Sik1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sik1
Entrez Gene ID	59329
Full Name	salt-inducible kinase 1
Synonyms	Sik,Snf1lk
General protein information	Preferred Names salt-inducible kinase 1 Names serine/threonine-protein kinase SIK1 SIK-1 SNF1-like kinase protein kinase KID2 salt-inducible protein kinase 1 serine/threonine-protein kinase SNF1-like kinase 1 serine/threonine-protein kinase SNF1LK
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 20 Map Location: 20p12
Summary	inhibits CREB binding and transcription of cAMP-responsive element (CRE)-dependent genes Cyp11A and Star [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_021693.2	NP_067725.2	serine/threonine-protein kinase SIK1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SIK1	XP_544912.4
Bos taurus (cattle)	SIK1	XP_002685202.2
Rattus norvegicus (Norway rat)	Sik1	NP_067725.2
Mus musculus (house mouse)	Sik1	NP_034961.2
Gallus gallus (chicken)	SIK1	NP_990013.1
Pan troglodytes (chimpanzee)	SIK1	XP_531484.2
	SIK1	XP_002803160.1

Gene Ontology
Bibliography

Related articles in PubMed

A Novel Role of Salt-Inducible Kinase 1 (SIK1) in the Post-Translational Regulation of Scavenger Receptor Class B Type 1 Activity.
Hu Z, Hu J, Shen WJ, Kraemer FB, Azhar S
Biochemistry54(46)6917-30(2015 Nov)

The adrenergic-regulated CRTC1 and CRTC2 phosphorylation and cellular distribution is independent of endogenous SIK1 in the male rat pinealocyte.
McTague J, Ferguson M, Chik CL, Ho AK
Molecular and cellular endocrinology414156-67(2015 Oct)

Salt-Inducible Kinase 1 Terminates cAMP Signaling by an Evolutionarily Conserved Negative-Feedback Loop in ?-Cells.
Kim MJ, Park SK, Lee JH, Jung CY, Sung DJ, Park JH, Yoon YS, Park J, Park KG, Song DK, Cho H, Kim ST, Koo SH
Diabetes64(9)3189-202(2015 Sep)

Salt-inducible kinase 1 (SIK1) is induced by gastrin and inhibits migration of gastric adenocarcinoma cells.
Selvik LK, Rao S, Steigedal TS, Haltbakk I, Misund K, Bruland T, Prestvik WS, L?greid A, Thommesen L
PloS one9(11)e112485(2014)

The CRTC1-SIK1 pathway regulates entrainment of the circadian clock.
Jagannath A, Butler R, Godinho SI, Couch Y, Brown LA, Vasudevan SR, Flanagan KC, Anthony D, Churchill GC, Wood MJ, Steiner G, Ebeling M, Hossbach M, Wettstein JG, Duffield GE, Gatti S, Hankins MW, Foster RG, Peirson SN
Cell154(5)1100-11(2013 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

role for SIK1 in the positive regulation of selective HDL-CE transport function of SR-B1 and steroidogenesis
Title: A Novel Role of Salt-Inducible Kinase 1 (SIK1) in the Post-Translational Regulation of Scavenger Receptor Class B Type 1 Activity.

Results indicate that overexpressing SIK1 can phosphorylate CRTC1 and CRTC2 in the norepinephrine (NE)-stimulated pinealocytes. Howeve, the endogenous SIK1 does not appear to be the main regulator of their phosphorylation and intracellular localization.
Title: The adrenergic-regulated CRTC1 and CRTC2 phosphorylation and cellular distribution is independent of endogenous SIK1 in the male rat pinealocyte.

SIK1 serves as a part of a self-regulatory circuit to modulate insulin secretion from pancreatic beta-cells by controlling cAMP concentration through modulation of PDE4D activity.
Title: Salt-Inducible Kinase 1 Terminates cAMP Signaling by an Evolutionarily Conserved Negative-Feedback Loop in ?-Cells.

