BRSK1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	BRSK1
Entrez Gene ID	84446
Full Name	BR serine/threonine kinase 1
Synonyms	hSAD1
General protein information	Preferred Names BR serine/threonine kinase 1 Names serine/threonine-protein kinase BRSK1 BR serine/threonine-protein kinase 1 SAD1 homolog SAD1 kinase SadB kinase short isoform brain-selective kinase 1 brain-specific serine/threonine-protein kinase 1 protein kinase SAD1A serine/threonine-protein kinase SAD-B synapses of Amphids Defective homolog 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19q13.42
Disorder MIM:	609235

mRNA and Protein(s)

mRNA	Protein	Name
XM_024451739.1	XP_024307507.1	serine/threonine-protein kinase BRSK1 isoform X1
XM_011527395.2	XP_011525697.2	serine/threonine-protein kinase BRSK1 isoform X3
XM_005259327.3	XP_005259384.1	serine/threonine-protein kinase BRSK1 isoform X2
NM_032430.2	NP_115806.1	serine/threonine-protein kinase BRSK1
NM_032430.1	NP_115806.1	serine/threonine-protein kinase BRSK1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	BRSK1	XP_541413.3
Rattus norvegicus (Norway rat)	Brsk1	NP_001120809.1
Danio rerio (zebrafish)	LOC558390	XP_003198179.2
Bos taurus (cattle)	BRSK1	XP_002695456.3
Xenopus tropicalis (tropical clawed frog)	brsk1	XP_004916365.1
Homo sapiens (human)	BRSK1	NP_115806.1
Mus musculus (house mouse)	Brsk1	NP_001003920.2
Danio rerio (zebrafish)	si:ch211-255p10.4	XP_693123.4

Gene Ontology
Bibliography

Related articles in PubMed

Roles of tau protein in health and disease.
Guo T, Noble W, Hanger DP
Acta neuropathologica133(5)665-704(2017 May)

SAD-B Phosphorylation of CAST Controls Active Zone Vesicle Recycling for Synaptic Depression.
Mochida S, Hida Y, Tanifuji S, Hagiwara A, Hamada S, Abe M, Ma H, Yasumura M, Kitajima I, Sakimura K, Ohtsuka T
Cell reports16(11)2901-2913(2016 09)

Putative Tumor Suppressor Genes EGR1 and BRSK1 Are Mutated in Gastric and Colorectal Cancers.
Choi EJ, Yoo NJ, Kim MS, An CH, Lee SH
Oncology91(5)289-294(2016)

SAD-B kinase regulates pre-synaptic vesicular dynamics at hippocampal Schaffer collateral synapses and affects contextual fear memory.
Watabe AM, Nagase M, Hagiwara A, Hida Y, Tsuji M, Ochiai T, Kato F, Ohtsuka T
Journal of neurochemistry136(1)36-47(2016 Jan)

Decreased expression and prognostic role of cytoplasmic BRSK1 in human breast carcinoma: correlation with Jab1 stability and PI3K/Akt pathway.
Wang H, Liu XB, Chen JH, Wang QQ, Chen JP, Xu JF, Sheng CY, Ni QC
Experimental and molecular pathology97(2)191-201(2014 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results suggest that frameshift mutations of EGR1 and BRSK1 might play a role in tumorigenesis through tumor suppressor gene inactivation in colorectal cancer and gastric cancer.
Title: Putative Tumor Suppressor Genes EGR1 and BRSK1 Are Mutated in Gastric and Colorectal Cancers.

BRSK1 is a novel tumor suppressor in breast cancer which inversely correlated with Jab1 expression.
Title: Decreased expression and prognostic role of cytoplasmic BRSK1 in human breast carcinoma: correlation with Jab1 stability and PI3K/Akt pathway.

Single nucleotide polymorphism in BRSK1 is associated with the length of reproductive lifespan.
Title: Replication of loci influencing ages at menarche and menopause in Hispanic women: the Women's Health Initiative SHARe Study.

SADB kinase activity controls centrosome homeostasis by regulating phosphorylation of gamma-tubulin.
Title: SADB phosphorylation of gamma-tubulin regulates centrosome duplication.

Observational study and genome-wide association study of gene-disease association. (HuGE Navigator)
Title: Genome-wide association studies identify loci associated with age at menarche and age at natural menopause.

The following BRSK1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BRSK1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu104768	XM_024451739.1 Latest version!	Homo sapiens BR serine/threonine kinase 1 (BRSK1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu30328	NM_032430.2 Latest version!	Homo sapiens BR serine/threonine kinase 1 (BRSK1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$545.30 $779.00
OHu52532	XM_005259327.3 Latest version!	Homo sapiens BR serine/threonine kinase 1 (BRSK1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OHu88762	XM_011527395.2 Latest version!	Homo sapiens BR serine/threonine kinase 1 (BRSK1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
View Old Accession Versions >>
OHu30328	NM_032430.1	Homo sapiens BR serine/threonine kinase 1 (BRSK1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$545.30 $779.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BRSK1 ( XM_024451739.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024307507.1 Homo sapiens BR serine/threonine kinase 1 (BRSK1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu104768
Clone ID Related Accession (Same CDS sequence)	XM_024451739.1
Accession Version	XM_024451739.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2112bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase BRSK1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTGG AGCGGGAGAT CGCCATCCTG AAGCTCATCG AACACCCACA TGTCCTCAAG 
CTCCACGACG TCTACGAGAA CAAGAAATAT TTGTACCTGG TTCTGGAGCA CGTCTCGGGG 
GGTGAGCTAT TCGACTACCT GGTAAAGAAG GGGAGACTGA CGCCCAAGGA GGCCCGAAAG 
TTCTTCCGCC AGATTGTGTC TGCGCTGGAC TTCTGCCACA GCTACTCCAT CTGCCACAGA 
GACCTAAAGC CCGAGAACCT GCTTTTGGAT GAGAAAAACA ACATCCGCAT TGCAGACTTC 
GGCATGGCGT CCCTGCAGGT GGGGGACAGC CTCCTGGAGA CCAGCTGCGG GTCCCCCCAT 
TATGCGTGTC CAGAGGTGAT TAAGGGGGAA AAATATGATG GCCGCCGGGC AGACATGTGG 
AGCTGTGGAG TCATCCTCTT CGCCCTGCTC GTGGGGGCTC TGCCCTTTGA TGACGACAAC 
CTCCGCCAGC TGCTGGAGAA GGTGAAACGG GGCGTCTTCC ACATGCCCCA CTTCATTCCT 
CCAGATTGCC AGAGCCTCCT GAGGGGAATG ATCGAAGTGG AGCCCGAAAA AAGGCTCAGT 
CTGGAGCAAA TTCAGAAACA TCCTTGGTAC CTAGGCGGGA AACACGAGCC AGACCCGTGC 
CTGGAGCCAG CCCCTGGCCG CCGGGTAGCC ATGCGGAGCC TGCCATCCAA CGGAGAGCTG 
GACCCCGACG TCCTAGAGAG CATGGCATCA CTGGGCTGCT TCAGGGACCG CGAGAGGCTG 
CATCGCGAGC TGCGCAGTGA GGAGGAGAAC CAAGAAAAGA TGATATATTA TCTGCTTTTG 
GATCGGAAGG AGCGGTATCC CAGCTGTGAG GACCAGGACC TGCCTCCCCG GAATGATGTT 
GACCCCCCCC GGAAGCGTGT GGATTCTCCC ATGCTGAGCC GTCACGGGAA GCGGCGACCA 
GAGCGGAAGT CCATGGAAGT CCTGAGCATC ACCGATGCCG GGGGTGGTGG CTCCCCTGTA 
CCCACCCGAC GGGCCTTGGA GATGGCCCAG CACAGCCAGA GATCCCGTAG CGTCAGTGGA 
GCCTCCACGG GTCTGTCCTC CAGCCCTCTA AGCAGCCCAA GGAGTCCGGT CTTTTCCTTT 
TCACCGGAGC CGGGGGCTGG AGATGAGGCT CGAGGCGGGG GCTCCCCGAC TTCCAAAACG 
CAGACGCTGC CTTCTCGGGG CCCCAGGGGT GGGGGCGCCG GGGAGCAGCC CCCGCCCCCC 
AGTGCCCGCT CCACACCCCT GCCCGGCCCC CCAGGCTCCC CGCGCTCCTC TGGCGGGACC 
CCCTTGCACT CGCCTCTGCA CACGCCCCGG GCCAGTCCCA CCGGGACCCC GGGGACAACA 
CCACCCCCCA GCCCCGGCGG TGGCGTCGGG GGAGCCGCCT GGAGGAGTCG TCTCAACTCC 
ATCCGCAACA GCTTCCTGGG CTCCCCTCGC TTTCACCGGC GCAAGATGCA GGTCCCTACC 
GCTGAGGAGA TGTCCAGCTT GACGCCAGAG TCCTCCCCGG AGCTGGCAAA ACGCTCCTGG 
TTCGGGAACT TCATCTCCTT GGACAAAGAA GAACAAATAT TCCTCGTGCT AAAGGACAAA 
CCTCTCAGCA GCATCAAAGC AGACATCGTC CATGCCTTTC TGTCGATCCC CAGCCTGAGT 
CACAGTGTGC TGTCACAGAC CAGCTTCAGG GCCGAGTACA AGGCCAGTGG CGGCCCCTCC 
GTCTTCCAAA AGCCCGTCCG CTTCCAGGTG GACATCAGCT CCTCTGAGGG TCCAGAGCCC 
TCCCCGCGAC GGGACGGCAG CGGAGGTGGT GGCATCTACT CCGTCACCTT CACTCTCATC 
TCGGGTCCCA GCCGTCGGTT CAAGCGAGTG GTGGAGACCA TCCAGGCACA GCTCCTGAGC 
ACTCATGACC AGCCCTCCGT GCAGGCCCTG GCAGACGAGA AGAACGGGGC CCAGACCCGG 
CCTGCTGGTG CCCCACCCCG AAGCCTGCAG CCCCCACCCG GCCGCCCAGA CCCAGAGCTG 
AGCAGCTCTC CCCGCCGAGG CCCCCCCAAG GACAAGAAGC TCCTGGCCAC CAACGGGACC 
CCTCTGCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024307507.1
CDS	177..2288
Translation	MKVEREIAILKLIEHPHVLKLHDVYENKKYLYLVLEHVSGGELFDYLVKKGRLTPKEARKFFRQIVSALDFCHSYSICHRDLKPENLLLDEKNNIRIADFGMASLQVGDSLLETSCGSPHYACPEVIKGEKYDGRRADMWSCGVILFALLVGALPFDDDNLRQLLEKVKRGVFHMPHFIPPDCQSLLRGMIEVEPEKRLSLEQIQKHPWYLGGKHEPDPCLEPAPGRRVAMRSLPSNGELDPDVLESMASLGCFRDRERLHRELRSEEENQEKMIYYLLLDRKERYPSCEDQDLPPRNDVDPPRKRVDSPMLSRHGKRRPERKSMEVLSITDAGGGGSPVPTRRALEMAQHSQRSRSVSGASTGLSSSPLSSPRSPVFSFSPEPGAGDEARGGGSPTSKTQTLPSRGPRGGGAGEQPPPPSARSTPLPGPPGSPRSSGGTPLHSPLHTPRASPTGTPGTTPPPSPGGGVGGAAWRSRLNSIRNSFLGSPRFHRRKMQVPTAEEMSSLTPESSPELAKRSWFGNFISLDKEEQIFLVLKDKPLSSIKADIVHAFLSIPSLSHSVLSQTSFRAEYKASGGPSVFQKPVRFQVDISSSEGPEPSPRRDGSGGGGIYSVTFTLISGPSRRFKRVVETIQAQLLSTHDQPSVQALADEKNGAQTRPAGAPPRSLQPPPGRPDPELSSSPRRGPPKDKKLLATNGTPLP

