ARHGEF1 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_010823706.2	XP_010822008.1	rho guanine nucleotide exchange factor 1 isoform X3
XM_005195333.3	XP_005195390.1	rho guanine nucleotide exchange factor 1 isoform X3
XM_010815115.2	XP_010813417.1	rho guanine nucleotide exchange factor 1 isoform X1
XM_010815116.2	XP_010813418.1	rho guanine nucleotide exchange factor 1 isoform X2
XM_005219394.3	XP_005219451.1	rho guanine nucleotide exchange factor 1 isoform X3
XM_010815120.2	XP_010813422.1	rho guanine nucleotide exchange factor 1 isoform X3
XM_010815115.3	XP_010813417.1	rho guanine nucleotide exchange factor 1 isoform X5
XM_024979118.1	XP_024834886.1	rho guanine nucleotide exchange factor 1 isoform X8
XM_024979121.1	XP_024834889.1	rho guanine nucleotide exchange factor 1 isoform X10
XM_010815123.2	XP_010813425.1	rho guanine nucleotide exchange factor 1 isoform X4
XM_024979112.1	XP_024834880.1	rho guanine nucleotide exchange factor 1 isoform X2
XM_024979120.1	XP_024834888.1	rho guanine nucleotide exchange factor 1 isoform X10
XM_005219395.3	XP_005219452.1	rho guanine nucleotide exchange factor 1 isoform X3
XM_010815120.3	XP_010813422.2	rho guanine nucleotide exchange factor 1 isoform X1
XM_010823701.2	XP_010822003.1	rho guanine nucleotide exchange factor 1 isoform X1
XM_010823702.2	XP_010822004.1	rho guanine nucleotide exchange factor 1 isoform X2
XM_010823709.2	XP_010822011.1	rho guanine nucleotide exchange factor 1 isoform X4
XM_024979113.1	XP_024834881.1	rho guanine nucleotide exchange factor 1 isoform X3
XM_024979115.1	XP_024834883.1	rho guanine nucleotide exchange factor 1 isoform X6
XM_005219394.4	XP_005219451.1	rho guanine nucleotide exchange factor 1 isoform X7
XM_005219395.4	XP_005219452.1	rho guanine nucleotide exchange factor 1 isoform X7
XM_024979117.1	XP_024834885.1	rho guanine nucleotide exchange factor 1 isoform X8
XM_024979119.1	XP_024834887.1	rho guanine nucleotide exchange factor 1 isoform X9
XM_005195332.3	XP_005195389.1	rho guanine nucleotide exchange factor 1 isoform X3
XM_024979114.1	XP_024834882.1	rho guanine nucleotide exchange factor 1 isoform X4
XM_024979116.1	XP_024834884.1	rho guanine nucleotide exchange factor 1 isoform X6

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa225593	XM_024979117.1 Latest version!	Bos taurus Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa225593	XM_024979118.1 Latest version!	Bos taurus Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa225594	XM_024979119.1 Latest version!	Bos taurus Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa225595	XM_024979121.1 Latest version!	Bos taurus Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa225595	XM_024979120.1 Latest version!	Bos taurus Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa225589	XM_010815120.3 Latest version!	Bos taurus Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa225590	XM_024979112.1 Latest version!	Bos taurus Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa225591	XM_024979113.1 Latest version!	Bos taurus Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa225592	XM_024979114.1 Latest version!	Bos taurus Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa09522	XM_010823706.2 Latest version!	Bos taurus Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OBa09522	XM_010815120.2 Latest version!	Bos taurus Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OBa09522	XM_005195333.3 Latest version!	Bos taurus Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OBa09522	XM_005219395.4 Latest version!	Bos taurus Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OBa09522	XM_005219394.4 Latest version!	Bos taurus Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OBa09522	XM_005195332.3 Latest version!	Bos taurus Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OBa48599	XM_010815115.3 Latest version!	Bos taurus Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OBa48599	XM_010823701.2 Latest version!	Bos taurus Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OBa48600	XM_010815116.2 Latest version!	Bos taurus Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OBa48600	XM_010823702.2 Latest version!	Bos taurus Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OBa48601	XM_024979115.1 Latest version!	Bos taurus Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OBa48601	XM_024979116.1 Latest version!	Bos taurus Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OBa48603	XM_010815123.2 Latest version!	Bos taurus Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OBa48603	XM_010823709.2 Latest version!	Bos taurus Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
View Old Accession Versions >>
OBa48599	XM_010815115.2	Bos taurus Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OBa09522	XM_005219394.3	Bos taurus Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OBa09522	XM_005219395.3	Bos taurus Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
Hide Old Accession Versions >>

ARHGEF1 ( XM_024979118.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024834886.1 Bos taurus Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OBa225593
Clone ID Related Accession (Same CDS sequence)	XM_024979118.1 , XM_024979117.1
Accession Version	XM_024979118.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2904bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rho guanine nucleotide exchange factor 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCAG AAGACGTCGC CCGAGGGGCG GCCCCGGGGC CCCCCCGACC CGGCCTGGCG 
CCCGTCAGCA TCATTGGGGC AGAGGACGAG GATTTTGAGA TTGAGTTGAA GACGAACCCT 
GAGGAACAAA ACAACCAGTT CCAGAACCTG GATCAGGTGA AGCGGCGCCC GGCCCACCTC 
ATGGCCCTCC TGCAGCACGT GGCCATGCAG TTTGAGCCGG GACCCCTGCT CTGCTGCCTG 
CATGCGGACA TGCTGGGCTC CCTGGGCCCC AAGGAGTTCA AGAAAGCCTT CATCGACTTC 
TACCACAGCT TCCTGGATAA GCACTCGATT CTACGAGTGA TGGTCCCTCC TGCCGTGGCC 
TTTGAACTTG ACCGCACGCG GCCTGACCTC TTTCCTGAGG ATCTCCAGCG GCAGTTCGTG 
CAGGAGGTGG TGCAGAGCCA GCAGGTAGCC GTGGTCCGGC AGCTGGAGGA CTTCCGCTCC 
AAGAAGCTCA TGGGCATGAC ACCCTGGGAG CAGGAGCTGG TCCAGCTGGG GGCCTGGGTG 
GCGCGGGACC GTGCCAGCTA CGAGGCCCGG GAGCGGCACC TGGCTGAGCG GCAACTGACC 
CACCTGGAGG AGATGCAGCA CACCATCTCT AACGATGAGG AGAAGAGCGC CGCCGTGGTC 
AACGCCATCA GCATGTACAT GCGGCACCTT GGGGTGCGGA CCAAGAGTGG GGACAAGAAG 
TCCGGGAGGA ACTTCTTTCG GAAAAAGATG ATAGGGAACC GGCGGTCAGA TGAACCGACC 
AAGACCAAGA AAGGCCTGAG CAGCTTCCTG GATGCCGCCC GCTGGAACCG GGGAGAGCCC 
CAGGATTTTC GACACCCGAA GGTCGAAGTT GATGCTGAGA AGCCAGGCCT TACAGAACGT 
AAGGGAGGTC AGGGGGCGCC TTCCCGGGAC CGGAATCTCG GAGGCCCTGG GCAGGACACC 
CCCGGAGTTT CTCCGCACCT TCTGTCTGGG GACAGCCCTG ACCGGGAGCC AGGTGTTGAC 
GCCCTCCTGG AGGTGGGGGA CCCGCCCCCA CAGGGCCCAG CCAGCCTGGA CGCCCCAGCG 
CCGCTGGAGA GCACGGAGGA GGCTGCCGAC ACAGAAAGGC TGTCAGGGCG TCTGGGGCGC 
TCAGAGAGCC TGCGGGTGAG TGACCGCCGC CGGCCTTCCC GGGGTAGCCT CGGGGCTAAG 
GGCCGGGGTG GGGGCCGCTC CCGGAGCGAC GTGGACATGG ACCCCAGCTC CGCCACGGCC 
GTGCTTGGCC CTACCCGACG AGCCACCCCG GAGCCTGGAG ATGAGGGAGA GCCGGGCCGC 
TCAGGACTGG AGCTGGAACC AGAGGAGCCG CCTGGCTGGC GAGAGCTTGT CCCCCAGGAC 
ATTCTGCACA GCCTGCCCAA GAGCCAGGTG AAGCGGCAGG AGGTCATCAG CGAGCTCCTG 
GTGACGGAGG CTGCCCATGT GCGCATGCTC CGCGTGCTCC ACGACGTCTT CTACCAGCCC 
ATAGCGGACG GGGGCTTCTA CTCCCAGGAG GAGCTCCAGA ACATCTTCCC CAGCCTGGAC 
GAGCTCATCG AGGTGCATTC CCTATTCCTT GATTCCCTGA TGAAGCGGAG GCAGGACAGT 
GGCTACCTCA TCAAGGAGAT CGGAGATGTG CTGCTGGCCC GGTTCGATGG TGCCGAGGGC 
TCGTGGTTCC AGAAAATCTC CTCCCGCTTC TGTAGCCGCC AGTCGTTCGC CTTAGAGCAG 
CTCAAAGCCA AACAGCGCAA GGAGCCCCGG TTCTGTACCT TCGTGCAGGA GGCCGAGAGC 
CGGCCCCGGT GCCGCCGGCT GCAGCTGAAG GACATGATCC CCACCGAGAT GCAGCGTCTG 
ACCAAGTACC CACTGCTTCT GCAGAGCATC GAGCAGAACA CAGAAGAGCC GGTGGAACGG 
GAGAAAGTGG AGCGGGCAGC CGAGTGCTGC CGGGAAATTC TGCAACACGT CAACCTTGCC 
GTGCGGGACA TGGAGGACCT GCTGCGGCTC AAAGATTATC AGAGGCGTCT GGATTTGACC 
CACCTGCGGC AGAGCAACGA CCCCATGCTG AGCGAGTTCA AGAACCTAGA CATCACCAAG 
AAGAGGTTGA TCTACGAGGG CCCACTGACG TGGCGGGTGA CGAAGGACAA GGCTGTGGAG 
GTCCACGTGC TGCTGCTGGA TGACCTGCTG CTGCTGCTTC AGCGCCAGGA CGAGCGGTTC 
TTGCTTAAGT CGCACAGCCG GACGCTGACG CCCACGCCCG ATGGCAAGAC CATGCTGCGG 
CCAGTGCTGC GGCTCACCTC CGCCATGACC CGCGAGGTGG CCACCGATCA CAAAGCCTTC 
TATGTCATTT TTACCTGGGA CCAGGAGGCC CAGATATACG AGCTGGTGGC GCAGACGGTG 
TCTGAGCGGA GGATCTGGTG TACCCTGATC ACCGAGACCG CTGGATGCCT GAAGGTCCCC 
TCCCGCACCT CCCGACCCAA ACACCGGCCC GGCCCCAGCA GCACTCGTGA ACCCCTGCTT 
AGCAGCTCCG AAAACGGCAA TGGCAGCCGA GAGATGCCCC CGGCAGATGG CCGGATGGAG 
AGACTCTTCA ACTCCCTCCT GCCCTTCTGC AGACAAGGCC TCGAGGGCCA GCTCGCCGCC 
AAGGCCCTTC AGAAAGTGCT GACCCTGAAA CAGCTCCTGT TGCCTTCGGA GGAAGACAGC 
GGGGCAGGGC CTCCCCTCGA AGGGGATGGG GTCCCGGGGG GCAGCCCCTT GAGCCCAACG 
CAGCCCCAGG AAATTCGGGA GGAGCTGCTC AGCCTGGAGG AGACCATTAA ACAGCTAGAG 
GAGGTGGAGG AGGAATTTTG CCGCCTGAGA CTCCTCCTGT CTCAGCTTGG GGAGAACACC 
GTCCCCCAGT CTGGCTGTAC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024834886.1
CDS	150..3053
Translation	MEAEDVARGAAPGPPRPGLAPVSIIGAEDEDFEIELKTNPEEQNNQFQNLDQVKRRPAHLMALLQHVAMQFEPGPLLCCLHADMLGSLGPKEFKKAFIDFYHSFLDKHSILRVMVPPAVAFELDRTRPDLFPEDLQRQFVQEVVQSQQVAVVRQLEDFRSKKLMGMTPWEQELVQLGAWVARDRASYEARERHLAERQLTHLEEMQHTISNDEEKSAAVVNAISMYMRHLGVRTKSGDKKSGRNFFRKKMIGNRRSDEPTKTKKGLSSFLDAARWNRGEPQDFRHPKVEVDAEKPGLTERKGGQGAPSRDRNLGGPGQDTPGVSPHLLSGDSPDREPGVDALLEVGDPPPQGPASLDAPAPLESTEEAADTERLSGRLGRSESLRVSDRRRPSRGSLGAKGRGGGRSRSDVDMDPSSATAVLGPTRRATPEPGDEGEPGRSGLELEPEEPPGWRELVPQDILHSLPKSQVKRQEVISELLVTEAAHVRMLRVLHDVFYQPIADGGFYSQEELQNIFPSLDELIEVHSLFLDSLMKRRQDSGYLIKEIGDVLLARFDGAEGSWFQKISSRFCSRQSFALEQLKAKQRKEPRFCTFVQEAESRPRCRRLQLKDMIPTEMQRLTKYPLLLQSIEQNTEEPVEREKVERAAECCREILQHVNLAVRDMEDLLRLKDYQRRLDLTHLRQSNDPMLSEFKNLDITKKRLIYEGPLTWRVTKDKAVEVHVLLLDDLLLLLQRQDERFLLKSHSRTLTPTPDGKTMLRPVLRLTSAMTREVATDHKAFYVIFTWDQEAQIYELVAQTVSERRIWCTLITETAGCLKVPSRTSRPKHRPGPSSTREPLLSSSENGNGSREMPPADGRMERLFNSLLPFCRQGLEGQLAAKALQKVLTLKQLLLPSEEDSGAGPPLEGDGVPGGSPLSPTQPQEIREELLSLEETIKQLEEVEEEFCRLRLLLSQLGENTVPQSGCT

Target ORF information:

RefSeq Version	XM_024979118.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024979118.1

ORF Insert Sequence:

ATGGAGGCAG AAGACGTCGC CCGAGGGGCG GCCCCGGGGC CCCCCCGACC CGGCCTGGCG 
CCCGTCAGCA TCATTGGGGC AGAGGACGAG GATTTTGAGA TTGAGTTGAA GACGAACCCT 
GAGGAACAAA ACAACCAGTT CCAGAACCTG GATCAGGTGA AGCGGCGCCC GGCCCACCTC 
ATGGCCCTCC TGCAGCACGT GGCCATGCAG TTTGAGCCGG GACCCCTGCT CTGCTGCCTG 
CATGCGGACA TGCTGGGCTC CCTGGGCCCC AAGGAGTTCA AGAAAGCCTT CATCGACTTC 
TACCACAGCT TCCTGGATAA GCACTCGATT CTACGAGTGA TGGTCCCTCC TGCCGTGGCC 
TTTGAACTTG ACCGCACGCG GCCTGACCTC TTTCCTGAGG ATCTCCAGCG GCAGTTCGTG 
CAGGAGGTGG TGCAGAGCCA GCAGGTAGCC GTGGTCCGGC AGCTGGAGGA CTTCCGCTCC 
AAGAAGCTCA TGGGCATGAC ACCCTGGGAG CAGGAGCTGG TCCAGCTGGG GGCCTGGGTG 
GCGCGGGACC GTGCCAGCTA CGAGGCCCGG GAGCGGCACC TGGCTGAGCG GCAACTGACC 
CACCTGGAGG AGATGCAGCA CACCATCTCT AACGATGAGG AGAAGAGCGC CGCCGTGGTC 
AACGCCATCA GCATGTACAT GCGGCACCTT GGGGTGCGGA CCAAGAGTGG GGACAAGAAG 
TCCGGGAGGA ACTTCTTTCG GAAAAAGATG ATAGGGAACC GGCGGTCAGA TGAACCGACC 
AAGACCAAGA AAGGCCTGAG CAGCTTCCTG GATGCCGCCC GCTGGAACCG GGGAGAGCCC 
CAGGATTTTC GACACCCGAA GGTCGAAGTT GATGCTGAGA AGCCAGGCCT TACAGAACGT 
AAGGGAGGTC AGGGGGCGCC TTCCCGGGAC CGGAATCTCG GAGGCCCTGG GCAGGACACC 
CCCGGAGTTT CTCCGCACCT TCTGTCTGGG GACAGCCCTG ACCGGGAGCC AGGTGTTGAC 
GCCCTCCTGG AGGTGGGGGA CCCGCCCCCA CAGGGCCCAG CCAGCCTGGA CGCCCCAGCG 
CCGCTGGAGA GCACGGAGGA GGCTGCCGAC ACAGAAAGGC TGTCAGGGCG TCTGGGGCGC 
TCAGAGAGCC TGCGGGTGAG TGACCGCCGC CGGCCTTCCC GGGGTAGCCT CGGGGCTAAG 
GGCCGGGGTG GGGGCCGCTC CCGGAGCGAC GTGGACATGG ACCCCAGCTC CGCCACGGCC 
GTGCTTGGCC CTACCCGACG AGCCACCCCG GAGCCTGGAG ATGAGGGAGA GCCGGGCCGC 
TCAGGACTGG AGCTGGAACC AGAGGAGCCG CCTGGCTGGC GAGAGCTTGT CCCCCAGGAC 
ATTCTGCACA GCCTGCCCAA GAGCCAGGTG AAGCGGCAGG AGGTCATCAG CGAGCTCCTG 
GTGACGGAGG CTGCCCATGT GCGCATGCTC CGCGTGCTCC ACGACGTCTT CTACCAGCCC 
ATAGCGGACG GGGGCTTCTA CTCCCAGGAG GAGCTCCAGA ACATCTTCCC CAGCCTGGAC 
GAGCTCATCG AGGTGCATTC CCTATTCCTT GATTCCCTGA TGAAGCGGAG GCAGGACAGT 
GGCTACCTCA TCAAGGAGAT CGGAGATGTG CTGCTGGCCC GGTTCGATGG TGCCGAGGGC 
TCGTGGTTCC AGAAAATCTC CTCCCGCTTC TGTAGCCGCC AGTCGTTCGC CTTAGAGCAG 
CTCAAAGCCA AACAGCGCAA GGAGCCCCGG TTCTGTACCT TCGTGCAGGA GGCCGAGAGC 
CGGCCCCGGT GCCGCCGGCT GCAGCTGAAG GACATGATCC CCACCGAGAT GCAGCGTCTG 
ACCAAGTACC CACTGCTTCT GCAGAGCATC GAGCAGAACA CAGAAGAGCC GGTGGAACGG 
GAGAAAGTGG AGCGGGCAGC CGAGTGCTGC CGGGAAATTC TGCAACACGT CAACCTTGCC 
GTGCGGGACA TGGAGGACCT GCTGCGGCTC AAAGATTATC AGAGGCGTCT GGATTTGACC 
CACCTGCGGC AGAGCAACGA CCCCATGCTG AGCGAGTTCA AGAACCTAGA CATCACCAAG 
AAGAGGTTGA TCTACGAGGG CCCACTGACG TGGCGGGTGA CGAAGGACAA GGCTGTGGAG 
GTCCACGTGC TGCTGCTGGA TGACCTGCTG CTGCTGCTTC AGCGCCAGGA CGAGCGGTTC 
TTGCTTAAGT CGCACAGCCG GACGCTGACG CCCACGCCCG ATGGCAAGAC CATGCTGCGG 
CCAGTGCTGC GGCTCACCTC CGCCATGACC CGCGAGGTGG CCACCGATCA CAAAGCCTTC 
TATGTCATTT TTACCTGGGA CCAGGAGGCC CAGATATACG AGCTGGTGGC GCAGACGGTG 
TCTGAGCGGA GGATCTGGTG TACCCTGATC ACCGAGACCG CTGGATGCCT GAAGGTCCCC 
TCCCGCACCT CCCGACCCAA ACACCGGCCC GGCCCCAGCA GCACTCGTGA ACCCCTGCTT 
AGCAGCTCCG AAAACGGCAA TGGCAGCCGA GAGATGCCCC CGGCAGATGG CCGGATGGAG 
AGACTCTTCA ACTCCCTCCT GCCCTTCTGC AGACAAGGCC TCGAGGGCCA GCTCGCCGCC 
AAGGCCCTTC AGAAAGTGCT GACCCTGAAA CAGCTCCTGT TGCCTTCGGA GGAAGACAGC 
GGGGCAGGGC CTCCCCTCGA AGGGGATGGG GTCCCGGGGG GCAGCCCCTT GAGCCCAACG 
CAGCCCCAGG AAATTCGGGA GGAGCTGCTC AGCCTGGAGG AGACCATTAA ACAGCTAGAG 
GAGGTGGAGG AGGAATTTTG CCGCCTGAGA CTCCTCCTGT CTCAGCTTGG GGAGAACACC 
GTCCCCCAGT CTGGCTGTAC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF1 ( XM_024979117.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024834885.1 Bos taurus Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OBa225593
Clone ID Related Accession (Same CDS sequence)	XM_024979118.1 , XM_024979117.1
Accession Version	XM_024979117.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2904bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rho guanine nucleotide exchange factor 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCAG AAGACGTCGC CCGAGGGGCG GCCCCGGGGC CCCCCCGACC CGGCCTGGCG 
CCCGTCAGCA TCATTGGGGC AGAGGACGAG GATTTTGAGA TTGAGTTGAA GACGAACCCT 
GAGGAACAAA ACAACCAGTT CCAGAACCTG GATCAGGTGA AGCGGCGCCC GGCCCACCTC 
ATGGCCCTCC TGCAGCACGT GGCCATGCAG TTTGAGCCGG GACCCCTGCT CTGCTGCCTG 
CATGCGGACA TGCTGGGCTC CCTGGGCCCC AAGGAGTTCA AGAAAGCCTT CATCGACTTC 
TACCACAGCT TCCTGGATAA GCACTCGATT CTACGAGTGA TGGTCCCTCC TGCCGTGGCC 
TTTGAACTTG ACCGCACGCG GCCTGACCTC TTTCCTGAGG ATCTCCAGCG GCAGTTCGTG 
CAGGAGGTGG TGCAGAGCCA GCAGGTAGCC GTGGTCCGGC AGCTGGAGGA CTTCCGCTCC 
AAGAAGCTCA TGGGCATGAC ACCCTGGGAG CAGGAGCTGG TCCAGCTGGG GGCCTGGGTG 
GCGCGGGACC GTGCCAGCTA CGAGGCCCGG GAGCGGCACC TGGCTGAGCG GCAACTGACC 
CACCTGGAGG AGATGCAGCA CACCATCTCT AACGATGAGG AGAAGAGCGC CGCCGTGGTC 
AACGCCATCA GCATGTACAT GCGGCACCTT GGGGTGCGGA CCAAGAGTGG GGACAAGAAG 
TCCGGGAGGA ACTTCTTTCG GAAAAAGATG ATAGGGAACC GGCGGTCAGA TGAACCGACC 
AAGACCAAGA AAGGCCTGAG CAGCTTCCTG GATGCCGCCC GCTGGAACCG GGGAGAGCCC 
CAGGATTTTC GACACCCGAA GGTCGAAGTT GATGCTGAGA AGCCAGGCCT TACAGAACGT 
AAGGGAGGTC AGGGGGCGCC TTCCCGGGAC CGGAATCTCG GAGGCCCTGG GCAGGACACC 
CCCGGAGTTT CTCCGCACCT TCTGTCTGGG GACAGCCCTG ACCGGGAGCC AGGTGTTGAC 
GCCCTCCTGG AGGTGGGGGA CCCGCCCCCA CAGGGCCCAG CCAGCCTGGA CGCCCCAGCG 
CCGCTGGAGA GCACGGAGGA GGCTGCCGAC ACAGAAAGGC TGTCAGGGCG TCTGGGGCGC 
TCAGAGAGCC TGCGGGTGAG TGACCGCCGC CGGCCTTCCC GGGGTAGCCT CGGGGCTAAG 
GGCCGGGGTG GGGGCCGCTC CCGGAGCGAC GTGGACATGG ACCCCAGCTC CGCCACGGCC 
GTGCTTGGCC CTACCCGACG AGCCACCCCG GAGCCTGGAG ATGAGGGAGA GCCGGGCCGC 
TCAGGACTGG AGCTGGAACC AGAGGAGCCG CCTGGCTGGC GAGAGCTTGT CCCCCAGGAC 
ATTCTGCACA GCCTGCCCAA GAGCCAGGTG AAGCGGCAGG AGGTCATCAG CGAGCTCCTG 
GTGACGGAGG CTGCCCATGT GCGCATGCTC CGCGTGCTCC ACGACGTCTT CTACCAGCCC 
ATAGCGGACG GGGGCTTCTA CTCCCAGGAG GAGCTCCAGA ACATCTTCCC CAGCCTGGAC 
GAGCTCATCG AGGTGCATTC CCTATTCCTT GATTCCCTGA TGAAGCGGAG GCAGGACAGT 
GGCTACCTCA TCAAGGAGAT CGGAGATGTG CTGCTGGCCC GGTTCGATGG TGCCGAGGGC 
TCGTGGTTCC AGAAAATCTC CTCCCGCTTC TGTAGCCGCC AGTCGTTCGC CTTAGAGCAG 
CTCAAAGCCA AACAGCGCAA GGAGCCCCGG TTCTGTACCT TCGTGCAGGA GGCCGAGAGC 
CGGCCCCGGT GCCGCCGGCT GCAGCTGAAG GACATGATCC CCACCGAGAT GCAGCGTCTG 
ACCAAGTACC CACTGCTTCT GCAGAGCATC GAGCAGAACA CAGAAGAGCC GGTGGAACGG 
GAGAAAGTGG AGCGGGCAGC CGAGTGCTGC CGGGAAATTC TGCAACACGT CAACCTTGCC 
GTGCGGGACA TGGAGGACCT GCTGCGGCTC AAAGATTATC AGAGGCGTCT GGATTTGACC 
CACCTGCGGC AGAGCAACGA CCCCATGCTG AGCGAGTTCA AGAACCTAGA CATCACCAAG 
AAGAGGTTGA TCTACGAGGG CCCACTGACG TGGCGGGTGA CGAAGGACAA GGCTGTGGAG 
GTCCACGTGC TGCTGCTGGA TGACCTGCTG CTGCTGCTTC AGCGCCAGGA CGAGCGGTTC 
TTGCTTAAGT CGCACAGCCG GACGCTGACG CCCACGCCCG ATGGCAAGAC CATGCTGCGG 
CCAGTGCTGC GGCTCACCTC CGCCATGACC CGCGAGGTGG CCACCGATCA CAAAGCCTTC 
TATGTCATTT TTACCTGGGA CCAGGAGGCC CAGATATACG AGCTGGTGGC GCAGACGGTG 
TCTGAGCGGA GGATCTGGTG TACCCTGATC ACCGAGACCG CTGGATGCCT GAAGGTCCCC 
TCCCGCACCT CCCGACCCAA ACACCGGCCC GGCCCCAGCA GCACTCGTGA ACCCCTGCTT 
AGCAGCTCCG AAAACGGCAA TGGCAGCCGA GAGATGCCCC CGGCAGATGG CCGGATGGAG 
AGACTCTTCA ACTCCCTCCT GCCCTTCTGC AGACAAGGCC TCGAGGGCCA GCTCGCCGCC 
AAGGCCCTTC AGAAAGTGCT GACCCTGAAA CAGCTCCTGT TGCCTTCGGA GGAAGACAGC 
GGGGCAGGGC CTCCCCTCGA AGGGGATGGG GTCCCGGGGG GCAGCCCCTT GAGCCCAACG 
CAGCCCCAGG AAATTCGGGA GGAGCTGCTC AGCCTGGAGG AGACCATTAA ACAGCTAGAG 
GAGGTGGAGG AGGAATTTTG CCGCCTGAGA CTCCTCCTGT CTCAGCTTGG GGAGAACACC 
GTCCCCCAGT CTGGCTGTAC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024834885.1
CDS	267..3170
Translation	MEAEDVARGAAPGPPRPGLAPVSIIGAEDEDFEIELKTNPEEQNNQFQNLDQVKRRPAHLMALLQHVAMQFEPGPLLCCLHADMLGSLGPKEFKKAFIDFYHSFLDKHSILRVMVPPAVAFELDRTRPDLFPEDLQRQFVQEVVQSQQVAVVRQLEDFRSKKLMGMTPWEQELVQLGAWVARDRASYEARERHLAERQLTHLEEMQHTISNDEEKSAAVVNAISMYMRHLGVRTKSGDKKSGRNFFRKKMIGNRRSDEPTKTKKGLSSFLDAARWNRGEPQDFRHPKVEVDAEKPGLTERKGGQGAPSRDRNLGGPGQDTPGVSPHLLSGDSPDREPGVDALLEVGDPPPQGPASLDAPAPLESTEEAADTERLSGRLGRSESLRVSDRRRPSRGSLGAKGRGGGRSRSDVDMDPSSATAVLGPTRRATPEPGDEGEPGRSGLELEPEEPPGWRELVPQDILHSLPKSQVKRQEVISELLVTEAAHVRMLRVLHDVFYQPIADGGFYSQEELQNIFPSLDELIEVHSLFLDSLMKRRQDSGYLIKEIGDVLLARFDGAEGSWFQKISSRFCSRQSFALEQLKAKQRKEPRFCTFVQEAESRPRCRRLQLKDMIPTEMQRLTKYPLLLQSIEQNTEEPVEREKVERAAECCREILQHVNLAVRDMEDLLRLKDYQRRLDLTHLRQSNDPMLSEFKNLDITKKRLIYEGPLTWRVTKDKAVEVHVLLLDDLLLLLQRQDERFLLKSHSRTLTPTPDGKTMLRPVLRLTSAMTREVATDHKAFYVIFTWDQEAQIYELVAQTVSERRIWCTLITETAGCLKVPSRTSRPKHRPGPSSTREPLLSSSENGNGSREMPPADGRMERLFNSLLPFCRQGLEGQLAAKALQKVLTLKQLLLPSEEDSGAGPPLEGDGVPGGSPLSPTQPQEIREELLSLEETIKQLEEVEEEFCRLRLLLSQLGENTVPQSGCT

Target ORF information:

RefSeq Version	XM_024979117.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024979117.1

ORF Insert Sequence:

ATGGAGGCAG AAGACGTCGC CCGAGGGGCG GCCCCGGGGC CCCCCCGACC CGGCCTGGCG 
CCCGTCAGCA TCATTGGGGC AGAGGACGAG GATTTTGAGA TTGAGTTGAA GACGAACCCT 
GAGGAACAAA ACAACCAGTT CCAGAACCTG GATCAGGTGA AGCGGCGCCC GGCCCACCTC 
ATGGCCCTCC TGCAGCACGT GGCCATGCAG TTTGAGCCGG GACCCCTGCT CTGCTGCCTG 
CATGCGGACA TGCTGGGCTC CCTGGGCCCC AAGGAGTTCA AGAAAGCCTT CATCGACTTC 
TACCACAGCT TCCTGGATAA GCACTCGATT CTACGAGTGA TGGTCCCTCC TGCCGTGGCC 
TTTGAACTTG ACCGCACGCG GCCTGACCTC TTTCCTGAGG ATCTCCAGCG GCAGTTCGTG 
CAGGAGGTGG TGCAGAGCCA GCAGGTAGCC GTGGTCCGGC AGCTGGAGGA CTTCCGCTCC 
AAGAAGCTCA TGGGCATGAC ACCCTGGGAG CAGGAGCTGG TCCAGCTGGG GGCCTGGGTG 
GCGCGGGACC GTGCCAGCTA CGAGGCCCGG GAGCGGCACC TGGCTGAGCG GCAACTGACC 
CACCTGGAGG AGATGCAGCA CACCATCTCT AACGATGAGG AGAAGAGCGC CGCCGTGGTC 
AACGCCATCA GCATGTACAT GCGGCACCTT GGGGTGCGGA CCAAGAGTGG GGACAAGAAG 
TCCGGGAGGA ACTTCTTTCG GAAAAAGATG ATAGGGAACC GGCGGTCAGA TGAACCGACC 
AAGACCAAGA AAGGCCTGAG CAGCTTCCTG GATGCCGCCC GCTGGAACCG GGGAGAGCCC 
CAGGATTTTC GACACCCGAA GGTCGAAGTT GATGCTGAGA AGCCAGGCCT TACAGAACGT 
AAGGGAGGTC AGGGGGCGCC TTCCCGGGAC CGGAATCTCG GAGGCCCTGG GCAGGACACC 
CCCGGAGTTT CTCCGCACCT TCTGTCTGGG GACAGCCCTG ACCGGGAGCC AGGTGTTGAC 
GCCCTCCTGG AGGTGGGGGA CCCGCCCCCA CAGGGCCCAG CCAGCCTGGA CGCCCCAGCG 
CCGCTGGAGA GCACGGAGGA GGCTGCCGAC ACAGAAAGGC TGTCAGGGCG TCTGGGGCGC 
TCAGAGAGCC TGCGGGTGAG TGACCGCCGC CGGCCTTCCC GGGGTAGCCT CGGGGCTAAG 
GGCCGGGGTG GGGGCCGCTC CCGGAGCGAC GTGGACATGG ACCCCAGCTC CGCCACGGCC 
GTGCTTGGCC CTACCCGACG AGCCACCCCG GAGCCTGGAG ATGAGGGAGA GCCGGGCCGC 
TCAGGACTGG AGCTGGAACC AGAGGAGCCG CCTGGCTGGC GAGAGCTTGT CCCCCAGGAC 
ATTCTGCACA GCCTGCCCAA GAGCCAGGTG AAGCGGCAGG AGGTCATCAG CGAGCTCCTG 
GTGACGGAGG CTGCCCATGT GCGCATGCTC CGCGTGCTCC ACGACGTCTT CTACCAGCCC 
ATAGCGGACG GGGGCTTCTA CTCCCAGGAG GAGCTCCAGA ACATCTTCCC CAGCCTGGAC 
GAGCTCATCG AGGTGCATTC CCTATTCCTT GATTCCCTGA TGAAGCGGAG GCAGGACAGT 
GGCTACCTCA TCAAGGAGAT CGGAGATGTG CTGCTGGCCC GGTTCGATGG TGCCGAGGGC 
TCGTGGTTCC AGAAAATCTC CTCCCGCTTC TGTAGCCGCC AGTCGTTCGC CTTAGAGCAG 
CTCAAAGCCA AACAGCGCAA GGAGCCCCGG TTCTGTACCT TCGTGCAGGA GGCCGAGAGC 
CGGCCCCGGT GCCGCCGGCT GCAGCTGAAG GACATGATCC CCACCGAGAT GCAGCGTCTG 
ACCAAGTACC CACTGCTTCT GCAGAGCATC GAGCAGAACA CAGAAGAGCC GGTGGAACGG 
GAGAAAGTGG AGCGGGCAGC CGAGTGCTGC CGGGAAATTC TGCAACACGT CAACCTTGCC 
GTGCGGGACA TGGAGGACCT GCTGCGGCTC AAAGATTATC AGAGGCGTCT GGATTTGACC 
CACCTGCGGC AGAGCAACGA CCCCATGCTG AGCGAGTTCA AGAACCTAGA CATCACCAAG 
AAGAGGTTGA TCTACGAGGG CCCACTGACG TGGCGGGTGA CGAAGGACAA GGCTGTGGAG 
GTCCACGTGC TGCTGCTGGA TGACCTGCTG CTGCTGCTTC AGCGCCAGGA CGAGCGGTTC 
TTGCTTAAGT CGCACAGCCG GACGCTGACG CCCACGCCCG ATGGCAAGAC CATGCTGCGG 
CCAGTGCTGC GGCTCACCTC CGCCATGACC CGCGAGGTGG CCACCGATCA CAAAGCCTTC 
TATGTCATTT TTACCTGGGA CCAGGAGGCC CAGATATACG AGCTGGTGGC GCAGACGGTG 
TCTGAGCGGA GGATCTGGTG TACCCTGATC ACCGAGACCG CTGGATGCCT GAAGGTCCCC 
TCCCGCACCT CCCGACCCAA ACACCGGCCC GGCCCCAGCA GCACTCGTGA ACCCCTGCTT 
AGCAGCTCCG AAAACGGCAA TGGCAGCCGA GAGATGCCCC CGGCAGATGG CCGGATGGAG 
AGACTCTTCA ACTCCCTCCT GCCCTTCTGC AGACAAGGCC TCGAGGGCCA GCTCGCCGCC 
AAGGCCCTTC AGAAAGTGCT GACCCTGAAA CAGCTCCTGT TGCCTTCGGA GGAAGACAGC 
GGGGCAGGGC CTCCCCTCGA AGGGGATGGG GTCCCGGGGG GCAGCCCCTT GAGCCCAACG 
CAGCCCCAGG AAATTCGGGA GGAGCTGCTC AGCCTGGAGG AGACCATTAA ACAGCTAGAG 
GAGGTGGAGG AGGAATTTTG CCGCCTGAGA CTCCTCCTGT CTCAGCTTGG GGAGAACACC 
GTCCCCCAGT CTGGCTGTAC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF1 ( XM_024979119.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024834887.1 Bos taurus Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OBa225594
Clone ID Related Accession (Same CDS sequence)	XM_024979119.1
Accession Version	XM_024979119.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2763bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rho guanine nucleotide exchange factor 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATTCT CCAGCCCGGC AGTGCCCATG GAGGCAGAAG ACGTCGCCCG AGGGGCGGCC 
CCGGGGCCCC CCCGACCCGG CCTGGCGCCC GTCAGCATCA TTGGGGCAGA GGACGAGGAT 
TTTGAGATTG AGTTGAAGAC GAACCCTGAG GAACAAAACA ACCAGTTCCA GAACCTGGAT 
CAGGTGAAGC GGCGCCCGGC CCACCTCATG GCCCTCCTGC AGCACGTGGC CATGCAGTTT 
GAGCCGGGAC CCCTGCTCTG CTGCCTGCAT GCGGACATGC TGGGCTCCCT GGGCCCCAAG 
GAGTTCAAGA AAGCCTTCAT CGACTTCTAC CACAGCTTCC TGGATAAGCA CTCGATTCTA 
CGAGTGATGG TCCCTCCTGC CGTGGCCTTT GAACTTGACC GCACGCGGCC TGACCTCTTT 
CCTGAGGATC TCCAGCGGCA GTTCGTGCAG GAGGTGGTGC AGAGCCAGCA GGTAGCCGTG 
GTCCGGCAGC TGGAGGACTT CCGCTCCAAG AAGCTCATGG GCATGACACC CTGGGAGCAG 
GAGCTGGTCC AGCTGGGGGC CTGGGTGGCG CGGGACCGTG CCAGCTACGA GGCCCGGGAG 
CGGCACCTGG CTGAGCGGCA ACTGACCCAC CTGGAGGAGA TGCAGCACAC CATCTCTAAC 
GATGAGGAGA AGAGCGCCGC CGTGGTCAAC GCCATCAGCA TGTACATGCG GCACCTTGGG 
GTGCGGACCA AGAGTGGGGA CAAGAAGTCC GGGAGGAACT TCTTTCGGAA AAAGATGATA 
GGGAACCGGC GGTCAGATGA ACCGACCAAG ACCAAGAAAG GCCTGAGCAG CTTCCTGGAT 
GCCGCCCGCT GGAACCGGGG AGAGCCCCAG GATTTTCGAC ACCCGAAGGT CGAAGTTGAT 
GCTGAGAAGC CAGGCCTTAC AGAACGTAAG GGAGGTCAGG GGGCGCCTTC CCGGGACCGG 
AATCTCGGAG GCCCTGGGCA GGACACCCCC GGAGTTTCTC CGCACCTTCT GTCTGGGGAC 
AGCCCTGACC GGGAGCCAGG TGTTGACGCC CTCCTGGAGG TGGGGGACCC GCCCCCACAG 
GGCCCAGCCA GCCTGGACGC CCCAGCGCCG CTGGAGAGCA CGGAGGAGGC TGCCGACACA 
GAAAGCCCGG AGCCTGGAGA TGAGGGAGAG CCGGGCCGCT CAGGACTGGA GCTGGAACCA 
GAGGAGCCGC CTGGCTGGCG AGAGCTTGTC CCCCAGGACA TTCTGCACAG CCTGCCCAAG 
AGCCAGGTGA AGCGGCAGGA GGTCATCAGC GAGCTCCTGG TGACGGAGGC TGCCCATGTG 
CGCATGCTCC GCGTGCTCCA CGACGTCTTC TACCAGCCCA TAGCGGACGG GGGCTTCTAC 
TCCCAGGAGG AGCTCCAGAA CATCTTCCCC AGCCTGGACG AGCTCATCGA GGTGCATTCC 
CTATTCCTTG ATTCCCTGAT GAAGCGGAGG CAGGACAGTG GCTACCTCAT CAAGGAGATC 
GGAGATGTGC TGCTGGCCCG GTTCGATGGT GCCGAGGGCT CGTGGTTCCA GAAAATCTCC 
TCCCGCTTCT GTAGCCGCCA GTCGTTCGCC TTAGAGCAGC TCAAAGCCAA ACAGCGCAAG 
GAGCCCCGGT TCTGTACCTT CGTGCAGGAG GCCGAGAGCC GGCCCCGGTG CCGCCGGCTG 
CAGCTGAAGG ACATGATCCC CACCGAGATG CAGCGTCTGA CCAAGTACCC ACTGCTTCTG 
CAGAGCATCG AGCAGAACAC AGAAGAGCCG GTGGAACGGG AGAAAGTGGA GCGGGCAGCC 
GAGTGCTGCC GGGAAATTCT GCAACACGTC AACCTTGCCG TGCGGGACAT GGAGGACCTG 
CTGCGGCTCA AAGATTATCA GAGGCGTCTG GATTTGACCC ACCTGCGGCA GAGCAACGAC 
CCCATGCTGA GCGAGTTCAA GAACCTAGAC ATCACCAAGA AGAGGTTGAT CTACGAGGGC 
CCACTGACGT GGCGGGTGAC GAAGGACAAG GCTGTGGAGG TCCACGTGCT GCTGCTGGAT 
GACCTGCTGC TGCTGCTTCA GCGCCAGGAC GAGCGGTTCT TGCTTAAGTC GCACAGCCGG 
ACGCTGACGC CCACGCCCGA TGGCAAGACC ATGCTGCGGC CAGTGCTGCG GCTCACCTCC 
GCCATGACCC GCGAGGTGGC CACCGATCAC AAAGCCTTCT ATGTCATTTT TACCTGGGAC 
CAGGAGGCCC AGATATACGA GCTGGTGGCG CAGACGGTGT CTGAGCGGAG GATCTGGTGT 
ACCCTGATCA CCGAGACCGC TGGATGCCTG AAGGTCCCCT CCCGCACCTC CCGACCCAAA 
CACCGGCCCG GCCCCAGCAG CACTCGTGAA CCCCTGCTTA GCAGCTCCGA AAACGGCAAT 
GGCAGCCGAG AGATGCCCCC GGCAGATGGC CGGATGGAGA GACTCTTCAA CTCCCTCCTG 
CCCTTCTGCA GACAAGGCCT CGAGGGCCAG CTCGCCGCCA AGGCCCTTCA GAAAGTGCTG 
ACCCTGAAAC AGCTCCTGTT GCCTTCGGAG GAAGACAGCG GGGCAGGGCC TCCCCTCGAA 
GGGGATGGGG TCCCGGGGGG CAGCCCCTTG AGCCCAACGC AGCCCCAGGA AATTCGGGAG 
GAGCTGCTCA GCCTGGAGGA GACCATTAAA CAGCTAGAGG AGGTGGAGGA GGAATTTTGC 
CGCCTGAGAC TCCTCCTGTC TCAGCTTGGG GAGAACACCG TCCCCCAGTC TGGCTGTACC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024834887.1
CDS	281..3043
Translation	MEFSSPAVPMEAEDVARGAAPGPPRPGLAPVSIIGAEDEDFEIELKTNPEEQNNQFQNLDQVKRRPAHLMALLQHVAMQFEPGPLLCCLHADMLGSLGPKEFKKAFIDFYHSFLDKHSILRVMVPPAVAFELDRTRPDLFPEDLQRQFVQEVVQSQQVAVVRQLEDFRSKKLMGMTPWEQELVQLGAWVARDRASYEARERHLAERQLTHLEEMQHTISNDEEKSAAVVNAISMYMRHLGVRTKSGDKKSGRNFFRKKMIGNRRSDEPTKTKKGLSSFLDAARWNRGEPQDFRHPKVEVDAEKPGLTERKGGQGAPSRDRNLGGPGQDTPGVSPHLLSGDSPDREPGVDALLEVGDPPPQGPASLDAPAPLESTEEAADTESPEPGDEGEPGRSGLELEPEEPPGWRELVPQDILHSLPKSQVKRQEVISELLVTEAAHVRMLRVLHDVFYQPIADGGFYSQEELQNIFPSLDELIEVHSLFLDSLMKRRQDSGYLIKEIGDVLLARFDGAEGSWFQKISSRFCSRQSFALEQLKAKQRKEPRFCTFVQEAESRPRCRRLQLKDMIPTEMQRLTKYPLLLQSIEQNTEEPVEREKVERAAECCREILQHVNLAVRDMEDLLRLKDYQRRLDLTHLRQSNDPMLSEFKNLDITKKRLIYEGPLTWRVTKDKAVEVHVLLLDDLLLLLQRQDERFLLKSHSRTLTPTPDGKTMLRPVLRLTSAMTREVATDHKAFYVIFTWDQEAQIYELVAQTVSERRIWCTLITETAGCLKVPSRTSRPKHRPGPSSTREPLLSSSENGNGSREMPPADGRMERLFNSLLPFCRQGLEGQLAAKALQKVLTLKQLLLPSEEDSGAGPPLEGDGVPGGSPLSPTQPQEIREELLSLEETIKQLEEVEEEFCRLRLLLSQLGENTVPQSGCT

Target ORF information:

RefSeq Version	XM_024979119.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024979119.1

ORF Insert Sequence:

ATGGAATTCT CCAGCCCGGC AGTGCCCATG GAGGCAGAAG ACGTCGCCCG AGGGGCGGCC 
CCGGGGCCCC CCCGACCCGG CCTGGCGCCC GTCAGCATCA TTGGGGCAGA GGACGAGGAT 
TTTGAGATTG AGTTGAAGAC GAACCCTGAG GAACAAAACA ACCAGTTCCA GAACCTGGAT 
CAGGTGAAGC GGCGCCCGGC CCACCTCATG GCCCTCCTGC AGCACGTGGC CATGCAGTTT 
GAGCCGGGAC CCCTGCTCTG CTGCCTGCAT GCGGACATGC TGGGCTCCCT GGGCCCCAAG 
GAGTTCAAGA AAGCCTTCAT CGACTTCTAC CACAGCTTCC TGGATAAGCA CTCGATTCTA 
CGAGTGATGG TCCCTCCTGC CGTGGCCTTT GAACTTGACC GCACGCGGCC TGACCTCTTT 
CCTGAGGATC TCCAGCGGCA GTTCGTGCAG GAGGTGGTGC AGAGCCAGCA GGTAGCCGTG 
GTCCGGCAGC TGGAGGACTT CCGCTCCAAG AAGCTCATGG GCATGACACC CTGGGAGCAG 
GAGCTGGTCC AGCTGGGGGC CTGGGTGGCG CGGGACCGTG CCAGCTACGA GGCCCGGGAG 
CGGCACCTGG CTGAGCGGCA ACTGACCCAC CTGGAGGAGA TGCAGCACAC CATCTCTAAC 
GATGAGGAGA AGAGCGCCGC CGTGGTCAAC GCCATCAGCA TGTACATGCG GCACCTTGGG 
GTGCGGACCA AGAGTGGGGA CAAGAAGTCC GGGAGGAACT TCTTTCGGAA AAAGATGATA 
GGGAACCGGC GGTCAGATGA ACCGACCAAG ACCAAGAAAG GCCTGAGCAG CTTCCTGGAT 
GCCGCCCGCT GGAACCGGGG AGAGCCCCAG GATTTTCGAC ACCCGAAGGT CGAAGTTGAT 
GCTGAGAAGC CAGGCCTTAC AGAACGTAAG GGAGGTCAGG GGGCGCCTTC CCGGGACCGG 
AATCTCGGAG GCCCTGGGCA GGACACCCCC GGAGTTTCTC CGCACCTTCT GTCTGGGGAC 
AGCCCTGACC GGGAGCCAGG TGTTGACGCC CTCCTGGAGG TGGGGGACCC GCCCCCACAG 
GGCCCAGCCA GCCTGGACGC CCCAGCGCCG CTGGAGAGCA CGGAGGAGGC TGCCGACACA 
GAAAGCCCGG AGCCTGGAGA TGAGGGAGAG CCGGGCCGCT CAGGACTGGA GCTGGAACCA 
GAGGAGCCGC CTGGCTGGCG AGAGCTTGTC CCCCAGGACA TTCTGCACAG CCTGCCCAAG 
AGCCAGGTGA AGCGGCAGGA GGTCATCAGC GAGCTCCTGG TGACGGAGGC TGCCCATGTG 
CGCATGCTCC GCGTGCTCCA CGACGTCTTC TACCAGCCCA TAGCGGACGG GGGCTTCTAC 
TCCCAGGAGG AGCTCCAGAA CATCTTCCCC AGCCTGGACG AGCTCATCGA GGTGCATTCC 
CTATTCCTTG ATTCCCTGAT GAAGCGGAGG CAGGACAGTG GCTACCTCAT CAAGGAGATC 
GGAGATGTGC TGCTGGCCCG GTTCGATGGT GCCGAGGGCT CGTGGTTCCA GAAAATCTCC 
TCCCGCTTCT GTAGCCGCCA GTCGTTCGCC TTAGAGCAGC TCAAAGCCAA ACAGCGCAAG 
GAGCCCCGGT TCTGTACCTT CGTGCAGGAG GCCGAGAGCC GGCCCCGGTG CCGCCGGCTG 
CAGCTGAAGG ACATGATCCC CACCGAGATG CAGCGTCTGA CCAAGTACCC ACTGCTTCTG 
CAGAGCATCG AGCAGAACAC AGAAGAGCCG GTGGAACGGG AGAAAGTGGA GCGGGCAGCC 
GAGTGCTGCC GGGAAATTCT GCAACACGTC AACCTTGCCG TGCGGGACAT GGAGGACCTG 
CTGCGGCTCA AAGATTATCA GAGGCGTCTG GATTTGACCC ACCTGCGGCA GAGCAACGAC 
CCCATGCTGA GCGAGTTCAA GAACCTAGAC ATCACCAAGA AGAGGTTGAT CTACGAGGGC 
CCACTGACGT GGCGGGTGAC GAAGGACAAG GCTGTGGAGG TCCACGTGCT GCTGCTGGAT 
GACCTGCTGC TGCTGCTTCA GCGCCAGGAC GAGCGGTTCT TGCTTAAGTC GCACAGCCGG 
ACGCTGACGC CCACGCCCGA TGGCAAGACC ATGCTGCGGC CAGTGCTGCG GCTCACCTCC 
GCCATGACCC GCGAGGTGGC CACCGATCAC AAAGCCTTCT ATGTCATTTT TACCTGGGAC 
CAGGAGGCCC AGATATACGA GCTGGTGGCG CAGACGGTGT CTGAGCGGAG GATCTGGTGT 
ACCCTGATCA CCGAGACCGC TGGATGCCTG AAGGTCCCCT CCCGCACCTC CCGACCCAAA 
CACCGGCCCG GCCCCAGCAG CACTCGTGAA CCCCTGCTTA GCAGCTCCGA AAACGGCAAT 
GGCAGCCGAG AGATGCCCCC GGCAGATGGC CGGATGGAGA GACTCTTCAA CTCCCTCCTG 
CCCTTCTGCA GACAAGGCCT CGAGGGCCAG CTCGCCGCCA AGGCCCTTCA GAAAGTGCTG 
ACCCTGAAAC AGCTCCTGTT GCCTTCGGAG GAAGACAGCG GGGCAGGGCC TCCCCTCGAA 
GGGGATGGGG TCCCGGGGGG CAGCCCCTTG AGCCCAACGC AGCCCCAGGA AATTCGGGAG 
GAGCTGCTCA GCCTGGAGGA GACCATTAAA CAGCTAGAGG AGGTGGAGGA GGAATTTTGC 
CGCCTGAGAC TCCTCCTGTC TCAGCTTGGG GAGAACACCG TCCCCCAGTC TGGCTGTACC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF1 ( XM_024979121.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024834889.1 Bos taurus Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OBa225595
Clone ID Related Accession (Same CDS sequence)	XM_024979121.1 , XM_024979120.1
Accession Version	XM_024979121.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2736bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rho guanine nucleotide exchange factor 1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCAG AAGACGTCGC CCGAGGGGCG GCCCCGGGGC CCCCCCGACC CGGCCTGGCG 
CCCGTCAGCA TCATTGGGGC AGAGGACGAG GATTTTGAGA TTGAGTTGAA GACGAACCCT 
GAGGAACAAA ACAACCAGTT CCAGAACCTG GATCAGGTGA AGCGGCGCCC GGCCCACCTC 
ATGGCCCTCC TGCAGCACGT GGCCATGCAG TTTGAGCCGG GACCCCTGCT CTGCTGCCTG 
CATGCGGACA TGCTGGGCTC CCTGGGCCCC AAGGAGTTCA AGAAAGCCTT CATCGACTTC 
TACCACAGCT TCCTGGATAA GCACTCGATT CTACGAGTGA TGGTCCCTCC TGCCGTGGCC 
TTTGAACTTG ACCGCACGCG GCCTGACCTC TTTCCTGAGG ATCTCCAGCG GCAGTTCGTG 
CAGGAGGTGG TGCAGAGCCA GCAGGTAGCC GTGGTCCGGC AGCTGGAGGA CTTCCGCTCC 
AAGAAGCTCA TGGGCATGAC ACCCTGGGAG CAGGAGCTGG TCCAGCTGGG GGCCTGGGTG 
GCGCGGGACC GTGCCAGCTA CGAGGCCCGG GAGCGGCACC TGGCTGAGCG GCAACTGACC 
CACCTGGAGG AGATGCAGCA CACCATCTCT AACGATGAGG AGAAGAGCGC CGCCGTGGTC 
AACGCCATCA GCATGTACAT GCGGCACCTT GGGGTGCGGA CCAAGAGTGG GGACAAGAAG 
TCCGGGAGGA ACTTCTTTCG GAAAAAGATG ATAGGGAACC GGCGGTCAGA TGAACCGACC 
AAGACCAAGA AAGGCCTGAG CAGCTTCCTG GATGCCGCCC GCTGGAACCG GGGAGAGCCC 
CAGGATTTTC GACACCCGAA GGTCGAAGTT GATGCTGAGA AGCCAGGCCT TACAGAACGT 
AAGGGAGGTC AGGGGGCGCC TTCCCGGGAC CGGAATCTCG GAGGCCCTGG GCAGGACACC 
CCCGGAGTTT CTCCGCACCT TCTGTCTGGG GACAGCCCTG ACCGGGAGCC AGGTGTTGAC 
GCCCTCCTGG AGGTGGGGGA CCCGCCCCCA CAGGGCCCAG CCAGCCTGGA CGCCCCAGCG 
CCGCTGGAGA GCACGGAGGA GGCTGCCGAC ACAGAAAGCC CGGAGCCTGG AGATGAGGGA 
GAGCCGGGCC GCTCAGGACT GGAGCTGGAA CCAGAGGAGC CGCCTGGCTG GCGAGAGCTT 
GTCCCCCAGG ACATTCTGCA CAGCCTGCCC AAGAGCCAGG TGAAGCGGCA GGAGGTCATC 
AGCGAGCTCC TGGTGACGGA GGCTGCCCAT GTGCGCATGC TCCGCGTGCT CCACGACGTC 
TTCTACCAGC CCATAGCGGA CGGGGGCTTC TACTCCCAGG AGGAGCTCCA GAACATCTTC 
CCCAGCCTGG ACGAGCTCAT CGAGGTGCAT TCCCTATTCC TTGATTCCCT GATGAAGCGG 
AGGCAGGACA GTGGCTACCT CATCAAGGAG ATCGGAGATG TGCTGCTGGC CCGGTTCGAT 
GGTGCCGAGG GCTCGTGGTT CCAGAAAATC TCCTCCCGCT TCTGTAGCCG CCAGTCGTTC 
GCCTTAGAGC AGCTCAAAGC CAAACAGCGC AAGGAGCCCC GGTTCTGTAC CTTCGTGCAG 
GAGGCCGAGA GCCGGCCCCG GTGCCGCCGG CTGCAGCTGA AGGACATGAT CCCCACCGAG 
ATGCAGCGTC TGACCAAGTA CCCACTGCTT CTGCAGAGCA TCGAGCAGAA CACAGAAGAG 
CCGGTGGAAC GGGAGAAAGT GGAGCGGGCA GCCGAGTGCT GCCGGGAAAT TCTGCAACAC 
GTCAACCTTG CCGTGCGGGA CATGGAGGAC CTGCTGCGGC TCAAAGATTA TCAGAGGCGT 
CTGGATTTGA CCCACCTGCG GCAGAGCAAC GACCCCATGC TGAGCGAGTT CAAGAACCTA 
GACATCACCA AGAAGAGGTT GATCTACGAG GGCCCACTGA CGTGGCGGGT GACGAAGGAC 
AAGGCTGTGG AGGTCCACGT GCTGCTGCTG GATGACCTGC TGCTGCTGCT TCAGCGCCAG 
GACGAGCGGT TCTTGCTTAA GTCGCACAGC CGGACGCTGA CGCCCACGCC CGATGGCAAG 
ACCATGCTGC GGCCAGTGCT GCGGCTCACC TCCGCCATGA CCCGCGAGGT GGCCACCGAT 
CACAAAGCCT TCTATGTCAT TTTTACCTGG GACCAGGAGG CCCAGATATA CGAGCTGGTG 
GCGCAGACGG TGTCTGAGCG GAGGATCTGG TGTACCCTGA TCACCGAGAC CGCTGGATGC 
CTGAAGGTCC CCTCCCGCAC CTCCCGACCC AAACACCGGC CCGGCCCCAG CAGCACTCGT 
GAACCCCTGC TTAGCAGCTC CGAAAACGGC AATGGCAGCC GAGAGATGCC CCCGGCAGAT 
GGCCGGATGG AGAGACTCTT CAACTCCCTC CTGCCCTTCT GCAGACAAGG CCTCGAGGGC 
CAGCTCGCCG CCAAGGCCCT TCAGAAAGTG CTGACCCTGA AACAGCTCCT GTTGCCTTCG 
GAGGAAGACA GCGGGGCAGG GCCTCCCCTC GAAGGGGATG GGGTCCCGGG GGGCAGCCCC 
TTGAGCCCAA CGCAGCCCCA GGAAATTCGG GAGGAGCTGC TCAGCCTGGA GGAGACCATT 
AAACAGCTAG AGGAGGTGGA GGAGGAATTT TGCCGCCTGA GACTCCTCCT GTCTCAGCTT 
GGGGAGAACA CCGTCCCCCA GTCTGGCTGT ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024834889.1
CDS	150..2885
Translation	MEAEDVARGAAPGPPRPGLAPVSIIGAEDEDFEIELKTNPEEQNNQFQNLDQVKRRPAHLMALLQHVAMQFEPGPLLCCLHADMLGSLGPKEFKKAFIDFYHSFLDKHSILRVMVPPAVAFELDRTRPDLFPEDLQRQFVQEVVQSQQVAVVRQLEDFRSKKLMGMTPWEQELVQLGAWVARDRASYEARERHLAERQLTHLEEMQHTISNDEEKSAAVVNAISMYMRHLGVRTKSGDKKSGRNFFRKKMIGNRRSDEPTKTKKGLSSFLDAARWNRGEPQDFRHPKVEVDAEKPGLTERKGGQGAPSRDRNLGGPGQDTPGVSPHLLSGDSPDREPGVDALLEVGDPPPQGPASLDAPAPLESTEEAADTESPEPGDEGEPGRSGLELEPEEPPGWRELVPQDILHSLPKSQVKRQEVISELLVTEAAHVRMLRVLHDVFYQPIADGGFYSQEELQNIFPSLDELIEVHSLFLDSLMKRRQDSGYLIKEIGDVLLARFDGAEGSWFQKISSRFCSRQSFALEQLKAKQRKEPRFCTFVQEAESRPRCRRLQLKDMIPTEMQRLTKYPLLLQSIEQNTEEPVEREKVERAAECCREILQHVNLAVRDMEDLLRLKDYQRRLDLTHLRQSNDPMLSEFKNLDITKKRLIYEGPLTWRVTKDKAVEVHVLLLDDLLLLLQRQDERFLLKSHSRTLTPTPDGKTMLRPVLRLTSAMTREVATDHKAFYVIFTWDQEAQIYELVAQTVSERRIWCTLITETAGCLKVPSRTSRPKHRPGPSSTREPLLSSSENGNGSREMPPADGRMERLFNSLLPFCRQGLEGQLAAKALQKVLTLKQLLLPSEEDSGAGPPLEGDGVPGGSPLSPTQPQEIREELLSLEETIKQLEEVEEEFCRLRLLLSQLGENTVPQSGCT

