Arhgef1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Arhgef1
Entrez Gene ID	60323
Full Name	Rho guanine nucleotide exchange factor 1
Synonyms	Lsc
General protein information	Preferred Names Rho guanine nucleotide exchange factor 1 Names rho guanine nucleotide exchange factor 1 Rho guanine nucleotide exchange factor (GEF) 1 lbc's second cousin
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q21
Summary	mouse homolog is an oncogene with similarity to the Dbl family of guanine nucleotide exchange factors [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_021694.3	NP_067726.3	rho guanine nucleotide exchange factor 1

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	arhgef1	XP_002941974.2
Canis lupus familiaris (dog)	ARHGEF1	XP_005616572.1
Canis lupus familiaris (dog)	LOC100856005	XP_003640357.2
Pan troglodytes (chimpanzee)	ARHGEF1	XP_003953551.1
Mus musculus (house mouse)	Arhgef1	NP_032514.1
Rattus norvegicus (Norway rat)	Arhgef1	NP_067726.3
Homo sapiens (human)	ARHGEF1	NP_945353.1
Bos taurus (cattle)	ARHGEF1	XP_005219451.1

Gene Ontology
Bibliography

Related articles in PubMed

ROS-dependent activation of RhoA/Rho-kinase in pulmonary artery: Role of Src-family kinases and ARHGEF1.
MacKay CE, Shaifta Y, Snetkov VV, Francois AA, Ward JPT, Knock GA
Free radical biology & medicine110316-331(2017 Sep)

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

Mutant huntingtin alters cell fate in response to microtubule depolymerization via the GEF-H1-RhoA-ERK pathway.
Varma H, Yamamoto A, Sarantos MR, Hughes RE, Stockwell BR
The Journal of biological chemistry285(48)37445-57(2010 Nov)

Endothelin-1 up-regulates p115RhoGEF in embryonic rat cardiomyocytes during the hypertrophic response.
Porchia F, Papucci M, Gargini C, Asta A, De Marco G, Agretti P, Tonacchera M, Mazzoni MR
Journal of receptor and signal transduction research28(3)265-83(2008)

A novel role for Lsc/p115 RhoGEF and LARG in regulating RhoA activity downstream of adhesion to fibronectin.
Dubash AD, Wennerberg K, Garc?a-Mata R, Menold MM, Arthur WT, Burridge K
Journal of cell science120(Pt 22)3989-98(2007 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

results indicate that ROCK and its downstream target molecules, which are involved in inducing the hypertrophic response, are also implicated in signaling the up-regulation of the p115RhoGEF protein.
Title: Endothelin-1 up-regulates p115RhoGEF in embryonic rat cardiomyocytes during the hypertrophic response.

These data establish a previously uncharacterized role for the exchange factors Lsc/p115 RhoGEF and LARG in linking fibronectin signals to downstream RhoA activation.
Title: A novel role for Lsc/p115 RhoGEF and LARG in regulating RhoA activity downstream of adhesion to fibronectin.

The following Arhgef1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Arhgef1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa05084	NM_021694.3 Latest version!	Rattus norvegicus Rho guanine nucleotide exchange factor 1 (Arhgef1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$538.30 $769.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Arhgef1 ( NM_021694.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_067726.3 Rattus norvegicus Rho guanine nucleotide exchange factor 1 (Arhgef1), mRNA.

Price & Availability↑

CloneID	ORa05084
Clone ID Related Accession (Same CDS sequence)	NM_021694.3
Accession Version	NM_021694.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2760bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	rho guanine nucleotide exchange factor 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC091218.1. On Apr 20, 2005 this sequence version replaced NM_021694.2. ##Evidence-Data-START## Transcript exon combination :: BC091218.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906348, SAMN02642653 [ECO:0000348] ##Evidence-Data-END##