Target ORF information:

RefSeq Version	XM_024451739.1
Organism	Homo sapiens(human)
Definition	Homo sapiens BR serine/threonine kinase 1 (BRSK1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024451739.1

ORF Insert Sequence:

ATGAAGGTGG AGCGGGAGAT CGCCATCCTG AAGCTCATCG AACACCCACA TGTCCTCAAG 
CTCCACGACG TCTACGAGAA CAAGAAATAT TTGTACCTGG TTCTGGAGCA CGTCTCGGGG 
GGTGAGCTAT TCGACTACCT GGTAAAGAAG GGGAGACTGA CGCCCAAGGA GGCCCGAAAG 
TTCTTCCGCC AGATTGTGTC TGCGCTGGAC TTCTGCCACA GCTACTCCAT CTGCCACAGA 
GACCTAAAGC CCGAGAACCT GCTTTTGGAT GAGAAAAACA ACATCCGCAT TGCAGACTTC 
GGCATGGCGT CCCTGCAGGT GGGGGACAGC CTCCTGGAGA CCAGCTGCGG GTCCCCCCAT 
TATGCGTGTC CAGAGGTGAT TAAGGGGGAA AAATATGATG GCCGCCGGGC AGACATGTGG 
AGCTGTGGAG TCATCCTCTT CGCCCTGCTC GTGGGGGCTC TGCCCTTTGA TGACGACAAC 
CTCCGCCAGC TGCTGGAGAA GGTGAAACGG GGCGTCTTCC ACATGCCCCA CTTCATTCCT 
CCAGATTGCC AGAGCCTCCT GAGGGGAATG ATCGAAGTGG AGCCCGAAAA AAGGCTCAGT 
CTGGAGCAAA TTCAGAAACA TCCTTGGTAC CTAGGCGGGA AACACGAGCC AGACCCGTGC 
CTGGAGCCAG CCCCTGGCCG CCGGGTAGCC ATGCGGAGCC TGCCATCCAA CGGAGAGCTG 
GACCCCGACG TCCTAGAGAG CATGGCATCA CTGGGCTGCT TCAGGGACCG CGAGAGGCTG 
CATCGCGAGC TGCGCAGTGA GGAGGAGAAC CAAGAAAAGA TGATATATTA TCTGCTTTTG 
GATCGGAAGG AGCGGTATCC CAGCTGTGAG GACCAGGACC TGCCTCCCCG GAATGATGTT 
GACCCCCCCC GGAAGCGTGT GGATTCTCCC ATGCTGAGCC GTCACGGGAA GCGGCGACCA 
GAGCGGAAGT CCATGGAAGT CCTGAGCATC ACCGATGCCG GGGGTGGTGG CTCCCCTGTA 
CCCACCCGAC GGGCCTTGGA GATGGCCCAG CACAGCCAGA GATCCCGTAG CGTCAGTGGA 
GCCTCCACGG GTCTGTCCTC CAGCCCTCTA AGCAGCCCAA GGAGTCCGGT CTTTTCCTTT 
TCACCGGAGC CGGGGGCTGG AGATGAGGCT CGAGGCGGGG GCTCCCCGAC TTCCAAAACG 
CAGACGCTGC CTTCTCGGGG CCCCAGGGGT GGGGGCGCCG GGGAGCAGCC CCCGCCCCCC 
AGTGCCCGCT CCACACCCCT GCCCGGCCCC CCAGGCTCCC CGCGCTCCTC TGGCGGGACC 
CCCTTGCACT CGCCTCTGCA CACGCCCCGG GCCAGTCCCA CCGGGACCCC GGGGACAACA 
CCACCCCCCA GCCCCGGCGG TGGCGTCGGG GGAGCCGCCT GGAGGAGTCG TCTCAACTCC 
ATCCGCAACA GCTTCCTGGG CTCCCCTCGC TTTCACCGGC GCAAGATGCA GGTCCCTACC 
GCTGAGGAGA TGTCCAGCTT GACGCCAGAG TCCTCCCCGG AGCTGGCAAA ACGCTCCTGG 
TTCGGGAACT TCATCTCCTT GGACAAAGAA GAACAAATAT TCCTCGTGCT AAAGGACAAA 
CCTCTCAGCA GCATCAAAGC AGACATCGTC CATGCCTTTC TGTCGATCCC CAGCCTGAGT 
CACAGTGTGC TGTCACAGAC CAGCTTCAGG GCCGAGTACA AGGCCAGTGG CGGCCCCTCC 
GTCTTCCAAA AGCCCGTCCG CTTCCAGGTG GACATCAGCT CCTCTGAGGG TCCAGAGCCC 
TCCCCGCGAC GGGACGGCAG CGGAGGTGGT GGCATCTACT CCGTCACCTT CACTCTCATC 
TCGGGTCCCA GCCGTCGGTT CAAGCGAGTG GTGGAGACCA TCCAGGCACA GCTCCTGAGC 
ACTCATGACC AGCCCTCCGT GCAGGCCCTG GCAGACGAGA AGAACGGGGC CCAGACCCGG 
CCTGCTGGTG CCCCACCCCG AAGCCTGCAG CCCCCACCCG GCCGCCCAGA CCCAGAGCTG 
AGCAGCTCTC CCCGCCGAGG CCCCCCCAAG GACAAGAAGC TCCTGGCCAC CAACGGGACC 
CCTCTGCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BRSK1 ( NM_032430.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_115806.1 Homo sapiens BR serine/threonine kinase 1 (BRSK1), mRNA.

