Col4a5 cDNA ORF clone, Rattus norvegicus(Norway rat)

The following Col4a5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Col4a5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ORa21617 XM_343778.6
Latest version!
Rattus norvegicus collagen type IV alpha 5 chain (Col4a5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
ORa21617 XM_001055156.4
Latest version!
Rattus norvegicus collagen type IV alpha 5 chain (Col4a5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
ORa21618 XM_006257312.1
Latest version!
Rattus norvegicus collagen type IV alpha 5 chain (Col4a5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
ORa21618 XM_006227490.1
Latest version!
Rattus norvegicus collagen type IV alpha 5 chain (Col4a5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
ORa21619 XM_006227491.1
Latest version!
Rattus norvegicus collagen type IV alpha 5 chain (Col4a5), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
ORa21619 XM_006257313.1
Latest version!
Rattus norvegicus collagen type IV alpha 5 chain (Col4a5), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
ORa48575 XM_008758534.1
Latest version!
Rattus norvegicus collagen type IV alpha 5 chain (Col4a5), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
ORa48575 XM_008773475.1
Latest version!
Rattus norvegicus collagen type IV alpha 5 chain (Col4a5), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ORa21617
    Clone ID Related Accession (Same CDS sequence) XM_343778.6 , XM_001055156.4
    Accession Version XM_343778.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5076bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product collagen alpha-5(IV) chain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 9, 2013 this sequence version replaced XM_343778.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCAAGTGC CTGGAGTGTG CCTGGCCGCC GGCTGGTTCT TACTGGCCCT GTGTCTGTGG 
    GGGCAGCCGG GAGAGGCTGC GGCCTGCCAT GGGTGTTCTT CTGGATCAAA ATGTGACTGC
    AGTGGTATAA AAGGAGAAAA GGGAGAACGG GGCTTTCCAG GCTTAGAAGG CCATCCAGGT
    TTACCAGGAT TTCCAGGTCC AGAAGGGCCT CCAGGACCTC GGGGACAAAA GGGTGATGAT
    GGAATTCCAG GGCCACCAGG ACCAAAAGGA ATCAGAGGTC CTCCTGGACT TCCTGGATTT
    CCAGGGACAC CAGGCCTTCC TGGGATGCCA GGCCACGATG GGGCTCCAGG ACCTCAAGGT
    ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGCGGATTTC CAGGCAGTCC AGGCTTTCCC
    GGTTTACAAG GACCTCCAGG ACCACCTGGG ATCCCAGGTA TGAAGGGGGA GCCAGGCAGT
    ATAATCATGT CATCTCTGCC AGGGCCAAAG GGTAATCAAG GATACCCAGG TCCTCCTGGA
    ATACAAGGTC CACCTGGTCC CAAGGGTGTA TCAGGGCCAA GTGGTCCTCC AGGACCACCA
    GGTTTAATGG GCCCTCCTGG CCCACCAGGC CCACCGGGAC CAAAGGGGAA TATGGGCTTA
    AATTTCCAGG GACCCAAAGG CGAAAAAGGT GAACAAGGTC TTCAGGGTCC ACCTGGTCCA
    CCTGGGCAGA TCAGTGAACA GAAAAGACCA ATTGACCTAG ATTTTCAGAA AGGCGATCAG
    GGAGTTCCCG GTGATAGAGG GCCTCCTGGA CCTCCGGGAA TACCTGGTCC TCCCGGTCCT
    CCTGGTGGTG TGAAAGGTGA AAAGGGTGAG CCCGGAGAGC CAGGCAAAAG AGGTAAACCA
    GGCAAAGATG GAGAGACTGG TCAACCAGGA ATCCCAGGTT TGCCTGGTGA TCCTGGTTAC
    CCTGGTGAAC CAGGAAGGCA TGGTGAAAAG GGCCAAAAGG GTGATACTGG CCCACCTGGA
    CCACCTGGAT TTGTAATTCC TACACCTGGA ACTGGTGTGA CTATTGGAGC AAAAGGAAAT
    ATTGGGCAAC CTGGTTTGCC TGGAGAAAAA GGAGAGCGAG GATTTCCTGG CATACAAGGT
    CCACCTGGCT TTCCTGGACC TCCAGGGACA GCTGTCATGG GTCCCCCTGG TCCTCCTGGA
    TATCCTGGTG AAAGGGGCCA GAAAGGTGAT GAAGGTCCCC CTGGAATTTG TATTCCTGGA
    TCTCCTGGAC TTGATGGACA GCCTGGAGCT CCTGGCTTTC CAGGACCTCC TGGTCCTCCT
    GGCCCCCAGT TACCCCCGAG TGATGAAATC TGTAAAGCAG GCCCTCCAGG GCCCCCAGGA
    TCTCCAGGTG ACCAAGGACT CCAAGGAGAG CAAGGAGTAA AAGGTGACAA AGGTGATACT
    TGCTTCAACT GTATTGGAAC TGGCATTTCA GGGCCTCCAG GCCAACCAGG TTTACCAGGT
    CTCCCAGGTC CTCCAGGATC TCTTGGAATC CCTGGAGAGA AGGGCGAGAA AGGACAAGCT
    GGGACAACTG GTCCAAAAGG ATTGCCAGGC ATTCCTGGAC CTCCAGGTGC TCCAGGCTTT
    CCAGGGTCTA AGGGTGACCC TGGTGATGTT CTCACTCTTC CGGGAATGAA AGGTGAAAAA
    GGAGAATTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTC TACCTGGTAG TCCTGGAAAG
    GATGGTTTGC CAGGACTTCC TGGCCCCAAA GGAGAGCCCG GTGGAATCAC TTTTAAGGGT
    GAAAGGGGTC CTCCTGGGAA CCCAGGTTTA CCAGGTCTCC CAGGAAATAT GGGACCTACA
    GGCCCTGTTG GTTTTGGGCC TCCAGGCCCC GTAGGTGAAA AAGGCATACA AGGTGTGGCG
    GGAAATCCAG GCCAGCCAGG ATTACCAGGT CCTAAAGGTG ATCCAGGCCA GACCATCAGC
    CAGCCAGGAA AGCCTGGCTT GCCAGGGAAT CCAGGCAGAG ATGGTGAAGT GGGTCTTCCA
    GGTGATCCTG GACTTCCAGG CCAACCAGGC CTACCAGGAA TACCTGGTAG CAAAGGAGAA
    CCAGGTATCC CTGGGATTGG GCCTCCAGGA CCACCTGGTC CCAAAGGTTT TCCAGGAATT
    CCTGGACCTC CAGGACCTCC TGGGGCCCCT GGAAGAATGG GCCCAGAAGG GCCTCCTGGG
    TTACCGGGCT TTCCAGGACC CAAGGGAGAG CCAGGATTTG GGTTACCTGG GCCACCTGGG
    CCACCAGGAT TCCCAGGTTT CAAAGGAACA CCTGGTCCAA AAGGTGATCG TGGTTTACCA
    GGACCTCCAG GTCCTCCAGG ACACACTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAT
    GCTGGACCAA ATGGACAACC TGGCCCAATA GGGCCACCAG GGCTGCCAGG AATTGGTCTT
    CAGGGACCAC CAGGACCACC AGGGACTCCA GGGCCAATAG GCCAACCTGG CTTACATGGA
    ATACCAGGAG AGAAGGGAGA TCCAGGACCT CCTGGGCTTG ATGTTCCAGG ACTCCCTGGA
    GAGAGAGGTA GTCCAGGGCT CCCTGGAGCC CCTGGTCTTA TAGGACCTCC AGGATCACCT
    GGTATCCCTG GAAGAGCAGG TGTCCCTGGA TTTCCAGGTG TCAAAGGCGA AATGGGTATG
    ATGGGACCTC CAGGCCCACC GGGACCTTTG GGTACTCCTG GCAGGAGTGG TGCTCCTGGT
    CTTAAAGGTG ATGACGGCAT GCAAGGTCAG CCAGGTCTCC CTGGCCCTGT AGGAGAAAAG
    GGTGGTAAGG GAGAACCTGG CCTTCCAGGT CCTCCTGGAC CAATGCGTCC AGATTTCCTG
    GGATCAAAAG GAGAAAAGGG TGATCCTGGC TTACCAGGTA TTCCTGGACT TTCCGGACCA
    AAAGGCTACC AGGGATTACC TGGAGACCCA GGGCAACCTG GGCTGAGTGG ACAGCCGGGA
    TCACCAGGAC TACCAGGTCC CAAAGGTACT CCTGGTCTCC CTGGTCCACC AGGACTTACC
    GGACCTCCTG GACCTAAAGG AAACATTGGC GACATGGGTT TTCCTGGCCC TCAAGGTGTA
    GATGGGCCTC CTGGACCTCC TGGAGGTCCT GGACAACCTG GCTCTCCCGG GTTGCCTGGC
    CAGAAAGGAA GTAAAGGAGA ACCTGGAGTT TCAGGCATTG GTCTCCCTGG TCTTCCTGGT
    CCAAAGGGTG AACCTGGTCT GCCTGGATAT CCAGGAAACC CAGGTATCAA AGGTTCTATG
    GGCGAGGCTG GTCTGCCTGG CTTACCAGGA AGCCCTGGAG CAAAAGGTCA ACCCGGCCTG
    CCCGGATTCC CAGGAACACC AGGGCTTCCT GGACCAAAGG GTATGAATGG TCCTCCTGGT
    AACCCTGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGGCGTCC TGGACCACCA
    GGACCTCCTG GTGAAAAAGG CAAGCCAGGT CAAGATGGCA TTCCTGGACC AGCAGGACAA
    AAGGGAGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CTCCTGGACT CCCAGGACTT
    TCTGGTCAAA AGGGTGATGG AGGAGCCCCT GGCCTTCCAG GAAATCCTGG CCTTCCAGGT
    TCAAAGGGTG AACCAGGCTT TCAGGGATTC CCTGGTATGC CAGGTCCCCC AGGTCCCCCT
    GGTTCTCCTG GCCCATCTTT GGAAGGTCCC AAAGGAAACC CTGGGCCTCA AGGCCCTCCT
    GGGAGACCAG GTCCTCCAGG TTTTCAAGGT CCACCTGGTA CAGAAGGTCC TCGTGGCCCC
    CCTGGAAATG GAGGTATTAA AGGAGAGAGG GGAAATCCAG GTCCACCTGG GCAACCAGGC
    TTACCTGGCT TGAAAGGAGA TCAAGGACCA CCAGGCCTTC CGGGTAACCC TGGCCGACCA
    GGTCTTAATG GAATGAAAGG AGATCCTGGT CTCCCTGGTG TTCCAGGATT CCCAGGAATG
    AAAGGACCCA TTGGAGTACC AGGCTCAACT GGCCCTGATG GGGAACCAGG TCTTACGGGG
    CCCCCAGGTC CTCCTGGGTT GCCTGGTCCT TCAGGACAGA GTATTGTAAT CAAAGGAGAT
    CCTGGACCTC CAGGAATTCC TGGACAACCT GGATTAAAAG GTCTACCAGG ACTTCCAGGC
    CCTCAAGGTC TACCAGGTCC AATTGGCCCT CCAGGAGATC CAGGACGCAA TGGACTCCCA
    GGCTTTGATG GTGCAGGAGG GCGCAAAGGA GACCCAGGCC TGCCGGGGCA GCCAGGTAGC
    CGTGGATTGG ATGGTCCCCC AGGACCAGAT GGATTGCAAG GACCTCCAGG GCCCCCGGGA
    ACCACCTCTG TTGCCCATGG ATTCCTCATC ACACGTCACA GTCAGACAAC AGATGCACCA
    CAATGCCCAC AGGGAACAGT CCATATCTAT GATGGCTTTT CTCTCTTATA TGTACAAGGA
    AATAAAAGAG CCCATGGTCA AGACTTGGGG ACAGCTGGCA GCTGCCTTCG TCGCTTCAGT
    ACCATGCCTT TCATGTTCTG CAACATTAAC AACGTTTGTA ACTTTGCTTC AAGAAATGAC
    TATTCTTACT GGCTTTCCAC CCCAGAGCCC ATGCCAATGA ACATGGAACC CCTGAAGGGC
    CAGAGCATCC AGCCATTCAT TAGTCGATGT GCAGTATGTG AGGCACCAGC TGTGGTGATT
    GCAGTTCACA GCCAGACTAT TCAGATTCCT CACTGTCCTC AGGGGTGGGA CTCTCTGTGG
    ATTGGCTATT CATTCATGAT GCATACAAGT GCAGGAGCAG AAGGCTCTGG CCAAGCCCTA
    GCCTCTCCTG GCTCCTGTTT GGAAGAATTT CGTTCAGCTC CCTTCATCGA ATGTCATGGA
    CGAGGGACAT GCAATTACTA TGCCAATTCT TACAGCTTTT GGCTGGCCAC TGTTGATATG
    TCAGACATGT TCAGTAAACC TCAGTCAGAG ACACTGAAAG CAGGCGACTT GAGGACACGT
    ATTAGCCGAT GTCAAGTATG CATGAAAAGG ACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_343779.2
    CDS400..5475
    Translation

    Target ORF information:

    RefSeq Version XM_343778.6
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus collagen type IV alpha 5 chain (Col4a5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_343778.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCAAGTGC CTGGAGTGTG CCTGGCCGCC GGCTGGTTCT TACTGGCCCT GTGTCTGTGG 
    GGGCAGCCGG GAGAGGCTGC GGCCTGCCAT GGGTGTTCTT CTGGATCAAA ATGTGACTGC
    AGTGGTATAA AAGGAGAAAA GGGAGAACGG GGCTTTCCAG GCTTAGAAGG CCATCCAGGT
    TTACCAGGAT TTCCAGGTCC AGAAGGGCCT CCAGGACCTC GGGGACAAAA GGGTGATGAT
    GGAATTCCAG GGCCACCAGG ACCAAAAGGA ATCAGAGGTC CTCCTGGACT TCCTGGATTT
    CCAGGGACAC CAGGCCTTCC TGGGATGCCA GGCCACGATG GGGCTCCAGG ACCTCAAGGT
    ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGCGGATTTC CAGGCAGTCC AGGCTTTCCC
    GGTTTACAAG GACCTCCAGG ACCACCTGGG ATCCCAGGTA TGAAGGGGGA GCCAGGCAGT
    ATAATCATGT CATCTCTGCC AGGGCCAAAG GGTAATCAAG GATACCCAGG TCCTCCTGGA
    ATACAAGGTC CACCTGGTCC CAAGGGTGTA TCAGGGCCAA GTGGTCCTCC AGGACCACCA
    GGTTTAATGG GCCCTCCTGG CCCACCAGGC CCACCGGGAC CAAAGGGGAA TATGGGCTTA
    AATTTCCAGG GACCCAAAGG CGAAAAAGGT GAACAAGGTC TTCAGGGTCC ACCTGGTCCA
    CCTGGGCAGA TCAGTGAACA GAAAAGACCA ATTGACCTAG ATTTTCAGAA AGGCGATCAG
    GGAGTTCCCG GTGATAGAGG GCCTCCTGGA CCTCCGGGAA TACCTGGTCC TCCCGGTCCT
    CCTGGTGGTG TGAAAGGTGA AAAGGGTGAG CCCGGAGAGC CAGGCAAAAG AGGTAAACCA
    GGCAAAGATG GAGAGACTGG TCAACCAGGA ATCCCAGGTT TGCCTGGTGA TCCTGGTTAC
    CCTGGTGAAC CAGGAAGGCA TGGTGAAAAG GGCCAAAAGG GTGATACTGG CCCACCTGGA
    CCACCTGGAT TTGTAATTCC TACACCTGGA ACTGGTGTGA CTATTGGAGC AAAAGGAAAT
    ATTGGGCAAC CTGGTTTGCC TGGAGAAAAA GGAGAGCGAG GATTTCCTGG CATACAAGGT
    CCACCTGGCT TTCCTGGACC TCCAGGGACA GCTGTCATGG GTCCCCCTGG TCCTCCTGGA
    TATCCTGGTG AAAGGGGCCA GAAAGGTGAT GAAGGTCCCC CTGGAATTTG TATTCCTGGA
    TCTCCTGGAC TTGATGGACA GCCTGGAGCT CCTGGCTTTC CAGGACCTCC TGGTCCTCCT
    GGCCCCCAGT TACCCCCGAG TGATGAAATC TGTAAAGCAG GCCCTCCAGG GCCCCCAGGA
    TCTCCAGGTG ACCAAGGACT CCAAGGAGAG CAAGGAGTAA AAGGTGACAA AGGTGATACT
    TGCTTCAACT GTATTGGAAC TGGCATTTCA GGGCCTCCAG GCCAACCAGG TTTACCAGGT
    CTCCCAGGTC CTCCAGGATC TCTTGGAATC CCTGGAGAGA AGGGCGAGAA AGGACAAGCT
    GGGACAACTG GTCCAAAAGG ATTGCCAGGC ATTCCTGGAC CTCCAGGTGC TCCAGGCTTT
    CCAGGGTCTA AGGGTGACCC TGGTGATGTT CTCACTCTTC CGGGAATGAA AGGTGAAAAA
    GGAGAATTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTC TACCTGGTAG TCCTGGAAAG
    GATGGTTTGC CAGGACTTCC TGGCCCCAAA GGAGAGCCCG GTGGAATCAC TTTTAAGGGT
    GAAAGGGGTC CTCCTGGGAA CCCAGGTTTA CCAGGTCTCC CAGGAAATAT GGGACCTACA
    GGCCCTGTTG GTTTTGGGCC TCCAGGCCCC GTAGGTGAAA AAGGCATACA AGGTGTGGCG
    GGAAATCCAG GCCAGCCAGG ATTACCAGGT CCTAAAGGTG ATCCAGGCCA GACCATCAGC
    CAGCCAGGAA AGCCTGGCTT GCCAGGGAAT CCAGGCAGAG ATGGTGAAGT GGGTCTTCCA
    GGTGATCCTG GACTTCCAGG CCAACCAGGC CTACCAGGAA TACCTGGTAG CAAAGGAGAA
    CCAGGTATCC CTGGGATTGG GCCTCCAGGA CCACCTGGTC CCAAAGGTTT TCCAGGAATT
    CCTGGACCTC CAGGACCTCC TGGGGCCCCT GGAAGAATGG GCCCAGAAGG GCCTCCTGGG
    TTACCGGGCT TTCCAGGACC CAAGGGAGAG CCAGGATTTG GGTTACCTGG GCCACCTGGG
    CCACCAGGAT TCCCAGGTTT CAAAGGAACA CCTGGTCCAA AAGGTGATCG TGGTTTACCA
    GGACCTCCAG GTCCTCCAGG ACACACTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAT
    GCTGGACCAA ATGGACAACC TGGCCCAATA GGGCCACCAG GGCTGCCAGG AATTGGTCTT
    CAGGGACCAC CAGGACCACC AGGGACTCCA GGGCCAATAG GCCAACCTGG CTTACATGGA
    ATACCAGGAG AGAAGGGAGA TCCAGGACCT CCTGGGCTTG ATGTTCCAGG ACTCCCTGGA
    GAGAGAGGTA GTCCAGGGCT CCCTGGAGCC CCTGGTCTTA TAGGACCTCC AGGATCACCT
    GGTATCCCTG GAAGAGCAGG TGTCCCTGGA TTTCCAGGTG TCAAAGGCGA AATGGGTATG
    ATGGGACCTC CAGGCCCACC GGGACCTTTG GGTACTCCTG GCAGGAGTGG TGCTCCTGGT
    CTTAAAGGTG ATGACGGCAT GCAAGGTCAG CCAGGTCTCC CTGGCCCTGT AGGAGAAAAG
    GGTGGTAAGG GAGAACCTGG CCTTCCAGGT CCTCCTGGAC CAATGCGTCC AGATTTCCTG
    GGATCAAAAG GAGAAAAGGG TGATCCTGGC TTACCAGGTA TTCCTGGACT TTCCGGACCA
    AAAGGCTACC AGGGATTACC TGGAGACCCA GGGCAACCTG GGCTGAGTGG ACAGCCGGGA
    TCACCAGGAC TACCAGGTCC CAAAGGTACT CCTGGTCTCC CTGGTCCACC AGGACTTACC
    GGACCTCCTG GACCTAAAGG AAACATTGGC GACATGGGTT TTCCTGGCCC TCAAGGTGTA
    GATGGGCCTC CTGGACCTCC TGGAGGTCCT GGACAACCTG GCTCTCCCGG GTTGCCTGGC
    CAGAAAGGAA GTAAAGGAGA ACCTGGAGTT TCAGGCATTG GTCTCCCTGG TCTTCCTGGT
    CCAAAGGGTG AACCTGGTCT GCCTGGATAT CCAGGAAACC CAGGTATCAA AGGTTCTATG
    GGCGAGGCTG GTCTGCCTGG CTTACCAGGA AGCCCTGGAG CAAAAGGTCA ACCCGGCCTG
    CCCGGATTCC CAGGAACACC AGGGCTTCCT GGACCAAAGG GTATGAATGG TCCTCCTGGT
    AACCCTGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGGCGTCC TGGACCACCA
    GGACCTCCTG GTGAAAAAGG CAAGCCAGGT CAAGATGGCA TTCCTGGACC AGCAGGACAA
    AAGGGAGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CTCCTGGACT CCCAGGACTT
    TCTGGTCAAA AGGGTGATGG AGGAGCCCCT GGCCTTCCAG GAAATCCTGG CCTTCCAGGT
    TCAAAGGGTG AACCAGGCTT TCAGGGATTC CCTGGTATGC CAGGTCCCCC AGGTCCCCCT
    GGTTCTCCTG GCCCATCTTT GGAAGGTCCC AAAGGAAACC CTGGGCCTCA AGGCCCTCCT
    GGGAGACCAG GTCCTCCAGG TTTTCAAGGT CCACCTGGTA CAGAAGGTCC TCGTGGCCCC
    CCTGGAAATG GAGGTATTAA AGGAGAGAGG GGAAATCCAG GTCCACCTGG GCAACCAGGC
    TTACCTGGCT TGAAAGGAGA TCAAGGACCA CCAGGCCTTC CGGGTAACCC TGGCCGACCA
    GGTCTTAATG GAATGAAAGG AGATCCTGGT CTCCCTGGTG TTCCAGGATT CCCAGGAATG
    AAAGGACCCA TTGGAGTACC AGGCTCAACT GGCCCTGATG GGGAACCAGG TCTTACGGGG
    CCCCCAGGTC CTCCTGGGTT GCCTGGTCCT TCAGGACAGA GTATTGTAAT CAAAGGAGAT
    CCTGGACCTC CAGGAATTCC TGGACAACCT GGATTAAAAG GTCTACCAGG ACTTCCAGGC
    CCTCAAGGTC TACCAGGTCC AATTGGCCCT CCAGGAGATC CAGGACGCAA TGGACTCCCA
    GGCTTTGATG GTGCAGGAGG GCGCAAAGGA GACCCAGGCC TGCCGGGGCA GCCAGGTAGC
    CGTGGATTGG ATGGTCCCCC AGGACCAGAT GGATTGCAAG GACCTCCAGG GCCCCCGGGA
    ACCACCTCTG TTGCCCATGG ATTCCTCATC ACACGTCACA GTCAGACAAC AGATGCACCA
    CAATGCCCAC AGGGAACAGT CCATATCTAT GATGGCTTTT CTCTCTTATA TGTACAAGGA
    AATAAAAGAG CCCATGGTCA AGACTTGGGG ACAGCTGGCA GCTGCCTTCG TCGCTTCAGT
    ACCATGCCTT TCATGTTCTG CAACATTAAC AACGTTTGTA ACTTTGCTTC AAGAAATGAC
    TATTCTTACT GGCTTTCCAC CCCAGAGCCC ATGCCAATGA ACATGGAACC CCTGAAGGGC
    CAGAGCATCC AGCCATTCAT TAGTCGATGT GCAGTATGTG AGGCACCAGC TGTGGTGATT
    GCAGTTCACA GCCAGACTAT TCAGATTCCT CACTGTCCTC AGGGGTGGGA CTCTCTGTGG
    ATTGGCTATT CATTCATGAT GCATACAAGT GCAGGAGCAG AAGGCTCTGG CCAAGCCCTA
    GCCTCTCCTG GCTCCTGTTT GGAAGAATTT CGTTCAGCTC CCTTCATCGA ATGTCATGGA
    CGAGGGACAT GCAATTACTA TGCCAATTCT TACAGCTTTT GGCTGGCCAC TGTTGATATG
    TCAGACATGT TCAGTAAACC TCAGTCAGAG ACACTGAAAG CAGGCGACTT GAGGACACGT
    ATTAGCCGAT GTCAAGTATG CATGAAAAGG ACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa21617
    Clone ID Related Accession (Same CDS sequence) XM_343778.6 , XM_001055156.4
    Accession Version XM_001055156.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5076bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product collagen alpha-5(IV) chain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000089.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 9, 2013 this sequence version replaced XM_001055156.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCAAGTGC CTGGAGTGTG CCTGGCCGCC GGCTGGTTCT TACTGGCCCT GTGTCTGTGG 
    GGGCAGCCGG GAGAGGCTGC GGCCTGCCAT GGGTGTTCTT CTGGATCAAA ATGTGACTGC
    AGTGGTATAA AAGGAGAAAA GGGAGAACGG GGCTTTCCAG GCTTAGAAGG CCATCCAGGT
    TTACCAGGAT TTCCAGGTCC AGAAGGGCCT CCAGGACCTC GGGGACAAAA GGGTGATGAT
    GGAATTCCAG GGCCACCAGG ACCAAAAGGA ATCAGAGGTC CTCCTGGACT TCCTGGATTT
    CCAGGGACAC CAGGCCTTCC TGGGATGCCA GGCCACGATG GGGCTCCAGG ACCTCAAGGT
    ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGCGGATTTC CAGGCAGTCC AGGCTTTCCC
    GGTTTACAAG GACCTCCAGG ACCACCTGGG ATCCCAGGTA TGAAGGGGGA GCCAGGCAGT
    ATAATCATGT CATCTCTGCC AGGGCCAAAG GGTAATCAAG GATACCCAGG TCCTCCTGGA
    ATACAAGGTC CACCTGGTCC CAAGGGTGTA TCAGGGCCAA GTGGTCCTCC AGGACCACCA
    GGTTTAATGG GCCCTCCTGG CCCACCAGGC CCACCGGGAC CAAAGGGGAA TATGGGCTTA
    AATTTCCAGG GACCCAAAGG CGAAAAAGGT GAACAAGGTC TTCAGGGTCC ACCTGGTCCA
    CCTGGGCAGA TCAGTGAACA GAAAAGACCA ATTGACCTAG ATTTTCAGAA AGGCGATCAG
    GGAGTTCCCG GTGATAGAGG GCCTCCTGGA CCTCCGGGAA TACCTGGTCC TCCCGGTCCT
    CCTGGTGGTG TGAAAGGTGA AAAGGGTGAG CCCGGAGAGC CAGGCAAAAG AGGTAAACCA
    GGCAAAGATG GAGAGACTGG TCAACCAGGA ATCCCAGGTT TGCCTGGTGA TCCTGGTTAC
    CCTGGTGAAC CAGGAAGGCA TGGTGAAAAG GGCCAAAAGG GTGATACTGG CCCACCTGGA
    CCACCTGGAT TTGTAATTCC TACACCTGGA ACTGGTGTGA CTATTGGAGC AAAAGGAAAT
    ATTGGGCAAC CTGGTTTGCC TGGAGAAAAA GGAGAGCGAG GATTTCCTGG CATACAAGGT
    CCACCTGGCT TTCCTGGACC TCCAGGGACA GCTGTCATGG GTCCCCCTGG TCCTCCTGGA
    TATCCTGGTG AAAGGGGCCA GAAAGGTGAT GAAGGTCCCC CTGGAATTTG TATTCCTGGA
    TCTCCTGGAC TTGATGGACA GCCTGGAGCT CCTGGCTTTC CAGGACCTCC TGGTCCTCCT
    GGCCCCCAGT TACCCCCGAG TGATGAAATC TGTAAAGCAG GCCCTCCAGG GCCCCCAGGA
    TCTCCAGGTG ACCAAGGACT CCAAGGAGAG CAAGGAGTAA AAGGTGACAA AGGTGATACT
    TGCTTCAACT GTATTGGAAC TGGCATTTCA GGGCCTCCAG GCCAACCAGG TTTACCAGGT
    CTCCCAGGTC CTCCAGGATC TCTTGGAATC CCTGGAGAGA AGGGCGAGAA AGGACAAGCT
    GGGACAACTG GTCCAAAAGG ATTGCCAGGC ATTCCTGGAC CTCCAGGTGC TCCAGGCTTT
    CCAGGGTCTA AGGGTGACCC TGGTGATGTT CTCACTCTTC CGGGAATGAA AGGTGAAAAA
    GGAGAATTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTC TACCTGGTAG TCCTGGAAAG
    GATGGTTTGC CAGGACTTCC TGGCCCCAAA GGAGAGCCCG GTGGAATCAC TTTTAAGGGT
    GAAAGGGGTC CTCCTGGGAA CCCAGGTTTA CCAGGTCTCC CAGGAAATAT GGGACCTACA
    GGCCCTGTTG GTTTTGGGCC TCCAGGCCCC GTAGGTGAAA AAGGCATACA AGGTGTGGCG
    GGAAATCCAG GCCAGCCAGG ATTACCAGGT CCTAAAGGTG ATCCAGGCCA GACCATCAGC
    CAGCCAGGAA AGCCTGGCTT GCCAGGGAAT CCAGGCAGAG ATGGTGAAGT GGGTCTTCCA
    GGTGATCCTG GACTTCCAGG CCAACCAGGC CTACCAGGAA TACCTGGTAG CAAAGGAGAA
    CCAGGTATCC CTGGGATTGG GCCTCCAGGA CCACCTGGTC CCAAAGGTTT TCCAGGAATT
    CCTGGACCTC CAGGACCTCC TGGGGCCCCT GGAAGAATGG GCCCAGAAGG GCCTCCTGGG
    TTACCGGGCT TTCCAGGACC CAAGGGAGAG CCAGGATTTG GGTTACCTGG GCCACCTGGG
    CCACCAGGAT TCCCAGGTTT CAAAGGAACA CCTGGTCCAA AAGGTGATCG TGGTTTACCA
    GGACCTCCAG GTCCTCCAGG ACACACTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAT
    GCTGGACCAA ATGGACAACC TGGCCCAATA GGGCCACCAG GGCTGCCAGG AATTGGTCTT
    CAGGGACCAC CAGGACCACC AGGGACTCCA GGGCCAATAG GCCAACCTGG CTTACATGGA
    ATACCAGGAG AGAAGGGAGA TCCAGGACCT CCTGGGCTTG ATGTTCCAGG ACTCCCTGGA
    GAGAGAGGTA GTCCAGGGCT CCCTGGAGCC CCTGGTCTTA TAGGACCTCC AGGATCACCT
    GGTATCCCTG GAAGAGCAGG TGTCCCTGGA TTTCCAGGTG TCAAAGGCGA AATGGGTATG
    ATGGGACCTC CAGGCCCACC GGGACCTTTG GGTACTCCTG GCAGGAGTGG TGCTCCTGGT
    CTTAAAGGTG ATGACGGCAT GCAAGGTCAG CCAGGTCTCC CTGGCCCTGT AGGAGAAAAG
    GGTGGTAAGG GAGAACCTGG CCTTCCAGGT CCTCCTGGAC CAATGCGTCC AGATTTCCTG
    GGATCAAAAG GAGAAAAGGG TGATCCTGGC TTACCAGGTA TTCCTGGACT TTCCGGACCA
    AAAGGCTACC AGGGATTACC TGGAGACCCA GGGCAACCTG GGCTGAGTGG ACAGCCGGGA
    TCACCAGGAC TACCAGGTCC CAAAGGTACT CCTGGTCTCC CTGGTCCACC AGGACTTACC
    GGACCTCCTG GACCTAAAGG AAACATTGGC GACATGGGTT TTCCTGGCCC TCAAGGTGTA
    GATGGGCCTC CTGGACCTCC TGGAGGTCCT GGACAACCTG GCTCTCCCGG GTTGCCTGGC
    CAGAAAGGAA GTAAAGGAGA ACCTGGAGTT TCAGGCATTG GTCTCCCTGG TCTTCCTGGT
    CCAAAGGGTG AACCTGGTCT GCCTGGATAT CCAGGAAACC CAGGTATCAA AGGTTCTATG
    GGCGAGGCTG GTCTGCCTGG CTTACCAGGA AGCCCTGGAG CAAAAGGTCA ACCCGGCCTG
    CCCGGATTCC CAGGAACACC AGGGCTTCCT GGACCAAAGG GTATGAATGG TCCTCCTGGT
    AACCCTGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGGCGTCC TGGACCACCA
    GGACCTCCTG GTGAAAAAGG CAAGCCAGGT CAAGATGGCA TTCCTGGACC AGCAGGACAA
    AAGGGAGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CTCCTGGACT CCCAGGACTT
    TCTGGTCAAA AGGGTGATGG AGGAGCCCCT GGCCTTCCAG GAAATCCTGG CCTTCCAGGT
    TCAAAGGGTG AACCAGGCTT TCAGGGATTC CCTGGTATGC CAGGTCCCCC AGGTCCCCCT
    GGTTCTCCTG GCCCATCTTT GGAAGGTCCC AAAGGAAACC CTGGGCCTCA AGGCCCTCCT
    GGGAGACCAG GTCCTCCAGG TTTTCAAGGT CCACCTGGTA CAGAAGGTCC TCGTGGCCCC
    CCTGGAAATG GAGGTATTAA AGGAGAGAGG GGAAATCCAG GTCCACCTGG GCAACCAGGC
    TTACCTGGCT TGAAAGGAGA TCAAGGACCA CCAGGCCTTC CGGGTAACCC TGGCCGACCA
    GGTCTTAATG GAATGAAAGG AGATCCTGGT CTCCCTGGTG TTCCAGGATT CCCAGGAATG
    AAAGGACCCA TTGGAGTACC AGGCTCAACT GGCCCTGATG GGGAACCAGG TCTTACGGGG
    CCCCCAGGTC CTCCTGGGTT GCCTGGTCCT TCAGGACAGA GTATTGTAAT CAAAGGAGAT
    CCTGGACCTC CAGGAATTCC TGGACAACCT GGATTAAAAG GTCTACCAGG ACTTCCAGGC
    CCTCAAGGTC TACCAGGTCC AATTGGCCCT CCAGGAGATC CAGGACGCAA TGGACTCCCA
    GGCTTTGATG GTGCAGGAGG GCGCAAAGGA GACCCAGGCC TGCCGGGGCA GCCAGGTAGC
    CGTGGATTGG ATGGTCCCCC AGGACCAGAT GGATTGCAAG GACCTCCAGG GCCCCCGGGA
    ACCACCTCTG TTGCCCATGG ATTCCTCATC ACACGTCACA GTCAGACAAC AGATGCACCA
    CAATGCCCAC AGGGAACAGT CCATATCTAT GATGGCTTTT CTCTCTTATA TGTACAAGGA
    AATAAAAGAG CCCATGGTCA AGACTTGGGG ACAGCTGGCA GCTGCCTTCG TCGCTTCAGT
    ACCATGCCTT TCATGTTCTG CAACATTAAC AACGTTTGTA ACTTTGCTTC AAGAAATGAC
    TATTCTTACT GGCTTTCCAC CCCAGAGCCC ATGCCAATGA ACATGGAACC CCTGAAGGGC
    CAGAGCATCC AGCCATTCAT TAGTCGATGT GCAGTATGTG AGGCACCAGC TGTGGTGATT
    GCAGTTCACA GCCAGACTAT TCAGATTCCT CACTGTCCTC AGGGGTGGGA CTCTCTGTGG
    ATTGGCTATT CATTCATGAT GCATACAAGT GCAGGAGCAG AAGGCTCTGG CCAAGCCCTA
    GCCTCTCCTG GCTCCTGTTT GGAAGAATTT CGTTCAGCTC CCTTCATCGA ATGTCATGGA
    CGAGGGACAT GCAATTACTA TGCCAATTCT TACAGCTTTT GGCTGGCCAC TGTTGATATG
    TCAGACATGT TCAGTAAACC TCAGTCAGAG ACACTGAAAG CAGGCGACTT GAGGACACGT
    ATTAGCCGAT GTCAAGTATG CATGAAAAGG ACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001055156.1
    CDS400..5475
    Translation

    Target ORF information:

    RefSeq Version XM_001055156.4
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus collagen type IV alpha 5 chain (Col4a5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_001055156.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCAAGTGC CTGGAGTGTG CCTGGCCGCC GGCTGGTTCT TACTGGCCCT GTGTCTGTGG 
    GGGCAGCCGG GAGAGGCTGC GGCCTGCCAT GGGTGTTCTT CTGGATCAAA ATGTGACTGC
    AGTGGTATAA AAGGAGAAAA GGGAGAACGG GGCTTTCCAG GCTTAGAAGG CCATCCAGGT
    TTACCAGGAT TTCCAGGTCC AGAAGGGCCT CCAGGACCTC GGGGACAAAA GGGTGATGAT
    GGAATTCCAG GGCCACCAGG ACCAAAAGGA ATCAGAGGTC CTCCTGGACT TCCTGGATTT
    CCAGGGACAC CAGGCCTTCC TGGGATGCCA GGCCACGATG GGGCTCCAGG ACCTCAAGGT
    ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGCGGATTTC CAGGCAGTCC AGGCTTTCCC
    GGTTTACAAG GACCTCCAGG ACCACCTGGG ATCCCAGGTA TGAAGGGGGA GCCAGGCAGT
    ATAATCATGT CATCTCTGCC AGGGCCAAAG GGTAATCAAG GATACCCAGG TCCTCCTGGA
    ATACAAGGTC CACCTGGTCC CAAGGGTGTA TCAGGGCCAA GTGGTCCTCC AGGACCACCA
    GGTTTAATGG GCCCTCCTGG CCCACCAGGC CCACCGGGAC CAAAGGGGAA TATGGGCTTA
    AATTTCCAGG GACCCAAAGG CGAAAAAGGT GAACAAGGTC TTCAGGGTCC ACCTGGTCCA
    CCTGGGCAGA TCAGTGAACA GAAAAGACCA ATTGACCTAG ATTTTCAGAA AGGCGATCAG
    GGAGTTCCCG GTGATAGAGG GCCTCCTGGA CCTCCGGGAA TACCTGGTCC TCCCGGTCCT
    CCTGGTGGTG TGAAAGGTGA AAAGGGTGAG CCCGGAGAGC CAGGCAAAAG AGGTAAACCA
    GGCAAAGATG GAGAGACTGG TCAACCAGGA ATCCCAGGTT TGCCTGGTGA TCCTGGTTAC
    CCTGGTGAAC CAGGAAGGCA TGGTGAAAAG GGCCAAAAGG GTGATACTGG CCCACCTGGA
    CCACCTGGAT TTGTAATTCC TACACCTGGA ACTGGTGTGA CTATTGGAGC AAAAGGAAAT
    ATTGGGCAAC CTGGTTTGCC TGGAGAAAAA GGAGAGCGAG GATTTCCTGG CATACAAGGT
    CCACCTGGCT TTCCTGGACC TCCAGGGACA GCTGTCATGG GTCCCCCTGG TCCTCCTGGA
    TATCCTGGTG AAAGGGGCCA GAAAGGTGAT GAAGGTCCCC CTGGAATTTG TATTCCTGGA
    TCTCCTGGAC TTGATGGACA GCCTGGAGCT CCTGGCTTTC CAGGACCTCC TGGTCCTCCT
    GGCCCCCAGT TACCCCCGAG TGATGAAATC TGTAAAGCAG GCCCTCCAGG GCCCCCAGGA
    TCTCCAGGTG ACCAAGGACT CCAAGGAGAG CAAGGAGTAA AAGGTGACAA AGGTGATACT
    TGCTTCAACT GTATTGGAAC TGGCATTTCA GGGCCTCCAG GCCAACCAGG TTTACCAGGT
    CTCCCAGGTC CTCCAGGATC TCTTGGAATC CCTGGAGAGA AGGGCGAGAA AGGACAAGCT
    GGGACAACTG GTCCAAAAGG ATTGCCAGGC ATTCCTGGAC CTCCAGGTGC TCCAGGCTTT
    CCAGGGTCTA AGGGTGACCC TGGTGATGTT CTCACTCTTC CGGGAATGAA AGGTGAAAAA
    GGAGAATTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTC TACCTGGTAG TCCTGGAAAG
    GATGGTTTGC CAGGACTTCC TGGCCCCAAA GGAGAGCCCG GTGGAATCAC TTTTAAGGGT
    GAAAGGGGTC CTCCTGGGAA CCCAGGTTTA CCAGGTCTCC CAGGAAATAT GGGACCTACA
    GGCCCTGTTG GTTTTGGGCC TCCAGGCCCC GTAGGTGAAA AAGGCATACA AGGTGTGGCG
    GGAAATCCAG GCCAGCCAGG ATTACCAGGT CCTAAAGGTG ATCCAGGCCA GACCATCAGC
    CAGCCAGGAA AGCCTGGCTT GCCAGGGAAT CCAGGCAGAG ATGGTGAAGT GGGTCTTCCA
    GGTGATCCTG GACTTCCAGG CCAACCAGGC CTACCAGGAA TACCTGGTAG CAAAGGAGAA
    CCAGGTATCC CTGGGATTGG GCCTCCAGGA CCACCTGGTC CCAAAGGTTT TCCAGGAATT
    CCTGGACCTC CAGGACCTCC TGGGGCCCCT GGAAGAATGG GCCCAGAAGG GCCTCCTGGG
    TTACCGGGCT TTCCAGGACC CAAGGGAGAG CCAGGATTTG GGTTACCTGG GCCACCTGGG
    CCACCAGGAT TCCCAGGTTT CAAAGGAACA CCTGGTCCAA AAGGTGATCG TGGTTTACCA
    GGACCTCCAG GTCCTCCAGG ACACACTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAT
    GCTGGACCAA ATGGACAACC TGGCCCAATA GGGCCACCAG GGCTGCCAGG AATTGGTCTT
    CAGGGACCAC CAGGACCACC AGGGACTCCA GGGCCAATAG GCCAACCTGG CTTACATGGA
    ATACCAGGAG AGAAGGGAGA TCCAGGACCT CCTGGGCTTG ATGTTCCAGG ACTCCCTGGA
    GAGAGAGGTA GTCCAGGGCT CCCTGGAGCC CCTGGTCTTA TAGGACCTCC AGGATCACCT
    GGTATCCCTG GAAGAGCAGG TGTCCCTGGA TTTCCAGGTG TCAAAGGCGA AATGGGTATG
    ATGGGACCTC CAGGCCCACC GGGACCTTTG GGTACTCCTG GCAGGAGTGG TGCTCCTGGT
    CTTAAAGGTG ATGACGGCAT GCAAGGTCAG CCAGGTCTCC CTGGCCCTGT AGGAGAAAAG
    GGTGGTAAGG GAGAACCTGG CCTTCCAGGT CCTCCTGGAC CAATGCGTCC AGATTTCCTG
    GGATCAAAAG GAGAAAAGGG TGATCCTGGC TTACCAGGTA TTCCTGGACT TTCCGGACCA
    AAAGGCTACC AGGGATTACC TGGAGACCCA GGGCAACCTG GGCTGAGTGG ACAGCCGGGA
    TCACCAGGAC TACCAGGTCC CAAAGGTACT CCTGGTCTCC CTGGTCCACC AGGACTTACC
    GGACCTCCTG GACCTAAAGG AAACATTGGC GACATGGGTT TTCCTGGCCC TCAAGGTGTA
    GATGGGCCTC CTGGACCTCC TGGAGGTCCT GGACAACCTG GCTCTCCCGG GTTGCCTGGC
    CAGAAAGGAA GTAAAGGAGA ACCTGGAGTT TCAGGCATTG GTCTCCCTGG TCTTCCTGGT
    CCAAAGGGTG AACCTGGTCT GCCTGGATAT CCAGGAAACC CAGGTATCAA AGGTTCTATG
    GGCGAGGCTG GTCTGCCTGG CTTACCAGGA AGCCCTGGAG CAAAAGGTCA ACCCGGCCTG
    CCCGGATTCC CAGGAACACC AGGGCTTCCT GGACCAAAGG GTATGAATGG TCCTCCTGGT
    AACCCTGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGGCGTCC TGGACCACCA
    GGACCTCCTG GTGAAAAAGG CAAGCCAGGT CAAGATGGCA TTCCTGGACC AGCAGGACAA
    AAGGGAGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CTCCTGGACT CCCAGGACTT
    TCTGGTCAAA AGGGTGATGG AGGAGCCCCT GGCCTTCCAG GAAATCCTGG CCTTCCAGGT
    TCAAAGGGTG AACCAGGCTT TCAGGGATTC CCTGGTATGC CAGGTCCCCC AGGTCCCCCT
    GGTTCTCCTG GCCCATCTTT GGAAGGTCCC AAAGGAAACC CTGGGCCTCA AGGCCCTCCT
    GGGAGACCAG GTCCTCCAGG TTTTCAAGGT CCACCTGGTA CAGAAGGTCC TCGTGGCCCC
    CCTGGAAATG GAGGTATTAA AGGAGAGAGG GGAAATCCAG GTCCACCTGG GCAACCAGGC
    TTACCTGGCT TGAAAGGAGA TCAAGGACCA CCAGGCCTTC CGGGTAACCC TGGCCGACCA
    GGTCTTAATG GAATGAAAGG AGATCCTGGT CTCCCTGGTG TTCCAGGATT CCCAGGAATG
    AAAGGACCCA TTGGAGTACC AGGCTCAACT GGCCCTGATG GGGAACCAGG TCTTACGGGG
    CCCCCAGGTC CTCCTGGGTT GCCTGGTCCT TCAGGACAGA GTATTGTAAT CAAAGGAGAT
    CCTGGACCTC CAGGAATTCC TGGACAACCT GGATTAAAAG GTCTACCAGG ACTTCCAGGC
    CCTCAAGGTC TACCAGGTCC AATTGGCCCT CCAGGAGATC CAGGACGCAA TGGACTCCCA
    GGCTTTGATG GTGCAGGAGG GCGCAAAGGA GACCCAGGCC TGCCGGGGCA GCCAGGTAGC
    CGTGGATTGG ATGGTCCCCC AGGACCAGAT GGATTGCAAG GACCTCCAGG GCCCCCGGGA
    ACCACCTCTG TTGCCCATGG ATTCCTCATC ACACGTCACA GTCAGACAAC AGATGCACCA
    CAATGCCCAC AGGGAACAGT CCATATCTAT GATGGCTTTT CTCTCTTATA TGTACAAGGA
    AATAAAAGAG CCCATGGTCA AGACTTGGGG ACAGCTGGCA GCTGCCTTCG TCGCTTCAGT
    ACCATGCCTT TCATGTTCTG CAACATTAAC AACGTTTGTA ACTTTGCTTC AAGAAATGAC
    TATTCTTACT GGCTTTCCAC CCCAGAGCCC ATGCCAATGA ACATGGAACC CCTGAAGGGC
    CAGAGCATCC AGCCATTCAT TAGTCGATGT GCAGTATGTG AGGCACCAGC TGTGGTGATT
    GCAGTTCACA GCCAGACTAT TCAGATTCCT CACTGTCCTC AGGGGTGGGA CTCTCTGTGG
    ATTGGCTATT CATTCATGAT GCATACAAGT GCAGGAGCAG AAGGCTCTGG CCAAGCCCTA
    GCCTCTCCTG GCTCCTGTTT GGAAGAATTT CGTTCAGCTC CCTTCATCGA ATGTCATGGA
    CGAGGGACAT GCAATTACTA TGCCAATTCT TACAGCTTTT GGCTGGCCAC TGTTGATATG
    TCAGACATGT TCAGTAAACC TCAGTCAGAG ACACTGAAAG CAGGCGACTT GAGGACACGT
    ATTAGCCGAT GTCAAGTATG CATGAAAAGG ACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa21618
    Clone ID Related Accession (Same CDS sequence) XM_006227490.1 , XM_006257312.1
    Accession Version XM_006227490.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5067bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product collagen alpha-5(IV) chain isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000089.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCAAGTGC CTGGAGTGTG CCTGGCCGCC GGCTGGTTCT TACTGGCCCT GTGTCTGTGG 
    GGGCAGCCGG GAGAGGCTGC GGCCTGCCAT GGGTGTTCTT CTGGATCAAA ATGTGACTGC
    AGTGGTATAA AAGGAGAAAA GGGAGAACGG GGCTTTCCAG GCTTAGAAGG CCATCCAGGT
    TTACCAGGAT TTCCAGGTCC AGAAGGGCCT CCAGGACCTC GGGGACAAAA GGGTGATGAT
    GGAATTCCAG GGCCACCAGG ACCAAAAGGA ATCAGAGGTC CTCCTGGACT TCCTGGATTT
    CCAGGGACAC CAGGCCTTCC TGGGATGCCA GGCCACGATG GGGCTCCAGG ACCTCAAGGT
    ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGCGGATTTC CAGGCAGTCC AGGCTTTCCC
    GGTTTACAAG GACCTCCAGG ACCACCTGGG ATCCCAGGTA TGAAGGGGGA GCCAGGCAGT
    ATAATCATGT CATCTCTGCC AGGGCCAAAG GGTAATCAAG GATACCCAGG TCCTCCTGGA
    ATACAAGGTC CACCTGGTCC CAAGGGTGTA TCAGGGCCAA GTGGTCCTCC AGGACCACCA
    GGTTTAATGG GCCCTCCTGG CCCACCAGGC CCACCGGGAC CAAAGGGGAA TATGGGCTTA
    AATTTCCAGG GACCCAAAGG CGAAAAAGGT GAACAAGGTC TTCAGGGTCC ACCTGGTCCA
    CCTGGGCAGA TCAGTGAACA GAAAAGACCA ATTGACCTAG ATTTTCAGAA AGGCGATCAG
    GGAGTTCCCG GTGATAGAGG GCCTCCTGGA CCTCCGGGAA TACCTGGTCC TCCCGGTCCT
    CCTGGTGGTG TGAAAGGTGA AAAGGGTGAG CCCGGAGAGC CAGGCAAAAG AGGTAAACCA
    GGCAAAGATG GAGAGACTGG TCAACCAGGA ATCCCAGGTT TGCCTGGTGA TCCTGGTTAC
    CCTGGTGAAC CAGGAAGGCA TGGTGAAAAG GGCCAAAAGG GTGATACTGG CCCACCTGGA
    CCACCTGGAT TTGTAATTCC TACACCTGGA ACTGGTGTGA CTATTGGAGC AAAAGGAAAT
    ATTGGGCAAC CTGGTTTGCC TGGAGAAAAA GGAGAGCGAG GATTTCCTGG CATACAAGGT
    CCACCTGGCT TTCCTGGACC TCCAGGGACA GCTGTCATGG GTCCCCCTGG TCCTCCTGGA
    TATCCTGGTG AAAGGGGCCA GAAAGGTGAT GAAGGTCCCC CTGGAATTTG TATTCCTGGA
    TCTCCTGGAC TTGATGGACA GCCTGGAGCT CCTGGCTTTC CAGGACCTCC TGGTCCTCCT
    GGCCCCCAGT TACCCCCGAG TGATGAAATC TGTAAAGCAG GCCCTCCAGG GCCCCCAGGA
    TCTCCAGGTG ACCAAGGACT CCAAGGAGAG CAAGGAGTAA AAGGTGACAA AGGTGATACT
    TGCTTCAACT GTATTGGAAC TGGCATTTCA GGGCCTCCAG GCCAACCAGG TTTACCAGGT
    CTCCCAGGTC CTCCAGGATC TCTTGGAATC CCTGGAGAGA AGGGCGAGAA AGGACAAGCT
    GGGACAACTG GTCCAAAAGG ATTGCCAGGC ATTCCTGGAC CTCCAGGTGC TCCAGGCTTT
    CCAGGGTCTA AGGGTGACCC TGGTGATGTT CTCACTCTTC CGGGAATGAA AGGTGAAAAA
    GGAGAATTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTC TACCTGGTAG TCCTGGAAAG
    GATGGTTTGC CAGGACTTCC TGGCCCCAAA GGAGAGCCCG GTGGAATCAC TTTTAAGGGT
    GAAAGGGGTC CTCCTGGGAA CCCAGGTTTA CCAGGTCTCC CAGGAAATAT GGGACCTACA
    GGCCCTGTTG GTTTTGGGCC TCCAGGCCCC GTAGGTGAAA AAGGCATACA AGGTGTGGCG
    GGAAATCCAG GCCAGCCAGG ATTACCAGGT CCTAAAGGTG ATCCAGGCCA GACCATCAGC
    CAGCCAGGAA AGCCTGGCTT GCCAGGGAAT CCAGGCAGAG ATGGTGAAGT GGGTCTTCCA
    GGTGATCCTG GACTTCCAGG CCAACCAGGC CTACCAGGAA TACCTGGTAG CAAAGGAGAA
    CCAGGTATCC CTGGGATTGG GCCTCCAGGA CCACCTGGTC CCAAAGGTTT TCCAGGAATT
    CCTGGACCTC CAGGACCTCC TGGGGCCCCT GGAAGAATGG GCCCAGAAGG GCCTCCTGGG
    TTACCGGGCT TTCCAGGACC CAAGGGAGAG CCAGGATTTG GGTTACCTGG GCCACCTGGG
    CCACCAGGAT TCCCAGGTTT CAAAGGAACA CCTGGTCCAA AAGGTGATCG TGGTTTACCA
    GGACCTCCAG GTCCTCCAGG ACACACTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAT
    GCTGGACCAA ATGGACAACC TGGCCCAATA GGGCCACCAG GGCTGCCAGG AATTGGTCTT
    CAGGGACCAC CAGGACCACC AGGGACTCCA GGGCCAATAG GCCAACCTGG CTTACATGGA
    ATACCAGGAG AGAAGGGAGA TCCAGGACCT CCTGGGCTTG ATGTTCCAGG ACTCCCTGGA
    GAGAGAGGTA GTCCAGGGCT CCCTGGAGCC CCTGGTCTTA TAGGACCTCC AGGATCACCT
    GGTATCCCTG GAAGAGCAGG TGTCCCTGGA TTTCCAGGTG TCAAAGGCGA AATGGGTATG
    ATGGGACCTC CAGGCCCACC GGGACCTTTG GGTACTCCTG GCAGGAGTGG TGCTCCTGGT
    CTTAAAGGTG ATGACGGCAT GCAAGGTCAG CCAGGTCTCC CTGGCCCTGT AGGAGAAAAG
    GGTGGTAAGG GAGAACCTGG CCTTCCAGGT CCTCCTGGAC CAATGCGTCC AGATTTCCTG
    GGATCAAAAG GAGAAAAGGG TGATCCTGGC TTACCAGGTA TTCCTGGACT TTCCGGACCA
    AAAGGCTACC AGGGATTACC TGGAGACCCA GGGCAACCTG GGCTGAGTGG ACAGCCGGGA
    TCACCAGGAC TACCAGGTCC CAAAGGTACT CCTGGTCTCC CTGGTCCACC AGGACTTACC
    GGACCTCCTG GACCTAAAGG AAACATTGGC GACATGGGTT TTCCTGGCCC TCAAGGTGTA
    GATGGGCCTC CTGGACCTCC TGGAGGTCCT GGACAACCTG GCTCTCCCGG GTTGCCTGGC
    CAGAAAGGAA GTAAAGGAGA ACCTGGAGTT TCAGGCATTG GTCTCCCTGG TCTTCCTGGT
    CCAAAGGGTG AACCTGGTCT GCCTGGATAT CCAGGAAACC CAGGTATCAA AGGTTCTATG
    GGCGAGGCTG GTCTGCCTGG CTTACCAGGA AGCCCTGGAG CAAAAGGTCA ACCCGGCCTG
    CCCGGATTCC CAGGAACACC AGGGCTTCCT GGACCAAAGG GTATGAATGG TCCTCCTGGT
    AACCCTGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGGCGTCC TGGACCACCA
    GGACCTCCTG GTGAAAAAGG CAAGCCAGGT CAAGATGGCA TTCCTGGACC AGCAGGACAA
    AAGGGAGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CTCCTGGACT CCCAGGACTT
    TCTGGTCAAA AGGGTGATGG AGGAGCCCCT GGCCTTCCAG GAAATCCTGG CCTTCCAGGT
    TCAAAGGGTG AACCAGGCTT TCAGGGATTC CCTGGTATGC CAGGTCCCCC AGGTCCCCCT
    GGTTCTCCTG GCCCATCTTT GGAAGGTCCC AAAGGAAACC CTGGGCCTCA AGGCCCTCCT
    GGGAGACCAG GTCCTCCAGG TCCACCTGGT ACAGAAGGTC CTCGTGGCCC CCCTGGAAAT
    GGAGGTATTA AAGGAGAGAG GGGAAATCCA GGTCCACCTG GGCAACCAGG CTTACCTGGC
    TTGAAAGGAG ATCAAGGACC ACCAGGCCTT CCGGGTAACC CTGGCCGACC AGGTCTTAAT
    GGAATGAAAG GAGATCCTGG TCTCCCTGGT GTTCCAGGAT TCCCAGGAAT GAAAGGACCC
    ATTGGAGTAC CAGGCTCAAC TGGCCCTGAT GGGGAACCAG GTCTTACGGG GCCCCCAGGT
    CCTCCTGGGT TGCCTGGTCC TTCAGGACAG AGTATTGTAA TCAAAGGAGA TCCTGGACCT
    CCAGGAATTC CTGGACAACC TGGATTAAAA GGTCTACCAG GACTTCCAGG CCCTCAAGGT
    CTACCAGGTC CAATTGGCCC TCCAGGAGAT CCAGGACGCA ATGGACTCCC AGGCTTTGAT
    GGTGCAGGAG GGCGCAAAGG AGACCCAGGC CTGCCGGGGC AGCCAGGTAG CCGTGGATTG
    GATGGTCCCC CAGGACCAGA TGGATTGCAA GGACCTCCAG GGCCCCCGGG AACCACCTCT
    GTTGCCCATG GATTCCTCAT CACACGTCAC AGTCAGACAA CAGATGCACC ACAATGCCCA
    CAGGGAACAG TCCATATCTA TGATGGCTTT TCTCTCTTAT ATGTACAAGG AAATAAAAGA
    GCCCATGGTC AAGACTTGGG GACAGCTGGC AGCTGCCTTC GTCGCTTCAG TACCATGCCT
    TTCATGTTCT GCAACATTAA CAACGTTTGT AACTTTGCTT CAAGAAATGA CTATTCTTAC
    TGGCTTTCCA CCCCAGAGCC CATGCCAATG AACATGGAAC CCCTGAAGGG CCAGAGCATC
    CAGCCATTCA TTAGTCGATG TGCAGTATGT GAGGCACCAG CTGTGGTGAT TGCAGTTCAC
    AGCCAGACTA TTCAGATTCC TCACTGTCCT CAGGGGTGGG ACTCTCTGTG GATTGGCTAT
    TCATTCATGA TGCATACAAG TGCAGGAGCA GAAGGCTCTG GCCAAGCCCT AGCCTCTCCT
    GGCTCCTGTT TGGAAGAATT TCGTTCAGCT CCCTTCATCG AATGTCATGG ACGAGGGACA
    TGCAATTACT ATGCCAATTC TTACAGCTTT TGGCTGGCCA CTGTTGATAT GTCAGACATG
    TTCAGTAAAC CTCAGTCAGA GACACTGAAA GCAGGCGACT TGAGGACACG TATTAGCCGA
    TGTCAAGTAT GCATGAAAAG GACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006227552.1
    CDS400..5466
    Translation

    Target ORF information:

    RefSeq Version XM_006227490.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus collagen type IV alpha 5 chain (Col4a5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006227490.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCAAGTGC CTGGAGTGTG CCTGGCCGCC GGCTGGTTCT TACTGGCCCT GTGTCTGTGG 
    GGGCAGCCGG GAGAGGCTGC GGCCTGCCAT GGGTGTTCTT CTGGATCAAA ATGTGACTGC
    AGTGGTATAA AAGGAGAAAA GGGAGAACGG GGCTTTCCAG GCTTAGAAGG CCATCCAGGT
    TTACCAGGAT TTCCAGGTCC AGAAGGGCCT CCAGGACCTC GGGGACAAAA GGGTGATGAT
    GGAATTCCAG GGCCACCAGG ACCAAAAGGA ATCAGAGGTC CTCCTGGACT TCCTGGATTT
    CCAGGGACAC CAGGCCTTCC TGGGATGCCA GGCCACGATG GGGCTCCAGG ACCTCAAGGT
    ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGCGGATTTC CAGGCAGTCC AGGCTTTCCC
    GGTTTACAAG GACCTCCAGG ACCACCTGGG ATCCCAGGTA TGAAGGGGGA GCCAGGCAGT
    ATAATCATGT CATCTCTGCC AGGGCCAAAG GGTAATCAAG GATACCCAGG TCCTCCTGGA
    ATACAAGGTC CACCTGGTCC CAAGGGTGTA TCAGGGCCAA GTGGTCCTCC AGGACCACCA
    GGTTTAATGG GCCCTCCTGG CCCACCAGGC CCACCGGGAC CAAAGGGGAA TATGGGCTTA
    AATTTCCAGG GACCCAAAGG CGAAAAAGGT GAACAAGGTC TTCAGGGTCC ACCTGGTCCA
    CCTGGGCAGA TCAGTGAACA GAAAAGACCA ATTGACCTAG ATTTTCAGAA AGGCGATCAG
    GGAGTTCCCG GTGATAGAGG GCCTCCTGGA CCTCCGGGAA TACCTGGTCC TCCCGGTCCT
    CCTGGTGGTG TGAAAGGTGA AAAGGGTGAG CCCGGAGAGC CAGGCAAAAG AGGTAAACCA
    GGCAAAGATG GAGAGACTGG TCAACCAGGA ATCCCAGGTT TGCCTGGTGA TCCTGGTTAC
    CCTGGTGAAC CAGGAAGGCA TGGTGAAAAG GGCCAAAAGG GTGATACTGG CCCACCTGGA
    CCACCTGGAT TTGTAATTCC TACACCTGGA ACTGGTGTGA CTATTGGAGC AAAAGGAAAT
    ATTGGGCAAC CTGGTTTGCC TGGAGAAAAA GGAGAGCGAG GATTTCCTGG CATACAAGGT
    CCACCTGGCT TTCCTGGACC TCCAGGGACA GCTGTCATGG GTCCCCCTGG TCCTCCTGGA
    TATCCTGGTG AAAGGGGCCA GAAAGGTGAT GAAGGTCCCC CTGGAATTTG TATTCCTGGA
    TCTCCTGGAC TTGATGGACA GCCTGGAGCT CCTGGCTTTC CAGGACCTCC TGGTCCTCCT
    GGCCCCCAGT TACCCCCGAG TGATGAAATC TGTAAAGCAG GCCCTCCAGG GCCCCCAGGA
    TCTCCAGGTG ACCAAGGACT CCAAGGAGAG CAAGGAGTAA AAGGTGACAA AGGTGATACT
    TGCTTCAACT GTATTGGAAC TGGCATTTCA GGGCCTCCAG GCCAACCAGG TTTACCAGGT
    CTCCCAGGTC CTCCAGGATC TCTTGGAATC CCTGGAGAGA AGGGCGAGAA AGGACAAGCT
    GGGACAACTG GTCCAAAAGG ATTGCCAGGC ATTCCTGGAC CTCCAGGTGC TCCAGGCTTT
    CCAGGGTCTA AGGGTGACCC TGGTGATGTT CTCACTCTTC CGGGAATGAA AGGTGAAAAA
    GGAGAATTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTC TACCTGGTAG TCCTGGAAAG
    GATGGTTTGC CAGGACTTCC TGGCCCCAAA GGAGAGCCCG GTGGAATCAC TTTTAAGGGT
    GAAAGGGGTC CTCCTGGGAA CCCAGGTTTA CCAGGTCTCC CAGGAAATAT GGGACCTACA
    GGCCCTGTTG GTTTTGGGCC TCCAGGCCCC GTAGGTGAAA AAGGCATACA AGGTGTGGCG
    GGAAATCCAG GCCAGCCAGG ATTACCAGGT CCTAAAGGTG ATCCAGGCCA GACCATCAGC
    CAGCCAGGAA AGCCTGGCTT GCCAGGGAAT CCAGGCAGAG ATGGTGAAGT GGGTCTTCCA
    GGTGATCCTG GACTTCCAGG CCAACCAGGC CTACCAGGAA TACCTGGTAG CAAAGGAGAA
    CCAGGTATCC CTGGGATTGG GCCTCCAGGA CCACCTGGTC CCAAAGGTTT TCCAGGAATT
    CCTGGACCTC CAGGACCTCC TGGGGCCCCT GGAAGAATGG GCCCAGAAGG GCCTCCTGGG
    TTACCGGGCT TTCCAGGACC CAAGGGAGAG CCAGGATTTG GGTTACCTGG GCCACCTGGG
    CCACCAGGAT TCCCAGGTTT CAAAGGAACA CCTGGTCCAA AAGGTGATCG TGGTTTACCA
    GGACCTCCAG GTCCTCCAGG ACACACTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAT
    GCTGGACCAA ATGGACAACC TGGCCCAATA GGGCCACCAG GGCTGCCAGG AATTGGTCTT
    CAGGGACCAC CAGGACCACC AGGGACTCCA GGGCCAATAG GCCAACCTGG CTTACATGGA
    ATACCAGGAG AGAAGGGAGA TCCAGGACCT CCTGGGCTTG ATGTTCCAGG ACTCCCTGGA
    GAGAGAGGTA GTCCAGGGCT CCCTGGAGCC CCTGGTCTTA TAGGACCTCC AGGATCACCT
    GGTATCCCTG GAAGAGCAGG TGTCCCTGGA TTTCCAGGTG TCAAAGGCGA AATGGGTATG
    ATGGGACCTC CAGGCCCACC GGGACCTTTG GGTACTCCTG GCAGGAGTGG TGCTCCTGGT
    CTTAAAGGTG ATGACGGCAT GCAAGGTCAG CCAGGTCTCC CTGGCCCTGT AGGAGAAAAG
    GGTGGTAAGG GAGAACCTGG CCTTCCAGGT CCTCCTGGAC CAATGCGTCC AGATTTCCTG
    GGATCAAAAG GAGAAAAGGG TGATCCTGGC TTACCAGGTA TTCCTGGACT TTCCGGACCA
    AAAGGCTACC AGGGATTACC TGGAGACCCA GGGCAACCTG GGCTGAGTGG ACAGCCGGGA
    TCACCAGGAC TACCAGGTCC CAAAGGTACT CCTGGTCTCC CTGGTCCACC AGGACTTACC
    GGACCTCCTG GACCTAAAGG AAACATTGGC GACATGGGTT TTCCTGGCCC TCAAGGTGTA
    GATGGGCCTC CTGGACCTCC TGGAGGTCCT GGACAACCTG GCTCTCCCGG GTTGCCTGGC
    CAGAAAGGAA GTAAAGGAGA ACCTGGAGTT TCAGGCATTG GTCTCCCTGG TCTTCCTGGT
    CCAAAGGGTG AACCTGGTCT GCCTGGATAT CCAGGAAACC CAGGTATCAA AGGTTCTATG
    GGCGAGGCTG GTCTGCCTGG CTTACCAGGA AGCCCTGGAG CAAAAGGTCA ACCCGGCCTG
    CCCGGATTCC CAGGAACACC AGGGCTTCCT GGACCAAAGG GTATGAATGG TCCTCCTGGT
    AACCCTGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGGCGTCC TGGACCACCA
    GGACCTCCTG GTGAAAAAGG CAAGCCAGGT CAAGATGGCA TTCCTGGACC AGCAGGACAA
    AAGGGAGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CTCCTGGACT CCCAGGACTT
    TCTGGTCAAA AGGGTGATGG AGGAGCCCCT GGCCTTCCAG GAAATCCTGG CCTTCCAGGT
    TCAAAGGGTG AACCAGGCTT TCAGGGATTC CCTGGTATGC CAGGTCCCCC AGGTCCCCCT
    GGTTCTCCTG GCCCATCTTT GGAAGGTCCC AAAGGAAACC CTGGGCCTCA AGGCCCTCCT
    GGGAGACCAG GTCCTCCAGG TCCACCTGGT ACAGAAGGTC CTCGTGGCCC CCCTGGAAAT
    GGAGGTATTA AAGGAGAGAG GGGAAATCCA GGTCCACCTG GGCAACCAGG CTTACCTGGC
    TTGAAAGGAG ATCAAGGACC ACCAGGCCTT CCGGGTAACC CTGGCCGACC AGGTCTTAAT
    GGAATGAAAG GAGATCCTGG TCTCCCTGGT GTTCCAGGAT TCCCAGGAAT GAAAGGACCC
    ATTGGAGTAC CAGGCTCAAC TGGCCCTGAT GGGGAACCAG GTCTTACGGG GCCCCCAGGT
    CCTCCTGGGT TGCCTGGTCC TTCAGGACAG AGTATTGTAA TCAAAGGAGA TCCTGGACCT
    CCAGGAATTC CTGGACAACC TGGATTAAAA GGTCTACCAG GACTTCCAGG CCCTCAAGGT
    CTACCAGGTC CAATTGGCCC TCCAGGAGAT CCAGGACGCA ATGGACTCCC AGGCTTTGAT
    GGTGCAGGAG GGCGCAAAGG AGACCCAGGC CTGCCGGGGC AGCCAGGTAG CCGTGGATTG
    GATGGTCCCC CAGGACCAGA TGGATTGCAA GGACCTCCAG GGCCCCCGGG AACCACCTCT
    GTTGCCCATG GATTCCTCAT CACACGTCAC AGTCAGACAA CAGATGCACC ACAATGCCCA
    CAGGGAACAG TCCATATCTA TGATGGCTTT TCTCTCTTAT ATGTACAAGG AAATAAAAGA
    GCCCATGGTC AAGACTTGGG GACAGCTGGC AGCTGCCTTC GTCGCTTCAG TACCATGCCT
    TTCATGTTCT GCAACATTAA CAACGTTTGT AACTTTGCTT CAAGAAATGA CTATTCTTAC
    TGGCTTTCCA CCCCAGAGCC CATGCCAATG AACATGGAAC CCCTGAAGGG CCAGAGCATC
    CAGCCATTCA TTAGTCGATG TGCAGTATGT GAGGCACCAG CTGTGGTGAT TGCAGTTCAC
    AGCCAGACTA TTCAGATTCC TCACTGTCCT CAGGGGTGGG ACTCTCTGTG GATTGGCTAT
    TCATTCATGA TGCATACAAG TGCAGGAGCA GAAGGCTCTG GCCAAGCCCT AGCCTCTCCT
    GGCTCCTGTT TGGAAGAATT TCGTTCAGCT CCCTTCATCG AATGTCATGG ACGAGGGACA
    TGCAATTACT ATGCCAATTC TTACAGCTTT TGGCTGGCCA CTGTTGATAT GTCAGACATG
    TTCAGTAAAC CTCAGTCAGA GACACTGAAA GCAGGCGACT TGAGGACACG TATTAGCCGA
    TGTCAAGTAT GCATGAAAAG GACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa21618
    Clone ID Related Accession (Same CDS sequence) XM_006227490.1 , XM_006257312.1
    Accession Version XM_006257312.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5067bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product collagen alpha-5(IV) chain isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCAAGTGC CTGGAGTGTG CCTGGCCGCC GGCTGGTTCT TACTGGCCCT GTGTCTGTGG 
    GGGCAGCCGG GAGAGGCTGC GGCCTGCCAT GGGTGTTCTT CTGGATCAAA ATGTGACTGC
    AGTGGTATAA AAGGAGAAAA GGGAGAACGG GGCTTTCCAG GCTTAGAAGG CCATCCAGGT
    TTACCAGGAT TTCCAGGTCC AGAAGGGCCT CCAGGACCTC GGGGACAAAA GGGTGATGAT
    GGAATTCCAG GGCCACCAGG ACCAAAAGGA ATCAGAGGTC CTCCTGGACT TCCTGGATTT
    CCAGGGACAC CAGGCCTTCC TGGGATGCCA GGCCACGATG GGGCTCCAGG ACCTCAAGGT
    ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGCGGATTTC CAGGCAGTCC AGGCTTTCCC
    GGTTTACAAG GACCTCCAGG ACCACCTGGG ATCCCAGGTA TGAAGGGGGA GCCAGGCAGT
    ATAATCATGT CATCTCTGCC AGGGCCAAAG GGTAATCAAG GATACCCAGG TCCTCCTGGA
    ATACAAGGTC CACCTGGTCC CAAGGGTGTA TCAGGGCCAA GTGGTCCTCC AGGACCACCA
    GGTTTAATGG GCCCTCCTGG CCCACCAGGC CCACCGGGAC CAAAGGGGAA TATGGGCTTA
    AATTTCCAGG GACCCAAAGG CGAAAAAGGT GAACAAGGTC TTCAGGGTCC ACCTGGTCCA
    CCTGGGCAGA TCAGTGAACA GAAAAGACCA ATTGACCTAG ATTTTCAGAA AGGCGATCAG
    GGAGTTCCCG GTGATAGAGG GCCTCCTGGA CCTCCGGGAA TACCTGGTCC TCCCGGTCCT
    CCTGGTGGTG TGAAAGGTGA AAAGGGTGAG CCCGGAGAGC CAGGCAAAAG AGGTAAACCA
    GGCAAAGATG GAGAGACTGG TCAACCAGGA ATCCCAGGTT TGCCTGGTGA TCCTGGTTAC
    CCTGGTGAAC CAGGAAGGCA TGGTGAAAAG GGCCAAAAGG GTGATACTGG CCCACCTGGA
    CCACCTGGAT TTGTAATTCC TACACCTGGA ACTGGTGTGA CTATTGGAGC AAAAGGAAAT
    ATTGGGCAAC CTGGTTTGCC TGGAGAAAAA GGAGAGCGAG GATTTCCTGG CATACAAGGT
    CCACCTGGCT TTCCTGGACC TCCAGGGACA GCTGTCATGG GTCCCCCTGG TCCTCCTGGA
    TATCCTGGTG AAAGGGGCCA GAAAGGTGAT GAAGGTCCCC CTGGAATTTG TATTCCTGGA
    TCTCCTGGAC TTGATGGACA GCCTGGAGCT CCTGGCTTTC CAGGACCTCC TGGTCCTCCT
    GGCCCCCAGT TACCCCCGAG TGATGAAATC TGTAAAGCAG GCCCTCCAGG GCCCCCAGGA
    TCTCCAGGTG ACCAAGGACT CCAAGGAGAG CAAGGAGTAA AAGGTGACAA AGGTGATACT
    TGCTTCAACT GTATTGGAAC TGGCATTTCA GGGCCTCCAG GCCAACCAGG TTTACCAGGT
    CTCCCAGGTC CTCCAGGATC TCTTGGAATC CCTGGAGAGA AGGGCGAGAA AGGACAAGCT
    GGGACAACTG GTCCAAAAGG ATTGCCAGGC ATTCCTGGAC CTCCAGGTGC TCCAGGCTTT
    CCAGGGTCTA AGGGTGACCC TGGTGATGTT CTCACTCTTC CGGGAATGAA AGGTGAAAAA
    GGAGAATTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTC TACCTGGTAG TCCTGGAAAG
    GATGGTTTGC CAGGACTTCC TGGCCCCAAA GGAGAGCCCG GTGGAATCAC TTTTAAGGGT
    GAAAGGGGTC CTCCTGGGAA CCCAGGTTTA CCAGGTCTCC CAGGAAATAT GGGACCTACA
    GGCCCTGTTG GTTTTGGGCC TCCAGGCCCC GTAGGTGAAA AAGGCATACA AGGTGTGGCG
    GGAAATCCAG GCCAGCCAGG ATTACCAGGT CCTAAAGGTG ATCCAGGCCA GACCATCAGC
    CAGCCAGGAA AGCCTGGCTT GCCAGGGAAT CCAGGCAGAG ATGGTGAAGT GGGTCTTCCA
    GGTGATCCTG GACTTCCAGG CCAACCAGGC CTACCAGGAA TACCTGGTAG CAAAGGAGAA
    CCAGGTATCC CTGGGATTGG GCCTCCAGGA CCACCTGGTC CCAAAGGTTT TCCAGGAATT
    CCTGGACCTC CAGGACCTCC TGGGGCCCCT GGAAGAATGG GCCCAGAAGG GCCTCCTGGG
    TTACCGGGCT TTCCAGGACC CAAGGGAGAG CCAGGATTTG GGTTACCTGG GCCACCTGGG
    CCACCAGGAT TCCCAGGTTT CAAAGGAACA CCTGGTCCAA AAGGTGATCG TGGTTTACCA
    GGACCTCCAG GTCCTCCAGG ACACACTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAT
    GCTGGACCAA ATGGACAACC TGGCCCAATA GGGCCACCAG GGCTGCCAGG AATTGGTCTT
    CAGGGACCAC CAGGACCACC AGGGACTCCA GGGCCAATAG GCCAACCTGG CTTACATGGA
    ATACCAGGAG AGAAGGGAGA TCCAGGACCT CCTGGGCTTG ATGTTCCAGG ACTCCCTGGA
    GAGAGAGGTA GTCCAGGGCT CCCTGGAGCC CCTGGTCTTA TAGGACCTCC AGGATCACCT
    GGTATCCCTG GAAGAGCAGG TGTCCCTGGA TTTCCAGGTG TCAAAGGCGA AATGGGTATG
    ATGGGACCTC CAGGCCCACC GGGACCTTTG GGTACTCCTG GCAGGAGTGG TGCTCCTGGT
    CTTAAAGGTG ATGACGGCAT GCAAGGTCAG CCAGGTCTCC CTGGCCCTGT AGGAGAAAAG
    GGTGGTAAGG GAGAACCTGG CCTTCCAGGT CCTCCTGGAC CAATGCGTCC AGATTTCCTG
    GGATCAAAAG GAGAAAAGGG TGATCCTGGC TTACCAGGTA TTCCTGGACT TTCCGGACCA
    AAAGGCTACC AGGGATTACC TGGAGACCCA GGGCAACCTG GGCTGAGTGG ACAGCCGGGA
    TCACCAGGAC TACCAGGTCC CAAAGGTACT CCTGGTCTCC CTGGTCCACC AGGACTTACC
    GGACCTCCTG GACCTAAAGG AAACATTGGC GACATGGGTT TTCCTGGCCC TCAAGGTGTA
    GATGGGCCTC CTGGACCTCC TGGAGGTCCT GGACAACCTG GCTCTCCCGG GTTGCCTGGC
    CAGAAAGGAA GTAAAGGAGA ACCTGGAGTT TCAGGCATTG GTCTCCCTGG TCTTCCTGGT
    CCAAAGGGTG AACCTGGTCT GCCTGGATAT CCAGGAAACC CAGGTATCAA AGGTTCTATG
    GGCGAGGCTG GTCTGCCTGG CTTACCAGGA AGCCCTGGAG CAAAAGGTCA ACCCGGCCTG
    CCCGGATTCC CAGGAACACC AGGGCTTCCT GGACCAAAGG GTATGAATGG TCCTCCTGGT
    AACCCTGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGGCGTCC TGGACCACCA
    GGACCTCCTG GTGAAAAAGG CAAGCCAGGT CAAGATGGCA TTCCTGGACC AGCAGGACAA
    AAGGGAGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CTCCTGGACT CCCAGGACTT
    TCTGGTCAAA AGGGTGATGG AGGAGCCCCT GGCCTTCCAG GAAATCCTGG CCTTCCAGGT
    TCAAAGGGTG AACCAGGCTT TCAGGGATTC CCTGGTATGC CAGGTCCCCC AGGTCCCCCT
    GGTTCTCCTG GCCCATCTTT GGAAGGTCCC AAAGGAAACC CTGGGCCTCA AGGCCCTCCT
    GGGAGACCAG GTCCTCCAGG TCCACCTGGT ACAGAAGGTC CTCGTGGCCC CCCTGGAAAT
    GGAGGTATTA AAGGAGAGAG GGGAAATCCA GGTCCACCTG GGCAACCAGG CTTACCTGGC
    TTGAAAGGAG ATCAAGGACC ACCAGGCCTT CCGGGTAACC CTGGCCGACC AGGTCTTAAT
    GGAATGAAAG GAGATCCTGG TCTCCCTGGT GTTCCAGGAT TCCCAGGAAT GAAAGGACCC
    ATTGGAGTAC CAGGCTCAAC TGGCCCTGAT GGGGAACCAG GTCTTACGGG GCCCCCAGGT
    CCTCCTGGGT TGCCTGGTCC TTCAGGACAG AGTATTGTAA TCAAAGGAGA TCCTGGACCT
    CCAGGAATTC CTGGACAACC TGGATTAAAA GGTCTACCAG GACTTCCAGG CCCTCAAGGT
    CTACCAGGTC CAATTGGCCC TCCAGGAGAT CCAGGACGCA ATGGACTCCC AGGCTTTGAT
    GGTGCAGGAG GGCGCAAAGG AGACCCAGGC CTGCCGGGGC AGCCAGGTAG CCGTGGATTG
    GATGGTCCCC CAGGACCAGA TGGATTGCAA GGACCTCCAG GGCCCCCGGG AACCACCTCT
    GTTGCCCATG GATTCCTCAT CACACGTCAC AGTCAGACAA CAGATGCACC ACAATGCCCA
    CAGGGAACAG TCCATATCTA TGATGGCTTT TCTCTCTTAT ATGTACAAGG AAATAAAAGA
    GCCCATGGTC AAGACTTGGG GACAGCTGGC AGCTGCCTTC GTCGCTTCAG TACCATGCCT
    TTCATGTTCT GCAACATTAA CAACGTTTGT AACTTTGCTT CAAGAAATGA CTATTCTTAC
    TGGCTTTCCA CCCCAGAGCC CATGCCAATG AACATGGAAC CCCTGAAGGG CCAGAGCATC
    CAGCCATTCA TTAGTCGATG TGCAGTATGT GAGGCACCAG CTGTGGTGAT TGCAGTTCAC
    AGCCAGACTA TTCAGATTCC TCACTGTCCT CAGGGGTGGG ACTCTCTGTG GATTGGCTAT
    TCATTCATGA TGCATACAAG TGCAGGAGCA GAAGGCTCTG GCCAAGCCCT AGCCTCTCCT
    GGCTCCTGTT TGGAAGAATT TCGTTCAGCT CCCTTCATCG AATGTCATGG ACGAGGGACA
    TGCAATTACT ATGCCAATTC TTACAGCTTT TGGCTGGCCA CTGTTGATAT GTCAGACATG
    TTCAGTAAAC CTCAGTCAGA GACACTGAAA GCAGGCGACT TGAGGACACG TATTAGCCGA
    TGTCAAGTAT GCATGAAAAG GACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006257374.1
    CDS400..5466
    Translation

    Target ORF information:

    RefSeq Version XM_006257312.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus collagen type IV alpha 5 chain (Col4a5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006257312.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCAAGTGC CTGGAGTGTG CCTGGCCGCC GGCTGGTTCT TACTGGCCCT GTGTCTGTGG 
    GGGCAGCCGG GAGAGGCTGC GGCCTGCCAT GGGTGTTCTT CTGGATCAAA ATGTGACTGC
    AGTGGTATAA AAGGAGAAAA GGGAGAACGG GGCTTTCCAG GCTTAGAAGG CCATCCAGGT
    TTACCAGGAT TTCCAGGTCC AGAAGGGCCT CCAGGACCTC GGGGACAAAA GGGTGATGAT
    GGAATTCCAG GGCCACCAGG ACCAAAAGGA ATCAGAGGTC CTCCTGGACT TCCTGGATTT
    CCAGGGACAC CAGGCCTTCC TGGGATGCCA GGCCACGATG GGGCTCCAGG ACCTCAAGGT
    ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGCGGATTTC CAGGCAGTCC AGGCTTTCCC
    GGTTTACAAG GACCTCCAGG ACCACCTGGG ATCCCAGGTA TGAAGGGGGA GCCAGGCAGT
    ATAATCATGT CATCTCTGCC AGGGCCAAAG GGTAATCAAG GATACCCAGG TCCTCCTGGA
    ATACAAGGTC CACCTGGTCC CAAGGGTGTA TCAGGGCCAA GTGGTCCTCC AGGACCACCA
    GGTTTAATGG GCCCTCCTGG CCCACCAGGC CCACCGGGAC CAAAGGGGAA TATGGGCTTA
    AATTTCCAGG GACCCAAAGG CGAAAAAGGT GAACAAGGTC TTCAGGGTCC ACCTGGTCCA
    CCTGGGCAGA TCAGTGAACA GAAAAGACCA ATTGACCTAG ATTTTCAGAA AGGCGATCAG
    GGAGTTCCCG GTGATAGAGG GCCTCCTGGA CCTCCGGGAA TACCTGGTCC TCCCGGTCCT
    CCTGGTGGTG TGAAAGGTGA AAAGGGTGAG CCCGGAGAGC CAGGCAAAAG AGGTAAACCA
    GGCAAAGATG GAGAGACTGG TCAACCAGGA ATCCCAGGTT TGCCTGGTGA TCCTGGTTAC
    CCTGGTGAAC CAGGAAGGCA TGGTGAAAAG GGCCAAAAGG GTGATACTGG CCCACCTGGA
    CCACCTGGAT TTGTAATTCC TACACCTGGA ACTGGTGTGA CTATTGGAGC AAAAGGAAAT
    ATTGGGCAAC CTGGTTTGCC TGGAGAAAAA GGAGAGCGAG GATTTCCTGG CATACAAGGT
    CCACCTGGCT TTCCTGGACC TCCAGGGACA GCTGTCATGG GTCCCCCTGG TCCTCCTGGA
    TATCCTGGTG AAAGGGGCCA GAAAGGTGAT GAAGGTCCCC CTGGAATTTG TATTCCTGGA
    TCTCCTGGAC TTGATGGACA GCCTGGAGCT CCTGGCTTTC CAGGACCTCC TGGTCCTCCT
    GGCCCCCAGT TACCCCCGAG TGATGAAATC TGTAAAGCAG GCCCTCCAGG GCCCCCAGGA
    TCTCCAGGTG ACCAAGGACT CCAAGGAGAG CAAGGAGTAA AAGGTGACAA AGGTGATACT
    TGCTTCAACT GTATTGGAAC TGGCATTTCA GGGCCTCCAG GCCAACCAGG TTTACCAGGT
    CTCCCAGGTC CTCCAGGATC TCTTGGAATC CCTGGAGAGA AGGGCGAGAA AGGACAAGCT
    GGGACAACTG GTCCAAAAGG ATTGCCAGGC ATTCCTGGAC CTCCAGGTGC TCCAGGCTTT
    CCAGGGTCTA AGGGTGACCC TGGTGATGTT CTCACTCTTC CGGGAATGAA AGGTGAAAAA
    GGAGAATTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTC TACCTGGTAG TCCTGGAAAG
    GATGGTTTGC CAGGACTTCC TGGCCCCAAA GGAGAGCCCG GTGGAATCAC TTTTAAGGGT
    GAAAGGGGTC CTCCTGGGAA CCCAGGTTTA CCAGGTCTCC CAGGAAATAT GGGACCTACA
    GGCCCTGTTG GTTTTGGGCC TCCAGGCCCC GTAGGTGAAA AAGGCATACA AGGTGTGGCG
    GGAAATCCAG GCCAGCCAGG ATTACCAGGT CCTAAAGGTG ATCCAGGCCA GACCATCAGC
    CAGCCAGGAA AGCCTGGCTT GCCAGGGAAT CCAGGCAGAG ATGGTGAAGT GGGTCTTCCA
    GGTGATCCTG GACTTCCAGG CCAACCAGGC CTACCAGGAA TACCTGGTAG CAAAGGAGAA
    CCAGGTATCC CTGGGATTGG GCCTCCAGGA CCACCTGGTC CCAAAGGTTT TCCAGGAATT
    CCTGGACCTC CAGGACCTCC TGGGGCCCCT GGAAGAATGG GCCCAGAAGG GCCTCCTGGG
    TTACCGGGCT TTCCAGGACC CAAGGGAGAG CCAGGATTTG GGTTACCTGG GCCACCTGGG
    CCACCAGGAT TCCCAGGTTT CAAAGGAACA CCTGGTCCAA AAGGTGATCG TGGTTTACCA
    GGACCTCCAG GTCCTCCAGG ACACACTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAT
    GCTGGACCAA ATGGACAACC TGGCCCAATA GGGCCACCAG GGCTGCCAGG AATTGGTCTT
    CAGGGACCAC CAGGACCACC AGGGACTCCA GGGCCAATAG GCCAACCTGG CTTACATGGA
    ATACCAGGAG AGAAGGGAGA TCCAGGACCT CCTGGGCTTG ATGTTCCAGG ACTCCCTGGA
    GAGAGAGGTA GTCCAGGGCT CCCTGGAGCC CCTGGTCTTA TAGGACCTCC AGGATCACCT
    GGTATCCCTG GAAGAGCAGG TGTCCCTGGA TTTCCAGGTG TCAAAGGCGA AATGGGTATG
    ATGGGACCTC CAGGCCCACC GGGACCTTTG GGTACTCCTG GCAGGAGTGG TGCTCCTGGT
    CTTAAAGGTG ATGACGGCAT GCAAGGTCAG CCAGGTCTCC CTGGCCCTGT AGGAGAAAAG
    GGTGGTAAGG GAGAACCTGG CCTTCCAGGT CCTCCTGGAC CAATGCGTCC AGATTTCCTG
    GGATCAAAAG GAGAAAAGGG TGATCCTGGC TTACCAGGTA TTCCTGGACT TTCCGGACCA
    AAAGGCTACC AGGGATTACC TGGAGACCCA GGGCAACCTG GGCTGAGTGG ACAGCCGGGA
    TCACCAGGAC TACCAGGTCC CAAAGGTACT CCTGGTCTCC CTGGTCCACC AGGACTTACC
    GGACCTCCTG GACCTAAAGG AAACATTGGC GACATGGGTT TTCCTGGCCC TCAAGGTGTA
    GATGGGCCTC CTGGACCTCC TGGAGGTCCT GGACAACCTG GCTCTCCCGG GTTGCCTGGC
    CAGAAAGGAA GTAAAGGAGA ACCTGGAGTT TCAGGCATTG GTCTCCCTGG TCTTCCTGGT
    CCAAAGGGTG AACCTGGTCT GCCTGGATAT CCAGGAAACC CAGGTATCAA AGGTTCTATG
    GGCGAGGCTG GTCTGCCTGG CTTACCAGGA AGCCCTGGAG CAAAAGGTCA ACCCGGCCTG
    CCCGGATTCC CAGGAACACC AGGGCTTCCT GGACCAAAGG GTATGAATGG TCCTCCTGGT
    AACCCTGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGGCGTCC TGGACCACCA
    GGACCTCCTG GTGAAAAAGG CAAGCCAGGT CAAGATGGCA TTCCTGGACC AGCAGGACAA
    AAGGGAGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CTCCTGGACT CCCAGGACTT
    TCTGGTCAAA AGGGTGATGG AGGAGCCCCT GGCCTTCCAG GAAATCCTGG CCTTCCAGGT
    TCAAAGGGTG AACCAGGCTT TCAGGGATTC CCTGGTATGC CAGGTCCCCC AGGTCCCCCT
    GGTTCTCCTG GCCCATCTTT GGAAGGTCCC AAAGGAAACC CTGGGCCTCA AGGCCCTCCT
    GGGAGACCAG GTCCTCCAGG TCCACCTGGT ACAGAAGGTC CTCGTGGCCC CCCTGGAAAT
    GGAGGTATTA AAGGAGAGAG GGGAAATCCA GGTCCACCTG GGCAACCAGG CTTACCTGGC
    TTGAAAGGAG ATCAAGGACC ACCAGGCCTT CCGGGTAACC CTGGCCGACC AGGTCTTAAT
    GGAATGAAAG GAGATCCTGG TCTCCCTGGT GTTCCAGGAT TCCCAGGAAT GAAAGGACCC
    ATTGGAGTAC CAGGCTCAAC TGGCCCTGAT GGGGAACCAG GTCTTACGGG GCCCCCAGGT
    CCTCCTGGGT TGCCTGGTCC TTCAGGACAG AGTATTGTAA TCAAAGGAGA TCCTGGACCT
    CCAGGAATTC CTGGACAACC TGGATTAAAA GGTCTACCAG GACTTCCAGG CCCTCAAGGT
    CTACCAGGTC CAATTGGCCC TCCAGGAGAT CCAGGACGCA ATGGACTCCC AGGCTTTGAT
    GGTGCAGGAG GGCGCAAAGG AGACCCAGGC CTGCCGGGGC AGCCAGGTAG CCGTGGATTG
    GATGGTCCCC CAGGACCAGA TGGATTGCAA GGACCTCCAG GGCCCCCGGG AACCACCTCT
    GTTGCCCATG GATTCCTCAT CACACGTCAC AGTCAGACAA CAGATGCACC ACAATGCCCA
    CAGGGAACAG TCCATATCTA TGATGGCTTT TCTCTCTTAT ATGTACAAGG AAATAAAAGA
    GCCCATGGTC AAGACTTGGG GACAGCTGGC AGCTGCCTTC GTCGCTTCAG TACCATGCCT
    TTCATGTTCT GCAACATTAA CAACGTTTGT AACTTTGCTT CAAGAAATGA CTATTCTTAC
    TGGCTTTCCA CCCCAGAGCC CATGCCAATG AACATGGAAC CCCTGAAGGG CCAGAGCATC
    CAGCCATTCA TTAGTCGATG TGCAGTATGT GAGGCACCAG CTGTGGTGAT TGCAGTTCAC
    AGCCAGACTA TTCAGATTCC TCACTGTCCT CAGGGGTGGG ACTCTCTGTG GATTGGCTAT
    TCATTCATGA TGCATACAAG TGCAGGAGCA GAAGGCTCTG GCCAAGCCCT AGCCTCTCCT
    GGCTCCTGTT TGGAAGAATT TCGTTCAGCT CCCTTCATCG AATGTCATGG ACGAGGGACA
    TGCAATTACT ATGCCAATTC TTACAGCTTT TGGCTGGCCA CTGTTGATAT GTCAGACATG
    TTCAGTAAAC CTCAGTCAGA GACACTGAAA GCAGGCGACT TGAGGACACG TATTAGCCGA
    TGTCAAGTAT GCATGAAAAG GACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa21619
    Clone ID Related Accession (Same CDS sequence) XM_006257313.1 , XM_006227491.1
    Accession Version XM_006257313.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5058bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product collagen alpha-5(IV) chain isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCAAGTGC CTGGAGTGTG CCTGGCCGCC GGCTGGTTCT TACTGGCCCT GTGTCTGTGG 
    GGGCAGCCGG GAGAGGCTGC GGCCTGCCAT GGGTGTTCTT CTGGATCAAA ATGTGACTGC
    AGTGGTATAA AAGGAGAAAA GGGAGAACGG GGCTTTCCAG GCTTAGAAGG CCATCCAGGT
    TTACCAGGAT TTCCAGGTCC AGAAGGGCCT CCAGGACCTC GGGGACAAAA GGGTGATGAT
    GGAATTCCAG GGCCACCAGG ACCAAAAGGA ATCAGAGGTC CTCCTGGACT TCCTGGATTT
    CCAGGGACAC CAGGCCTTCC TGGGATGCCA GGCCACGATG GGGCTCCAGG ACCTCAAGGT
    ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGCGGATTTC CAGGCAGTCC AGGCTTTCCC
    GGTTTACAAG GACCTCCAGG ACCACCTGGG ATCCCAGGTA TGAAGGGGGA GCCAGGCAGT
    ATAATCATGT CATCTCTGCC AGGGCCAAAG GGTAATCAAG GATACCCAGG TCCTCCTGGA
    ATACAAGGTC CACCTGGTCC CAAGGGTGTA TCAGGGCCAA GTGGTCCTCC AGGACCACCA
    GGTTTAATGG GCCCTCCTGG CCCACCAGGC CCACCGGGAC CAAAGGGGAA TATGGGCTTA
    AATTTCCAGG GACCCAAAGG CGAAAAAGGT GAACAAGGTC TTCAGGGTCC ACCTGGTCCA
    CCTGGGCAGA TCAGTGAACA GAAAAGACCA ATTGACCTAG ATTTTCAGAA AGGCGATCAG
    GGAGTTCCCG GTGATAGAGG GCCTCCTGGA CCTCCGGGAA TACCTGGTCC TCCCGGTCCT
    CCTGGTGGTG TGAAAGGTGA AAAGGGTGAG CCCGGAGAGC CAGGCAAAAG AGGTAAACCA
    GGCAAAGATG GAGAGACTGG TCAACCAGGA ATCCCAGGTT TGCCTGGTGA TCCTGGTTAC
    CCTGGTGAAC CAGGAAGGCA TGGTGAAAAG GGCCAAAAGG GTGATACTGG CCCACCTGGA
    CCACCTGGAT TTGTAATTCC TACACCTGGA ACTGGTGTGA CTATTGGAGC AAAAGGAAAT
    ATTGGGCAAC CTGGTTTGCC TGGAGAAAAA GGAGAGCGAG GATTTCCTGG CATACAAGGT
    CCACCTGGCT TTCCTGGACC TCCAGGGACA GCTGTCATGG GTCCCCCTGG TCCTCCTGGA
    TATCCTGGTG AAAGGGGCCA GAAAGGTGAT GAAGGTCCCC CTGGAATTTG TATTCCTGGA
    TCTCCTGGAC TTGATGGACA GCCTGGAGCT CCTGGCTTTC CAGGACCTCC TGGTCCTCCT
    GGCCCCCAGT TACCCCCGAG TGATGAAATC TGTAAAGCAG GCCCTCCAGG GCCCCCAGGA
    TCTCCAGGTG ACCAAGGACT CCAAGGAGAG CAAGGAGTAA AAGGTGACAA AGGTGATACT
    TGCTTCAACT GTATTGGAAC TGGCATTTCA GGGCCTCCAG GCCAACCAGG TTTACCAGGT
    CTCCCAGGTC CTCCAGGATC TCTTGGAATC CCTGGAGAGA AGGGCGAGAA AGGACAAGCT
    GGGACAACTG GTCCAAAAGG ATTGCCAGGC ATTCCTGGAC CTCCAGGTGC TCCAGGCTTT
    CCAGGGTCTA AGGGTGACCC TGGTGATGTT CTCACTCTTC CGGGAATGAA AGGTGAAAAA
    GGAGAATTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTC TACCTGGTAG TCCTGGAAAG
    GATGGTTTGC CAGGACTTCC TGGCCCCAAA GGAGAGCCCG GTGGAATCAC TTTTAAGGGT
    GAAAGGGGTC CTCCTGGGAA CCCAGGTTTA CCAGGTCTCC CAGGAAATAT GGGACCTACA
    GGCCCTGTTG GTTTTGGGCC TCCAGGCCCC GTAGGTGAAA AAGGCATACA AGGTGTGGCG
    GGAAATCCAG GCCAGCCAGG ATTACCAGGT CCTAAAGGTG ATCCAGGCCA GACCATCAGC
    CAGCCAGGAA AGCCTGGCTT GCCAGGGAAT CCAGGCAGAG ATGGTGAAGT GGGTCTTCCA
    GGTGATCCTG GACTTCCAGG CCAACCAGGC CTACCAGGAA TACCTGGTAG CAAAGGAGAA
    CCAGGTATCC CTGGGATTGG GCCTCCAGGA CCACCTGGTC CCAAAGGTTT TCCAGGAATT
    CCTGGACCTC CAGGACCTCC TGGGGCCCCT GGAAGAATGG GCCCAGAAGG GCCTCCTGGG
    TTACCGGGCT TTCCAGGACC CAAGGGAGAG CCAGGATTTG GGTTACCTGG GCCACCTGGG
    CCACCAGGAT TCCCAGGTTT CAAAGGAACA CCTGGTCCAA AAGGTGATCG TGGTTTACCA
    GGACCTCCAG GTCCTCCAGG ACACACTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAT
    GCTGGACCAA ATGGACAACC TGGCCCAATA GGGCCACCAG GGCTGCCAGG AATTGGTCTT
    CAGGGACCAC CAGGACCACC AGGGACTCCA GGGCCAATAG GCCAACCTGG CTTACATGGA
    ATACCAGGAG AGAAGGGAGA TCCAGGACCT CCTGGGCTTG ATGTTCCAGG ACTCCCTGGA
    GAGAGAGGTA GTCCAGGGCT CCCTGGAGCC CCTGGTCTTA TAGGACCTCC AGGATCACCT
    GGTATCCCTG GAAGAGCAGG TGTCCCTGGA TTTCCAGGTG TCAAAGGCGA AATGGGTATG
    ATGGGACCTC CAGGCCCACC GGGACCTTTG GGTACTCCTG GCAGGAGTGG TGCTCCTGGT
    CTTAAAGGTG ATGACGGCAT GCAAGGTCAG CCAGGTCTCC CTGGCCCTGT AGGAGAAAAG
    GGTGGTAAGG GAGAACCTGG CCTTCCAGGT CCTCCTGGAC CAATGCGTCC AGATTTCCTG
    GGATCAAAAG GAGAAAAGGG TGATCCTGGC TTACCAGGTA TTCCTGGACT TTCCGGACCA
    AAAGGCTACC AGGGATTACC TGGAGACCCA GGGCAACCTG GGCTGAGTGG ACAGCCGGGA
    TCACCAGGAC TACCAGGTCC CAAAGGTACT CCTGGTCTCC CTGGTCCACC AGGACTTACC
    GGACCTCCTG GACCTAAAGG AAACATTGGC GACATGGGTT TTCCTGGCCC TCAAGGTGTA
    GATGGGCCTC CTGGACCTCC TGGAGGTCCT GGACAACCTG GCTCTCCCGG GTTGCCTGGC
    CAGAAAGGAA GTAAAGGAGA ACCTGGAGTT TCAGGCATTG GTCTCCCTGG TCTTCCTGGT
    CCAAAGGGTG AACCTGGTCT GCCTGGATAT CCAGGAAACC CAGGTATCAA AGGTTCTATG
    GGCGAGGCTG GTCTGCCTGG CTTACCAGGA AGCCCTGGAG CAAAAGGTCA ACCCGGCCTG
    CCCGGATTCC CAGGAACACC AGGGCTTCCT GGACCAAAGG GTATGAATGG TCCTCCTGGT
    AACCCTGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGGCGTCC TGGACCACCA
    GGACCTCCTG GTGAAAAAGG CAAGCCAGGT CAAGATGGCA TTCCTGGACC AGCAGGACAA
    AAGGGAGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CTCCTGGACT CCCAGGACTT
    TCTGGTCAAA AGGGTGATGG AGGAGCCCCT GGCCTTCCAG GAAATCCTGG CCTTCCAGGT
    TCAAAGGGTG AACCAGGCTT TCAGGGATTC CCTGGTATGC CAGGTCCCCC AGGTCCCCCT
    GGTTCTCCTG GCCCATCTTT GGAAGGTCCC AAAGGAAACC CTGGGCCTCA AGGCCCTCCT
    GGGAGACCAG GTCCACCTGG TACAGAAGGT CCTCGTGGCC CCCCTGGAAA TGGAGGTATT
    AAAGGAGAGA GGGGAAATCC AGGTCCACCT GGGCAACCAG GCTTACCTGG CTTGAAAGGA
    GATCAAGGAC CACCAGGCCT TCCGGGTAAC CCTGGCCGAC CAGGTCTTAA TGGAATGAAA
    GGAGATCCTG GTCTCCCTGG TGTTCCAGGA TTCCCAGGAA TGAAAGGACC CATTGGAGTA
    CCAGGCTCAA CTGGCCCTGA TGGGGAACCA GGTCTTACGG GGCCCCCAGG TCCTCCTGGG
    TTGCCTGGTC CTTCAGGACA GAGTATTGTA ATCAAAGGAG ATCCTGGACC TCCAGGAATT
    CCTGGACAAC CTGGATTAAA AGGTCTACCA GGACTTCCAG GCCCTCAAGG TCTACCAGGT
    CCAATTGGCC CTCCAGGAGA TCCAGGACGC AATGGACTCC CAGGCTTTGA TGGTGCAGGA
    GGGCGCAAAG GAGACCCAGG CCTGCCGGGG CAGCCAGGTA GCCGTGGATT GGATGGTCCC
    CCAGGACCAG ATGGATTGCA AGGACCTCCA GGGCCCCCGG GAACCACCTC TGTTGCCCAT
    GGATTCCTCA TCACACGTCA CAGTCAGACA ACAGATGCAC CACAATGCCC ACAGGGAACA
    GTCCATATCT ATGATGGCTT TTCTCTCTTA TATGTACAAG GAAATAAAAG AGCCCATGGT
    CAAGACTTGG GGACAGCTGG CAGCTGCCTT CGTCGCTTCA GTACCATGCC TTTCATGTTC
    TGCAACATTA ACAACGTTTG TAACTTTGCT TCAAGAAATG ACTATTCTTA CTGGCTTTCC
    ACCCCAGAGC CCATGCCAAT GAACATGGAA CCCCTGAAGG GCCAGAGCAT CCAGCCATTC
    ATTAGTCGAT GTGCAGTATG TGAGGCACCA GCTGTGGTGA TTGCAGTTCA CAGCCAGACT
    ATTCAGATTC CTCACTGTCC TCAGGGGTGG GACTCTCTGT GGATTGGCTA TTCATTCATG
    ATGCATACAA GTGCAGGAGC AGAAGGCTCT GGCCAAGCCC TAGCCTCTCC TGGCTCCTGT
    TTGGAAGAAT TTCGTTCAGC TCCCTTCATC GAATGTCATG GACGAGGGAC ATGCAATTAC
    TATGCCAATT CTTACAGCTT TTGGCTGGCC ACTGTTGATA TGTCAGACAT GTTCAGTAAA
    CCTCAGTCAG AGACACTGAA AGCAGGCGAC TTGAGGACAC GTATTAGCCG ATGTCAAGTA
    TGCATGAAAA GGACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006257375.1
    CDS400..5457
    Translation

    Target ORF information:

    RefSeq Version XM_006257313.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus collagen type IV alpha 5 chain (Col4a5), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006257313.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCAAGTGC CTGGAGTGTG CCTGGCCGCC GGCTGGTTCT TACTGGCCCT GTGTCTGTGG 
    GGGCAGCCGG GAGAGGCTGC GGCCTGCCAT GGGTGTTCTT CTGGATCAAA ATGTGACTGC
    AGTGGTATAA AAGGAGAAAA GGGAGAACGG GGCTTTCCAG GCTTAGAAGG CCATCCAGGT
    TTACCAGGAT TTCCAGGTCC AGAAGGGCCT CCAGGACCTC GGGGACAAAA GGGTGATGAT
    GGAATTCCAG GGCCACCAGG ACCAAAAGGA ATCAGAGGTC CTCCTGGACT TCCTGGATTT
    CCAGGGACAC CAGGCCTTCC TGGGATGCCA GGCCACGATG GGGCTCCAGG ACCTCAAGGT
    ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGCGGATTTC CAGGCAGTCC AGGCTTTCCC
    GGTTTACAAG GACCTCCAGG ACCACCTGGG ATCCCAGGTA TGAAGGGGGA GCCAGGCAGT
    ATAATCATGT CATCTCTGCC AGGGCCAAAG GGTAATCAAG GATACCCAGG TCCTCCTGGA
    ATACAAGGTC CACCTGGTCC CAAGGGTGTA TCAGGGCCAA GTGGTCCTCC AGGACCACCA
    GGTTTAATGG GCCCTCCTGG CCCACCAGGC CCACCGGGAC CAAAGGGGAA TATGGGCTTA
    AATTTCCAGG GACCCAAAGG CGAAAAAGGT GAACAAGGTC TTCAGGGTCC ACCTGGTCCA
    CCTGGGCAGA TCAGTGAACA GAAAAGACCA ATTGACCTAG ATTTTCAGAA AGGCGATCAG
    GGAGTTCCCG GTGATAGAGG GCCTCCTGGA CCTCCGGGAA TACCTGGTCC TCCCGGTCCT
    CCTGGTGGTG TGAAAGGTGA AAAGGGTGAG CCCGGAGAGC CAGGCAAAAG AGGTAAACCA
    GGCAAAGATG GAGAGACTGG TCAACCAGGA ATCCCAGGTT TGCCTGGTGA TCCTGGTTAC
    CCTGGTGAAC CAGGAAGGCA TGGTGAAAAG GGCCAAAAGG GTGATACTGG CCCACCTGGA
    CCACCTGGAT TTGTAATTCC TACACCTGGA ACTGGTGTGA CTATTGGAGC AAAAGGAAAT
    ATTGGGCAAC CTGGTTTGCC TGGAGAAAAA GGAGAGCGAG GATTTCCTGG CATACAAGGT
    CCACCTGGCT TTCCTGGACC TCCAGGGACA GCTGTCATGG GTCCCCCTGG TCCTCCTGGA
    TATCCTGGTG AAAGGGGCCA GAAAGGTGAT GAAGGTCCCC CTGGAATTTG TATTCCTGGA
    TCTCCTGGAC TTGATGGACA GCCTGGAGCT CCTGGCTTTC CAGGACCTCC TGGTCCTCCT
    GGCCCCCAGT TACCCCCGAG TGATGAAATC TGTAAAGCAG GCCCTCCAGG GCCCCCAGGA
    TCTCCAGGTG ACCAAGGACT CCAAGGAGAG CAAGGAGTAA AAGGTGACAA AGGTGATACT
    TGCTTCAACT GTATTGGAAC TGGCATTTCA GGGCCTCCAG GCCAACCAGG TTTACCAGGT
    CTCCCAGGTC CTCCAGGATC TCTTGGAATC CCTGGAGAGA AGGGCGAGAA AGGACAAGCT
    GGGACAACTG GTCCAAAAGG ATTGCCAGGC ATTCCTGGAC CTCCAGGTGC TCCAGGCTTT
    CCAGGGTCTA AGGGTGACCC TGGTGATGTT CTCACTCTTC CGGGAATGAA AGGTGAAAAA
    GGAGAATTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTC TACCTGGTAG TCCTGGAAAG
    GATGGTTTGC CAGGACTTCC TGGCCCCAAA GGAGAGCCCG GTGGAATCAC TTTTAAGGGT
    GAAAGGGGTC CTCCTGGGAA CCCAGGTTTA CCAGGTCTCC CAGGAAATAT GGGACCTACA
    GGCCCTGTTG GTTTTGGGCC TCCAGGCCCC GTAGGTGAAA AAGGCATACA AGGTGTGGCG
    GGAAATCCAG GCCAGCCAGG ATTACCAGGT CCTAAAGGTG ATCCAGGCCA GACCATCAGC
    CAGCCAGGAA AGCCTGGCTT GCCAGGGAAT CCAGGCAGAG ATGGTGAAGT GGGTCTTCCA
    GGTGATCCTG GACTTCCAGG CCAACCAGGC CTACCAGGAA TACCTGGTAG CAAAGGAGAA
    CCAGGTATCC CTGGGATTGG GCCTCCAGGA CCACCTGGTC CCAAAGGTTT TCCAGGAATT
    CCTGGACCTC CAGGACCTCC TGGGGCCCCT GGAAGAATGG GCCCAGAAGG GCCTCCTGGG
    TTACCGGGCT TTCCAGGACC CAAGGGAGAG CCAGGATTTG GGTTACCTGG GCCACCTGGG
    CCACCAGGAT TCCCAGGTTT CAAAGGAACA CCTGGTCCAA AAGGTGATCG TGGTTTACCA
    GGACCTCCAG GTCCTCCAGG ACACACTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAT
    GCTGGACCAA ATGGACAACC TGGCCCAATA GGGCCACCAG GGCTGCCAGG AATTGGTCTT
    CAGGGACCAC CAGGACCACC AGGGACTCCA GGGCCAATAG GCCAACCTGG CTTACATGGA
    ATACCAGGAG AGAAGGGAGA TCCAGGACCT CCTGGGCTTG ATGTTCCAGG ACTCCCTGGA
    GAGAGAGGTA GTCCAGGGCT CCCTGGAGCC CCTGGTCTTA TAGGACCTCC AGGATCACCT
    GGTATCCCTG GAAGAGCAGG TGTCCCTGGA TTTCCAGGTG TCAAAGGCGA AATGGGTATG
    ATGGGACCTC CAGGCCCACC GGGACCTTTG GGTACTCCTG GCAGGAGTGG TGCTCCTGGT
    CTTAAAGGTG ATGACGGCAT GCAAGGTCAG CCAGGTCTCC CTGGCCCTGT AGGAGAAAAG
    GGTGGTAAGG GAGAACCTGG CCTTCCAGGT CCTCCTGGAC CAATGCGTCC AGATTTCCTG
    GGATCAAAAG GAGAAAAGGG TGATCCTGGC TTACCAGGTA TTCCTGGACT TTCCGGACCA
    AAAGGCTACC AGGGATTACC TGGAGACCCA GGGCAACCTG GGCTGAGTGG ACAGCCGGGA
    TCACCAGGAC TACCAGGTCC CAAAGGTACT CCTGGTCTCC CTGGTCCACC AGGACTTACC
    GGACCTCCTG GACCTAAAGG AAACATTGGC GACATGGGTT TTCCTGGCCC TCAAGGTGTA
    GATGGGCCTC CTGGACCTCC TGGAGGTCCT GGACAACCTG GCTCTCCCGG GTTGCCTGGC
    CAGAAAGGAA GTAAAGGAGA ACCTGGAGTT TCAGGCATTG GTCTCCCTGG TCTTCCTGGT
    CCAAAGGGTG AACCTGGTCT GCCTGGATAT CCAGGAAACC CAGGTATCAA AGGTTCTATG
    GGCGAGGCTG GTCTGCCTGG CTTACCAGGA AGCCCTGGAG CAAAAGGTCA ACCCGGCCTG
    CCCGGATTCC CAGGAACACC AGGGCTTCCT GGACCAAAGG GTATGAATGG TCCTCCTGGT
    AACCCTGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGGCGTCC TGGACCACCA
    GGACCTCCTG GTGAAAAAGG CAAGCCAGGT CAAGATGGCA TTCCTGGACC AGCAGGACAA
    AAGGGAGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CTCCTGGACT CCCAGGACTT
    TCTGGTCAAA AGGGTGATGG AGGAGCCCCT GGCCTTCCAG GAAATCCTGG CCTTCCAGGT
    TCAAAGGGTG AACCAGGCTT TCAGGGATTC CCTGGTATGC CAGGTCCCCC AGGTCCCCCT
    GGTTCTCCTG GCCCATCTTT GGAAGGTCCC AAAGGAAACC CTGGGCCTCA AGGCCCTCCT
    GGGAGACCAG GTCCACCTGG TACAGAAGGT CCTCGTGGCC CCCCTGGAAA TGGAGGTATT
    AAAGGAGAGA GGGGAAATCC AGGTCCACCT GGGCAACCAG GCTTACCTGG CTTGAAAGGA
    GATCAAGGAC CACCAGGCCT TCCGGGTAAC CCTGGCCGAC CAGGTCTTAA TGGAATGAAA
    GGAGATCCTG GTCTCCCTGG TGTTCCAGGA TTCCCAGGAA TGAAAGGACC CATTGGAGTA
    CCAGGCTCAA CTGGCCCTGA TGGGGAACCA GGTCTTACGG GGCCCCCAGG TCCTCCTGGG
    TTGCCTGGTC CTTCAGGACA GAGTATTGTA ATCAAAGGAG ATCCTGGACC TCCAGGAATT
    CCTGGACAAC CTGGATTAAA AGGTCTACCA GGACTTCCAG GCCCTCAAGG TCTACCAGGT
    CCAATTGGCC CTCCAGGAGA TCCAGGACGC AATGGACTCC CAGGCTTTGA TGGTGCAGGA
    GGGCGCAAAG GAGACCCAGG CCTGCCGGGG CAGCCAGGTA GCCGTGGATT GGATGGTCCC
    CCAGGACCAG ATGGATTGCA AGGACCTCCA GGGCCCCCGG GAACCACCTC TGTTGCCCAT
    GGATTCCTCA TCACACGTCA CAGTCAGACA ACAGATGCAC CACAATGCCC ACAGGGAACA
    GTCCATATCT ATGATGGCTT TTCTCTCTTA TATGTACAAG GAAATAAAAG AGCCCATGGT
    CAAGACTTGG GGACAGCTGG CAGCTGCCTT CGTCGCTTCA GTACCATGCC TTTCATGTTC
    TGCAACATTA ACAACGTTTG TAACTTTGCT TCAAGAAATG ACTATTCTTA CTGGCTTTCC
    ACCCCAGAGC CCATGCCAAT GAACATGGAA CCCCTGAAGG GCCAGAGCAT CCAGCCATTC
    ATTAGTCGAT GTGCAGTATG TGAGGCACCA GCTGTGGTGA TTGCAGTTCA CAGCCAGACT
    ATTCAGATTC CTCACTGTCC TCAGGGGTGG GACTCTCTGT GGATTGGCTA TTCATTCATG
    ATGCATACAA GTGCAGGAGC AGAAGGCTCT GGCCAAGCCC TAGCCTCTCC TGGCTCCTGT
    TTGGAAGAAT TTCGTTCAGC TCCCTTCATC GAATGTCATG GACGAGGGAC ATGCAATTAC
    TATGCCAATT CTTACAGCTT TTGGCTGGCC ACTGTTGATA TGTCAGACAT GTTCAGTAAA
    CCTCAGTCAG AGACACTGAA AGCAGGCGAC TTGAGGACAC GTATTAGCCG ATGTCAAGTA
    TGCATGAAAA GGACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa21619
    Clone ID Related Accession (Same CDS sequence) XM_006257313.1 , XM_006227491.1
    Accession Version XM_006227491.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5058bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product collagen alpha-5(IV) chain isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000089.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCAAGTGC CTGGAGTGTG CCTGGCCGCC GGCTGGTTCT TACTGGCCCT GTGTCTGTGG 
    GGGCAGCCGG GAGAGGCTGC GGCCTGCCAT GGGTGTTCTT CTGGATCAAA ATGTGACTGC
    AGTGGTATAA AAGGAGAAAA GGGAGAACGG GGCTTTCCAG GCTTAGAAGG CCATCCAGGT
    TTACCAGGAT TTCCAGGTCC AGAAGGGCCT CCAGGACCTC GGGGACAAAA GGGTGATGAT
    GGAATTCCAG GGCCACCAGG ACCAAAAGGA ATCAGAGGTC CTCCTGGACT TCCTGGATTT
    CCAGGGACAC CAGGCCTTCC TGGGATGCCA GGCCACGATG GGGCTCCAGG ACCTCAAGGT
    ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGCGGATTTC CAGGCAGTCC AGGCTTTCCC
    GGTTTACAAG GACCTCCAGG ACCACCTGGG ATCCCAGGTA TGAAGGGGGA GCCAGGCAGT
    ATAATCATGT CATCTCTGCC AGGGCCAAAG GGTAATCAAG GATACCCAGG TCCTCCTGGA
    ATACAAGGTC CACCTGGTCC CAAGGGTGTA TCAGGGCCAA GTGGTCCTCC AGGACCACCA
    GGTTTAATGG GCCCTCCTGG CCCACCAGGC CCACCGGGAC CAAAGGGGAA TATGGGCTTA
    AATTTCCAGG GACCCAAAGG CGAAAAAGGT GAACAAGGTC TTCAGGGTCC ACCTGGTCCA
    CCTGGGCAGA TCAGTGAACA GAAAAGACCA ATTGACCTAG ATTTTCAGAA AGGCGATCAG
    GGAGTTCCCG GTGATAGAGG GCCTCCTGGA CCTCCGGGAA TACCTGGTCC TCCCGGTCCT
    CCTGGTGGTG TGAAAGGTGA AAAGGGTGAG CCCGGAGAGC CAGGCAAAAG AGGTAAACCA
    GGCAAAGATG GAGAGACTGG TCAACCAGGA ATCCCAGGTT TGCCTGGTGA TCCTGGTTAC
    CCTGGTGAAC CAGGAAGGCA TGGTGAAAAG GGCCAAAAGG GTGATACTGG CCCACCTGGA
    CCACCTGGAT TTGTAATTCC TACACCTGGA ACTGGTGTGA CTATTGGAGC AAAAGGAAAT
    ATTGGGCAAC CTGGTTTGCC TGGAGAAAAA GGAGAGCGAG GATTTCCTGG CATACAAGGT
    CCACCTGGCT TTCCTGGACC TCCAGGGACA GCTGTCATGG GTCCCCCTGG TCCTCCTGGA
    TATCCTGGTG AAAGGGGCCA GAAAGGTGAT GAAGGTCCCC CTGGAATTTG TATTCCTGGA
    TCTCCTGGAC TTGATGGACA GCCTGGAGCT CCTGGCTTTC CAGGACCTCC TGGTCCTCCT
    GGCCCCCAGT TACCCCCGAG TGATGAAATC TGTAAAGCAG GCCCTCCAGG GCCCCCAGGA
    TCTCCAGGTG ACCAAGGACT CCAAGGAGAG CAAGGAGTAA AAGGTGACAA AGGTGATACT
    TGCTTCAACT GTATTGGAAC TGGCATTTCA GGGCCTCCAG GCCAACCAGG TTTACCAGGT
    CTCCCAGGTC CTCCAGGATC TCTTGGAATC CCTGGAGAGA AGGGCGAGAA AGGACAAGCT
    GGGACAACTG GTCCAAAAGG ATTGCCAGGC ATTCCTGGAC CTCCAGGTGC TCCAGGCTTT
    CCAGGGTCTA AGGGTGACCC TGGTGATGTT CTCACTCTTC CGGGAATGAA AGGTGAAAAA
    GGAGAATTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTC TACCTGGTAG TCCTGGAAAG
    GATGGTTTGC CAGGACTTCC TGGCCCCAAA GGAGAGCCCG GTGGAATCAC TTTTAAGGGT
    GAAAGGGGTC CTCCTGGGAA CCCAGGTTTA CCAGGTCTCC CAGGAAATAT GGGACCTACA
    GGCCCTGTTG GTTTTGGGCC TCCAGGCCCC GTAGGTGAAA AAGGCATACA AGGTGTGGCG
    GGAAATCCAG GCCAGCCAGG ATTACCAGGT CCTAAAGGTG ATCCAGGCCA GACCATCAGC
    CAGCCAGGAA AGCCTGGCTT GCCAGGGAAT CCAGGCAGAG ATGGTGAAGT GGGTCTTCCA
    GGTGATCCTG GACTTCCAGG CCAACCAGGC CTACCAGGAA TACCTGGTAG CAAAGGAGAA
    CCAGGTATCC CTGGGATTGG GCCTCCAGGA CCACCTGGTC CCAAAGGTTT TCCAGGAATT
    CCTGGACCTC CAGGACCTCC TGGGGCCCCT GGAAGAATGG GCCCAGAAGG GCCTCCTGGG
    TTACCGGGCT TTCCAGGACC CAAGGGAGAG CCAGGATTTG GGTTACCTGG GCCACCTGGG
    CCACCAGGAT TCCCAGGTTT CAAAGGAACA CCTGGTCCAA AAGGTGATCG TGGTTTACCA
    GGACCTCCAG GTCCTCCAGG ACACACTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAT
    GCTGGACCAA ATGGACAACC TGGCCCAATA GGGCCACCAG GGCTGCCAGG AATTGGTCTT
    CAGGGACCAC CAGGACCACC AGGGACTCCA GGGCCAATAG GCCAACCTGG CTTACATGGA
    ATACCAGGAG AGAAGGGAGA TCCAGGACCT CCTGGGCTTG ATGTTCCAGG ACTCCCTGGA
    GAGAGAGGTA GTCCAGGGCT CCCTGGAGCC CCTGGTCTTA TAGGACCTCC AGGATCACCT
    GGTATCCCTG GAAGAGCAGG TGTCCCTGGA TTTCCAGGTG TCAAAGGCGA AATGGGTATG
    ATGGGACCTC CAGGCCCACC GGGACCTTTG GGTACTCCTG GCAGGAGTGG TGCTCCTGGT
    CTTAAAGGTG ATGACGGCAT GCAAGGTCAG CCAGGTCTCC CTGGCCCTGT AGGAGAAAAG
    GGTGGTAAGG GAGAACCTGG CCTTCCAGGT CCTCCTGGAC CAATGCGTCC AGATTTCCTG
    GGATCAAAAG GAGAAAAGGG TGATCCTGGC TTACCAGGTA TTCCTGGACT TTCCGGACCA
    AAAGGCTACC AGGGATTACC TGGAGACCCA GGGCAACCTG GGCTGAGTGG ACAGCCGGGA
    TCACCAGGAC TACCAGGTCC CAAAGGTACT CCTGGTCTCC CTGGTCCACC AGGACTTACC
    GGACCTCCTG GACCTAAAGG AAACATTGGC GACATGGGTT TTCCTGGCCC TCAAGGTGTA
    GATGGGCCTC CTGGACCTCC TGGAGGTCCT GGACAACCTG GCTCTCCCGG GTTGCCTGGC
    CAGAAAGGAA GTAAAGGAGA ACCTGGAGTT TCAGGCATTG GTCTCCCTGG TCTTCCTGGT
    CCAAAGGGTG AACCTGGTCT GCCTGGATAT CCAGGAAACC CAGGTATCAA AGGTTCTATG
    GGCGAGGCTG GTCTGCCTGG CTTACCAGGA AGCCCTGGAG CAAAAGGTCA ACCCGGCCTG
    CCCGGATTCC CAGGAACACC AGGGCTTCCT GGACCAAAGG GTATGAATGG TCCTCCTGGT
    AACCCTGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGGCGTCC TGGACCACCA
    GGACCTCCTG GTGAAAAAGG CAAGCCAGGT CAAGATGGCA TTCCTGGACC AGCAGGACAA
    AAGGGAGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CTCCTGGACT CCCAGGACTT
    TCTGGTCAAA AGGGTGATGG AGGAGCCCCT GGCCTTCCAG GAAATCCTGG CCTTCCAGGT
    TCAAAGGGTG AACCAGGCTT TCAGGGATTC CCTGGTATGC CAGGTCCCCC AGGTCCCCCT
    GGTTCTCCTG GCCCATCTTT GGAAGGTCCC AAAGGAAACC CTGGGCCTCA AGGCCCTCCT
    GGGAGACCAG GTCCACCTGG TACAGAAGGT CCTCGTGGCC CCCCTGGAAA TGGAGGTATT
    AAAGGAGAGA GGGGAAATCC AGGTCCACCT GGGCAACCAG GCTTACCTGG CTTGAAAGGA
    GATCAAGGAC CACCAGGCCT TCCGGGTAAC CCTGGCCGAC CAGGTCTTAA TGGAATGAAA
    GGAGATCCTG GTCTCCCTGG TGTTCCAGGA TTCCCAGGAA TGAAAGGACC CATTGGAGTA
    CCAGGCTCAA CTGGCCCTGA TGGGGAACCA GGTCTTACGG GGCCCCCAGG TCCTCCTGGG
    TTGCCTGGTC CTTCAGGACA GAGTATTGTA ATCAAAGGAG ATCCTGGACC TCCAGGAATT
    CCTGGACAAC CTGGATTAAA AGGTCTACCA GGACTTCCAG GCCCTCAAGG TCTACCAGGT
    CCAATTGGCC CTCCAGGAGA TCCAGGACGC AATGGACTCC CAGGCTTTGA TGGTGCAGGA
    GGGCGCAAAG GAGACCCAGG CCTGCCGGGG CAGCCAGGTA GCCGTGGATT GGATGGTCCC
    CCAGGACCAG ATGGATTGCA AGGACCTCCA GGGCCCCCGG GAACCACCTC TGTTGCCCAT
    GGATTCCTCA TCACACGTCA CAGTCAGACA ACAGATGCAC CACAATGCCC ACAGGGAACA
    GTCCATATCT ATGATGGCTT TTCTCTCTTA TATGTACAAG GAAATAAAAG AGCCCATGGT
    CAAGACTTGG GGACAGCTGG CAGCTGCCTT CGTCGCTTCA GTACCATGCC TTTCATGTTC
    TGCAACATTA ACAACGTTTG TAACTTTGCT TCAAGAAATG ACTATTCTTA CTGGCTTTCC
    ACCCCAGAGC CCATGCCAAT GAACATGGAA CCCCTGAAGG GCCAGAGCAT CCAGCCATTC
    ATTAGTCGAT GTGCAGTATG TGAGGCACCA GCTGTGGTGA TTGCAGTTCA CAGCCAGACT
    ATTCAGATTC CTCACTGTCC TCAGGGGTGG GACTCTCTGT GGATTGGCTA TTCATTCATG
    ATGCATACAA GTGCAGGAGC AGAAGGCTCT GGCCAAGCCC TAGCCTCTCC TGGCTCCTGT
    TTGGAAGAAT TTCGTTCAGC TCCCTTCATC GAATGTCATG GACGAGGGAC ATGCAATTAC
    TATGCCAATT CTTACAGCTT TTGGCTGGCC ACTGTTGATA TGTCAGACAT GTTCAGTAAA
    CCTCAGTCAG AGACACTGAA AGCAGGCGAC TTGAGGACAC GTATTAGCCG ATGTCAAGTA
    TGCATGAAAA GGACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006227553.1
    CDS400..5457
    Translation

    Target ORF information:

    RefSeq Version XM_006227491.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus collagen type IV alpha 5 chain (Col4a5), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006227491.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCAAGTGC CTGGAGTGTG CCTGGCCGCC GGCTGGTTCT TACTGGCCCT GTGTCTGTGG 
    GGGCAGCCGG GAGAGGCTGC GGCCTGCCAT GGGTGTTCTT CTGGATCAAA ATGTGACTGC
    AGTGGTATAA AAGGAGAAAA GGGAGAACGG GGCTTTCCAG GCTTAGAAGG CCATCCAGGT
    TTACCAGGAT TTCCAGGTCC AGAAGGGCCT CCAGGACCTC GGGGACAAAA GGGTGATGAT
    GGAATTCCAG GGCCACCAGG ACCAAAAGGA ATCAGAGGTC CTCCTGGACT TCCTGGATTT
    CCAGGGACAC CAGGCCTTCC TGGGATGCCA GGCCACGATG GGGCTCCAGG ACCTCAAGGT
    ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGCGGATTTC CAGGCAGTCC AGGCTTTCCC
    GGTTTACAAG GACCTCCAGG ACCACCTGGG ATCCCAGGTA TGAAGGGGGA GCCAGGCAGT
    ATAATCATGT CATCTCTGCC AGGGCCAAAG GGTAATCAAG GATACCCAGG TCCTCCTGGA
    ATACAAGGTC CACCTGGTCC CAAGGGTGTA TCAGGGCCAA GTGGTCCTCC AGGACCACCA
    GGTTTAATGG GCCCTCCTGG CCCACCAGGC CCACCGGGAC CAAAGGGGAA TATGGGCTTA
    AATTTCCAGG GACCCAAAGG CGAAAAAGGT GAACAAGGTC TTCAGGGTCC ACCTGGTCCA
    CCTGGGCAGA TCAGTGAACA GAAAAGACCA ATTGACCTAG ATTTTCAGAA AGGCGATCAG
    GGAGTTCCCG GTGATAGAGG GCCTCCTGGA CCTCCGGGAA TACCTGGTCC TCCCGGTCCT
    CCTGGTGGTG TGAAAGGTGA AAAGGGTGAG CCCGGAGAGC CAGGCAAAAG AGGTAAACCA
    GGCAAAGATG GAGAGACTGG TCAACCAGGA ATCCCAGGTT TGCCTGGTGA TCCTGGTTAC
    CCTGGTGAAC CAGGAAGGCA TGGTGAAAAG GGCCAAAAGG GTGATACTGG CCCACCTGGA
    CCACCTGGAT TTGTAATTCC TACACCTGGA ACTGGTGTGA CTATTGGAGC AAAAGGAAAT
    ATTGGGCAAC CTGGTTTGCC TGGAGAAAAA GGAGAGCGAG GATTTCCTGG CATACAAGGT
    CCACCTGGCT TTCCTGGACC TCCAGGGACA GCTGTCATGG GTCCCCCTGG TCCTCCTGGA
    TATCCTGGTG AAAGGGGCCA GAAAGGTGAT GAAGGTCCCC CTGGAATTTG TATTCCTGGA
    TCTCCTGGAC TTGATGGACA GCCTGGAGCT CCTGGCTTTC CAGGACCTCC TGGTCCTCCT
    GGCCCCCAGT TACCCCCGAG TGATGAAATC TGTAAAGCAG GCCCTCCAGG GCCCCCAGGA
    TCTCCAGGTG ACCAAGGACT CCAAGGAGAG CAAGGAGTAA AAGGTGACAA AGGTGATACT
    TGCTTCAACT GTATTGGAAC TGGCATTTCA GGGCCTCCAG GCCAACCAGG TTTACCAGGT
    CTCCCAGGTC CTCCAGGATC TCTTGGAATC CCTGGAGAGA AGGGCGAGAA AGGACAAGCT
    GGGACAACTG GTCCAAAAGG ATTGCCAGGC ATTCCTGGAC CTCCAGGTGC TCCAGGCTTT
    CCAGGGTCTA AGGGTGACCC TGGTGATGTT CTCACTCTTC CGGGAATGAA AGGTGAAAAA
    GGAGAATTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTC TACCTGGTAG TCCTGGAAAG
    GATGGTTTGC CAGGACTTCC TGGCCCCAAA GGAGAGCCCG GTGGAATCAC TTTTAAGGGT
    GAAAGGGGTC CTCCTGGGAA CCCAGGTTTA CCAGGTCTCC CAGGAAATAT GGGACCTACA
    GGCCCTGTTG GTTTTGGGCC TCCAGGCCCC GTAGGTGAAA AAGGCATACA AGGTGTGGCG
    GGAAATCCAG GCCAGCCAGG ATTACCAGGT CCTAAAGGTG ATCCAGGCCA GACCATCAGC
    CAGCCAGGAA AGCCTGGCTT GCCAGGGAAT CCAGGCAGAG ATGGTGAAGT GGGTCTTCCA
    GGTGATCCTG GACTTCCAGG CCAACCAGGC CTACCAGGAA TACCTGGTAG CAAAGGAGAA
    CCAGGTATCC CTGGGATTGG GCCTCCAGGA CCACCTGGTC CCAAAGGTTT TCCAGGAATT
    CCTGGACCTC CAGGACCTCC TGGGGCCCCT GGAAGAATGG GCCCAGAAGG GCCTCCTGGG
    TTACCGGGCT TTCCAGGACC CAAGGGAGAG CCAGGATTTG GGTTACCTGG GCCACCTGGG
    CCACCAGGAT TCCCAGGTTT CAAAGGAACA CCTGGTCCAA AAGGTGATCG TGGTTTACCA
    GGACCTCCAG GTCCTCCAGG ACACACTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAT
    GCTGGACCAA ATGGACAACC TGGCCCAATA GGGCCACCAG GGCTGCCAGG AATTGGTCTT
    CAGGGACCAC CAGGACCACC AGGGACTCCA GGGCCAATAG GCCAACCTGG CTTACATGGA
    ATACCAGGAG AGAAGGGAGA TCCAGGACCT CCTGGGCTTG ATGTTCCAGG ACTCCCTGGA
    GAGAGAGGTA GTCCAGGGCT CCCTGGAGCC CCTGGTCTTA TAGGACCTCC AGGATCACCT
    GGTATCCCTG GAAGAGCAGG TGTCCCTGGA TTTCCAGGTG TCAAAGGCGA AATGGGTATG
    ATGGGACCTC CAGGCCCACC GGGACCTTTG GGTACTCCTG GCAGGAGTGG TGCTCCTGGT
    CTTAAAGGTG ATGACGGCAT GCAAGGTCAG CCAGGTCTCC CTGGCCCTGT AGGAGAAAAG
    GGTGGTAAGG GAGAACCTGG CCTTCCAGGT CCTCCTGGAC CAATGCGTCC AGATTTCCTG
    GGATCAAAAG GAGAAAAGGG TGATCCTGGC TTACCAGGTA TTCCTGGACT TTCCGGACCA
    AAAGGCTACC AGGGATTACC TGGAGACCCA GGGCAACCTG GGCTGAGTGG ACAGCCGGGA
    TCACCAGGAC TACCAGGTCC CAAAGGTACT CCTGGTCTCC CTGGTCCACC AGGACTTACC
    GGACCTCCTG GACCTAAAGG AAACATTGGC GACATGGGTT TTCCTGGCCC TCAAGGTGTA
    GATGGGCCTC CTGGACCTCC TGGAGGTCCT GGACAACCTG GCTCTCCCGG GTTGCCTGGC
    CAGAAAGGAA GTAAAGGAGA ACCTGGAGTT TCAGGCATTG GTCTCCCTGG TCTTCCTGGT
    CCAAAGGGTG AACCTGGTCT GCCTGGATAT CCAGGAAACC CAGGTATCAA AGGTTCTATG
    GGCGAGGCTG GTCTGCCTGG CTTACCAGGA AGCCCTGGAG CAAAAGGTCA ACCCGGCCTG
    CCCGGATTCC CAGGAACACC AGGGCTTCCT GGACCAAAGG GTATGAATGG TCCTCCTGGT
    AACCCTGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGGCGTCC TGGACCACCA
    GGACCTCCTG GTGAAAAAGG CAAGCCAGGT CAAGATGGCA TTCCTGGACC AGCAGGACAA
    AAGGGAGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CTCCTGGACT CCCAGGACTT
    TCTGGTCAAA AGGGTGATGG AGGAGCCCCT GGCCTTCCAG GAAATCCTGG CCTTCCAGGT
    TCAAAGGGTG AACCAGGCTT TCAGGGATTC CCTGGTATGC CAGGTCCCCC AGGTCCCCCT
    GGTTCTCCTG GCCCATCTTT GGAAGGTCCC AAAGGAAACC CTGGGCCTCA AGGCCCTCCT
    GGGAGACCAG GTCCACCTGG TACAGAAGGT CCTCGTGGCC CCCCTGGAAA TGGAGGTATT
    AAAGGAGAGA GGGGAAATCC AGGTCCACCT GGGCAACCAG GCTTACCTGG CTTGAAAGGA
    GATCAAGGAC CACCAGGCCT TCCGGGTAAC CCTGGCCGAC CAGGTCTTAA TGGAATGAAA
    GGAGATCCTG GTCTCCCTGG TGTTCCAGGA TTCCCAGGAA TGAAAGGACC CATTGGAGTA
    CCAGGCTCAA CTGGCCCTGA TGGGGAACCA GGTCTTACGG GGCCCCCAGG TCCTCCTGGG
    TTGCCTGGTC CTTCAGGACA GAGTATTGTA ATCAAAGGAG ATCCTGGACC TCCAGGAATT
    CCTGGACAAC CTGGATTAAA AGGTCTACCA GGACTTCCAG GCCCTCAAGG TCTACCAGGT
    CCAATTGGCC CTCCAGGAGA TCCAGGACGC AATGGACTCC CAGGCTTTGA TGGTGCAGGA
    GGGCGCAAAG GAGACCCAGG CCTGCCGGGG CAGCCAGGTA GCCGTGGATT GGATGGTCCC
    CCAGGACCAG ATGGATTGCA AGGACCTCCA GGGCCCCCGG GAACCACCTC TGTTGCCCAT
    GGATTCCTCA TCACACGTCA CAGTCAGACA ACAGATGCAC CACAATGCCC ACAGGGAACA
    GTCCATATCT ATGATGGCTT TTCTCTCTTA TATGTACAAG GAAATAAAAG AGCCCATGGT
    CAAGACTTGG GGACAGCTGG CAGCTGCCTT CGTCGCTTCA GTACCATGCC TTTCATGTTC
    TGCAACATTA ACAACGTTTG TAACTTTGCT TCAAGAAATG ACTATTCTTA CTGGCTTTCC
    ACCCCAGAGC CCATGCCAAT GAACATGGAA CCCCTGAAGG GCCAGAGCAT CCAGCCATTC
    ATTAGTCGAT GTGCAGTATG TGAGGCACCA GCTGTGGTGA TTGCAGTTCA CAGCCAGACT
    ATTCAGATTC CTCACTGTCC TCAGGGGTGG GACTCTCTGT GGATTGGCTA TTCATTCATG
    ATGCATACAA GTGCAGGAGC AGAAGGCTCT GGCCAAGCCC TAGCCTCTCC TGGCTCCTGT
    TTGGAAGAAT TTCGTTCAGC TCCCTTCATC GAATGTCATG GACGAGGGAC ATGCAATTAC
    TATGCCAATT CTTACAGCTT TTGGCTGGCC ACTGTTGATA TGTCAGACAT GTTCAGTAAA
    CCTCAGTCAG AGACACTGAA AGCAGGCGAC TTGAGGACAC GTATTAGCCG ATGTCAAGTA
    TGCATGAAAA GGACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa48575
    Clone ID Related Accession (Same CDS sequence) XM_008758534.1 , XM_008773475.1
    Accession Version XM_008758534.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5067bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product collagen alpha-5(IV) chain isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000089.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCAAGTGC CTGGAGTGTG CCTGGCCGCC GGCTGGTTCT TACTGGCCCT GTGTCTGTGG 
    GGGCAGCCGG GAGAGGCTGC GGCCTGCCAT GGGTGTTCTT CTGGATCAAA ATGTGACTGC
    AGTGGTATAA AAGGAGAAAA GGGAGAACGG GGCTTTCCAG GCTTAGAAGG CCATCCAGGT
    TTACCAGGAT TTCCAGGTCC AGAAGGGCCT CCAGGACCTC GGGGACAAAA GGGTGATGAT
    GGAATTCCAG GGCCACCAGG ACCAAAAGGA ATCAGAGGTC CTCCTGGACT TCCTGGATTT
    CCAGGGACAC CAGGCCTTCC TGGGATGCCA GGCCACGATG GGGCTCCAGG ACCTCAAGGT
    ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGCGGATTTC CAGGCAGTCC AGGCTTTCCC
    GGTTTACAAG GACCTCCAGG ACCACCTGGG ATCCCAGGTA TGAAGGGGGA GCCAGGCAGT
    ATAATCATGT CATCTCTGCC AGGGCCAAAG GGTAATCAAG GATACCCAGG TCCTCCTGGA
    ATACAAGGTC CACCTGGTCC CAAGGGTGTA TCAGGGCCAA GTGGTCCTCC AGGACCACCA
    GGTTTAATGG GCCCTCCTGG CCCACCAGGC CCACCGGGAC CAAAGGGGAA TATGGGCTTA
    AATTTCCAGG GACCCAAAGG CGAAAAAGGT GAACAAGGTC TTCAGGGTCC ACCTGGTCCA
    CCTGGGCAGA TCAGTGAACA GAAAAGACCA ATTGACCTAG ATTTTCAGAA AGGCGATCAG
    GGAGTTCCCG GTGATAGAGG GCCTCCTGGA CCTCCGGGAA TACCTGGTCC TCCCGGTCCT
    CCTGGTGGTG TGAAAGGTGA AAAGGGTGAG CCCGGAGAGC CAGGCAAAAG AGGTAAACCA
    GGCAAAGATG GAGAGACTGG TCAACCAGGA ATCCCAGGTT TGCCTGGTGA TCCTGGTTAC
    CCTGGTGAAC CAGGAAGGCA TGGTGAAAAG GGCCAAAAGG GTGATACTGG CCCACCTGGA
    CCACCTGGAT TTGTAATTCC TACACCTGGA ACTGGTGTGA CTATTGGAGC AAAAGGAAAT
    ATTGGGCAAC CTGGTTTGCC TGGAGAAAAA GGAGAGCGAG GATTTCCTGG CATACAAGGT
    CCACCTGGCT TTCCTGGACC TCCAGGGACA GCTGTCATGG GTCCCCCTGG TCCTCCTGGA
    TATCCTGGTG AAAGGGGCCA GAAAGGTGAT GAAGGTCCCC CTGGAATTTG TATTCCTGGA
    TCTCCTGGAC TTGATGGACA GCCTGGAGCT CCTGGCTTTC CAGGACCTCC TGGTCCTCCT
    GGCCCCCAGT TACCCCCGAG TGATGAAATC TGTAAAGCAG GCCCTCCAGG GCCCCCAGGA
    TCTCCAGGTG ACCAAGGACT CCAAGGAGAG CAAGGAGTAA AAGGTGACAA AGGTGATACT
    TGCTTCAACT GTATTGGAAC TGGCATTTCA GGGCCTCCAG GCCAACCAGG TTTACCAGGT
    CTCCCAGGTC CTCCAGGATC TCTTGGAATC CCTGGAGAGA AGGGCGAGAA AGGACAAGCT
    GGGACAACTG GTCCAAAAGG ATTGCCAGGC ATTCCTGGAC CTCCAGGTGC TCCAGGCTTT
    CCAGGGTCTA AGGGTGACCC TGGTGATGTT CTCACTCTTC CGGGAATGAA AGGTGAAAAA
    GGAGAATTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTC TACCTGGTAG TCCTGGAAAG
    GATGGTTTGC CAGGACTTCC TGGCCCCAAA GGAGAGCCCG GTGGAATCAC TTTTAAGGGT
    GAAAGGGGTC CTCCTGGGAA CCCAGGTTTA CCAGGTCTCC CAGGAAATAT GGGACCTACA
    GGCCCTGTTG GTTTTGGGCC TCCAGGCCCC GTAGGTGAAA AAGGCATACA AGGTGTGGCG
    GGAAATCCAG GCCAGCCAGG ATTACCAGGT CCTAAAGGTG ATCCAGGCCA GACCATCAGC
    CAGCCAGGAA AGCCTGGCTT GCCAGGGAAT CCAGGCAGAG ATGGTGAAGT GGGTCTTCCA
    GGTGATCCTG GACTTCCAGG CCAACCAGGC CTACCAGGAA TACCTGGTAG CAAAGGAGAA
    CCAGGTATCC CTGGGATTGG GCCTCCAGGA CCACCTGGTC CCAAAGGTTT TCCAGGAATT
    CCTGGACCTC CAGGACCTCC TGGGGCCCCT GGAAGAATGG GCCCAGAAGG GCCTCCTGGG
    TTACCGGGCT TTCCAGGACC CAAGGGAGAG CCAGGATTTG GGTTACCTGG GCCACCTGGG
    CCACCAGGAT TCCCAGGTTT CAAAGGAACA CCTGGTCCAA AAGGTGATCG TGGTTTACCA
    GGACCTCCAG GTCCTCCAGG ACACACTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAT
    GCTGGACCAA ATGGACAACC TGGCCCAATA GGGCCACCAG GGCTGCCAGG AATTGGTCTT
    CAGGGACCAC CAGGACCACC AGGGACTCCA GGGCCAATAG GCCAACCTGG CTTACATGGA
    ATACCAGGAG AGAAGGGAGA TCCAGGACCT CCTGGGCTTG ATGTTCCAGG ACTCCCTGGA
    GAGAGAGGTA GTCCAGGGCT CCCTGGAGCC CCTGGTCTTA TAGGACCTCC AGGATCACCT
    GGTATCCCTG GAAGAGCAGG TGTCCCTGGA TTTCCAGGTG TCAAAGGCGA AATGGGTATG
    ATGGGACCTC CAGGCCCACC GGGACCTTTG GGTACTCCTG GCAGGAGTGG TGCTCCTGGT
    CTTAAAGGTG ATGACGGCAT GCAAGGTCAG CCAGGTCTCC CTGGCCCTGT AGGAGAAAAG
    GGTGGTAAGG GAGAACCTGG CCTTCCAGGT CCTCCTGGAC CAATGCGTCC AGATTTCCTG
    GGATCAAAAG GAGAAAAGGG TGATCCTGGC TTACCAGGTA TTCCTGGACT TTCCGGACCA
    AAAGGCTACC AGGGATTACC TGGAGACCCA GGGCAACCTG GGCTGAGTGG ACAGCCGGGA
    TCACCAGGAC TACCAGGTCC CAAAGGTACT CCTGGTCTCC CTGGTCCACC AGGACTTACC
    GGACCTCCTG GACCTAAAGG AAACATTGGC GACATGGGTT TTCCTGGCCC TCAAGGTGTA
    GATGGGCCTC CTGGACCTCC TGGAGGTCCT GGACAACCTG GCTCTCCCGG GTTGCCTGGC
    CAGAAAGGAA GTAAAGGAGA ACCTGGAGTT TCAGGCATTG GTCTCCCTGG TCTTCCTGGT
    CCAAAGGGTG AACCTGGTCT GCCTGGATAT CCAGGAAACC CAGGTATCAA AGGTTCTATG
    GGCGAGGCTG GTCTGCCTGG CTTACCAGGA AGCCCTGGAG CAAAAGGTCA ACCCGGCCTG
    CCCGGATTCC CAGGAACACC AGGGCTTCCT GGACCAAAGG GTATGAATGG TCCTCCTGGT
    AACCCTGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGGCGTCC TGGACCACCA
    GGACCTCCTG GTGAAAAAGG CAAGCCAGGT CAAGATGGCA TTCCTGGACC AGCAGGACAA
    AAGGGAGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CTCCTGGACT CCCAGGACTT
    TCTGGTCAAA AGGGTGATGG AGGAGCCCCT GGCCTTCCAG GAAATCCTGG CCTTCCAGGT
    TCAAAGGGTG AACCAGGCTT TCAGGGATTC CCTGGTATGC CAGGTCCCCC AGGTCCCCCT
    GGTTCTCCTG GCCCATCTTT GGAAGGTCCC AAAGGAAACC CTGGGCCTCA AGGCCCTCCT
    GGGAGACCAG GTTTTCAAGG TCCACCTGGT ACAGAAGGTC CTCGTGGCCC CCCTGGAAAT
    GGAGGTATTA AAGGAGAGAG GGGAAATCCA GGTCCACCTG GGCAACCAGG CTTACCTGGC
    TTGAAAGGAG ATCAAGGACC ACCAGGCCTT CCGGGTAACC CTGGCCGACC AGGTCTTAAT
    GGAATGAAAG GAGATCCTGG TCTCCCTGGT GTTCCAGGAT TCCCAGGAAT GAAAGGACCC
    ATTGGAGTAC CAGGCTCAAC TGGCCCTGAT GGGGAACCAG GTCTTACGGG GCCCCCAGGT
    CCTCCTGGGT TGCCTGGTCC TTCAGGACAG AGTATTGTAA TCAAAGGAGA TCCTGGACCT
    CCAGGAATTC CTGGACAACC TGGATTAAAA GGTCTACCAG GACTTCCAGG CCCTCAAGGT
    CTACCAGGTC CAATTGGCCC TCCAGGAGAT CCAGGACGCA ATGGACTCCC AGGCTTTGAT
    GGTGCAGGAG GGCGCAAAGG AGACCCAGGC CTGCCGGGGC AGCCAGGTAG CCGTGGATTG
    GATGGTCCCC CAGGACCAGA TGGATTGCAA GGACCTCCAG GGCCCCCGGG AACCACCTCT
    GTTGCCCATG GATTCCTCAT CACACGTCAC AGTCAGACAA CAGATGCACC ACAATGCCCA
    CAGGGAACAG TCCATATCTA TGATGGCTTT TCTCTCTTAT ATGTACAAGG AAATAAAAGA
    GCCCATGGTC AAGACTTGGG GACAGCTGGC AGCTGCCTTC GTCGCTTCAG TACCATGCCT
    TTCATGTTCT GCAACATTAA CAACGTTTGT AACTTTGCTT CAAGAAATGA CTATTCTTAC
    TGGCTTTCCA CCCCAGAGCC CATGCCAATG AACATGGAAC CCCTGAAGGG CCAGAGCATC
    CAGCCATTCA TTAGTCGATG TGCAGTATGT GAGGCACCAG CTGTGGTGAT TGCAGTTCAC
    AGCCAGACTA TTCAGATTCC TCACTGTCCT CAGGGGTGGG ACTCTCTGTG GATTGGCTAT
    TCATTCATGA TGCATACAAG TGCAGGAGCA GAAGGCTCTG GCCAAGCCCT AGCCTCTCCT
    GGCTCCTGTT TGGAAGAATT TCGTTCAGCT CCCTTCATCG AATGTCATGG ACGAGGGACA
    TGCAATTACT ATGCCAATTC TTACAGCTTT TGGCTGGCCA CTGTTGATAT GTCAGACATG
    TTCAGTAAAC CTCAGTCAGA GACACTGAAA GCAGGCGACT TGAGGACACG TATTAGCCGA
    TGTCAAGTAT GCATGAAAAG GACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_008756756.1
    CDS400..5466
    Translation

    Target ORF information:

    RefSeq Version XM_008758534.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus collagen type IV alpha 5 chain (Col4a5), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_008758534.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCAAGTGC CTGGAGTGTG CCTGGCCGCC GGCTGGTTCT TACTGGCCCT GTGTCTGTGG 
    GGGCAGCCGG GAGAGGCTGC GGCCTGCCAT GGGTGTTCTT CTGGATCAAA ATGTGACTGC
    AGTGGTATAA AAGGAGAAAA GGGAGAACGG GGCTTTCCAG GCTTAGAAGG CCATCCAGGT
    TTACCAGGAT TTCCAGGTCC AGAAGGGCCT CCAGGACCTC GGGGACAAAA GGGTGATGAT
    GGAATTCCAG GGCCACCAGG ACCAAAAGGA ATCAGAGGTC CTCCTGGACT TCCTGGATTT
    CCAGGGACAC CAGGCCTTCC TGGGATGCCA GGCCACGATG GGGCTCCAGG ACCTCAAGGT
    ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGCGGATTTC CAGGCAGTCC AGGCTTTCCC
    GGTTTACAAG GACCTCCAGG ACCACCTGGG ATCCCAGGTA TGAAGGGGGA GCCAGGCAGT
    ATAATCATGT CATCTCTGCC AGGGCCAAAG GGTAATCAAG GATACCCAGG TCCTCCTGGA
    ATACAAGGTC CACCTGGTCC CAAGGGTGTA TCAGGGCCAA GTGGTCCTCC AGGACCACCA
    GGTTTAATGG GCCCTCCTGG CCCACCAGGC CCACCGGGAC CAAAGGGGAA TATGGGCTTA
    AATTTCCAGG GACCCAAAGG CGAAAAAGGT GAACAAGGTC TTCAGGGTCC ACCTGGTCCA
    CCTGGGCAGA TCAGTGAACA GAAAAGACCA ATTGACCTAG ATTTTCAGAA AGGCGATCAG
    GGAGTTCCCG GTGATAGAGG GCCTCCTGGA CCTCCGGGAA TACCTGGTCC TCCCGGTCCT
    CCTGGTGGTG TGAAAGGTGA AAAGGGTGAG CCCGGAGAGC CAGGCAAAAG AGGTAAACCA
    GGCAAAGATG GAGAGACTGG TCAACCAGGA ATCCCAGGTT TGCCTGGTGA TCCTGGTTAC
    CCTGGTGAAC CAGGAAGGCA TGGTGAAAAG GGCCAAAAGG GTGATACTGG CCCACCTGGA
    CCACCTGGAT TTGTAATTCC TACACCTGGA ACTGGTGTGA CTATTGGAGC AAAAGGAAAT
    ATTGGGCAAC CTGGTTTGCC TGGAGAAAAA GGAGAGCGAG GATTTCCTGG CATACAAGGT
    CCACCTGGCT TTCCTGGACC TCCAGGGACA GCTGTCATGG GTCCCCCTGG TCCTCCTGGA
    TATCCTGGTG AAAGGGGCCA GAAAGGTGAT GAAGGTCCCC CTGGAATTTG TATTCCTGGA
    TCTCCTGGAC TTGATGGACA GCCTGGAGCT CCTGGCTTTC CAGGACCTCC TGGTCCTCCT
    GGCCCCCAGT TACCCCCGAG TGATGAAATC TGTAAAGCAG GCCCTCCAGG GCCCCCAGGA
    TCTCCAGGTG ACCAAGGACT CCAAGGAGAG CAAGGAGTAA AAGGTGACAA AGGTGATACT
    TGCTTCAACT GTATTGGAAC TGGCATTTCA GGGCCTCCAG GCCAACCAGG TTTACCAGGT
    CTCCCAGGTC CTCCAGGATC TCTTGGAATC CCTGGAGAGA AGGGCGAGAA AGGACAAGCT
    GGGACAACTG GTCCAAAAGG ATTGCCAGGC ATTCCTGGAC CTCCAGGTGC TCCAGGCTTT
    CCAGGGTCTA AGGGTGACCC TGGTGATGTT CTCACTCTTC CGGGAATGAA AGGTGAAAAA
    GGAGAATTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTC TACCTGGTAG TCCTGGAAAG
    GATGGTTTGC CAGGACTTCC TGGCCCCAAA GGAGAGCCCG GTGGAATCAC TTTTAAGGGT
    GAAAGGGGTC CTCCTGGGAA CCCAGGTTTA CCAGGTCTCC CAGGAAATAT GGGACCTACA
    GGCCCTGTTG GTTTTGGGCC TCCAGGCCCC GTAGGTGAAA AAGGCATACA AGGTGTGGCG
    GGAAATCCAG GCCAGCCAGG ATTACCAGGT CCTAAAGGTG ATCCAGGCCA GACCATCAGC
    CAGCCAGGAA AGCCTGGCTT GCCAGGGAAT CCAGGCAGAG ATGGTGAAGT GGGTCTTCCA
    GGTGATCCTG GACTTCCAGG CCAACCAGGC CTACCAGGAA TACCTGGTAG CAAAGGAGAA
    CCAGGTATCC CTGGGATTGG GCCTCCAGGA CCACCTGGTC CCAAAGGTTT TCCAGGAATT
    CCTGGACCTC CAGGACCTCC TGGGGCCCCT GGAAGAATGG GCCCAGAAGG GCCTCCTGGG
    TTACCGGGCT TTCCAGGACC CAAGGGAGAG CCAGGATTTG GGTTACCTGG GCCACCTGGG
    CCACCAGGAT TCCCAGGTTT CAAAGGAACA CCTGGTCCAA AAGGTGATCG TGGTTTACCA
    GGACCTCCAG GTCCTCCAGG ACACACTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAT
    GCTGGACCAA ATGGACAACC TGGCCCAATA GGGCCACCAG GGCTGCCAGG AATTGGTCTT
    CAGGGACCAC CAGGACCACC AGGGACTCCA GGGCCAATAG GCCAACCTGG CTTACATGGA
    ATACCAGGAG AGAAGGGAGA TCCAGGACCT CCTGGGCTTG ATGTTCCAGG ACTCCCTGGA
    GAGAGAGGTA GTCCAGGGCT CCCTGGAGCC CCTGGTCTTA TAGGACCTCC AGGATCACCT
    GGTATCCCTG GAAGAGCAGG TGTCCCTGGA TTTCCAGGTG TCAAAGGCGA AATGGGTATG
    ATGGGACCTC CAGGCCCACC GGGACCTTTG GGTACTCCTG GCAGGAGTGG TGCTCCTGGT
    CTTAAAGGTG ATGACGGCAT GCAAGGTCAG CCAGGTCTCC CTGGCCCTGT AGGAGAAAAG
    GGTGGTAAGG GAGAACCTGG CCTTCCAGGT CCTCCTGGAC CAATGCGTCC AGATTTCCTG
    GGATCAAAAG GAGAAAAGGG TGATCCTGGC TTACCAGGTA TTCCTGGACT TTCCGGACCA
    AAAGGCTACC AGGGATTACC TGGAGACCCA GGGCAACCTG GGCTGAGTGG ACAGCCGGGA
    TCACCAGGAC TACCAGGTCC CAAAGGTACT CCTGGTCTCC CTGGTCCACC AGGACTTACC
    GGACCTCCTG GACCTAAAGG AAACATTGGC GACATGGGTT TTCCTGGCCC TCAAGGTGTA
    GATGGGCCTC CTGGACCTCC TGGAGGTCCT GGACAACCTG GCTCTCCCGG GTTGCCTGGC
    CAGAAAGGAA GTAAAGGAGA ACCTGGAGTT TCAGGCATTG GTCTCCCTGG TCTTCCTGGT
    CCAAAGGGTG AACCTGGTCT GCCTGGATAT CCAGGAAACC CAGGTATCAA AGGTTCTATG
    GGCGAGGCTG GTCTGCCTGG CTTACCAGGA AGCCCTGGAG CAAAAGGTCA ACCCGGCCTG
    CCCGGATTCC CAGGAACACC AGGGCTTCCT GGACCAAAGG GTATGAATGG TCCTCCTGGT
    AACCCTGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGGCGTCC TGGACCACCA
    GGACCTCCTG GTGAAAAAGG CAAGCCAGGT CAAGATGGCA TTCCTGGACC AGCAGGACAA
    AAGGGAGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CTCCTGGACT CCCAGGACTT
    TCTGGTCAAA AGGGTGATGG AGGAGCCCCT GGCCTTCCAG GAAATCCTGG CCTTCCAGGT
    TCAAAGGGTG AACCAGGCTT TCAGGGATTC CCTGGTATGC CAGGTCCCCC AGGTCCCCCT
    GGTTCTCCTG GCCCATCTTT GGAAGGTCCC AAAGGAAACC CTGGGCCTCA AGGCCCTCCT
    GGGAGACCAG GTTTTCAAGG TCCACCTGGT ACAGAAGGTC CTCGTGGCCC CCCTGGAAAT
    GGAGGTATTA AAGGAGAGAG GGGAAATCCA GGTCCACCTG GGCAACCAGG CTTACCTGGC
    TTGAAAGGAG ATCAAGGACC ACCAGGCCTT CCGGGTAACC CTGGCCGACC AGGTCTTAAT
    GGAATGAAAG GAGATCCTGG TCTCCCTGGT GTTCCAGGAT TCCCAGGAAT GAAAGGACCC
    ATTGGAGTAC CAGGCTCAAC TGGCCCTGAT GGGGAACCAG GTCTTACGGG GCCCCCAGGT
    CCTCCTGGGT TGCCTGGTCC TTCAGGACAG AGTATTGTAA TCAAAGGAGA TCCTGGACCT
    CCAGGAATTC CTGGACAACC TGGATTAAAA GGTCTACCAG GACTTCCAGG CCCTCAAGGT
    CTACCAGGTC CAATTGGCCC TCCAGGAGAT CCAGGACGCA ATGGACTCCC AGGCTTTGAT
    GGTGCAGGAG GGCGCAAAGG AGACCCAGGC CTGCCGGGGC AGCCAGGTAG CCGTGGATTG
    GATGGTCCCC CAGGACCAGA TGGATTGCAA GGACCTCCAG GGCCCCCGGG AACCACCTCT
    GTTGCCCATG GATTCCTCAT CACACGTCAC AGTCAGACAA CAGATGCACC ACAATGCCCA
    CAGGGAACAG TCCATATCTA TGATGGCTTT TCTCTCTTAT ATGTACAAGG AAATAAAAGA
    GCCCATGGTC AAGACTTGGG GACAGCTGGC AGCTGCCTTC GTCGCTTCAG TACCATGCCT
    TTCATGTTCT GCAACATTAA CAACGTTTGT AACTTTGCTT CAAGAAATGA CTATTCTTAC
    TGGCTTTCCA CCCCAGAGCC CATGCCAATG AACATGGAAC CCCTGAAGGG CCAGAGCATC
    CAGCCATTCA TTAGTCGATG TGCAGTATGT GAGGCACCAG CTGTGGTGAT TGCAGTTCAC
    AGCCAGACTA TTCAGATTCC TCACTGTCCT CAGGGGTGGG ACTCTCTGTG GATTGGCTAT
    TCATTCATGA TGCATACAAG TGCAGGAGCA GAAGGCTCTG GCCAAGCCCT AGCCTCTCCT
    GGCTCCTGTT TGGAAGAATT TCGTTCAGCT CCCTTCATCG AATGTCATGG ACGAGGGACA
    TGCAATTACT ATGCCAATTC TTACAGCTTT TGGCTGGCCA CTGTTGATAT GTCAGACATG
    TTCAGTAAAC CTCAGTCAGA GACACTGAAA GCAGGCGACT TGAGGACACG TATTAGCCGA
    TGTCAAGTAT GCATGAAAAG GACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa48575
    Clone ID Related Accession (Same CDS sequence) XM_008758534.1 , XM_008773475.1
    Accession Version XM_008773475.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5067bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product collagen alpha-5(IV) chain isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCAAGTGC CTGGAGTGTG CCTGGCCGCC GGCTGGTTCT TACTGGCCCT GTGTCTGTGG 
    GGGCAGCCGG GAGAGGCTGC GGCCTGCCAT GGGTGTTCTT CTGGATCAAA ATGTGACTGC
    AGTGGTATAA AAGGAGAAAA GGGAGAACGG GGCTTTCCAG GCTTAGAAGG CCATCCAGGT
    TTACCAGGAT TTCCAGGTCC AGAAGGGCCT CCAGGACCTC GGGGACAAAA GGGTGATGAT
    GGAATTCCAG GGCCACCAGG ACCAAAAGGA ATCAGAGGTC CTCCTGGACT TCCTGGATTT
    CCAGGGACAC CAGGCCTTCC TGGGATGCCA GGCCACGATG GGGCTCCAGG ACCTCAAGGT
    ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGCGGATTTC CAGGCAGTCC AGGCTTTCCC
    GGTTTACAAG GACCTCCAGG ACCACCTGGG ATCCCAGGTA TGAAGGGGGA GCCAGGCAGT
    ATAATCATGT CATCTCTGCC AGGGCCAAAG GGTAATCAAG GATACCCAGG TCCTCCTGGA
    ATACAAGGTC CACCTGGTCC CAAGGGTGTA TCAGGGCCAA GTGGTCCTCC AGGACCACCA
    GGTTTAATGG GCCCTCCTGG CCCACCAGGC CCACCGGGAC CAAAGGGGAA TATGGGCTTA
    AATTTCCAGG GACCCAAAGG CGAAAAAGGT GAACAAGGTC TTCAGGGTCC ACCTGGTCCA
    CCTGGGCAGA TCAGTGAACA GAAAAGACCA ATTGACCTAG ATTTTCAGAA AGGCGATCAG
    GGAGTTCCCG GTGATAGAGG GCCTCCTGGA CCTCCGGGAA TACCTGGTCC TCCCGGTCCT
    CCTGGTGGTG TGAAAGGTGA AAAGGGTGAG CCCGGAGAGC CAGGCAAAAG AGGTAAACCA
    GGCAAAGATG GAGAGACTGG TCAACCAGGA ATCCCAGGTT TGCCTGGTGA TCCTGGTTAC
    CCTGGTGAAC CAGGAAGGCA TGGTGAAAAG GGCCAAAAGG GTGATACTGG CCCACCTGGA
    CCACCTGGAT TTGTAATTCC TACACCTGGA ACTGGTGTGA CTATTGGAGC AAAAGGAAAT
    ATTGGGCAAC CTGGTTTGCC TGGAGAAAAA GGAGAGCGAG GATTTCCTGG CATACAAGGT
    CCACCTGGCT TTCCTGGACC TCCAGGGACA GCTGTCATGG GTCCCCCTGG TCCTCCTGGA
    TATCCTGGTG AAAGGGGCCA GAAAGGTGAT GAAGGTCCCC CTGGAATTTG TATTCCTGGA
    TCTCCTGGAC TTGATGGACA GCCTGGAGCT CCTGGCTTTC CAGGACCTCC TGGTCCTCCT
    GGCCCCCAGT TACCCCCGAG TGATGAAATC TGTAAAGCAG GCCCTCCAGG GCCCCCAGGA
    TCTCCAGGTG ACCAAGGACT CCAAGGAGAG CAAGGAGTAA AAGGTGACAA AGGTGATACT
    TGCTTCAACT GTATTGGAAC TGGCATTTCA GGGCCTCCAG GCCAACCAGG TTTACCAGGT
    CTCCCAGGTC CTCCAGGATC TCTTGGAATC CCTGGAGAGA AGGGCGAGAA AGGACAAGCT
    GGGACAACTG GTCCAAAAGG ATTGCCAGGC ATTCCTGGAC CTCCAGGTGC TCCAGGCTTT
    CCAGGGTCTA AGGGTGACCC TGGTGATGTT CTCACTCTTC CGGGAATGAA AGGTGAAAAA
    GGAGAATTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTC TACCTGGTAG TCCTGGAAAG
    GATGGTTTGC CAGGACTTCC TGGCCCCAAA GGAGAGCCCG GTGGAATCAC TTTTAAGGGT
    GAAAGGGGTC CTCCTGGGAA CCCAGGTTTA CCAGGTCTCC CAGGAAATAT GGGACCTACA
    GGCCCTGTTG GTTTTGGGCC TCCAGGCCCC GTAGGTGAAA AAGGCATACA AGGTGTGGCG
    GGAAATCCAG GCCAGCCAGG ATTACCAGGT CCTAAAGGTG ATCCAGGCCA GACCATCAGC
    CAGCCAGGAA AGCCTGGCTT GCCAGGGAAT CCAGGCAGAG ATGGTGAAGT GGGTCTTCCA
    GGTGATCCTG GACTTCCAGG CCAACCAGGC CTACCAGGAA TACCTGGTAG CAAAGGAGAA
    CCAGGTATCC CTGGGATTGG GCCTCCAGGA CCACCTGGTC CCAAAGGTTT TCCAGGAATT
    CCTGGACCTC CAGGACCTCC TGGGGCCCCT GGAAGAATGG GCCCAGAAGG GCCTCCTGGG
    TTACCGGGCT TTCCAGGACC CAAGGGAGAG CCAGGATTTG GGTTACCTGG GCCACCTGGG
    CCACCAGGAT TCCCAGGTTT CAAAGGAACA CCTGGTCCAA AAGGTGATCG TGGTTTACCA
    GGACCTCCAG GTCCTCCAGG ACACACTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAT
    GCTGGACCAA ATGGACAACC TGGCCCAATA GGGCCACCAG GGCTGCCAGG AATTGGTCTT
    CAGGGACCAC CAGGACCACC AGGGACTCCA GGGCCAATAG GCCAACCTGG CTTACATGGA
    ATACCAGGAG AGAAGGGAGA TCCAGGACCT CCTGGGCTTG ATGTTCCAGG ACTCCCTGGA
    GAGAGAGGTA GTCCAGGGCT CCCTGGAGCC CCTGGTCTTA TAGGACCTCC AGGATCACCT
    GGTATCCCTG GAAGAGCAGG TGTCCCTGGA TTTCCAGGTG TCAAAGGCGA AATGGGTATG
    ATGGGACCTC CAGGCCCACC GGGACCTTTG GGTACTCCTG GCAGGAGTGG TGCTCCTGGT
    CTTAAAGGTG ATGACGGCAT GCAAGGTCAG CCAGGTCTCC CTGGCCCTGT AGGAGAAAAG
    GGTGGTAAGG GAGAACCTGG CCTTCCAGGT CCTCCTGGAC CAATGCGTCC AGATTTCCTG
    GGATCAAAAG GAGAAAAGGG TGATCCTGGC TTACCAGGTA TTCCTGGACT TTCCGGACCA
    AAAGGCTACC AGGGATTACC TGGAGACCCA GGGCAACCTG GGCTGAGTGG ACAGCCGGGA
    TCACCAGGAC TACCAGGTCC CAAAGGTACT CCTGGTCTCC CTGGTCCACC AGGACTTACC
    GGACCTCCTG GACCTAAAGG AAACATTGGC GACATGGGTT TTCCTGGCCC TCAAGGTGTA
    GATGGGCCTC CTGGACCTCC TGGAGGTCCT GGACAACCTG GCTCTCCCGG GTTGCCTGGC
    CAGAAAGGAA GTAAAGGAGA ACCTGGAGTT TCAGGCATTG GTCTCCCTGG TCTTCCTGGT
    CCAAAGGGTG AACCTGGTCT GCCTGGATAT CCAGGAAACC CAGGTATCAA AGGTTCTATG
    GGCGAGGCTG GTCTGCCTGG CTTACCAGGA AGCCCTGGAG CAAAAGGTCA ACCCGGCCTG
    CCCGGATTCC CAGGAACACC AGGGCTTCCT GGACCAAAGG GTATGAATGG TCCTCCTGGT
    AACCCTGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGGCGTCC TGGACCACCA
    GGACCTCCTG GTGAAAAAGG CAAGCCAGGT CAAGATGGCA TTCCTGGACC AGCAGGACAA
    AAGGGAGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CTCCTGGACT CCCAGGACTT
    TCTGGTCAAA AGGGTGATGG AGGAGCCCCT GGCCTTCCAG GAAATCCTGG CCTTCCAGGT
    TCAAAGGGTG AACCAGGCTT TCAGGGATTC CCTGGTATGC CAGGTCCCCC AGGTCCCCCT
    GGTTCTCCTG GCCCATCTTT GGAAGGTCCC AAAGGAAACC CTGGGCCTCA AGGCCCTCCT
    GGGAGACCAG GTTTTCAAGG TCCACCTGGT ACAGAAGGTC CTCGTGGCCC CCCTGGAAAT
    GGAGGTATTA AAGGAGAGAG GGGAAATCCA GGTCCACCTG GGCAACCAGG CTTACCTGGC
    TTGAAAGGAG ATCAAGGACC ACCAGGCCTT CCGGGTAACC CTGGCCGACC AGGTCTTAAT
    GGAATGAAAG GAGATCCTGG TCTCCCTGGT GTTCCAGGAT TCCCAGGAAT GAAAGGACCC
    ATTGGAGTAC CAGGCTCAAC TGGCCCTGAT GGGGAACCAG GTCTTACGGG GCCCCCAGGT
    CCTCCTGGGT TGCCTGGTCC TTCAGGACAG AGTATTGTAA TCAAAGGAGA TCCTGGACCT
    CCAGGAATTC CTGGACAACC TGGATTAAAA GGTCTACCAG GACTTCCAGG CCCTCAAGGT
    CTACCAGGTC CAATTGGCCC TCCAGGAGAT CCAGGACGCA ATGGACTCCC AGGCTTTGAT
    GGTGCAGGAG GGCGCAAAGG AGACCCAGGC CTGCCGGGGC AGCCAGGTAG CCGTGGATTG
    GATGGTCCCC CAGGACCAGA TGGATTGCAA GGACCTCCAG GGCCCCCGGG AACCACCTCT
    GTTGCCCATG GATTCCTCAT CACACGTCAC AGTCAGACAA CAGATGCACC ACAATGCCCA
    CAGGGAACAG TCCATATCTA TGATGGCTTT TCTCTCTTAT ATGTACAAGG AAATAAAAGA
    GCCCATGGTC AAGACTTGGG GACAGCTGGC AGCTGCCTTC GTCGCTTCAG TACCATGCCT
    TTCATGTTCT GCAACATTAA CAACGTTTGT AACTTTGCTT CAAGAAATGA CTATTCTTAC
    TGGCTTTCCA CCCCAGAGCC CATGCCAATG AACATGGAAC CCCTGAAGGG CCAGAGCATC
    CAGCCATTCA TTAGTCGATG TGCAGTATGT GAGGCACCAG CTGTGGTGAT TGCAGTTCAC
    AGCCAGACTA TTCAGATTCC TCACTGTCCT CAGGGGTGGG ACTCTCTGTG GATTGGCTAT
    TCATTCATGA TGCATACAAG TGCAGGAGCA GAAGGCTCTG GCCAAGCCCT AGCCTCTCCT
    GGCTCCTGTT TGGAAGAATT TCGTTCAGCT CCCTTCATCG AATGTCATGG ACGAGGGACA
    TGCAATTACT ATGCCAATTC TTACAGCTTT TGGCTGGCCA CTGTTGATAT GTCAGACATG
    TTCAGTAAAC CTCAGTCAGA GACACTGAAA GCAGGCGACT TGAGGACACG TATTAGCCGA
    TGTCAAGTAT GCATGAAAAG GACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_008771697.1
    CDS400..5466
    Translation

    Target ORF information:

    RefSeq Version XM_008773475.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus collagen type IV alpha 5 chain (Col4a5), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_008773475.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCAAGTGC CTGGAGTGTG CCTGGCCGCC GGCTGGTTCT TACTGGCCCT GTGTCTGTGG 
    GGGCAGCCGG GAGAGGCTGC GGCCTGCCAT GGGTGTTCTT CTGGATCAAA ATGTGACTGC
    AGTGGTATAA AAGGAGAAAA GGGAGAACGG GGCTTTCCAG GCTTAGAAGG CCATCCAGGT
    TTACCAGGAT TTCCAGGTCC AGAAGGGCCT CCAGGACCTC GGGGACAAAA GGGTGATGAT
    GGAATTCCAG GGCCACCAGG ACCAAAAGGA ATCAGAGGTC CTCCTGGACT TCCTGGATTT
    CCAGGGACAC CAGGCCTTCC TGGGATGCCA GGCCACGATG GGGCTCCAGG ACCTCAAGGT
    ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGCGGATTTC CAGGCAGTCC AGGCTTTCCC
    GGTTTACAAG GACCTCCAGG ACCACCTGGG ATCCCAGGTA TGAAGGGGGA GCCAGGCAGT
    ATAATCATGT CATCTCTGCC AGGGCCAAAG GGTAATCAAG GATACCCAGG TCCTCCTGGA
    ATACAAGGTC CACCTGGTCC CAAGGGTGTA TCAGGGCCAA GTGGTCCTCC AGGACCACCA
    GGTTTAATGG GCCCTCCTGG CCCACCAGGC CCACCGGGAC CAAAGGGGAA TATGGGCTTA
    AATTTCCAGG GACCCAAAGG CGAAAAAGGT GAACAAGGTC TTCAGGGTCC ACCTGGTCCA
    CCTGGGCAGA TCAGTGAACA GAAAAGACCA ATTGACCTAG ATTTTCAGAA AGGCGATCAG
    GGAGTTCCCG GTGATAGAGG GCCTCCTGGA CCTCCGGGAA TACCTGGTCC TCCCGGTCCT
    CCTGGTGGTG TGAAAGGTGA AAAGGGTGAG CCCGGAGAGC CAGGCAAAAG AGGTAAACCA
    GGCAAAGATG GAGAGACTGG TCAACCAGGA ATCCCAGGTT TGCCTGGTGA TCCTGGTTAC
    CCTGGTGAAC CAGGAAGGCA TGGTGAAAAG GGCCAAAAGG GTGATACTGG CCCACCTGGA
    CCACCTGGAT TTGTAATTCC TACACCTGGA ACTGGTGTGA CTATTGGAGC AAAAGGAAAT
    ATTGGGCAAC CTGGTTTGCC TGGAGAAAAA GGAGAGCGAG GATTTCCTGG CATACAAGGT
    CCACCTGGCT TTCCTGGACC TCCAGGGACA GCTGTCATGG GTCCCCCTGG TCCTCCTGGA
    TATCCTGGTG AAAGGGGCCA GAAAGGTGAT GAAGGTCCCC CTGGAATTTG TATTCCTGGA
    TCTCCTGGAC TTGATGGACA GCCTGGAGCT CCTGGCTTTC CAGGACCTCC TGGTCCTCCT
    GGCCCCCAGT TACCCCCGAG TGATGAAATC TGTAAAGCAG GCCCTCCAGG GCCCCCAGGA
    TCTCCAGGTG ACCAAGGACT CCAAGGAGAG CAAGGAGTAA AAGGTGACAA AGGTGATACT
    TGCTTCAACT GTATTGGAAC TGGCATTTCA GGGCCTCCAG GCCAACCAGG TTTACCAGGT
    CTCCCAGGTC CTCCAGGATC TCTTGGAATC CCTGGAGAGA AGGGCGAGAA AGGACAAGCT
    GGGACAACTG GTCCAAAAGG ATTGCCAGGC ATTCCTGGAC CTCCAGGTGC TCCAGGCTTT
    CCAGGGTCTA AGGGTGACCC TGGTGATGTT CTCACTCTTC CGGGAATGAA AGGTGAAAAA
    GGAGAATTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTC TACCTGGTAG TCCTGGAAAG
    GATGGTTTGC CAGGACTTCC TGGCCCCAAA GGAGAGCCCG GTGGAATCAC TTTTAAGGGT
    GAAAGGGGTC CTCCTGGGAA CCCAGGTTTA CCAGGTCTCC CAGGAAATAT GGGACCTACA
    GGCCCTGTTG GTTTTGGGCC TCCAGGCCCC GTAGGTGAAA AAGGCATACA AGGTGTGGCG
    GGAAATCCAG GCCAGCCAGG ATTACCAGGT CCTAAAGGTG ATCCAGGCCA GACCATCAGC
    CAGCCAGGAA AGCCTGGCTT GCCAGGGAAT CCAGGCAGAG ATGGTGAAGT GGGTCTTCCA
    GGTGATCCTG GACTTCCAGG CCAACCAGGC CTACCAGGAA TACCTGGTAG CAAAGGAGAA
    CCAGGTATCC CTGGGATTGG GCCTCCAGGA CCACCTGGTC CCAAAGGTTT TCCAGGAATT
    CCTGGACCTC CAGGACCTCC TGGGGCCCCT GGAAGAATGG GCCCAGAAGG GCCTCCTGGG
    TTACCGGGCT TTCCAGGACC CAAGGGAGAG CCAGGATTTG GGTTACCTGG GCCACCTGGG
    CCACCAGGAT TCCCAGGTTT CAAAGGAACA CCTGGTCCAA AAGGTGATCG TGGTTTACCA
    GGACCTCCAG GTCCTCCAGG ACACACTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAT
    GCTGGACCAA ATGGACAACC TGGCCCAATA GGGCCACCAG GGCTGCCAGG AATTGGTCTT
    CAGGGACCAC CAGGACCACC AGGGACTCCA GGGCCAATAG GCCAACCTGG CTTACATGGA
    ATACCAGGAG AGAAGGGAGA TCCAGGACCT CCTGGGCTTG ATGTTCCAGG ACTCCCTGGA
    GAGAGAGGTA GTCCAGGGCT CCCTGGAGCC CCTGGTCTTA TAGGACCTCC AGGATCACCT
    GGTATCCCTG GAAGAGCAGG TGTCCCTGGA TTTCCAGGTG TCAAAGGCGA AATGGGTATG
    ATGGGACCTC CAGGCCCACC GGGACCTTTG GGTACTCCTG GCAGGAGTGG TGCTCCTGGT
    CTTAAAGGTG ATGACGGCAT GCAAGGTCAG CCAGGTCTCC CTGGCCCTGT AGGAGAAAAG
    GGTGGTAAGG GAGAACCTGG CCTTCCAGGT CCTCCTGGAC CAATGCGTCC AGATTTCCTG
    GGATCAAAAG GAGAAAAGGG TGATCCTGGC TTACCAGGTA TTCCTGGACT TTCCGGACCA
    AAAGGCTACC AGGGATTACC TGGAGACCCA GGGCAACCTG GGCTGAGTGG ACAGCCGGGA
    TCACCAGGAC TACCAGGTCC CAAAGGTACT CCTGGTCTCC CTGGTCCACC AGGACTTACC
    GGACCTCCTG GACCTAAAGG AAACATTGGC GACATGGGTT TTCCTGGCCC TCAAGGTGTA
    GATGGGCCTC CTGGACCTCC TGGAGGTCCT GGACAACCTG GCTCTCCCGG GTTGCCTGGC
    CAGAAAGGAA GTAAAGGAGA ACCTGGAGTT TCAGGCATTG GTCTCCCTGG TCTTCCTGGT
    CCAAAGGGTG AACCTGGTCT GCCTGGATAT CCAGGAAACC CAGGTATCAA AGGTTCTATG
    GGCGAGGCTG GTCTGCCTGG CTTACCAGGA AGCCCTGGAG CAAAAGGTCA ACCCGGCCTG
    CCCGGATTCC CAGGAACACC AGGGCTTCCT GGACCAAAGG GTATGAATGG TCCTCCTGGT
    AACCCTGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGGCGTCC TGGACCACCA
    GGACCTCCTG GTGAAAAAGG CAAGCCAGGT CAAGATGGCA TTCCTGGACC AGCAGGACAA
    AAGGGAGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CTCCTGGACT CCCAGGACTT
    TCTGGTCAAA AGGGTGATGG AGGAGCCCCT GGCCTTCCAG GAAATCCTGG CCTTCCAGGT
    TCAAAGGGTG AACCAGGCTT TCAGGGATTC CCTGGTATGC CAGGTCCCCC AGGTCCCCCT
    GGTTCTCCTG GCCCATCTTT GGAAGGTCCC AAAGGAAACC CTGGGCCTCA AGGCCCTCCT
    GGGAGACCAG GTTTTCAAGG TCCACCTGGT ACAGAAGGTC CTCGTGGCCC CCCTGGAAAT
    GGAGGTATTA AAGGAGAGAG GGGAAATCCA GGTCCACCTG GGCAACCAGG CTTACCTGGC
    TTGAAAGGAG ATCAAGGACC ACCAGGCCTT CCGGGTAACC CTGGCCGACC AGGTCTTAAT
    GGAATGAAAG GAGATCCTGG TCTCCCTGGT GTTCCAGGAT TCCCAGGAAT GAAAGGACCC
    ATTGGAGTAC CAGGCTCAAC TGGCCCTGAT GGGGAACCAG GTCTTACGGG GCCCCCAGGT
    CCTCCTGGGT TGCCTGGTCC TTCAGGACAG AGTATTGTAA TCAAAGGAGA TCCTGGACCT
    CCAGGAATTC CTGGACAACC TGGATTAAAA GGTCTACCAG GACTTCCAGG CCCTCAAGGT
    CTACCAGGTC CAATTGGCCC TCCAGGAGAT CCAGGACGCA ATGGACTCCC AGGCTTTGAT
    GGTGCAGGAG GGCGCAAAGG AGACCCAGGC CTGCCGGGGC AGCCAGGTAG CCGTGGATTG
    GATGGTCCCC CAGGACCAGA TGGATTGCAA GGACCTCCAG GGCCCCCGGG AACCACCTCT
    GTTGCCCATG GATTCCTCAT CACACGTCAC AGTCAGACAA CAGATGCACC ACAATGCCCA
    CAGGGAACAG TCCATATCTA TGATGGCTTT TCTCTCTTAT ATGTACAAGG AAATAAAAGA
    GCCCATGGTC AAGACTTGGG GACAGCTGGC AGCTGCCTTC GTCGCTTCAG TACCATGCCT
    TTCATGTTCT GCAACATTAA CAACGTTTGT AACTTTGCTT CAAGAAATGA CTATTCTTAC
    TGGCTTTCCA CCCCAGAGCC CATGCCAATG AACATGGAAC CCCTGAAGGG CCAGAGCATC
    CAGCCATTCA TTAGTCGATG TGCAGTATGT GAGGCACCAG CTGTGGTGAT TGCAGTTCAC
    AGCCAGACTA TTCAGATTCC TCACTGTCCT CAGGGGTGGG ACTCTCTGTG GATTGGCTAT
    TCATTCATGA TGCATACAAG TGCAGGAGCA GAAGGCTCTG GCCAAGCCCT AGCCTCTCCT
    GGCTCCTGTT TGGAAGAATT TCGTTCAGCT CCCTTCATCG AATGTCATGG ACGAGGGACA
    TGCAATTACT ATGCCAATTC TTACAGCTTT TGGCTGGCCA CTGTTGATAT GTCAGACATG
    TTCAGTAAAC CTCAGTCAGA GACACTGAAA GCAGGCGACT TGAGGACACG TATTAGCCGA
    TGTCAAGTAT GCATGAAAAG GACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.