col4a5 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following col4a5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the col4a5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa65394 XM_004916865.4
Latest version!
Xenopus tropicalis collagen, type IV, alpha 5 (col4a5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa65395 XM_002931817.5
Latest version!
Xenopus tropicalis collagen, type IV, alpha 5 (col4a5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa65396 XM_004916867.4
Latest version!
Xenopus tropicalis collagen, type IV, alpha 5 (col4a5), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa65397 XM_004916868.4
Latest version!
Xenopus tropicalis collagen, type IV, alpha 5 (col4a5), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa65398 XM_004916869.4
Latest version!
Xenopus tropicalis collagen, type IV, alpha 5 (col4a5), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OXa14360 XM_004916865.3
Latest version!
Xenopus tropicalis collagen, type IV, alpha 5 (col4a5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OXa14361 XM_002931817.4
Latest version!
Xenopus tropicalis collagen, type IV, alpha 5 (col4a5), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OXa14362 XM_004916867.3
Latest version!
Xenopus tropicalis collagen, type IV, alpha 5 (col4a5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OXa14363 XM_004916868.3
Latest version!
Xenopus tropicalis collagen, type IV, alpha 5 (col4a5), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OXa14364 XM_004916869.3
Latest version!
Xenopus tropicalis collagen, type IV, alpha 5 (col4a5), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa65394
    Clone ID Related Accession (Same CDS sequence) XM_004916865.4
    Accession Version XM_004916865.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5076bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product collagen alpha-5(IV) chain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004916865.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAGACTGG GAAGTTGGCA GCGGATGCCC TGCCTGGCTG TACTGGTGTT GGTGCTGGGG 
    CACTGGGCAC AGAATGCAGA GTCTGCGGCC TGTCAAGGTT GTACATCGGT ATCGAAATGT
    GACTGCAGTG GTGTTAAAGG AGAAAAGGGT GAGAGAGGCC TTCCAGGGTT AGAAGGACAC
    CTTGGTCTAC CAGGATTTCC TGGACCTGAA GGACCGCCTG GACCTCGTGG TCCCAAGGGT
    GATTCTGGTT TATCAGGGTC TCCAGGACCC AAAGGAATAC GAGGACCTCC TGGACCACCC
    GGATTTCCAG GAAACCCTGG CCAGCCTGGC CTGCCGGGAC ATGATGGTGA ACCAGGGCCT
    GCAGGTATCC CAGGATGCAA TGGCACAAAG GGAGATCGTG GTTTCCCAGG AGGTCCAGGT
    TTTTCGGGTA CACCAGGAAT ACCAGGTCCC CCAGGATTAC CAGGTGTGAA AGGAGATCCA
    GGTGAATCTT TCCCTTTAGC ACCGGGACAA AAAGGTGATC CAGGATTACC AGGTCTTCCA
    GGTTTACCAG GTCGGACTGG TCCTATTGGA GTTCAAGGTC CTGTGGGTCG ACCTGGTCCT
    GCTGGCTTAC CGGGGTCACC AGGAGTACCT GGACCTCCAG GACCCAAGGG TAACATGGGG
    CTGAATTTCC AGGGATCAAA GGGAGAAAAG GGTGAACCTG GTATTCAAGG ACTACCAGGA
    CCACCGGGAC AACTCGAGCA AGTTGAACCT CCACAAACTA CAATTCAGAA GGGAGAGAAG
    GGTATCCCCG GTGACCAAGG ATTTCCAGGA GAGCGTGGGC CAATGGGTCC TCCTGGTCCT
    CCTGGAGGTG CAAAGGGAGA GAAGGGAGAA CCAGGGGAAC CTGGAAAGAT TGGGAAGCCT
    GGAAAAGATG GAAAGATTGG TCTGCCCGGT GGAGAGGGTT TCAGGGGTGA CCCAGGCTTG
    CCGGGAGGTC CAGGCAGAGA TGGGGACAAG GGTCAAAAAG GTGAAAATGG GCTCCCTGGG
    CCACCAGGAG AGGTAATTTC AAGACCAGGG ACAGGCAGAG TTGGACCAAA AGGAAACACA
    GGACCCCCTG GAATAACAGG GCCAAAAGGA GACCGTGGAT TCCCTGGATT ACAAGGTCCC
    CCTGGTTTTC CTGGAACACC AGGTACTTCA AGTGTAGGCC CATCAGGAAA TCCAGGCCTT
    CCTGGTGAAA GAGGGCAAAA AGGCGATGAT GGTCCACCAG GAATCTCTAT TCCAGGGCTT
    CCAGGTTCTG AAGGTCTTGC AGGACCCCCT GGTCCCCCAG GTTTGCCTGG TCGACCTGGA
    ACATCTGCAG TTGGTCCTGC AATGGTATGC CAGCCAGGGC TACCTGGTAC TCAAGGACTT
    CCAGGAGATC CAGGATTTTT AGGAGAAAGA GGACAGAAAG GTGACAAAGG AGACACATGT
    TATAACTGCA TAGGAAATGG TATTTTAGGT CCGAAGGGGG ATCCTGGTTT GCCTGGTTTC
    CCTGGAAGTC CAGGTTCACC TGGAGCTCCA GGGCAAAAAG GAGCAATGGG TATCCCAGGA
    ACAAATGGGC TTCCAGGACC TCCGGGTTTT CCTGGCAATC CTGGTATCTC TGGCCGACCC
    GGTCCCAAAG GAGAACCTGG AGAAGTAATT GGCGAAATTA AAGGAGAAAA GGGGGACCCT
    GGATTCCCAG GTCCAGCTGG ATCTCCTGGT CTGGATGGTG TCCCTGGAAG AGACGGAACC
    CCAGGGATCC CTGGCCCTAA GGGAGAGCCG GGTGGCTTCG TGTTGAAAGG CGATCGTGGT
    TTTCCTGGTG ACATTGGGTT CCCTGGACAT CCTGGAGATC GAGGACTAAT GGGTCCTCCT
    GGTTTTGGTC CCAGTGGTCC ACCTGGAGAG AAGGGAATTC AAGGTGTTTC TGGTCGTCCA
    GGAGCCCCTG GAATACCTGG CCCTAAAGGA TCTCCTGGAC AGACTGTGAT GGAAAAAGGG
    GATTTTGGAC CCCCTGGTCT CCCAGGTATC CCAGGGGATG CCGGAACTCC AGGTGAACCA
    GGTCTACCTG GTCAGCCAGG AATTCCAGGT GTTCCAGGTT CAAAAGGAGA ACCAGGCATT
    CCAGGCATTG GACTTCCTGG GTCTGCAGGC GCAAAAGGAT TTCCTGGTAA TACTGGGCCA
    CCAGGTGCAC CAGGGTTGGC AGGAAGACCT GGTTCAGATG GGTCACCAGG ACAGCCTGGC
    TTGCCAGGAC CAAAGGGTGA CCCTGGGTTT GGTTTACCTG GGCTACCAGG CTCCCCAGGC
    CCTGCTGGAT TGAAAGGATC CCAAGGACCA AAAGGAGAAT CAGGCTTCCC AGGTTCTCCA
    GGCCTTCCTG GACGAGCAGG ATTTGATGGG CCTGTGGGAC CAAAAGGTGA CTCTGGACAA
    AATGGCTTAC CAGGAACCCC TGGACCACCT GGGGTACCTG GTGCTGGTGG TCAAGGCCCT
    CCAGGACAAC CAGGGCTTCC AGGGCCAGCA GGCCCTCCAG GTCCCACAGG GAACACTGGA
    ATACAGGGAG AGAAAGGAGA TCCTGGTGCT CCAGGGTTTG ACATGCCAGG CCCACCAGGA
    GATCGTGGTA GCCCTGGATT TCCAGGAGAA AGAGGGTTTA CAGGTCCACC AGGGCCACCT
    GGAGCCCAAG GTCGCGATGG ATTACCAGGC TTCTCAGGTG CTAAAGGTGA AATGGGTGTA
    ATGGGACCAC CAGGATCTGT CGGACCAGCT GGTAATCCTG GAAACCCTGG TTTACCTGGG
    TCTAAAGGAA ATGATGGGAA TCCCGGACAA CCTGGCCTGT CTGGTTTACC AGGGGCTGAA
    GGTGAAAAGG GAGAGAGAGG ATTACCAGGA CCCCCTGGAA GAGTTGATTC TGCAAACCTA
    CAGTTTAAAG GAGTTAAAGG AGAACCTGGC TTGCCAGGTT CATCAGGACT AAGTGGGGCA
    AAAGGTGCTG CTGGACTTCC TGGTAACTCA GGATCCCCAG GGCAGATTGG AATTCCAGGA
    TCTCCAGGTC TTCCAGGGCT CAAGGGAGAT TCTGGCCTTC CTGGTCAGCC GGGTACTTCA