Price & Availability↑

CloneID	OHu30328
Clone ID Related Accession (Same CDS sequence)	NM_032430.2 , NM_032430.1
Accession Version	NM_032430.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2337bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase BRSK1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC008974.7 and AF479827.1. On May 17, 2019 this sequence version replaced NM_032430.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF479827.1, AL834275.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000309383.6/ ENSP00000310649.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END##

ATGTCGTCCG GGGCCAAGGA GGGAGGTGGG GGCTCTCCCG CCTACCACCT CCCCCACCCC 
CACCCCCACC CACCCCAGCA CGCCCAATAT GTGGGCCCCT ATCGGCTGGA GAAGACGCTG 
GGCAAAGGAC AGACAGGGCT GGTTAAACTC GGGGTCCACT GCATCACGGG TCAGAAGGTC 
GCCATCAAGA TCGTGAACCG GGAGAAGCTG TCGGAGTCGG TGCTGATGAA GGTGGAGCGG 
GAGATCGCCA TCCTGAAGCT CATCGAACAC CCACATGTCC TCAAGCTCCA CGACGTCTAC 
GAGAACAAGA AATATTTGTA CCTGGTTCTG GAGCACGTCT CGGGGGGTGA GCTATTCGAC 
TACCTGGTAA AGAAGGGGAG ACTGACGCCC AAGGAGGCCC GAAAGTTCTT CCGCCAGATT 
GTGTCTGCGC TGGACTTCTG CCACAGCTAC TCCATCTGCC ACAGAGACCT AAAGCCCGAG 
AACCTGCTTT TGGATGAGAA AAACAACATC CGCATTGCAG ACTTCGGCAT GGCGTCCCTG 
CAGGTGGGGG ACAGCCTCCT GGAGACCAGC TGCGGGTCCC CCCATTATGC GTGTCCAGAG 
GTGATTAAGG GGGAAAAATA TGATGGCCGC CGGGCAGACA TGTGGAGCTG TGGAGTCATC 
CTCTTCGCCC TGCTCGTGGG GGCTCTGCCC TTTGATGACG ACAACCTCCG CCAGCTGCTG 
GAGAAGGTGA AACGGGGCGT CTTCCACATG CCCCACTTCA TTCCTCCAGA TTGCCAGAGC 
CTCCTGAGGG GAATGATCGA AGTGGAGCCC GAAAAAAGGC TCAGTCTGGA GCAAATTCAG 
AAACATCCTT GGTACCTAGG CGGGAAACAC GAGCCAGACC CGTGCCTGGA GCCAGCCCCT 
GGCCGCCGGG TAGCCATGCG GAGCCTGCCA TCCAACGGAG AGCTGGACCC CGACGTCCTA 
GAGAGCATGG CATCACTGGG CTGCTTCAGG GACCGCGAGA GGCTGCATCG CGAGCTGCGC 
AGTGAGGAGG AGAACCAAGA AAAGATGATA TATTATCTGC TTTTGGATCG GAAGGAGCGG 
TATCCCAGCT GTGAGGACCA GGACCTGCCT CCCCGGAATG ATGTTGACCC CCCCCGGAAG 
CGTGTGGATT CTCCCATGCT GAGCCGTCAC GGGAAGCGGC GACCAGAGCG GAAGTCCATG 
GAAGTCCTGA GCATCACCGA TGCCGGGGGT GGTGGCTCCC CTGTACCCAC CCGACGGGCC 
TTGGAGATGG CCCAGCACAG CCAGAGATCC CGTAGCGTCA GTGGAGCCTC CACGGGTCTG 
TCCTCCAGCC CTCTAAGCAG CCCAAGGAGT CCGGTCTTTT CCTTTTCACC GGAGCCGGGG 
GCTGGAGATG AGGCTCGAGG CGGGGGCTCC CCGACTTCCA AAACGCAGAC GCTGCCTTCT 
CGGGGCCCCA GGGGTGGGGG CGCCGGGGAG CAGCCCCCGC CCCCCAGTGC CCGCTCCACA 
CCCCTGCCCG GCCCCCCAGG CTCCCCGCGC TCCTCTGGCG GGACCCCCTT GCACTCGCCT 
CTGCACACGC CCCGGGCCAG TCCCACCGGG ACCCCGGGGA CAACACCACC CCCCAGCCCC 
GGCGGTGGCG TCGGGGGAGC CGCCTGGAGG AGTCGTCTCA ACTCCATCCG CAACAGCTTC 
CTGGGCTCCC CTCGCTTTCA CCGGCGCAAG ATGCAGGTCC CTACCGCTGA GGAGATGTCC 
AGCTTGACGC CAGAGTCCTC CCCGGAGCTG GCAAAACGCT CCTGGTTCGG GAACTTCATC 
TCCTTGGACA AAGAAGAACA AATATTCCTC GTGCTAAAGG ACAAACCTCT CAGCAGCATC 
AAAGCAGACA TCGTCCATGC CTTTCTGTCG ATCCCCAGCC TGAGTCACAG TGTGCTGTCA 
CAGACCAGCT TCAGGGCCGA GTACAAGGCC AGTGGCGGCC CCTCCGTCTT CCAAAAGCCC 
GTCCGCTTCC AGGTGGACAT CAGCTCCTCT GAGGGTCCAG AGCCCTCCCC GCGACGGGAC 
GGCAGCGGAG GTGGTGGCAT CTACTCCGTC ACCTTCACTC TCATCTCGGG TCCCAGCCGT 
CGGTTCAAGC GAGTGGTGGA GACCATCCAG GCACAGCTCC TGAGCACTCA TGACCAGCCC 
TCCGTGCAGG CCCTGGCAGA CGAGAAGAAC GGGGCCCAGA CCCGGCCTGC TGGTGCCCCA 
CCCCGAAGCC TGCAGCCCCC ACCCGGCCGC CCAGACCCAG AGCTGAGCAG CTCTCCCCGC 
CGAGGCCCCC CCAAGGACAA GAAGCTCCTG GCCACCAACG GGACCCCTCT GCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_115806.1
CDS	447..2783
Translation	MSSGAKEGGGGSPAYHLPHPHPHPPQHAQYVGPYRLEKTLGKGQTGLVKLGVHCITGQKVAIKIVNREKLSESVLMKVEREIAILKLIEHPHVLKLHDVYENKKYLYLVLEHVSGGELFDYLVKKGRLTPKEARKFFRQIVSALDFCHSYSICHRDLKPENLLLDEKNNIRIADFGMASLQVGDSLLETSCGSPHYACPEVIKGEKYDGRRADMWSCGVILFALLVGALPFDDDNLRQLLEKVKRGVFHMPHFIPPDCQSLLRGMIEVEPEKRLSLEQIQKHPWYLGGKHEPDPCLEPAPGRRVAMRSLPSNGELDPDVLESMASLGCFRDRERLHRELRSEEENQEKMIYYLLLDRKERYPSCEDQDLPPRNDVDPPRKRVDSPMLSRHGKRRPERKSMEVLSITDAGGGGSPVPTRRALEMAQHSQRSRSVSGASTGLSSSPLSSPRSPVFSFSPEPGAGDEARGGGSPTSKTQTLPSRGPRGGGAGEQPPPPSARSTPLPGPPGSPRSSGGTPLHSPLHTPRASPTGTPGTTPPPSPGGGVGGAAWRSRLNSIRNSFLGSPRFHRRKMQVPTAEEMSSLTPESSPELAKRSWFGNFISLDKEEQIFLVLKDKPLSSIKADIVHAFLSIPSLSHSVLSQTSFRAEYKASGGPSVFQKPVRFQVDISSSEGPEPSPRRDGSGGGGIYSVTFTLISGPSRRFKRVVETIQAQLLSTHDQPSVQALADEKNGAQTRPAGAPPRSLQPPPGRPDPELSSSPRRGPPKDKKLLATNGTPLP

Target ORF information:

RefSeq Version	NM_032430.2
Organism	Homo sapiens(human)
Definition	Homo sapiens BR serine/threonine kinase 1 (BRSK1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032430.2

ORF Insert Sequence:

ATGTCGTCCG GGGCCAAGGA GGGAGGTGGG GGCTCTCCCG CCTACCACCT CCCCCACCCC 
CACCCCCACC CACCCCAGCA CGCCCAATAT GTGGGCCCCT ATCGGCTGGA GAAGACGCTG 
GGCAAAGGAC AGACAGGGCT GGTTAAACTC GGGGTCCACT GCATCACGGG TCAGAAGGTC 
GCCATCAAGA TCGTGAACCG GGAGAAGCTG TCGGAGTCGG TGCTGATGAA GGTGGAGCGG 
GAGATCGCCA TCCTGAAGCT CATCGAACAC CCACATGTCC TCAAGCTCCA CGACGTCTAC 
GAGAACAAGA AATATTTGTA CCTGGTTCTG GAGCACGTCT CGGGGGGTGA GCTATTCGAC 
TACCTGGTAA AGAAGGGGAG ACTGACGCCC AAGGAGGCCC GAAAGTTCTT CCGCCAGATT 
GTGTCTGCGC TGGACTTCTG CCACAGCTAC TCCATCTGCC ACAGAGACCT AAAGCCCGAG 
AACCTGCTTT TGGATGAGAA AAACAACATC CGCATTGCAG ACTTCGGCAT GGCGTCCCTG 
CAGGTGGGGG ACAGCCTCCT GGAGACCAGC TGCGGGTCCC CCCATTATGC GTGTCCAGAG 
GTGATTAAGG GGGAAAAATA TGATGGCCGC CGGGCAGACA TGTGGAGCTG TGGAGTCATC 
CTCTTCGCCC TGCTCGTGGG GGCTCTGCCC TTTGATGACG ACAACCTCCG CCAGCTGCTG 
GAGAAGGTGA AACGGGGCGT CTTCCACATG CCCCACTTCA TTCCTCCAGA TTGCCAGAGC 
CTCCTGAGGG GAATGATCGA AGTGGAGCCC GAAAAAAGGC TCAGTCTGGA GCAAATTCAG 
AAACATCCTT GGTACCTAGG CGGGAAACAC GAGCCAGACC CGTGCCTGGA GCCAGCCCCT 
GGCCGCCGGG TAGCCATGCG GAGCCTGCCA TCCAACGGAG AGCTGGACCC CGACGTCCTA 
GAGAGCATGG CATCACTGGG CTGCTTCAGG GACCGCGAGA GGCTGCATCG CGAGCTGCGC 
AGTGAGGAGG AGAACCAAGA AAAGATGATA TATTATCTGC TTTTGGATCG GAAGGAGCGG 
TATCCCAGCT GTGAGGACCA GGACCTGCCT CCCCGGAATG ATGTTGACCC CCCCCGGAAG 
CGTGTGGATT CTCCCATGCT GAGCCGTCAC GGGAAGCGGC GACCAGAGCG GAAGTCCATG 
GAAGTCCTGA GCATCACCGA TGCCGGGGGT GGTGGCTCCC CTGTACCCAC CCGACGGGCC 
TTGGAGATGG CCCAGCACAG CCAGAGATCC CGTAGCGTCA GTGGAGCCTC CACGGGTCTG 
TCCTCCAGCC CTCTAAGCAG CCCAAGGAGT CCGGTCTTTT CCTTTTCACC GGAGCCGGGG 
GCTGGAGATG AGGCTCGAGG CGGGGGCTCC CCGACTTCCA AAACGCAGAC GCTGCCTTCT 
CGGGGCCCCA GGGGTGGGGG CGCCGGGGAG CAGCCCCCGC CCCCCAGTGC CCGCTCCACA 
CCCCTGCCCG GCCCCCCAGG CTCCCCGCGC TCCTCTGGCG GGACCCCCTT GCACTCGCCT 
CTGCACACGC CCCGGGCCAG TCCCACCGGG ACCCCGGGGA CAACACCACC CCCCAGCCCC 
GGCGGTGGCG TCGGGGGAGC CGCCTGGAGG AGTCGTCTCA ACTCCATCCG CAACAGCTTC 
CTGGGCTCCC CTCGCTTTCA CCGGCGCAAG ATGCAGGTCC CTACCGCTGA GGAGATGTCC 
AGCTTGACGC CAGAGTCCTC CCCGGAGCTG GCAAAACGCT CCTGGTTCGG GAACTTCATC 
TCCTTGGACA AAGAAGAACA AATATTCCTC GTGCTAAAGG ACAAACCTCT CAGCAGCATC 
AAAGCAGACA TCGTCCATGC CTTTCTGTCG ATCCCCAGCC TGAGTCACAG TGTGCTGTCA 
CAGACCAGCT TCAGGGCCGA GTACAAGGCC AGTGGCGGCC CCTCCGTCTT CCAAAAGCCC 
GTCCGCTTCC AGGTGGACAT CAGCTCCTCT GAGGGTCCAG AGCCCTCCCC GCGACGGGAC 
GGCAGCGGAG GTGGTGGCAT CTACTCCGTC ACCTTCACTC TCATCTCGGG TCCCAGCCGT 
CGGTTCAAGC GAGTGGTGGA GACCATCCAG GCACAGCTCC TGAGCACTCA TGACCAGCCC 
TCCGTGCAGG CCCTGGCAGA CGAGAAGAAC GGGGCCCAGA CCCGGCCTGC TGGTGCCCCA 
CCCCGAAGCC TGCAGCCCCC ACCCGGCCGC CCAGACCCAG AGCTGAGCAG CTCTCCCCGC 
CGAGGCCCCC CCAAGGACAA GAAGCTCCTG GCCACCAACG GGACCCCTCT GCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BRSK1 ( NM_032430.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_115806.1 Homo sapiens BR serine/threonine kinase 1 (BRSK1), mRNA.

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CloneID	OHu30328
Clone ID Related Accession (Same CDS sequence)	NM_032430.2 , NM_032430.1
Accession Version	NM_032430.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2337bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-15
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase BRSK1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF479827.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF479827.1, AY458602.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END##