Results show a role of SIK1 in gastrin induced responses and suggest that SIK1 may act as tumour suppressor in gastric adenocarcinoma cells.
Title: Salt-inducible kinase 1 (SIK1) is induced by gastrin and inhibits migration of gastric adenocarcinoma cells.

these data identify a previously unrecognized mechanism by which SIK1 mediates the activation of MEF2-dependent transcription by BDNF.
Title: Role of salt-inducible kinase 1 in the activation of MEF2-dependent transcription by BDNF.

The following Sik1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sik1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa05075	NM_021693.2 Latest version!	Rattus norvegicus salt-inducible kinase 1 (Sik1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$454.30 $649.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sik1 ( NM_021693.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_067725.2 Rattus norvegicus salt-inducible kinase 1 (Sik1), mRNA.

Price & Availability↑

CloneID	ORa05075
Clone ID Related Accession (Same CDS sequence)	NM_021693.2
Accession Version	NM_021693.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2331bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-22
Organism	Rattus norvegicus(Norway rat)
Product	serine/threonine-protein kinase SIK1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB020480.1. On Jun 10, 2007 this sequence version replaced NM_021693.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB020480.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGTGATCA TGTCGGAGTT CAGCGCGGTC CCCACAGGCA CCGGCCAGGG CCAGCAGAAG 
CCCCTCCGGG TGGGCTTTTA CGACGTGGAA CGAACCCTGG GCAAAGGCAA TTTTGCAGTG 
GTTAAGCTGG CGCGGCACCG AGTCACCAAA ACGCAGGTTG CAATTAAAAT AATTGACAAG 
ACACGGTTAG ATTCTAGTAA TCTGGAGAAG ATCTACCGGG AGGTCCAGCT CATGAAACTC 
CTGAACCACC CAAATATTAT CAAGCTTTAC CAGGTTATGG AGACAAAAGA CATGCTGTAC 
ATTGTCACGG AGTTTGCCAA AAATGGAGAA ATGTTCGATT ATCTGACTTC CAACGGGCAC 
TTGAGTGAAA ACGAGGCTAG GAAGAAATTC TGGCAGATCC TGTCAGCCGT GGAGTACTGC 
CACAACCATC ACATCGTCCA CCGGGACCTT AAGACAGAGA ACCTTCTGCT GGACGGCAAC 
ATGGACATCA AGCTGGCAGA TTTTGGATTT GGGAATTTCT ACAAGCCAGG GGAGCCTCTG 
TCTACTTGGT GTGGGAGCCC CCCCTATGCT GCTCCCGAAG TCTTTGAGGG GAAGGAGTAT 
GAAGGTCCCC AGCTGGACAT CTGGAGCCTC GGCGTGGTGC TGTATGTCCT GGTCTGTGGC 
TCCCTCCCTT TTGATGGACC CAACCTGCCT ACGCTGAGAC AGCGGGTGCT GGAGGGCCGC 
TTCCGCATCC CCTTCTTCAT GTCTCAAGAC TGTGAGACAC TGATCAGACG CATGCTGGTA 
GTGGACCCCG CCAAGCGCAT CACCATTGCC CAGATCCGCC AGCACCGATG GATGCAGGCC 
GACCCCACCC TCCTGCAGCA GGACGACCCT GCCTTCTCCA TGCAGGGCTA CACCTCCAAC 