    GGTCCTCCTG GATTAAAAGG GAGCATGGGA GAGATGGGAC TTCCTGGGCC TCCTGGTTCT
    AAAGGTTCCC CTGGACAGCC AGGAAGGCCT GGTTCAGCAG GGTCTCCTGG GTTTTCTGGA
    CTGAAAGGTG AAAAAGGTGA ACCAGGAATT TCAGGAGCTG GTATCCCAGG CTTTCCTGGG
    CAGAAGGGTG AACCAGGGCA AAATGGCTTT CCAGGCAGCC CTGGTAGTAA AGGCAACCCA
    GGAAACCCAG GACTGCCCGG CCTACCAGGG ATTTCAGGAC CTAAAGGGGA TCCAGGACTT
    CCAGGATTCC CAGGCAGCCC TGGATCTCCA GGCCTTAAAG GAGTTGATGG GCCCACAGGA
    AACTCTGGGT TACCCGGATC TCCAGGCTTA CGGGGTGAAT CAGGTCGTCC CGGACCTCAA
    GGAACTCCTG GTGAGAAGGG TCAACCAGGT CAGGATGGAA TTCCTGGGCC TTCTGGTTTG
    AAGGGAGAAC CAGGTTTGCC AGGTTTTGGA TCCCCAGGGC TGCCTGGCCT TCCAGGAAGT
    CAAGGTCAAA AGGGTGATGC AGGACTTTCT GGAAGCCCTG GAAACCCCGG ACTTCCAGGG
    TTCAAAGGAG ATCAAGGCTT TCCAGGATCT CCTGGGCCAC AAGGACCAAC AGGACAACCT
    GGCCTACCAG GGCCACGTAC AGAAGGACCC AAGGGAGACC CTGGGCCTGT AGGTCCCCCT
    GGAAGACCAG GCCCTCAAGG TTATCCAGGT CCTCCTGGCT CAGAAGGTCC ACGTGGTCCC
    CCTGGTGATG GAGGCTTTAA AGGAGAAAAA GGAAACCCTG GATTTCCAGG TCAGCCTGGA
    CTTACAGGAT CTAAAGGAGA ACATGGAGCA TCTGGTTTCC CAGGTGACCC AGGACGACCA
    GGTCTTAATG GGATGAAAGG TGATCAAGGT GTTCCTGGTG TTCCAGGATT CCCAGGTATG
    AAAGGACCAG GTGGCCCTCC TGGATTGGCT GGCCTTAAAG GTGAATCCGG ACTCCCAGGT
    GCTATAGGTT TGCCAGGTCT TCCTGGGCCT AAAGGAGAAT ACCGTGAGAT AAAAGGAGCT
    CCAGGTCTGC CAGGTCCTCC AGGTCGCCCA GGAGTAAATG GACCTCCAGG ACCCCCCGGA
    TCACCAGGAC TTCCAGGTTC TTTGGGTCTC CCTGGTGATA CTGGCAGAGA TGGACTCCCT
    GGATTTGAGG GACAACTTGG ACGAAAGGGT GACCGAGGCC TACCAGGCCA ACCAGGATCT
    CGTGGAATTA ATGGCCCACC TGGCCCAGAT GGTTTACAAG GCCCCCCTGG CCCCCCTGGT
    CTAGGATCTG TTGCTCATGG ATTCCTTATA ACCCGACACA GTCAGTCAAC AGATGCCCCT
    GGCTGTCCTT TTGGAACCAC CAGGATCTAT GATGGCTATT CACTACTTTA TGTGCAAGGA
    AATGAGAGGG CACACGGACA AGATTTGGGT ACCGCTGGCA GCTGCCTCCG TCGCTTCAGC
    ACAATGCCTT TCATGTTCTG CAACATTAAT AACGTGTGTA ACTTTGCTTC AAGAAATGAC
    TACTCCTACT GGTTGTCCAC GCCTGAGCCT ATGCCTATGA GCATGGAACC TTTAAGAGGG
    AGGGATATCC AACCATTCAT TAGCAGGTGT GCGGTCTGTG AAGCGCCAGC CATGGTGATT
    GCAGTTCACA GCCAGACAAT ACAAATCCCA CAGTGCCCAC AAGGCTGGTC CTCTCTCTGG
    ATTGGATACT CTTTCATGAT GCATACAAGT GCCGGTGCTG AAGGCTCAGG ACAAGCTCTG
    GCATCGCCTG GATCGTGCTT GGAGGAATTC CGATCTGCAC CTTTCATTGA ATGTCATGGC
    CGGGGAACCT GCAATTACTA TGCCAATTCA TACAGTTTTT GGTTGGCCAC AGTGGAAATG
    TCTGAAATGT TTAGCAAACC TCAGTCAGAA ACACTAAAAG CGGGTGATCT AAGGACACGC
    GTCAGTCGCT GCCAAGTTTG TATGAAGAGG ACGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004916922.2
    CDS281..5356
    Translation

    Target ORF information:

    RefSeq Version XM_004916865.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis collagen, type IV, alpha 5 (col4a5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004916865.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAGACTGG GAAGTTGGCA GCGGATGCCC TGCCTGGCTG TACTGGTGTT GGTGCTGGGG 
    CACTGGGCAC AGAATGCAGA GTCTGCGGCC TGTCAAGGTT GTACATCGGT ATCGAAATGT
    GACTGCAGTG GTGTTAAAGG AGAAAAGGGT GAGAGAGGCC TTCCAGGGTT AGAAGGACAC
    CTTGGTCTAC CAGGATTTCC TGGACCTGAA GGACCGCCTG GACCTCGTGG TCCCAAGGGT
    GATTCTGGTT TATCAGGGTC TCCAGGACCC AAAGGAATAC GAGGACCTCC TGGACCACCC
    GGATTTCCAG GAAACCCTGG CCAGCCTGGC CTGCCGGGAC ATGATGGTGA ACCAGGGCCT
    GCAGGTATCC CAGGATGCAA TGGCACAAAG GGAGATCGTG GTTTCCCAGG AGGTCCAGGT
    TTTTCGGGTA CACCAGGAAT ACCAGGTCCC CCAGGATTAC CAGGTGTGAA AGGAGATCCA
    GGTGAATCTT TCCCTTTAGC ACCGGGACAA AAAGGTGATC CAGGATTACC AGGTCTTCCA
    GGTTTACCAG GTCGGACTGG TCCTATTGGA GTTCAAGGTC CTGTGGGTCG ACCTGGTCCT
    GCTGGCTTAC CGGGGTCACC AGGAGTACCT GGACCTCCAG GACCCAAGGG TAACATGGGG
    CTGAATTTCC AGGGATCAAA GGGAGAAAAG GGTGAACCTG GTATTCAAGG ACTACCAGGA
    CCACCGGGAC AACTCGAGCA AGTTGAACCT CCACAAACTA CAATTCAGAA GGGAGAGAAG
    GGTATCCCCG GTGACCAAGG ATTTCCAGGA GAGCGTGGGC CAATGGGTCC TCCTGGTCCT
    CCTGGAGGTG CAAAGGGAGA GAAGGGAGAA CCAGGGGAAC CTGGAAAGAT TGGGAAGCCT
    GGAAAAGATG GAAAGATTGG TCTGCCCGGT GGAGAGGGTT TCAGGGGTGA CCCAGGCTTG
    CCGGGAGGTC CAGGCAGAGA TGGGGACAAG GGTCAAAAAG GTGAAAATGG GCTCCCTGGG
    CCACCAGGAG AGGTAATTTC AAGACCAGGG ACAGGCAGAG TTGGACCAAA AGGAAACACA
    GGACCCCCTG GAATAACAGG GCCAAAAGGA GACCGTGGAT TCCCTGGATT ACAAGGTCCC
    CCTGGTTTTC CTGGAACACC AGGTACTTCA AGTGTAGGCC CATCAGGAAA TCCAGGCCTT
    CCTGGTGAAA GAGGGCAAAA AGGCGATGAT GGTCCACCAG GAATCTCTAT TCCAGGGCTT
    CCAGGTTCTG AAGGTCTTGC AGGACCCCCT GGTCCCCCAG GTTTGCCTGG TCGACCTGGA
    ACATCTGCAG TTGGTCCTGC AATGGTATGC CAGCCAGGGC TACCTGGTAC TCAAGGACTT
    CCAGGAGATC CAGGATTTTT AGGAGAAAGA GGACAGAAAG GTGACAAAGG AGACACATGT
    TATAACTGCA TAGGAAATGG TATTTTAGGT CCGAAGGGGG ATCCTGGTTT GCCTGGTTTC
    CCTGGAAGTC CAGGTTCACC TGGAGCTCCA GGGCAAAAAG GAGCAATGGG TATCCCAGGA
    ACAAATGGGC TTCCAGGACC TCCGGGTTTT CCTGGCAATC CTGGTATCTC TGGCCGACCC
    GGTCCCAAAG GAGAACCTGG AGAAGTAATT GGCGAAATTA AAGGAGAAAA GGGGGACCCT
    GGATTCCCAG GTCCAGCTGG ATCTCCTGGT CTGGATGGTG TCCCTGGAAG AGACGGAACC
    CCAGGGATCC CTGGCCCTAA GGGAGAGCCG GGTGGCTTCG TGTTGAAAGG CGATCGTGGT
    TTTCCTGGTG ACATTGGGTT CCCTGGACAT CCTGGAGATC GAGGACTAAT GGGTCCTCCT
    GGTTTTGGTC CCAGTGGTCC ACCTGGAGAG AAGGGAATTC AAGGTGTTTC TGGTCGTCCA
    GGAGCCCCTG GAATACCTGG CCCTAAAGGA TCTCCTGGAC AGACTGTGAT GGAAAAAGGG
    GATTTTGGAC CCCCTGGTCT CCCAGGTATC CCAGGGGATG CCGGAACTCC AGGTGAACCA
    GGTCTACCTG GTCAGCCAGG AATTCCAGGT GTTCCAGGTT CAAAAGGAGA ACCAGGCATT
    CCAGGCATTG GACTTCCTGG GTCTGCAGGC GCAAAAGGAT TTCCTGGTAA TACTGGGCCA
    CCAGGTGCAC CAGGGTTGGC AGGAAGACCT GGTTCAGATG GGTCACCAGG ACAGCCTGGC
    TTGCCAGGAC CAAAGGGTGA CCCTGGGTTT GGTTTACCTG GGCTACCAGG CTCCCCAGGC
    CCTGCTGGAT TGAAAGGATC CCAAGGACCA AAAGGAGAAT CAGGCTTCCC AGGTTCTCCA
    GGCCTTCCTG GACGAGCAGG ATTTGATGGG CCTGTGGGAC CAAAAGGTGA CTCTGGACAA
    AATGGCTTAC CAGGAACCCC TGGACCACCT GGGGTACCTG GTGCTGGTGG TCAAGGCCCT
    CCAGGACAAC CAGGGCTTCC AGGGCCAGCA GGCCCTCCAG GTCCCACAGG GAACACTGGA
