSDPGPVYDCISWHDGVTWRAVVDTSECGDLSSCTVLSSYRERQEFCTLGFAEMLNFSVNIYDEGNTLCIVTSGGAHGTHVASIAAGHFPDEPERNGVAPGAQILALKIGDTRLSTMETGTGLIRAMIEVINYKCDLVNYSYGEATHWPNSGRICEVITEAVQKYNVMFVSSAGNNGPCLTTVGCPGGTTSSVIGVGAYVTPDMMVAEYSLREKLPPNQYTWSSRGPCTDGALGVSISAPGGAIASVPNWTLRGTQLMNGTSMSSPNACGGIALVLSGLKQNGLRPTVPAVRRALENTAQKVEEIEVFAQGHGIIQVERAFDYLMQHNSLASSSLGFSVSAGSQRGIYLRDATHVTAPSDHGVGIEPIFPENTENAARISLQLHLALVCNASWVQCPSHLELMNQCRHVNVRIDPQGLREGLHYTEVCGYDTTALSAGPLFRVPITVIIPTKVSDSRSQELSFTDVHFRPGQIRRHFITVPQGASWAEISLTSHTGDVSSKFVLHAVHLVKQRAYRANEFYKFSSLLEKGSLTEAFPVLPGRTLELCIARWWASLGDVTIDYVISFHGLVTSPSPIHIHASEGISSFEVCSPLRYEDVSPTITLKNWVQPLRPVSSKIKPLGMRDVLPNNRQLYELVLTYNFHQPKSGEVTPSCPLLCELLYESEFDSQLWLLFDQNKRLMGSGDAYPHQYSLKLEKGDYTVRLQVRHEQQSELERLKDLPFVVSHRLSNTLSLDVYETHRAALLAKKKANSITLSPGASQPFYITSLPDDKIPKGSGPGGFISGSLVLPKSEFGKKAQGQASAKRQGKFKKDIVPIFYHLVPAPNKSKNGKEKEKDPEKEKDVKEEFSEALRDLKIQWMSKLDNSSLYEELKEAFPTHLPLHVQRLHQLDSEKERLKRLPEIVSAAESVMSHIDQTALAVYLTMKTDPRPDAASIKSDMEKQKASLLDALCRKGCALADQLMQTPLQDGASAGQVSSSEEESVGVAKALTDTFWELQKWAELTDSKVLSFSYKHALANKMYARALKYAIKIVEDKPSRENSKNCLQLMRSLGWTHCVSFTESWLPILFPADFTPF

Target ORF information:

RefSeq Version	XM_005168010.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tripeptidyl peptidase 2 (tpp2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005168010.4

ORF Insert Sequence:

ATGTCTGCTC ATTCCGCCGA AGAACCGTTC CCGTTTCACG GGCTGCTCCC GAAAAAAGAG 
ACCGGCGCTG CTTCGTTCCT CACTAAGTAC CCGGAGTACG ATGGCCGAGG GGTGCTCATC 
GCGATTCTCG ACACCGGCGT GGACCCAGGG GCCCCGGGCA TGCAGATCAC CACAGACGGA 
AAGCCGAAGA TCGTGGACAT CATCGACACC ACGGGCAGCG GTGACGTGAA CATGAGCACA 
GTGGTGGAGG TGAAGGAGGG CAGTGTGAGC GGTCTGACTG GACGCACGCT TAAAATTCCT 
GCAGCGTGGG TCAATCCGTC TGGGAAATAT CACATTGGAG TGAAAAACGG CTATGATTTC 
TTCCCTAAAG CTCTTAAAGA GAGAATACAG AAAGAGCGCA AGGAGAAGCT GTGGGATCCG 
ACACACCGAG CAGCTCTCGC TGAGGCCAGC CGCAGGACAG AAGAGTTCGA CCAAACACAC 
ACAAACCCCT CACAGATGGA GAAGCTCCAG AAGGAGGAGC TGCAGTGTCA TGTGGAGCTG 
CTGGGAATGC TGGAGAAGAA ATACAGTGAT CCTGGACCCG TGTATGACTG TATATCATGG 
CACGACGGGG TCACGTGGAG GGCTGTGGTG GACACGTCTG AGTGTGGAGA TCTGTCGTCC 
TGCACTGTCC TCAGCTCCTA CAGAGAGAGA CAGGAGTTCT GCACGCTGGG ATTCGCTGAG 
ATGCTCAACT TCTCCGTCAA CATCTATGAT GAGGGAAACA CACTCTGCAT CGTCACCAGC 
GGAGGGGCTC ATGGGACACA CGTGGCCAGC ATCGCAGCAG GTCATTTCCC AGATGAACCC 
GAGCGTAACG GTGTGGCTCC TGGAGCTCAG ATCCTGGCGC TGAAGATCGG AGACACTCGC 
CTGAGCACCA TGGAGACGGG AACCGGCCTC ATACGAGCGA TGATTGAAGT TATAAACTAT 
AAGTGTGATC TTGTGAACTA CAGTTACGGT GAAGCGACGC ATTGGCCAAA CTCAGGGAGA 
ATCTGTGAGG TCATCACGGA GGCTGTACAG AAGTATAATG TTATGTTCGT GTCGAGTGCG 
GGGAATAACG GTCCATGTCT GACCACCGTG GGCTGTCCTG GAGGAACCAC CAGCAGTGTG 
ATCGGAGTGG GAGCGTATGT TACTCCAGAT ATGATGGTGG CCGAGTATTC TCTGAGAGAG 
AAGCTTCCTC CTAACCAGTA CACCTGGTCA TCCCGTGGGC CCTGCACAGA CGGCGCTCTG 
GGCGTCAGTA TTAGTGCTCC GGGCGGCGCC ATCGCCTCCG TGCCCAACTG GACGCTGCGC 
GGCACTCAGC TGATGAACGG CACGTCCATG TCCTCCCCGA ACGCCTGCGG AGGAATCGCC 
CTCGTGCTGT CAGGTCTGAA GCAGAACGGT CTGCGGCCCA CGGTGCCGGC GGTGCGCAGA 
GCTCTGGAGA ACACAGCTCA GAAGGTGGAG GAGATCGAGG TGTTTGCGCA GGGACACGGA 
ATCATACAGG TGGAGAGGGC GTTCGACTAC CTCATGCAGC ACAATTCTCT GGCCTCCAGC 
AGTCTGGGCT TCAGCGTCAG TGCAGGATCT CAGAGAGGAA TCTACCTCAG AGACGCCACA 
CACGTCACTG CACCATCTGA CCACGGCGTC GGCATCGAGC CGATCTTTCC AGAGAACACC 
GAGAACGCAG CTCGTATCTC TCTGCAGCTT CACCTGGCGC TGGTCTGTAA TGCGTCCTGG 
GTTCAGTGTC CGTCCCATCT GGAGCTCATG AATCAGTGCA GGCATGTGAA TGTGCGCATC 
GACCCACAGG GCCTTCGAGA GGGACTGCAC TACACCGAGG TGTGTGGATA CGACACAACT 
GCTCTCAGCG CCGGCCCGCT CTTCAGAGTG CCCATAACCG TTATAATCCC TACCAAAGTA 
TCTGACAGTC GCAGTCAGGA GCTCTCCTTC ACTGATGTTC ACTTCCGGCC AGGACAGATC 
CGTCGCCACT TCATCACCGT CCCTCAAGGA GCATCATGGG CAGAGATCTC TCTGACGTCT 
CACACTGGCG ACGTGTCCTC GAAGTTCGTC CTGCATGCGG TTCATCTGGT GAAGCAGCGA 
GCGTATCGAG CCAACGAGTT CTACAAGTTC TCCTCTCTGC TGGAGAAAGG CTCGCTCACA 
GAGGCCTTTC CAGTGCTGCC AGGTCGGACT CTGGAGCTGT GTATTGCGCG CTGGTGGGCG 
AGTCTCGGTG ATGTCACCAT CGATTATGTC ATTTCCTTCC ACGGCCTGGT GACGTCGCCC 
TCTCCTATTC ACATCCACGC TTCTGAAGGC ATTTCTAGTT TTGAGGTTTG CTCTCCGTTG 
AGATATGAAG ATGTTTCTCC AACAATCACA CTGAAGAACT GGGTTCAGCC GCTCCGGCCT 
GTAAGTTCTA AAATCAAACC GCTGGGCATG AGAGACGTGT TGCCCAATAA CAGGCAGCTC 
TATGAGCTCG TCCTCACCTA TAATTTCCAC CAGCCGAAGA GCGGCGAGGT GACACCCAGC 
TGTCCACTGC TCTGTGAGTT ATTATACGAG TCTGAGTTCG ACAGTCAACT CTGGCTCCTG 
TTTGACCAGA ACAAGAGGCT CATGGGATCT GGAGATGCGT ACCCACACCA GTACTCTCTG 
AAGCTGGAGA AAGGCGACTA CACTGTGCGT CTGCAGGTGC GTCACGAGCA GCAGAGTGAG 
CTGGAGCGTC TGAAGGATCT GCCGTTCGTG GTGTCTCACC GTCTGTCCAA CACTCTCTCG 
CTGGACGTCT ACGAGACGCA TCGCGCCGCC CTGCTGGCCA AGAAGAAGGC CAACTCCATC 
ACACTGAGCC CCGGGGCCTC ACAGCCGTTC TACATCACCA GTCTGCCCGA CGACAAGATC 
CCTAAAGGCT CCGGTCCAGG CGGGTTCATC TCCGGCTCTC TGGTTCTGCC CAAGAGTGAG 
TTCGGCAAAA AAGCACAGGG ACAAGCTTCA GCCAAACGAC AAGGAAAATT TAAAAAGGAT 
ATCGTGCCAA TCTTTTACCA CCTGGTTCCT GCTCCCAACA AAAGCAAGAA CGGGAAGGAG 
AAGGAGAAGG ACCCGGAGAA GGAGAAGGAC GTGAAGGAGG AGTTCAGTGA GGCGCTGCGG 
GACCTGAAGA TCCAGTGGAT GAGCAAACTG GACAACAGTT CACTGTATGA GGAGCTGAAG 
GAGGCGTTTC CTACACACCT GCCACTGCAT GTGCAGAGAC TCCATCAGCT CGACTCTGAG 
AAGGAGCGTC TGAAGCGTCT GCCGGAGATT GTATCCGCTG CAGAGTCAGT GATGTCACAC 
ATCGATCAGA CGGCGCTGGC AGTGTACCTC ACCATGAAGA CGGACCCACG GCCCGACGCC 
GCCTCCATCA AGAGTGATAT GGAGAAGCAG AAGGCGTCTC TGCTGGATGC GCTCTGCAGG 
AAGGGCTGTG CTCTGGCGGA TCAGCTGATG CAGACGCCGC TGCAGGACGG GGCCAGTGCC 
GGACAGGTGA GCAGCTCGGA GGAGGAGTCA GTGGGCGTGG CCAAAGCTCT GACGGACACC 
TTCTGGGAGC TGCAGAAGTG GGCGGAGCTT ACAGACTCTA AGGTTTTGTC GTTCTCCTAT 
AAGCATGCGC TGGCCAATAA GATGTACGCC AGAGCCTTGA AATACGCAAT AAAGATTGTA 
GAGGACAAAC CCAGCAGAGA GAACTCCAAG AACTGCTTAC AGCTGATGCG TTCTCTGGGC 
TGGACACACT GCGTCTCGTT CACTGAGAGC TGGCTGCCGA TCCTCTTCCC GGCAGACTTC 
ACACCCTTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tpp2 ( XM_005168011.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005168068.1 Danio rerio si:ch73-244f7.4 (si:ch73-244f7.4), transcript variant X2, mRNA.

