The following Mid1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mid1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | ORa13887 | |
| Clone ID Related Accession (Same CDS sequence) | NM_022927.1 | |
| Accession Version | NM_022927.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-09-22 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | E3 ubiquitin-protein ligase Midline-1 | |
| Comment | Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF186461.1. ##Evidence-Data-START## Transcript exon combination :: AF186461.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689598, SAMEA2689600 [ECO:0000348] ##Evidence-Data-END## | |
1 | ATGGAAACAC TGGAGTCGGA GCTGACCTGT CCTATTTGTC TGGAGCTTTT TGAGGACCCT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_075216.1 |
| CDS | 233..2236 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_022927.1 |
1 | ATGGAAACAC TGGAGTCGGA GCTGACCTGT CCTATTTGTC TGGAGCTTTT TGAGGACCCT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ORa39661 | |
| Clone ID Related Accession (Same CDS sequence) | XM_017602143.1 , XM_006256825.3 , XM_017602142.1 | |
| Accession Version | XM_017602143.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-07-25 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | E3 ubiquitin-protein ligase Midline-1 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGAAACAC TGGAGTCGGA GCTGACCTGT CCTATTTGTC TGGAGCTTTT TGAGGACCCT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_017457632.1 |
| CDS | 765..2768 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_017602143.1 |
1 | ATGGAAACAC TGGAGTCGGA GCTGACCTGT CCTATTTGTC TGGAGCTTTT TGAGGACCCT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ORa39661 | |
| Clone ID Related Accession (Same CDS sequence) | XM_017602143.1 , XM_006256825.3 , XM_017602142.1 | |
| Accession Version | XM_006256825.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-07-25 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | E3 ubiquitin-protein ligase Midline-1 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006256825.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGAAACAC TGGAGTCGGA GCTGACCTGT CCTATTTGTC TGGAGCTTTT TGAGGACCCT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_006256887.1 |
| CDS | 1510..3513 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_006256825.3 |
1 | ATGGAAACAC TGGAGTCGGA GCTGACCTGT CCTATTTGTC TGGAGCTTTT TGAGGACCCT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ORa39661 | |
| Clone ID Related Accession (Same CDS sequence) | XM_017602143.1 , XM_006256825.3 , XM_017602142.1 | |
| Accession Version | XM_017602142.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-07-25 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | E3 ubiquitin-protein ligase Midline-1 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGAAACAC TGGAGTCGGA GCTGACCTGT CCTATTTGTC TGGAGCTTTT TGAGGACCCT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_017457631.1 |
| CDS | 378..2381 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_017602142.1 |
1 | ATGGAAACAC TGGAGTCGGA GCTGACCTGT CCTATTTGTC TGGAGCTTTT TGAGGACCCT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Systematic analysis of dimeric E3-RING interactions reveals increased combinatorial complexity in human ubiquitination networks. |
TRIM27 negatively regulates NOD2 by ubiquitination and proteasomal degradation. |
Active transport of the ubiquitin ligase MID1 along the microtubules is regulated by protein phosphatase 2A. |
Alternative polyadenylation signals and promoters act in concert to control tissue-specific expression of the Opitz Syndrome gene MID1. |
Cytokine activation of p38 mitogen-activated protein kinase and apoptosis is opposed by alpha-4 targeting of protein phosphatase 2A for site-specific dephosphorylation of MEK3. |