    ATACAGGGAG AGAAAGGAGA TCCTGGTGCT CCAGGGTTTG ACATGCCAGG CCCACCAGGA
    GATCGTGGTA GCCCTGGATT TCCAGGAGAA AGAGGGTTTA CAGGTCCACC AGGGCCACCT
    GGAGCCCAAG GTCGCGATGG ATTACCAGGC TTCTCAGGTG CTAAAGGTGA AATGGGTGTA
    ATGGGACCAC CAGGATCTGT CGGACCAGCT GGTAATCCTG GAAACCCTGG TTTACCTGGG
    TCTAAAGGAA ATGATGGGAA TCCCGGACAA CCTGGCCTGT CTGGTTTACC AGGGGCTGAA
    GGTGAAAAGG GAGAGAGAGG ATTACCAGGA CCCCCTGGAA GAGTTGATTC TGCAAACCTA
    CAGTTTAAAG GAGTTAAAGG AGAACCTGGC TTGCCAGGTT CATCAGGACT AAGTGGGGCA
    AAAGGTGCTG CTGGACTTCC TGGTAACTCA GGATCCCCAG GGCAGATTGG AATTCCAGGA
    TCTCCAGGTC TTCCAGGGCT CAAGGGAGAT TCTGGCCTTC CTGGTCAGCC GGGTACTTCA
    GGTCCTCCTG GATTAAAAGG GAGCATGGGA GAGATGGGAC TTCCTGGGCC TCCTGGTTCT
    AAAGGTTCCC CTGGACAGCC AGGAAGGCCT GGTTCAGCAG GGTCTCCTGG GTTTTCTGGA
    CTGAAAGGTG AAAAAGGTGA ACCAGGAATT TCAGGAGCTG GTATCCCAGG CTTTCCTGGG
    CAGAAGGGTG AACCAGGGCA AAATGGCTTT CCAGGCAGCC CTGGTAGTAA AGGCAACCCA
    GGAAACCCAG GACTGCCCGG CCTACCAGGG ATTTCAGGAC CTAAAGGGGA TCCAGGACTT
    CCAGGATTCC CAGGCAGCCC TGGATCTCCA GGCCTTAAAG GAGTTGATGG GCCCACAGGA
    AACTCTGGGT TACCCGGATC TCCAGGCTTA CGGGGTGAAT CAGGTCGTCC CGGACCTCAA
    GGAACTCCTG GTGAGAAGGG TCAACCAGGT CAGGATGGAA TTCCTGGGCC TTCTGGTTTG
    AAGGGAGAAC CAGGTTTGCC AGGTTTTGGA TCCCCAGGGC TGCCTGGCCT TCCAGGAAGT
    CAAGGTCAAA AGGGTGATGC AGGACTTTCT GGAAGCCCTG GAAACCCCGG ACTTCCAGGG
    TTCAAAGGAG ATCAAGGCTT TCCAGGATCT CCTGGGCCAC AAGGACCAAC AGGACAACCT
    GGCCTACCAG GGCCACGTAC AGAAGGACCC AAGGGAGACC CTGGGCCTGT AGGTCCCCCT
    GGAAGACCAG GCCCTCAAGG TTATCCAGGT CCTCCTGGCT CAGAAGGTCC ACGTGGTCCC
    CCTGGTGATG GAGGCTTTAA AGGAGAAAAA GGAAACCCTG GATTTCCAGG TCAGCCTGGA
    CTTACAGGAT CTAAAGGAGA ACATGGAGCA TCTGGTTTCC CAGGTGACCC AGGACGACCA
    GGTCTTAATG GGATGAAAGG TGATCAAGGT GTTCCTGGTG TTCCAGGATT CCCAGGTATG
    AAAGGACCAG GTGGCCCTCC TGGATTGGCT GGCCTTAAAG GTGAATCCGG ACTCCCAGGT
    GCTATAGGTT TGCCAGGTCT TCCTGGGCCT AAAGGAGAAT ACCGTGAGAT AAAAGGAGCT
    CCAGGTCTGC CAGGTCCTCC AGGTCGCCCA GGAGTAAATG GACCTCCAGG ACCCCCCGGA
    TCACCAGGAC TTCCAGGTTC TTTGGGTCTC CCTGGTGATA CTGGCAGAGA TGGACTCCCT
    GGATTTGAGG GACAACTTGG ACGAAAGGGT GACCGAGGCC TACCAGGCCA ACCAGGATCT
    CGTGGAATTA ATGGCCCACC TGGCCCAGAT GGTTTACAAG GCCCCCCTGG CCCCCCTGGT
    CTAGGATCTG TTGCTCATGG ATTCCTTATA ACCCGACACA GTCAGTCAAC AGATGCCCCT
    GGCTGTCCTT TTGGAACCAC CAGGATCTAT GATGGCTATT CACTACTTTA TGTGCAAGGA
    AATGAGAGGG CACACGGACA AGATTTGGGT ACCGCTGGCA GCTGCCTCCG TCGCTTCAGC
    ACAATGCCTT TCATGTTCTG CAACATTAAT AACGTGTGTA ACTTTGCTTC AAGAAATGAC
    TACTCCTACT GGTTGTCCAC GCCTGAGCCT ATGCCTATGA GCATGGAACC TTTAAGAGGG
    AGGGATATCC AACCATTCAT TAGCAGGTGT GCGGTCTGTG AAGCGCCAGC CATGGTGATT
    GCAGTTCACA GCCAGACAAT ACAAATCCCA CAGTGCCCAC AAGGCTGGTC CTCTCTCTGG
    ATTGGATACT CTTTCATGAT GCATACAAGT GCCGGTGCTG AAGGCTCAGG ACAAGCTCTG
    GCATCGCCTG GATCGTGCTT GGAGGAATTC CGATCTGCAC CTTTCATTGA ATGTCATGGC
    CGGGGAACCT GCAATTACTA TGCCAATTCA TACAGTTTTT GGTTGGCCAC AGTGGAAATG
    TCTGAAATGT TTAGCAAACC TCAGTCAGAA ACACTAAAAG CGGGTGATCT AAGGACACGC
    GTCAGTCGCT GCCAAGTTTG TATGAAGAGG ACGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa65395
    Clone ID Related Accession (Same CDS sequence) XM_002931817.5
    Accession Version XM_002931817.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5067bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product collagen alpha-5(IV) chain isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002931817.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAGACTGG GAAGTTGGCA GCGGATGCCC TGCCTGGCTG TACTGGTGTT GGTGCTGGGG 
    CACTGGGCAC AGAATGCAGA GTCTGCGGCC TGTCAAGGTT GTACATCGGT ATCGAAATGT
    GACTGCAGTG GTGTTAAAGG AGAAAAGGGT GAGAGAGGCC TTCCAGGGTT AGAAGGACAC
    CTTGGTCTAC CAGGATTTCC TGGACCTGAA GGACCGCCTG GACCTCGTGG TCCCAAGGGT
    GATTCTGGTT TATCAGGGTC TCCAGGACCC AAAGGAATAC GAGGACCTCC TGGACCACCC
    GGATTTCCAG GAAACCCTGG CCAGCCTGGC CTGCCGGGAC ATGATGGTGA ACCAGGGCCT
    GCAGGTATCC CAGGATGCAA TGGCACAAAG GGAGATCGTG GTTTCCCAGG AGGTCCAGGT
    TTTTCGGGTA CACCAGGAAT ACCAGGTCCC CCAGGATTAC CAGGTGTGAA AGGAGATCCA
    GGTGAATCTT TCCCTTTAGC ACCGGGACAA AAAGGTGATC CAGGATTACC AGGTCTTCCA
    GGTTTACCAG GTCGGACTGG TCCTATTGGA GTTCAAGGTC CTGTGGGTCG ACCTGGTCCT
    GCTGGCTTAC CGGGGTCACC AGGAGTACCT GGACCTCCAG GACCCAAGGG TAACATGGGG
    CTGAATTTCC AGGGATCAAA GGGAGAAAAG GGTGAACCTG GTATTCAAGG ACTACCAGGA
    CCACCGGGAC AACTCGAGCA AGTTGAACCT CCACAAACTA CAATTCAGAA GGGAGAGAAG
    GGTATCCCCG GTGACCAAGG ATTTCCAGGA GAGCGTGGGC CAATGGGTCC TCCTGGTCCT
    CCTGGAGGTG CAAAGGGAGA GAAGGGAGAA CCAGGGGAAC CTGGAAAGAT TGGGAAGCCT
    GGAAAAGATG GAAAGATTGG TCTGCCCGGT GGAGAGGGTT TCAGGGGTGA CCCAGGCTTG
    CCGGGAGGTC CAGGCAGAGA TGGGGACAAG GGTCAAAAAG GTGAAAATGG GCTCCCTGGG
    CCACCAGGAG AGGTAATTTC AAGACCAGGG ACAGGCAGAG TTGGACCAAA AGGAAACACA
    GGACCCCCTG GAATAACAGG GCCAAAAGGA GACCGTGGAT TCCCTGGATT ACAAGGTCCC
    CCTGGTTTTC CTGGAACACC AGGTACTTCA AGTGTAGGCC CATCAGGAAA TCCAGGCCTT
    CCTGGTGAAA GAGGGCAAAA AGGCGATGAT GGTCCACCAG GAATCTCTAT TCCAGGGCTT
    CCAGGTTCTG AAGGTCTTGC AGGACCCCCT GGTCCCCCAG GTTTGCCTGG TCGACCTGGA
    ACATCTGCAG TTGGTCCTGC AATGGTATGC CAGCCAGGGC TACCTGGTAC TCAAGGACTT
    CCAGGAGATC CAGGATTTTT AGGAGAAAGA GGACAGAAAG GTGACAAAGG AGACACATGT
    TATAACTGCA TAGGAAATGG TATTTTAGGT CCGAAGGGGG ATCCTGGTTT GCCTGGTTTC
    CCTGGAAGTC CAGGTTCACC TGGAGCTCCA GGGCAAAAAG GAGCAATGGG TATCCCAGGA
    ACAAATGGGC TTCCAGGACC TCCGGGTTTT CCTGGCAATC CTGGTATCTC TGGCCGACCC
    GGTCCCAAAG GAGAACCTGG AGAAGTAATT GGCGAAATTA AAGGAGAAAA GGGGGACCCT
    GGATTCCCAG GTCCAGCTGG ATCTCCTGGT CTGGATGGTG TCCCTGGAAG AGACGGAACC
    CCAGGGATCC CTGGCCCTAA GGGAGAGCCG GGTGGCTTCG TGTTGAAAGG CGATCGTGGT
    TTTCCTGGTG ACATTGGGTT CCCTGGACAT CCTGGAGATC GAGGACTAAT GGGTCCTCCT
    GGTTTTGGTC CCAGTGGTCC ACCTGGAGAG AAGGGAATTC AAGGTGTTTC TGGTCGTCCA
