Egflam cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Egflam
Entrez Gene ID	365691
Full Name	EGF-like, fibronectin type III and laminin G domains
Synonyms	RGD1306592
General protein information	Preferred Names EGF-like, fibronectin type III and laminin G domains Names pikachurin EGF-like, fibronectin type-III and laminin G-like domain-containing protein
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q16

mRNA and Protein(s)

mRNA	Protein	Name
XM_017590987.1	XP_017446476.1	pikachurin isoform X3
XM_017590986.1	XP_017446475.1	pikachurin isoform X2
NM_001108938.2	NP_001102408.2	pikachurin precursor
XM_006232013.3	XP_006232075.1	pikachurin isoform X1
XM_008760781.2	XP_008759003.1	pikachurin isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	EGFLAM	NP_001192230.1
Caenorhabditis elegans (roundworm)	T19D12.6	NP_495350.4
Bos taurus (cattle)	EGFLAM	NP_001076947.1
Mus musculus (house mouse)	Egflam	NP_848863.1
Pan troglodytes (chimpanzee)	EGFLAM	XP_001142294.1
Canis lupus familiaris (dog)	EGFLAM	XP_005619433.1
Gallus gallus (chicken)	EGFLAM	XP_425019.4
Drosophila melanogaster (fruit fly)	SP2353	NP_611082.2
Xenopus tropicalis (tropical clawed frog)	egflam	XP_004910507.1
	EGFLAM	XP_001083161.1
Rattus norvegicus (Norway rat)	Egflam	NP_001102408.2

Gene Ontology
Bibliography

Related articles in PubMed

Cell specific post-translational processing of pikachurin, a protein involved in retinal synaptogenesis.
Han J, Townes-Anderson E
PloS one7(12)e50552(2012)

Transcriptome-based systematic identification of extracellular matrix proteins.
Manabe R, Tsutsui K, Yamada T, Kimura M, Nakano I, Shimono C, Sanzen N, Furutani Y, Fukuda T, Oguri Y, Shimamoto K, Kiyozumi D, Sato Y, Sado Y, Senoo H, Yamashina S, Fukuda S, Kawai J, Sugiura N, Kimata K, Hayashizaki Y, Sekiguchi K
Proceedings of the National Academy of Sciences of the United States of America105(35)12849-54(2008 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Egflam gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Egflam cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa48752	XM_017590986.1 Latest version!	Rattus norvegicus EGF-like, fibronectin type III and laminin G domains (Egflam), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa48752	XM_008760781.2 Latest version!	Rattus norvegicus EGF-like, fibronectin type III and laminin G domains (Egflam), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa59805	XM_017590987.1 Latest version!	Rattus norvegicus EGF-like, fibronectin type III and laminin G domains (Egflam), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ORa08691	NM_001108938.2 Latest version!	Rattus norvegicus EGF-like, fibronectin type III and laminin G domains (Egflam), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa24451	XM_006232013.3 Latest version!	Rattus norvegicus EGF-like, fibronectin type III and laminin G domains (Egflam), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Egflam ( XM_017590986.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017446475.1 Rattus norvegicus EGF-like, fibronectin type III and laminin G domains (Egflam), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa48752
Clone ID Related Accession (Same CDS sequence)	XM_017590986.1 , XM_008760781.2
Accession Version	XM_017590986.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3000bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	pikachurin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCCCC GTCCCCAATA TGACTACTTG CAGTTCATTC TGTTCCTTTT TGCAGGCAAG 
GTGGGACCTC CTCTTGACAT CAAACTGGGA GCCTTGAACT GTACAGCCTT CAGCATCCAG 
TGGAAAACAC CAAAGCGTTC TGGTAGCTCC ATTGTTGGGT ACACAGTATT TTACTCTGAG 
CTTGGTTCAG ATAAGTCCCT CCGGGAACAG TCACATAATG TACCTGTTGG CCAGGATACC 
CTAATCACTG AGGAAGTGAT TGGAGATTTG AAGCCAGGCA CTGAATATCG AGTGAGCATA 
GCAGCCTACA GCCAGACTGG CAAAGGGAGG CTTAGCTTCC CTCGGCATGT GACCACTTTG 
TCTCAAGATT CCTGCCTGCC CCCTGAAGCA CCCCATCAGC CACATGTCCT TGTGGTTTCT 
GATTCTGAAG TGGCCCTATC TTGGAGACCG GGAGAGAATG AAGGAAGTGC CCCAATTCAG 
TCCTACTCTG TGGAATTCAT TAGGCCAGAT TTTGACAAGA GCTGGACCAT AATCCAAGAG 
CGCCTGCAGA TGGACTCCAT GGTTATCAAA GGCCTTGATC CAGACACCAA CTACCAGTTT 
GCTGTGAGAG CCATGAATGC CTATGGCTTC AGTCTGCGCA GCCAGCCCAG CAACACCATC 
CGAACTCTGG GTCCTGGGGA GGCTGGAAGT GGCCGCTATG GACCTGGGTA TATCACCGAT 
ACCGGCGTGA GTGAGGATGA CGATGCATCA GAAGACGAGC TAGATCTGGA TGTCTCCTTT 
GAGGAGGTTA AACCACTTCC TGCTACCAAA GTTGGGAATA AGAAGTCCAA GAAGACTTCT 
GTCTCCAACT CAGAGATGGA CTCCAGACTT GCCCAACCTA CCTCAGCATC TCTCCCTGAA 
ACTACAGTGG CTGTGCCACC CACGCCAGCC CAACGGAAGG GGAAGAACAG TGTGGCCGTG 
ATGTCAAGGC TCTTTGACAT GTCCTGTGAC GAAACCCTCT GTTCAGCTGA CAGCTTCTGT 
GTCAATGACT ACGCCTGGGG AGGCTCACGA TGCCATTGCA ACCTGGGCAA AGGAGGCGAG 
GCCTGCTCAG AAGATATCTT TATTCAGTAT CCACAGTTCT TTGGCCACTC CTATGTAACC 
TTTGAGCCTC TGAAGAACTC TTATCAGGCA TTTCAGATTA CTCTGGAATT CAGGGCAGAG 
GCAGAAGATG GCTTACTGCT CTACTGTGGG GAGAGTGAAC ATGGGAGAGG AGACTTCATG 
TCCCTGGCTC TCATCCGACG CTCCCTCCAC TTCAGGTTTA ATTGTGGAAC TGGGATGGCC 
ATCATCATAA GTGAGACCAA AATCAAGCTT GGGGCCTGGC ACTCGGTGAC ACTGTATAGA 
GATGGGCTGA ACGGGCTGCT GCAGTTGAAC AATGGCACCC CAGTAACAGG CCAGTCCCAG 
GGTCAGTACA GTAAAATTAC CTTCCGGACA CCTCTGTACC TTGGTGGTGC TCCCAGTGCT 
TACTGGCTGG TCAGAGCGAC GGGGACAAAT CGTGGCTTCC AAGGCTGTGT GCAGTCACTT 
GCTGTTAACG GGAAGAAGAT TGACATGAGG CCCTGGCCAC TGGGGAAAGC CCTCAACGGA 
GCTGATGTTG GGGAGTGCAG CAGTGGGATC TGTGATGAAG CCTCCTGCAT CAACGGGGGC 
ACCTGCGCAG CCATCAAGGC CGACTCCTAC ATCTGCCTCT GTCCCCTTGG GTTCAGAGGT 
CGACACTGTG AAGATGCATT TACCCTGACC ATTCCTCAGT TCAGAGAGTC GCTGAGGTCC 
TACGCTGCCA CGCCCTGGCC CCTGGAGCCT CAGCACTATC TTTCTTTCAC CGAGTTTGAG 
ATCACATTTC GGCCAGACTC CGGGGATGGG GTCCTTTTGT ACAGCTACGA CACAAGCAGC 
AAGGACTTCC TGTCCATTAT TATGGCTGCA GGCCATGTGG AATTCCGCTT CGACTGTGGT 
TCTGGGACCG GAGTTCTCAG GAGTGAGGAT ACCCTCACCC TAGGCCAATG GCATGACCTA 
CGCGTGTCTC GCACAGCGAA GAATGGGATC TTACAGGTGG ACAAGCAGAA GGTAGTAGAA 
GGAATGGCAG AGGGAGGCTT CACCCAGATT AAGTGTAATA CAGACATTTT TATTGGCGGA 
GTCCCAAATT ACGATGATGT CAAGAAGAAC TCGGGTATCC TCCATCCATT TAGTGGGAGC 
ATCCAGAAGA TCATCCTAAA TGACCGGACC ATCCATGTGA GGCACGACTT TACATCTGGT 
GTGAATGTGG AGAACGCAGC GCACCCCTGT GTGGGGGCCC CCTGTGCCCA TGGGGGCAGC 
TGTAGGCCCA GGAAGGAGGG TTATGAGTGT GACTGTCCCT TGGGCTTCGA AGGCCTGAAC 
TGCCAGAAAG AGTGTGGAAA CTACTGCCTC AATACGATTA CAGAAGCCAT TGAGATCCCA 
CAGTTCATCG GTCGGAGTTA CCTGACCTAT GACAACCCAA ATATCCTCAA GAGGGTGTCC 
GGATCAAGGT CAAATGCTTT CATGAGGTTT AAGACAACCG CCAAGGACGG CCTGTTGCTG 
TGGAGGGGGG ACAGCCCCAT GAGACCTAAC AGCGACTTCA TTTCCCTGGG TCTTCGGGAT 
GGAGCCCTGG TGTTCAGCTA CAACCTGGGC AGTGGTGTGG CTTCCATTAT GGTGAATGGT 
TCCTTCAGCG ATGGCCGGTG GCACCGAGTC AAGGCTGTCA GGGATGGCCA GTCTGGGAAA 
ATTACGGTGG ATGACTATGG AGCCAGAACA GGCAAGTCAC CTGGCATGAT GAGGCAACTT 
AACATCAATG GGGCTCTGTA TGTGGGTGGA ATGAAGGAAA TTGCTCTGCA CACGAACAGA 
CAGTACATGC GAGGCCTTGT GGGCTGCATC TCCCATTTCA CATTGTCCAC CGATTACCAC 
ATTTCCCTTG TGGAAGACGC TGTGGATGGG AAGAACATCA ACACTTGTGG GGCTAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017446475.1
CDS	3651..6650
Translation	MQPRPQYDYLQFILFLFAGKVGPPLDIKLGALNCTAFSIQWKTPKRSGSSIVGYTVFYSELGSDKSLREQSHNVPVGQDTLITEEVIGDLKPGTEYRVSIAAYSQTGKGRLSFPRHVTTLSQDSCLPPEAPHQPHVLVVSDSEVALSWRPGENEGSAPIQSYSVEFIRPDFDKSWTIIQERLQMDSMVIKGLDPDTNYQFAVRAMNAYGFSLRSQPSNTIRTLGPGEAGSGRYGPGYITDTGVSEDDDASEDELDLDVSFEEVKPLPATKVGNKKSKKTSVSNSEMDSRLAQPTSASLPETTVAVPPTPAQRKGKNSVAVMSRLFDMSCDETLCSADSFCVNDYAWGGSRCHCNLGKGGEACSEDIFIQYPQFFGHSYVTFEPLKNSYQAFQITLEFRAEAEDGLLLYCGESEHGRGDFMSLALIRRSLHFRFNCGTGMAIIISETKIKLGAWHSVTLYRDGLNGLLQLNNGTPVTGQSQGQYSKITFRTPLYLGGAPSAYWLVRATGTNRGFQGCVQSLAVNGKKIDMRPWPLGKALNGADVGECSSGICDEASCINGGTCAAIKADSYICLCPLGFRGRHCEDAFTLTIPQFRESLRSYAATPWPLEPQHYLSFTEFEITFRPDSGDGVLLYSYDTSSKDFLSIIMAAGHVEFRFDCGSGTGVLRSEDTLTLGQWHDLRVSRTAKNGILQVDKQKVVEGMAEGGFTQIKCNTDIFIGGVPNYDDVKKNSGILHPFSGSIQKIILNDRTIHVRHDFTSGVNVENAAHPCVGAPCAHGGSCRPRKEGYECDCPLGFEGLNCQKECGNYCLNTITEAIEIPQFIGRSYLTYDNPNILKRVSGSRSNAFMRFKTTAKDGLLLWRGDSPMRPNSDFISLGLRDGALVFSYNLGSGVASIMVNGSFSDGRWHRVKAVRDGQSGKITVDDYGARTGKSPGMMRQLNINGALYVGGMKEIALHTNRQYMRGLVGCISHFTLSTDYHISLVEDAVDGKNINTCGAK

