Brsk1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Brsk1
Entrez Gene ID	499073
Full Name	BR serine/threonine kinase 1
Synonyms	RGD1563268,Sad-b
General protein information	Preferred Names BR serine/threonine kinase 1 Names serine/threonine-protein kinase BRSK1 BR serine/threonine-protein kinase 1 brain-specific serine/threonine-protein kinase 1 serine/threonine-protein kinase SAD-B
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q12

mRNA and Protein(s)

mRNA	Protein	Name
NM_001127337.1	NP_001120809.1	serine/threonine-protein kinase BRSK1
XM_006228283.2	XP_006228345.1	serine/threonine-protein kinase BRSK1 isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	BRSK1	XP_541413.3
Rattus norvegicus (Norway rat)	Brsk1	NP_001120809.1
Danio rerio (zebrafish)	LOC558390	XP_003198179.2
Bos taurus (cattle)	BRSK1	XP_002695456.3
Xenopus tropicalis (tropical clawed frog)	brsk1	XP_004916365.1
Homo sapiens (human)	BRSK1	NP_115806.1
Mus musculus (house mouse)	Brsk1	NP_001003920.2
Danio rerio (zebrafish)	si:ch211-255p10.4	XP_693123.4

Gene Ontology
Bibliography

Related articles in PubMed

SAD-B kinase regulates pre-synaptic vesicular dynamics at hippocampal Schaffer collateral synapses and affects contextual fear memory.
Watabe AM, Nagase M, Hagiwara A, Hida Y, Tsuji M, Ochiai T, Kato F, Ohtsuka T
Journal of neurochemistry136(1)36-47(2016 Jan)

Polarized activities of AMPK and BRSK in primary hippocampal neurons.
Sample V, Ramamurthy S, Gorshkov K, Ronnett GV, Zhang J
Molecular biology of the cell26(10)1935-46(2015 May)

Phosphorylation of microtubule-associated protein tau by AMPK-related kinases.
Yoshida H, Goedert M
Journal of neurochemistry120(1)165-76(2012 Jan)

Persistence of the cell-cycle checkpoint kinase Wee1 in SadA- and SadB-deficient neurons disrupts neuronal polarity.
M?ller M, Lutter D, P?schel AW
Journal of cell science123(Pt 2)286-94(2010 Jan)

SADB phosphorylation of gamma-tubulin regulates centrosome duplication.
Alvarado-Kristensson M, Rodr?guez MJ, Sili? V, Valpuesta JM, Carrera AC
Nature cell biology11(9)1081-92(2009 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The activities of both AMPK and BRSK are polarized in developing hippocampal neurons, with high levels in the distal region of extended axons.
Title: Polarized activities of AMPK and BRSK in primary hippocampal neurons.

The regulation of Wee1 by SadA and SadB kinases is essential for the differentiation of polarized neurons.
Title: Persistence of the cell-cycle checkpoint kinase Wee1 in SadA- and SadB-deficient neurons disrupts neuronal polarity.

The following Brsk1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Brsk1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa01573	NM_001127337.1 Latest version!	Rattus norvegicus BR serine/threonine kinase 1 (Brsk1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$461.30 $659.00
ORa22334	XM_006228283.2 Latest version!	Rattus norvegicus BR serine/threonine kinase 1 (Brsk1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Brsk1 ( NM_001127337.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001120809.1 Rattus norvegicus BR serine/threonine kinase 1 (Brsk1), mRNA.