Target ORF information:

RefSeq Version	XM_024979121.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024979121.1

ORF Insert Sequence:

ATGGAGGCAG AAGACGTCGC CCGAGGGGCG GCCCCGGGGC CCCCCCGACC CGGCCTGGCG 
CCCGTCAGCA TCATTGGGGC AGAGGACGAG GATTTTGAGA TTGAGTTGAA GACGAACCCT 
GAGGAACAAA ACAACCAGTT CCAGAACCTG GATCAGGTGA AGCGGCGCCC GGCCCACCTC 
ATGGCCCTCC TGCAGCACGT GGCCATGCAG TTTGAGCCGG GACCCCTGCT CTGCTGCCTG 
CATGCGGACA TGCTGGGCTC CCTGGGCCCC AAGGAGTTCA AGAAAGCCTT CATCGACTTC 
TACCACAGCT TCCTGGATAA GCACTCGATT CTACGAGTGA TGGTCCCTCC TGCCGTGGCC 
TTTGAACTTG ACCGCACGCG GCCTGACCTC TTTCCTGAGG ATCTCCAGCG GCAGTTCGTG 
CAGGAGGTGG TGCAGAGCCA GCAGGTAGCC GTGGTCCGGC AGCTGGAGGA CTTCCGCTCC 
AAGAAGCTCA TGGGCATGAC ACCCTGGGAG CAGGAGCTGG TCCAGCTGGG GGCCTGGGTG 
GCGCGGGACC GTGCCAGCTA CGAGGCCCGG GAGCGGCACC TGGCTGAGCG GCAACTGACC 
CACCTGGAGG AGATGCAGCA CACCATCTCT AACGATGAGG AGAAGAGCGC CGCCGTGGTC 
AACGCCATCA GCATGTACAT GCGGCACCTT GGGGTGCGGA CCAAGAGTGG GGACAAGAAG 
TCCGGGAGGA ACTTCTTTCG GAAAAAGATG ATAGGGAACC GGCGGTCAGA TGAACCGACC 
AAGACCAAGA AAGGCCTGAG CAGCTTCCTG GATGCCGCCC GCTGGAACCG GGGAGAGCCC 
CAGGATTTTC GACACCCGAA GGTCGAAGTT GATGCTGAGA AGCCAGGCCT TACAGAACGT 
AAGGGAGGTC AGGGGGCGCC TTCCCGGGAC CGGAATCTCG GAGGCCCTGG GCAGGACACC 
CCCGGAGTTT CTCCGCACCT TCTGTCTGGG GACAGCCCTG ACCGGGAGCC AGGTGTTGAC 
GCCCTCCTGG AGGTGGGGGA CCCGCCCCCA CAGGGCCCAG CCAGCCTGGA CGCCCCAGCG 
CCGCTGGAGA GCACGGAGGA GGCTGCCGAC ACAGAAAGCC CGGAGCCTGG AGATGAGGGA 
GAGCCGGGCC GCTCAGGACT GGAGCTGGAA CCAGAGGAGC CGCCTGGCTG GCGAGAGCTT 
GTCCCCCAGG ACATTCTGCA CAGCCTGCCC AAGAGCCAGG TGAAGCGGCA GGAGGTCATC 
AGCGAGCTCC TGGTGACGGA GGCTGCCCAT GTGCGCATGC TCCGCGTGCT CCACGACGTC 
TTCTACCAGC CCATAGCGGA CGGGGGCTTC TACTCCCAGG AGGAGCTCCA GAACATCTTC 
CCCAGCCTGG ACGAGCTCAT CGAGGTGCAT TCCCTATTCC TTGATTCCCT GATGAAGCGG 
AGGCAGGACA GTGGCTACCT CATCAAGGAG ATCGGAGATG TGCTGCTGGC CCGGTTCGAT 
GGTGCCGAGG GCTCGTGGTT CCAGAAAATC TCCTCCCGCT TCTGTAGCCG CCAGTCGTTC 
GCCTTAGAGC AGCTCAAAGC CAAACAGCGC AAGGAGCCCC GGTTCTGTAC CTTCGTGCAG 
GAGGCCGAGA GCCGGCCCCG GTGCCGCCGG CTGCAGCTGA AGGACATGAT CCCCACCGAG 
ATGCAGCGTC TGACCAAGTA CCCACTGCTT CTGCAGAGCA TCGAGCAGAA CACAGAAGAG 
CCGGTGGAAC GGGAGAAAGT GGAGCGGGCA GCCGAGTGCT GCCGGGAAAT TCTGCAACAC 
GTCAACCTTG CCGTGCGGGA CATGGAGGAC CTGCTGCGGC TCAAAGATTA TCAGAGGCGT 
CTGGATTTGA CCCACCTGCG GCAGAGCAAC GACCCCATGC TGAGCGAGTT CAAGAACCTA 
GACATCACCA AGAAGAGGTT GATCTACGAG GGCCCACTGA CGTGGCGGGT GACGAAGGAC 
AAGGCTGTGG AGGTCCACGT GCTGCTGCTG GATGACCTGC TGCTGCTGCT TCAGCGCCAG 
GACGAGCGGT TCTTGCTTAA GTCGCACAGC CGGACGCTGA CGCCCACGCC CGATGGCAAG 
ACCATGCTGC GGCCAGTGCT GCGGCTCACC TCCGCCATGA CCCGCGAGGT GGCCACCGAT 
CACAAAGCCT TCTATGTCAT TTTTACCTGG GACCAGGAGG CCCAGATATA CGAGCTGGTG 
GCGCAGACGG TGTCTGAGCG GAGGATCTGG TGTACCCTGA TCACCGAGAC CGCTGGATGC 
CTGAAGGTCC CCTCCCGCAC CTCCCGACCC AAACACCGGC CCGGCCCCAG CAGCACTCGT 
GAACCCCTGC TTAGCAGCTC CGAAAACGGC AATGGCAGCC GAGAGATGCC CCCGGCAGAT 
GGCCGGATGG AGAGACTCTT CAACTCCCTC CTGCCCTTCT GCAGACAAGG CCTCGAGGGC 
CAGCTCGCCG CCAAGGCCCT TCAGAAAGTG CTGACCCTGA AACAGCTCCT GTTGCCTTCG 
GAGGAAGACA GCGGGGCAGG GCCTCCCCTC GAAGGGGATG GGGTCCCGGG GGGCAGCCCC 
TTGAGCCCAA CGCAGCCCCA GGAAATTCGG GAGGAGCTGC TCAGCCTGGA GGAGACCATT 
AAACAGCTAG AGGAGGTGGA GGAGGAATTT TGCCGCCTGA GACTCCTCCT GTCTCAGCTT 
GGGGAGAACA CCGTCCCCCA GTCTGGCTGT ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF1 ( XM_024979120.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024834888.1 Bos taurus Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OBa225595
Clone ID Related Accession (Same CDS sequence)	XM_024979121.1 , XM_024979120.1
Accession Version	XM_024979120.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2736bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rho guanine nucleotide exchange factor 1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCAG AAGACGTCGC CCGAGGGGCG GCCCCGGGGC CCCCCCGACC CGGCCTGGCG 
CCCGTCAGCA TCATTGGGGC AGAGGACGAG GATTTTGAGA TTGAGTTGAA GACGAACCCT 
GAGGAACAAA ACAACCAGTT CCAGAACCTG GATCAGGTGA AGCGGCGCCC GGCCCACCTC 
ATGGCCCTCC TGCAGCACGT GGCCATGCAG TTTGAGCCGG GACCCCTGCT CTGCTGCCTG 
CATGCGGACA TGCTGGGCTC CCTGGGCCCC AAGGAGTTCA AGAAAGCCTT CATCGACTTC 
TACCACAGCT TCCTGGATAA GCACTCGATT CTACGAGTGA TGGTCCCTCC TGCCGTGGCC 
TTTGAACTTG ACCGCACGCG GCCTGACCTC TTTCCTGAGG ATCTCCAGCG GCAGTTCGTG 
CAGGAGGTGG TGCAGAGCCA GCAGGTAGCC GTGGTCCGGC AGCTGGAGGA CTTCCGCTCC 
AAGAAGCTCA TGGGCATGAC ACCCTGGGAG CAGGAGCTGG TCCAGCTGGG GGCCTGGGTG 
GCGCGGGACC GTGCCAGCTA CGAGGCCCGG GAGCGGCACC TGGCTGAGCG GCAACTGACC 
CACCTGGAGG AGATGCAGCA CACCATCTCT AACGATGAGG AGAAGAGCGC CGCCGTGGTC 
AACGCCATCA GCATGTACAT GCGGCACCTT GGGGTGCGGA CCAAGAGTGG GGACAAGAAG 
TCCGGGAGGA ACTTCTTTCG GAAAAAGATG ATAGGGAACC GGCGGTCAGA TGAACCGACC 
AAGACCAAGA AAGGCCTGAG CAGCTTCCTG GATGCCGCCC GCTGGAACCG GGGAGAGCCC 
CAGGATTTTC GACACCCGAA GGTCGAAGTT GATGCTGAGA AGCCAGGCCT TACAGAACGT 
AAGGGAGGTC AGGGGGCGCC TTCCCGGGAC CGGAATCTCG GAGGCCCTGG GCAGGACACC 
CCCGGAGTTT CTCCGCACCT TCTGTCTGGG GACAGCCCTG ACCGGGAGCC AGGTGTTGAC 
GCCCTCCTGG AGGTGGGGGA CCCGCCCCCA CAGGGCCCAG CCAGCCTGGA CGCCCCAGCG 
CCGCTGGAGA GCACGGAGGA GGCTGCCGAC ACAGAAAGCC CGGAGCCTGG AGATGAGGGA 
GAGCCGGGCC GCTCAGGACT GGAGCTGGAA CCAGAGGAGC CGCCTGGCTG GCGAGAGCTT 
GTCCCCCAGG ACATTCTGCA CAGCCTGCCC AAGAGCCAGG TGAAGCGGCA GGAGGTCATC 
AGCGAGCTCC TGGTGACGGA GGCTGCCCAT GTGCGCATGC TCCGCGTGCT CCACGACGTC 
TTCTACCAGC CCATAGCGGA CGGGGGCTTC TACTCCCAGG AGGAGCTCCA GAACATCTTC 
CCCAGCCTGG ACGAGCTCAT CGAGGTGCAT TCCCTATTCC TTGATTCCCT GATGAAGCGG 
AGGCAGGACA GTGGCTACCT CATCAAGGAG ATCGGAGATG TGCTGCTGGC CCGGTTCGAT 
GGTGCCGAGG GCTCGTGGTT CCAGAAAATC TCCTCCCGCT TCTGTAGCCG CCAGTCGTTC 
GCCTTAGAGC AGCTCAAAGC CAAACAGCGC AAGGAGCCCC GGTTCTGTAC CTTCGTGCAG 
GAGGCCGAGA GCCGGCCCCG GTGCCGCCGG CTGCAGCTGA AGGACATGAT CCCCACCGAG 
ATGCAGCGTC TGACCAAGTA CCCACTGCTT CTGCAGAGCA TCGAGCAGAA CACAGAAGAG 
CCGGTGGAAC GGGAGAAAGT GGAGCGGGCA GCCGAGTGCT GCCGGGAAAT TCTGCAACAC 
GTCAACCTTG CCGTGCGGGA CATGGAGGAC CTGCTGCGGC TCAAAGATTA TCAGAGGCGT 
CTGGATTTGA CCCACCTGCG GCAGAGCAAC GACCCCATGC TGAGCGAGTT CAAGAACCTA 
GACATCACCA AGAAGAGGTT GATCTACGAG GGCCCACTGA CGTGGCGGGT GACGAAGGAC 
AAGGCTGTGG AGGTCCACGT GCTGCTGCTG GATGACCTGC TGCTGCTGCT TCAGCGCCAG 
GACGAGCGGT TCTTGCTTAA GTCGCACAGC CGGACGCTGA CGCCCACGCC CGATGGCAAG 
ACCATGCTGC GGCCAGTGCT GCGGCTCACC TCCGCCATGA CCCGCGAGGT GGCCACCGAT 
CACAAAGCCT TCTATGTCAT TTTTACCTGG GACCAGGAGG CCCAGATATA CGAGCTGGTG 
GCGCAGACGG TGTCTGAGCG GAGGATCTGG TGTACCCTGA TCACCGAGAC CGCTGGATGC 
CTGAAGGTCC CCTCCCGCAC CTCCCGACCC AAACACCGGC CCGGCCCCAG CAGCACTCGT 
GAACCCCTGC TTAGCAGCTC CGAAAACGGC AATGGCAGCC GAGAGATGCC CCCGGCAGAT 
GGCCGGATGG AGAGACTCTT CAACTCCCTC CTGCCCTTCT GCAGACAAGG CCTCGAGGGC 
CAGCTCGCCG CCAAGGCCCT TCAGAAAGTG CTGACCCTGA AACAGCTCCT GTTGCCTTCG 
GAGGAAGACA GCGGGGCAGG GCCTCCCCTC GAAGGGGATG GGGTCCCGGG GGGCAGCCCC 
TTGAGCCCAA CGCAGCCCCA GGAAATTCGG GAGGAGCTGC TCAGCCTGGA GGAGACCATT 
AAACAGCTAG AGGAGGTGGA GGAGGAATTT TGCCGCCTGA GACTCCTCCT GTCTCAGCTT 
GGGGAGAACA CCGTCCCCCA GTCTGGCTGT ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024834888.1
CDS	489..3224
Translation	MEAEDVARGAAPGPPRPGLAPVSIIGAEDEDFEIELKTNPEEQNNQFQNLDQVKRRPAHLMALLQHVAMQFEPGPLLCCLHADMLGSLGPKEFKKAFIDFYHSFLDKHSILRVMVPPAVAFELDRTRPDLFPEDLQRQFVQEVVQSQQVAVVRQLEDFRSKKLMGMTPWEQELVQLGAWVARDRASYEARERHLAERQLTHLEEMQHTISNDEEKSAAVVNAISMYMRHLGVRTKSGDKKSGRNFFRKKMIGNRRSDEPTKTKKGLSSFLDAARWNRGEPQDFRHPKVEVDAEKPGLTERKGGQGAPSRDRNLGGPGQDTPGVSPHLLSGDSPDREPGVDALLEVGDPPPQGPASLDAPAPLESTEEAADTESPEPGDEGEPGRSGLELEPEEPPGWRELVPQDILHSLPKSQVKRQEVISELLVTEAAHVRMLRVLHDVFYQPIADGGFYSQEELQNIFPSLDELIEVHSLFLDSLMKRRQDSGYLIKEIGDVLLARFDGAEGSWFQKISSRFCSRQSFALEQLKAKQRKEPRFCTFVQEAESRPRCRRLQLKDMIPTEMQRLTKYPLLLQSIEQNTEEPVEREKVERAAECCREILQHVNLAVRDMEDLLRLKDYQRRLDLTHLRQSNDPMLSEFKNLDITKKRLIYEGPLTWRVTKDKAVEVHVLLLDDLLLLLQRQDERFLLKSHSRTLTPTPDGKTMLRPVLRLTSAMTREVATDHKAFYVIFTWDQEAQIYELVAQTVSERRIWCTLITETAGCLKVPSRTSRPKHRPGPSSTREPLLSSSENGNGSREMPPADGRMERLFNSLLPFCRQGLEGQLAAKALQKVLTLKQLLLPSEEDSGAGPPLEGDGVPGGSPLSPTQPQEIREELLSLEETIKQLEEVEEEFCRLRLLLSQLGENTVPQSGCT

Target ORF information:

RefSeq Version	XM_024979120.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024979120.1

ORF Insert Sequence:

ATGGAGGCAG AAGACGTCGC CCGAGGGGCG GCCCCGGGGC CCCCCCGACC CGGCCTGGCG 
CCCGTCAGCA TCATTGGGGC AGAGGACGAG GATTTTGAGA TTGAGTTGAA GACGAACCCT 
GAGGAACAAA ACAACCAGTT CCAGAACCTG GATCAGGTGA AGCGGCGCCC GGCCCACCTC 
ATGGCCCTCC TGCAGCACGT GGCCATGCAG TTTGAGCCGG GACCCCTGCT CTGCTGCCTG 
CATGCGGACA TGCTGGGCTC CCTGGGCCCC AAGGAGTTCA AGAAAGCCTT CATCGACTTC 
TACCACAGCT TCCTGGATAA GCACTCGATT CTACGAGTGA TGGTCCCTCC TGCCGTGGCC 
TTTGAACTTG ACCGCACGCG GCCTGACCTC TTTCCTGAGG ATCTCCAGCG GCAGTTCGTG 
CAGGAGGTGG TGCAGAGCCA GCAGGTAGCC GTGGTCCGGC AGCTGGAGGA CTTCCGCTCC 
AAGAAGCTCA TGGGCATGAC ACCCTGGGAG CAGGAGCTGG TCCAGCTGGG GGCCTGGGTG 
GCGCGGGACC GTGCCAGCTA CGAGGCCCGG GAGCGGCACC TGGCTGAGCG GCAACTGACC 
CACCTGGAGG AGATGCAGCA CACCATCTCT AACGATGAGG AGAAGAGCGC CGCCGTGGTC 
AACGCCATCA GCATGTACAT GCGGCACCTT GGGGTGCGGA CCAAGAGTGG GGACAAGAAG 
TCCGGGAGGA ACTTCTTTCG GAAAAAGATG ATAGGGAACC GGCGGTCAGA TGAACCGACC 
AAGACCAAGA AAGGCCTGAG CAGCTTCCTG GATGCCGCCC GCTGGAACCG GGGAGAGCCC 
CAGGATTTTC GACACCCGAA GGTCGAAGTT GATGCTGAGA AGCCAGGCCT TACAGAACGT 
AAGGGAGGTC AGGGGGCGCC TTCCCGGGAC CGGAATCTCG GAGGCCCTGG GCAGGACACC 
CCCGGAGTTT CTCCGCACCT TCTGTCTGGG GACAGCCCTG ACCGGGAGCC AGGTGTTGAC 
GCCCTCCTGG AGGTGGGGGA CCCGCCCCCA CAGGGCCCAG CCAGCCTGGA CGCCCCAGCG 
CCGCTGGAGA GCACGGAGGA GGCTGCCGAC ACAGAAAGCC CGGAGCCTGG AGATGAGGGA 
GAGCCGGGCC GCTCAGGACT GGAGCTGGAA CCAGAGGAGC CGCCTGGCTG GCGAGAGCTT 
GTCCCCCAGG ACATTCTGCA CAGCCTGCCC AAGAGCCAGG TGAAGCGGCA GGAGGTCATC 
AGCGAGCTCC TGGTGACGGA GGCTGCCCAT GTGCGCATGC TCCGCGTGCT CCACGACGTC 
TTCTACCAGC CCATAGCGGA CGGGGGCTTC TACTCCCAGG AGGAGCTCCA GAACATCTTC 
CCCAGCCTGG ACGAGCTCAT CGAGGTGCAT TCCCTATTCC TTGATTCCCT GATGAAGCGG 
AGGCAGGACA GTGGCTACCT CATCAAGGAG ATCGGAGATG TGCTGCTGGC CCGGTTCGAT 
GGTGCCGAGG GCTCGTGGTT CCAGAAAATC TCCTCCCGCT TCTGTAGCCG CCAGTCGTTC 
GCCTTAGAGC AGCTCAAAGC CAAACAGCGC AAGGAGCCCC GGTTCTGTAC CTTCGTGCAG 
GAGGCCGAGA GCCGGCCCCG GTGCCGCCGG CTGCAGCTGA AGGACATGAT CCCCACCGAG 
ATGCAGCGTC TGACCAAGTA CCCACTGCTT CTGCAGAGCA TCGAGCAGAA CACAGAAGAG 
CCGGTGGAAC GGGAGAAAGT GGAGCGGGCA GCCGAGTGCT GCCGGGAAAT TCTGCAACAC 
GTCAACCTTG CCGTGCGGGA CATGGAGGAC CTGCTGCGGC TCAAAGATTA TCAGAGGCGT 
CTGGATTTGA CCCACCTGCG GCAGAGCAAC GACCCCATGC TGAGCGAGTT CAAGAACCTA 
GACATCACCA AGAAGAGGTT GATCTACGAG GGCCCACTGA CGTGGCGGGT GACGAAGGAC 
AAGGCTGTGG AGGTCCACGT GCTGCTGCTG GATGACCTGC TGCTGCTGCT TCAGCGCCAG 
GACGAGCGGT TCTTGCTTAA GTCGCACAGC CGGACGCTGA CGCCCACGCC CGATGGCAAG 
ACCATGCTGC GGCCAGTGCT GCGGCTCACC TCCGCCATGA CCCGCGAGGT GGCCACCGAT 
CACAAAGCCT TCTATGTCAT TTTTACCTGG GACCAGGAGG CCCAGATATA CGAGCTGGTG 
GCGCAGACGG TGTCTGAGCG GAGGATCTGG TGTACCCTGA TCACCGAGAC CGCTGGATGC 
CTGAAGGTCC CCTCCCGCAC CTCCCGACCC AAACACCGGC CCGGCCCCAG CAGCACTCGT 
GAACCCCTGC TTAGCAGCTC CGAAAACGGC AATGGCAGCC GAGAGATGCC CCCGGCAGAT 
GGCCGGATGG AGAGACTCTT CAACTCCCTC CTGCCCTTCT GCAGACAAGG CCTCGAGGGC 
CAGCTCGCCG CCAAGGCCCT TCAGAAAGTG CTGACCCTGA AACAGCTCCT GTTGCCTTCG 
GAGGAAGACA GCGGGGCAGG GCCTCCCCTC GAAGGGGATG GGGTCCCGGG GGGCAGCCCC 
TTGAGCCCAA CGCAGCCCCA GGAAATTCGG GAGGAGCTGC TCAGCCTGGA GGAGACCATT 
AAACAGCTAG AGGAGGTGGA GGAGGAATTT TGCCGCCTGA GACTCCTCCT GTCTCAGCTT 
GGGGAGAACA CCGTCCCCCA GTCTGGCTGT ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF1 ( XM_010815120.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010813422.2 Bos taurus Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa225589
Clone ID Related Accession (Same CDS sequence)	XM_010815120.3
Accession Version	XM_010815120.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3276bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rho guanine nucleotide exchange factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010815120.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGGCA GATCTGAGAA AAGTGGTGCC CGCGCCCTGC GGAGGGACAG ACTGACAAAG 
CCGGGAGAAG CTGGGCGGGG TCCGGGTGGG GCCGATGCCC GCAGGTCCAG GGCGGAGCGG 
GGAAAGGAGG GCGGGGCCGG CGGCGAGAAG GGGAAGGAAG CGGAGGTGCC GGGCGGGCGC 
GGGGCGCGGG CAGGAAGCAA GGAGGGGCGG CGGGGCTGGG GCCTCCGGAA AAACGCCCCA 
ACTTCCTGCC CCGCCAGAGC CAGGAAGCGG GAGCCGGGAC ACCGGGCCCG GGATCGCCGA 
GCCCGACCTC GGGCGCCCCG CCGGTCACCA CCCCGCGCGG ACACCAGCCC GGCAGTGCCC 
ATGGAGGCAG AAGACGTCGC CCGAGGGGCG GCCCCGGGGC CCCCCCGACC CGGCCTGGCG 
CCCGTCAGCA TCATTGGGGC AGAGGACGAG GATTTTGAGA TTGAGTTGAA GACGAACCCT 
GAGGAACAAA ACAACCAGTT CCAGAACCTG GATCAGGTGA AGCGGCGCCC GGCCCACCTC 
ATGGCCCTCC TGCAGCACGT GGCCATGCAG TTTGAGCCGG GACCCCTGCT CTGCTGCCTG 
CATGCGGACA TGCTGGGCTC CCTGGGCCCC AAGGAGTTCA AGAAAGCCTT CATCGACTTC 
TACCACAGCT TCCTGGATAA GCACTCGATT CTACGAGTGA TGGTCCCTCC TGCCGTGGCC 
TTTGAACTTG ACCGCACGCG GCCTGACCTC TTTCCTGAGG ATCTCCAGCG GCAGTTCGTG 
CAGGAGGTGG TGCAGAGCCA GCAGGTAGCC GTGGTCCGGC AGCTGGAGGA CTTCCGCTCC 
AAGAAGCTCA TGGGCATGAC ACCCTGGGAG CAGGAGCTGG TCCAGCTGGG GGCCTGGGTG 
GCGCGGGACC GTGCCAGCTA CGAGGCCCGG GAGCGGCACC TGGCTGAGCG GCAACTGACC 
CACCTGGAGG AGATGCAGCA CACCATCTCT AACGATGAGG AGAAGAGCGC CGCCGTGGTC 
AACGCCATCA GCATGTACAT GCGGCACCTT GGGGTGCGGA CCAAGAGTGG GGACAAGAAG 
TCCGGGAGGA ACTTCTTTCG GAAAAAGATG ATAGGGAACC GGCGGTCAGA TGAACCGACC 
AAGACCAAGA AAGGCCTGAG CAGCTTCCTG GATGCCGCCC GCTGGAACCG GGGAGAGCCC 
CAGGATTTTC GACACCCGAA GGTCGAAGTT GATGCTGAGA AGCCAGGCCT TACAGAACGT 
AAGGGAGGTC AGGGGGCGCC TTCCCGGGAC CGGAATCTCG GAGGCCCTGG GCAGGACACC 
CCCGGAGTTT CTCCGCACCT TCTGTCTGGG GACAGCCCTG ACCGGGAGCC AGGTGTTGAC 
GCCCTCCTGG AGGTGGGGGA CCCGCCCCCA CAGGGCCCAG CCAGCCTGGA CGCCCCAGCG 
CCGCTGGAGA GCACGGAGGA GGCTGCCGAC ACAGAAAGAA AGTGGAAGAG GCTGTCAGGG 
CGTCTGGGGC GCTCAGAGAG CCTGCGGGTG AGTGACCGCC GCCGGCCTTC CCGGGGTAGC 
CTCGGGGCTA AGGGCCGGGG TGGGGGCCGC TCCCGGAGCG ACGTGGACAT GGACCCCAGC 
TCCGCCACGG CCGTGCTTGG CCCTACCCGA CGAGCCACCC CGGAGCCTGG AGATGAGGGA 
GAGCCGGGCC GCTCAGGACT GGAGCTGGAA CCAGAGGAGC CGCCTGGCTG GCGAGAGCTT 
GTCCCCCAGG ACATTCTGCA CAGCCTGCCC AAGAGCCAGG TGAAGCGGCA GGAGGTCATC 
AGCGAGCTCC TGGTGACGGA GGCTGCCCAT GTGCGCATGC TCCGCGTGCT CCACGACGTC 
TTCTACCAGC CCATAGCGGA CGGGGGCTTC TACTCCCAGG AGGAGCTCCA GAACATCTTC 
CCCAGCCTGG ACGAGCTCAT CGAGGTGCAT TCCCTATTCC TTGATTCCCT GATGAAGCGG 
AGGCAGGACA GTGGCTACCT CATCAAGGAG ATCGGAGATG TGCTGCTGGC CCGGTTCGAT 
GGTGCCGAGG GCTCGTGGTT CCAGAAAATC TCCTCCCGCT TCTGTAGCCG CCAGTCGTTC 
GCCTTAGAGC AGCTCAAAGC CAAACAGCGC AAGGAGCCCC GGTTCTGTAC CTTCGTGCAG 
GAGGCCGAGA GCCGGCCCCG GTGCCGCCGG CTGCAGCTGA AGGACATGAT CCCCACCGAG 
ATGCAGCGTC TGACCAAGTA CCCACTGCTT CTGCAGAGCA TCGAGCAGAA CACAGAAGAG 
CCGGTGGAAC GGGAGAAAGT GGAGCGGGCA GCCGAGTGCT GCCGGGAAAT TCTGCAACAC 
GTCAACCTTG CCGTGCGGGA CATGGAGGAC CTGCTGCGGC TCAAAGATTA TCAGAGGCGT 
CTGGATTTGA CCCACCTGCG GCAGAGCAAC GACCCCATGC TGAGCGAGTT CAAGAACCTA 
GACATCACCA AGAAGAGGTT GATCTACGAG GGCCCACTGA CGTGGCGGGT GACGAAGGAC 
AAGGCTGTGG AGGTCCACGT GCTGCTGCTG GATGACCTGC TGCTGCTGCT TCAGCGCCAG 
GACGAGCGGT TCTTGCTTAA GTCGCACAGC CGGACGCTGA CGCCCACGCC CGATGGCAAG 
ACCATGCTGC GGCCAGTGCT GCGGCTCACC TCCGCCATGA CCCGCGAGGT GGCCACCGAT 
CACAAAGCCT TCTATGTCAT TTTTACCTGG GACCAGGAGG CCCAGATATA CGAGCTGGTG 
GCGCAGACGG TGTCTGAGCG GAGGATCTGG TGTACCCTGA TCACCGAGAC CGCTGGATGC 
CTGAAGGTCC CCTCCCGCAC CTCCCGACCC AAACACCGGC CCGGCCCCAG CAGCACTCGT 
GAACCCCTGC TTAGCAGCTC CGAAAACGGC AATGGCAGCC GAGAGATGCC CCCGGCAGAT 
GGCCGGATGG AGAGACTCTT CAACTCCCTC CTGCCCTTCT GCAGACAAGG CCTCGAGGGC 
CAGCTCGCCG CCAAGGCCCT TCAGAAAGTG CTGACCCTGA AACAGCTCCT GTTGCCTTCG 
GAGGAAGACA GCGGGGCAGG GCCTCCCCTC GAAGGGGATG GGGTCCCGGG GGGCAGCCCC 
TTGAGCCCAA CGCAGCCCCA GGAAATTCGG GAGGAGCTGC TCAGCCTGGA GGAGACCATT 
AAACAGCTAG AGGAGGTGGA GGAGGAATTT TGCCGCCTGA GACTCCTCCT GTCTCAGCTT 
GGGGAGAACA CCGTCCCCCA GTCTGGCTGT ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010813422.2
CDS	16..3291
Translation	MSGRSEKSGARALRRDRLTKPGEAGRGPGGADARRSRAERGKEGGAGGEKGKEAEVPGGRGARAGSKEGRRGWGLRKNAPTSCPARARKREPGHRARDRRARPRAPRRSPPRADTSPAVPMEAEDVARGAAPGPPRPGLAPVSIIGAEDEDFEIELKTNPEEQNNQFQNLDQVKRRPAHLMALLQHVAMQFEPGPLLCCLHADMLGSLGPKEFKKAFIDFYHSFLDKHSILRVMVPPAVAFELDRTRPDLFPEDLQRQFVQEVVQSQQVAVVRQLEDFRSKKLMGMTPWEQELVQLGAWVARDRASYEARERHLAERQLTHLEEMQHTISNDEEKSAAVVNAISMYMRHLGVRTKSGDKKSGRNFFRKKMIGNRRSDEPTKTKKGLSSFLDAARWNRGEPQDFRHPKVEVDAEKPGLTERKGGQGAPSRDRNLGGPGQDTPGVSPHLLSGDSPDREPGVDALLEVGDPPPQGPASLDAPAPLESTEEAADTERKWKRLSGRLGRSESLRVSDRRRPSRGSLGAKGRGGGRSRSDVDMDPSSATAVLGPTRRATPEPGDEGEPGRSGLELEPEEPPGWRELVPQDILHSLPKSQVKRQEVISELLVTEAAHVRMLRVLHDVFYQPIADGGFYSQEELQNIFPSLDELIEVHSLFLDSLMKRRQDSGYLIKEIGDVLLARFDGAEGSWFQKISSRFCSRQSFALEQLKAKQRKEPRFCTFVQEAESRPRCRRLQLKDMIPTEMQRLTKYPLLLQSIEQNTEEPVEREKVERAAECCREILQHVNLAVRDMEDLLRLKDYQRRLDLTHLRQSNDPMLSEFKNLDITKKRLIYEGPLTWRVTKDKAVEVHVLLLDDLLLLLQRQDERFLLKSHSRTLTPTPDGKTMLRPVLRLTSAMTREVATDHKAFYVIFTWDQEAQIYELVAQTVSERRIWCTLITETAGCLKVPSRTSRPKHRPGPSSTREPLLSSSENGNGSREMPPADGRMERLFNSLLPFCRQGLEGQLAAKALQKVLTLKQLLLPSEEDSGAGPPLEGDGVPGGSPLSPTQPQEIREELLSLEETIKQLEEVEEEFCRLRLLLSQLGENTVPQSGCT

Target ORF information:

RefSeq Version	XM_010815120.3
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010815120.3

ORF Insert Sequence:

ATGTCAGGCA GATCTGAGAA AAGTGGTGCC CGCGCCCTGC GGAGGGACAG ACTGACAAAG 
CCGGGAGAAG CTGGGCGGGG TCCGGGTGGG GCCGATGCCC GCAGGTCCAG GGCGGAGCGG 
GGAAAGGAGG GCGGGGCCGG CGGCGAGAAG GGGAAGGAAG CGGAGGTGCC GGGCGGGCGC 
GGGGCGCGGG CAGGAAGCAA GGAGGGGCGG CGGGGCTGGG GCCTCCGGAA AAACGCCCCA 
ACTTCCTGCC CCGCCAGAGC CAGGAAGCGG GAGCCGGGAC ACCGGGCCCG GGATCGCCGA 
GCCCGACCTC GGGCGCCCCG CCGGTCACCA CCCCGCGCGG ACACCAGCCC GGCAGTGCCC 
ATGGAGGCAG AAGACGTCGC CCGAGGGGCG GCCCCGGGGC CCCCCCGACC CGGCCTGGCG 
CCCGTCAGCA TCATTGGGGC AGAGGACGAG GATTTTGAGA TTGAGTTGAA GACGAACCCT 
GAGGAACAAA ACAACCAGTT CCAGAACCTG GATCAGGTGA AGCGGCGCCC GGCCCACCTC 
ATGGCCCTCC TGCAGCACGT GGCCATGCAG TTTGAGCCGG GACCCCTGCT CTGCTGCCTG 
CATGCGGACA TGCTGGGCTC CCTGGGCCCC AAGGAGTTCA AGAAAGCCTT CATCGACTTC 
TACCACAGCT TCCTGGATAA GCACTCGATT CTACGAGTGA TGGTCCCTCC TGCCGTGGCC 
TTTGAACTTG ACCGCACGCG GCCTGACCTC TTTCCTGAGG ATCTCCAGCG GCAGTTCGTG 
CAGGAGGTGG TGCAGAGCCA GCAGGTAGCC GTGGTCCGGC AGCTGGAGGA CTTCCGCTCC 
AAGAAGCTCA TGGGCATGAC ACCCTGGGAG CAGGAGCTGG TCCAGCTGGG GGCCTGGGTG 
GCGCGGGACC GTGCCAGCTA CGAGGCCCGG GAGCGGCACC TGGCTGAGCG GCAACTGACC 
CACCTGGAGG AGATGCAGCA CACCATCTCT AACGATGAGG AGAAGAGCGC CGCCGTGGTC 
AACGCCATCA GCATGTACAT GCGGCACCTT GGGGTGCGGA CCAAGAGTGG GGACAAGAAG 
TCCGGGAGGA ACTTCTTTCG GAAAAAGATG ATAGGGAACC GGCGGTCAGA TGAACCGACC 
AAGACCAAGA AAGGCCTGAG CAGCTTCCTG GATGCCGCCC GCTGGAACCG GGGAGAGCCC 
CAGGATTTTC GACACCCGAA GGTCGAAGTT GATGCTGAGA AGCCAGGCCT TACAGAACGT 
AAGGGAGGTC AGGGGGCGCC TTCCCGGGAC CGGAATCTCG GAGGCCCTGG GCAGGACACC 
CCCGGAGTTT CTCCGCACCT TCTGTCTGGG GACAGCCCTG ACCGGGAGCC AGGTGTTGAC 
GCCCTCCTGG AGGTGGGGGA CCCGCCCCCA CAGGGCCCAG CCAGCCTGGA CGCCCCAGCG 
CCGCTGGAGA GCACGGAGGA GGCTGCCGAC ACAGAAAGAA AGTGGAAGAG GCTGTCAGGG 
CGTCTGGGGC GCTCAGAGAG CCTGCGGGTG AGTGACCGCC GCCGGCCTTC CCGGGGTAGC 
CTCGGGGCTA AGGGCCGGGG TGGGGGCCGC TCCCGGAGCG ACGTGGACAT GGACCCCAGC 
TCCGCCACGG CCGTGCTTGG CCCTACCCGA CGAGCCACCC CGGAGCCTGG AGATGAGGGA 
GAGCCGGGCC GCTCAGGACT GGAGCTGGAA CCAGAGGAGC CGCCTGGCTG GCGAGAGCTT 
GTCCCCCAGG ACATTCTGCA CAGCCTGCCC AAGAGCCAGG TGAAGCGGCA GGAGGTCATC 
AGCGAGCTCC TGGTGACGGA GGCTGCCCAT GTGCGCATGC TCCGCGTGCT CCACGACGTC 
TTCTACCAGC CCATAGCGGA CGGGGGCTTC TACTCCCAGG AGGAGCTCCA GAACATCTTC 
CCCAGCCTGG ACGAGCTCAT CGAGGTGCAT TCCCTATTCC TTGATTCCCT GATGAAGCGG 
AGGCAGGACA GTGGCTACCT CATCAAGGAG ATCGGAGATG TGCTGCTGGC CCGGTTCGAT 
GGTGCCGAGG GCTCGTGGTT CCAGAAAATC TCCTCCCGCT TCTGTAGCCG CCAGTCGTTC 
GCCTTAGAGC AGCTCAAAGC CAAACAGCGC AAGGAGCCCC GGTTCTGTAC CTTCGTGCAG 
GAGGCCGAGA GCCGGCCCCG GTGCCGCCGG CTGCAGCTGA AGGACATGAT CCCCACCGAG 
ATGCAGCGTC TGACCAAGTA CCCACTGCTT CTGCAGAGCA TCGAGCAGAA CACAGAAGAG 
CCGGTGGAAC GGGAGAAAGT GGAGCGGGCA GCCGAGTGCT GCCGGGAAAT TCTGCAACAC 
GTCAACCTTG CCGTGCGGGA CATGGAGGAC CTGCTGCGGC TCAAAGATTA TCAGAGGCGT 
CTGGATTTGA CCCACCTGCG GCAGAGCAAC GACCCCATGC TGAGCGAGTT CAAGAACCTA 
GACATCACCA AGAAGAGGTT GATCTACGAG GGCCCACTGA CGTGGCGGGT GACGAAGGAC 
AAGGCTGTGG AGGTCCACGT GCTGCTGCTG GATGACCTGC TGCTGCTGCT TCAGCGCCAG 
GACGAGCGGT TCTTGCTTAA GTCGCACAGC CGGACGCTGA CGCCCACGCC CGATGGCAAG 
ACCATGCTGC GGCCAGTGCT GCGGCTCACC TCCGCCATGA CCCGCGAGGT GGCCACCGAT 
CACAAAGCCT TCTATGTCAT TTTTACCTGG GACCAGGAGG CCCAGATATA CGAGCTGGTG 
GCGCAGACGG TGTCTGAGCG GAGGATCTGG TGTACCCTGA TCACCGAGAC CGCTGGATGC 
CTGAAGGTCC CCTCCCGCAC CTCCCGACCC AAACACCGGC CCGGCCCCAG CAGCACTCGT 
GAACCCCTGC TTAGCAGCTC CGAAAACGGC AATGGCAGCC GAGAGATGCC CCCGGCAGAT 
GGCCGGATGG AGAGACTCTT CAACTCCCTC CTGCCCTTCT GCAGACAAGG CCTCGAGGGC 
CAGCTCGCCG CCAAGGCCCT TCAGAAAGTG CTGACCCTGA AACAGCTCCT GTTGCCTTCG 
GAGGAAGACA GCGGGGCAGG GCCTCCCCTC GAAGGGGATG GGGTCCCGGG GGGCAGCCCC 
TTGAGCCCAA CGCAGCCCCA GGAAATTCGG GAGGAGCTGC TCAGCCTGGA GGAGACCATT 
AAACAGCTAG AGGAGGTGGA GGAGGAATTT TGCCGCCTGA GACTCCTCCT GTCTCAGCTT 
GGGGAGAACA CCGTCCCCCA GTCTGGCTGT ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF1 ( XM_024979112.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024834880.1 Bos taurus Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X2, mRNA.