Target ORF information:

RefSeq Version	XM_017590986.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus EGF-like, fibronectin type III and laminin G domains (Egflam), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017590986.1

ORF Insert Sequence:

ATGCAGCCCC GTCCCCAATA TGACTACTTG CAGTTCATTC TGTTCCTTTT TGCAGGCAAG 
GTGGGACCTC CTCTTGACAT CAAACTGGGA GCCTTGAACT GTACAGCCTT CAGCATCCAG 
TGGAAAACAC CAAAGCGTTC TGGTAGCTCC ATTGTTGGGT ACACAGTATT TTACTCTGAG 
CTTGGTTCAG ATAAGTCCCT CCGGGAACAG TCACATAATG TACCTGTTGG CCAGGATACC 
CTAATCACTG AGGAAGTGAT TGGAGATTTG AAGCCAGGCA CTGAATATCG AGTGAGCATA 
GCAGCCTACA GCCAGACTGG CAAAGGGAGG CTTAGCTTCC CTCGGCATGT GACCACTTTG 
TCTCAAGATT CCTGCCTGCC CCCTGAAGCA CCCCATCAGC CACATGTCCT TGTGGTTTCT 
GATTCTGAAG TGGCCCTATC TTGGAGACCG GGAGAGAATG AAGGAAGTGC CCCAATTCAG 
TCCTACTCTG TGGAATTCAT TAGGCCAGAT TTTGACAAGA GCTGGACCAT AATCCAAGAG 
CGCCTGCAGA TGGACTCCAT GGTTATCAAA GGCCTTGATC CAGACACCAA CTACCAGTTT 
GCTGTGAGAG CCATGAATGC CTATGGCTTC AGTCTGCGCA GCCAGCCCAG CAACACCATC 
CGAACTCTGG GTCCTGGGGA GGCTGGAAGT GGCCGCTATG GACCTGGGTA TATCACCGAT 
ACCGGCGTGA GTGAGGATGA CGATGCATCA GAAGACGAGC TAGATCTGGA TGTCTCCTTT 
GAGGAGGTTA AACCACTTCC TGCTACCAAA GTTGGGAATA AGAAGTCCAA GAAGACTTCT 
GTCTCCAACT CAGAGATGGA CTCCAGACTT GCCCAACCTA CCTCAGCATC TCTCCCTGAA 
ACTACAGTGG CTGTGCCACC CACGCCAGCC CAACGGAAGG GGAAGAACAG TGTGGCCGTG 
ATGTCAAGGC TCTTTGACAT GTCCTGTGAC GAAACCCTCT GTTCAGCTGA CAGCTTCTGT 
GTCAATGACT ACGCCTGGGG AGGCTCACGA TGCCATTGCA ACCTGGGCAA AGGAGGCGAG 
GCCTGCTCAG AAGATATCTT TATTCAGTAT CCACAGTTCT TTGGCCACTC CTATGTAACC 
TTTGAGCCTC TGAAGAACTC TTATCAGGCA TTTCAGATTA CTCTGGAATT CAGGGCAGAG 
GCAGAAGATG GCTTACTGCT CTACTGTGGG GAGAGTGAAC ATGGGAGAGG AGACTTCATG 
TCCCTGGCTC TCATCCGACG CTCCCTCCAC TTCAGGTTTA ATTGTGGAAC TGGGATGGCC 
ATCATCATAA GTGAGACCAA AATCAAGCTT GGGGCCTGGC ACTCGGTGAC ACTGTATAGA 
GATGGGCTGA ACGGGCTGCT GCAGTTGAAC AATGGCACCC CAGTAACAGG CCAGTCCCAG 
GGTCAGTACA GTAAAATTAC CTTCCGGACA CCTCTGTACC TTGGTGGTGC TCCCAGTGCT 
TACTGGCTGG TCAGAGCGAC GGGGACAAAT CGTGGCTTCC AAGGCTGTGT GCAGTCACTT 
GCTGTTAACG GGAAGAAGAT TGACATGAGG CCCTGGCCAC TGGGGAAAGC CCTCAACGGA 
GCTGATGTTG GGGAGTGCAG CAGTGGGATC TGTGATGAAG CCTCCTGCAT CAACGGGGGC 
ACCTGCGCAG CCATCAAGGC CGACTCCTAC ATCTGCCTCT GTCCCCTTGG GTTCAGAGGT 
CGACACTGTG AAGATGCATT TACCCTGACC ATTCCTCAGT TCAGAGAGTC GCTGAGGTCC 
TACGCTGCCA CGCCCTGGCC CCTGGAGCCT CAGCACTATC TTTCTTTCAC CGAGTTTGAG 
ATCACATTTC GGCCAGACTC CGGGGATGGG GTCCTTTTGT ACAGCTACGA CACAAGCAGC 
AAGGACTTCC TGTCCATTAT TATGGCTGCA GGCCATGTGG AATTCCGCTT CGACTGTGGT 
TCTGGGACCG GAGTTCTCAG GAGTGAGGAT ACCCTCACCC TAGGCCAATG GCATGACCTA 
CGCGTGTCTC GCACAGCGAA GAATGGGATC TTACAGGTGG ACAAGCAGAA GGTAGTAGAA 
GGAATGGCAG AGGGAGGCTT CACCCAGATT AAGTGTAATA CAGACATTTT TATTGGCGGA 
GTCCCAAATT ACGATGATGT CAAGAAGAAC TCGGGTATCC TCCATCCATT TAGTGGGAGC 
ATCCAGAAGA TCATCCTAAA TGACCGGACC ATCCATGTGA GGCACGACTT TACATCTGGT 
GTGAATGTGG AGAACGCAGC GCACCCCTGT GTGGGGGCCC CCTGTGCCCA TGGGGGCAGC 
TGTAGGCCCA GGAAGGAGGG TTATGAGTGT GACTGTCCCT TGGGCTTCGA AGGCCTGAAC 
TGCCAGAAAG AGTGTGGAAA CTACTGCCTC AATACGATTA CAGAAGCCAT TGAGATCCCA 
CAGTTCATCG GTCGGAGTTA CCTGACCTAT GACAACCCAA ATATCCTCAA GAGGGTGTCC 
GGATCAAGGT CAAATGCTTT CATGAGGTTT AAGACAACCG CCAAGGACGG CCTGTTGCTG 
TGGAGGGGGG ACAGCCCCAT GAGACCTAAC AGCGACTTCA TTTCCCTGGG TCTTCGGGAT 
GGAGCCCTGG TGTTCAGCTA CAACCTGGGC AGTGGTGTGG CTTCCATTAT GGTGAATGGT 
TCCTTCAGCG ATGGCCGGTG GCACCGAGTC AAGGCTGTCA GGGATGGCCA GTCTGGGAAA 
ATTACGGTGG ATGACTATGG AGCCAGAACA GGCAAGTCAC CTGGCATGAT GAGGCAACTT 
AACATCAATG GGGCTCTGTA TGTGGGTGGA ATGAAGGAAA TTGCTCTGCA CACGAACAGA 
CAGTACATGC GAGGCCTTGT GGGCTGCATC TCCCATTTCA CATTGTCCAC CGATTACCAC 
ATTTCCCTTG TGGAAGACGC TGTGGATGGG AAGAACATCA ACACTTGTGG GGCTAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Egflam ( XM_008760781.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008759003.1 Rattus norvegicus EGF-like, fibronectin type III and laminin G domains (Egflam), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa48752
Clone ID Related Accession (Same CDS sequence)	XM_017590986.1 , XM_008760781.2
Accession Version	XM_008760781.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3000bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	pikachurin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008760781.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCCCC GTCCCCAATA TGACTACTTG CAGTTCATTC TGTTCCTTTT TGCAGGCAAG 
GTGGGACCTC CTCTTGACAT CAAACTGGGA GCCTTGAACT GTACAGCCTT CAGCATCCAG 
TGGAAAACAC CAAAGCGTTC TGGTAGCTCC ATTGTTGGGT ACACAGTATT TTACTCTGAG 
CTTGGTTCAG ATAAGTCCCT CCGGGAACAG TCACATAATG TACCTGTTGG CCAGGATACC 
CTAATCACTG AGGAAGTGAT TGGAGATTTG AAGCCAGGCA CTGAATATCG AGTGAGCATA 
GCAGCCTACA GCCAGACTGG CAAAGGGAGG CTTAGCTTCC CTCGGCATGT GACCACTTTG 
TCTCAAGATT CCTGCCTGCC CCCTGAAGCA CCCCATCAGC CACATGTCCT TGTGGTTTCT 
GATTCTGAAG TGGCCCTATC TTGGAGACCG GGAGAGAATG AAGGAAGTGC CCCAATTCAG 
TCCTACTCTG TGGAATTCAT TAGGCCAGAT TTTGACAAGA GCTGGACCAT AATCCAAGAG 
CGCCTGCAGA TGGACTCCAT GGTTATCAAA GGCCTTGATC CAGACACCAA CTACCAGTTT 
GCTGTGAGAG CCATGAATGC CTATGGCTTC AGTCTGCGCA GCCAGCCCAG CAACACCATC 
CGAACTCTGG GTCCTGGGGA GGCTGGAAGT GGCCGCTATG GACCTGGGTA TATCACCGAT 
ACCGGCGTGA GTGAGGATGA CGATGCATCA GAAGACGAGC TAGATCTGGA TGTCTCCTTT 
GAGGAGGTTA AACCACTTCC TGCTACCAAA GTTGGGAATA AGAAGTCCAA GAAGACTTCT 
GTCTCCAACT CAGAGATGGA CTCCAGACTT GCCCAACCTA CCTCAGCATC TCTCCCTGAA 
ACTACAGTGG CTGTGCCACC CACGCCAGCC CAACGGAAGG GGAAGAACAG TGTGGCCGTG 
ATGTCAAGGC TCTTTGACAT GTCCTGTGAC GAAACCCTCT GTTCAGCTGA CAGCTTCTGT 
GTCAATGACT ACGCCTGGGG AGGCTCACGA TGCCATTGCA ACCTGGGCAA AGGAGGCGAG 
GCCTGCTCAG AAGATATCTT TATTCAGTAT CCACAGTTCT TTGGCCACTC CTATGTAACC 
TTTGAGCCTC TGAAGAACTC TTATCAGGCA TTTCAGATTA CTCTGGAATT CAGGGCAGAG 
GCAGAAGATG GCTTACTGCT CTACTGTGGG GAGAGTGAAC ATGGGAGAGG AGACTTCATG 
TCCCTGGCTC TCATCCGACG CTCCCTCCAC TTCAGGTTTA ATTGTGGAAC TGGGATGGCC 
ATCATCATAA GTGAGACCAA AATCAAGCTT GGGGCCTGGC ACTCGGTGAC ACTGTATAGA 
GATGGGCTGA ACGGGCTGCT GCAGTTGAAC AATGGCACCC CAGTAACAGG CCAGTCCCAG 
GGTCAGTACA GTAAAATTAC CTTCCGGACA CCTCTGTACC TTGGTGGTGC TCCCAGTGCT 
TACTGGCTGG TCAGAGCGAC GGGGACAAAT CGTGGCTTCC AAGGCTGTGT GCAGTCACTT 
GCTGTTAACG GGAAGAAGAT TGACATGAGG CCCTGGCCAC TGGGGAAAGC CCTCAACGGA 
GCTGATGTTG GGGAGTGCAG CAGTGGGATC TGTGATGAAG CCTCCTGCAT CAACGGGGGC 
ACCTGCGCAG CCATCAAGGC CGACTCCTAC ATCTGCCTCT GTCCCCTTGG GTTCAGAGGT 
CGACACTGTG AAGATGCATT TACCCTGACC ATTCCTCAGT TCAGAGAGTC GCTGAGGTCC 
TACGCTGCCA CGCCCTGGCC CCTGGAGCCT CAGCACTATC TTTCTTTCAC CGAGTTTGAG 
ATCACATTTC GGCCAGACTC CGGGGATGGG GTCCTTTTGT ACAGCTACGA CACAAGCAGC 
AAGGACTTCC TGTCCATTAT TATGGCTGCA GGCCATGTGG AATTCCGCTT CGACTGTGGT 
TCTGGGACCG GAGTTCTCAG GAGTGAGGAT ACCCTCACCC TAGGCCAATG GCATGACCTA 
CGCGTGTCTC GCACAGCGAA GAATGGGATC TTACAGGTGG ACAAGCAGAA GGTAGTAGAA 
GGAATGGCAG AGGGAGGCTT CACCCAGATT AAGTGTAATA CAGACATTTT TATTGGCGGA 
GTCCCAAATT ACGATGATGT CAAGAAGAAC TCGGGTATCC TCCATCCATT TAGTGGGAGC 
ATCCAGAAGA TCATCCTAAA TGACCGGACC ATCCATGTGA GGCACGACTT TACATCTGGT 
GTGAATGTGG AGAACGCAGC GCACCCCTGT GTGGGGGCCC CCTGTGCCCA TGGGGGCAGC 
TGTAGGCCCA GGAAGGAGGG TTATGAGTGT GACTGTCCCT TGGGCTTCGA AGGCCTGAAC 
TGCCAGAAAG AGTGTGGAAA CTACTGCCTC AATACGATTA CAGAAGCCAT TGAGATCCCA 
CAGTTCATCG GTCGGAGTTA CCTGACCTAT GACAACCCAA ATATCCTCAA GAGGGTGTCC 
GGATCAAGGT CAAATGCTTT CATGAGGTTT AAGACAACCG CCAAGGACGG CCTGTTGCTG 
TGGAGGGGGG ACAGCCCCAT GAGACCTAAC AGCGACTTCA TTTCCCTGGG TCTTCGGGAT 
GGAGCCCTGG TGTTCAGCTA CAACCTGGGC AGTGGTGTGG CTTCCATTAT GGTGAATGGT 
TCCTTCAGCG ATGGCCGGTG GCACCGAGTC AAGGCTGTCA GGGATGGCCA GTCTGGGAAA 
ATTACGGTGG ATGACTATGG AGCCAGAACA GGCAAGTCAC CTGGCATGAT GAGGCAACTT 
AACATCAATG GGGCTCTGTA TGTGGGTGGA ATGAAGGAAA TTGCTCTGCA CACGAACAGA 
CAGTACATGC GAGGCCTTGT GGGCTGCATC TCCCATTTCA CATTGTCCAC CGATTACCAC 
ATTTCCCTTG TGGAAGACGC TGTGGATGGG AAGAACATCA ACACTTGTGG GGCTAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008759003.1
CDS	3734..6733
Translation	MQPRPQYDYLQFILFLFAGKVGPPLDIKLGALNCTAFSIQWKTPKRSGSSIVGYTVFYSELGSDKSLREQSHNVPVGQDTLITEEVIGDLKPGTEYRVSIAAYSQTGKGRLSFPRHVTTLSQDSCLPPEAPHQPHVLVVSDSEVALSWRPGENEGSAPIQSYSVEFIRPDFDKSWTIIQERLQMDSMVIKGLDPDTNYQFAVRAMNAYGFSLRSQPSNTIRTLGPGEAGSGRYGPGYITDTGVSEDDDASEDELDLDVSFEEVKPLPATKVGNKKSKKTSVSNSEMDSRLAQPTSASLPETTVAVPPTPAQRKGKNSVAVMSRLFDMSCDETLCSADSFCVNDYAWGGSRCHCNLGKGGEACSEDIFIQYPQFFGHSYVTFEPLKNSYQAFQITLEFRAEAEDGLLLYCGESEHGRGDFMSLALIRRSLHFRFNCGTGMAIIISETKIKLGAWHSVTLYRDGLNGLLQLNNGTPVTGQSQGQYSKITFRTPLYLGGAPSAYWLVRATGTNRGFQGCVQSLAVNGKKIDMRPWPLGKALNGADVGECSSGICDEASCINGGTCAAIKADSYICLCPLGFRGRHCEDAFTLTIPQFRESLRSYAATPWPLEPQHYLSFTEFEITFRPDSGDGVLLYSYDTSSKDFLSIIMAAGHVEFRFDCGSGTGVLRSEDTLTLGQWHDLRVSRTAKNGILQVDKQKVVEGMAEGGFTQIKCNTDIFIGGVPNYDDVKKNSGILHPFSGSIQKIILNDRTIHVRHDFTSGVNVENAAHPCVGAPCAHGGSCRPRKEGYECDCPLGFEGLNCQKECGNYCLNTITEAIEIPQFIGRSYLTYDNPNILKRVSGSRSNAFMRFKTTAKDGLLLWRGDSPMRPNSDFISLGLRDGALVFSYNLGSGVASIMVNGSFSDGRWHRVKAVRDGQSGKITVDDYGARTGKSPGMMRQLNINGALYVGGMKEIALHTNRQYMRGLVGCISHFTLSTDYHISLVEDAVDGKNINTCGAK