    GGAGCCCCTG GAATACCTGG CCCTAAAGGA TCTCCTGGAC AGACTGTGAT GGAAAAAGGG
    GATTTTGGAC CCCCTGGTCT CCCAGGTATC CCAGGGGATG CCGGAACTCC AGGTGAACCA
    GGTCTACCTG GTCAGCCAGG AATTCCAGGT GTTCCAGGTT CAAAAGGAGA ACCAGGCATT
    CCAGGCATTG GACTTCCTGG GTCTGCAGGC GCAAAAGGAT TTCCTGGTAA TACTGGGCCA
    CCAGGTGCAC CAGGGTTGGC AGGAAGACCT GGTTCAGATG GGTCACCAGG ACAGCCTGGC
    TTGCCAGGAC CAAAGGGTGA CCCTGGGTTT GGTTTACCTG GGCTACCAGG CTCCCCAGGC
    CCTGCTGGAT TGAAAGGATC CCAAGGACCA AAAGGAGAAT CAGGCTTCCC AGGTTCTCCA
    GGCCTTCCTG GACGAGCAGG ATTTGATGGG CCTGTGGGAC CAAAAGGTGA CTCTGGACAA
    AATGGCTTAC CAGGAACCCC TGGACCACCT GGGGTACCTG GTGCTGGTGG TCAAGGCCCT
    CCAGGACAAC CAGGGCTTCC AGGGCCAGCA GGCCCTCCAG GGAACACTGG AATACAGGGA
    GAGAAAGGAG ATCCTGGTGC TCCAGGGTTT GACATGCCAG GCCCACCAGG AGATCGTGGT
    AGCCCTGGAT TTCCAGGAGA AAGAGGGTTT ACAGGTCCAC CAGGGCCACC TGGAGCCCAA
    GGTCGCGATG GATTACCAGG CTTCTCAGGT GCTAAAGGTG AAATGGGTGT AATGGGACCA
    CCAGGATCTG TCGGACCAGC TGGTAATCCT GGAAACCCTG GTTTACCTGG GTCTAAAGGA
    AATGATGGGA ATCCCGGACA ACCTGGCCTG TCTGGTTTAC CAGGGGCTGA AGGTGAAAAG
    GGAGAGAGAG GATTACCAGG ACCCCCTGGA AGAGTTGATT CTGCAAACCT ACAGTTTAAA
    GGAGTTAAAG GAGAACCTGG CTTGCCAGGT TCATCAGGAC TAAGTGGGGC AAAAGGTGCT
    GCTGGACTTC CTGGTAACTC AGGATCCCCA GGGCAGATTG GAATTCCAGG ATCTCCAGGT
    CTTCCAGGGC TCAAGGGAGA TTCTGGCCTT CCTGGTCAGC CGGGTACTTC AGGTCCTCCT
    GGATTAAAAG GGAGCATGGG AGAGATGGGA CTTCCTGGGC CTCCTGGTTC TAAAGGTTCC
    CCTGGACAGC CAGGAAGGCC TGGTTCAGCA GGGTCTCCTG GGTTTTCTGG ACTGAAAGGT
    GAAAAAGGTG AACCAGGAAT TTCAGGAGCT GGTATCCCAG GCTTTCCTGG GCAGAAGGGT
    GAACCAGGGC AAAATGGCTT TCCAGGCAGC CCTGGTAGTA AAGGCAACCC AGGAAACCCA
    GGACTGCCCG GCCTACCAGG GATTTCAGGA CCTAAAGGGG ATCCAGGACT TCCAGGATTC
    CCAGGCAGCC CTGGATCTCC AGGCCTTAAA GGAGTTGATG GGCCCACAGG AAACTCTGGG
    TTACCCGGAT CTCCAGGCTT ACGGGGTGAA TCAGGTCGTC CCGGACCTCA AGGAACTCCT
    GGTGAGAAGG GTCAACCAGG TCAGGATGGA ATTCCTGGGC CTTCTGGTTT GAAGGGAGAA
    CCAGGTTTGC CAGGTTTTGG ATCCCCAGGG CTGCCTGGCC TTCCAGGAAG TCAAGGTCAA
    AAGGGTGATG CAGGACTTTC TGGAAGCCCT GGAAACCCCG GACTTCCAGG GTTCAAAGGA
    GATCAAGGCT TTCCAGGATC TCCTGGGCCA CAAGGACCAA CAGGACAACC TGGCCTACCA
    GGGCCACGTA CAGAAGGACC CAAGGGAGAC CCTGGGCCTG TAGGTCCCCC TGGAAGACCA
    GGCCCTCAAG GTTATCCAGG TCCTCCTGGC TCAGAAGGTC CACGTGGTCC CCCTGGTGAT
    GGAGGCTTTA AAGGAGAAAA AGGAAACCCT GGATTTCCAG GTCAGCCTGG ACTTACAGGA
    TCTAAAGGAG AACATGGAGC ATCTGGTTTC CCAGGTGACC CAGGACGACC AGGTCTTAAT
    GGGATGAAAG GTGATCAAGG TGTTCCTGGT GTTCCAGGAT TCCCAGGTAT GAAAGGACCA
    GGTGGCCCTC CTGGATTGGC TGGCCTTAAA GGTGAATCCG GACTCCCAGG TGCTATAGGT
    TTGCCAGGTC TTCCTGGGCC TAAAGGAGAA TACCGTGAGA TAAAAGGAGC TCCAGGTCTG
    CCAGGTCCTC CAGGTCGCCC AGGAGTAAAT GGACCTCCAG GACCCCCCGG ATCACCAGGA
    CTTCCAGGTT CTTTGGGTCT CCCTGGTGAT ACTGGCAGAG ATGGACTCCC TGGATTTGAG
    GGACAACTTG GACGAAAGGG TGACCGAGGC CTACCAGGCC AACCAGGATC TCGTGGAATT
    AATGGCCCAC CTGGCCCAGA TGGTTTACAA GGCCCCCCTG GCCCCCCTGG TCTAGGATCT
    GTTGCTCATG GATTCCTTAT AACCCGACAC AGTCAGTCAA CAGATGCCCC TGGCTGTCCT
    TTTGGAACCA CCAGGATCTA TGATGGCTAT TCACTACTTT ATGTGCAAGG AAATGAGAGG
    GCACACGGAC AAGATTTGGG TACCGCTGGC AGCTGCCTCC GTCGCTTCAG CACAATGCCT
    TTCATGTTCT GCAACATTAA TAACGTGTGT AACTTTGCTT CAAGAAATGA CTACTCCTAC
    TGGTTGTCCA CGCCTGAGCC TATGCCTATG AGCATGGAAC CTTTAAGAGG GAGGGATATC
    CAACCATTCA TTAGCAGGTG TGCGGTCTGT GAAGCGCCAG CCATGGTGAT TGCAGTTCAC
    AGCCAGACAA TACAAATCCC ACAGTGCCCA CAAGGCTGGT CCTCTCTCTG GATTGGATAC
    TCTTTCATGA TGCATACAAG TGCCGGTGCT GAAGGCTCAG GACAAGCTCT GGCATCGCCT
    GGATCGTGCT TGGAGGAATT CCGATCTGCA CCTTTCATTG AATGTCATGG CCGGGGAACC
    TGCAATTACT ATGCCAATTC ATACAGTTTT TGGTTGGCCA CAGTGGAAAT GTCTGAAATG
    TTTAGCAAAC CTCAGTCAGA AACACTAAAA GCGGGTGATC TAAGGACACG CGTCAGTCGC
    TGCCAAGTTT GTATGAAGAG GACGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002931863.3
    CDS281..5347
    Translation

    Target ORF information:

    RefSeq Version XM_002931817.5
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis collagen, type IV, alpha 5 (col4a5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002931817.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAGACTGG GAAGTTGGCA GCGGATGCCC TGCCTGGCTG TACTGGTGTT GGTGCTGGGG 
    CACTGGGCAC AGAATGCAGA GTCTGCGGCC TGTCAAGGTT GTACATCGGT ATCGAAATGT
    GACTGCAGTG GTGTTAAAGG AGAAAAGGGT GAGAGAGGCC TTCCAGGGTT AGAAGGACAC
    CTTGGTCTAC CAGGATTTCC TGGACCTGAA GGACCGCCTG GACCTCGTGG TCCCAAGGGT
    GATTCTGGTT TATCAGGGTC TCCAGGACCC AAAGGAATAC GAGGACCTCC TGGACCACCC
    GGATTTCCAG GAAACCCTGG CCAGCCTGGC CTGCCGGGAC ATGATGGTGA ACCAGGGCCT
    GCAGGTATCC CAGGATGCAA TGGCACAAAG GGAGATCGTG GTTTCCCAGG AGGTCCAGGT
    TTTTCGGGTA CACCAGGAAT ACCAGGTCCC CCAGGATTAC CAGGTGTGAA AGGAGATCCA
    GGTGAATCTT TCCCTTTAGC ACCGGGACAA AAAGGTGATC CAGGATTACC AGGTCTTCCA
    GGTTTACCAG GTCGGACTGG TCCTATTGGA GTTCAAGGTC CTGTGGGTCG ACCTGGTCCT
    GCTGGCTTAC CGGGGTCACC AGGAGTACCT GGACCTCCAG GACCCAAGGG TAACATGGGG
    CTGAATTTCC AGGGATCAAA GGGAGAAAAG GGTGAACCTG GTATTCAAGG ACTACCAGGA
    CCACCGGGAC AACTCGAGCA AGTTGAACCT CCACAAACTA CAATTCAGAA GGGAGAGAAG
    GGTATCCCCG GTGACCAAGG ATTTCCAGGA GAGCGTGGGC CAATGGGTCC TCCTGGTCCT
    CCTGGAGGTG CAAAGGGAGA GAAGGGAGAA CCAGGGGAAC CTGGAAAGAT TGGGAAGCCT
    GGAAAAGATG GAAAGATTGG TCTGCCCGGT GGAGAGGGTT TCAGGGGTGA CCCAGGCTTG
    CCGGGAGGTC CAGGCAGAGA TGGGGACAAG GGTCAAAAAG GTGAAAATGG GCTCCCTGGG
    CCACCAGGAG AGGTAATTTC AAGACCAGGG ACAGGCAGAG TTGGACCAAA AGGAAACACA
    GGACCCCCTG GAATAACAGG GCCAAAAGGA GACCGTGGAT TCCCTGGATT ACAAGGTCCC
    CCTGGTTTTC CTGGAACACC AGGTACTTCA AGTGTAGGCC CATCAGGAAA TCCAGGCCTT
    CCTGGTGAAA GAGGGCAAAA AGGCGATGAT GGTCCACCAG GAATCTCTAT TCCAGGGCTT
    CCAGGTTCTG AAGGTCTTGC AGGACCCCCT GGTCCCCCAG GTTTGCCTGG TCGACCTGGA
