Adamts4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Adamts4
Entrez Gene ID	66015
Full Name	ADAM metallopeptidase with thrombospondin type 1 motif, 4
General protein information	Preferred Names ADAM metallopeptidase with thrombospondin type 1 motif, 4 Names A disintegrin and metalloproteinase with thrombospondin motifs 4 ADAM-TS 4 ADAM-TS4 ADAMTS-4 Aggrecanase a disintegrin and metallopeptidase with thrombospondin motifs 4 a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 4 aggrecanase-1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 13 Map Location: 13q24
Summary	a disintegrin and metalloproteinase; may play a role in the progression of Alzheimers disease [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_023959.1	NP_076449.1	A disintegrin and metalloproteinase with thrombospondin motifs 4

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ADAMTS4	XP_513940.2
Canis lupus familiaris (dog)	ADAMTS4	XP_545768.3
Xenopus tropicalis (tropical clawed frog)	adamts4	XP_004919795.1
Rattus norvegicus (Norway rat)	Adamts4	NP_076449.1
Homo sapiens (human)	ADAMTS4	NP_005090.3
	ADAMTS4	XP_002808295.1
Bos taurus (cattle)	ADAMTS4	NP_858053.1
Mus musculus (house mouse)	Adamts4	NP_766433.1

Gene Ontology
Bibliography

Related articles in PubMed

Comparative proteomic analysis of supportive and unsupportive extracellular matrix substrates for human embryonic stem cell maintenance.
Soteriou D, Iskender B, Byron A, Humphries JD, Borg-Bartolo S, Haddock MC, Baxter MA, Knight D, Humphries MJ, Kimber SJ
The Journal of biological chemistry288(26)18716-31(2013 Jun)

Cochlin produced by follicular dendritic cells promotes antibacterial innate immunity.
Py BF, Gonzalez SF, Long K, Kim MS, Kim YA, Zhu H, Yao J, Degauque N, Villet R, Ymele-Leki P, Gadjeva M, Pier GB, Carroll MC, Yuan J
Immunity38(5)1063-72(2013 May)

Rat tail static compression model mimics extracellular matrix metabolic imbalances of matrix metalloproteinases, aggrecanases, and tissue inhibitors of metalloproteinases in intervertebral disc degeneration.
Yurube T, Takada T, Suzuki T, Kakutani K, Maeno K, Doita M, Kurosaka M, Nishida K
Arthritis research & therapy14(2)R51(2012 Mar)

Targeting bone alleviates osteoarthritis in osteopenic mice and modulates cartilage catabolism.
Funck-Brentano T, Lin H, Hay E, Ah Kioon MD, Schiltz C, Hannouche D, Nizard R, Liot? F, Orcel P, de Vernejoul MC, Cohen-Solal ME
PloS one7(3)e33543(2012)

The quantification of ADAMTS4 and 8 expression and selection of reference genes for quantitative real-time PCR analysis in myocardial infarction.
Zhao C, Zha Y, Wu X, Chen L, Shi J, Cui L
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie65(8)555-9(2011 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

ADAMTS4 mRNA increased at 3 h after myocardial infarction, peaked at 6 h, then decreased rapidly; protein levels of ADAMTS4 were significantly increased at 6 h, 12 h, 24 h and 3 d after MI.
Title: The quantification of ADAMTS4 and 8 expression and selection of reference genes for quantitative real-time PCR analysis in myocardial infarction.

Together these data demonstrate that inhibition of endogenous aggrecanases facilitates chondrocyte differentiation and chondrocyte-engineered cartilage formation in vitro.
Title: Lentivirus-mediated knockdown of aggrecanase-1 and -2 promotes chondrocyte-engineered cartilage formation in vitro.

Inhibition of aggrecanase-1 decreases aggrecan degradation in cultured rat chondrocytes.
Title: [Effects of RNA interference against aggrecanase 1 gene on extracellular matrix metabolism of cultured chondrocytes in vitro].

ADAMTS-5 mainly contributed to ECM metabolism in growth plate and condylar cartilage during growth. ADAMTS-1 and ADAMTS-4 may be involved in ECM turn over in articular cartilage.
Title: Comparison of age-dependent expression of aggrecan and ADAMTSs in mandibular condylar cartilage, tibial growth plate, and articular cartilage in rats.