Price & Availability↑

CloneID	ORa01573
Clone ID Related Accession (Same CDS sequence)	NM_001127337.1
Accession Version	NM_001127337.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2337bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Rattus norvegicus(Norway rat)
Product	serine/threonine-protein kinase BRSK1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB365521.1. On or before May 14, 2008 this sequence version replaced XM_001071463.1, XM_574355.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB365521.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGTCGTCCG GGTCCAAGGA AGGTGGCGGG GGCTCCCCCG CCTACCACCT CCCACACCCA 
CACCCACACC CACCCCAGCA CGCCCAATAT GTGGGCCCCT ATCGGCTGGA GAAGACGCTG 
GGCAAAGGAC AGACAGGTCT AGTTAAACTT GGGGTCCACT GCATCACGGG TCAGAAGGTC 
GCTGTCAAGA TCGTGAACAG GGAGAAGCTG TCGGAATCTG TGCTGATGAA GGTGGAGAGG 
GAAATCGCCA TTCTAAAGCT CATTGAACAC CCGCATGTGC TCAAGCTCCA CGACGTCTAC 
GAGAACAAGA AATATTTATA CTTGGTGCTT GAGCATGTTT CTGGTGGTGA GCTGTTCGAC 
TACCTGGTAA AAAAAGGGAG ACTGACACCC AAGGAGGCCC GAAAGTTCTT CCGCCAGATT 
GTGTCAGCGC TGGACTTCTG CCATAGCTAC TCCATCTGTC ACAGGGACCT GAAGCCAGAG 
AACCTGCTGC TGGATGAGAA AAACAACATC CGCATCGCAG ACTTTGGCAT GGCGTCCCTG 
CAGGTGGGGG ACAGCCTCCT GGAGACCAGC TGTGGGTCCC CCCATTACGC ATGTCCAGAG 
GTGATCAAGG GGGAGAAGTA TGATGGCCGC CGGGCAGACA TGTGGAGCTG TGGAGTCATC 
CTGTTTGCCC TGCTTGTGGG GGCGCTGCCC TTCGATGATG ACAACCTACG ACAGCTACTA 
GAGAAGGTGA AACGTGGTGT CTTCCACATG CCGCACTTCA TCCCTCCAGA CTGCCAGAGC 
CTCCTGAGAG GGATGATTGA AGTGGAGCCC GAGAAAAGGC TCAGTCTGGA GCAAATTCAG 
AAACATCCTT GGTATCTGGG CGGGAAACAC GAACCAGACC CTTGCCTGGA GCCAGCCCCA 
GGCCGCAGAG TAGCTATGCG GAGCCTGCCT TCCAATGGCG AGCTGGACCC TGACGTTCTG 
GAAAGCATGG CGTCTCTGGG CTGCTTCAGA GACCGCGAGC GGCTACACAG AGAACTGCGA 
AGTGAGGAGG AAAACCAAGA AAAGATGATC TATTATTTGC TTTTGGATCG GAAAGAGCGG 
TACCCTAGCT GTGAAGACCA GGACCTGCCT CCTCGGAATG ATGTTGACCC ACCTCGGAAG 
CGTGTGGATT CCCCCATGCT GAGCAGACAC GGGAAACGGA GGCCAGAGCG GAAGTCCATG 
GAAGTCCTCA GCATCACAGA TGCTGGGAGT GGTGGCTCTC CAGTGCCTAC CAGAAGGGCC 
TTGGAGATGG CCCAGCACAG TCAGAGATCC CGCAGCGTCA GTGGAGCATC CACTGGTCTG 