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CloneID	OBa225590
Clone ID Related Accession (Same CDS sequence)	XM_024979112.1
Accession Version	XM_024979112.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3264bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rho guanine nucleotide exchange factor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGGCA GATCTGAGAA AAGTGGTGCC CGCGCCCTGC GGAGGGACAG ACTGACAAAG 
CCGGGAGAAG CTGGGCGGGG TCCGGGTGGG GCCGATGCCC GCAGGTCCAG GGCGGAGCGG 
GGAAAGGAGG GCGGGGCCGG CGGCGAGAAG GGGAAGGAAG CGGAGGTGCC GGGCGGGCGC 
GGGGCGCGGG CAGGAAGCAA GGAGGGGCGG CGGGGCTGGG GCCTCCGGAA AAACGCCCCA 
ACTTCCTGCC CCGCCAGAGC CAGGAAGCGG GAGCCGGGAC ACCGGGCCCG GGATCGCCGA 
GCCCGACCTC GGGCGCCCCG CCGGTCACCA CCCCGCGCGG ACACCAGCCC GGCAGTGCCC 
ATGGAGGCAG AAGACGTCGC CCGAGGGGCG GCCCCGGGGC CCCCCCGACC CGGCCTGGCG 
CCCGTCAGCA TCATTGGGGC AGAGGACGAG GATTTTGAGA TTGAGTTGAA GACGAACCCT 
GAGGAACAAA ACAACCAGTT CCAGAACCTG GATCAGGTGA AGCGGCGCCC GGCCCACCTC 
ATGGCCCTCC TGCAGCACGT GGCCATGCAG TTTGAGCCGG GACCCCTGCT CTGCTGCCTG 
CATGCGGACA TGCTGGGCTC CCTGGGCCCC AAGGAGTTCA AGAAAGCCTT CATCGACTTC 
TACCACAGCT TCCTGGATAA GCACTCGATT CTACGAGTGA TGGTCCCTCC TGCCGTGGCC 
TTTGAACTTG ACCGCACGCG GCCTGACCTC TTTCCTGAGG ATCTCCAGCG GCAGTTCGTG 
CAGGAGGTGG TGCAGAGCCA GCAGGTAGCC GTGGTCCGGC AGCTGGAGGA CTTCCGCTCC 
AAGAAGCTCA TGGGCATGAC ACCCTGGGAG CAGGAGCTGG TCCAGCTGGG GGCCTGGGTG 
GCGCGGGACC GTGCCAGCTA CGAGGCCCGG GAGCGGCACC TGGCTGAGCG GCAACTGACC 
CACCTGGAGG AGATGCAGCA CACCATCTCT AACGATGAGG AGAAGAGCGC CGCCGTGGTC 
AACGCCATCA GCATGTACAT GCGGCACCTT GGGGTGCGGA CCAAGAGTGG GGACAAGAAG 
TCCGGGAGGA ACTTCTTTCG GAAAAAGATG ATAGGGAACC GGCGGTCAGA TGAACCGACC 
AAGACCAAGA AAGGCCTGAG CAGCTTCCTG GATGCCGCCC GCTGGAACCG GGGAGAGCCC 
CAGGATTTTC GACACCCGAA GGTCGAAGTT GATGCTGAGA AGCCAGGCCT TACAGAACGT 
AAGGGAGGTC AGGGGGCGCC TTCCCGGGAC CGGAATCTCG GAGGCCCTGG GCAGGACACC 
CCCGGAGTTT CTCCGCACCT TCTGTCTGGG GACAGCCCTG ACCGGGAGCC AGGTGTTGAC 
GCCCTCCTGG AGGTGGGGGA CCCGCCCCCA CAGGGCCCAG CCAGCCTGGA CGCCCCAGCG 
CCGCTGGAGA GCACGGAGGA GGCTGCCGAC ACAGAAAGGC TGTCAGGGCG TCTGGGGCGC 
TCAGAGAGCC TGCGGGTGAG TGACCGCCGC CGGCCTTCCC GGGGTAGCCT CGGGGCTAAG 
GGCCGGGGTG GGGGCCGCTC CCGGAGCGAC GTGGACATGG ACCCCAGCTC CGCCACGGCC 
GTGCTTGGCC CTACCCGACG AGCCACCCCG GAGCCTGGAG ATGAGGGAGA GCCGGGCCGC 
TCAGGACTGG AGCTGGAACC AGAGGAGCCG CCTGGCTGGC GAGAGCTTGT CCCCCAGGAC 
ATTCTGCACA GCCTGCCCAA GAGCCAGGTG AAGCGGCAGG AGGTCATCAG CGAGCTCCTG 
GTGACGGAGG CTGCCCATGT GCGCATGCTC CGCGTGCTCC ACGACGTCTT CTACCAGCCC 
ATAGCGGACG GGGGCTTCTA CTCCCAGGAG GAGCTCCAGA ACATCTTCCC CAGCCTGGAC 
GAGCTCATCG AGGTGCATTC CCTATTCCTT GATTCCCTGA TGAAGCGGAG GCAGGACAGT 
GGCTACCTCA TCAAGGAGAT CGGAGATGTG CTGCTGGCCC GGTTCGATGG TGCCGAGGGC 
TCGTGGTTCC AGAAAATCTC CTCCCGCTTC TGTAGCCGCC AGTCGTTCGC CTTAGAGCAG 
CTCAAAGCCA AACAGCGCAA GGAGCCCCGG TTCTGTACCT TCGTGCAGGA GGCCGAGAGC 
CGGCCCCGGT GCCGCCGGCT GCAGCTGAAG GACATGATCC CCACCGAGAT GCAGCGTCTG 
ACCAAGTACC CACTGCTTCT GCAGAGCATC GAGCAGAACA CAGAAGAGCC GGTGGAACGG 
GAGAAAGTGG AGCGGGCAGC CGAGTGCTGC CGGGAAATTC TGCAACACGT CAACCTTGCC 
GTGCGGGACA TGGAGGACCT GCTGCGGCTC AAAGATTATC AGAGGCGTCT GGATTTGACC 
CACCTGCGGC AGAGCAACGA CCCCATGCTG AGCGAGTTCA AGAACCTAGA CATCACCAAG 
AAGAGGTTGA TCTACGAGGG CCCACTGACG TGGCGGGTGA CGAAGGACAA GGCTGTGGAG 
GTCCACGTGC TGCTGCTGGA TGACCTGCTG CTGCTGCTTC AGCGCCAGGA CGAGCGGTTC 
TTGCTTAAGT CGCACAGCCG GACGCTGACG CCCACGCCCG ATGGCAAGAC CATGCTGCGG 
CCAGTGCTGC GGCTCACCTC CGCCATGACC CGCGAGGTGG CCACCGATCA CAAAGCCTTC 
TATGTCATTT TTACCTGGGA CCAGGAGGCC CAGATATACG AGCTGGTGGC GCAGACGGTG 
TCTGAGCGGA GGATCTGGTG TACCCTGATC ACCGAGACCG CTGGATGCCT GAAGGTCCCC 
TCCCGCACCT CCCGACCCAA ACACCGGCCC GGCCCCAGCA GCACTCGTGA ACCCCTGCTT 
AGCAGCTCCG AAAACGGCAA TGGCAGCCGA GAGATGCCCC CGGCAGATGG CCGGATGGAG 
AGACTCTTCA ACTCCCTCCT GCCCTTCTGC AGACAAGGCC TCGAGGGCCA GCTCGCCGCC 
AAGGCCCTTC AGAAAGTGCT GACCCTGAAA CAGCTCCTGT TGCCTTCGGA GGAAGACAGC 
GGGGCAGGGC CTCCCCTCGA AGGGGATGGG GTCCCGGGGG GCAGCCCCTT GAGCCCAACG 
CAGCCCCAGG AAATTCGGGA GGAGCTGCTC AGCCTGGAGG AGACCATTAA ACAGCTAGAG 
GAGGTGGAGG AGGAATTTTG CCGCCTGAGA CTCCTCCTGT CTCAGCTTGG GGAGAACACC 
GTCCCCCAGT CTGGCTGTAC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024834880.1
CDS	16..3279
Translation	MSGRSEKSGARALRRDRLTKPGEAGRGPGGADARRSRAERGKEGGAGGEKGKEAEVPGGRGARAGSKEGRRGWGLRKNAPTSCPARARKREPGHRARDRRARPRAPRRSPPRADTSPAVPMEAEDVARGAAPGPPRPGLAPVSIIGAEDEDFEIELKTNPEEQNNQFQNLDQVKRRPAHLMALLQHVAMQFEPGPLLCCLHADMLGSLGPKEFKKAFIDFYHSFLDKHSILRVMVPPAVAFELDRTRPDLFPEDLQRQFVQEVVQSQQVAVVRQLEDFRSKKLMGMTPWEQELVQLGAWVARDRASYEARERHLAERQLTHLEEMQHTISNDEEKSAAVVNAISMYMRHLGVRTKSGDKKSGRNFFRKKMIGNRRSDEPTKTKKGLSSFLDAARWNRGEPQDFRHPKVEVDAEKPGLTERKGGQGAPSRDRNLGGPGQDTPGVSPHLLSGDSPDREPGVDALLEVGDPPPQGPASLDAPAPLESTEEAADTERLSGRLGRSESLRVSDRRRPSRGSLGAKGRGGGRSRSDVDMDPSSATAVLGPTRRATPEPGDEGEPGRSGLELEPEEPPGWRELVPQDILHSLPKSQVKRQEVISELLVTEAAHVRMLRVLHDVFYQPIADGGFYSQEELQNIFPSLDELIEVHSLFLDSLMKRRQDSGYLIKEIGDVLLARFDGAEGSWFQKISSRFCSRQSFALEQLKAKQRKEPRFCTFVQEAESRPRCRRLQLKDMIPTEMQRLTKYPLLLQSIEQNTEEPVEREKVERAAECCREILQHVNLAVRDMEDLLRLKDYQRRLDLTHLRQSNDPMLSEFKNLDITKKRLIYEGPLTWRVTKDKAVEVHVLLLDDLLLLLQRQDERFLLKSHSRTLTPTPDGKTMLRPVLRLTSAMTREVATDHKAFYVIFTWDQEAQIYELVAQTVSERRIWCTLITETAGCLKVPSRTSRPKHRPGPSSTREPLLSSSENGNGSREMPPADGRMERLFNSLLPFCRQGLEGQLAAKALQKVLTLKQLLLPSEEDSGAGPPLEGDGVPGGSPLSPTQPQEIREELLSLEETIKQLEEVEEEFCRLRLLLSQLGENTVPQSGCT

Target ORF information:

RefSeq Version	XM_024979112.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024979112.1

ORF Insert Sequence:

ATGTCAGGCA GATCTGAGAA AAGTGGTGCC CGCGCCCTGC GGAGGGACAG ACTGACAAAG 
CCGGGAGAAG CTGGGCGGGG TCCGGGTGGG GCCGATGCCC GCAGGTCCAG GGCGGAGCGG 
GGAAAGGAGG GCGGGGCCGG CGGCGAGAAG GGGAAGGAAG CGGAGGTGCC GGGCGGGCGC 
GGGGCGCGGG CAGGAAGCAA GGAGGGGCGG CGGGGCTGGG GCCTCCGGAA AAACGCCCCA 
ACTTCCTGCC CCGCCAGAGC CAGGAAGCGG GAGCCGGGAC ACCGGGCCCG GGATCGCCGA 
GCCCGACCTC GGGCGCCCCG CCGGTCACCA CCCCGCGCGG ACACCAGCCC GGCAGTGCCC 
ATGGAGGCAG AAGACGTCGC CCGAGGGGCG GCCCCGGGGC CCCCCCGACC CGGCCTGGCG 
CCCGTCAGCA TCATTGGGGC AGAGGACGAG GATTTTGAGA TTGAGTTGAA GACGAACCCT 
GAGGAACAAA ACAACCAGTT CCAGAACCTG GATCAGGTGA AGCGGCGCCC GGCCCACCTC 
ATGGCCCTCC TGCAGCACGT GGCCATGCAG TTTGAGCCGG GACCCCTGCT CTGCTGCCTG 
CATGCGGACA TGCTGGGCTC CCTGGGCCCC AAGGAGTTCA AGAAAGCCTT CATCGACTTC 
TACCACAGCT TCCTGGATAA GCACTCGATT CTACGAGTGA TGGTCCCTCC TGCCGTGGCC 
TTTGAACTTG ACCGCACGCG GCCTGACCTC TTTCCTGAGG ATCTCCAGCG GCAGTTCGTG 
CAGGAGGTGG TGCAGAGCCA GCAGGTAGCC GTGGTCCGGC AGCTGGAGGA CTTCCGCTCC 
AAGAAGCTCA TGGGCATGAC ACCCTGGGAG CAGGAGCTGG TCCAGCTGGG GGCCTGGGTG 
GCGCGGGACC GTGCCAGCTA CGAGGCCCGG GAGCGGCACC TGGCTGAGCG GCAACTGACC 
CACCTGGAGG AGATGCAGCA CACCATCTCT AACGATGAGG AGAAGAGCGC CGCCGTGGTC 
AACGCCATCA GCATGTACAT GCGGCACCTT GGGGTGCGGA CCAAGAGTGG GGACAAGAAG 
TCCGGGAGGA ACTTCTTTCG GAAAAAGATG ATAGGGAACC GGCGGTCAGA TGAACCGACC 
AAGACCAAGA AAGGCCTGAG CAGCTTCCTG GATGCCGCCC GCTGGAACCG GGGAGAGCCC 
CAGGATTTTC GACACCCGAA GGTCGAAGTT GATGCTGAGA AGCCAGGCCT TACAGAACGT 
AAGGGAGGTC AGGGGGCGCC TTCCCGGGAC CGGAATCTCG GAGGCCCTGG GCAGGACACC 
CCCGGAGTTT CTCCGCACCT TCTGTCTGGG GACAGCCCTG ACCGGGAGCC AGGTGTTGAC 
GCCCTCCTGG AGGTGGGGGA CCCGCCCCCA CAGGGCCCAG CCAGCCTGGA CGCCCCAGCG 
CCGCTGGAGA GCACGGAGGA GGCTGCCGAC ACAGAAAGGC TGTCAGGGCG TCTGGGGCGC 
TCAGAGAGCC TGCGGGTGAG TGACCGCCGC CGGCCTTCCC GGGGTAGCCT CGGGGCTAAG 
GGCCGGGGTG GGGGCCGCTC CCGGAGCGAC GTGGACATGG ACCCCAGCTC CGCCACGGCC 
GTGCTTGGCC CTACCCGACG AGCCACCCCG GAGCCTGGAG ATGAGGGAGA GCCGGGCCGC 
TCAGGACTGG AGCTGGAACC AGAGGAGCCG CCTGGCTGGC GAGAGCTTGT CCCCCAGGAC 
ATTCTGCACA GCCTGCCCAA GAGCCAGGTG AAGCGGCAGG AGGTCATCAG CGAGCTCCTG 
GTGACGGAGG CTGCCCATGT GCGCATGCTC CGCGTGCTCC ACGACGTCTT CTACCAGCCC 
ATAGCGGACG GGGGCTTCTA CTCCCAGGAG GAGCTCCAGA ACATCTTCCC CAGCCTGGAC 
GAGCTCATCG AGGTGCATTC CCTATTCCTT GATTCCCTGA TGAAGCGGAG GCAGGACAGT 
GGCTACCTCA TCAAGGAGAT CGGAGATGTG CTGCTGGCCC GGTTCGATGG TGCCGAGGGC 
TCGTGGTTCC AGAAAATCTC CTCCCGCTTC TGTAGCCGCC AGTCGTTCGC CTTAGAGCAG 
CTCAAAGCCA AACAGCGCAA GGAGCCCCGG TTCTGTACCT TCGTGCAGGA GGCCGAGAGC 
CGGCCCCGGT GCCGCCGGCT GCAGCTGAAG GACATGATCC CCACCGAGAT GCAGCGTCTG 
ACCAAGTACC CACTGCTTCT GCAGAGCATC GAGCAGAACA CAGAAGAGCC GGTGGAACGG 
GAGAAAGTGG AGCGGGCAGC CGAGTGCTGC CGGGAAATTC TGCAACACGT CAACCTTGCC 
GTGCGGGACA TGGAGGACCT GCTGCGGCTC AAAGATTATC AGAGGCGTCT GGATTTGACC 
CACCTGCGGC AGAGCAACGA CCCCATGCTG AGCGAGTTCA AGAACCTAGA CATCACCAAG 
AAGAGGTTGA TCTACGAGGG CCCACTGACG TGGCGGGTGA CGAAGGACAA GGCTGTGGAG 
GTCCACGTGC TGCTGCTGGA TGACCTGCTG CTGCTGCTTC AGCGCCAGGA CGAGCGGTTC 
TTGCTTAAGT CGCACAGCCG GACGCTGACG CCCACGCCCG ATGGCAAGAC CATGCTGCGG 
CCAGTGCTGC GGCTCACCTC CGCCATGACC CGCGAGGTGG CCACCGATCA CAAAGCCTTC 
TATGTCATTT TTACCTGGGA CCAGGAGGCC CAGATATACG AGCTGGTGGC GCAGACGGTG 
TCTGAGCGGA GGATCTGGTG TACCCTGATC ACCGAGACCG CTGGATGCCT GAAGGTCCCC 
TCCCGCACCT CCCGACCCAA ACACCGGCCC GGCCCCAGCA GCACTCGTGA ACCCCTGCTT 
AGCAGCTCCG AAAACGGCAA TGGCAGCCGA GAGATGCCCC CGGCAGATGG CCGGATGGAG 
AGACTCTTCA ACTCCCTCCT GCCCTTCTGC AGACAAGGCC TCGAGGGCCA GCTCGCCGCC 
AAGGCCCTTC AGAAAGTGCT GACCCTGAAA CAGCTCCTGT TGCCTTCGGA GGAAGACAGC 
GGGGCAGGGC CTCCCCTCGA AGGGGATGGG GTCCCGGGGG GCAGCCCCTT GAGCCCAACG 
CAGCCCCAGG AAATTCGGGA GGAGCTGCTC AGCCTGGAGG AGACCATTAA ACAGCTAGAG 
GAGGTGGAGG AGGAATTTTG CCGCCTGAGA CTCCTCCTGT CTCAGCTTGG GGAGAACACC 
GTCCCCCAGT CTGGCTGTAC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF1 ( XM_024979113.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024834881.1 Bos taurus Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa225591
Clone ID Related Accession (Same CDS sequence)	XM_024979113.1
Accession Version	XM_024979113.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3162bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rho guanine nucleotide exchange factor 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGGCA GATCTGAGAA AAGTGGTGCC CGCGCCCTGC GGAGGGACAG ACTGACAAAG 
CCGGGAGAAG CTGGGCGGGG TCCGGGTGGG GCCGATGCCC GCAGGTCCAG GGCGGAGCGG 
GGAAAGGAGG GCGGGGCCGG CGGCGAGAAG GGGAAGGAAG CGGAGGTGCC GGGCGGGCGC 
GGGGCGCGGG CAGGAAGCAA GGAGGGGCGG CGGGGCTGGG GCCTCCGGAA AAACGCCCCA 
ACTTCCTGCC CCGCCAGAGC CAGGAAGCGG GAGCCGGGAC ACCGGGCCCG GGATCGCCGA 
GCCCGACCTC GGGCGCCCCG CCGGTCACCA CCCCGCGCGG ACACCAGCCC GGCAGTGCCC 
ATGGAGGCAG AAGACGTCGC CCGAGGGGCG GCCCCGGGGC CCCCCCGACC CGGCCTGGCG 
CCCGTCAGCA TCATTGGGGC AGAGGACGAG GATTTTGAGA TTGAGTTGAA GACGCTCTGC 
TGCCTGCATG CGGACATGCT GGGCTCCCTG GGCCCCAAGG AGTTCAAGAA AGCCTTCATC 
GACTTCTACC ACAGCTTCCT GGATAAGCAC TCGATTCTAC GAGTGATGGT CCCTCCTGCC 
GTGGCCTTTG AACTTGACCG CACGCGGCCT GACCTCTTTC CTGAGGATCT CCAGCGGCAG 
TTCGTGCAGG AGGTGGTGCA GAGCCAGCAG GTAGCCGTGG TCCGGCAGCT GGAGGACTTC 
CGCTCCAAGA AGCTCATGGG CATGACACCC TGGGAGCAGG AGCTGGTCCA GCTGGGGGCC 
TGGGTGGCGC GGGACCGTGC CAGCTACGAG GCCCGGGAGC GGCACCTGGC TGAGCGGCAA 
CTGACCCACC TGGAGGAGAT GCAGCACACC ATCTCTAACG ATGAGGAGAA GAGCGCCGCC 
GTGGTCAACG CCATCAGCAT GTACATGCGG CACCTTGGGG TGCGGACCAA GAGTGGGGAC 
AAGAAGTCCG GGAGGAACTT CTTTCGGAAA AAGATGATAG GGAACCGGCG GTCAGATGAA 
CCGACCAAGA CCAAGAAAGG CCTGAGCAGC TTCCTGGATG CCGCCCGCTG GAACCGGGGA 
GAGCCCCAGG ATTTTCGACA CCCGAAGGTC GAAGTTGATG CTGAGAAGCC AGGCCTTACA 
GAACGTAAGG GAGGTCAGGG GGCGCCTTCC CGGGACCGGA ATCTCGGAGG CCCTGGGCAG 
GACACCCCCG GAGTTTCTCC GCACCTTCTG TCTGGGGACA GCCCTGACCG GGAGCCAGGT 
GTTGACGCCC TCCTGGAGGT GGGGGACCCG CCCCCACAGG GCCCAGCCAG CCTGGACGCC 
CCAGCGCCGC TGGAGAGCAC GGAGGAGGCT GCCGACACAG AAAGAAAGTG GAAGAGGCTG 
TCAGGGCGTC TGGGGCGCTC AGAGAGCCTG CGGGTGAGTG ACCGCCGCCG GCCTTCCCGG 
GGTAGCCTCG GGGCTAAGGG CCGGGGTGGG GGCCGCTCCC GGAGCGACGT GGACATGGAC 
CCCAGCTCCG CCACGGCCGT GCTTGGCCCT ACCCGACGAG CCACCCCGGA GCCTGGAGAT 
GAGGGAGAGC CGGGCCGCTC AGGACTGGAG CTGGAACCAG AGGAGCCGCC TGGCTGGCGA 
GAGCTTGTCC CCCAGGACAT TCTGCACAGC CTGCCCAAGA GCCAGGTGAA GCGGCAGGAG 
GTCATCAGCG AGCTCCTGGT GACGGAGGCT GCCCATGTGC GCATGCTCCG CGTGCTCCAC 
GACGTCTTCT ACCAGCCCAT AGCGGACGGG GGCTTCTACT CCCAGGAGGA GCTCCAGAAC 
ATCTTCCCCA GCCTGGACGA GCTCATCGAG GTGCATTCCC TATTCCTTGA TTCCCTGATG 
AAGCGGAGGC AGGACAGTGG CTACCTCATC AAGGAGATCG GAGATGTGCT GCTGGCCCGG 
TTCGATGGTG CCGAGGGCTC GTGGTTCCAG AAAATCTCCT CCCGCTTCTG TAGCCGCCAG 
TCGTTCGCCT TAGAGCAGCT CAAAGCCAAA CAGCGCAAGG AGCCCCGGTT CTGTACCTTC 
GTGCAGGAGG CCGAGAGCCG GCCCCGGTGC CGCCGGCTGC AGCTGAAGGA CATGATCCCC 
ACCGAGATGC AGCGTCTGAC CAAGTACCCA CTGCTTCTGC AGAGCATCGA GCAGAACACA 
GAAGAGCCGG TGGAACGGGA GAAAGTGGAG CGGGCAGCCG AGTGCTGCCG GGAAATTCTG 
CAACACGTCA ACCTTGCCGT GCGGGACATG GAGGACCTGC TGCGGCTCAA AGATTATCAG 
AGGCGTCTGG ATTTGACCCA CCTGCGGCAG AGCAACGACC CCATGCTGAG CGAGTTCAAG 
AACCTAGACA TCACCAAGAA GAGGTTGATC TACGAGGGCC CACTGACGTG GCGGGTGACG 
AAGGACAAGG CTGTGGAGGT CCACGTGCTG CTGCTGGATG ACCTGCTGCT GCTGCTTCAG 
CGCCAGGACG AGCGGTTCTT GCTTAAGTCG CACAGCCGGA CGCTGACGCC CACGCCCGAT 
GGCAAGACCA TGCTGCGGCC AGTGCTGCGG CTCACCTCCG CCATGACCCG CGAGGTGGCC 
ACCGATCACA AAGCCTTCTA TGTCATTTTT ACCTGGGACC AGGAGGCCCA GATATACGAG 
CTGGTGGCGC AGACGGTGTC TGAGCGGAGG ATCTGGTGTA CCCTGATCAC CGAGACCGCT 
GGATGCCTGA AGGTCCCCTC CCGCACCTCC CGACCCAAAC ACCGGCCCGG CCCCAGCAGC 
ACTCGTGAAC CCCTGCTTAG CAGCTCCGAA AACGGCAATG GCAGCCGAGA GATGCCCCCG 
GCAGATGGCC GGATGGAGAG ACTCTTCAAC TCCCTCCTGC CCTTCTGCAG ACAAGGCCTC 
GAGGGCCAGC TCGCCGCCAA GGCCCTTCAG AAAGTGCTGA CCCTGAAACA GCTCCTGTTG 
CCTTCGGAGG AAGACAGCGG GGCAGGGCCT CCCCTCGAAG GGGATGGGGT CCCGGGGGGC 
AGCCCCTTGA GCCCAACGCA GCCCCAGGAA ATTCGGGAGG AGCTGCTCAG CCTGGAGGAG 
ACCATTAAAC AGCTAGAGGA GGTGGAGGAG GAATTTTGCC GCCTGAGACT CCTCCTGTCT 
CAGCTTGGGG AGAACACCGT CCCCCAGTCT GGCTGTACCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024834881.1
CDS	16..3177
Translation	MSGRSEKSGARALRRDRLTKPGEAGRGPGGADARRSRAERGKEGGAGGEKGKEAEVPGGRGARAGSKEGRRGWGLRKNAPTSCPARARKREPGHRARDRRARPRAPRRSPPRADTSPAVPMEAEDVARGAAPGPPRPGLAPVSIIGAEDEDFEIELKTLCCLHADMLGSLGPKEFKKAFIDFYHSFLDKHSILRVMVPPAVAFELDRTRPDLFPEDLQRQFVQEVVQSQQVAVVRQLEDFRSKKLMGMTPWEQELVQLGAWVARDRASYEARERHLAERQLTHLEEMQHTISNDEEKSAAVVNAISMYMRHLGVRTKSGDKKSGRNFFRKKMIGNRRSDEPTKTKKGLSSFLDAARWNRGEPQDFRHPKVEVDAEKPGLTERKGGQGAPSRDRNLGGPGQDTPGVSPHLLSGDSPDREPGVDALLEVGDPPPQGPASLDAPAPLESTEEAADTERKWKRLSGRLGRSESLRVSDRRRPSRGSLGAKGRGGGRSRSDVDMDPSSATAVLGPTRRATPEPGDEGEPGRSGLELEPEEPPGWRELVPQDILHSLPKSQVKRQEVISELLVTEAAHVRMLRVLHDVFYQPIADGGFYSQEELQNIFPSLDELIEVHSLFLDSLMKRRQDSGYLIKEIGDVLLARFDGAEGSWFQKISSRFCSRQSFALEQLKAKQRKEPRFCTFVQEAESRPRCRRLQLKDMIPTEMQRLTKYPLLLQSIEQNTEEPVEREKVERAAECCREILQHVNLAVRDMEDLLRLKDYQRRLDLTHLRQSNDPMLSEFKNLDITKKRLIYEGPLTWRVTKDKAVEVHVLLLDDLLLLLQRQDERFLLKSHSRTLTPTPDGKTMLRPVLRLTSAMTREVATDHKAFYVIFTWDQEAQIYELVAQTVSERRIWCTLITETAGCLKVPSRTSRPKHRPGPSSTREPLLSSSENGNGSREMPPADGRMERLFNSLLPFCRQGLEGQLAAKALQKVLTLKQLLLPSEEDSGAGPPLEGDGVPGGSPLSPTQPQEIREELLSLEETIKQLEEVEEEFCRLRLLLSQLGENTVPQSGCT

Target ORF information:

RefSeq Version	XM_024979113.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024979113.1

ORF Insert Sequence:

ATGTCAGGCA GATCTGAGAA AAGTGGTGCC CGCGCCCTGC GGAGGGACAG ACTGACAAAG 
CCGGGAGAAG CTGGGCGGGG TCCGGGTGGG GCCGATGCCC GCAGGTCCAG GGCGGAGCGG 
GGAAAGGAGG GCGGGGCCGG CGGCGAGAAG GGGAAGGAAG CGGAGGTGCC GGGCGGGCGC 
GGGGCGCGGG CAGGAAGCAA GGAGGGGCGG CGGGGCTGGG GCCTCCGGAA AAACGCCCCA 
ACTTCCTGCC CCGCCAGAGC CAGGAAGCGG GAGCCGGGAC ACCGGGCCCG GGATCGCCGA 
GCCCGACCTC GGGCGCCCCG CCGGTCACCA CCCCGCGCGG ACACCAGCCC GGCAGTGCCC 
ATGGAGGCAG AAGACGTCGC CCGAGGGGCG GCCCCGGGGC CCCCCCGACC CGGCCTGGCG 
CCCGTCAGCA TCATTGGGGC AGAGGACGAG GATTTTGAGA TTGAGTTGAA GACGCTCTGC 
TGCCTGCATG CGGACATGCT GGGCTCCCTG GGCCCCAAGG AGTTCAAGAA AGCCTTCATC 
GACTTCTACC ACAGCTTCCT GGATAAGCAC TCGATTCTAC GAGTGATGGT CCCTCCTGCC 
GTGGCCTTTG AACTTGACCG CACGCGGCCT GACCTCTTTC CTGAGGATCT CCAGCGGCAG 
TTCGTGCAGG AGGTGGTGCA GAGCCAGCAG GTAGCCGTGG TCCGGCAGCT GGAGGACTTC 
CGCTCCAAGA AGCTCATGGG CATGACACCC TGGGAGCAGG AGCTGGTCCA GCTGGGGGCC 
TGGGTGGCGC GGGACCGTGC CAGCTACGAG GCCCGGGAGC GGCACCTGGC TGAGCGGCAA 
CTGACCCACC TGGAGGAGAT GCAGCACACC ATCTCTAACG ATGAGGAGAA GAGCGCCGCC 
GTGGTCAACG CCATCAGCAT GTACATGCGG CACCTTGGGG TGCGGACCAA GAGTGGGGAC 
AAGAAGTCCG GGAGGAACTT CTTTCGGAAA AAGATGATAG GGAACCGGCG GTCAGATGAA 
CCGACCAAGA CCAAGAAAGG CCTGAGCAGC TTCCTGGATG CCGCCCGCTG GAACCGGGGA 
GAGCCCCAGG ATTTTCGACA CCCGAAGGTC GAAGTTGATG CTGAGAAGCC AGGCCTTACA 
GAACGTAAGG GAGGTCAGGG GGCGCCTTCC CGGGACCGGA ATCTCGGAGG CCCTGGGCAG 
GACACCCCCG GAGTTTCTCC GCACCTTCTG TCTGGGGACA GCCCTGACCG GGAGCCAGGT 
GTTGACGCCC TCCTGGAGGT GGGGGACCCG CCCCCACAGG GCCCAGCCAG CCTGGACGCC 
CCAGCGCCGC TGGAGAGCAC GGAGGAGGCT GCCGACACAG AAAGAAAGTG GAAGAGGCTG 
TCAGGGCGTC TGGGGCGCTC AGAGAGCCTG CGGGTGAGTG ACCGCCGCCG GCCTTCCCGG 
GGTAGCCTCG GGGCTAAGGG CCGGGGTGGG GGCCGCTCCC GGAGCGACGT GGACATGGAC 
CCCAGCTCCG CCACGGCCGT GCTTGGCCCT ACCCGACGAG CCACCCCGGA GCCTGGAGAT 
GAGGGAGAGC CGGGCCGCTC AGGACTGGAG CTGGAACCAG AGGAGCCGCC TGGCTGGCGA 
GAGCTTGTCC CCCAGGACAT TCTGCACAGC CTGCCCAAGA GCCAGGTGAA GCGGCAGGAG 
GTCATCAGCG AGCTCCTGGT GACGGAGGCT GCCCATGTGC GCATGCTCCG CGTGCTCCAC 
GACGTCTTCT ACCAGCCCAT AGCGGACGGG GGCTTCTACT CCCAGGAGGA GCTCCAGAAC 
ATCTTCCCCA GCCTGGACGA GCTCATCGAG GTGCATTCCC TATTCCTTGA TTCCCTGATG 
AAGCGGAGGC AGGACAGTGG CTACCTCATC AAGGAGATCG GAGATGTGCT GCTGGCCCGG 
TTCGATGGTG CCGAGGGCTC GTGGTTCCAG AAAATCTCCT CCCGCTTCTG TAGCCGCCAG 
TCGTTCGCCT TAGAGCAGCT CAAAGCCAAA CAGCGCAAGG AGCCCCGGTT CTGTACCTTC 
GTGCAGGAGG CCGAGAGCCG GCCCCGGTGC CGCCGGCTGC AGCTGAAGGA CATGATCCCC 
ACCGAGATGC AGCGTCTGAC CAAGTACCCA CTGCTTCTGC AGAGCATCGA GCAGAACACA 
GAAGAGCCGG TGGAACGGGA GAAAGTGGAG CGGGCAGCCG AGTGCTGCCG GGAAATTCTG 
CAACACGTCA ACCTTGCCGT GCGGGACATG GAGGACCTGC TGCGGCTCAA AGATTATCAG 
AGGCGTCTGG ATTTGACCCA CCTGCGGCAG AGCAACGACC CCATGCTGAG CGAGTTCAAG 
AACCTAGACA TCACCAAGAA GAGGTTGATC TACGAGGGCC CACTGACGTG GCGGGTGACG 
AAGGACAAGG CTGTGGAGGT CCACGTGCTG CTGCTGGATG ACCTGCTGCT GCTGCTTCAG 
CGCCAGGACG AGCGGTTCTT GCTTAAGTCG CACAGCCGGA CGCTGACGCC CACGCCCGAT 
GGCAAGACCA TGCTGCGGCC AGTGCTGCGG CTCACCTCCG CCATGACCCG CGAGGTGGCC 
ACCGATCACA AAGCCTTCTA TGTCATTTTT ACCTGGGACC AGGAGGCCCA GATATACGAG 
CTGGTGGCGC AGACGGTGTC TGAGCGGAGG ATCTGGTGTA CCCTGATCAC CGAGACCGCT 
GGATGCCTGA AGGTCCCCTC CCGCACCTCC CGACCCAAAC ACCGGCCCGG CCCCAGCAGC 
ACTCGTGAAC CCCTGCTTAG CAGCTCCGAA AACGGCAATG GCAGCCGAGA GATGCCCCCG 
GCAGATGGCC GGATGGAGAG ACTCTTCAAC TCCCTCCTGC CCTTCTGCAG ACAAGGCCTC 
GAGGGCCAGC TCGCCGCCAA GGCCCTTCAG AAAGTGCTGA CCCTGAAACA GCTCCTGTTG 
CCTTCGGAGG AAGACAGCGG GGCAGGGCCT CCCCTCGAAG GGGATGGGGT CCCGGGGGGC 
AGCCCCTTGA GCCCAACGCA GCCCCAGGAA ATTCGGGAGG AGCTGCTCAG CCTGGAGGAG 
ACCATTAAAC AGCTAGAGGA GGTGGAGGAG GAATTTTGCC GCCTGAGACT CCTCCTGTCT 
CAGCTTGGGG AGAACACCGT CCCCCAGTCT GGCTGTACCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF1 ( XM_024979114.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024834882.1 Bos taurus Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa225592
Clone ID Related Accession (Same CDS sequence)	XM_024979114.1
Accession Version	XM_024979114.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3096bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rho guanine nucleotide exchange factor 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGGCA GATCTGAGAA AAGTGGTGCC CGCGCCCTGC GGAGGGACAG ACTGACAAAG 
CCGGGAGAAG CTGGGCGGGG TCCGGGTGGG GCCGATGCCC GCAGGTCCAG GGCGGAGCGG 
GGAAAGGAGG GCGGGGCCGG CGGCGAGAAG GGGAAGGAAG CGGAGGTGCC GGGCGGGCGC 
GGGGCGCGGG CAGGAAGCAA GGAGGGGCGG CGGGGCTGGG GCCTCCGGAA AAACGCCCCA 
ACTTCCTGCC CCGCCAGAGC CAGGAAGCGG GAGCCGGGAC ACCGGGCCCG GGATCGCCGA 
GCCCGACCTC GGGCGCCCCG CCGGTCACCA CCCCGCGCGG ACACCAGCCC GGCAGTGCCC 
ATGGAGGCAG AAGACGTCGC CCGAGGGGCG GCCCCGGGGC CCCCCCGACC CGGCCTGGCG 
CCCGTCAGCA TCATTGGGGC AGAGGACGAG GATTTTGAGA TTGAGTTGAA GACGAACCCT 
GAGGAACAAA ACAACCAGTT CCAGAACCTG GATCAGGTGA AGCGGCGCCC GGCCCACCTC 
ATGGCCCTCC TGCAGCACGT GGCCATGCAG TTTGAGCCGG GACCCCTGCT CTGCTGCCTG 
CATGCGGACA TGCTGGGCTC CCTGGGCCCC AAGGAGTTCA AGAAAGCCTT CATCGACTTC 
TACCACAGCT TCCTGGATAA GCACTCGATT CTACGAGTGA TGGTCCCTCC TGCCGTGGCC 
TTTGAACTTG ACCGCACGCG GCCTGACCTC TTTCCTGAGG ATCTCCAGCG GCAGTTCGTG 
CAGGAGGTGG TGCAGAGCCA GCAGGTAGCC GTGGTCCGGC AGCTGGAGGA CTTCCGCTCC 
AAGAAGCTCA TGGGCATGAC ACCCTGGGAG CAGGAGCTGG TCCAGCTGGG GGCCTGGGTG 
GCGCGGGACC GTGCCAGCTA CGAGGCCCGG GAGCGGCACC TGGCTGAGCG GCAACTGACC 
CACCTGGAGG AGATGCAGCA CACCATCTCT AACGATGAGG AGAAGAGCGC CGCCGTGGTC 
AACGCCATCA GCATGTACAT GCGGCACCTT GGGGTGCGGA CCAAGAGTGG GGACAAGAAG 
TCCGGGAGGA ACTTCTTTCG GAAAAAGATG ATAGGGAACC GGCGGTCAGA TGAACCGACC 
AAGACCAAGA AAGGCCTGAG CAGCTTCCTG GATGCCGCCC GCTGGAACCG GGGAGAGCCC 
CAGGATTTTC GACACCCGAA GGTCGAAGTT GATGCTGAGA AGCCAGGCCT TACAGAACGT 
AAGGGAGGTC AGGGGGCGCC TTCCCGGGAC CGGAATCTCG GAGGCCCTGG GCAGGACACC 
CCCGGAGTTT CTCCGCACCT TCTGTCTGGG GACAGCCCTG ACCGGGAGCC AGGTGTTGAC 
GCCCTCCTGG AGGTGGGGGA CCCGCCCCCA CAGGGCCCAG CCAGCCTGGA CGCCCCAGCG 
CCGCTGGAGA GCACGGAGGA GGCTGCCGAC ACAGAAAGCC CGGAGCCTGG AGATGAGGGA 
GAGCCGGGCC GCTCAGGACT GGAGCTGGAA CCAGAGGAGC CGCCTGGCTG GCGAGAGCTT 
GTCCCCCAGG ACATTCTGCA CAGCCTGCCC AAGAGCCAGG TGAAGCGGCA GGAGGTCATC 
AGCGAGCTCC TGGTGACGGA GGCTGCCCAT GTGCGCATGC TCCGCGTGCT CCACGACGTC 
TTCTACCAGC CCATAGCGGA CGGGGGCTTC TACTCCCAGG AGGAGCTCCA GAACATCTTC 
CCCAGCCTGG ACGAGCTCAT CGAGGTGCAT TCCCTATTCC TTGATTCCCT GATGAAGCGG 
AGGCAGGACA GTGGCTACCT CATCAAGGAG ATCGGAGATG TGCTGCTGGC CCGGTTCGAT 
GGTGCCGAGG GCTCGTGGTT CCAGAAAATC TCCTCCCGCT TCTGTAGCCG CCAGTCGTTC 
GCCTTAGAGC AGCTCAAAGC CAAACAGCGC AAGGAGCCCC GGTTCTGTAC CTTCGTGCAG 
GAGGCCGAGA GCCGGCCCCG GTGCCGCCGG CTGCAGCTGA AGGACATGAT CCCCACCGAG 
ATGCAGCGTC TGACCAAGTA CCCACTGCTT CTGCAGAGCA TCGAGCAGAA CACAGAAGAG 
CCGGTGGAAC GGGAGAAAGT GGAGCGGGCA GCCGAGTGCT GCCGGGAAAT TCTGCAACAC 
GTCAACCTTG CCGTGCGGGA CATGGAGGAC CTGCTGCGGC TCAAAGATTA TCAGAGGCGT 
CTGGATTTGA CCCACCTGCG GCAGAGCAAC GACCCCATGC TGAGCGAGTT CAAGAACCTA 
GACATCACCA AGAAGAGGTT GATCTACGAG GGCCCACTGA CGTGGCGGGT GACGAAGGAC 
AAGGCTGTGG AGGTCCACGT GCTGCTGCTG GATGACCTGC TGCTGCTGCT TCAGCGCCAG 
GACGAGCGGT TCTTGCTTAA GTCGCACAGC CGGACGCTGA CGCCCACGCC CGATGGCAAG 
ACCATGCTGC GGCCAGTGCT GCGGCTCACC TCCGCCATGA CCCGCGAGGT GGCCACCGAT 
CACAAAGCCT TCTATGTCAT TTTTACCTGG GACCAGGAGG CCCAGATATA CGAGCTGGTG 
GCGCAGACGG TGTCTGAGCG GAGGATCTGG TGTACCCTGA TCACCGAGAC CGCTGGATGC 
CTGAAGGTCC CCTCCCGCAC CTCCCGACCC AAACACCGGC CCGGCCCCAG CAGCACTCGT 
GAACCCCTGC TTAGCAGCTC CGAAAACGGC AATGGCAGCC GAGAGATGCC CCCGGCAGAT 
GGCCGGATGG AGAGACTCTT CAACTCCCTC CTGCCCTTCT GCAGACAAGG CCTCGAGGGC 
CAGCTCGCCG CCAAGGCCCT TCAGAAAGTG CTGACCCTGA AACAGCTCCT GTTGCCTTCG 
GAGGAAGACA GCGGGGCAGG GCCTCCCCTC GAAGGGGATG GGGTCCCGGG GGGCAGCCCC 
TTGAGCCCAA CGCAGCCCCA GGAAATTCGG GAGGAGCTGC TCAGCCTGGA GGAGACCATT 
AAACAGCTAG AGGAGGTGGA GGAGGAATTT TGCCGCCTGA GACTCCTCCT GTCTCAGCTT 
GGGGAGAACA CCGTCCCCCA GTCTGGCTGT ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024834882.1
CDS	16..3111
Translation	MSGRSEKSGARALRRDRLTKPGEAGRGPGGADARRSRAERGKEGGAGGEKGKEAEVPGGRGARAGSKEGRRGWGLRKNAPTSCPARARKREPGHRARDRRARPRAPRRSPPRADTSPAVPMEAEDVARGAAPGPPRPGLAPVSIIGAEDEDFEIELKTNPEEQNNQFQNLDQVKRRPAHLMALLQHVAMQFEPGPLLCCLHADMLGSLGPKEFKKAFIDFYHSFLDKHSILRVMVPPAVAFELDRTRPDLFPEDLQRQFVQEVVQSQQVAVVRQLEDFRSKKLMGMTPWEQELVQLGAWVARDRASYEARERHLAERQLTHLEEMQHTISNDEEKSAAVVNAISMYMRHLGVRTKSGDKKSGRNFFRKKMIGNRRSDEPTKTKKGLSSFLDAARWNRGEPQDFRHPKVEVDAEKPGLTERKGGQGAPSRDRNLGGPGQDTPGVSPHLLSGDSPDREPGVDALLEVGDPPPQGPASLDAPAPLESTEEAADTESPEPGDEGEPGRSGLELEPEEPPGWRELVPQDILHSLPKSQVKRQEVISELLVTEAAHVRMLRVLHDVFYQPIADGGFYSQEELQNIFPSLDELIEVHSLFLDSLMKRRQDSGYLIKEIGDVLLARFDGAEGSWFQKISSRFCSRQSFALEQLKAKQRKEPRFCTFVQEAESRPRCRRLQLKDMIPTEMQRLTKYPLLLQSIEQNTEEPVEREKVERAAECCREILQHVNLAVRDMEDLLRLKDYQRRLDLTHLRQSNDPMLSEFKNLDITKKRLIYEGPLTWRVTKDKAVEVHVLLLDDLLLLLQRQDERFLLKSHSRTLTPTPDGKTMLRPVLRLTSAMTREVATDHKAFYVIFTWDQEAQIYELVAQTVSERRIWCTLITETAGCLKVPSRTSRPKHRPGPSSTREPLLSSSENGNGSREMPPADGRMERLFNSLLPFCRQGLEGQLAAKALQKVLTLKQLLLPSEEDSGAGPPLEGDGVPGGSPLSPTQPQEIREELLSLEETIKQLEEVEEEFCRLRLLLSQLGENTVPQSGCT

Target ORF information:

RefSeq Version	XM_024979114.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024979114.1

ORF Insert Sequence:

ATGTCAGGCA GATCTGAGAA AAGTGGTGCC CGCGCCCTGC GGAGGGACAG ACTGACAAAG 
CCGGGAGAAG CTGGGCGGGG TCCGGGTGGG GCCGATGCCC GCAGGTCCAG GGCGGAGCGG 
GGAAAGGAGG GCGGGGCCGG CGGCGAGAAG GGGAAGGAAG CGGAGGTGCC GGGCGGGCGC 
GGGGCGCGGG CAGGAAGCAA GGAGGGGCGG CGGGGCTGGG GCCTCCGGAA AAACGCCCCA 
ACTTCCTGCC CCGCCAGAGC CAGGAAGCGG GAGCCGGGAC ACCGGGCCCG GGATCGCCGA 
GCCCGACCTC GGGCGCCCCG CCGGTCACCA CCCCGCGCGG ACACCAGCCC GGCAGTGCCC 
ATGGAGGCAG AAGACGTCGC CCGAGGGGCG GCCCCGGGGC CCCCCCGACC CGGCCTGGCG 
CCCGTCAGCA TCATTGGGGC AGAGGACGAG GATTTTGAGA TTGAGTTGAA GACGAACCCT 
GAGGAACAAA ACAACCAGTT CCAGAACCTG GATCAGGTGA AGCGGCGCCC GGCCCACCTC 
ATGGCCCTCC TGCAGCACGT GGCCATGCAG TTTGAGCCGG GACCCCTGCT CTGCTGCCTG 
CATGCGGACA TGCTGGGCTC CCTGGGCCCC AAGGAGTTCA AGAAAGCCTT CATCGACTTC 
TACCACAGCT TCCTGGATAA GCACTCGATT CTACGAGTGA TGGTCCCTCC TGCCGTGGCC 
TTTGAACTTG ACCGCACGCG GCCTGACCTC TTTCCTGAGG ATCTCCAGCG GCAGTTCGTG 
CAGGAGGTGG TGCAGAGCCA GCAGGTAGCC GTGGTCCGGC AGCTGGAGGA CTTCCGCTCC 
AAGAAGCTCA TGGGCATGAC ACCCTGGGAG CAGGAGCTGG TCCAGCTGGG GGCCTGGGTG 
GCGCGGGACC GTGCCAGCTA CGAGGCCCGG GAGCGGCACC TGGCTGAGCG GCAACTGACC 
CACCTGGAGG AGATGCAGCA CACCATCTCT AACGATGAGG AGAAGAGCGC CGCCGTGGTC 
AACGCCATCA GCATGTACAT GCGGCACCTT GGGGTGCGGA CCAAGAGTGG GGACAAGAAG 
TCCGGGAGGA ACTTCTTTCG GAAAAAGATG ATAGGGAACC GGCGGTCAGA TGAACCGACC 
AAGACCAAGA AAGGCCTGAG CAGCTTCCTG GATGCCGCCC GCTGGAACCG GGGAGAGCCC 
CAGGATTTTC GACACCCGAA GGTCGAAGTT GATGCTGAGA AGCCAGGCCT TACAGAACGT 
AAGGGAGGTC AGGGGGCGCC TTCCCGGGAC CGGAATCTCG GAGGCCCTGG GCAGGACACC 
CCCGGAGTTT CTCCGCACCT TCTGTCTGGG GACAGCCCTG ACCGGGAGCC AGGTGTTGAC 
GCCCTCCTGG AGGTGGGGGA CCCGCCCCCA CAGGGCCCAG CCAGCCTGGA CGCCCCAGCG 
CCGCTGGAGA GCACGGAGGA GGCTGCCGAC ACAGAAAGCC CGGAGCCTGG AGATGAGGGA 
GAGCCGGGCC GCTCAGGACT GGAGCTGGAA CCAGAGGAGC CGCCTGGCTG GCGAGAGCTT 
GTCCCCCAGG ACATTCTGCA CAGCCTGCCC AAGAGCCAGG TGAAGCGGCA GGAGGTCATC 
AGCGAGCTCC TGGTGACGGA GGCTGCCCAT GTGCGCATGC TCCGCGTGCT CCACGACGTC 
TTCTACCAGC CCATAGCGGA CGGGGGCTTC TACTCCCAGG AGGAGCTCCA GAACATCTTC 
CCCAGCCTGG ACGAGCTCAT CGAGGTGCAT TCCCTATTCC TTGATTCCCT GATGAAGCGG 
AGGCAGGACA GTGGCTACCT CATCAAGGAG ATCGGAGATG TGCTGCTGGC CCGGTTCGAT 
GGTGCCGAGG GCTCGTGGTT CCAGAAAATC TCCTCCCGCT TCTGTAGCCG CCAGTCGTTC 
GCCTTAGAGC AGCTCAAAGC CAAACAGCGC AAGGAGCCCC GGTTCTGTAC CTTCGTGCAG 
GAGGCCGAGA GCCGGCCCCG GTGCCGCCGG CTGCAGCTGA AGGACATGAT CCCCACCGAG 
ATGCAGCGTC TGACCAAGTA CCCACTGCTT CTGCAGAGCA TCGAGCAGAA CACAGAAGAG 
CCGGTGGAAC GGGAGAAAGT GGAGCGGGCA GCCGAGTGCT GCCGGGAAAT TCTGCAACAC 
GTCAACCTTG CCGTGCGGGA CATGGAGGAC CTGCTGCGGC TCAAAGATTA TCAGAGGCGT 
CTGGATTTGA CCCACCTGCG GCAGAGCAAC GACCCCATGC TGAGCGAGTT CAAGAACCTA 
GACATCACCA AGAAGAGGTT GATCTACGAG GGCCCACTGA CGTGGCGGGT GACGAAGGAC 
AAGGCTGTGG AGGTCCACGT GCTGCTGCTG GATGACCTGC TGCTGCTGCT TCAGCGCCAG 
GACGAGCGGT TCTTGCTTAA GTCGCACAGC CGGACGCTGA CGCCCACGCC CGATGGCAAG 
ACCATGCTGC GGCCAGTGCT GCGGCTCACC TCCGCCATGA CCCGCGAGGT GGCCACCGAT 
CACAAAGCCT TCTATGTCAT TTTTACCTGG GACCAGGAGG CCCAGATATA CGAGCTGGTG 
GCGCAGACGG TGTCTGAGCG GAGGATCTGG TGTACCCTGA TCACCGAGAC CGCTGGATGC 
CTGAAGGTCC CCTCCCGCAC CTCCCGACCC AAACACCGGC CCGGCCCCAG CAGCACTCGT 
GAACCCCTGC TTAGCAGCTC CGAAAACGGC AATGGCAGCC GAGAGATGCC CCCGGCAGAT 
GGCCGGATGG AGAGACTCTT CAACTCCCTC CTGCCCTTCT GCAGACAAGG CCTCGAGGGC 
CAGCTCGCCG CCAAGGCCCT TCAGAAAGTG CTGACCCTGA AACAGCTCCT GTTGCCTTCG 
GAGGAAGACA GCGGGGCAGG GCCTCCCCTC GAAGGGGATG GGGTCCCGGG GGGCAGCCCC 
TTGAGCCCAA CGCAGCCCCA GGAAATTCGG GAGGAGCTGC TCAGCCTGGA GGAGACCATT 
AAACAGCTAG AGGAGGTGGA GGAGGAATTT TGCCGCCTGA GACTCCTCCT GTCTCAGCTT 
GGGGAGAACA CCGTCCCCCA GTCTGGCTGT ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF1 ( XM_010823706.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010822008.1 Bos taurus Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa09522
Clone ID Related Accession (Same CDS sequence)	XM_010823706.2 , XM_005195333.3 , XM_005219394.3 , XM_010815120.2 , XM_005219395.3 , XM_005219394.4 , XM_005219395.4 , XM_005195332.3
Accession Version	XM_010823706.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2916bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	rho guanine nucleotide exchange factor 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646390.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010823706.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCAG AAGACGTCGC CCGAGGGGCG GCCCCGGGGC CCCCCCGACC CGGCCTGGCG 
CCCGTCAGCA TCATTGGGGC AGAGGACGAG GATTTTGAGA TTGAGTTGAA GACGAACCCT 
GAGGAACAAA ACAACCAGTT CCAGAACCTG GATCAGGTGA AGCGGCGCCC GGCCCACCTC 
ATGGCCCTCC TGCAGCACGT GGCCATGCAG TTTGAGCCGG GACCCCTGCT CTGCTGCCTG 
CATGCGGACA TGCTGGGCTC CCTGGGCCCC AAGGAGTTCA AGAAAGCCTT CATCGACTTC 
TACCACAGCT TCCTGGATAA GCACTCGATT CTACGAGTGA TGGTCCCTCC TGCCGTGGCC 
TTTGAACTTG ACCGCACGCG GCCTGACCTC TTTCCTGAGG ATCTCCAGCG GCAGTTCGTG 
CAGGAGGTGG TGCAGAGCCA GCAGGTAGCC GTGGTCCGGC AGCTGGAGGA CTTCCGCTCC 
AAGAAGCTCA TGGGCATGAC ACCCTGGGAG CAGGAGCTGG TCCAGCTGGG GGCCTGGGTG 
GCGCGGGACC GTGCCAGCTA CGAGGCCCGG GAGCGGCACC TGGCTGAGCG GCAACTGACC 
CACCTGGAGG AGATGCAGCA CACCATCTCT AACGATGAGG AGAAGAGCGC CGCCGTGGTC 
AACGCCATCA GCATGTACAT GCGGCACCTT GGGGTGCGGA CCAAGAGTGG GGACAAGAAG 
TCCGGGAGGA ACTTCTTTCG GAAAAAGATG ATAGGGAACC GGCGGTCAGA TGAACCGACC 
AAGACCAAGA AAGGCCTGAG CAGCTTCCTG GATGCCGCCC GCTGGAACCG GGGAGAGCCC 
CAGGATTTTC GACACCCGAA GGTCGAAGTT GATGCTGAGA AGCCAGGCCT TACAGAACGT 
AAGGGAGGTC AGGGGGCGCC TTCCCGGGAC CGGAATCTCG GAGGCCCTGG GCAGGACACC 
CCCGGAGTTT CTCCGCACCT TCTGTCTGGG GACAGCCCTG ACCGGGAGCC AGGTGTTGAC 
GCCCTCCTGG AGGTGGGGGA CCCGCCCCCA CAGGGCCCAG CCAGCCTGGA CGCCCCAGCG 
CCGCTGGAGA GCACGGAGGA GGCTGCCGAC ACAGAAAGAA AGTGGAAGAG GCTGTCAGGG 
CGTCTGGGGC GCTCAGAGAG CCTGCGGGTG AGTGACCGCC GCCGGCCTTC CCGGGGTAGC 
CTCGGGGCTA AGGGCCGGGG TGGGGGCCGC TCCCGGAGCG ACGTGGACAT GGACCCCAGC 
TCCGCCACGG CCGTGCTTGG CCCTACCCGA CGAGCCACCC CGGAGCCTGG AGATGAGGGA 
GAGCCGGGCC GCTCAGGACT GGAGCTGGAA CCAGAGGAGC CGCCTGGCTG GCGAGAGCTT 
GTCCCCCAGG ACATTCTGCA CAGCCTGCCC AAGAGCCAGG TGAAGCGGCA GGAGGTCATC 
AGCGAGCTCC TGGTGACGGA GGCTGCCCAT GTGCGCATGC TCCGCGTGCT CCACGACGTC 
TTCTACCAGC CCATAGCGGA CGGGGGCTTC TACTCCCAGG AGGAGCTCCA GAACATCTTC 
CCCAGCCTGG ACGAGCTCAT CGAGGTGCAT TCCCTATTCC TTGATTCCCT GATGAAGCGG 
AGGCAGGACA GTGGCTACCT CATCAAGGAG ATCGGAGATG TGCTGCTGGC CCGGTTCGAT 
GGTGCCGAGG GCTCGTGGTT CCAGAAAATC TCCTCCCGCT TCTGTAGCCG CCAGTCGTTC 
GCCTTAGAGC AGCTCAAAGC CAAACAGCGC AAGGAGCCCC GGTTCTGTAC CTTCGTGCAG 
GAGGCCGAGA GCCGGCCCCG GTGCCGCCGG CTGCAGCTGA AGGACATGAT CCCCACCGAG 
ATGCAGCGTC TGACCAAGTA CCCACTGCTT CTGCAGAGCA TCGAGCAGAA CACAGAAGAG 
CCGGTGGAAC GGGAGAAAGT GGAGCGGGCA GCCGAGTGCT GCCGGGAAAT TCTGCAACAC 
GTCAACCTTG CCGTGCGGGA CATGGAGGAC CTGCTGCGGC TCAAAGATTA TCAGAGGCGT 
CTGGATTTGA CCCACCTGCG GCAGAGCAAC GACCCCATGC TGAGCGAGTT CAAGAACCTA 
GACATCACCA AGAAGAGGTT GATCTACGAG GGCCCACTGA CGTGGCGGGT GACGAAGGAC 
AAGGCTGTGG AGGTCCACGT GCTGCTGCTG GATGACCTGC TGCTGCTGCT TCAGCGCCAG 
GACGAGCGGT TCTTGCTTAA GTCGCACAGC CGGACGCTGA CGCCCACGCC CGATGGCAAG 
ACCATGCTGC GGCCAGTGCT GCGGCTCACC TCCGCCATGA CCCGCGAGGT GGCCACCGAT 
CACAAAGCCT TCTATGTCAT TTTTACCTGG GACCAGGAGG CCCAGATATA CGAGCTGGTG 
GCGCAGACGG TGTCTGAGCG GAGGATCTGG TGTACCCTGA TCACCGAGAC CGCTGGATGC 
CTGAAGGTCC CCTCCCGCAC CTCCCGACCC AAACACCGGC CCGGCCCCAG CAGCACTCGT 
GAACCCCTGC TTAGCAGCTC CGAAAACGGC AATGGCAGCC GAGAGATGCC CCCGGCAGAT 
GGCCGGATGG AGAGACTCTT CAACTCCCTC CTGCCCTTCT GCAGACAAGG CCTCGAGGGC 
CAGCTCGCCG CCAAGGCCCT TCAGAAAGTG CTGACCCTGA AACAGCTCCT GTTGCCTTCG 
GAGGAAGACA GCGGGGCAGG GCCTCCCCTC GAAGGGGATG GGGTCCCGGG GGGCAGCCCC 
TTGAGCCCAA CGCAGCCCCA GGAAATTCGG GAGGAGCTGC TCAGCCTGGA GGAGACCATT 
AAACAGCTAG AGGAGGTGGA GGAGGAATTT TGCCGCCTGA GACTCCTCCT GTCTCAGCTT 
GGGGAGAACA CCGTCCCCCA GTCTGGCTGT ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010822008.1
CDS	210..3125
Translation	MEAEDVARGAAPGPPRPGLAPVSIIGAEDEDFEIELKTNPEEQNNQFQNLDQVKRRPAHLMALLQHVAMQFEPGPLLCCLHADMLGSLGPKEFKKAFIDFYHSFLDKHSILRVMVPPAVAFELDRTRPDLFPEDLQRQFVQEVVQSQQVAVVRQLEDFRSKKLMGMTPWEQELVQLGAWVARDRASYEARERHLAERQLTHLEEMQHTISNDEEKSAAVVNAISMYMRHLGVRTKSGDKKSGRNFFRKKMIGNRRSDEPTKTKKGLSSFLDAARWNRGEPQDFRHPKVEVDAEKPGLTERKGGQGAPSRDRNLGGPGQDTPGVSPHLLSGDSPDREPGVDALLEVGDPPPQGPASLDAPAPLESTEEAADTERKWKRLSGRLGRSESLRVSDRRRPSRGSLGAKGRGGGRSRSDVDMDPSSATAVLGPTRRATPEPGDEGEPGRSGLELEPEEPPGWRELVPQDILHSLPKSQVKRQEVISELLVTEAAHVRMLRVLHDVFYQPIADGGFYSQEELQNIFPSLDELIEVHSLFLDSLMKRRQDSGYLIKEIGDVLLARFDGAEGSWFQKISSRFCSRQSFALEQLKAKQRKEPRFCTFVQEAESRPRCRRLQLKDMIPTEMQRLTKYPLLLQSIEQNTEEPVEREKVERAAECCREILQHVNLAVRDMEDLLRLKDYQRRLDLTHLRQSNDPMLSEFKNLDITKKRLIYEGPLTWRVTKDKAVEVHVLLLDDLLLLLQRQDERFLLKSHSRTLTPTPDGKTMLRPVLRLTSAMTREVATDHKAFYVIFTWDQEAQIYELVAQTVSERRIWCTLITETAGCLKVPSRTSRPKHRPGPSSTREPLLSSSENGNGSREMPPADGRMERLFNSLLPFCRQGLEGQLAAKALQKVLTLKQLLLPSEEDSGAGPPLEGDGVPGGSPLSPTQPQEIREELLSLEETIKQLEEVEEEFCRLRLLLSQLGENTVPQSGCT