This study suggests that dental pulp cells, odontoblasts, cementoblasts, cementocytes, periodontal ligament cells, osteoblasts and osteocytes may be involved in both production and degradation of versican with secreting ADAMTS1, ADAMTS4 and ADAMTS5.
Title: Expression of versican and ADAMTS during rat tooth eruption.

The following Adamts4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Adamts4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa06644	NM_023959.1 Latest version!	Rattus norvegicus ADAM metallopeptidase with thrombospondin type 1 motif, 4 (Adamts4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Adamts4 ( NM_023959.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_076449.1 Rattus norvegicus ADAM metallopeptidase with thrombospondin type 1 motif, 4 (Adamts4), mRNA.

Price & Availability↑

CloneID	ORa06644
Clone ID Related Accession (Same CDS sequence)	NM_023959.1
Accession Version	NM_023959.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2556bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Rattus norvegicus(Norway rat)
Product	A disintegrin and metalloproteinase with thrombospondin motifs 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC099236.8, CB614460.1, AB042271.1, AB042272.1 and AB042273.1. On or before Dec 17, 2009 this sequence version replaced XM_341154.3, XM_001053685.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCTCAA TCCATCCCAG CTGCAGCACT GGTACCATGT CCAGGATGGG CTTGCATCCC 
AGGAGGGGCT TGACTGGGCA CAGACTGCGA AGACTCCAAC CCTGCCTGCC GCCCCGCACT 
GTGCCTTTCT CTGGTTTGGA GCGGTGGCAG CAGCTGCTGC TGCTGGCCTC CCTCCTGCCT 
TTAGCCTGGT CAGCCAGCCC CCTCCCCCGA GAGGAGGAGA TCGTGTTTCC AGAGAAGCTC 
AACGGCAGTA GTATCATACC TGGCTCAGGC GTCCCTGCCA GGCTGCTGTA CCGATTACCA 
GCCTTTGGGG AGATATTGTT ACTAGAACTA GAGCAGGATC CTGGGGTGCA GGTAGAGGGG 
CTGACAGTAC AGTACCTGGG CCAGGCACCT GAGATGCTGG GTGGGGCAGA GCCAGGTACC 
TACCTGACTG GCACCATCAA TGGAGATCCG GAGTCGGTGG CGTCTCTGCA CTGGGACAGG 
GGAGCCCTAT TGGGGGTATT GCAGTACCGG GGGGCCGAAC TCCACCTCCA GCCTCTGGAA 
GGAGGCGCCC TTAACTCTGC TGGGGGACCA GGGGCTCACA TCCTACGCCG GAAGAGTCCT 
GCCAGCAACC GAGGTCCCAT ATGCAACGTC AAGGCTCCTT CTGGGAGCCC CAGTCCCATT 
TCCCGCAGAA ACAAGCGCTT CGCTTCGCTG AGTAGATTCG TGGAGACACT GGTGGTGGCA 
GATGACAAGA TGGCAGCATT CCACGGGGCA GGGTTAAAGC ACTACCTGCT GACGGTCATG 
GCCGCCGCTG CCAAAGCCTT TAAGCATCCA AGCATCCGAA ACCCTGTCAA CTTGGTGGTG 
ACTCGTCTAG TAATCCTGGG GTCGGGCCAG GAAGTGCCCC AAGTGGGGCC AAGTGCCGCC 
CAGACCCTAC GCAGCTTCTG CACCTGGCAG AAAGGCCTAA ACCCTCCGAA CGACTCAGAT 
CCTGACCACT TTGACACGGC CATTCTGTTC ACCCGGCAGG ACCTGTGTGG GGTCTCCACC 
TGTGACGCTC TGGGTATGGC TGGTGTGGGC ACAGTGTGTG ACCCAGCTAG GAGCTGTGCT 