ATGTCGTCCG GGGCCAAGGA GGGAGGTGGG GGCTCTCCCG CCTACCACCT CCCCCACCCC 
CACCCCCACC CACCCCAGCA CGCCCAATAT GTGGGCCCCT ATCGGCTGGA GAAGACGCTG 
GGCAAAGGAC AGACAGGGCT GGTTAAACTC GGGGTCCACT GCATCACGGG TCAGAAGGTC 
GCCATCAAGA TCGTGAACCG GGAGAAGCTG TCGGAGTCGG TGCTGATGAA GGTGGAGCGG 
GAGATCGCCA TCCTGAAGCT CATCGAACAC CCACATGTCC TCAAGCTCCA CGACGTCTAC 
GAGAACAAGA AATATTTGTA CCTGGTTCTG GAGCACGTCT CGGGGGGTGA GCTATTCGAC 
TACCTGGTAA AGAAGGGGAG ACTGACGCCC AAGGAGGCCC GAAAGTTCTT CCGCCAGATT 
GTGTCTGCGC TGGACTTCTG CCACAGCTAC TCCATCTGCC ACAGAGACCT AAAGCCCGAG 
AACCTGCTTT TGGATGAGAA AAACAACATC CGCATTGCAG ACTTCGGCAT GGCGTCCCTG 
CAGGTGGGGG ACAGCCTCCT GGAGACCAGC TGCGGGTCCC CCCATTATGC GTGTCCAGAG 
GTGATTAAGG GGGAAAAATA TGATGGCCGC CGGGCAGACA TGTGGAGCTG TGGAGTCATC 
CTCTTCGCCC TGCTCGTGGG GGCTCTGCCC TTTGATGACG ACAACCTCCG CCAGCTGCTG 
GAGAAGGTGA AACGGGGCGT CTTCCACATG CCCCACTTCA TTCCTCCAGA TTGCCAGAGC 
CTCCTGAGGG GAATGATCGA AGTGGAGCCC GAAAAAAGGC TCAGTCTGGA GCAAATTCAG 
AAACATCCTT GGTACCTAGG CGGGAAACAC GAGCCAGACC CGTGCCTGGA GCCAGCCCCT 
GGCCGCCGGG TAGCCATGCG GAGCCTGCCA TCCAACGGAG AGCTGGACCC CGACGTCCTA 
GAGAGCATGG CATCACTGGG CTGCTTCAGG GACCGCGAGA GGCTGCATCG CGAGCTGCGC 
AGTGAGGAGG AGAACCAAGA AAAGATGATA TATTATCTGC TTTTGGATCG GAAGGAGCGG 
TATCCCAGCT GTGAGGACCA GGACCTGCCT CCCCGGAATG ATGTTGACCC CCCCCGGAAG 
CGTGTGGATT CTCCCATGCT GAGCCGTCAC GGGAAGCGGC GACCAGAGCG GAAGTCCATG 
GAAGTCCTGA GCATCACCGA TGCCGGGGGT GGTGGCTCCC CTGTACCCAC CCGACGGGCC 
TTGGAGATGG CCCAGCACAG CCAGAGATCC CGTAGCGTCA GTGGAGCCTC CACGGGTCTG 
TCCTCCAGCC CTCTAAGCAG CCCAAGGAGT CCGGTCTTTT CCTTTTCACC GGAGCCGGGG 
GCTGGAGATG AGGCTCGAGG CGGGGGCTCC CCGACTTCCA AAACGCAGAC GCTGCCTTCT 
CGGGGCCCCA GGGGTGGGGG CGCCGGGGAG CAGCCCCCGC CCCCCAGTGC CCGCTCCACA 
CCCCTGCCCG GCCCCCCAGG CTCCCCGCGC TCCTCTGGCG GGACCCCCTT GCACTCGCCT 
CTGCACACGC CCCGGGCCAG TCCCACCGGG ACCCCGGGGA CAACACCACC CCCCAGCCCC 
GGCGGTGGCG TCGGGGGAGC CGCCTGGAGG AGTCGTCTCA ACTCCATCCG CAACAGCTTC 
CTGGGCTCCC CTCGCTTTCA CCGGCGCAAG ATGCAGGTCC CTACCGCTGA GGAGATGTCC 
AGCTTGACGC CAGAGTCCTC CCCGGAGCTG GCAAAACGCT CCTGGTTCGG GAACTTCATC 
TCCTTGGACA AAGAAGAACA AATATTCCTC GTGCTAAAGG ACAAACCTCT CAGCAGCATC 
AAAGCAGACA TCGTCCATGC CTTTCTGTCG ATCCCCAGCC TGAGTCACAG TGTGCTGTCA 
CAGACCAGCT TCAGGGCCGA GTACAAGGCC AGTGGCGGCC CCTCCGTCTT CCAAAAGCCC 
GTCCGCTTCC AGGTGGACAT CAGCTCCTCT GAGGGTCCAG AGCCCTCCCC GCGACGGGAC 
GGCAGCGGAG GTGGTGGCAT CTACTCCGTC ACCTTCACTC TCATCTCGGG TCCCAGCCGT 
CGGTTCAAGC GAGTGGTGGA GACCATCCAG GCACAGCTCC TGAGCACTCA TGACCAGCCC 
TCCGTGCAGG CCCTGGCAGA CGAGAAGAAC GGGGCCCAGA CCCGGCCTGC TGGTGCCCCA 
CCCCGAAGCC TGCAGCCCCC ACCCGGCCGC CCAGACCCAG AGCTGAGCAG CTCTCCCCGC 
CGAGGCCCCC CCAAGGACAA GAAGCTCCTG GCCACCAACG GGACCCCTCT GCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_115806.1
CDS	278..2614
Translation	MSSGAKEGGGGSPAYHLPHPHPHPPQHAQYVGPYRLEKTLGKGQTGLVKLGVHCITGQKVAIKIVNREKLSESVLMKVEREIAILKLIEHPHVLKLHDVYENKKYLYLVLEHVSGGELFDYLVKKGRLTPKEARKFFRQIVSALDFCHSYSICHRDLKPENLLLDEKNNIRIADFGMASLQVGDSLLETSCGSPHYACPEVIKGEKYDGRRADMWSCGVILFALLVGALPFDDDNLRQLLEKVKRGVFHMPHFIPPDCQSLLRGMIEVEPEKRLSLEQIQKHPWYLGGKHEPDPCLEPAPGRRVAMRSLPSNGELDPDVLESMASLGCFRDRERLHRELRSEEENQEKMIYYLLLDRKERYPSCEDQDLPPRNDVDPPRKRVDSPMLSRHGKRRPERKSMEVLSITDAGGGGSPVPTRRALEMAQHSQRSRSVSGASTGLSSSPLSSPRSPVFSFSPEPGAGDEARGGGSPTSKTQTLPSRGPRGGGAGEQPPPPSARSTPLPGPPGSPRSSGGTPLHSPLHTPRASPTGTPGTTPPPSPGGGVGGAAWRSRLNSIRNSFLGSPRFHRRKMQVPTAEEMSSLTPESSPELAKRSWFGNFISLDKEEQIFLVLKDKPLSSIKADIVHAFLSIPSLSHSVLSQTSFRAEYKASGGPSVFQKPVRFQVDISSSEGPEPSPRRDGSGGGGIYSVTFTLISGPSRRFKRVVETIQAQLLSTHDQPSVQALADEKNGAQTRPAGAPPRSLQPPPGRPDPELSSSPRRGPPKDKKLLATNGTPLP

Target ORF information:

RefSeq Version	NM_032430.1
Organism	Homo sapiens(human)
Definition	Homo sapiens BR serine/threonine kinase 1 (BRSK1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032430.1

ORF Insert Sequence:

ATGTCGTCCG GGGCCAAGGA GGGAGGTGGG GGCTCTCCCG CCTACCACCT CCCCCACCCC 
CACCCCCACC CACCCCAGCA CGCCCAATAT GTGGGCCCCT ATCGGCTGGA GAAGACGCTG 
GGCAAAGGAC AGACAGGGCT GGTTAAACTC GGGGTCCACT GCATCACGGG TCAGAAGGTC 
GCCATCAAGA TCGTGAACCG GGAGAAGCTG TCGGAGTCGG TGCTGATGAA GGTGGAGCGG 
GAGATCGCCA TCCTGAAGCT CATCGAACAC CCACATGTCC TCAAGCTCCA CGACGTCTAC 
GAGAACAAGA AATATTTGTA CCTGGTTCTG GAGCACGTCT CGGGGGGTGA GCTATTCGAC 
TACCTGGTAA AGAAGGGGAG ACTGACGCCC AAGGAGGCCC GAAAGTTCTT CCGCCAGATT 
GTGTCTGCGC TGGACTTCTG CCACAGCTAC TCCATCTGCC ACAGAGACCT AAAGCCCGAG 
AACCTGCTTT TGGATGAGAA AAACAACATC CGCATTGCAG ACTTCGGCAT GGCGTCCCTG 
CAGGTGGGGG ACAGCCTCCT GGAGACCAGC TGCGGGTCCC CCCATTATGC GTGTCCAGAG 
GTGATTAAGG GGGAAAAATA TGATGGCCGC CGGGCAGACA TGTGGAGCTG TGGAGTCATC 
CTCTTCGCCC TGCTCGTGGG GGCTCTGCCC TTTGATGACG ACAACCTCCG CCAGCTGCTG 
GAGAAGGTGA AACGGGGCGT CTTCCACATG CCCCACTTCA TTCCTCCAGA TTGCCAGAGC 
CTCCTGAGGG GAATGATCGA AGTGGAGCCC GAAAAAAGGC TCAGTCTGGA GCAAATTCAG 
AAACATCCTT GGTACCTAGG CGGGAAACAC GAGCCAGACC CGTGCCTGGA GCCAGCCCCT 
GGCCGCCGGG TAGCCATGCG GAGCCTGCCA TCCAACGGAG AGCTGGACCC CGACGTCCTA 
GAGAGCATGG CATCACTGGG CTGCTTCAGG GACCGCGAGA GGCTGCATCG CGAGCTGCGC 
AGTGAGGAGG AGAACCAAGA AAAGATGATA TATTATCTGC TTTTGGATCG GAAGGAGCGG 
TATCCCAGCT GTGAGGACCA GGACCTGCCT CCCCGGAATG ATGTTGACCC CCCCCGGAAG 
CGTGTGGATT CTCCCATGCT GAGCCGTCAC GGGAAGCGGC GACCAGAGCG GAAGTCCATG 
GAAGTCCTGA GCATCACCGA TGCCGGGGGT GGTGGCTCCC CTGTACCCAC CCGACGGGCC 
TTGGAGATGG CCCAGCACAG CCAGAGATCC CGTAGCGTCA GTGGAGCCTC CACGGGTCTG 
TCCTCCAGCC CTCTAAGCAG CCCAAGGAGT CCGGTCTTTT CCTTTTCACC GGAGCCGGGG 
GCTGGAGATG AGGCTCGAGG CGGGGGCTCC CCGACTTCCA AAACGCAGAC GCTGCCTTCT 
CGGGGCCCCA GGGGTGGGGG CGCCGGGGAG CAGCCCCCGC CCCCCAGTGC CCGCTCCACA 
CCCCTGCCCG GCCCCCCAGG CTCCCCGCGC TCCTCTGGCG GGACCCCCTT GCACTCGCCT 
CTGCACACGC CCCGGGCCAG TCCCACCGGG ACCCCGGGGA CAACACCACC CCCCAGCCCC 
GGCGGTGGCG TCGGGGGAGC CGCCTGGAGG AGTCGTCTCA ACTCCATCCG CAACAGCTTC 
CTGGGCTCCC CTCGCTTTCA CCGGCGCAAG ATGCAGGTCC CTACCGCTGA GGAGATGTCC 
AGCTTGACGC CAGAGTCCTC CCCGGAGCTG GCAAAACGCT CCTGGTTCGG GAACTTCATC 
TCCTTGGACA AAGAAGAACA AATATTCCTC GTGCTAAAGG ACAAACCTCT CAGCAGCATC 
AAAGCAGACA TCGTCCATGC CTTTCTGTCG ATCCCCAGCC TGAGTCACAG TGTGCTGTCA 
CAGACCAGCT TCAGGGCCGA GTACAAGGCC AGTGGCGGCC CCTCCGTCTT CCAAAAGCCC 
GTCCGCTTCC AGGTGGACAT CAGCTCCTCT GAGGGTCCAG AGCCCTCCCC GCGACGGGAC 
GGCAGCGGAG GTGGTGGCAT CTACTCCGTC ACCTTCACTC TCATCTCGGG TCCCAGCCGT 
CGGTTCAAGC GAGTGGTGGA GACCATCCAG GCACAGCTCC TGAGCACTCA TGACCAGCCC 
TCCGTGCAGG CCCTGGCAGA CGAGAAGAAC GGGGCCCAGA CCCGGCCTGC TGGTGCCCCA 
CCCCGAAGCC TGCAGCCCCC ACCCGGCCGC CCAGACCCAG AGCTGAGCAG CTCTCCCCGC 
CGAGGCCCCC CCAAGGACAA GAAGCTCCTG GCCACCAACG GGACCCCTCT GCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BRSK1 ( XM_005259327.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005259384.1 Homo sapiens BR serine/threonine kinase 1 (BRSK1), transcript variant X2, mRNA.