Target ORF information:

RefSeq Version	XM_008760781.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus EGF-like, fibronectin type III and laminin G domains (Egflam), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008760781.2

ORF Insert Sequence:

ATGCAGCCCC GTCCCCAATA TGACTACTTG CAGTTCATTC TGTTCCTTTT TGCAGGCAAG 
GTGGGACCTC CTCTTGACAT CAAACTGGGA GCCTTGAACT GTACAGCCTT CAGCATCCAG 
TGGAAAACAC CAAAGCGTTC TGGTAGCTCC ATTGTTGGGT ACACAGTATT TTACTCTGAG 
CTTGGTTCAG ATAAGTCCCT CCGGGAACAG TCACATAATG TACCTGTTGG CCAGGATACC 
CTAATCACTG AGGAAGTGAT TGGAGATTTG AAGCCAGGCA CTGAATATCG AGTGAGCATA 
GCAGCCTACA GCCAGACTGG CAAAGGGAGG CTTAGCTTCC CTCGGCATGT GACCACTTTG 
TCTCAAGATT CCTGCCTGCC CCCTGAAGCA CCCCATCAGC CACATGTCCT TGTGGTTTCT 
GATTCTGAAG TGGCCCTATC TTGGAGACCG GGAGAGAATG AAGGAAGTGC CCCAATTCAG 
TCCTACTCTG TGGAATTCAT TAGGCCAGAT TTTGACAAGA GCTGGACCAT AATCCAAGAG 
CGCCTGCAGA TGGACTCCAT GGTTATCAAA GGCCTTGATC CAGACACCAA CTACCAGTTT 
GCTGTGAGAG CCATGAATGC CTATGGCTTC AGTCTGCGCA GCCAGCCCAG CAACACCATC 
CGAACTCTGG GTCCTGGGGA GGCTGGAAGT GGCCGCTATG GACCTGGGTA TATCACCGAT 
ACCGGCGTGA GTGAGGATGA CGATGCATCA GAAGACGAGC TAGATCTGGA TGTCTCCTTT 
GAGGAGGTTA AACCACTTCC TGCTACCAAA GTTGGGAATA AGAAGTCCAA GAAGACTTCT 
GTCTCCAACT CAGAGATGGA CTCCAGACTT GCCCAACCTA CCTCAGCATC TCTCCCTGAA 
ACTACAGTGG CTGTGCCACC CACGCCAGCC CAACGGAAGG GGAAGAACAG TGTGGCCGTG 
ATGTCAAGGC TCTTTGACAT GTCCTGTGAC GAAACCCTCT GTTCAGCTGA CAGCTTCTGT 
GTCAATGACT ACGCCTGGGG AGGCTCACGA TGCCATTGCA ACCTGGGCAA AGGAGGCGAG 
GCCTGCTCAG AAGATATCTT TATTCAGTAT CCACAGTTCT TTGGCCACTC CTATGTAACC 
TTTGAGCCTC TGAAGAACTC TTATCAGGCA TTTCAGATTA CTCTGGAATT CAGGGCAGAG 
GCAGAAGATG GCTTACTGCT CTACTGTGGG GAGAGTGAAC ATGGGAGAGG AGACTTCATG 
TCCCTGGCTC TCATCCGACG CTCCCTCCAC TTCAGGTTTA ATTGTGGAAC TGGGATGGCC 
ATCATCATAA GTGAGACCAA AATCAAGCTT GGGGCCTGGC ACTCGGTGAC ACTGTATAGA 
GATGGGCTGA ACGGGCTGCT GCAGTTGAAC AATGGCACCC CAGTAACAGG CCAGTCCCAG 
GGTCAGTACA GTAAAATTAC CTTCCGGACA CCTCTGTACC TTGGTGGTGC TCCCAGTGCT 
TACTGGCTGG TCAGAGCGAC GGGGACAAAT CGTGGCTTCC AAGGCTGTGT GCAGTCACTT 
GCTGTTAACG GGAAGAAGAT TGACATGAGG CCCTGGCCAC TGGGGAAAGC CCTCAACGGA 
GCTGATGTTG GGGAGTGCAG CAGTGGGATC TGTGATGAAG CCTCCTGCAT CAACGGGGGC 
ACCTGCGCAG CCATCAAGGC CGACTCCTAC ATCTGCCTCT GTCCCCTTGG GTTCAGAGGT 
CGACACTGTG AAGATGCATT TACCCTGACC ATTCCTCAGT TCAGAGAGTC GCTGAGGTCC 
TACGCTGCCA CGCCCTGGCC CCTGGAGCCT CAGCACTATC TTTCTTTCAC CGAGTTTGAG 
ATCACATTTC GGCCAGACTC CGGGGATGGG GTCCTTTTGT ACAGCTACGA CACAAGCAGC 
AAGGACTTCC TGTCCATTAT TATGGCTGCA GGCCATGTGG AATTCCGCTT CGACTGTGGT 
TCTGGGACCG GAGTTCTCAG GAGTGAGGAT ACCCTCACCC TAGGCCAATG GCATGACCTA 
CGCGTGTCTC GCACAGCGAA GAATGGGATC TTACAGGTGG ACAAGCAGAA GGTAGTAGAA 
GGAATGGCAG AGGGAGGCTT CACCCAGATT AAGTGTAATA CAGACATTTT TATTGGCGGA 
GTCCCAAATT ACGATGATGT CAAGAAGAAC TCGGGTATCC TCCATCCATT TAGTGGGAGC 
ATCCAGAAGA TCATCCTAAA TGACCGGACC ATCCATGTGA GGCACGACTT TACATCTGGT 
GTGAATGTGG AGAACGCAGC GCACCCCTGT GTGGGGGCCC CCTGTGCCCA TGGGGGCAGC 
TGTAGGCCCA GGAAGGAGGG TTATGAGTGT GACTGTCCCT TGGGCTTCGA AGGCCTGAAC 
TGCCAGAAAG AGTGTGGAAA CTACTGCCTC AATACGATTA CAGAAGCCAT TGAGATCCCA 
CAGTTCATCG GTCGGAGTTA CCTGACCTAT GACAACCCAA ATATCCTCAA GAGGGTGTCC 
GGATCAAGGT CAAATGCTTT CATGAGGTTT AAGACAACCG CCAAGGACGG CCTGTTGCTG 
TGGAGGGGGG ACAGCCCCAT GAGACCTAAC AGCGACTTCA TTTCCCTGGG TCTTCGGGAT 
GGAGCCCTGG TGTTCAGCTA CAACCTGGGC AGTGGTGTGG CTTCCATTAT GGTGAATGGT 
TCCTTCAGCG ATGGCCGGTG GCACCGAGTC AAGGCTGTCA GGGATGGCCA GTCTGGGAAA 
ATTACGGTGG ATGACTATGG AGCCAGAACA GGCAAGTCAC CTGGCATGAT GAGGCAACTT 
AACATCAATG GGGCTCTGTA TGTGGGTGGA ATGAAGGAAA TTGCTCTGCA CACGAACAGA 
CAGTACATGC GAGGCCTTGT GGGCTGCATC TCCCATTTCA CATTGTCCAC CGATTACCAC 
ATTTCCCTTG TGGAAGACGC TGTGGATGGG AAGAACATCA ACACTTGTGG GGCTAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Egflam ( XM_017590987.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017446476.1 Rattus norvegicus EGF-like, fibronectin type III and laminin G domains (Egflam), transcript variant X4, mRNA.

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CloneID	ORa59805
Clone ID Related Accession (Same CDS sequence)	XM_017590987.1
Accession Version	XM_017590987.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2592bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	pikachurin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCTTGT GGTTTCTGAT TCTGAAGTGG CCCTATCTTG GAGACCGGGA GAGAATGAAG 
GAAGTGCCCC AATTCAGTCC TACTCTGTGG AATTCATTAG ATTTTGACAA GAGCTGGACC 
ATAATCCAAG AGCGCCTGCA GATGGACTCC ATGGTTATCA AAGGCCTTGA TCCAGACACC 
AACTACCAGT TTGCTGTGAG AGCCATGAAT GCCTATGGCT TCAGTCTGCG CAGCCAGCCC 
AGCAACACCA TCCGAACTCT GGGTCCTGGG GAGGCTGGAA GTGGCCGCTA TGGACCTGGG 
TATATCACCG ATACCGGCGT GAGTGAGGAT GACGATGCAT CAGAAGACGA GCTAGATCTG 
GATGTCTCCT TTGAGGAGGT TAAACCACTT CCTGCTACCA AAGTTGGGAA TAAGAAGTCC 
AAGAAGACTT CTGTCTCCAA CTCAGAGATG GACTCCAGAC TTGCCCAACC TACCTCAGCA 
TCTCTCCCTG AAACTACAGT GGCTGTGCCA CCCACGCCAG CCCAACGGAA GGGGAAGAAC 
AGTGTGGCCG TGATGTCAAG GCTCTTTGAC ATGTCCTGTG ACGAAACCCT CTGTTCAGCT 
GACAGCTTCT GTGTCAATGA CTACGCCTGG GGAGGCTCAC GATGCCATTG CAACCTGGGC 
AAAGGAGGCG AGGCCTGCTC AGAAGATATC TTTATTCAGT ATCCACAGTT CTTTGGCCAC 
TCCTATGTAA CCTTTGAGCC TCTGAAGAAC TCTTATCAGG CATTTCAGAT TACTCTGGAA 
TTCAGGGCAG AGGCAGAAGA TGGCTTACTG CTCTACTGTG GGGAGAGTGA ACATGGGAGA 
GGAGACTTCA TGTCCCTGGC TCTCATCCGA CGCTCCCTCC ACTTCAGGTT TAATTGTGGA 
ACTGGGATGG CCATCATCAT AAGTGAGACC AAAATCAAGC TTGGGGCCTG GCACTCGGTG 
ACACTGTATA GAGATGGGCT GAACGGGCTG CTGCAGTTGA ACAATGGCAC CCCAGTAACA 
GGCCAGTCCC AGGGTCAGTA CAGTAAAATT ACCTTCCGGA CACCTCTGTA CCTTGGTGGT 
GCTCCCAGTG CTTACTGGCT GGTCAGAGCG ACGGGGACAA ATCGTGGCTT CCAAGGCTGT 
GTGCAGTCAC TTGCTGTTAA CGGGAAGAAG ATTGACATGA GGCCCTGGCC ACTGGGGAAA 
GCCCTCAACG GAGCTGATGT TGGGGAGTGC AGCAGTGGGA TCTGTGATGA AGCCTCCTGC 
ATCAACGGGG GCACCTGCGC AGCCATCAAG GCCGACTCCT ACATCTGCCT CTGTCCCCTT 
GGGTTCAGAG GTCGACACTG TGAAGATGCA TTTACCCTGA CCATTCCTCA GTTCAGAGAG 
TCGCTGAGGT CCTACGCTGC CACGCCCTGG CCCCTGGAGC CTCAGCACTA TCTTTCTTTC 
ACCGAGTTTG AGATCACATT TCGGCCAGAC TCCGGGGATG GGGTCCTTTT GTACAGCTAC 
GACACAAGCA GCAAGGACTT CCTGTCCATT ATTATGGCTG CAGGCCATGT GGAATTCCGC 
TTCGACTGTG GTTCTGGGAC CGGAGTTCTC AGGAGTGAGG ATACCCTCAC CCTAGGCCAA 
TGGCATGACC TACGCGTGTC TCGCACAGCG AAGAATGGGA TCTTACAGGT GGACAAGCAG 
AAGGTAGTAG AAGGAATGGC AGAGGGAGGC TTCACCCAGA TTAAGTGTAA TACAGACATT 
TTTATTGGCG GAGTCCCAAA TTACGATGAT GTCAAGAAGA ACTCGGGTAT CCTCCATCCA 
TTTAGTGGGA GCATCCAGAA GATCATCCTA AATGACCGGA CCATCCATGT GAGGCACGAC 
TTTACATCTG GTGTGAATGT GGAGAACGCA GCGCACCCCT GTGTGGGGGC CCCCTGTGCC 
CATGGGGGCA GCTGTAGGCC CAGGAAGGAG GGTTATGAGT GTGACTGTCC CTTGGGCTTC 
GAAGGCCTGA ACTGCCAGAA AGAGTGTGGA AACTACTGCC TCAATACGAT TACAGAAGCC 
ATTGAGATCC CACAGTTCAT CGGTCGGAGT TACCTGACCT ATGACAACCC AAATATCCTC 
AAGAGGGTGT CCGGATCAAG GTCAAATGCT TTCATGAGGT TTAAGACAAC CGCCAAGGAC 
GGCCTGTTGC TGTGGAGGGG GGACAGCCCC ATGAGACCTA ACAGCGACTT CATTTCCCTG 
GGTCTTCGGG ATGGAGCCCT GGTGTTCAGC TACAACCTGG GCAGTGGTGT GGCTTCCATT 
ATGGTGAATG GTTCCTTCAG CGATGGCCGG TGGCACCGAG TCAAGGCTGT CAGGGATGGC 
CAGTCTGGGA AAATTACGGT GGATGACTAT GGAGCCAGAA CAGGCAAGTC ACCTGGCATG 
ATGAGGCAAC TTAACATCAA TGGGGCTCTG TATGTGGGTG GAATGAAGGA AATTGCTCTG 
CACACGAACA GACAGTACAT GCGAGGCCTT GTGGGCTGCA TCTCCCATTT CACATTGTCC 
ACCGATTACC ACATTTCCCT TGTGGAAGAC GCTGTGGATG GGAAGAACAT CAACACTTGT 
GGGGCTAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017446476.1
CDS	1..2592
Translation	MSLWFLILKWPYLGDRERMKEVPQFSPTLWNSLDFDKSWTIIQERLQMDSMVIKGLDPDTNYQFAVRAMNAYGFSLRSQPSNTIRTLGPGEAGSGRYGPGYITDTGVSEDDDASEDELDLDVSFEEVKPLPATKVGNKKSKKTSVSNSEMDSRLAQPTSASLPETTVAVPPTPAQRKGKNSVAVMSRLFDMSCDETLCSADSFCVNDYAWGGSRCHCNLGKGGEACSEDIFIQYPQFFGHSYVTFEPLKNSYQAFQITLEFRAEAEDGLLLYCGESEHGRGDFMSLALIRRSLHFRFNCGTGMAIIISETKIKLGAWHSVTLYRDGLNGLLQLNNGTPVTGQSQGQYSKITFRTPLYLGGAPSAYWLVRATGTNRGFQGCVQSLAVNGKKIDMRPWPLGKALNGADVGECSSGICDEASCINGGTCAAIKADSYICLCPLGFRGRHCEDAFTLTIPQFRESLRSYAATPWPLEPQHYLSFTEFEITFRPDSGDGVLLYSYDTSSKDFLSIIMAAGHVEFRFDCGSGTGVLRSEDTLTLGQWHDLRVSRTAKNGILQVDKQKVVEGMAEGGFTQIKCNTDIFIGGVPNYDDVKKNSGILHPFSGSIQKIILNDRTIHVRHDFTSGVNVENAAHPCVGAPCAHGGSCRPRKEGYECDCPLGFEGLNCQKECGNYCLNTITEAIEIPQFIGRSYLTYDNPNILKRVSGSRSNAFMRFKTTAKDGLLLWRGDSPMRPNSDFISLGLRDGALVFSYNLGSGVASIMVNGSFSDGRWHRVKAVRDGQSGKITVDDYGARTGKSPGMMRQLNINGALYVGGMKEIALHTNRQYMRGLVGCISHFTLSTDYHISLVEDAVDGKNINTCGAK