CTGGGCGACT ACAACGAGCA GGTGCTGGGC ATCATGCAGG CCCTGGGCAT CGACCGGCAG 
AGGACAGTAG AGTCTCTACA GAACAGCAGC TACAACCACT TTGCGGCTAT TTACTACCTC 
CTGCTCGAGC GCCTCAGGGA GCATCGAAGC ACCCAGCCCT CATCCCGGGC CACCCCTGCA 
CCTGCCAGAC AGCCTCAGCT CCGAAACTCA GACCTCAGCA GTCTGGAGGT TCCTCAAGAA 
ATTCTCCCAT GTGACCCTTT CCGGCCCTCT CTGCTGTGCC CGCAGCCCCA GGCCTTGGCT 
CAGTCTGTCC TGCAGGCTGA GATAGACTGT GATCTCCACA GTTCGCTTCA GCCCTTATTC 
TTCCCCCTGG ATACCAACTG CAGCGGAGTG TTCCGGCACC GATCCATCTC CCCCAGCAGC 
CTGCTGGACA CAGCTATCAG TGAGGAGGCC AGGCAGGGTC CCAGCCTGGA GGAGGAGCAG 
GAGGTCCAGG AACCCCTGCC TGGCAGCACT GGCCGGAGGC ATACACTGGC TGAAGTTTCC 
ACCCACTTCT CCCCGCTCAA CCCTCCTTGC ATAATTGTCT CCTCCTCTGC CGCTGTGAGT 
CCCTCGGAAG GAACTAGCTC TGACAGCTGC CTCCCCTTCT CTGCAAGCGA AGGTCCTGCA 
GGGCTTGGTG GTGGCCTGGC CACCCCAGGG CTTCTGGGCA CCAGCTCTCC AGTCAGATTG 
GCCTCACCCT TCCTGGGATC ACAGTCAGCC ACCCCTGTGC TACAGAGTCA GGCAGGTCTG 
GGTGCGACTG TCTTACCTCC TGTCAGCTTC CAGGAGGGAC GGAGAGCGTC GGATACGTCT 
CTCACTCAAG GGCTGAAGGC CTTCCGGCAG CAGCTAAGGA AAAACGCGAG GACCAAGGGG 
TTCCTGGGAC TGAACAAGAT CAAAGGATTG GCCCGTCAGG TGTGCCAGTC CTCCATCCGA 
GGTTCCCGGG GAGGGATGAG TACCTTCCAC ACCCCGGCCC CAAGCTCAGG TCTGCAAGGC 
TGCACGGCCA GCAGTCGCGA GGGCAGGAGC CTGCTCGAAG AGGTGCTGCA CCAGCAGAGG 
CTGCTCCAGT TACAGCACCA CTCAGCCGTC TCATCTGACT ACCAGCAGGC CCCCCAACTG 
TCCCCCGTCC CCTACGTGCT CACTCCCTGT GATGGCCTTC TCGTGTCTGG AATCCCACTG 
CTGCCAACGC CCCTCCTCCA GCCCGGCATG TCCCCCGTGG CGTCTGCCGC GCAGCTCCTG 
GATGCCCACT TGCACATCAG TGCTGGCCCA GTGGCCCTCC CCACTGGTCC CCTGCCACAG 
TGCCTCACCA GGCTGTCCCC AAGCTGTGAT CCTGCTGGGC TGCCGCAGGG GGATTGTGAG 
ATGGAGGACC TGACCTCTGG CCAGCGGGGG ACGTTCGTCC TGGTACAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_067725.2
CDS	72..2402
Translation	MVIMSEFSAVPTGTGQGQQKPLRVGFYDVERTLGKGNFAVVKLARHRVTKTQVAIKIIDKTRLDSSNLEKIYREVQLMKLLNHPNIIKLYQVMETKDMLYIVTEFAKNGEMFDYLTSNGHLSENEARKKFWQILSAVEYCHNHHIVHRDLKTENLLLDGNMDIKLADFGFGNFYKPGEPLSTWCGSPPYAAPEVFEGKEYEGPQLDIWSLGVVLYVLVCGSLPFDGPNLPTLRQRVLEGRFRIPFFMSQDCETLIRRMLVVDPAKRITIAQIRQHRWMQADPTLLQQDDPAFSMQGYTSNLGDYNEQVLGIMQALGIDRQRTVESLQNSSYNHFAAIYYLLLERLREHRSTQPSSRATPAPARQPQLRNSDLSSLEVPQEILPCDPFRPSLLCPQPQALAQSVLQAEIDCDLHSSLQPLFFPLDTNCSGVFRHRSISPSSLLDTAISEEARQGPSLEEEQEVQEPLPGSTGRRHTLAEVSTHFSPLNPPCIIVSSSAAVSPSEGTSSDSCLPFSASEGPAGLGGGLATPGLLGTSSPVRLASPFLGSQSATPVLQSQAGLGATVLPPVSFQEGRRASDTSLTQGLKAFRQQLRKNARTKGFLGLNKIKGLARQVCQSSIRGSRGGMSTFHTPAPSSGLQGCTASSREGRSLLEEVLHQQRLLQLQHHSAVSSDYQQAPQLSPVPYVLTPCDGLLVSGIPLLPTPLLQPGMSPVASAAQLLDAHLHISAGPVALPTGPLPQCLTRLSPSCDPAGLPQGDCEMEDLTSGQRGTFVLVQ