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CloneID	ODa05339
Clone ID Related Accession (Same CDS sequence)	XM_005168011.3 , XM_005168011.4
Accession Version	XM_005168011.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3789bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	tripeptidyl-peptidase 2 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005168011.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCTC ATTCCGCCGA AGAACCGTTC CCGTTTCACG GGCTGCTCCC GAAAAAAGAG 
ACCGGCGCTG CTTCGTTCCT CACTAAGTAC CCGGAGTACG ATGGCCGAGG GGTGCTCATC 
GCGATTCTCG ACACCGGCGT GGACCCAGGG GCCCCGGGCA TGCAGATCAC CACAGACGGA 
AAGCCGAAGA TCGTGGACAT CATCGACACC ACGGGCAGCG GTGACGTGAA CATGAGCACA 
GTGGTGGAGG TGAAGGAGGG CAGTGTGAGC GGTCTGACTG GACGCACGCT TAAAATTCCT 
GCAGCGTGGG TCAATCCGTC TGGGAAATAT CACATTGGAG TGAAAAACGG CTATGATTTC 
TTCCCTAAAG CTCTTAAAGA GAGAATACAG AAAGAGCGCA AGGAGAAGCT GTGGGATCCG 
ACACACCGAG CAGCTCTCGC TGAGGCCAGC CGCAGGACAG AAGAGTTCGA CCAAACACAC 
ACAAACCCCT CACAGATGGA GAAGCTCCAG AAGGAGGAGC TGCAGTGTCA TGTGGAGCTG 
CTGGGAATGC TGGAGAAGAA ATACAGTGAT CCTGGACCCG TGTATGACTG TATATCATGG 
CACGACGGGG TCACGTGGAG GGCTGTGGTG GACACGTCTG AGTGTGGAGA TCTGTCGTCC 
TGCACTGTCC TCAGCTCCTA CAGAGAGAGA CAGGAGTTCT GCACGCTGGG ATTCGCTGAG 
ATGCTCAACT TCTCCGTCAA CATCTATGAT GAGGGAAACA CACTCTGCAT CGTCACCAGC 
GGAGGGGCTC ATGGGACACA CGTGGCCAGC ATCGCAGCAG GTCATTTCCC AGATGAACCC 
GAGCGTAACG GTGTGGCTCC TGGAGCTCAG ATCCTGGCGC TGAAGATCGG AGACACTCGC 
CTGAGCACCA TGGAGACGGG AACCGGCCTC ATACGAGCGA TGATTGAAGT TATAAACTAT 
AAGTGTGATC TTGTGAACTA CAGTTACGGT GAAGCGACGC ATTGGCCAAA CTCAGGGAGA 
ATCTGTGAGG TCATCACGGA GGCTGTACAG AAGTATAATG TTATGTTCGT GTCGAGTGCG 
GGGAATAACG GTCCATGTCT GACCACCGTG GGCTGTCCTG GAGGAACCAC CAGCAGTGTG 
ATCGGAGTGG GAGCGTATGT TACTCCAGAT ATGATGGTGG CCGAGTATTC TCTGAGAGAG 
AAGCTTCCTC CTAACCAGTA CACCTGGTCA TCCCGTGGGC CCTGCACAGA CGGCGCTCTG 
GGCGTCAGTA TTAGTGCTCC GGGCGGCGCC ATCGCCTCCG TGCCCAACTG GACGCTGCGC 
GGCACTCAGC TGATGAACGG CACGTCCATG TCCTCCCCGA ACGCCTGCGG AGGAATCGCC 
CTCGTGCTGT CAGGTCTGAA GCAGAACGGT CTGCGGCCCA CGGTGCCGGC GGTGCGCAGA 
GCTCTGGAGA ACACAGCTCA GAAGGTGGAG GAGATCGAGG TGTTTGCGCA GGGACACGGA 
ATCATACAGG TGGAGAGGGC GTTCGACTAC CTCATGCAGC ACAATTCTCT GGCCTCCAGC 
AGTCTGGGCT TCAGCGTCAG TGCAGGATCT CAGAGAGGAA TCTACCTCAG AGACGCCACA 
CACGTCACTG CACCATCTGA CCACGGCGTC GGCATCGAGC CGATCTTTCC AGAGAACACC 
GAGAACGCAG CTCGTATCTC TCTGCAGCTT CACCTGGCGC TGGTCTGTAA TGCGTCCTGG 
GTTCAGTGTC CGTCCCATCT GGAGCTCATG AATCAGTGCA GGCATGTGAA TGTGCGCATC 
GACCCACAGG GCCTTCGAGA GGGACTGCAC TACACCGAGG TGTGTGGATA CGACACAACT 
GCTCTCAGCG CCGGCCCGCT CTTCAGAGTG CCCATAACCG TTATAATCCC TACCAAAGTA 
TCTGACAGTC GCAGTCAGGA GCTCTCCTTC ACTGATGTTC ACTTCCGGCC AGGACAGATC 
CGTCGCCACT TCATCACCGT CCCTCAAGGA GCATCATGGG CAGAGATCTC TCTGACGTCT 
CACACTGGCG ACGTGTCCTC GAAGTTCGTC CTGCATGCGG TTCATCTGGT GAAGCAGCGA 
GCGTATCGAG CCAACGAGTT CTACAAGTTC TCCTCTCTGC TGGAGAAAGG CTCGCTCACA 
GAGGCCTTTC CAGTGCTGCC AGGTCGGACT CTGGAGCTGT GTATTGCGCG CTGGTGGGCG 
AGTCTCGGTG ATGTCACCAT CGATTATGTC ATTTCCTTCC ACGGCCTGGT GACGTCGCCC 
TCTCCTATTC ACATCCACGC TTCTGAAGGC ATTTCTAGTT TTGAGGTTTG CTCTCCGTTG 
AGATATGAAG ATGTTTCTCC AACAATCACA CTGAAGAACT GGGTTCAGCC GCTCCGGCCT 
GTAAGTTCTA AAATCAAACC GCTGGGCATG AGAGACGTGT TGCCCAATAA CAGGCAGCTC 
TATGAGCTCG TCCTCACCTA TAATTTCCAC CAGCCGAAGA GCGGCGAGGT GACACCCAGC 
TGTCCACTGC TCTGTGAGTT ATTATACGAG TCTGAGTTCG ACAGTCAACT CTGGCTCCTG 
TTTGACCAGA ACAAGAGGCT CATGGGATCT GGAGATGCGT ACCCACACCA GTACTCTCTG 
AAGCTGGAGA AAGGCGACTA CACTGTGCGT CTGCAGGTGC GTCACGAGCA GCAGAGTGAG 
CTGGAGCGTC TGAAGGATCT GCCGTTCGTG GTGTCTCACC GTCTGTCCAA CACTCTCTCG 
CTGGACGTCT ACGAGACGCA TCGCGCCGCC CTGCTGGCCA AGAAGAAGGC CAACTCCATC 
ACACTGAGCC CCGGGGCCTC ACAGCCGTTC TACATCACCA GTCTGCCCGA CGACAAGATC 
CCTAAAGGCT CCGGTCCAGG CGGGTTCATC TCCGGCTCTC TGGTTCTGCC CAAGAGTGAG 
TTCGGCAAAA AAGCAGGACA AGCTTCAGCC AAACGACAAG GAAAATTTAA AAAGGATATC 
GTGCCAATCT TTTACCACCT GGTTCCTGCT CCCAACAAAA GCAAGAACGG GAAGGAGAAG 
GAGAAGGACC CGGAGAAGGA GAAGGACGTG AAGGAGGAGT TCAGTGAGGC GCTGCGGGAC 
CTGAAGATCC AGTGGATGAG CAAACTGGAC AACAGTTCAC TGTATGAGGA GCTGAAGGAG 
GCGTTTCCTA CACACCTGCC ACTGCATGTG CAGAGACTCC ATCAGCTCGA CTCTGAGAAG 
GAGCGTCTGA AGCGTCTGCC GGAGATTGTA TCCGCTGCAG AGTCAGTGAT GTCACACATC 
GATCAGACGG CGCTGGCAGT GTACCTCACC ATGAAGACGG ACCCACGGCC CGACGCCGCC 
TCCATCAAGA GTGATATGGA GAAGCAGAAG GCGTCTCTGC TGGATGCGCT CTGCAGGAAG 
GGCTGTGCTC TGGCGGATCA GCTGATGCAG ACGCCGCTGC AGGACGGGGC CAGTGCCGGA 
CAGGTGAGCA GCTCGGAGGA GGAGTCAGTG GGCGTGGCCA AAGCTCTGAC GGACACCTTC 
TGGGAGCTGC AGAAGTGGGC GGAGCTTACA GACTCTAAGG TTTTGTCGTT CTCCTATAAG 
CATGCGCTGG CCAATAAGAT GTACGCCAGA GCCTTGAAAT ACGCAATAAA GATTGTAGAG 
GACAAACCCA GCAGAGAGAA CTCCAAGAAC TGCTTACAGC TGATGCGTTC TCTGGGCTGG 
ACACACTGCG TCTCGTTCAC TGAGAGCTGG CTGCCGATCC TCTTCCCGGC AGACTTCACA 
CCCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005168068.1
CDS	96..3884
Translation	MSAHSAEEPFPFHGLLPKKETGAASFLTKYPEYDGRGVLIAILDTGVDPGAPGMQITTDGKPKIVDIIDTTGSGDVNMSTVVEVKEGSVSGLTGRTLKIPAAWVNPSGKYHIGVKNGYDFFPKALKERIQKERKEKLWDPTHRAALAEASRRTEEFDQTHTNPSQMEKLQKEELQCHVELLGMLEKKYSDPGPVYDCISWHDGVTWRAVVDTSECGDLSSCTVLSSYRERQEFCTLGFAEMLNFSVNIYDEGNTLCIVTSGGAHGTHVASIAAGHFPDEPERNGVAPGAQILALKIGDTRLSTMETGTGLIRAMIEVINYKCDLVNYSYGEATHWPNSGRICEVITEAVQKYNVMFVSSAGNNGPCLTTVGCPGGTTSSVIGVGAYVTPDMMVAEYSLREKLPPNQYTWSSRGPCTDGALGVSISAPGGAIASVPNWTLRGTQLMNGTSMSSPNACGGIALVLSGLKQNGLRPTVPAVRRALENTAQKVEEIEVFAQGHGIIQVERAFDYLMQHNSLASSSLGFSVSAGSQRGIYLRDATHVTAPSDHGVGIEPIFPENTENAARISLQLHLALVCNASWVQCPSHLELMNQCRHVNVRIDPQGLREGLHYTEVCGYDTTALSAGPLFRVPITVIIPTKVSDSRSQELSFTDVHFRPGQIRRHFITVPQGASWAEISLTSHTGDVSSKFVLHAVHLVKQRAYRANEFYKFSSLLEKGSLTEAFPVLPGRTLELCIARWWASLGDVTIDYVISFHGLVTSPSPIHIHASEGISSFEVCSPLRYEDVSPTITLKNWVQPLRPVSSKIKPLGMRDVLPNNRQLYELVLTYNFHQPKSGEVTPSCPLLCELLYESEFDSQLWLLFDQNKRLMGSGDAYPHQYSLKLEKGDYTVRLQVRHEQQSELERLKDLPFVVSHRLSNTLSLDVYETHRAALLAKKKANSITLSPGASQPFYITSLPDDKIPKGSGPGGFISGSLVLPKSEFGKKAGQASAKRQGKFKKDIVPIFYHLVPAPNKSKNGKEKEKDPEKEKDVKEEFSEALRDLKIQWMSKLDNSSLYEELKEAFPTHLPLHVQRLHQLDSEKERLKRLPEIVSAAESVMSHIDQTALAVYLTMKTDPRPDAASIKSDMEKQKASLLDALCRKGCALADQLMQTPLQDGASAGQVSSSEEESVGVAKALTDTFWELQKWAELTDSKVLSFSYKHALANKMYARALKYAIKIVEDKPSRENSKNCLQLMRSLGWTHCVSFTESWLPILFPADFTPF

Target ORF information:

RefSeq Version	XM_005168011.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch73-244f7.4 (si:ch73-244f7.4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005168011.3

ORF Insert Sequence:

ATGTCTGCTC ATTCCGCCGA AGAACCGTTC CCGTTTCACG GGCTGCTCCC GAAAAAAGAG 
ACCGGCGCTG CTTCGTTCCT CACTAAGTAC CCGGAGTACG ATGGCCGAGG GGTGCTCATC 
GCGATTCTCG ACACCGGCGT GGACCCAGGG GCCCCGGGCA TGCAGATCAC CACAGACGGA 
AAGCCGAAGA TCGTGGACAT CATCGACACC ACGGGCAGCG GTGACGTGAA CATGAGCACA 
GTGGTGGAGG TGAAGGAGGG CAGTGTGAGC GGTCTGACTG GACGCACGCT TAAAATTCCT 
GCAGCGTGGG TCAATCCGTC TGGGAAATAT CACATTGGAG TGAAAAACGG CTATGATTTC 
TTCCCTAAAG CTCTTAAAGA GAGAATACAG AAAGAGCGCA AGGAGAAGCT GTGGGATCCG 
ACACACCGAG CAGCTCTCGC TGAGGCCAGC CGCAGGACAG AAGAGTTCGA CCAAACACAC 
ACAAACCCCT CACAGATGGA GAAGCTCCAG AAGGAGGAGC TGCAGTGTCA TGTGGAGCTG 
CTGGGAATGC TGGAGAAGAA ATACAGTGAT CCTGGACCCG TGTATGACTG TATATCATGG 
CACGACGGGG TCACGTGGAG GGCTGTGGTG GACACGTCTG AGTGTGGAGA TCTGTCGTCC 
TGCACTGTCC TCAGCTCCTA CAGAGAGAGA CAGGAGTTCT GCACGCTGGG ATTCGCTGAG 
ATGCTCAACT TCTCCGTCAA CATCTATGAT GAGGGAAACA CACTCTGCAT CGTCACCAGC 
GGAGGGGCTC ATGGGACACA CGTGGCCAGC ATCGCAGCAG GTCATTTCCC AGATGAACCC 
GAGCGTAACG GTGTGGCTCC TGGAGCTCAG ATCCTGGCGC TGAAGATCGG AGACACTCGC 
CTGAGCACCA TGGAGACGGG AACCGGCCTC ATACGAGCGA TGATTGAAGT TATAAACTAT 
AAGTGTGATC TTGTGAACTA CAGTTACGGT GAAGCGACGC ATTGGCCAAA CTCAGGGAGA 
ATCTGTGAGG TCATCACGGA GGCTGTACAG AAGTATAATG TTATGTTCGT GTCGAGTGCG 
GGGAATAACG GTCCATGTCT GACCACCGTG GGCTGTCCTG GAGGAACCAC CAGCAGTGTG 
ATCGGAGTGG GAGCGTATGT TACTCCAGAT ATGATGGTGG CCGAGTATTC TCTGAGAGAG 
AAGCTTCCTC CTAACCAGTA CACCTGGTCA TCCCGTGGGC CCTGCACAGA CGGCGCTCTG 
GGCGTCAGTA TTAGTGCTCC GGGCGGCGCC ATCGCCTCCG TGCCCAACTG GACGCTGCGC 
GGCACTCAGC TGATGAACGG CACGTCCATG TCCTCCCCGA ACGCCTGCGG AGGAATCGCC 
CTCGTGCTGT CAGGTCTGAA GCAGAACGGT CTGCGGCCCA CGGTGCCGGC GGTGCGCAGA 
GCTCTGGAGA ACACAGCTCA GAAGGTGGAG GAGATCGAGG TGTTTGCGCA GGGACACGGA 
ATCATACAGG TGGAGAGGGC GTTCGACTAC CTCATGCAGC ACAATTCTCT GGCCTCCAGC 
AGTCTGGGCT TCAGCGTCAG TGCAGGATCT CAGAGAGGAA TCTACCTCAG AGACGCCACA 
CACGTCACTG CACCATCTGA CCACGGCGTC GGCATCGAGC CGATCTTTCC AGAGAACACC 
GAGAACGCAG CTCGTATCTC TCTGCAGCTT CACCTGGCGC TGGTCTGTAA TGCGTCCTGG 
GTTCAGTGTC CGTCCCATCT GGAGCTCATG AATCAGTGCA GGCATGTGAA TGTGCGCATC 
GACCCACAGG GCCTTCGAGA GGGACTGCAC TACACCGAGG TGTGTGGATA CGACACAACT 
GCTCTCAGCG CCGGCCCGCT CTTCAGAGTG CCCATAACCG TTATAATCCC TACCAAAGTA 
TCTGACAGTC GCAGTCAGGA GCTCTCCTTC ACTGATGTTC ACTTCCGGCC AGGACAGATC 
CGTCGCCACT TCATCACCGT CCCTCAAGGA GCATCATGGG CAGAGATCTC TCTGACGTCT 
CACACTGGCG ACGTGTCCTC GAAGTTCGTC CTGCATGCGG TTCATCTGGT GAAGCAGCGA 
GCGTATCGAG CCAACGAGTT CTACAAGTTC TCCTCTCTGC TGGAGAAAGG CTCGCTCACA 
GAGGCCTTTC CAGTGCTGCC AGGTCGGACT CTGGAGCTGT GTATTGCGCG CTGGTGGGCG 
AGTCTCGGTG ATGTCACCAT CGATTATGTC ATTTCCTTCC ACGGCCTGGT GACGTCGCCC 
TCTCCTATTC ACATCCACGC TTCTGAAGGC ATTTCTAGTT TTGAGGTTTG CTCTCCGTTG 
AGATATGAAG ATGTTTCTCC AACAATCACA CTGAAGAACT GGGTTCAGCC GCTCCGGCCT 
GTAAGTTCTA AAATCAAACC GCTGGGCATG AGAGACGTGT TGCCCAATAA CAGGCAGCTC 
TATGAGCTCG TCCTCACCTA TAATTTCCAC CAGCCGAAGA GCGGCGAGGT GACACCCAGC 
TGTCCACTGC TCTGTGAGTT ATTATACGAG TCTGAGTTCG ACAGTCAACT CTGGCTCCTG 
TTTGACCAGA ACAAGAGGCT CATGGGATCT GGAGATGCGT ACCCACACCA GTACTCTCTG 
AAGCTGGAGA AAGGCGACTA CACTGTGCGT CTGCAGGTGC GTCACGAGCA GCAGAGTGAG 
CTGGAGCGTC TGAAGGATCT GCCGTTCGTG GTGTCTCACC GTCTGTCCAA CACTCTCTCG 
CTGGACGTCT ACGAGACGCA TCGCGCCGCC CTGCTGGCCA AGAAGAAGGC CAACTCCATC 
ACACTGAGCC CCGGGGCCTC ACAGCCGTTC TACATCACCA GTCTGCCCGA CGACAAGATC 
CCTAAAGGCT CCGGTCCAGG CGGGTTCATC TCCGGCTCTC TGGTTCTGCC CAAGAGTGAG 
TTCGGCAAAA AAGCAGGACA AGCTTCAGCC AAACGACAAG GAAAATTTAA AAAGGATATC 
GTGCCAATCT TTTACCACCT GGTTCCTGCT CCCAACAAAA GCAAGAACGG GAAGGAGAAG 
GAGAAGGACC CGGAGAAGGA GAAGGACGTG AAGGAGGAGT TCAGTGAGGC GCTGCGGGAC 
CTGAAGATCC AGTGGATGAG CAAACTGGAC AACAGTTCAC TGTATGAGGA GCTGAAGGAG 
GCGTTTCCTA CACACCTGCC ACTGCATGTG CAGAGACTCC ATCAGCTCGA CTCTGAGAAG 
GAGCGTCTGA AGCGTCTGCC GGAGATTGTA TCCGCTGCAG AGTCAGTGAT GTCACACATC 
GATCAGACGG CGCTGGCAGT GTACCTCACC ATGAAGACGG ACCCACGGCC CGACGCCGCC 
TCCATCAAGA GTGATATGGA GAAGCAGAAG GCGTCTCTGC TGGATGCGCT CTGCAGGAAG 
GGCTGTGCTC TGGCGGATCA GCTGATGCAG ACGCCGCTGC AGGACGGGGC CAGTGCCGGA 
CAGGTGAGCA GCTCGGAGGA GGAGTCAGTG GGCGTGGCCA AAGCTCTGAC GGACACCTTC 
TGGGAGCTGC AGAAGTGGGC GGAGCTTACA GACTCTAAGG TTTTGTCGTT CTCCTATAAG 
CATGCGCTGG CCAATAAGAT GTACGCCAGA GCCTTGAAAT ACGCAATAAA GATTGTAGAG 
GACAAACCCA GCAGAGAGAA CTCCAAGAAC TGCTTACAGC TGATGCGTTC TCTGGGCTGG 
ACACACTGCG TCTCGTTCAC TGAGAGCTGG CTGCCGATCC TCTTCCCGGC AGACTTCACA 
CCCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tpp2 ( XM_005168011.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005168068.1 Danio rerio tripeptidyl peptidase 2 (tpp2), transcript variant X2, mRNA.

