arhgef1 gene cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

mRNA	Protein	Name
XM_004916245.3	XP_004916302.2	rho guanine nucleotide exchange factor 1 isoform X7
XM_012967446.2	XP_012822900.1	rho guanine nucleotide exchange factor 1 isoform X8
XM_004916244.4	XP_004916301.3	rho guanine nucleotide exchange factor 1 isoform X6
XM_012967441.2	XP_012822895.2	rho guanine nucleotide exchange factor 1 isoform X1
XM_012967444.2	XP_012822898.2	rho guanine nucleotide exchange factor 1 isoform X4
XM_012967443.2	XP_012822897.1	rho guanine nucleotide exchange factor 1 isoform X3
XM_012967441.3	XP_012822895.3	rho guanine nucleotide exchange factor 1 isoform X1
XM_012967442.3	XP_012822896.3	rho guanine nucleotide exchange factor 1 isoform X2
XM_004916245.4	XP_004916302.3	rho guanine nucleotide exchange factor 1 isoform X7
XM_012967446.3	XP_012822900.2	rho guanine nucleotide exchange factor 1 isoform X8
XM_012967445.2	XP_012822899.2	rho guanine nucleotide exchange factor 1 isoform X5
XM_004916244.3	XP_004916301.2	rho guanine nucleotide exchange factor 1 isoform X6
XM_012967443.3	XP_012822897.2	rho guanine nucleotide exchange factor 1 isoform X3
XM_012967444.3	XP_012822898.3	rho guanine nucleotide exchange factor 1 isoform X4
XM_012967442.2	XP_012822896.2	rho guanine nucleotide exchange factor 1 isoform X2
XM_012967445.3	XP_012822899.3	rho guanine nucleotide exchange factor 1 isoform X5

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa45453	XM_012967441.2 Latest version!	Xenopus tropicalis Rho guanine nucleotide exchange factor 1 (arhgef1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OXa45454	XM_012967442.2 Latest version!	Xenopus tropicalis Rho guanine nucleotide exchange factor 1 (arhgef1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OXa45455	XM_012967444.2 Latest version!	Xenopus tropicalis Rho guanine nucleotide exchange factor 1 (arhgef1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OXa45456	XM_012967445.2 Latest version!	Xenopus tropicalis Rho guanine nucleotide exchange factor 1 (arhgef1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OXa62572	XM_012967441.3 Latest version!	Xenopus tropicalis Rho guanine nucleotide exchange factor 1 (arhgef1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa62573	XM_012967442.3 Latest version!	Xenopus tropicalis Rho guanine nucleotide exchange factor 1 (arhgef1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa62574	XM_012967443.3 Latest version!	Xenopus tropicalis Rho guanine nucleotide exchange factor 1 (arhgef1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa62575	XM_012967444.3 Latest version!	Xenopus tropicalis Rho guanine nucleotide exchange factor 1 (arhgef1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa62576	XM_012967445.3 Latest version!	Xenopus tropicalis Rho guanine nucleotide exchange factor 1 (arhgef1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa62577	XM_004916244.4 Latest version!	Xenopus tropicalis Rho guanine nucleotide exchange factor 1 (arhgef1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa62578	XM_004916245.4 Latest version!	Xenopus tropicalis Rho guanine nucleotide exchange factor 1 (arhgef1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa62579	XM_012967446.3 Latest version!	Xenopus tropicalis Rho guanine nucleotide exchange factor 1 (arhgef1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OXa29991	XM_012967443.2 Latest version!	Xenopus tropicalis Rho guanine nucleotide exchange factor 1 (arhgef1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OXa29994	XM_004916244.3 Latest version!	Xenopus tropicalis Rho guanine nucleotide exchange factor 1 (arhgef1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OXa29995	XM_004916245.3 Latest version!	Xenopus tropicalis Rho guanine nucleotide exchange factor 1 (arhgef1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OXa29996	XM_012967446.2 Latest version!	Xenopus tropicalis Rho guanine nucleotide exchange factor 1 (arhgef1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

arhgef1 ( XM_012967441.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012822895.2 Xenopus tropicalis Rho guanine nucleotide exchange factor 1 (arhgef1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa45453
Clone ID Related Accession (Same CDS sequence)	XM_012967441.2
Accession Version	XM_012967441.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3408bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho guanine nucleotide exchange factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012967441.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATCAT CCCCCTTGGC CATGACTCAC TCTGTCTACC TCTTACCCTC CTGCATGAGT 
TATTGTGGAT TCCAAAAGCA CGGCTCACAG GTTTCTCCTG GTCTGTGCAT CACTTATGCA 
TCAGCCGACC TGAGCAGGGG ATTCCCATTG TCTCACTCAC TGGGTTTTTG CCAGACTGCT 
CCCAAACCCC TCGTCTGCCA GATAAGAAGC ATGGATCCGG ACGATGGCCA TTTTGGGGGT 
TTGTACTCCA AGGGGTCCAT GTCGATCATT GGAGCAGAGG ATGAGGATTT TGAGAATGAC 
ATTGAACCGA ATATGGTTGA TGAGAGCAGT TACTTCCGAA GCATTGAGCT AGTGAAAGAA 
CACCCGGCCT ACATTATGGT GTTCTTGCAG CACGTGGTTC TTCAGTTTGA CCCCAGCCCA 
CTGCTCTGTT TCCTGCATGC GGAGATGCTA AAGAACCTTT CAGCCAAAGA GCTTAAGAAA 
GTCTTCATGG ATTTTCACTA CACATTTCTG ATGAAAGGAG CGGTACTGCA TGTCAGTGTG 
CCAAACAGCG TATCCTTTGA ACTGGATCGG ACAAGGCCTG AGATGATCCC AGAAGAAACC 
CAGAGAAAAT ATGTCAAAGA CATTCAGAAG CACCAAACTG AGGCCATTTC CCAACAGCTG 
GAGGACTTTA GGGCCAAGCG AGTACTTGGT ATGACCCCTG GGGAACTGGA GCTGAGCGAG 
CTTGAATCCT ATCGAACCAA CGACCGCAGT ATCCTGGAAG CCAAAGAGAA GCAGTTGGCA 
GAGCAGCTCC TGGCACGGCT GGATGAGATG CACCTAACTA TTGCTCCAGA CGAGGAAAAA 
AGCTCTGCCA TCTTTTGTGC CATTGTAGCC TACATGAAGC ACCTGGGGGT GAAGACCAAG 
GCGGCGGATA GCAAAAAATC TAAAGGAATC TTCTTCAGGA AAAAGGTTTC TACAAATAAA 
AAGGATGAGC CTCTGAAAAC AAAGAAAACA TTCAGCATCT TGGATCCTAT GCGCTGGAAC 
CGAAATGATT CTCACTATGT TGACAGCAGA CCATCCAAAG TTGAGCCCGA AGCTGAGAAG 
CCAAACCCGG ACAGAAGGGT CAGCCAGGGG CAGAGAACTT TTGATAGGAG TGACAGCAGC 
ACAGCGAAAT CCAAGAACAG CGAAGGGCCT GATTCTGTGG TGTCTGTAAA TATCATCCCC 
CCTCCTAATG AGCCCAATGA TCCTGACCCA GCAGTGACTG ATGGGGCATT GTCTAATGAT 
GCATCTGAGG TCCTCAAAGG ATCCCCCACC ACTGAGCATT GCCCAGCAGA CCAGACTGAG 
GAAAGTGCAG AAAATGAAAG ACGAAAGAAA AGGCTCTCTG GTAAGCTGGG CCGCTCGGAG 
AGCCTACGAG TTTACGAGCG CAAGCGTTCC CAGCGCGGCT CCTCCAAAGC CAAGCAGACA 
CGTTCCCGTA GCGACGTTGA CCTGGAGGCA GCTGCCCGAG CCAACGACTT GCAGGGCACC 
ACTGGCAAGC TTGGGCCAAA GCCTGGCAAC TACACCCCAG AGCCTGGAGG GAGTTCAGAG 
GGCCCGCTGC CCTTTCTGCT TCCCCAGTCT GAGGAGACTG AGCCGCGCGT TCAGGAGGCC 
GAGAGCGACC CCCCAAACTG GAGGGAGCTC GTCCCCCCTG AAATACTCCT CAGGCTGAAG 
AAAAGCGAAA TCAAGCGGCA AGAGGTCATC AATGAGCTCT TCTTTACAGA GCACGCTCAT 
GTACGGATGC TTCGTGTGTT GCATGATGTC TTCTACATCC CCATGCAGAA TGGATGGATC 
CCCACCTTGG AGCTGGAGAA CATCTTTCCT AGCCTGGATG ACCTTATCGA GGCTCATGCT 
GTATTCCTGG ACACCTTGAA GCGTCTGCGC CTTGAGAATA ACTCAGTGAT CCAGGAAATA 
GGCACCATCT TGCTGAACCG GTTTGACGGC ACAGAGGGCA ACTGGTTTCA GAAGATTTCA 
TCTCGGTTCT GCAGCCGGCA GCCATTTGCC TTGGAACAGT TGAAAGCGAA ACAGAAGAGA 
GACCCTCGAT TTACGCAGTT TATTCAGGAA GCTGAGAGCA GCCCTCACTG CCGGCGGTTA 
CAACTAAAGG ACATTATACC ATTTGAGATG CAAAGGCTGA CCAAGTACCC GTTGCTGCTG 
CAGAACATCG CAAATCTAAC TGAGGAAGAC GAGGAGAAGC AGAGAGTGCA GAGGGCAGCA 
GAGTCTTGCA GGCAGATCCT AAACCATGTT AACCAGGAGG TGCGCGAGAT GGAGAATAAG 
ATGAAACTTG CAGATTACCA AAGACGCCTG GATGTTTCCA ACCTGAGGCA AATCAACGAC 
CCCTTATTTA ATGAATTTAA GAACATTGAT ATCAGCAAAA AGAATTTGAT ACATGAAGGA 
TCTTTGACAT GGAAAGTGAG CAGGGACAAG GCTTTTGATG TCCATGTCCT CCTCCTGGAT 
GATCTCTTGA TGCTGCTGCT GAAACAAGAT GAGCGGCTGG TACTGAAGTG CCAGAGTCGG 
ACTATTATTC CTGCTCCAGA CGGGAAGCTC ATGCTGAGCC CAATCATCAA GCTGAATATG 
GCCATGGTCA GAGAGGTGGC TACAGATAAG AATGCCTTTT TTGTGATTTT CAACTGGGAG 
GATCGAGCAC AGTTCTATGA ACTTGTGGCT CCAACACCCG CTGAGAAGAA AAACTGGTTT 
TTACTGATCA GCAACACTAC AGGCTTATTG GAACCCCCAG CTTCTTCCAA ACAGAGACAC 
AGATCTATCA CTCCTGGCAC AATGCCGCAG AGCCCCTCCG ACTTTGCAGA TCAGCTGCTG 
AGCAGCTCAG AAAATGGAGG CTCCCTGAAA GATTCAGTAT CCAAAGATGA AAAGGAAGAG 
TCGGTGGAGG ATGCATTGGA AGGTCAGGAA GAGAAGGACT TCAGCGGCCT TACAGAATAC 
CTCCTTGCCA GCAACATTGA TCTGAACCAA GATGCAGCAT CTGGAGGAGG CAATGTTGCC 
AGCGAGGCCT TGGAAGAAGT GTCGGTGCTA AAACGCCTGC TAGTGCGCAA TATCCGCCTC 
AGTGTGGATG CAGACACGCT CTCAACCCTG GAGACCTGGT CAGCGGGGGA AGAACCTGCA 
CTGGAGGCTT CTGAACAGGG AAGGGCCTCC AATGGGGATC GAGAGGCAGA GGATTCTGAA 
ATGGAATCTA CACAACAGGG AGAGAATGGC AGAAAGCAGA GTTCTGCAGA TGTTGCAGAG 
TCCCTGTGCG CACCCATTGT ACTGTCCAAA GAACAGTCGG AGGAGGTGAA GAGGCACATT 
CTGTCACTGG AGCAGAGGAT CCAGCAGCTT AAGCTGATCG AGGAAGATTA CAACAACCTG 
CACAGAACAA TCGCCAAATT CACTGTACCG AGGAGCAGCT TTACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012822895.2
CDS	8..3415
Translation	MESSPLAMTHSVYLLPSCMSYCGFQKHGSQVSPGLCITYASADLSRGFPLSHSLGFCQTAPKPLVCQIRSMDPDDGHFGGLYSKGSMSIIGAEDEDFENDIEPNMVDESSYFRSIELVKEHPAYIMVFLQHVVLQFDPSPLLCFLHAEMLKNLSAKELKKVFMDFHYTFLMKGAVLHVSVPNSVSFELDRTRPEMIPEETQRKYVKDIQKHQTEAISQQLEDFRAKRVLGMTPGELELSELESYRTNDRSILEAKEKQLAEQLLARLDEMHLTIAPDEEKSSAIFCAIVAYMKHLGVKTKAADSKKSKGIFFRKKVSTNKKDEPLKTKKTFSILDPMRWNRNDSHYVDSRPSKVEPEAEKPNPDRRVSQGQRTFDRSDSSTAKSKNSEGPDSVVSVNIIPPPNEPNDPDPAVTDGALSNDASEVLKGSPTTEHCPADQTEESAENERRKKRLSGKLGRSESLRVYERKRSQRGSSKAKQTRSRSDVDLEAAARANDLQGTTGKLGPKPGNYTPEPGGSSEGPLPFLLPQSEETEPRVQEAESDPPNWRELVPPEILLRLKKSEIKRQEVINELFFTEHAHVRMLRVLHDVFYIPMQNGWIPTLELENIFPSLDDLIEAHAVFLDTLKRLRLENNSVIQEIGTILLNRFDGTEGNWFQKISSRFCSRQPFALEQLKAKQKRDPRFTQFIQEAESSPHCRRLQLKDIIPFEMQRLTKYPLLLQNIANLTEEDEEKQRVQRAAESCRQILNHVNQEVREMENKMKLADYQRRLDVSNLRQINDPLFNEFKNIDISKKNLIHEGSLTWKVSRDKAFDVHVLLLDDLLMLLLKQDERLVLKCQSRTIIPAPDGKLMLSPIIKLNMAMVREVATDKNAFFVIFNWEDRAQFYELVAPTPAEKKNWFLLISNTTGLLEPPASSKQRHRSITPGTMPQSPSDFADQLLSSSENGGSLKDSVSKDEKEESVEDALEGQEEKDFSGLTEYLLASNIDLNQDAASGGGNVASEALEEVSVLKRLLVRNIRLSVDADTLSTLETWSAGEEPALEASEQGRASNGDREAEDSEMESTQQGENGRKQSSADVAESLCAPIVLSKEQSEEVKRHILSLEQRIQQLKLIEEDYNNLHRTIAKFTVPRSSFT

Target ORF information:

RefSeq Version	XM_012967441.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho guanine nucleotide exchange factor 1 (arhgef1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012967441.2

ORF Insert Sequence:

ATGGAATCAT CCCCCTTGGC CATGACTCAC TCTGTCTACC TCTTACCCTC CTGCATGAGT 
TATTGTGGAT TCCAAAAGCA CGGCTCACAG GTTTCTCCTG GTCTGTGCAT CACTTATGCA 
TCAGCCGACC TGAGCAGGGG ATTCCCATTG TCTCACTCAC TGGGTTTTTG CCAGACTGCT 
CCCAAACCCC TCGTCTGCCA GATAAGAAGC ATGGATCCGG ACGATGGCCA TTTTGGGGGT 
TTGTACTCCA AGGGGTCCAT GTCGATCATT GGAGCAGAGG ATGAGGATTT TGAGAATGAC 
ATTGAACCGA ATATGGTTGA TGAGAGCAGT TACTTCCGAA GCATTGAGCT AGTGAAAGAA 
CACCCGGCCT ACATTATGGT GTTCTTGCAG CACGTGGTTC TTCAGTTTGA CCCCAGCCCA 
CTGCTCTGTT TCCTGCATGC GGAGATGCTA AAGAACCTTT CAGCCAAAGA GCTTAAGAAA 
GTCTTCATGG ATTTTCACTA CACATTTCTG ATGAAAGGAG CGGTACTGCA TGTCAGTGTG 
CCAAACAGCG TATCCTTTGA ACTGGATCGG ACAAGGCCTG AGATGATCCC AGAAGAAACC 
CAGAGAAAAT ATGTCAAAGA CATTCAGAAG CACCAAACTG AGGCCATTTC CCAACAGCTG 
GAGGACTTTA GGGCCAAGCG AGTACTTGGT ATGACCCCTG GGGAACTGGA GCTGAGCGAG 
CTTGAATCCT ATCGAACCAA CGACCGCAGT ATCCTGGAAG CCAAAGAGAA GCAGTTGGCA 
GAGCAGCTCC TGGCACGGCT GGATGAGATG CACCTAACTA TTGCTCCAGA CGAGGAAAAA 
AGCTCTGCCA TCTTTTGTGC CATTGTAGCC TACATGAAGC ACCTGGGGGT GAAGACCAAG 
GCGGCGGATA GCAAAAAATC TAAAGGAATC TTCTTCAGGA AAAAGGTTTC TACAAATAAA 
AAGGATGAGC CTCTGAAAAC AAAGAAAACA TTCAGCATCT TGGATCCTAT GCGCTGGAAC 
CGAAATGATT CTCACTATGT TGACAGCAGA CCATCCAAAG TTGAGCCCGA AGCTGAGAAG 
CCAAACCCGG ACAGAAGGGT CAGCCAGGGG CAGAGAACTT TTGATAGGAG TGACAGCAGC 
ACAGCGAAAT CCAAGAACAG CGAAGGGCCT GATTCTGTGG TGTCTGTAAA TATCATCCCC 
CCTCCTAATG AGCCCAATGA TCCTGACCCA GCAGTGACTG ATGGGGCATT GTCTAATGAT 
GCATCTGAGG TCCTCAAAGG ATCCCCCACC ACTGAGCATT GCCCAGCAGA CCAGACTGAG 
GAAAGTGCAG AAAATGAAAG ACGAAAGAAA AGGCTCTCTG GTAAGCTGGG CCGCTCGGAG 
AGCCTACGAG TTTACGAGCG CAAGCGTTCC CAGCGCGGCT CCTCCAAAGC CAAGCAGACA 
CGTTCCCGTA GCGACGTTGA CCTGGAGGCA GCTGCCCGAG CCAACGACTT GCAGGGCACC 
ACTGGCAAGC TTGGGCCAAA GCCTGGCAAC TACACCCCAG AGCCTGGAGG GAGTTCAGAG 
GGCCCGCTGC CCTTTCTGCT TCCCCAGTCT GAGGAGACTG AGCCGCGCGT TCAGGAGGCC 
GAGAGCGACC CCCCAAACTG GAGGGAGCTC GTCCCCCCTG AAATACTCCT CAGGCTGAAG 
AAAAGCGAAA TCAAGCGGCA AGAGGTCATC AATGAGCTCT TCTTTACAGA GCACGCTCAT 
GTACGGATGC TTCGTGTGTT GCATGATGTC TTCTACATCC CCATGCAGAA TGGATGGATC 
CCCACCTTGG AGCTGGAGAA CATCTTTCCT AGCCTGGATG ACCTTATCGA GGCTCATGCT 
GTATTCCTGG ACACCTTGAA GCGTCTGCGC CTTGAGAATA ACTCAGTGAT CCAGGAAATA 
GGCACCATCT TGCTGAACCG GTTTGACGGC ACAGAGGGCA ACTGGTTTCA GAAGATTTCA 
TCTCGGTTCT GCAGCCGGCA GCCATTTGCC TTGGAACAGT TGAAAGCGAA ACAGAAGAGA 
GACCCTCGAT TTACGCAGTT TATTCAGGAA GCTGAGAGCA GCCCTCACTG CCGGCGGTTA 
CAACTAAAGG ACATTATACC ATTTGAGATG CAAAGGCTGA CCAAGTACCC GTTGCTGCTG 
CAGAACATCG CAAATCTAAC TGAGGAAGAC GAGGAGAAGC AGAGAGTGCA GAGGGCAGCA 
GAGTCTTGCA GGCAGATCCT AAACCATGTT AACCAGGAGG TGCGCGAGAT GGAGAATAAG 
ATGAAACTTG CAGATTACCA AAGACGCCTG GATGTTTCCA ACCTGAGGCA AATCAACGAC 
CCCTTATTTA ATGAATTTAA GAACATTGAT ATCAGCAAAA AGAATTTGAT ACATGAAGGA 
TCTTTGACAT GGAAAGTGAG CAGGGACAAG GCTTTTGATG TCCATGTCCT CCTCCTGGAT 
GATCTCTTGA TGCTGCTGCT GAAACAAGAT GAGCGGCTGG TACTGAAGTG CCAGAGTCGG 
ACTATTATTC CTGCTCCAGA CGGGAAGCTC ATGCTGAGCC CAATCATCAA GCTGAATATG 
GCCATGGTCA GAGAGGTGGC TACAGATAAG AATGCCTTTT TTGTGATTTT CAACTGGGAG 
GATCGAGCAC AGTTCTATGA ACTTGTGGCT CCAACACCCG CTGAGAAGAA AAACTGGTTT 
TTACTGATCA GCAACACTAC AGGCTTATTG GAACCCCCAG CTTCTTCCAA ACAGAGACAC 
AGATCTATCA CTCCTGGCAC AATGCCGCAG AGCCCCTCCG ACTTTGCAGA TCAGCTGCTG 
AGCAGCTCAG AAAATGGAGG CTCCCTGAAA GATTCAGTAT CCAAAGATGA AAAGGAAGAG 
TCGGTGGAGG ATGCATTGGA AGGTCAGGAA GAGAAGGACT TCAGCGGCCT TACAGAATAC 
CTCCTTGCCA GCAACATTGA TCTGAACCAA GATGCAGCAT CTGGAGGAGG CAATGTTGCC 
AGCGAGGCCT TGGAAGAAGT GTCGGTGCTA AAACGCCTGC TAGTGCGCAA TATCCGCCTC 
AGTGTGGATG CAGACACGCT CTCAACCCTG GAGACCTGGT CAGCGGGGGA AGAACCTGCA 
CTGGAGGCTT CTGAACAGGG AAGGGCCTCC AATGGGGATC GAGAGGCAGA GGATTCTGAA 
ATGGAATCTA CACAACAGGG AGAGAATGGC AGAAAGCAGA GTTCTGCAGA TGTTGCAGAG 
TCCCTGTGCG CACCCATTGT ACTGTCCAAA GAACAGTCGG AGGAGGTGAA GAGGCACATT 
CTGTCACTGG AGCAGAGGAT CCAGCAGCTT AAGCTGATCG AGGAAGATTA CAACAACCTG 
CACAGAACAA TCGCCAAATT CACTGTACCG AGGAGCAGCT TTACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgef1 ( XM_012967442.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012822896.2 Xenopus tropicalis Rho guanine nucleotide exchange factor 1 (arhgef1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa45454
Clone ID Related Accession (Same CDS sequence)	XM_012967442.2
Accession Version	XM_012967442.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho guanine nucleotide exchange factor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012967442.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATCAT CCCCCTTGGC CATGACTCAC TCTGTCTACC TCTTACCCTC CTGCATGAGT 
TATTGTGGAT TCCAAAAGCA CGGCTCACAG GTTTCTCCTG GTCTGTGCAT CACTTATGCA 
TCAGCCGACC TGAGCAGGGG ATTCCCATTG TCTCACTCAC TGGGTTTTTG CCAGACTGCT 
CCCAAACCCC TCGTCTGCCA GATAAGAAGC ATGGATCCGG ACGATGGCCA TTTTGGGGGT 
TTGTACTCCA AGGGGTCCAT GTCGATCATT GGAGCAGAGG ATGAGGATTT TGAGAATGAC 
ATTGAACCGA ATATGGTTGA TGAGAGCAGT TACTTCCGAA GCATTGAGCT AGTGAAAGAA 
CACCCGGCCT ACATTATGGT GTTCTTGCAG CACGTGGTTC TTCAGTTTGA CCCCAGCCCA 
CTGCTCTGTT TCCTGCATGC GGAGATGCTA AAGAACCTTT CAGCCAAAGA GCTTAAGAAA 
GTCTTCATGG ATTTTCACTA CACATTTCTG ATGAAAGGAG CGGTACTGCA TGTCAGTGTG 
CCAAACAGCG TATCCTTTGA ACTGGATCGG ACAAGGCCTG AGATGATCCC AGAAGAAACC 
CAGAGAAAAT ATGTCAAAGA CATTCAGAAG CACCAAACTG AGGCCATTTC CCAACAGCTG 
GAGGACTTTA GGGCCAAGCG AGTACTTGGT ATGACCCCTG GGGAACTGGA GCTGAGCGAG 
CTTGAATCCT ATCGAACCAA CGACCGCAGT ATCCTGGAAG CCAAAGAGAA GCAGTTGGCA 
GAGCAGCTCC TGGCACGGCT GGATGAGATG CACCTAACTA TTGCTCCAGA CGAGGAAAAA 
AGCTCTGCCA TCTTTTGTGC CATTGTAGCC TACATGAAGC ACCTGGGGGT GAAGACCAAG 
GCGGCGGATA GCAAAAAATC TAAAGGAATC TTCTTCAGGA AAAAGGTTTC TACAAATAAA 
AAGGATGAGC CTCTGAAAAC AAAGAAAACA TTCAGCATCT TGGATCCTAT GCGCTGGAAC 
CGAAATGATT CTCACTATGT TGACAGCAGA CCATCCAAAG TTGAGCCCGA AGCTGAGAAG 
CCAAACCCGG ACAGAAGGGT CAGCCAGGGG CAGAGAACTT TTGATAGGAG TGACAGCAGC 
ACAGCGAAAT CCAAGAACAG CGAAGGGCCT GATTCTGTGG TGTCTGTAAA TATCATCCCC 
CCTCCTAATG AGCCCAATGA TCCTGACCCA GTGACTGATG GGGCATTGTC TAATGATGCA 
TCTGAGGTCC TCAAAGGATC CCCCACCACT GAGCATTGCC CAGCAGACCA GACTGAGGAA 
AGTGCAGAAA ATGAAAGACG AAAGAAAAGG CTCTCTGGTA AGCTGGGCCG CTCGGAGAGC 
CTACGAGTTT ACGAGCGCAA GCGTTCCCAG CGCGGCTCCT CCAAAGCCAA GCAGACACGT 
TCCCGTAGCG ACGTTGACCT GGAGGCAGCT GCCCGAGCCA ACGACTTGCA GGGCACCACT 
GGCAAGCTTG GGCCAAAGCC TGGCAACTAC ACCCCAGAGC CTGGAGGGAG TTCAGAGGGC 
CCGCTGCCCT TTCTGCTTCC CCAGTCTGAG GAGACTGAGC CGCGCGTTCA GGAGGCCGAG 
AGCGACCCCC CAAACTGGAG GGAGCTCGTC CCCCCTGAAA TACTCCTCAG GCTGAAGAAA 
AGCGAAATCA AGCGGCAAGA GGTCATCAAT GAGCTCTTCT TTACAGAGCA CGCTCATGTA 
CGGATGCTTC GTGTGTTGCA TGATGTCTTC TACATCCCCA TGCAGAATGG ATGGATCCCC 
ACCTTGGAGC TGGAGAACAT CTTTCCTAGC CTGGATGACC TTATCGAGGC TCATGCTGTA 
TTCCTGGACA CCTTGAAGCG TCTGCGCCTT GAGAATAACT CAGTGATCCA GGAAATAGGC 
ACCATCTTGC TGAACCGGTT TGACGGCACA GAGGGCAACT GGTTTCAGAA GATTTCATCT 
CGGTTCTGCA GCCGGCAGCC ATTTGCCTTG GAACAGTTGA AAGCGAAACA GAAGAGAGAC 
CCTCGATTTA CGCAGTTTAT TCAGGAAGCT GAGAGCAGCC CTCACTGCCG GCGGTTACAA 
CTAAAGGACA TTATACCATT TGAGATGCAA AGGCTGACCA AGTACCCGTT GCTGCTGCAG 
AACATCGCAA ATCTAACTGA GGAAGACGAG GAGAAGCAGA GAGTGCAGAG GGCAGCAGAG 
TCTTGCAGGC AGATCCTAAA CCATGTTAAC CAGGAGGTGC GCGAGATGGA GAATAAGATG 
AAACTTGCAG ATTACCAAAG ACGCCTGGAT GTTTCCAACC TGAGGCAAAT CAACGACCCC 
TTATTTAATG AATTTAAGAA CATTGATATC AGCAAAAAGA ATTTGATACA TGAAGGATCT 
TTGACATGGA AAGTGAGCAG GGACAAGGCT TTTGATGTCC ATGTCCTCCT CCTGGATGAT 
CTCTTGATGC TGCTGCTGAA ACAAGATGAG CGGCTGGTAC TGAAGTGCCA GAGTCGGACT 
ATTATTCCTG CTCCAGACGG GAAGCTCATG CTGAGCCCAA TCATCAAGCT GAATATGGCC 
ATGGTCAGAG AGGTGGCTAC AGATAAGAAT GCCTTTTTTG TGATTTTCAA CTGGGAGGAT 
CGAGCACAGT TCTATGAACT TGTGGCTCCA ACACCCGCTG AGAAGAAAAA CTGGTTTTTA 
CTGATCAGCA ACACTACAGG CTTATTGGAA CCCCCAGCTT CTTCCAAACA GAGACACAGA 
TCTATCACTC CTGGCACAAT GCCGCAGAGC CCCTCCGACT TTGCAGATCA GCTGCTGAGC 
AGCTCAGAAA ATGGAGGCTC CCTGAAAGAT TCAGTATCCA AAGATGAAAA GGAAGAGTCG 
GTGGAGGATG CATTGGAAGG TCAGGAAGAG AAGGACTTCA GCGGCCTTAC AGAATACCTC 
CTTGCCAGCA ACATTGATCT GAACCAAGAT GCAGCATCTG GAGGAGGCAA TGTTGCCAGC 
GAGGCCTTGG AAGAAGTGTC GGTGCTAAAA CGCCTGCTAG TGCGCAATAT CCGCCTCAGT 
GTGGATGCAG ACACGCTCTC AACCCTGGAG ACCTGGTCAG CGGGGGAAGA ACCTGCACTG 
GAGGCTTCTG AACAGGGAAG GGCCTCCAAT GGGGATCGAG AGGCAGAGGA TTCTGAAATG 
GAATCTACAC AACAGGGAGA GAATGGCAGA AAGCAGAGTT CTGCAGATGT TGCAGAGTCC 
CTGTGCGCAC CCATTGTACT GTCCAAAGAA CAGTCGGAGG AGGTGAAGAG GCACATTCTG 
TCACTGGAGC AGAGGATCCA GCAGCTTAAG CTGATCGAGG AAGATTACAA CAACCTGCAC 
AGAACAATCG CCAAATTCAC TGTACCGAGG AGCAGCTTTA CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012822896.2
CDS	26..3430
Translation	MESSPLAMTHSVYLLPSCMSYCGFQKHGSQVSPGLCITYASADLSRGFPLSHSLGFCQTAPKPLVCQIRSMDPDDGHFGGLYSKGSMSIIGAEDEDFENDIEPNMVDESSYFRSIELVKEHPAYIMVFLQHVVLQFDPSPLLCFLHAEMLKNLSAKELKKVFMDFHYTFLMKGAVLHVSVPNSVSFELDRTRPEMIPEETQRKYVKDIQKHQTEAISQQLEDFRAKRVLGMTPGELELSELESYRTNDRSILEAKEKQLAEQLLARLDEMHLTIAPDEEKSSAIFCAIVAYMKHLGVKTKAADSKKSKGIFFRKKVSTNKKDEPLKTKKTFSILDPMRWNRNDSHYVDSRPSKVEPEAEKPNPDRRVSQGQRTFDRSDSSTAKSKNSEGPDSVVSVNIIPPPNEPNDPDPVTDGALSNDASEVLKGSPTTEHCPADQTEESAENERRKKRLSGKLGRSESLRVYERKRSQRGSSKAKQTRSRSDVDLEAAARANDLQGTTGKLGPKPGNYTPEPGGSSEGPLPFLLPQSEETEPRVQEAESDPPNWRELVPPEILLRLKKSEIKRQEVINELFFTEHAHVRMLRVLHDVFYIPMQNGWIPTLELENIFPSLDDLIEAHAVFLDTLKRLRLENNSVIQEIGTILLNRFDGTEGNWFQKISSRFCSRQPFALEQLKAKQKRDPRFTQFIQEAESSPHCRRLQLKDIIPFEMQRLTKYPLLLQNIANLTEEDEEKQRVQRAAESCRQILNHVNQEVREMENKMKLADYQRRLDVSNLRQINDPLFNEFKNIDISKKNLIHEGSLTWKVSRDKAFDVHVLLLDDLLMLLLKQDERLVLKCQSRTIIPAPDGKLMLSPIIKLNMAMVREVATDKNAFFVIFNWEDRAQFYELVAPTPAEKKNWFLLISNTTGLLEPPASSKQRHRSITPGTMPQSPSDFADQLLSSSENGGSLKDSVSKDEKEESVEDALEGQEEKDFSGLTEYLLASNIDLNQDAASGGGNVASEALEEVSVLKRLLVRNIRLSVDADTLSTLETWSAGEEPALEASEQGRASNGDREAEDSEMESTQQGENGRKQSSADVAESLCAPIVLSKEQSEEVKRHILSLEQRIQQLKLIEEDYNNLHRTIAKFTVPRSSFT

Target ORF information:

RefSeq Version	XM_012967442.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho guanine nucleotide exchange factor 1 (arhgef1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012967442.2

ORF Insert Sequence:

ATGGAATCAT CCCCCTTGGC CATGACTCAC TCTGTCTACC TCTTACCCTC CTGCATGAGT 
TATTGTGGAT TCCAAAAGCA CGGCTCACAG GTTTCTCCTG GTCTGTGCAT CACTTATGCA 
TCAGCCGACC TGAGCAGGGG ATTCCCATTG TCTCACTCAC TGGGTTTTTG CCAGACTGCT 
CCCAAACCCC TCGTCTGCCA GATAAGAAGC ATGGATCCGG ACGATGGCCA TTTTGGGGGT 
TTGTACTCCA AGGGGTCCAT GTCGATCATT GGAGCAGAGG ATGAGGATTT TGAGAATGAC 
ATTGAACCGA ATATGGTTGA TGAGAGCAGT TACTTCCGAA GCATTGAGCT AGTGAAAGAA 
CACCCGGCCT ACATTATGGT GTTCTTGCAG CACGTGGTTC TTCAGTTTGA CCCCAGCCCA 
CTGCTCTGTT TCCTGCATGC GGAGATGCTA AAGAACCTTT CAGCCAAAGA GCTTAAGAAA 
GTCTTCATGG ATTTTCACTA CACATTTCTG ATGAAAGGAG CGGTACTGCA TGTCAGTGTG 
CCAAACAGCG TATCCTTTGA ACTGGATCGG ACAAGGCCTG AGATGATCCC AGAAGAAACC 
CAGAGAAAAT ATGTCAAAGA CATTCAGAAG CACCAAACTG AGGCCATTTC CCAACAGCTG 
GAGGACTTTA GGGCCAAGCG AGTACTTGGT ATGACCCCTG GGGAACTGGA GCTGAGCGAG 
CTTGAATCCT ATCGAACCAA CGACCGCAGT ATCCTGGAAG CCAAAGAGAA GCAGTTGGCA 
GAGCAGCTCC TGGCACGGCT GGATGAGATG CACCTAACTA TTGCTCCAGA CGAGGAAAAA 
AGCTCTGCCA TCTTTTGTGC CATTGTAGCC TACATGAAGC ACCTGGGGGT GAAGACCAAG 
GCGGCGGATA GCAAAAAATC TAAAGGAATC TTCTTCAGGA AAAAGGTTTC TACAAATAAA 
AAGGATGAGC CTCTGAAAAC AAAGAAAACA TTCAGCATCT TGGATCCTAT GCGCTGGAAC 
CGAAATGATT CTCACTATGT TGACAGCAGA CCATCCAAAG TTGAGCCCGA AGCTGAGAAG 
CCAAACCCGG ACAGAAGGGT CAGCCAGGGG CAGAGAACTT TTGATAGGAG TGACAGCAGC 
ACAGCGAAAT CCAAGAACAG CGAAGGGCCT GATTCTGTGG TGTCTGTAAA TATCATCCCC 
CCTCCTAATG AGCCCAATGA TCCTGACCCA GTGACTGATG GGGCATTGTC TAATGATGCA 
TCTGAGGTCC TCAAAGGATC CCCCACCACT GAGCATTGCC CAGCAGACCA GACTGAGGAA 
AGTGCAGAAA ATGAAAGACG AAAGAAAAGG CTCTCTGGTA AGCTGGGCCG CTCGGAGAGC 
CTACGAGTTT ACGAGCGCAA GCGTTCCCAG CGCGGCTCCT CCAAAGCCAA GCAGACACGT 
TCCCGTAGCG ACGTTGACCT GGAGGCAGCT GCCCGAGCCA ACGACTTGCA GGGCACCACT 
GGCAAGCTTG GGCCAAAGCC TGGCAACTAC ACCCCAGAGC CTGGAGGGAG TTCAGAGGGC 
CCGCTGCCCT TTCTGCTTCC CCAGTCTGAG GAGACTGAGC CGCGCGTTCA GGAGGCCGAG 
AGCGACCCCC CAAACTGGAG GGAGCTCGTC CCCCCTGAAA TACTCCTCAG GCTGAAGAAA 
AGCGAAATCA AGCGGCAAGA GGTCATCAAT GAGCTCTTCT TTACAGAGCA CGCTCATGTA 
CGGATGCTTC GTGTGTTGCA TGATGTCTTC TACATCCCCA TGCAGAATGG ATGGATCCCC 
ACCTTGGAGC TGGAGAACAT CTTTCCTAGC CTGGATGACC TTATCGAGGC TCATGCTGTA 
TTCCTGGACA CCTTGAAGCG TCTGCGCCTT GAGAATAACT CAGTGATCCA GGAAATAGGC 
ACCATCTTGC TGAACCGGTT TGACGGCACA GAGGGCAACT GGTTTCAGAA GATTTCATCT 
CGGTTCTGCA GCCGGCAGCC ATTTGCCTTG GAACAGTTGA AAGCGAAACA GAAGAGAGAC 
CCTCGATTTA CGCAGTTTAT TCAGGAAGCT GAGAGCAGCC CTCACTGCCG GCGGTTACAA 
CTAAAGGACA TTATACCATT TGAGATGCAA AGGCTGACCA AGTACCCGTT GCTGCTGCAG 
AACATCGCAA ATCTAACTGA GGAAGACGAG GAGAAGCAGA GAGTGCAGAG GGCAGCAGAG 
TCTTGCAGGC AGATCCTAAA CCATGTTAAC CAGGAGGTGC GCGAGATGGA GAATAAGATG 
AAACTTGCAG ATTACCAAAG ACGCCTGGAT GTTTCCAACC TGAGGCAAAT CAACGACCCC 
TTATTTAATG AATTTAAGAA CATTGATATC AGCAAAAAGA ATTTGATACA TGAAGGATCT 
TTGACATGGA AAGTGAGCAG GGACAAGGCT TTTGATGTCC ATGTCCTCCT CCTGGATGAT 
CTCTTGATGC TGCTGCTGAA ACAAGATGAG CGGCTGGTAC TGAAGTGCCA GAGTCGGACT 
ATTATTCCTG CTCCAGACGG GAAGCTCATG CTGAGCCCAA TCATCAAGCT GAATATGGCC 
ATGGTCAGAG AGGTGGCTAC AGATAAGAAT GCCTTTTTTG TGATTTTCAA CTGGGAGGAT 
CGAGCACAGT TCTATGAACT TGTGGCTCCA ACACCCGCTG AGAAGAAAAA CTGGTTTTTA 
CTGATCAGCA ACACTACAGG CTTATTGGAA CCCCCAGCTT CTTCCAAACA GAGACACAGA 
TCTATCACTC CTGGCACAAT GCCGCAGAGC CCCTCCGACT TTGCAGATCA GCTGCTGAGC 
AGCTCAGAAA ATGGAGGCTC CCTGAAAGAT TCAGTATCCA AAGATGAAAA GGAAGAGTCG 
GTGGAGGATG CATTGGAAGG TCAGGAAGAG AAGGACTTCA GCGGCCTTAC AGAATACCTC 
CTTGCCAGCA ACATTGATCT GAACCAAGAT GCAGCATCTG GAGGAGGCAA TGTTGCCAGC 
GAGGCCTTGG AAGAAGTGTC GGTGCTAAAA CGCCTGCTAG TGCGCAATAT CCGCCTCAGT 
GTGGATGCAG ACACGCTCTC AACCCTGGAG ACCTGGTCAG CGGGGGAAGA ACCTGCACTG 
GAGGCTTCTG AACAGGGAAG GGCCTCCAAT GGGGATCGAG AGGCAGAGGA TTCTGAAATG 
GAATCTACAC AACAGGGAGA GAATGGCAGA AAGCAGAGTT CTGCAGATGT TGCAGAGTCC 
CTGTGCGCAC CCATTGTACT GTCCAAAGAA CAGTCGGAGG AGGTGAAGAG GCACATTCTG 
TCACTGGAGC AGAGGATCCA GCAGCTTAAG CTGATCGAGG AAGATTACAA CAACCTGCAC 
AGAACAATCG CCAAATTCAC TGTACCGAGG AGCAGCTTTA CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgef1 ( XM_012967444.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012822898.2 Xenopus tropicalis Rho guanine nucleotide exchange factor 1 (arhgef1), transcript variant X4, mRNA.