Target ORF information:

RefSeq Version	NM_021693.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus salt-inducible kinase 1 (Sik1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021693.2

ORF Insert Sequence:

ATGGTGATCA TGTCGGAGTT CAGCGCGGTC CCCACAGGCA CCGGCCAGGG CCAGCAGAAG 
CCCCTCCGGG TGGGCTTTTA CGACGTGGAA CGAACCCTGG GCAAAGGCAA TTTTGCAGTG 
GTTAAGCTGG CGCGGCACCG AGTCACCAAA ACGCAGGTTG CAATTAAAAT AATTGACAAG 
ACACGGTTAG ATTCTAGTAA TCTGGAGAAG ATCTACCGGG AGGTCCAGCT CATGAAACTC 
CTGAACCACC CAAATATTAT CAAGCTTTAC CAGGTTATGG AGACAAAAGA CATGCTGTAC 
ATTGTCACGG AGTTTGCCAA AAATGGAGAA ATGTTCGATT ATCTGACTTC CAACGGGCAC 
TTGAGTGAAA ACGAGGCTAG GAAGAAATTC TGGCAGATCC TGTCAGCCGT GGAGTACTGC 
CACAACCATC ACATCGTCCA CCGGGACCTT AAGACAGAGA ACCTTCTGCT GGACGGCAAC 
ATGGACATCA AGCTGGCAGA TTTTGGATTT GGGAATTTCT ACAAGCCAGG GGAGCCTCTG 
TCTACTTGGT GTGGGAGCCC CCCCTATGCT GCTCCCGAAG TCTTTGAGGG GAAGGAGTAT 
GAAGGTCCCC AGCTGGACAT CTGGAGCCTC GGCGTGGTGC TGTATGTCCT GGTCTGTGGC 
TCCCTCCCTT TTGATGGACC CAACCTGCCT ACGCTGAGAC AGCGGGTGCT GGAGGGCCGC 
TTCCGCATCC CCTTCTTCAT GTCTCAAGAC TGTGAGACAC TGATCAGACG CATGCTGGTA 
GTGGACCCCG CCAAGCGCAT CACCATTGCC CAGATCCGCC AGCACCGATG GATGCAGGCC 
GACCCCACCC TCCTGCAGCA GGACGACCCT GCCTTCTCCA TGCAGGGCTA CACCTCCAAC 
CTGGGCGACT ACAACGAGCA GGTGCTGGGC ATCATGCAGG CCCTGGGCAT CGACCGGCAG 
AGGACAGTAG AGTCTCTACA GAACAGCAGC TACAACCACT TTGCGGCTAT TTACTACCTC 
CTGCTCGAGC GCCTCAGGGA GCATCGAAGC ACCCAGCCCT CATCCCGGGC CACCCCTGCA 
CCTGCCAGAC AGCCTCAGCT CCGAAACTCA GACCTCAGCA GTCTGGAGGT TCCTCAAGAA 
ATTCTCCCAT GTGACCCTTT CCGGCCCTCT CTGCTGTGCC CGCAGCCCCA GGCCTTGGCT 
CAGTCTGTCC TGCAGGCTGA GATAGACTGT GATCTCCACA GTTCGCTTCA GCCCTTATTC 
TTCCCCCTGG ATACCAACTG CAGCGGAGTG TTCCGGCACC GATCCATCTC CCCCAGCAGC 
CTGCTGGACA CAGCTATCAG TGAGGAGGCC AGGCAGGGTC CCAGCCTGGA GGAGGAGCAG 
GAGGTCCAGG AACCCCTGCC TGGCAGCACT GGCCGGAGGC ATACACTGGC TGAAGTTTCC 
ACCCACTTCT CCCCGCTCAA CCCTCCTTGC ATAATTGTCT CCTCCTCTGC CGCTGTGAGT 
CCCTCGGAAG GAACTAGCTC TGACAGCTGC CTCCCCTTCT CTGCAAGCGA AGGTCCTGCA 
GGGCTTGGTG GTGGCCTGGC CACCCCAGGG CTTCTGGGCA CCAGCTCTCC AGTCAGATTG 
GCCTCACCCT TCCTGGGATC ACAGTCAGCC ACCCCTGTGC TACAGAGTCA GGCAGGTCTG 
GGTGCGACTG TCTTACCTCC TGTCAGCTTC CAGGAGGGAC GGAGAGCGTC GGATACGTCT 
CTCACTCAAG GGCTGAAGGC CTTCCGGCAG CAGCTAAGGA AAAACGCGAG GACCAAGGGG 
TTCCTGGGAC TGAACAAGAT CAAAGGATTG GCCCGTCAGG TGTGCCAGTC CTCCATCCGA 
GGTTCCCGGG GAGGGATGAG TACCTTCCAC ACCCCGGCCC CAAGCTCAGG TCTGCAAGGC 
TGCACGGCCA GCAGTCGCGA GGGCAGGAGC CTGCTCGAAG AGGTGCTGCA CCAGCAGAGG 
CTGCTCCAGT TACAGCACCA CTCAGCCGTC TCATCTGACT ACCAGCAGGC CCCCCAACTG 
TCCCCCGTCC CCTACGTGCT CACTCCCTGT GATGGCCTTC TCGTGTCTGG AATCCCACTG 
CTGCCAACGC CCCTCCTCCA GCCCGGCATG TCCCCCGTGG CGTCTGCCGC GCAGCTCCTG 
GATGCCCACT TGCACATCAG TGCTGGCCCA GTGGCCCTCC CCACTGGTCC CCTGCCACAG 
TGCCTCACCA GGCTGTCCCC AAGCTGTGAT CCTGCTGGGC TGCCGCAGGG GGATTGTGAG 
ATGGAGGACC TGACCTCTGG CCAGCGGGGG ACGTTCGTCC TGGTACAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A Novel Role of Salt-Inducible Kinase 1 (SIK1) in the Post-Translational Regulation of Scavenger Receptor Class B Type 1 Activity.
Biochemistry54(46)6917-30(2015 Nov)
Hu Z,Hu J,Shen WJ,Kraemer FB,Azhar S