TCCTCCAGCC CTCTAAGCAG CCCAAGGAGT CCGGTCTTTT CCTTCTCACC GGAGCCAGGC 
GTTGGAGATG AGGCTAGAGG TGGAGGCTCC CCAACTTCCA AAACACAGAC GCTGCCTTCT 
CGGGGCCCCA GAGGTGGGGG CGCTGGAGAG CAGCCACCAC CTCCCAGTGC CCGTTCCACG 
CCCTTGCCTG GACCACCAGG CTCCCCGCGC TCCTCCGGCG GAACCCCCTT GCACTCACCT 
CTGCACACGC CTCGAGCCAG CCCTACCGGG ACTCCAGGAA CTACACCACC TCCCAGCCCA 
GGCGGTGGCG TCGGGGGAGC CGCCTGGAGA AGTCGTCTCA ACTCCATCCG CAACAGCTTC 
CTGGGCTCCC CTCGCTTCCA CCGGCGCAAG ATGCAGGTCC CTACCGCTGA GGAGATGTCC 
AGTTTGACAC CAGAATCCTC TCCAGAGCTG GCAAAACGCT CCTGGTTCGG GAATTTCATC 
TCCTTGGACA AAGAAGAACA AATATTCCTC GTGCTAAAGG ACAAACCTCT CAGCAGCATC 
AAAGCGGACA TTGTCCATGC CTTTCTGTCG ATCCCTAGCC TGAGTCACAG TGTGCTGTCA 
CAGACCAGCT TCAGGGCCGA ATACAAGGCC AGCGGTGGCC CCTCTGTCTT CCAGAAGCCT 
GTCCGCTTCC AGGTGGACAT CAGCTCCTCT GAGGGTCCAG AACCCTCACC CCGAAGAGAT 
GGTAGCAGTG GAGGTGGCAT CTACTCAGTC ACCTTCACTC TCATCTCCGG TCCTAGCCGT 
CGGTTCAAGC GTGTGGTAGA GACCATCCAG GCACAGTTGC TGAGTACTCA TGACCAGCCC 
TCCGTGCAGG CCTTGGCAGA TGAGAAGAAT GGAGCCCAGA CCCGGCCTGC TGGTACCCCA 
CCCCGAAGCC TGCAGCCTCC ACCAGGCCGC CCTGACCCTG ATCTGAGTAG CTCTCCCCGC 
CGAGGACCCT CTAAGGACAA GAAGCTCCTG GCCACCAATG GAACCCCTCT ACCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001120809.1
CDS	1..2337
Translation	MSSGSKEGGGGSPAYHLPHPHPHPPQHAQYVGPYRLEKTLGKGQTGLVKLGVHCITGQKVAVKIVNREKLSESVLMKVEREIAILKLIEHPHVLKLHDVYENKKYLYLVLEHVSGGELFDYLVKKGRLTPKEARKFFRQIVSALDFCHSYSICHRDLKPENLLLDEKNNIRIADFGMASLQVGDSLLETSCGSPHYACPEVIKGEKYDGRRADMWSCGVILFALLVGALPFDDDNLRQLLEKVKRGVFHMPHFIPPDCQSLLRGMIEVEPEKRLSLEQIQKHPWYLGGKHEPDPCLEPAPGRRVAMRSLPSNGELDPDVLESMASLGCFRDRERLHRELRSEEENQEKMIYYLLLDRKERYPSCEDQDLPPRNDVDPPRKRVDSPMLSRHGKRRPERKSMEVLSITDAGSGGSPVPTRRALEMAQHSQRSRSVSGASTGLSSSPLSSPRSPVFSFSPEPGVGDEARGGGSPTSKTQTLPSRGPRGGGAGEQPPPPSARSTPLPGPPGSPRSSGGTPLHSPLHTPRASPTGTPGTTPPPSPGGGVGGAAWRSRLNSIRNSFLGSPRFHRRKMQVPTAEEMSSLTPESSPELAKRSWFGNFISLDKEEQIFLVLKDKPLSSIKADIVHAFLSIPSLSHSVLSQTSFRAEYKASGGPSVFQKPVRFQVDISSSEGPEPSPRRDGSSGGGIYSVTFTLISGPSRRFKRVVETIQAQLLSTHDQPSVQALADEKNGAQTRPAGTPPRSLQPPPGRPDPDLSSSPRRGPSKDKKLLATNGTPLP