    ACATCTGCAG TTGGTCCTGC AATGGTATGC CAGCCAGGGC TACCTGGTAC TCAAGGACTT
    CCAGGAGATC CAGGATTTTT AGGAGAAAGA GGACAGAAAG GTGACAAAGG AGACACATGT
    TATAACTGCA TAGGAAATGG TATTTTAGGT CCGAAGGGGG ATCCTGGTTT GCCTGGTTTC
    CCTGGAAGTC CAGGTTCACC TGGAGCTCCA GGGCAAAAAG GAGCAATGGG TATCCCAGGA
    ACAAATGGGC TTCCAGGACC TCCGGGTTTT CCTGGCAATC CTGGTATCTC TGGCCGACCC
    GGTCCCAAAG GAGAACCTGG AGAAGTAATT GGCGAAATTA AAGGAGAAAA GGGGGACCCT
    GGATTCCCAG GTCCAGCTGG ATCTCCTGGT CTGGATGGTG TCCCTGGAAG AGACGGAACC
    CCAGGGATCC CTGGCCCTAA GGGAGAGCCG GGTGGCTTCG TGTTGAAAGG CGATCGTGGT
    TTTCCTGGTG ACATTGGGTT CCCTGGACAT CCTGGAGATC GAGGACTAAT GGGTCCTCCT
    GGTTTTGGTC CCAGTGGTCC ACCTGGAGAG AAGGGAATTC AAGGTGTTTC TGGTCGTCCA
    GGAGCCCCTG GAATACCTGG CCCTAAAGGA TCTCCTGGAC AGACTGTGAT GGAAAAAGGG
    GATTTTGGAC CCCCTGGTCT CCCAGGTATC CCAGGGGATG CCGGAACTCC AGGTGAACCA
    GGTCTACCTG GTCAGCCAGG AATTCCAGGT GTTCCAGGTT CAAAAGGAGA ACCAGGCATT
    CCAGGCATTG GACTTCCTGG GTCTGCAGGC GCAAAAGGAT TTCCTGGTAA TACTGGGCCA
    CCAGGTGCAC CAGGGTTGGC AGGAAGACCT GGTTCAGATG GGTCACCAGG ACAGCCTGGC
    TTGCCAGGAC CAAAGGGTGA CCCTGGGTTT GGTTTACCTG GGCTACCAGG CTCCCCAGGC
    CCTGCTGGAT TGAAAGGATC CCAAGGACCA AAAGGAGAAT CAGGCTTCCC AGGTTCTCCA
    GGCCTTCCTG GACGAGCAGG ATTTGATGGG CCTGTGGGAC CAAAAGGTGA CTCTGGACAA
    AATGGCTTAC CAGGAACCCC TGGACCACCT GGGGTACCTG GTGCTGGTGG TCAAGGCCCT
    CCAGGACAAC CAGGGCTTCC AGGGCCAGCA GGCCCTCCAG GGAACACTGG AATACAGGGA
    GAGAAAGGAG ATCCTGGTGC TCCAGGGTTT GACATGCCAG GCCCACCAGG AGATCGTGGT
    AGCCCTGGAT TTCCAGGAGA AAGAGGGTTT ACAGGTCCAC CAGGGCCACC TGGAGCCCAA
    GGTCGCGATG GATTACCAGG CTTCTCAGGT GCTAAAGGTG AAATGGGTGT AATGGGACCA
    CCAGGATCTG TCGGACCAGC TGGTAATCCT GGAAACCCTG GTTTACCTGG GTCTAAAGGA
    AATGATGGGA ATCCCGGACA ACCTGGCCTG TCTGGTTTAC CAGGGGCTGA AGGTGAAAAG
    GGAGAGAGAG GATTACCAGG ACCCCCTGGA AGAGTTGATT CTGCAAACCT ACAGTTTAAA
    GGAGTTAAAG GAGAACCTGG CTTGCCAGGT TCATCAGGAC TAAGTGGGGC AAAAGGTGCT
    GCTGGACTTC CTGGTAACTC AGGATCCCCA GGGCAGATTG GAATTCCAGG ATCTCCAGGT
    CTTCCAGGGC TCAAGGGAGA TTCTGGCCTT CCTGGTCAGC CGGGTACTTC AGGTCCTCCT
    GGATTAAAAG GGAGCATGGG AGAGATGGGA CTTCCTGGGC CTCCTGGTTC TAAAGGTTCC
    CCTGGACAGC CAGGAAGGCC TGGTTCAGCA GGGTCTCCTG GGTTTTCTGG ACTGAAAGGT
    GAAAAAGGTG AACCAGGAAT TTCAGGAGCT GGTATCCCAG GCTTTCCTGG GCAGAAGGGT
    GAACCAGGGC AAAATGGCTT TCCAGGCAGC CCTGGTAGTA AAGGCAACCC AGGAAACCCA
    GGACTGCCCG GCCTACCAGG GATTTCAGGA CCTAAAGGGG ATCCAGGACT TCCAGGATTC
    CCAGGCAGCC CTGGATCTCC AGGCCTTAAA GGAGTTGATG GGCCCACAGG AAACTCTGGG
    TTACCCGGAT CTCCAGGCTT ACGGGGTGAA TCAGGTCGTC CCGGACCTCA AGGAACTCCT
    GGTGAGAAGG GTCAACCAGG TCAGGATGGA ATTCCTGGGC CTTCTGGTTT GAAGGGAGAA
    CCAGGTTTGC CAGGTTTTGG ATCCCCAGGG CTGCCTGGCC TTCCAGGAAG TCAAGGTCAA
    AAGGGTGATG CAGGACTTTC TGGAAGCCCT GGAAACCCCG GACTTCCAGG GTTCAAAGGA
    GATCAAGGCT TTCCAGGATC TCCTGGGCCA CAAGGACCAA CAGGACAACC TGGCCTACCA
    GGGCCACGTA CAGAAGGACC CAAGGGAGAC CCTGGGCCTG TAGGTCCCCC TGGAAGACCA
    GGCCCTCAAG GTTATCCAGG TCCTCCTGGC TCAGAAGGTC CACGTGGTCC CCCTGGTGAT
    GGAGGCTTTA AAGGAGAAAA AGGAAACCCT GGATTTCCAG GTCAGCCTGG ACTTACAGGA
    TCTAAAGGAG AACATGGAGC ATCTGGTTTC CCAGGTGACC CAGGACGACC AGGTCTTAAT
    GGGATGAAAG GTGATCAAGG TGTTCCTGGT GTTCCAGGAT TCCCAGGTAT GAAAGGACCA
    GGTGGCCCTC CTGGATTGGC TGGCCTTAAA GGTGAATCCG GACTCCCAGG TGCTATAGGT
    TTGCCAGGTC TTCCTGGGCC TAAAGGAGAA TACCGTGAGA TAAAAGGAGC TCCAGGTCTG
    CCAGGTCCTC CAGGTCGCCC AGGAGTAAAT GGACCTCCAG GACCCCCCGG ATCACCAGGA
    CTTCCAGGTT CTTTGGGTCT CCCTGGTGAT ACTGGCAGAG ATGGACTCCC TGGATTTGAG
    GGACAACTTG GACGAAAGGG TGACCGAGGC CTACCAGGCC AACCAGGATC TCGTGGAATT
    AATGGCCCAC CTGGCCCAGA TGGTTTACAA GGCCCCCCTG GCCCCCCTGG TCTAGGATCT
    GTTGCTCATG GATTCCTTAT AACCCGACAC AGTCAGTCAA CAGATGCCCC TGGCTGTCCT
    TTTGGAACCA CCAGGATCTA TGATGGCTAT TCACTACTTT ATGTGCAAGG AAATGAGAGG
    GCACACGGAC AAGATTTGGG TACCGCTGGC AGCTGCCTCC GTCGCTTCAG CACAATGCCT
    TTCATGTTCT GCAACATTAA TAACGTGTGT AACTTTGCTT CAAGAAATGA CTACTCCTAC
    TGGTTGTCCA CGCCTGAGCC TATGCCTATG AGCATGGAAC CTTTAAGAGG GAGGGATATC
    CAACCATTCA TTAGCAGGTG TGCGGTCTGT GAAGCGCCAG CCATGGTGAT TGCAGTTCAC
    AGCCAGACAA TACAAATCCC ACAGTGCCCA CAAGGCTGGT CCTCTCTCTG GATTGGATAC
    TCTTTCATGA TGCATACAAG TGCCGGTGCT GAAGGCTCAG GACAAGCTCT GGCATCGCCT
    GGATCGTGCT TGGAGGAATT CCGATCTGCA CCTTTCATTG AATGTCATGG CCGGGGAACC
    TGCAATTACT ATGCCAATTC ATACAGTTTT TGGTTGGCCA CAGTGGAAAT GTCTGAAATG
    TTTAGCAAAC CTCAGTCAGA AACACTAAAA GCGGGTGATC TAAGGACACG CGTCAGTCGC
    TGCCAAGTTT GTATGAAGAG GACGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa65396
    Clone ID Related Accession (Same CDS sequence) XM_004916867.4
    Accession Version XM_004916867.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5067bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product collagen alpha-5(IV) chain isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004916867.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAGACTGG GAAGTTGGCA GCGGATGCCC TGCCTGGCTG TACTGGTGTT GGTGCTGGGG 
    CACTGGGCAC AGAATGCAGA GTCTGCGGCC TGTCAAGGTT GTACATCGGT ATCGAAATGT
    GACTGCAGTG GTGTTAAAGG AGAAAAGGGT GAGAGAGGCC TTCCAGGGTT AGAAGGACAC
    CTTGGTCTAC CAGGATTTCC TGGACCTGAA GGACCGCCTG GACCTCGTGG TCCCAAGGGT
    GATTCTGGTT TATCAGGGTC TCCAGGACCC AAAGGAATAC GAGGACCTCC TGGACCACCC
    GGATTTCCAG GAAACCCTGG CCAGCCTGGC CTGCCGGGAC ATGATGGTGA ACCAGGGCCT
    GCAGGTATCC CAGGATGCAA TGGCACAAAG GGAGATCGTG GTTTCCCAGG AGGTCCAGGT
    TTTTCGGGTA CACCAGGAAT ACCAGGTCCC CCAGGATTAC CAGGTGTGAA AGGAGATCCA
    GGTGAATCTT TCCCTTTAGC ACCGGGACAA AAAGGTGATC CAGGATTACC AGGTCTTCCA
    GGTTTACCAG GTCGGACTGG TCCTATTGGA GTTCAAGGTC CTGTGGGTCG ACCTGGTCCT
    GCTGGCTTAC CGGGGTCACC AGGAGTACCT GGACCTCCAG GACCCAAGGG TAACATGGGG
    CTGAATTTCC AGGGATCAAA GGGAGAAAAG GGTGAACCTG GTATTCAAGG ACTACCAGGA