ATGGGAGAAG TCGCCGGAGG GGCGGCCCCA GGGCCTCCCC GGTCTGGCCT GGTGTCCATC 
ATCATCGGGG CTGAGGATGA GGACTTTGAG AATGAACTGG AGGCGAACCC AGAAGATCAA 
AACAGCCAGT TCCAGAGCCT GGAGCAAGTG AAGCGTCGCC CAGCCCACCT CATGGCCCTC 
CTGCAACACG TGGCCCTGCA GTTCGAGCCA GGACCACTGC TCTGCTGCCT GCATGCTGAC 
ATGCTAAGCT CACTGGGCCC CAAAGAAGCC AAGAAGGCCT TCCTCGATTT CTATCACAGT 
TTCCTGGAGA AGACTGCGGT TCTGCGGGTG CCGGTCCCTC CCAGCGTTGC TTTTGAACTT 
GATCGTACTC GGCCTGATCT CATCTCTGAG GATGTCCAGA GGCGGTTCAT ACAAGAGGTG 
GTACAGAGCC AGCAGGCAGC CGTGACCCGT CAGCTAGAGG ACTTCCGCTC CAAACGGCTC 
ATGGGCATGA CACCCTGGGA GCAGGAACTG AGCCTGCTAG AGCCCTGGAT TGGGAAAGAC 
CGGGGCAACT ATGAGGCCCG GGAGCGGCAT GTTGCAGAGC GGCTGCTCTC CCACCTAGAG 
GAAATGCAAC ATACCATCTC CACAGATGAA GAAAAAAGTG CTGCTGTGGT CACTGCAATC 
AGCCTGTATA TGCGCCACCT TGGGGTCCGG ACCAAGAGTG GGGACAAGAA GTCAGGGAGG 
AACTTCTTCC GGAAAAAGGT GATGGGGAAT CGGCGGTCAG ACGAGCCCCC CAAGACAAAG 
AAAGGGCTGA GCAGTATCCT AGATCCTGCC CGCTGGAACC GGGGAGAGCC ATCTGCTCCA 
GACTGTCGAC ATCTAAAGGT CGAGGTTGAT GAGAAGCCAG GCCCTGCAGA CCGGAAGGGA 
AGCCTGGGTA TTTCATCTCG GGACAGGACT GTTGGGACTC CTGGGCAGGA CAACCCAGGA 
GTCTCCCTGC ACCCTCTGTC TGTAGACAGC CTGGATTCCC GGGAACCAGG TGTGGATACC 
CCGCAGGAGC CAGGAGATAC ACCCCCACAA GGCCCTACAA GCCTGGAGCC CCTGGCACCC 
CCAGAGAGCA CAGAGGACAA TGGCGAGACT GAAAGCCCTG AGCCTGGAGA TGATGGAGAG 
CCAGGGCGGT CAGGCCTGGA ACAGGAACCA GAAGAACCTC CTGGGTGGAG GGAACTGGTG 
CCCTCAGACA CCCTGCTCGG CCTGCCCAAG AACCAAGTGA AGCGGCAAGA GGTCATCAGT 
GAGCTGCTGG TGACGGAGGC GGCTCACGTC CGCATGCTAC GGGTACTACA TGACCTCTTC 
TACCAGCCCA TGGCAGAAGG GGGCTTCTTC CCTCTGGAGG AACTGCAGAA CATCTTTCCC 
AGCCTGGATG AGCTCATCGA GGTGCACTCC CTGTTCCTCG ATCGCTTGAT GAAGCGGAGA 
CAAGAGAGTG GCTACCTCAT TGAGGAGATT GGTGATGTGC TACTGGCCCG GTTCGATGGT 
GCTGAGGGCT CATGGTTCCA GAAGATCTCT TCCCGCTTCT GCAGCCGCCA GTCATTCGCT 
CTAGAGCAGC TCAAAGCCAA GCAGCGCAAG GAGCCTCGAT TCTGTGCCTT TGTGCAGGAA 
GCTGAGAGCC GCCCCCGCTG CCGGCGGCTG CAGTTGAAGG ACATGATCCC CACTGAGATG 
CAGCGGCTGA CCAAGTACCC ACTGCTGCTG CAGAGTATTG GGCAGAACAC AGAAGAGCCT 
GCCGAGCGGG CAAAGGTGGA ACTTGCAGCT GAGTGCTGCC GTGAGATTCT GCACCACGTT 