Target ORF information:

RefSeq Version	XM_017590987.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus EGF-like, fibronectin type III and laminin G domains (Egflam), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017590987.1

ORF Insert Sequence:

ATGTCCTTGT GGTTTCTGAT TCTGAAGTGG CCCTATCTTG GAGACCGGGA GAGAATGAAG 
GAAGTGCCCC AATTCAGTCC TACTCTGTGG AATTCATTAG ATTTTGACAA GAGCTGGACC 
ATAATCCAAG AGCGCCTGCA GATGGACTCC ATGGTTATCA AAGGCCTTGA TCCAGACACC 
AACTACCAGT TTGCTGTGAG AGCCATGAAT GCCTATGGCT TCAGTCTGCG CAGCCAGCCC 
AGCAACACCA TCCGAACTCT GGGTCCTGGG GAGGCTGGAA GTGGCCGCTA TGGACCTGGG 
TATATCACCG ATACCGGCGT GAGTGAGGAT GACGATGCAT CAGAAGACGA GCTAGATCTG 
GATGTCTCCT TTGAGGAGGT TAAACCACTT CCTGCTACCA AAGTTGGGAA TAAGAAGTCC 
AAGAAGACTT CTGTCTCCAA CTCAGAGATG GACTCCAGAC TTGCCCAACC TACCTCAGCA 
TCTCTCCCTG AAACTACAGT GGCTGTGCCA CCCACGCCAG CCCAACGGAA GGGGAAGAAC 
AGTGTGGCCG TGATGTCAAG GCTCTTTGAC ATGTCCTGTG ACGAAACCCT CTGTTCAGCT 
GACAGCTTCT GTGTCAATGA CTACGCCTGG GGAGGCTCAC GATGCCATTG CAACCTGGGC 
AAAGGAGGCG AGGCCTGCTC AGAAGATATC TTTATTCAGT ATCCACAGTT CTTTGGCCAC 
TCCTATGTAA CCTTTGAGCC TCTGAAGAAC TCTTATCAGG CATTTCAGAT TACTCTGGAA 
TTCAGGGCAG AGGCAGAAGA TGGCTTACTG CTCTACTGTG GGGAGAGTGA ACATGGGAGA 
GGAGACTTCA TGTCCCTGGC TCTCATCCGA CGCTCCCTCC ACTTCAGGTT TAATTGTGGA 
ACTGGGATGG CCATCATCAT AAGTGAGACC AAAATCAAGC TTGGGGCCTG GCACTCGGTG 
ACACTGTATA GAGATGGGCT GAACGGGCTG CTGCAGTTGA ACAATGGCAC CCCAGTAACA 
GGCCAGTCCC AGGGTCAGTA CAGTAAAATT ACCTTCCGGA CACCTCTGTA CCTTGGTGGT 
GCTCCCAGTG CTTACTGGCT GGTCAGAGCG ACGGGGACAA ATCGTGGCTT CCAAGGCTGT 
GTGCAGTCAC TTGCTGTTAA CGGGAAGAAG ATTGACATGA GGCCCTGGCC ACTGGGGAAA 
GCCCTCAACG GAGCTGATGT TGGGGAGTGC AGCAGTGGGA TCTGTGATGA AGCCTCCTGC 
ATCAACGGGG GCACCTGCGC AGCCATCAAG GCCGACTCCT ACATCTGCCT CTGTCCCCTT 
GGGTTCAGAG GTCGACACTG TGAAGATGCA TTTACCCTGA CCATTCCTCA GTTCAGAGAG 
TCGCTGAGGT CCTACGCTGC CACGCCCTGG CCCCTGGAGC CTCAGCACTA TCTTTCTTTC 
ACCGAGTTTG AGATCACATT TCGGCCAGAC TCCGGGGATG GGGTCCTTTT GTACAGCTAC 
GACACAAGCA GCAAGGACTT CCTGTCCATT ATTATGGCTG CAGGCCATGT GGAATTCCGC 
TTCGACTGTG GTTCTGGGAC CGGAGTTCTC AGGAGTGAGG ATACCCTCAC CCTAGGCCAA 
TGGCATGACC TACGCGTGTC TCGCACAGCG AAGAATGGGA TCTTACAGGT GGACAAGCAG 
AAGGTAGTAG AAGGAATGGC AGAGGGAGGC TTCACCCAGA TTAAGTGTAA TACAGACATT 
TTTATTGGCG GAGTCCCAAA TTACGATGAT GTCAAGAAGA ACTCGGGTAT CCTCCATCCA 
TTTAGTGGGA GCATCCAGAA GATCATCCTA AATGACCGGA CCATCCATGT GAGGCACGAC 
TTTACATCTG GTGTGAATGT GGAGAACGCA GCGCACCCCT GTGTGGGGGC CCCCTGTGCC 
CATGGGGGCA GCTGTAGGCC CAGGAAGGAG GGTTATGAGT GTGACTGTCC CTTGGGCTTC 
GAAGGCCTGA ACTGCCAGAA AGAGTGTGGA AACTACTGCC TCAATACGAT TACAGAAGCC 
ATTGAGATCC CACAGTTCAT CGGTCGGAGT TACCTGACCT ATGACAACCC AAATATCCTC 
AAGAGGGTGT CCGGATCAAG GTCAAATGCT TTCATGAGGT TTAAGACAAC CGCCAAGGAC 
GGCCTGTTGC TGTGGAGGGG GGACAGCCCC ATGAGACCTA ACAGCGACTT CATTTCCCTG 
GGTCTTCGGG ATGGAGCCCT GGTGTTCAGC TACAACCTGG GCAGTGGTGT GGCTTCCATT 
ATGGTGAATG GTTCCTTCAG CGATGGCCGG TGGCACCGAG TCAAGGCTGT CAGGGATGGC 
CAGTCTGGGA AAATTACGGT GGATGACTAT GGAGCCAGAA CAGGCAAGTC ACCTGGCATG 
ATGAGGCAAC TTAACATCAA TGGGGCTCTG TATGTGGGTG GAATGAAGGA AATTGCTCTG 
CACACGAACA GACAGTACAT GCGAGGCCTT GTGGGCTGCA TCTCCCATTT CACATTGTCC 
ACCGATTACC ACATTTCCCT TGTGGAAGAC GCTGTGGATG GGAAGAACAT CAACACTTGT 
GGGGCTAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Egflam ( NM_001108938.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001102408.2 Rattus norvegicus EGF-like, fibronectin type III and laminin G domains (Egflam), mRNA.