    CCACCGGGAC AACTCGAGCA AGTTGAACCT CCACAAACTA CAATTCAGAA GGGAGAGAAG
    GGTATCCCCG GTGACCAAGG ATTTCCAGGA GAGCGTGGGC CAATGGGTCC TCCTGGTCCT
    CCTGGAGGTG CAAAGGGAGA GAAGGGAGAA CCAGGGGAAC CTGGAAAGAT TGGGAAGCCT
    GGAAAAGATG GAAAGATTGG TCTGCCCGGT GGAGAGGGTT TCAGGGGTGA CCCAGGCTTG
    CCGGGAGGTC CAGGCAGAGA TGGGGACAAG GGTCAAAAAG GTGAAAATGG GCTCCCTGGG
    CCACCAGGAG AGGTAATTTC AAGACCAGGG ACAGGCAGAG TTGGACCAAA AGGAAACACA
    GGACCCCCTG GAATAACAGG GCCAAAAGGA GACCGTGGAT TCCCTGGATT ACAAGGTCCC
    CCTGGTTTTC CTGGAACACC AGGTACTTCA AGTGTAGGCC CATCAGGAAA TCCAGGCCTT
    CCTGGTGAAA GAGGGCAAAA AGGCGATGAT GGTCCACCAG GAATCTCTAT TCCAGGGCTT
    CCAGGTTCTG AAGGTCTTGC AGGACCCCCT GGTCCCCCAG GTTTGCCTGG TCGACCTGGA
    ACATCTGCAG TTGGTCCTGC AATGGTATGC CAGCCAGGGC TACCTGGTAC TCAAGGACTT
    CCAGGAGATC CAGGATTTTT AGGAGAAAGA GGACAGAAAG GTGACAAAGG AGACACATGT
    TATAACTGCA TAGGAAATGG TATTTTAGGT CCGAAGGGGG ATCCTGGTTT GCCTGGTTTC
    CCTGGAAGTC CAGGTTCACC TGGAGCTCCA GGGCAAAAAG GAGCAATGGG TATCCCAGGA
    ACAAATGGGC TTCCAGGACC TCCGGGTTTT CCTGGCAATC CTGGTATCTC TGGCCGACCC
    GGTCCCAAAG GAGAACCTGG AGAAGTAATT GGCGAAATTA AAGGAGAAAA GGGGGACCCT
    GGATTCCCAG GTCCAGCTGG ATCTCCTGGT CTGGATGGTG TCCCTGGAAG AGACGGAACC
    CCAGGGATCC CTGGCCCTAA GGGAGAGCCG GGTGGCTTCG TGTTGAAAGG CGATCGTGGT
    TTTCCTGGTG ACATTGGGTT CCCTGGACAT CCTGGAGATC GAGGACTAAT GGGTCCTCCT
    GGTTTTGGTC CCAGTGGTCC ACCTGGAGAG AAGGGAATTC AAGGTGTTTC TGGTCGTCCA
    GGAGCCCCTG GAATACCTGG CCCTAAAGGA TCTCCTGGAC AGACTGTGAT GGAAAAAGGG
    GATTTTGGAC CCCCTGGTCT CCCAGGTATC CCAGGGGATG CCGGAACTCC AGGTGAACCA
    GGTCTACCTG GTCAGCCAGG AATTCCAGGT GTTCCAGGTT CAAAAGGAGA ACCAGGCATT
    CCAGGCATTG GACTTCCTGG GTCTGCAGGC GCAAAAGGAT TTCCTGGTAA TACTGGGCCA
    CCAGGTGCAC CAGGGTTGGC AGGAAGACCT GGTTCAGATG GGTCACCAGG ACAGCCTGGC
    TTGCCAGGAC CAAAGGGTGA CCCTGGGTTT GGTTTACCTG GGCTACCAGG CTCCCCAGGC
    CCTGCTGGAT TGAAAGGATC CCAAGGACCA AAAGGAGAAT CAGGCTTCCC AGGTTCTCCA
    GGCCTTCCTG GACGAGCAGG ATTTGATGGG CCTGTGGGAC CAAAAGGTGA CTCTGGACAA
    AATGGCTTAC CAGGAACCCC TGGACCACCT GGGGTACCTG GTGCTGGTGG TCAAGGCCCT
    CCAGGACAAC CAGGGCTTCC AGGGCCAGCA GGCCCTCCAG GTCCCACAGG GAACACTGGA
    ATACAGGGAG AGAAAGGAGA TCCTGGTGCT CCAGGGTTTG ACATGCCAGG CCCACCAGGA
    GATCGTGGTA GCCCTGGATT TCCAGGAGAA AGAGGGTTTA CAGGTCCACC AGGGCCACCT
    GGAGCCCAAG GTCGCGATGG ATTACCAGGC TTCTCAGGTG CTAAAGGTGA AATGGGTGTA
    ATGGGACCAC CAGGATCTGT CGGACCAGCT GGTAATCCTG GAAACCCTGG TTTACCTGGG
    TCTAAAGGAA ATGATGGGAA TCCCGGACAA CCTGGCCTGT CTGGTTTACC AGGGGCTGAA
    GGTGAAAAGG GAGAGAGAGG ATTACCAGGA CCCCCTGGAA GAGTTGATTC TGCAAACCTA
    CAGTTTAAAG GAGTTAAAGG AGAACCTGGC TTGCCAGGTT CATCAGGACT AAGTGGGGCA
    AAAGGTGCTG CTGGACTTCC TGGTAACTCA GGATCCCCAG GGCAGATTGG AATTCCAGGA
    TCTCCAGGTC TTCCAGGGCT CAAGGGAGAT TCTGGCCTTC CTGGTCAGCC GGGTACTTCA
    GGTCCTCCTG GATTAAAAGG GAGCATGGGA GAGATGGGAC TTCCTGGGCC TCCTGGTTCT
    AAAGGTTCCC CTGGACAGCC AGGAAGGCCT GGTTCAGCAG GGTCTCCTGG GTTTTCTGGA
    CTGAAAGGTG AAAAAGGTGA ACCAGGAATT TCAGGAGCTG GTATCCCAGG CTTTCCTGGG
    CAGAAGGGTG AACCAGGGCA AAATGGCTTT CCAGGCAGCC CTGGTAGTAA AGGCAACCCA
    GGAAACCCAG GACTGCCCGG CCTACCAGGG ATTTCAGGAC CTAAAGGGGA TCCAGGACTT
    CCAGGATTCC CAGGCAGCCC TGGATCTCCA GGCCTTAAAG GAGTTGATGG GCCCACAGGA
    AACTCTGGGT TACCCGGATC TCCAGGCTTA CGGGGTGAAT CAGGTCGTCC CGGACCTCAA
    GGAACTCCTG GTGAGAAGGG TCAACCAGGT CAGGATGGAA TTCCTGGGCC TTCTGGTTTG
    AAGGGAGAAC CAGGTTTGCC AGGTTTTGGA TCCCCAGGGC TGCCTGGCCT TCCAGGAAGT
    CAAGGTCAAA AGGGTGATGC AGGACTTTCT GGAAGCCCTG GAAACCCCGG ACTTCCAGGG
    TTCAAAGGAG ATCAAGGCTT TCCAGGATCT CCTGGGCCAC AAGGACCAAC AGGACAACCT
    GGCCTACCAG GGCCACGTAC AGAAGGACCC AAGGGAGACC CTGGGCCTGT AGGTCCCCCT
    GGAAGACCAG GTTATCCAGG TCCTCCTGGC TCAGAAGGTC CACGTGGTCC CCCTGGTGAT
    GGAGGCTTTA AAGGAGAAAA AGGAAACCCT GGATTTCCAG GTCAGCCTGG ACTTACAGGA
    TCTAAAGGAG AACATGGAGC ATCTGGTTTC CCAGGTGACC CAGGACGACC AGGTCTTAAT
    GGGATGAAAG GTGATCAAGG TGTTCCTGGT GTTCCAGGAT TCCCAGGTAT GAAAGGACCA
    GGTGGCCCTC CTGGATTGGC TGGCCTTAAA GGTGAATCCG GACTCCCAGG TGCTATAGGT
    TTGCCAGGTC TTCCTGGGCC TAAAGGAGAA TACCGTGAGA TAAAAGGAGC TCCAGGTCTG
    CCAGGTCCTC CAGGTCGCCC AGGAGTAAAT GGACCTCCAG GACCCCCCGG ATCACCAGGA
    CTTCCAGGTT CTTTGGGTCT CCCTGGTGAT ACTGGCAGAG ATGGACTCCC TGGATTTGAG
    GGACAACTTG GACGAAAGGG TGACCGAGGC CTACCAGGCC AACCAGGATC TCGTGGAATT
    AATGGCCCAC CTGGCCCAGA TGGTTTACAA GGCCCCCCTG GCCCCCCTGG TCTAGGATCT
    GTTGCTCATG GATTCCTTAT AACCCGACAC AGTCAGTCAA CAGATGCCCC TGGCTGTCCT
    TTTGGAACCA CCAGGATCTA TGATGGCTAT TCACTACTTT ATGTGCAAGG AAATGAGAGG
    GCACACGGAC AAGATTTGGG TACCGCTGGC AGCTGCCTCC GTCGCTTCAG CACAATGCCT
    TTCATGTTCT GCAACATTAA TAACGTGTGT AACTTTGCTT CAAGAAATGA CTACTCCTAC
    TGGTTGTCCA CGCCTGAGCC TATGCCTATG AGCATGGAAC CTTTAAGAGG GAGGGATATC
    CAACCATTCA TTAGCAGGTG TGCGGTCTGT GAAGCGCCAG CCATGGTGAT TGCAGTTCAC
    AGCCAGACAA TACAAATCCC ACAGTGCCCA CAAGGCTGGT CCTCTCTCTG GATTGGATAC
    TCTTTCATGA TGCATACAAG TGCCGGTGCT GAAGGCTCAG GACAAGCTCT GGCATCGCCT
    GGATCGTGCT TGGAGGAATT CCGATCTGCA CCTTTCATTG AATGTCATGG CCGGGGAACC
    TGCAATTACT ATGCCAATTC ATACAGTTTT TGGTTGGCCA CAGTGGAAAT GTCTGAAATG
    TTTAGCAAAC CTCAGTCAGA AACACTAAAA GCGGGTGATC TAAGGACACG CGTCAGTCGC
    TGCCAAGTTT GTATGAAGAG GACGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004916924.2
    CDS281..5347
    Translation

    Target ORF information:

    RefSeq Version XM_004916867.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis collagen, type IV, alpha 5 (col4a5), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004916867.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAGACTGG GAAGTTGGCA GCGGATGCCC TGCCTGGCTG TACTGGTGTT GGTGCTGGGG 
    CACTGGGCAC AGAATGCAGA GTCTGCGGCC TGTCAAGGTT GTACATCGGT ATCGAAATGT
    GACTGCAGTG GTGTTAAAGG AGAAAAGGGT GAGAGAGGCC TTCCAGGGTT AGAAGGACAC