AATCAAGCTG TCCGTGACAT GGAGGACCTG CTGCGGCTCA AGGATTACCA GCGGCGCCTG 
GACTTGACTC ACCTGCGGCA GAGCAATGAC CCAATGCTGA GCGAGTTCAA GAACCTGGAT 
ATCACTAAGA AGAAGTTGGT CCATGAAGGC CCCCTCACGT GGCGACTAAC CAAAGACAAA 
GCTGTAGAGG TCCACGTGCT ATTGCTGGAC GACCTGCTGC TGCTGCTCCA GCGCCAGGAT 
GAGAGGCTGC TGCTCAAGTC ACACAGCCGG ACGCTGACAC CCACACCCGA CGGCAAGACC 
ATGCTGCGGC CTGTGCTCCG GCTCACCTCT GCCATGACCC GGGAGGTGGC CACTGATCAC 
AAAGCTTTTT ACGTCATTTT TACCTGGGAC CAGGAGGCCC AGATATATGA GCTGGTGGCA 
CAGACATCGT CGGAACGCAA GAGCTGGTGT GCCCTCATCA CAGAGACTGC CGGATCCCTG 
AAGGTCCCTG CCCCTGCCTC CCGCCCCAAA CCCCGGCCCA GCCCAAGCAG CACCCGAGAA 
CCCCTGCTCA GCAGCTCTGA GAATGGCACT GGAGGCACAG AGGCCGCTCC AGCTGATGCC 
CGGACAGAGC GGATCCTCAA CGACCTCCTG CCCTTCTGCA GACCAGGCCC AGAGGGCCAG 
CTTGCTGCCA CAGCCCTCCA GAAAGTACTG TCCCTGAAAC AGATCCTGTT GAGCACTGAG 
GAAGACAGTG GAGCAGGGCC TCCCCGCGAT GGGGATGGGG TGCCTGGTGG TGGTGCCCCA 
GGCCCAACAC ACACCCAGGA GGTTGAGGAA AACCTGTTGA GCCTAGAAGT GGTCATCAAA 
CAACTGGAGG AGTTAGAAGA GGAATTTTGT CGCCTAAGAC CCTTCCTGTC TCAGCTTGGG 
GAGATTCTGT CCCCCAACCT GGCTGCACCT GAGCGCTCTG CTCAGACAGG CCTTTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_067726.3
CDS	114..2873
Translation	MGEVAGGAAPGPPRSGLVSIIIGAEDEDFENELEANPEDQNSQFQSLEQVKRRPAHLMALLQHVALQFEPGPLLCCLHADMLSSLGPKEAKKAFLDFYHSFLEKTAVLRVPVPPSVAFELDRTRPDLISEDVQRRFIQEVVQSQQAAVTRQLEDFRSKRLMGMTPWEQELSLLEPWIGKDRGNYEARERHVAERLLSHLEEMQHTISTDEEKSAAVVTAISLYMRHLGVRTKSGDKKSGRNFFRKKVMGNRRSDEPPKTKKGLSSILDPARWNRGEPSAPDCRHLKVEVDEKPGPADRKGSLGISSRDRTVGTPGQDNPGVSLHPLSVDSLDSREPGVDTPQEPGDTPPQGPTSLEPLAPPESTEDNGETESPEPGDDGEPGRSGLEQEPEEPPGWRELVPSDTLLGLPKNQVKRQEVISELLVTEAAHVRMLRVLHDLFYQPMAEGGFFPLEELQNIFPSLDELIEVHSLFLDRLMKRRQESGYLIEEIGDVLLARFDGAEGSWFQKISSRFCSRQSFALEQLKAKQRKEPRFCAFVQEAESRPRCRRLQLKDMIPTEMQRLTKYPLLLQSIGQNTEEPAERAKVELAAECCREILHHVNQAVRDMEDLLRLKDYQRRLDLTHLRQSNDPMLSEFKNLDITKKKLVHEGPLTWRLTKDKAVEVHVLLLDDLLLLLQRQDERLLLKSHSRTLTPTPDGKTMLRPVLRLTSAMTREVATDHKAFYVIFTWDQEAQIYELVAQTSSERKSWCALITETAGSLKVPAPASRPKPRPSPSSTREPLLSSSENGTGGTEAAPADARTERILNDLLPFCRPGPEGQLAATALQKVLSLKQILLSTEEDSGAGPPRDGDGVPGGGAPGPTHTQEVEENLLSLEVVIKQLEELEEEFCRLRPFLSQLGEILSPNLAAPERSAQTGLS