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CloneID	OXa45455
Clone ID Related Accession (Same CDS sequence)	XM_012967444.2
Accession Version	XM_012967444.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3372bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho guanine nucleotide exchange factor 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012967444.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATCAT CCCCCTTGGC CATGACTCAC TCTGTCTACC TCTTACCCTC CTGCATGAGT 
TATTGTGGAT TCCAAAAGCA CGGCTCACAG GTTTCTCCTG GTCTGTGCAT CACTTATGCA 
TCAGCCGACC TGAGCAGGGG ATTCCCATTG TCTCACTCAC TGGGTTTTTG CCAGACTGCT 
CCCAAACCCC TCGTCTGCCA GATAAGAAGC ATGGATCCGG ACGATGGCCA TTTTGGGGGT 
TTGTACTCCA AGGGGTCCAT GTCGATCATT GGAGCAGAGG ATGAGGATTT TGAGAATGAC 
ATTGAACCGA ATATGGTTGA TGAGAGCAGT TACTTCCGAA GCATTGAGCT AGTGAAAGAA 
CACCCGGCCT ACATTATGGT GTTCTTGCAG CACGTGGTTC TTCAGTTTGA CCCCAGCCCA 
CTGCTCTGTT TCCTGCATGC GGAGATGCTA AAGAACCTTT CAGCCAAAGA GCTTAAGAAA 
GTCTTCATGG ATTTTCACTA CACATTTCTG ATGAAAGGAG CGGTACTGCA TGTCAGTGTG 
CCAAACAGCG TATCCTTTGA ACTGGATCGG ACAAGGCCTG AGATGATCCC AGAAGAAACC 
CAGAGAAAAT ATGTCAAAGA CATTCAGAAG CACCAAACTG AGGCCATTTC CCAACAGCTG 
GAGGACTTTA GGGCCAAGCG AGTACTTGGT ATGACCCCTG GGGAACTGGA GCTGAGCGAG 
CTTGAATCCT ATCGAACCAA CGACCGCAGT ATCCTGGAAG CCAAAGAGAA GCAGTTGGCA 
GAGCAGCTCC TGGCACGGCT GGATGAGATG CACCTAACTA TTGCTCCAGA CGAGGAAAAA 
AGCTCTGCCA TCTTTTGTGC CATTGTAGCC TACATGAAGC ACCTGGGGGT GAAGACCAAG 
GCGGCGGATA GCAAAAAATC TAAAGGAATC TTCTTCAGGA AAAAGGTTTC TACAAATAAA 
AAGGATGAGC CTCTGAAAAC AAAGAAAACA TTCAGCATCT TGGATCCTAT GCGCTGGAAC 
CGAAATGATT CTCACTCTGA GAAGCCAAAC CCGGACAGAA GGGTCAGCCA GGGGCAGAGA 
ACTTTTGATA GGAGTGACAG CAGCACAGCG AAATCCAAGA ACAGCGAAGG GCCTGATTCT 
GTGGTGTCTG TAAATATCAT CCCCCCTCCT AATGAGCCCA ATGATCCTGA CCCAGCAGTG 
ACTGATGGGG CATTGTCTAA TGATGCATCT GAGGTCCTCA AAGGATCCCC CACCACTGAG 
CATTGCCCAG CAGACCAGAC TGAGGAAAGT GCAGAAAATG AAAGACGAAA GAAAAGGCTC 
TCTGGTAAGC TGGGCCGCTC GGAGAGCCTA CGAGTTTACG AGCGCAAGCG TTCCCAGCGC 
GGCTCCTCCA AAGCCAAGCA GACACGTTCC CGTAGCGACG TTGACCTGGA GGCAGCTGCC 
CGAGCCAACG ACTTGCAGGG CACCACTGGC AAGCTTGGGC CAAAGCCTGG CAACTACACC 
CCAGAGCCTG GAGGGAGTTC AGAGGGCCCG CTGCCCTTTC TGCTTCCCCA GTCTGAGGAG 
ACTGAGCCGC GCGTTCAGGA GGCCGAGAGC GACCCCCCAA ACTGGAGGGA GCTCGTCCCC 
CCTGAAATAC TCCTCAGGCT GAAGAAAAGC GAAATCAAGC GGCAAGAGGT CATCAATGAG 
CTCTTCTTTA CAGAGCACGC TCATGTACGG ATGCTTCGTG TGTTGCATGA TGTCTTCTAC 
ATCCCCATGC AGAATGGATG GATCCCCACC TTGGAGCTGG AGAACATCTT TCCTAGCCTG 
GATGACCTTA TCGAGGCTCA TGCTGTATTC CTGGACACCT TGAAGCGTCT GCGCCTTGAG 
AATAACTCAG TGATCCAGGA AATAGGCACC ATCTTGCTGA ACCGGTTTGA CGGCACAGAG 
GGCAACTGGT TTCAGAAGAT TTCATCTCGG TTCTGCAGCC GGCAGCCATT TGCCTTGGAA 
CAGTTGAAAG CGAAACAGAA GAGAGACCCT CGATTTACGC AGTTTATTCA GGAAGCTGAG 
AGCAGCCCTC ACTGCCGGCG GTTACAACTA AAGGACATTA TACCATTTGA GATGCAAAGG 
CTGACCAAGT ACCCGTTGCT GCTGCAGAAC ATCGCAAATC TAACTGAGGA AGACGAGGAG 
AAGCAGAGAG TGCAGAGGGC AGCAGAGTCT TGCAGGCAGA TCCTAAACCA TGTTAACCAG 
GAGGTGCGCG AGATGGAGAA TAAGATGAAA CTTGCAGATT ACCAAAGACG CCTGGATGTT 
TCCAACCTGA GGCAAATCAA CGACCCCTTA TTTAATGAAT TTAAGAACAT TGATATCAGC 
AAAAAGAATT TGATACATGA AGGATCTTTG ACATGGAAAG TGAGCAGGGA CAAGGCTTTT 
GATGTCCATG TCCTCCTCCT GGATGATCTC TTGATGCTGC TGCTGAAACA AGATGAGCGG 
CTGGTACTGA AGTGCCAGAG TCGGACTATT ATTCCTGCTC CAGACGGGAA GCTCATGCTG 
AGCCCAATCA TCAAGCTGAA TATGGCCATG GTCAGAGAGG TGGCTACAGA TAAGAATGCC 
TTTTTTGTGA TTTTCAACTG GGAGGATCGA GCACAGTTCT ATGAACTTGT GGCTCCAACA 
CCCGCTGAGA AGAAAAACTG GTTTTTACTG ATCAGCAACA CTACAGGCTT ATTGGAACCC 
CCAGCTTCTT CCAAACAGAG ACACAGATCT ATCACTCCTG GCACAATGCC GCAGAGCCCC 
TCCGACTTTG CAGATCAGCT GCTGAGCAGC TCAGAAAATG GAGGCTCCCT GAAAGATTCA 
GTATCCAAAG ATGAAAAGGA AGAGTCGGTG GAGGATGCAT TGGAAGGTCA GGAAGAGAAG 
GACTTCAGCG GCCTTACAGA ATACCTCCTT GCCAGCAACA TTGATCTGAA CCAAGATGCA 
GCATCTGGAG GAGGCAATGT TGCCAGCGAG GCCTTGGAAG AAGTGTCGGT GCTAAAACGC 
CTGCTAGTGC GCAATATCCG CCTCAGTGTG GATGCAGACA CGCTCTCAAC CCTGGAGACC 
TGGTCAGCGG GGGAAGAACC TGCACTGGAG GCTTCTGAAC AGGGAAGGGC CTCCAATGGG 
GATCGAGAGG CAGAGGATTC TGAAATGGAA TCTACACAAC AGGGAGAGAA TGGCAGAAAG 
CAGAGTTCTG CAGATGTTGC AGAGTCCCTG TGCGCACCCA TTGTACTGTC CAAAGAACAG 
TCGGAGGAGG TGAAGAGGCA CATTCTGTCA CTGGAGCAGA GGATCCAGCA GCTTAAGCTG 
ATCGAGGAAG ATTACAACAA CCTGCACAGA ACAATCGCCA AATTCACTGT ACCGAGGAGC 
AGCTTTACAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012822898.2
CDS	20..3391
Translation	MESSPLAMTHSVYLLPSCMSYCGFQKHGSQVSPGLCITYASADLSRGFPLSHSLGFCQTAPKPLVCQIRSMDPDDGHFGGLYSKGSMSIIGAEDEDFENDIEPNMVDESSYFRSIELVKEHPAYIMVFLQHVVLQFDPSPLLCFLHAEMLKNLSAKELKKVFMDFHYTFLMKGAVLHVSVPNSVSFELDRTRPEMIPEETQRKYVKDIQKHQTEAISQQLEDFRAKRVLGMTPGELELSELESYRTNDRSILEAKEKQLAEQLLARLDEMHLTIAPDEEKSSAIFCAIVAYMKHLGVKTKAADSKKSKGIFFRKKVSTNKKDEPLKTKKTFSILDPMRWNRNDSHSEKPNPDRRVSQGQRTFDRSDSSTAKSKNSEGPDSVVSVNIIPPPNEPNDPDPAVTDGALSNDASEVLKGSPTTEHCPADQTEESAENERRKKRLSGKLGRSESLRVYERKRSQRGSSKAKQTRSRSDVDLEAAARANDLQGTTGKLGPKPGNYTPEPGGSSEGPLPFLLPQSEETEPRVQEAESDPPNWRELVPPEILLRLKKSEIKRQEVINELFFTEHAHVRMLRVLHDVFYIPMQNGWIPTLELENIFPSLDDLIEAHAVFLDTLKRLRLENNSVIQEIGTILLNRFDGTEGNWFQKISSRFCSRQPFALEQLKAKQKRDPRFTQFIQEAESSPHCRRLQLKDIIPFEMQRLTKYPLLLQNIANLTEEDEEKQRVQRAAESCRQILNHVNQEVREMENKMKLADYQRRLDVSNLRQINDPLFNEFKNIDISKKNLIHEGSLTWKVSRDKAFDVHVLLLDDLLMLLLKQDERLVLKCQSRTIIPAPDGKLMLSPIIKLNMAMVREVATDKNAFFVIFNWEDRAQFYELVAPTPAEKKNWFLLISNTTGLLEPPASSKQRHRSITPGTMPQSPSDFADQLLSSSENGGSLKDSVSKDEKEESVEDALEGQEEKDFSGLTEYLLASNIDLNQDAASGGGNVASEALEEVSVLKRLLVRNIRLSVDADTLSTLETWSAGEEPALEASEQGRASNGDREAEDSEMESTQQGENGRKQSSADVAESLCAPIVLSKEQSEEVKRHILSLEQRIQQLKLIEEDYNNLHRTIAKFTVPRSSFT

Target ORF information:

RefSeq Version	XM_012967444.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho guanine nucleotide exchange factor 1 (arhgef1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012967444.2

ORF Insert Sequence:

ATGGAATCAT CCCCCTTGGC CATGACTCAC TCTGTCTACC TCTTACCCTC CTGCATGAGT 
TATTGTGGAT TCCAAAAGCA CGGCTCACAG GTTTCTCCTG GTCTGTGCAT CACTTATGCA 
TCAGCCGACC TGAGCAGGGG ATTCCCATTG TCTCACTCAC TGGGTTTTTG CCAGACTGCT 
CCCAAACCCC TCGTCTGCCA GATAAGAAGC ATGGATCCGG ACGATGGCCA TTTTGGGGGT 
TTGTACTCCA AGGGGTCCAT GTCGATCATT GGAGCAGAGG ATGAGGATTT TGAGAATGAC 
ATTGAACCGA ATATGGTTGA TGAGAGCAGT TACTTCCGAA GCATTGAGCT AGTGAAAGAA 
CACCCGGCCT ACATTATGGT GTTCTTGCAG CACGTGGTTC TTCAGTTTGA CCCCAGCCCA 
CTGCTCTGTT TCCTGCATGC GGAGATGCTA AAGAACCTTT CAGCCAAAGA GCTTAAGAAA 
GTCTTCATGG ATTTTCACTA CACATTTCTG ATGAAAGGAG CGGTACTGCA TGTCAGTGTG 
CCAAACAGCG TATCCTTTGA ACTGGATCGG ACAAGGCCTG AGATGATCCC AGAAGAAACC 
CAGAGAAAAT ATGTCAAAGA CATTCAGAAG CACCAAACTG AGGCCATTTC CCAACAGCTG 
GAGGACTTTA GGGCCAAGCG AGTACTTGGT ATGACCCCTG GGGAACTGGA GCTGAGCGAG 
CTTGAATCCT ATCGAACCAA CGACCGCAGT ATCCTGGAAG CCAAAGAGAA GCAGTTGGCA 
GAGCAGCTCC TGGCACGGCT GGATGAGATG CACCTAACTA TTGCTCCAGA CGAGGAAAAA 
AGCTCTGCCA TCTTTTGTGC CATTGTAGCC TACATGAAGC ACCTGGGGGT GAAGACCAAG 
GCGGCGGATA GCAAAAAATC TAAAGGAATC TTCTTCAGGA AAAAGGTTTC TACAAATAAA 
AAGGATGAGC CTCTGAAAAC AAAGAAAACA TTCAGCATCT TGGATCCTAT GCGCTGGAAC 
CGAAATGATT CTCACTCTGA GAAGCCAAAC CCGGACAGAA GGGTCAGCCA GGGGCAGAGA 
ACTTTTGATA GGAGTGACAG CAGCACAGCG AAATCCAAGA ACAGCGAAGG GCCTGATTCT 
GTGGTGTCTG TAAATATCAT CCCCCCTCCT AATGAGCCCA ATGATCCTGA CCCAGCAGTG 
ACTGATGGGG CATTGTCTAA TGATGCATCT GAGGTCCTCA AAGGATCCCC CACCACTGAG 
CATTGCCCAG CAGACCAGAC TGAGGAAAGT GCAGAAAATG AAAGACGAAA GAAAAGGCTC 
TCTGGTAAGC TGGGCCGCTC GGAGAGCCTA CGAGTTTACG AGCGCAAGCG TTCCCAGCGC 
GGCTCCTCCA AAGCCAAGCA GACACGTTCC CGTAGCGACG TTGACCTGGA GGCAGCTGCC 
CGAGCCAACG ACTTGCAGGG CACCACTGGC AAGCTTGGGC CAAAGCCTGG CAACTACACC 
CCAGAGCCTG GAGGGAGTTC AGAGGGCCCG CTGCCCTTTC TGCTTCCCCA GTCTGAGGAG 
ACTGAGCCGC GCGTTCAGGA GGCCGAGAGC GACCCCCCAA ACTGGAGGGA GCTCGTCCCC 
CCTGAAATAC TCCTCAGGCT GAAGAAAAGC GAAATCAAGC GGCAAGAGGT CATCAATGAG 
CTCTTCTTTA CAGAGCACGC TCATGTACGG ATGCTTCGTG TGTTGCATGA TGTCTTCTAC 
ATCCCCATGC AGAATGGATG GATCCCCACC TTGGAGCTGG AGAACATCTT TCCTAGCCTG 
GATGACCTTA TCGAGGCTCA TGCTGTATTC CTGGACACCT TGAAGCGTCT GCGCCTTGAG 
AATAACTCAG TGATCCAGGA AATAGGCACC ATCTTGCTGA ACCGGTTTGA CGGCACAGAG 
GGCAACTGGT TTCAGAAGAT TTCATCTCGG TTCTGCAGCC GGCAGCCATT TGCCTTGGAA 
CAGTTGAAAG CGAAACAGAA GAGAGACCCT CGATTTACGC AGTTTATTCA GGAAGCTGAG 
AGCAGCCCTC ACTGCCGGCG GTTACAACTA AAGGACATTA TACCATTTGA GATGCAAAGG 
CTGACCAAGT ACCCGTTGCT GCTGCAGAAC ATCGCAAATC TAACTGAGGA AGACGAGGAG 
AAGCAGAGAG TGCAGAGGGC AGCAGAGTCT TGCAGGCAGA TCCTAAACCA TGTTAACCAG 
GAGGTGCGCG AGATGGAGAA TAAGATGAAA CTTGCAGATT ACCAAAGACG CCTGGATGTT 
TCCAACCTGA GGCAAATCAA CGACCCCTTA TTTAATGAAT TTAAGAACAT TGATATCAGC 
AAAAAGAATT TGATACATGA AGGATCTTTG ACATGGAAAG TGAGCAGGGA CAAGGCTTTT 
GATGTCCATG TCCTCCTCCT GGATGATCTC TTGATGCTGC TGCTGAAACA AGATGAGCGG 
CTGGTACTGA AGTGCCAGAG TCGGACTATT ATTCCTGCTC CAGACGGGAA GCTCATGCTG 
AGCCCAATCA TCAAGCTGAA TATGGCCATG GTCAGAGAGG TGGCTACAGA TAAGAATGCC 
TTTTTTGTGA TTTTCAACTG GGAGGATCGA GCACAGTTCT ATGAACTTGT GGCTCCAACA 
CCCGCTGAGA AGAAAAACTG GTTTTTACTG ATCAGCAACA CTACAGGCTT ATTGGAACCC 
CCAGCTTCTT CCAAACAGAG ACACAGATCT ATCACTCCTG GCACAATGCC GCAGAGCCCC 
TCCGACTTTG CAGATCAGCT GCTGAGCAGC TCAGAAAATG GAGGCTCCCT GAAAGATTCA 
GTATCCAAAG ATGAAAAGGA AGAGTCGGTG GAGGATGCAT TGGAAGGTCA GGAAGAGAAG 
GACTTCAGCG GCCTTACAGA ATACCTCCTT GCCAGCAACA TTGATCTGAA CCAAGATGCA 
GCATCTGGAG GAGGCAATGT TGCCAGCGAG GCCTTGGAAG AAGTGTCGGT GCTAAAACGC 
CTGCTAGTGC GCAATATCCG CCTCAGTGTG GATGCAGACA CGCTCTCAAC CCTGGAGACC 
TGGTCAGCGG GGGAAGAACC TGCACTGGAG GCTTCTGAAC AGGGAAGGGC CTCCAATGGG 
GATCGAGAGG CAGAGGATTC TGAAATGGAA TCTACACAAC AGGGAGAGAA TGGCAGAAAG 
CAGAGTTCTG CAGATGTTGC AGAGTCCCTG TGCGCACCCA TTGTACTGTC CAAAGAACAG 
TCGGAGGAGG TGAAGAGGCA CATTCTGTCA CTGGAGCAGA GGATCCAGCA GCTTAAGCTG 
ATCGAGGAAG ATTACAACAA CCTGCACAGA ACAATCGCCA AATTCACTGT ACCGAGGAGC 
AGCTTTACAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgef1 ( XM_012967445.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012822899.2 Xenopus tropicalis Rho guanine nucleotide exchange factor 1 (arhgef1), transcript variant X5, mRNA.

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CloneID	OXa45456
Clone ID Related Accession (Same CDS sequence)	XM_012967445.2
Accession Version	XM_012967445.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3369bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho guanine nucleotide exchange factor 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012967445.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATCAT CCCCCTTGGC CATGACTCAC TCTGTCTACC TCTTACCCTC CTGCATGAGT 
TATTGTGGAT TCCAAAAGCA CGGCTCACAG GTTTCTCCTG GTCTGTGCAT CACTTATGCA 
TCAGCCGACC TGAGCAGGGG ATTCCCATTG TCTCACTCAC TGGGTTTTTG CCAGACTGCT 
CCCAAACCCC TCGTCTGCCA GATAAGAAGC ATGGATCCGG ACGATGGCCA TTTTGGGGGT 
TTGTACTCCA AGGGGTCCAT GTCGATCATT GGAGCAGAGG ATGAGGATTT TGAGAATGAC 
ATTGAACCGA ATATGGTTGA TGAGAGCAGT TACTTCCGAA GCATTGAGCT AGTGAAAGAA 
CACCCGGCCT ACATTATGGT GTTCTTGCAG CACGTGGTTC TTCAGTTTGA CCCCAGCCCA 
CTGCTCTGTT TCCTGCATGC GGAGATGCTA AAGAACCTTT CAGCCAAAGA GCTTAAGAAA 
GTCTTCATGG ATTTTCACTA CACATTTCTG ATGAAAGGAG CGGTACTGCA TGTCAGTGTG 
CCAAACAGCG TATCCTTTGA ACTGGATCGG ACAAGGCCTG AGATGATCCC AGAAGAAACC 
CAGAGAAAAT ATGTCAAAGA CATTCAGAAG CACCAAACTG AGGCCATTTC CCAACAGCTG 
GAGGACTTTA GGGCCAAGCG AGTACTTGGT ATGACCCCTG GGGAACTGGA GCTGAGCGAG 
CTTGAATCCT ATCGAACCAA CGACCGCAGT ATCCTGGAAG CCAAAGAGAA GCAGTTGGCA 
GAGCAGCTCC TGGCACGGCT GGATGAGATG CACCTAACTA TTGCTCCAGA CGAGGAAAAA 
AGCTCTGCCA TCTTTTGTGC CATTGTAGCC TACATGAAGC ACCTGGGGGT GAAGACCAAG 
GCGGCGGATA GCAAAAAATC TAAAGGAATC TTCTTCAGGA AAAAGGTTTC TACAAATAAA 
AAGGATGAGC CTCTGAAAAC AAAGAAAACA TTCAGCATCT TGGATCCTAT GCGCTGGAAC 
CGAAATGATT CTCACTCTGA GAAGCCAAAC CCGGACAGAA GGGTCAGCCA GGGGCAGAGA 
ACTTTTGATA GGAGTGACAG CAGCACAGCG AAATCCAAGA ACAGCGAAGG GCCTGATTCT 
GTGGTGTCTG TAAATATCAT CCCCCCTCCT AATGAGCCCA ATGATCCTGA CCCAGTGACT 
GATGGGGCAT TGTCTAATGA TGCATCTGAG GTCCTCAAAG GATCCCCCAC CACTGAGCAT 
TGCCCAGCAG ACCAGACTGA GGAAAGTGCA GAAAATGAAA GACGAAAGAA AAGGCTCTCT 
GGTAAGCTGG GCCGCTCGGA GAGCCTACGA GTTTACGAGC GCAAGCGTTC CCAGCGCGGC 
TCCTCCAAAG CCAAGCAGAC ACGTTCCCGT AGCGACGTTG ACCTGGAGGC AGCTGCCCGA 
GCCAACGACT TGCAGGGCAC CACTGGCAAG CTTGGGCCAA AGCCTGGCAA CTACACCCCA 
GAGCCTGGAG GGAGTTCAGA GGGCCCGCTG CCCTTTCTGC TTCCCCAGTC TGAGGAGACT 
GAGCCGCGCG TTCAGGAGGC CGAGAGCGAC CCCCCAAACT GGAGGGAGCT CGTCCCCCCT 
GAAATACTCC TCAGGCTGAA GAAAAGCGAA ATCAAGCGGC AAGAGGTCAT CAATGAGCTC 
TTCTTTACAG AGCACGCTCA TGTACGGATG CTTCGTGTGT TGCATGATGT CTTCTACATC 
CCCATGCAGA ATGGATGGAT CCCCACCTTG GAGCTGGAGA ACATCTTTCC TAGCCTGGAT 
GACCTTATCG AGGCTCATGC TGTATTCCTG GACACCTTGA AGCGTCTGCG CCTTGAGAAT 
AACTCAGTGA TCCAGGAAAT AGGCACCATC TTGCTGAACC GGTTTGACGG CACAGAGGGC 
AACTGGTTTC AGAAGATTTC ATCTCGGTTC TGCAGCCGGC AGCCATTTGC CTTGGAACAG 
TTGAAAGCGA AACAGAAGAG AGACCCTCGA TTTACGCAGT TTATTCAGGA AGCTGAGAGC 
AGCCCTCACT GCCGGCGGTT ACAACTAAAG GACATTATAC CATTTGAGAT GCAAAGGCTG 
ACCAAGTACC CGTTGCTGCT GCAGAACATC GCAAATCTAA CTGAGGAAGA CGAGGAGAAG 
CAGAGAGTGC AGAGGGCAGC AGAGTCTTGC AGGCAGATCC TAAACCATGT TAACCAGGAG 
GTGCGCGAGA TGGAGAATAA GATGAAACTT GCAGATTACC AAAGACGCCT GGATGTTTCC 
AACCTGAGGC AAATCAACGA CCCCTTATTT AATGAATTTA AGAACATTGA TATCAGCAAA 
AAGAATTTGA TACATGAAGG ATCTTTGACA TGGAAAGTGA GCAGGGACAA GGCTTTTGAT 
GTCCATGTCC TCCTCCTGGA TGATCTCTTG ATGCTGCTGC TGAAACAAGA TGAGCGGCTG 
GTACTGAAGT GCCAGAGTCG GACTATTATT CCTGCTCCAG ACGGGAAGCT CATGCTGAGC 
CCAATCATCA AGCTGAATAT GGCCATGGTC AGAGAGGTGG CTACAGATAA GAATGCCTTT 
TTTGTGATTT TCAACTGGGA GGATCGAGCA CAGTTCTATG AACTTGTGGC TCCAACACCC 
GCTGAGAAGA AAAACTGGTT TTTACTGATC AGCAACACTA CAGGCTTATT GGAACCCCCA 
GCTTCTTCCA AACAGAGACA CAGATCTATC ACTCCTGGCA CAATGCCGCA GAGCCCCTCC 
GACTTTGCAG ATCAGCTGCT GAGCAGCTCA GAAAATGGAG GCTCCCTGAA AGATTCAGTA 
TCCAAAGATG AAAAGGAAGA GTCGGTGGAG GATGCATTGG AAGGTCAGGA AGAGAAGGAC 
TTCAGCGGCC TTACAGAATA CCTCCTTGCC AGCAACATTG ATCTGAACCA AGATGCAGCA 
TCTGGAGGAG GCAATGTTGC CAGCGAGGCC TTGGAAGAAG TGTCGGTGCT AAAACGCCTG 
CTAGTGCGCA ATATCCGCCT CAGTGTGGAT GCAGACACGC TCTCAACCCT GGAGACCTGG 
TCAGCGGGGG AAGAACCTGC ACTGGAGGCT TCTGAACAGG GAAGGGCCTC CAATGGGGAT 
CGAGAGGCAG AGGATTCTGA AATGGAATCT ACACAACAGG GAGAGAATGG CAGAAAGCAG 
AGTTCTGCAG ATGTTGCAGA GTCCCTGTGC GCACCCATTG TACTGTCCAA AGAACAGTCG 
GAGGAGGTGA AGAGGCACAT TCTGTCACTG GAGCAGAGGA TCCAGCAGCT TAAGCTGATC 
GAGGAAGATT ACAACAACCT GCACAGAACA ATCGCCAAAT TCACTGTACC GAGGAGCAGC 
TTTACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012822899.2
CDS	28..3396
Translation	MESSPLAMTHSVYLLPSCMSYCGFQKHGSQVSPGLCITYASADLSRGFPLSHSLGFCQTAPKPLVCQIRSMDPDDGHFGGLYSKGSMSIIGAEDEDFENDIEPNMVDESSYFRSIELVKEHPAYIMVFLQHVVLQFDPSPLLCFLHAEMLKNLSAKELKKVFMDFHYTFLMKGAVLHVSVPNSVSFELDRTRPEMIPEETQRKYVKDIQKHQTEAISQQLEDFRAKRVLGMTPGELELSELESYRTNDRSILEAKEKQLAEQLLARLDEMHLTIAPDEEKSSAIFCAIVAYMKHLGVKTKAADSKKSKGIFFRKKVSTNKKDEPLKTKKTFSILDPMRWNRNDSHSEKPNPDRRVSQGQRTFDRSDSSTAKSKNSEGPDSVVSVNIIPPPNEPNDPDPVTDGALSNDASEVLKGSPTTEHCPADQTEESAENERRKKRLSGKLGRSESLRVYERKRSQRGSSKAKQTRSRSDVDLEAAARANDLQGTTGKLGPKPGNYTPEPGGSSEGPLPFLLPQSEETEPRVQEAESDPPNWRELVPPEILLRLKKSEIKRQEVINELFFTEHAHVRMLRVLHDVFYIPMQNGWIPTLELENIFPSLDDLIEAHAVFLDTLKRLRLENNSVIQEIGTILLNRFDGTEGNWFQKISSRFCSRQPFALEQLKAKQKRDPRFTQFIQEAESSPHCRRLQLKDIIPFEMQRLTKYPLLLQNIANLTEEDEEKQRVQRAAESCRQILNHVNQEVREMENKMKLADYQRRLDVSNLRQINDPLFNEFKNIDISKKNLIHEGSLTWKVSRDKAFDVHVLLLDDLLMLLLKQDERLVLKCQSRTIIPAPDGKLMLSPIIKLNMAMVREVATDKNAFFVIFNWEDRAQFYELVAPTPAEKKNWFLLISNTTGLLEPPASSKQRHRSITPGTMPQSPSDFADQLLSSSENGGSLKDSVSKDEKEESVEDALEGQEEKDFSGLTEYLLASNIDLNQDAASGGGNVASEALEEVSVLKRLLVRNIRLSVDADTLSTLETWSAGEEPALEASEQGRASNGDREAEDSEMESTQQGENGRKQSSADVAESLCAPIVLSKEQSEEVKRHILSLEQRIQQLKLIEEDYNNLHRTIAKFTVPRSSFT

Target ORF information:

RefSeq Version	XM_012967445.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho guanine nucleotide exchange factor 1 (arhgef1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012967445.2

ORF Insert Sequence:

ATGGAATCAT CCCCCTTGGC CATGACTCAC TCTGTCTACC TCTTACCCTC CTGCATGAGT 
TATTGTGGAT TCCAAAAGCA CGGCTCACAG GTTTCTCCTG GTCTGTGCAT CACTTATGCA 
TCAGCCGACC TGAGCAGGGG ATTCCCATTG TCTCACTCAC TGGGTTTTTG CCAGACTGCT 
CCCAAACCCC TCGTCTGCCA GATAAGAAGC ATGGATCCGG ACGATGGCCA TTTTGGGGGT 
TTGTACTCCA AGGGGTCCAT GTCGATCATT GGAGCAGAGG ATGAGGATTT TGAGAATGAC 
ATTGAACCGA ATATGGTTGA TGAGAGCAGT TACTTCCGAA GCATTGAGCT AGTGAAAGAA 
CACCCGGCCT ACATTATGGT GTTCTTGCAG CACGTGGTTC TTCAGTTTGA CCCCAGCCCA 
CTGCTCTGTT TCCTGCATGC GGAGATGCTA AAGAACCTTT CAGCCAAAGA GCTTAAGAAA 
GTCTTCATGG ATTTTCACTA CACATTTCTG ATGAAAGGAG CGGTACTGCA TGTCAGTGTG 
CCAAACAGCG TATCCTTTGA ACTGGATCGG ACAAGGCCTG AGATGATCCC AGAAGAAACC 
CAGAGAAAAT ATGTCAAAGA CATTCAGAAG CACCAAACTG AGGCCATTTC CCAACAGCTG 
GAGGACTTTA GGGCCAAGCG AGTACTTGGT ATGACCCCTG GGGAACTGGA GCTGAGCGAG 
CTTGAATCCT ATCGAACCAA CGACCGCAGT ATCCTGGAAG CCAAAGAGAA GCAGTTGGCA 
GAGCAGCTCC TGGCACGGCT GGATGAGATG CACCTAACTA TTGCTCCAGA CGAGGAAAAA 
AGCTCTGCCA TCTTTTGTGC CATTGTAGCC TACATGAAGC ACCTGGGGGT GAAGACCAAG 
GCGGCGGATA GCAAAAAATC TAAAGGAATC TTCTTCAGGA AAAAGGTTTC TACAAATAAA 
AAGGATGAGC CTCTGAAAAC AAAGAAAACA TTCAGCATCT TGGATCCTAT GCGCTGGAAC 
CGAAATGATT CTCACTCTGA GAAGCCAAAC CCGGACAGAA GGGTCAGCCA GGGGCAGAGA 
ACTTTTGATA GGAGTGACAG CAGCACAGCG AAATCCAAGA ACAGCGAAGG GCCTGATTCT 
GTGGTGTCTG TAAATATCAT CCCCCCTCCT AATGAGCCCA ATGATCCTGA CCCAGTGACT 
GATGGGGCAT TGTCTAATGA TGCATCTGAG GTCCTCAAAG GATCCCCCAC CACTGAGCAT 
TGCCCAGCAG ACCAGACTGA GGAAAGTGCA GAAAATGAAA GACGAAAGAA AAGGCTCTCT 
GGTAAGCTGG GCCGCTCGGA GAGCCTACGA GTTTACGAGC GCAAGCGTTC CCAGCGCGGC 
TCCTCCAAAG CCAAGCAGAC ACGTTCCCGT AGCGACGTTG ACCTGGAGGC AGCTGCCCGA 
GCCAACGACT TGCAGGGCAC CACTGGCAAG CTTGGGCCAA AGCCTGGCAA CTACACCCCA 
GAGCCTGGAG GGAGTTCAGA GGGCCCGCTG CCCTTTCTGC TTCCCCAGTC TGAGGAGACT 
GAGCCGCGCG TTCAGGAGGC CGAGAGCGAC CCCCCAAACT GGAGGGAGCT CGTCCCCCCT 
GAAATACTCC TCAGGCTGAA GAAAAGCGAA ATCAAGCGGC AAGAGGTCAT CAATGAGCTC 
TTCTTTACAG AGCACGCTCA TGTACGGATG CTTCGTGTGT TGCATGATGT CTTCTACATC 
CCCATGCAGA ATGGATGGAT CCCCACCTTG GAGCTGGAGA ACATCTTTCC TAGCCTGGAT 
GACCTTATCG AGGCTCATGC TGTATTCCTG GACACCTTGA AGCGTCTGCG CCTTGAGAAT 
AACTCAGTGA TCCAGGAAAT AGGCACCATC TTGCTGAACC GGTTTGACGG CACAGAGGGC 
AACTGGTTTC AGAAGATTTC ATCTCGGTTC TGCAGCCGGC AGCCATTTGC CTTGGAACAG 
TTGAAAGCGA AACAGAAGAG AGACCCTCGA TTTACGCAGT TTATTCAGGA AGCTGAGAGC 
AGCCCTCACT GCCGGCGGTT ACAACTAAAG GACATTATAC CATTTGAGAT GCAAAGGCTG 
ACCAAGTACC CGTTGCTGCT GCAGAACATC GCAAATCTAA CTGAGGAAGA CGAGGAGAAG 
CAGAGAGTGC AGAGGGCAGC AGAGTCTTGC AGGCAGATCC TAAACCATGT TAACCAGGAG 
GTGCGCGAGA TGGAGAATAA GATGAAACTT GCAGATTACC AAAGACGCCT GGATGTTTCC 
AACCTGAGGC AAATCAACGA CCCCTTATTT AATGAATTTA AGAACATTGA TATCAGCAAA 
AAGAATTTGA TACATGAAGG ATCTTTGACA TGGAAAGTGA GCAGGGACAA GGCTTTTGAT 
GTCCATGTCC TCCTCCTGGA TGATCTCTTG ATGCTGCTGC TGAAACAAGA TGAGCGGCTG 
GTACTGAAGT GCCAGAGTCG GACTATTATT CCTGCTCCAG ACGGGAAGCT CATGCTGAGC 
CCAATCATCA AGCTGAATAT GGCCATGGTC AGAGAGGTGG CTACAGATAA GAATGCCTTT 
TTTGTGATTT TCAACTGGGA GGATCGAGCA CAGTTCTATG AACTTGTGGC TCCAACACCC 
GCTGAGAAGA AAAACTGGTT TTTACTGATC AGCAACACTA CAGGCTTATT GGAACCCCCA 
GCTTCTTCCA AACAGAGACA CAGATCTATC ACTCCTGGCA CAATGCCGCA GAGCCCCTCC 
GACTTTGCAG ATCAGCTGCT GAGCAGCTCA GAAAATGGAG GCTCCCTGAA AGATTCAGTA 
TCCAAAGATG AAAAGGAAGA GTCGGTGGAG GATGCATTGG AAGGTCAGGA AGAGAAGGAC 
TTCAGCGGCC TTACAGAATA CCTCCTTGCC AGCAACATTG ATCTGAACCA AGATGCAGCA 
TCTGGAGGAG GCAATGTTGC CAGCGAGGCC TTGGAAGAAG TGTCGGTGCT AAAACGCCTG 
CTAGTGCGCA ATATCCGCCT CAGTGTGGAT GCAGACACGC TCTCAACCCT GGAGACCTGG 
TCAGCGGGGG AAGAACCTGC ACTGGAGGCT TCTGAACAGG GAAGGGCCTC CAATGGGGAT 
CGAGAGGCAG AGGATTCTGA AATGGAATCT ACACAACAGG GAGAGAATGG CAGAAAGCAG 
AGTTCTGCAG ATGTTGCAGA GTCCCTGTGC GCACCCATTG TACTGTCCAA AGAACAGTCG 
GAGGAGGTGA AGAGGCACAT TCTGTCACTG GAGCAGAGGA TCCAGCAGCT TAAGCTGATC 
GAGGAAGATT ACAACAACCT GCACAGAACA ATCGCCAAAT TCACTGTACC GAGGAGCAGC 
TTTACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgef1 ( XM_012967441.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012822895.3 Xenopus tropicalis Rho guanine nucleotide exchange factor 1 (arhgef1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa62572
Clone ID Related Accession (Same CDS sequence)	XM_012967441.3
Accession Version	XM_012967441.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3387bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho guanine nucleotide exchange factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012967441.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTCACT CTGTCTACCT CTTACCCTCC TGCATGAGTT ATTGTGGATT CCAAAAGCAC 
GGCTCACAGG TTTCTCCTGG TCTGTGCATC ACTTATGCAT CAGCCGACCT GAGCAGGGGA 
TTCCCATTGT CTCACTCACT GGGTTTTTGC CAGACTACTC CCAAACCCCT CGTCTGCCAG 
ATAAGAAGCA TGGATCCGGA CGATGGCCAT TTTGGGGGTT TGTACTCCAA GGGGTCCATG 
TCGATCATTG GAGCAGAGGA TGAGGATTTT GAGAATGACA TTGAACCGAA TATGGTTGAT 
GAGAGCAGTT ACTTCCGAAG CATTGAGCTA GTGAAAGAAC ACCCGGCCTA CATTATGGTG 
TTCTTGCAGC ACGTGGTTCT TCAGTTTGAC CCCAGCCCAC TGCTCTGTTT CCTGCATGCG 
GAGATGCTAA AGAACCTTTC AGCCAAAGAG CTTAAGAAAG TCTTCATGGA TTTTCACTAC 
ACATTTCTGA TGAAAGGAGC GGTACTGCAT GTCAGTGTGC CAAACAGCGT ATCCTTTGAA 
CTGGATCGGA CAAGGCCTGA GATGATCCCA GAAGAAACCC AGAGAAAATA TGTCAAAGAC 
ATTCAGAAGC ACCAAACTGA GGCCATTTCC CAACAGCTGG AGGACTTTAG GGCCAAGCGA 
GTACTTGGTA TGACCCCTGG GGAACTGGAG CTGAGCGAGC TTGAATCCTA TCGAACCAAC 
GACCGCAGTA TCCTGGAAGC CAAAGAGAAG CAGTTGGCAG AGCAGCTCCT GGCACGGCTG 
GATGAGATGC ACCTAACTAT TGCTCCAGAC GAGGAAAAAA GCTCTGCCAT CTTTTGTGCC 
ATTGTAGCCT ACATGAAGCA CCTGGGGGTG AAGACCAAGG CGGCGGATAG CAAAAAATCT 
AAAGGAATCT TCTTCAGGAA AAAGGTTTCT ACAAATAAAA AGGATGAGCC TCTGAAAACA 
AAGAAAACAT TCAGCATCTT GGATCCTATG CGCTGGAACC GAAATGATTC TCACTATGTT 
GACAGCAGAC CATCCAAAGT TGAGCCCGAA GCTGAGAAGC CAAACCCGGA CAGAAGGGTC 
AGCCAGGGGC AGAGAACTTT TGATAGGAGT GACAGCAGCA CAGCGAAATC CAAGAACAGC 
GAAGGGCCTG ATTCTGTGGT GTCTGTAAAT ATCATCCCCC CTCCTAATGA GCCCAATGAT 
CCTGACCCAG CAGTGACTGA TGGGGCATTG TCTAATGATG CATCTGAGGT CCTCAAAGGA 
TCCCCCACCA CTGAGCATTG CCCAGCAGAC CAGACTGAGG AAAGTGCAGA AAATGAAAGA 
CGAAAGAAAA GGCTCTCTGG TAAGCTGGGC CGCTCGGAGA GCCTACGAGT TTACGAGCGC 
AAGCGTTCCC AGCGCGGCTC CTCCAAAGCC AAGCAGACAC GTTCCCGTAG CGACGTTGAC 
CTGGAGGCAG CTGCCCGAGC CAACGACTTG CAGGGCACCA CTGGCAAGCT TGGGCCAAAG 
CCTGGCAACT ACACCCCAGA GCCTGGAGGG AGTTCAGAGG GCCCGCTGCC CTTTCTGCTT 
CCCCAGTCTG AGGAGACTGA GCCGCGCGTT CAGGAGGCCG AGAGCGACCC CCCAAACTGG 
AGGGAGCTCG TCCCCCCTGA AATACTCCTC AGGCTGAAGA AAAGCGAAAT CAAGCGGCAA 
GAGGTCATCA ATGAGCTCTT CTTTACAGAG CACGCTCATG TACGGATGCT TCGTGTGTTG 
CATGATGTCT TCTACATCCC CATGCAGAAT GGATGGATCC CCACCTTGGA GCTGGAGAAC 
ATCTTTCCTA GCCTGGATGA CCTTATCGAG GCTCATGCTG TATTCCTGGA CACCTTGAAG 
CGTCTGCGCC TTGAGAATAA CTCAGTGATC CAGGAAATAG GCACCATCTT GCTGAACCGG 
TTTGACGGCA CAGAGGGCAA CTGGTTTCAG AAGATTTCAT CTCGGTTCTG CAGCCGGCAG 
CCATTTGCCT TGGAACAGTT GAAAGCGAAA CAGAAGAGAG ACCCTCGATT TACGCAGTTT 
ATTCAGGAAG CTGAGAGCAG CCCTCACTGC CGGCGGTTAC AACTAAAGGA CATTATACCA 
TTTGAGATGC AAAGGCTGAC CAAGTACCCG TTGCTGCTGC AGAACATCGC AAATCTAACT 
GAGGAAGACG AGGAGAAGCA GAGAGTGCAG AGGGCAGCAG AGTCTTGCAG GCAGATCCTA 
AACCATGTTA ACCAGGAGGT GCGCGAGATG GAGAATAAGA TGAAACTTAC AGATTACCAA 
AGACGCCTGG ATGTTTCCAA CCTGAGGCAA ATCAACGACC CCTTATTTAA TGAATTTAAG 
AACATTGATA TCAGCAAAAA GAATTTGATA CATGAAGGAT CTTTGACATG GAAAGTGAGC 
AGGGACAAGG CTTTTGATGT CCATGTCCTC CTCCTGGATG ATCTCTTGAT GCTGCTGCTG 
AAACAAGATG AGCGGCTGGT ACTGAAGTGC CAGAGTCGGA CTATTATTCC TGCTCCAGAC 
GGGAAGCTCA TGCTGAGCCC AATCATCAAG CTGAATATGG CCATGGTCAG AGAGGTGGCT 
ACAGATAAGA ATGCCTTTTT TGTGATTTTC AACTGGGAGG ATCGAGCACA GTTCTATGAA 
CTTGTGGCTC CAACACCCGC TGAGAAGAAA AACTGGTTTT TACTGATCAG CAACACTACA 
GGCTTATTGG AACCCCCAGC TTCTTCCAAA CAGAGACACA GATCTATCAC TCCTGGCACA 
ATGCCGCAGA GCCCCTCCGA CTTTGCAGAT CAGCTGCTGA GCAGCTCAGA AAATGGAGGC 
TCCCTGAAAG ATTCAGTATC CAAAGATGAA AAGGAAGAGT CGGTGGAGGA TGCATTGGAG 
GGTCAGGAAG AGAAGGACTT CAGCGGCCTT ACAGAATACC TCCTTGCCAG CAACATTGAT 
CTGAACCAAG ATGCAGCATC TGGAGGAGGC AATGTTGCCA GCGAGGCCTT GGAAGAAGTG 
TCGGTGCTAA AACGCCTGCT AGTGCGCAAT ATCCGCCTCA GTGTGGATGC AGACACGCTC 
TCAACCCTGG AGACCTGGTC AGCGGGGGAA GAACCTGCAC TGGAGGCTTC TGAACAGGGA 
AGGGCCTCCA ATGGGGATCG AGAGGCAGAG GATTCTGAAA TGGAATCTAC ACAACAGGGA 
GAGAATGGCA GAAAGCAGAG TTCTGCAGAT GTTTCAGAGT CCCTGTGCGC ACCCATTGTA 
CTGTCCAAAG AACAGTCGGA GGAGGTGAAG AGGCACATTC TGTCACTGGA GCAGAGGATC 
CAGCAGCTTA AGCTGATCGA GGAAGATTAC AACAACCTGC ACAGAACAAT CGCCAAATTC 
ACTGTACCGA GGAGCAGCTT TACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012822895.3
CDS	16..3402
Translation	MTHSVYLLPSCMSYCGFQKHGSQVSPGLCITYASADLSRGFPLSHSLGFCQTTPKPLVCQIRSMDPDDGHFGGLYSKGSMSIIGAEDEDFENDIEPNMVDESSYFRSIELVKEHPAYIMVFLQHVVLQFDPSPLLCFLHAEMLKNLSAKELKKVFMDFHYTFLMKGAVLHVSVPNSVSFELDRTRPEMIPEETQRKYVKDIQKHQTEAISQQLEDFRAKRVLGMTPGELELSELESYRTNDRSILEAKEKQLAEQLLARLDEMHLTIAPDEEKSSAIFCAIVAYMKHLGVKTKAADSKKSKGIFFRKKVSTNKKDEPLKTKKTFSILDPMRWNRNDSHYVDSRPSKVEPEAEKPNPDRRVSQGQRTFDRSDSSTAKSKNSEGPDSVVSVNIIPPPNEPNDPDPAVTDGALSNDASEVLKGSPTTEHCPADQTEESAENERRKKRLSGKLGRSESLRVYERKRSQRGSSKAKQTRSRSDVDLEAAARANDLQGTTGKLGPKPGNYTPEPGGSSEGPLPFLLPQSEETEPRVQEAESDPPNWRELVPPEILLRLKKSEIKRQEVINELFFTEHAHVRMLRVLHDVFYIPMQNGWIPTLELENIFPSLDDLIEAHAVFLDTLKRLRLENNSVIQEIGTILLNRFDGTEGNWFQKISSRFCSRQPFALEQLKAKQKRDPRFTQFIQEAESSPHCRRLQLKDIIPFEMQRLTKYPLLLQNIANLTEEDEEKQRVQRAAESCRQILNHVNQEVREMENKMKLTDYQRRLDVSNLRQINDPLFNEFKNIDISKKNLIHEGSLTWKVSRDKAFDVHVLLLDDLLMLLLKQDERLVLKCQSRTIIPAPDGKLMLSPIIKLNMAMVREVATDKNAFFVIFNWEDRAQFYELVAPTPAEKKNWFLLISNTTGLLEPPASSKQRHRSITPGTMPQSPSDFADQLLSSSENGGSLKDSVSKDEKEESVEDALEGQEEKDFSGLTEYLLASNIDLNQDAASGGGNVASEALEEVSVLKRLLVRNIRLSVDADTLSTLETWSAGEEPALEASEQGRASNGDREAEDSEMESTQQGENGRKQSSADVSESLCAPIVLSKEQSEEVKRHILSLEQRIQQLKLIEEDYNNLHRTIAKFTVPRSSFT

Target ORF information:

RefSeq Version	XM_012967441.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho guanine nucleotide exchange factor 1 (arhgef1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012967441.3

ORF Insert Sequence:

ATGACTCACT CTGTCTACCT CTTACCCTCC TGCATGAGTT ATTGTGGATT CCAAAAGCAC 
GGCTCACAGG TTTCTCCTGG TCTGTGCATC ACTTATGCAT CAGCCGACCT GAGCAGGGGA 
TTCCCATTGT CTCACTCACT GGGTTTTTGC CAGACTACTC CCAAACCCCT CGTCTGCCAG 
ATAAGAAGCA TGGATCCGGA CGATGGCCAT TTTGGGGGTT TGTACTCCAA GGGGTCCATG 
TCGATCATTG GAGCAGAGGA TGAGGATTTT GAGAATGACA TTGAACCGAA TATGGTTGAT 
GAGAGCAGTT ACTTCCGAAG CATTGAGCTA GTGAAAGAAC ACCCGGCCTA CATTATGGTG 
TTCTTGCAGC ACGTGGTTCT TCAGTTTGAC CCCAGCCCAC TGCTCTGTTT CCTGCATGCG 
GAGATGCTAA AGAACCTTTC AGCCAAAGAG CTTAAGAAAG TCTTCATGGA TTTTCACTAC 
ACATTTCTGA TGAAAGGAGC GGTACTGCAT GTCAGTGTGC CAAACAGCGT ATCCTTTGAA 
CTGGATCGGA CAAGGCCTGA GATGATCCCA GAAGAAACCC AGAGAAAATA TGTCAAAGAC 
ATTCAGAAGC ACCAAACTGA GGCCATTTCC CAACAGCTGG AGGACTTTAG GGCCAAGCGA 
GTACTTGGTA TGACCCCTGG GGAACTGGAG CTGAGCGAGC TTGAATCCTA TCGAACCAAC 
GACCGCAGTA TCCTGGAAGC CAAAGAGAAG CAGTTGGCAG AGCAGCTCCT GGCACGGCTG 
GATGAGATGC ACCTAACTAT TGCTCCAGAC GAGGAAAAAA GCTCTGCCAT CTTTTGTGCC 
ATTGTAGCCT ACATGAAGCA CCTGGGGGTG AAGACCAAGG CGGCGGATAG CAAAAAATCT 
AAAGGAATCT TCTTCAGGAA AAAGGTTTCT ACAAATAAAA AGGATGAGCC TCTGAAAACA 
AAGAAAACAT TCAGCATCTT GGATCCTATG CGCTGGAACC GAAATGATTC TCACTATGTT 
GACAGCAGAC CATCCAAAGT TGAGCCCGAA GCTGAGAAGC CAAACCCGGA CAGAAGGGTC 
AGCCAGGGGC AGAGAACTTT TGATAGGAGT GACAGCAGCA CAGCGAAATC CAAGAACAGC 
GAAGGGCCTG ATTCTGTGGT GTCTGTAAAT ATCATCCCCC CTCCTAATGA GCCCAATGAT 
CCTGACCCAG CAGTGACTGA TGGGGCATTG TCTAATGATG CATCTGAGGT CCTCAAAGGA 
TCCCCCACCA CTGAGCATTG CCCAGCAGAC CAGACTGAGG AAAGTGCAGA AAATGAAAGA 
CGAAAGAAAA GGCTCTCTGG TAAGCTGGGC CGCTCGGAGA GCCTACGAGT TTACGAGCGC 
AAGCGTTCCC AGCGCGGCTC CTCCAAAGCC AAGCAGACAC GTTCCCGTAG CGACGTTGAC 
CTGGAGGCAG CTGCCCGAGC CAACGACTTG CAGGGCACCA CTGGCAAGCT TGGGCCAAAG 
CCTGGCAACT ACACCCCAGA GCCTGGAGGG AGTTCAGAGG GCCCGCTGCC CTTTCTGCTT 
CCCCAGTCTG AGGAGACTGA GCCGCGCGTT CAGGAGGCCG AGAGCGACCC CCCAAACTGG 
AGGGAGCTCG TCCCCCCTGA AATACTCCTC AGGCTGAAGA AAAGCGAAAT CAAGCGGCAA 
GAGGTCATCA ATGAGCTCTT CTTTACAGAG CACGCTCATG TACGGATGCT TCGTGTGTTG 
CATGATGTCT TCTACATCCC CATGCAGAAT GGATGGATCC CCACCTTGGA GCTGGAGAAC 
ATCTTTCCTA GCCTGGATGA CCTTATCGAG GCTCATGCTG TATTCCTGGA CACCTTGAAG 
CGTCTGCGCC TTGAGAATAA CTCAGTGATC CAGGAAATAG GCACCATCTT GCTGAACCGG 
TTTGACGGCA CAGAGGGCAA CTGGTTTCAG AAGATTTCAT CTCGGTTCTG CAGCCGGCAG 
CCATTTGCCT TGGAACAGTT GAAAGCGAAA CAGAAGAGAG ACCCTCGATT TACGCAGTTT 
ATTCAGGAAG CTGAGAGCAG CCCTCACTGC CGGCGGTTAC AACTAAAGGA CATTATACCA 
TTTGAGATGC AAAGGCTGAC CAAGTACCCG TTGCTGCTGC AGAACATCGC AAATCTAACT 
GAGGAAGACG AGGAGAAGCA GAGAGTGCAG AGGGCAGCAG AGTCTTGCAG GCAGATCCTA 
AACCATGTTA ACCAGGAGGT GCGCGAGATG GAGAATAAGA TGAAACTTAC AGATTACCAA 
AGACGCCTGG ATGTTTCCAA CCTGAGGCAA ATCAACGACC CCTTATTTAA TGAATTTAAG 
AACATTGATA TCAGCAAAAA GAATTTGATA CATGAAGGAT CTTTGACATG GAAAGTGAGC 
AGGGACAAGG CTTTTGATGT CCATGTCCTC CTCCTGGATG ATCTCTTGAT GCTGCTGCTG 
AAACAAGATG AGCGGCTGGT ACTGAAGTGC CAGAGTCGGA CTATTATTCC TGCTCCAGAC 
GGGAAGCTCA TGCTGAGCCC AATCATCAAG CTGAATATGG CCATGGTCAG AGAGGTGGCT 
ACAGATAAGA ATGCCTTTTT TGTGATTTTC AACTGGGAGG ATCGAGCACA GTTCTATGAA 
CTTGTGGCTC CAACACCCGC TGAGAAGAAA AACTGGTTTT TACTGATCAG CAACACTACA 
GGCTTATTGG AACCCCCAGC TTCTTCCAAA CAGAGACACA GATCTATCAC TCCTGGCACA 
ATGCCGCAGA GCCCCTCCGA CTTTGCAGAT CAGCTGCTGA GCAGCTCAGA AAATGGAGGC 
TCCCTGAAAG ATTCAGTATC CAAAGATGAA AAGGAAGAGT CGGTGGAGGA TGCATTGGAG 
GGTCAGGAAG AGAAGGACTT CAGCGGCCTT ACAGAATACC TCCTTGCCAG CAACATTGAT 
CTGAACCAAG ATGCAGCATC TGGAGGAGGC AATGTTGCCA GCGAGGCCTT GGAAGAAGTG 
TCGGTGCTAA AACGCCTGCT AGTGCGCAAT ATCCGCCTCA GTGTGGATGC AGACACGCTC 
TCAACCCTGG AGACCTGGTC AGCGGGGGAA GAACCTGCAC TGGAGGCTTC TGAACAGGGA 
AGGGCCTCCA ATGGGGATCG AGAGGCAGAG GATTCTGAAA TGGAATCTAC ACAACAGGGA 
GAGAATGGCA GAAAGCAGAG TTCTGCAGAT GTTTCAGAGT CCCTGTGCGC ACCCATTGTA 
CTGTCCAAAG AACAGTCGGA GGAGGTGAAG AGGCACATTC TGTCACTGGA GCAGAGGATC 
CAGCAGCTTA AGCTGATCGA GGAAGATTAC AACAACCTGC ACAGAACAAT CGCCAAATTC 
ACTGTACCGA GGAGCAGCTT TACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgef1 ( XM_012967442.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012822896.3 Xenopus tropicalis Rho guanine nucleotide exchange factor 1 (arhgef1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa62573
Clone ID Related Accession (Same CDS sequence)	XM_012967442.3
Accession Version	XM_012967442.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3384bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho guanine nucleotide exchange factor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012967442.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTCACT CTGTCTACCT CTTACCCTCC TGCATGAGTT ATTGTGGATT CCAAAAGCAC 
GGCTCACAGG TTTCTCCTGG TCTGTGCATC ACTTATGCAT CAGCCGACCT GAGCAGGGGA 
TTCCCATTGT CTCACTCACT GGGTTTTTGC CAGACTACTC CCAAACCCCT CGTCTGCCAG 
ATAAGAAGCA TGGATCCGGA CGATGGCCAT TTTGGGGGTT TGTACTCCAA GGGGTCCATG 
TCGATCATTG GAGCAGAGGA TGAGGATTTT GAGAATGACA TTGAACCGAA TATGGTTGAT 
GAGAGCAGTT ACTTCCGAAG CATTGAGCTA GTGAAAGAAC ACCCGGCCTA CATTATGGTG 
TTCTTGCAGC ACGTGGTTCT TCAGTTTGAC CCCAGCCCAC TGCTCTGTTT CCTGCATGCG 
GAGATGCTAA AGAACCTTTC AGCCAAAGAG CTTAAGAAAG TCTTCATGGA TTTTCACTAC 
ACATTTCTGA TGAAAGGAGC GGTACTGCAT GTCAGTGTGC CAAACAGCGT ATCCTTTGAA 
CTGGATCGGA CAAGGCCTGA GATGATCCCA GAAGAAACCC AGAGAAAATA TGTCAAAGAC 
ATTCAGAAGC ACCAAACTGA GGCCATTTCC CAACAGCTGG AGGACTTTAG GGCCAAGCGA 
GTACTTGGTA TGACCCCTGG GGAACTGGAG CTGAGCGAGC TTGAATCCTA TCGAACCAAC 
GACCGCAGTA TCCTGGAAGC CAAAGAGAAG CAGTTGGCAG AGCAGCTCCT GGCACGGCTG 
GATGAGATGC ACCTAACTAT TGCTCCAGAC GAGGAAAAAA GCTCTGCCAT CTTTTGTGCC 
ATTGTAGCCT ACATGAAGCA CCTGGGGGTG AAGACCAAGG CGGCGGATAG CAAAAAATCT 
AAAGGAATCT TCTTCAGGAA AAAGGTTTCT ACAAATAAAA AGGATGAGCC TCTGAAAACA 
AAGAAAACAT TCAGCATCTT GGATCCTATG CGCTGGAACC GAAATGATTC TCACTATGTT 
GACAGCAGAC CATCCAAAGT TGAGCCCGAA GCTGAGAAGC CAAACCCGGA CAGAAGGGTC 
AGCCAGGGGC AGAGAACTTT TGATAGGAGT GACAGCAGCA CAGCGAAATC CAAGAACAGC 
GAAGGGCCTG ATTCTGTGGT GTCTGTAAAT ATCATCCCCC CTCCTAATGA GCCCAATGAT 
CCTGACCCAG TGACTGATGG GGCATTGTCT AATGATGCAT CTGAGGTCCT CAAAGGATCC 
CCCACCACTG AGCATTGCCC AGCAGACCAG ACTGAGGAAA GTGCAGAAAA TGAAAGACGA 
AAGAAAAGGC TCTCTGGTAA GCTGGGCCGC TCGGAGAGCC TACGAGTTTA CGAGCGCAAG 
CGTTCCCAGC GCGGCTCCTC CAAAGCCAAG CAGACACGTT CCCGTAGCGA CGTTGACCTG 
GAGGCAGCTG CCCGAGCCAA CGACTTGCAG GGCACCACTG GCAAGCTTGG GCCAAAGCCT 
GGCAACTACA CCCCAGAGCC TGGAGGGAGT TCAGAGGGCC CGCTGCCCTT TCTGCTTCCC 
CAGTCTGAGG AGACTGAGCC GCGCGTTCAG GAGGCCGAGA GCGACCCCCC AAACTGGAGG 
GAGCTCGTCC CCCCTGAAAT ACTCCTCAGG CTGAAGAAAA GCGAAATCAA GCGGCAAGAG 
GTCATCAATG AGCTCTTCTT TACAGAGCAC GCTCATGTAC GGATGCTTCG TGTGTTGCAT 
GATGTCTTCT ACATCCCCAT GCAGAATGGA TGGATCCCCA CCTTGGAGCT GGAGAACATC 
TTTCCTAGCC TGGATGACCT TATCGAGGCT CATGCTGTAT TCCTGGACAC CTTGAAGCGT 
CTGCGCCTTG AGAATAACTC AGTGATCCAG GAAATAGGCA CCATCTTGCT GAACCGGTTT 
GACGGCACAG AGGGCAACTG GTTTCAGAAG ATTTCATCTC GGTTCTGCAG CCGGCAGCCA 
TTTGCCTTGG AACAGTTGAA AGCGAAACAG AAGAGAGACC CTCGATTTAC GCAGTTTATT 
CAGGAAGCTG AGAGCAGCCC TCACTGCCGG CGGTTACAAC TAAAGGACAT TATACCATTT 
GAGATGCAAA GGCTGACCAA GTACCCGTTG CTGCTGCAGA ACATCGCAAA TCTAACTGAG 
GAAGACGAGG AGAAGCAGAG AGTGCAGAGG GCAGCAGAGT CTTGCAGGCA GATCCTAAAC 
CATGTTAACC AGGAGGTGCG CGAGATGGAG AATAAGATGA AACTTACAGA TTACCAAAGA 
CGCCTGGATG TTTCCAACCT GAGGCAAATC AACGACCCCT TATTTAATGA ATTTAAGAAC 
ATTGATATCA GCAAAAAGAA TTTGATACAT GAAGGATCTT TGACATGGAA AGTGAGCAGG 
GACAAGGCTT TTGATGTCCA TGTCCTCCTC CTGGATGATC TCTTGATGCT GCTGCTGAAA 
CAAGATGAGC GGCTGGTACT GAAGTGCCAG AGTCGGACTA TTATTCCTGC TCCAGACGGG 
AAGCTCATGC TGAGCCCAAT CATCAAGCTG AATATGGCCA TGGTCAGAGA GGTGGCTACA 
GATAAGAATG CCTTTTTTGT GATTTTCAAC TGGGAGGATC GAGCACAGTT CTATGAACTT 
GTGGCTCCAA CACCCGCTGA GAAGAAAAAC TGGTTTTTAC TGATCAGCAA CACTACAGGC 
TTATTGGAAC CCCCAGCTTC TTCCAAACAG AGACACAGAT CTATCACTCC TGGCACAATG 
CCGCAGAGCC CCTCCGACTT TGCAGATCAG CTGCTGAGCA GCTCAGAAAA TGGAGGCTCC 
CTGAAAGATT CAGTATCCAA AGATGAAAAG GAAGAGTCGG TGGAGGATGC ATTGGAGGGT 
CAGGAAGAGA AGGACTTCAG CGGCCTTACA GAATACCTCC TTGCCAGCAA CATTGATCTG 
AACCAAGATG CAGCATCTGG AGGAGGCAAT GTTGCCAGCG AGGCCTTGGA AGAAGTGTCG 
GTGCTAAAAC GCCTGCTAGT GCGCAATATC CGCCTCAGTG TGGATGCAGA CACGCTCTCA 
ACCCTGGAGA CCTGGTCAGC GGGGGAAGAA CCTGCACTGG AGGCTTCTGA ACAGGGAAGG 
GCCTCCAATG GGGATCGAGA GGCAGAGGAT TCTGAAATGG AATCTACACA ACAGGGAGAG 
AATGGCAGAA AGCAGAGTTC TGCAGATGTT TCAGAGTCCC TGTGCGCACC CATTGTACTG 
TCCAAAGAAC AGTCGGAGGA GGTGAAGAGG CACATTCTGT CACTGGAGCA GAGGATCCAG 
CAGCTTAAGC TGATCGAGGA AGATTACAAC AACCTGCACA GAACAATCGC CAAATTCACT 
GTACCGAGGA GCAGCTTTAC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012822896.3
CDS	16..3399
Translation	MTHSVYLLPSCMSYCGFQKHGSQVSPGLCITYASADLSRGFPLSHSLGFCQTTPKPLVCQIRSMDPDDGHFGGLYSKGSMSIIGAEDEDFENDIEPNMVDESSYFRSIELVKEHPAYIMVFLQHVVLQFDPSPLLCFLHAEMLKNLSAKELKKVFMDFHYTFLMKGAVLHVSVPNSVSFELDRTRPEMIPEETQRKYVKDIQKHQTEAISQQLEDFRAKRVLGMTPGELELSELESYRTNDRSILEAKEKQLAEQLLARLDEMHLTIAPDEEKSSAIFCAIVAYMKHLGVKTKAADSKKSKGIFFRKKVSTNKKDEPLKTKKTFSILDPMRWNRNDSHYVDSRPSKVEPEAEKPNPDRRVSQGQRTFDRSDSSTAKSKNSEGPDSVVSVNIIPPPNEPNDPDPVTDGALSNDASEVLKGSPTTEHCPADQTEESAENERRKKRLSGKLGRSESLRVYERKRSQRGSSKAKQTRSRSDVDLEAAARANDLQGTTGKLGPKPGNYTPEPGGSSEGPLPFLLPQSEETEPRVQEAESDPPNWRELVPPEILLRLKKSEIKRQEVINELFFTEHAHVRMLRVLHDVFYIPMQNGWIPTLELENIFPSLDDLIEAHAVFLDTLKRLRLENNSVIQEIGTILLNRFDGTEGNWFQKISSRFCSRQPFALEQLKAKQKRDPRFTQFIQEAESSPHCRRLQLKDIIPFEMQRLTKYPLLLQNIANLTEEDEEKQRVQRAAESCRQILNHVNQEVREMENKMKLTDYQRRLDVSNLRQINDPLFNEFKNIDISKKNLIHEGSLTWKVSRDKAFDVHVLLLDDLLMLLLKQDERLVLKCQSRTIIPAPDGKLMLSPIIKLNMAMVREVATDKNAFFVIFNWEDRAQFYELVAPTPAEKKNWFLLISNTTGLLEPPASSKQRHRSITPGTMPQSPSDFADQLLSSSENGGSLKDSVSKDEKEESVEDALEGQEEKDFSGLTEYLLASNIDLNQDAASGGGNVASEALEEVSVLKRLLVRNIRLSVDADTLSTLETWSAGEEPALEASEQGRASNGDREAEDSEMESTQQGENGRKQSSADVSESLCAPIVLSKEQSEEVKRHILSLEQRIQQLKLIEEDYNNLHRTIAKFTVPRSSFT

Target ORF information:

RefSeq Version	XM_012967442.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho guanine nucleotide exchange factor 1 (arhgef1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012967442.3

ORF Insert Sequence:

ATGACTCACT CTGTCTACCT CTTACCCTCC TGCATGAGTT ATTGTGGATT CCAAAAGCAC 
GGCTCACAGG TTTCTCCTGG TCTGTGCATC ACTTATGCAT CAGCCGACCT GAGCAGGGGA 
TTCCCATTGT CTCACTCACT GGGTTTTTGC CAGACTACTC CCAAACCCCT CGTCTGCCAG 
ATAAGAAGCA TGGATCCGGA CGATGGCCAT TTTGGGGGTT TGTACTCCAA GGGGTCCATG 
TCGATCATTG GAGCAGAGGA TGAGGATTTT GAGAATGACA TTGAACCGAA TATGGTTGAT 
GAGAGCAGTT ACTTCCGAAG CATTGAGCTA GTGAAAGAAC ACCCGGCCTA CATTATGGTG 
TTCTTGCAGC ACGTGGTTCT TCAGTTTGAC CCCAGCCCAC TGCTCTGTTT CCTGCATGCG 
GAGATGCTAA AGAACCTTTC AGCCAAAGAG CTTAAGAAAG TCTTCATGGA TTTTCACTAC 
ACATTTCTGA TGAAAGGAGC GGTACTGCAT GTCAGTGTGC CAAACAGCGT ATCCTTTGAA 
CTGGATCGGA CAAGGCCTGA GATGATCCCA GAAGAAACCC AGAGAAAATA TGTCAAAGAC 
ATTCAGAAGC ACCAAACTGA GGCCATTTCC CAACAGCTGG AGGACTTTAG GGCCAAGCGA 
GTACTTGGTA TGACCCCTGG GGAACTGGAG CTGAGCGAGC TTGAATCCTA TCGAACCAAC 
GACCGCAGTA TCCTGGAAGC CAAAGAGAAG CAGTTGGCAG AGCAGCTCCT GGCACGGCTG 
GATGAGATGC ACCTAACTAT TGCTCCAGAC GAGGAAAAAA GCTCTGCCAT CTTTTGTGCC 
ATTGTAGCCT ACATGAAGCA CCTGGGGGTG AAGACCAAGG CGGCGGATAG CAAAAAATCT 
AAAGGAATCT TCTTCAGGAA AAAGGTTTCT ACAAATAAAA AGGATGAGCC TCTGAAAACA 
AAGAAAACAT TCAGCATCTT GGATCCTATG CGCTGGAACC GAAATGATTC TCACTATGTT 
GACAGCAGAC CATCCAAAGT TGAGCCCGAA GCTGAGAAGC CAAACCCGGA CAGAAGGGTC 
AGCCAGGGGC AGAGAACTTT TGATAGGAGT GACAGCAGCA CAGCGAAATC CAAGAACAGC 
GAAGGGCCTG ATTCTGTGGT GTCTGTAAAT ATCATCCCCC CTCCTAATGA GCCCAATGAT 
CCTGACCCAG TGACTGATGG GGCATTGTCT AATGATGCAT CTGAGGTCCT CAAAGGATCC 
CCCACCACTG AGCATTGCCC AGCAGACCAG ACTGAGGAAA GTGCAGAAAA TGAAAGACGA 
AAGAAAAGGC TCTCTGGTAA GCTGGGCCGC TCGGAGAGCC TACGAGTTTA CGAGCGCAAG 
CGTTCCCAGC GCGGCTCCTC CAAAGCCAAG CAGACACGTT CCCGTAGCGA CGTTGACCTG 
GAGGCAGCTG CCCGAGCCAA CGACTTGCAG GGCACCACTG GCAAGCTTGG GCCAAAGCCT 
GGCAACTACA CCCCAGAGCC TGGAGGGAGT TCAGAGGGCC CGCTGCCCTT TCTGCTTCCC 
CAGTCTGAGG AGACTGAGCC GCGCGTTCAG GAGGCCGAGA GCGACCCCCC AAACTGGAGG 
GAGCTCGTCC CCCCTGAAAT ACTCCTCAGG CTGAAGAAAA GCGAAATCAA GCGGCAAGAG 
GTCATCAATG AGCTCTTCTT TACAGAGCAC GCTCATGTAC GGATGCTTCG TGTGTTGCAT 
GATGTCTTCT ACATCCCCAT GCAGAATGGA TGGATCCCCA CCTTGGAGCT GGAGAACATC 
TTTCCTAGCC TGGATGACCT TATCGAGGCT CATGCTGTAT TCCTGGACAC CTTGAAGCGT 
CTGCGCCTTG AGAATAACTC AGTGATCCAG GAAATAGGCA CCATCTTGCT GAACCGGTTT 
GACGGCACAG AGGGCAACTG GTTTCAGAAG ATTTCATCTC GGTTCTGCAG CCGGCAGCCA 
TTTGCCTTGG AACAGTTGAA AGCGAAACAG AAGAGAGACC CTCGATTTAC GCAGTTTATT 
CAGGAAGCTG AGAGCAGCCC TCACTGCCGG CGGTTACAAC TAAAGGACAT TATACCATTT 
GAGATGCAAA GGCTGACCAA GTACCCGTTG CTGCTGCAGA ACATCGCAAA TCTAACTGAG 
GAAGACGAGG AGAAGCAGAG AGTGCAGAGG GCAGCAGAGT CTTGCAGGCA GATCCTAAAC 
CATGTTAACC AGGAGGTGCG CGAGATGGAG AATAAGATGA AACTTACAGA TTACCAAAGA 
CGCCTGGATG TTTCCAACCT GAGGCAAATC AACGACCCCT TATTTAATGA ATTTAAGAAC 
ATTGATATCA GCAAAAAGAA TTTGATACAT GAAGGATCTT TGACATGGAA AGTGAGCAGG 
GACAAGGCTT TTGATGTCCA TGTCCTCCTC CTGGATGATC TCTTGATGCT GCTGCTGAAA 
CAAGATGAGC GGCTGGTACT GAAGTGCCAG AGTCGGACTA TTATTCCTGC TCCAGACGGG 
AAGCTCATGC TGAGCCCAAT CATCAAGCTG AATATGGCCA TGGTCAGAGA GGTGGCTACA 
GATAAGAATG CCTTTTTTGT GATTTTCAAC TGGGAGGATC GAGCACAGTT CTATGAACTT 
GTGGCTCCAA CACCCGCTGA GAAGAAAAAC TGGTTTTTAC TGATCAGCAA CACTACAGGC 
TTATTGGAAC CCCCAGCTTC TTCCAAACAG AGACACAGAT CTATCACTCC TGGCACAATG 
CCGCAGAGCC CCTCCGACTT TGCAGATCAG CTGCTGAGCA GCTCAGAAAA TGGAGGCTCC 
CTGAAAGATT CAGTATCCAA AGATGAAAAG GAAGAGTCGG TGGAGGATGC ATTGGAGGGT 
CAGGAAGAGA AGGACTTCAG CGGCCTTACA GAATACCTCC TTGCCAGCAA CATTGATCTG 
AACCAAGATG CAGCATCTGG AGGAGGCAAT GTTGCCAGCG AGGCCTTGGA AGAAGTGTCG 
GTGCTAAAAC GCCTGCTAGT GCGCAATATC CGCCTCAGTG TGGATGCAGA CACGCTCTCA 
ACCCTGGAGA CCTGGTCAGC GGGGGAAGAA CCTGCACTGG AGGCTTCTGA ACAGGGAAGG 
GCCTCCAATG GGGATCGAGA GGCAGAGGAT TCTGAAATGG AATCTACACA ACAGGGAGAG 
AATGGCAGAA AGCAGAGTTC TGCAGATGTT TCAGAGTCCC TGTGCGCACC CATTGTACTG 
TCCAAAGAAC AGTCGGAGGA GGTGAAGAGG CACATTCTGT CACTGGAGCA GAGGATCCAG 
CAGCTTAAGC TGATCGAGGA AGATTACAAC AACCTGCACA GAACAATCGC CAAATTCACT 
GTACCGAGGA GCAGCTTTAC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgef1 ( XM_012967443.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012822897.2 Xenopus tropicalis Rho guanine nucleotide exchange factor 1 (arhgef1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa62574
Clone ID Related Accession (Same CDS sequence)	XM_012967443.3
Accession Version	XM_012967443.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3375bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho guanine nucleotide exchange factor 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012967443.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTCACT CTGTCTACCT CTTACCCTCC TGCATGAGTT ATTGTGGATT CCAAAAGCAC 
GGCTCACAGG TTTCTCCTGG TCTGTGCATC ACTTATGCAT CAGCCGACCT GAGCAGGGGA 
TTCCCATTGT CTCACTCACT GGGTTTTTGC CAGACTACTC CCAAACCCCT CGTCTGCCAG 
ATAAGAAGCA TGGATCCGGA CGATGGCCAT TTTGGGGGTT TGTACTCCAA GGGGTCCATG 
TCGATCATTG GAGCAGAGGA TGAGGATTTT GAGAATGACA TTGAACCGAA TATGGTTGAT 
GAGAGCAGTT ACTTCCGAAG CATTGAGCTA GTGAAAGAAC ACCCGGCCTA CATTATGGTG 
TTCTTGCAGC ACGTGGTTCT TCAGTTTGAC CCCAGCCCAC TGCTCTGTTT CCTGCATGCG 
GAGATGCTAA AGAACCTTTC AGCCAAAGAG CTTAAGAAAG TCTTCATGGA TTTTCACTAC 
ACATTTCTGA TGAAAGGAGC GGTACTGCAT GTCAGTGTGC CAAACAGCGT ATCCTTTGAA 
CTGGATCGGA CAAGGCCTGA GATGATCCCA GAAGAAACCC AGAGAAAATA TGTCAAAGAC 
ATTCAGAAGC ACCAAACTGA GGCCATTTCC CAACAGCTGG AGGACTTTAG GGCCAAGCGA 
GTACTTGGTA TGACCCCTGG GGAACTGGAG CTGAGCGAGC TTGAATCCTA TCGAACCAAC 
GACCGCAGTA TCCTGGAAGC CAAAGAGAAG CAGTTGGCAG AGCAGCTCCT GGCACGGCTG 
GATGAGATGC ACCTAACTAT TGCTCCAGAC GAGGAAAAAA GCTCTGCCAT CTTTTGTGCC 
ATTGTAGCCT ACATGAAGCA CCTGGGGGTG AAGACCAAGG CGGCGGATAG CAAAAAATCT 
AAAGGAATCT TCTTCAGGAA AAAGGTTTCT ACAAATAAAA AGGATGAGCC TCTGAAAACA 
AAGAAAACAT TCAGCATCTT GGATCCTATG CGCTGGAACC GAAATGATTC TCACTATGTT 
GACAGCAGAC CATCCAAAGT TGAGCCCGAA GCTGAGAAGC CAAACCCGGA CAGAAGGGTC 
AGCCAGGGGC AGAGAACTTT TGATAGGAGT GACAGCAGCA CAGCGAAATC CAAGAACAGC 
GAAGGGCCTG ATTCTGTGGT GTCTGTAAAT ATCATCCCCC CTCCTAATGA GCCCAATGAT 
CCTGACCCAG CAGTGACTGA TGGGGCATTG TCTAATGATG CATCTGAGGT CCTCAAAGGA 
TCCCCCACCA CTGAGCATTG CCCAGCAGAC CAGACTGAGG AAAGTGCAGA AAATGAAAGG 
CTCTCTGGTA AGCTGGGCCG CTCGGAGAGC CTACGAGTTT ACGAGCGCAA GCGTTCCCAG 
CGCGGCTCCT CCAAAGCCAA GCAGACACGT TCCCGTAGCG ACGTTGACCT GGAGGCAGCT 
GCCCGAGCCA ACGACTTGCA GGGCACCACT GGCAAGCTTG GGCCAAAGCC TGGCAACTAC 
ACCCCAGAGC CTGGAGGGAG TTCAGAGGGC CCGCTGCCCT TTCTGCTTCC CCAGTCTGAG 
GAGACTGAGC CGCGCGTTCA GGAGGCCGAG AGCGACCCCC CAAACTGGAG GGAGCTCGTC 
CCCCCTGAAA TACTCCTCAG GCTGAAGAAA AGCGAAATCA AGCGGCAAGA GGTCATCAAT 
GAGCTCTTCT TTACAGAGCA CGCTCATGTA CGGATGCTTC GTGTGTTGCA TGATGTCTTC 
TACATCCCCA TGCAGAATGG ATGGATCCCC ACCTTGGAGC TGGAGAACAT CTTTCCTAGC 
CTGGATGACC TTATCGAGGC TCATGCTGTA TTCCTGGACA CCTTGAAGCG TCTGCGCCTT 
GAGAATAACT CAGTGATCCA GGAAATAGGC ACCATCTTGC TGAACCGGTT TGACGGCACA 
GAGGGCAACT GGTTTCAGAA GATTTCATCT CGGTTCTGCA GCCGGCAGCC ATTTGCCTTG 
GAACAGTTGA AAGCGAAACA GAAGAGAGAC CCTCGATTTA CGCAGTTTAT TCAGGAAGCT 
GAGAGCAGCC CTCACTGCCG GCGGTTACAA CTAAAGGACA TTATACCATT TGAGATGCAA 
AGGCTGACCA AGTACCCGTT GCTGCTGCAG AACATCGCAA ATCTAACTGA GGAAGACGAG 
GAGAAGCAGA GAGTGCAGAG GGCAGCAGAG TCTTGCAGGC AGATCCTAAA CCATGTTAAC 
CAGGAGGTGC GCGAGATGGA GAATAAGATG AAACTTACAG ATTACCAAAG ACGCCTGGAT 
GTTTCCAACC TGAGGCAAAT CAACGACCCC TTATTTAATG AATTTAAGAA CATTGATATC 
AGCAAAAAGA ATTTGATACA TGAAGGATCT TTGACATGGA AAGTGAGCAG GGACAAGGCT 
TTTGATGTCC ATGTCCTCCT CCTGGATGAT CTCTTGATGC TGCTGCTGAA ACAAGATGAG 
CGGCTGGTAC TGAAGTGCCA GAGTCGGACT ATTATTCCTG CTCCAGACGG GAAGCTCATG 
CTGAGCCCAA TCATCAAGCT GAATATGGCC ATGGTCAGAG AGGTGGCTAC AGATAAGAAT 
GCCTTTTTTG TGATTTTCAA CTGGGAGGAT CGAGCACAGT TCTATGAACT TGTGGCTCCA 
ACACCCGCTG AGAAGAAAAA CTGGTTTTTA CTGATCAGCA ACACTACAGG CTTATTGGAA 
CCCCCAGCTT CTTCCAAACA GAGACACAGA TCTATCACTC CTGGCACAAT GCCGCAGAGC 
CCCTCCGACT TTGCAGATCA GCTGCTGAGC AGCTCAGAAA ATGGAGGCTC CCTGAAAGAT 
TCAGTATCCA AAGATGAAAA GGAAGAGTCG GTGGAGGATG CATTGGAGGG TCAGGAAGAG 
AAGGACTTCA GCGGCCTTAC AGAATACCTC CTTGCCAGCA ACATTGATCT GAACCAAGAT 
GCAGCATCTG GAGGAGGCAA TGTTGCCAGC GAGGCCTTGG AAGAAGTGTC GGTGCTAAAA 
CGCCTGCTAG TGCGCAATAT CCGCCTCAGT GTGGATGCAG ACACGCTCTC AACCCTGGAG 
ACCTGGTCAG CGGGGGAAGA ACCTGCACTG GAGGCTTCTG AACAGGGAAG GGCCTCCAAT 
GGGGATCGAG AGGCAGAGGA TTCTGAAATG GAATCTACAC AACAGGGAGA GAATGGCAGA 
AAGCAGAGTT CTGCAGATGT TTCAGAGTCC CTGTGCGCAC CCATTGTACT GTCCAAAGAA 
CAGTCGGAGG AGGTGAAGAG GCACATTCTG TCACTGGAGC AGAGGATCCA GCAGCTTAAG 
CTGATCGAGG AAGATTACAA CAACCTGCAC AGAACAATCG CCAAATTCAC TGTACCGAGG 
AGCAGCTTTA CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012822897.2
CDS	16..3390
Translation	MTHSVYLLPSCMSYCGFQKHGSQVSPGLCITYASADLSRGFPLSHSLGFCQTTPKPLVCQIRSMDPDDGHFGGLYSKGSMSIIGAEDEDFENDIEPNMVDESSYFRSIELVKEHPAYIMVFLQHVVLQFDPSPLLCFLHAEMLKNLSAKELKKVFMDFHYTFLMKGAVLHVSVPNSVSFELDRTRPEMIPEETQRKYVKDIQKHQTEAISQQLEDFRAKRVLGMTPGELELSELESYRTNDRSILEAKEKQLAEQLLARLDEMHLTIAPDEEKSSAIFCAIVAYMKHLGVKTKAADSKKSKGIFFRKKVSTNKKDEPLKTKKTFSILDPMRWNRNDSHYVDSRPSKVEPEAEKPNPDRRVSQGQRTFDRSDSSTAKSKNSEGPDSVVSVNIIPPPNEPNDPDPAVTDGALSNDASEVLKGSPTTEHCPADQTEESAENERLSGKLGRSESLRVYERKRSQRGSSKAKQTRSRSDVDLEAAARANDLQGTTGKLGPKPGNYTPEPGGSSEGPLPFLLPQSEETEPRVQEAESDPPNWRELVPPEILLRLKKSEIKRQEVINELFFTEHAHVRMLRVLHDVFYIPMQNGWIPTLELENIFPSLDDLIEAHAVFLDTLKRLRLENNSVIQEIGTILLNRFDGTEGNWFQKISSRFCSRQPFALEQLKAKQKRDPRFTQFIQEAESSPHCRRLQLKDIIPFEMQRLTKYPLLLQNIANLTEEDEEKQRVQRAAESCRQILNHVNQEVREMENKMKLTDYQRRLDVSNLRQINDPLFNEFKNIDISKKNLIHEGSLTWKVSRDKAFDVHVLLLDDLLMLLLKQDERLVLKCQSRTIIPAPDGKLMLSPIIKLNMAMVREVATDKNAFFVIFNWEDRAQFYELVAPTPAEKKNWFLLISNTTGLLEPPASSKQRHRSITPGTMPQSPSDFADQLLSSSENGGSLKDSVSKDEKEESVEDALEGQEEKDFSGLTEYLLASNIDLNQDAASGGGNVASEALEEVSVLKRLLVRNIRLSVDADTLSTLETWSAGEEPALEASEQGRASNGDREAEDSEMESTQQGENGRKQSSADVSESLCAPIVLSKEQSEEVKRHILSLEQRIQQLKLIEEDYNNLHRTIAKFTVPRSSFT

Target ORF information:

RefSeq Version	XM_012967443.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho guanine nucleotide exchange factor 1 (arhgef1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012967443.3

ORF Insert Sequence:

ATGACTCACT CTGTCTACCT CTTACCCTCC TGCATGAGTT ATTGTGGATT CCAAAAGCAC 
GGCTCACAGG TTTCTCCTGG TCTGTGCATC ACTTATGCAT CAGCCGACCT GAGCAGGGGA 
TTCCCATTGT CTCACTCACT GGGTTTTTGC CAGACTACTC CCAAACCCCT CGTCTGCCAG 
ATAAGAAGCA TGGATCCGGA CGATGGCCAT TTTGGGGGTT TGTACTCCAA GGGGTCCATG 
TCGATCATTG GAGCAGAGGA TGAGGATTTT GAGAATGACA TTGAACCGAA TATGGTTGAT 
GAGAGCAGTT ACTTCCGAAG CATTGAGCTA GTGAAAGAAC ACCCGGCCTA CATTATGGTG 
TTCTTGCAGC ACGTGGTTCT TCAGTTTGAC CCCAGCCCAC TGCTCTGTTT CCTGCATGCG 
GAGATGCTAA AGAACCTTTC AGCCAAAGAG CTTAAGAAAG TCTTCATGGA TTTTCACTAC 
ACATTTCTGA TGAAAGGAGC GGTACTGCAT GTCAGTGTGC CAAACAGCGT ATCCTTTGAA 
CTGGATCGGA CAAGGCCTGA GATGATCCCA GAAGAAACCC AGAGAAAATA TGTCAAAGAC 
ATTCAGAAGC ACCAAACTGA GGCCATTTCC CAACAGCTGG AGGACTTTAG GGCCAAGCGA 
GTACTTGGTA TGACCCCTGG GGAACTGGAG CTGAGCGAGC TTGAATCCTA TCGAACCAAC 
GACCGCAGTA TCCTGGAAGC CAAAGAGAAG CAGTTGGCAG AGCAGCTCCT GGCACGGCTG 
GATGAGATGC ACCTAACTAT TGCTCCAGAC GAGGAAAAAA GCTCTGCCAT CTTTTGTGCC 
ATTGTAGCCT ACATGAAGCA CCTGGGGGTG AAGACCAAGG CGGCGGATAG CAAAAAATCT 
AAAGGAATCT TCTTCAGGAA AAAGGTTTCT ACAAATAAAA AGGATGAGCC TCTGAAAACA 
AAGAAAACAT TCAGCATCTT GGATCCTATG CGCTGGAACC GAAATGATTC TCACTATGTT 
GACAGCAGAC CATCCAAAGT TGAGCCCGAA GCTGAGAAGC CAAACCCGGA CAGAAGGGTC 
AGCCAGGGGC AGAGAACTTT TGATAGGAGT GACAGCAGCA CAGCGAAATC CAAGAACAGC 
GAAGGGCCTG ATTCTGTGGT GTCTGTAAAT ATCATCCCCC CTCCTAATGA GCCCAATGAT 
CCTGACCCAG CAGTGACTGA TGGGGCATTG TCTAATGATG CATCTGAGGT CCTCAAAGGA 
TCCCCCACCA CTGAGCATTG CCCAGCAGAC CAGACTGAGG AAAGTGCAGA AAATGAAAGG 
CTCTCTGGTA AGCTGGGCCG CTCGGAGAGC CTACGAGTTT ACGAGCGCAA GCGTTCCCAG 
CGCGGCTCCT CCAAAGCCAA GCAGACACGT TCCCGTAGCG ACGTTGACCT GGAGGCAGCT 
GCCCGAGCCA ACGACTTGCA GGGCACCACT GGCAAGCTTG GGCCAAAGCC TGGCAACTAC 
ACCCCAGAGC CTGGAGGGAG TTCAGAGGGC CCGCTGCCCT TTCTGCTTCC CCAGTCTGAG 
GAGACTGAGC CGCGCGTTCA GGAGGCCGAG AGCGACCCCC CAAACTGGAG GGAGCTCGTC 
CCCCCTGAAA TACTCCTCAG GCTGAAGAAA AGCGAAATCA AGCGGCAAGA GGTCATCAAT 
GAGCTCTTCT TTACAGAGCA CGCTCATGTA CGGATGCTTC GTGTGTTGCA TGATGTCTTC 
TACATCCCCA TGCAGAATGG ATGGATCCCC ACCTTGGAGC TGGAGAACAT CTTTCCTAGC 
CTGGATGACC TTATCGAGGC TCATGCTGTA TTCCTGGACA CCTTGAAGCG TCTGCGCCTT 
GAGAATAACT CAGTGATCCA GGAAATAGGC ACCATCTTGC TGAACCGGTT TGACGGCACA 
GAGGGCAACT GGTTTCAGAA GATTTCATCT CGGTTCTGCA GCCGGCAGCC ATTTGCCTTG 
GAACAGTTGA AAGCGAAACA GAAGAGAGAC CCTCGATTTA CGCAGTTTAT TCAGGAAGCT 
GAGAGCAGCC CTCACTGCCG GCGGTTACAA CTAAAGGACA TTATACCATT TGAGATGCAA 
AGGCTGACCA AGTACCCGTT GCTGCTGCAG AACATCGCAA ATCTAACTGA GGAAGACGAG 
GAGAAGCAGA GAGTGCAGAG GGCAGCAGAG TCTTGCAGGC AGATCCTAAA CCATGTTAAC 
CAGGAGGTGC GCGAGATGGA GAATAAGATG AAACTTACAG ATTACCAAAG ACGCCTGGAT 
GTTTCCAACC TGAGGCAAAT CAACGACCCC TTATTTAATG AATTTAAGAA CATTGATATC 
AGCAAAAAGA ATTTGATACA TGAAGGATCT TTGACATGGA AAGTGAGCAG GGACAAGGCT 
TTTGATGTCC ATGTCCTCCT CCTGGATGAT CTCTTGATGC TGCTGCTGAA ACAAGATGAG 
CGGCTGGTAC TGAAGTGCCA GAGTCGGACT ATTATTCCTG CTCCAGACGG GAAGCTCATG 
CTGAGCCCAA TCATCAAGCT GAATATGGCC ATGGTCAGAG AGGTGGCTAC AGATAAGAAT 
GCCTTTTTTG TGATTTTCAA CTGGGAGGAT CGAGCACAGT TCTATGAACT TGTGGCTCCA 
ACACCCGCTG AGAAGAAAAA CTGGTTTTTA CTGATCAGCA ACACTACAGG CTTATTGGAA 
CCCCCAGCTT CTTCCAAACA GAGACACAGA TCTATCACTC CTGGCACAAT GCCGCAGAGC 
CCCTCCGACT TTGCAGATCA GCTGCTGAGC AGCTCAGAAA ATGGAGGCTC CCTGAAAGAT 
TCAGTATCCA AAGATGAAAA GGAAGAGTCG GTGGAGGATG CATTGGAGGG TCAGGAAGAG 
AAGGACTTCA GCGGCCTTAC AGAATACCTC CTTGCCAGCA ACATTGATCT GAACCAAGAT 
GCAGCATCTG GAGGAGGCAA TGTTGCCAGC GAGGCCTTGG AAGAAGTGTC GGTGCTAAAA 
CGCCTGCTAG TGCGCAATAT CCGCCTCAGT GTGGATGCAG ACACGCTCTC AACCCTGGAG 
ACCTGGTCAG CGGGGGAAGA ACCTGCACTG GAGGCTTCTG AACAGGGAAG GGCCTCCAAT 
GGGGATCGAG AGGCAGAGGA TTCTGAAATG GAATCTACAC AACAGGGAGA GAATGGCAGA 
AAGCAGAGTT CTGCAGATGT TTCAGAGTCC CTGTGCGCAC CCATTGTACT GTCCAAAGAA 
CAGTCGGAGG AGGTGAAGAG GCACATTCTG TCACTGGAGC AGAGGATCCA GCAGCTTAAG 
CTGATCGAGG AAGATTACAA CAACCTGCAC AGAACAATCG CCAAATTCAC TGTACCGAGG 
AGCAGCTTTA CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgef1 ( XM_012967444.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012822898.3 Xenopus tropicalis Rho guanine nucleotide exchange factor 1 (arhgef1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa62575
Clone ID Related Accession (Same CDS sequence)	XM_012967444.3
Accession Version	XM_012967444.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho guanine nucleotide exchange factor 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012967444.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTCACT CTGTCTACCT CTTACCCTCC TGCATGAGTT ATTGTGGATT CCAAAAGCAC 
GGCTCACAGG TTTCTCCTGG TCTGTGCATC ACTTATGCAT CAGCCGACCT GAGCAGGGGA 
TTCCCATTGT CTCACTCACT GGGTTTTTGC CAGACTACTC CCAAACCCCT CGTCTGCCAG 
ATAAGAAGCA TGGATCCGGA CGATGGCCAT TTTGGGGGTT TGTACTCCAA GGGGTCCATG 
TCGATCATTG GAGCAGAGGA TGAGGATTTT GAGAATGACA TTGAACCGAA TATGGTTGAT 
GAGAGCAGTT ACTTCCGAAG CATTGAGCTA GTGAAAGAAC ACCCGGCCTA CATTATGGTG 
TTCTTGCAGC ACGTGGTTCT TCAGTTTGAC CCCAGCCCAC TGCTCTGTTT CCTGCATGCG 
GAGATGCTAA AGAACCTTTC AGCCAAAGAG CTTAAGAAAG TCTTCATGGA TTTTCACTAC 
ACATTTCTGA TGAAAGGAGC GGTACTGCAT GTCAGTGTGC CAAACAGCGT ATCCTTTGAA 
CTGGATCGGA CAAGGCCTGA GATGATCCCA GAAGAAACCC AGAGAAAATA TGTCAAAGAC 
ATTCAGAAGC ACCAAACTGA GGCCATTTCC CAACAGCTGG AGGACTTTAG GGCCAAGCGA 
GTACTTGGTA TGACCCCTGG GGAACTGGAG CTGAGCGAGC TTGAATCCTA TCGAACCAAC 
GACCGCAGTA TCCTGGAAGC CAAAGAGAAG CAGTTGGCAG AGCAGCTCCT GGCACGGCTG 
GATGAGATGC ACCTAACTAT TGCTCCAGAC GAGGAAAAAA GCTCTGCCAT CTTTTGTGCC 
ATTGTAGCCT ACATGAAGCA CCTGGGGGTG AAGACCAAGG CGGCGGATAG CAAAAAATCT 
AAAGGAATCT TCTTCAGGAA AAAGGTTTCT ACAAATAAAA AGGATGAGCC TCTGAAAACA 
AAGAAAACAT TCAGCATCTT GGATCCTATG CGCTGGAACC GAAATGATTC TCACTCTGAG 
AAGCCAAACC CGGACAGAAG GGTCAGCCAG GGGCAGAGAA CTTTTGATAG GAGTGACAGC 
AGCACAGCGA AATCCAAGAA CAGCGAAGGG CCTGATTCTG TGGTGTCTGT AAATATCATC 
CCCCCTCCTA ATGAGCCCAA TGATCCTGAC CCAGCAGTGA CTGATGGGGC ATTGTCTAAT 
GATGCATCTG AGGTCCTCAA AGGATCCCCC ACCACTGAGC ATTGCCCAGC AGACCAGACT 
GAGGAAAGTG CAGAAAATGA AAGACGAAAG AAAAGGCTCT CTGGTAAGCT GGGCCGCTCG 
GAGAGCCTAC GAGTTTACGA GCGCAAGCGT TCCCAGCGCG GCTCCTCCAA AGCCAAGCAG 
ACACGTTCCC GTAGCGACGT TGACCTGGAG GCAGCTGCCC GAGCCAACGA CTTGCAGGGC 
ACCACTGGCA AGCTTGGGCC AAAGCCTGGC AACTACACCC CAGAGCCTGG AGGGAGTTCA 
GAGGGCCCGC TGCCCTTTCT GCTTCCCCAG TCTGAGGAGA CTGAGCCGCG CGTTCAGGAG 
GCCGAGAGCG ACCCCCCAAA CTGGAGGGAG CTCGTCCCCC CTGAAATACT CCTCAGGCTG 
AAGAAAAGCG AAATCAAGCG GCAAGAGGTC ATCAATGAGC TCTTCTTTAC AGAGCACGCT 
CATGTACGGA TGCTTCGTGT GTTGCATGAT GTCTTCTACA TCCCCATGCA GAATGGATGG 
ATCCCCACCT TGGAGCTGGA GAACATCTTT CCTAGCCTGG ATGACCTTAT CGAGGCTCAT 
GCTGTATTCC TGGACACCTT GAAGCGTCTG CGCCTTGAGA ATAACTCAGT GATCCAGGAA 
ATAGGCACCA TCTTGCTGAA CCGGTTTGAC GGCACAGAGG GCAACTGGTT TCAGAAGATT 
TCATCTCGGT TCTGCAGCCG GCAGCCATTT GCCTTGGAAC AGTTGAAAGC GAAACAGAAG 
AGAGACCCTC GATTTACGCA GTTTATTCAG GAAGCTGAGA GCAGCCCTCA CTGCCGGCGG 
TTACAACTAA AGGACATTAT ACCATTTGAG ATGCAAAGGC TGACCAAGTA CCCGTTGCTG 
CTGCAGAACA TCGCAAATCT AACTGAGGAA GACGAGGAGA AGCAGAGAGT GCAGAGGGCA 
GCAGAGTCTT GCAGGCAGAT CCTAAACCAT GTTAACCAGG AGGTGCGCGA GATGGAGAAT 
AAGATGAAAC TTACAGATTA CCAAAGACGC CTGGATGTTT CCAACCTGAG GCAAATCAAC 
GACCCCTTAT TTAATGAATT TAAGAACATT GATATCAGCA AAAAGAATTT GATACATGAA 
GGATCTTTGA CATGGAAAGT GAGCAGGGAC AAGGCTTTTG ATGTCCATGT CCTCCTCCTG 
GATGATCTCT TGATGCTGCT GCTGAAACAA GATGAGCGGC TGGTACTGAA GTGCCAGAGT 
CGGACTATTA TTCCTGCTCC AGACGGGAAG CTCATGCTGA GCCCAATCAT CAAGCTGAAT 
ATGGCCATGG TCAGAGAGGT GGCTACAGAT AAGAATGCCT TTTTTGTGAT TTTCAACTGG 
GAGGATCGAG CACAGTTCTA TGAACTTGTG GCTCCAACAC CCGCTGAGAA GAAAAACTGG 
TTTTTACTGA TCAGCAACAC TACAGGCTTA TTGGAACCCC CAGCTTCTTC CAAACAGAGA 
CACAGATCTA TCACTCCTGG CACAATGCCG CAGAGCCCCT CCGACTTTGC AGATCAGCTG 
CTGAGCAGCT CAGAAAATGG AGGCTCCCTG AAAGATTCAG TATCCAAAGA TGAAAAGGAA 
GAGTCGGTGG AGGATGCATT GGAGGGTCAG GAAGAGAAGG ACTTCAGCGG CCTTACAGAA 
TACCTCCTTG CCAGCAACAT TGATCTGAAC CAAGATGCAG CATCTGGAGG AGGCAATGTT 
GCCAGCGAGG CCTTGGAAGA AGTGTCGGTG CTAAAACGCC TGCTAGTGCG CAATATCCGC 
CTCAGTGTGG ATGCAGACAC GCTCTCAACC CTGGAGACCT GGTCAGCGGG GGAAGAACCT 
GCACTGGAGG CTTCTGAACA GGGAAGGGCC TCCAATGGGG ATCGAGAGGC AGAGGATTCT 
GAAATGGAAT CTACACAACA GGGAGAGAAT GGCAGAAAGC AGAGTTCTGC AGATGTTTCA 
GAGTCCCTGT GCGCACCCAT TGTACTGTCC AAAGAACAGT CGGAGGAGGT GAAGAGGCAC 
ATTCTGTCAC TGGAGCAGAG GATCCAGCAG CTTAAGCTGA TCGAGGAAGA TTACAACAAC 
CTGCACAGAA CAATCGCCAA ATTCACTGTA CCGAGGAGCA GCTTTACATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012822898.3
CDS	16..3366
Translation	MTHSVYLLPSCMSYCGFQKHGSQVSPGLCITYASADLSRGFPLSHSLGFCQTTPKPLVCQIRSMDPDDGHFGGLYSKGSMSIIGAEDEDFENDIEPNMVDESSYFRSIELVKEHPAYIMVFLQHVVLQFDPSPLLCFLHAEMLKNLSAKELKKVFMDFHYTFLMKGAVLHVSVPNSVSFELDRTRPEMIPEETQRKYVKDIQKHQTEAISQQLEDFRAKRVLGMTPGELELSELESYRTNDRSILEAKEKQLAEQLLARLDEMHLTIAPDEEKSSAIFCAIVAYMKHLGVKTKAADSKKSKGIFFRKKVSTNKKDEPLKTKKTFSILDPMRWNRNDSHSEKPNPDRRVSQGQRTFDRSDSSTAKSKNSEGPDSVVSVNIIPPPNEPNDPDPAVTDGALSNDASEVLKGSPTTEHCPADQTEESAENERRKKRLSGKLGRSESLRVYERKRSQRGSSKAKQTRSRSDVDLEAAARANDLQGTTGKLGPKPGNYTPEPGGSSEGPLPFLLPQSEETEPRVQEAESDPPNWRELVPPEILLRLKKSEIKRQEVINELFFTEHAHVRMLRVLHDVFYIPMQNGWIPTLELENIFPSLDDLIEAHAVFLDTLKRLRLENNSVIQEIGTILLNRFDGTEGNWFQKISSRFCSRQPFALEQLKAKQKRDPRFTQFIQEAESSPHCRRLQLKDIIPFEMQRLTKYPLLLQNIANLTEEDEEKQRVQRAAESCRQILNHVNQEVREMENKMKLTDYQRRLDVSNLRQINDPLFNEFKNIDISKKNLIHEGSLTWKVSRDKAFDVHVLLLDDLLMLLLKQDERLVLKCQSRTIIPAPDGKLMLSPIIKLNMAMVREVATDKNAFFVIFNWEDRAQFYELVAPTPAEKKNWFLLISNTTGLLEPPASSKQRHRSITPGTMPQSPSDFADQLLSSSENGGSLKDSVSKDEKEESVEDALEGQEEKDFSGLTEYLLASNIDLNQDAASGGGNVASEALEEVSVLKRLLVRNIRLSVDADTLSTLETWSAGEEPALEASEQGRASNGDREAEDSEMESTQQGENGRKQSSADVSESLCAPIVLSKEQSEEVKRHILSLEQRIQQLKLIEEDYNNLHRTIAKFTVPRSSFT

Target ORF information:

RefSeq Version	XM_012967444.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho guanine nucleotide exchange factor 1 (arhgef1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012967444.3

ORF Insert Sequence:

ATGACTCACT CTGTCTACCT CTTACCCTCC TGCATGAGTT ATTGTGGATT CCAAAAGCAC 
GGCTCACAGG TTTCTCCTGG TCTGTGCATC ACTTATGCAT CAGCCGACCT GAGCAGGGGA 
TTCCCATTGT CTCACTCACT GGGTTTTTGC CAGACTACTC CCAAACCCCT CGTCTGCCAG 
ATAAGAAGCA TGGATCCGGA CGATGGCCAT TTTGGGGGTT TGTACTCCAA GGGGTCCATG 
TCGATCATTG GAGCAGAGGA TGAGGATTTT GAGAATGACA TTGAACCGAA TATGGTTGAT 
GAGAGCAGTT ACTTCCGAAG CATTGAGCTA GTGAAAGAAC ACCCGGCCTA CATTATGGTG 
TTCTTGCAGC ACGTGGTTCT TCAGTTTGAC CCCAGCCCAC TGCTCTGTTT CCTGCATGCG 
GAGATGCTAA AGAACCTTTC AGCCAAAGAG CTTAAGAAAG TCTTCATGGA TTTTCACTAC 
ACATTTCTGA TGAAAGGAGC GGTACTGCAT GTCAGTGTGC CAAACAGCGT ATCCTTTGAA 
CTGGATCGGA CAAGGCCTGA GATGATCCCA GAAGAAACCC AGAGAAAATA TGTCAAAGAC 
ATTCAGAAGC ACCAAACTGA GGCCATTTCC CAACAGCTGG AGGACTTTAG GGCCAAGCGA 
GTACTTGGTA TGACCCCTGG GGAACTGGAG CTGAGCGAGC TTGAATCCTA TCGAACCAAC 
GACCGCAGTA TCCTGGAAGC CAAAGAGAAG CAGTTGGCAG AGCAGCTCCT GGCACGGCTG 
GATGAGATGC ACCTAACTAT TGCTCCAGAC GAGGAAAAAA GCTCTGCCAT CTTTTGTGCC 
ATTGTAGCCT ACATGAAGCA CCTGGGGGTG AAGACCAAGG CGGCGGATAG CAAAAAATCT 
AAAGGAATCT TCTTCAGGAA AAAGGTTTCT ACAAATAAAA AGGATGAGCC TCTGAAAACA 
AAGAAAACAT TCAGCATCTT GGATCCTATG CGCTGGAACC GAAATGATTC TCACTCTGAG 
AAGCCAAACC CGGACAGAAG GGTCAGCCAG GGGCAGAGAA CTTTTGATAG GAGTGACAGC 
AGCACAGCGA AATCCAAGAA CAGCGAAGGG CCTGATTCTG TGGTGTCTGT AAATATCATC 
CCCCCTCCTA ATGAGCCCAA TGATCCTGAC CCAGCAGTGA CTGATGGGGC ATTGTCTAAT 
GATGCATCTG AGGTCCTCAA AGGATCCCCC ACCACTGAGC ATTGCCCAGC AGACCAGACT 
GAGGAAAGTG CAGAAAATGA AAGACGAAAG AAAAGGCTCT CTGGTAAGCT GGGCCGCTCG 
GAGAGCCTAC GAGTTTACGA GCGCAAGCGT TCCCAGCGCG GCTCCTCCAA AGCCAAGCAG 
ACACGTTCCC GTAGCGACGT TGACCTGGAG GCAGCTGCCC GAGCCAACGA CTTGCAGGGC 
ACCACTGGCA AGCTTGGGCC AAAGCCTGGC AACTACACCC CAGAGCCTGG AGGGAGTTCA 
GAGGGCCCGC TGCCCTTTCT GCTTCCCCAG TCTGAGGAGA CTGAGCCGCG CGTTCAGGAG 
GCCGAGAGCG ACCCCCCAAA CTGGAGGGAG CTCGTCCCCC CTGAAATACT CCTCAGGCTG 
AAGAAAAGCG AAATCAAGCG GCAAGAGGTC ATCAATGAGC TCTTCTTTAC AGAGCACGCT 
CATGTACGGA TGCTTCGTGT GTTGCATGAT GTCTTCTACA TCCCCATGCA GAATGGATGG 
ATCCCCACCT TGGAGCTGGA GAACATCTTT CCTAGCCTGG ATGACCTTAT CGAGGCTCAT 
GCTGTATTCC TGGACACCTT GAAGCGTCTG CGCCTTGAGA ATAACTCAGT GATCCAGGAA 
ATAGGCACCA TCTTGCTGAA CCGGTTTGAC GGCACAGAGG GCAACTGGTT TCAGAAGATT 
TCATCTCGGT TCTGCAGCCG GCAGCCATTT GCCTTGGAAC AGTTGAAAGC GAAACAGAAG 
AGAGACCCTC GATTTACGCA GTTTATTCAG GAAGCTGAGA GCAGCCCTCA CTGCCGGCGG 
TTACAACTAA AGGACATTAT ACCATTTGAG ATGCAAAGGC TGACCAAGTA CCCGTTGCTG 
CTGCAGAACA TCGCAAATCT AACTGAGGAA GACGAGGAGA AGCAGAGAGT GCAGAGGGCA 
GCAGAGTCTT GCAGGCAGAT CCTAAACCAT GTTAACCAGG AGGTGCGCGA GATGGAGAAT 
AAGATGAAAC TTACAGATTA CCAAAGACGC CTGGATGTTT CCAACCTGAG GCAAATCAAC 
GACCCCTTAT TTAATGAATT TAAGAACATT GATATCAGCA AAAAGAATTT GATACATGAA 
GGATCTTTGA CATGGAAAGT GAGCAGGGAC AAGGCTTTTG ATGTCCATGT CCTCCTCCTG 
GATGATCTCT TGATGCTGCT GCTGAAACAA GATGAGCGGC TGGTACTGAA GTGCCAGAGT 
CGGACTATTA TTCCTGCTCC AGACGGGAAG CTCATGCTGA GCCCAATCAT CAAGCTGAAT 
ATGGCCATGG TCAGAGAGGT GGCTACAGAT AAGAATGCCT TTTTTGTGAT TTTCAACTGG 
GAGGATCGAG CACAGTTCTA TGAACTTGTG GCTCCAACAC CCGCTGAGAA GAAAAACTGG 
TTTTTACTGA TCAGCAACAC TACAGGCTTA TTGGAACCCC CAGCTTCTTC CAAACAGAGA 
CACAGATCTA TCACTCCTGG CACAATGCCG CAGAGCCCCT CCGACTTTGC AGATCAGCTG 
CTGAGCAGCT CAGAAAATGG AGGCTCCCTG AAAGATTCAG TATCCAAAGA TGAAAAGGAA 
GAGTCGGTGG AGGATGCATT GGAGGGTCAG GAAGAGAAGG ACTTCAGCGG CCTTACAGAA 
TACCTCCTTG CCAGCAACAT TGATCTGAAC CAAGATGCAG CATCTGGAGG AGGCAATGTT 
GCCAGCGAGG CCTTGGAAGA AGTGTCGGTG CTAAAACGCC TGCTAGTGCG CAATATCCGC 
CTCAGTGTGG ATGCAGACAC GCTCTCAACC CTGGAGACCT GGTCAGCGGG GGAAGAACCT 
GCACTGGAGG CTTCTGAACA GGGAAGGGCC TCCAATGGGG ATCGAGAGGC AGAGGATTCT 
GAAATGGAAT CTACACAACA GGGAGAGAAT GGCAGAAAGC AGAGTTCTGC AGATGTTTCA 
GAGTCCCTGT GCGCACCCAT TGTACTGTCC AAAGAACAGT CGGAGGAGGT GAAGAGGCAC 
ATTCTGTCAC TGGAGCAGAG GATCCAGCAG CTTAAGCTGA TCGAGGAAGA TTACAACAAC 
CTGCACAGAA CAATCGCCAA ATTCACTGTA CCGAGGAGCA GCTTTACATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgef1 ( XM_012967445.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012822899.3 Xenopus tropicalis Rho guanine nucleotide exchange factor 1 (arhgef1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OXa62576
Clone ID Related Accession (Same CDS sequence)	XM_012967445.3
Accession Version	XM_012967445.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3348bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho guanine nucleotide exchange factor 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012967445.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTCACT CTGTCTACCT CTTACCCTCC TGCATGAGTT ATTGTGGATT CCAAAAGCAC 
GGCTCACAGG TTTCTCCTGG TCTGTGCATC ACTTATGCAT CAGCCGACCT GAGCAGGGGA 
TTCCCATTGT CTCACTCACT GGGTTTTTGC CAGACTACTC CCAAACCCCT CGTCTGCCAG 
ATAAGAAGCA TGGATCCGGA CGATGGCCAT TTTGGGGGTT TGTACTCCAA GGGGTCCATG 
TCGATCATTG GAGCAGAGGA TGAGGATTTT GAGAATGACA TTGAACCGAA TATGGTTGAT 
GAGAGCAGTT ACTTCCGAAG CATTGAGCTA GTGAAAGAAC ACCCGGCCTA CATTATGGTG 
TTCTTGCAGC ACGTGGTTCT TCAGTTTGAC CCCAGCCCAC TGCTCTGTTT CCTGCATGCG 
GAGATGCTAA AGAACCTTTC AGCCAAAGAG CTTAAGAAAG TCTTCATGGA TTTTCACTAC 
ACATTTCTGA TGAAAGGAGC GGTACTGCAT GTCAGTGTGC CAAACAGCGT ATCCTTTGAA 
CTGGATCGGA CAAGGCCTGA GATGATCCCA GAAGAAACCC AGAGAAAATA TGTCAAAGAC 
ATTCAGAAGC ACCAAACTGA GGCCATTTCC CAACAGCTGG AGGACTTTAG GGCCAAGCGA 
GTACTTGGTA TGACCCCTGG GGAACTGGAG CTGAGCGAGC TTGAATCCTA TCGAACCAAC 
GACCGCAGTA TCCTGGAAGC CAAAGAGAAG CAGTTGGCAG AGCAGCTCCT GGCACGGCTG 
GATGAGATGC ACCTAACTAT TGCTCCAGAC GAGGAAAAAA GCTCTGCCAT CTTTTGTGCC 
ATTGTAGCCT ACATGAAGCA CCTGGGGGTG AAGACCAAGG CGGCGGATAG CAAAAAATCT 
AAAGGAATCT TCTTCAGGAA AAAGGTTTCT ACAAATAAAA AGGATGAGCC TCTGAAAACA 
AAGAAAACAT TCAGCATCTT GGATCCTATG CGCTGGAACC GAAATGATTC TCACTCTGAG 
AAGCCAAACC CGGACAGAAG GGTCAGCCAG GGGCAGAGAA CTTTTGATAG GAGTGACAGC 
AGCACAGCGA AATCCAAGAA CAGCGAAGGG CCTGATTCTG TGGTGTCTGT AAATATCATC 
CCCCCTCCTA ATGAGCCCAA TGATCCTGAC CCAGTGACTG ATGGGGCATT GTCTAATGAT 
GCATCTGAGG TCCTCAAAGG ATCCCCCACC ACTGAGCATT GCCCAGCAGA CCAGACTGAG 
GAAAGTGCAG AAAATGAAAG ACGAAAGAAA AGGCTCTCTG GTAAGCTGGG CCGCTCGGAG 
AGCCTACGAG TTTACGAGCG CAAGCGTTCC CAGCGCGGCT CCTCCAAAGC CAAGCAGACA 
CGTTCCCGTA GCGACGTTGA CCTGGAGGCA GCTGCCCGAG CCAACGACTT GCAGGGCACC 
ACTGGCAAGC TTGGGCCAAA GCCTGGCAAC TACACCCCAG AGCCTGGAGG GAGTTCAGAG 
GGCCCGCTGC CCTTTCTGCT TCCCCAGTCT GAGGAGACTG AGCCGCGCGT TCAGGAGGCC 
GAGAGCGACC CCCCAAACTG GAGGGAGCTC GTCCCCCCTG AAATACTCCT CAGGCTGAAG 
AAAAGCGAAA TCAAGCGGCA AGAGGTCATC AATGAGCTCT TCTTTACAGA GCACGCTCAT 
GTACGGATGC TTCGTGTGTT GCATGATGTC TTCTACATCC CCATGCAGAA TGGATGGATC 
CCCACCTTGG AGCTGGAGAA CATCTTTCCT AGCCTGGATG ACCTTATCGA GGCTCATGCT 
GTATTCCTGG ACACCTTGAA GCGTCTGCGC CTTGAGAATA ACTCAGTGAT CCAGGAAATA 
GGCACCATCT TGCTGAACCG GTTTGACGGC ACAGAGGGCA ACTGGTTTCA GAAGATTTCA 
TCTCGGTTCT GCAGCCGGCA GCCATTTGCC TTGGAACAGT TGAAAGCGAA ACAGAAGAGA 
GACCCTCGAT TTACGCAGTT TATTCAGGAA GCTGAGAGCA GCCCTCACTG CCGGCGGTTA 
CAACTAAAGG ACATTATACC ATTTGAGATG CAAAGGCTGA CCAAGTACCC GTTGCTGCTG 
CAGAACATCG CAAATCTAAC TGAGGAAGAC GAGGAGAAGC AGAGAGTGCA GAGGGCAGCA 
GAGTCTTGCA GGCAGATCCT AAACCATGTT AACCAGGAGG TGCGCGAGAT GGAGAATAAG 
ATGAAACTTA CAGATTACCA AAGACGCCTG GATGTTTCCA ACCTGAGGCA AATCAACGAC 
CCCTTATTTA ATGAATTTAA GAACATTGAT ATCAGCAAAA AGAATTTGAT ACATGAAGGA 
TCTTTGACAT GGAAAGTGAG CAGGGACAAG GCTTTTGATG TCCATGTCCT CCTCCTGGAT 
GATCTCTTGA TGCTGCTGCT GAAACAAGAT GAGCGGCTGG TACTGAAGTG CCAGAGTCGG 
ACTATTATTC CTGCTCCAGA CGGGAAGCTC ATGCTGAGCC CAATCATCAA GCTGAATATG 
GCCATGGTCA GAGAGGTGGC TACAGATAAG AATGCCTTTT TTGTGATTTT CAACTGGGAG 
GATCGAGCAC AGTTCTATGA ACTTGTGGCT CCAACACCCG CTGAGAAGAA AAACTGGTTT 
TTACTGATCA GCAACACTAC AGGCTTATTG GAACCCCCAG CTTCTTCCAA ACAGAGACAC 
AGATCTATCA CTCCTGGCAC AATGCCGCAG AGCCCCTCCG ACTTTGCAGA TCAGCTGCTG 
AGCAGCTCAG AAAATGGAGG CTCCCTGAAA GATTCAGTAT CCAAAGATGA AAAGGAAGAG 
TCGGTGGAGG ATGCATTGGA GGGTCAGGAA GAGAAGGACT TCAGCGGCCT TACAGAATAC 
CTCCTTGCCA GCAACATTGA TCTGAACCAA GATGCAGCAT CTGGAGGAGG CAATGTTGCC 
AGCGAGGCCT TGGAAGAAGT GTCGGTGCTA AAACGCCTGC TAGTGCGCAA TATCCGCCTC 
AGTGTGGATG CAGACACGCT CTCAACCCTG GAGACCTGGT CAGCGGGGGA AGAACCTGCA 
CTGGAGGCTT CTGAACAGGG AAGGGCCTCC AATGGGGATC GAGAGGCAGA GGATTCTGAA 
ATGGAATCTA CACAACAGGG AGAGAATGGC AGAAAGCAGA GTTCTGCAGA TGTTTCAGAG 
TCCCTGTGCG CACCCATTGT ACTGTCCAAA GAACAGTCGG AGGAGGTGAA GAGGCACATT 
CTGTCACTGG AGCAGAGGAT CCAGCAGCTT AAGCTGATCG AGGAAGATTA CAACAACCTG 
CACAGAACAA TCGCCAAATT CACTGTACCG AGGAGCAGCT TTACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012822899.3
CDS	16..3363
Translation	MTHSVYLLPSCMSYCGFQKHGSQVSPGLCITYASADLSRGFPLSHSLGFCQTTPKPLVCQIRSMDPDDGHFGGLYSKGSMSIIGAEDEDFENDIEPNMVDESSYFRSIELVKEHPAYIMVFLQHVVLQFDPSPLLCFLHAEMLKNLSAKELKKVFMDFHYTFLMKGAVLHVSVPNSVSFELDRTRPEMIPEETQRKYVKDIQKHQTEAISQQLEDFRAKRVLGMTPGELELSELESYRTNDRSILEAKEKQLAEQLLARLDEMHLTIAPDEEKSSAIFCAIVAYMKHLGVKTKAADSKKSKGIFFRKKVSTNKKDEPLKTKKTFSILDPMRWNRNDSHSEKPNPDRRVSQGQRTFDRSDSSTAKSKNSEGPDSVVSVNIIPPPNEPNDPDPVTDGALSNDASEVLKGSPTTEHCPADQTEESAENERRKKRLSGKLGRSESLRVYERKRSQRGSSKAKQTRSRSDVDLEAAARANDLQGTTGKLGPKPGNYTPEPGGSSEGPLPFLLPQSEETEPRVQEAESDPPNWRELVPPEILLRLKKSEIKRQEVINELFFTEHAHVRMLRVLHDVFYIPMQNGWIPTLELENIFPSLDDLIEAHAVFLDTLKRLRLENNSVIQEIGTILLNRFDGTEGNWFQKISSRFCSRQPFALEQLKAKQKRDPRFTQFIQEAESSPHCRRLQLKDIIPFEMQRLTKYPLLLQNIANLTEEDEEKQRVQRAAESCRQILNHVNQEVREMENKMKLTDYQRRLDVSNLRQINDPLFNEFKNIDISKKNLIHEGSLTWKVSRDKAFDVHVLLLDDLLMLLLKQDERLVLKCQSRTIIPAPDGKLMLSPIIKLNMAMVREVATDKNAFFVIFNWEDRAQFYELVAPTPAEKKNWFLLISNTTGLLEPPASSKQRHRSITPGTMPQSPSDFADQLLSSSENGGSLKDSVSKDEKEESVEDALEGQEEKDFSGLTEYLLASNIDLNQDAASGGGNVASEALEEVSVLKRLLVRNIRLSVDADTLSTLETWSAGEEPALEASEQGRASNGDREAEDSEMESTQQGENGRKQSSADVSESLCAPIVLSKEQSEEVKRHILSLEQRIQQLKLIEEDYNNLHRTIAKFTVPRSSFT

Target ORF information:

RefSeq Version	XM_012967445.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho guanine nucleotide exchange factor 1 (arhgef1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012967445.3

ORF Insert Sequence:

ATGACTCACT CTGTCTACCT CTTACCCTCC TGCATGAGTT ATTGTGGATT CCAAAAGCAC 
GGCTCACAGG TTTCTCCTGG TCTGTGCATC ACTTATGCAT CAGCCGACCT GAGCAGGGGA 
TTCCCATTGT CTCACTCACT GGGTTTTTGC CAGACTACTC CCAAACCCCT CGTCTGCCAG 
ATAAGAAGCA TGGATCCGGA CGATGGCCAT TTTGGGGGTT TGTACTCCAA GGGGTCCATG 
TCGATCATTG GAGCAGAGGA TGAGGATTTT GAGAATGACA TTGAACCGAA TATGGTTGAT 
GAGAGCAGTT ACTTCCGAAG CATTGAGCTA GTGAAAGAAC ACCCGGCCTA CATTATGGTG 
TTCTTGCAGC ACGTGGTTCT TCAGTTTGAC CCCAGCCCAC TGCTCTGTTT CCTGCATGCG 
GAGATGCTAA AGAACCTTTC AGCCAAAGAG CTTAAGAAAG TCTTCATGGA TTTTCACTAC 
ACATTTCTGA TGAAAGGAGC GGTACTGCAT GTCAGTGTGC CAAACAGCGT ATCCTTTGAA 
CTGGATCGGA CAAGGCCTGA GATGATCCCA GAAGAAACCC AGAGAAAATA TGTCAAAGAC 
ATTCAGAAGC ACCAAACTGA GGCCATTTCC CAACAGCTGG AGGACTTTAG GGCCAAGCGA 
GTACTTGGTA TGACCCCTGG GGAACTGGAG CTGAGCGAGC TTGAATCCTA TCGAACCAAC 
GACCGCAGTA TCCTGGAAGC CAAAGAGAAG CAGTTGGCAG AGCAGCTCCT GGCACGGCTG 
GATGAGATGC ACCTAACTAT TGCTCCAGAC GAGGAAAAAA GCTCTGCCAT CTTTTGTGCC 
ATTGTAGCCT ACATGAAGCA CCTGGGGGTG AAGACCAAGG CGGCGGATAG CAAAAAATCT 
AAAGGAATCT TCTTCAGGAA AAAGGTTTCT ACAAATAAAA AGGATGAGCC TCTGAAAACA 
AAGAAAACAT TCAGCATCTT GGATCCTATG CGCTGGAACC GAAATGATTC TCACTCTGAG 
AAGCCAAACC CGGACAGAAG GGTCAGCCAG GGGCAGAGAA CTTTTGATAG GAGTGACAGC 
AGCACAGCGA AATCCAAGAA CAGCGAAGGG CCTGATTCTG TGGTGTCTGT AAATATCATC 
CCCCCTCCTA ATGAGCCCAA TGATCCTGAC CCAGTGACTG ATGGGGCATT GTCTAATGAT 
GCATCTGAGG TCCTCAAAGG ATCCCCCACC ACTGAGCATT GCCCAGCAGA CCAGACTGAG 
GAAAGTGCAG AAAATGAAAG ACGAAAGAAA AGGCTCTCTG GTAAGCTGGG CCGCTCGGAG 
AGCCTACGAG TTTACGAGCG CAAGCGTTCC CAGCGCGGCT CCTCCAAAGC CAAGCAGACA 
CGTTCCCGTA GCGACGTTGA CCTGGAGGCA GCTGCCCGAG CCAACGACTT GCAGGGCACC 
ACTGGCAAGC TTGGGCCAAA GCCTGGCAAC TACACCCCAG AGCCTGGAGG GAGTTCAGAG 
GGCCCGCTGC CCTTTCTGCT TCCCCAGTCT GAGGAGACTG AGCCGCGCGT TCAGGAGGCC 
GAGAGCGACC CCCCAAACTG GAGGGAGCTC GTCCCCCCTG AAATACTCCT CAGGCTGAAG 
AAAAGCGAAA TCAAGCGGCA AGAGGTCATC AATGAGCTCT TCTTTACAGA GCACGCTCAT 
GTACGGATGC TTCGTGTGTT GCATGATGTC TTCTACATCC CCATGCAGAA TGGATGGATC 
CCCACCTTGG AGCTGGAGAA CATCTTTCCT AGCCTGGATG ACCTTATCGA GGCTCATGCT 
GTATTCCTGG ACACCTTGAA GCGTCTGCGC CTTGAGAATA ACTCAGTGAT CCAGGAAATA 
GGCACCATCT TGCTGAACCG GTTTGACGGC ACAGAGGGCA ACTGGTTTCA GAAGATTTCA 
TCTCGGTTCT GCAGCCGGCA GCCATTTGCC TTGGAACAGT TGAAAGCGAA ACAGAAGAGA 
GACCCTCGAT TTACGCAGTT TATTCAGGAA GCTGAGAGCA GCCCTCACTG CCGGCGGTTA 
CAACTAAAGG ACATTATACC ATTTGAGATG CAAAGGCTGA CCAAGTACCC GTTGCTGCTG 
CAGAACATCG CAAATCTAAC TGAGGAAGAC GAGGAGAAGC AGAGAGTGCA GAGGGCAGCA 
GAGTCTTGCA GGCAGATCCT AAACCATGTT AACCAGGAGG TGCGCGAGAT GGAGAATAAG 
ATGAAACTTA CAGATTACCA AAGACGCCTG GATGTTTCCA ACCTGAGGCA AATCAACGAC 
CCCTTATTTA ATGAATTTAA GAACATTGAT ATCAGCAAAA AGAATTTGAT ACATGAAGGA 
TCTTTGACAT GGAAAGTGAG CAGGGACAAG GCTTTTGATG TCCATGTCCT CCTCCTGGAT 
GATCTCTTGA TGCTGCTGCT GAAACAAGAT GAGCGGCTGG TACTGAAGTG CCAGAGTCGG 
ACTATTATTC CTGCTCCAGA CGGGAAGCTC ATGCTGAGCC CAATCATCAA GCTGAATATG 
GCCATGGTCA GAGAGGTGGC TACAGATAAG AATGCCTTTT TTGTGATTTT CAACTGGGAG 
GATCGAGCAC AGTTCTATGA ACTTGTGGCT CCAACACCCG CTGAGAAGAA AAACTGGTTT 
TTACTGATCA GCAACACTAC AGGCTTATTG GAACCCCCAG CTTCTTCCAA ACAGAGACAC 
AGATCTATCA CTCCTGGCAC AATGCCGCAG AGCCCCTCCG ACTTTGCAGA TCAGCTGCTG 
AGCAGCTCAG AAAATGGAGG CTCCCTGAAA GATTCAGTAT CCAAAGATGA AAAGGAAGAG 
TCGGTGGAGG ATGCATTGGA GGGTCAGGAA GAGAAGGACT TCAGCGGCCT TACAGAATAC 
CTCCTTGCCA GCAACATTGA TCTGAACCAA GATGCAGCAT CTGGAGGAGG CAATGTTGCC 
AGCGAGGCCT TGGAAGAAGT GTCGGTGCTA AAACGCCTGC TAGTGCGCAA TATCCGCCTC 
AGTGTGGATG CAGACACGCT CTCAACCCTG GAGACCTGGT CAGCGGGGGA AGAACCTGCA 
CTGGAGGCTT CTGAACAGGG AAGGGCCTCC AATGGGGATC GAGAGGCAGA GGATTCTGAA 
ATGGAATCTA CACAACAGGG AGAGAATGGC AGAAAGCAGA GTTCTGCAGA TGTTTCAGAG 
TCCCTGTGCG CACCCATTGT ACTGTCCAAA GAACAGTCGG AGGAGGTGAA GAGGCACATT 
CTGTCACTGG AGCAGAGGAT CCAGCAGCTT AAGCTGATCG AGGAAGATTA CAACAACCTG 
CACAGAACAA TCGCCAAATT CACTGTACCG AGGAGCAGCT TTACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgef1 ( XM_004916244.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004916301.3 Xenopus tropicalis Rho guanine nucleotide exchange factor 1 (arhgef1), transcript variant X6, mRNA.

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CloneID	OXa62577
Clone ID Related Accession (Same CDS sequence)	XM_004916244.4
Accession Version	XM_004916244.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3192bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho guanine nucleotide exchange factor 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004916244.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTCACT CTGTCTACCT CTTACCCTCC TGCATGAGTT ATTGTGGATT CCAAAAGCAC 
GGCTCACAGG TTTCTCCTGG TCTGTGCATC ACTTATGCAT CAGCCGACCT GAGCAGGGGA 
TTCCCATTGT CTCACTCACT GGGTTTTTGC CAGACTACTC CCAAACCCCT CGTCTGCCAG 
ATAAGAAGCA TGGATCCGGA CGATGGCCAT TTTGGGGGTT TGTACTCCAA GGGGTCCATG 
TCGATCATTG GAGCAGAGGA TGAGGATTTT GAGAATGACA TTGAACCGAA TATGGTTGAT 
GAGAGCAGTT ACTTCCGAAG CATTGAGCTA GTGAAAGAAC ACCCGGCCTA CATTATGGTG 
TTCTTGCAGC ACGTGGTTCT TCAGTTTGAC CCCAGCCCAC TGCTCTGTTT CCTGCATGCG 
GAGATGCTAA AGAACCTTTC AGCCAAAGAG CTTAAGAAAG TCTTCATGGA TTTTCACTAC 
ACATTTCTGA TGAAAGGAGC GGTACTGCAT GTCAGTGTGC CAAACAGCGT ATCCTTTGAA 
CTGGATCGGA CAAGGCCTGA GATGATCCCA GAAGAAACCC AGAGAAAATA TGTCAAAGAC 
ATTCAGAAGC ACCAAACTGA GGCCATTTCC CAACAGCTGG AGGACTTTAG GGCCAAGCGA 
GTACTTGGTA TGACCCCTGG GGAACTGGAG CTGAGCGAGC TTGAATCCTA TCGAACCAAC 
GACCGCAGTA TCCTGGAAGC CAAAGAGAAG CAGTTGGCAG AGCAGCTCCT GGCACGGCTG 
GATGAGATGC ACCTAACTAT TGCTCCAGAC GAGGAAAAAA GCTCTGCCAT CTTTTGTGCC 
ATTGTAGCCT ACATGAAGCA CCTGGGGGTG AAGACCAAGG CGGCGGATAG CAAAAAATCT 
AAAGGAATCT TCTTCAGGAA AAAGGTTTCT ACAAATAAAA AGGATGAGCC TCTGAAAACA 
AAGAAAACAT TCAGCATCTT GGATCCTATG CGCTGGAACC GAAATGATTC TCACTATGTT 
GACAGCAGAC CATCCAAAGT TGAGCCCGAA GCTGAGAAGC CAAACCCGGA CAGAAGGGTC 
AGCCAGGGGC AGAGAACTTT TGATAGGAGT GACAGCAGCA CAGCGAAATC CAAGAACAGC 
GAAGGGCCTG ATTCTGTGGT GTCTGTAAAT ATCATCCCCC CTCCTAATGA GCCCAATGAT 
CCTGACCCAG CAGTGACTGA TGGGGCATTG TCTAATGATG CATCTGAGGT CCTCAAAGGA 
TCCCCCACCA CTGAGCATTG CCCAGCAGAC CAGACTGAGG AAAGTGCAGA AAATGAAAGC 
CCAGAGCCTG GAGGGAGTTC AGAGGGCCCG CTGCCCTTTC TGCTTCCCCA GTCTGAGGAG 
ACTGAGCCGC GCGTTCAGGA GGCCGAGAGC GACCCCCCAA ACTGGAGGGA GCTCGTCCCC 
CCTGAAATAC TCCTCAGGCT GAAGAAAAGC GAAATCAAGC GGCAAGAGGT CATCAATGAG 
CTCTTCTTTA CAGAGCACGC TCATGTACGG ATGCTTCGTG TGTTGCATGA TGTCTTCTAC 
ATCCCCATGC AGAATGGATG GATCCCCACC TTGGAGCTGG AGAACATCTT TCCTAGCCTG 
GATGACCTTA TCGAGGCTCA TGCTGTATTC CTGGACACCT TGAAGCGTCT GCGCCTTGAG 
AATAACTCAG TGATCCAGGA AATAGGCACC ATCTTGCTGA ACCGGTTTGA CGGCACAGAG 
GGCAACTGGT TTCAGAAGAT TTCATCTCGG TTCTGCAGCC GGCAGCCATT TGCCTTGGAA 
CAGTTGAAAG CGAAACAGAA GAGAGACCCT CGATTTACGC AGTTTATTCA GGAAGCTGAG 
AGCAGCCCTC ACTGCCGGCG GTTACAACTA AAGGACATTA TACCATTTGA GATGCAAAGG 
CTGACCAAGT ACCCGTTGCT GCTGCAGAAC ATCGCAAATC TAACTGAGGA AGACGAGGAG 
AAGCAGAGAG TGCAGAGGGC AGCAGAGTCT TGCAGGCAGA TCCTAAACCA TGTTAACCAG 
GAGGTGCGCG AGATGGAGAA TAAGATGAAA CTTACAGATT ACCAAAGACG CCTGGATGTT 
TCCAACCTGA GGCAAATCAA CGACCCCTTA TTTAATGAAT TTAAGAACAT TGATATCAGC 
AAAAAGAATT TGATACATGA AGGATCTTTG ACATGGAAAG TGAGCAGGGA CAAGGCTTTT 
GATGTCCATG TCCTCCTCCT GGATGATCTC TTGATGCTGC TGCTGAAACA AGATGAGCGG 
CTGGTACTGA AGTGCCAGAG TCGGACTATT ATTCCTGCTC CAGACGGGAA GCTCATGCTG 
AGCCCAATCA TCAAGCTGAA TATGGCCATG GTCAGAGAGG TGGCTACAGA TAAGAATGCC 
TTTTTTGTGA TTTTCAACTG GGAGGATCGA GCACAGTTCT ATGAACTTGT GGCTCCAACA 
CCCGCTGAGA AGAAAAACTG GTTTTTACTG ATCAGCAACA CTACAGGCTT ATTGGAACCC 
CCAGCTTCTT CCAAACAGAG ACACAGATCT ATCACTCCTG GCACAATGCC GCAGAGCCCC 
TCCGACTTTG CAGATCAGCT GCTGAGCAGC TCAGAAAATG GAGGCTCCCT GAAAGATTCA 
GTATCCAAAG ATGAAAAGGA AGAGTCGGTG GAGGATGCAT TGGAGGGTCA GGAAGAGAAG 
GACTTCAGCG GCCTTACAGA ATACCTCCTT GCCAGCAACA TTGATCTGAA CCAAGATGCA 
GCATCTGGAG GAGGCAATGT TGCCAGCGAG GCCTTGGAAG AAGTGTCGGT GCTAAAACGC 
CTGCTAGTGC GCAATATCCG CCTCAGTGTG GATGCAGACA CGCTCTCAAC CCTGGAGACC 
TGGTCAGCGG GGGAAGAACC TGCACTGGAG GCTTCTGAAC AGGGAAGGGC CTCCAATGGG 
GATCGAGAGG CAGAGGATTC TGAAATGGAA TCTACACAAC AGGGAGAGAA TGGCAGAAAG 
CAGAGTTCTG CAGATGTTTC AGAGTCCCTG TGCGCACCCA TTGTACTGTC CAAAGAACAG 
TCGGAGGAGG TGAAGAGGCA CATTCTGTCA CTGGAGCAGA GGATCCAGCA GCTTAAGCTG 
ATCGAGGAAG ATTACAACAA CCTGCACAGA ACAATCGCCA AATTCACTGT ACCGAGGAGC 
AGCTTTACAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004916301.3
CDS	16..3207
Translation	MTHSVYLLPSCMSYCGFQKHGSQVSPGLCITYASADLSRGFPLSHSLGFCQTTPKPLVCQIRSMDPDDGHFGGLYSKGSMSIIGAEDEDFENDIEPNMVDESSYFRSIELVKEHPAYIMVFLQHVVLQFDPSPLLCFLHAEMLKNLSAKELKKVFMDFHYTFLMKGAVLHVSVPNSVSFELDRTRPEMIPEETQRKYVKDIQKHQTEAISQQLEDFRAKRVLGMTPGELELSELESYRTNDRSILEAKEKQLAEQLLARLDEMHLTIAPDEEKSSAIFCAIVAYMKHLGVKTKAADSKKSKGIFFRKKVSTNKKDEPLKTKKTFSILDPMRWNRNDSHYVDSRPSKVEPEAEKPNPDRRVSQGQRTFDRSDSSTAKSKNSEGPDSVVSVNIIPPPNEPNDPDPAVTDGALSNDASEVLKGSPTTEHCPADQTEESAENESPEPGGSSEGPLPFLLPQSEETEPRVQEAESDPPNWRELVPPEILLRLKKSEIKRQEVINELFFTEHAHVRMLRVLHDVFYIPMQNGWIPTLELENIFPSLDDLIEAHAVFLDTLKRLRLENNSVIQEIGTILLNRFDGTEGNWFQKISSRFCSRQPFALEQLKAKQKRDPRFTQFIQEAESSPHCRRLQLKDIIPFEMQRLTKYPLLLQNIANLTEEDEEKQRVQRAAESCRQILNHVNQEVREMENKMKLTDYQRRLDVSNLRQINDPLFNEFKNIDISKKNLIHEGSLTWKVSRDKAFDVHVLLLDDLLMLLLKQDERLVLKCQSRTIIPAPDGKLMLSPIIKLNMAMVREVATDKNAFFVIFNWEDRAQFYELVAPTPAEKKNWFLLISNTTGLLEPPASSKQRHRSITPGTMPQSPSDFADQLLSSSENGGSLKDSVSKDEKEESVEDALEGQEEKDFSGLTEYLLASNIDLNQDAASGGGNVASEALEEVSVLKRLLVRNIRLSVDADTLSTLETWSAGEEPALEASEQGRASNGDREAEDSEMESTQQGENGRKQSSADVSESLCAPIVLSKEQSEEVKRHILSLEQRIQQLKLIEEDYNNLHRTIAKFTVPRSSFT

Target ORF information:

RefSeq Version	XM_004916244.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho guanine nucleotide exchange factor 1 (arhgef1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004916244.4

ORF Insert Sequence:

ATGACTCACT CTGTCTACCT CTTACCCTCC TGCATGAGTT ATTGTGGATT CCAAAAGCAC 
GGCTCACAGG TTTCTCCTGG TCTGTGCATC ACTTATGCAT CAGCCGACCT GAGCAGGGGA 
TTCCCATTGT CTCACTCACT GGGTTTTTGC CAGACTACTC CCAAACCCCT CGTCTGCCAG 
ATAAGAAGCA TGGATCCGGA CGATGGCCAT TTTGGGGGTT TGTACTCCAA GGGGTCCATG 
TCGATCATTG GAGCAGAGGA TGAGGATTTT GAGAATGACA TTGAACCGAA TATGGTTGAT 
GAGAGCAGTT ACTTCCGAAG CATTGAGCTA GTGAAAGAAC ACCCGGCCTA CATTATGGTG 
TTCTTGCAGC ACGTGGTTCT TCAGTTTGAC CCCAGCCCAC TGCTCTGTTT CCTGCATGCG 
GAGATGCTAA AGAACCTTTC AGCCAAAGAG CTTAAGAAAG TCTTCATGGA TTTTCACTAC 
ACATTTCTGA TGAAAGGAGC GGTACTGCAT GTCAGTGTGC CAAACAGCGT ATCCTTTGAA 
CTGGATCGGA CAAGGCCTGA GATGATCCCA GAAGAAACCC AGAGAAAATA TGTCAAAGAC 
ATTCAGAAGC ACCAAACTGA GGCCATTTCC CAACAGCTGG AGGACTTTAG GGCCAAGCGA 
GTACTTGGTA TGACCCCTGG GGAACTGGAG CTGAGCGAGC TTGAATCCTA TCGAACCAAC 
GACCGCAGTA TCCTGGAAGC CAAAGAGAAG CAGTTGGCAG AGCAGCTCCT GGCACGGCTG 
GATGAGATGC ACCTAACTAT TGCTCCAGAC GAGGAAAAAA GCTCTGCCAT CTTTTGTGCC 
ATTGTAGCCT ACATGAAGCA CCTGGGGGTG AAGACCAAGG CGGCGGATAG CAAAAAATCT 
AAAGGAATCT TCTTCAGGAA AAAGGTTTCT ACAAATAAAA AGGATGAGCC TCTGAAAACA 
AAGAAAACAT TCAGCATCTT GGATCCTATG CGCTGGAACC GAAATGATTC TCACTATGTT 
GACAGCAGAC CATCCAAAGT TGAGCCCGAA GCTGAGAAGC CAAACCCGGA CAGAAGGGTC 
AGCCAGGGGC AGAGAACTTT TGATAGGAGT GACAGCAGCA CAGCGAAATC CAAGAACAGC 
GAAGGGCCTG ATTCTGTGGT GTCTGTAAAT ATCATCCCCC CTCCTAATGA GCCCAATGAT 
CCTGACCCAG CAGTGACTGA TGGGGCATTG TCTAATGATG CATCTGAGGT CCTCAAAGGA 
TCCCCCACCA CTGAGCATTG CCCAGCAGAC CAGACTGAGG AAAGTGCAGA AAATGAAAGC 
CCAGAGCCTG GAGGGAGTTC AGAGGGCCCG CTGCCCTTTC TGCTTCCCCA GTCTGAGGAG 
ACTGAGCCGC GCGTTCAGGA GGCCGAGAGC GACCCCCCAA ACTGGAGGGA GCTCGTCCCC 
CCTGAAATAC TCCTCAGGCT GAAGAAAAGC GAAATCAAGC GGCAAGAGGT CATCAATGAG 
CTCTTCTTTA CAGAGCACGC TCATGTACGG ATGCTTCGTG TGTTGCATGA TGTCTTCTAC 
ATCCCCATGC AGAATGGATG GATCCCCACC TTGGAGCTGG AGAACATCTT TCCTAGCCTG 
GATGACCTTA TCGAGGCTCA TGCTGTATTC CTGGACACCT TGAAGCGTCT GCGCCTTGAG 
AATAACTCAG TGATCCAGGA AATAGGCACC ATCTTGCTGA ACCGGTTTGA CGGCACAGAG 
GGCAACTGGT TTCAGAAGAT TTCATCTCGG TTCTGCAGCC GGCAGCCATT TGCCTTGGAA 
CAGTTGAAAG CGAAACAGAA GAGAGACCCT CGATTTACGC AGTTTATTCA GGAAGCTGAG 
AGCAGCCCTC ACTGCCGGCG GTTACAACTA AAGGACATTA TACCATTTGA GATGCAAAGG 
CTGACCAAGT ACCCGTTGCT GCTGCAGAAC ATCGCAAATC TAACTGAGGA AGACGAGGAG 
AAGCAGAGAG TGCAGAGGGC AGCAGAGTCT TGCAGGCAGA TCCTAAACCA TGTTAACCAG 
GAGGTGCGCG AGATGGAGAA TAAGATGAAA CTTACAGATT ACCAAAGACG CCTGGATGTT 
TCCAACCTGA GGCAAATCAA CGACCCCTTA TTTAATGAAT TTAAGAACAT TGATATCAGC 
AAAAAGAATT TGATACATGA AGGATCTTTG ACATGGAAAG TGAGCAGGGA CAAGGCTTTT 
GATGTCCATG TCCTCCTCCT GGATGATCTC TTGATGCTGC TGCTGAAACA AGATGAGCGG 
CTGGTACTGA AGTGCCAGAG TCGGACTATT ATTCCTGCTC CAGACGGGAA GCTCATGCTG 
AGCCCAATCA TCAAGCTGAA TATGGCCATG GTCAGAGAGG TGGCTACAGA TAAGAATGCC 
TTTTTTGTGA TTTTCAACTG GGAGGATCGA GCACAGTTCT ATGAACTTGT GGCTCCAACA 
CCCGCTGAGA AGAAAAACTG GTTTTTACTG ATCAGCAACA CTACAGGCTT ATTGGAACCC 
CCAGCTTCTT CCAAACAGAG ACACAGATCT ATCACTCCTG GCACAATGCC GCAGAGCCCC 
TCCGACTTTG CAGATCAGCT GCTGAGCAGC TCAGAAAATG GAGGCTCCCT GAAAGATTCA 
GTATCCAAAG ATGAAAAGGA AGAGTCGGTG GAGGATGCAT TGGAGGGTCA GGAAGAGAAG 
GACTTCAGCG GCCTTACAGA ATACCTCCTT GCCAGCAACA TTGATCTGAA CCAAGATGCA 
GCATCTGGAG GAGGCAATGT TGCCAGCGAG GCCTTGGAAG AAGTGTCGGT GCTAAAACGC 
CTGCTAGTGC GCAATATCCG CCTCAGTGTG GATGCAGACA CGCTCTCAAC CCTGGAGACC 
TGGTCAGCGG GGGAAGAACC TGCACTGGAG GCTTCTGAAC AGGGAAGGGC CTCCAATGGG 
GATCGAGAGG CAGAGGATTC TGAAATGGAA TCTACACAAC AGGGAGAGAA TGGCAGAAAG 
CAGAGTTCTG CAGATGTTTC AGAGTCCCTG TGCGCACCCA TTGTACTGTC CAAAGAACAG 
TCGGAGGAGG TGAAGAGGCA CATTCTGTCA CTGGAGCAGA GGATCCAGCA GCTTAAGCTG 
ATCGAGGAAG ATTACAACAA CCTGCACAGA ACAATCGCCA AATTCACTGT ACCGAGGAGC 
AGCTTTACAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgef1 ( XM_004916245.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004916302.3 Xenopus tropicalis Rho guanine nucleotide exchange factor 1 (arhgef1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OXa62578
Clone ID Related Accession (Same CDS sequence)	XM_004916245.4
Accession Version	XM_004916245.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3156bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho guanine nucleotide exchange factor 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004916245.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTCACT CTGTCTACCT CTTACCCTCC TGCATGAGTT ATTGTGGATT CCAAAAGCAC 
GGCTCACAGG TTTCTCCTGG TCTGTGCATC ACTTATGCAT CAGCCGACCT GAGCAGGGGA 
TTCCCATTGT CTCACTCACT GGGTTTTTGC CAGACTACTC CCAAACCCCT CGTCTGCCAG 
ATAAGAAGCA TGGATCCGGA CGATGGCCAT TTTGGGGGTT TGTACTCCAA GGGGTCCATG 
TCGATCATTG GAGCAGAGGA TGAGGATTTT GAGAATGACA TTGAACCGAA TATGGTTGAT 
GAGAGCAGTT ACTTCCGAAG CATTGAGCTA GTGAAAGAAC ACCCGGCCTA CATTATGGTG 
TTCTTGCAGC ACGTGGTTCT TCAGTTTGAC CCCAGCCCAC TGCTCTGTTT CCTGCATGCG 
GAGATGCTAA AGAACCTTTC AGCCAAAGAG CTTAAGAAAG TCTTCATGGA TTTTCACTAC 
ACATTTCTGA TGAAAGGAGC GGTACTGCAT GTCAGTGTGC CAAACAGCGT ATCCTTTGAA 
CTGGATCGGA CAAGGCCTGA GATGATCCCA GAAGAAACCC AGAGAAAATA TGTCAAAGAC 
ATTCAGAAGC ACCAAACTGA GGCCATTTCC CAACAGCTGG AGGACTTTAG GGCCAAGCGA 
GTACTTGGTA TGACCCCTGG GGAACTGGAG CTGAGCGAGC TTGAATCCTA TCGAACCAAC 
GACCGCAGTA TCCTGGAAGC CAAAGAGAAG CAGTTGGCAG AGCAGCTCCT GGCACGGCTG 
GATGAGATGC ACCTAACTAT TGCTCCAGAC GAGGAAAAAA GCTCTGCCAT CTTTTGTGCC 
ATTGTAGCCT ACATGAAGCA CCTGGGGGTG AAGACCAAGG CGGCGGATAG CAAAAAATCT 
AAAGGAATCT TCTTCAGGAA AAAGGTTTCT ACAAATAAAA AGGATGAGCC TCTGAAAACA 
AAGAAAACAT TCAGCATCTT GGATCCTATG CGCTGGAACC GAAATGATTC TCACTCTGAG 
AAGCCAAACC CGGACAGAAG GGTCAGCCAG GGGCAGAGAA CTTTTGATAG GAGTGACAGC 
AGCACAGCGA AATCCAAGAA CAGCGAAGGG CCTGATTCTG TGGTGTCTGT AAATATCATC 
CCCCCTCCTA ATGAGCCCAA TGATCCTGAC CCAGCAGTGA CTGATGGGGC ATTGTCTAAT 
GATGCATCTG AGGTCCTCAA AGGATCCCCC ACCACTGAGC ATTGCCCAGC AGACCAGACT 
GAGGAAAGTG CAGAAAATGA AAGCCCAGAG CCTGGAGGGA GTTCAGAGGG CCCGCTGCCC 
TTTCTGCTTC CCCAGTCTGA GGAGACTGAG CCGCGCGTTC AGGAGGCCGA GAGCGACCCC 
CCAAACTGGA GGGAGCTCGT CCCCCCTGAA ATACTCCTCA GGCTGAAGAA AAGCGAAATC 
AAGCGGCAAG AGGTCATCAA TGAGCTCTTC TTTACAGAGC ACGCTCATGT ACGGATGCTT 
CGTGTGTTGC ATGATGTCTT CTACATCCCC ATGCAGAATG GATGGATCCC CACCTTGGAG 
CTGGAGAACA TCTTTCCTAG CCTGGATGAC CTTATCGAGG CTCATGCTGT ATTCCTGGAC 
ACCTTGAAGC GTCTGCGCCT TGAGAATAAC TCAGTGATCC AGGAAATAGG CACCATCTTG 
CTGAACCGGT TTGACGGCAC AGAGGGCAAC TGGTTTCAGA AGATTTCATC TCGGTTCTGC 
AGCCGGCAGC CATTTGCCTT GGAACAGTTG AAAGCGAAAC AGAAGAGAGA CCCTCGATTT 
ACGCAGTTTA TTCAGGAAGC TGAGAGCAGC CCTCACTGCC GGCGGTTACA ACTAAAGGAC 
ATTATACCAT TTGAGATGCA AAGGCTGACC AAGTACCCGT TGCTGCTGCA GAACATCGCA 
AATCTAACTG AGGAAGACGA GGAGAAGCAG AGAGTGCAGA GGGCAGCAGA GTCTTGCAGG 
CAGATCCTAA ACCATGTTAA CCAGGAGGTG CGCGAGATGG AGAATAAGAT GAAACTTACA 
GATTACCAAA GACGCCTGGA TGTTTCCAAC CTGAGGCAAA TCAACGACCC CTTATTTAAT 
GAATTTAAGA ACATTGATAT CAGCAAAAAG AATTTGATAC ATGAAGGATC TTTGACATGG 
AAAGTGAGCA GGGACAAGGC TTTTGATGTC CATGTCCTCC TCCTGGATGA TCTCTTGATG 
CTGCTGCTGA AACAAGATGA GCGGCTGGTA CTGAAGTGCC AGAGTCGGAC TATTATTCCT 
GCTCCAGACG GGAAGCTCAT GCTGAGCCCA ATCATCAAGC TGAATATGGC CATGGTCAGA 
GAGGTGGCTA CAGATAAGAA TGCCTTTTTT GTGATTTTCA ACTGGGAGGA TCGAGCACAG 
TTCTATGAAC TTGTGGCTCC AACACCCGCT GAGAAGAAAA ACTGGTTTTT ACTGATCAGC 
AACACTACAG GCTTATTGGA ACCCCCAGCT TCTTCCAAAC AGAGACACAG ATCTATCACT 
CCTGGCACAA TGCCGCAGAG CCCCTCCGAC TTTGCAGATC AGCTGCTGAG CAGCTCAGAA 
AATGGAGGCT CCCTGAAAGA TTCAGTATCC AAAGATGAAA AGGAAGAGTC GGTGGAGGAT 
GCATTGGAGG GTCAGGAAGA GAAGGACTTC AGCGGCCTTA CAGAATACCT CCTTGCCAGC 
AACATTGATC TGAACCAAGA TGCAGCATCT GGAGGAGGCA ATGTTGCCAG CGAGGCCTTG 
GAAGAAGTGT CGGTGCTAAA ACGCCTGCTA GTGCGCAATA TCCGCCTCAG TGTGGATGCA 
GACACGCTCT CAACCCTGGA GACCTGGTCA GCGGGGGAAG AACCTGCACT GGAGGCTTCT 
GAACAGGGAA GGGCCTCCAA TGGGGATCGA GAGGCAGAGG ATTCTGAAAT GGAATCTACA 
CAACAGGGAG AGAATGGCAG AAAGCAGAGT TCTGCAGATG TTTCAGAGTC CCTGTGCGCA 
CCCATTGTAC TGTCCAAAGA ACAGTCGGAG GAGGTGAAGA GGCACATTCT GTCACTGGAG 
CAGAGGATCC AGCAGCTTAA GCTGATCGAG GAAGATTACA ACAACCTGCA CAGAACAATC 
GCCAAATTCA CTGTACCGAG GAGCAGCTTT ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004916302.3
CDS	16..3171
Translation	MTHSVYLLPSCMSYCGFQKHGSQVSPGLCITYASADLSRGFPLSHSLGFCQTTPKPLVCQIRSMDPDDGHFGGLYSKGSMSIIGAEDEDFENDIEPNMVDESSYFRSIELVKEHPAYIMVFLQHVVLQFDPSPLLCFLHAEMLKNLSAKELKKVFMDFHYTFLMKGAVLHVSVPNSVSFELDRTRPEMIPEETQRKYVKDIQKHQTEAISQQLEDFRAKRVLGMTPGELELSELESYRTNDRSILEAKEKQLAEQLLARLDEMHLTIAPDEEKSSAIFCAIVAYMKHLGVKTKAADSKKSKGIFFRKKVSTNKKDEPLKTKKTFSILDPMRWNRNDSHSEKPNPDRRVSQGQRTFDRSDSSTAKSKNSEGPDSVVSVNIIPPPNEPNDPDPAVTDGALSNDASEVLKGSPTTEHCPADQTEESAENESPEPGGSSEGPLPFLLPQSEETEPRVQEAESDPPNWRELVPPEILLRLKKSEIKRQEVINELFFTEHAHVRMLRVLHDVFYIPMQNGWIPTLELENIFPSLDDLIEAHAVFLDTLKRLRLENNSVIQEIGTILLNRFDGTEGNWFQKISSRFCSRQPFALEQLKAKQKRDPRFTQFIQEAESSPHCRRLQLKDIIPFEMQRLTKYPLLLQNIANLTEEDEEKQRVQRAAESCRQILNHVNQEVREMENKMKLTDYQRRLDVSNLRQINDPLFNEFKNIDISKKNLIHEGSLTWKVSRDKAFDVHVLLLDDLLMLLLKQDERLVLKCQSRTIIPAPDGKLMLSPIIKLNMAMVREVATDKNAFFVIFNWEDRAQFYELVAPTPAEKKNWFLLISNTTGLLEPPASSKQRHRSITPGTMPQSPSDFADQLLSSSENGGSLKDSVSKDEKEESVEDALEGQEEKDFSGLTEYLLASNIDLNQDAASGGGNVASEALEEVSVLKRLLVRNIRLSVDADTLSTLETWSAGEEPALEASEQGRASNGDREAEDSEMESTQQGENGRKQSSADVSESLCAPIVLSKEQSEEVKRHILSLEQRIQQLKLIEEDYNNLHRTIAKFTVPRSSFT

Target ORF information:

RefSeq Version	XM_004916245.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho guanine nucleotide exchange factor 1 (arhgef1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004916245.4

ORF Insert Sequence:

ATGACTCACT CTGTCTACCT CTTACCCTCC TGCATGAGTT ATTGTGGATT CCAAAAGCAC 
GGCTCACAGG TTTCTCCTGG TCTGTGCATC ACTTATGCAT CAGCCGACCT GAGCAGGGGA 
TTCCCATTGT CTCACTCACT GGGTTTTTGC CAGACTACTC CCAAACCCCT CGTCTGCCAG 
ATAAGAAGCA TGGATCCGGA CGATGGCCAT TTTGGGGGTT TGTACTCCAA GGGGTCCATG 
TCGATCATTG GAGCAGAGGA TGAGGATTTT GAGAATGACA TTGAACCGAA TATGGTTGAT 
GAGAGCAGTT ACTTCCGAAG CATTGAGCTA GTGAAAGAAC ACCCGGCCTA CATTATGGTG 
TTCTTGCAGC ACGTGGTTCT TCAGTTTGAC CCCAGCCCAC TGCTCTGTTT CCTGCATGCG 
GAGATGCTAA AGAACCTTTC AGCCAAAGAG CTTAAGAAAG TCTTCATGGA TTTTCACTAC 
ACATTTCTGA TGAAAGGAGC GGTACTGCAT GTCAGTGTGC CAAACAGCGT ATCCTTTGAA 
CTGGATCGGA CAAGGCCTGA GATGATCCCA GAAGAAACCC AGAGAAAATA TGTCAAAGAC 
ATTCAGAAGC ACCAAACTGA GGCCATTTCC CAACAGCTGG AGGACTTTAG GGCCAAGCGA 
GTACTTGGTA TGACCCCTGG GGAACTGGAG CTGAGCGAGC TTGAATCCTA TCGAACCAAC 
GACCGCAGTA TCCTGGAAGC CAAAGAGAAG CAGTTGGCAG AGCAGCTCCT GGCACGGCTG 
GATGAGATGC ACCTAACTAT TGCTCCAGAC GAGGAAAAAA GCTCTGCCAT CTTTTGTGCC 
ATTGTAGCCT ACATGAAGCA CCTGGGGGTG AAGACCAAGG CGGCGGATAG CAAAAAATCT 
AAAGGAATCT TCTTCAGGAA AAAGGTTTCT ACAAATAAAA AGGATGAGCC TCTGAAAACA 
AAGAAAACAT TCAGCATCTT GGATCCTATG CGCTGGAACC GAAATGATTC TCACTCTGAG 
AAGCCAAACC CGGACAGAAG GGTCAGCCAG GGGCAGAGAA CTTTTGATAG GAGTGACAGC 
AGCACAGCGA AATCCAAGAA CAGCGAAGGG CCTGATTCTG TGGTGTCTGT AAATATCATC 
CCCCCTCCTA ATGAGCCCAA TGATCCTGAC CCAGCAGTGA CTGATGGGGC ATTGTCTAAT 
GATGCATCTG AGGTCCTCAA AGGATCCCCC ACCACTGAGC ATTGCCCAGC AGACCAGACT 
GAGGAAAGTG CAGAAAATGA AAGCCCAGAG CCTGGAGGGA GTTCAGAGGG CCCGCTGCCC 
TTTCTGCTTC CCCAGTCTGA GGAGACTGAG CCGCGCGTTC AGGAGGCCGA GAGCGACCCC 
CCAAACTGGA GGGAGCTCGT CCCCCCTGAA ATACTCCTCA GGCTGAAGAA AAGCGAAATC 
AAGCGGCAAG AGGTCATCAA TGAGCTCTTC TTTACAGAGC ACGCTCATGT ACGGATGCTT 
CGTGTGTTGC ATGATGTCTT CTACATCCCC ATGCAGAATG GATGGATCCC CACCTTGGAG 
CTGGAGAACA TCTTTCCTAG CCTGGATGAC CTTATCGAGG CTCATGCTGT ATTCCTGGAC 
ACCTTGAAGC GTCTGCGCCT TGAGAATAAC TCAGTGATCC AGGAAATAGG CACCATCTTG 
CTGAACCGGT TTGACGGCAC AGAGGGCAAC TGGTTTCAGA AGATTTCATC TCGGTTCTGC 
AGCCGGCAGC CATTTGCCTT GGAACAGTTG AAAGCGAAAC AGAAGAGAGA CCCTCGATTT 
ACGCAGTTTA TTCAGGAAGC TGAGAGCAGC CCTCACTGCC GGCGGTTACA ACTAAAGGAC 
ATTATACCAT TTGAGATGCA AAGGCTGACC AAGTACCCGT TGCTGCTGCA GAACATCGCA 
AATCTAACTG AGGAAGACGA GGAGAAGCAG AGAGTGCAGA GGGCAGCAGA GTCTTGCAGG 
CAGATCCTAA ACCATGTTAA CCAGGAGGTG CGCGAGATGG AGAATAAGAT GAAACTTACA 
GATTACCAAA GACGCCTGGA TGTTTCCAAC CTGAGGCAAA TCAACGACCC CTTATTTAAT 
GAATTTAAGA ACATTGATAT CAGCAAAAAG AATTTGATAC ATGAAGGATC TTTGACATGG 
AAAGTGAGCA GGGACAAGGC TTTTGATGTC CATGTCCTCC TCCTGGATGA TCTCTTGATG 
CTGCTGCTGA AACAAGATGA GCGGCTGGTA CTGAAGTGCC AGAGTCGGAC TATTATTCCT 
GCTCCAGACG GGAAGCTCAT GCTGAGCCCA ATCATCAAGC TGAATATGGC CATGGTCAGA 
GAGGTGGCTA CAGATAAGAA TGCCTTTTTT GTGATTTTCA ACTGGGAGGA TCGAGCACAG 
TTCTATGAAC TTGTGGCTCC AACACCCGCT GAGAAGAAAA ACTGGTTTTT ACTGATCAGC 
AACACTACAG GCTTATTGGA ACCCCCAGCT TCTTCCAAAC AGAGACACAG ATCTATCACT 
CCTGGCACAA TGCCGCAGAG CCCCTCCGAC TTTGCAGATC AGCTGCTGAG CAGCTCAGAA 
AATGGAGGCT CCCTGAAAGA TTCAGTATCC AAAGATGAAA AGGAAGAGTC GGTGGAGGAT 
GCATTGGAGG GTCAGGAAGA GAAGGACTTC AGCGGCCTTA CAGAATACCT CCTTGCCAGC 
AACATTGATC TGAACCAAGA TGCAGCATCT GGAGGAGGCA ATGTTGCCAG CGAGGCCTTG 
GAAGAAGTGT CGGTGCTAAA ACGCCTGCTA GTGCGCAATA TCCGCCTCAG TGTGGATGCA 
GACACGCTCT CAACCCTGGA GACCTGGTCA GCGGGGGAAG AACCTGCACT GGAGGCTTCT 
GAACAGGGAA GGGCCTCCAA TGGGGATCGA GAGGCAGAGG ATTCTGAAAT GGAATCTACA 
CAACAGGGAG AGAATGGCAG AAAGCAGAGT TCTGCAGATG TTTCAGAGTC CCTGTGCGCA 
CCCATTGTAC TGTCCAAAGA ACAGTCGGAG GAGGTGAAGA GGCACATTCT GTCACTGGAG 
CAGAGGATCC AGCAGCTTAA GCTGATCGAG GAAGATTACA ACAACCTGCA CAGAACAATC 
GCCAAATTCA CTGTACCGAG GAGCAGCTTT ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgef1 ( XM_012967446.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012822900.2 Xenopus tropicalis Rho guanine nucleotide exchange factor 1 (arhgef1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OXa62579
Clone ID Related Accession (Same CDS sequence)	XM_012967446.3
Accession Version	XM_012967446.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3198bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho guanine nucleotide exchange factor 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012967446.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCCGG ACGATGGCCA TTTTGGGGGT TTGTACTCCA AGGGGTCCAT GTCGATCATT 
GGAGCAGAGG ATGAGGATTT TGAGAATGAC ATTGAACCGA ATATGGTTGA TGAGAGCAGT 
TACTTCCGAA GCATTGAGCT AGTGAAAGAA CACCCGGCCT ACATTATGGT GTTCTTGCAG 
CACGTGGTTC TTCAGTTTGA CCCCAGCCCA CTGCTCTGTT TCCTGCATGC GGAGATGCTA 
AAGAACCTTT CAGCCAAAGA GCTTAAGAAA GTCTTCATGG ATTTTCACTA CACATTTCTG 
ATGAAAGGAG CGGTACTGCA TGTCAGTGTG CCAAACAGCG TATCCTTTGA ACTGGATCGG 
ACAAGGCCTG AGATGATCCC AGAAGAAACC CAGAGAAAAT ATGTCAAAGA CATTCAGAAG 
CACCAAACTG AGGCCATTTC CCAACAGCTG GAGGACTTTA GGGCCAAGCG AGTACTTGGT 
ATGACCCCTG GGGAACTGGA GCTGAGCGAG CTTGAATCCT ATCGAACCAA CGACCGCAGT 
ATCCTGGAAG CCAAAGAGAA GCAGTTGGCA GAGCAGCTCC TGGCACGGCT GGATGAGATG 
CACCTAACTA TTGCTCCAGA CGAGGAAAAA AGCTCTGCCA TCTTTTGTGC CATTGTAGCC 
TACATGAAGC ACCTGGGGGT GAAGACCAAG GCGGCGGATA GCAAAAAATC TAAAGGAATC 
TTCTTCAGGA AAAAGGTTTC TACAAATAAA AAGGATGAGC CTCTGAAAAC AAAGAAAACA 
TTCAGCATCT TGGATCCTAT GCGCTGGAAC CGAAATGATT CTCACTATGT TGACAGCAGA 
CCATCCAAAG TTGAGCCCGA AGCTGAGAAG CCAAACCCGG ACAGAAGGGT CAGCCAGGGG 
CAGAGAACTT TTGATAGGAG TGACAGCAGC ACAGCGAAAT CCAAGAACAG CGAAGGGCCT 
GATTCTGTGG TGTCTGTAAA TATCATCCCC CCTCCTAATG AGCCCAATGA TCCTGACCCA 
GCAGTGACTG ATGGGGCATT GTCTAATGAT GCATCTGAGG TCCTCAAAGG ATCCCCCACC 
ACTGAGCATT GCCCAGCAGA CCAGACTGAG GAAAGTGCAG AAAATGAAAG ACGAAAGAAA 
AGGCTCTCTG GTAAGCTGGG CCGCTCGGAG AGCCTACGAG TTTACGAGCG CAAGCGTTCC 
CAGCGCGGCT CCTCCAAAGC CAAGCAGACA CGTTCCCGTA GCGACGTTGA CCTGGAGGCA 
GCTGCCCGAG CCAACGACTT GCAGGGCACC ACTGGCAAGC TTGGGCCAAA GCCTGGCAAC 
TACACCCCAG AGCCTGGAGG GAGTTCAGAG GGCCCGCTGC CCTTTCTGCT TCCCCAGTCT 
GAGGAGACTG AGCCGCGCGT TCAGGAGGCC GAGAGCGACC CCCCAAACTG GAGGGAGCTC 
GTCCCCCCTG AAATACTCCT CAGGCTGAAG AAAAGCGAAA TCAAGCGGCA AGAGGTCATC 
AATGAGCTCT TCTTTACAGA GCACGCTCAT GTACGGATGC TTCGTGTGTT GCATGATGTC 
TTCTACATCC CCATGCAGAA TGGATGGATC CCCACCTTGG AGCTGGAGAA CATCTTTCCT 
AGCCTGGATG ACCTTATCGA GGCTCATGCT GTATTCCTGG ACACCTTGAA GCGTCTGCGC 
CTTGAGAATA ACTCAGTGAT CCAGGAAATA GGCACCATCT TGCTGAACCG GTTTGACGGC 
ACAGAGGGCA ACTGGTTTCA GAAGATTTCA TCTCGGTTCT GCAGCCGGCA GCCATTTGCC 
TTGGAACAGT TGAAAGCGAA ACAGAAGAGA GACCCTCGAT TTACGCAGTT TATTCAGGAA 
GCTGAGAGCA GCCCTCACTG CCGGCGGTTA CAACTAAAGG ACATTATACC ATTTGAGATG 
CAAAGGCTGA CCAAGTACCC GTTGCTGCTG CAGAACATCG CAAATCTAAC TGAGGAAGAC 
GAGGAGAAGC AGAGAGTGCA GAGGGCAGCA GAGTCTTGCA GGCAGATCCT AAACCATGTT 
AACCAGGAGG TGCGCGAGAT GGAGAATAAG ATGAAACTTA CAGATTACCA AAGACGCCTG 
GATGTTTCCA ACCTGAGGCA AATCAACGAC CCCTTATTTA ATGAATTTAA GAACATTGAT 
ATCAGCAAAA AGAATTTGAT ACATGAAGGA TCTTTGACAT GGAAAGTGAG CAGGGACAAG 
GCTTTTGATG TCCATGTCCT CCTCCTGGAT GATCTCTTGA TGCTGCTGCT GAAACAAGAT 
GAGCGGCTGG TACTGAAGTG CCAGAGTCGG ACTATTATTC CTGCTCCAGA CGGGAAGCTC 
ATGCTGAGCC CAATCATCAA GCTGAATATG GCCATGGTCA GAGAGGTGGC TACAGATAAG 
AATGCCTTTT TTGTGATTTT CAACTGGGAG GATCGAGCAC AGTTCTATGA ACTTGTGGCT 
CCAACACCCG CTGAGAAGAA AAACTGGTTT TTACTGATCA GCAACACTAC AGGCTTATTG 
GAACCCCCAG CTTCTTCCAA ACAGAGACAC AGATCTATCA CTCCTGGCAC AATGCCGCAG 
AGCCCCTCCG ACTTTGCAGA TCAGCTGCTG AGCAGCTCAG AAAATGGAGG CTCCCTGAAA 
GATTCAGTAT CCAAAGATGA AAAGGAAGAG TCGGTGGAGG ATGCATTGGA GGGTCAGGAA 
GAGAAGGACT TCAGCGGCCT TACAGAATAC CTCCTTGCCA GCAACATTGA TCTGAACCAA 
GATGCAGCAT CTGGAGGAGG CAATGTTGCC AGCGAGGCCT TGGAAGAAGT GTCGGTGCTA 
AAACGCCTGC TAGTGCGCAA TATCCGCCTC AGTGTGGATG CAGACACGCT CTCAACCCTG 
GAGACCTGGT CAGCGGGGGA AGAACCTGCA CTGGAGGCTT CTGAACAGGG AAGGGCCTCC 
AATGGGGATC GAGAGGCAGA GGATTCTGAA ATGGAATCTA CACAACAGGG AGAGAATGGC 
AGAAAGCAGA GTTCTGCAGA TGTTTCAGAG TCCCTGTGCG CACCCATTGT ACTGTCCAAA 
GAACAGTCGG AGGAGGTGAA GAGGCACATT CTGTCACTGG AGCAGAGGAT CCAGCAGCTT 
AAGCTGATCG AGGAAGATTA CAACAACCTG CACAGAACAA TCGCCAAATT CACTGTACCG 
AGGAGCAGCT TTACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012822900.2
CDS	330..3527
Translation	MDPDDGHFGGLYSKGSMSIIGAEDEDFENDIEPNMVDESSYFRSIELVKEHPAYIMVFLQHVVLQFDPSPLLCFLHAEMLKNLSAKELKKVFMDFHYTFLMKGAVLHVSVPNSVSFELDRTRPEMIPEETQRKYVKDIQKHQTEAISQQLEDFRAKRVLGMTPGELELSELESYRTNDRSILEAKEKQLAEQLLARLDEMHLTIAPDEEKSSAIFCAIVAYMKHLGVKTKAADSKKSKGIFFRKKVSTNKKDEPLKTKKTFSILDPMRWNRNDSHYVDSRPSKVEPEAEKPNPDRRVSQGQRTFDRSDSSTAKSKNSEGPDSVVSVNIIPPPNEPNDPDPAVTDGALSNDASEVLKGSPTTEHCPADQTEESAENERRKKRLSGKLGRSESLRVYERKRSQRGSSKAKQTRSRSDVDLEAAARANDLQGTTGKLGPKPGNYTPEPGGSSEGPLPFLLPQSEETEPRVQEAESDPPNWRELVPPEILLRLKKSEIKRQEVINELFFTEHAHVRMLRVLHDVFYIPMQNGWIPTLELENIFPSLDDLIEAHAVFLDTLKRLRLENNSVIQEIGTILLNRFDGTEGNWFQKISSRFCSRQPFALEQLKAKQKRDPRFTQFIQEAESSPHCRRLQLKDIIPFEMQRLTKYPLLLQNIANLTEEDEEKQRVQRAAESCRQILNHVNQEVREMENKMKLTDYQRRLDVSNLRQINDPLFNEFKNIDISKKNLIHEGSLTWKVSRDKAFDVHVLLLDDLLMLLLKQDERLVLKCQSRTIIPAPDGKLMLSPIIKLNMAMVREVATDKNAFFVIFNWEDRAQFYELVAPTPAEKKNWFLLISNTTGLLEPPASSKQRHRSITPGTMPQSPSDFADQLLSSSENGGSLKDSVSKDEKEESVEDALEGQEEKDFSGLTEYLLASNIDLNQDAASGGGNVASEALEEVSVLKRLLVRNIRLSVDADTLSTLETWSAGEEPALEASEQGRASNGDREAEDSEMESTQQGENGRKQSSADVSESLCAPIVLSKEQSEEVKRHILSLEQRIQQLKLIEEDYNNLHRTIAKFTVPRSSFT

Target ORF information:

RefSeq Version	XM_012967446.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho guanine nucleotide exchange factor 1 (arhgef1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012967446.3

ORF Insert Sequence:

ATGGATCCGG ACGATGGCCA TTTTGGGGGT TTGTACTCCA AGGGGTCCAT GTCGATCATT 
GGAGCAGAGG ATGAGGATTT TGAGAATGAC ATTGAACCGA ATATGGTTGA TGAGAGCAGT 
TACTTCCGAA GCATTGAGCT AGTGAAAGAA CACCCGGCCT ACATTATGGT GTTCTTGCAG 
CACGTGGTTC TTCAGTTTGA CCCCAGCCCA CTGCTCTGTT TCCTGCATGC GGAGATGCTA 
AAGAACCTTT CAGCCAAAGA GCTTAAGAAA GTCTTCATGG ATTTTCACTA CACATTTCTG 
ATGAAAGGAG CGGTACTGCA TGTCAGTGTG CCAAACAGCG TATCCTTTGA ACTGGATCGG 
ACAAGGCCTG AGATGATCCC AGAAGAAACC CAGAGAAAAT ATGTCAAAGA CATTCAGAAG 
CACCAAACTG AGGCCATTTC CCAACAGCTG GAGGACTTTA GGGCCAAGCG AGTACTTGGT 
ATGACCCCTG GGGAACTGGA GCTGAGCGAG CTTGAATCCT ATCGAACCAA CGACCGCAGT 
ATCCTGGAAG CCAAAGAGAA GCAGTTGGCA GAGCAGCTCC TGGCACGGCT GGATGAGATG 
CACCTAACTA TTGCTCCAGA CGAGGAAAAA AGCTCTGCCA TCTTTTGTGC CATTGTAGCC 
TACATGAAGC ACCTGGGGGT GAAGACCAAG GCGGCGGATA GCAAAAAATC TAAAGGAATC 
TTCTTCAGGA AAAAGGTTTC TACAAATAAA AAGGATGAGC CTCTGAAAAC AAAGAAAACA 
TTCAGCATCT TGGATCCTAT GCGCTGGAAC CGAAATGATT CTCACTATGT TGACAGCAGA 
CCATCCAAAG TTGAGCCCGA AGCTGAGAAG CCAAACCCGG ACAGAAGGGT CAGCCAGGGG 
CAGAGAACTT TTGATAGGAG TGACAGCAGC ACAGCGAAAT CCAAGAACAG CGAAGGGCCT 
GATTCTGTGG TGTCTGTAAA TATCATCCCC CCTCCTAATG AGCCCAATGA TCCTGACCCA 
GCAGTGACTG ATGGGGCATT GTCTAATGAT GCATCTGAGG TCCTCAAAGG ATCCCCCACC 
ACTGAGCATT GCCCAGCAGA CCAGACTGAG GAAAGTGCAG AAAATGAAAG ACGAAAGAAA 
AGGCTCTCTG GTAAGCTGGG CCGCTCGGAG AGCCTACGAG TTTACGAGCG CAAGCGTTCC 
CAGCGCGGCT CCTCCAAAGC CAAGCAGACA CGTTCCCGTA GCGACGTTGA CCTGGAGGCA 
GCTGCCCGAG CCAACGACTT GCAGGGCACC ACTGGCAAGC TTGGGCCAAA GCCTGGCAAC 
TACACCCCAG AGCCTGGAGG GAGTTCAGAG GGCCCGCTGC CCTTTCTGCT TCCCCAGTCT 
GAGGAGACTG AGCCGCGCGT TCAGGAGGCC GAGAGCGACC CCCCAAACTG GAGGGAGCTC 
GTCCCCCCTG AAATACTCCT CAGGCTGAAG AAAAGCGAAA TCAAGCGGCA AGAGGTCATC 
AATGAGCTCT TCTTTACAGA GCACGCTCAT GTACGGATGC TTCGTGTGTT GCATGATGTC 
TTCTACATCC CCATGCAGAA TGGATGGATC CCCACCTTGG AGCTGGAGAA CATCTTTCCT 
AGCCTGGATG ACCTTATCGA GGCTCATGCT GTATTCCTGG ACACCTTGAA GCGTCTGCGC 
CTTGAGAATA ACTCAGTGAT CCAGGAAATA GGCACCATCT TGCTGAACCG GTTTGACGGC 
ACAGAGGGCA ACTGGTTTCA GAAGATTTCA TCTCGGTTCT GCAGCCGGCA GCCATTTGCC 
TTGGAACAGT TGAAAGCGAA ACAGAAGAGA GACCCTCGAT TTACGCAGTT TATTCAGGAA 
GCTGAGAGCA GCCCTCACTG CCGGCGGTTA CAACTAAAGG ACATTATACC ATTTGAGATG 
CAAAGGCTGA CCAAGTACCC GTTGCTGCTG CAGAACATCG CAAATCTAAC TGAGGAAGAC 
GAGGAGAAGC AGAGAGTGCA GAGGGCAGCA GAGTCTTGCA GGCAGATCCT AAACCATGTT 
AACCAGGAGG TGCGCGAGAT GGAGAATAAG ATGAAACTTA CAGATTACCA AAGACGCCTG 
GATGTTTCCA ACCTGAGGCA AATCAACGAC CCCTTATTTA ATGAATTTAA GAACATTGAT 
ATCAGCAAAA AGAATTTGAT ACATGAAGGA TCTTTGACAT GGAAAGTGAG CAGGGACAAG 
GCTTTTGATG TCCATGTCCT CCTCCTGGAT GATCTCTTGA TGCTGCTGCT GAAACAAGAT 
GAGCGGCTGG TACTGAAGTG CCAGAGTCGG ACTATTATTC CTGCTCCAGA CGGGAAGCTC 
ATGCTGAGCC CAATCATCAA GCTGAATATG GCCATGGTCA GAGAGGTGGC TACAGATAAG 
AATGCCTTTT TTGTGATTTT CAACTGGGAG GATCGAGCAC AGTTCTATGA ACTTGTGGCT 
CCAACACCCG CTGAGAAGAA AAACTGGTTT TTACTGATCA GCAACACTAC AGGCTTATTG 
GAACCCCCAG CTTCTTCCAA ACAGAGACAC AGATCTATCA CTCCTGGCAC AATGCCGCAG 
AGCCCCTCCG ACTTTGCAGA TCAGCTGCTG AGCAGCTCAG AAAATGGAGG CTCCCTGAAA 
GATTCAGTAT CCAAAGATGA AAAGGAAGAG TCGGTGGAGG ATGCATTGGA GGGTCAGGAA 
GAGAAGGACT TCAGCGGCCT TACAGAATAC CTCCTTGCCA GCAACATTGA TCTGAACCAA 
GATGCAGCAT CTGGAGGAGG CAATGTTGCC AGCGAGGCCT TGGAAGAAGT GTCGGTGCTA 
AAACGCCTGC TAGTGCGCAA TATCCGCCTC AGTGTGGATG CAGACACGCT CTCAACCCTG 
GAGACCTGGT CAGCGGGGGA AGAACCTGCA CTGGAGGCTT CTGAACAGGG AAGGGCCTCC 
AATGGGGATC GAGAGGCAGA GGATTCTGAA ATGGAATCTA CACAACAGGG AGAGAATGGC 
AGAAAGCAGA GTTCTGCAGA TGTTTCAGAG TCCCTGTGCG CACCCATTGT ACTGTCCAAA 
GAACAGTCGG AGGAGGTGAA GAGGCACATT CTGTCACTGG AGCAGAGGAT CCAGCAGCTT 
AAGCTGATCG AGGAAGATTA CAACAACCTG CACAGAACAA TCGCCAAATT CACTGTACCG 
AGGAGCAGCT TTACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgef1 ( XM_012967443.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012822897.1 Xenopus tropicalis Rho guanine nucleotide exchange factor 1 (arhgef1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa29991
Clone ID Related Accession (Same CDS sequence)	XM_012967443.2
Accession Version	XM_012967443.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3375bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho guanine nucleotide exchange factor 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012967443.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTCACT CTGTCTACCT CTTACCCTCC TGCATGAGTT ATTGTGGATT CCAAAAGCAC 
GGCTCACAGG TTTCTCCTGG TCTGTGCATC ACTTATGCAT CAGCCGACCT GAGCAGGGGA 
TTCCCATTGT CTCACTCACT GGGTTTTTGC CAGACTGCTC CCAAACCCCT CGTCTGCCAG 
ATAAGAAGCA TGGATCCGGA CGATGGCCAT TTTGGGGGTT TGTACTCCAA GGGGTCCATG 
TCGATCATTG GAGCAGAGGA TGAGGATTTT GAGAATGACA TTGAACCGAA TATGGTTGAT 
GAGAGCAGTT ACTTCCGAAG CATTGAGCTA GTGAAAGAAC ACCCGGCCTA CATTATGGTG 
TTCTTGCAGC ACGTGGTTCT TCAGTTTGAC CCCAGCCCAC TGCTCTGTTT CCTGCATGCG 
GAGATGCTAA AGAACCTTTC AGCCAAAGAG CTTAAGAAAG TCTTCATGGA TTTTCACTAC 
ACATTTCTGA TGAAAGGAGC GGTACTGCAT GTCAGTGTGC CAAACAGCGT ATCCTTTGAA 
CTGGATCGGA CAAGGCCTGA GATGATCCCA GAAGAAACCC AGAGAAAATA TGTCAAAGAC 
ATTCAGAAGC ACCAAACTGA GGCCATTTCC CAACAGCTGG AGGACTTTAG GGCCAAGCGA 
GTACTTGGTA TGACCCCTGG GGAACTGGAG CTGAGCGAGC TTGAATCCTA TCGAACCAAC 
GACCGCAGTA TCCTGGAAGC CAAAGAGAAG CAGTTGGCAG AGCAGCTCCT GGCACGGCTG 
GATGAGATGC ACCTAACTAT TGCTCCAGAC GAGGAAAAAA GCTCTGCCAT CTTTTGTGCC 
ATTGTAGCCT ACATGAAGCA CCTGGGGGTG AAGACCAAGG CGGCGGATAG CAAAAAATCT 
AAAGGAATCT TCTTCAGGAA AAAGGTTTCT ACAAATAAAA AGGATGAGCC TCTGAAAACA 
AAGAAAACAT TCAGCATCTT GGATCCTATG CGCTGGAACC GAAATGATTC TCACTATGTT 
GACAGCAGAC CATCCAAAGT TGAGCCCGAA GCTGAGAAGC CAAACCCGGA CAGAAGGGTC 
AGCCAGGGGC AGAGAACTTT TGATAGGAGT GACAGCAGCA CAGCGAAATC CAAGAACAGC 
GAAGGGCCTG ATTCTGTGGT GTCTGTAAAT ATCATCCCCC CTCCTAATGA GCCCAATGAT 
CCTGACCCAG CAGTGACTGA TGGGGCATTG TCTAATGATG CATCTGAGGT CCTCAAAGGA 
TCCCCCACCA CTGAGCATTG CCCAGCAGAC CAGACTGAGG AAAGTGCAGA AAATGAAAGG 
CTCTCTGGTA AGCTGGGCCG CTCGGAGAGC CTACGAGTTT ACGAGCGCAA GCGTTCCCAG 
CGCGGCTCCT CCAAAGCCAA GCAGACACGT TCCCGTAGCG ACGTTGACCT GGAGGCAGCT 
GCCCGAGCCA ACGACTTGCA GGGCACCACT GGCAAGCTTG GGCCAAAGCC TGGCAACTAC 
ACCCCAGAGC CTGGAGGGAG TTCAGAGGGC CCGCTGCCCT TTCTGCTTCC CCAGTCTGAG 
GAGACTGAGC CGCGCGTTCA GGAGGCCGAG AGCGACCCCC CAAACTGGAG GGAGCTCGTC 
CCCCCTGAAA TACTCCTCAG GCTGAAGAAA AGCGAAATCA AGCGGCAAGA GGTCATCAAT 
GAGCTCTTCT TTACAGAGCA CGCTCATGTA CGGATGCTTC GTGTGTTGCA TGATGTCTTC 
TACATCCCCA TGCAGAATGG ATGGATCCCC ACCTTGGAGC TGGAGAACAT CTTTCCTAGC 
CTGGATGACC TTATCGAGGC TCATGCTGTA TTCCTGGACA CCTTGAAGCG TCTGCGCCTT 
GAGAATAACT CAGTGATCCA GGAAATAGGC ACCATCTTGC TGAACCGGTT TGACGGCACA 
GAGGGCAACT GGTTTCAGAA GATTTCATCT CGGTTCTGCA GCCGGCAGCC ATTTGCCTTG 
GAACAGTTGA AAGCGAAACA GAAGAGAGAC CCTCGATTTA CGCAGTTTAT TCAGGAAGCT 
GAGAGCAGCC CTCACTGCCG GCGGTTACAA CTAAAGGACA TTATACCATT TGAGATGCAA 
AGGCTGACCA AGTACCCGTT GCTGCTGCAG AACATCGCAA ATCTAACTGA GGAAGACGAG 
GAGAAGCAGA GAGTGCAGAG GGCAGCAGAG TCTTGCAGGC AGATCCTAAA CCATGTTAAC 
CAGGAGGTGC GCGAGATGGA GAATAAGATG AAACTTGCAG ATTACCAAAG ACGCCTGGAT 
GTTTCCAACC TGAGGCAAAT CAACGACCCC TTATTTAATG AATTTAAGAA CATTGATATC 
AGCAAAAAGA ATTTGATACA TGAAGGATCT TTGACATGGA AAGTGAGCAG GGACAAGGCT 
TTTGATGTCC ATGTCCTCCT CCTGGATGAT CTCTTGATGC TGCTGCTGAA ACAAGATGAG 
CGGCTGGTAC TGAAGTGCCA GAGTCGGACT ATTATTCCTG CTCCAGACGG GAAGCTCATG 
CTGAGCCCAA TCATCAAGCT GAATATGGCC ATGGTCAGAG AGGTGGCTAC AGATAAGAAT 
GCCTTTTTTG TGATTTTCAA CTGGGAGGAT CGAGCACAGT TCTATGAACT TGTGGCTCCA 
ACACCCGCTG AGAAGAAAAA CTGGTTTTTA CTGATCAGCA ACACTACAGG CTTATTGGAA 
CCCCCAGCTT CTTCCAAACA GAGACACAGA TCTATCACTC CTGGCACAAT GCCGCAGAGC 
CCCTCCGACT TTGCAGATCA GCTGCTGAGC AGCTCAGAAA ATGGAGGCTC CCTGAAAGAT 
TCAGTATCCA AAGATGAAAA GGAAGAGTCG GTGGAGGATG CATTGGAAGG TCAGGAAGAG 
AAGGACTTCA GCGGCCTTAC AGAATACCTC CTTGCCAGCA ACATTGATCT GAACCAAGAT 
GCAGCATCTG GAGGAGGCAA TGTTGCCAGC GAGGCCTTGG AAGAAGTGTC GGTGCTAAAA 
CGCCTGCTAG TGCGCAATAT CCGCCTCAGT GTGGATGCAG ACACGCTCTC AACCCTGGAG 
ACCTGGTCAG CGGGGGAAGA ACCTGCACTG GAGGCTTCTG AACAGGGAAG GGCCTCCAAT 
GGGGATCGAG AGGCAGAGGA TTCTGAAATG GAATCTACAC AACAGGGAGA GAATGGCAGA 
AAGCAGAGTT CTGCAGATGT TGCAGAGTCC CTGTGCGCAC CCATTGTACT GTCCAAAGAA 
CAGTCGGAGG AGGTGAAGAG GCACATTCTG TCACTGGAGC AGAGGATCCA GCAGCTTAAG 
CTGATCGAGG AAGATTACAA CAACCTGCAC AGAACAATCG CCAAATTCAC TGTACCGAGG 
AGCAGCTTTA CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012822897.1
CDS	16..3390
Translation	MTHSVYLLPSCMSYCGFQKHGSQVSPGLCITYASADLSRGFPLSHSLGFCQTAPKPLVCQIRSMDPDDGHFGGLYSKGSMSIIGAEDEDFENDIEPNMVDESSYFRSIELVKEHPAYIMVFLQHVVLQFDPSPLLCFLHAEMLKNLSAKELKKVFMDFHYTFLMKGAVLHVSVPNSVSFELDRTRPEMIPEETQRKYVKDIQKHQTEAISQQLEDFRAKRVLGMTPGELELSELESYRTNDRSILEAKEKQLAEQLLARLDEMHLTIAPDEEKSSAIFCAIVAYMKHLGVKTKAADSKKSKGIFFRKKVSTNKKDEPLKTKKTFSILDPMRWNRNDSHYVDSRPSKVEPEAEKPNPDRRVSQGQRTFDRSDSSTAKSKNSEGPDSVVSVNIIPPPNEPNDPDPAVTDGALSNDASEVLKGSPTTEHCPADQTEESAENERLSGKLGRSESLRVYERKRSQRGSSKAKQTRSRSDVDLEAAARANDLQGTTGKLGPKPGNYTPEPGGSSEGPLPFLLPQSEETEPRVQEAESDPPNWRELVPPEILLRLKKSEIKRQEVINELFFTEHAHVRMLRVLHDVFYIPMQNGWIPTLELENIFPSLDDLIEAHAVFLDTLKRLRLENNSVIQEIGTILLNRFDGTEGNWFQKISSRFCSRQPFALEQLKAKQKRDPRFTQFIQEAESSPHCRRLQLKDIIPFEMQRLTKYPLLLQNIANLTEEDEEKQRVQRAAESCRQILNHVNQEVREMENKMKLADYQRRLDVSNLRQINDPLFNEFKNIDISKKNLIHEGSLTWKVSRDKAFDVHVLLLDDLLMLLLKQDERLVLKCQSRTIIPAPDGKLMLSPIIKLNMAMVREVATDKNAFFVIFNWEDRAQFYELVAPTPAEKKNWFLLISNTTGLLEPPASSKQRHRSITPGTMPQSPSDFADQLLSSSENGGSLKDSVSKDEKEESVEDALEGQEEKDFSGLTEYLLASNIDLNQDAASGGGNVASEALEEVSVLKRLLVRNIRLSVDADTLSTLETWSAGEEPALEASEQGRASNGDREAEDSEMESTQQGENGRKQSSADVAESLCAPIVLSKEQSEEVKRHILSLEQRIQQLKLIEEDYNNLHRTIAKFTVPRSSFT

Target ORF information:

RefSeq Version	XM_012967443.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho guanine nucleotide exchange factor 1 (arhgef1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012967443.2

ORF Insert Sequence:

ATGACTCACT CTGTCTACCT CTTACCCTCC TGCATGAGTT ATTGTGGATT CCAAAAGCAC 
GGCTCACAGG TTTCTCCTGG TCTGTGCATC ACTTATGCAT CAGCCGACCT GAGCAGGGGA 
TTCCCATTGT CTCACTCACT GGGTTTTTGC CAGACTGCTC CCAAACCCCT CGTCTGCCAG 
ATAAGAAGCA TGGATCCGGA CGATGGCCAT TTTGGGGGTT TGTACTCCAA GGGGTCCATG 
TCGATCATTG GAGCAGAGGA TGAGGATTTT GAGAATGACA TTGAACCGAA TATGGTTGAT 
GAGAGCAGTT ACTTCCGAAG CATTGAGCTA GTGAAAGAAC ACCCGGCCTA CATTATGGTG 
TTCTTGCAGC ACGTGGTTCT TCAGTTTGAC CCCAGCCCAC TGCTCTGTTT CCTGCATGCG 
GAGATGCTAA AGAACCTTTC AGCCAAAGAG CTTAAGAAAG TCTTCATGGA TTTTCACTAC 
ACATTTCTGA TGAAAGGAGC GGTACTGCAT GTCAGTGTGC CAAACAGCGT ATCCTTTGAA 
CTGGATCGGA CAAGGCCTGA GATGATCCCA GAAGAAACCC AGAGAAAATA TGTCAAAGAC 
ATTCAGAAGC ACCAAACTGA GGCCATTTCC CAACAGCTGG AGGACTTTAG GGCCAAGCGA 
GTACTTGGTA TGACCCCTGG GGAACTGGAG CTGAGCGAGC TTGAATCCTA TCGAACCAAC 
GACCGCAGTA TCCTGGAAGC CAAAGAGAAG CAGTTGGCAG AGCAGCTCCT GGCACGGCTG 
GATGAGATGC ACCTAACTAT TGCTCCAGAC GAGGAAAAAA GCTCTGCCAT CTTTTGTGCC 
ATTGTAGCCT ACATGAAGCA CCTGGGGGTG AAGACCAAGG CGGCGGATAG CAAAAAATCT 
AAAGGAATCT TCTTCAGGAA AAAGGTTTCT ACAAATAAAA AGGATGAGCC TCTGAAAACA 
AAGAAAACAT TCAGCATCTT GGATCCTATG CGCTGGAACC GAAATGATTC TCACTATGTT 
GACAGCAGAC CATCCAAAGT TGAGCCCGAA GCTGAGAAGC CAAACCCGGA CAGAAGGGTC 
AGCCAGGGGC AGAGAACTTT TGATAGGAGT GACAGCAGCA CAGCGAAATC CAAGAACAGC 
GAAGGGCCTG ATTCTGTGGT GTCTGTAAAT ATCATCCCCC CTCCTAATGA GCCCAATGAT 
CCTGACCCAG CAGTGACTGA TGGGGCATTG TCTAATGATG CATCTGAGGT CCTCAAAGGA 
TCCCCCACCA CTGAGCATTG CCCAGCAGAC CAGACTGAGG AAAGTGCAGA AAATGAAAGG 
CTCTCTGGTA AGCTGGGCCG CTCGGAGAGC CTACGAGTTT ACGAGCGCAA GCGTTCCCAG 
CGCGGCTCCT CCAAAGCCAA GCAGACACGT TCCCGTAGCG ACGTTGACCT GGAGGCAGCT 
GCCCGAGCCA ACGACTTGCA GGGCACCACT GGCAAGCTTG GGCCAAAGCC TGGCAACTAC 
ACCCCAGAGC CTGGAGGGAG TTCAGAGGGC CCGCTGCCCT TTCTGCTTCC CCAGTCTGAG 
GAGACTGAGC CGCGCGTTCA GGAGGCCGAG AGCGACCCCC CAAACTGGAG GGAGCTCGTC 
CCCCCTGAAA TACTCCTCAG GCTGAAGAAA AGCGAAATCA AGCGGCAAGA GGTCATCAAT 
GAGCTCTTCT TTACAGAGCA CGCTCATGTA CGGATGCTTC GTGTGTTGCA TGATGTCTTC 
TACATCCCCA TGCAGAATGG ATGGATCCCC ACCTTGGAGC TGGAGAACAT CTTTCCTAGC 
CTGGATGACC TTATCGAGGC TCATGCTGTA TTCCTGGACA CCTTGAAGCG TCTGCGCCTT 
GAGAATAACT CAGTGATCCA GGAAATAGGC ACCATCTTGC TGAACCGGTT TGACGGCACA 
GAGGGCAACT GGTTTCAGAA GATTTCATCT CGGTTCTGCA GCCGGCAGCC ATTTGCCTTG 
GAACAGTTGA AAGCGAAACA GAAGAGAGAC CCTCGATTTA CGCAGTTTAT TCAGGAAGCT 
GAGAGCAGCC CTCACTGCCG GCGGTTACAA CTAAAGGACA TTATACCATT TGAGATGCAA 
AGGCTGACCA AGTACCCGTT GCTGCTGCAG AACATCGCAA ATCTAACTGA GGAAGACGAG 
GAGAAGCAGA GAGTGCAGAG GGCAGCAGAG TCTTGCAGGC AGATCCTAAA CCATGTTAAC 
CAGGAGGTGC GCGAGATGGA GAATAAGATG AAACTTGCAG ATTACCAAAG ACGCCTGGAT 
GTTTCCAACC TGAGGCAAAT CAACGACCCC TTATTTAATG AATTTAAGAA CATTGATATC 
AGCAAAAAGA ATTTGATACA TGAAGGATCT TTGACATGGA AAGTGAGCAG GGACAAGGCT 
TTTGATGTCC ATGTCCTCCT CCTGGATGAT CTCTTGATGC TGCTGCTGAA ACAAGATGAG 
CGGCTGGTAC TGAAGTGCCA GAGTCGGACT ATTATTCCTG CTCCAGACGG GAAGCTCATG 
CTGAGCCCAA TCATCAAGCT GAATATGGCC ATGGTCAGAG AGGTGGCTAC AGATAAGAAT 
GCCTTTTTTG TGATTTTCAA CTGGGAGGAT CGAGCACAGT TCTATGAACT TGTGGCTCCA 
ACACCCGCTG AGAAGAAAAA CTGGTTTTTA CTGATCAGCA ACACTACAGG CTTATTGGAA 
CCCCCAGCTT CTTCCAAACA GAGACACAGA TCTATCACTC CTGGCACAAT GCCGCAGAGC 
CCCTCCGACT TTGCAGATCA GCTGCTGAGC AGCTCAGAAA ATGGAGGCTC CCTGAAAGAT 
TCAGTATCCA AAGATGAAAA GGAAGAGTCG GTGGAGGATG CATTGGAAGG TCAGGAAGAG 
AAGGACTTCA GCGGCCTTAC AGAATACCTC CTTGCCAGCA ACATTGATCT GAACCAAGAT 
GCAGCATCTG GAGGAGGCAA TGTTGCCAGC GAGGCCTTGG AAGAAGTGTC GGTGCTAAAA 
CGCCTGCTAG TGCGCAATAT CCGCCTCAGT GTGGATGCAG ACACGCTCTC AACCCTGGAG 
ACCTGGTCAG CGGGGGAAGA ACCTGCACTG GAGGCTTCTG AACAGGGAAG GGCCTCCAAT 
GGGGATCGAG AGGCAGAGGA TTCTGAAATG GAATCTACAC AACAGGGAGA GAATGGCAGA 
AAGCAGAGTT CTGCAGATGT TGCAGAGTCC CTGTGCGCAC CCATTGTACT GTCCAAAGAA 
CAGTCGGAGG AGGTGAAGAG GCACATTCTG TCACTGGAGC AGAGGATCCA GCAGCTTAAG 
CTGATCGAGG AAGATTACAA CAACCTGCAC AGAACAATCG CCAAATTCAC TGTACCGAGG 
AGCAGCTTTA CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgef1 ( XM_004916244.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004916301.2 Xenopus tropicalis Rho guanine nucleotide exchange factor 1 (arhgef1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OXa29994
Clone ID Related Accession (Same CDS sequence)	XM_004916244.3
Accession Version	XM_004916244.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3192bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho guanine nucleotide exchange factor 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004916244.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTCACT CTGTCTACCT CTTACCCTCC TGCATGAGTT ATTGTGGATT CCAAAAGCAC 
GGCTCACAGG TTTCTCCTGG TCTGTGCATC ACTTATGCAT CAGCCGACCT GAGCAGGGGA 
TTCCCATTGT CTCACTCACT GGGTTTTTGC CAGACTGCTC CCAAACCCCT CGTCTGCCAG 
ATAAGAAGCA TGGATCCGGA CGATGGCCAT TTTGGGGGTT TGTACTCCAA GGGGTCCATG 
TCGATCATTG GAGCAGAGGA TGAGGATTTT GAGAATGACA TTGAACCGAA TATGGTTGAT 
GAGAGCAGTT ACTTCCGAAG CATTGAGCTA GTGAAAGAAC ACCCGGCCTA CATTATGGTG 
TTCTTGCAGC ACGTGGTTCT TCAGTTTGAC CCCAGCCCAC TGCTCTGTTT CCTGCATGCG 
GAGATGCTAA AGAACCTTTC AGCCAAAGAG CTTAAGAAAG TCTTCATGGA TTTTCACTAC 
ACATTTCTGA TGAAAGGAGC GGTACTGCAT GTCAGTGTGC CAAACAGCGT ATCCTTTGAA 
CTGGATCGGA CAAGGCCTGA GATGATCCCA GAAGAAACCC AGAGAAAATA TGTCAAAGAC 
ATTCAGAAGC ACCAAACTGA GGCCATTTCC CAACAGCTGG AGGACTTTAG GGCCAAGCGA 
GTACTTGGTA TGACCCCTGG GGAACTGGAG CTGAGCGAGC TTGAATCCTA TCGAACCAAC 
GACCGCAGTA TCCTGGAAGC CAAAGAGAAG CAGTTGGCAG AGCAGCTCCT GGCACGGCTG 
GATGAGATGC ACCTAACTAT TGCTCCAGAC GAGGAAAAAA GCTCTGCCAT CTTTTGTGCC 
ATTGTAGCCT ACATGAAGCA CCTGGGGGTG AAGACCAAGG CGGCGGATAG CAAAAAATCT 
AAAGGAATCT TCTTCAGGAA AAAGGTTTCT ACAAATAAAA AGGATGAGCC TCTGAAAACA 
AAGAAAACAT TCAGCATCTT GGATCCTATG CGCTGGAACC GAAATGATTC TCACTATGTT 
GACAGCAGAC CATCCAAAGT TGAGCCCGAA GCTGAGAAGC CAAACCCGGA CAGAAGGGTC 
AGCCAGGGGC AGAGAACTTT TGATAGGAGT GACAGCAGCA CAGCGAAATC CAAGAACAGC 
GAAGGGCCTG ATTCTGTGGT GTCTGTAAAT ATCATCCCCC CTCCTAATGA GCCCAATGAT 
CCTGACCCAG CAGTGACTGA TGGGGCATTG TCTAATGATG CATCTGAGGT CCTCAAAGGA 
TCCCCCACCA CTGAGCATTG CCCAGCAGAC CAGACTGAGG AAAGTGCAGA AAATGAAAGC 
CCAGAGCCTG GAGGGAGTTC AGAGGGCCCG CTGCCCTTTC TGCTTCCCCA GTCTGAGGAG 
ACTGAGCCGC GCGTTCAGGA GGCCGAGAGC GACCCCCCAA ACTGGAGGGA GCTCGTCCCC 
CCTGAAATAC TCCTCAGGCT GAAGAAAAGC GAAATCAAGC GGCAAGAGGT CATCAATGAG 
CTCTTCTTTA CAGAGCACGC TCATGTACGG ATGCTTCGTG TGTTGCATGA TGTCTTCTAC 
ATCCCCATGC AGAATGGATG GATCCCCACC TTGGAGCTGG AGAACATCTT TCCTAGCCTG 
GATGACCTTA TCGAGGCTCA TGCTGTATTC CTGGACACCT TGAAGCGTCT GCGCCTTGAG 
AATAACTCAG TGATCCAGGA AATAGGCACC ATCTTGCTGA ACCGGTTTGA CGGCACAGAG 
GGCAACTGGT TTCAGAAGAT TTCATCTCGG TTCTGCAGCC GGCAGCCATT TGCCTTGGAA 
CAGTTGAAAG CGAAACAGAA GAGAGACCCT CGATTTACGC AGTTTATTCA GGAAGCTGAG 
AGCAGCCCTC ACTGCCGGCG GTTACAACTA AAGGACATTA TACCATTTGA GATGCAAAGG 
CTGACCAAGT ACCCGTTGCT GCTGCAGAAC ATCGCAAATC TAACTGAGGA AGACGAGGAG 
AAGCAGAGAG TGCAGAGGGC AGCAGAGTCT TGCAGGCAGA TCCTAAACCA TGTTAACCAG 
GAGGTGCGCG AGATGGAGAA TAAGATGAAA CTTGCAGATT ACCAAAGACG CCTGGATGTT 
TCCAACCTGA GGCAAATCAA CGACCCCTTA TTTAATGAAT TTAAGAACAT TGATATCAGC 
AAAAAGAATT TGATACATGA AGGATCTTTG ACATGGAAAG TGAGCAGGGA CAAGGCTTTT 
GATGTCCATG TCCTCCTCCT GGATGATCTC TTGATGCTGC TGCTGAAACA AGATGAGCGG 
CTGGTACTGA AGTGCCAGAG TCGGACTATT ATTCCTGCTC CAGACGGGAA GCTCATGCTG 
AGCCCAATCA TCAAGCTGAA TATGGCCATG GTCAGAGAGG TGGCTACAGA TAAGAATGCC 
TTTTTTGTGA TTTTCAACTG GGAGGATCGA GCACAGTTCT ATGAACTTGT GGCTCCAACA 
CCCGCTGAGA AGAAAAACTG GTTTTTACTG ATCAGCAACA CTACAGGCTT ATTGGAACCC 
CCAGCTTCTT CCAAACAGAG ACACAGATCT ATCACTCCTG GCACAATGCC GCAGAGCCCC 
TCCGACTTTG CAGATCAGCT GCTGAGCAGC TCAGAAAATG GAGGCTCCCT GAAAGATTCA 
GTATCCAAAG ATGAAAAGGA AGAGTCGGTG GAGGATGCAT TGGAAGGTCA GGAAGAGAAG 
GACTTCAGCG GCCTTACAGA ATACCTCCTT GCCAGCAACA TTGATCTGAA CCAAGATGCA 
GCATCTGGAG GAGGCAATGT TGCCAGCGAG GCCTTGGAAG AAGTGTCGGT GCTAAAACGC 
CTGCTAGTGC GCAATATCCG CCTCAGTGTG GATGCAGACA CGCTCTCAAC CCTGGAGACC 
TGGTCAGCGG GGGAAGAACC TGCACTGGAG GCTTCTGAAC AGGGAAGGGC CTCCAATGGG 
GATCGAGAGG CAGAGGATTC TGAAATGGAA TCTACACAAC AGGGAGAGAA TGGCAGAAAG 
CAGAGTTCTG CAGATGTTGC AGAGTCCCTG TGCGCACCCA TTGTACTGTC CAAAGAACAG 
TCGGAGGAGG TGAAGAGGCA CATTCTGTCA CTGGAGCAGA GGATCCAGCA GCTTAAGCTG 
ATCGAGGAAG ATTACAACAA CCTGCACAGA ACAATCGCCA AATTCACTGT ACCGAGGAGC 
AGCTTTACAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004916301.2
CDS	16..3207
Translation	MTHSVYLLPSCMSYCGFQKHGSQVSPGLCITYASADLSRGFPLSHSLGFCQTAPKPLVCQIRSMDPDDGHFGGLYSKGSMSIIGAEDEDFENDIEPNMVDESSYFRSIELVKEHPAYIMVFLQHVVLQFDPSPLLCFLHAEMLKNLSAKELKKVFMDFHYTFLMKGAVLHVSVPNSVSFELDRTRPEMIPEETQRKYVKDIQKHQTEAISQQLEDFRAKRVLGMTPGELELSELESYRTNDRSILEAKEKQLAEQLLARLDEMHLTIAPDEEKSSAIFCAIVAYMKHLGVKTKAADSKKSKGIFFRKKVSTNKKDEPLKTKKTFSILDPMRWNRNDSHYVDSRPSKVEPEAEKPNPDRRVSQGQRTFDRSDSSTAKSKNSEGPDSVVSVNIIPPPNEPNDPDPAVTDGALSNDASEVLKGSPTTEHCPADQTEESAENESPEPGGSSEGPLPFLLPQSEETEPRVQEAESDPPNWRELVPPEILLRLKKSEIKRQEVINELFFTEHAHVRMLRVLHDVFYIPMQNGWIPTLELENIFPSLDDLIEAHAVFLDTLKRLRLENNSVIQEIGTILLNRFDGTEGNWFQKISSRFCSRQPFALEQLKAKQKRDPRFTQFIQEAESSPHCRRLQLKDIIPFEMQRLTKYPLLLQNIANLTEEDEEKQRVQRAAESCRQILNHVNQEVREMENKMKLADYQRRLDVSNLRQINDPLFNEFKNIDISKKNLIHEGSLTWKVSRDKAFDVHVLLLDDLLMLLLKQDERLVLKCQSRTIIPAPDGKLMLSPIIKLNMAMVREVATDKNAFFVIFNWEDRAQFYELVAPTPAEKKNWFLLISNTTGLLEPPASSKQRHRSITPGTMPQSPSDFADQLLSSSENGGSLKDSVSKDEKEESVEDALEGQEEKDFSGLTEYLLASNIDLNQDAASGGGNVASEALEEVSVLKRLLVRNIRLSVDADTLSTLETWSAGEEPALEASEQGRASNGDREAEDSEMESTQQGENGRKQSSADVAESLCAPIVLSKEQSEEVKRHILSLEQRIQQLKLIEEDYNNLHRTIAKFTVPRSSFT

Target ORF information:

RefSeq Version	XM_004916244.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho guanine nucleotide exchange factor 1 (arhgef1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004916244.3

ORF Insert Sequence:

ATGACTCACT CTGTCTACCT CTTACCCTCC TGCATGAGTT ATTGTGGATT CCAAAAGCAC 
GGCTCACAGG TTTCTCCTGG TCTGTGCATC ACTTATGCAT CAGCCGACCT GAGCAGGGGA 
TTCCCATTGT CTCACTCACT GGGTTTTTGC CAGACTGCTC CCAAACCCCT CGTCTGCCAG 
ATAAGAAGCA TGGATCCGGA CGATGGCCAT TTTGGGGGTT TGTACTCCAA GGGGTCCATG 
TCGATCATTG GAGCAGAGGA TGAGGATTTT GAGAATGACA TTGAACCGAA TATGGTTGAT 
GAGAGCAGTT ACTTCCGAAG CATTGAGCTA GTGAAAGAAC ACCCGGCCTA CATTATGGTG 
TTCTTGCAGC ACGTGGTTCT TCAGTTTGAC CCCAGCCCAC TGCTCTGTTT CCTGCATGCG 
GAGATGCTAA AGAACCTTTC AGCCAAAGAG CTTAAGAAAG TCTTCATGGA TTTTCACTAC 
ACATTTCTGA TGAAAGGAGC GGTACTGCAT GTCAGTGTGC CAAACAGCGT ATCCTTTGAA 
CTGGATCGGA CAAGGCCTGA GATGATCCCA GAAGAAACCC AGAGAAAATA TGTCAAAGAC 
ATTCAGAAGC ACCAAACTGA GGCCATTTCC CAACAGCTGG AGGACTTTAG GGCCAAGCGA 
GTACTTGGTA TGACCCCTGG GGAACTGGAG CTGAGCGAGC TTGAATCCTA TCGAACCAAC 
GACCGCAGTA TCCTGGAAGC CAAAGAGAAG CAGTTGGCAG AGCAGCTCCT GGCACGGCTG 
GATGAGATGC ACCTAACTAT TGCTCCAGAC GAGGAAAAAA GCTCTGCCAT CTTTTGTGCC 
ATTGTAGCCT ACATGAAGCA CCTGGGGGTG AAGACCAAGG CGGCGGATAG CAAAAAATCT 
AAAGGAATCT TCTTCAGGAA AAAGGTTTCT ACAAATAAAA AGGATGAGCC TCTGAAAACA 
AAGAAAACAT TCAGCATCTT GGATCCTATG CGCTGGAACC GAAATGATTC TCACTATGTT 
GACAGCAGAC CATCCAAAGT TGAGCCCGAA GCTGAGAAGC CAAACCCGGA CAGAAGGGTC 
AGCCAGGGGC AGAGAACTTT TGATAGGAGT GACAGCAGCA CAGCGAAATC CAAGAACAGC 
GAAGGGCCTG ATTCTGTGGT GTCTGTAAAT ATCATCCCCC CTCCTAATGA GCCCAATGAT 
CCTGACCCAG CAGTGACTGA TGGGGCATTG TCTAATGATG CATCTGAGGT CCTCAAAGGA 
TCCCCCACCA CTGAGCATTG CCCAGCAGAC CAGACTGAGG AAAGTGCAGA AAATGAAAGC 
CCAGAGCCTG GAGGGAGTTC AGAGGGCCCG CTGCCCTTTC TGCTTCCCCA GTCTGAGGAG 
ACTGAGCCGC GCGTTCAGGA GGCCGAGAGC GACCCCCCAA ACTGGAGGGA GCTCGTCCCC 
CCTGAAATAC TCCTCAGGCT GAAGAAAAGC GAAATCAAGC GGCAAGAGGT CATCAATGAG 
CTCTTCTTTA CAGAGCACGC TCATGTACGG ATGCTTCGTG TGTTGCATGA TGTCTTCTAC 
ATCCCCATGC AGAATGGATG GATCCCCACC TTGGAGCTGG AGAACATCTT TCCTAGCCTG 
GATGACCTTA TCGAGGCTCA TGCTGTATTC CTGGACACCT TGAAGCGTCT GCGCCTTGAG 
AATAACTCAG TGATCCAGGA AATAGGCACC ATCTTGCTGA ACCGGTTTGA CGGCACAGAG 
GGCAACTGGT TTCAGAAGAT TTCATCTCGG TTCTGCAGCC GGCAGCCATT TGCCTTGGAA 
CAGTTGAAAG CGAAACAGAA GAGAGACCCT CGATTTACGC AGTTTATTCA GGAAGCTGAG 
AGCAGCCCTC ACTGCCGGCG GTTACAACTA AAGGACATTA TACCATTTGA GATGCAAAGG 
CTGACCAAGT ACCCGTTGCT GCTGCAGAAC ATCGCAAATC TAACTGAGGA AGACGAGGAG 
AAGCAGAGAG TGCAGAGGGC AGCAGAGTCT TGCAGGCAGA TCCTAAACCA TGTTAACCAG 
GAGGTGCGCG AGATGGAGAA TAAGATGAAA CTTGCAGATT ACCAAAGACG CCTGGATGTT 
TCCAACCTGA GGCAAATCAA CGACCCCTTA TTTAATGAAT TTAAGAACAT TGATATCAGC 
AAAAAGAATT TGATACATGA AGGATCTTTG ACATGGAAAG TGAGCAGGGA CAAGGCTTTT 
GATGTCCATG TCCTCCTCCT GGATGATCTC TTGATGCTGC TGCTGAAACA AGATGAGCGG 
CTGGTACTGA AGTGCCAGAG TCGGACTATT ATTCCTGCTC CAGACGGGAA GCTCATGCTG 
AGCCCAATCA TCAAGCTGAA TATGGCCATG GTCAGAGAGG TGGCTACAGA TAAGAATGCC 
TTTTTTGTGA TTTTCAACTG GGAGGATCGA GCACAGTTCT ATGAACTTGT GGCTCCAACA 
CCCGCTGAGA AGAAAAACTG GTTTTTACTG ATCAGCAACA CTACAGGCTT ATTGGAACCC 
CCAGCTTCTT CCAAACAGAG ACACAGATCT ATCACTCCTG GCACAATGCC GCAGAGCCCC 
TCCGACTTTG CAGATCAGCT GCTGAGCAGC TCAGAAAATG GAGGCTCCCT GAAAGATTCA 
GTATCCAAAG ATGAAAAGGA AGAGTCGGTG GAGGATGCAT TGGAAGGTCA GGAAGAGAAG 
GACTTCAGCG GCCTTACAGA ATACCTCCTT GCCAGCAACA TTGATCTGAA CCAAGATGCA 
GCATCTGGAG GAGGCAATGT TGCCAGCGAG GCCTTGGAAG AAGTGTCGGT GCTAAAACGC 
CTGCTAGTGC GCAATATCCG CCTCAGTGTG GATGCAGACA CGCTCTCAAC CCTGGAGACC 
TGGTCAGCGG GGGAAGAACC TGCACTGGAG GCTTCTGAAC AGGGAAGGGC CTCCAATGGG 
GATCGAGAGG CAGAGGATTC TGAAATGGAA TCTACACAAC AGGGAGAGAA TGGCAGAAAG 
CAGAGTTCTG CAGATGTTGC AGAGTCCCTG TGCGCACCCA TTGTACTGTC CAAAGAACAG 
TCGGAGGAGG TGAAGAGGCA CATTCTGTCA CTGGAGCAGA GGATCCAGCA GCTTAAGCTG 
ATCGAGGAAG ATTACAACAA CCTGCACAGA ACAATCGCCA AATTCACTGT ACCGAGGAGC 
AGCTTTACAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgef1 ( XM_004916245.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004916302.2 Xenopus tropicalis Rho guanine nucleotide exchange factor 1 (arhgef1), transcript variant X7, mRNA.