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CloneID	ORa08691
Clone ID Related Accession (Same CDS sequence)	NM_001108938.2
Accession Version	NM_001108938.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3018bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-19
Organism	Rattus norvegicus(Norway rat)
Product	pikachurin precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC168173.1. On Aug 10, 2008 this sequence version replaced NM_001108938.1. ##Evidence-Data-START## Transcript exon combination :: BC168173.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGATTTAA TCTCAACCTT CTTGCTCCAT TTCTTGCTCC TGGCCTGCAG CCTCCCACCC 
GGCGCCGTGT CACTCCGAAC CGCTCTGCGA AAATCAGGCA AGGTGGGACC TCCTCTTGAC 
ATCAAACTGG GAGCCTTGAA CTGTACAGCC TTCAGCATCC AGTGGAAAAC ACCAAAGCGT 
TCTGGTAGCT CCATTGTTGG GTACACAGTA TTTTACTCTG AGCTTGGTTC AGATAAGTCC 
CTCCGGGAAC AGTCACATAA TGTACCTGTT GGCCAGGATA CCCTAATCAC TGAGGAAGTG 
ATTGGAGATT TGAAGCCAGG CACTGAATAT CGAGTGAGCA TAGCAGCCTA CAGCCAGACT 
GGCAAAGGGA GGCTTAGCTT CCCTCGGCAT GTGACCACTT TGTCTCAAGA TTCCTGCCTG 
CCCCCTGAAG CACCCCATCA GCCACATGTC CTTGTGGTTT CTGATTCTGA AGTGGCCCTA 
TCTTGGAGAC CGGGAGAGAA TGAAGGAAGT GCCCCAATTC AGTCCTACTC TGTGGAATTC 
ATTAGGCCAG ATTTTGACAA GAGCTGGACC ATAATCCAAG AGCGCCTGCA GATGGACTCC 
ATGGTTATCA AAGGCCTTGA TCCAGACACC AACTACCAGT TTGCTGTGAG AGCCATGAAT 
GCCTATGGCT TCAGTCTGCG CAGCCAGCCC AGCAACACCA TCCGAACTCT GGGTCCTGGG 
GAGGCTGGAA GTGGCCGCTA TGGACCTGGG TATATCACCG ATACCGGCGT GAGTGAGGAT 
GACGATGCAT CAGAAGACGA GCTAGATCTG GATGTCTCCT TTGAGGAGGT TAAACCACTT 
CCTGCTACCA AAGTTGGGAA TAAGAAGTCC AAGAAGACTT CTGTCTCCAA CTCAGAGATG 
GACTCCAGAC TTGCCCAACC TACCTCAGCA TCTCTCCCTG AAACTACAGT GGCTGTGCCA 
CCCACGCCAG CCCAACGGAA GGGGAAGAAC AGTGTGGCCG TGATGTCAAG GCTCTTTGAC 
ATGTCCTGTG ACGAAACCCT CTGTTCAGCT GACAGCTTCT GTGTCAATGA CTACGCCTGG 
GGAGGCTCAC GATGCCATTG CAACCTGGGC AAAGGAGGCG AGGCCTGCTC AGAAGATATC 
TTTATTCAGT ATCCACAGTT CTTTGGCCAC TCCTATGTAA CCTTTGAGCC TCTGAAGAAC 
TCTTATCAGG CATTTCAGAT TACTCTGGAA TTCAGGGCAG AGGCAGAAGA TGGCTTACTG 
CTCTACTGTG GGGAGAGTGA ACATGGGAGA GGAGACTTCA TGTCCCTGGC TCTCATCCGA 
CGCTCCCTCC ACTTCAGGTT TAATTGTGGA ACTGGGATGG CCATCATCAT AAGTGAGACC 
AAAATCAAGC TTGGGGCCTG GCACTCGGTG ACACTGTATA GAGATGGGCT GAACGGGCTG 
CTGCAGTTGA ACAATGGCAC CCCAGTAACA GGCCAGTCCC AGGGTCAGTA CAGTAAAATT 
ACCTTCCGGA CACCTCTGTA CCTTGGTGGT GCTCCCAGTG CTTACTGGCT GGTCAGAGCG 
ACGGGGACAA ATCGTGGCTT CCAAGGCTGT GTGCAGTCAC TTGCTGTTAA CGGGAAGAAG 
ATTGACATGA GGCCCTGGCC ACTGGGGAAA GCCCTCAACG GAGCTGATGT TGGGGAGTGC 
AGCAGTGGGA TCTGTGATGA AGCCTCCTGC ATCAACGGGG GCACCTGCGC AGCCATCAAG 
GCCGACTCCT ACATCTGCCT CTGTCCCCTT GGGTTCAGAG GTCGACACTG TGAAGATGCA 
TTTACCCTGA CCATTCCTCA GTTCAGAGAG TCGCTGAGGT CCTACGCTGC CACGCCCTGG 
CCCCTGGAGC CTCAGCACTA TCTTTCTTTC ACCGAGTTTG AGATCACATT TCGGCCAGAC 
TCCGGGGATG GGGTCCTTTT GTACAGCTAC GACACAAGCA GCAAGGACTT CCTGTCCATT 
ATTATGGCTG CAGGCCATGT GGAATTCCGC TTCGACTGTG GTTCTGGGAC CGGAGTTCTC 
AGGAGTGAGG ATACCCTCAC CCTAGGCCAA TGGCATGACC TACGCGTGTC TCGCACAGCG 
AAGAATGGGA TCTTACAGGT GGACAAGCAG AAGGTAGTAG AAGGAATGGC AGAGGGAGGC 
TTCACCCAGA TTAAGTGTAA TACAGACATT TTTATTGGCG GAGTCCCAAA TTACGATGAT 
GTCAAGAAGA ACTCGGGTAT CCTCCATCCA TTTAGTGGGA GCATCCAGAA GATCATCCTA 
AATGACCGGA CCATCCATGT GAGGCACGAC TTTACATCTG GTGTGAATGT GGAGAACGCA 
GCGCACCCCT GTGTGGGGGC CCCCTGTGCC CATGGGGGCA GCTGTAGGCC CAGGAAGGAG 
GGTTATGAGT GTGACTGTCC CTTGGGCTTC GAAGGCCTGA ACTGCCAGAA AGCGATTACA 
GAAGCCATTG AGATCCCACA GTTCATCGGT CGGAGTTACC TGACCTATGA CAACCCAAAT 
ATCCTCAAGA GGGTGTCCGG ATCAAGGTCA AATGCTTTCA TGAGGTTTAA GACAACCGCC 
AAGGACGGCC TGTTGCTGTG GAGGGGGGAC AGCCCCATGA GACCTAACAG CGACTTCATT 
TCCCTGGGTC TTCGGGATGG AGCCCTGGTG TTCAGCTACA ACCTGGGCAG TGGTGTGGCT 
TCCATTATGG TGAATGGTTC CTTCAGCGAT GGCCGGTGGC ACCGAGTCAA GGCTGTCAGG 
GATGGCCAGT CTGGGAAAAT TACGGTGGAT GACTATGGAG CCAGAACAGG CAAGTCACCT 
GGCATGATGA GGCAACTTAA CATCAATGGG GCTCTGTATG TGGGTGGAAT GAAGGAAATT 
GCTCTGCGCA CGAACAGACA GTACATGCGA GGCCTTGTGG GCTGCATCTC CCATTTCACA 
TTGTCCACCG ATTACCACAT TTCCCTTGTG GAAGACGCTG TGGATGGGAA GAACATCAAC 
ACTTGTGGGG CTAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001102408.2
CDS	203..3220
Translation	MDLISTFLLHFLLLACSLPPGAVSLRTALRKSGKVGPPLDIKLGALNCTAFSIQWKTPKRSGSSIVGYTVFYSELGSDKSLREQSHNVPVGQDTLITEEVIGDLKPGTEYRVSIAAYSQTGKGRLSFPRHVTTLSQDSCLPPEAPHQPHVLVVSDSEVALSWRPGENEGSAPIQSYSVEFIRPDFDKSWTIIQERLQMDSMVIKGLDPDTNYQFAVRAMNAYGFSLRSQPSNTIRTLGPGEAGSGRYGPGYITDTGVSEDDDASEDELDLDVSFEEVKPLPATKVGNKKSKKTSVSNSEMDSRLAQPTSASLPETTVAVPPTPAQRKGKNSVAVMSRLFDMSCDETLCSADSFCVNDYAWGGSRCHCNLGKGGEACSEDIFIQYPQFFGHSYVTFEPLKNSYQAFQITLEFRAEAEDGLLLYCGESEHGRGDFMSLALIRRSLHFRFNCGTGMAIIISETKIKLGAWHSVTLYRDGLNGLLQLNNGTPVTGQSQGQYSKITFRTPLYLGGAPSAYWLVRATGTNRGFQGCVQSLAVNGKKIDMRPWPLGKALNGADVGECSSGICDEASCINGGTCAAIKADSYICLCPLGFRGRHCEDAFTLTIPQFRESLRSYAATPWPLEPQHYLSFTEFEITFRPDSGDGVLLYSYDTSSKDFLSIIMAAGHVEFRFDCGSGTGVLRSEDTLTLGQWHDLRVSRTAKNGILQVDKQKVVEGMAEGGFTQIKCNTDIFIGGVPNYDDVKKNSGILHPFSGSIQKIILNDRTIHVRHDFTSGVNVENAAHPCVGAPCAHGGSCRPRKEGYECDCPLGFEGLNCQKAITEAIEIPQFIGRSYLTYDNPNILKRVSGSRSNAFMRFKTTAKDGLLLWRGDSPMRPNSDFISLGLRDGALVFSYNLGSGVASIMVNGSFSDGRWHRVKAVRDGQSGKITVDDYGARTGKSPGMMRQLNINGALYVGGMKEIALRTNRQYMRGLVGCISHFTLSTDYHISLVEDAVDGKNINTCGAK

Target ORF information:

RefSeq Version	NM_001108938.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus EGF-like, fibronectin type III and laminin G domains (Egflam), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001108938.2

ORF Insert Sequence:

ATGGATTTAA TCTCAACCTT CTTGCTCCAT TTCTTGCTCC TGGCCTGCAG CCTCCCACCC 
GGCGCCGTGT CACTCCGAAC CGCTCTGCGA AAATCAGGCA AGGTGGGACC TCCTCTTGAC 
ATCAAACTGG GAGCCTTGAA CTGTACAGCC TTCAGCATCC AGTGGAAAAC ACCAAAGCGT 
TCTGGTAGCT CCATTGTTGG GTACACAGTA TTTTACTCTG AGCTTGGTTC AGATAAGTCC 
CTCCGGGAAC AGTCACATAA TGTACCTGTT GGCCAGGATA CCCTAATCAC TGAGGAAGTG 
ATTGGAGATT TGAAGCCAGG CACTGAATAT CGAGTGAGCA TAGCAGCCTA CAGCCAGACT 
GGCAAAGGGA GGCTTAGCTT CCCTCGGCAT GTGACCACTT TGTCTCAAGA TTCCTGCCTG 
CCCCCTGAAG CACCCCATCA GCCACATGTC CTTGTGGTTT CTGATTCTGA AGTGGCCCTA 
TCTTGGAGAC CGGGAGAGAA TGAAGGAAGT GCCCCAATTC AGTCCTACTC TGTGGAATTC 
ATTAGGCCAG ATTTTGACAA GAGCTGGACC ATAATCCAAG AGCGCCTGCA GATGGACTCC 
ATGGTTATCA AAGGCCTTGA TCCAGACACC AACTACCAGT TTGCTGTGAG AGCCATGAAT 
GCCTATGGCT TCAGTCTGCG CAGCCAGCCC AGCAACACCA TCCGAACTCT GGGTCCTGGG 
GAGGCTGGAA GTGGCCGCTA TGGACCTGGG TATATCACCG ATACCGGCGT GAGTGAGGAT 
GACGATGCAT CAGAAGACGA GCTAGATCTG GATGTCTCCT TTGAGGAGGT TAAACCACTT 
CCTGCTACCA AAGTTGGGAA TAAGAAGTCC AAGAAGACTT CTGTCTCCAA CTCAGAGATG 
GACTCCAGAC TTGCCCAACC TACCTCAGCA TCTCTCCCTG AAACTACAGT GGCTGTGCCA 
CCCACGCCAG CCCAACGGAA GGGGAAGAAC AGTGTGGCCG TGATGTCAAG GCTCTTTGAC 
ATGTCCTGTG ACGAAACCCT CTGTTCAGCT GACAGCTTCT GTGTCAATGA CTACGCCTGG 
GGAGGCTCAC GATGCCATTG CAACCTGGGC AAAGGAGGCG AGGCCTGCTC AGAAGATATC 
TTTATTCAGT ATCCACAGTT CTTTGGCCAC TCCTATGTAA CCTTTGAGCC TCTGAAGAAC 
TCTTATCAGG CATTTCAGAT TACTCTGGAA TTCAGGGCAG AGGCAGAAGA TGGCTTACTG 
CTCTACTGTG GGGAGAGTGA ACATGGGAGA GGAGACTTCA TGTCCCTGGC TCTCATCCGA 
CGCTCCCTCC ACTTCAGGTT TAATTGTGGA ACTGGGATGG CCATCATCAT AAGTGAGACC 
AAAATCAAGC TTGGGGCCTG GCACTCGGTG ACACTGTATA GAGATGGGCT GAACGGGCTG 
CTGCAGTTGA ACAATGGCAC CCCAGTAACA GGCCAGTCCC AGGGTCAGTA CAGTAAAATT 
ACCTTCCGGA CACCTCTGTA CCTTGGTGGT GCTCCCAGTG CTTACTGGCT GGTCAGAGCG 
ACGGGGACAA ATCGTGGCTT CCAAGGCTGT GTGCAGTCAC TTGCTGTTAA CGGGAAGAAG 
ATTGACATGA GGCCCTGGCC ACTGGGGAAA GCCCTCAACG GAGCTGATGT TGGGGAGTGC 
AGCAGTGGGA TCTGTGATGA AGCCTCCTGC ATCAACGGGG GCACCTGCGC AGCCATCAAG 
GCCGACTCCT ACATCTGCCT CTGTCCCCTT GGGTTCAGAG GTCGACACTG TGAAGATGCA 
TTTACCCTGA CCATTCCTCA GTTCAGAGAG TCGCTGAGGT CCTACGCTGC CACGCCCTGG 
CCCCTGGAGC CTCAGCACTA TCTTTCTTTC ACCGAGTTTG AGATCACATT TCGGCCAGAC 
TCCGGGGATG GGGTCCTTTT GTACAGCTAC GACACAAGCA GCAAGGACTT CCTGTCCATT 
ATTATGGCTG CAGGCCATGT GGAATTCCGC TTCGACTGTG GTTCTGGGAC CGGAGTTCTC 
AGGAGTGAGG ATACCCTCAC CCTAGGCCAA TGGCATGACC TACGCGTGTC TCGCACAGCG 
AAGAATGGGA TCTTACAGGT GGACAAGCAG AAGGTAGTAG AAGGAATGGC AGAGGGAGGC 
TTCACCCAGA TTAAGTGTAA TACAGACATT TTTATTGGCG GAGTCCCAAA TTACGATGAT 
GTCAAGAAGA ACTCGGGTAT CCTCCATCCA TTTAGTGGGA GCATCCAGAA GATCATCCTA 
AATGACCGGA CCATCCATGT GAGGCACGAC TTTACATCTG GTGTGAATGT GGAGAACGCA 
GCGCACCCCT GTGTGGGGGC CCCCTGTGCC CATGGGGGCA GCTGTAGGCC CAGGAAGGAG 
GGTTATGAGT GTGACTGTCC CTTGGGCTTC GAAGGCCTGA ACTGCCAGAA AGCGATTACA 
GAAGCCATTG AGATCCCACA GTTCATCGGT CGGAGTTACC TGACCTATGA CAACCCAAAT 
ATCCTCAAGA GGGTGTCCGG ATCAAGGTCA AATGCTTTCA TGAGGTTTAA GACAACCGCC 
AAGGACGGCC TGTTGCTGTG GAGGGGGGAC AGCCCCATGA GACCTAACAG CGACTTCATT 
TCCCTGGGTC TTCGGGATGG AGCCCTGGTG TTCAGCTACA ACCTGGGCAG TGGTGTGGCT 
TCCATTATGG TGAATGGTTC CTTCAGCGAT GGCCGGTGGC ACCGAGTCAA GGCTGTCAGG 
GATGGCCAGT CTGGGAAAAT TACGGTGGAT GACTATGGAG CCAGAACAGG CAAGTCACCT 
GGCATGATGA GGCAACTTAA CATCAATGGG GCTCTGTATG TGGGTGGAAT GAAGGAAATT 
GCTCTGCGCA CGAACAGACA GTACATGCGA GGCCTTGTGG GCTGCATCTC CCATTTCACA 
TTGTCCACCG ATTACCACAT TTCCCTTGTG GAAGACGCTG TGGATGGGAA GAACATCAAC 
ACTTGTGGGG CTAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Egflam ( XM_006232013.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006232075.1 Rattus norvegicus EGF-like, fibronectin type III and laminin G domains (Egflam), transcript variant X1, mRNA.

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CloneID	ORa24451
Clone ID Related Accession (Same CDS sequence)	XM_006232013.3
Accession Version	XM_006232013.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3042bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	pikachurin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006232013.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTTAA TCTCAACCTT CTTGCTCCAT TTCTTGCTCC TGGCCTGCAG CCTCCCACCC 
GGCGCCGTGT CACTCCGAAC CGCTCTGCGA AAATCAGGCA AGGTGGGACC TCCTCTTGAC 
ATCAAACTGG GAGCCTTGAA CTGTACAGCC TTCAGCATCC AGTGGAAAAC ACCAAAGCGT 
TCTGGTAGCT CCATTGTTGG GTACACAGTA TTTTACTCTG AGCTTGGTTC AGATAAGTCC 