Target ORF information:

RefSeq Version	NM_021694.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus Rho guanine nucleotide exchange factor 1 (Arhgef1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021694.3

ORF Insert Sequence:

ATGGGAGAAG TCGCCGGAGG GGCGGCCCCA GGGCCTCCCC GGTCTGGCCT GGTGTCCATC 
ATCATCGGGG CTGAGGATGA GGACTTTGAG AATGAACTGG AGGCGAACCC AGAAGATCAA 
AACAGCCAGT TCCAGAGCCT GGAGCAAGTG AAGCGTCGCC CAGCCCACCT CATGGCCCTC 
CTGCAACACG TGGCCCTGCA GTTCGAGCCA GGACCACTGC TCTGCTGCCT GCATGCTGAC 
ATGCTAAGCT CACTGGGCCC CAAAGAAGCC AAGAAGGCCT TCCTCGATTT CTATCACAGT 
TTCCTGGAGA AGACTGCGGT TCTGCGGGTG CCGGTCCCTC CCAGCGTTGC TTTTGAACTT 
GATCGTACTC GGCCTGATCT CATCTCTGAG GATGTCCAGA GGCGGTTCAT ACAAGAGGTG 
GTACAGAGCC AGCAGGCAGC CGTGACCCGT CAGCTAGAGG ACTTCCGCTC CAAACGGCTC 
ATGGGCATGA CACCCTGGGA GCAGGAACTG AGCCTGCTAG AGCCCTGGAT TGGGAAAGAC 
CGGGGCAACT ATGAGGCCCG GGAGCGGCAT GTTGCAGAGC GGCTGCTCTC CCACCTAGAG 
GAAATGCAAC ATACCATCTC CACAGATGAA GAAAAAAGTG CTGCTGTGGT CACTGCAATC 
AGCCTGTATA TGCGCCACCT TGGGGTCCGG ACCAAGAGTG GGGACAAGAA GTCAGGGAGG 
AACTTCTTCC GGAAAAAGGT GATGGGGAAT CGGCGGTCAG ACGAGCCCCC CAAGACAAAG 
AAAGGGCTGA GCAGTATCCT AGATCCTGCC CGCTGGAACC GGGGAGAGCC ATCTGCTCCA 
GACTGTCGAC ATCTAAAGGT CGAGGTTGAT GAGAAGCCAG GCCCTGCAGA CCGGAAGGGA 
AGCCTGGGTA TTTCATCTCG GGACAGGACT GTTGGGACTC CTGGGCAGGA CAACCCAGGA 
GTCTCCCTGC ACCCTCTGTC TGTAGACAGC CTGGATTCCC GGGAACCAGG TGTGGATACC 
CCGCAGGAGC CAGGAGATAC ACCCCCACAA GGCCCTACAA GCCTGGAGCC CCTGGCACCC 
CCAGAGAGCA CAGAGGACAA TGGCGAGACT GAAAGCCCTG AGCCTGGAGA TGATGGAGAG 
CCAGGGCGGT CAGGCCTGGA ACAGGAACCA GAAGAACCTC CTGGGTGGAG GGAACTGGTG 
CCCTCAGACA CCCTGCTCGG CCTGCCCAAG AACCAAGTGA AGCGGCAAGA GGTCATCAGT 
GAGCTGCTGG TGACGGAGGC GGCTCACGTC CGCATGCTAC GGGTACTACA TGACCTCTTC 
TACCAGCCCA TGGCAGAAGG GGGCTTCTTC CCTCTGGAGG AACTGCAGAA CATCTTTCCC 
AGCCTGGATG AGCTCATCGA GGTGCACTCC CTGTTCCTCG ATCGCTTGAT GAAGCGGAGA 
CAAGAGAGTG GCTACCTCAT TGAGGAGATT GGTGATGTGC TACTGGCCCG GTTCGATGGT 
GCTGAGGGCT CATGGTTCCA GAAGATCTCT TCCCGCTTCT GCAGCCGCCA GTCATTCGCT 
CTAGAGCAGC TCAAAGCCAA GCAGCGCAAG GAGCCTCGAT TCTGTGCCTT TGTGCAGGAA 
GCTGAGAGCC GCCCCCGCTG CCGGCGGCTG CAGTTGAAGG ACATGATCCC CACTGAGATG 
CAGCGGCTGA CCAAGTACCC ACTGCTGCTG CAGAGTATTG GGCAGAACAC AGAAGAGCCT 
GCCGAGCGGG CAAAGGTGGA ACTTGCAGCT GAGTGCTGCC GTGAGATTCT GCACCACGTT 