Target ORF information:

RefSeq Version	XM_010823706.2
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010823706.2

ORF Insert Sequence:

ATGGAGGCAG AAGACGTCGC CCGAGGGGCG GCCCCGGGGC CCCCCCGACC CGGCCTGGCG 
CCCGTCAGCA TCATTGGGGC AGAGGACGAG GATTTTGAGA TTGAGTTGAA GACGAACCCT 
GAGGAACAAA ACAACCAGTT CCAGAACCTG GATCAGGTGA AGCGGCGCCC GGCCCACCTC 
ATGGCCCTCC TGCAGCACGT GGCCATGCAG TTTGAGCCGG GACCCCTGCT CTGCTGCCTG 
CATGCGGACA TGCTGGGCTC CCTGGGCCCC AAGGAGTTCA AGAAAGCCTT CATCGACTTC 
TACCACAGCT TCCTGGATAA GCACTCGATT CTACGAGTGA TGGTCCCTCC TGCCGTGGCC 
TTTGAACTTG ACCGCACGCG GCCTGACCTC TTTCCTGAGG ATCTCCAGCG GCAGTTCGTG 
CAGGAGGTGG TGCAGAGCCA GCAGGTAGCC GTGGTCCGGC AGCTGGAGGA CTTCCGCTCC 
AAGAAGCTCA TGGGCATGAC ACCCTGGGAG CAGGAGCTGG TCCAGCTGGG GGCCTGGGTG 
GCGCGGGACC GTGCCAGCTA CGAGGCCCGG GAGCGGCACC TGGCTGAGCG GCAACTGACC 
CACCTGGAGG AGATGCAGCA CACCATCTCT AACGATGAGG AGAAGAGCGC CGCCGTGGTC 
AACGCCATCA GCATGTACAT GCGGCACCTT GGGGTGCGGA CCAAGAGTGG GGACAAGAAG 
TCCGGGAGGA ACTTCTTTCG GAAAAAGATG ATAGGGAACC GGCGGTCAGA TGAACCGACC 
AAGACCAAGA AAGGCCTGAG CAGCTTCCTG GATGCCGCCC GCTGGAACCG GGGAGAGCCC 
CAGGATTTTC GACACCCGAA GGTCGAAGTT GATGCTGAGA AGCCAGGCCT TACAGAACGT 
AAGGGAGGTC AGGGGGCGCC TTCCCGGGAC CGGAATCTCG GAGGCCCTGG GCAGGACACC 
CCCGGAGTTT CTCCGCACCT TCTGTCTGGG GACAGCCCTG ACCGGGAGCC AGGTGTTGAC 
GCCCTCCTGG AGGTGGGGGA CCCGCCCCCA CAGGGCCCAG CCAGCCTGGA CGCCCCAGCG 
CCGCTGGAGA GCACGGAGGA GGCTGCCGAC ACAGAAAGAA AGTGGAAGAG GCTGTCAGGG 
CGTCTGGGGC GCTCAGAGAG CCTGCGGGTG AGTGACCGCC GCCGGCCTTC CCGGGGTAGC 
CTCGGGGCTA AGGGCCGGGG TGGGGGCCGC TCCCGGAGCG ACGTGGACAT GGACCCCAGC 
TCCGCCACGG CCGTGCTTGG CCCTACCCGA CGAGCCACCC CGGAGCCTGG AGATGAGGGA 
GAGCCGGGCC GCTCAGGACT GGAGCTGGAA CCAGAGGAGC CGCCTGGCTG GCGAGAGCTT 
GTCCCCCAGG ACATTCTGCA CAGCCTGCCC AAGAGCCAGG TGAAGCGGCA GGAGGTCATC 
AGCGAGCTCC TGGTGACGGA GGCTGCCCAT GTGCGCATGC TCCGCGTGCT CCACGACGTC 
TTCTACCAGC CCATAGCGGA CGGGGGCTTC TACTCCCAGG AGGAGCTCCA GAACATCTTC 
CCCAGCCTGG ACGAGCTCAT CGAGGTGCAT TCCCTATTCC TTGATTCCCT GATGAAGCGG 
AGGCAGGACA GTGGCTACCT CATCAAGGAG ATCGGAGATG TGCTGCTGGC CCGGTTCGAT 
GGTGCCGAGG GCTCGTGGTT CCAGAAAATC TCCTCCCGCT TCTGTAGCCG CCAGTCGTTC 
GCCTTAGAGC AGCTCAAAGC CAAACAGCGC AAGGAGCCCC GGTTCTGTAC CTTCGTGCAG 
GAGGCCGAGA GCCGGCCCCG GTGCCGCCGG CTGCAGCTGA AGGACATGAT CCCCACCGAG 
ATGCAGCGTC TGACCAAGTA CCCACTGCTT CTGCAGAGCA TCGAGCAGAA CACAGAAGAG 
CCGGTGGAAC GGGAGAAAGT GGAGCGGGCA GCCGAGTGCT GCCGGGAAAT TCTGCAACAC 
GTCAACCTTG CCGTGCGGGA CATGGAGGAC CTGCTGCGGC TCAAAGATTA TCAGAGGCGT 
CTGGATTTGA CCCACCTGCG GCAGAGCAAC GACCCCATGC TGAGCGAGTT CAAGAACCTA 
GACATCACCA AGAAGAGGTT GATCTACGAG GGCCCACTGA CGTGGCGGGT GACGAAGGAC 
AAGGCTGTGG AGGTCCACGT GCTGCTGCTG GATGACCTGC TGCTGCTGCT TCAGCGCCAG 
GACGAGCGGT TCTTGCTTAA GTCGCACAGC CGGACGCTGA CGCCCACGCC CGATGGCAAG 
ACCATGCTGC GGCCAGTGCT GCGGCTCACC TCCGCCATGA CCCGCGAGGT GGCCACCGAT 
CACAAAGCCT TCTATGTCAT TTTTACCTGG GACCAGGAGG CCCAGATATA CGAGCTGGTG 
GCGCAGACGG TGTCTGAGCG GAGGATCTGG TGTACCCTGA TCACCGAGAC CGCTGGATGC 
CTGAAGGTCC CCTCCCGCAC CTCCCGACCC AAACACCGGC CCGGCCCCAG CAGCACTCGT 
GAACCCCTGC TTAGCAGCTC CGAAAACGGC AATGGCAGCC GAGAGATGCC CCCGGCAGAT 
GGCCGGATGG AGAGACTCTT CAACTCCCTC CTGCCCTTCT GCAGACAAGG CCTCGAGGGC 
CAGCTCGCCG CCAAGGCCCT TCAGAAAGTG CTGACCCTGA AACAGCTCCT GTTGCCTTCG 
GAGGAAGACA GCGGGGCAGG GCCTCCCCTC GAAGGGGATG GGGTCCCGGG GGGCAGCCCC 
TTGAGCCCAA CGCAGCCCCA GGAAATTCGG GAGGAGCTGC TCAGCCTGGA GGAGACCATT 
AAACAGCTAG AGGAGGTGGA GGAGGAATTT TGCCGCCTGA GACTCCTCCT GTCTCAGCTT 
GGGGAGAACA CCGTCCCCCA GTCTGGCTGT ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF1 ( XM_005195333.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005195390.1 Bos taurus Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa09522
Clone ID Related Accession (Same CDS sequence)	XM_010823706.2 , XM_005195333.3 , XM_005219394.3 , XM_010815120.2 , XM_005219395.3 , XM_005219394.4 , XM_005219395.4 , XM_005195332.3
Accession Version	XM_005195333.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2916bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	rho guanine nucleotide exchange factor 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646390.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005195333.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCAG AAGACGTCGC CCGAGGGGCG GCCCCGGGGC CCCCCCGACC CGGCCTGGCG 
CCCGTCAGCA TCATTGGGGC AGAGGACGAG GATTTTGAGA TTGAGTTGAA GACGAACCCT 
GAGGAACAAA ACAACCAGTT CCAGAACCTG GATCAGGTGA AGCGGCGCCC GGCCCACCTC 
ATGGCCCTCC TGCAGCACGT GGCCATGCAG TTTGAGCCGG GACCCCTGCT CTGCTGCCTG 
CATGCGGACA TGCTGGGCTC CCTGGGCCCC AAGGAGTTCA AGAAAGCCTT CATCGACTTC 
TACCACAGCT TCCTGGATAA GCACTCGATT CTACGAGTGA TGGTCCCTCC TGCCGTGGCC 
TTTGAACTTG ACCGCACGCG GCCTGACCTC TTTCCTGAGG ATCTCCAGCG GCAGTTCGTG 
CAGGAGGTGG TGCAGAGCCA GCAGGTAGCC GTGGTCCGGC AGCTGGAGGA CTTCCGCTCC 
AAGAAGCTCA TGGGCATGAC ACCCTGGGAG CAGGAGCTGG TCCAGCTGGG GGCCTGGGTG 
GCGCGGGACC GTGCCAGCTA CGAGGCCCGG GAGCGGCACC TGGCTGAGCG GCAACTGACC 
CACCTGGAGG AGATGCAGCA CACCATCTCT AACGATGAGG AGAAGAGCGC CGCCGTGGTC 
AACGCCATCA GCATGTACAT GCGGCACCTT GGGGTGCGGA CCAAGAGTGG GGACAAGAAG 
TCCGGGAGGA ACTTCTTTCG GAAAAAGATG ATAGGGAACC GGCGGTCAGA TGAACCGACC 
AAGACCAAGA AAGGCCTGAG CAGCTTCCTG GATGCCGCCC GCTGGAACCG GGGAGAGCCC 
CAGGATTTTC GACACCCGAA GGTCGAAGTT GATGCTGAGA AGCCAGGCCT TACAGAACGT 
AAGGGAGGTC AGGGGGCGCC TTCCCGGGAC CGGAATCTCG GAGGCCCTGG GCAGGACACC 
CCCGGAGTTT CTCCGCACCT TCTGTCTGGG GACAGCCCTG ACCGGGAGCC AGGTGTTGAC 
GCCCTCCTGG AGGTGGGGGA CCCGCCCCCA CAGGGCCCAG CCAGCCTGGA CGCCCCAGCG 
CCGCTGGAGA GCACGGAGGA GGCTGCCGAC ACAGAAAGAA AGTGGAAGAG GCTGTCAGGG 
CGTCTGGGGC GCTCAGAGAG CCTGCGGGTG AGTGACCGCC GCCGGCCTTC CCGGGGTAGC 
CTCGGGGCTA AGGGCCGGGG TGGGGGCCGC TCCCGGAGCG ACGTGGACAT GGACCCCAGC 
TCCGCCACGG CCGTGCTTGG CCCTACCCGA CGAGCCACCC CGGAGCCTGG AGATGAGGGA 
GAGCCGGGCC GCTCAGGACT GGAGCTGGAA CCAGAGGAGC CGCCTGGCTG GCGAGAGCTT 
GTCCCCCAGG ACATTCTGCA CAGCCTGCCC AAGAGCCAGG TGAAGCGGCA GGAGGTCATC 
AGCGAGCTCC TGGTGACGGA GGCTGCCCAT GTGCGCATGC TCCGCGTGCT CCACGACGTC 
TTCTACCAGC CCATAGCGGA CGGGGGCTTC TACTCCCAGG AGGAGCTCCA GAACATCTTC 
CCCAGCCTGG ACGAGCTCAT CGAGGTGCAT TCCCTATTCC TTGATTCCCT GATGAAGCGG 
AGGCAGGACA GTGGCTACCT CATCAAGGAG ATCGGAGATG TGCTGCTGGC CCGGTTCGAT 
GGTGCCGAGG GCTCGTGGTT CCAGAAAATC TCCTCCCGCT TCTGTAGCCG CCAGTCGTTC 
GCCTTAGAGC AGCTCAAAGC CAAACAGCGC AAGGAGCCCC GGTTCTGTAC CTTCGTGCAG 
GAGGCCGAGA GCCGGCCCCG GTGCCGCCGG CTGCAGCTGA AGGACATGAT CCCCACCGAG 
ATGCAGCGTC TGACCAAGTA CCCACTGCTT CTGCAGAGCA TCGAGCAGAA CACAGAAGAG 
CCGGTGGAAC GGGAGAAAGT GGAGCGGGCA GCCGAGTGCT GCCGGGAAAT TCTGCAACAC 
GTCAACCTTG CCGTGCGGGA CATGGAGGAC CTGCTGCGGC TCAAAGATTA TCAGAGGCGT 
CTGGATTTGA CCCACCTGCG GCAGAGCAAC GACCCCATGC TGAGCGAGTT CAAGAACCTA 
GACATCACCA AGAAGAGGTT GATCTACGAG GGCCCACTGA CGTGGCGGGT GACGAAGGAC 
AAGGCTGTGG AGGTCCACGT GCTGCTGCTG GATGACCTGC TGCTGCTGCT TCAGCGCCAG 
GACGAGCGGT TCTTGCTTAA GTCGCACAGC CGGACGCTGA CGCCCACGCC CGATGGCAAG 
ACCATGCTGC GGCCAGTGCT GCGGCTCACC TCCGCCATGA CCCGCGAGGT GGCCACCGAT 
CACAAAGCCT TCTATGTCAT TTTTACCTGG GACCAGGAGG CCCAGATATA CGAGCTGGTG 
GCGCAGACGG TGTCTGAGCG GAGGATCTGG TGTACCCTGA TCACCGAGAC CGCTGGATGC 
CTGAAGGTCC CCTCCCGCAC CTCCCGACCC AAACACCGGC CCGGCCCCAG CAGCACTCGT 
GAACCCCTGC TTAGCAGCTC CGAAAACGGC AATGGCAGCC GAGAGATGCC CCCGGCAGAT 
GGCCGGATGG AGAGACTCTT CAACTCCCTC CTGCCCTTCT GCAGACAAGG CCTCGAGGGC 
CAGCTCGCCG CCAAGGCCCT TCAGAAAGTG CTGACCCTGA AACAGCTCCT GTTGCCTTCG 
GAGGAAGACA GCGGGGCAGG GCCTCCCCTC GAAGGGGATG GGGTCCCGGG GGGCAGCCCC 
TTGAGCCCAA CGCAGCCCCA GGAAATTCGG GAGGAGCTGC TCAGCCTGGA GGAGACCATT 
AAACAGCTAG AGGAGGTGGA GGAGGAATTT TGCCGCCTGA GACTCCTCCT GTCTCAGCTT 
GGGGAGAACA CCGTCCCCCA GTCTGGCTGT ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005195390.1
CDS	307..3222
Translation	MEAEDVARGAAPGPPRPGLAPVSIIGAEDEDFEIELKTNPEEQNNQFQNLDQVKRRPAHLMALLQHVAMQFEPGPLLCCLHADMLGSLGPKEFKKAFIDFYHSFLDKHSILRVMVPPAVAFELDRTRPDLFPEDLQRQFVQEVVQSQQVAVVRQLEDFRSKKLMGMTPWEQELVQLGAWVARDRASYEARERHLAERQLTHLEEMQHTISNDEEKSAAVVNAISMYMRHLGVRTKSGDKKSGRNFFRKKMIGNRRSDEPTKTKKGLSSFLDAARWNRGEPQDFRHPKVEVDAEKPGLTERKGGQGAPSRDRNLGGPGQDTPGVSPHLLSGDSPDREPGVDALLEVGDPPPQGPASLDAPAPLESTEEAADTERKWKRLSGRLGRSESLRVSDRRRPSRGSLGAKGRGGGRSRSDVDMDPSSATAVLGPTRRATPEPGDEGEPGRSGLELEPEEPPGWRELVPQDILHSLPKSQVKRQEVISELLVTEAAHVRMLRVLHDVFYQPIADGGFYSQEELQNIFPSLDELIEVHSLFLDSLMKRRQDSGYLIKEIGDVLLARFDGAEGSWFQKISSRFCSRQSFALEQLKAKQRKEPRFCTFVQEAESRPRCRRLQLKDMIPTEMQRLTKYPLLLQSIEQNTEEPVEREKVERAAECCREILQHVNLAVRDMEDLLRLKDYQRRLDLTHLRQSNDPMLSEFKNLDITKKRLIYEGPLTWRVTKDKAVEVHVLLLDDLLLLLQRQDERFLLKSHSRTLTPTPDGKTMLRPVLRLTSAMTREVATDHKAFYVIFTWDQEAQIYELVAQTVSERRIWCTLITETAGCLKVPSRTSRPKHRPGPSSTREPLLSSSENGNGSREMPPADGRMERLFNSLLPFCRQGLEGQLAAKALQKVLTLKQLLLPSEEDSGAGPPLEGDGVPGGSPLSPTQPQEIREELLSLEETIKQLEEVEEEFCRLRLLLSQLGENTVPQSGCT

Target ORF information:

RefSeq Version	XM_005195333.3
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005195333.3

ORF Insert Sequence:

ATGGAGGCAG AAGACGTCGC CCGAGGGGCG GCCCCGGGGC CCCCCCGACC CGGCCTGGCG 
CCCGTCAGCA TCATTGGGGC AGAGGACGAG GATTTTGAGA TTGAGTTGAA GACGAACCCT 
GAGGAACAAA ACAACCAGTT CCAGAACCTG GATCAGGTGA AGCGGCGCCC GGCCCACCTC 
ATGGCCCTCC TGCAGCACGT GGCCATGCAG TTTGAGCCGG GACCCCTGCT CTGCTGCCTG 
CATGCGGACA TGCTGGGCTC CCTGGGCCCC AAGGAGTTCA AGAAAGCCTT CATCGACTTC 
TACCACAGCT TCCTGGATAA GCACTCGATT CTACGAGTGA TGGTCCCTCC TGCCGTGGCC 
TTTGAACTTG ACCGCACGCG GCCTGACCTC TTTCCTGAGG ATCTCCAGCG GCAGTTCGTG 
CAGGAGGTGG TGCAGAGCCA GCAGGTAGCC GTGGTCCGGC AGCTGGAGGA CTTCCGCTCC 
AAGAAGCTCA TGGGCATGAC ACCCTGGGAG CAGGAGCTGG TCCAGCTGGG GGCCTGGGTG 
GCGCGGGACC GTGCCAGCTA CGAGGCCCGG GAGCGGCACC TGGCTGAGCG GCAACTGACC 
CACCTGGAGG AGATGCAGCA CACCATCTCT AACGATGAGG AGAAGAGCGC CGCCGTGGTC 
AACGCCATCA GCATGTACAT GCGGCACCTT GGGGTGCGGA CCAAGAGTGG GGACAAGAAG 
TCCGGGAGGA ACTTCTTTCG GAAAAAGATG ATAGGGAACC GGCGGTCAGA TGAACCGACC 
AAGACCAAGA AAGGCCTGAG CAGCTTCCTG GATGCCGCCC GCTGGAACCG GGGAGAGCCC 
CAGGATTTTC GACACCCGAA GGTCGAAGTT GATGCTGAGA AGCCAGGCCT TACAGAACGT 
AAGGGAGGTC AGGGGGCGCC TTCCCGGGAC CGGAATCTCG GAGGCCCTGG GCAGGACACC 
CCCGGAGTTT CTCCGCACCT TCTGTCTGGG GACAGCCCTG ACCGGGAGCC AGGTGTTGAC 
GCCCTCCTGG AGGTGGGGGA CCCGCCCCCA CAGGGCCCAG CCAGCCTGGA CGCCCCAGCG 
CCGCTGGAGA GCACGGAGGA GGCTGCCGAC ACAGAAAGAA AGTGGAAGAG GCTGTCAGGG 
CGTCTGGGGC GCTCAGAGAG CCTGCGGGTG AGTGACCGCC GCCGGCCTTC CCGGGGTAGC 
CTCGGGGCTA AGGGCCGGGG TGGGGGCCGC TCCCGGAGCG ACGTGGACAT GGACCCCAGC 
TCCGCCACGG CCGTGCTTGG CCCTACCCGA CGAGCCACCC CGGAGCCTGG AGATGAGGGA 
GAGCCGGGCC GCTCAGGACT GGAGCTGGAA CCAGAGGAGC CGCCTGGCTG GCGAGAGCTT 
GTCCCCCAGG ACATTCTGCA CAGCCTGCCC AAGAGCCAGG TGAAGCGGCA GGAGGTCATC 
AGCGAGCTCC TGGTGACGGA GGCTGCCCAT GTGCGCATGC TCCGCGTGCT CCACGACGTC 
TTCTACCAGC CCATAGCGGA CGGGGGCTTC TACTCCCAGG AGGAGCTCCA GAACATCTTC 
CCCAGCCTGG ACGAGCTCAT CGAGGTGCAT TCCCTATTCC TTGATTCCCT GATGAAGCGG 
AGGCAGGACA GTGGCTACCT CATCAAGGAG ATCGGAGATG TGCTGCTGGC CCGGTTCGAT 
GGTGCCGAGG GCTCGTGGTT CCAGAAAATC TCCTCCCGCT TCTGTAGCCG CCAGTCGTTC 
GCCTTAGAGC AGCTCAAAGC CAAACAGCGC AAGGAGCCCC GGTTCTGTAC CTTCGTGCAG 
GAGGCCGAGA GCCGGCCCCG GTGCCGCCGG CTGCAGCTGA AGGACATGAT CCCCACCGAG 
ATGCAGCGTC TGACCAAGTA CCCACTGCTT CTGCAGAGCA TCGAGCAGAA CACAGAAGAG 
CCGGTGGAAC GGGAGAAAGT GGAGCGGGCA GCCGAGTGCT GCCGGGAAAT TCTGCAACAC 
GTCAACCTTG CCGTGCGGGA CATGGAGGAC CTGCTGCGGC TCAAAGATTA TCAGAGGCGT 
CTGGATTTGA CCCACCTGCG GCAGAGCAAC GACCCCATGC TGAGCGAGTT CAAGAACCTA 
GACATCACCA AGAAGAGGTT GATCTACGAG GGCCCACTGA CGTGGCGGGT GACGAAGGAC 
AAGGCTGTGG AGGTCCACGT GCTGCTGCTG GATGACCTGC TGCTGCTGCT TCAGCGCCAG 
GACGAGCGGT TCTTGCTTAA GTCGCACAGC CGGACGCTGA CGCCCACGCC CGATGGCAAG 
ACCATGCTGC GGCCAGTGCT GCGGCTCACC TCCGCCATGA CCCGCGAGGT GGCCACCGAT 
CACAAAGCCT TCTATGTCAT TTTTACCTGG GACCAGGAGG CCCAGATATA CGAGCTGGTG 
GCGCAGACGG TGTCTGAGCG GAGGATCTGG TGTACCCTGA TCACCGAGAC CGCTGGATGC 
CTGAAGGTCC CCTCCCGCAC CTCCCGACCC AAACACCGGC CCGGCCCCAG CAGCACTCGT 
GAACCCCTGC TTAGCAGCTC CGAAAACGGC AATGGCAGCC GAGAGATGCC CCCGGCAGAT 
GGCCGGATGG AGAGACTCTT CAACTCCCTC CTGCCCTTCT GCAGACAAGG CCTCGAGGGC 
CAGCTCGCCG CCAAGGCCCT TCAGAAAGTG CTGACCCTGA AACAGCTCCT GTTGCCTTCG 
GAGGAAGACA GCGGGGCAGG GCCTCCCCTC GAAGGGGATG GGGTCCCGGG GGGCAGCCCC 
TTGAGCCCAA CGCAGCCCCA GGAAATTCGG GAGGAGCTGC TCAGCCTGGA GGAGACCATT 
AAACAGCTAG AGGAGGTGGA GGAGGAATTT TGCCGCCTGA GACTCCTCCT GTCTCAGCTT 
GGGGAGAACA CCGTCCCCCA GTCTGGCTGT ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF1 ( XM_005219394.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005219451.1 Bos taurus Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa09522
Clone ID Related Accession (Same CDS sequence)	XM_010823706.2 , XM_005195333.3 , XM_005219394.3 , XM_010815120.2 , XM_005219395.3 , XM_005219394.4 , XM_005219395.4 , XM_005195332.3
Accession Version	XM_005219394.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2916bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	rho guanine nucleotide exchange factor 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104466.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005219394.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCAG AAGACGTCGC CCGAGGGGCG GCCCCGGGGC CCCCCCGACC CGGCCTGGCG 
CCCGTCAGCA TCATTGGGGC AGAGGACGAG GATTTTGAGA TTGAGTTGAA GACGAACCCT 
GAGGAACAAA ACAACCAGTT CCAGAACCTG GATCAGGTGA AGCGGCGCCC GGCCCACCTC 
ATGGCCCTCC TGCAGCACGT GGCCATGCAG TTTGAGCCGG GACCCCTGCT CTGCTGCCTG 
CATGCGGACA TGCTGGGCTC CCTGGGCCCC AAGGAGTTCA AGAAAGCCTT CATCGACTTC 
TACCACAGCT TCCTGGATAA GCACTCGATT CTACGAGTGA TGGTCCCTCC TGCCGTGGCC 
TTTGAACTTG ACCGCACGCG GCCTGACCTC TTTCCTGAGG ATCTCCAGCG GCAGTTCGTG 
CAGGAGGTGG TGCAGAGCCA GCAGGTAGCC GTGGTCCGGC AGCTGGAGGA CTTCCGCTCC 
AAGAAGCTCA TGGGCATGAC ACCCTGGGAG CAGGAGCTGG TCCAGCTGGG GGCCTGGGTG 
GCGCGGGACC GTGCCAGCTA CGAGGCCCGG GAGCGGCACC TGGCTGAGCG GCAACTGACC 
CACCTGGAGG AGATGCAGCA CACCATCTCT AACGATGAGG AGAAGAGCGC CGCCGTGGTC 
AACGCCATCA GCATGTACAT GCGGCACCTT GGGGTGCGGA CCAAGAGTGG GGACAAGAAG 
TCCGGGAGGA ACTTCTTTCG GAAAAAGATG ATAGGGAACC GGCGGTCAGA TGAACCGACC 
AAGACCAAGA AAGGCCTGAG CAGCTTCCTG GATGCCGCCC GCTGGAACCG GGGAGAGCCC 
CAGGATTTTC GACACCCGAA GGTCGAAGTT GATGCTGAGA AGCCAGGCCT TACAGAACGT 
AAGGGAGGTC AGGGGGCGCC TTCCCGGGAC CGGAATCTCG GAGGCCCTGG GCAGGACACC 
CCCGGAGTTT CTCCGCACCT TCTGTCTGGG GACAGCCCTG ACCGGGAGCC AGGTGTTGAC 
GCCCTCCTGG AGGTGGGGGA CCCGCCCCCA CAGGGCCCAG CCAGCCTGGA CGCCCCAGCG 
CCGCTGGAGA GCACGGAGGA GGCTGCCGAC ACAGAAAGAA AGTGGAAGAG GCTGTCAGGG 
CGTCTGGGGC GCTCAGAGAG CCTGCGGGTG AGTGACCGCC GCCGGCCTTC CCGGGGTAGC 
CTCGGGGCTA AGGGCCGGGG TGGGGGCCGC TCCCGGAGCG ACGTGGACAT GGACCCCAGC 
TCCGCCACGG CCGTGCTTGG CCCTACCCGA CGAGCCACCC CGGAGCCTGG AGATGAGGGA 
GAGCCGGGCC GCTCAGGACT GGAGCTGGAA CCAGAGGAGC CGCCTGGCTG GCGAGAGCTT 
GTCCCCCAGG ACATTCTGCA CAGCCTGCCC AAGAGCCAGG TGAAGCGGCA GGAGGTCATC 
AGCGAGCTCC TGGTGACGGA GGCTGCCCAT GTGCGCATGC TCCGCGTGCT CCACGACGTC 
TTCTACCAGC CCATAGCGGA CGGGGGCTTC TACTCCCAGG AGGAGCTCCA GAACATCTTC 
CCCAGCCTGG ACGAGCTCAT CGAGGTGCAT TCCCTATTCC TTGATTCCCT GATGAAGCGG 
AGGCAGGACA GTGGCTACCT CATCAAGGAG ATCGGAGATG TGCTGCTGGC CCGGTTCGAT 
GGTGCCGAGG GCTCGTGGTT CCAGAAAATC TCCTCCCGCT TCTGTAGCCG CCAGTCGTTC 
GCCTTAGAGC AGCTCAAAGC CAAACAGCGC AAGGAGCCCC GGTTCTGTAC CTTCGTGCAG 
GAGGCCGAGA GCCGGCCCCG GTGCCGCCGG CTGCAGCTGA AGGACATGAT CCCCACCGAG 
ATGCAGCGTC TGACCAAGTA CCCACTGCTT CTGCAGAGCA TCGAGCAGAA CACAGAAGAG 
CCGGTGGAAC GGGAGAAAGT GGAGCGGGCA GCCGAGTGCT GCCGGGAAAT TCTGCAACAC 
GTCAACCTTG CCGTGCGGGA CATGGAGGAC CTGCTGCGGC TCAAAGATTA TCAGAGGCGT 
CTGGATTTGA CCCACCTGCG GCAGAGCAAC GACCCCATGC TGAGCGAGTT CAAGAACCTA 
GACATCACCA AGAAGAGGTT GATCTACGAG GGCCCACTGA CGTGGCGGGT GACGAAGGAC 
AAGGCTGTGG AGGTCCACGT GCTGCTGCTG GATGACCTGC TGCTGCTGCT TCAGCGCCAG 
GACGAGCGGT TCTTGCTTAA GTCGCACAGC CGGACGCTGA CGCCCACGCC CGATGGCAAG 
ACCATGCTGC GGCCAGTGCT GCGGCTCACC TCCGCCATGA CCCGCGAGGT GGCCACCGAT 
CACAAAGCCT TCTATGTCAT TTTTACCTGG GACCAGGAGG CCCAGATATA CGAGCTGGTG 
GCGCAGACGG TGTCTGAGCG GAGGATCTGG TGTACCCTGA TCACCGAGAC CGCTGGATGC 
CTGAAGGTCC CCTCCCGCAC CTCCCGACCC AAACACCGGC CCGGCCCCAG CAGCACTCGT 
GAACCCCTGC TTAGCAGCTC CGAAAACGGC AATGGCAGCC GAGAGATGCC CCCGGCAGAT 
GGCCGGATGG AGAGACTCTT CAACTCCCTC CTGCCCTTCT GCAGACAAGG CCTCGAGGGC 
CAGCTCGCCG CCAAGGCCCT TCAGAAAGTG CTGACCCTGA AACAGCTCCT GTTGCCTTCG 
GAGGAAGACA GCGGGGCAGG GCCTCCCCTC GAAGGGGATG GGGTCCCGGG GGGCAGCCCC 
TTGAGCCCAA CGCAGCCCCA GGAAATTCGG GAGGAGCTGC TCAGCCTGGA GGAGACCATT 
AAACAGCTAG AGGAGGTGGA GGAGGAATTT TGCCGCCTGA GACTCCTCCT GTCTCAGCTT 
GGGGAGAACA CCGTCCCCCA GTCTGGCTGT ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005219451.1
CDS	470..3385
Translation	MEAEDVARGAAPGPPRPGLAPVSIIGAEDEDFEIELKTNPEEQNNQFQNLDQVKRRPAHLMALLQHVAMQFEPGPLLCCLHADMLGSLGPKEFKKAFIDFYHSFLDKHSILRVMVPPAVAFELDRTRPDLFPEDLQRQFVQEVVQSQQVAVVRQLEDFRSKKLMGMTPWEQELVQLGAWVARDRASYEARERHLAERQLTHLEEMQHTISNDEEKSAAVVNAISMYMRHLGVRTKSGDKKSGRNFFRKKMIGNRRSDEPTKTKKGLSSFLDAARWNRGEPQDFRHPKVEVDAEKPGLTERKGGQGAPSRDRNLGGPGQDTPGVSPHLLSGDSPDREPGVDALLEVGDPPPQGPASLDAPAPLESTEEAADTERKWKRLSGRLGRSESLRVSDRRRPSRGSLGAKGRGGGRSRSDVDMDPSSATAVLGPTRRATPEPGDEGEPGRSGLELEPEEPPGWRELVPQDILHSLPKSQVKRQEVISELLVTEAAHVRMLRVLHDVFYQPIADGGFYSQEELQNIFPSLDELIEVHSLFLDSLMKRRQDSGYLIKEIGDVLLARFDGAEGSWFQKISSRFCSRQSFALEQLKAKQRKEPRFCTFVQEAESRPRCRRLQLKDMIPTEMQRLTKYPLLLQSIEQNTEEPVEREKVERAAECCREILQHVNLAVRDMEDLLRLKDYQRRLDLTHLRQSNDPMLSEFKNLDITKKRLIYEGPLTWRVTKDKAVEVHVLLLDDLLLLLQRQDERFLLKSHSRTLTPTPDGKTMLRPVLRLTSAMTREVATDHKAFYVIFTWDQEAQIYELVAQTVSERRIWCTLITETAGCLKVPSRTSRPKHRPGPSSTREPLLSSSENGNGSREMPPADGRMERLFNSLLPFCRQGLEGQLAAKALQKVLTLKQLLLPSEEDSGAGPPLEGDGVPGGSPLSPTQPQEIREELLSLEETIKQLEEVEEEFCRLRLLLSQLGENTVPQSGCT

Target ORF information:

RefSeq Version	XM_005219394.3
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005219394.3

ORF Insert Sequence:

ATGGAGGCAG AAGACGTCGC CCGAGGGGCG GCCCCGGGGC CCCCCCGACC CGGCCTGGCG 
CCCGTCAGCA TCATTGGGGC AGAGGACGAG GATTTTGAGA TTGAGTTGAA GACGAACCCT 
GAGGAACAAA ACAACCAGTT CCAGAACCTG GATCAGGTGA AGCGGCGCCC GGCCCACCTC 
ATGGCCCTCC TGCAGCACGT GGCCATGCAG TTTGAGCCGG GACCCCTGCT CTGCTGCCTG 
CATGCGGACA TGCTGGGCTC CCTGGGCCCC AAGGAGTTCA AGAAAGCCTT CATCGACTTC 
TACCACAGCT TCCTGGATAA GCACTCGATT CTACGAGTGA TGGTCCCTCC TGCCGTGGCC 
TTTGAACTTG ACCGCACGCG GCCTGACCTC TTTCCTGAGG ATCTCCAGCG GCAGTTCGTG 
CAGGAGGTGG TGCAGAGCCA GCAGGTAGCC GTGGTCCGGC AGCTGGAGGA CTTCCGCTCC 
AAGAAGCTCA TGGGCATGAC ACCCTGGGAG CAGGAGCTGG TCCAGCTGGG GGCCTGGGTG 
GCGCGGGACC GTGCCAGCTA CGAGGCCCGG GAGCGGCACC TGGCTGAGCG GCAACTGACC 
CACCTGGAGG AGATGCAGCA CACCATCTCT AACGATGAGG AGAAGAGCGC CGCCGTGGTC 
AACGCCATCA GCATGTACAT GCGGCACCTT GGGGTGCGGA CCAAGAGTGG GGACAAGAAG 
TCCGGGAGGA ACTTCTTTCG GAAAAAGATG ATAGGGAACC GGCGGTCAGA TGAACCGACC 
AAGACCAAGA AAGGCCTGAG CAGCTTCCTG GATGCCGCCC GCTGGAACCG GGGAGAGCCC 
CAGGATTTTC GACACCCGAA GGTCGAAGTT GATGCTGAGA AGCCAGGCCT TACAGAACGT 
AAGGGAGGTC AGGGGGCGCC TTCCCGGGAC CGGAATCTCG GAGGCCCTGG GCAGGACACC 
CCCGGAGTTT CTCCGCACCT TCTGTCTGGG GACAGCCCTG ACCGGGAGCC AGGTGTTGAC 
GCCCTCCTGG AGGTGGGGGA CCCGCCCCCA CAGGGCCCAG CCAGCCTGGA CGCCCCAGCG 
CCGCTGGAGA GCACGGAGGA GGCTGCCGAC ACAGAAAGAA AGTGGAAGAG GCTGTCAGGG 
CGTCTGGGGC GCTCAGAGAG CCTGCGGGTG AGTGACCGCC GCCGGCCTTC CCGGGGTAGC 
CTCGGGGCTA AGGGCCGGGG TGGGGGCCGC TCCCGGAGCG ACGTGGACAT GGACCCCAGC 
TCCGCCACGG CCGTGCTTGG CCCTACCCGA CGAGCCACCC CGGAGCCTGG AGATGAGGGA 
GAGCCGGGCC GCTCAGGACT GGAGCTGGAA CCAGAGGAGC CGCCTGGCTG GCGAGAGCTT 
GTCCCCCAGG ACATTCTGCA CAGCCTGCCC AAGAGCCAGG TGAAGCGGCA GGAGGTCATC 
AGCGAGCTCC TGGTGACGGA GGCTGCCCAT GTGCGCATGC TCCGCGTGCT CCACGACGTC 
TTCTACCAGC CCATAGCGGA CGGGGGCTTC TACTCCCAGG AGGAGCTCCA GAACATCTTC 
CCCAGCCTGG ACGAGCTCAT CGAGGTGCAT TCCCTATTCC TTGATTCCCT GATGAAGCGG 
AGGCAGGACA GTGGCTACCT CATCAAGGAG ATCGGAGATG TGCTGCTGGC CCGGTTCGAT 
GGTGCCGAGG GCTCGTGGTT CCAGAAAATC TCCTCCCGCT TCTGTAGCCG CCAGTCGTTC 
GCCTTAGAGC AGCTCAAAGC CAAACAGCGC AAGGAGCCCC GGTTCTGTAC CTTCGTGCAG 
GAGGCCGAGA GCCGGCCCCG GTGCCGCCGG CTGCAGCTGA AGGACATGAT CCCCACCGAG 
ATGCAGCGTC TGACCAAGTA CCCACTGCTT CTGCAGAGCA TCGAGCAGAA CACAGAAGAG 
CCGGTGGAAC GGGAGAAAGT GGAGCGGGCA GCCGAGTGCT GCCGGGAAAT TCTGCAACAC 
GTCAACCTTG CCGTGCGGGA CATGGAGGAC CTGCTGCGGC TCAAAGATTA TCAGAGGCGT 
CTGGATTTGA CCCACCTGCG GCAGAGCAAC GACCCCATGC TGAGCGAGTT CAAGAACCTA 
GACATCACCA AGAAGAGGTT GATCTACGAG GGCCCACTGA CGTGGCGGGT GACGAAGGAC 
AAGGCTGTGG AGGTCCACGT GCTGCTGCTG GATGACCTGC TGCTGCTGCT TCAGCGCCAG 
GACGAGCGGT TCTTGCTTAA GTCGCACAGC CGGACGCTGA CGCCCACGCC CGATGGCAAG 
ACCATGCTGC GGCCAGTGCT GCGGCTCACC TCCGCCATGA CCCGCGAGGT GGCCACCGAT 
CACAAAGCCT TCTATGTCAT TTTTACCTGG GACCAGGAGG CCCAGATATA CGAGCTGGTG 
GCGCAGACGG TGTCTGAGCG GAGGATCTGG TGTACCCTGA TCACCGAGAC CGCTGGATGC 
CTGAAGGTCC CCTCCCGCAC CTCCCGACCC AAACACCGGC CCGGCCCCAG CAGCACTCGT 
GAACCCCTGC TTAGCAGCTC CGAAAACGGC AATGGCAGCC GAGAGATGCC CCCGGCAGAT 
GGCCGGATGG AGAGACTCTT CAACTCCCTC CTGCCCTTCT GCAGACAAGG CCTCGAGGGC 
CAGCTCGCCG CCAAGGCCCT TCAGAAAGTG CTGACCCTGA AACAGCTCCT GTTGCCTTCG 
GAGGAAGACA GCGGGGCAGG GCCTCCCCTC GAAGGGGATG GGGTCCCGGG GGGCAGCCCC 
TTGAGCCCAA CGCAGCCCCA GGAAATTCGG GAGGAGCTGC TCAGCCTGGA GGAGACCATT 
AAACAGCTAG AGGAGGTGGA GGAGGAATTT TGCCGCCTGA GACTCCTCCT GTCTCAGCTT 
GGGGAGAACA CCGTCCCCCA GTCTGGCTGT ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF1 ( XM_010815120.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010813422.1 Bos taurus Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa09522
Clone ID Related Accession (Same CDS sequence)	XM_010823706.2 , XM_005195333.3 , XM_005219394.3 , XM_010815120.2 , XM_005219395.3 , XM_005219394.4 , XM_005219395.4 , XM_005195332.3
Accession Version	XM_010815120.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2916bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	rho guanine nucleotide exchange factor 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104466.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010815120.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCAG AAGACGTCGC CCGAGGGGCG GCCCCGGGGC CCCCCCGACC CGGCCTGGCG 
CCCGTCAGCA TCATTGGGGC AGAGGACGAG GATTTTGAGA TTGAGTTGAA GACGAACCCT 
GAGGAACAAA ACAACCAGTT CCAGAACCTG GATCAGGTGA AGCGGCGCCC GGCCCACCTC 
ATGGCCCTCC TGCAGCACGT GGCCATGCAG TTTGAGCCGG GACCCCTGCT CTGCTGCCTG 
CATGCGGACA TGCTGGGCTC CCTGGGCCCC AAGGAGTTCA AGAAAGCCTT CATCGACTTC 
TACCACAGCT TCCTGGATAA GCACTCGATT CTACGAGTGA TGGTCCCTCC TGCCGTGGCC 
TTTGAACTTG ACCGCACGCG GCCTGACCTC TTTCCTGAGG ATCTCCAGCG GCAGTTCGTG 
CAGGAGGTGG TGCAGAGCCA GCAGGTAGCC GTGGTCCGGC AGCTGGAGGA CTTCCGCTCC 
AAGAAGCTCA TGGGCATGAC ACCCTGGGAG CAGGAGCTGG TCCAGCTGGG GGCCTGGGTG 
GCGCGGGACC GTGCCAGCTA CGAGGCCCGG GAGCGGCACC TGGCTGAGCG GCAACTGACC 
CACCTGGAGG AGATGCAGCA CACCATCTCT AACGATGAGG AGAAGAGCGC CGCCGTGGTC 
AACGCCATCA GCATGTACAT GCGGCACCTT GGGGTGCGGA CCAAGAGTGG GGACAAGAAG 
TCCGGGAGGA ACTTCTTTCG GAAAAAGATG ATAGGGAACC GGCGGTCAGA TGAACCGACC 
AAGACCAAGA AAGGCCTGAG CAGCTTCCTG GATGCCGCCC GCTGGAACCG GGGAGAGCCC 
CAGGATTTTC GACACCCGAA GGTCGAAGTT GATGCTGAGA AGCCAGGCCT TACAGAACGT 
AAGGGAGGTC AGGGGGCGCC TTCCCGGGAC CGGAATCTCG GAGGCCCTGG GCAGGACACC 
CCCGGAGTTT CTCCGCACCT TCTGTCTGGG GACAGCCCTG ACCGGGAGCC AGGTGTTGAC 
GCCCTCCTGG AGGTGGGGGA CCCGCCCCCA CAGGGCCCAG CCAGCCTGGA CGCCCCAGCG 
CCGCTGGAGA GCACGGAGGA GGCTGCCGAC ACAGAAAGAA AGTGGAAGAG GCTGTCAGGG 
CGTCTGGGGC GCTCAGAGAG CCTGCGGGTG AGTGACCGCC GCCGGCCTTC CCGGGGTAGC 
CTCGGGGCTA AGGGCCGGGG TGGGGGCCGC TCCCGGAGCG ACGTGGACAT GGACCCCAGC 
TCCGCCACGG CCGTGCTTGG CCCTACCCGA CGAGCCACCC CGGAGCCTGG AGATGAGGGA 
GAGCCGGGCC GCTCAGGACT GGAGCTGGAA CCAGAGGAGC CGCCTGGCTG GCGAGAGCTT 
GTCCCCCAGG ACATTCTGCA CAGCCTGCCC AAGAGCCAGG TGAAGCGGCA GGAGGTCATC 
AGCGAGCTCC TGGTGACGGA GGCTGCCCAT GTGCGCATGC TCCGCGTGCT CCACGACGTC 
TTCTACCAGC CCATAGCGGA CGGGGGCTTC TACTCCCAGG AGGAGCTCCA GAACATCTTC 
CCCAGCCTGG ACGAGCTCAT CGAGGTGCAT TCCCTATTCC TTGATTCCCT GATGAAGCGG 
AGGCAGGACA GTGGCTACCT CATCAAGGAG ATCGGAGATG TGCTGCTGGC CCGGTTCGAT 
GGTGCCGAGG GCTCGTGGTT CCAGAAAATC TCCTCCCGCT TCTGTAGCCG CCAGTCGTTC 
GCCTTAGAGC AGCTCAAAGC CAAACAGCGC AAGGAGCCCC GGTTCTGTAC CTTCGTGCAG 
GAGGCCGAGA GCCGGCCCCG GTGCCGCCGG CTGCAGCTGA AGGACATGAT CCCCACCGAG 
ATGCAGCGTC TGACCAAGTA CCCACTGCTT CTGCAGAGCA TCGAGCAGAA CACAGAAGAG 
CCGGTGGAAC GGGAGAAAGT GGAGCGGGCA GCCGAGTGCT GCCGGGAAAT TCTGCAACAC 
GTCAACCTTG CCGTGCGGGA CATGGAGGAC CTGCTGCGGC TCAAAGATTA TCAGAGGCGT 
CTGGATTTGA CCCACCTGCG GCAGAGCAAC GACCCCATGC TGAGCGAGTT CAAGAACCTA 
GACATCACCA AGAAGAGGTT GATCTACGAG GGCCCACTGA CGTGGCGGGT GACGAAGGAC 
AAGGCTGTGG AGGTCCACGT GCTGCTGCTG GATGACCTGC TGCTGCTGCT TCAGCGCCAG 
GACGAGCGGT TCTTGCTTAA GTCGCACAGC CGGACGCTGA CGCCCACGCC CGATGGCAAG 
ACCATGCTGC GGCCAGTGCT GCGGCTCACC TCCGCCATGA CCCGCGAGGT GGCCACCGAT 
CACAAAGCCT TCTATGTCAT TTTTACCTGG GACCAGGAGG CCCAGATATA CGAGCTGGTG 
GCGCAGACGG TGTCTGAGCG GAGGATCTGG TGTACCCTGA TCACCGAGAC CGCTGGATGC 
CTGAAGGTCC CCTCCCGCAC CTCCCGACCC AAACACCGGC CCGGCCCCAG CAGCACTCGT 
GAACCCCTGC TTAGCAGCTC CGAAAACGGC AATGGCAGCC GAGAGATGCC CCCGGCAGAT 
GGCCGGATGG AGAGACTCTT CAACTCCCTC CTGCCCTTCT GCAGACAAGG CCTCGAGGGC 
CAGCTCGCCG CCAAGGCCCT TCAGAAAGTG CTGACCCTGA AACAGCTCCT GTTGCCTTCG 
GAGGAAGACA GCGGGGCAGG GCCTCCCCTC GAAGGGGATG GGGTCCCGGG GGGCAGCCCC 
TTGAGCCCAA CGCAGCCCCA GGAAATTCGG GAGGAGCTGC TCAGCCTGGA GGAGACCATT 
AAACAGCTAG AGGAGGTGGA GGAGGAATTT TGCCGCCTGA GACTCCTCCT GTCTCAGCTT 
GGGGAGAACA CCGTCCCCCA GTCTGGCTGT ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010813422.1
CDS	210..3125
Translation	MEAEDVARGAAPGPPRPGLAPVSIIGAEDEDFEIELKTNPEEQNNQFQNLDQVKRRPAHLMALLQHVAMQFEPGPLLCCLHADMLGSLGPKEFKKAFIDFYHSFLDKHSILRVMVPPAVAFELDRTRPDLFPEDLQRQFVQEVVQSQQVAVVRQLEDFRSKKLMGMTPWEQELVQLGAWVARDRASYEARERHLAERQLTHLEEMQHTISNDEEKSAAVVNAISMYMRHLGVRTKSGDKKSGRNFFRKKMIGNRRSDEPTKTKKGLSSFLDAARWNRGEPQDFRHPKVEVDAEKPGLTERKGGQGAPSRDRNLGGPGQDTPGVSPHLLSGDSPDREPGVDALLEVGDPPPQGPASLDAPAPLESTEEAADTERKWKRLSGRLGRSESLRVSDRRRPSRGSLGAKGRGGGRSRSDVDMDPSSATAVLGPTRRATPEPGDEGEPGRSGLELEPEEPPGWRELVPQDILHSLPKSQVKRQEVISELLVTEAAHVRMLRVLHDVFYQPIADGGFYSQEELQNIFPSLDELIEVHSLFLDSLMKRRQDSGYLIKEIGDVLLARFDGAEGSWFQKISSRFCSRQSFALEQLKAKQRKEPRFCTFVQEAESRPRCRRLQLKDMIPTEMQRLTKYPLLLQSIEQNTEEPVEREKVERAAECCREILQHVNLAVRDMEDLLRLKDYQRRLDLTHLRQSNDPMLSEFKNLDITKKRLIYEGPLTWRVTKDKAVEVHVLLLDDLLLLLQRQDERFLLKSHSRTLTPTPDGKTMLRPVLRLTSAMTREVATDHKAFYVIFTWDQEAQIYELVAQTVSERRIWCTLITETAGCLKVPSRTSRPKHRPGPSSTREPLLSSSENGNGSREMPPADGRMERLFNSLLPFCRQGLEGQLAAKALQKVLTLKQLLLPSEEDSGAGPPLEGDGVPGGSPLSPTQPQEIREELLSLEETIKQLEEVEEEFCRLRLLLSQLGENTVPQSGCT

Target ORF information:

RefSeq Version	XM_010815120.2
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010815120.2

ORF Insert Sequence:

ATGGAGGCAG AAGACGTCGC CCGAGGGGCG GCCCCGGGGC CCCCCCGACC CGGCCTGGCG 
CCCGTCAGCA TCATTGGGGC AGAGGACGAG GATTTTGAGA TTGAGTTGAA GACGAACCCT 
GAGGAACAAA ACAACCAGTT CCAGAACCTG GATCAGGTGA AGCGGCGCCC GGCCCACCTC 
ATGGCCCTCC TGCAGCACGT GGCCATGCAG TTTGAGCCGG GACCCCTGCT CTGCTGCCTG 
CATGCGGACA TGCTGGGCTC CCTGGGCCCC AAGGAGTTCA AGAAAGCCTT CATCGACTTC 
TACCACAGCT TCCTGGATAA GCACTCGATT CTACGAGTGA TGGTCCCTCC TGCCGTGGCC 
TTTGAACTTG ACCGCACGCG GCCTGACCTC TTTCCTGAGG ATCTCCAGCG GCAGTTCGTG 
CAGGAGGTGG TGCAGAGCCA GCAGGTAGCC GTGGTCCGGC AGCTGGAGGA CTTCCGCTCC 
AAGAAGCTCA TGGGCATGAC ACCCTGGGAG CAGGAGCTGG TCCAGCTGGG GGCCTGGGTG 
GCGCGGGACC GTGCCAGCTA CGAGGCCCGG GAGCGGCACC TGGCTGAGCG GCAACTGACC 
CACCTGGAGG AGATGCAGCA CACCATCTCT AACGATGAGG AGAAGAGCGC CGCCGTGGTC 
AACGCCATCA GCATGTACAT GCGGCACCTT GGGGTGCGGA CCAAGAGTGG GGACAAGAAG 
TCCGGGAGGA ACTTCTTTCG GAAAAAGATG ATAGGGAACC GGCGGTCAGA TGAACCGACC 
AAGACCAAGA AAGGCCTGAG CAGCTTCCTG GATGCCGCCC GCTGGAACCG GGGAGAGCCC 
CAGGATTTTC GACACCCGAA GGTCGAAGTT GATGCTGAGA AGCCAGGCCT TACAGAACGT 
AAGGGAGGTC AGGGGGCGCC TTCCCGGGAC CGGAATCTCG GAGGCCCTGG GCAGGACACC 
CCCGGAGTTT CTCCGCACCT TCTGTCTGGG GACAGCCCTG ACCGGGAGCC AGGTGTTGAC 
GCCCTCCTGG AGGTGGGGGA CCCGCCCCCA CAGGGCCCAG CCAGCCTGGA CGCCCCAGCG 
CCGCTGGAGA GCACGGAGGA GGCTGCCGAC ACAGAAAGAA AGTGGAAGAG GCTGTCAGGG 
CGTCTGGGGC GCTCAGAGAG CCTGCGGGTG AGTGACCGCC GCCGGCCTTC CCGGGGTAGC 
CTCGGGGCTA AGGGCCGGGG TGGGGGCCGC TCCCGGAGCG ACGTGGACAT GGACCCCAGC 
TCCGCCACGG CCGTGCTTGG CCCTACCCGA CGAGCCACCC CGGAGCCTGG AGATGAGGGA 
GAGCCGGGCC GCTCAGGACT GGAGCTGGAA CCAGAGGAGC CGCCTGGCTG GCGAGAGCTT 
GTCCCCCAGG ACATTCTGCA CAGCCTGCCC AAGAGCCAGG TGAAGCGGCA GGAGGTCATC 
AGCGAGCTCC TGGTGACGGA GGCTGCCCAT GTGCGCATGC TCCGCGTGCT CCACGACGTC 
TTCTACCAGC CCATAGCGGA CGGGGGCTTC TACTCCCAGG AGGAGCTCCA GAACATCTTC 
CCCAGCCTGG ACGAGCTCAT CGAGGTGCAT TCCCTATTCC TTGATTCCCT GATGAAGCGG 
AGGCAGGACA GTGGCTACCT CATCAAGGAG ATCGGAGATG TGCTGCTGGC CCGGTTCGAT 
GGTGCCGAGG GCTCGTGGTT CCAGAAAATC TCCTCCCGCT TCTGTAGCCG CCAGTCGTTC 
GCCTTAGAGC AGCTCAAAGC CAAACAGCGC AAGGAGCCCC GGTTCTGTAC CTTCGTGCAG 
GAGGCCGAGA GCCGGCCCCG GTGCCGCCGG CTGCAGCTGA AGGACATGAT CCCCACCGAG 
ATGCAGCGTC TGACCAAGTA CCCACTGCTT CTGCAGAGCA TCGAGCAGAA CACAGAAGAG 
CCGGTGGAAC GGGAGAAAGT GGAGCGGGCA GCCGAGTGCT GCCGGGAAAT TCTGCAACAC 
GTCAACCTTG CCGTGCGGGA CATGGAGGAC CTGCTGCGGC TCAAAGATTA TCAGAGGCGT 
CTGGATTTGA CCCACCTGCG GCAGAGCAAC GACCCCATGC TGAGCGAGTT CAAGAACCTA 
GACATCACCA AGAAGAGGTT GATCTACGAG GGCCCACTGA CGTGGCGGGT GACGAAGGAC 
AAGGCTGTGG AGGTCCACGT GCTGCTGCTG GATGACCTGC TGCTGCTGCT TCAGCGCCAG 
GACGAGCGGT TCTTGCTTAA GTCGCACAGC CGGACGCTGA CGCCCACGCC CGATGGCAAG 
ACCATGCTGC GGCCAGTGCT GCGGCTCACC TCCGCCATGA CCCGCGAGGT GGCCACCGAT 
CACAAAGCCT TCTATGTCAT TTTTACCTGG GACCAGGAGG CCCAGATATA CGAGCTGGTG 
GCGCAGACGG TGTCTGAGCG GAGGATCTGG TGTACCCTGA TCACCGAGAC CGCTGGATGC 
CTGAAGGTCC CCTCCCGCAC CTCCCGACCC AAACACCGGC CCGGCCCCAG CAGCACTCGT 
GAACCCCTGC TTAGCAGCTC CGAAAACGGC AATGGCAGCC GAGAGATGCC CCCGGCAGAT 
GGCCGGATGG AGAGACTCTT CAACTCCCTC CTGCCCTTCT GCAGACAAGG CCTCGAGGGC 
CAGCTCGCCG CCAAGGCCCT TCAGAAAGTG CTGACCCTGA AACAGCTCCT GTTGCCTTCG 
GAGGAAGACA GCGGGGCAGG GCCTCCCCTC GAAGGGGATG GGGTCCCGGG GGGCAGCCCC 
TTGAGCCCAA CGCAGCCCCA GGAAATTCGG GAGGAGCTGC TCAGCCTGGA GGAGACCATT 
AAACAGCTAG AGGAGGTGGA GGAGGAATTT TGCCGCCTGA GACTCCTCCT GTCTCAGCTT 
GGGGAGAACA CCGTCCCCCA GTCTGGCTGT ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF1 ( XM_005219395.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005219452.1 Bos taurus Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa09522
Clone ID Related Accession (Same CDS sequence)	XM_010823706.2 , XM_005195333.3 , XM_005219394.3 , XM_010815120.2 , XM_005219395.3 , XM_005219394.4 , XM_005219395.4 , XM_005195332.3
Accession Version	XM_005219395.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2916bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	rho guanine nucleotide exchange factor 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104466.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005219395.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCAG AAGACGTCGC CCGAGGGGCG GCCCCGGGGC CCCCCCGACC CGGCCTGGCG 
CCCGTCAGCA TCATTGGGGC AGAGGACGAG GATTTTGAGA TTGAGTTGAA GACGAACCCT 
GAGGAACAAA ACAACCAGTT CCAGAACCTG GATCAGGTGA AGCGGCGCCC GGCCCACCTC 
ATGGCCCTCC TGCAGCACGT GGCCATGCAG TTTGAGCCGG GACCCCTGCT CTGCTGCCTG 
CATGCGGACA TGCTGGGCTC CCTGGGCCCC AAGGAGTTCA AGAAAGCCTT CATCGACTTC 
TACCACAGCT TCCTGGATAA GCACTCGATT CTACGAGTGA TGGTCCCTCC TGCCGTGGCC 
TTTGAACTTG ACCGCACGCG GCCTGACCTC TTTCCTGAGG ATCTCCAGCG GCAGTTCGTG 
CAGGAGGTGG TGCAGAGCCA GCAGGTAGCC GTGGTCCGGC AGCTGGAGGA CTTCCGCTCC 
AAGAAGCTCA TGGGCATGAC ACCCTGGGAG CAGGAGCTGG TCCAGCTGGG GGCCTGGGTG 
GCGCGGGACC GTGCCAGCTA CGAGGCCCGG GAGCGGCACC TGGCTGAGCG GCAACTGACC 
CACCTGGAGG AGATGCAGCA CACCATCTCT AACGATGAGG AGAAGAGCGC CGCCGTGGTC 
AACGCCATCA GCATGTACAT GCGGCACCTT GGGGTGCGGA CCAAGAGTGG GGACAAGAAG 
TCCGGGAGGA ACTTCTTTCG GAAAAAGATG ATAGGGAACC GGCGGTCAGA TGAACCGACC 
AAGACCAAGA AAGGCCTGAG CAGCTTCCTG GATGCCGCCC GCTGGAACCG GGGAGAGCCC 
CAGGATTTTC GACACCCGAA GGTCGAAGTT GATGCTGAGA AGCCAGGCCT TACAGAACGT 
AAGGGAGGTC AGGGGGCGCC TTCCCGGGAC CGGAATCTCG GAGGCCCTGG GCAGGACACC 
CCCGGAGTTT CTCCGCACCT TCTGTCTGGG GACAGCCCTG ACCGGGAGCC AGGTGTTGAC 
GCCCTCCTGG AGGTGGGGGA CCCGCCCCCA CAGGGCCCAG CCAGCCTGGA CGCCCCAGCG 
CCGCTGGAGA GCACGGAGGA GGCTGCCGAC ACAGAAAGAA AGTGGAAGAG GCTGTCAGGG 
CGTCTGGGGC GCTCAGAGAG CCTGCGGGTG AGTGACCGCC GCCGGCCTTC CCGGGGTAGC 
CTCGGGGCTA AGGGCCGGGG TGGGGGCCGC TCCCGGAGCG ACGTGGACAT GGACCCCAGC 
TCCGCCACGG CCGTGCTTGG CCCTACCCGA CGAGCCACCC CGGAGCCTGG AGATGAGGGA 
GAGCCGGGCC GCTCAGGACT GGAGCTGGAA CCAGAGGAGC CGCCTGGCTG GCGAGAGCTT 
GTCCCCCAGG ACATTCTGCA CAGCCTGCCC AAGAGCCAGG TGAAGCGGCA GGAGGTCATC 
AGCGAGCTCC TGGTGACGGA GGCTGCCCAT GTGCGCATGC TCCGCGTGCT CCACGACGTC 
TTCTACCAGC CCATAGCGGA CGGGGGCTTC TACTCCCAGG AGGAGCTCCA GAACATCTTC 
CCCAGCCTGG ACGAGCTCAT CGAGGTGCAT TCCCTATTCC TTGATTCCCT GATGAAGCGG 
AGGCAGGACA GTGGCTACCT CATCAAGGAG ATCGGAGATG TGCTGCTGGC CCGGTTCGAT 
GGTGCCGAGG GCTCGTGGTT CCAGAAAATC TCCTCCCGCT TCTGTAGCCG CCAGTCGTTC 
GCCTTAGAGC AGCTCAAAGC CAAACAGCGC AAGGAGCCCC GGTTCTGTAC CTTCGTGCAG 
GAGGCCGAGA GCCGGCCCCG GTGCCGCCGG CTGCAGCTGA AGGACATGAT CCCCACCGAG 
ATGCAGCGTC TGACCAAGTA CCCACTGCTT CTGCAGAGCA TCGAGCAGAA CACAGAAGAG 
CCGGTGGAAC GGGAGAAAGT GGAGCGGGCA GCCGAGTGCT GCCGGGAAAT TCTGCAACAC 
GTCAACCTTG CCGTGCGGGA CATGGAGGAC CTGCTGCGGC TCAAAGATTA TCAGAGGCGT 
CTGGATTTGA CCCACCTGCG GCAGAGCAAC GACCCCATGC TGAGCGAGTT CAAGAACCTA 
GACATCACCA AGAAGAGGTT GATCTACGAG GGCCCACTGA CGTGGCGGGT GACGAAGGAC 
AAGGCTGTGG AGGTCCACGT GCTGCTGCTG GATGACCTGC TGCTGCTGCT TCAGCGCCAG 
GACGAGCGGT TCTTGCTTAA GTCGCACAGC CGGACGCTGA CGCCCACGCC CGATGGCAAG 
ACCATGCTGC GGCCAGTGCT GCGGCTCACC TCCGCCATGA CCCGCGAGGT GGCCACCGAT 
CACAAAGCCT TCTATGTCAT TTTTACCTGG GACCAGGAGG CCCAGATATA CGAGCTGGTG 
GCGCAGACGG TGTCTGAGCG GAGGATCTGG TGTACCCTGA TCACCGAGAC CGCTGGATGC 
CTGAAGGTCC CCTCCCGCAC CTCCCGACCC AAACACCGGC CCGGCCCCAG CAGCACTCGT 
GAACCCCTGC TTAGCAGCTC CGAAAACGGC AATGGCAGCC GAGAGATGCC CCCGGCAGAT 
GGCCGGATGG AGAGACTCTT CAACTCCCTC CTGCCCTTCT GCAGACAAGG CCTCGAGGGC 
CAGCTCGCCG CCAAGGCCCT TCAGAAAGTG CTGACCCTGA AACAGCTCCT GTTGCCTTCG 
GAGGAAGACA GCGGGGCAGG GCCTCCCCTC GAAGGGGATG GGGTCCCGGG GGGCAGCCCC 
TTGAGCCCAA CGCAGCCCCA GGAAATTCGG GAGGAGCTGC TCAGCCTGGA GGAGACCATT 
AAACAGCTAG AGGAGGTGGA GGAGGAATTT TGCCGCCTGA GACTCCTCCT GTCTCAGCTT 
GGGGAGAACA CCGTCCCCCA GTCTGGCTGT ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005219452.1
CDS	307..3222
Translation	MEAEDVARGAAPGPPRPGLAPVSIIGAEDEDFEIELKTNPEEQNNQFQNLDQVKRRPAHLMALLQHVAMQFEPGPLLCCLHADMLGSLGPKEFKKAFIDFYHSFLDKHSILRVMVPPAVAFELDRTRPDLFPEDLQRQFVQEVVQSQQVAVVRQLEDFRSKKLMGMTPWEQELVQLGAWVARDRASYEARERHLAERQLTHLEEMQHTISNDEEKSAAVVNAISMYMRHLGVRTKSGDKKSGRNFFRKKMIGNRRSDEPTKTKKGLSSFLDAARWNRGEPQDFRHPKVEVDAEKPGLTERKGGQGAPSRDRNLGGPGQDTPGVSPHLLSGDSPDREPGVDALLEVGDPPPQGPASLDAPAPLESTEEAADTERKWKRLSGRLGRSESLRVSDRRRPSRGSLGAKGRGGGRSRSDVDMDPSSATAVLGPTRRATPEPGDEGEPGRSGLELEPEEPPGWRELVPQDILHSLPKSQVKRQEVISELLVTEAAHVRMLRVLHDVFYQPIADGGFYSQEELQNIFPSLDELIEVHSLFLDSLMKRRQDSGYLIKEIGDVLLARFDGAEGSWFQKISSRFCSRQSFALEQLKAKQRKEPRFCTFVQEAESRPRCRRLQLKDMIPTEMQRLTKYPLLLQSIEQNTEEPVEREKVERAAECCREILQHVNLAVRDMEDLLRLKDYQRRLDLTHLRQSNDPMLSEFKNLDITKKRLIYEGPLTWRVTKDKAVEVHVLLLDDLLLLLQRQDERFLLKSHSRTLTPTPDGKTMLRPVLRLTSAMTREVATDHKAFYVIFTWDQEAQIYELVAQTVSERRIWCTLITETAGCLKVPSRTSRPKHRPGPSSTREPLLSSSENGNGSREMPPADGRMERLFNSLLPFCRQGLEGQLAAKALQKVLTLKQLLLPSEEDSGAGPPLEGDGVPGGSPLSPTQPQEIREELLSLEETIKQLEEVEEEFCRLRLLLSQLGENTVPQSGCT

Target ORF information:

RefSeq Version	XM_005219395.3
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005219395.3

ORF Insert Sequence:

ATGGAGGCAG AAGACGTCGC CCGAGGGGCG GCCCCGGGGC CCCCCCGACC CGGCCTGGCG 
CCCGTCAGCA TCATTGGGGC AGAGGACGAG GATTTTGAGA TTGAGTTGAA GACGAACCCT 
GAGGAACAAA ACAACCAGTT CCAGAACCTG GATCAGGTGA AGCGGCGCCC GGCCCACCTC 
ATGGCCCTCC TGCAGCACGT GGCCATGCAG TTTGAGCCGG GACCCCTGCT CTGCTGCCTG 
CATGCGGACA TGCTGGGCTC CCTGGGCCCC AAGGAGTTCA AGAAAGCCTT CATCGACTTC 
TACCACAGCT TCCTGGATAA GCACTCGATT CTACGAGTGA TGGTCCCTCC TGCCGTGGCC 
TTTGAACTTG ACCGCACGCG GCCTGACCTC TTTCCTGAGG ATCTCCAGCG GCAGTTCGTG 
CAGGAGGTGG TGCAGAGCCA GCAGGTAGCC GTGGTCCGGC AGCTGGAGGA CTTCCGCTCC 
AAGAAGCTCA TGGGCATGAC ACCCTGGGAG CAGGAGCTGG TCCAGCTGGG GGCCTGGGTG 
GCGCGGGACC GTGCCAGCTA CGAGGCCCGG GAGCGGCACC TGGCTGAGCG GCAACTGACC 
CACCTGGAGG AGATGCAGCA CACCATCTCT AACGATGAGG AGAAGAGCGC CGCCGTGGTC 
AACGCCATCA GCATGTACAT GCGGCACCTT GGGGTGCGGA CCAAGAGTGG GGACAAGAAG 
TCCGGGAGGA ACTTCTTTCG GAAAAAGATG ATAGGGAACC GGCGGTCAGA TGAACCGACC 
AAGACCAAGA AAGGCCTGAG CAGCTTCCTG GATGCCGCCC GCTGGAACCG GGGAGAGCCC 
CAGGATTTTC GACACCCGAA GGTCGAAGTT GATGCTGAGA AGCCAGGCCT TACAGAACGT 
AAGGGAGGTC AGGGGGCGCC TTCCCGGGAC CGGAATCTCG GAGGCCCTGG GCAGGACACC 
CCCGGAGTTT CTCCGCACCT TCTGTCTGGG GACAGCCCTG ACCGGGAGCC AGGTGTTGAC 
GCCCTCCTGG AGGTGGGGGA CCCGCCCCCA CAGGGCCCAG CCAGCCTGGA CGCCCCAGCG 
CCGCTGGAGA GCACGGAGGA GGCTGCCGAC ACAGAAAGAA AGTGGAAGAG GCTGTCAGGG 
CGTCTGGGGC GCTCAGAGAG CCTGCGGGTG AGTGACCGCC GCCGGCCTTC CCGGGGTAGC 
CTCGGGGCTA AGGGCCGGGG TGGGGGCCGC TCCCGGAGCG ACGTGGACAT GGACCCCAGC 
TCCGCCACGG CCGTGCTTGG CCCTACCCGA CGAGCCACCC CGGAGCCTGG AGATGAGGGA 
GAGCCGGGCC GCTCAGGACT GGAGCTGGAA CCAGAGGAGC CGCCTGGCTG GCGAGAGCTT 
GTCCCCCAGG ACATTCTGCA CAGCCTGCCC AAGAGCCAGG TGAAGCGGCA GGAGGTCATC 
AGCGAGCTCC TGGTGACGGA GGCTGCCCAT GTGCGCATGC TCCGCGTGCT CCACGACGTC 
TTCTACCAGC CCATAGCGGA CGGGGGCTTC TACTCCCAGG AGGAGCTCCA GAACATCTTC 
CCCAGCCTGG ACGAGCTCAT CGAGGTGCAT TCCCTATTCC TTGATTCCCT GATGAAGCGG 
AGGCAGGACA GTGGCTACCT CATCAAGGAG ATCGGAGATG TGCTGCTGGC CCGGTTCGAT 
GGTGCCGAGG GCTCGTGGTT CCAGAAAATC TCCTCCCGCT TCTGTAGCCG CCAGTCGTTC 
GCCTTAGAGC AGCTCAAAGC CAAACAGCGC AAGGAGCCCC GGTTCTGTAC CTTCGTGCAG 
GAGGCCGAGA GCCGGCCCCG GTGCCGCCGG CTGCAGCTGA AGGACATGAT CCCCACCGAG 
ATGCAGCGTC TGACCAAGTA CCCACTGCTT CTGCAGAGCA TCGAGCAGAA CACAGAAGAG 
CCGGTGGAAC GGGAGAAAGT GGAGCGGGCA GCCGAGTGCT GCCGGGAAAT TCTGCAACAC 
GTCAACCTTG CCGTGCGGGA CATGGAGGAC CTGCTGCGGC TCAAAGATTA TCAGAGGCGT 
CTGGATTTGA CCCACCTGCG GCAGAGCAAC GACCCCATGC TGAGCGAGTT CAAGAACCTA 
GACATCACCA AGAAGAGGTT GATCTACGAG GGCCCACTGA CGTGGCGGGT GACGAAGGAC 
AAGGCTGTGG AGGTCCACGT GCTGCTGCTG GATGACCTGC TGCTGCTGCT TCAGCGCCAG 
GACGAGCGGT TCTTGCTTAA GTCGCACAGC CGGACGCTGA CGCCCACGCC CGATGGCAAG 
ACCATGCTGC GGCCAGTGCT GCGGCTCACC TCCGCCATGA CCCGCGAGGT GGCCACCGAT 
CACAAAGCCT TCTATGTCAT TTTTACCTGG GACCAGGAGG CCCAGATATA CGAGCTGGTG 
GCGCAGACGG TGTCTGAGCG GAGGATCTGG TGTACCCTGA TCACCGAGAC CGCTGGATGC 
CTGAAGGTCC CCTCCCGCAC CTCCCGACCC AAACACCGGC CCGGCCCCAG CAGCACTCGT 
GAACCCCTGC TTAGCAGCTC CGAAAACGGC AATGGCAGCC GAGAGATGCC CCCGGCAGAT 
GGCCGGATGG AGAGACTCTT CAACTCCCTC CTGCCCTTCT GCAGACAAGG CCTCGAGGGC 
CAGCTCGCCG CCAAGGCCCT TCAGAAAGTG CTGACCCTGA AACAGCTCCT GTTGCCTTCG 
GAGGAAGACA GCGGGGCAGG GCCTCCCCTC GAAGGGGATG GGGTCCCGGG GGGCAGCCCC 
TTGAGCCCAA CGCAGCCCCA GGAAATTCGG GAGGAGCTGC TCAGCCTGGA GGAGACCATT 
AAACAGCTAG AGGAGGTGGA GGAGGAATTT TGCCGCCTGA GACTCCTCCT GTCTCAGCTT 
GGGGAGAACA CCGTCCCCCA GTCTGGCTGT ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF1 ( XM_005219394.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005219451.1 Bos taurus Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OBa09522
Clone ID Related Accession (Same CDS sequence)	XM_010823706.2 , XM_005195333.3 , XM_005219394.3 , XM_010815120.2 , XM_005219395.3 , XM_005219394.4 , XM_005219395.4 , XM_005195332.3
Accession Version	XM_005219394.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2916bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rho guanine nucleotide exchange factor 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005219394.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCAG AAGACGTCGC CCGAGGGGCG GCCCCGGGGC CCCCCCGACC CGGCCTGGCG 
CCCGTCAGCA TCATTGGGGC AGAGGACGAG GATTTTGAGA TTGAGTTGAA GACGAACCCT 
GAGGAACAAA ACAACCAGTT CCAGAACCTG GATCAGGTGA AGCGGCGCCC GGCCCACCTC 
ATGGCCCTCC TGCAGCACGT GGCCATGCAG TTTGAGCCGG GACCCCTGCT CTGCTGCCTG 
CATGCGGACA TGCTGGGCTC CCTGGGCCCC AAGGAGTTCA AGAAAGCCTT CATCGACTTC 
TACCACAGCT TCCTGGATAA GCACTCGATT CTACGAGTGA TGGTCCCTCC TGCCGTGGCC 
TTTGAACTTG ACCGCACGCG GCCTGACCTC TTTCCTGAGG ATCTCCAGCG GCAGTTCGTG 
CAGGAGGTGG TGCAGAGCCA GCAGGTAGCC GTGGTCCGGC AGCTGGAGGA CTTCCGCTCC 
AAGAAGCTCA TGGGCATGAC ACCCTGGGAG CAGGAGCTGG TCCAGCTGGG GGCCTGGGTG 
GCGCGGGACC GTGCCAGCTA CGAGGCCCGG GAGCGGCACC TGGCTGAGCG GCAACTGACC 
CACCTGGAGG AGATGCAGCA CACCATCTCT AACGATGAGG AGAAGAGCGC CGCCGTGGTC 
AACGCCATCA GCATGTACAT GCGGCACCTT GGGGTGCGGA CCAAGAGTGG GGACAAGAAG 
TCCGGGAGGA ACTTCTTTCG GAAAAAGATG ATAGGGAACC GGCGGTCAGA TGAACCGACC 
AAGACCAAGA AAGGCCTGAG CAGCTTCCTG GATGCCGCCC GCTGGAACCG GGGAGAGCCC 
CAGGATTTTC GACACCCGAA GGTCGAAGTT GATGCTGAGA AGCCAGGCCT TACAGAACGT 
AAGGGAGGTC AGGGGGCGCC TTCCCGGGAC CGGAATCTCG GAGGCCCTGG GCAGGACACC 
CCCGGAGTTT CTCCGCACCT TCTGTCTGGG GACAGCCCTG ACCGGGAGCC AGGTGTTGAC 
GCCCTCCTGG AGGTGGGGGA CCCGCCCCCA CAGGGCCCAG CCAGCCTGGA CGCCCCAGCG 
CCGCTGGAGA GCACGGAGGA GGCTGCCGAC ACAGAAAGAA AGTGGAAGAG GCTGTCAGGG 
CGTCTGGGGC GCTCAGAGAG CCTGCGGGTG AGTGACCGCC GCCGGCCTTC CCGGGGTAGC 
CTCGGGGCTA AGGGCCGGGG TGGGGGCCGC TCCCGGAGCG ACGTGGACAT GGACCCCAGC 
TCCGCCACGG CCGTGCTTGG CCCTACCCGA CGAGCCACCC CGGAGCCTGG AGATGAGGGA 
GAGCCGGGCC GCTCAGGACT GGAGCTGGAA CCAGAGGAGC CGCCTGGCTG GCGAGAGCTT 
GTCCCCCAGG ACATTCTGCA CAGCCTGCCC AAGAGCCAGG TGAAGCGGCA GGAGGTCATC 
AGCGAGCTCC TGGTGACGGA GGCTGCCCAT GTGCGCATGC TCCGCGTGCT CCACGACGTC 
TTCTACCAGC CCATAGCGGA CGGGGGCTTC TACTCCCAGG AGGAGCTCCA GAACATCTTC 
CCCAGCCTGG ACGAGCTCAT CGAGGTGCAT TCCCTATTCC TTGATTCCCT GATGAAGCGG 
AGGCAGGACA GTGGCTACCT CATCAAGGAG ATCGGAGATG TGCTGCTGGC CCGGTTCGAT 
GGTGCCGAGG GCTCGTGGTT CCAGAAAATC TCCTCCCGCT TCTGTAGCCG CCAGTCGTTC 
GCCTTAGAGC AGCTCAAAGC CAAACAGCGC AAGGAGCCCC GGTTCTGTAC CTTCGTGCAG 
GAGGCCGAGA GCCGGCCCCG GTGCCGCCGG CTGCAGCTGA AGGACATGAT CCCCACCGAG 
ATGCAGCGTC TGACCAAGTA CCCACTGCTT CTGCAGAGCA TCGAGCAGAA CACAGAAGAG 
CCGGTGGAAC GGGAGAAAGT GGAGCGGGCA GCCGAGTGCT GCCGGGAAAT TCTGCAACAC 
GTCAACCTTG CCGTGCGGGA CATGGAGGAC CTGCTGCGGC TCAAAGATTA TCAGAGGCGT 
CTGGATTTGA CCCACCTGCG GCAGAGCAAC GACCCCATGC TGAGCGAGTT CAAGAACCTA 
GACATCACCA AGAAGAGGTT GATCTACGAG GGCCCACTGA CGTGGCGGGT GACGAAGGAC 
AAGGCTGTGG AGGTCCACGT GCTGCTGCTG GATGACCTGC TGCTGCTGCT TCAGCGCCAG 
GACGAGCGGT TCTTGCTTAA GTCGCACAGC CGGACGCTGA CGCCCACGCC CGATGGCAAG 
ACCATGCTGC GGCCAGTGCT GCGGCTCACC TCCGCCATGA CCCGCGAGGT GGCCACCGAT 
CACAAAGCCT TCTATGTCAT TTTTACCTGG GACCAGGAGG CCCAGATATA CGAGCTGGTG 
GCGCAGACGG TGTCTGAGCG GAGGATCTGG TGTACCCTGA TCACCGAGAC CGCTGGATGC 
CTGAAGGTCC CCTCCCGCAC CTCCCGACCC AAACACCGGC CCGGCCCCAG CAGCACTCGT 
GAACCCCTGC TTAGCAGCTC CGAAAACGGC AATGGCAGCC GAGAGATGCC CCCGGCAGAT 
GGCCGGATGG AGAGACTCTT CAACTCCCTC CTGCCCTTCT GCAGACAAGG CCTCGAGGGC 
CAGCTCGCCG CCAAGGCCCT TCAGAAAGTG CTGACCCTGA AACAGCTCCT GTTGCCTTCG 
GAGGAAGACA GCGGGGCAGG GCCTCCCCTC GAAGGGGATG GGGTCCCGGG GGGCAGCCCC 
TTGAGCCCAA CGCAGCCCCA GGAAATTCGG GAGGAGCTGC TCAGCCTGGA GGAGACCATT 
AAACAGCTAG AGGAGGTGGA GGAGGAATTT TGCCGCCTGA GACTCCTCCT GTCTCAGCTT 
GGGGAGAACA CCGTCCCCCA GTCTGGCTGT ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005219451.1
CDS	195..3110
Translation	MEAEDVARGAAPGPPRPGLAPVSIIGAEDEDFEIELKTNPEEQNNQFQNLDQVKRRPAHLMALLQHVAMQFEPGPLLCCLHADMLGSLGPKEFKKAFIDFYHSFLDKHSILRVMVPPAVAFELDRTRPDLFPEDLQRQFVQEVVQSQQVAVVRQLEDFRSKKLMGMTPWEQELVQLGAWVARDRASYEARERHLAERQLTHLEEMQHTISNDEEKSAAVVNAISMYMRHLGVRTKSGDKKSGRNFFRKKMIGNRRSDEPTKTKKGLSSFLDAARWNRGEPQDFRHPKVEVDAEKPGLTERKGGQGAPSRDRNLGGPGQDTPGVSPHLLSGDSPDREPGVDALLEVGDPPPQGPASLDAPAPLESTEEAADTERKWKRLSGRLGRSESLRVSDRRRPSRGSLGAKGRGGGRSRSDVDMDPSSATAVLGPTRRATPEPGDEGEPGRSGLELEPEEPPGWRELVPQDILHSLPKSQVKRQEVISELLVTEAAHVRMLRVLHDVFYQPIADGGFYSQEELQNIFPSLDELIEVHSLFLDSLMKRRQDSGYLIKEIGDVLLARFDGAEGSWFQKISSRFCSRQSFALEQLKAKQRKEPRFCTFVQEAESRPRCRRLQLKDMIPTEMQRLTKYPLLLQSIEQNTEEPVEREKVERAAECCREILQHVNLAVRDMEDLLRLKDYQRRLDLTHLRQSNDPMLSEFKNLDITKKRLIYEGPLTWRVTKDKAVEVHVLLLDDLLLLLQRQDERFLLKSHSRTLTPTPDGKTMLRPVLRLTSAMTREVATDHKAFYVIFTWDQEAQIYELVAQTVSERRIWCTLITETAGCLKVPSRTSRPKHRPGPSSTREPLLSSSENGNGSREMPPADGRMERLFNSLLPFCRQGLEGQLAAKALQKVLTLKQLLLPSEEDSGAGPPLEGDGVPGGSPLSPTQPQEIREELLSLEETIKQLEEVEEEFCRLRLLLSQLGENTVPQSGCT

Target ORF information:

RefSeq Version	XM_005219394.4
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005219394.4

ORF Insert Sequence:

ATGGAGGCAG AAGACGTCGC CCGAGGGGCG GCCCCGGGGC CCCCCCGACC CGGCCTGGCG 
CCCGTCAGCA TCATTGGGGC AGAGGACGAG GATTTTGAGA TTGAGTTGAA GACGAACCCT 
GAGGAACAAA ACAACCAGTT CCAGAACCTG GATCAGGTGA AGCGGCGCCC GGCCCACCTC 
ATGGCCCTCC TGCAGCACGT GGCCATGCAG TTTGAGCCGG GACCCCTGCT CTGCTGCCTG 
CATGCGGACA TGCTGGGCTC CCTGGGCCCC AAGGAGTTCA AGAAAGCCTT CATCGACTTC 
TACCACAGCT TCCTGGATAA GCACTCGATT CTACGAGTGA TGGTCCCTCC TGCCGTGGCC 
TTTGAACTTG ACCGCACGCG GCCTGACCTC TTTCCTGAGG ATCTCCAGCG GCAGTTCGTG 
CAGGAGGTGG TGCAGAGCCA GCAGGTAGCC GTGGTCCGGC AGCTGGAGGA CTTCCGCTCC 
AAGAAGCTCA TGGGCATGAC ACCCTGGGAG CAGGAGCTGG TCCAGCTGGG GGCCTGGGTG 
GCGCGGGACC GTGCCAGCTA CGAGGCCCGG GAGCGGCACC TGGCTGAGCG GCAACTGACC 
CACCTGGAGG AGATGCAGCA CACCATCTCT AACGATGAGG AGAAGAGCGC CGCCGTGGTC 
AACGCCATCA GCATGTACAT GCGGCACCTT GGGGTGCGGA CCAAGAGTGG GGACAAGAAG 
TCCGGGAGGA ACTTCTTTCG GAAAAAGATG ATAGGGAACC GGCGGTCAGA TGAACCGACC 
AAGACCAAGA AAGGCCTGAG CAGCTTCCTG GATGCCGCCC GCTGGAACCG GGGAGAGCCC 
CAGGATTTTC GACACCCGAA GGTCGAAGTT GATGCTGAGA AGCCAGGCCT TACAGAACGT 
AAGGGAGGTC AGGGGGCGCC TTCCCGGGAC CGGAATCTCG GAGGCCCTGG GCAGGACACC 
CCCGGAGTTT CTCCGCACCT TCTGTCTGGG GACAGCCCTG ACCGGGAGCC AGGTGTTGAC 
GCCCTCCTGG AGGTGGGGGA CCCGCCCCCA CAGGGCCCAG CCAGCCTGGA CGCCCCAGCG 
CCGCTGGAGA GCACGGAGGA GGCTGCCGAC ACAGAAAGAA AGTGGAAGAG GCTGTCAGGG 
CGTCTGGGGC GCTCAGAGAG CCTGCGGGTG AGTGACCGCC GCCGGCCTTC CCGGGGTAGC 
CTCGGGGCTA AGGGCCGGGG TGGGGGCCGC TCCCGGAGCG ACGTGGACAT GGACCCCAGC 
TCCGCCACGG CCGTGCTTGG CCCTACCCGA CGAGCCACCC CGGAGCCTGG AGATGAGGGA 
GAGCCGGGCC GCTCAGGACT GGAGCTGGAA CCAGAGGAGC CGCCTGGCTG GCGAGAGCTT 
GTCCCCCAGG ACATTCTGCA CAGCCTGCCC AAGAGCCAGG TGAAGCGGCA GGAGGTCATC 
AGCGAGCTCC TGGTGACGGA GGCTGCCCAT GTGCGCATGC TCCGCGTGCT CCACGACGTC 
TTCTACCAGC CCATAGCGGA CGGGGGCTTC TACTCCCAGG AGGAGCTCCA GAACATCTTC 
CCCAGCCTGG ACGAGCTCAT CGAGGTGCAT TCCCTATTCC TTGATTCCCT GATGAAGCGG 
AGGCAGGACA GTGGCTACCT CATCAAGGAG ATCGGAGATG TGCTGCTGGC CCGGTTCGAT 
GGTGCCGAGG GCTCGTGGTT CCAGAAAATC TCCTCCCGCT TCTGTAGCCG CCAGTCGTTC 
GCCTTAGAGC AGCTCAAAGC CAAACAGCGC AAGGAGCCCC GGTTCTGTAC CTTCGTGCAG 
GAGGCCGAGA GCCGGCCCCG GTGCCGCCGG CTGCAGCTGA AGGACATGAT CCCCACCGAG 
ATGCAGCGTC TGACCAAGTA CCCACTGCTT CTGCAGAGCA TCGAGCAGAA CACAGAAGAG 
CCGGTGGAAC GGGAGAAAGT GGAGCGGGCA GCCGAGTGCT GCCGGGAAAT TCTGCAACAC 
GTCAACCTTG CCGTGCGGGA CATGGAGGAC CTGCTGCGGC TCAAAGATTA TCAGAGGCGT 
CTGGATTTGA CCCACCTGCG GCAGAGCAAC GACCCCATGC TGAGCGAGTT CAAGAACCTA 
GACATCACCA AGAAGAGGTT GATCTACGAG GGCCCACTGA CGTGGCGGGT GACGAAGGAC 
AAGGCTGTGG AGGTCCACGT GCTGCTGCTG GATGACCTGC TGCTGCTGCT TCAGCGCCAG 
GACGAGCGGT TCTTGCTTAA GTCGCACAGC CGGACGCTGA CGCCCACGCC CGATGGCAAG 
ACCATGCTGC GGCCAGTGCT GCGGCTCACC TCCGCCATGA CCCGCGAGGT GGCCACCGAT 
CACAAAGCCT TCTATGTCAT TTTTACCTGG GACCAGGAGG CCCAGATATA CGAGCTGGTG 
GCGCAGACGG TGTCTGAGCG GAGGATCTGG TGTACCCTGA TCACCGAGAC CGCTGGATGC 
CTGAAGGTCC CCTCCCGCAC CTCCCGACCC AAACACCGGC CCGGCCCCAG CAGCACTCGT 
GAACCCCTGC TTAGCAGCTC CGAAAACGGC AATGGCAGCC GAGAGATGCC CCCGGCAGAT 
GGCCGGATGG AGAGACTCTT CAACTCCCTC CTGCCCTTCT GCAGACAAGG CCTCGAGGGC 
CAGCTCGCCG CCAAGGCCCT TCAGAAAGTG CTGACCCTGA AACAGCTCCT GTTGCCTTCG 
GAGGAAGACA GCGGGGCAGG GCCTCCCCTC GAAGGGGATG GGGTCCCGGG GGGCAGCCCC 
TTGAGCCCAA CGCAGCCCCA GGAAATTCGG GAGGAGCTGC TCAGCCTGGA GGAGACCATT 
AAACAGCTAG AGGAGGTGGA GGAGGAATTT TGCCGCCTGA GACTCCTCCT GTCTCAGCTT 
GGGGAGAACA CCGTCCCCCA GTCTGGCTGT ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF1 ( XM_005219395.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005219452.1 Bos taurus Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OBa09522
Clone ID Related Accession (Same CDS sequence)	XM_010823706.2 , XM_005195333.3 , XM_005219394.3 , XM_010815120.2 , XM_005219395.3 , XM_005219394.4 , XM_005219395.4 , XM_005195332.3
Accession Version	XM_005219395.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2916bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rho guanine nucleotide exchange factor 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005219395.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCAG AAGACGTCGC CCGAGGGGCG GCCCCGGGGC CCCCCCGACC CGGCCTGGCG 
CCCGTCAGCA TCATTGGGGC AGAGGACGAG GATTTTGAGA TTGAGTTGAA GACGAACCCT 
GAGGAACAAA ACAACCAGTT CCAGAACCTG GATCAGGTGA AGCGGCGCCC GGCCCACCTC 
ATGGCCCTCC TGCAGCACGT GGCCATGCAG TTTGAGCCGG GACCCCTGCT CTGCTGCCTG 
CATGCGGACA TGCTGGGCTC CCTGGGCCCC AAGGAGTTCA AGAAAGCCTT CATCGACTTC 
TACCACAGCT TCCTGGATAA GCACTCGATT CTACGAGTGA TGGTCCCTCC TGCCGTGGCC 
TTTGAACTTG ACCGCACGCG GCCTGACCTC TTTCCTGAGG ATCTCCAGCG GCAGTTCGTG 
CAGGAGGTGG TGCAGAGCCA GCAGGTAGCC GTGGTCCGGC AGCTGGAGGA CTTCCGCTCC 
AAGAAGCTCA TGGGCATGAC ACCCTGGGAG CAGGAGCTGG TCCAGCTGGG GGCCTGGGTG 
GCGCGGGACC GTGCCAGCTA CGAGGCCCGG GAGCGGCACC TGGCTGAGCG GCAACTGACC 
CACCTGGAGG AGATGCAGCA CACCATCTCT AACGATGAGG AGAAGAGCGC CGCCGTGGTC 
AACGCCATCA GCATGTACAT GCGGCACCTT GGGGTGCGGA CCAAGAGTGG GGACAAGAAG 
TCCGGGAGGA ACTTCTTTCG GAAAAAGATG ATAGGGAACC GGCGGTCAGA TGAACCGACC 
AAGACCAAGA AAGGCCTGAG CAGCTTCCTG GATGCCGCCC GCTGGAACCG GGGAGAGCCC 
CAGGATTTTC GACACCCGAA GGTCGAAGTT GATGCTGAGA AGCCAGGCCT TACAGAACGT 
AAGGGAGGTC AGGGGGCGCC TTCCCGGGAC CGGAATCTCG GAGGCCCTGG GCAGGACACC 
CCCGGAGTTT CTCCGCACCT TCTGTCTGGG GACAGCCCTG ACCGGGAGCC AGGTGTTGAC 
GCCCTCCTGG AGGTGGGGGA CCCGCCCCCA CAGGGCCCAG CCAGCCTGGA CGCCCCAGCG 
CCGCTGGAGA GCACGGAGGA GGCTGCCGAC ACAGAAAGAA AGTGGAAGAG GCTGTCAGGG 
CGTCTGGGGC GCTCAGAGAG CCTGCGGGTG AGTGACCGCC GCCGGCCTTC CCGGGGTAGC 
CTCGGGGCTA AGGGCCGGGG TGGGGGCCGC TCCCGGAGCG ACGTGGACAT GGACCCCAGC 
TCCGCCACGG CCGTGCTTGG CCCTACCCGA CGAGCCACCC CGGAGCCTGG AGATGAGGGA 
GAGCCGGGCC GCTCAGGACT GGAGCTGGAA CCAGAGGAGC CGCCTGGCTG GCGAGAGCTT 
GTCCCCCAGG ACATTCTGCA CAGCCTGCCC AAGAGCCAGG TGAAGCGGCA GGAGGTCATC 
AGCGAGCTCC TGGTGACGGA GGCTGCCCAT GTGCGCATGC TCCGCGTGCT CCACGACGTC 
TTCTACCAGC CCATAGCGGA CGGGGGCTTC TACTCCCAGG AGGAGCTCCA GAACATCTTC 
CCCAGCCTGG ACGAGCTCAT CGAGGTGCAT TCCCTATTCC TTGATTCCCT GATGAAGCGG 
AGGCAGGACA GTGGCTACCT CATCAAGGAG ATCGGAGATG TGCTGCTGGC CCGGTTCGAT 
GGTGCCGAGG GCTCGTGGTT CCAGAAAATC TCCTCCCGCT TCTGTAGCCG CCAGTCGTTC 
GCCTTAGAGC AGCTCAAAGC CAAACAGCGC AAGGAGCCCC GGTTCTGTAC CTTCGTGCAG 
GAGGCCGAGA GCCGGCCCCG GTGCCGCCGG CTGCAGCTGA AGGACATGAT CCCCACCGAG 
ATGCAGCGTC TGACCAAGTA CCCACTGCTT CTGCAGAGCA TCGAGCAGAA CACAGAAGAG 
CCGGTGGAAC GGGAGAAAGT GGAGCGGGCA GCCGAGTGCT GCCGGGAAAT TCTGCAACAC 
GTCAACCTTG CCGTGCGGGA CATGGAGGAC CTGCTGCGGC TCAAAGATTA TCAGAGGCGT 
CTGGATTTGA CCCACCTGCG GCAGAGCAAC GACCCCATGC TGAGCGAGTT CAAGAACCTA 
GACATCACCA AGAAGAGGTT GATCTACGAG GGCCCACTGA CGTGGCGGGT GACGAAGGAC 
AAGGCTGTGG AGGTCCACGT GCTGCTGCTG GATGACCTGC TGCTGCTGCT TCAGCGCCAG 
GACGAGCGGT TCTTGCTTAA GTCGCACAGC CGGACGCTGA CGCCCACGCC CGATGGCAAG 
ACCATGCTGC GGCCAGTGCT GCGGCTCACC TCCGCCATGA CCCGCGAGGT GGCCACCGAT 
CACAAAGCCT TCTATGTCAT TTTTACCTGG GACCAGGAGG CCCAGATATA CGAGCTGGTG 
GCGCAGACGG TGTCTGAGCG GAGGATCTGG TGTACCCTGA TCACCGAGAC CGCTGGATGC 
CTGAAGGTCC CCTCCCGCAC CTCCCGACCC AAACACCGGC CCGGCCCCAG CAGCACTCGT 
GAACCCCTGC TTAGCAGCTC CGAAAACGGC AATGGCAGCC GAGAGATGCC CCCGGCAGAT 
GGCCGGATGG AGAGACTCTT CAACTCCCTC CTGCCCTTCT GCAGACAAGG CCTCGAGGGC 
CAGCTCGCCG CCAAGGCCCT TCAGAAAGTG CTGACCCTGA AACAGCTCCT GTTGCCTTCG 
GAGGAAGACA GCGGGGCAGG GCCTCCCCTC GAAGGGGATG GGGTCCCGGG GGGCAGCCCC 
TTGAGCCCAA CGCAGCCCCA GGAAATTCGG GAGGAGCTGC TCAGCCTGGA GGAGACCATT 
AAACAGCTAG AGGAGGTGGA GGAGGAATTT TGCCGCCTGA GACTCCTCCT GTCTCAGCTT 
GGGGAGAACA CCGTCCCCCA GTCTGGCTGT ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005219452.1
CDS	277..3192
Translation	MEAEDVARGAAPGPPRPGLAPVSIIGAEDEDFEIELKTNPEEQNNQFQNLDQVKRRPAHLMALLQHVAMQFEPGPLLCCLHADMLGSLGPKEFKKAFIDFYHSFLDKHSILRVMVPPAVAFELDRTRPDLFPEDLQRQFVQEVVQSQQVAVVRQLEDFRSKKLMGMTPWEQELVQLGAWVARDRASYEARERHLAERQLTHLEEMQHTISNDEEKSAAVVNAISMYMRHLGVRTKSGDKKSGRNFFRKKMIGNRRSDEPTKTKKGLSSFLDAARWNRGEPQDFRHPKVEVDAEKPGLTERKGGQGAPSRDRNLGGPGQDTPGVSPHLLSGDSPDREPGVDALLEVGDPPPQGPASLDAPAPLESTEEAADTERKWKRLSGRLGRSESLRVSDRRRPSRGSLGAKGRGGGRSRSDVDMDPSSATAVLGPTRRATPEPGDEGEPGRSGLELEPEEPPGWRELVPQDILHSLPKSQVKRQEVISELLVTEAAHVRMLRVLHDVFYQPIADGGFYSQEELQNIFPSLDELIEVHSLFLDSLMKRRQDSGYLIKEIGDVLLARFDGAEGSWFQKISSRFCSRQSFALEQLKAKQRKEPRFCTFVQEAESRPRCRRLQLKDMIPTEMQRLTKYPLLLQSIEQNTEEPVEREKVERAAECCREILQHVNLAVRDMEDLLRLKDYQRRLDLTHLRQSNDPMLSEFKNLDITKKRLIYEGPLTWRVTKDKAVEVHVLLLDDLLLLLQRQDERFLLKSHSRTLTPTPDGKTMLRPVLRLTSAMTREVATDHKAFYVIFTWDQEAQIYELVAQTVSERRIWCTLITETAGCLKVPSRTSRPKHRPGPSSTREPLLSSSENGNGSREMPPADGRMERLFNSLLPFCRQGLEGQLAAKALQKVLTLKQLLLPSEEDSGAGPPLEGDGVPGGSPLSPTQPQEIREELLSLEETIKQLEEVEEEFCRLRLLLSQLGENTVPQSGCT

Target ORF information:

RefSeq Version	XM_005219395.4
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005219395.4

ORF Insert Sequence:

ATGGAGGCAG AAGACGTCGC CCGAGGGGCG GCCCCGGGGC CCCCCCGACC CGGCCTGGCG 
CCCGTCAGCA TCATTGGGGC AGAGGACGAG GATTTTGAGA TTGAGTTGAA GACGAACCCT 
GAGGAACAAA ACAACCAGTT CCAGAACCTG GATCAGGTGA AGCGGCGCCC GGCCCACCTC 
ATGGCCCTCC TGCAGCACGT GGCCATGCAG TTTGAGCCGG GACCCCTGCT CTGCTGCCTG 
CATGCGGACA TGCTGGGCTC CCTGGGCCCC AAGGAGTTCA AGAAAGCCTT CATCGACTTC 
TACCACAGCT TCCTGGATAA GCACTCGATT CTACGAGTGA TGGTCCCTCC TGCCGTGGCC 
TTTGAACTTG ACCGCACGCG GCCTGACCTC TTTCCTGAGG ATCTCCAGCG GCAGTTCGTG 
CAGGAGGTGG TGCAGAGCCA GCAGGTAGCC GTGGTCCGGC AGCTGGAGGA CTTCCGCTCC 
AAGAAGCTCA TGGGCATGAC ACCCTGGGAG CAGGAGCTGG TCCAGCTGGG GGCCTGGGTG 
GCGCGGGACC GTGCCAGCTA CGAGGCCCGG GAGCGGCACC TGGCTGAGCG GCAACTGACC 
CACCTGGAGG AGATGCAGCA CACCATCTCT AACGATGAGG AGAAGAGCGC CGCCGTGGTC 
AACGCCATCA GCATGTACAT GCGGCACCTT GGGGTGCGGA CCAAGAGTGG GGACAAGAAG 
TCCGGGAGGA ACTTCTTTCG GAAAAAGATG ATAGGGAACC GGCGGTCAGA TGAACCGACC 
AAGACCAAGA AAGGCCTGAG CAGCTTCCTG GATGCCGCCC GCTGGAACCG GGGAGAGCCC 
CAGGATTTTC GACACCCGAA GGTCGAAGTT GATGCTGAGA AGCCAGGCCT TACAGAACGT 
AAGGGAGGTC AGGGGGCGCC TTCCCGGGAC CGGAATCTCG GAGGCCCTGG GCAGGACACC 
CCCGGAGTTT CTCCGCACCT TCTGTCTGGG GACAGCCCTG ACCGGGAGCC AGGTGTTGAC 
GCCCTCCTGG AGGTGGGGGA CCCGCCCCCA CAGGGCCCAG CCAGCCTGGA CGCCCCAGCG 
CCGCTGGAGA GCACGGAGGA GGCTGCCGAC ACAGAAAGAA AGTGGAAGAG GCTGTCAGGG 
CGTCTGGGGC GCTCAGAGAG CCTGCGGGTG AGTGACCGCC GCCGGCCTTC CCGGGGTAGC 
CTCGGGGCTA AGGGCCGGGG TGGGGGCCGC TCCCGGAGCG ACGTGGACAT GGACCCCAGC 
TCCGCCACGG CCGTGCTTGG CCCTACCCGA CGAGCCACCC CGGAGCCTGG AGATGAGGGA 
GAGCCGGGCC GCTCAGGACT GGAGCTGGAA CCAGAGGAGC CGCCTGGCTG GCGAGAGCTT 
GTCCCCCAGG ACATTCTGCA CAGCCTGCCC AAGAGCCAGG TGAAGCGGCA GGAGGTCATC 
AGCGAGCTCC TGGTGACGGA GGCTGCCCAT GTGCGCATGC TCCGCGTGCT CCACGACGTC 
TTCTACCAGC CCATAGCGGA CGGGGGCTTC TACTCCCAGG AGGAGCTCCA GAACATCTTC 
CCCAGCCTGG ACGAGCTCAT CGAGGTGCAT TCCCTATTCC TTGATTCCCT GATGAAGCGG 
AGGCAGGACA GTGGCTACCT CATCAAGGAG ATCGGAGATG TGCTGCTGGC CCGGTTCGAT 
GGTGCCGAGG GCTCGTGGTT CCAGAAAATC TCCTCCCGCT TCTGTAGCCG CCAGTCGTTC 
GCCTTAGAGC AGCTCAAAGC CAAACAGCGC AAGGAGCCCC GGTTCTGTAC CTTCGTGCAG 
GAGGCCGAGA GCCGGCCCCG GTGCCGCCGG CTGCAGCTGA AGGACATGAT CCCCACCGAG 
ATGCAGCGTC TGACCAAGTA CCCACTGCTT CTGCAGAGCA TCGAGCAGAA CACAGAAGAG 
CCGGTGGAAC GGGAGAAAGT GGAGCGGGCA GCCGAGTGCT GCCGGGAAAT TCTGCAACAC 
GTCAACCTTG CCGTGCGGGA CATGGAGGAC CTGCTGCGGC TCAAAGATTA TCAGAGGCGT 
CTGGATTTGA CCCACCTGCG GCAGAGCAAC GACCCCATGC TGAGCGAGTT CAAGAACCTA 
GACATCACCA AGAAGAGGTT GATCTACGAG GGCCCACTGA CGTGGCGGGT GACGAAGGAC 
AAGGCTGTGG AGGTCCACGT GCTGCTGCTG GATGACCTGC TGCTGCTGCT TCAGCGCCAG 
GACGAGCGGT TCTTGCTTAA GTCGCACAGC CGGACGCTGA CGCCCACGCC CGATGGCAAG 
ACCATGCTGC GGCCAGTGCT GCGGCTCACC TCCGCCATGA CCCGCGAGGT GGCCACCGAT 
CACAAAGCCT TCTATGTCAT TTTTACCTGG GACCAGGAGG CCCAGATATA CGAGCTGGTG 
GCGCAGACGG TGTCTGAGCG GAGGATCTGG TGTACCCTGA TCACCGAGAC CGCTGGATGC 
CTGAAGGTCC CCTCCCGCAC CTCCCGACCC AAACACCGGC CCGGCCCCAG CAGCACTCGT 
GAACCCCTGC TTAGCAGCTC CGAAAACGGC AATGGCAGCC GAGAGATGCC CCCGGCAGAT 
GGCCGGATGG AGAGACTCTT CAACTCCCTC CTGCCCTTCT GCAGACAAGG CCTCGAGGGC 
CAGCTCGCCG CCAAGGCCCT TCAGAAAGTG CTGACCCTGA AACAGCTCCT GTTGCCTTCG 
GAGGAAGACA GCGGGGCAGG GCCTCCCCTC GAAGGGGATG GGGTCCCGGG GGGCAGCCCC 
TTGAGCCCAA CGCAGCCCCA GGAAATTCGG GAGGAGCTGC TCAGCCTGGA GGAGACCATT 
AAACAGCTAG AGGAGGTGGA GGAGGAATTT TGCCGCCTGA GACTCCTCCT GTCTCAGCTT 
GGGGAGAACA CCGTCCCCCA GTCTGGCTGT ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF1 ( XM_005195332.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005195389.1 Bos taurus Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa09522
Clone ID Related Accession (Same CDS sequence)	XM_010823706.2 , XM_005195333.3 , XM_005219394.3 , XM_010815120.2 , XM_005219395.3 , XM_005219394.4 , XM_005219395.4 , XM_005195332.3
Accession Version	XM_005195332.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2916bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	rho guanine nucleotide exchange factor 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646390.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005195332.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCAG AAGACGTCGC CCGAGGGGCG GCCCCGGGGC CCCCCCGACC CGGCCTGGCG 
CCCGTCAGCA TCATTGGGGC AGAGGACGAG GATTTTGAGA TTGAGTTGAA GACGAACCCT 
GAGGAACAAA ACAACCAGTT CCAGAACCTG GATCAGGTGA AGCGGCGCCC GGCCCACCTC 
ATGGCCCTCC TGCAGCACGT GGCCATGCAG TTTGAGCCGG GACCCCTGCT CTGCTGCCTG 
CATGCGGACA TGCTGGGCTC CCTGGGCCCC AAGGAGTTCA AGAAAGCCTT CATCGACTTC 
TACCACAGCT TCCTGGATAA GCACTCGATT CTACGAGTGA TGGTCCCTCC TGCCGTGGCC 
TTTGAACTTG ACCGCACGCG GCCTGACCTC TTTCCTGAGG ATCTCCAGCG GCAGTTCGTG 
CAGGAGGTGG TGCAGAGCCA GCAGGTAGCC GTGGTCCGGC AGCTGGAGGA CTTCCGCTCC 
AAGAAGCTCA TGGGCATGAC ACCCTGGGAG CAGGAGCTGG TCCAGCTGGG GGCCTGGGTG 
GCGCGGGACC GTGCCAGCTA CGAGGCCCGG GAGCGGCACC TGGCTGAGCG GCAACTGACC 
CACCTGGAGG AGATGCAGCA CACCATCTCT AACGATGAGG AGAAGAGCGC CGCCGTGGTC 
AACGCCATCA GCATGTACAT GCGGCACCTT GGGGTGCGGA CCAAGAGTGG GGACAAGAAG 
TCCGGGAGGA ACTTCTTTCG GAAAAAGATG ATAGGGAACC GGCGGTCAGA TGAACCGACC 
AAGACCAAGA AAGGCCTGAG CAGCTTCCTG GATGCCGCCC GCTGGAACCG GGGAGAGCCC 
CAGGATTTTC GACACCCGAA GGTCGAAGTT GATGCTGAGA AGCCAGGCCT TACAGAACGT 
AAGGGAGGTC AGGGGGCGCC TTCCCGGGAC CGGAATCTCG GAGGCCCTGG GCAGGACACC 
CCCGGAGTTT CTCCGCACCT TCTGTCTGGG GACAGCCCTG ACCGGGAGCC AGGTGTTGAC 
GCCCTCCTGG AGGTGGGGGA CCCGCCCCCA CAGGGCCCAG CCAGCCTGGA CGCCCCAGCG 
CCGCTGGAGA GCACGGAGGA GGCTGCCGAC ACAGAAAGAA AGTGGAAGAG GCTGTCAGGG 
CGTCTGGGGC GCTCAGAGAG CCTGCGGGTG AGTGACCGCC GCCGGCCTTC CCGGGGTAGC 
CTCGGGGCTA AGGGCCGGGG TGGGGGCCGC TCCCGGAGCG ACGTGGACAT GGACCCCAGC 
TCCGCCACGG CCGTGCTTGG CCCTACCCGA CGAGCCACCC CGGAGCCTGG AGATGAGGGA 
GAGCCGGGCC GCTCAGGACT GGAGCTGGAA CCAGAGGAGC CGCCTGGCTG GCGAGAGCTT 
GTCCCCCAGG ACATTCTGCA CAGCCTGCCC AAGAGCCAGG TGAAGCGGCA GGAGGTCATC 
AGCGAGCTCC TGGTGACGGA GGCTGCCCAT GTGCGCATGC TCCGCGTGCT CCACGACGTC 
TTCTACCAGC CCATAGCGGA CGGGGGCTTC TACTCCCAGG AGGAGCTCCA GAACATCTTC 
CCCAGCCTGG ACGAGCTCAT CGAGGTGCAT TCCCTATTCC TTGATTCCCT GATGAAGCGG 
AGGCAGGACA GTGGCTACCT CATCAAGGAG ATCGGAGATG TGCTGCTGGC CCGGTTCGAT 
GGTGCCGAGG GCTCGTGGTT CCAGAAAATC TCCTCCCGCT TCTGTAGCCG CCAGTCGTTC 
GCCTTAGAGC AGCTCAAAGC CAAACAGCGC AAGGAGCCCC GGTTCTGTAC CTTCGTGCAG 
GAGGCCGAGA GCCGGCCCCG GTGCCGCCGG CTGCAGCTGA AGGACATGAT CCCCACCGAG 
ATGCAGCGTC TGACCAAGTA CCCACTGCTT CTGCAGAGCA TCGAGCAGAA CACAGAAGAG 
CCGGTGGAAC GGGAGAAAGT GGAGCGGGCA GCCGAGTGCT GCCGGGAAAT TCTGCAACAC 
GTCAACCTTG CCGTGCGGGA CATGGAGGAC CTGCTGCGGC TCAAAGATTA TCAGAGGCGT 
CTGGATTTGA CCCACCTGCG GCAGAGCAAC GACCCCATGC TGAGCGAGTT CAAGAACCTA 
GACATCACCA AGAAGAGGTT GATCTACGAG GGCCCACTGA CGTGGCGGGT GACGAAGGAC 
AAGGCTGTGG AGGTCCACGT GCTGCTGCTG GATGACCTGC TGCTGCTGCT TCAGCGCCAG 
GACGAGCGGT TCTTGCTTAA GTCGCACAGC CGGACGCTGA CGCCCACGCC CGATGGCAAG 
ACCATGCTGC GGCCAGTGCT GCGGCTCACC TCCGCCATGA CCCGCGAGGT GGCCACCGAT 
CACAAAGCCT TCTATGTCAT TTTTACCTGG GACCAGGAGG CCCAGATATA CGAGCTGGTG 
GCGCAGACGG TGTCTGAGCG GAGGATCTGG TGTACCCTGA TCACCGAGAC CGCTGGATGC 
CTGAAGGTCC CCTCCCGCAC CTCCCGACCC AAACACCGGC CCGGCCCCAG CAGCACTCGT 
GAACCCCTGC TTAGCAGCTC CGAAAACGGC AATGGCAGCC GAGAGATGCC CCCGGCAGAT 
GGCCGGATGG AGAGACTCTT CAACTCCCTC CTGCCCTTCT GCAGACAAGG CCTCGAGGGC 
CAGCTCGCCG CCAAGGCCCT TCAGAAAGTG CTGACCCTGA AACAGCTCCT GTTGCCTTCG 
GAGGAAGACA GCGGGGCAGG GCCTCCCCTC GAAGGGGATG GGGTCCCGGG GGGCAGCCCC 
TTGAGCCCAA CGCAGCCCCA GGAAATTCGG GAGGAGCTGC TCAGCCTGGA GGAGACCATT 
AAACAGCTAG AGGAGGTGGA GGAGGAATTT TGCCGCCTGA GACTCCTCCT GTCTCAGCTT 
GGGGAGAACA CCGTCCCCCA GTCTGGCTGT ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005195389.1
CDS	470..3385
Translation	MEAEDVARGAAPGPPRPGLAPVSIIGAEDEDFEIELKTNPEEQNNQFQNLDQVKRRPAHLMALLQHVAMQFEPGPLLCCLHADMLGSLGPKEFKKAFIDFYHSFLDKHSILRVMVPPAVAFELDRTRPDLFPEDLQRQFVQEVVQSQQVAVVRQLEDFRSKKLMGMTPWEQELVQLGAWVARDRASYEARERHLAERQLTHLEEMQHTISNDEEKSAAVVNAISMYMRHLGVRTKSGDKKSGRNFFRKKMIGNRRSDEPTKTKKGLSSFLDAARWNRGEPQDFRHPKVEVDAEKPGLTERKGGQGAPSRDRNLGGPGQDTPGVSPHLLSGDSPDREPGVDALLEVGDPPPQGPASLDAPAPLESTEEAADTERKWKRLSGRLGRSESLRVSDRRRPSRGSLGAKGRGGGRSRSDVDMDPSSATAVLGPTRRATPEPGDEGEPGRSGLELEPEEPPGWRELVPQDILHSLPKSQVKRQEVISELLVTEAAHVRMLRVLHDVFYQPIADGGFYSQEELQNIFPSLDELIEVHSLFLDSLMKRRQDSGYLIKEIGDVLLARFDGAEGSWFQKISSRFCSRQSFALEQLKAKQRKEPRFCTFVQEAESRPRCRRLQLKDMIPTEMQRLTKYPLLLQSIEQNTEEPVEREKVERAAECCREILQHVNLAVRDMEDLLRLKDYQRRLDLTHLRQSNDPMLSEFKNLDITKKRLIYEGPLTWRVTKDKAVEVHVLLLDDLLLLLQRQDERFLLKSHSRTLTPTPDGKTMLRPVLRLTSAMTREVATDHKAFYVIFTWDQEAQIYELVAQTVSERRIWCTLITETAGCLKVPSRTSRPKHRPGPSSTREPLLSSSENGNGSREMPPADGRMERLFNSLLPFCRQGLEGQLAAKALQKVLTLKQLLLPSEEDSGAGPPLEGDGVPGGSPLSPTQPQEIREELLSLEETIKQLEEVEEEFCRLRLLLSQLGENTVPQSGCT