Target ORF information:

RefSeq Version	NM_001127337.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus BR serine/threonine kinase 1 (Brsk1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001127337.1

ORF Insert Sequence:

ATGTCGTCCG GGTCCAAGGA AGGTGGCGGG GGCTCCCCCG CCTACCACCT CCCACACCCA 
CACCCACACC CACCCCAGCA CGCCCAATAT GTGGGCCCCT ATCGGCTGGA GAAGACGCTG 
GGCAAAGGAC AGACAGGTCT AGTTAAACTT GGGGTCCACT GCATCACGGG TCAGAAGGTC 
GCTGTCAAGA TCGTGAACAG GGAGAAGCTG TCGGAATCTG TGCTGATGAA GGTGGAGAGG 
GAAATCGCCA TTCTAAAGCT CATTGAACAC CCGCATGTGC TCAAGCTCCA CGACGTCTAC 
GAGAACAAGA AATATTTATA CTTGGTGCTT GAGCATGTTT CTGGTGGTGA GCTGTTCGAC 
TACCTGGTAA AAAAAGGGAG ACTGACACCC AAGGAGGCCC GAAAGTTCTT CCGCCAGATT 
GTGTCAGCGC TGGACTTCTG CCATAGCTAC TCCATCTGTC ACAGGGACCT GAAGCCAGAG 
AACCTGCTGC TGGATGAGAA AAACAACATC CGCATCGCAG ACTTTGGCAT GGCGTCCCTG 
CAGGTGGGGG ACAGCCTCCT GGAGACCAGC TGTGGGTCCC CCCATTACGC ATGTCCAGAG 
GTGATCAAGG GGGAGAAGTA TGATGGCCGC CGGGCAGACA TGTGGAGCTG TGGAGTCATC 
CTGTTTGCCC TGCTTGTGGG GGCGCTGCCC TTCGATGATG ACAACCTACG ACAGCTACTA 
GAGAAGGTGA AACGTGGTGT CTTCCACATG CCGCACTTCA TCCCTCCAGA CTGCCAGAGC 
CTCCTGAGAG GGATGATTGA AGTGGAGCCC GAGAAAAGGC TCAGTCTGGA GCAAATTCAG 
AAACATCCTT GGTATCTGGG CGGGAAACAC GAACCAGACC CTTGCCTGGA GCCAGCCCCA 
GGCCGCAGAG TAGCTATGCG GAGCCTGCCT TCCAATGGCG AGCTGGACCC TGACGTTCTG 
GAAAGCATGG CGTCTCTGGG CTGCTTCAGA GACCGCGAGC GGCTACACAG AGAACTGCGA 
AGTGAGGAGG AAAACCAAGA AAAGATGATC TATTATTTGC TTTTGGATCG GAAAGAGCGG 
TACCCTAGCT GTGAAGACCA GGACCTGCCT CCTCGGAATG ATGTTGACCC ACCTCGGAAG 
CGTGTGGATT CCCCCATGCT GAGCAGACAC GGGAAACGGA GGCCAGAGCG GAAGTCCATG 
GAAGTCCTCA GCATCACAGA TGCTGGGAGT GGTGGCTCTC CAGTGCCTAC CAGAAGGGCC 
TTGGAGATGG CCCAGCACAG TCAGAGATCC CGCAGCGTCA GTGGAGCATC CACTGGTCTG 
TCCTCCAGCC CTCTAAGCAG CCCAAGGAGT CCGGTCTTTT CCTTCTCACC GGAGCCAGGC 
GTTGGAGATG AGGCTAGAGG TGGAGGCTCC CCAACTTCCA AAACACAGAC GCTGCCTTCT 
CGGGGCCCCA GAGGTGGGGG CGCTGGAGAG CAGCCACCAC CTCCCAGTGC CCGTTCCACG 
CCCTTGCCTG GACCACCAGG CTCCCCGCGC TCCTCCGGCG GAACCCCCTT GCACTCACCT 
CTGCACACGC CTCGAGCCAG CCCTACCGGG ACTCCAGGAA CTACACCACC TCCCAGCCCA 
GGCGGTGGCG TCGGGGGAGC CGCCTGGAGA AGTCGTCTCA ACTCCATCCG CAACAGCTTC 
CTGGGCTCCC CTCGCTTCCA CCGGCGCAAG ATGCAGGTCC CTACCGCTGA GGAGATGTCC 