CTCCGGGAAC AGTCACATAA TGTACCTGTT GGCCAGGATA CCCTAATCAC TGAGGAAGTG 
ATTGGAGATT TGAAGCCAGG CACTGAATAT CGAGTGAGCA TAGCAGCCTA CAGCCAGACT 
GGCAAAGGGA GGCTTAGCTT CCCTCGGCAT GTGACCACTT TGTCTCAAGA TTCCTGCCTG 
CCCCCTGAAG CACCCCATCA GCCACATGTC CTTGTGGTTT CTGATTCTGA AGTGGCCCTA 
TCTTGGAGAC CGGGAGAGAA TGAAGGAAGT GCCCCAATTC AGTCCTACTC TGTGGAATTC 
ATTAGGCCAG ATTTTGACAA GAGCTGGACC ATAATCCAAG AGCGCCTGCA GATGGACTCC 
ATGGTTATCA AAGGCCTTGA TCCAGACACC AACTACCAGT TTGCTGTGAG AGCCATGAAT 
GCCTATGGCT TCAGTCTGCG CAGCCAGCCC AGCAACACCA TCCGAACTCT GGGTCCTGGG 
GAGGCTGGAA GTGGCCGCTA TGGACCTGGG TATATCACCG ATACCGGCGT GAGTGAGGAT 
GACGATGCAT CAGAAGACGA GCTAGATCTG GATGTCTCCT TTGAGGAGGT TAAACCACTT 
CCTGCTACCA AAGTTGGGAA TAAGAAGTCC AAGAAGACTT CTGTCTCCAA CTCAGAGATG 
GACTCCAGAC TTGCCCAACC TACCTCAGCA TCTCTCCCTG AAACTACAGT GGCTGTGCCA 
CCCACGCCAG CCCAACGGAA GGGGAAGAAC AGTGTGGCCG TGATGTCAAG GCTCTTTGAC 
ATGTCCTGTG ACGAAACCCT CTGTTCAGCT GACAGCTTCT GTGTCAATGA CTACGCCTGG 
GGAGGCTCAC GATGCCATTG CAACCTGGGC AAAGGAGGCG AGGCCTGCTC AGAAGATATC 
TTTATTCAGT ATCCACAGTT CTTTGGCCAC TCCTATGTAA CCTTTGAGCC TCTGAAGAAC 
TCTTATCAGG CATTTCAGAT TACTCTGGAA TTCAGGGCAG AGGCAGAAGA TGGCTTACTG 
CTCTACTGTG GGGAGAGTGA ACATGGGAGA GGAGACTTCA TGTCCCTGGC TCTCATCCGA 
CGCTCCCTCC ACTTCAGGTT TAATTGTGGA ACTGGGATGG CCATCATCAT AAGTGAGACC 
AAAATCAAGC TTGGGGCCTG GCACTCGGTG ACACTGTATA GAGATGGGCT GAACGGGCTG 
CTGCAGTTGA ACAATGGCAC CCCAGTAACA GGCCAGTCCC AGGGTCAGTA CAGTAAAATT 
ACCTTCCGGA CACCTCTGTA CCTTGGTGGT GCTCCCAGTG CTTACTGGCT GGTCAGAGCG 
ACGGGGACAA ATCGTGGCTT CCAAGGCTGT GTGCAGTCAC TTGCTGTTAA CGGGAAGAAG 
ATTGACATGA GGCCCTGGCC ACTGGGGAAA GCCCTCAACG GAGCTGATGT TGGGGAGTGC 
AGCAGTGGGA TCTGTGATGA AGCCTCCTGC ATCAACGGGG GCACCTGCGC AGCCATCAAG 
GCCGACTCCT ACATCTGCCT CTGTCCCCTT GGGTTCAGAG GTCGACACTG TGAAGATGCA 
TTTACCCTGA CCATTCCTCA GTTCAGAGAG TCGCTGAGGT CCTACGCTGC CACGCCCTGG 
CCCCTGGAGC CTCAGCACTA TCTTTCTTTC ACCGAGTTTG AGATCACATT TCGGCCAGAC 
TCCGGGGATG GGGTCCTTTT GTACAGCTAC GACACAAGCA GCAAGGACTT CCTGTCCATT 
ATTATGGCTG CAGGCCATGT GGAATTCCGC TTCGACTGTG GTTCTGGGAC CGGAGTTCTC 
AGGAGTGAGG ATACCCTCAC CCTAGGCCAA TGGCATGACC TACGCGTGTC TCGCACAGCG 
AAGAATGGGA TCTTACAGGT GGACAAGCAG AAGGTAGTAG AAGGAATGGC AGAGGGAGGC 
TTCACCCAGA TTAAGTGTAA TACAGACATT TTTATTGGCG GAGTCCCAAA TTACGATGAT 
GTCAAGAAGA ACTCGGGTAT CCTCCATCCA TTTAGTGGGA GCATCCAGAA GATCATCCTA 
AATGACCGGA CCATCCATGT GAGGCACGAC TTTACATCTG GTGTGAATGT GGAGAACGCA 
GCGCACCCCT GTGTGGGGGC CCCCTGTGCC CATGGGGGCA GCTGTAGGCC CAGGAAGGAG 
GGTTATGAGT GTGACTGTCC CTTGGGCTTC GAAGGCCTGA ACTGCCAGAA AGAGTGTGGA 
AACTACTGCC TCAATACGAT TACAGAAGCC ATTGAGATCC CACAGTTCAT CGGTCGGAGT 
TACCTGACCT ATGACAACCC AAATATCCTC AAGAGGGTGT CCGGATCAAG GTCAAATGCT 
TTCATGAGGT TTAAGACAAC CGCCAAGGAC GGCCTGTTGC TGTGGAGGGG GGACAGCCCC 
ATGAGACCTA ACAGCGACTT CATTTCCCTG GGTCTTCGGG ATGGAGCCCT GGTGTTCAGC 
TACAACCTGG GCAGTGGTGT GGCTTCCATT ATGGTGAATG GTTCCTTCAG CGATGGCCGG 
TGGCACCGAG TCAAGGCTGT CAGGGATGGC CAGTCTGGGA AAATTACGGT GGATGACTAT 
GGAGCCAGAA CAGGCAAGTC ACCTGGCATG ATGAGGCAAC TTAACATCAA TGGGGCTCTG 
TATGTGGGTG GAATGAAGGA AATTGCTCTG CACACGAACA GACAGTACAT GCGAGGCCTT 
GTGGGCTGCA TCTCCCATTT CACATTGTCC ACCGATTACC ACATTTCCCT TGTGGAAGAC 
GCTGTGGATG GGAAGAACAT CAACACTTGT GGGGCTAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006232075.1
CDS	1595..4636
Translation	MDLISTFLLHFLLLACSLPPGAVSLRTALRKSGKVGPPLDIKLGALNCTAFSIQWKTPKRSGSSIVGYTVFYSELGSDKSLREQSHNVPVGQDTLITEEVIGDLKPGTEYRVSIAAYSQTGKGRLSFPRHVTTLSQDSCLPPEAPHQPHVLVVSDSEVALSWRPGENEGSAPIQSYSVEFIRPDFDKSWTIIQERLQMDSMVIKGLDPDTNYQFAVRAMNAYGFSLRSQPSNTIRTLGPGEAGSGRYGPGYITDTGVSEDDDASEDELDLDVSFEEVKPLPATKVGNKKSKKTSVSNSEMDSRLAQPTSASLPETTVAVPPTPAQRKGKNSVAVMSRLFDMSCDETLCSADSFCVNDYAWGGSRCHCNLGKGGEACSEDIFIQYPQFFGHSYVTFEPLKNSYQAFQITLEFRAEAEDGLLLYCGESEHGRGDFMSLALIRRSLHFRFNCGTGMAIIISETKIKLGAWHSVTLYRDGLNGLLQLNNGTPVTGQSQGQYSKITFRTPLYLGGAPSAYWLVRATGTNRGFQGCVQSLAVNGKKIDMRPWPLGKALNGADVGECSSGICDEASCINGGTCAAIKADSYICLCPLGFRGRHCEDAFTLTIPQFRESLRSYAATPWPLEPQHYLSFTEFEITFRPDSGDGVLLYSYDTSSKDFLSIIMAAGHVEFRFDCGSGTGVLRSEDTLTLGQWHDLRVSRTAKNGILQVDKQKVVEGMAEGGFTQIKCNTDIFIGGVPNYDDVKKNSGILHPFSGSIQKIILNDRTIHVRHDFTSGVNVENAAHPCVGAPCAHGGSCRPRKEGYECDCPLGFEGLNCQKECGNYCLNTITEAIEIPQFIGRSYLTYDNPNILKRVSGSRSNAFMRFKTTAKDGLLLWRGDSPMRPNSDFISLGLRDGALVFSYNLGSGVASIMVNGSFSDGRWHRVKAVRDGQSGKITVDDYGARTGKSPGMMRQLNINGALYVGGMKEIALHTNRQYMRGLVGCISHFTLSTDYHISLVEDAVDGKNINTCGAK