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CloneID	ODa05339
Clone ID Related Accession (Same CDS sequence)	XM_005168011.3 , XM_005168011.4
Accession Version	XM_005168011.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3789bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tripeptidyl-peptidase 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005168011.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCTC ATTCCGCCGA AGAACCGTTC CCGTTTCACG GGCTGCTCCC GAAAAAAGAG 
ACCGGCGCTG CTTCGTTCCT CACTAAGTAC CCGGAGTACG ATGGCCGAGG GGTGCTCATC 
GCGATTCTCG ACACCGGCGT GGACCCAGGG GCCCCGGGCA TGCAGATCAC CACAGACGGA 
AAGCCGAAGA TCGTGGACAT CATCGACACC ACGGGCAGCG GTGACGTGAA CATGAGCACA 
GTGGTGGAGG TGAAGGAGGG CAGTGTGAGC GGTCTGACTG GACGCACGCT TAAAATTCCT 
GCAGCGTGGG TCAATCCGTC TGGGAAATAT CACATTGGAG TGAAAAACGG CTATGATTTC 
TTCCCTAAAG CTCTTAAAGA GAGAATACAG AAAGAGCGCA AGGAGAAGCT GTGGGATCCG 
ACACACCGAG CAGCTCTCGC TGAGGCCAGC CGCAGGACAG AAGAGTTCGA CCAAACACAC 
ACAAACCCCT CACAGATGGA GAAGCTCCAG AAGGAGGAGC TGCAGTGTCA TGTGGAGCTG 
CTGGGAATGC TGGAGAAGAA ATACAGTGAT CCTGGACCCG TGTATGACTG TATATCATGG 
CACGACGGGG TCACGTGGAG GGCTGTGGTG GACACGTCTG AGTGTGGAGA TCTGTCGTCC 
TGCACTGTCC TCAGCTCCTA CAGAGAGAGA CAGGAGTTCT GCACGCTGGG ATTCGCTGAG 
ATGCTCAACT TCTCCGTCAA CATCTATGAT GAGGGAAACA CACTCTGCAT CGTCACCAGC 
GGAGGGGCTC ATGGGACACA CGTGGCCAGC ATCGCAGCAG GTCATTTCCC AGATGAACCC 
GAGCGTAACG GTGTGGCTCC TGGAGCTCAG ATCCTGGCGC TGAAGATCGG AGACACTCGC 
CTGAGCACCA TGGAGACGGG AACCGGCCTC ATACGAGCGA TGATTGAAGT TATAAACTAT 
AAGTGTGATC TTGTGAACTA CAGTTACGGT GAAGCGACGC ATTGGCCAAA CTCAGGGAGA 
ATCTGTGAGG TCATCACGGA GGCTGTACAG AAGTATAATG TTATGTTCGT GTCGAGTGCG 
GGGAATAACG GTCCATGTCT GACCACCGTG GGCTGTCCTG GAGGAACCAC CAGCAGTGTG 
ATCGGAGTGG GAGCGTATGT TACTCCAGAT ATGATGGTGG CCGAGTATTC TCTGAGAGAG 
AAGCTTCCTC CTAACCAGTA CACCTGGTCA TCCCGTGGGC CCTGCACAGA CGGCGCTCTG 
GGCGTCAGTA TTAGTGCTCC GGGCGGCGCC ATCGCCTCCG TGCCCAACTG GACGCTGCGC 
GGCACTCAGC TGATGAACGG CACGTCCATG TCCTCCCCGA ACGCCTGCGG AGGAATCGCC 
CTCGTGCTGT CAGGTCTGAA GCAGAACGGT CTGCGGCCCA CGGTGCCGGC GGTGCGCAGA 
GCTCTGGAGA ACACAGCTCA GAAGGTGGAG GAGATCGAGG TGTTTGCGCA GGGACACGGA 
ATCATACAGG TGGAGAGGGC GTTCGACTAC CTCATGCAGC ACAATTCTCT GGCCTCCAGC 
AGTCTGGGCT TCAGCGTCAG TGCAGGATCT CAGAGAGGAA TCTACCTCAG AGACGCCACA 
CACGTCACTG CACCATCTGA CCACGGCGTC GGCATCGAGC CGATCTTTCC AGAGAACACC 
GAGAACGCAG CTCGTATCTC TCTGCAGCTT CACCTGGCGC TGGTCTGTAA TGCGTCCTGG 
GTTCAGTGTC CGTCCCATCT GGAGCTCATG AATCAGTGCA GGCATGTGAA TGTGCGCATC 
GACCCACAGG GCCTTCGAGA GGGACTGCAC TACACCGAGG TGTGTGGATA CGACACAACT 
GCTCTCAGCG CCGGCCCGCT CTTCAGAGTG CCCATAACCG TTATAATCCC TACCAAAGTA 
TCTGACAGTC GCAGTCAGGA GCTCTCCTTC ACTGATGTTC ACTTCCGGCC AGGACAGATC 
CGTCGCCACT TCATCACCGT CCCTCAAGGA GCATCATGGG CAGAGATCTC TCTGACGTCT 
CACACTGGCG ACGTGTCCTC GAAGTTCGTC CTGCATGCGG TTCATCTGGT GAAGCAGCGA 
GCGTATCGAG CCAACGAGTT CTACAAGTTC TCCTCTCTGC TGGAGAAAGG CTCGCTCACA 
GAGGCCTTTC CAGTGCTGCC AGGTCGGACT CTGGAGCTGT GTATTGCGCG CTGGTGGGCG 
AGTCTCGGTG ATGTCACCAT CGATTATGTC ATTTCCTTCC ACGGCCTGGT GACGTCGCCC 
TCTCCTATTC ACATCCACGC TTCTGAAGGC ATTTCTAGTT TTGAGGTTTG CTCTCCGTTG 
AGATATGAAG ATGTTTCTCC AACAATCACA CTGAAGAACT GGGTTCAGCC GCTCCGGCCT 
GTAAGTTCTA AAATCAAACC GCTGGGCATG AGAGACGTGT TGCCCAATAA CAGGCAGCTC 
TATGAGCTCG TCCTCACCTA TAATTTCCAC CAGCCGAAGA GCGGCGAGGT GACACCCAGC 
TGTCCACTGC TCTGTGAGTT ATTATACGAG TCTGAGTTCG ACAGTCAACT CTGGCTCCTG 
TTTGACCAGA ACAAGAGGCT CATGGGATCT GGAGATGCGT ACCCACACCA GTACTCTCTG 
AAGCTGGAGA AAGGCGACTA CACTGTGCGT CTGCAGGTGC GTCACGAGCA GCAGAGTGAG 
CTGGAGCGTC TGAAGGATCT GCCGTTCGTG GTGTCTCACC GTCTGTCCAA CACTCTCTCG 
CTGGACGTCT ACGAGACGCA TCGCGCCGCC CTGCTGGCCA AGAAGAAGGC CAACTCCATC 
ACACTGAGCC CCGGGGCCTC ACAGCCGTTC TACATCACCA GTCTGCCCGA CGACAAGATC 
CCTAAAGGCT CCGGTCCAGG CGGGTTCATC TCCGGCTCTC TGGTTCTGCC CAAGAGTGAG 
TTCGGCAAAA AAGCAGGACA AGCTTCAGCC AAACGACAAG GAAAATTTAA AAAGGATATC 
GTGCCAATCT TTTACCACCT GGTTCCTGCT CCCAACAAAA GCAAGAACGG GAAGGAGAAG 
GAGAAGGACC CGGAGAAGGA GAAGGACGTG AAGGAGGAGT TCAGTGAGGC GCTGCGGGAC 
CTGAAGATCC AGTGGATGAG CAAACTGGAC AACAGTTCAC TGTATGAGGA GCTGAAGGAG 
GCGTTTCCTA CACACCTGCC ACTGCATGTG CAGAGACTCC ATCAGCTCGA CTCTGAGAAG 
GAGCGTCTGA AGCGTCTGCC GGAGATTGTA TCCGCTGCAG AGTCAGTGAT GTCACACATC 
GATCAGACGG CGCTGGCAGT GTACCTCACC ATGAAGACGG ACCCACGGCC CGACGCCGCC 
TCCATCAAGA GTGATATGGA GAAGCAGAAG GCGTCTCTGC TGGATGCGCT CTGCAGGAAG 
GGCTGTGCTC TGGCGGATCA GCTGATGCAG ACGCCGCTGC AGGACGGGGC CAGTGCCGGA 
CAGGTGAGCA GCTCGGAGGA GGAGTCAGTG GGCGTGGCCA AAGCTCTGAC GGACACCTTC 
TGGGAGCTGC AGAAGTGGGC GGAGCTTACA GACTCTAAGG TTTTGTCGTT CTCCTATAAG 
CATGCGCTGG CCAATAAGAT GTACGCCAGA GCCTTGAAAT ACGCAATAAA GATTGTAGAG 
GACAAACCCA GCAGAGAGAA CTCCAAGAAC TGCTTACAGC TGATGCGTTC TCTGGGCTGG 
ACACACTGCG TCTCGTTCAC TGAGAGCTGG CTGCCGATCC TCTTCCCGGC AGACTTCACA 
CCCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005168068.1
CDS	92..3880
Translation	MSAHSAEEPFPFHGLLPKKETGAASFLTKYPEYDGRGVLIAILDTGVDPGAPGMQITTDGKPKIVDIIDTTGSGDVNMSTVVEVKEGSVSGLTGRTLKIPAAWVNPSGKYHIGVKNGYDFFPKALKERIQKERKEKLWDPTHRAALAEASRRTEEFDQTHTNPSQMEKLQKEELQCHVELLGMLEKKYSDPGPVYDCISWHDGVTWRAVVDTSECGDLSSCTVLSSYRERQEFCTLGFAEMLNFSVNIYDEGNTLCIVTSGGAHGTHVASIAAGHFPDEPERNGVAPGAQILALKIGDTRLSTMETGTGLIRAMIEVINYKCDLVNYSYGEATHWPNSGRICEVITEAVQKYNVMFVSSAGNNGPCLTTVGCPGGTTSSVIGVGAYVTPDMMVAEYSLREKLPPNQYTWSSRGPCTDGALGVSISAPGGAIASVPNWTLRGTQLMNGTSMSSPNACGGIALVLSGLKQNGLRPTVPAVRRALENTAQKVEEIEVFAQGHGIIQVERAFDYLMQHNSLASSSLGFSVSAGSQRGIYLRDATHVTAPSDHGVGIEPIFPENTENAARISLQLHLALVCNASWVQCPSHLELMNQCRHVNVRIDPQGLREGLHYTEVCGYDTTALSAGPLFRVPITVIIPTKVSDSRSQELSFTDVHFRPGQIRRHFITVPQGASWAEISLTSHTGDVSSKFVLHAVHLVKQRAYRANEFYKFSSLLEKGSLTEAFPVLPGRTLELCIARWWASLGDVTIDYVISFHGLVTSPSPIHIHASEGISSFEVCSPLRYEDVSPTITLKNWVQPLRPVSSKIKPLGMRDVLPNNRQLYELVLTYNFHQPKSGEVTPSCPLLCELLYESEFDSQLWLLFDQNKRLMGSGDAYPHQYSLKLEKGDYTVRLQVRHEQQSELERLKDLPFVVSHRLSNTLSLDVYETHRAALLAKKKANSITLSPGASQPFYITSLPDDKIPKGSGPGGFISGSLVLPKSEFGKKAGQASAKRQGKFKKDIVPIFYHLVPAPNKSKNGKEKEKDPEKEKDVKEEFSEALRDLKIQWMSKLDNSSLYEELKEAFPTHLPLHVQRLHQLDSEKERLKRLPEIVSAAESVMSHIDQTALAVYLTMKTDPRPDAASIKSDMEKQKASLLDALCRKGCALADQLMQTPLQDGASAGQVSSSEEESVGVAKALTDTFWELQKWAELTDSKVLSFSYKHALANKMYARALKYAIKIVEDKPSRENSKNCLQLMRSLGWTHCVSFTESWLPILFPADFTPF

Target ORF information:

RefSeq Version	XM_005168011.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tripeptidyl peptidase 2 (tpp2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005168011.4

ORF Insert Sequence:

ATGTCTGCTC ATTCCGCCGA AGAACCGTTC CCGTTTCACG GGCTGCTCCC GAAAAAAGAG 
ACCGGCGCTG CTTCGTTCCT CACTAAGTAC CCGGAGTACG ATGGCCGAGG GGTGCTCATC 
GCGATTCTCG ACACCGGCGT GGACCCAGGG GCCCCGGGCA TGCAGATCAC CACAGACGGA 
AAGCCGAAGA TCGTGGACAT CATCGACACC ACGGGCAGCG GTGACGTGAA CATGAGCACA 
GTGGTGGAGG TGAAGGAGGG CAGTGTGAGC GGTCTGACTG GACGCACGCT TAAAATTCCT 
GCAGCGTGGG TCAATCCGTC TGGGAAATAT CACATTGGAG TGAAAAACGG CTATGATTTC 
TTCCCTAAAG CTCTTAAAGA GAGAATACAG AAAGAGCGCA AGGAGAAGCT GTGGGATCCG 
ACACACCGAG CAGCTCTCGC TGAGGCCAGC CGCAGGACAG AAGAGTTCGA CCAAACACAC 
ACAAACCCCT CACAGATGGA GAAGCTCCAG AAGGAGGAGC TGCAGTGTCA TGTGGAGCTG 
CTGGGAATGC TGGAGAAGAA ATACAGTGAT CCTGGACCCG TGTATGACTG TATATCATGG 
CACGACGGGG TCACGTGGAG GGCTGTGGTG GACACGTCTG AGTGTGGAGA TCTGTCGTCC 
TGCACTGTCC TCAGCTCCTA CAGAGAGAGA CAGGAGTTCT GCACGCTGGG ATTCGCTGAG 
ATGCTCAACT TCTCCGTCAA CATCTATGAT GAGGGAAACA CACTCTGCAT CGTCACCAGC 
GGAGGGGCTC ATGGGACACA CGTGGCCAGC ATCGCAGCAG GTCATTTCCC AGATGAACCC 
GAGCGTAACG GTGTGGCTCC TGGAGCTCAG ATCCTGGCGC TGAAGATCGG AGACACTCGC 
CTGAGCACCA TGGAGACGGG AACCGGCCTC ATACGAGCGA TGATTGAAGT TATAAACTAT 
AAGTGTGATC TTGTGAACTA CAGTTACGGT GAAGCGACGC ATTGGCCAAA CTCAGGGAGA 
ATCTGTGAGG TCATCACGGA GGCTGTACAG AAGTATAATG TTATGTTCGT GTCGAGTGCG 
GGGAATAACG GTCCATGTCT GACCACCGTG GGCTGTCCTG GAGGAACCAC CAGCAGTGTG 
ATCGGAGTGG GAGCGTATGT TACTCCAGAT ATGATGGTGG CCGAGTATTC TCTGAGAGAG 
AAGCTTCCTC CTAACCAGTA CACCTGGTCA TCCCGTGGGC CCTGCACAGA CGGCGCTCTG 
GGCGTCAGTA TTAGTGCTCC GGGCGGCGCC ATCGCCTCCG TGCCCAACTG GACGCTGCGC 
GGCACTCAGC TGATGAACGG CACGTCCATG TCCTCCCCGA ACGCCTGCGG AGGAATCGCC 
CTCGTGCTGT CAGGTCTGAA GCAGAACGGT CTGCGGCCCA CGGTGCCGGC GGTGCGCAGA 
GCTCTGGAGA ACACAGCTCA GAAGGTGGAG GAGATCGAGG TGTTTGCGCA GGGACACGGA 
ATCATACAGG TGGAGAGGGC GTTCGACTAC CTCATGCAGC ACAATTCTCT GGCCTCCAGC 
AGTCTGGGCT TCAGCGTCAG TGCAGGATCT CAGAGAGGAA TCTACCTCAG AGACGCCACA 
CACGTCACTG CACCATCTGA CCACGGCGTC GGCATCGAGC CGATCTTTCC AGAGAACACC 
GAGAACGCAG CTCGTATCTC TCTGCAGCTT CACCTGGCGC TGGTCTGTAA TGCGTCCTGG 
GTTCAGTGTC CGTCCCATCT GGAGCTCATG AATCAGTGCA GGCATGTGAA TGTGCGCATC 
GACCCACAGG GCCTTCGAGA GGGACTGCAC TACACCGAGG TGTGTGGATA CGACACAACT 
GCTCTCAGCG CCGGCCCGCT CTTCAGAGTG CCCATAACCG TTATAATCCC TACCAAAGTA 
TCTGACAGTC GCAGTCAGGA GCTCTCCTTC ACTGATGTTC ACTTCCGGCC AGGACAGATC 
CGTCGCCACT TCATCACCGT CCCTCAAGGA GCATCATGGG CAGAGATCTC TCTGACGTCT 
CACACTGGCG ACGTGTCCTC GAAGTTCGTC CTGCATGCGG TTCATCTGGT GAAGCAGCGA 
GCGTATCGAG CCAACGAGTT CTACAAGTTC TCCTCTCTGC TGGAGAAAGG CTCGCTCACA 
GAGGCCTTTC CAGTGCTGCC AGGTCGGACT CTGGAGCTGT GTATTGCGCG CTGGTGGGCG 
AGTCTCGGTG ATGTCACCAT CGATTATGTC ATTTCCTTCC ACGGCCTGGT GACGTCGCCC 
TCTCCTATTC ACATCCACGC TTCTGAAGGC ATTTCTAGTT TTGAGGTTTG CTCTCCGTTG 
AGATATGAAG ATGTTTCTCC AACAATCACA CTGAAGAACT GGGTTCAGCC GCTCCGGCCT 
GTAAGTTCTA AAATCAAACC GCTGGGCATG AGAGACGTGT TGCCCAATAA CAGGCAGCTC 
TATGAGCTCG TCCTCACCTA TAATTTCCAC CAGCCGAAGA GCGGCGAGGT GACACCCAGC 
TGTCCACTGC TCTGTGAGTT ATTATACGAG TCTGAGTTCG ACAGTCAACT CTGGCTCCTG 
TTTGACCAGA ACAAGAGGCT CATGGGATCT GGAGATGCGT ACCCACACCA GTACTCTCTG 
AAGCTGGAGA AAGGCGACTA CACTGTGCGT CTGCAGGTGC GTCACGAGCA GCAGAGTGAG 
CTGGAGCGTC TGAAGGATCT GCCGTTCGTG GTGTCTCACC GTCTGTCCAA CACTCTCTCG 
CTGGACGTCT ACGAGACGCA TCGCGCCGCC CTGCTGGCCA AGAAGAAGGC CAACTCCATC 
ACACTGAGCC CCGGGGCCTC ACAGCCGTTC TACATCACCA GTCTGCCCGA CGACAAGATC 
CCTAAAGGCT CCGGTCCAGG CGGGTTCATC TCCGGCTCTC TGGTTCTGCC CAAGAGTGAG 
TTCGGCAAAA AAGCAGGACA AGCTTCAGCC AAACGACAAG GAAAATTTAA AAAGGATATC 
GTGCCAATCT TTTACCACCT GGTTCCTGCT CCCAACAAAA GCAAGAACGG GAAGGAGAAG 
GAGAAGGACC CGGAGAAGGA GAAGGACGTG AAGGAGGAGT TCAGTGAGGC GCTGCGGGAC 
CTGAAGATCC AGTGGATGAG CAAACTGGAC AACAGTTCAC TGTATGAGGA GCTGAAGGAG 
GCGTTTCCTA CACACCTGCC ACTGCATGTG CAGAGACTCC ATCAGCTCGA CTCTGAGAAG 
GAGCGTCTGA AGCGTCTGCC GGAGATTGTA TCCGCTGCAG AGTCAGTGAT GTCACACATC 
GATCAGACGG CGCTGGCAGT GTACCTCACC ATGAAGACGG ACCCACGGCC CGACGCCGCC 
TCCATCAAGA GTGATATGGA GAAGCAGAAG GCGTCTCTGC TGGATGCGCT CTGCAGGAAG 
GGCTGTGCTC TGGCGGATCA GCTGATGCAG ACGCCGCTGC AGGACGGGGC CAGTGCCGGA 
CAGGTGAGCA GCTCGGAGGA GGAGTCAGTG GGCGTGGCCA AAGCTCTGAC GGACACCTTC 
TGGGAGCTGC AGAAGTGGGC GGAGCTTACA GACTCTAAGG TTTTGTCGTT CTCCTATAAG 
CATGCGCTGG CCAATAAGAT GTACGCCAGA GCCTTGAAAT ACGCAATAAA GATTGTAGAG 
GACAAACCCA GCAGAGAGAA CTCCAAGAAC TGCTTACAGC TGATGCGTTC TCTGGGCTGG 
ACACACTGCG TCTCGTTCAC TGAGAGCTGG CTGCCGATCC TCTTCCCGGC AGACTTCACA 
CCCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tpp2 ( XM_002660321.5 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_002660367.2 Danio rerio si:ch73-244f7.4 (si:ch73-244f7.4), transcript variant X3, mRNA.