ATTGTGGAGG ACGATGGGCT CCAGTCGGCC TTCACCGCCG CTCATGAACT GGGCCATGTC 
TTCAACATGC TCCATGATAA CTCCAAACCG TGTGCTAATT TGAATGGGCA GGGGAGTTCC 
TCTCGCCATG TCATGGCTCC TGTCATGGCT CACGTGGACC CTGAAGAGCC CTGGTCCCCC 
TGCAGTGCCC GATTCATCAC TGACTTCCTG GACAATGGCT ATGGACACTG CCTCTTAGAC 
AAACCAGAGG CTCCCCTGCA TCTGCCAGTG ACTTTTCCTG GCAAGGACTA TGATGCTGAC 
CGCCAGTGCC AACTGACCTT TGGTCCTGAC TCAAGCCATT GTCCACAGCT GCCACCGCCC 
TGTGCTGCCC TCTGGTGCTT TGGCCACCTC AATGGCCATG CCATGTGTCA GACTAAGCAC 
TCACCCTGGG CCGATGGTAC TCCCTGCGGA CCTGCACAGG CCTGTATGGG TGGCCGCTGT 
CTCCACGTGG ACCAGCTCAA GGACTTCAAT ATTCCTCAGG CTGGAGGCTG GGGTCCCTGG 
GGACCATGGG GTGACTGCTC CAGGACTTGT GGAGGTGGTG TCCAATTCTC CTCCCGGGAC 
TGCACAAAGC CTGTCCCCCG GAATGGTGGA AAGTATTGTG AGGGCCGCCG TACCCCGTTC 
CGCTCCTGTA ACACTAAGAA CTGCCCACAT GGCTCAGCAT TGACCTTCCG TGAAGAGCAG 
TGTGCTGCCT ACAACCACCG AACCGACCTC TTCAAGAGTT TTCCGGGGCC CATGGACTGG 
GTTCCGCGCT ACACAGGTGT AGCCCCTCGA GACCAGTGCA AACTCACCTG CCAGGCCCGG 
GCACTGGGCT ACTACTATGT ACTGGAGCCA CGGGTGGCAG ATGGAACTCC CTGTTCCCCA 
GACTCTTCCT CTGTCTGTGT CCAGGGCCGC TGTATCCACG CTGGCTGTGA TCGAATCATT 
GGCTCCAAAA AGAAATTTGA CAAGTGCATG GTGTGCGGTG GGAATGGCTC TAGCTGCAGC 
AAGCAGTCAG GCTCCTTCAA AAAATTCAGG TACGGATACA GCGATGTGGT CACGATCCCT 
GCGGGACGCA CCCATATCCT TGTACGGCAG CAGGGAGGAT CTGGCCTCAA GAGCATCTAC 
CTGGCCCTGA AGCTAGCAGA TGGTTCTTAC GCCCTCAATG GTGAATACAC GCTGATGCCC 
TCCTCAACAG ATGTGGTACT TCCTGGGGCA GTCAGCTTGC GCTATAGCGG ACGCACGGCA 
GCCTCAGAGA CACTGTCTGG ACATGGGCCG CTGGCCCAGC CCTTGACGCT GCAAGTTCTG 
GTGGCTGGCA ACCCACAGAA TGTACGTCTG CGGTACAGTT TCTTTGTCCC GCGGCCAGTC 
CCTTCAACGC CACGCCCTCC TCCCCAAAAC TGGCTGCAAC GCAGGGCAGA GATTCTGGAG 
ATCCTTCGGA AGCGAACCTG GGCAGGCCGG AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_076449.1
CDS	1..2556
Translation	MASIHPSCSTGTMSRMGLHPRRGLTGHRLRRLQPCLPPRTVPFSGLERWQQLLLLASLLPLAWSASPLPREEEIVFPEKLNGSSIIPGSGVPARLLYRLPAFGEILLLELEQDPGVQVEGLTVQYLGQAPEMLGGAEPGTYLTGTINGDPESVASLHWDRGALLGVLQYRGAELHLQPLEGGALNSAGGPGAHILRRKSPASNRGPICNVKAPSGSPSPISRRNKRFASLSRFVETLVVADDKMAAFHGAGLKHYLLTVMAAAAKAFKHPSIRNPVNLVVTRLVILGSGQEVPQVGPSAAQTLRSFCTWQKGLNPPNDSDPDHFDTAILFTRQDLCGVSTCDALGMAGVGTVCDPARSCAIVEDDGLQSAFTAAHELGHVFNMLHDNSKPCANLNGQGSSSRHVMAPVMAHVDPEEPWSPCSARFITDFLDNGYGHCLLDKPEAPLHLPVTFPGKDYDADRQCQLTFGPDSSHCPQLPPPCAALWCFGHLNGHAMCQTKHSPWADGTPCGPAQACMGGRCLHVDQLKDFNIPQAGGWGPWGPWGDCSRTCGGGVQFSSRDCTKPVPRNGGKYCEGRRTPFRSCNTKNCPHGSALTFREEQCAAYNHRTDLFKSFPGPMDWVPRYTGVAPRDQCKLTCQARALGYYYVLEPRVADGTPCSPDSSSVCVQGRCIHAGCDRIIGSKKKFDKCMVCGGNGSSCSKQSGSFKKFRYGYSDVVTIPAGRTHILVRQQGGSGLKSIYLALKLADGSYALNGEYTLMPSSTDVVLPGAVSLRYSGRTAASETLSGHGPLAQPLTLQVLVAGNPQNVRLRYSFFVPRPVPSTPRPPPQNWLQRRAEILEILRKRTWAGRK