Target ORF information:

RefSeq Version	XM_005195332.3
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005195332.3

ORF Insert Sequence:

ATGGAGGCAG AAGACGTCGC CCGAGGGGCG GCCCCGGGGC CCCCCCGACC CGGCCTGGCG 
CCCGTCAGCA TCATTGGGGC AGAGGACGAG GATTTTGAGA TTGAGTTGAA GACGAACCCT 
GAGGAACAAA ACAACCAGTT CCAGAACCTG GATCAGGTGA AGCGGCGCCC GGCCCACCTC 
ATGGCCCTCC TGCAGCACGT GGCCATGCAG TTTGAGCCGG GACCCCTGCT CTGCTGCCTG 
CATGCGGACA TGCTGGGCTC CCTGGGCCCC AAGGAGTTCA AGAAAGCCTT CATCGACTTC 
TACCACAGCT TCCTGGATAA GCACTCGATT CTACGAGTGA TGGTCCCTCC TGCCGTGGCC 
TTTGAACTTG ACCGCACGCG GCCTGACCTC TTTCCTGAGG ATCTCCAGCG GCAGTTCGTG 
CAGGAGGTGG TGCAGAGCCA GCAGGTAGCC GTGGTCCGGC AGCTGGAGGA CTTCCGCTCC 
AAGAAGCTCA TGGGCATGAC ACCCTGGGAG CAGGAGCTGG TCCAGCTGGG GGCCTGGGTG 
GCGCGGGACC GTGCCAGCTA CGAGGCCCGG GAGCGGCACC TGGCTGAGCG GCAACTGACC 
CACCTGGAGG AGATGCAGCA CACCATCTCT AACGATGAGG AGAAGAGCGC CGCCGTGGTC 
AACGCCATCA GCATGTACAT GCGGCACCTT GGGGTGCGGA CCAAGAGTGG GGACAAGAAG 
TCCGGGAGGA ACTTCTTTCG GAAAAAGATG ATAGGGAACC GGCGGTCAGA TGAACCGACC 
AAGACCAAGA AAGGCCTGAG CAGCTTCCTG GATGCCGCCC GCTGGAACCG GGGAGAGCCC 
CAGGATTTTC GACACCCGAA GGTCGAAGTT GATGCTGAGA AGCCAGGCCT TACAGAACGT 
AAGGGAGGTC AGGGGGCGCC TTCCCGGGAC CGGAATCTCG GAGGCCCTGG GCAGGACACC 
CCCGGAGTTT CTCCGCACCT TCTGTCTGGG GACAGCCCTG ACCGGGAGCC AGGTGTTGAC 
GCCCTCCTGG AGGTGGGGGA CCCGCCCCCA CAGGGCCCAG CCAGCCTGGA CGCCCCAGCG 
CCGCTGGAGA GCACGGAGGA GGCTGCCGAC ACAGAAAGAA AGTGGAAGAG GCTGTCAGGG 
CGTCTGGGGC GCTCAGAGAG CCTGCGGGTG AGTGACCGCC GCCGGCCTTC CCGGGGTAGC 
CTCGGGGCTA AGGGCCGGGG TGGGGGCCGC TCCCGGAGCG ACGTGGACAT GGACCCCAGC 
TCCGCCACGG CCGTGCTTGG CCCTACCCGA CGAGCCACCC CGGAGCCTGG AGATGAGGGA 
GAGCCGGGCC GCTCAGGACT GGAGCTGGAA CCAGAGGAGC CGCCTGGCTG GCGAGAGCTT 
GTCCCCCAGG ACATTCTGCA CAGCCTGCCC AAGAGCCAGG TGAAGCGGCA GGAGGTCATC 
AGCGAGCTCC TGGTGACGGA GGCTGCCCAT GTGCGCATGC TCCGCGTGCT CCACGACGTC 
TTCTACCAGC CCATAGCGGA CGGGGGCTTC TACTCCCAGG AGGAGCTCCA GAACATCTTC 
CCCAGCCTGG ACGAGCTCAT CGAGGTGCAT TCCCTATTCC TTGATTCCCT GATGAAGCGG 
AGGCAGGACA GTGGCTACCT CATCAAGGAG ATCGGAGATG TGCTGCTGGC CCGGTTCGAT 
GGTGCCGAGG GCTCGTGGTT CCAGAAAATC TCCTCCCGCT TCTGTAGCCG CCAGTCGTTC 
GCCTTAGAGC AGCTCAAAGC CAAACAGCGC AAGGAGCCCC GGTTCTGTAC CTTCGTGCAG 
GAGGCCGAGA GCCGGCCCCG GTGCCGCCGG CTGCAGCTGA AGGACATGAT CCCCACCGAG 
ATGCAGCGTC TGACCAAGTA CCCACTGCTT CTGCAGAGCA TCGAGCAGAA CACAGAAGAG 
CCGGTGGAAC GGGAGAAAGT GGAGCGGGCA GCCGAGTGCT GCCGGGAAAT TCTGCAACAC 
GTCAACCTTG CCGTGCGGGA CATGGAGGAC CTGCTGCGGC TCAAAGATTA TCAGAGGCGT 
CTGGATTTGA CCCACCTGCG GCAGAGCAAC GACCCCATGC TGAGCGAGTT CAAGAACCTA 
GACATCACCA AGAAGAGGTT GATCTACGAG GGCCCACTGA CGTGGCGGGT GACGAAGGAC 
AAGGCTGTGG AGGTCCACGT GCTGCTGCTG GATGACCTGC TGCTGCTGCT TCAGCGCCAG 
GACGAGCGGT TCTTGCTTAA GTCGCACAGC CGGACGCTGA CGCCCACGCC CGATGGCAAG 
ACCATGCTGC GGCCAGTGCT GCGGCTCACC TCCGCCATGA CCCGCGAGGT GGCCACCGAT 
CACAAAGCCT TCTATGTCAT TTTTACCTGG GACCAGGAGG CCCAGATATA CGAGCTGGTG 
GCGCAGACGG TGTCTGAGCG GAGGATCTGG TGTACCCTGA TCACCGAGAC CGCTGGATGC 
CTGAAGGTCC CCTCCCGCAC CTCCCGACCC AAACACCGGC CCGGCCCCAG CAGCACTCGT 
GAACCCCTGC TTAGCAGCTC CGAAAACGGC AATGGCAGCC GAGAGATGCC CCCGGCAGAT 
GGCCGGATGG AGAGACTCTT CAACTCCCTC CTGCCCTTCT GCAGACAAGG CCTCGAGGGC 
CAGCTCGCCG CCAAGGCCCT TCAGAAAGTG CTGACCCTGA AACAGCTCCT GTTGCCTTCG 
GAGGAAGACA GCGGGGCAGG GCCTCCCCTC GAAGGGGATG GGGTCCCGGG GGGCAGCCCC 
TTGAGCCCAA CGCAGCCCCA GGAAATTCGG GAGGAGCTGC TCAGCCTGGA GGAGACCATT 
AAACAGCTAG AGGAGGTGGA GGAGGAATTT TGCCGCCTGA GACTCCTCCT GTCTCAGCTT 
GGGGAGAACA CCGTCCCCCA GTCTGGCTGT ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF1 ( XM_010815115.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010813417.1 Bos taurus Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa48599
Clone ID Related Accession (Same CDS sequence)	XM_010815115.2 , XM_010815115.3 , XM_010823701.2
Accession Version	XM_010815115.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2967bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	rho guanine nucleotide exchange factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104466.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010815115.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGAGA GAGGCGAGAC AGCGAGCGAC ACGGACAGCC CGGCAGTGCC CATGGAGGCA 
GAAGACGTCG CCCGAGGGGC GGCCCCGGGG CCCCCCCGAC CCGGCCTGGC GCCCGTCAGC 
ATCATTGGGG CAGAGGACGA GGATTTTGAG ATTGAGTTGA AGACGAACCC TGAGGAACAA 
AACAACCAGT TCCAGAACCT GGATCAGGTG AAGCGGCGCC CGGCCCACCT CATGGCCCTC 
CTGCAGCACG TGGCCATGCA GTTTGAGCCG GGACCCCTGC TCTGCTGCCT GCATGCGGAC 
ATGCTGGGCT CCCTGGGCCC CAAGGAGTTC AAGAAAGCCT TCATCGACTT CTACCACAGC 
TTCCTGGATA AGCACTCGAT TCTACGAGTG ATGGTCCCTC CTGCCGTGGC CTTTGAACTT 
GACCGCACGC GGCCTGACCT CTTTCCTGAG GATCTCCAGC GGCAGTTCGT GCAGGAGGTG 
GTGCAGAGCC AGCAGGTAGC CGTGGTCCGG CAGCTGGAGG ACTTCCGCTC CAAGAAGCTC 
ATGGGCATGA CACCCTGGGA GCAGGAGCTG GTCCAGCTGG GGGCCTGGGT GGCGCGGGAC 
CGTGCCAGCT ACGAGGCCCG GGAGCGGCAC CTGGCTGAGC GGCAACTGAC CCACCTGGAG 
GAGATGCAGC ACACCATCTC TAACGATGAG GAGAAGAGCG CCGCCGTGGT CAACGCCATC 
AGCATGTACA TGCGGCACCT TGGGGTGCGG ACCAAGAGTG GGGACAAGAA GTCCGGGAGG 
AACTTCTTTC GGAAAAAGAT GATAGGGAAC CGGCGGTCAG ATGAACCGAC CAAGACCAAG 
AAAGGCCTGA GCAGCTTCCT GGATGCCGCC CGCTGGAACC GGGGAGAGCC CCAGGATTTT 
CGACACCCGA AGGTCGAAGT TGATGCTGAG AAGCCAGGCC TTACAGAACG TAAGGGAGGT 
CAGGGGGCGC CTTCCCGGGA CCGGAATCTC GGAGGCCCTG GGCAGGACAC CCCCGGAGTT 
TCTCCGCACC TTCTGTCTGG GGACAGCCCT GACCGGGAGC CAGGTGTTGA CGCCCTCCTG 
GAGGTGGGGG ACCCGCCCCC ACAGGGCCCA GCCAGCCTGG ACGCCCCAGC GCCGCTGGAG 
AGCACGGAGG AGGCTGCCGA CACAGAAAGA AAGTGGAAGA GGCTGTCAGG GCGTCTGGGG 
CGCTCAGAGA GCCTGCGGGT GAGTGACCGC CGCCGGCCTT CCCGGGGTAG CCTCGGGGCT 
AAGGGCCGGG GTGGGGGCCG CTCCCGGAGC GACGTGGACA TGGACCCCAG CTCCGCCACG 
GCCGTGCTTG GCCCTACCCG ACGAGCCACC CCGGAGCCTG GAGATGAGGG AGAGCCGGGC 
CGCTCAGGAC TGGAGCTGGA ACCAGAGGAG CCGCCTGGCT GGCGAGAGCT TGTCCCCCAG 
GACATTCTGC ACAGCCTGCC CAAGAGCCAG GTGAAGCGGC AGGAGGTCAT CAGCGAGCTC 
CTGGTGACGG AGGCTGCCCA TGTGCGCATG CTCCGCGTGC TCCACGACGT CTTCTACCAG 
CCCATAGCGG ACGGGGGCTT CTACTCCCAG GAGGAGCTCC AGAACATCTT CCCCAGCCTG 
GACGAGCTCA TCGAGGTGCA TTCCCTATTC CTTGATTCCC TGATGAAGCG GAGGCAGGAC 
AGTGGCTACC TCATCAAGGA GATCGGAGAT GTGCTGCTGG CCCGGTTCGA TGGTGCCGAG 
GGCTCGTGGT TCCAGAAAAT CTCCTCCCGC TTCTGTAGCC GCCAGTCGTT CGCCTTAGAG 
CAGCTCAAAG CCAAACAGCG CAAGGAGCCC CGGTTCTGTA CCTTCGTGCA GGAGGCCGAG 
AGCCGGCCCC GGTGCCGCCG GCTGCAGCTG AAGGACATGA TCCCCACCGA GATGCAGCGT 
CTGACCAAGT ACCCACTGCT TCTGCAGAGC ATCGAGCAGA ACACAGAAGA GCCGGTGGAA 
CGGGAGAAAG TGGAGCGGGC AGCCGAGTGC TGCCGGGAAA TTCTGCAACA CGTCAACCTT 
GCCGTGCGGG ACATGGAGGA CCTGCTGCGG CTCAAAGATT ATCAGAGGCG TCTGGATTTG 
ACCCACCTGC GGCAGAGCAA CGACCCCATG CTGAGCGAGT TCAAGAACCT AGACATCACC 
AAGAAGAGGT TGATCTACGA GGGCCCACTG ACGTGGCGGG TGACGAAGGA CAAGGCTGTG 
GAGGTCCACG TGCTGCTGCT GGATGACCTG CTGCTGCTGC TTCAGCGCCA GGACGAGCGG 
TTCTTGCTTA AGTCGCACAG CCGGACGCTG ACGCCCACGC CCGATGGCAA GACCATGCTG 
CGGCCAGTGC TGCGGCTCAC CTCCGCCATG ACCCGCGAGG TGGCCACCGA TCACAAAGCC 
TTCTATGTCA TTTTTACCTG GGACCAGGAG GCCCAGATAT ACGAGCTGGT GGCGCAGACG 
GTGTCTGAGC GGAGGATCTG GTGTACCCTG ATCACCGAGA CCGCTGGATG CCTGAAGGTC 
CCCTCCCGCA CCTCCCGACC CAAACACCGG CCCGGCCCCA GCAGCACTCG TGAACCCCTG 
CTTAGCAGCT CCGAAAACGG CAATGGCAGC CGAGAGATGC CCCCGGCAGA TGGCCGGATG 
GAGAGACTCT TCAACTCCCT CCTGCCCTTC TGCAGACAAG GCCTCGAGGG CCAGCTCGCC 
GCCAAGGCCC TTCAGAAAGT GCTGACCCTG AAACAGCTCC TGTTGCCTTC GGAGGAAGAC 
AGCGGGGCAG GGCCTCCCCT CGAAGGGGAT GGGGTCCCGG GGGGCAGCCC CTTGAGCCCA 
ACGCAGCCCC AGGAAATTCG GGAGGAGCTG CTCAGCCTGG AGGAGACCAT TAAACAGCTA 
GAGGAGGTGG AGGAGGAATT TTGCCGCCTG AGACTCCTCC TGTCTCAGCT TGGGGAGAAC 
ACCGTCCCCC AGTCTGGCTG TACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010813417.1
CDS	139..3105
Translation	MGERGETASDTDSPAVPMEAEDVARGAAPGPPRPGLAPVSIIGAEDEDFEIELKTNPEEQNNQFQNLDQVKRRPAHLMALLQHVAMQFEPGPLLCCLHADMLGSLGPKEFKKAFIDFYHSFLDKHSILRVMVPPAVAFELDRTRPDLFPEDLQRQFVQEVVQSQQVAVVRQLEDFRSKKLMGMTPWEQELVQLGAWVARDRASYEARERHLAERQLTHLEEMQHTISNDEEKSAAVVNAISMYMRHLGVRTKSGDKKSGRNFFRKKMIGNRRSDEPTKTKKGLSSFLDAARWNRGEPQDFRHPKVEVDAEKPGLTERKGGQGAPSRDRNLGGPGQDTPGVSPHLLSGDSPDREPGVDALLEVGDPPPQGPASLDAPAPLESTEEAADTERKWKRLSGRLGRSESLRVSDRRRPSRGSLGAKGRGGGRSRSDVDMDPSSATAVLGPTRRATPEPGDEGEPGRSGLELEPEEPPGWRELVPQDILHSLPKSQVKRQEVISELLVTEAAHVRMLRVLHDVFYQPIADGGFYSQEELQNIFPSLDELIEVHSLFLDSLMKRRQDSGYLIKEIGDVLLARFDGAEGSWFQKISSRFCSRQSFALEQLKAKQRKEPRFCTFVQEAESRPRCRRLQLKDMIPTEMQRLTKYPLLLQSIEQNTEEPVEREKVERAAECCREILQHVNLAVRDMEDLLRLKDYQRRLDLTHLRQSNDPMLSEFKNLDITKKRLIYEGPLTWRVTKDKAVEVHVLLLDDLLLLLQRQDERFLLKSHSRTLTPTPDGKTMLRPVLRLTSAMTREVATDHKAFYVIFTWDQEAQIYELVAQTVSERRIWCTLITETAGCLKVPSRTSRPKHRPGPSSTREPLLSSSENGNGSREMPPADGRMERLFNSLLPFCRQGLEGQLAAKALQKVLTLKQLLLPSEEDSGAGPPLEGDGVPGGSPLSPTQPQEIREELLSLEETIKQLEEVEEEFCRLRLLLSQLGENTVPQSGCT

Target ORF information:

RefSeq Version	XM_010815115.2
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010815115.2

ORF Insert Sequence:

ATGGGAGAGA GAGGCGAGAC AGCGAGCGAC ACGGACAGCC CGGCAGTGCC CATGGAGGCA 
GAAGACGTCG CCCGAGGGGC GGCCCCGGGG CCCCCCCGAC CCGGCCTGGC GCCCGTCAGC 
ATCATTGGGG CAGAGGACGA GGATTTTGAG ATTGAGTTGA AGACGAACCC TGAGGAACAA 
AACAACCAGT TCCAGAACCT GGATCAGGTG AAGCGGCGCC CGGCCCACCT CATGGCCCTC 
CTGCAGCACG TGGCCATGCA GTTTGAGCCG GGACCCCTGC TCTGCTGCCT GCATGCGGAC 
ATGCTGGGCT CCCTGGGCCC CAAGGAGTTC AAGAAAGCCT TCATCGACTT CTACCACAGC 
TTCCTGGATA AGCACTCGAT TCTACGAGTG ATGGTCCCTC CTGCCGTGGC CTTTGAACTT 
GACCGCACGC GGCCTGACCT CTTTCCTGAG GATCTCCAGC GGCAGTTCGT GCAGGAGGTG 
GTGCAGAGCC AGCAGGTAGC CGTGGTCCGG CAGCTGGAGG ACTTCCGCTC CAAGAAGCTC 
ATGGGCATGA CACCCTGGGA GCAGGAGCTG GTCCAGCTGG GGGCCTGGGT GGCGCGGGAC 
CGTGCCAGCT ACGAGGCCCG GGAGCGGCAC CTGGCTGAGC GGCAACTGAC CCACCTGGAG 
GAGATGCAGC ACACCATCTC TAACGATGAG GAGAAGAGCG CCGCCGTGGT CAACGCCATC 
AGCATGTACA TGCGGCACCT TGGGGTGCGG ACCAAGAGTG GGGACAAGAA GTCCGGGAGG 
AACTTCTTTC GGAAAAAGAT GATAGGGAAC CGGCGGTCAG ATGAACCGAC CAAGACCAAG 
AAAGGCCTGA GCAGCTTCCT GGATGCCGCC CGCTGGAACC GGGGAGAGCC CCAGGATTTT 
CGACACCCGA AGGTCGAAGT TGATGCTGAG AAGCCAGGCC TTACAGAACG TAAGGGAGGT 
CAGGGGGCGC CTTCCCGGGA CCGGAATCTC GGAGGCCCTG GGCAGGACAC CCCCGGAGTT 
TCTCCGCACC TTCTGTCTGG GGACAGCCCT GACCGGGAGC CAGGTGTTGA CGCCCTCCTG 
GAGGTGGGGG ACCCGCCCCC ACAGGGCCCA GCCAGCCTGG ACGCCCCAGC GCCGCTGGAG 
AGCACGGAGG AGGCTGCCGA CACAGAAAGA AAGTGGAAGA GGCTGTCAGG GCGTCTGGGG 
CGCTCAGAGA GCCTGCGGGT GAGTGACCGC CGCCGGCCTT CCCGGGGTAG CCTCGGGGCT 
AAGGGCCGGG GTGGGGGCCG CTCCCGGAGC GACGTGGACA TGGACCCCAG CTCCGCCACG 
GCCGTGCTTG GCCCTACCCG ACGAGCCACC CCGGAGCCTG GAGATGAGGG AGAGCCGGGC 
CGCTCAGGAC TGGAGCTGGA ACCAGAGGAG CCGCCTGGCT GGCGAGAGCT TGTCCCCCAG 
GACATTCTGC ACAGCCTGCC CAAGAGCCAG GTGAAGCGGC AGGAGGTCAT CAGCGAGCTC 
CTGGTGACGG AGGCTGCCCA TGTGCGCATG CTCCGCGTGC TCCACGACGT CTTCTACCAG 
CCCATAGCGG ACGGGGGCTT CTACTCCCAG GAGGAGCTCC AGAACATCTT CCCCAGCCTG 
GACGAGCTCA TCGAGGTGCA TTCCCTATTC CTTGATTCCC TGATGAAGCG GAGGCAGGAC 
AGTGGCTACC TCATCAAGGA GATCGGAGAT GTGCTGCTGG CCCGGTTCGA TGGTGCCGAG 
GGCTCGTGGT TCCAGAAAAT CTCCTCCCGC TTCTGTAGCC GCCAGTCGTT CGCCTTAGAG 
CAGCTCAAAG CCAAACAGCG CAAGGAGCCC CGGTTCTGTA CCTTCGTGCA GGAGGCCGAG 
AGCCGGCCCC GGTGCCGCCG GCTGCAGCTG AAGGACATGA TCCCCACCGA GATGCAGCGT 
CTGACCAAGT ACCCACTGCT TCTGCAGAGC ATCGAGCAGA ACACAGAAGA GCCGGTGGAA 
CGGGAGAAAG TGGAGCGGGC AGCCGAGTGC TGCCGGGAAA TTCTGCAACA CGTCAACCTT 
GCCGTGCGGG ACATGGAGGA CCTGCTGCGG CTCAAAGATT ATCAGAGGCG TCTGGATTTG 
ACCCACCTGC GGCAGAGCAA CGACCCCATG CTGAGCGAGT TCAAGAACCT AGACATCACC 
AAGAAGAGGT TGATCTACGA GGGCCCACTG ACGTGGCGGG TGACGAAGGA CAAGGCTGTG 
GAGGTCCACG TGCTGCTGCT GGATGACCTG CTGCTGCTGC TTCAGCGCCA GGACGAGCGG 
TTCTTGCTTA AGTCGCACAG CCGGACGCTG ACGCCCACGC CCGATGGCAA GACCATGCTG 
CGGCCAGTGC TGCGGCTCAC CTCCGCCATG ACCCGCGAGG TGGCCACCGA TCACAAAGCC 
TTCTATGTCA TTTTTACCTG GGACCAGGAG GCCCAGATAT ACGAGCTGGT GGCGCAGACG 
GTGTCTGAGC GGAGGATCTG GTGTACCCTG ATCACCGAGA CCGCTGGATG CCTGAAGGTC 
CCCTCCCGCA CCTCCCGACC CAAACACCGG CCCGGCCCCA GCAGCACTCG TGAACCCCTG 
CTTAGCAGCT CCGAAAACGG CAATGGCAGC CGAGAGATGC CCCCGGCAGA TGGCCGGATG 
GAGAGACTCT TCAACTCCCT CCTGCCCTTC TGCAGACAAG GCCTCGAGGG CCAGCTCGCC 
GCCAAGGCCC TTCAGAAAGT GCTGACCCTG AAACAGCTCC TGTTGCCTTC GGAGGAAGAC 
AGCGGGGCAG GGCCTCCCCT CGAAGGGGAT GGGGTCCCGG GGGGCAGCCC CTTGAGCCCA 
ACGCAGCCCC AGGAAATTCG GGAGGAGCTG CTCAGCCTGG AGGAGACCAT TAAACAGCTA 
GAGGAGGTGG AGGAGGAATT TTGCCGCCTG AGACTCCTCC TGTCTCAGCT TGGGGAGAAC 
ACCGTCCCCC AGTCTGGCTG TACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF1 ( XM_010815115.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010813417.1 Bos taurus Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa48599
Clone ID Related Accession (Same CDS sequence)	XM_010815115.2 , XM_010815115.3 , XM_010823701.2
Accession Version	XM_010815115.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2967bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rho guanine nucleotide exchange factor 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010815115.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGAGA GAGGCGAGAC AGCGAGCGAC ACGGACAGCC CGGCAGTGCC CATGGAGGCA 
GAAGACGTCG CCCGAGGGGC GGCCCCGGGG CCCCCCCGAC CCGGCCTGGC GCCCGTCAGC 
ATCATTGGGG CAGAGGACGA GGATTTTGAG ATTGAGTTGA AGACGAACCC TGAGGAACAA 
AACAACCAGT TCCAGAACCT GGATCAGGTG AAGCGGCGCC CGGCCCACCT CATGGCCCTC 
CTGCAGCACG TGGCCATGCA GTTTGAGCCG GGACCCCTGC TCTGCTGCCT GCATGCGGAC 
ATGCTGGGCT CCCTGGGCCC CAAGGAGTTC AAGAAAGCCT TCATCGACTT CTACCACAGC 
TTCCTGGATA AGCACTCGAT TCTACGAGTG ATGGTCCCTC CTGCCGTGGC CTTTGAACTT 
GACCGCACGC GGCCTGACCT CTTTCCTGAG GATCTCCAGC GGCAGTTCGT GCAGGAGGTG 
GTGCAGAGCC AGCAGGTAGC CGTGGTCCGG CAGCTGGAGG ACTTCCGCTC CAAGAAGCTC 
ATGGGCATGA CACCCTGGGA GCAGGAGCTG GTCCAGCTGG GGGCCTGGGT GGCGCGGGAC 
CGTGCCAGCT ACGAGGCCCG GGAGCGGCAC CTGGCTGAGC GGCAACTGAC CCACCTGGAG 
GAGATGCAGC ACACCATCTC TAACGATGAG GAGAAGAGCG CCGCCGTGGT CAACGCCATC 
AGCATGTACA TGCGGCACCT TGGGGTGCGG ACCAAGAGTG GGGACAAGAA GTCCGGGAGG 
AACTTCTTTC GGAAAAAGAT GATAGGGAAC CGGCGGTCAG ATGAACCGAC CAAGACCAAG 
AAAGGCCTGA GCAGCTTCCT GGATGCCGCC CGCTGGAACC GGGGAGAGCC CCAGGATTTT 
CGACACCCGA AGGTCGAAGT TGATGCTGAG AAGCCAGGCC TTACAGAACG TAAGGGAGGT 
CAGGGGGCGC CTTCCCGGGA CCGGAATCTC GGAGGCCCTG GGCAGGACAC CCCCGGAGTT 
TCTCCGCACC TTCTGTCTGG GGACAGCCCT GACCGGGAGC CAGGTGTTGA CGCCCTCCTG 
GAGGTGGGGG ACCCGCCCCC ACAGGGCCCA GCCAGCCTGG ACGCCCCAGC GCCGCTGGAG 
AGCACGGAGG AGGCTGCCGA CACAGAAAGA AAGTGGAAGA GGCTGTCAGG GCGTCTGGGG 
CGCTCAGAGA GCCTGCGGGT GAGTGACCGC CGCCGGCCTT CCCGGGGTAG CCTCGGGGCT 
AAGGGCCGGG GTGGGGGCCG CTCCCGGAGC GACGTGGACA TGGACCCCAG CTCCGCCACG 
GCCGTGCTTG GCCCTACCCG ACGAGCCACC CCGGAGCCTG GAGATGAGGG AGAGCCGGGC 
CGCTCAGGAC TGGAGCTGGA ACCAGAGGAG CCGCCTGGCT GGCGAGAGCT TGTCCCCCAG 
GACATTCTGC ACAGCCTGCC CAAGAGCCAG GTGAAGCGGC AGGAGGTCAT CAGCGAGCTC 
CTGGTGACGG AGGCTGCCCA TGTGCGCATG CTCCGCGTGC TCCACGACGT CTTCTACCAG 
CCCATAGCGG ACGGGGGCTT CTACTCCCAG GAGGAGCTCC AGAACATCTT CCCCAGCCTG 
GACGAGCTCA TCGAGGTGCA TTCCCTATTC CTTGATTCCC TGATGAAGCG GAGGCAGGAC 
AGTGGCTACC TCATCAAGGA GATCGGAGAT GTGCTGCTGG CCCGGTTCGA TGGTGCCGAG 
GGCTCGTGGT TCCAGAAAAT CTCCTCCCGC TTCTGTAGCC GCCAGTCGTT CGCCTTAGAG 
CAGCTCAAAG CCAAACAGCG CAAGGAGCCC CGGTTCTGTA CCTTCGTGCA GGAGGCCGAG 
AGCCGGCCCC GGTGCCGCCG GCTGCAGCTG AAGGACATGA TCCCCACCGA GATGCAGCGT 
CTGACCAAGT ACCCACTGCT TCTGCAGAGC ATCGAGCAGA ACACAGAAGA GCCGGTGGAA 
CGGGAGAAAG TGGAGCGGGC AGCCGAGTGC TGCCGGGAAA TTCTGCAACA CGTCAACCTT 
GCCGTGCGGG ACATGGAGGA CCTGCTGCGG CTCAAAGATT ATCAGAGGCG TCTGGATTTG 
ACCCACCTGC GGCAGAGCAA CGACCCCATG CTGAGCGAGT TCAAGAACCT AGACATCACC 
AAGAAGAGGT TGATCTACGA GGGCCCACTG ACGTGGCGGG TGACGAAGGA CAAGGCTGTG 
GAGGTCCACG TGCTGCTGCT GGATGACCTG CTGCTGCTGC TTCAGCGCCA GGACGAGCGG 
TTCTTGCTTA AGTCGCACAG CCGGACGCTG ACGCCCACGC CCGATGGCAA GACCATGCTG 
CGGCCAGTGC TGCGGCTCAC CTCCGCCATG ACCCGCGAGG TGGCCACCGA TCACAAAGCC 
TTCTATGTCA TTTTTACCTG GGACCAGGAG GCCCAGATAT ACGAGCTGGT GGCGCAGACG 
GTGTCTGAGC GGAGGATCTG GTGTACCCTG ATCACCGAGA CCGCTGGATG CCTGAAGGTC 
CCCTCCCGCA CCTCCCGACC CAAACACCGG CCCGGCCCCA GCAGCACTCG TGAACCCCTG 
CTTAGCAGCT CCGAAAACGG CAATGGCAGC CGAGAGATGC CCCCGGCAGA TGGCCGGATG 
GAGAGACTCT TCAACTCCCT CCTGCCCTTC TGCAGACAAG GCCTCGAGGG CCAGCTCGCC 
GCCAAGGCCC TTCAGAAAGT GCTGACCCTG AAACAGCTCC TGTTGCCTTC GGAGGAAGAC 
AGCGGGGCAG GGCCTCCCCT CGAAGGGGAT GGGGTCCCGG GGGGCAGCCC CTTGAGCCCA 
ACGCAGCCCC AGGAAATTCG GGAGGAGCTG CTCAGCCTGG AGGAGACCAT TAAACAGCTA 
GAGGAGGTGG AGGAGGAATT TTGCCGCCTG AGACTCCTCC TGTCTCAGCT TGGGGAGAAC 
ACCGTCCCCC AGTCTGGCTG TACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010813417.1
CDS	92..3058
Translation	MGERGETASDTDSPAVPMEAEDVARGAAPGPPRPGLAPVSIIGAEDEDFEIELKTNPEEQNNQFQNLDQVKRRPAHLMALLQHVAMQFEPGPLLCCLHADMLGSLGPKEFKKAFIDFYHSFLDKHSILRVMVPPAVAFELDRTRPDLFPEDLQRQFVQEVVQSQQVAVVRQLEDFRSKKLMGMTPWEQELVQLGAWVARDRASYEARERHLAERQLTHLEEMQHTISNDEEKSAAVVNAISMYMRHLGVRTKSGDKKSGRNFFRKKMIGNRRSDEPTKTKKGLSSFLDAARWNRGEPQDFRHPKVEVDAEKPGLTERKGGQGAPSRDRNLGGPGQDTPGVSPHLLSGDSPDREPGVDALLEVGDPPPQGPASLDAPAPLESTEEAADTERKWKRLSGRLGRSESLRVSDRRRPSRGSLGAKGRGGGRSRSDVDMDPSSATAVLGPTRRATPEPGDEGEPGRSGLELEPEEPPGWRELVPQDILHSLPKSQVKRQEVISELLVTEAAHVRMLRVLHDVFYQPIADGGFYSQEELQNIFPSLDELIEVHSLFLDSLMKRRQDSGYLIKEIGDVLLARFDGAEGSWFQKISSRFCSRQSFALEQLKAKQRKEPRFCTFVQEAESRPRCRRLQLKDMIPTEMQRLTKYPLLLQSIEQNTEEPVEREKVERAAECCREILQHVNLAVRDMEDLLRLKDYQRRLDLTHLRQSNDPMLSEFKNLDITKKRLIYEGPLTWRVTKDKAVEVHVLLLDDLLLLLQRQDERFLLKSHSRTLTPTPDGKTMLRPVLRLTSAMTREVATDHKAFYVIFTWDQEAQIYELVAQTVSERRIWCTLITETAGCLKVPSRTSRPKHRPGPSSTREPLLSSSENGNGSREMPPADGRMERLFNSLLPFCRQGLEGQLAAKALQKVLTLKQLLLPSEEDSGAGPPLEGDGVPGGSPLSPTQPQEIREELLSLEETIKQLEEVEEEFCRLRLLLSQLGENTVPQSGCT

Target ORF information:

RefSeq Version	XM_010815115.3
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010815115.3

ORF Insert Sequence:

ATGGGAGAGA GAGGCGAGAC AGCGAGCGAC ACGGACAGCC CGGCAGTGCC CATGGAGGCA 
GAAGACGTCG CCCGAGGGGC GGCCCCGGGG CCCCCCCGAC CCGGCCTGGC GCCCGTCAGC 
ATCATTGGGG CAGAGGACGA GGATTTTGAG ATTGAGTTGA AGACGAACCC TGAGGAACAA 
AACAACCAGT TCCAGAACCT GGATCAGGTG AAGCGGCGCC CGGCCCACCT CATGGCCCTC 
CTGCAGCACG TGGCCATGCA GTTTGAGCCG GGACCCCTGC TCTGCTGCCT GCATGCGGAC 
ATGCTGGGCT CCCTGGGCCC CAAGGAGTTC AAGAAAGCCT TCATCGACTT CTACCACAGC 
TTCCTGGATA AGCACTCGAT TCTACGAGTG ATGGTCCCTC CTGCCGTGGC CTTTGAACTT 
GACCGCACGC GGCCTGACCT CTTTCCTGAG GATCTCCAGC GGCAGTTCGT GCAGGAGGTG 
GTGCAGAGCC AGCAGGTAGC CGTGGTCCGG CAGCTGGAGG ACTTCCGCTC CAAGAAGCTC 
ATGGGCATGA CACCCTGGGA GCAGGAGCTG GTCCAGCTGG GGGCCTGGGT GGCGCGGGAC 
CGTGCCAGCT ACGAGGCCCG GGAGCGGCAC CTGGCTGAGC GGCAACTGAC CCACCTGGAG 
GAGATGCAGC ACACCATCTC TAACGATGAG GAGAAGAGCG CCGCCGTGGT CAACGCCATC 
AGCATGTACA TGCGGCACCT TGGGGTGCGG ACCAAGAGTG GGGACAAGAA GTCCGGGAGG 
AACTTCTTTC GGAAAAAGAT GATAGGGAAC CGGCGGTCAG ATGAACCGAC CAAGACCAAG 
AAAGGCCTGA GCAGCTTCCT GGATGCCGCC CGCTGGAACC GGGGAGAGCC CCAGGATTTT 
CGACACCCGA AGGTCGAAGT TGATGCTGAG AAGCCAGGCC TTACAGAACG TAAGGGAGGT 
CAGGGGGCGC CTTCCCGGGA CCGGAATCTC GGAGGCCCTG GGCAGGACAC CCCCGGAGTT 
TCTCCGCACC TTCTGTCTGG GGACAGCCCT GACCGGGAGC CAGGTGTTGA CGCCCTCCTG 
GAGGTGGGGG ACCCGCCCCC ACAGGGCCCA GCCAGCCTGG ACGCCCCAGC GCCGCTGGAG 
AGCACGGAGG AGGCTGCCGA CACAGAAAGA AAGTGGAAGA GGCTGTCAGG GCGTCTGGGG 
CGCTCAGAGA GCCTGCGGGT GAGTGACCGC CGCCGGCCTT CCCGGGGTAG CCTCGGGGCT 
AAGGGCCGGG GTGGGGGCCG CTCCCGGAGC GACGTGGACA TGGACCCCAG CTCCGCCACG 
GCCGTGCTTG GCCCTACCCG ACGAGCCACC CCGGAGCCTG GAGATGAGGG AGAGCCGGGC 
CGCTCAGGAC TGGAGCTGGA ACCAGAGGAG CCGCCTGGCT GGCGAGAGCT TGTCCCCCAG 
GACATTCTGC ACAGCCTGCC CAAGAGCCAG GTGAAGCGGC AGGAGGTCAT CAGCGAGCTC 
CTGGTGACGG AGGCTGCCCA TGTGCGCATG CTCCGCGTGC TCCACGACGT CTTCTACCAG 
CCCATAGCGG ACGGGGGCTT CTACTCCCAG GAGGAGCTCC AGAACATCTT CCCCAGCCTG 
GACGAGCTCA TCGAGGTGCA TTCCCTATTC CTTGATTCCC TGATGAAGCG GAGGCAGGAC 
AGTGGCTACC TCATCAAGGA GATCGGAGAT GTGCTGCTGG CCCGGTTCGA TGGTGCCGAG 
GGCTCGTGGT TCCAGAAAAT CTCCTCCCGC TTCTGTAGCC GCCAGTCGTT CGCCTTAGAG 
CAGCTCAAAG CCAAACAGCG CAAGGAGCCC CGGTTCTGTA CCTTCGTGCA GGAGGCCGAG 
AGCCGGCCCC GGTGCCGCCG GCTGCAGCTG AAGGACATGA TCCCCACCGA GATGCAGCGT 
CTGACCAAGT ACCCACTGCT TCTGCAGAGC ATCGAGCAGA ACACAGAAGA GCCGGTGGAA 
CGGGAGAAAG TGGAGCGGGC AGCCGAGTGC TGCCGGGAAA TTCTGCAACA CGTCAACCTT 
GCCGTGCGGG ACATGGAGGA CCTGCTGCGG CTCAAAGATT ATCAGAGGCG TCTGGATTTG 
ACCCACCTGC GGCAGAGCAA CGACCCCATG CTGAGCGAGT TCAAGAACCT AGACATCACC 
AAGAAGAGGT TGATCTACGA GGGCCCACTG ACGTGGCGGG TGACGAAGGA CAAGGCTGTG 
GAGGTCCACG TGCTGCTGCT GGATGACCTG CTGCTGCTGC TTCAGCGCCA GGACGAGCGG 
TTCTTGCTTA AGTCGCACAG CCGGACGCTG ACGCCCACGC CCGATGGCAA GACCATGCTG 
CGGCCAGTGC TGCGGCTCAC CTCCGCCATG ACCCGCGAGG TGGCCACCGA TCACAAAGCC 
TTCTATGTCA TTTTTACCTG GGACCAGGAG GCCCAGATAT ACGAGCTGGT GGCGCAGACG 
GTGTCTGAGC GGAGGATCTG GTGTACCCTG ATCACCGAGA CCGCTGGATG CCTGAAGGTC 
CCCTCCCGCA CCTCCCGACC CAAACACCGG CCCGGCCCCA GCAGCACTCG TGAACCCCTG 
CTTAGCAGCT CCGAAAACGG CAATGGCAGC CGAGAGATGC CCCCGGCAGA TGGCCGGATG 
GAGAGACTCT TCAACTCCCT CCTGCCCTTC TGCAGACAAG GCCTCGAGGG CCAGCTCGCC 
GCCAAGGCCC TTCAGAAAGT GCTGACCCTG AAACAGCTCC TGTTGCCTTC GGAGGAAGAC 
AGCGGGGCAG GGCCTCCCCT CGAAGGGGAT GGGGTCCCGG GGGGCAGCCC CTTGAGCCCA 
ACGCAGCCCC AGGAAATTCG GGAGGAGCTG CTCAGCCTGG AGGAGACCAT TAAACAGCTA 
GAGGAGGTGG AGGAGGAATT TTGCCGCCTG AGACTCCTCC TGTCTCAGCT TGGGGAGAAC 
ACCGTCCCCC AGTCTGGCTG TACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF1 ( XM_010823701.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010822003.1 Bos taurus Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X1, mRNA.