AATCAAGCTG TCCGTGACAT GGAGGACCTG CTGCGGCTCA AGGATTACCA GCGGCGCCTG 
GACTTGACTC ACCTGCGGCA GAGCAATGAC CCAATGCTGA GCGAGTTCAA GAACCTGGAT 
ATCACTAAGA AGAAGTTGGT CCATGAAGGC CCCCTCACGT GGCGACTAAC CAAAGACAAA 
GCTGTAGAGG TCCACGTGCT ATTGCTGGAC GACCTGCTGC TGCTGCTCCA GCGCCAGGAT 
GAGAGGCTGC TGCTCAAGTC ACACAGCCGG ACGCTGACAC CCACACCCGA CGGCAAGACC 
ATGCTGCGGC CTGTGCTCCG GCTCACCTCT GCCATGACCC GGGAGGTGGC CACTGATCAC 
AAAGCTTTTT ACGTCATTTT TACCTGGGAC CAGGAGGCCC AGATATATGA GCTGGTGGCA 
CAGACATCGT CGGAACGCAA GAGCTGGTGT GCCCTCATCA CAGAGACTGC CGGATCCCTG 
AAGGTCCCTG CCCCTGCCTC CCGCCCCAAA CCCCGGCCCA GCCCAAGCAG CACCCGAGAA 
CCCCTGCTCA GCAGCTCTGA GAATGGCACT GGAGGCACAG AGGCCGCTCC AGCTGATGCC 
CGGACAGAGC GGATCCTCAA CGACCTCCTG CCCTTCTGCA GACCAGGCCC AGAGGGCCAG 
CTTGCTGCCA CAGCCCTCCA GAAAGTACTG TCCCTGAAAC AGATCCTGTT GAGCACTGAG 
GAAGACAGTG GAGCAGGGCC TCCCCGCGAT GGGGATGGGG TGCCTGGTGG TGGTGCCCCA 
GGCCCAACAC ACACCCAGGA GGTTGAGGAA AACCTGTTGA GCCTAGAAGT GGTCATCAAA 
CAACTGGAGG AGTTAGAAGA GGAATTTTGT CGCCTAAGAC CCTTCCTGTC TCAGCTTGGG 
GAGATTCTGT CCCCCAACCT GGCTGCACCT GAGCGCTCTG CTCAGACAGG CCTTTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

ROS-dependent activation of RhoA/Rho-kinase in pulmonary artery: Role of Src-family kinases and ARHGEF1.
Free radical biology & medicine110316-331(2017 Sep)
MacKay CE,Shaifta Y,Snetkov VV,Francois AA,Ward JPT,Knock GA

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

Mutant huntingtin alters cell fate in response to microtubule depolymerization via the GEF-H1-RhoA-ERK pathway.
The Journal of biological chemistry285(48)37445-57(2010 Nov)
Varma H,Yamamoto A,Sarantos MR,Hughes RE,Stockwell BR

Endothelin-1 up-regulates p115RhoGEF in embryonic rat cardiomyocytes during the hypertrophic response.
Journal of receptor and signal transduction research28(3)265-83(2008)
Porchia F,Papucci M,Gargini C,Asta A,De Marco G,Agretti P,Tonacchera M,Mazzoni MR

A novel role for Lsc/p115 RhoGEF and LARG in regulating RhoA activity downstream of adhesion to fibronectin.
Journal of cell science120(Pt 22)3989-98(2007 Nov)
Dubash AD,Wennerberg K,Garc?a-Mata R,Menold MM,Arthur WT,Burridge K

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Arhgef1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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