Target ORF information:

RefSeq Version	NM_023959.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ADAM metallopeptidase with thrombospondin type 1 motif, 4 (Adamts4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_023959.1

ORF Insert Sequence:

ATGGCCTCAA TCCATCCCAG CTGCAGCACT GGTACCATGT CCAGGATGGG CTTGCATCCC 
AGGAGGGGCT TGACTGGGCA CAGACTGCGA AGACTCCAAC CCTGCCTGCC GCCCCGCACT 
GTGCCTTTCT CTGGTTTGGA GCGGTGGCAG CAGCTGCTGC TGCTGGCCTC CCTCCTGCCT 
TTAGCCTGGT CAGCCAGCCC CCTCCCCCGA GAGGAGGAGA TCGTGTTTCC AGAGAAGCTC 
AACGGCAGTA GTATCATACC TGGCTCAGGC GTCCCTGCCA GGCTGCTGTA CCGATTACCA 
GCCTTTGGGG AGATATTGTT ACTAGAACTA GAGCAGGATC CTGGGGTGCA GGTAGAGGGG 
CTGACAGTAC AGTACCTGGG CCAGGCACCT GAGATGCTGG GTGGGGCAGA GCCAGGTACC 
TACCTGACTG GCACCATCAA TGGAGATCCG GAGTCGGTGG CGTCTCTGCA CTGGGACAGG 
GGAGCCCTAT TGGGGGTATT GCAGTACCGG GGGGCCGAAC TCCACCTCCA GCCTCTGGAA 
GGAGGCGCCC TTAACTCTGC TGGGGGACCA GGGGCTCACA TCCTACGCCG GAAGAGTCCT 
GCCAGCAACC GAGGTCCCAT ATGCAACGTC AAGGCTCCTT CTGGGAGCCC CAGTCCCATT 
TCCCGCAGAA ACAAGCGCTT CGCTTCGCTG AGTAGATTCG TGGAGACACT GGTGGTGGCA 
GATGACAAGA TGGCAGCATT CCACGGGGCA GGGTTAAAGC ACTACCTGCT GACGGTCATG 
GCCGCCGCTG CCAAAGCCTT TAAGCATCCA AGCATCCGAA ACCCTGTCAA CTTGGTGGTG 
ACTCGTCTAG TAATCCTGGG GTCGGGCCAG GAAGTGCCCC AAGTGGGGCC AAGTGCCGCC 
CAGACCCTAC GCAGCTTCTG CACCTGGCAG AAAGGCCTAA ACCCTCCGAA CGACTCAGAT 
CCTGACCACT TTGACACGGC CATTCTGTTC ACCCGGCAGG ACCTGTGTGG GGTCTCCACC 
TGTGACGCTC TGGGTATGGC TGGTGTGGGC ACAGTGTGTG ACCCAGCTAG GAGCTGTGCT 
ATTGTGGAGG ACGATGGGCT CCAGTCGGCC TTCACCGCCG CTCATGAACT GGGCCATGTC 
TTCAACATGC TCCATGATAA CTCCAAACCG TGTGCTAATT TGAATGGGCA GGGGAGTTCC 
TCTCGCCATG TCATGGCTCC TGTCATGGCT CACGTGGACC CTGAAGAGCC CTGGTCCCCC 
TGCAGTGCCC GATTCATCAC TGACTTCCTG GACAATGGCT ATGGACACTG CCTCTTAGAC 
AAACCAGAGG CTCCCCTGCA TCTGCCAGTG ACTTTTCCTG GCAAGGACTA TGATGCTGAC 
CGCCAGTGCC AACTGACCTT TGGTCCTGAC TCAAGCCATT GTCCACAGCT GCCACCGCCC 
TGTGCTGCCC TCTGGTGCTT TGGCCACCTC AATGGCCATG CCATGTGTCA GACTAAGCAC 
TCACCCTGGG CCGATGGTAC TCCCTGCGGA CCTGCACAGG CCTGTATGGG TGGCCGCTGT 
CTCCACGTGG ACCAGCTCAA GGACTTCAAT ATTCCTCAGG CTGGAGGCTG GGGTCCCTGG 
GGACCATGGG GTGACTGCTC CAGGACTTGT GGAGGTGGTG TCCAATTCTC CTCCCGGGAC 
TGCACAAAGC CTGTCCCCCG GAATGGTGGA AAGTATTGTG AGGGCCGCCG TACCCCGTTC 
CGCTCCTGTA ACACTAAGAA CTGCCCACAT GGCTCAGCAT TGACCTTCCG TGAAGAGCAG 
TGTGCTGCCT ACAACCACCG AACCGACCTC TTCAAGAGTT TTCCGGGGCC CATGGACTGG 
GTTCCGCGCT ACACAGGTGT AGCCCCTCGA GACCAGTGCA AACTCACCTG CCAGGCCCGG 
GCACTGGGCT ACTACTATGT ACTGGAGCCA CGGGTGGCAG ATGGAACTCC CTGTTCCCCA 
GACTCTTCCT CTGTCTGTGT CCAGGGCCGC TGTATCCACG CTGGCTGTGA TCGAATCATT 
GGCTCCAAAA AGAAATTTGA CAAGTGCATG GTGTGCGGTG GGAATGGCTC TAGCTGCAGC 
AAGCAGTCAG GCTCCTTCAA AAAATTCAGG TACGGATACA GCGATGTGGT CACGATCCCT 
GCGGGACGCA CCCATATCCT TGTACGGCAG CAGGGAGGAT CTGGCCTCAA GAGCATCTAC 
CTGGCCCTGA AGCTAGCAGA TGGTTCTTAC GCCCTCAATG GTGAATACAC GCTGATGCCC 
TCCTCAACAG ATGTGGTACT TCCTGGGGCA GTCAGCTTGC GCTATAGCGG ACGCACGGCA 
GCCTCAGAGA CACTGTCTGG ACATGGGCCG CTGGCCCAGC CCTTGACGCT GCAAGTTCTG 
GTGGCTGGCA ACCCACAGAA TGTACGTCTG CGGTACAGTT TCTTTGTCCC GCGGCCAGTC 
CCTTCAACGC CACGCCCTCC TCCCCAAAAC TGGCTGCAAC GCAGGGCAGA GATTCTGGAG 
ATCCTTCGGA AGCGAACCTG GGCAGGCCGG AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Comparative proteomic analysis of supportive and unsupportive extracellular matrix substrates for human embryonic stem cell maintenance.
The Journal of biological chemistry288(26)18716-31(2013 Jun)
Soteriou D,Iskender B,Byron A,Humphries JD,Borg-Bartolo S,Haddock MC,Baxter MA,Knight D,Humphries MJ,Kimber SJ