AGTTTGACAC CAGAATCCTC TCCAGAGCTG GCAAAACGCT CCTGGTTCGG GAATTTCATC 
TCCTTGGACA AAGAAGAACA AATATTCCTC GTGCTAAAGG ACAAACCTCT CAGCAGCATC 
AAAGCGGACA TTGTCCATGC CTTTCTGTCG ATCCCTAGCC TGAGTCACAG TGTGCTGTCA 
CAGACCAGCT TCAGGGCCGA ATACAAGGCC AGCGGTGGCC CCTCTGTCTT CCAGAAGCCT 
GTCCGCTTCC AGGTGGACAT CAGCTCCTCT GAGGGTCCAG AACCCTCACC CCGAAGAGAT 
GGTAGCAGTG GAGGTGGCAT CTACTCAGTC ACCTTCACTC TCATCTCCGG TCCTAGCCGT 
CGGTTCAAGC GTGTGGTAGA GACCATCCAG GCACAGTTGC TGAGTACTCA TGACCAGCCC 
TCCGTGCAGG CCTTGGCAGA TGAGAAGAAT GGAGCCCAGA CCCGGCCTGC TGGTACCCCA 
CCCCGAAGCC TGCAGCCTCC ACCAGGCCGC CCTGACCCTG ATCTGAGTAG CTCTCCCCGC 
CGAGGACCCT CTAAGGACAA GAAGCTCCTG GCCACCAATG GAACCCCTCT ACCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brsk1 ( XM_006228283.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006228345.1 Rattus norvegicus BR serine/threonine kinase 1 (Brsk1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa22334
Clone ID Related Accession (Same CDS sequence)	XM_006228283.2
Accession Version	XM_006228283.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2067bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	serine/threonine-protein kinase BRSK1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006228283.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCTCAA GCTCCACGAC GTCTACGAGA ACAAGAAATA TTTGTAGATA CTTGGTGCTT 
GAGCATGTTT CTGGTGGTGA GCTGTTCGAC TACCTGGTAA AAAAAGGGAG ACTGACACCC 
AAGGAGGCCC GAAAGTTCTT CCGCCAGATT GTGTCAGCGC TGGACTTCTG CCATAGCTAC 
TCCATCTGTC ACAGGGACCT GAAGCCAGAG AACCTGCTGC TGGATGAGAA AAACAACATC 
CGCATCGCAG ACTTTGGCAT GGCGTCCCTG CAGGTGGGGG ACAGCCTCCT GGAGACCAGC 
TGTGGGTCCC CCCATTACGC ATGTCCAGAG GTGATCAAGG GGGAGAAGTA TGATGGCCGC 
CGGGCAGACA TGTGGAGCTG TGGAGTCATC CTGTTTGCCC TGCTTGTGGG GGCGCTGCCC 
TTCGATGATG ACAACCTACG ACAGCTACTA GAGAAGGTGA AACGTGGTGT CTTCCACATG 
CCGCACTTCA TCCCTCCAGA CTGCCAGAGC CTCCTGAGAG GGATGATTGA AGTGGAGCCC 
GAGAAAAGGC TCAGTCTGGA GCAAATTCAG AAACATCCTT GGTATCTGGG CGGGAAACAC 
GAACCAGACC CTTGCCTGGA GCCAGCCCCA GGCCGCAGAG TAGCTATGCG GAGCCTGCCT 
TCCAATGGCG AGCTGGACCC TGACGTTCTG GAAAGCATGG CGTCTCTGGG CTGCTTCAGA 
GACCGCGAGC GGCTACACAG AGAACTGCGA AGTGAGGAGG AAAACCAAGA AAAGATGATC 
TATTATTTGC TTTTGGATCG GAAAGAGCGG TACCCTAGCT GTGAAGACCA GGACCTGCCT 