Target ORF information:

RefSeq Version	XM_006232013.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus EGF-like, fibronectin type III and laminin G domains (Egflam), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006232013.3

ORF Insert Sequence:

ATGGATTTAA TCTCAACCTT CTTGCTCCAT TTCTTGCTCC TGGCCTGCAG CCTCCCACCC 
GGCGCCGTGT CACTCCGAAC CGCTCTGCGA AAATCAGGCA AGGTGGGACC TCCTCTTGAC 
ATCAAACTGG GAGCCTTGAA CTGTACAGCC TTCAGCATCC AGTGGAAAAC ACCAAAGCGT 
TCTGGTAGCT CCATTGTTGG GTACACAGTA TTTTACTCTG AGCTTGGTTC AGATAAGTCC 
CTCCGGGAAC AGTCACATAA TGTACCTGTT GGCCAGGATA CCCTAATCAC TGAGGAAGTG 
ATTGGAGATT TGAAGCCAGG CACTGAATAT CGAGTGAGCA TAGCAGCCTA CAGCCAGACT 
GGCAAAGGGA GGCTTAGCTT CCCTCGGCAT GTGACCACTT TGTCTCAAGA TTCCTGCCTG 
CCCCCTGAAG CACCCCATCA GCCACATGTC CTTGTGGTTT CTGATTCTGA AGTGGCCCTA 
TCTTGGAGAC CGGGAGAGAA TGAAGGAAGT GCCCCAATTC AGTCCTACTC TGTGGAATTC 
ATTAGGCCAG ATTTTGACAA GAGCTGGACC ATAATCCAAG AGCGCCTGCA GATGGACTCC 
ATGGTTATCA AAGGCCTTGA TCCAGACACC AACTACCAGT TTGCTGTGAG AGCCATGAAT 
GCCTATGGCT TCAGTCTGCG CAGCCAGCCC AGCAACACCA TCCGAACTCT GGGTCCTGGG 
GAGGCTGGAA GTGGCCGCTA TGGACCTGGG TATATCACCG ATACCGGCGT GAGTGAGGAT 
GACGATGCAT CAGAAGACGA GCTAGATCTG GATGTCTCCT TTGAGGAGGT TAAACCACTT 
CCTGCTACCA AAGTTGGGAA TAAGAAGTCC AAGAAGACTT CTGTCTCCAA CTCAGAGATG 
GACTCCAGAC TTGCCCAACC TACCTCAGCA TCTCTCCCTG AAACTACAGT GGCTGTGCCA 
CCCACGCCAG CCCAACGGAA GGGGAAGAAC AGTGTGGCCG TGATGTCAAG GCTCTTTGAC 
ATGTCCTGTG ACGAAACCCT CTGTTCAGCT GACAGCTTCT GTGTCAATGA CTACGCCTGG 
GGAGGCTCAC GATGCCATTG CAACCTGGGC AAAGGAGGCG AGGCCTGCTC AGAAGATATC 
TTTATTCAGT ATCCACAGTT CTTTGGCCAC TCCTATGTAA CCTTTGAGCC TCTGAAGAAC 
TCTTATCAGG CATTTCAGAT TACTCTGGAA TTCAGGGCAG AGGCAGAAGA TGGCTTACTG 
CTCTACTGTG GGGAGAGTGA ACATGGGAGA GGAGACTTCA TGTCCCTGGC TCTCATCCGA 
CGCTCCCTCC ACTTCAGGTT TAATTGTGGA ACTGGGATGG CCATCATCAT AAGTGAGACC 
AAAATCAAGC TTGGGGCCTG GCACTCGGTG ACACTGTATA GAGATGGGCT GAACGGGCTG 
CTGCAGTTGA ACAATGGCAC CCCAGTAACA GGCCAGTCCC AGGGTCAGTA CAGTAAAATT 
ACCTTCCGGA CACCTCTGTA CCTTGGTGGT GCTCCCAGTG CTTACTGGCT GGTCAGAGCG 
ACGGGGACAA ATCGTGGCTT CCAAGGCTGT GTGCAGTCAC TTGCTGTTAA CGGGAAGAAG 
ATTGACATGA GGCCCTGGCC ACTGGGGAAA GCCCTCAACG GAGCTGATGT TGGGGAGTGC 
AGCAGTGGGA TCTGTGATGA AGCCTCCTGC ATCAACGGGG GCACCTGCGC AGCCATCAAG 
GCCGACTCCT ACATCTGCCT CTGTCCCCTT GGGTTCAGAG GTCGACACTG TGAAGATGCA 
TTTACCCTGA CCATTCCTCA GTTCAGAGAG TCGCTGAGGT CCTACGCTGC CACGCCCTGG 
CCCCTGGAGC CTCAGCACTA TCTTTCTTTC ACCGAGTTTG AGATCACATT TCGGCCAGAC 
TCCGGGGATG GGGTCCTTTT GTACAGCTAC GACACAAGCA GCAAGGACTT CCTGTCCATT 
ATTATGGCTG CAGGCCATGT GGAATTCCGC TTCGACTGTG GTTCTGGGAC CGGAGTTCTC 
AGGAGTGAGG ATACCCTCAC CCTAGGCCAA TGGCATGACC TACGCGTGTC TCGCACAGCG 
AAGAATGGGA TCTTACAGGT GGACAAGCAG AAGGTAGTAG AAGGAATGGC AGAGGGAGGC 
TTCACCCAGA TTAAGTGTAA TACAGACATT TTTATTGGCG GAGTCCCAAA TTACGATGAT 
GTCAAGAAGA ACTCGGGTAT CCTCCATCCA TTTAGTGGGA GCATCCAGAA GATCATCCTA 
AATGACCGGA CCATCCATGT GAGGCACGAC TTTACATCTG GTGTGAATGT GGAGAACGCA 
GCGCACCCCT GTGTGGGGGC CCCCTGTGCC CATGGGGGCA GCTGTAGGCC CAGGAAGGAG 
GGTTATGAGT GTGACTGTCC CTTGGGCTTC GAAGGCCTGA ACTGCCAGAA AGAGTGTGGA 
AACTACTGCC TCAATACGAT TACAGAAGCC ATTGAGATCC CACAGTTCAT CGGTCGGAGT 
TACCTGACCT ATGACAACCC AAATATCCTC AAGAGGGTGT CCGGATCAAG GTCAAATGCT 
TTCATGAGGT TTAAGACAAC CGCCAAGGAC GGCCTGTTGC TGTGGAGGGG GGACAGCCCC 
ATGAGACCTA ACAGCGACTT CATTTCCCTG GGTCTTCGGG ATGGAGCCCT GGTGTTCAGC 
TACAACCTGG GCAGTGGTGT GGCTTCCATT ATGGTGAATG GTTCCTTCAG CGATGGCCGG 
TGGCACCGAG TCAAGGCTGT CAGGGATGGC CAGTCTGGGA AAATTACGGT GGATGACTAT 
GGAGCCAGAA CAGGCAAGTC ACCTGGCATG ATGAGGCAAC TTAACATCAA TGGGGCTCTG 
TATGTGGGTG GAATGAAGGA AATTGCTCTG CACACGAACA GACAGTACAT GCGAGGCCTT 
GTGGGCTGCA TCTCCCATTT CACATTGTCC ACCGATTACC ACATTTCCCT TGTGGAAGAC 
GCTGTGGATG GGAAGAACAT CAACACTTGT GGGGCTAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cell specific post-translational processing of pikachurin, a protein involved in retinal synaptogenesis.
PloS one7(12)e50552(2012)
Han J,Townes-Anderson E

Transcriptome-based systematic identification of extracellular matrix proteins.
Proceedings of the National Academy of Sciences of the United States of America105(35)12849-54(2008 Sep)
Manabe R,Tsutsui K,Yamada T,Kimura M,Nakano I,Shimono C,Sanzen N,Furutani Y,Fukuda T,Oguri Y,Shimamoto K,Kiyozumi D,Sato Y,Sado Y,Senoo H,Yamashina S,Fukuda S,Kawai J,Sugiura N,Kimata K,Hayashizaki Y,Sekiguchi K

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Egflam cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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