    CTTGGTCTAC CAGGATTTCC TGGACCTGAA GGACCGCCTG GACCTCGTGG TCCCAAGGGT
    GATTCTGGTT TATCAGGGTC TCCAGGACCC AAAGGAATAC GAGGACCTCC TGGACCACCC
    GGATTTCCAG GAAACCCTGG CCAGCCTGGC CTGCCGGGAC ATGATGGTGA ACCAGGGCCT
    GCAGGTATCC CAGGATGCAA TGGCACAAAG GGAGATCGTG GTTTCCCAGG AGGTCCAGGT
    TTTTCGGGTA CACCAGGAAT ACCAGGTCCC CCAGGATTAC CAGGTGTGAA AGGAGATCCA
    GGTGAATCTT TCCCTTTAGC ACCGGGACAA AAAGGTGATC CAGGATTACC AGGTCTTCCA
    GGTTTACCAG GTCGGACTGG TCCTATTGGA GTTCAAGGTC CTGTGGGTCG ACCTGGTCCT
    GCTGGCTTAC CGGGGTCACC AGGAGTACCT GGACCTCCAG GACCCAAGGG TAACATGGGG
    CTGAATTTCC AGGGATCAAA GGGAGAAAAG GGTGAACCTG GTATTCAAGG ACTACCAGGA
    CCACCGGGAC AACTCGAGCA AGTTGAACCT CCACAAACTA CAATTCAGAA GGGAGAGAAG
    GGTATCCCCG GTGACCAAGG ATTTCCAGGA GAGCGTGGGC CAATGGGTCC TCCTGGTCCT
    CCTGGAGGTG CAAAGGGAGA GAAGGGAGAA CCAGGGGAAC CTGGAAAGAT TGGGAAGCCT
    GGAAAAGATG GAAAGATTGG TCTGCCCGGT GGAGAGGGTT TCAGGGGTGA CCCAGGCTTG
    CCGGGAGGTC CAGGCAGAGA TGGGGACAAG GGTCAAAAAG GTGAAAATGG GCTCCCTGGG
    CCACCAGGAG AGGTAATTTC AAGACCAGGG ACAGGCAGAG TTGGACCAAA AGGAAACACA
    GGACCCCCTG GAATAACAGG GCCAAAAGGA GACCGTGGAT TCCCTGGATT ACAAGGTCCC
    CCTGGTTTTC CTGGAACACC AGGTACTTCA AGTGTAGGCC CATCAGGAAA TCCAGGCCTT
    CCTGGTGAAA GAGGGCAAAA AGGCGATGAT GGTCCACCAG GAATCTCTAT TCCAGGGCTT
    CCAGGTTCTG AAGGTCTTGC AGGACCCCCT GGTCCCCCAG GTTTGCCTGG TCGACCTGGA
    ACATCTGCAG TTGGTCCTGC AATGGTATGC CAGCCAGGGC TACCTGGTAC TCAAGGACTT
    CCAGGAGATC CAGGATTTTT AGGAGAAAGA GGACAGAAAG GTGACAAAGG AGACACATGT
    TATAACTGCA TAGGAAATGG TATTTTAGGT CCGAAGGGGG ATCCTGGTTT GCCTGGTTTC
    CCTGGAAGTC CAGGTTCACC TGGAGCTCCA GGGCAAAAAG GAGCAATGGG TATCCCAGGA
    ACAAATGGGC TTCCAGGACC TCCGGGTTTT CCTGGCAATC CTGGTATCTC TGGCCGACCC
    GGTCCCAAAG GAGAACCTGG AGAAGTAATT GGCGAAATTA AAGGAGAAAA GGGGGACCCT
    GGATTCCCAG GTCCAGCTGG ATCTCCTGGT CTGGATGGTG TCCCTGGAAG AGACGGAACC
    CCAGGGATCC CTGGCCCTAA GGGAGAGCCG GGTGGCTTCG TGTTGAAAGG CGATCGTGGT
    TTTCCTGGTG ACATTGGGTT CCCTGGACAT CCTGGAGATC GAGGACTAAT GGGTCCTCCT
    GGTTTTGGTC CCAGTGGTCC ACCTGGAGAG AAGGGAATTC AAGGTGTTTC TGGTCGTCCA
    GGAGCCCCTG GAATACCTGG CCCTAAAGGA TCTCCTGGAC AGACTGTGAT GGAAAAAGGG
    GATTTTGGAC CCCCTGGTCT CCCAGGTATC CCAGGGGATG CCGGAACTCC AGGTGAACCA
    GGTCTACCTG GTCAGCCAGG AATTCCAGGT GTTCCAGGTT CAAAAGGAGA ACCAGGCATT
    CCAGGCATTG GACTTCCTGG GTCTGCAGGC GCAAAAGGAT TTCCTGGTAA TACTGGGCCA
    CCAGGTGCAC CAGGGTTGGC AGGAAGACCT GGTTCAGATG GGTCACCAGG ACAGCCTGGC
    TTGCCAGGAC CAAAGGGTGA CCCTGGGTTT GGTTTACCTG GGCTACCAGG CTCCCCAGGC
    CCTGCTGGAT TGAAAGGATC CCAAGGACCA AAAGGAGAAT CAGGCTTCCC AGGTTCTCCA
    GGCCTTCCTG GACGAGCAGG ATTTGATGGG CCTGTGGGAC CAAAAGGTGA CTCTGGACAA
    AATGGCTTAC CAGGAACCCC TGGACCACCT GGGGTACCTG GTGCTGGTGG TCAAGGCCCT
    CCAGGACAAC CAGGGCTTCC AGGGCCAGCA GGCCCTCCAG GTCCCACAGG GAACACTGGA
    ATACAGGGAG AGAAAGGAGA TCCTGGTGCT CCAGGGTTTG ACATGCCAGG CCCACCAGGA
    GATCGTGGTA GCCCTGGATT TCCAGGAGAA AGAGGGTTTA CAGGTCCACC AGGGCCACCT
    GGAGCCCAAG GTCGCGATGG ATTACCAGGC TTCTCAGGTG CTAAAGGTGA AATGGGTGTA
    ATGGGACCAC CAGGATCTGT CGGACCAGCT GGTAATCCTG GAAACCCTGG TTTACCTGGG
    TCTAAAGGAA ATGATGGGAA TCCCGGACAA CCTGGCCTGT CTGGTTTACC AGGGGCTGAA
    GGTGAAAAGG GAGAGAGAGG ATTACCAGGA CCCCCTGGAA GAGTTGATTC TGCAAACCTA
    CAGTTTAAAG GAGTTAAAGG AGAACCTGGC TTGCCAGGTT CATCAGGACT AAGTGGGGCA
    AAAGGTGCTG CTGGACTTCC TGGTAACTCA GGATCCCCAG GGCAGATTGG AATTCCAGGA
    TCTCCAGGTC TTCCAGGGCT CAAGGGAGAT TCTGGCCTTC CTGGTCAGCC GGGTACTTCA
    GGTCCTCCTG GATTAAAAGG GAGCATGGGA GAGATGGGAC TTCCTGGGCC TCCTGGTTCT
    AAAGGTTCCC CTGGACAGCC AGGAAGGCCT GGTTCAGCAG GGTCTCCTGG GTTTTCTGGA
    CTGAAAGGTG AAAAAGGTGA ACCAGGAATT TCAGGAGCTG GTATCCCAGG CTTTCCTGGG
    CAGAAGGGTG AACCAGGGCA AAATGGCTTT CCAGGCAGCC CTGGTAGTAA AGGCAACCCA
    GGAAACCCAG GACTGCCCGG CCTACCAGGG ATTTCAGGAC CTAAAGGGGA TCCAGGACTT
    CCAGGATTCC CAGGCAGCCC TGGATCTCCA GGCCTTAAAG GAGTTGATGG GCCCACAGGA
    AACTCTGGGT TACCCGGATC TCCAGGCTTA CGGGGTGAAT CAGGTCGTCC CGGACCTCAA
    GGAACTCCTG GTGAGAAGGG TCAACCAGGT CAGGATGGAA TTCCTGGGCC TTCTGGTTTG
    AAGGGAGAAC CAGGTTTGCC AGGTTTTGGA TCCCCAGGGC TGCCTGGCCT TCCAGGAAGT
    CAAGGTCAAA AGGGTGATGC AGGACTTTCT GGAAGCCCTG GAAACCCCGG ACTTCCAGGG
    TTCAAAGGAG ATCAAGGCTT TCCAGGATCT CCTGGGCCAC AAGGACCAAC AGGACAACCT
    GGCCTACCAG GGCCACGTAC AGAAGGACCC AAGGGAGACC CTGGGCCTGT AGGTCCCCCT
    GGAAGACCAG GTTATCCAGG TCCTCCTGGC TCAGAAGGTC CACGTGGTCC CCCTGGTGAT
    GGAGGCTTTA AAGGAGAAAA AGGAAACCCT GGATTTCCAG GTCAGCCTGG ACTTACAGGA
    TCTAAAGGAG AACATGGAGC ATCTGGTTTC CCAGGTGACC CAGGACGACC AGGTCTTAAT
    GGGATGAAAG GTGATCAAGG TGTTCCTGGT GTTCCAGGAT TCCCAGGTAT GAAAGGACCA
    GGTGGCCCTC CTGGATTGGC TGGCCTTAAA GGTGAATCCG GACTCCCAGG TGCTATAGGT
    TTGCCAGGTC TTCCTGGGCC TAAAGGAGAA TACCGTGAGA TAAAAGGAGC TCCAGGTCTG
    CCAGGTCCTC CAGGTCGCCC AGGAGTAAAT GGACCTCCAG GACCCCCCGG ATCACCAGGA
    CTTCCAGGTT CTTTGGGTCT CCCTGGTGAT ACTGGCAGAG ATGGACTCCC TGGATTTGAG
    GGACAACTTG GACGAAAGGG TGACCGAGGC CTACCAGGCC AACCAGGATC TCGTGGAATT
    AATGGCCCAC CTGGCCCAGA TGGTTTACAA GGCCCCCCTG GCCCCCCTGG TCTAGGATCT
    GTTGCTCATG GATTCCTTAT AACCCGACAC AGTCAGTCAA CAGATGCCCC TGGCTGTCCT
    TTTGGAACCA CCAGGATCTA TGATGGCTAT TCACTACTTT ATGTGCAAGG AAATGAGAGG
    GCACACGGAC AAGATTTGGG TACCGCTGGC AGCTGCCTCC GTCGCTTCAG CACAATGCCT
    TTCATGTTCT GCAACATTAA TAACGTGTGT AACTTTGCTT CAAGAAATGA CTACTCCTAC
    TGGTTGTCCA CGCCTGAGCC TATGCCTATG AGCATGGAAC CTTTAAGAGG GAGGGATATC
    CAACCATTCA TTAGCAGGTG TGCGGTCTGT GAAGCGCCAG CCATGGTGAT TGCAGTTCAC
    AGCCAGACAA TACAAATCCC ACAGTGCCCA CAAGGCTGGT CCTCTCTCTG GATTGGATAC
    TCTTTCATGA TGCATACAAG TGCCGGTGCT GAAGGCTCAG GACAAGCTCT GGCATCGCCT
    GGATCGTGCT TGGAGGAATT CCGATCTGCA CCTTTCATTG AATGTCATGG CCGGGGAACC
    TGCAATTACT ATGCCAATTC ATACAGTTTT TGGTTGGCCA CAGTGGAAAT GTCTGAAATG