CCTCGGAATG ATGTTGACCC ACCTCGGAAG CGTGTGGATT CCCCCATGCT GAGCAGACAC 
GGGAAACGGA GGCCAGAGCG GAAGTCCATG GAAGTCCTCA GCATCACAGA TGCTGGGAGT 
GGTGGCTCTC CAGTGCCTAC CAGAAGGGCC TTGGAGATGG CCCAGCACAG TCAGAGATCC 
CGCAGCGTCA GTGGAGCATC CACTGGTCTG TCCTCCAGCC CTCTAAGCAG CCCAAGGAGT 
CCGGTCTTTT CCTTCTCACC GGAGCCAGGC GTTGGAGATG AGGCTAGAGG TGGAGGCTCC 
CCAACTTCCA AAACACAGAC GCTGCCTTCT CGGGGCCCCA GAGGTGGGGG CGCTGGAGAG 
CAGCCACCAC CTCCCAGTGC CCGTTCCACG CCCTTGCCTG GACCACCAGG CTCCCCGCGC 
TCCTCCGGCG GAACCCCCTT GCACTCACCT CTGCACACGC CTCGAGCCAG CCCTACCGGG 
ACTCCAGGAA CTACACCACC TCCCAGCCCA GGCGGTGGCG TCGGGGGAGC CGCCTGGAGA 
AGTCGTCTCA ACTCCATCCG CAACAGCTTC CTGGGCTCCC CTCGCTTCCA CCGGCGCAAG 
ATGCAGGTCC CTACCGCTGA GGAGATGTCC AGTTTGACAC CAGAATCCTC TCCAGAGCTG 
GCAAAACGCT CCTGGTTCGG GAATTTCATC TCCTTGGACA AAGAAGAACA AATATTCCTC 
GTGCTAAAGG ACAAACCTCT CAGCAGCATC AAAGCGGACA TTGTCCATGC CTTTCTGTCG 
ATCCCTAGCC TGAGTCACAG TGTGCTGTCA CAGACCAGCT TCAGGGCCGA ATACAAGGCC 
AGCGGTGGCC CCTCTGTCTT CCAGAAGCCT GTCCGCTTCC AGGTGGACAT CAGCTCCTCT 
GAGGGTCCAG AACCCTCACC CCGAAGAGAT GGTAGCAGTG GAGGTGGCAT CTACTCAGTC 
ACCTTCACTC TCATCTCCGG TCCTAGCCGT CGGTTCAAGC GTGTGGTAGA GACCATCCAG 
GCACAGTTGC TGAGTACTCA TGACCAGCCC TCCGTGCAGG CCTTGGCAGA TGAGAAGAAT 
GGAGCCCAGA CCCGGCCTGC TGGTACCCCA CCCCGAAGCC TGCAGCCTCC ACCAGGCCGC 
CCTGACCCTG ATCTGAGTAG CTCTCCCCGC CGAGGACCCT CTAAGGACAA GAAGCTCCTG 
GCCACCAATG GAACCCCTCT ACCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006228345.1
CDS	137..2203
Translation	MCSSSTTSTRTRNICRYLVLEHVSGGELFDYLVKKGRLTPKEARKFFRQIVSALDFCHSYSICHRDLKPENLLLDEKNNIRIADFGMASLQVGDSLLETSCGSPHYACPEVIKGEKYDGRRADMWSCGVILFALLVGALPFDDDNLRQLLEKVKRGVFHMPHFIPPDCQSLLRGMIEVEPEKRLSLEQIQKHPWYLGGKHEPDPCLEPAPGRRVAMRSLPSNGELDPDVLESMASLGCFRDRERLHRELRSEEENQEKMIYYLLLDRKERYPSCEDQDLPPRNDVDPPRKRVDSPMLSRHGKRRPERKSMEVLSITDAGSGGSPVPTRRALEMAQHSQRSRSVSGASTGLSSSPLSSPRSPVFSFSPEPGVGDEARGGGSPTSKTQTLPSRGPRGGGAGEQPPPPSARSTPLPGPPGSPRSSGGTPLHSPLHTPRASPTGTPGTTPPPSPGGGVGGAAWRSRLNSIRNSFLGSPRFHRRKMQVPTAEEMSSLTPESSPELAKRSWFGNFISLDKEEQIFLVLKDKPLSSIKADIVHAFLSIPSLSHSVLSQTSFRAEYKASGGPSVFQKPVRFQVDISSSEGPEPSPRRDGSSGGGIYSVTFTLISGPSRRFKRVVETIQAQLLSTHDQPSVQALADEKNGAQTRPAGTPPRSLQPPPGRPDPDLSSSPRRGPSKDKKLLATNGTPLP