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CloneID	OXa29995
Clone ID Related Accession (Same CDS sequence)	XM_004916245.3
Accession Version	XM_004916245.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3156bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho guanine nucleotide exchange factor 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004916245.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTCACT CTGTCTACCT CTTACCCTCC TGCATGAGTT ATTGTGGATT CCAAAAGCAC 
GGCTCACAGG TTTCTCCTGG TCTGTGCATC ACTTATGCAT CAGCCGACCT GAGCAGGGGA 
TTCCCATTGT CTCACTCACT GGGTTTTTGC CAGACTGCTC CCAAACCCCT CGTCTGCCAG 
ATAAGAAGCA TGGATCCGGA CGATGGCCAT TTTGGGGGTT TGTACTCCAA GGGGTCCATG 
TCGATCATTG GAGCAGAGGA TGAGGATTTT GAGAATGACA TTGAACCGAA TATGGTTGAT 
GAGAGCAGTT ACTTCCGAAG CATTGAGCTA GTGAAAGAAC ACCCGGCCTA CATTATGGTG 
TTCTTGCAGC ACGTGGTTCT TCAGTTTGAC CCCAGCCCAC TGCTCTGTTT CCTGCATGCG 
GAGATGCTAA AGAACCTTTC AGCCAAAGAG CTTAAGAAAG TCTTCATGGA TTTTCACTAC 
ACATTTCTGA TGAAAGGAGC GGTACTGCAT GTCAGTGTGC CAAACAGCGT ATCCTTTGAA 
CTGGATCGGA CAAGGCCTGA GATGATCCCA GAAGAAACCC AGAGAAAATA TGTCAAAGAC 
ATTCAGAAGC ACCAAACTGA GGCCATTTCC CAACAGCTGG AGGACTTTAG GGCCAAGCGA 
GTACTTGGTA TGACCCCTGG GGAACTGGAG CTGAGCGAGC TTGAATCCTA TCGAACCAAC 
GACCGCAGTA TCCTGGAAGC CAAAGAGAAG CAGTTGGCAG AGCAGCTCCT GGCACGGCTG 
GATGAGATGC ACCTAACTAT TGCTCCAGAC GAGGAAAAAA GCTCTGCCAT CTTTTGTGCC 
ATTGTAGCCT ACATGAAGCA CCTGGGGGTG AAGACCAAGG CGGCGGATAG CAAAAAATCT 
AAAGGAATCT TCTTCAGGAA AAAGGTTTCT ACAAATAAAA AGGATGAGCC TCTGAAAACA 
AAGAAAACAT TCAGCATCTT GGATCCTATG CGCTGGAACC GAAATGATTC TCACTCTGAG 
AAGCCAAACC CGGACAGAAG GGTCAGCCAG GGGCAGAGAA CTTTTGATAG GAGTGACAGC 
AGCACAGCGA AATCCAAGAA CAGCGAAGGG CCTGATTCTG TGGTGTCTGT AAATATCATC 
CCCCCTCCTA ATGAGCCCAA TGATCCTGAC CCAGCAGTGA CTGATGGGGC ATTGTCTAAT 
GATGCATCTG AGGTCCTCAA AGGATCCCCC ACCACTGAGC ATTGCCCAGC AGACCAGACT 
GAGGAAAGTG CAGAAAATGA AAGCCCAGAG CCTGGAGGGA GTTCAGAGGG CCCGCTGCCC 
TTTCTGCTTC CCCAGTCTGA GGAGACTGAG CCGCGCGTTC AGGAGGCCGA GAGCGACCCC 
CCAAACTGGA GGGAGCTCGT CCCCCCTGAA ATACTCCTCA GGCTGAAGAA AAGCGAAATC 
AAGCGGCAAG AGGTCATCAA TGAGCTCTTC TTTACAGAGC ACGCTCATGT ACGGATGCTT 
CGTGTGTTGC ATGATGTCTT CTACATCCCC ATGCAGAATG GATGGATCCC CACCTTGGAG 
CTGGAGAACA TCTTTCCTAG CCTGGATGAC CTTATCGAGG CTCATGCTGT ATTCCTGGAC 
ACCTTGAAGC GTCTGCGCCT TGAGAATAAC TCAGTGATCC AGGAAATAGG CACCATCTTG 
CTGAACCGGT TTGACGGCAC AGAGGGCAAC TGGTTTCAGA AGATTTCATC TCGGTTCTGC 
AGCCGGCAGC CATTTGCCTT GGAACAGTTG AAAGCGAAAC AGAAGAGAGA CCCTCGATTT 
ACGCAGTTTA TTCAGGAAGC TGAGAGCAGC CCTCACTGCC GGCGGTTACA ACTAAAGGAC 
ATTATACCAT TTGAGATGCA AAGGCTGACC AAGTACCCGT TGCTGCTGCA GAACATCGCA 
AATCTAACTG AGGAAGACGA GGAGAAGCAG AGAGTGCAGA GGGCAGCAGA GTCTTGCAGG 
CAGATCCTAA ACCATGTTAA CCAGGAGGTG CGCGAGATGG AGAATAAGAT GAAACTTGCA 
GATTACCAAA GACGCCTGGA TGTTTCCAAC CTGAGGCAAA TCAACGACCC CTTATTTAAT 
GAATTTAAGA ACATTGATAT CAGCAAAAAG AATTTGATAC ATGAAGGATC TTTGACATGG 
AAAGTGAGCA GGGACAAGGC TTTTGATGTC CATGTCCTCC TCCTGGATGA TCTCTTGATG 
CTGCTGCTGA AACAAGATGA GCGGCTGGTA CTGAAGTGCC AGAGTCGGAC TATTATTCCT 
GCTCCAGACG GGAAGCTCAT GCTGAGCCCA ATCATCAAGC TGAATATGGC CATGGTCAGA 
GAGGTGGCTA CAGATAAGAA TGCCTTTTTT GTGATTTTCA ACTGGGAGGA TCGAGCACAG 
TTCTATGAAC TTGTGGCTCC AACACCCGCT GAGAAGAAAA ACTGGTTTTT ACTGATCAGC 
AACACTACAG GCTTATTGGA ACCCCCAGCT TCTTCCAAAC AGAGACACAG ATCTATCACT 
CCTGGCACAA TGCCGCAGAG CCCCTCCGAC TTTGCAGATC AGCTGCTGAG CAGCTCAGAA 
AATGGAGGCT CCCTGAAAGA TTCAGTATCC AAAGATGAAA AGGAAGAGTC GGTGGAGGAT 
GCATTGGAAG GTCAGGAAGA GAAGGACTTC AGCGGCCTTA CAGAATACCT CCTTGCCAGC 
AACATTGATC TGAACCAAGA TGCAGCATCT GGAGGAGGCA ATGTTGCCAG CGAGGCCTTG 
GAAGAAGTGT CGGTGCTAAA ACGCCTGCTA GTGCGCAATA TCCGCCTCAG TGTGGATGCA 
GACACGCTCT CAACCCTGGA GACCTGGTCA GCGGGGGAAG AACCTGCACT GGAGGCTTCT 
GAACAGGGAA GGGCCTCCAA TGGGGATCGA GAGGCAGAGG ATTCTGAAAT GGAATCTACA 
CAACAGGGAG AGAATGGCAG AAAGCAGAGT TCTGCAGATG TTGCAGAGTC CCTGTGCGCA 
CCCATTGTAC TGTCCAAAGA ACAGTCGGAG GAGGTGAAGA GGCACATTCT GTCACTGGAG 
CAGAGGATCC AGCAGCTTAA GCTGATCGAG GAAGATTACA ACAACCTGCA CAGAACAATC 
GCCAAATTCA CTGTACCGAG GAGCAGCTTT ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004916302.2
CDS	16..3171
Translation	MTHSVYLLPSCMSYCGFQKHGSQVSPGLCITYASADLSRGFPLSHSLGFCQTAPKPLVCQIRSMDPDDGHFGGLYSKGSMSIIGAEDEDFENDIEPNMVDESSYFRSIELVKEHPAYIMVFLQHVVLQFDPSPLLCFLHAEMLKNLSAKELKKVFMDFHYTFLMKGAVLHVSVPNSVSFELDRTRPEMIPEETQRKYVKDIQKHQTEAISQQLEDFRAKRVLGMTPGELELSELESYRTNDRSILEAKEKQLAEQLLARLDEMHLTIAPDEEKSSAIFCAIVAYMKHLGVKTKAADSKKSKGIFFRKKVSTNKKDEPLKTKKTFSILDPMRWNRNDSHSEKPNPDRRVSQGQRTFDRSDSSTAKSKNSEGPDSVVSVNIIPPPNEPNDPDPAVTDGALSNDASEVLKGSPTTEHCPADQTEESAENESPEPGGSSEGPLPFLLPQSEETEPRVQEAESDPPNWRELVPPEILLRLKKSEIKRQEVINELFFTEHAHVRMLRVLHDVFYIPMQNGWIPTLELENIFPSLDDLIEAHAVFLDTLKRLRLENNSVIQEIGTILLNRFDGTEGNWFQKISSRFCSRQPFALEQLKAKQKRDPRFTQFIQEAESSPHCRRLQLKDIIPFEMQRLTKYPLLLQNIANLTEEDEEKQRVQRAAESCRQILNHVNQEVREMENKMKLADYQRRLDVSNLRQINDPLFNEFKNIDISKKNLIHEGSLTWKVSRDKAFDVHVLLLDDLLMLLLKQDERLVLKCQSRTIIPAPDGKLMLSPIIKLNMAMVREVATDKNAFFVIFNWEDRAQFYELVAPTPAEKKNWFLLISNTTGLLEPPASSKQRHRSITPGTMPQSPSDFADQLLSSSENGGSLKDSVSKDEKEESVEDALEGQEEKDFSGLTEYLLASNIDLNQDAASGGGNVASEALEEVSVLKRLLVRNIRLSVDADTLSTLETWSAGEEPALEASEQGRASNGDREAEDSEMESTQQGENGRKQSSADVAESLCAPIVLSKEQSEEVKRHILSLEQRIQQLKLIEEDYNNLHRTIAKFTVPRSSFT

Target ORF information:

RefSeq Version	XM_004916245.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho guanine nucleotide exchange factor 1 (arhgef1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004916245.3

ORF Insert Sequence:

ATGACTCACT CTGTCTACCT CTTACCCTCC TGCATGAGTT ATTGTGGATT CCAAAAGCAC 
GGCTCACAGG TTTCTCCTGG TCTGTGCATC ACTTATGCAT CAGCCGACCT GAGCAGGGGA 
TTCCCATTGT CTCACTCACT GGGTTTTTGC CAGACTGCTC CCAAACCCCT CGTCTGCCAG 
ATAAGAAGCA TGGATCCGGA CGATGGCCAT TTTGGGGGTT TGTACTCCAA GGGGTCCATG 
TCGATCATTG GAGCAGAGGA TGAGGATTTT GAGAATGACA TTGAACCGAA TATGGTTGAT 
GAGAGCAGTT ACTTCCGAAG CATTGAGCTA GTGAAAGAAC ACCCGGCCTA CATTATGGTG 
TTCTTGCAGC ACGTGGTTCT TCAGTTTGAC CCCAGCCCAC TGCTCTGTTT CCTGCATGCG 
GAGATGCTAA AGAACCTTTC AGCCAAAGAG CTTAAGAAAG TCTTCATGGA TTTTCACTAC 
ACATTTCTGA TGAAAGGAGC GGTACTGCAT GTCAGTGTGC CAAACAGCGT ATCCTTTGAA 
CTGGATCGGA CAAGGCCTGA GATGATCCCA GAAGAAACCC AGAGAAAATA TGTCAAAGAC 
ATTCAGAAGC ACCAAACTGA GGCCATTTCC CAACAGCTGG AGGACTTTAG GGCCAAGCGA 
GTACTTGGTA TGACCCCTGG GGAACTGGAG CTGAGCGAGC TTGAATCCTA TCGAACCAAC 
GACCGCAGTA TCCTGGAAGC CAAAGAGAAG CAGTTGGCAG AGCAGCTCCT GGCACGGCTG 
GATGAGATGC ACCTAACTAT TGCTCCAGAC GAGGAAAAAA GCTCTGCCAT CTTTTGTGCC 
ATTGTAGCCT ACATGAAGCA CCTGGGGGTG AAGACCAAGG CGGCGGATAG CAAAAAATCT 
AAAGGAATCT TCTTCAGGAA AAAGGTTTCT ACAAATAAAA AGGATGAGCC TCTGAAAACA 
AAGAAAACAT TCAGCATCTT GGATCCTATG CGCTGGAACC GAAATGATTC TCACTCTGAG 
AAGCCAAACC CGGACAGAAG GGTCAGCCAG GGGCAGAGAA CTTTTGATAG GAGTGACAGC 
AGCACAGCGA AATCCAAGAA CAGCGAAGGG CCTGATTCTG TGGTGTCTGT AAATATCATC 
CCCCCTCCTA ATGAGCCCAA TGATCCTGAC CCAGCAGTGA CTGATGGGGC ATTGTCTAAT 
GATGCATCTG AGGTCCTCAA AGGATCCCCC ACCACTGAGC ATTGCCCAGC AGACCAGACT 
GAGGAAAGTG CAGAAAATGA AAGCCCAGAG CCTGGAGGGA GTTCAGAGGG CCCGCTGCCC 
TTTCTGCTTC CCCAGTCTGA GGAGACTGAG CCGCGCGTTC AGGAGGCCGA GAGCGACCCC 
CCAAACTGGA GGGAGCTCGT CCCCCCTGAA ATACTCCTCA GGCTGAAGAA AAGCGAAATC 
AAGCGGCAAG AGGTCATCAA TGAGCTCTTC TTTACAGAGC ACGCTCATGT ACGGATGCTT 
CGTGTGTTGC ATGATGTCTT CTACATCCCC ATGCAGAATG GATGGATCCC CACCTTGGAG 
CTGGAGAACA TCTTTCCTAG CCTGGATGAC CTTATCGAGG CTCATGCTGT ATTCCTGGAC 
ACCTTGAAGC GTCTGCGCCT TGAGAATAAC TCAGTGATCC AGGAAATAGG CACCATCTTG 
CTGAACCGGT TTGACGGCAC AGAGGGCAAC TGGTTTCAGA AGATTTCATC TCGGTTCTGC 
AGCCGGCAGC CATTTGCCTT GGAACAGTTG AAAGCGAAAC AGAAGAGAGA CCCTCGATTT 
ACGCAGTTTA TTCAGGAAGC TGAGAGCAGC CCTCACTGCC GGCGGTTACA ACTAAAGGAC 
ATTATACCAT TTGAGATGCA AAGGCTGACC AAGTACCCGT TGCTGCTGCA GAACATCGCA 
AATCTAACTG AGGAAGACGA GGAGAAGCAG AGAGTGCAGA GGGCAGCAGA GTCTTGCAGG 
CAGATCCTAA ACCATGTTAA CCAGGAGGTG CGCGAGATGG AGAATAAGAT GAAACTTGCA 
GATTACCAAA GACGCCTGGA TGTTTCCAAC CTGAGGCAAA TCAACGACCC CTTATTTAAT 
GAATTTAAGA ACATTGATAT CAGCAAAAAG AATTTGATAC ATGAAGGATC TTTGACATGG 
AAAGTGAGCA GGGACAAGGC TTTTGATGTC CATGTCCTCC TCCTGGATGA TCTCTTGATG 
CTGCTGCTGA AACAAGATGA GCGGCTGGTA CTGAAGTGCC AGAGTCGGAC TATTATTCCT 
GCTCCAGACG GGAAGCTCAT GCTGAGCCCA ATCATCAAGC TGAATATGGC CATGGTCAGA 
GAGGTGGCTA CAGATAAGAA TGCCTTTTTT GTGATTTTCA ACTGGGAGGA TCGAGCACAG 
TTCTATGAAC TTGTGGCTCC AACACCCGCT GAGAAGAAAA ACTGGTTTTT ACTGATCAGC 
AACACTACAG GCTTATTGGA ACCCCCAGCT TCTTCCAAAC AGAGACACAG ATCTATCACT 
CCTGGCACAA TGCCGCAGAG CCCCTCCGAC TTTGCAGATC AGCTGCTGAG CAGCTCAGAA 
AATGGAGGCT CCCTGAAAGA TTCAGTATCC AAAGATGAAA AGGAAGAGTC GGTGGAGGAT 
GCATTGGAAG GTCAGGAAGA GAAGGACTTC AGCGGCCTTA CAGAATACCT CCTTGCCAGC 
AACATTGATC TGAACCAAGA TGCAGCATCT GGAGGAGGCA ATGTTGCCAG CGAGGCCTTG 
GAAGAAGTGT CGGTGCTAAA ACGCCTGCTA GTGCGCAATA TCCGCCTCAG TGTGGATGCA 
GACACGCTCT CAACCCTGGA GACCTGGTCA GCGGGGGAAG AACCTGCACT GGAGGCTTCT 
GAACAGGGAA GGGCCTCCAA TGGGGATCGA GAGGCAGAGG ATTCTGAAAT GGAATCTACA 
CAACAGGGAG AGAATGGCAG AAAGCAGAGT TCTGCAGATG TTGCAGAGTC CCTGTGCGCA 
CCCATTGTAC TGTCCAAAGA ACAGTCGGAG GAGGTGAAGA GGCACATTCT GTCACTGGAG 
CAGAGGATCC AGCAGCTTAA GCTGATCGAG GAAGATTACA ACAACCTGCA CAGAACAATC 
GCCAAATTCA CTGTACCGAG GAGCAGCTTT ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgef1 ( XM_012967446.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012822900.1 Xenopus tropicalis Rho guanine nucleotide exchange factor 1 (arhgef1), transcript variant X8, mRNA.

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CloneID	OXa29996
Clone ID Related Accession (Same CDS sequence)	XM_012967446.2
Accession Version	XM_012967446.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3198bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho guanine nucleotide exchange factor 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012967446.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATCCGG ACGATGGCCA TTTTGGGGGT TTGTACTCCA AGGGGTCCAT GTCGATCATT 
GGAGCAGAGG ATGAGGATTT TGAGAATGAC ATTGAACCGA ATATGGTTGA TGAGAGCAGT 
TACTTCCGAA GCATTGAGCT AGTGAAAGAA CACCCGGCCT ACATTATGGT GTTCTTGCAG 
CACGTGGTTC TTCAGTTTGA CCCCAGCCCA CTGCTCTGTT TCCTGCATGC GGAGATGCTA 
AAGAACCTTT CAGCCAAAGA GCTTAAGAAA GTCTTCATGG ATTTTCACTA CACATTTCTG 
ATGAAAGGAG CGGTACTGCA TGTCAGTGTG CCAAACAGCG TATCCTTTGA ACTGGATCGG 
ACAAGGCCTG AGATGATCCC AGAAGAAACC CAGAGAAAAT ATGTCAAAGA CATTCAGAAG 
CACCAAACTG AGGCCATTTC CCAACAGCTG GAGGACTTTA GGGCCAAGCG AGTACTTGGT 
ATGACCCCTG GGGAACTGGA GCTGAGCGAG CTTGAATCCT ATCGAACCAA CGACCGCAGT 
ATCCTGGAAG CCAAAGAGAA GCAGTTGGCA GAGCAGCTCC TGGCACGGCT GGATGAGATG 
CACCTAACTA TTGCTCCAGA CGAGGAAAAA AGCTCTGCCA TCTTTTGTGC CATTGTAGCC 
TACATGAAGC ACCTGGGGGT GAAGACCAAG GCGGCGGATA GCAAAAAATC TAAAGGAATC 
TTCTTCAGGA AAAAGGTTTC TACAAATAAA AAGGATGAGC CTCTGAAAAC AAAGAAAACA 
TTCAGCATCT TGGATCCTAT GCGCTGGAAC CGAAATGATT CTCACTATGT TGACAGCAGA 
CCATCCAAAG TTGAGCCCGA AGCTGAGAAG CCAAACCCGG ACAGAAGGGT CAGCCAGGGG 
CAGAGAACTT TTGATAGGAG TGACAGCAGC ACAGCGAAAT CCAAGAACAG CGAAGGGCCT 
GATTCTGTGG TGTCTGTAAA TATCATCCCC CCTCCTAATG AGCCCAATGA TCCTGACCCA 
GCAGTGACTG ATGGGGCATT GTCTAATGAT GCATCTGAGG TCCTCAAAGG ATCCCCCACC 
ACTGAGCATT GCCCAGCAGA CCAGACTGAG GAAAGTGCAG AAAATGAAAG ACGAAAGAAA 
AGGCTCTCTG GTAAGCTGGG CCGCTCGGAG AGCCTACGAG TTTACGAGCG CAAGCGTTCC 
CAGCGCGGCT CCTCCAAAGC CAAGCAGACA CGTTCCCGTA GCGACGTTGA CCTGGAGGCA 
GCTGCCCGAG CCAACGACTT GCAGGGCACC ACTGGCAAGC TTGGGCCAAA GCCTGGCAAC 
TACACCCCAG AGCCTGGAGG GAGTTCAGAG GGCCCGCTGC CCTTTCTGCT TCCCCAGTCT 
GAGGAGACTG AGCCGCGCGT TCAGGAGGCC GAGAGCGACC CCCCAAACTG GAGGGAGCTC 
GTCCCCCCTG AAATACTCCT CAGGCTGAAG AAAAGCGAAA TCAAGCGGCA AGAGGTCATC 
AATGAGCTCT TCTTTACAGA GCACGCTCAT GTACGGATGC TTCGTGTGTT GCATGATGTC 
TTCTACATCC CCATGCAGAA TGGATGGATC CCCACCTTGG AGCTGGAGAA CATCTTTCCT 
AGCCTGGATG ACCTTATCGA GGCTCATGCT GTATTCCTGG ACACCTTGAA GCGTCTGCGC 
CTTGAGAATA ACTCAGTGAT CCAGGAAATA GGCACCATCT TGCTGAACCG GTTTGACGGC 
ACAGAGGGCA ACTGGTTTCA GAAGATTTCA TCTCGGTTCT GCAGCCGGCA GCCATTTGCC 
TTGGAACAGT TGAAAGCGAA ACAGAAGAGA GACCCTCGAT TTACGCAGTT TATTCAGGAA 
GCTGAGAGCA GCCCTCACTG CCGGCGGTTA CAACTAAAGG ACATTATACC ATTTGAGATG 
CAAAGGCTGA CCAAGTACCC GTTGCTGCTG CAGAACATCG CAAATCTAAC TGAGGAAGAC 
GAGGAGAAGC AGAGAGTGCA GAGGGCAGCA GAGTCTTGCA GGCAGATCCT AAACCATGTT 
AACCAGGAGG TGCGCGAGAT GGAGAATAAG ATGAAACTTG CAGATTACCA AAGACGCCTG 
GATGTTTCCA ACCTGAGGCA AATCAACGAC CCCTTATTTA ATGAATTTAA GAACATTGAT 
ATCAGCAAAA AGAATTTGAT ACATGAAGGA TCTTTGACAT GGAAAGTGAG CAGGGACAAG 
GCTTTTGATG TCCATGTCCT CCTCCTGGAT GATCTCTTGA TGCTGCTGCT GAAACAAGAT 
GAGCGGCTGG TACTGAAGTG CCAGAGTCGG ACTATTATTC CTGCTCCAGA CGGGAAGCTC 
ATGCTGAGCC CAATCATCAA GCTGAATATG GCCATGGTCA GAGAGGTGGC TACAGATAAG 
AATGCCTTTT TTGTGATTTT CAACTGGGAG GATCGAGCAC AGTTCTATGA ACTTGTGGCT 
CCAACACCCG CTGAGAAGAA AAACTGGTTT TTACTGATCA GCAACACTAC AGGCTTATTG 
GAACCCCCAG CTTCTTCCAA ACAGAGACAC AGATCTATCA CTCCTGGCAC AATGCCGCAG 
AGCCCCTCCG ACTTTGCAGA TCAGCTGCTG AGCAGCTCAG AAAATGGAGG CTCCCTGAAA 
GATTCAGTAT CCAAAGATGA AAAGGAAGAG TCGGTGGAGG ATGCATTGGA AGGTCAGGAA 
GAGAAGGACT TCAGCGGCCT TACAGAATAC CTCCTTGCCA GCAACATTGA TCTGAACCAA 
GATGCAGCAT CTGGAGGAGG CAATGTTGCC AGCGAGGCCT TGGAAGAAGT GTCGGTGCTA 
AAACGCCTGC TAGTGCGCAA TATCCGCCTC AGTGTGGATG CAGACACGCT CTCAACCCTG 
GAGACCTGGT CAGCGGGGGA AGAACCTGCA CTGGAGGCTT CTGAACAGGG AAGGGCCTCC 
AATGGGGATC GAGAGGCAGA GGATTCTGAA ATGGAATCTA CACAACAGGG AGAGAATGGC 
AGAAAGCAGA GTTCTGCAGA TGTTGCAGAG TCCCTGTGCG CACCCATTGT ACTGTCCAAA 
GAACAGTCGG AGGAGGTGAA GAGGCACATT CTGTCACTGG AGCAGAGGAT CCAGCAGCTT 
AAGCTGATCG AGGAAGATTA CAACAACCTG CACAGAACAA TCGCCAAATT CACTGTACCG 
AGGAGCAGCT TTACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012822900.1
CDS	333..3530
Translation	MDPDDGHFGGLYSKGSMSIIGAEDEDFENDIEPNMVDESSYFRSIELVKEHPAYIMVFLQHVVLQFDPSPLLCFLHAEMLKNLSAKELKKVFMDFHYTFLMKGAVLHVSVPNSVSFELDRTRPEMIPEETQRKYVKDIQKHQTEAISQQLEDFRAKRVLGMTPGELELSELESYRTNDRSILEAKEKQLAEQLLARLDEMHLTIAPDEEKSSAIFCAIVAYMKHLGVKTKAADSKKSKGIFFRKKVSTNKKDEPLKTKKTFSILDPMRWNRNDSHYVDSRPSKVEPEAEKPNPDRRVSQGQRTFDRSDSSTAKSKNSEGPDSVVSVNIIPPPNEPNDPDPAVTDGALSNDASEVLKGSPTTEHCPADQTEESAENERRKKRLSGKLGRSESLRVYERKRSQRGSSKAKQTRSRSDVDLEAAARANDLQGTTGKLGPKPGNYTPEPGGSSEGPLPFLLPQSEETEPRVQEAESDPPNWRELVPPEILLRLKKSEIKRQEVINELFFTEHAHVRMLRVLHDVFYIPMQNGWIPTLELENIFPSLDDLIEAHAVFLDTLKRLRLENNSVIQEIGTILLNRFDGTEGNWFQKISSRFCSRQPFALEQLKAKQKRDPRFTQFIQEAESSPHCRRLQLKDIIPFEMQRLTKYPLLLQNIANLTEEDEEKQRVQRAAESCRQILNHVNQEVREMENKMKLADYQRRLDVSNLRQINDPLFNEFKNIDISKKNLIHEGSLTWKVSRDKAFDVHVLLLDDLLMLLLKQDERLVLKCQSRTIIPAPDGKLMLSPIIKLNMAMVREVATDKNAFFVIFNWEDRAQFYELVAPTPAEKKNWFLLISNTTGLLEPPASSKQRHRSITPGTMPQSPSDFADQLLSSSENGGSLKDSVSKDEKEESVEDALEGQEEKDFSGLTEYLLASNIDLNQDAASGGGNVASEALEEVSVLKRLLVRNIRLSVDADTLSTLETWSAGEEPALEASEQGRASNGDREAEDSEMESTQQGENGRKQSSADVAESLCAPIVLSKEQSEEVKRHILSLEQRIQQLKLIEEDYNNLHRTIAKFTVPRSSFT

Target ORF information:

RefSeq Version	XM_012967446.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho guanine nucleotide exchange factor 1 (arhgef1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012967446.2

ORF Insert Sequence:

ATGGATCCGG ACGATGGCCA TTTTGGGGGT TTGTACTCCA AGGGGTCCAT GTCGATCATT 
GGAGCAGAGG ATGAGGATTT TGAGAATGAC ATTGAACCGA ATATGGTTGA TGAGAGCAGT 
TACTTCCGAA GCATTGAGCT AGTGAAAGAA CACCCGGCCT ACATTATGGT GTTCTTGCAG 
CACGTGGTTC TTCAGTTTGA CCCCAGCCCA CTGCTCTGTT TCCTGCATGC GGAGATGCTA 
AAGAACCTTT CAGCCAAAGA GCTTAAGAAA GTCTTCATGG ATTTTCACTA CACATTTCTG 
ATGAAAGGAG CGGTACTGCA TGTCAGTGTG CCAAACAGCG TATCCTTTGA ACTGGATCGG 
ACAAGGCCTG AGATGATCCC AGAAGAAACC CAGAGAAAAT ATGTCAAAGA CATTCAGAAG 
CACCAAACTG AGGCCATTTC CCAACAGCTG GAGGACTTTA GGGCCAAGCG AGTACTTGGT 
ATGACCCCTG GGGAACTGGA GCTGAGCGAG CTTGAATCCT ATCGAACCAA CGACCGCAGT 
ATCCTGGAAG CCAAAGAGAA GCAGTTGGCA GAGCAGCTCC TGGCACGGCT GGATGAGATG 
CACCTAACTA TTGCTCCAGA CGAGGAAAAA AGCTCTGCCA TCTTTTGTGC CATTGTAGCC 
TACATGAAGC ACCTGGGGGT GAAGACCAAG GCGGCGGATA GCAAAAAATC TAAAGGAATC 
TTCTTCAGGA AAAAGGTTTC TACAAATAAA AAGGATGAGC CTCTGAAAAC AAAGAAAACA 
TTCAGCATCT TGGATCCTAT GCGCTGGAAC CGAAATGATT CTCACTATGT TGACAGCAGA 
CCATCCAAAG TTGAGCCCGA AGCTGAGAAG CCAAACCCGG ACAGAAGGGT CAGCCAGGGG 
CAGAGAACTT TTGATAGGAG TGACAGCAGC ACAGCGAAAT CCAAGAACAG CGAAGGGCCT 
GATTCTGTGG TGTCTGTAAA TATCATCCCC CCTCCTAATG AGCCCAATGA TCCTGACCCA 
GCAGTGACTG ATGGGGCATT GTCTAATGAT GCATCTGAGG TCCTCAAAGG ATCCCCCACC 
ACTGAGCATT GCCCAGCAGA CCAGACTGAG GAAAGTGCAG AAAATGAAAG ACGAAAGAAA 
AGGCTCTCTG GTAAGCTGGG CCGCTCGGAG AGCCTACGAG TTTACGAGCG CAAGCGTTCC 
CAGCGCGGCT CCTCCAAAGC CAAGCAGACA CGTTCCCGTA GCGACGTTGA CCTGGAGGCA 
GCTGCCCGAG CCAACGACTT GCAGGGCACC ACTGGCAAGC TTGGGCCAAA GCCTGGCAAC 
TACACCCCAG AGCCTGGAGG GAGTTCAGAG GGCCCGCTGC CCTTTCTGCT TCCCCAGTCT 
GAGGAGACTG AGCCGCGCGT TCAGGAGGCC GAGAGCGACC CCCCAAACTG GAGGGAGCTC 
GTCCCCCCTG AAATACTCCT CAGGCTGAAG AAAAGCGAAA TCAAGCGGCA AGAGGTCATC 
AATGAGCTCT TCTTTACAGA GCACGCTCAT GTACGGATGC TTCGTGTGTT GCATGATGTC 
TTCTACATCC CCATGCAGAA TGGATGGATC CCCACCTTGG AGCTGGAGAA CATCTTTCCT 
AGCCTGGATG ACCTTATCGA GGCTCATGCT GTATTCCTGG ACACCTTGAA GCGTCTGCGC 
CTTGAGAATA ACTCAGTGAT CCAGGAAATA GGCACCATCT TGCTGAACCG GTTTGACGGC 
ACAGAGGGCA ACTGGTTTCA GAAGATTTCA TCTCGGTTCT GCAGCCGGCA GCCATTTGCC 
TTGGAACAGT TGAAAGCGAA ACAGAAGAGA GACCCTCGAT TTACGCAGTT TATTCAGGAA 
GCTGAGAGCA GCCCTCACTG CCGGCGGTTA CAACTAAAGG ACATTATACC ATTTGAGATG 
CAAAGGCTGA CCAAGTACCC GTTGCTGCTG CAGAACATCG CAAATCTAAC TGAGGAAGAC 
GAGGAGAAGC AGAGAGTGCA GAGGGCAGCA GAGTCTTGCA GGCAGATCCT AAACCATGTT 
AACCAGGAGG TGCGCGAGAT GGAGAATAAG ATGAAACTTG CAGATTACCA AAGACGCCTG 
GATGTTTCCA ACCTGAGGCA AATCAACGAC CCCTTATTTA ATGAATTTAA GAACATTGAT 
ATCAGCAAAA AGAATTTGAT ACATGAAGGA TCTTTGACAT GGAAAGTGAG CAGGGACAAG 
GCTTTTGATG TCCATGTCCT CCTCCTGGAT GATCTCTTGA TGCTGCTGCT GAAACAAGAT 
GAGCGGCTGG TACTGAAGTG CCAGAGTCGG ACTATTATTC CTGCTCCAGA CGGGAAGCTC 
ATGCTGAGCC CAATCATCAA GCTGAATATG GCCATGGTCA GAGAGGTGGC TACAGATAAG 
AATGCCTTTT TTGTGATTTT CAACTGGGAG GATCGAGCAC AGTTCTATGA ACTTGTGGCT 
CCAACACCCG CTGAGAAGAA AAACTGGTTT TTACTGATCA GCAACACTAC AGGCTTATTG 
GAACCCCCAG CTTCTTCCAA ACAGAGACAC AGATCTATCA CTCCTGGCAC AATGCCGCAG 
AGCCCCTCCG ACTTTGCAGA TCAGCTGCTG AGCAGCTCAG AAAATGGAGG CTCCCTGAAA 
GATTCAGTAT CCAAAGATGA AAAGGAAGAG TCGGTGGAGG ATGCATTGGA AGGTCAGGAA 
GAGAAGGACT TCAGCGGCCT TACAGAATAC CTCCTTGCCA GCAACATTGA TCTGAACCAA 
GATGCAGCAT CTGGAGGAGG CAATGTTGCC AGCGAGGCCT TGGAAGAAGT GTCGGTGCTA 
AAACGCCTGC TAGTGCGCAA TATCCGCCTC AGTGTGGATG CAGACACGCT CTCAACCCTG 
GAGACCTGGT CAGCGGGGGA AGAACCTGCA CTGGAGGCTT CTGAACAGGG AAGGGCCTCC 
AATGGGGATC GAGAGGCAGA GGATTCTGAA ATGGAATCTA CACAACAGGG AGAGAATGGC 
AGAAAGCAGA GTTCTGCAGA TGTTGCAGAG TCCCTGTGCG CACCCATTGT ACTGTCCAAA 
GAACAGTCGG AGGAGGTGAA GAGGCACATT CTGTCACTGG AGCAGAGGAT CCAGCAGCTT 
AAGCTGATCG AGGAAGATTA CAACAACCTG CACAGAACAA TCGCCAAATT CACTGTACCG 
AGGAGCAGCT TTACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	arhgef1
Entrez Gene ID	100037905
Full Name	Rho guanine nucleotide exchange factor 1
General protein information	Preferred Names Rho guanine nucleotide exchange factor 1 Names rho guanine nucleotide exchange factor 1 Rho guanine nucleotide exchange factor (GEF) 1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

Xenopus tropicalis (tropical clawed frog)	arhgef1	XP_002941974.2
Canis lupus familiaris (dog)	ARHGEF1	XP_005616572.1
Canis lupus familiaris (dog)	LOC100856005	XP_003640357.2
Pan troglodytes (chimpanzee)	ARHGEF1	XP_003953551.1
Mus musculus (house mouse)	Arhgef1	NP_032514.1
Rattus norvegicus (Norway rat)	Arhgef1	NP_067726.3
Homo sapiens (human)	ARHGEF1	NP_945353.1
Bos taurus (cattle)	ARHGEF1	XP_005219451.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

arhgef1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

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