Cochlin produced by follicular dendritic cells promotes antibacterial innate immunity.
Immunity38(5)1063-72(2013 May)
Py BF,Gonzalez SF,Long K,Kim MS,Kim YA,Zhu H,Yao J,Degauque N,Villet R,Ymele-Leki P,Gadjeva M,Pier GB,Carroll MC,Yuan J

Rat tail static compression model mimics extracellular matrix metabolic imbalances of matrix metalloproteinases, aggrecanases, and tissue inhibitors of metalloproteinases in intervertebral disc degeneration.
Arthritis research & therapy14(2)R51(2012 Mar)
Yurube T,Takada T,Suzuki T,Kakutani K,Maeno K,Doita M,Kurosaka M,Nishida K

Targeting bone alleviates osteoarthritis in osteopenic mice and modulates cartilage catabolism.
PloS one7(3)e33543(2012)
Funck-Brentano T,Lin H,Hay E,Ah Kioon MD,Schiltz C,Hannouche D,Nizard R,Liot? F,Orcel P,de Vernejoul MC,Cohen-Solal ME

The quantification of ADAMTS4 and 8 expression and selection of reference genes for quantitative real-time PCR analysis in myocardial infarction.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie65(8)555-9(2011 Dec)
Zhao C,Zha Y,Wu X,Chen L,Shi J,Cui L

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Adamts4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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