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CloneID	ODa05340
Clone ID Related Accession (Same CDS sequence)	XM_002660321.5 , XM_002660321.6
Accession Version	XM_002660321.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3750bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	tripeptidyl-peptidase 2 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_002660321.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCTC ATTCCGCCGA AGAACCGTTC CCGTTTCACG GGCTGCTCCC GAAAAAAGAG 
ACCGGCGCTG CTTCGTTCCT CACTAAGTAC CCGGAGTACG ATGGCCGAGG GGTGCTCATC 
GCGATTCTCG ACACCGGCGT GGACCCAGGG GCCCCGGGCA TGCAGATCAC CACAGACGGA 
AAGCCGAAGA TCGTGGACAT CATCGACACC ACGGGCAGCG GTGACGTGAA CATGAGCACA 
GTGGTGGAGG TGAAGGAGGG CAGTGTGAGC GGTCTGACTG GACGCACGCT TAAAATTCCT 
GCAGCGTGGG TCAATCCGTC TGGGAAATAT CACATTGGAG TGAAAAACGG CTATGATTTC 
TTCCCTAAAG CTCTTAAAGA GAGAATACAG AAAGAGCGCA AGGAGAAGCT GTGGGATCCG 
ACACACCGAG CAGCTCTCGC TGAGGCCAGC CGCAGGACAG AAGAGTTCGA CCAAACACAC 
ACAAACCCCT CACAGATGGA GAAGCTCCAG AAGGAGGAGC TGCAGTGTCA TGTGGAGCTG 
CTGGGAATGC TGGAGAAGAA ATACAGTGAT CCTGGACCCG TGTATGACTG TATATCATGG 
CACGACGGGG TCACGTGGAG GGCTGTGGTG GACACGTCTG AGTGTGGAGA TCTGTCGTCC 
TGCACTGTCC TCAGCTCCTA CAGAGAGAGA CAGGAGTTCT GCACGCTGGG ATTCGCTGAG 
ATGCTCAACT TCTCCGTCAA CATCTATGAT GAGGGAAACA CACTCTGCAT CGTCACCAGC 
GGAGGGGCTC ATGGGACACA CGTGGCCAGC ATCGCAGCAG GTCATTTCCC AGATGAACCC 
GAGCGTAACG GTGTGGCTCC TGGAGCTCAG ATCCTGGCGC TGAAGATCGG AGACACTCGC 
CTGAGCACCA TGGAGACGGG AACCGGCCTC ATACGAGCGA TGATTGAAGT TATAAACTAT 
AAGTGTGATC TTGTGAACTA CAGTTACGGT GAAGCGACGC ATTGGCCAAA CTCAGGGAGA 
ATCTGTGAGG TCATCACGGA GGCTGTACAG AAGTATAATG TTATGTTCGT GTCGAGTGCG 
GGGAATAACG GTCCATGTCT GACCACCGTG GGCTGTCCTG GAGGAACCAC CAGCAGTGTG 
ATCGGAGTGG GAGCGTATGT TACTCCAGAT ATGATGGTGG CCGAGTATTC TCTGAGAGAG 
AAGCTTCCTC CTAACCAGTA CACCTGGTCA TCCCGTGGGC CCTGCACAGA CGGCGCTCTG 
GGCGTCAGTA TTAGTGCTCC GGGCGGCGCC ATCGCCTCCG TGCCCAACTG GACGCTGCGC 
GGCACTCAGC TGATGAACGG CACGTCCATG TCCTCCCCGA ACGCCTGCGG AGGAATCGCC 
CTCGTGCTGT CAGGTCTGAA GCAGAACGGT CTGCGGCCCA CGGTGCCGGC GGTGCGCAGA 
GCTCTGGAGA ACACAGCTCA GAAGGTGGAG GAGATCGAGG TGTTTGCGCA GGGACACGGA 
ATCATACAGG TGGAGAGGGC GTTCGACTAC CTCATGCAGC ACAATTCTCT GGCCTCCAGC 
AGTCTGGGCT TCAGCGTCAG TGCAGGATCT CAGAGAGGAA TCTACCTCAG AGACGCCACA 
CACGTCACTG CACCATCTGA CCACGGCGTC GGCATCGAGC CGATCTTTCC AGAGAACACC 
GAGAACGCAG CTCGTATCTC TCTGCAGCTT CACCTGGCGC TGGTCTGTAA TGCGTCCTGG 
GTTCAGTGTC CGTCCCATCT GGAGCTCATG AATCAGTGCA GGCATGTGAA TGTGCGCATC 
GACCCACAGG GCCTTCGAGA GGGACTGCAC TACACCGAGG TGTGTGGATA CGACACAACT 
GCTCTCAGCG CCGGCCCGCT CTTCAGAGTG CCCATAACCG TTATAATCCC TACCAAAGTA 
TCTGACAGTC GCAGTCAGGA GCTCTCCTTC ACTGATGTTC ACTTCCGGCC AGGACAGATC 
CGTCGCCACT TCATCACCGT CCCTCAAGGA GCATCATGGG CAGAGATCTC TCTGACGTCT 
CACACTGGCG ACGTGTCCTC GAAGTTCGTC CTGCATGCGG TTCATCTGGT GAAGCAGCGA 
GCGTATCGAG CCAACGAGTT CTACAAGTTC TCCTCTCTGC TGGAGAAAGG CTCGCTCACA 
GAGGCCTTTC CAGTGCTGCC AGGTCGGACT CTGGAGCTGT GTATTGCGCG CTGGTGGGCG 
AGTCTCGGTG ATGTCACCAT CGATTATGTC ATTTCCTTCC ACGGCCTGGT GACGTCGCCC 
TCTCCTATTC ACATCCACGC TTCTGAAGGC ATTTCTAGTT TTGAGGTTTG CTCTCCGTTG 
AGATATGAAG ATGTTTCTCC AACAATCACA CTGAAGAACT GGGTTCAGCC GCTCCGGCCT 
GTAAGTTCTA AAATCAAACC GCTGGGCATG AGAGACGTGT TGCCCAATAA CAGGCAGCTC 
TATGAGCTCG TCCTCACCTA TAATTTCCAC CAGCCGAAGA GCGGCGAGGT GACACCCAGC 
TGTCCACTGC TCTGTGAGTT ATTATACGAG TCTGAGTTCG ACAGTCAACT CTGGCTCCTG 
TTTGACCAGA ACAAGAGGCT CATGGGATCT GGAGATGCGT ACCCACACCA GTACTCTCTG 
AAGCTGGAGA AAGGCGACTA CACTGTGCGT CTGCAGGTGC GTCACGAGCA GCAGAGTGAG 
CTGGAGCGTC TGAAGGATCT GCCGTTCGTG GTGTCTCACC GTCTGTCCAA CACTCTCTCG 
CTGGACGTCT ACGAGACGCA TCGCGCCGCC CTGCTGGCCA AGAAGAAGGC CAACTCCATC 
ACACTGAGCC CCGGGGCCTC ACAGCCGTTC TACATCACCA GTCTGCCCGA CGACAAGATC 
CCTAAAGGCT CCGGTCCAGG CGGGTTCATC TCCGGCTCTC TGGTTCTGCC CAAGAGTGAG 
TTCGGCAAAA AAGCAGATAT CGTGCCAATC TTTTACCACC TGGTTCCTGC TCCCAACAAA 
AGCAAGAACG GGAAGGAGAA GGAGAAGGAC CCGGAGAAGG AGAAGGACGT GAAGGAGGAG 
TTCAGTGAGG CGCTGCGGGA CCTGAAGATC CAGTGGATGA GCAAACTGGA CAACAGTTCA 
CTGTATGAGG AGCTGAAGGA GGCGTTTCCT ACACACCTGC CACTGCATGT GCAGAGACTC 
CATCAGCTCG ACTCTGAGAA GGAGCGTCTG AAGCGTCTGC CGGAGATTGT ATCCGCTGCA 
GAGTCAGTGA TGTCACACAT CGATCAGACG GCGCTGGCAG TGTACCTCAC CATGAAGACG 
GACCCACGGC CCGACGCCGC CTCCATCAAG AGTGATATGG AGAAGCAGAA GGCGTCTCTG 
CTGGATGCGC TCTGCAGGAA GGGCTGTGCT CTGGCGGATC AGCTGATGCA GACGCCGCTG 
CAGGACGGGG CCAGTGCCGG ACAGGTGAGC AGCTCGGAGG AGGAGTCAGT GGGCGTGGCC 
AAAGCTCTGA CGGACACCTT CTGGGAGCTG CAGAAGTGGG CGGAGCTTAC AGACTCTAAG 
GTTTTGTCGT TCTCCTATAA GCATGCGCTG GCCAATAAGA TGTACGCCAG AGCCTTGAAA 
TACGCAATAA AGATTGTAGA GGACAAACCC AGCAGAGAGA ACTCCAAGAA CTGCTTACAG 
CTGATGCGTT CTCTGGGCTG GACACACTGC GTCTCGTTCA CTGAGAGCTG GCTGCCGATC 
CTCTTCCCGG CAGACTTCAC ACCCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002660367.2
CDS	96..3845
Translation	MSAHSAEEPFPFHGLLPKKETGAASFLTKYPEYDGRGVLIAILDTGVDPGAPGMQITTDGKPKIVDIIDTTGSGDVNMSTVVEVKEGSVSGLTGRTLKIPAAWVNPSGKYHIGVKNGYDFFPKALKERIQKERKEKLWDPTHRAALAEASRRTEEFDQTHTNPSQMEKLQKEELQCHVELLGMLEKKYSDPGPVYDCISWHDGVTWRAVVDTSECGDLSSCTVLSSYRERQEFCTLGFAEMLNFSVNIYDEGNTLCIVTSGGAHGTHVASIAAGHFPDEPERNGVAPGAQILALKIGDTRLSTMETGTGLIRAMIEVINYKCDLVNYSYGEATHWPNSGRICEVITEAVQKYNVMFVSSAGNNGPCLTTVGCPGGTTSSVIGVGAYVTPDMMVAEYSLREKLPPNQYTWSSRGPCTDGALGVSISAPGGAIASVPNWTLRGTQLMNGTSMSSPNACGGIALVLSGLKQNGLRPTVPAVRRALENTAQKVEEIEVFAQGHGIIQVERAFDYLMQHNSLASSSLGFSVSAGSQRGIYLRDATHVTAPSDHGVGIEPIFPENTENAARISLQLHLALVCNASWVQCPSHLELMNQCRHVNVRIDPQGLREGLHYTEVCGYDTTALSAGPLFRVPITVIIPTKVSDSRSQELSFTDVHFRPGQIRRHFITVPQGASWAEISLTSHTGDVSSKFVLHAVHLVKQRAYRANEFYKFSSLLEKGSLTEAFPVLPGRTLELCIARWWASLGDVTIDYVISFHGLVTSPSPIHIHASEGISSFEVCSPLRYEDVSPTITLKNWVQPLRPVSSKIKPLGMRDVLPNNRQLYELVLTYNFHQPKSGEVTPSCPLLCELLYESEFDSQLWLLFDQNKRLMGSGDAYPHQYSLKLEKGDYTVRLQVRHEQQSELERLKDLPFVVSHRLSNTLSLDVYETHRAALLAKKKANSITLSPGASQPFYITSLPDDKIPKGSGPGGFISGSLVLPKSEFGKKADIVPIFYHLVPAPNKSKNGKEKEKDPEKEKDVKEEFSEALRDLKIQWMSKLDNSSLYEELKEAFPTHLPLHVQRLHQLDSEKERLKRLPEIVSAAESVMSHIDQTALAVYLTMKTDPRPDAASIKSDMEKQKASLLDALCRKGCALADQLMQTPLQDGASAGQVSSSEEESVGVAKALTDTFWELQKWAELTDSKVLSFSYKHALANKMYARALKYAIKIVEDKPSRENSKNCLQLMRSLGWTHCVSFTESWLPILFPADFTPF

Target ORF information:

RefSeq Version	XM_002660321.5
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:ch73-244f7.4 (si:ch73-244f7.4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002660321.5

ORF Insert Sequence:

ATGTCTGCTC ATTCCGCCGA AGAACCGTTC CCGTTTCACG GGCTGCTCCC GAAAAAAGAG 
ACCGGCGCTG CTTCGTTCCT CACTAAGTAC CCGGAGTACG ATGGCCGAGG GGTGCTCATC 
GCGATTCTCG ACACCGGCGT GGACCCAGGG GCCCCGGGCA TGCAGATCAC CACAGACGGA 
AAGCCGAAGA TCGTGGACAT CATCGACACC ACGGGCAGCG GTGACGTGAA CATGAGCACA 
GTGGTGGAGG TGAAGGAGGG CAGTGTGAGC GGTCTGACTG GACGCACGCT TAAAATTCCT 
GCAGCGTGGG TCAATCCGTC TGGGAAATAT CACATTGGAG TGAAAAACGG CTATGATTTC 
TTCCCTAAAG CTCTTAAAGA GAGAATACAG AAAGAGCGCA AGGAGAAGCT GTGGGATCCG 
ACACACCGAG CAGCTCTCGC TGAGGCCAGC CGCAGGACAG AAGAGTTCGA CCAAACACAC 
ACAAACCCCT CACAGATGGA GAAGCTCCAG AAGGAGGAGC TGCAGTGTCA TGTGGAGCTG 
CTGGGAATGC TGGAGAAGAA ATACAGTGAT CCTGGACCCG TGTATGACTG TATATCATGG 
CACGACGGGG TCACGTGGAG GGCTGTGGTG GACACGTCTG AGTGTGGAGA TCTGTCGTCC 
TGCACTGTCC TCAGCTCCTA CAGAGAGAGA CAGGAGTTCT GCACGCTGGG ATTCGCTGAG 
ATGCTCAACT TCTCCGTCAA CATCTATGAT GAGGGAAACA CACTCTGCAT CGTCACCAGC 
GGAGGGGCTC ATGGGACACA CGTGGCCAGC ATCGCAGCAG GTCATTTCCC AGATGAACCC 
GAGCGTAACG GTGTGGCTCC TGGAGCTCAG ATCCTGGCGC TGAAGATCGG AGACACTCGC 
CTGAGCACCA TGGAGACGGG AACCGGCCTC ATACGAGCGA TGATTGAAGT TATAAACTAT 
AAGTGTGATC TTGTGAACTA CAGTTACGGT GAAGCGACGC ATTGGCCAAA CTCAGGGAGA 
ATCTGTGAGG TCATCACGGA GGCTGTACAG AAGTATAATG TTATGTTCGT GTCGAGTGCG 
GGGAATAACG GTCCATGTCT GACCACCGTG GGCTGTCCTG GAGGAACCAC CAGCAGTGTG 
ATCGGAGTGG GAGCGTATGT TACTCCAGAT ATGATGGTGG CCGAGTATTC TCTGAGAGAG 
AAGCTTCCTC CTAACCAGTA CACCTGGTCA TCCCGTGGGC CCTGCACAGA CGGCGCTCTG 
GGCGTCAGTA TTAGTGCTCC GGGCGGCGCC ATCGCCTCCG TGCCCAACTG GACGCTGCGC 
GGCACTCAGC TGATGAACGG CACGTCCATG TCCTCCCCGA ACGCCTGCGG AGGAATCGCC 
CTCGTGCTGT CAGGTCTGAA GCAGAACGGT CTGCGGCCCA CGGTGCCGGC GGTGCGCAGA 
GCTCTGGAGA ACACAGCTCA GAAGGTGGAG GAGATCGAGG TGTTTGCGCA GGGACACGGA 
ATCATACAGG TGGAGAGGGC GTTCGACTAC CTCATGCAGC ACAATTCTCT GGCCTCCAGC 
AGTCTGGGCT TCAGCGTCAG TGCAGGATCT CAGAGAGGAA TCTACCTCAG AGACGCCACA 
CACGTCACTG CACCATCTGA CCACGGCGTC GGCATCGAGC CGATCTTTCC AGAGAACACC 
GAGAACGCAG CTCGTATCTC TCTGCAGCTT CACCTGGCGC TGGTCTGTAA TGCGTCCTGG 
GTTCAGTGTC CGTCCCATCT GGAGCTCATG AATCAGTGCA GGCATGTGAA TGTGCGCATC 
GACCCACAGG GCCTTCGAGA GGGACTGCAC TACACCGAGG TGTGTGGATA CGACACAACT 
GCTCTCAGCG CCGGCCCGCT CTTCAGAGTG CCCATAACCG TTATAATCCC TACCAAAGTA 
TCTGACAGTC GCAGTCAGGA GCTCTCCTTC ACTGATGTTC ACTTCCGGCC AGGACAGATC 
CGTCGCCACT TCATCACCGT CCCTCAAGGA GCATCATGGG CAGAGATCTC TCTGACGTCT 
CACACTGGCG ACGTGTCCTC GAAGTTCGTC CTGCATGCGG TTCATCTGGT GAAGCAGCGA 
GCGTATCGAG CCAACGAGTT CTACAAGTTC TCCTCTCTGC TGGAGAAAGG CTCGCTCACA 
GAGGCCTTTC CAGTGCTGCC AGGTCGGACT CTGGAGCTGT GTATTGCGCG CTGGTGGGCG 
AGTCTCGGTG ATGTCACCAT CGATTATGTC ATTTCCTTCC ACGGCCTGGT GACGTCGCCC 
TCTCCTATTC ACATCCACGC TTCTGAAGGC ATTTCTAGTT TTGAGGTTTG CTCTCCGTTG 
AGATATGAAG ATGTTTCTCC AACAATCACA CTGAAGAACT GGGTTCAGCC GCTCCGGCCT 
GTAAGTTCTA AAATCAAACC GCTGGGCATG AGAGACGTGT TGCCCAATAA CAGGCAGCTC 
TATGAGCTCG TCCTCACCTA TAATTTCCAC CAGCCGAAGA GCGGCGAGGT GACACCCAGC 
TGTCCACTGC TCTGTGAGTT ATTATACGAG TCTGAGTTCG ACAGTCAACT CTGGCTCCTG 
TTTGACCAGA ACAAGAGGCT CATGGGATCT GGAGATGCGT ACCCACACCA GTACTCTCTG 
AAGCTGGAGA AAGGCGACTA CACTGTGCGT CTGCAGGTGC GTCACGAGCA GCAGAGTGAG 
CTGGAGCGTC TGAAGGATCT GCCGTTCGTG GTGTCTCACC GTCTGTCCAA CACTCTCTCG 
CTGGACGTCT ACGAGACGCA TCGCGCCGCC CTGCTGGCCA AGAAGAAGGC CAACTCCATC 
ACACTGAGCC CCGGGGCCTC ACAGCCGTTC TACATCACCA GTCTGCCCGA CGACAAGATC 
CCTAAAGGCT CCGGTCCAGG CGGGTTCATC TCCGGCTCTC TGGTTCTGCC CAAGAGTGAG 
TTCGGCAAAA AAGCAGATAT CGTGCCAATC TTTTACCACC TGGTTCCTGC TCCCAACAAA 
AGCAAGAACG GGAAGGAGAA GGAGAAGGAC CCGGAGAAGG AGAAGGACGT GAAGGAGGAG 
TTCAGTGAGG CGCTGCGGGA CCTGAAGATC CAGTGGATGA GCAAACTGGA CAACAGTTCA 
CTGTATGAGG AGCTGAAGGA GGCGTTTCCT ACACACCTGC CACTGCATGT GCAGAGACTC 
CATCAGCTCG ACTCTGAGAA GGAGCGTCTG AAGCGTCTGC CGGAGATTGT ATCCGCTGCA 
GAGTCAGTGA TGTCACACAT CGATCAGACG GCGCTGGCAG TGTACCTCAC CATGAAGACG 
GACCCACGGC CCGACGCCGC CTCCATCAAG AGTGATATGG AGAAGCAGAA GGCGTCTCTG 
CTGGATGCGC TCTGCAGGAA GGGCTGTGCT CTGGCGGATC AGCTGATGCA GACGCCGCTG 
CAGGACGGGG CCAGTGCCGG ACAGGTGAGC AGCTCGGAGG AGGAGTCAGT GGGCGTGGCC 
AAAGCTCTGA CGGACACCTT CTGGGAGCTG CAGAAGTGGG CGGAGCTTAC AGACTCTAAG 
GTTTTGTCGT TCTCCTATAA GCATGCGCTG GCCAATAAGA TGTACGCCAG AGCCTTGAAA 
TACGCAATAA AGATTGTAGA GGACAAACCC AGCAGAGAGA ACTCCAAGAA CTGCTTACAG 
CTGATGCGTT CTCTGGGCTG GACACACTGC GTCTCGTTCA CTGAGAGCTG GCTGCCGATC 
CTCTTCCCGG CAGACTTCAC ACCCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tpp2 ( XM_002660321.6 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_002660367.2 Danio rerio tripeptidyl peptidase 2 (tpp2), transcript variant X3, mRNA.

