Rem2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rem2
Entrez Gene ID	64626
Full Name	RRAD and GEM like GTPase 2
General protein information	Preferred Names RRAD and GEM like GTPase 2 Names GTP-binding protein REM 2 GEM, REM, Kir family small G-protein GTP-binding protein REM2 RAS (RAD and GEM) like GTP binding 2 Ras-related GTP-binding protein of the Rad/Gem/Kir family, member 2 rad and Gem-like GTP-binding protein 2 rad and gem related GTP binding protein 2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 15 Map Location: 15p13
Summary	a Ras-related small GTP-binding protein that is found in high levels in neuronal tissues [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_022685.2	NP_073176.2	GTP-binding protein REM 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	REM2	NP_775798.2
Canis lupus familiaris (dog)	REM2	XP_005623273.1
Bos taurus (cattle)	REM2	NP_001179637.1
Xenopus tropicalis (tropical clawed frog)	rem2	XP_002941555.1
Pan troglodytes (chimpanzee)	REM2	XP_522796.2
	LOC712572	XP_001100692.1
Mus musculus (house mouse)	Rem2	NP_542764.2
Rattus norvegicus (Norway rat)	Rem2	NP_073176.2

Gene Ontology
Bibliography

Related articles in PubMed

Maternal care associates with differences in morphological complexity in the medial preoptic area.
Parent C, Wen X, Dhir SK, Ryan R, Diorio J, Zhang TY
Behavioural brain research32622-32(2017 May)

Rem2 is an activity-dependent negative regulator of dendritic complexity in vivo.
Ghiretti AE, Moore AR, Brenner RG, Chen LF, West AE, Lau NC, Van Hooser SD, Paradis S
The Journal of neuroscience : the official journal of the Society for Neuroscience34(2)392-407(2014 Jan)

Structure of the GDP-bound G domain of the RGK protein Rem2.
Reymond P, Coquard A, Chenon M, Zeghouf M, El Marjou A, Thompson A, M?n?trey J
Acta crystallographica. Section F, Structural biology and crystallization communications68(Pt 6)626-31(2012 Jun)

Activity-dependent subcellular cotrafficking of the small GTPase Rem2 and Ca2+/CaM-dependent protein kinase II?.
Flynn R, Labrie-Dion E, Bernier N, Colicos MA, De Koninck P, Zamponi GW
PloS one7(7)e41185(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Rem2 is regulated at the transcriptional level by calcium influx and inhibits dendritic arborization in cultured rat cortical neurons
Title: Rem2 is an activity-dependent negative regulator of dendritic complexity in vivo.

structure of the Rem2 G domain bound to GDP is reported here in a monoclinic crystal form at 2.66 A resolution
Title: Structure of the GDP-bound G domain of the RGK protein Rem2.

Rem2 has a role in neuronal plasticity hrough co-trafficking with CaMKIIa
Title: Activity-dependent subcellular cotrafficking of the small GTPase Rem2 and Ca2+/CaM-dependent protein kinase II?.

our results suggest that Rem2 regulates dendritic branching and synapse development
Title: The GTPase Rem2 regulates synapse development and dendritic morphology.

molecular mechanisms by which Rem2 knockdown affected synaptogenesis and Ca(2) homeostasis in cultured rat hippocampal neurons
Title: Rem2-targeted shRNAs reduce frequency of miniature excitatory postsynaptic currents without altering voltage-gated Ca?? currents.

The following Rem2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rem2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12168	NM_022685.2 Latest version!	Rattus norvegicus RRAD and GEM like GTPase 2 (Rem2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rem2 ( NM_022685.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_073176.2 Rattus norvegicus RRAD and GEM like GTPase 2 (Rem2), mRNA.

Price & Availability↑

CloneID	ORa12168
Clone ID Related Accession (Same CDS sequence)	NM_022685.2
Accession Version	NM_022685.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1026bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Rattus norvegicus(Norway rat)
Product	GTP-binding protein REM 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CB706504.1 and BC127495.1. On Sep 25, 2009 this sequence version replaced NM_022685.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC127495.1, AY916790.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCACACGG ACCTTGACAC CGACATGGAC GCGGACACCG AAACCGTAGC ACTTTGTTCT 
TCTAGCAGCC GCCAGGCCTC CCCATCAGGG ACACCCACAC CAGAAGCCGA TACGACACTT 
CTGAAGCAGA AGCCAGAGAA ACTGTTGGCA GAGTTGGACA GAGGTGGGCC ACCTCCTGCT 
CCTGGGGTCC CCAGACGAAG AGGAAGTATG CCTGTGCCCT ACAAACACCA GCTGCGGCGG 
GCTCAAGCTG TGGATGAGCT TGACTGGCCA CCTCAGGCCT CTTCCTCTGG CTCCTCTGAC 
TCGTTGGGCT CAGGGGAGGC AGCCCTGGCC CAAAAAGATG GTGTCTTTAA GGTCATGCTC 
CTGGGAGAGA GTGGCGTGGG CAAGAGCACT CTAGCAGGCA CTTTCGGAGG TCTCCAGGGA 
GACAATGCTC ACGAGATGGA GAACTCAGAG GACACCTATG AGAGACGGAT CATGGTGGAC 
AAAGAAGAAG TGACTTTAAT TGTTTATGAC ATCTGGGAAC AGGGAGACGC AGGAGGATGG 
CTGCAGGACC ACTGCCTTCA GACGGGGGAC GCCTTTCTCA TCGTCTTTTC AGTCACAGAT 
CGACGAAGCT TCTCCAAAGT TCCTGAAACC CTTCTTCGAC TCCGGGCTGG GAGGCCCCAC 
CACGACCTAC CTGTCATCCT TGTTGGAAAT AAGAGTGACC TGGCCCGTTC CCGGGAGGTG 
TCACTGGAGG AGGGTCGCCA TCTGGCCGGG ACGCTGAGCT GCAAGCACAT AGAGACATCA 
GCCGCTCTCC ACCACAACAC TCGTGAGCTC TTCGAGGGCG CTGTGCGTCA GATCAGGCTG 
CGGCGGGGCC GGGGTCATGC CGGGGGCCAG CGGCCGGAAC CTAGCAGCCC GGACGGCCCC 
GCGCCGCCTA CGCGCCGCGA GAGCCTCACC AAGAAAGCTA AGCGCTTCCT CGCCAACCTG 
GTGCCGCGCA ACGCCAAGTT CTTCAAGCAA CGCTCCAGGT CGTGTCACGA CCTCTCTGTG 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_073176.2
CDS	85..1110
Translation	MHTDLDTDMDADTETVALCSSSSRQASPSGTPTPEADTTLLKQKPEKLLAELDRGGPPPAPGVPRRRGSMPVPYKHQLRRAQAVDELDWPPQASSSGSSDSLGSGEAALAQKDGVFKVMLLGESGVGKSTLAGTFGGLQGDNAHEMENSEDTYERRIMVDKEEVTLIVYDIWEQGDAGGWLQDHCLQTGDAFLIVFSVTDRRSFSKVPETLLRLRAGRPHHDLPVILVGNKSDLARSREVSLEEGRHLAGTLSCKHIETSAALHHNTRELFEGAVRQIRLRRGRGHAGGQRPEPSSPDGPAPPTRRESLTKKAKRFLANLVPRNAKFFKQRSRSCHDLSVL