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CloneID	OHu52532
Clone ID Related Accession (Same CDS sequence)	XM_005259327.3
Accession Version	XM_005259327.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2067bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase BRSK1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005259327.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTCAA GCTCCACGAC GTCTACGAGA ACAAGAAATA TTTGTAGGTA CCTGGTTCTG 
GAGCACGTCT CGGGGGGTGA GCTATTCGAC TACCTGGTAA AGAAGGGGAG ACTGACGCCC 
AAGGAGGCCC GAAAGTTCTT CCGCCAGATT GTGTCTGCGC TGGACTTCTG CCACAGCTAC 
TCCATCTGCC ACAGAGACCT AAAGCCCGAG AACCTGCTTT TGGATGAGAA AAACAACATC 
CGCATTGCAG ACTTCGGCAT GGCGTCCCTG CAGGTGGGGG ACAGCCTCCT GGAGACCAGC 
TGCGGGTCCC CCCATTATGC GTGTCCAGAG GTGATTAAGG GGGAAAAATA TGATGGCCGC 
CGGGCAGACA TGTGGAGCTG TGGAGTCATC CTCTTCGCCC TGCTCGTGGG GGCTCTGCCC 
TTTGATGACG ACAACCTCCG CCAGCTGCTG GAGAAGGTGA AACGGGGCGT CTTCCACATG 
CCCCACTTCA TTCCTCCAGA TTGCCAGAGC CTCCTGAGGG GAATGATCGA AGTGGAGCCC 
GAAAAAAGGC TCAGTCTGGA GCAAATTCAG AAACATCCTT GGTACCTAGG CGGGAAACAC 
GAGCCAGACC CGTGCCTGGA GCCAGCCCCT GGCCGCCGGG TAGCCATGCG GAGCCTGCCA 
TCCAACGGAG AGCTGGACCC CGACGTCCTA GAGAGCATGG CATCACTGGG CTGCTTCAGG 
GACCGCGAGA GGCTGCATCG CGAGCTGCGC AGTGAGGAGG AGAACCAAGA AAAGATGATA 
TATTATCTGC TTTTGGATCG GAAGGAGCGG TATCCCAGCT GTGAGGACCA GGACCTGCCT 
CCCCGGAATG ATGTTGACCC CCCCCGGAAG CGTGTGGATT CTCCCATGCT GAGCCGTCAC 
GGGAAGCGGC GACCAGAGCG GAAGTCCATG GAAGTCCTGA GCATCACCGA TGCCGGGGGT 
GGTGGCTCCC CTGTACCCAC CCGACGGGCC TTGGAGATGG CCCAGCACAG CCAGAGATCC 
CGTAGCGTCA GTGGAGCCTC CACGGGTCTG TCCTCCAGCC CTCTAAGCAG CCCAAGGAGT 
CCGGTCTTTT CCTTTTCACC GGAGCCGGGG GCTGGAGATG AGGCTCGAGG CGGGGGCTCC 
CCGACTTCCA AAACGCAGAC GCTGCCTTCT CGGGGCCCCA GGGGTGGGGG CGCCGGGGAG 
CAGCCCCCGC CCCCCAGTGC CCGCTCCACA CCCCTGCCCG GCCCCCCAGG CTCCCCGCGC 
TCCTCTGGCG GGACCCCCTT GCACTCGCCT CTGCACACGC CCCGGGCCAG TCCCACCGGG 
ACCCCGGGGA CAACACCACC CCCCAGCCCC GGCGGTGGCG TCGGGGGAGC CGCCTGGAGG 
AGTCGTCTCA ACTCCATCCG CAACAGCTTC CTGGGCTCCC CTCGCTTTCA CCGGCGCAAG 
ATGCAGGTCC CTACCGCTGA GGAGATGTCC AGCTTGACGC CAGAGTCCTC CCCGGAGCTG 
GCAAAACGCT CCTGGTTCGG GAACTTCATC TCCTTGGACA AAGAAGAACA AATATTCCTC 
GTGCTAAAGG ACAAACCTCT CAGCAGCATC AAAGCAGACA TCGTCCATGC CTTTCTGTCG 
ATCCCCAGCC TGAGTCACAG TGTGCTGTCA CAGACCAGCT TCAGGGCCGA GTACAAGGCC 
AGTGGCGGCC CCTCCGTCTT CCAAAAGCCC GTCCGCTTCC AGGTGGACAT CAGCTCCTCT 
GAGGGTCCAG AGCCCTCCCC GCGACGGGAC GGCAGCGGAG GTGGTGGCAT CTACTCCGTC 
ACCTTCACTC TCATCTCGGG TCCCAGCCGT CGGTTCAAGC GAGTGGTGGA GACCATCCAG 
GCACAGCTCC TGAGCACTCA TGACCAGCCC TCCGTGCAGG CCCTGGCAGA CGAGAAGAAC 
GGGGCCCAGA CCCGGCCTGC TGGTGCCCCA CCCCGAAGCC TGCAGCCCCC ACCCGGCCGC 
CCAGACCCAG AGCTGAGCAG CTCTCCCCGC CGAGGCCCCC CCAAGGACAA GAAGCTCCTG 
GCCACCAACG GGACCCCTCT GCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005259384.1
CDS	617..2683
Translation	MSSSSTTSTRTRNICRYLVLEHVSGGELFDYLVKKGRLTPKEARKFFRQIVSALDFCHSYSICHRDLKPENLLLDEKNNIRIADFGMASLQVGDSLLETSCGSPHYACPEVIKGEKYDGRRADMWSCGVILFALLVGALPFDDDNLRQLLEKVKRGVFHMPHFIPPDCQSLLRGMIEVEPEKRLSLEQIQKHPWYLGGKHEPDPCLEPAPGRRVAMRSLPSNGELDPDVLESMASLGCFRDRERLHRELRSEEENQEKMIYYLLLDRKERYPSCEDQDLPPRNDVDPPRKRVDSPMLSRHGKRRPERKSMEVLSITDAGGGGSPVPTRRALEMAQHSQRSRSVSGASTGLSSSPLSSPRSPVFSFSPEPGAGDEARGGGSPTSKTQTLPSRGPRGGGAGEQPPPPSARSTPLPGPPGSPRSSGGTPLHSPLHTPRASPTGTPGTTPPPSPGGGVGGAAWRSRLNSIRNSFLGSPRFHRRKMQVPTAEEMSSLTPESSPELAKRSWFGNFISLDKEEQIFLVLKDKPLSSIKADIVHAFLSIPSLSHSVLSQTSFRAEYKASGGPSVFQKPVRFQVDISSSEGPEPSPRRDGSGGGGIYSVTFTLISGPSRRFKRVVETIQAQLLSTHDQPSVQALADEKNGAQTRPAGAPPRSLQPPPGRPDPELSSSPRRGPPKDKKLLATNGTPLP

Target ORF information:

RefSeq Version	XM_005259327.3
Organism	Homo sapiens(human)
Definition	Homo sapiens BR serine/threonine kinase 1 (BRSK1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005259327.3