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CloneID	ODa05340
Clone ID Related Accession (Same CDS sequence)	XM_002660321.5 , XM_002660321.6
Accession Version	XM_002660321.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3750bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tripeptidyl-peptidase 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_002660321.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCTC ATTCCGCCGA AGAACCGTTC CCGTTTCACG GGCTGCTCCC GAAAAAAGAG 
ACCGGCGCTG CTTCGTTCCT CACTAAGTAC CCGGAGTACG ATGGCCGAGG GGTGCTCATC 
GCGATTCTCG ACACCGGCGT GGACCCAGGG GCCCCGGGCA TGCAGATCAC CACAGACGGA 
AAGCCGAAGA TCGTGGACAT CATCGACACC ACGGGCAGCG GTGACGTGAA CATGAGCACA 
GTGGTGGAGG TGAAGGAGGG CAGTGTGAGC GGTCTGACTG GACGCACGCT TAAAATTCCT 
GCAGCGTGGG TCAATCCGTC TGGGAAATAT CACATTGGAG TGAAAAACGG CTATGATTTC 
TTCCCTAAAG CTCTTAAAGA GAGAATACAG AAAGAGCGCA AGGAGAAGCT GTGGGATCCG 
ACACACCGAG CAGCTCTCGC TGAGGCCAGC CGCAGGACAG AAGAGTTCGA CCAAACACAC 
ACAAACCCCT CACAGATGGA GAAGCTCCAG AAGGAGGAGC TGCAGTGTCA TGTGGAGCTG 
CTGGGAATGC TGGAGAAGAA ATACAGTGAT CCTGGACCCG TGTATGACTG TATATCATGG 
CACGACGGGG TCACGTGGAG GGCTGTGGTG GACACGTCTG AGTGTGGAGA TCTGTCGTCC 
TGCACTGTCC TCAGCTCCTA CAGAGAGAGA CAGGAGTTCT GCACGCTGGG ATTCGCTGAG 
ATGCTCAACT TCTCCGTCAA CATCTATGAT GAGGGAAACA CACTCTGCAT CGTCACCAGC 
GGAGGGGCTC ATGGGACACA CGTGGCCAGC ATCGCAGCAG GTCATTTCCC AGATGAACCC 
GAGCGTAACG GTGTGGCTCC TGGAGCTCAG ATCCTGGCGC TGAAGATCGG AGACACTCGC 
CTGAGCACCA TGGAGACGGG AACCGGCCTC ATACGAGCGA TGATTGAAGT TATAAACTAT 
AAGTGTGATC TTGTGAACTA CAGTTACGGT GAAGCGACGC ATTGGCCAAA CTCAGGGAGA 
ATCTGTGAGG TCATCACGGA GGCTGTACAG AAGTATAATG TTATGTTCGT GTCGAGTGCG 
GGGAATAACG GTCCATGTCT GACCACCGTG GGCTGTCCTG GAGGAACCAC CAGCAGTGTG 
ATCGGAGTGG GAGCGTATGT TACTCCAGAT ATGATGGTGG CCGAGTATTC TCTGAGAGAG 
AAGCTTCCTC CTAACCAGTA CACCTGGTCA TCCCGTGGGC CCTGCACAGA CGGCGCTCTG 
GGCGTCAGTA TTAGTGCTCC GGGCGGCGCC ATCGCCTCCG TGCCCAACTG GACGCTGCGC 
GGCACTCAGC TGATGAACGG CACGTCCATG TCCTCCCCGA ACGCCTGCGG AGGAATCGCC 
CTCGTGCTGT CAGGTCTGAA GCAGAACGGT CTGCGGCCCA CGGTGCCGGC GGTGCGCAGA 
GCTCTGGAGA ACACAGCTCA GAAGGTGGAG GAGATCGAGG TGTTTGCGCA GGGACACGGA 
ATCATACAGG TGGAGAGGGC GTTCGACTAC CTCATGCAGC ACAATTCTCT GGCCTCCAGC 
AGTCTGGGCT TCAGCGTCAG TGCAGGATCT CAGAGAGGAA TCTACCTCAG AGACGCCACA 
CACGTCACTG CACCATCTGA CCACGGCGTC GGCATCGAGC CGATCTTTCC AGAGAACACC 
GAGAACGCAG CTCGTATCTC TCTGCAGCTT CACCTGGCGC TGGTCTGTAA TGCGTCCTGG 
GTTCAGTGTC CGTCCCATCT GGAGCTCATG AATCAGTGCA GGCATGTGAA TGTGCGCATC 
GACCCACAGG GCCTTCGAGA GGGACTGCAC TACACCGAGG TGTGTGGATA CGACACAACT 
GCTCTCAGCG CCGGCCCGCT CTTCAGAGTG CCCATAACCG TTATAATCCC TACCAAAGTA 
TCTGACAGTC GCAGTCAGGA GCTCTCCTTC ACTGATGTTC ACTTCCGGCC AGGACAGATC 
CGTCGCCACT TCATCACCGT CCCTCAAGGA GCATCATGGG CAGAGATCTC TCTGACGTCT 
CACACTGGCG ACGTGTCCTC GAAGTTCGTC CTGCATGCGG TTCATCTGGT GAAGCAGCGA 
GCGTATCGAG CCAACGAGTT CTACAAGTTC TCCTCTCTGC TGGAGAAAGG CTCGCTCACA 
GAGGCCTTTC CAGTGCTGCC AGGTCGGACT CTGGAGCTGT GTATTGCGCG CTGGTGGGCG 
AGTCTCGGTG ATGTCACCAT CGATTATGTC ATTTCCTTCC ACGGCCTGGT GACGTCGCCC 
TCTCCTATTC ACATCCACGC TTCTGAAGGC ATTTCTAGTT TTGAGGTTTG CTCTCCGTTG 
AGATATGAAG ATGTTTCTCC AACAATCACA CTGAAGAACT GGGTTCAGCC GCTCCGGCCT 
GTAAGTTCTA AAATCAAACC GCTGGGCATG AGAGACGTGT TGCCCAATAA CAGGCAGCTC 
TATGAGCTCG TCCTCACCTA TAATTTCCAC CAGCCGAAGA GCGGCGAGGT GACACCCAGC 
TGTCCACTGC TCTGTGAGTT ATTATACGAG TCTGAGTTCG ACAGTCAACT CTGGCTCCTG 
TTTGACCAGA ACAAGAGGCT CATGGGATCT GGAGATGCGT ACCCACACCA GTACTCTCTG 
AAGCTGGAGA AAGGCGACTA CACTGTGCGT CTGCAGGTGC GTCACGAGCA GCAGAGTGAG 
CTGGAGCGTC TGAAGGATCT GCCGTTCGTG GTGTCTCACC GTCTGTCCAA CACTCTCTCG 
CTGGACGTCT ACGAGACGCA TCGCGCCGCC CTGCTGGCCA AGAAGAAGGC CAACTCCATC 
ACACTGAGCC CCGGGGCCTC ACAGCCGTTC TACATCACCA GTCTGCCCGA CGACAAGATC 
CCTAAAGGCT CCGGTCCAGG CGGGTTCATC TCCGGCTCTC TGGTTCTGCC CAAGAGTGAG 
TTCGGCAAAA AAGCAGATAT CGTGCCAATC TTTTACCACC TGGTTCCTGC TCCCAACAAA 
AGCAAGAACG GGAAGGAGAA GGAGAAGGAC CCGGAGAAGG AGAAGGACGT GAAGGAGGAG 
TTCAGTGAGG CGCTGCGGGA CCTGAAGATC CAGTGGATGA GCAAACTGGA CAACAGTTCA 
CTGTATGAGG AGCTGAAGGA GGCGTTTCCT ACACACCTGC CACTGCATGT GCAGAGACTC 
CATCAGCTCG ACTCTGAGAA GGAGCGTCTG AAGCGTCTGC CGGAGATTGT ATCCGCTGCA 
GAGTCAGTGA TGTCACACAT CGATCAGACG GCGCTGGCAG TGTACCTCAC CATGAAGACG 
GACCCACGGC CCGACGCCGC CTCCATCAAG AGTGATATGG AGAAGCAGAA GGCGTCTCTG 
CTGGATGCGC TCTGCAGGAA GGGCTGTGCT CTGGCGGATC AGCTGATGCA GACGCCGCTG 
CAGGACGGGG CCAGTGCCGG ACAGGTGAGC AGCTCGGAGG AGGAGTCAGT GGGCGTGGCC 
AAAGCTCTGA CGGACACCTT CTGGGAGCTG CAGAAGTGGG CGGAGCTTAC AGACTCTAAG 
GTTTTGTCGT TCTCCTATAA GCATGCGCTG GCCAATAAGA TGTACGCCAG AGCCTTGAAA 
TACGCAATAA AGATTGTAGA GGACAAACCC AGCAGAGAGA ACTCCAAGAA CTGCTTACAG 
CTGATGCGTT CTCTGGGCTG GACACACTGC GTCTCGTTCA CTGAGAGCTG GCTGCCGATC 
CTCTTCCCGG CAGACTTCAC ACCCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002660367.2
CDS	92..3841
Translation	MSAHSAEEPFPFHGLLPKKETGAASFLTKYPEYDGRGVLIAILDTGVDPGAPGMQITTDGKPKIVDIIDTTGSGDVNMSTVVEVKEGSVSGLTGRTLKIPAAWVNPSGKYHIGVKNGYDFFPKALKERIQKERKEKLWDPTHRAALAEASRRTEEFDQTHTNPSQMEKLQKEELQCHVELLGMLEKKYSDPGPVYDCISWHDGVTWRAVVDTSECGDLSSCTVLSSYRERQEFCTLGFAEMLNFSVNIYDEGNTLCIVTSGGAHGTHVASIAAGHFPDEPERNGVAPGAQILALKIGDTRLSTMETGTGLIRAMIEVINYKCDLVNYSYGEATHWPNSGRICEVITEAVQKYNVMFVSSAGNNGPCLTTVGCPGGTTSSVIGVGAYVTPDMMVAEYSLREKLPPNQYTWSSRGPCTDGALGVSISAPGGAIASVPNWTLRGTQLMNGTSMSSPNACGGIALVLSGLKQNGLRPTVPAVRRALENTAQKVEEIEVFAQGHGIIQVERAFDYLMQHNSLASSSLGFSVSAGSQRGIYLRDATHVTAPSDHGVGIEPIFPENTENAARISLQLHLALVCNASWVQCPSHLELMNQCRHVNVRIDPQGLREGLHYTEVCGYDTTALSAGPLFRVPITVIIPTKVSDSRSQELSFTDVHFRPGQIRRHFITVPQGASWAEISLTSHTGDVSSKFVLHAVHLVKQRAYRANEFYKFSSLLEKGSLTEAFPVLPGRTLELCIARWWASLGDVTIDYVISFHGLVTSPSPIHIHASEGISSFEVCSPLRYEDVSPTITLKNWVQPLRPVSSKIKPLGMRDVLPNNRQLYELVLTYNFHQPKSGEVTPSCPLLCELLYESEFDSQLWLLFDQNKRLMGSGDAYPHQYSLKLEKGDYTVRLQVRHEQQSELERLKDLPFVVSHRLSNTLSLDVYETHRAALLAKKKANSITLSPGASQPFYITSLPDDKIPKGSGPGGFISGSLVLPKSEFGKKADIVPIFYHLVPAPNKSKNGKEKEKDPEKEKDVKEEFSEALRDLKIQWMSKLDNSSLYEELKEAFPTHLPLHVQRLHQLDSEKERLKRLPEIVSAAESVMSHIDQTALAVYLTMKTDPRPDAASIKSDMEKQKASLLDALCRKGCALADQLMQTPLQDGASAGQVSSSEEESVGVAKALTDTFWELQKWAELTDSKVLSFSYKHALANKMYARALKYAIKIVEDKPSRENSKNCLQLMRSLGWTHCVSFTESWLPILFPADFTPF