Target ORF information:

RefSeq Version	NM_022685.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus RRAD and GEM like GTPase 2 (Rem2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022685.2

ORF Insert Sequence:

ATGCACACGG ACCTTGACAC CGACATGGAC GCGGACACCG AAACCGTAGC ACTTTGTTCT 
TCTAGCAGCC GCCAGGCCTC CCCATCAGGG ACACCCACAC CAGAAGCCGA TACGACACTT 
CTGAAGCAGA AGCCAGAGAA ACTGTTGGCA GAGTTGGACA GAGGTGGGCC ACCTCCTGCT 
CCTGGGGTCC CCAGACGAAG AGGAAGTATG CCTGTGCCCT ACAAACACCA GCTGCGGCGG 
GCTCAAGCTG TGGATGAGCT TGACTGGCCA CCTCAGGCCT CTTCCTCTGG CTCCTCTGAC 
TCGTTGGGCT CAGGGGAGGC AGCCCTGGCC CAAAAAGATG GTGTCTTTAA GGTCATGCTC 
CTGGGAGAGA GTGGCGTGGG CAAGAGCACT CTAGCAGGCA CTTTCGGAGG TCTCCAGGGA 
GACAATGCTC ACGAGATGGA GAACTCAGAG GACACCTATG AGAGACGGAT CATGGTGGAC 
AAAGAAGAAG TGACTTTAAT TGTTTATGAC ATCTGGGAAC AGGGAGACGC AGGAGGATGG 
CTGCAGGACC ACTGCCTTCA GACGGGGGAC GCCTTTCTCA TCGTCTTTTC AGTCACAGAT 
CGACGAAGCT TCTCCAAAGT TCCTGAAACC CTTCTTCGAC TCCGGGCTGG GAGGCCCCAC 
CACGACCTAC CTGTCATCCT TGTTGGAAAT AAGAGTGACC TGGCCCGTTC CCGGGAGGTG 
TCACTGGAGG AGGGTCGCCA TCTGGCCGGG ACGCTGAGCT GCAAGCACAT AGAGACATCA 
GCCGCTCTCC ACCACAACAC TCGTGAGCTC TTCGAGGGCG CTGTGCGTCA GATCAGGCTG 
CGGCGGGGCC GGGGTCATGC CGGGGGCCAG CGGCCGGAAC CTAGCAGCCC GGACGGCCCC 
GCGCCGCCTA CGCGCCGCGA GAGCCTCACC AAGAAAGCTA AGCGCTTCCT CGCCAACCTG 
GTGCCGCGCA ACGCCAAGTT CTTCAAGCAA CGCTCCAGGT CGTGTCACGA CCTCTCTGTG 
CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Maternal care associates with differences in morphological complexity in the medial preoptic area.
Behavioural brain research32622-32(2017 May)
Parent C,Wen X,Dhir SK,Ryan R,Diorio J,Zhang TY

Rem2 is an activity-dependent negative regulator of dendritic complexity in vivo.
The Journal of neuroscience : the official journal of the Society for Neuroscience34(2)392-407(2014 Jan)
Ghiretti AE,Moore AR,Brenner RG,Chen LF,West AE,Lau NC,Van Hooser SD,Paradis S

Structure of the GDP-bound G domain of the RGK protein Rem2.
Acta crystallographica. Section F, Structural biology and crystallization communications68(Pt 6)626-31(2012 Jun)
Reymond P,Coquard A,Chenon M,Zeghouf M,El Marjou A,Thompson A,M?n?trey J

Activity-dependent subcellular cotrafficking of the small GTPase Rem2 and Ca2+/CaM-dependent protein kinase II?.
PloS one7(7)e41185(2012)
Flynn R,Labrie-Dion E,Bernier N,Colicos MA,De Koninck P,Zamponi GW