ORF Insert Sequence:

ATGTCCTCAA GCTCCACGAC GTCTACGAGA ACAAGAAATA TTTGTAGGTA CCTGGTTCTG 
GAGCACGTCT CGGGGGGTGA GCTATTCGAC TACCTGGTAA AGAAGGGGAG ACTGACGCCC 
AAGGAGGCCC GAAAGTTCTT CCGCCAGATT GTGTCTGCGC TGGACTTCTG CCACAGCTAC 
TCCATCTGCC ACAGAGACCT AAAGCCCGAG AACCTGCTTT TGGATGAGAA AAACAACATC 
CGCATTGCAG ACTTCGGCAT GGCGTCCCTG CAGGTGGGGG ACAGCCTCCT GGAGACCAGC 
TGCGGGTCCC CCCATTATGC GTGTCCAGAG GTGATTAAGG GGGAAAAATA TGATGGCCGC 
CGGGCAGACA TGTGGAGCTG TGGAGTCATC CTCTTCGCCC TGCTCGTGGG GGCTCTGCCC 
TTTGATGACG ACAACCTCCG CCAGCTGCTG GAGAAGGTGA AACGGGGCGT CTTCCACATG 
CCCCACTTCA TTCCTCCAGA TTGCCAGAGC CTCCTGAGGG GAATGATCGA AGTGGAGCCC 
GAAAAAAGGC TCAGTCTGGA GCAAATTCAG AAACATCCTT GGTACCTAGG CGGGAAACAC 
GAGCCAGACC CGTGCCTGGA GCCAGCCCCT GGCCGCCGGG TAGCCATGCG GAGCCTGCCA 
TCCAACGGAG AGCTGGACCC CGACGTCCTA GAGAGCATGG CATCACTGGG CTGCTTCAGG 
GACCGCGAGA GGCTGCATCG CGAGCTGCGC AGTGAGGAGG AGAACCAAGA AAAGATGATA 
TATTATCTGC TTTTGGATCG GAAGGAGCGG TATCCCAGCT GTGAGGACCA GGACCTGCCT 
CCCCGGAATG ATGTTGACCC CCCCCGGAAG CGTGTGGATT CTCCCATGCT GAGCCGTCAC 
GGGAAGCGGC GACCAGAGCG GAAGTCCATG GAAGTCCTGA GCATCACCGA TGCCGGGGGT 
GGTGGCTCCC CTGTACCCAC CCGACGGGCC TTGGAGATGG CCCAGCACAG CCAGAGATCC 
CGTAGCGTCA GTGGAGCCTC CACGGGTCTG TCCTCCAGCC CTCTAAGCAG CCCAAGGAGT 
CCGGTCTTTT CCTTTTCACC GGAGCCGGGG GCTGGAGATG AGGCTCGAGG CGGGGGCTCC 
CCGACTTCCA AAACGCAGAC GCTGCCTTCT CGGGGCCCCA GGGGTGGGGG CGCCGGGGAG 
CAGCCCCCGC CCCCCAGTGC CCGCTCCACA CCCCTGCCCG GCCCCCCAGG CTCCCCGCGC 
TCCTCTGGCG GGACCCCCTT GCACTCGCCT CTGCACACGC CCCGGGCCAG TCCCACCGGG 
ACCCCGGGGA CAACACCACC CCCCAGCCCC GGCGGTGGCG TCGGGGGAGC CGCCTGGAGG 
AGTCGTCTCA ACTCCATCCG CAACAGCTTC CTGGGCTCCC CTCGCTTTCA CCGGCGCAAG 
ATGCAGGTCC CTACCGCTGA GGAGATGTCC AGCTTGACGC CAGAGTCCTC CCCGGAGCTG 
GCAAAACGCT CCTGGTTCGG GAACTTCATC TCCTTGGACA AAGAAGAACA AATATTCCTC 
GTGCTAAAGG ACAAACCTCT CAGCAGCATC AAAGCAGACA TCGTCCATGC CTTTCTGTCG 
ATCCCCAGCC TGAGTCACAG TGTGCTGTCA CAGACCAGCT TCAGGGCCGA GTACAAGGCC 
AGTGGCGGCC CCTCCGTCTT CCAAAAGCCC GTCCGCTTCC AGGTGGACAT CAGCTCCTCT 
GAGGGTCCAG AGCCCTCCCC GCGACGGGAC GGCAGCGGAG GTGGTGGCAT CTACTCCGTC 
ACCTTCACTC TCATCTCGGG TCCCAGCCGT CGGTTCAAGC GAGTGGTGGA GACCATCCAG 
GCACAGCTCC TGAGCACTCA TGACCAGCCC TCCGTGCAGG CCCTGGCAGA CGAGAAGAAC 
GGGGCCCAGA CCCGGCCTGC TGGTGCCCCA CCCCGAAGCC TGCAGCCCCC ACCCGGCCGC 
CCAGACCCAG AGCTGAGCAG CTCTCCCCGC CGAGGCCCCC CCAAGGACAA GAAGCTCCTG 
GCCACCAACG GGACCCCTCT GCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BRSK1 ( XM_011527395.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011525697.2 Homo sapiens BR serine/threonine kinase 1 (BRSK1), transcript variant X3, mRNA.

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CloneID	OHu88762
Clone ID Related Accession (Same CDS sequence)	XM_011527395.2
Accession Version	XM_011527395.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1809bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase BRSK1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011527395.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTCCC TGCAGGTGGG GGACAGCCTC CTGGAGACCA GCTGCGGGTC CCCCCATTAT 
GCGTGTCCAG AGGTGATTAA GGGGGAAAAA TATGATGGCC GCCGGGCAGA CATGTGGAGC 
TGTGGAGTCA TCCTCTTCGC CCTGCTCGTG GGGGCTCTGC CCTTTGATGA CGACAACCTC 
CGCCAGCTGC TGGAGAAGGT GAAACGGGGC GTCTTCCACA TGCCCCACTT CATTCCTCCA 
GATTGCCAGA GCCTCCTGAG GGGAATGATC GAAGTGGAGC CCGAAAAAAG GCTCAGTCTG 
GAGCAAATTC AGAAACATCC TTGGTACCTA GGCGGGAAAC ACGAGCCAGA CCCGTGCCTG 
GAGCCAGCCC CTGGCCGCCG GGTAGCCATG CGGAGCCTGC CATCCAACGG AGAGCTGGAC 
CCCGACGTCC TAGAGAGCAT GGCATCACTG GGCTGCTTCA GGGACCGCGA GAGGCTGCAT 
CGCGAGCTGC GCAGTGAGGA GGAGAACCAA GAAAAGATGA TATATTATCT GCTTTTGGAT 
CGGAAGGAGC GGTATCCCAG CTGTGAGGAC CAGGACCTGC CTCCCCGGAA TGATGTTGAC 
CCCCCCCGGA AGCGTGTGGA TTCTCCCATG CTGAGCCGTC ACGGGAAGCG GCGACCAGAG 
CGGAAGTCCA TGGAAGTCCT GAGCATCACC GATGCCGGGG GTGGTGGCTC CCCTGTACCC 
ACCCGACGGG CCTTGGAGAT GGCCCAGCAC AGCCAGAGAT CCCGTAGCGT CAGTGGAGCC 
TCCACGGGTC TGTCCTCCAG CCCTCTAAGC AGCCCAAGGA GTCCGGTCTT TTCCTTTTCA 
CCGGAGCCGG GGGCTGGAGA TGAGGCTCGA GGCGGGGGCT CCCCGACTTC CAAAACGCAG 
ACGCTGCCTT CTCGGGGCCC CAGGGGTGGG GGCGCCGGGG AGCAGCCCCC GCCCCCCAGT 
GCCCGCTCCA CACCCCTGCC CGGCCCCCCA GGCTCCCCGC GCTCCTCTGG CGGGACCCCC 
TTGCACTCGC CTCTGCACAC GCCCCGGGCC AGTCCCACCG GGACCCCGGG GACAACACCA 
CCCCCCAGCC CCGGCGGTGG CGTCGGGGGA GCCGCCTGGA GGAGTCGTCT CAACTCCATC 
CGCAACAGCT TCCTGGGCTC CCCTCGCTTT CACCGGCGCA AGATGCAGGT CCCTACCGCT 
GAGGAGATGT CCAGCTTGAC GCCAGAGTCC TCCCCGGAGC TGGCAAAACG CTCCTGGTTC 
GGGAACTTCA TCTCCTTGGA CAAAGAAGAA CAAATATTCC TCGTGCTAAA GGACAAACCT 
CTCAGCAGCA TCAAAGCAGA CATCGTCCAT GCCTTTCTGT CGATCCCCAG CCTGAGTCAC 
AGTGTGCTGT CACAGACCAG CTTCAGGGCC GAGTACAAGG CCAGTGGCGG CCCCTCCGTC 
TTCCAAAAGC CCGTCCGCTT CCAGGTGGAC ATCAGCTCCT CTGAGGGTCC AGAGCCCTCC 
CCGCGACGGG ACGGCAGCGG AGGTGGTGGC ATCTACTCCG TCACCTTCAC TCTCATCTCG 
GGTCCCAGCC GTCGGTTCAA GCGAGTGGTG GAGACCATCC AGGCACAGCT CCTGAGCACT 
CATGACCAGC CCTCCGTGCA GGCCCTGGCA GACGAGAAGA ACGGGGCCCA GACCCGGCCT 
GCTGGTGCCC CACCCCGAAG CCTGCAGCCC CCACCCGGCC GCCCAGACCC AGAGCTGAGC 
AGCTCTCCCC GCCGAGGCCC CCCCAAGGAC AAGAAGCTCC TGGCCACCAA CGGGACCCCT 
CTGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011525697.2
CDS	240..2048
Translation	MASLQVGDSLLETSCGSPHYACPEVIKGEKYDGRRADMWSCGVILFALLVGALPFDDDNLRQLLEKVKRGVFHMPHFIPPDCQSLLRGMIEVEPEKRLSLEQIQKHPWYLGGKHEPDPCLEPAPGRRVAMRSLPSNGELDPDVLESMASLGCFRDRERLHRELRSEEENQEKMIYYLLLDRKERYPSCEDQDLPPRNDVDPPRKRVDSPMLSRHGKRRPERKSMEVLSITDAGGGGSPVPTRRALEMAQHSQRSRSVSGASTGLSSSPLSSPRSPVFSFSPEPGAGDEARGGGSPTSKTQTLPSRGPRGGGAGEQPPPPSARSTPLPGPPGSPRSSGGTPLHSPLHTPRASPTGTPGTTPPPSPGGGVGGAAWRSRLNSIRNSFLGSPRFHRRKMQVPTAEEMSSLTPESSPELAKRSWFGNFISLDKEEQIFLVLKDKPLSSIKADIVHAFLSIPSLSHSVLSQTSFRAEYKASGGPSVFQKPVRFQVDISSSEGPEPSPRRDGSGGGGIYSVTFTLISGPSRRFKRVVETIQAQLLSTHDQPSVQALADEKNGAQTRPAGAPPRSLQPPPGRPDPELSSSPRRGPPKDKKLLATNGTPLP