Target ORF information:

RefSeq Version	XM_006228283.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus BR serine/threonine kinase 1 (Brsk1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006228283.2

ORF Insert Sequence:

ATGTGCTCAA GCTCCACGAC GTCTACGAGA ACAAGAAATA TTTGTAGATA CTTGGTGCTT 
GAGCATGTTT CTGGTGGTGA GCTGTTCGAC TACCTGGTAA AAAAAGGGAG ACTGACACCC 
AAGGAGGCCC GAAAGTTCTT CCGCCAGATT GTGTCAGCGC TGGACTTCTG CCATAGCTAC 
TCCATCTGTC ACAGGGACCT GAAGCCAGAG AACCTGCTGC TGGATGAGAA AAACAACATC 
CGCATCGCAG ACTTTGGCAT GGCGTCCCTG CAGGTGGGGG ACAGCCTCCT GGAGACCAGC 
TGTGGGTCCC CCCATTACGC ATGTCCAGAG GTGATCAAGG GGGAGAAGTA TGATGGCCGC 
CGGGCAGACA TGTGGAGCTG TGGAGTCATC CTGTTTGCCC TGCTTGTGGG GGCGCTGCCC 
TTCGATGATG ACAACCTACG ACAGCTACTA GAGAAGGTGA AACGTGGTGT CTTCCACATG 
CCGCACTTCA TCCCTCCAGA CTGCCAGAGC CTCCTGAGAG GGATGATTGA AGTGGAGCCC 
GAGAAAAGGC TCAGTCTGGA GCAAATTCAG AAACATCCTT GGTATCTGGG CGGGAAACAC 
GAACCAGACC CTTGCCTGGA GCCAGCCCCA GGCCGCAGAG TAGCTATGCG GAGCCTGCCT 
TCCAATGGCG AGCTGGACCC TGACGTTCTG GAAAGCATGG CGTCTCTGGG CTGCTTCAGA 
GACCGCGAGC GGCTACACAG AGAACTGCGA AGTGAGGAGG AAAACCAAGA AAAGATGATC 
TATTATTTGC TTTTGGATCG GAAAGAGCGG TACCCTAGCT GTGAAGACCA GGACCTGCCT 
CCTCGGAATG ATGTTGACCC ACCTCGGAAG CGTGTGGATT CCCCCATGCT GAGCAGACAC 
GGGAAACGGA GGCCAGAGCG GAAGTCCATG GAAGTCCTCA GCATCACAGA TGCTGGGAGT 
GGTGGCTCTC CAGTGCCTAC CAGAAGGGCC TTGGAGATGG CCCAGCACAG TCAGAGATCC 
CGCAGCGTCA GTGGAGCATC CACTGGTCTG TCCTCCAGCC CTCTAAGCAG CCCAAGGAGT 
CCGGTCTTTT CCTTCTCACC GGAGCCAGGC GTTGGAGATG AGGCTAGAGG TGGAGGCTCC 
CCAACTTCCA AAACACAGAC GCTGCCTTCT CGGGGCCCCA GAGGTGGGGG CGCTGGAGAG 
CAGCCACCAC CTCCCAGTGC CCGTTCCACG CCCTTGCCTG GACCACCAGG CTCCCCGCGC 
TCCTCCGGCG GAACCCCCTT GCACTCACCT CTGCACACGC CTCGAGCCAG CCCTACCGGG 
ACTCCAGGAA CTACACCACC TCCCAGCCCA GGCGGTGGCG TCGGGGGAGC CGCCTGGAGA 
AGTCGTCTCA ACTCCATCCG CAACAGCTTC CTGGGCTCCC CTCGCTTCCA CCGGCGCAAG 
ATGCAGGTCC CTACCGCTGA GGAGATGTCC AGTTTGACAC CAGAATCCTC TCCAGAGCTG 
GCAAAACGCT CCTGGTTCGG GAATTTCATC TCCTTGGACA AAGAAGAACA AATATTCCTC 
GTGCTAAAGG ACAAACCTCT CAGCAGCATC AAAGCGGACA TTGTCCATGC CTTTCTGTCG 
ATCCCTAGCC TGAGTCACAG TGTGCTGTCA CAGACCAGCT TCAGGGCCGA ATACAAGGCC 
AGCGGTGGCC CCTCTGTCTT CCAGAAGCCT GTCCGCTTCC AGGTGGACAT CAGCTCCTCT 
GAGGGTCCAG AACCCTCACC CCGAAGAGAT GGTAGCAGTG GAGGTGGCAT CTACTCAGTC 
ACCTTCACTC TCATCTCCGG TCCTAGCCGT CGGTTCAAGC GTGTGGTAGA GACCATCCAG 
GCACAGTTGC TGAGTACTCA TGACCAGCCC TCCGTGCAGG CCTTGGCAGA TGAGAAGAAT 
GGAGCCCAGA CCCGGCCTGC TGGTACCCCA CCCCGAAGCC TGCAGCCTCC ACCAGGCCGC 
CCTGACCCTG ATCTGAGTAG CTCTCCCCGC CGAGGACCCT CTAAGGACAA GAAGCTCCTG 
GCCACCAATG GAACCCCTCT ACCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

SAD-B kinase regulates pre-synaptic vesicular dynamics at hippocampal Schaffer collateral synapses and affects contextual fear memory.
Journal of neurochemistry136(1)36-47(2016 Jan)
Watabe AM,Nagase M,Hagiwara A,Hida Y,Tsuji M,Ochiai T,Kato F,Ohtsuka T

Polarized activities of AMPK and BRSK in primary hippocampal neurons.
Molecular biology of the cell26(10)1935-46(2015 May)
Sample V,Ramamurthy S,Gorshkov K,Ronnett GV,Zhang J

Phosphorylation of microtubule-associated protein tau by AMPK-related kinases.
Journal of neurochemistry120(1)165-76(2012 Jan)
Yoshida H,Goedert M

Persistence of the cell-cycle checkpoint kinase Wee1 in SadA- and SadB-deficient neurons disrupts neuronal polarity.
Journal of cell science123(Pt 2)286-94(2010 Jan)
M?ller M,Lutter D,P?schel AW

SADB phosphorylation of gamma-tubulin regulates centrosome duplication.
Nature cell biology11(9)1081-92(2009 Sep)
Alvarado-Kristensson M,Rodr?guez MJ,Sili? V,Valpuesta JM,Carrera AC

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Brsk1 cDNA ORF clone, Rattus norvegicus(Norway rat)

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Target ORF information:

ORF Insert Sequence:

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ORF Insert Sequence:

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