Target ORF information:

RefSeq Version	XM_002660321.6
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tripeptidyl peptidase 2 (tpp2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002660321.6

ORF Insert Sequence:

ATGTCTGCTC ATTCCGCCGA AGAACCGTTC CCGTTTCACG GGCTGCTCCC GAAAAAAGAG 
ACCGGCGCTG CTTCGTTCCT CACTAAGTAC CCGGAGTACG ATGGCCGAGG GGTGCTCATC 
GCGATTCTCG ACACCGGCGT GGACCCAGGG GCCCCGGGCA TGCAGATCAC CACAGACGGA 
AAGCCGAAGA TCGTGGACAT CATCGACACC ACGGGCAGCG GTGACGTGAA CATGAGCACA 
GTGGTGGAGG TGAAGGAGGG CAGTGTGAGC GGTCTGACTG GACGCACGCT TAAAATTCCT 
GCAGCGTGGG TCAATCCGTC TGGGAAATAT CACATTGGAG TGAAAAACGG CTATGATTTC 
TTCCCTAAAG CTCTTAAAGA GAGAATACAG AAAGAGCGCA AGGAGAAGCT GTGGGATCCG 
ACACACCGAG CAGCTCTCGC TGAGGCCAGC CGCAGGACAG AAGAGTTCGA CCAAACACAC 
ACAAACCCCT CACAGATGGA GAAGCTCCAG AAGGAGGAGC TGCAGTGTCA TGTGGAGCTG 
CTGGGAATGC TGGAGAAGAA ATACAGTGAT CCTGGACCCG TGTATGACTG TATATCATGG 
CACGACGGGG TCACGTGGAG GGCTGTGGTG GACACGTCTG AGTGTGGAGA TCTGTCGTCC 
TGCACTGTCC TCAGCTCCTA CAGAGAGAGA CAGGAGTTCT GCACGCTGGG ATTCGCTGAG 
ATGCTCAACT TCTCCGTCAA CATCTATGAT GAGGGAAACA CACTCTGCAT CGTCACCAGC 
GGAGGGGCTC ATGGGACACA CGTGGCCAGC ATCGCAGCAG GTCATTTCCC AGATGAACCC 
GAGCGTAACG GTGTGGCTCC TGGAGCTCAG ATCCTGGCGC TGAAGATCGG AGACACTCGC 
CTGAGCACCA TGGAGACGGG AACCGGCCTC ATACGAGCGA TGATTGAAGT TATAAACTAT 
AAGTGTGATC TTGTGAACTA CAGTTACGGT GAAGCGACGC ATTGGCCAAA CTCAGGGAGA 
ATCTGTGAGG TCATCACGGA GGCTGTACAG AAGTATAATG TTATGTTCGT GTCGAGTGCG 
GGGAATAACG GTCCATGTCT GACCACCGTG GGCTGTCCTG GAGGAACCAC CAGCAGTGTG 
ATCGGAGTGG GAGCGTATGT TACTCCAGAT ATGATGGTGG CCGAGTATTC TCTGAGAGAG 
AAGCTTCCTC CTAACCAGTA CACCTGGTCA TCCCGTGGGC CCTGCACAGA CGGCGCTCTG 
GGCGTCAGTA TTAGTGCTCC GGGCGGCGCC ATCGCCTCCG TGCCCAACTG GACGCTGCGC 
GGCACTCAGC TGATGAACGG CACGTCCATG TCCTCCCCGA ACGCCTGCGG AGGAATCGCC 
CTCGTGCTGT CAGGTCTGAA GCAGAACGGT CTGCGGCCCA CGGTGCCGGC GGTGCGCAGA 
GCTCTGGAGA ACACAGCTCA GAAGGTGGAG GAGATCGAGG TGTTTGCGCA GGGACACGGA 
ATCATACAGG TGGAGAGGGC GTTCGACTAC CTCATGCAGC ACAATTCTCT GGCCTCCAGC 
AGTCTGGGCT TCAGCGTCAG TGCAGGATCT CAGAGAGGAA TCTACCTCAG AGACGCCACA 
CACGTCACTG CACCATCTGA CCACGGCGTC GGCATCGAGC CGATCTTTCC AGAGAACACC 
GAGAACGCAG CTCGTATCTC TCTGCAGCTT CACCTGGCGC TGGTCTGTAA TGCGTCCTGG 
GTTCAGTGTC CGTCCCATCT GGAGCTCATG AATCAGTGCA GGCATGTGAA TGTGCGCATC 
GACCCACAGG GCCTTCGAGA GGGACTGCAC TACACCGAGG TGTGTGGATA CGACACAACT 
GCTCTCAGCG CCGGCCCGCT CTTCAGAGTG CCCATAACCG TTATAATCCC TACCAAAGTA 
TCTGACAGTC GCAGTCAGGA GCTCTCCTTC ACTGATGTTC ACTTCCGGCC AGGACAGATC 
CGTCGCCACT TCATCACCGT CCCTCAAGGA GCATCATGGG CAGAGATCTC TCTGACGTCT 
CACACTGGCG ACGTGTCCTC GAAGTTCGTC CTGCATGCGG TTCATCTGGT GAAGCAGCGA 
GCGTATCGAG CCAACGAGTT CTACAAGTTC TCCTCTCTGC TGGAGAAAGG CTCGCTCACA 
GAGGCCTTTC CAGTGCTGCC AGGTCGGACT CTGGAGCTGT GTATTGCGCG CTGGTGGGCG 
AGTCTCGGTG ATGTCACCAT CGATTATGTC ATTTCCTTCC ACGGCCTGGT GACGTCGCCC 
TCTCCTATTC ACATCCACGC TTCTGAAGGC ATTTCTAGTT TTGAGGTTTG CTCTCCGTTG 
AGATATGAAG ATGTTTCTCC AACAATCACA CTGAAGAACT GGGTTCAGCC GCTCCGGCCT 
GTAAGTTCTA AAATCAAACC GCTGGGCATG AGAGACGTGT TGCCCAATAA CAGGCAGCTC 
TATGAGCTCG TCCTCACCTA TAATTTCCAC CAGCCGAAGA GCGGCGAGGT GACACCCAGC 
TGTCCACTGC TCTGTGAGTT ATTATACGAG TCTGAGTTCG ACAGTCAACT CTGGCTCCTG 
TTTGACCAGA ACAAGAGGCT CATGGGATCT GGAGATGCGT ACCCACACCA GTACTCTCTG 
AAGCTGGAGA AAGGCGACTA CACTGTGCGT CTGCAGGTGC GTCACGAGCA GCAGAGTGAG 
CTGGAGCGTC TGAAGGATCT GCCGTTCGTG GTGTCTCACC GTCTGTCCAA CACTCTCTCG 
CTGGACGTCT ACGAGACGCA TCGCGCCGCC CTGCTGGCCA AGAAGAAGGC CAACTCCATC 
ACACTGAGCC CCGGGGCCTC ACAGCCGTTC TACATCACCA GTCTGCCCGA CGACAAGATC 
CCTAAAGGCT CCGGTCCAGG CGGGTTCATC TCCGGCTCTC TGGTTCTGCC CAAGAGTGAG 
TTCGGCAAAA AAGCAGATAT CGTGCCAATC TTTTACCACC TGGTTCCTGC TCCCAACAAA 
AGCAAGAACG GGAAGGAGAA GGAGAAGGAC CCGGAGAAGG AGAAGGACGT GAAGGAGGAG 
TTCAGTGAGG CGCTGCGGGA CCTGAAGATC CAGTGGATGA GCAAACTGGA CAACAGTTCA 
CTGTATGAGG AGCTGAAGGA GGCGTTTCCT ACACACCTGC CACTGCATGT GCAGAGACTC 
CATCAGCTCG ACTCTGAGAA GGAGCGTCTG AAGCGTCTGC CGGAGATTGT ATCCGCTGCA 
GAGTCAGTGA TGTCACACAT CGATCAGACG GCGCTGGCAG TGTACCTCAC CATGAAGACG 
GACCCACGGC CCGACGCCGC CTCCATCAAG AGTGATATGG AGAAGCAGAA GGCGTCTCTG 
CTGGATGCGC TCTGCAGGAA GGGCTGTGCT CTGGCGGATC AGCTGATGCA GACGCCGCTG 
CAGGACGGGG CCAGTGCCGG ACAGGTGAGC AGCTCGGAGG AGGAGTCAGT GGGCGTGGCC 
AAAGCTCTGA CGGACACCTT CTGGGAGCTG CAGAAGTGGG CGGAGCTTAC AGACTCTAAG 
GTTTTGTCGT TCTCCTATAA GCATGCGCTG GCCAATAAGA TGTACGCCAG AGCCTTGAAA 
TACGCAATAA AGATTGTAGA GGACAAACCC AGCAGAGAGA ACTCCAAGAA CTGCTTACAG 
CTGATGCGTT CTCTGGGCTG GACACACTGC GTCTCGTTCA CTGAGAGCTG GCTGCCGATC 
CTCTTCCCGG CAGACTTCAC ACCCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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tpp2 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Eメール

電話

FAX

オンラインフォーム

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