Target ORF information:

RefSeq Version	XM_011527395.2
Organism	Homo sapiens(human)
Definition	Homo sapiens BR serine/threonine kinase 1 (BRSK1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011527395.2

ORF Insert Sequence:

ATGGCGTCCC TGCAGGTGGG GGACAGCCTC CTGGAGACCA GCTGCGGGTC CCCCCATTAT 
GCGTGTCCAG AGGTGATTAA GGGGGAAAAA TATGATGGCC GCCGGGCAGA CATGTGGAGC 
TGTGGAGTCA TCCTCTTCGC CCTGCTCGTG GGGGCTCTGC CCTTTGATGA CGACAACCTC 
CGCCAGCTGC TGGAGAAGGT GAAACGGGGC GTCTTCCACA TGCCCCACTT CATTCCTCCA 
GATTGCCAGA GCCTCCTGAG GGGAATGATC GAAGTGGAGC CCGAAAAAAG GCTCAGTCTG 
GAGCAAATTC AGAAACATCC TTGGTACCTA GGCGGGAAAC ACGAGCCAGA CCCGTGCCTG 
GAGCCAGCCC CTGGCCGCCG GGTAGCCATG CGGAGCCTGC CATCCAACGG AGAGCTGGAC 
CCCGACGTCC TAGAGAGCAT GGCATCACTG GGCTGCTTCA GGGACCGCGA GAGGCTGCAT 
CGCGAGCTGC GCAGTGAGGA GGAGAACCAA GAAAAGATGA TATATTATCT GCTTTTGGAT 
CGGAAGGAGC GGTATCCCAG CTGTGAGGAC CAGGACCTGC CTCCCCGGAA TGATGTTGAC 
CCCCCCCGGA AGCGTGTGGA TTCTCCCATG CTGAGCCGTC ACGGGAAGCG GCGACCAGAG 
CGGAAGTCCA TGGAAGTCCT GAGCATCACC GATGCCGGGG GTGGTGGCTC CCCTGTACCC 
ACCCGACGGG CCTTGGAGAT GGCCCAGCAC AGCCAGAGAT CCCGTAGCGT CAGTGGAGCC 
TCCACGGGTC TGTCCTCCAG CCCTCTAAGC AGCCCAAGGA GTCCGGTCTT TTCCTTTTCA 
CCGGAGCCGG GGGCTGGAGA TGAGGCTCGA GGCGGGGGCT CCCCGACTTC CAAAACGCAG 
ACGCTGCCTT CTCGGGGCCC CAGGGGTGGG GGCGCCGGGG AGCAGCCCCC GCCCCCCAGT 
GCCCGCTCCA CACCCCTGCC CGGCCCCCCA GGCTCCCCGC GCTCCTCTGG CGGGACCCCC 
TTGCACTCGC CTCTGCACAC GCCCCGGGCC AGTCCCACCG GGACCCCGGG GACAACACCA 
CCCCCCAGCC CCGGCGGTGG CGTCGGGGGA GCCGCCTGGA GGAGTCGTCT CAACTCCATC 
CGCAACAGCT TCCTGGGCTC CCCTCGCTTT CACCGGCGCA AGATGCAGGT CCCTACCGCT 
GAGGAGATGT CCAGCTTGAC GCCAGAGTCC TCCCCGGAGC TGGCAAAACG CTCCTGGTTC 
GGGAACTTCA TCTCCTTGGA CAAAGAAGAA CAAATATTCC TCGTGCTAAA GGACAAACCT 
CTCAGCAGCA TCAAAGCAGA CATCGTCCAT GCCTTTCTGT CGATCCCCAG CCTGAGTCAC 
AGTGTGCTGT CACAGACCAG CTTCAGGGCC GAGTACAAGG CCAGTGGCGG CCCCTCCGTC 
TTCCAAAAGC CCGTCCGCTT CCAGGTGGAC ATCAGCTCCT CTGAGGGTCC AGAGCCCTCC 
CCGCGACGGG ACGGCAGCGG AGGTGGTGGC ATCTACTCCG TCACCTTCAC TCTCATCTCG 
GGTCCCAGCC GTCGGTTCAA GCGAGTGGTG GAGACCATCC AGGCACAGCT CCTGAGCACT 
CATGACCAGC CCTCCGTGCA GGCCCTGGCA GACGAGAAGA ACGGGGCCCA GACCCGGCCT 
GCTGGTGCCC CACCCCGAAG CCTGCAGCCC CCACCCGGCC GCCCAGACCC AGAGCTGAGC 
AGCTCTCCCC GCCGAGGCCC CCCCAAGGAC AAGAAGCTCC TGGCCACCAA CGGGACCCCT 
CTGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Roles of tau protein in health and disease.
Acta neuropathologica133(5)665-704(2017 May)
Guo T,Noble W,Hanger DP

SAD-B Phosphorylation of CAST Controls Active Zone Vesicle Recycling for Synaptic Depression.
Cell reports16(11)2901-2913(2016 09)
Mochida S,Hida Y,Tanifuji S,Hagiwara A,Hamada S,Abe M,Ma H,Yasumura M,Kitajima I,Sakimura K,Ohtsuka T

Putative Tumor Suppressor Genes EGR1 and BRSK1 Are Mutated in Gastric and Colorectal Cancers.
Oncology91(5)289-294(2016)
Choi EJ,Yoo NJ,Kim MS,An CH,Lee SH

SAD-B kinase regulates pre-synaptic vesicular dynamics at hippocampal Schaffer collateral synapses and affects contextual fear memory.
Journal of neurochemistry136(1)36-47(2016 Jan)
Watabe AM,Nagase M,Hagiwara A,Hida Y,Tsuji M,Ochiai T,Kato F,Ohtsuka T

Decreased expression and prognostic role of cytoplasmic BRSK1 in human breast carcinoma: correlation with Jab1 stability and PI3K/Akt pathway.
Experimental and molecular pathology97(2)191-201(2014 Oct)
Wang H,Liu XB,Chen JH,Wang QQ,Chen JP,Xu JF,Sheng CY,Ni QC

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BRSK1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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