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CloneID	OBa48599
Clone ID Related Accession (Same CDS sequence)	XM_010815115.2 , XM_010815115.3 , XM_010823701.2
Accession Version	XM_010823701.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2967bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	rho guanine nucleotide exchange factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646390.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010823701.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGAGA GAGGCGAGAC AGCGAGCGAC ACGGACAGCC CGGCAGTGCC CATGGAGGCA 
GAAGACGTCG CCCGAGGGGC GGCCCCGGGG CCCCCCCGAC CCGGCCTGGC GCCCGTCAGC 
ATCATTGGGG CAGAGGACGA GGATTTTGAG ATTGAGTTGA AGACGAACCC TGAGGAACAA 
AACAACCAGT TCCAGAACCT GGATCAGGTG AAGCGGCGCC CGGCCCACCT CATGGCCCTC 
CTGCAGCACG TGGCCATGCA GTTTGAGCCG GGACCCCTGC TCTGCTGCCT GCATGCGGAC 
ATGCTGGGCT CCCTGGGCCC CAAGGAGTTC AAGAAAGCCT TCATCGACTT CTACCACAGC 
TTCCTGGATA AGCACTCGAT TCTACGAGTG ATGGTCCCTC CTGCCGTGGC CTTTGAACTT 
GACCGCACGC GGCCTGACCT CTTTCCTGAG GATCTCCAGC GGCAGTTCGT GCAGGAGGTG 
GTGCAGAGCC AGCAGGTAGC CGTGGTCCGG CAGCTGGAGG ACTTCCGCTC CAAGAAGCTC 
ATGGGCATGA CACCCTGGGA GCAGGAGCTG GTCCAGCTGG GGGCCTGGGT GGCGCGGGAC 
CGTGCCAGCT ACGAGGCCCG GGAGCGGCAC CTGGCTGAGC GGCAACTGAC CCACCTGGAG 
GAGATGCAGC ACACCATCTC TAACGATGAG GAGAAGAGCG CCGCCGTGGT CAACGCCATC 
AGCATGTACA TGCGGCACCT TGGGGTGCGG ACCAAGAGTG GGGACAAGAA GTCCGGGAGG 
AACTTCTTTC GGAAAAAGAT GATAGGGAAC CGGCGGTCAG ATGAACCGAC CAAGACCAAG 
AAAGGCCTGA GCAGCTTCCT GGATGCCGCC CGCTGGAACC GGGGAGAGCC CCAGGATTTT 
CGACACCCGA AGGTCGAAGT TGATGCTGAG AAGCCAGGCC TTACAGAACG TAAGGGAGGT 
CAGGGGGCGC CTTCCCGGGA CCGGAATCTC GGAGGCCCTG GGCAGGACAC CCCCGGAGTT 
TCTCCGCACC TTCTGTCTGG GGACAGCCCT GACCGGGAGC CAGGTGTTGA CGCCCTCCTG 
GAGGTGGGGG ACCCGCCCCC ACAGGGCCCA GCCAGCCTGG ACGCCCCAGC GCCGCTGGAG 
AGCACGGAGG AGGCTGCCGA CACAGAAAGA AAGTGGAAGA GGCTGTCAGG GCGTCTGGGG 
CGCTCAGAGA GCCTGCGGGT GAGTGACCGC CGCCGGCCTT CCCGGGGTAG CCTCGGGGCT 
AAGGGCCGGG GTGGGGGCCG CTCCCGGAGC GACGTGGACA TGGACCCCAG CTCCGCCACG 
GCCGTGCTTG GCCCTACCCG ACGAGCCACC CCGGAGCCTG GAGATGAGGG AGAGCCGGGC 
CGCTCAGGAC TGGAGCTGGA ACCAGAGGAG CCGCCTGGCT GGCGAGAGCT TGTCCCCCAG 
GACATTCTGC ACAGCCTGCC CAAGAGCCAG GTGAAGCGGC AGGAGGTCAT CAGCGAGCTC 
CTGGTGACGG AGGCTGCCCA TGTGCGCATG CTCCGCGTGC TCCACGACGT CTTCTACCAG 
CCCATAGCGG ACGGGGGCTT CTACTCCCAG GAGGAGCTCC AGAACATCTT CCCCAGCCTG 
GACGAGCTCA TCGAGGTGCA TTCCCTATTC CTTGATTCCC TGATGAAGCG GAGGCAGGAC 
AGTGGCTACC TCATCAAGGA GATCGGAGAT GTGCTGCTGG CCCGGTTCGA TGGTGCCGAG 
GGCTCGTGGT TCCAGAAAAT CTCCTCCCGC TTCTGTAGCC GCCAGTCGTT CGCCTTAGAG 
CAGCTCAAAG CCAAACAGCG CAAGGAGCCC CGGTTCTGTA CCTTCGTGCA GGAGGCCGAG 
AGCCGGCCCC GGTGCCGCCG GCTGCAGCTG AAGGACATGA TCCCCACCGA GATGCAGCGT 
CTGACCAAGT ACCCACTGCT TCTGCAGAGC ATCGAGCAGA ACACAGAAGA GCCGGTGGAA 
CGGGAGAAAG TGGAGCGGGC AGCCGAGTGC TGCCGGGAAA TTCTGCAACA CGTCAACCTT 
GCCGTGCGGG ACATGGAGGA CCTGCTGCGG CTCAAAGATT ATCAGAGGCG TCTGGATTTG 
ACCCACCTGC GGCAGAGCAA CGACCCCATG CTGAGCGAGT TCAAGAACCT AGACATCACC 
AAGAAGAGGT TGATCTACGA GGGCCCACTG ACGTGGCGGG TGACGAAGGA CAAGGCTGTG 
GAGGTCCACG TGCTGCTGCT GGATGACCTG CTGCTGCTGC TTCAGCGCCA GGACGAGCGG 
TTCTTGCTTA AGTCGCACAG CCGGACGCTG ACGCCCACGC CCGATGGCAA GACCATGCTG 
CGGCCAGTGC TGCGGCTCAC CTCCGCCATG ACCCGCGAGG TGGCCACCGA TCACAAAGCC 
TTCTATGTCA TTTTTACCTG GGACCAGGAG GCCCAGATAT ACGAGCTGGT GGCGCAGACG 
GTGTCTGAGC GGAGGATCTG GTGTACCCTG ATCACCGAGA CCGCTGGATG CCTGAAGGTC 
CCCTCCCGCA CCTCCCGACC CAAACACCGG CCCGGCCCCA GCAGCACTCG TGAACCCCTG 
CTTAGCAGCT CCGAAAACGG CAATGGCAGC CGAGAGATGC CCCCGGCAGA TGGCCGGATG 
GAGAGACTCT TCAACTCCCT CCTGCCCTTC TGCAGACAAG GCCTCGAGGG CCAGCTCGCC 
GCCAAGGCCC TTCAGAAAGT GCTGACCCTG AAACAGCTCC TGTTGCCTTC GGAGGAAGAC 
AGCGGGGCAG GGCCTCCCCT CGAAGGGGAT GGGGTCCCGG GGGGCAGCCC CTTGAGCCCA 
ACGCAGCCCC AGGAAATTCG GGAGGAGCTG CTCAGCCTGG AGGAGACCAT TAAACAGCTA 
GAGGAGGTGG AGGAGGAATT TTGCCGCCTG AGACTCCTCC TGTCTCAGCT TGGGGAGAAC 
ACCGTCCCCC AGTCTGGCTG TACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010822003.1
CDS	139..3105
Translation	MGERGETASDTDSPAVPMEAEDVARGAAPGPPRPGLAPVSIIGAEDEDFEIELKTNPEEQNNQFQNLDQVKRRPAHLMALLQHVAMQFEPGPLLCCLHADMLGSLGPKEFKKAFIDFYHSFLDKHSILRVMVPPAVAFELDRTRPDLFPEDLQRQFVQEVVQSQQVAVVRQLEDFRSKKLMGMTPWEQELVQLGAWVARDRASYEARERHLAERQLTHLEEMQHTISNDEEKSAAVVNAISMYMRHLGVRTKSGDKKSGRNFFRKKMIGNRRSDEPTKTKKGLSSFLDAARWNRGEPQDFRHPKVEVDAEKPGLTERKGGQGAPSRDRNLGGPGQDTPGVSPHLLSGDSPDREPGVDALLEVGDPPPQGPASLDAPAPLESTEEAADTERKWKRLSGRLGRSESLRVSDRRRPSRGSLGAKGRGGGRSRSDVDMDPSSATAVLGPTRRATPEPGDEGEPGRSGLELEPEEPPGWRELVPQDILHSLPKSQVKRQEVISELLVTEAAHVRMLRVLHDVFYQPIADGGFYSQEELQNIFPSLDELIEVHSLFLDSLMKRRQDSGYLIKEIGDVLLARFDGAEGSWFQKISSRFCSRQSFALEQLKAKQRKEPRFCTFVQEAESRPRCRRLQLKDMIPTEMQRLTKYPLLLQSIEQNTEEPVEREKVERAAECCREILQHVNLAVRDMEDLLRLKDYQRRLDLTHLRQSNDPMLSEFKNLDITKKRLIYEGPLTWRVTKDKAVEVHVLLLDDLLLLLQRQDERFLLKSHSRTLTPTPDGKTMLRPVLRLTSAMTREVATDHKAFYVIFTWDQEAQIYELVAQTVSERRIWCTLITETAGCLKVPSRTSRPKHRPGPSSTREPLLSSSENGNGSREMPPADGRMERLFNSLLPFCRQGLEGQLAAKALQKVLTLKQLLLPSEEDSGAGPPLEGDGVPGGSPLSPTQPQEIREELLSLEETIKQLEEVEEEFCRLRLLLSQLGENTVPQSGCT

Target ORF information:

RefSeq Version	XM_010823701.2
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010823701.2

ORF Insert Sequence:

ATGGGAGAGA GAGGCGAGAC AGCGAGCGAC ACGGACAGCC CGGCAGTGCC CATGGAGGCA 
GAAGACGTCG CCCGAGGGGC GGCCCCGGGG CCCCCCCGAC CCGGCCTGGC GCCCGTCAGC 
ATCATTGGGG CAGAGGACGA GGATTTTGAG ATTGAGTTGA AGACGAACCC TGAGGAACAA 
AACAACCAGT TCCAGAACCT GGATCAGGTG AAGCGGCGCC CGGCCCACCT CATGGCCCTC 
CTGCAGCACG TGGCCATGCA GTTTGAGCCG GGACCCCTGC TCTGCTGCCT GCATGCGGAC 
ATGCTGGGCT CCCTGGGCCC CAAGGAGTTC AAGAAAGCCT TCATCGACTT CTACCACAGC 
TTCCTGGATA AGCACTCGAT TCTACGAGTG ATGGTCCCTC CTGCCGTGGC CTTTGAACTT 
GACCGCACGC GGCCTGACCT CTTTCCTGAG GATCTCCAGC GGCAGTTCGT GCAGGAGGTG 
GTGCAGAGCC AGCAGGTAGC CGTGGTCCGG CAGCTGGAGG ACTTCCGCTC CAAGAAGCTC 
ATGGGCATGA CACCCTGGGA GCAGGAGCTG GTCCAGCTGG GGGCCTGGGT GGCGCGGGAC 
CGTGCCAGCT ACGAGGCCCG GGAGCGGCAC CTGGCTGAGC GGCAACTGAC CCACCTGGAG 
GAGATGCAGC ACACCATCTC TAACGATGAG GAGAAGAGCG CCGCCGTGGT CAACGCCATC 
AGCATGTACA TGCGGCACCT TGGGGTGCGG ACCAAGAGTG GGGACAAGAA GTCCGGGAGG 
AACTTCTTTC GGAAAAAGAT GATAGGGAAC CGGCGGTCAG ATGAACCGAC CAAGACCAAG 
AAAGGCCTGA GCAGCTTCCT GGATGCCGCC CGCTGGAACC GGGGAGAGCC CCAGGATTTT 
CGACACCCGA AGGTCGAAGT TGATGCTGAG AAGCCAGGCC TTACAGAACG TAAGGGAGGT 
CAGGGGGCGC CTTCCCGGGA CCGGAATCTC GGAGGCCCTG GGCAGGACAC CCCCGGAGTT 
TCTCCGCACC TTCTGTCTGG GGACAGCCCT GACCGGGAGC CAGGTGTTGA CGCCCTCCTG 
GAGGTGGGGG ACCCGCCCCC ACAGGGCCCA GCCAGCCTGG ACGCCCCAGC GCCGCTGGAG 
AGCACGGAGG AGGCTGCCGA CACAGAAAGA AAGTGGAAGA GGCTGTCAGG GCGTCTGGGG 
CGCTCAGAGA GCCTGCGGGT GAGTGACCGC CGCCGGCCTT CCCGGGGTAG CCTCGGGGCT 
AAGGGCCGGG GTGGGGGCCG CTCCCGGAGC GACGTGGACA TGGACCCCAG CTCCGCCACG 
GCCGTGCTTG GCCCTACCCG ACGAGCCACC CCGGAGCCTG GAGATGAGGG AGAGCCGGGC 
CGCTCAGGAC TGGAGCTGGA ACCAGAGGAG CCGCCTGGCT GGCGAGAGCT TGTCCCCCAG 
GACATTCTGC ACAGCCTGCC CAAGAGCCAG GTGAAGCGGC AGGAGGTCAT CAGCGAGCTC 
CTGGTGACGG AGGCTGCCCA TGTGCGCATG CTCCGCGTGC TCCACGACGT CTTCTACCAG 
CCCATAGCGG ACGGGGGCTT CTACTCCCAG GAGGAGCTCC AGAACATCTT CCCCAGCCTG 
GACGAGCTCA TCGAGGTGCA TTCCCTATTC CTTGATTCCC TGATGAAGCG GAGGCAGGAC 
AGTGGCTACC TCATCAAGGA GATCGGAGAT GTGCTGCTGG CCCGGTTCGA TGGTGCCGAG 
GGCTCGTGGT TCCAGAAAAT CTCCTCCCGC TTCTGTAGCC GCCAGTCGTT CGCCTTAGAG 
CAGCTCAAAG CCAAACAGCG CAAGGAGCCC CGGTTCTGTA CCTTCGTGCA GGAGGCCGAG 
AGCCGGCCCC GGTGCCGCCG GCTGCAGCTG AAGGACATGA TCCCCACCGA GATGCAGCGT 
CTGACCAAGT ACCCACTGCT TCTGCAGAGC ATCGAGCAGA ACACAGAAGA GCCGGTGGAA 
CGGGAGAAAG TGGAGCGGGC AGCCGAGTGC TGCCGGGAAA TTCTGCAACA CGTCAACCTT 
GCCGTGCGGG ACATGGAGGA CCTGCTGCGG CTCAAAGATT ATCAGAGGCG TCTGGATTTG 
ACCCACCTGC GGCAGAGCAA CGACCCCATG CTGAGCGAGT TCAAGAACCT AGACATCACC 
AAGAAGAGGT TGATCTACGA GGGCCCACTG ACGTGGCGGG TGACGAAGGA CAAGGCTGTG 
GAGGTCCACG TGCTGCTGCT GGATGACCTG CTGCTGCTGC TTCAGCGCCA GGACGAGCGG 
TTCTTGCTTA AGTCGCACAG CCGGACGCTG ACGCCCACGC CCGATGGCAA GACCATGCTG 
CGGCCAGTGC TGCGGCTCAC CTCCGCCATG ACCCGCGAGG TGGCCACCGA TCACAAAGCC 
TTCTATGTCA TTTTTACCTG GGACCAGGAG GCCCAGATAT ACGAGCTGGT GGCGCAGACG 
GTGTCTGAGC GGAGGATCTG GTGTACCCTG ATCACCGAGA CCGCTGGATG CCTGAAGGTC 
CCCTCCCGCA CCTCCCGACC CAAACACCGG CCCGGCCCCA GCAGCACTCG TGAACCCCTG 
CTTAGCAGCT CCGAAAACGG CAATGGCAGC CGAGAGATGC CCCCGGCAGA TGGCCGGATG 
GAGAGACTCT TCAACTCCCT CCTGCCCTTC TGCAGACAAG GCCTCGAGGG CCAGCTCGCC 
GCCAAGGCCC TTCAGAAAGT GCTGACCCTG AAACAGCTCC TGTTGCCTTC GGAGGAAGAC 
AGCGGGGCAG GGCCTCCCCT CGAAGGGGAT GGGGTCCCGG GGGGCAGCCC CTTGAGCCCA 
ACGCAGCCCC AGGAAATTCG GGAGGAGCTG CTCAGCCTGG AGGAGACCAT TAAACAGCTA 
GAGGAGGTGG AGGAGGAATT TTGCCGCCTG AGACTCCTCC TGTCTCAGCT TGGGGAGAAC 
ACCGTCCCCC AGTCTGGCTG TACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF1 ( XM_010815116.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010813418.1 Bos taurus Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa48600
Clone ID Related Accession (Same CDS sequence)	XM_010815116.2 , XM_010823702.2
Accession Version	XM_010815116.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2955bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	rho guanine nucleotide exchange factor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104466.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010815116.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGAGA GAGGCGAGAC AGCGAGCGAC ACGGACAGCC CGGCAGTGCC CATGGAGGCA 
GAAGACGTCG CCCGAGGGGC GGCCCCGGGG CCCCCCCGAC CCGGCCTGGC GCCCGTCAGC 
ATCATTGGGG CAGAGGACGA GGATTTTGAG ATTGAGTTGA AGACGAACCC TGAGGAACAA 
AACAACCAGT TCCAGAACCT GGATCAGGTG AAGCGGCGCC CGGCCCACCT CATGGCCCTC 
CTGCAGCACG TGGCCATGCA GTTTGAGCCG GGACCCCTGC TCTGCTGCCT GCATGCGGAC 
ATGCTGGGCT CCCTGGGCCC CAAGGAGTTC AAGAAAGCCT TCATCGACTT CTACCACAGC 
TTCCTGGATA AGCACTCGAT TCTACGAGTG ATGGTCCCTC CTGCCGTGGC CTTTGAACTT 
GACCGCACGC GGCCTGACCT CTTTCCTGAG GATCTCCAGC GGCAGTTCGT GCAGGAGGTG 
GTGCAGAGCC AGCAGGTAGC CGTGGTCCGG CAGCTGGAGG ACTTCCGCTC CAAGAAGCTC 
ATGGGCATGA CACCCTGGGA GCAGGAGCTG GTCCAGCTGG GGGCCTGGGT GGCGCGGGAC 
CGTGCCAGCT ACGAGGCCCG GGAGCGGCAC CTGGCTGAGC GGCAACTGAC CCACCTGGAG 
GAGATGCAGC ACACCATCTC TAACGATGAG GAGAAGAGCG CCGCCGTGGT CAACGCCATC 
AGCATGTACA TGCGGCACCT TGGGGTGCGG ACCAAGAGTG GGGACAAGAA GTCCGGGAGG 
AACTTCTTTC GGAAAAAGAT GATAGGGAAC CGGCGGTCAG ATGAACCGAC CAAGACCAAG 
AAAGGCCTGA GCAGCTTCCT GGATGCCGCC CGCTGGAACC GGGGAGAGCC CCAGGATTTT 
CGACACCCGA AGGTCGAAGT TGATGCTGAG AAGCCAGGCC TTACAGAACG TAAGGGAGGT 
CAGGGGGCGC CTTCCCGGGA CCGGAATCTC GGAGGCCCTG GGCAGGACAC CCCCGGAGTT 
TCTCCGCACC TTCTGTCTGG GGACAGCCCT GACCGGGAGC CAGGTGTTGA CGCCCTCCTG 
GAGGTGGGGG ACCCGCCCCC ACAGGGCCCA GCCAGCCTGG ACGCCCCAGC GCCGCTGGAG 
AGCACGGAGG AGGCTGCCGA CACAGAAAGG CTGTCAGGGC GTCTGGGGCG CTCAGAGAGC 
CTGCGGGTGA GTGACCGCCG CCGGCCTTCC CGGGGTAGCC TCGGGGCTAA GGGCCGGGGT 
GGGGGCCGCT CCCGGAGCGA CGTGGACATG GACCCCAGCT CCGCCACGGC CGTGCTTGGC 
CCTACCCGAC GAGCCACCCC GGAGCCTGGA GATGAGGGAG AGCCGGGCCG CTCAGGACTG 
GAGCTGGAAC CAGAGGAGCC GCCTGGCTGG CGAGAGCTTG TCCCCCAGGA CATTCTGCAC 
AGCCTGCCCA AGAGCCAGGT GAAGCGGCAG GAGGTCATCA GCGAGCTCCT GGTGACGGAG 
GCTGCCCATG TGCGCATGCT CCGCGTGCTC CACGACGTCT TCTACCAGCC CATAGCGGAC 
GGGGGCTTCT ACTCCCAGGA GGAGCTCCAG AACATCTTCC CCAGCCTGGA CGAGCTCATC 
GAGGTGCATT CCCTATTCCT TGATTCCCTG ATGAAGCGGA GGCAGGACAG TGGCTACCTC 
ATCAAGGAGA TCGGAGATGT GCTGCTGGCC CGGTTCGATG GTGCCGAGGG CTCGTGGTTC 
CAGAAAATCT CCTCCCGCTT CTGTAGCCGC CAGTCGTTCG CCTTAGAGCA GCTCAAAGCC 
AAACAGCGCA AGGAGCCCCG GTTCTGTACC TTCGTGCAGG AGGCCGAGAG CCGGCCCCGG 
TGCCGCCGGC TGCAGCTGAA GGACATGATC CCCACCGAGA TGCAGCGTCT GACCAAGTAC 
CCACTGCTTC TGCAGAGCAT CGAGCAGAAC ACAGAAGAGC CGGTGGAACG GGAGAAAGTG 
GAGCGGGCAG CCGAGTGCTG CCGGGAAATT CTGCAACACG TCAACCTTGC CGTGCGGGAC 
ATGGAGGACC TGCTGCGGCT CAAAGATTAT CAGAGGCGTC TGGATTTGAC CCACCTGCGG 
CAGAGCAACG ACCCCATGCT GAGCGAGTTC AAGAACCTAG ACATCACCAA GAAGAGGTTG 
ATCTACGAGG GCCCACTGAC GTGGCGGGTG ACGAAGGACA AGGCTGTGGA GGTCCACGTG 
CTGCTGCTGG ATGACCTGCT GCTGCTGCTT CAGCGCCAGG ACGAGCGGTT CTTGCTTAAG 
TCGCACAGCC GGACGCTGAC GCCCACGCCC GATGGCAAGA CCATGCTGCG GCCAGTGCTG 
CGGCTCACCT CCGCCATGAC CCGCGAGGTG GCCACCGATC ACAAAGCCTT CTATGTCATT 
TTTACCTGGG ACCAGGAGGC CCAGATATAC GAGCTGGTGG CGCAGACGGT GTCTGAGCGG 
AGGATCTGGT GTACCCTGAT CACCGAGACC GCTGGATGCC TGAAGGTCCC CTCCCGCACC 
TCCCGACCCA AACACCGGCC CGGCCCCAGC AGCACTCGTG AACCCCTGCT TAGCAGCTCC 
GAAAACGGCA ATGGCAGCCG AGAGATGCCC CCGGCAGATG GCCGGATGGA GAGACTCTTC 
AACTCCCTCC TGCCCTTCTG CAGACAAGGC CTCGAGGGCC AGCTCGCCGC CAAGGCCCTT 
CAGAAAGTGC TGACCCTGAA ACAGCTCCTG TTGCCTTCGG AGGAAGACAG CGGGGCAGGG 
CCTCCCCTCG AAGGGGATGG GGTCCCGGGG GGCAGCCCCT TGAGCCCAAC GCAGCCCCAG 
GAAATTCGGG AGGAGCTGCT CAGCCTGGAG GAGACCATTA AACAGCTAGA GGAGGTGGAG 
GAGGAATTTT GCCGCCTGAG ACTCCTCCTG TCTCAGCTTG GGGAGAACAC CGTCCCCCAG 
TCTGGCTGTA CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010813418.1
CDS	139..3093
Translation	MGERGETASDTDSPAVPMEAEDVARGAAPGPPRPGLAPVSIIGAEDEDFEIELKTNPEEQNNQFQNLDQVKRRPAHLMALLQHVAMQFEPGPLLCCLHADMLGSLGPKEFKKAFIDFYHSFLDKHSILRVMVPPAVAFELDRTRPDLFPEDLQRQFVQEVVQSQQVAVVRQLEDFRSKKLMGMTPWEQELVQLGAWVARDRASYEARERHLAERQLTHLEEMQHTISNDEEKSAAVVNAISMYMRHLGVRTKSGDKKSGRNFFRKKMIGNRRSDEPTKTKKGLSSFLDAARWNRGEPQDFRHPKVEVDAEKPGLTERKGGQGAPSRDRNLGGPGQDTPGVSPHLLSGDSPDREPGVDALLEVGDPPPQGPASLDAPAPLESTEEAADTERLSGRLGRSESLRVSDRRRPSRGSLGAKGRGGGRSRSDVDMDPSSATAVLGPTRRATPEPGDEGEPGRSGLELEPEEPPGWRELVPQDILHSLPKSQVKRQEVISELLVTEAAHVRMLRVLHDVFYQPIADGGFYSQEELQNIFPSLDELIEVHSLFLDSLMKRRQDSGYLIKEIGDVLLARFDGAEGSWFQKISSRFCSRQSFALEQLKAKQRKEPRFCTFVQEAESRPRCRRLQLKDMIPTEMQRLTKYPLLLQSIEQNTEEPVEREKVERAAECCREILQHVNLAVRDMEDLLRLKDYQRRLDLTHLRQSNDPMLSEFKNLDITKKRLIYEGPLTWRVTKDKAVEVHVLLLDDLLLLLQRQDERFLLKSHSRTLTPTPDGKTMLRPVLRLTSAMTREVATDHKAFYVIFTWDQEAQIYELVAQTVSERRIWCTLITETAGCLKVPSRTSRPKHRPGPSSTREPLLSSSENGNGSREMPPADGRMERLFNSLLPFCRQGLEGQLAAKALQKVLTLKQLLLPSEEDSGAGPPLEGDGVPGGSPLSPTQPQEIREELLSLEETIKQLEEVEEEFCRLRLLLSQLGENTVPQSGCT

Target ORF information:

RefSeq Version	XM_010815116.2
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010815116.2

ORF Insert Sequence:

ATGGGAGAGA GAGGCGAGAC AGCGAGCGAC ACGGACAGCC CGGCAGTGCC CATGGAGGCA 
GAAGACGTCG CCCGAGGGGC GGCCCCGGGG CCCCCCCGAC CCGGCCTGGC GCCCGTCAGC 
ATCATTGGGG CAGAGGACGA GGATTTTGAG ATTGAGTTGA AGACGAACCC TGAGGAACAA 
AACAACCAGT TCCAGAACCT GGATCAGGTG AAGCGGCGCC CGGCCCACCT CATGGCCCTC 
CTGCAGCACG TGGCCATGCA GTTTGAGCCG GGACCCCTGC TCTGCTGCCT GCATGCGGAC 
ATGCTGGGCT CCCTGGGCCC CAAGGAGTTC AAGAAAGCCT TCATCGACTT CTACCACAGC 
TTCCTGGATA AGCACTCGAT TCTACGAGTG ATGGTCCCTC CTGCCGTGGC CTTTGAACTT 
GACCGCACGC GGCCTGACCT CTTTCCTGAG GATCTCCAGC GGCAGTTCGT GCAGGAGGTG 
GTGCAGAGCC AGCAGGTAGC CGTGGTCCGG CAGCTGGAGG ACTTCCGCTC CAAGAAGCTC 
ATGGGCATGA CACCCTGGGA GCAGGAGCTG GTCCAGCTGG GGGCCTGGGT GGCGCGGGAC 
CGTGCCAGCT ACGAGGCCCG GGAGCGGCAC CTGGCTGAGC GGCAACTGAC CCACCTGGAG 
GAGATGCAGC ACACCATCTC TAACGATGAG GAGAAGAGCG CCGCCGTGGT CAACGCCATC 
AGCATGTACA TGCGGCACCT TGGGGTGCGG ACCAAGAGTG GGGACAAGAA GTCCGGGAGG 
AACTTCTTTC GGAAAAAGAT GATAGGGAAC CGGCGGTCAG ATGAACCGAC CAAGACCAAG 
AAAGGCCTGA GCAGCTTCCT GGATGCCGCC CGCTGGAACC GGGGAGAGCC CCAGGATTTT 
CGACACCCGA AGGTCGAAGT TGATGCTGAG AAGCCAGGCC TTACAGAACG TAAGGGAGGT 
CAGGGGGCGC CTTCCCGGGA CCGGAATCTC GGAGGCCCTG GGCAGGACAC CCCCGGAGTT 
TCTCCGCACC TTCTGTCTGG GGACAGCCCT GACCGGGAGC CAGGTGTTGA CGCCCTCCTG 
GAGGTGGGGG ACCCGCCCCC ACAGGGCCCA GCCAGCCTGG ACGCCCCAGC GCCGCTGGAG 
AGCACGGAGG AGGCTGCCGA CACAGAAAGG CTGTCAGGGC GTCTGGGGCG CTCAGAGAGC 
CTGCGGGTGA GTGACCGCCG CCGGCCTTCC CGGGGTAGCC TCGGGGCTAA GGGCCGGGGT 
GGGGGCCGCT CCCGGAGCGA CGTGGACATG GACCCCAGCT CCGCCACGGC CGTGCTTGGC 
CCTACCCGAC GAGCCACCCC GGAGCCTGGA GATGAGGGAG AGCCGGGCCG CTCAGGACTG 
GAGCTGGAAC CAGAGGAGCC GCCTGGCTGG CGAGAGCTTG TCCCCCAGGA CATTCTGCAC 
AGCCTGCCCA AGAGCCAGGT GAAGCGGCAG GAGGTCATCA GCGAGCTCCT GGTGACGGAG 
GCTGCCCATG TGCGCATGCT CCGCGTGCTC CACGACGTCT TCTACCAGCC CATAGCGGAC 
GGGGGCTTCT ACTCCCAGGA GGAGCTCCAG AACATCTTCC CCAGCCTGGA CGAGCTCATC 
GAGGTGCATT CCCTATTCCT TGATTCCCTG ATGAAGCGGA GGCAGGACAG TGGCTACCTC 
ATCAAGGAGA TCGGAGATGT GCTGCTGGCC CGGTTCGATG GTGCCGAGGG CTCGTGGTTC 
CAGAAAATCT CCTCCCGCTT CTGTAGCCGC CAGTCGTTCG CCTTAGAGCA GCTCAAAGCC 
AAACAGCGCA AGGAGCCCCG GTTCTGTACC TTCGTGCAGG AGGCCGAGAG CCGGCCCCGG 
TGCCGCCGGC TGCAGCTGAA GGACATGATC CCCACCGAGA TGCAGCGTCT GACCAAGTAC 
CCACTGCTTC TGCAGAGCAT CGAGCAGAAC ACAGAAGAGC CGGTGGAACG GGAGAAAGTG 
GAGCGGGCAG CCGAGTGCTG CCGGGAAATT CTGCAACACG TCAACCTTGC CGTGCGGGAC 
ATGGAGGACC TGCTGCGGCT CAAAGATTAT CAGAGGCGTC TGGATTTGAC CCACCTGCGG 
CAGAGCAACG ACCCCATGCT GAGCGAGTTC AAGAACCTAG ACATCACCAA GAAGAGGTTG 
ATCTACGAGG GCCCACTGAC GTGGCGGGTG ACGAAGGACA AGGCTGTGGA GGTCCACGTG 
CTGCTGCTGG ATGACCTGCT GCTGCTGCTT CAGCGCCAGG ACGAGCGGTT CTTGCTTAAG 
TCGCACAGCC GGACGCTGAC GCCCACGCCC GATGGCAAGA CCATGCTGCG GCCAGTGCTG 
CGGCTCACCT CCGCCATGAC CCGCGAGGTG GCCACCGATC ACAAAGCCTT CTATGTCATT 
TTTACCTGGG ACCAGGAGGC CCAGATATAC GAGCTGGTGG CGCAGACGGT GTCTGAGCGG 
AGGATCTGGT GTACCCTGAT CACCGAGACC GCTGGATGCC TGAAGGTCCC CTCCCGCACC 
TCCCGACCCA AACACCGGCC CGGCCCCAGC AGCACTCGTG AACCCCTGCT TAGCAGCTCC 
GAAAACGGCA ATGGCAGCCG AGAGATGCCC CCGGCAGATG GCCGGATGGA GAGACTCTTC 
AACTCCCTCC TGCCCTTCTG CAGACAAGGC CTCGAGGGCC AGCTCGCCGC CAAGGCCCTT 
CAGAAAGTGC TGACCCTGAA ACAGCTCCTG TTGCCTTCGG AGGAAGACAG CGGGGCAGGG 
CCTCCCCTCG AAGGGGATGG GGTCCCGGGG GGCAGCCCCT TGAGCCCAAC GCAGCCCCAG 
GAAATTCGGG AGGAGCTGCT CAGCCTGGAG GAGACCATTA AACAGCTAGA GGAGGTGGAG 
GAGGAATTTT GCCGCCTGAG ACTCCTCCTG TCTCAGCTTG GGGAGAACAC CGTCCCCCAG 
TCTGGCTGTA CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF1 ( XM_010823702.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010822004.1 Bos taurus Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa48600
Clone ID Related Accession (Same CDS sequence)	XM_010815116.2 , XM_010823702.2
Accession Version	XM_010823702.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2955bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	rho guanine nucleotide exchange factor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646390.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010823702.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGAGA GAGGCGAGAC AGCGAGCGAC ACGGACAGCC CGGCAGTGCC CATGGAGGCA 
GAAGACGTCG CCCGAGGGGC GGCCCCGGGG CCCCCCCGAC CCGGCCTGGC GCCCGTCAGC 
ATCATTGGGG CAGAGGACGA GGATTTTGAG ATTGAGTTGA AGACGAACCC TGAGGAACAA 
AACAACCAGT TCCAGAACCT GGATCAGGTG AAGCGGCGCC CGGCCCACCT CATGGCCCTC 
CTGCAGCACG TGGCCATGCA GTTTGAGCCG GGACCCCTGC TCTGCTGCCT GCATGCGGAC 
ATGCTGGGCT CCCTGGGCCC CAAGGAGTTC AAGAAAGCCT TCATCGACTT CTACCACAGC 
TTCCTGGATA AGCACTCGAT TCTACGAGTG ATGGTCCCTC CTGCCGTGGC CTTTGAACTT 
GACCGCACGC GGCCTGACCT CTTTCCTGAG GATCTCCAGC GGCAGTTCGT GCAGGAGGTG 
GTGCAGAGCC AGCAGGTAGC CGTGGTCCGG CAGCTGGAGG ACTTCCGCTC CAAGAAGCTC 
ATGGGCATGA CACCCTGGGA GCAGGAGCTG GTCCAGCTGG GGGCCTGGGT GGCGCGGGAC 
CGTGCCAGCT ACGAGGCCCG GGAGCGGCAC CTGGCTGAGC GGCAACTGAC CCACCTGGAG 
GAGATGCAGC ACACCATCTC TAACGATGAG GAGAAGAGCG CCGCCGTGGT CAACGCCATC 
AGCATGTACA TGCGGCACCT TGGGGTGCGG ACCAAGAGTG GGGACAAGAA GTCCGGGAGG 
AACTTCTTTC GGAAAAAGAT GATAGGGAAC CGGCGGTCAG ATGAACCGAC CAAGACCAAG 
AAAGGCCTGA GCAGCTTCCT GGATGCCGCC CGCTGGAACC GGGGAGAGCC CCAGGATTTT 
CGACACCCGA AGGTCGAAGT TGATGCTGAG AAGCCAGGCC TTACAGAACG TAAGGGAGGT 
CAGGGGGCGC CTTCCCGGGA CCGGAATCTC GGAGGCCCTG GGCAGGACAC CCCCGGAGTT 
TCTCCGCACC TTCTGTCTGG GGACAGCCCT GACCGGGAGC CAGGTGTTGA CGCCCTCCTG 
GAGGTGGGGG ACCCGCCCCC ACAGGGCCCA GCCAGCCTGG ACGCCCCAGC GCCGCTGGAG 
AGCACGGAGG AGGCTGCCGA CACAGAAAGG CTGTCAGGGC GTCTGGGGCG CTCAGAGAGC 
CTGCGGGTGA GTGACCGCCG CCGGCCTTCC CGGGGTAGCC TCGGGGCTAA GGGCCGGGGT 
GGGGGCCGCT CCCGGAGCGA CGTGGACATG GACCCCAGCT CCGCCACGGC CGTGCTTGGC 
CCTACCCGAC GAGCCACCCC GGAGCCTGGA GATGAGGGAG AGCCGGGCCG CTCAGGACTG 
GAGCTGGAAC CAGAGGAGCC GCCTGGCTGG CGAGAGCTTG TCCCCCAGGA CATTCTGCAC 
AGCCTGCCCA AGAGCCAGGT GAAGCGGCAG GAGGTCATCA GCGAGCTCCT GGTGACGGAG 
GCTGCCCATG TGCGCATGCT CCGCGTGCTC CACGACGTCT TCTACCAGCC CATAGCGGAC 
GGGGGCTTCT ACTCCCAGGA GGAGCTCCAG AACATCTTCC CCAGCCTGGA CGAGCTCATC 
GAGGTGCATT CCCTATTCCT TGATTCCCTG ATGAAGCGGA GGCAGGACAG TGGCTACCTC 
ATCAAGGAGA TCGGAGATGT GCTGCTGGCC CGGTTCGATG GTGCCGAGGG CTCGTGGTTC 
CAGAAAATCT CCTCCCGCTT CTGTAGCCGC CAGTCGTTCG CCTTAGAGCA GCTCAAAGCC 
AAACAGCGCA AGGAGCCCCG GTTCTGTACC TTCGTGCAGG AGGCCGAGAG CCGGCCCCGG 
TGCCGCCGGC TGCAGCTGAA GGACATGATC CCCACCGAGA TGCAGCGTCT GACCAAGTAC 
CCACTGCTTC TGCAGAGCAT CGAGCAGAAC ACAGAAGAGC CGGTGGAACG GGAGAAAGTG 
GAGCGGGCAG CCGAGTGCTG CCGGGAAATT CTGCAACACG TCAACCTTGC CGTGCGGGAC 
ATGGAGGACC TGCTGCGGCT CAAAGATTAT CAGAGGCGTC TGGATTTGAC CCACCTGCGG 
CAGAGCAACG ACCCCATGCT GAGCGAGTTC AAGAACCTAG ACATCACCAA GAAGAGGTTG 
ATCTACGAGG GCCCACTGAC GTGGCGGGTG ACGAAGGACA AGGCTGTGGA GGTCCACGTG 
CTGCTGCTGG ATGACCTGCT GCTGCTGCTT CAGCGCCAGG ACGAGCGGTT CTTGCTTAAG 
TCGCACAGCC GGACGCTGAC GCCCACGCCC GATGGCAAGA CCATGCTGCG GCCAGTGCTG 
CGGCTCACCT CCGCCATGAC CCGCGAGGTG GCCACCGATC ACAAAGCCTT CTATGTCATT 
TTTACCTGGG ACCAGGAGGC CCAGATATAC GAGCTGGTGG CGCAGACGGT GTCTGAGCGG 
AGGATCTGGT GTACCCTGAT CACCGAGACC GCTGGATGCC TGAAGGTCCC CTCCCGCACC 
TCCCGACCCA AACACCGGCC CGGCCCCAGC AGCACTCGTG AACCCCTGCT TAGCAGCTCC 
GAAAACGGCA ATGGCAGCCG AGAGATGCCC CCGGCAGATG GCCGGATGGA GAGACTCTTC 
AACTCCCTCC TGCCCTTCTG CAGACAAGGC CTCGAGGGCC AGCTCGCCGC CAAGGCCCTT 
CAGAAAGTGC TGACCCTGAA ACAGCTCCTG TTGCCTTCGG AGGAAGACAG CGGGGCAGGG 
CCTCCCCTCG AAGGGGATGG GGTCCCGGGG GGCAGCCCCT TGAGCCCAAC GCAGCCCCAG 
GAAATTCGGG AGGAGCTGCT CAGCCTGGAG GAGACCATTA AACAGCTAGA GGAGGTGGAG 
GAGGAATTTT GCCGCCTGAG ACTCCTCCTG TCTCAGCTTG GGGAGAACAC CGTCCCCCAG 
TCTGGCTGTA CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010822004.1
CDS	139..3093
Translation	MGERGETASDTDSPAVPMEAEDVARGAAPGPPRPGLAPVSIIGAEDEDFEIELKTNPEEQNNQFQNLDQVKRRPAHLMALLQHVAMQFEPGPLLCCLHADMLGSLGPKEFKKAFIDFYHSFLDKHSILRVMVPPAVAFELDRTRPDLFPEDLQRQFVQEVVQSQQVAVVRQLEDFRSKKLMGMTPWEQELVQLGAWVARDRASYEARERHLAERQLTHLEEMQHTISNDEEKSAAVVNAISMYMRHLGVRTKSGDKKSGRNFFRKKMIGNRRSDEPTKTKKGLSSFLDAARWNRGEPQDFRHPKVEVDAEKPGLTERKGGQGAPSRDRNLGGPGQDTPGVSPHLLSGDSPDREPGVDALLEVGDPPPQGPASLDAPAPLESTEEAADTERLSGRLGRSESLRVSDRRRPSRGSLGAKGRGGGRSRSDVDMDPSSATAVLGPTRRATPEPGDEGEPGRSGLELEPEEPPGWRELVPQDILHSLPKSQVKRQEVISELLVTEAAHVRMLRVLHDVFYQPIADGGFYSQEELQNIFPSLDELIEVHSLFLDSLMKRRQDSGYLIKEIGDVLLARFDGAEGSWFQKISSRFCSRQSFALEQLKAKQRKEPRFCTFVQEAESRPRCRRLQLKDMIPTEMQRLTKYPLLLQSIEQNTEEPVEREKVERAAECCREILQHVNLAVRDMEDLLRLKDYQRRLDLTHLRQSNDPMLSEFKNLDITKKRLIYEGPLTWRVTKDKAVEVHVLLLDDLLLLLQRQDERFLLKSHSRTLTPTPDGKTMLRPVLRLTSAMTREVATDHKAFYVIFTWDQEAQIYELVAQTVSERRIWCTLITETAGCLKVPSRTSRPKHRPGPSSTREPLLSSSENGNGSREMPPADGRMERLFNSLLPFCRQGLEGQLAAKALQKVLTLKQLLLPSEEDSGAGPPLEGDGVPGGSPLSPTQPQEIREELLSLEETIKQLEEVEEEFCRLRLLLSQLGENTVPQSGCT

Target ORF information:

RefSeq Version	XM_010823702.2
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010823702.2

ORF Insert Sequence:

ATGGGAGAGA GAGGCGAGAC AGCGAGCGAC ACGGACAGCC CGGCAGTGCC CATGGAGGCA 
GAAGACGTCG CCCGAGGGGC GGCCCCGGGG CCCCCCCGAC CCGGCCTGGC GCCCGTCAGC 
ATCATTGGGG CAGAGGACGA GGATTTTGAG ATTGAGTTGA AGACGAACCC TGAGGAACAA 
AACAACCAGT TCCAGAACCT GGATCAGGTG AAGCGGCGCC CGGCCCACCT CATGGCCCTC 
CTGCAGCACG TGGCCATGCA GTTTGAGCCG GGACCCCTGC TCTGCTGCCT GCATGCGGAC 
ATGCTGGGCT CCCTGGGCCC CAAGGAGTTC AAGAAAGCCT TCATCGACTT CTACCACAGC 
TTCCTGGATA AGCACTCGAT TCTACGAGTG ATGGTCCCTC CTGCCGTGGC CTTTGAACTT 
GACCGCACGC GGCCTGACCT CTTTCCTGAG GATCTCCAGC GGCAGTTCGT GCAGGAGGTG 
GTGCAGAGCC AGCAGGTAGC CGTGGTCCGG CAGCTGGAGG ACTTCCGCTC CAAGAAGCTC 
ATGGGCATGA CACCCTGGGA GCAGGAGCTG GTCCAGCTGG GGGCCTGGGT GGCGCGGGAC 
CGTGCCAGCT ACGAGGCCCG GGAGCGGCAC CTGGCTGAGC GGCAACTGAC CCACCTGGAG 
GAGATGCAGC ACACCATCTC TAACGATGAG GAGAAGAGCG CCGCCGTGGT CAACGCCATC 
AGCATGTACA TGCGGCACCT TGGGGTGCGG ACCAAGAGTG GGGACAAGAA GTCCGGGAGG 
AACTTCTTTC GGAAAAAGAT GATAGGGAAC CGGCGGTCAG ATGAACCGAC CAAGACCAAG 
AAAGGCCTGA GCAGCTTCCT GGATGCCGCC CGCTGGAACC GGGGAGAGCC CCAGGATTTT 
CGACACCCGA AGGTCGAAGT TGATGCTGAG AAGCCAGGCC TTACAGAACG TAAGGGAGGT 
CAGGGGGCGC CTTCCCGGGA CCGGAATCTC GGAGGCCCTG GGCAGGACAC CCCCGGAGTT 
TCTCCGCACC TTCTGTCTGG GGACAGCCCT GACCGGGAGC CAGGTGTTGA CGCCCTCCTG 
GAGGTGGGGG ACCCGCCCCC ACAGGGCCCA GCCAGCCTGG ACGCCCCAGC GCCGCTGGAG 
AGCACGGAGG AGGCTGCCGA CACAGAAAGG CTGTCAGGGC GTCTGGGGCG CTCAGAGAGC 
CTGCGGGTGA GTGACCGCCG CCGGCCTTCC CGGGGTAGCC TCGGGGCTAA GGGCCGGGGT 
GGGGGCCGCT CCCGGAGCGA CGTGGACATG GACCCCAGCT CCGCCACGGC CGTGCTTGGC 
CCTACCCGAC GAGCCACCCC GGAGCCTGGA GATGAGGGAG AGCCGGGCCG CTCAGGACTG 
GAGCTGGAAC CAGAGGAGCC GCCTGGCTGG CGAGAGCTTG TCCCCCAGGA CATTCTGCAC 
AGCCTGCCCA AGAGCCAGGT GAAGCGGCAG GAGGTCATCA GCGAGCTCCT GGTGACGGAG 
GCTGCCCATG TGCGCATGCT CCGCGTGCTC CACGACGTCT TCTACCAGCC CATAGCGGAC 
GGGGGCTTCT ACTCCCAGGA GGAGCTCCAG AACATCTTCC CCAGCCTGGA CGAGCTCATC 
GAGGTGCATT CCCTATTCCT TGATTCCCTG ATGAAGCGGA GGCAGGACAG TGGCTACCTC 
ATCAAGGAGA TCGGAGATGT GCTGCTGGCC CGGTTCGATG GTGCCGAGGG CTCGTGGTTC 
CAGAAAATCT CCTCCCGCTT CTGTAGCCGC CAGTCGTTCG CCTTAGAGCA GCTCAAAGCC 
AAACAGCGCA AGGAGCCCCG GTTCTGTACC TTCGTGCAGG AGGCCGAGAG CCGGCCCCGG 
TGCCGCCGGC TGCAGCTGAA GGACATGATC CCCACCGAGA TGCAGCGTCT GACCAAGTAC 
CCACTGCTTC TGCAGAGCAT CGAGCAGAAC ACAGAAGAGC CGGTGGAACG GGAGAAAGTG 
GAGCGGGCAG CCGAGTGCTG CCGGGAAATT CTGCAACACG TCAACCTTGC CGTGCGGGAC 
ATGGAGGACC TGCTGCGGCT CAAAGATTAT CAGAGGCGTC TGGATTTGAC CCACCTGCGG 
CAGAGCAACG ACCCCATGCT GAGCGAGTTC AAGAACCTAG ACATCACCAA GAAGAGGTTG 
ATCTACGAGG GCCCACTGAC GTGGCGGGTG ACGAAGGACA AGGCTGTGGA GGTCCACGTG 
CTGCTGCTGG ATGACCTGCT GCTGCTGCTT CAGCGCCAGG ACGAGCGGTT CTTGCTTAAG 
TCGCACAGCC GGACGCTGAC GCCCACGCCC GATGGCAAGA CCATGCTGCG GCCAGTGCTG 
CGGCTCACCT CCGCCATGAC CCGCGAGGTG GCCACCGATC ACAAAGCCTT CTATGTCATT 
TTTACCTGGG ACCAGGAGGC CCAGATATAC GAGCTGGTGG CGCAGACGGT GTCTGAGCGG 
AGGATCTGGT GTACCCTGAT CACCGAGACC GCTGGATGCC TGAAGGTCCC CTCCCGCACC 
TCCCGACCCA AACACCGGCC CGGCCCCAGC AGCACTCGTG AACCCCTGCT TAGCAGCTCC 
GAAAACGGCA ATGGCAGCCG AGAGATGCCC CCGGCAGATG GCCGGATGGA GAGACTCTTC 
AACTCCCTCC TGCCCTTCTG CAGACAAGGC CTCGAGGGCC AGCTCGCCGC CAAGGCCCTT 
CAGAAAGTGC TGACCCTGAA ACAGCTCCTG TTGCCTTCGG AGGAAGACAG CGGGGCAGGG 
CCTCCCCTCG AAGGGGATGG GGTCCCGGGG GGCAGCCCCT TGAGCCCAAC GCAGCCCCAG 
GAAATTCGGG AGGAGCTGCT CAGCCTGGAG GAGACCATTA AACAGCTAGA GGAGGTGGAG 
GAGGAATTTT GCCGCCTGAG ACTCCTCCTG TCTCAGCTTG GGGAGAACAC CGTCCCCCAG 
TCTGGCTGTA CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF1 ( XM_024979115.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024834883.1 Bos taurus Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa48601
Clone ID Related Accession (Same CDS sequence)	XM_024979115.1 , XM_024979116.1
Accession Version	XM_024979115.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2943bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rho guanine nucleotide exchange factor 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATTCT CCAGCCCGGC AGTGCCCATG GAGGCAGAAG ACGTCGCCCG AGGGGCGGCC 
CCGGGGCCCC CCCGACCCGG CCTGGCGCCC GTCAGCATCA TTGGGGCAGA GGACGAGGAT 
TTTGAGATTG AGTTGAAGAC GAACCCTGAG GAACAAAACA ACCAGTTCCA GAACCTGGAT 
CAGGTGAAGC GGCGCCCGGC CCACCTCATG GCCCTCCTGC AGCACGTGGC CATGCAGTTT 
GAGCCGGGAC CCCTGCTCTG CTGCCTGCAT GCGGACATGC TGGGCTCCCT GGGCCCCAAG 
GAGTTCAAGA AAGCCTTCAT CGACTTCTAC CACAGCTTCC TGGATAAGCA CTCGATTCTA 
CGAGTGATGG TCCCTCCTGC CGTGGCCTTT GAACTTGACC GCACGCGGCC TGACCTCTTT 
CCTGAGGATC TCCAGCGGCA GTTCGTGCAG GAGGTGGTGC AGAGCCAGCA GGTAGCCGTG 
GTCCGGCAGC TGGAGGACTT CCGCTCCAAG AAGCTCATGG GCATGACACC CTGGGAGCAG 
GAGCTGGTCC AGCTGGGGGC CTGGGTGGCG CGGGACCGTG CCAGCTACGA GGCCCGGGAG 
CGGCACCTGG CTGAGCGGCA ACTGACCCAC CTGGAGGAGA TGCAGCACAC CATCTCTAAC 
GATGAGGAGA AGAGCGCCGC CGTGGTCAAC GCCATCAGCA TGTACATGCG GCACCTTGGG 
GTGCGGACCA AGAGTGGGGA CAAGAAGTCC GGGAGGAACT TCTTTCGGAA AAAGATGATA 
GGGAACCGGC GGTCAGATGA ACCGACCAAG ACCAAGAAAG GCCTGAGCAG CTTCCTGGAT 
GCCGCCCGCT GGAACCGGGG AGAGCCCCAG GATTTTCGAC ACCCGAAGGT CGAAGTTGAT 
GCTGAGAAGC CAGGCCTTAC AGAACGTAAG GGAGGTCAGG GGGCGCCTTC CCGGGACCGG 
AATCTCGGAG GCCCTGGGCA GGACACCCCC GGAGTTTCTC CGCACCTTCT GTCTGGGGAC 
AGCCCTGACC GGGAGCCAGG TGTTGACGCC CTCCTGGAGG TGGGGGACCC GCCCCCACAG 
GGCCCAGCCA GCCTGGACGC CCCAGCGCCG CTGGAGAGCA CGGAGGAGGC TGCCGACACA 
GAAAGAAAGT GGAAGAGGCT GTCAGGGCGT CTGGGGCGCT CAGAGAGCCT GCGGGTGAGT 
GACCGCCGCC GGCCTTCCCG GGGTAGCCTC GGGGCTAAGG GCCGGGGTGG GGGCCGCTCC 
CGGAGCGACG TGGACATGGA CCCCAGCTCC GCCACGGCCG TGCTTGGCCC TACCCGACGA 
GCCACCCCGG AGCCTGGAGA TGAGGGAGAG CCGGGCCGCT CAGGACTGGA GCTGGAACCA 
GAGGAGCCGC CTGGCTGGCG AGAGCTTGTC CCCCAGGACA TTCTGCACAG CCTGCCCAAG 
AGCCAGGTGA AGCGGCAGGA GGTCATCAGC GAGCTCCTGG TGACGGAGGC TGCCCATGTG 
CGCATGCTCC GCGTGCTCCA CGACGTCTTC TACCAGCCCA TAGCGGACGG GGGCTTCTAC 
TCCCAGGAGG AGCTCCAGAA CATCTTCCCC AGCCTGGACG AGCTCATCGA GGTGCATTCC 
CTATTCCTTG ATTCCCTGAT GAAGCGGAGG CAGGACAGTG GCTACCTCAT CAAGGAGATC 
GGAGATGTGC TGCTGGCCCG GTTCGATGGT GCCGAGGGCT CGTGGTTCCA GAAAATCTCC 
TCCCGCTTCT GTAGCCGCCA GTCGTTCGCC TTAGAGCAGC TCAAAGCCAA ACAGCGCAAG 
GAGCCCCGGT TCTGTACCTT CGTGCAGGAG GCCGAGAGCC GGCCCCGGTG CCGCCGGCTG 
CAGCTGAAGG ACATGATCCC CACCGAGATG CAGCGTCTGA CCAAGTACCC ACTGCTTCTG 
CAGAGCATCG AGCAGAACAC AGAAGAGCCG GTGGAACGGG AGAAAGTGGA GCGGGCAGCC 
GAGTGCTGCC GGGAAATTCT GCAACACGTC AACCTTGCCG TGCGGGACAT GGAGGACCTG 
CTGCGGCTCA AAGATTATCA GAGGCGTCTG GATTTGACCC ACCTGCGGCA GAGCAACGAC 
CCCATGCTGA GCGAGTTCAA GAACCTAGAC ATCACCAAGA AGAGGTTGAT CTACGAGGGC 
CCACTGACGT GGCGGGTGAC GAAGGACAAG GCTGTGGAGG TCCACGTGCT GCTGCTGGAT 
GACCTGCTGC TGCTGCTTCA GCGCCAGGAC GAGCGGTTCT TGCTTAAGTC GCACAGCCGG 
ACGCTGACGC CCACGCCCGA TGGCAAGACC ATGCTGCGGC CAGTGCTGCG GCTCACCTCC 
GCCATGACCC GCGAGGTGGC CACCGATCAC AAAGCCTTCT ATGTCATTTT TACCTGGGAC 
CAGGAGGCCC AGATATACGA GCTGGTGGCG CAGACGGTGT CTGAGCGGAG GATCTGGTGT 
ACCCTGATCA CCGAGACCGC TGGATGCCTG AAGGTCCCCT CCCGCACCTC CCGACCCAAA 
CACCGGCCCG GCCCCAGCAG CACTCGTGAA CCCCTGCTTA GCAGCTCCGA AAACGGCAAT 
GGCAGCCGAG AGATGCCCCC GGCAGATGGC CGGATGGAGA GACTCTTCAA CTCCCTCCTG 
CCCTTCTGCA GACAAGGCCT CGAGGGCCAG CTCGCCGCCA AGGCCCTTCA GAAAGTGCTG 
ACCCTGAAAC AGCTCCTGTT GCCTTCGGAG GAAGACAGCG GGGCAGGGCC TCCCCTCGAA 
GGGGATGGGG TCCCGGGGGG CAGCCCCTTG AGCCCAACGC AGCCCCAGGA AATTCGGGAG 
GAGCTGCTCA GCCTGGAGGA GACCATTAAA CAGCTAGAGG AGGTGGAGGA GGAATTTTGC 
CGCCTGAGAC TCCTCCTGTC TCAGCTTGGG GAGAACACCG TCCCCCAGTC TGGCTGTACC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024834883.1
CDS	281..3223
Translation	MEFSSPAVPMEAEDVARGAAPGPPRPGLAPVSIIGAEDEDFEIELKTNPEEQNNQFQNLDQVKRRPAHLMALLQHVAMQFEPGPLLCCLHADMLGSLGPKEFKKAFIDFYHSFLDKHSILRVMVPPAVAFELDRTRPDLFPEDLQRQFVQEVVQSQQVAVVRQLEDFRSKKLMGMTPWEQELVQLGAWVARDRASYEARERHLAERQLTHLEEMQHTISNDEEKSAAVVNAISMYMRHLGVRTKSGDKKSGRNFFRKKMIGNRRSDEPTKTKKGLSSFLDAARWNRGEPQDFRHPKVEVDAEKPGLTERKGGQGAPSRDRNLGGPGQDTPGVSPHLLSGDSPDREPGVDALLEVGDPPPQGPASLDAPAPLESTEEAADTERKWKRLSGRLGRSESLRVSDRRRPSRGSLGAKGRGGGRSRSDVDMDPSSATAVLGPTRRATPEPGDEGEPGRSGLELEPEEPPGWRELVPQDILHSLPKSQVKRQEVISELLVTEAAHVRMLRVLHDVFYQPIADGGFYSQEELQNIFPSLDELIEVHSLFLDSLMKRRQDSGYLIKEIGDVLLARFDGAEGSWFQKISSRFCSRQSFALEQLKAKQRKEPRFCTFVQEAESRPRCRRLQLKDMIPTEMQRLTKYPLLLQSIEQNTEEPVEREKVERAAECCREILQHVNLAVRDMEDLLRLKDYQRRLDLTHLRQSNDPMLSEFKNLDITKKRLIYEGPLTWRVTKDKAVEVHVLLLDDLLLLLQRQDERFLLKSHSRTLTPTPDGKTMLRPVLRLTSAMTREVATDHKAFYVIFTWDQEAQIYELVAQTVSERRIWCTLITETAGCLKVPSRTSRPKHRPGPSSTREPLLSSSENGNGSREMPPADGRMERLFNSLLPFCRQGLEGQLAAKALQKVLTLKQLLLPSEEDSGAGPPLEGDGVPGGSPLSPTQPQEIREELLSLEETIKQLEEVEEEFCRLRLLLSQLGENTVPQSGCT

Target ORF information:

RefSeq Version	XM_024979115.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024979115.1

ORF Insert Sequence:

ATGGAATTCT CCAGCCCGGC AGTGCCCATG GAGGCAGAAG ACGTCGCCCG AGGGGCGGCC 
CCGGGGCCCC CCCGACCCGG CCTGGCGCCC GTCAGCATCA TTGGGGCAGA GGACGAGGAT 
TTTGAGATTG AGTTGAAGAC GAACCCTGAG GAACAAAACA ACCAGTTCCA GAACCTGGAT 
CAGGTGAAGC GGCGCCCGGC CCACCTCATG GCCCTCCTGC AGCACGTGGC CATGCAGTTT 
GAGCCGGGAC CCCTGCTCTG CTGCCTGCAT GCGGACATGC TGGGCTCCCT GGGCCCCAAG 
GAGTTCAAGA AAGCCTTCAT CGACTTCTAC CACAGCTTCC TGGATAAGCA CTCGATTCTA 
CGAGTGATGG TCCCTCCTGC CGTGGCCTTT GAACTTGACC GCACGCGGCC TGACCTCTTT 
CCTGAGGATC TCCAGCGGCA GTTCGTGCAG GAGGTGGTGC AGAGCCAGCA GGTAGCCGTG 
GTCCGGCAGC TGGAGGACTT CCGCTCCAAG AAGCTCATGG GCATGACACC CTGGGAGCAG 
GAGCTGGTCC AGCTGGGGGC CTGGGTGGCG CGGGACCGTG CCAGCTACGA GGCCCGGGAG 
CGGCACCTGG CTGAGCGGCA ACTGACCCAC CTGGAGGAGA TGCAGCACAC CATCTCTAAC 
GATGAGGAGA AGAGCGCCGC CGTGGTCAAC GCCATCAGCA TGTACATGCG GCACCTTGGG 
GTGCGGACCA AGAGTGGGGA CAAGAAGTCC GGGAGGAACT TCTTTCGGAA AAAGATGATA 
GGGAACCGGC GGTCAGATGA ACCGACCAAG ACCAAGAAAG GCCTGAGCAG CTTCCTGGAT 
GCCGCCCGCT GGAACCGGGG AGAGCCCCAG GATTTTCGAC ACCCGAAGGT CGAAGTTGAT 
GCTGAGAAGC CAGGCCTTAC AGAACGTAAG GGAGGTCAGG GGGCGCCTTC CCGGGACCGG 
AATCTCGGAG GCCCTGGGCA GGACACCCCC GGAGTTTCTC CGCACCTTCT GTCTGGGGAC 
AGCCCTGACC GGGAGCCAGG TGTTGACGCC CTCCTGGAGG TGGGGGACCC GCCCCCACAG 
GGCCCAGCCA GCCTGGACGC CCCAGCGCCG CTGGAGAGCA CGGAGGAGGC TGCCGACACA 
GAAAGAAAGT GGAAGAGGCT GTCAGGGCGT CTGGGGCGCT CAGAGAGCCT GCGGGTGAGT 
GACCGCCGCC GGCCTTCCCG GGGTAGCCTC GGGGCTAAGG GCCGGGGTGG GGGCCGCTCC 
CGGAGCGACG TGGACATGGA CCCCAGCTCC GCCACGGCCG TGCTTGGCCC TACCCGACGA 
GCCACCCCGG AGCCTGGAGA TGAGGGAGAG CCGGGCCGCT CAGGACTGGA GCTGGAACCA 
GAGGAGCCGC CTGGCTGGCG AGAGCTTGTC CCCCAGGACA TTCTGCACAG CCTGCCCAAG 
AGCCAGGTGA AGCGGCAGGA GGTCATCAGC GAGCTCCTGG TGACGGAGGC TGCCCATGTG 
CGCATGCTCC GCGTGCTCCA CGACGTCTTC TACCAGCCCA TAGCGGACGG GGGCTTCTAC 
TCCCAGGAGG AGCTCCAGAA CATCTTCCCC AGCCTGGACG AGCTCATCGA GGTGCATTCC 
CTATTCCTTG ATTCCCTGAT GAAGCGGAGG CAGGACAGTG GCTACCTCAT CAAGGAGATC 
GGAGATGTGC TGCTGGCCCG GTTCGATGGT GCCGAGGGCT CGTGGTTCCA GAAAATCTCC 
TCCCGCTTCT GTAGCCGCCA GTCGTTCGCC TTAGAGCAGC TCAAAGCCAA ACAGCGCAAG 
GAGCCCCGGT TCTGTACCTT CGTGCAGGAG GCCGAGAGCC GGCCCCGGTG CCGCCGGCTG 
CAGCTGAAGG ACATGATCCC CACCGAGATG CAGCGTCTGA CCAAGTACCC ACTGCTTCTG 
CAGAGCATCG AGCAGAACAC AGAAGAGCCG GTGGAACGGG AGAAAGTGGA GCGGGCAGCC 
GAGTGCTGCC GGGAAATTCT GCAACACGTC AACCTTGCCG TGCGGGACAT GGAGGACCTG 
CTGCGGCTCA AAGATTATCA GAGGCGTCTG GATTTGACCC ACCTGCGGCA GAGCAACGAC 
CCCATGCTGA GCGAGTTCAA GAACCTAGAC ATCACCAAGA AGAGGTTGAT CTACGAGGGC 
CCACTGACGT GGCGGGTGAC GAAGGACAAG GCTGTGGAGG TCCACGTGCT GCTGCTGGAT 
GACCTGCTGC TGCTGCTTCA GCGCCAGGAC GAGCGGTTCT TGCTTAAGTC GCACAGCCGG 
ACGCTGACGC CCACGCCCGA TGGCAAGACC ATGCTGCGGC CAGTGCTGCG GCTCACCTCC 
GCCATGACCC GCGAGGTGGC CACCGATCAC AAAGCCTTCT ATGTCATTTT TACCTGGGAC 
CAGGAGGCCC AGATATACGA GCTGGTGGCG CAGACGGTGT CTGAGCGGAG GATCTGGTGT 
ACCCTGATCA CCGAGACCGC TGGATGCCTG AAGGTCCCCT CCCGCACCTC CCGACCCAAA 
CACCGGCCCG GCCCCAGCAG CACTCGTGAA CCCCTGCTTA GCAGCTCCGA AAACGGCAAT 
GGCAGCCGAG AGATGCCCCC GGCAGATGGC CGGATGGAGA GACTCTTCAA CTCCCTCCTG 
CCCTTCTGCA GACAAGGCCT CGAGGGCCAG CTCGCCGCCA AGGCCCTTCA GAAAGTGCTG 
ACCCTGAAAC AGCTCCTGTT GCCTTCGGAG GAAGACAGCG GGGCAGGGCC TCCCCTCGAA 
GGGGATGGGG TCCCGGGGGG CAGCCCCTTG AGCCCAACGC AGCCCCAGGA AATTCGGGAG 
GAGCTGCTCA GCCTGGAGGA GACCATTAAA CAGCTAGAGG AGGTGGAGGA GGAATTTTGC 
CGCCTGAGAC TCCTCCTGTC TCAGCTTGGG GAGAACACCG TCCCCCAGTC TGGCTGTACC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF1 ( XM_024979116.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024834884.1 Bos taurus Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBa48601
Clone ID Related Accession (Same CDS sequence)	XM_024979115.1 , XM_024979116.1
Accession Version	XM_024979116.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2943bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	rho guanine nucleotide exchange factor 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATTCT CCAGCCCGGC AGTGCCCATG GAGGCAGAAG ACGTCGCCCG AGGGGCGGCC 
CCGGGGCCCC CCCGACCCGG CCTGGCGCCC GTCAGCATCA TTGGGGCAGA GGACGAGGAT 
TTTGAGATTG AGTTGAAGAC GAACCCTGAG GAACAAAACA ACCAGTTCCA GAACCTGGAT 
CAGGTGAAGC GGCGCCCGGC CCACCTCATG GCCCTCCTGC AGCACGTGGC CATGCAGTTT 
GAGCCGGGAC CCCTGCTCTG CTGCCTGCAT GCGGACATGC TGGGCTCCCT GGGCCCCAAG 
GAGTTCAAGA AAGCCTTCAT CGACTTCTAC CACAGCTTCC TGGATAAGCA CTCGATTCTA 
CGAGTGATGG TCCCTCCTGC CGTGGCCTTT GAACTTGACC GCACGCGGCC TGACCTCTTT 
CCTGAGGATC TCCAGCGGCA GTTCGTGCAG GAGGTGGTGC AGAGCCAGCA GGTAGCCGTG 
GTCCGGCAGC TGGAGGACTT CCGCTCCAAG AAGCTCATGG GCATGACACC CTGGGAGCAG 
GAGCTGGTCC AGCTGGGGGC CTGGGTGGCG CGGGACCGTG CCAGCTACGA GGCCCGGGAG 
CGGCACCTGG CTGAGCGGCA ACTGACCCAC CTGGAGGAGA TGCAGCACAC CATCTCTAAC 
GATGAGGAGA AGAGCGCCGC CGTGGTCAAC GCCATCAGCA TGTACATGCG GCACCTTGGG 
GTGCGGACCA AGAGTGGGGA CAAGAAGTCC GGGAGGAACT TCTTTCGGAA AAAGATGATA 
GGGAACCGGC GGTCAGATGA ACCGACCAAG ACCAAGAAAG GCCTGAGCAG CTTCCTGGAT 
GCCGCCCGCT GGAACCGGGG AGAGCCCCAG GATTTTCGAC ACCCGAAGGT CGAAGTTGAT 
GCTGAGAAGC CAGGCCTTAC AGAACGTAAG GGAGGTCAGG GGGCGCCTTC CCGGGACCGG 
AATCTCGGAG GCCCTGGGCA GGACACCCCC GGAGTTTCTC CGCACCTTCT GTCTGGGGAC 
AGCCCTGACC GGGAGCCAGG TGTTGACGCC CTCCTGGAGG TGGGGGACCC GCCCCCACAG 
GGCCCAGCCA GCCTGGACGC CCCAGCGCCG CTGGAGAGCA CGGAGGAGGC TGCCGACACA 
GAAAGAAAGT GGAAGAGGCT GTCAGGGCGT CTGGGGCGCT CAGAGAGCCT GCGGGTGAGT 
GACCGCCGCC GGCCTTCCCG GGGTAGCCTC GGGGCTAAGG GCCGGGGTGG GGGCCGCTCC 
CGGAGCGACG TGGACATGGA CCCCAGCTCC GCCACGGCCG TGCTTGGCCC TACCCGACGA 
GCCACCCCGG AGCCTGGAGA TGAGGGAGAG CCGGGCCGCT CAGGACTGGA GCTGGAACCA 
GAGGAGCCGC CTGGCTGGCG AGAGCTTGTC CCCCAGGACA TTCTGCACAG CCTGCCCAAG 
AGCCAGGTGA AGCGGCAGGA GGTCATCAGC GAGCTCCTGG TGACGGAGGC TGCCCATGTG 
CGCATGCTCC GCGTGCTCCA CGACGTCTTC TACCAGCCCA TAGCGGACGG GGGCTTCTAC 
TCCCAGGAGG AGCTCCAGAA CATCTTCCCC AGCCTGGACG AGCTCATCGA GGTGCATTCC 
CTATTCCTTG ATTCCCTGAT GAAGCGGAGG CAGGACAGTG GCTACCTCAT CAAGGAGATC 
GGAGATGTGC TGCTGGCCCG GTTCGATGGT GCCGAGGGCT CGTGGTTCCA GAAAATCTCC 
TCCCGCTTCT GTAGCCGCCA GTCGTTCGCC TTAGAGCAGC TCAAAGCCAA ACAGCGCAAG 
GAGCCCCGGT TCTGTACCTT CGTGCAGGAG GCCGAGAGCC GGCCCCGGTG CCGCCGGCTG 
CAGCTGAAGG ACATGATCCC CACCGAGATG CAGCGTCTGA CCAAGTACCC ACTGCTTCTG 
CAGAGCATCG AGCAGAACAC AGAAGAGCCG GTGGAACGGG AGAAAGTGGA GCGGGCAGCC 
GAGTGCTGCC GGGAAATTCT GCAACACGTC AACCTTGCCG TGCGGGACAT GGAGGACCTG 
CTGCGGCTCA AAGATTATCA GAGGCGTCTG GATTTGACCC ACCTGCGGCA GAGCAACGAC 
CCCATGCTGA GCGAGTTCAA GAACCTAGAC ATCACCAAGA AGAGGTTGAT CTACGAGGGC 
CCACTGACGT GGCGGGTGAC GAAGGACAAG GCTGTGGAGG TCCACGTGCT GCTGCTGGAT 
GACCTGCTGC TGCTGCTTCA GCGCCAGGAC GAGCGGTTCT TGCTTAAGTC GCACAGCCGG 
ACGCTGACGC CCACGCCCGA TGGCAAGACC ATGCTGCGGC CAGTGCTGCG GCTCACCTCC 
GCCATGACCC GCGAGGTGGC CACCGATCAC AAAGCCTTCT ATGTCATTTT TACCTGGGAC 
CAGGAGGCCC AGATATACGA GCTGGTGGCG CAGACGGTGT CTGAGCGGAG GATCTGGTGT 
ACCCTGATCA CCGAGACCGC TGGATGCCTG AAGGTCCCCT CCCGCACCTC CCGACCCAAA 
CACCGGCCCG GCCCCAGCAG CACTCGTGAA CCCCTGCTTA GCAGCTCCGA AAACGGCAAT 
GGCAGCCGAG AGATGCCCCC GGCAGATGGC CGGATGGAGA GACTCTTCAA CTCCCTCCTG 
CCCTTCTGCA GACAAGGCCT CGAGGGCCAG CTCGCCGCCA AGGCCCTTCA GAAAGTGCTG 
ACCCTGAAAC AGCTCCTGTT GCCTTCGGAG GAAGACAGCG GGGCAGGGCC TCCCCTCGAA 
GGGGATGGGG TCCCGGGGGG CAGCCCCTTG AGCCCAACGC AGCCCCAGGA AATTCGGGAG 
GAGCTGCTCA GCCTGGAGGA GACCATTAAA CAGCTAGAGG AGGTGGAGGA GGAATTTTGC 
CGCCTGAGAC TCCTCCTGTC TCAGCTTGGG GAGAACACCG TCCCCCAGTC TGGCTGTACC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024834884.1
CDS	404..3346
Translation	MEFSSPAVPMEAEDVARGAAPGPPRPGLAPVSIIGAEDEDFEIELKTNPEEQNNQFQNLDQVKRRPAHLMALLQHVAMQFEPGPLLCCLHADMLGSLGPKEFKKAFIDFYHSFLDKHSILRVMVPPAVAFELDRTRPDLFPEDLQRQFVQEVVQSQQVAVVRQLEDFRSKKLMGMTPWEQELVQLGAWVARDRASYEARERHLAERQLTHLEEMQHTISNDEEKSAAVVNAISMYMRHLGVRTKSGDKKSGRNFFRKKMIGNRRSDEPTKTKKGLSSFLDAARWNRGEPQDFRHPKVEVDAEKPGLTERKGGQGAPSRDRNLGGPGQDTPGVSPHLLSGDSPDREPGVDALLEVGDPPPQGPASLDAPAPLESTEEAADTERKWKRLSGRLGRSESLRVSDRRRPSRGSLGAKGRGGGRSRSDVDMDPSSATAVLGPTRRATPEPGDEGEPGRSGLELEPEEPPGWRELVPQDILHSLPKSQVKRQEVISELLVTEAAHVRMLRVLHDVFYQPIADGGFYSQEELQNIFPSLDELIEVHSLFLDSLMKRRQDSGYLIKEIGDVLLARFDGAEGSWFQKISSRFCSRQSFALEQLKAKQRKEPRFCTFVQEAESRPRCRRLQLKDMIPTEMQRLTKYPLLLQSIEQNTEEPVEREKVERAAECCREILQHVNLAVRDMEDLLRLKDYQRRLDLTHLRQSNDPMLSEFKNLDITKKRLIYEGPLTWRVTKDKAVEVHVLLLDDLLLLLQRQDERFLLKSHSRTLTPTPDGKTMLRPVLRLTSAMTREVATDHKAFYVIFTWDQEAQIYELVAQTVSERRIWCTLITETAGCLKVPSRTSRPKHRPGPSSTREPLLSSSENGNGSREMPPADGRMERLFNSLLPFCRQGLEGQLAAKALQKVLTLKQLLLPSEEDSGAGPPLEGDGVPGGSPLSPTQPQEIREELLSLEETIKQLEEVEEEFCRLRLLLSQLGENTVPQSGCT

Target ORF information:

RefSeq Version	XM_024979116.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024979116.1

ORF Insert Sequence:

ATGGAATTCT CCAGCCCGGC AGTGCCCATG GAGGCAGAAG ACGTCGCCCG AGGGGCGGCC 
CCGGGGCCCC CCCGACCCGG CCTGGCGCCC GTCAGCATCA TTGGGGCAGA GGACGAGGAT 
TTTGAGATTG AGTTGAAGAC GAACCCTGAG GAACAAAACA ACCAGTTCCA GAACCTGGAT 
CAGGTGAAGC GGCGCCCGGC CCACCTCATG GCCCTCCTGC AGCACGTGGC CATGCAGTTT 
GAGCCGGGAC CCCTGCTCTG CTGCCTGCAT GCGGACATGC TGGGCTCCCT GGGCCCCAAG 
GAGTTCAAGA AAGCCTTCAT CGACTTCTAC CACAGCTTCC TGGATAAGCA CTCGATTCTA 
CGAGTGATGG TCCCTCCTGC CGTGGCCTTT GAACTTGACC GCACGCGGCC TGACCTCTTT 
CCTGAGGATC TCCAGCGGCA GTTCGTGCAG GAGGTGGTGC AGAGCCAGCA GGTAGCCGTG 
GTCCGGCAGC TGGAGGACTT CCGCTCCAAG AAGCTCATGG GCATGACACC CTGGGAGCAG 
GAGCTGGTCC AGCTGGGGGC CTGGGTGGCG CGGGACCGTG CCAGCTACGA GGCCCGGGAG 
CGGCACCTGG CTGAGCGGCA ACTGACCCAC CTGGAGGAGA TGCAGCACAC CATCTCTAAC 
GATGAGGAGA AGAGCGCCGC CGTGGTCAAC GCCATCAGCA TGTACATGCG GCACCTTGGG 
GTGCGGACCA AGAGTGGGGA CAAGAAGTCC GGGAGGAACT TCTTTCGGAA AAAGATGATA 
GGGAACCGGC GGTCAGATGA ACCGACCAAG ACCAAGAAAG GCCTGAGCAG CTTCCTGGAT 
GCCGCCCGCT GGAACCGGGG AGAGCCCCAG GATTTTCGAC ACCCGAAGGT CGAAGTTGAT 
GCTGAGAAGC CAGGCCTTAC AGAACGTAAG GGAGGTCAGG GGGCGCCTTC CCGGGACCGG 
AATCTCGGAG GCCCTGGGCA GGACACCCCC GGAGTTTCTC CGCACCTTCT GTCTGGGGAC 
AGCCCTGACC GGGAGCCAGG TGTTGACGCC CTCCTGGAGG TGGGGGACCC GCCCCCACAG 
GGCCCAGCCA GCCTGGACGC CCCAGCGCCG CTGGAGAGCA CGGAGGAGGC TGCCGACACA 
GAAAGAAAGT GGAAGAGGCT GTCAGGGCGT CTGGGGCGCT CAGAGAGCCT GCGGGTGAGT 
GACCGCCGCC GGCCTTCCCG GGGTAGCCTC GGGGCTAAGG GCCGGGGTGG GGGCCGCTCC 
CGGAGCGACG TGGACATGGA CCCCAGCTCC GCCACGGCCG TGCTTGGCCC TACCCGACGA 
GCCACCCCGG AGCCTGGAGA TGAGGGAGAG CCGGGCCGCT CAGGACTGGA GCTGGAACCA 
GAGGAGCCGC CTGGCTGGCG AGAGCTTGTC CCCCAGGACA TTCTGCACAG CCTGCCCAAG 
AGCCAGGTGA AGCGGCAGGA GGTCATCAGC GAGCTCCTGG TGACGGAGGC TGCCCATGTG 
CGCATGCTCC GCGTGCTCCA CGACGTCTTC TACCAGCCCA TAGCGGACGG GGGCTTCTAC 
TCCCAGGAGG AGCTCCAGAA CATCTTCCCC AGCCTGGACG AGCTCATCGA GGTGCATTCC 
CTATTCCTTG ATTCCCTGAT GAAGCGGAGG CAGGACAGTG GCTACCTCAT CAAGGAGATC 
GGAGATGTGC TGCTGGCCCG GTTCGATGGT GCCGAGGGCT CGTGGTTCCA GAAAATCTCC 
TCCCGCTTCT GTAGCCGCCA GTCGTTCGCC TTAGAGCAGC TCAAAGCCAA ACAGCGCAAG 
GAGCCCCGGT TCTGTACCTT CGTGCAGGAG GCCGAGAGCC GGCCCCGGTG CCGCCGGCTG 
CAGCTGAAGG ACATGATCCC CACCGAGATG CAGCGTCTGA CCAAGTACCC ACTGCTTCTG 
CAGAGCATCG AGCAGAACAC AGAAGAGCCG GTGGAACGGG AGAAAGTGGA GCGGGCAGCC 
GAGTGCTGCC GGGAAATTCT GCAACACGTC AACCTTGCCG TGCGGGACAT GGAGGACCTG 
CTGCGGCTCA AAGATTATCA GAGGCGTCTG GATTTGACCC ACCTGCGGCA GAGCAACGAC 
CCCATGCTGA GCGAGTTCAA GAACCTAGAC ATCACCAAGA AGAGGTTGAT CTACGAGGGC 
CCACTGACGT GGCGGGTGAC GAAGGACAAG GCTGTGGAGG TCCACGTGCT GCTGCTGGAT 
GACCTGCTGC TGCTGCTTCA GCGCCAGGAC GAGCGGTTCT TGCTTAAGTC GCACAGCCGG 
ACGCTGACGC CCACGCCCGA TGGCAAGACC ATGCTGCGGC CAGTGCTGCG GCTCACCTCC 
GCCATGACCC GCGAGGTGGC CACCGATCAC AAAGCCTTCT ATGTCATTTT TACCTGGGAC 
CAGGAGGCCC AGATATACGA GCTGGTGGCG CAGACGGTGT CTGAGCGGAG GATCTGGTGT 
ACCCTGATCA CCGAGACCGC TGGATGCCTG AAGGTCCCCT CCCGCACCTC CCGACCCAAA 
CACCGGCCCG GCCCCAGCAG CACTCGTGAA CCCCTGCTTA GCAGCTCCGA AAACGGCAAT 
GGCAGCCGAG AGATGCCCCC GGCAGATGGC CGGATGGAGA GACTCTTCAA CTCCCTCCTG 
CCCTTCTGCA GACAAGGCCT CGAGGGCCAG CTCGCCGCCA AGGCCCTTCA GAAAGTGCTG 
ACCCTGAAAC AGCTCCTGTT GCCTTCGGAG GAAGACAGCG GGGCAGGGCC TCCCCTCGAA 
GGGGATGGGG TCCCGGGGGG CAGCCCCTTG AGCCCAACGC AGCCCCAGGA AATTCGGGAG 
GAGCTGCTCA GCCTGGAGGA GACCATTAAA CAGCTAGAGG AGGTGGAGGA GGAATTTTGC 
CGCCTGAGAC TCCTCCTGTC TCAGCTTGGG GAGAACACCG TCCCCCAGTC TGGCTGTACC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF1 ( XM_010815123.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010813425.1 Bos taurus Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa48603
Clone ID Related Accession (Same CDS sequence)	XM_010815123.2 , XM_010823709.2
Accession Version	XM_010815123.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2787bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	rho guanine nucleotide exchange factor 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104466.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010815123.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGAGA GAGGCGAGAC AGCGAGCGAC ACGGACAGCC CGGCAGTGCC CATGGAGGCA 
GAAGACGTCG CCCGAGGGGC GGCCCCGGGG CCCCCCCGAC CCGGCCTGGC GCCCGTCAGC 
ATCATTGGGG CAGAGGACGA GGATTTTGAG ATTGAGTTGA AGACGAACCC TGAGGAACAA 
AACAACCAGT TCCAGAACCT GGATCAGGTG AAGCGGCGCC CGGCCCACCT CATGGCCCTC 
CTGCAGCACG TGGCCATGCA GTTTGAGCCG GGACCCCTGC TCTGCTGCCT GCATGCGGAC 
ATGCTGGGCT CCCTGGGCCC CAAGGAGTTC AAGAAAGCCT TCATCGACTT CTACCACAGC 
TTCCTGGATA AGCACTCGAT TCTACGAGTG ATGGTCCCTC CTGCCGTGGC CTTTGAACTT 
GACCGCACGC GGCCTGACCT CTTTCCTGAG GATCTCCAGC GGCAGTTCGT GCAGGAGGTG 
GTGCAGAGCC AGCAGGTAGC CGTGGTCCGG CAGCTGGAGG ACTTCCGCTC CAAGAAGCTC 
ATGGGCATGA CACCCTGGGA GCAGGAGCTG GTCCAGCTGG GGGCCTGGGT GGCGCGGGAC 
CGTGCCAGCT ACGAGGCCCG GGAGCGGCAC CTGGCTGAGC GGCAACTGAC CCACCTGGAG 
GAGATGCAGC ACACCATCTC TAACGATGAG GAGAAGAGCG CCGCCGTGGT CAACGCCATC 
AGCATGTACA TGCGGCACCT TGGGGTGCGG ACCAAGAGTG GGGACAAGAA GTCCGGGAGG 
AACTTCTTTC GGAAAAAGAT GATAGGGAAC CGGCGGTCAG ATGAACCGAC CAAGACCAAG 
AAAGGCCTGA GCAGCTTCCT GGATGCCGCC CGCTGGAACC GGGGAGAGCC CCAGGATTTT 
CGACACCCGA AGGTCGAAGT TGATGCTGAG AAGCCAGGCC TTACAGAACG TAAGGGAGGT 
CAGGGGGCGC CTTCCCGGGA CCGGAATCTC GGAGGCCCTG GGCAGGACAC CCCCGGAGTT 
TCTCCGCACC TTCTGTCTGG GGACAGCCCT GACCGGGAGC CAGGTGTTGA CGCCCTCCTG 
GAGGTGGGGG ACCCGCCCCC ACAGGGCCCA GCCAGCCTGG ACGCCCCAGC GCCGCTGGAG 
AGCACGGAGG AGGCTGCCGA CACAGAAAGC CCGGAGCCTG GAGATGAGGG AGAGCCGGGC 
CGCTCAGGAC TGGAGCTGGA ACCAGAGGAG CCGCCTGGCT GGCGAGAGCT TGTCCCCCAG 
GACATTCTGC ACAGCCTGCC CAAGAGCCAG GTGAAGCGGC AGGAGGTCAT CAGCGAGCTC 
CTGGTGACGG AGGCTGCCCA TGTGCGCATG CTCCGCGTGC TCCACGACGT CTTCTACCAG 
CCCATAGCGG ACGGGGGCTT CTACTCCCAG GAGGAGCTCC AGAACATCTT CCCCAGCCTG 
GACGAGCTCA TCGAGGTGCA TTCCCTATTC CTTGATTCCC TGATGAAGCG GAGGCAGGAC 
AGTGGCTACC TCATCAAGGA GATCGGAGAT GTGCTGCTGG CCCGGTTCGA TGGTGCCGAG 
GGCTCGTGGT TCCAGAAAAT CTCCTCCCGC TTCTGTAGCC GCCAGTCGTT CGCCTTAGAG 
CAGCTCAAAG CCAAACAGCG CAAGGAGCCC CGGTTCTGTA CCTTCGTGCA GGAGGCCGAG 
AGCCGGCCCC GGTGCCGCCG GCTGCAGCTG AAGGACATGA TCCCCACCGA GATGCAGCGT 
CTGACCAAGT ACCCACTGCT TCTGCAGAGC ATCGAGCAGA ACACAGAAGA GCCGGTGGAA 
CGGGAGAAAG TGGAGCGGGC AGCCGAGTGC TGCCGGGAAA TTCTGCAACA CGTCAACCTT 
GCCGTGCGGG ACATGGAGGA CCTGCTGCGG CTCAAAGATT ATCAGAGGCG TCTGGATTTG 
ACCCACCTGC GGCAGAGCAA CGACCCCATG CTGAGCGAGT TCAAGAACCT AGACATCACC 
AAGAAGAGGT TGATCTACGA GGGCCCACTG ACGTGGCGGG TGACGAAGGA CAAGGCTGTG 
GAGGTCCACG TGCTGCTGCT GGATGACCTG CTGCTGCTGC TTCAGCGCCA GGACGAGCGG 
TTCTTGCTTA AGTCGCACAG CCGGACGCTG ACGCCCACGC CCGATGGCAA GACCATGCTG 
CGGCCAGTGC TGCGGCTCAC CTCCGCCATG ACCCGCGAGG TGGCCACCGA TCACAAAGCC 
TTCTATGTCA TTTTTACCTG GGACCAGGAG GCCCAGATAT ACGAGCTGGT GGCGCAGACG 
GTGTCTGAGC GGAGGATCTG GTGTACCCTG ATCACCGAGA CCGCTGGATG CCTGAAGGTC 
CCCTCCCGCA CCTCCCGACC CAAACACCGG CCCGGCCCCA GCAGCACTCG TGAACCCCTG 
CTTAGCAGCT CCGAAAACGG CAATGGCAGC CGAGAGATGC CCCCGGCAGA TGGCCGGATG 
GAGAGACTCT TCAACTCCCT CCTGCCCTTC TGCAGACAAG GCCTCGAGGG CCAGCTCGCC 
GCCAAGGCCC TTCAGAAAGT GCTGACCCTG AAACAGCTCC TGTTGCCTTC GGAGGAAGAC 
AGCGGGGCAG GGCCTCCCCT CGAAGGGGAT GGGGTCCCGG GGGGCAGCCC CTTGAGCCCA 
ACGCAGCCCC AGGAAATTCG GGAGGAGCTG CTCAGCCTGG AGGAGACCAT TAAACAGCTA 
GAGGAGGTGG AGGAGGAATT TTGCCGCCTG AGACTCCTCC TGTCTCAGCT TGGGGAGAAC 
ACCGTCCCCC AGTCTGGCTG TACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010813425.1
CDS	138..2924
Translation	MGERGETASDTDSPAVPMEAEDVARGAAPGPPRPGLAPVSIIGAEDEDFEIELKTNPEEQNNQFQNLDQVKRRPAHLMALLQHVAMQFEPGPLLCCLHADMLGSLGPKEFKKAFIDFYHSFLDKHSILRVMVPPAVAFELDRTRPDLFPEDLQRQFVQEVVQSQQVAVVRQLEDFRSKKLMGMTPWEQELVQLGAWVARDRASYEARERHLAERQLTHLEEMQHTISNDEEKSAAVVNAISMYMRHLGVRTKSGDKKSGRNFFRKKMIGNRRSDEPTKTKKGLSSFLDAARWNRGEPQDFRHPKVEVDAEKPGLTERKGGQGAPSRDRNLGGPGQDTPGVSPHLLSGDSPDREPGVDALLEVGDPPPQGPASLDAPAPLESTEEAADTESPEPGDEGEPGRSGLELEPEEPPGWRELVPQDILHSLPKSQVKRQEVISELLVTEAAHVRMLRVLHDVFYQPIADGGFYSQEELQNIFPSLDELIEVHSLFLDSLMKRRQDSGYLIKEIGDVLLARFDGAEGSWFQKISSRFCSRQSFALEQLKAKQRKEPRFCTFVQEAESRPRCRRLQLKDMIPTEMQRLTKYPLLLQSIEQNTEEPVEREKVERAAECCREILQHVNLAVRDMEDLLRLKDYQRRLDLTHLRQSNDPMLSEFKNLDITKKRLIYEGPLTWRVTKDKAVEVHVLLLDDLLLLLQRQDERFLLKSHSRTLTPTPDGKTMLRPVLRLTSAMTREVATDHKAFYVIFTWDQEAQIYELVAQTVSERRIWCTLITETAGCLKVPSRTSRPKHRPGPSSTREPLLSSSENGNGSREMPPADGRMERLFNSLLPFCRQGLEGQLAAKALQKVLTLKQLLLPSEEDSGAGPPLEGDGVPGGSPLSPTQPQEIREELLSLEETIKQLEEVEEEFCRLRLLLSQLGENTVPQSGCT

Target ORF information:

RefSeq Version	XM_010815123.2
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010815123.2

ORF Insert Sequence:

ATGGGAGAGA GAGGCGAGAC AGCGAGCGAC ACGGACAGCC CGGCAGTGCC CATGGAGGCA 
GAAGACGTCG CCCGAGGGGC GGCCCCGGGG CCCCCCCGAC CCGGCCTGGC GCCCGTCAGC 
ATCATTGGGG CAGAGGACGA GGATTTTGAG ATTGAGTTGA AGACGAACCC TGAGGAACAA 
AACAACCAGT TCCAGAACCT GGATCAGGTG AAGCGGCGCC CGGCCCACCT CATGGCCCTC 
CTGCAGCACG TGGCCATGCA GTTTGAGCCG GGACCCCTGC TCTGCTGCCT GCATGCGGAC 
ATGCTGGGCT CCCTGGGCCC CAAGGAGTTC AAGAAAGCCT TCATCGACTT CTACCACAGC 
TTCCTGGATA AGCACTCGAT TCTACGAGTG ATGGTCCCTC CTGCCGTGGC CTTTGAACTT 
GACCGCACGC GGCCTGACCT CTTTCCTGAG GATCTCCAGC GGCAGTTCGT GCAGGAGGTG 
GTGCAGAGCC AGCAGGTAGC CGTGGTCCGG CAGCTGGAGG ACTTCCGCTC CAAGAAGCTC 
ATGGGCATGA CACCCTGGGA GCAGGAGCTG GTCCAGCTGG GGGCCTGGGT GGCGCGGGAC 
CGTGCCAGCT ACGAGGCCCG GGAGCGGCAC CTGGCTGAGC GGCAACTGAC CCACCTGGAG 
GAGATGCAGC ACACCATCTC TAACGATGAG GAGAAGAGCG CCGCCGTGGT CAACGCCATC 
AGCATGTACA TGCGGCACCT TGGGGTGCGG ACCAAGAGTG GGGACAAGAA GTCCGGGAGG 
AACTTCTTTC GGAAAAAGAT GATAGGGAAC CGGCGGTCAG ATGAACCGAC CAAGACCAAG 
AAAGGCCTGA GCAGCTTCCT GGATGCCGCC CGCTGGAACC GGGGAGAGCC CCAGGATTTT 
CGACACCCGA AGGTCGAAGT TGATGCTGAG AAGCCAGGCC TTACAGAACG TAAGGGAGGT 
CAGGGGGCGC CTTCCCGGGA CCGGAATCTC GGAGGCCCTG GGCAGGACAC CCCCGGAGTT 
TCTCCGCACC TTCTGTCTGG GGACAGCCCT GACCGGGAGC CAGGTGTTGA CGCCCTCCTG 
GAGGTGGGGG ACCCGCCCCC ACAGGGCCCA GCCAGCCTGG ACGCCCCAGC GCCGCTGGAG 
AGCACGGAGG AGGCTGCCGA CACAGAAAGC CCGGAGCCTG GAGATGAGGG AGAGCCGGGC 
CGCTCAGGAC TGGAGCTGGA ACCAGAGGAG CCGCCTGGCT GGCGAGAGCT TGTCCCCCAG 
GACATTCTGC ACAGCCTGCC CAAGAGCCAG GTGAAGCGGC AGGAGGTCAT CAGCGAGCTC 
CTGGTGACGG AGGCTGCCCA TGTGCGCATG CTCCGCGTGC TCCACGACGT CTTCTACCAG 
CCCATAGCGG ACGGGGGCTT CTACTCCCAG GAGGAGCTCC AGAACATCTT CCCCAGCCTG 
GACGAGCTCA TCGAGGTGCA TTCCCTATTC CTTGATTCCC TGATGAAGCG GAGGCAGGAC 
AGTGGCTACC TCATCAAGGA GATCGGAGAT GTGCTGCTGG CCCGGTTCGA TGGTGCCGAG 
GGCTCGTGGT TCCAGAAAAT CTCCTCCCGC TTCTGTAGCC GCCAGTCGTT CGCCTTAGAG 
CAGCTCAAAG CCAAACAGCG CAAGGAGCCC CGGTTCTGTA CCTTCGTGCA GGAGGCCGAG 
AGCCGGCCCC GGTGCCGCCG GCTGCAGCTG AAGGACATGA TCCCCACCGA GATGCAGCGT 
CTGACCAAGT ACCCACTGCT TCTGCAGAGC ATCGAGCAGA ACACAGAAGA GCCGGTGGAA 
CGGGAGAAAG TGGAGCGGGC AGCCGAGTGC TGCCGGGAAA TTCTGCAACA CGTCAACCTT 
GCCGTGCGGG ACATGGAGGA CCTGCTGCGG CTCAAAGATT ATCAGAGGCG TCTGGATTTG 
ACCCACCTGC GGCAGAGCAA CGACCCCATG CTGAGCGAGT TCAAGAACCT AGACATCACC 
AAGAAGAGGT TGATCTACGA GGGCCCACTG ACGTGGCGGG TGACGAAGGA CAAGGCTGTG 
GAGGTCCACG TGCTGCTGCT GGATGACCTG CTGCTGCTGC TTCAGCGCCA GGACGAGCGG 
TTCTTGCTTA AGTCGCACAG CCGGACGCTG ACGCCCACGC CCGATGGCAA GACCATGCTG 
CGGCCAGTGC TGCGGCTCAC CTCCGCCATG ACCCGCGAGG TGGCCACCGA TCACAAAGCC 
TTCTATGTCA TTTTTACCTG GGACCAGGAG GCCCAGATAT ACGAGCTGGT GGCGCAGACG 
GTGTCTGAGC GGAGGATCTG GTGTACCCTG ATCACCGAGA CCGCTGGATG CCTGAAGGTC 
CCCTCCCGCA CCTCCCGACC CAAACACCGG CCCGGCCCCA GCAGCACTCG TGAACCCCTG 
CTTAGCAGCT CCGAAAACGG CAATGGCAGC CGAGAGATGC CCCCGGCAGA TGGCCGGATG 
GAGAGACTCT TCAACTCCCT CCTGCCCTTC TGCAGACAAG GCCTCGAGGG CCAGCTCGCC 
GCCAAGGCCC TTCAGAAAGT GCTGACCCTG AAACAGCTCC TGTTGCCTTC GGAGGAAGAC 
AGCGGGGCAG GGCCTCCCCT CGAAGGGGAT GGGGTCCCGG GGGGCAGCCC CTTGAGCCCA 
ACGCAGCCCC AGGAAATTCG GGAGGAGCTG CTCAGCCTGG AGGAGACCAT TAAACAGCTA 
GAGGAGGTGG AGGAGGAATT TTGCCGCCTG AGACTCCTCC TGTCTCAGCT TGGGGAGAAC 
ACCGTCCCCC AGTCTGGCTG TACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARHGEF1 ( XM_010823709.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010822011.1 Bos taurus Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa48603
Clone ID Related Accession (Same CDS sequence)	XM_010815123.2 , XM_010823709.2
Accession Version	XM_010823709.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2787bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	rho guanine nucleotide exchange factor 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646390.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010823709.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGAGA GAGGCGAGAC AGCGAGCGAC ACGGACAGCC CGGCAGTGCC CATGGAGGCA 
GAAGACGTCG CCCGAGGGGC GGCCCCGGGG CCCCCCCGAC CCGGCCTGGC GCCCGTCAGC 
ATCATTGGGG CAGAGGACGA GGATTTTGAG ATTGAGTTGA AGACGAACCC TGAGGAACAA 
AACAACCAGT TCCAGAACCT GGATCAGGTG AAGCGGCGCC CGGCCCACCT CATGGCCCTC 
CTGCAGCACG TGGCCATGCA GTTTGAGCCG GGACCCCTGC TCTGCTGCCT GCATGCGGAC 
ATGCTGGGCT CCCTGGGCCC CAAGGAGTTC AAGAAAGCCT TCATCGACTT CTACCACAGC 
TTCCTGGATA AGCACTCGAT TCTACGAGTG ATGGTCCCTC CTGCCGTGGC CTTTGAACTT 
GACCGCACGC GGCCTGACCT CTTTCCTGAG GATCTCCAGC GGCAGTTCGT GCAGGAGGTG 
GTGCAGAGCC AGCAGGTAGC CGTGGTCCGG CAGCTGGAGG ACTTCCGCTC CAAGAAGCTC 
ATGGGCATGA CACCCTGGGA GCAGGAGCTG GTCCAGCTGG GGGCCTGGGT GGCGCGGGAC 
CGTGCCAGCT ACGAGGCCCG GGAGCGGCAC CTGGCTGAGC GGCAACTGAC CCACCTGGAG 
GAGATGCAGC ACACCATCTC TAACGATGAG GAGAAGAGCG CCGCCGTGGT CAACGCCATC 
AGCATGTACA TGCGGCACCT TGGGGTGCGG ACCAAGAGTG GGGACAAGAA GTCCGGGAGG 
AACTTCTTTC GGAAAAAGAT GATAGGGAAC CGGCGGTCAG ATGAACCGAC CAAGACCAAG 
AAAGGCCTGA GCAGCTTCCT GGATGCCGCC CGCTGGAACC GGGGAGAGCC CCAGGATTTT 
CGACACCCGA AGGTCGAAGT TGATGCTGAG AAGCCAGGCC TTACAGAACG TAAGGGAGGT 
CAGGGGGCGC CTTCCCGGGA CCGGAATCTC GGAGGCCCTG GGCAGGACAC CCCCGGAGTT 
TCTCCGCACC TTCTGTCTGG GGACAGCCCT GACCGGGAGC CAGGTGTTGA CGCCCTCCTG 
GAGGTGGGGG ACCCGCCCCC ACAGGGCCCA GCCAGCCTGG ACGCCCCAGC GCCGCTGGAG 
AGCACGGAGG AGGCTGCCGA CACAGAAAGC CCGGAGCCTG GAGATGAGGG AGAGCCGGGC 
CGCTCAGGAC TGGAGCTGGA ACCAGAGGAG CCGCCTGGCT GGCGAGAGCT TGTCCCCCAG 
GACATTCTGC ACAGCCTGCC CAAGAGCCAG GTGAAGCGGC AGGAGGTCAT CAGCGAGCTC 
CTGGTGACGG AGGCTGCCCA TGTGCGCATG CTCCGCGTGC TCCACGACGT CTTCTACCAG 
CCCATAGCGG ACGGGGGCTT CTACTCCCAG GAGGAGCTCC AGAACATCTT CCCCAGCCTG 
GACGAGCTCA TCGAGGTGCA TTCCCTATTC CTTGATTCCC TGATGAAGCG GAGGCAGGAC 
AGTGGCTACC TCATCAAGGA GATCGGAGAT GTGCTGCTGG CCCGGTTCGA TGGTGCCGAG 
GGCTCGTGGT TCCAGAAAAT CTCCTCCCGC TTCTGTAGCC GCCAGTCGTT CGCCTTAGAG 
CAGCTCAAAG CCAAACAGCG CAAGGAGCCC CGGTTCTGTA CCTTCGTGCA GGAGGCCGAG 
AGCCGGCCCC GGTGCCGCCG GCTGCAGCTG AAGGACATGA TCCCCACCGA GATGCAGCGT 
CTGACCAAGT ACCCACTGCT TCTGCAGAGC ATCGAGCAGA ACACAGAAGA GCCGGTGGAA 
CGGGAGAAAG TGGAGCGGGC AGCCGAGTGC TGCCGGGAAA TTCTGCAACA CGTCAACCTT 
GCCGTGCGGG ACATGGAGGA CCTGCTGCGG CTCAAAGATT ATCAGAGGCG TCTGGATTTG 
ACCCACCTGC GGCAGAGCAA CGACCCCATG CTGAGCGAGT TCAAGAACCT AGACATCACC 
AAGAAGAGGT TGATCTACGA GGGCCCACTG ACGTGGCGGG TGACGAAGGA CAAGGCTGTG 
GAGGTCCACG TGCTGCTGCT GGATGACCTG CTGCTGCTGC TTCAGCGCCA GGACGAGCGG 
TTCTTGCTTA AGTCGCACAG CCGGACGCTG ACGCCCACGC CCGATGGCAA GACCATGCTG 
CGGCCAGTGC TGCGGCTCAC CTCCGCCATG ACCCGCGAGG TGGCCACCGA TCACAAAGCC 
TTCTATGTCA TTTTTACCTG GGACCAGGAG GCCCAGATAT ACGAGCTGGT GGCGCAGACG 
GTGTCTGAGC GGAGGATCTG GTGTACCCTG ATCACCGAGA CCGCTGGATG CCTGAAGGTC 
CCCTCCCGCA CCTCCCGACC CAAACACCGG CCCGGCCCCA GCAGCACTCG TGAACCCCTG 
CTTAGCAGCT CCGAAAACGG CAATGGCAGC CGAGAGATGC CCCCGGCAGA TGGCCGGATG 
GAGAGACTCT TCAACTCCCT CCTGCCCTTC TGCAGACAAG GCCTCGAGGG CCAGCTCGCC 
GCCAAGGCCC TTCAGAAAGT GCTGACCCTG AAACAGCTCC TGTTGCCTTC GGAGGAAGAC 
AGCGGGGCAG GGCCTCCCCT CGAAGGGGAT GGGGTCCCGG GGGGCAGCCC CTTGAGCCCA 
ACGCAGCCCC AGGAAATTCG GGAGGAGCTG CTCAGCCTGG AGGAGACCAT TAAACAGCTA 
GAGGAGGTGG AGGAGGAATT TTGCCGCCTG AGACTCCTCC TGTCTCAGCT TGGGGAGAAC 
ACCGTCCCCC AGTCTGGCTG TACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010822011.1
CDS	138..2924
Translation	MGERGETASDTDSPAVPMEAEDVARGAAPGPPRPGLAPVSIIGAEDEDFEIELKTNPEEQNNQFQNLDQVKRRPAHLMALLQHVAMQFEPGPLLCCLHADMLGSLGPKEFKKAFIDFYHSFLDKHSILRVMVPPAVAFELDRTRPDLFPEDLQRQFVQEVVQSQQVAVVRQLEDFRSKKLMGMTPWEQELVQLGAWVARDRASYEARERHLAERQLTHLEEMQHTISNDEEKSAAVVNAISMYMRHLGVRTKSGDKKSGRNFFRKKMIGNRRSDEPTKTKKGLSSFLDAARWNRGEPQDFRHPKVEVDAEKPGLTERKGGQGAPSRDRNLGGPGQDTPGVSPHLLSGDSPDREPGVDALLEVGDPPPQGPASLDAPAPLESTEEAADTESPEPGDEGEPGRSGLELEPEEPPGWRELVPQDILHSLPKSQVKRQEVISELLVTEAAHVRMLRVLHDVFYQPIADGGFYSQEELQNIFPSLDELIEVHSLFLDSLMKRRQDSGYLIKEIGDVLLARFDGAEGSWFQKISSRFCSRQSFALEQLKAKQRKEPRFCTFVQEAESRPRCRRLQLKDMIPTEMQRLTKYPLLLQSIEQNTEEPVEREKVERAAECCREILQHVNLAVRDMEDLLRLKDYQRRLDLTHLRQSNDPMLSEFKNLDITKKRLIYEGPLTWRVTKDKAVEVHVLLLDDLLLLLQRQDERFLLKSHSRTLTPTPDGKTMLRPVLRLTSAMTREVATDHKAFYVIFTWDQEAQIYELVAQTVSERRIWCTLITETAGCLKVPSRTSRPKHRPGPSSTREPLLSSSENGNGSREMPPADGRMERLFNSLLPFCRQGLEGQLAAKALQKVLTLKQLLLPSEEDSGAGPPLEGDGVPGGSPLSPTQPQEIREELLSLEETIKQLEEVEEEFCRLRLLLSQLGENTVPQSGCT

Target ORF information:

RefSeq Version	XM_010823709.2
Organism	Bos taurus(cattle)
Definition	Bos taurus Rho guanine nucleotide exchange factor 1 (ARHGEF1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010823709.2

ORF Insert Sequence:

ATGGGAGAGA GAGGCGAGAC AGCGAGCGAC ACGGACAGCC CGGCAGTGCC CATGGAGGCA 
GAAGACGTCG CCCGAGGGGC GGCCCCGGGG CCCCCCCGAC CCGGCCTGGC GCCCGTCAGC 
ATCATTGGGG CAGAGGACGA GGATTTTGAG ATTGAGTTGA AGACGAACCC TGAGGAACAA 
AACAACCAGT TCCAGAACCT GGATCAGGTG AAGCGGCGCC CGGCCCACCT CATGGCCCTC 
CTGCAGCACG TGGCCATGCA GTTTGAGCCG GGACCCCTGC TCTGCTGCCT GCATGCGGAC 
ATGCTGGGCT CCCTGGGCCC CAAGGAGTTC AAGAAAGCCT TCATCGACTT CTACCACAGC 
TTCCTGGATA AGCACTCGAT TCTACGAGTG ATGGTCCCTC CTGCCGTGGC CTTTGAACTT 
GACCGCACGC GGCCTGACCT CTTTCCTGAG GATCTCCAGC GGCAGTTCGT GCAGGAGGTG 
GTGCAGAGCC AGCAGGTAGC CGTGGTCCGG CAGCTGGAGG ACTTCCGCTC CAAGAAGCTC 
ATGGGCATGA CACCCTGGGA GCAGGAGCTG GTCCAGCTGG GGGCCTGGGT GGCGCGGGAC 
CGTGCCAGCT ACGAGGCCCG GGAGCGGCAC CTGGCTGAGC GGCAACTGAC CCACCTGGAG 
GAGATGCAGC ACACCATCTC TAACGATGAG GAGAAGAGCG CCGCCGTGGT CAACGCCATC 
AGCATGTACA TGCGGCACCT TGGGGTGCGG ACCAAGAGTG GGGACAAGAA GTCCGGGAGG 
AACTTCTTTC GGAAAAAGAT GATAGGGAAC CGGCGGTCAG ATGAACCGAC CAAGACCAAG 
AAAGGCCTGA GCAGCTTCCT GGATGCCGCC CGCTGGAACC GGGGAGAGCC CCAGGATTTT 
CGACACCCGA AGGTCGAAGT TGATGCTGAG AAGCCAGGCC TTACAGAACG TAAGGGAGGT 
CAGGGGGCGC CTTCCCGGGA CCGGAATCTC GGAGGCCCTG GGCAGGACAC CCCCGGAGTT 
TCTCCGCACC TTCTGTCTGG GGACAGCCCT GACCGGGAGC CAGGTGTTGA CGCCCTCCTG 
GAGGTGGGGG ACCCGCCCCC ACAGGGCCCA GCCAGCCTGG ACGCCCCAGC GCCGCTGGAG 
AGCACGGAGG AGGCTGCCGA CACAGAAAGC CCGGAGCCTG GAGATGAGGG AGAGCCGGGC 
CGCTCAGGAC TGGAGCTGGA ACCAGAGGAG CCGCCTGGCT GGCGAGAGCT TGTCCCCCAG 
GACATTCTGC ACAGCCTGCC CAAGAGCCAG GTGAAGCGGC AGGAGGTCAT CAGCGAGCTC 
CTGGTGACGG AGGCTGCCCA TGTGCGCATG CTCCGCGTGC TCCACGACGT CTTCTACCAG 
CCCATAGCGG ACGGGGGCTT CTACTCCCAG GAGGAGCTCC AGAACATCTT CCCCAGCCTG 
GACGAGCTCA TCGAGGTGCA TTCCCTATTC CTTGATTCCC TGATGAAGCG GAGGCAGGAC 
AGTGGCTACC TCATCAAGGA GATCGGAGAT GTGCTGCTGG CCCGGTTCGA TGGTGCCGAG 
GGCTCGTGGT TCCAGAAAAT CTCCTCCCGC TTCTGTAGCC GCCAGTCGTT CGCCTTAGAG 
CAGCTCAAAG CCAAACAGCG CAAGGAGCCC CGGTTCTGTA CCTTCGTGCA GGAGGCCGAG 
AGCCGGCCCC GGTGCCGCCG GCTGCAGCTG AAGGACATGA TCCCCACCGA GATGCAGCGT 
CTGACCAAGT ACCCACTGCT TCTGCAGAGC ATCGAGCAGA ACACAGAAGA GCCGGTGGAA 
CGGGAGAAAG TGGAGCGGGC AGCCGAGTGC TGCCGGGAAA TTCTGCAACA CGTCAACCTT 
GCCGTGCGGG ACATGGAGGA CCTGCTGCGG CTCAAAGATT ATCAGAGGCG TCTGGATTTG 
ACCCACCTGC GGCAGAGCAA CGACCCCATG CTGAGCGAGT TCAAGAACCT AGACATCACC 
AAGAAGAGGT TGATCTACGA GGGCCCACTG ACGTGGCGGG TGACGAAGGA CAAGGCTGTG 
GAGGTCCACG TGCTGCTGCT GGATGACCTG CTGCTGCTGC TTCAGCGCCA GGACGAGCGG 
TTCTTGCTTA AGTCGCACAG CCGGACGCTG ACGCCCACGC CCGATGGCAA GACCATGCTG 
CGGCCAGTGC TGCGGCTCAC CTCCGCCATG ACCCGCGAGG TGGCCACCGA TCACAAAGCC 
TTCTATGTCA TTTTTACCTG GGACCAGGAG GCCCAGATAT ACGAGCTGGT GGCGCAGACG 
GTGTCTGAGC GGAGGATCTG GTGTACCCTG ATCACCGAGA CCGCTGGATG CCTGAAGGTC 
CCCTCCCGCA CCTCCCGACC CAAACACCGG CCCGGCCCCA GCAGCACTCG TGAACCCCTG 
CTTAGCAGCT CCGAAAACGG CAATGGCAGC CGAGAGATGC CCCCGGCAGA TGGCCGGATG 
GAGAGACTCT TCAACTCCCT CCTGCCCTTC TGCAGACAAG GCCTCGAGGG CCAGCTCGCC 
GCCAAGGCCC TTCAGAAAGT GCTGACCCTG AAACAGCTCC TGTTGCCTTC GGAGGAAGAC 
AGCGGGGCAG GGCCTCCCCT CGAAGGGGAT GGGGTCCCGG GGGGCAGCCC CTTGAGCCCA 
ACGCAGCCCC AGGAAATTCG GGAGGAGCTG CTCAGCCTGG AGGAGACCAT TAAACAGCTA 
GAGGAGGTGG AGGAGGAATT TTGCCGCCTG AGACTCCTCC TGTCTCAGCT TGGGGAGAAC 
ACCGTCCCCC AGTCTGGCTG TACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	ARHGEF1
Entrez Gene ID	513377
Full Name	Rho guanine nucleotide exchange factor 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Xenopus tropicalis (tropical clawed frog)	arhgef1	XP_002941974.2
Canis lupus familiaris (dog)	ARHGEF1	XP_005616572.1
Canis lupus familiaris (dog)	LOC100856005	XP_003640357.2
Pan troglodytes (chimpanzee)	ARHGEF1	XP_003953551.1
Mus musculus (house mouse)	Arhgef1	NP_032514.1
Rattus norvegicus (Norway rat)	Arhgef1	NP_067726.3
Homo sapiens (human)	ARHGEF1	NP_945353.1
Bos taurus (cattle)	ARHGEF1	XP_005219451.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

ARHGEF1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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