The adrenergic-regulated CRTC1 and CRTC2 phosphorylation and cellular distribution is independent of endogenous SIK1 in the male rat pinealocyte.
Molecular and cellular endocrinology414156-67(2015 Oct)
McTague J,Ferguson M,Chik CL,Ho AK

Salt-Inducible Kinase 1 Terminates cAMP Signaling by an Evolutionarily Conserved Negative-Feedback Loop in ?-Cells.
Diabetes64(9)3189-202(2015 Sep)
Kim MJ,Park SK,Lee JH,Jung CY,Sung DJ,Park JH,Yoon YS,Park J,Park KG,Song DK,Cho H,Kim ST,Koo SH

Salt-inducible kinase 1 (SIK1) is induced by gastrin and inhibits migration of gastric adenocarcinoma cells.
PloS one9(11)e112485(2014)
Selvik LK,Rao S,Steigedal TS,Haltbakk I,Misund K,Bruland T,Prestvik WS,L?greid A,Thommesen L

The CRTC1-SIK1 pathway regulates entrainment of the circadian clock.
Cell154(5)1100-11(2013 Aug)
Jagannath A,Butler R,Godinho SI,Couch Y,Brown LA,Vasudevan SR,Flanagan KC,Anthony D,Churchill GC,Wood MJ,Steiner G,Ebeling M,Hossbach M,Wettstein JG,Duffield GE,Gatti S,Hankins MW,Foster RG,Peirson SN

- Most Popular Services
  - 人工遺伝子合成& DNA合成-納期保証
- Services & Products

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

Sik1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報