    TTTAGCAAAC CTCAGTCAGA AACACTAAAA GCGGGTGATC TAAGGACACG CGTCAGTCGC
    TGCCAAGTTT GTATGAAGAG GACGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa65397
    Clone ID Related Accession (Same CDS sequence) XM_004916868.4
    Accession Version XM_004916868.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5058bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product collagen alpha-5(IV) chain isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004916868.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAGACTGG GAAGTTGGCA GCGGATGCCC TGCCTGGCTG TACTGGTGTT GGTGCTGGGG 
    CACTGGGCAC AGAATGCAGA GTCTGCGGCC TGTCAAGGTT GTACATCGGT ATCGAAATGT
    GACTGCAGTG GTGTTAAAGG AGAAAAGGGT GAGAGAGGCC TTCCAGGGTT AGAAGGACAC
    CTTGGTCTAC CAGGATTTCC TGGACCTGAA GGACCGCCTG GACCTCGTGG TCCCAAGGGT
    GATTCTGGTT TATCAGGGTC TCCAGGACCC AAAGGAATAC GAGGACCTCC TGGACCACCC
    GGATTTCCAG GAAACCCTGG CCAGCCTGGC CTGCCGGGAC ATGATGGTGA ACCAGGGCCT
    GCAGGTATCC CAGGATGCAA TGGCACAAAG GGAGATCGTG GTTTCCCAGG AGGTCCAGGT
    TTTTCGGGTA CACCAGGAAT ACCAGGTCCC CCAGGATTAC CAGGTGTGAA AGGAGATCCA
    GGTGAATCTT TCCCTTTAGC ACCGGGACAA AAAGGTGATC CAGGATTACC AGGTCTTCCA
    GGTTTACCAG GTCGGACTGG TCCTATTGGA GTTCAAGGTC CTGTGGGTCG ACCTGGTCCT
    GCTGGCTTAC CGGGGTCACC AGGAGTACCT GGACCTCCAG GACCCAAGGG TAACATGGGG
    CTGAATTTCC AGGGATCAAA GGGAGAAAAG GGTGAACCTG GTATTCAAGG ACTACCAGGA
    CCACCGGGAC AACTCGAGCA AGTTGAACCT CCACAAACTA CAATTCAGAA GGGAGAGAAG
    GGTATCCCCG GTGACCAAGG ATTTCCAGGA GAGCGTGGGC CAATGGGTCC TCCTGGTCCT
    CCTGGAGGTG CAAAGGGAGA GAAGGGAGAA CCAGGGGAAC CTGGAAAGAT TGGGAAGCCT
    GGAAAAGATG GAAAGATTGG TCTGCCCGGT GGAGAGGGTT TCAGGGGTGA CCCAGGCTTG
    CCGGGAGGTC CAGGCAGAGA TGGGGACAAG GGTCAAAAAG GTGAAAATGG GCTCCCTGGG
    CCACCAGGAG AGGTAATTTC AAGACCAGGG ACAGGCAGAG TTGGACCAAA AGGAAACACA
    GGACCCCCTG GAATAACAGG GCCAAAAGGA GACCGTGGAT TCCCTGGATT ACAAGGTCCC
    CCTGGTTTTC CTGGAACACC AGGTACTTCA AGTGTAGGCC CATCAGGAAA TCCAGGCCTT
    CCTGGTGAAA GAGGGCAAAA AGGCGATGAT GGTCCACCAG GAATCTCTAT TCCAGGGCTT
    CCAGGTTCTG AAGGTCTTGC AGGACCCCCT GGTCCCCCAG GTTTGCCTGG TCGACCTGGA
    ACATCTGCAG TTGGTCCTGC AATGGTATGC CAGCCAGGGC TACCTGGTAC TCAAGGACTT
    CCAGGAGATC CAGGATTTTT AGGAGAAAGA GGACAGAAAG GTGACAAAGG AGACACATGT
    TATAACTGCA TAGGAAATGG TATTTTAGGT CCGAAGGGGG ATCCTGGTTT GCCTGGTTTC
    CCTGGAAGTC CAGGTTCACC TGGAGCTCCA GGGCAAAAAG GAGCAATGGG TATCCCAGGA
    ACAAATGGGC TTCCAGGACC TCCGGGTTTT CCTGGCAATC CTGGTATCTC TGGCCGACCC
    GGTCCCAAAG GAGAACCTGG AGAAGTAATT GGCGAAATTA AAGGAGAAAA GGGGGACCCT
    GGATTCCCAG GTCCAGCTGG ATCTCCTGGT CTGGATGGTG TCCCTGGAAG AGACGGAACC
    CCAGGGATCC CTGGCCCTAA GGGAGAGCCG GGTGGCTTCG TGTTGAAAGG CGATCGTGGT
    TTTCCTGGTG ACATTGGGTT CCCTGGACAT CCTGGAGATC GAGGACTAAT GGGTCCTCCT
    GGTTTTGGTC CCAGTGGTCC ACCTGGAGAG AAGGGAATTC AAGGTGTTTC TGGTCGTCCA
    GGAGCCCCTG GAATACCTGG CCCTAAAGGA TCTCCTGGAC AGACTGTGAT GGAAAAAGGG
    GATTTTGGAC CCCCTGGTCT CCCAGGTATC CCAGGGGATG CCGGAACTCC AGGTGAACCA
    GGTCTACCTG GTCAGCCAGG AATTCCAGGT GTTCCAGGTT CAAAAGGAGA ACCAGGCATT
    CCAGGCATTG GACTTCCTGG GTCTGCAGGC GCAAAAGGAT TTCCTGGTAA TACTGGGCCA
    CCAGGTGCAC CAGGGTTGGC AGGAAGACCT GGTTCAGATG GGTCACCAGG ACAGCCTGGC
    TTGCCAGGAC CAAAGGGTGA CCCTGGGTTT GGTTTACCTG GGCTACCAGG CTCCCCAGGC
    CCTGCTGGAT TGAAAGGATC CCAAGGACCA AAAGGAGAAT CAGGCTTCCC AGGTTCTCCA
    GGCCTTCCTG GACGAGCAGG ATTTGATGGG CCTGTGGGAC CAAAAGGTGA CTCTGGACAA
    AATGGCTTAC CAGGAACCCC TGGACCACCT GGGGTACCTG GTGCTGGTGG TCAAGGCCCT
    CCAGGACAAC CAGGGCTTCC AGGGCCAGCA GGCCCTCCAG GTCCCACAGG GAACACTGGA
    ATACAGGGAG AGAAAGGAGA TCCTGGTGCT CCAGGGTTTG ACATGCCAGG CCCACCAGGA
    GATCGTGGTA GCCCTGGATT TCCAGGAGAA AGAGGGTTTA CAGGTCCACC AGGGCCACCT
    GGAGCCCAAG GTCGCGATGG ATTACCAGGC TTCTCAGGTG CTAAAGGTGA AATGGGTGTA
    ATGGGACCAC CAGGATCTGT CGGACCAGCT GGTAATCCTG GAAACCCTGG TTTACCTGGG
    TCTAAAGGAA ATGATGGGAA TCCCGGACAA CCTGGCCTGT CTGGTTTACC AGGGGCTGAA
    GGTGAAAAGG GAGAGAGAGG ATTACCAGGA CCCCCTGGAA GAGTTGATTC TGCAAACCTA
    CAGTTTAAAG GAGTTAAAGG AGAACCTGGC TTGCCAGGTT CATCAGGACT AAGTGGGGCA
    AAAGGTGCTG CTGGACTTCC TGGTAACTCA GGATCCCCAG GGCAGATTGG AATTCCAGGA
    TCTCCAGGTC TTCCAGGGCT CAAGGGAGAT TCTGGCCTTC CTGGTCAGCC GGGTACTTCA
    GGTCCTCCTG GATTAAAAGG GAGCATGGGA GAGATGGGAC TTCCTGGGCC TCCTGGTTCT
    AAAGGTTCCC CTGGACAGCC AGGAAGGCCT GGTTCAGCAG GGTCTCCTGG GTTTTCTGGA
    CTGAAAGGTG AAAAAGGTGA ACCAGGAATT TCAGGAGCTG GTATCCCAGG CTTTCCTGGG
    CAGAAGGGTG AACCAGGGCA AAATGGCTTT CCAGGCAGCC CTGGTAGTAA AGGCAACCCA
    GGAAACCCAG GACTGCCCGG CCTACCAGGG ATTTCAGGAC CTAAAGGGGA TCCAGGACTT
    CCAGGATTCC CAGGCAGCCC TGGATCTCCA GGCCTTAAAG GAGTTGATGG GCCCACAGGA
    AACTCTGGGT TACCCGGATC TCCAGGCTTA CGGGGTGAAT CAGGTCGTCC CGGACCTCAA
    GGAACTCCTG GTGAGAAGGG TCAACCAGGT CAGGATGGAA TTCCTGGGCC TTCTGGTTTG
    AAGGGAGAAC CAGGTTTGCC AGGTTTTGGA TCCCCAGGGC TGCCTGGCCT TCCAGGAAGT
    CAAGGTCAAA AGGGTGATGC AGGACTTTCT GGAAGCCCTG GAAACCCCGG ACTTCCAGGG
    TTCAAAGGAG ATCAAGGCTT TCCAGGATCT CCTGGGCCAC AAGGACCAAC AGGACAACCT
    GGCCTACCAG GGCCACGTAC AGAAGGACCC AAGGGAGACC CTGGGCCTGT AGGTCCCCCT
    GGAAGACCAG GTCCTCCTGG CTCAGAAGGT CCACGTGGTC CCCCTGGTGA TGGAGGCTTT
    AAAGGAGAAA AAGGAAACCC TGGATTTCCA GGTCAGCCTG GACTTACAGG ATCTAAAGGA
    GAACATGGAG CATCTGGTTT CCCAGGTGAC CCAGGACGAC CAGGTCTTAA TGGGATGAAA
    GGTGATCAAG GTGTTCCTGG TGTTCCAGGA TTCCCAGGTA TGAAAGGACC AGGTGGCCCT
    CCTGGATTGG CTGGCCTTAA AGGTGAATCC GGACTCCCAG GTGCTATAGG TTTGCCAGGT
    CTTCCTGGGC CTAAAGGAGA ATACCGTGAG ATAAAAGGAG CTCCAGGTCT GCCAGGTCCT
    CCAGGTCGCC CAGGAGTAAA TGGACCTCCA GGACCCCCCG GATCACCAGG ACTTCCAGGT
    TCTTTGGGTC TCCCTGGTGA TACTGGCAGA GATGGACTCC CTGGATTTGA GGGACAACTT
    GGACGAAAGG GTGACCGAGG CCTACCAGGC CAACCAGGAT CTCGTGGAAT TAATGGCCCA
    CCTGGCCCAG ATGGTTTACA AGGCCCCCCT GGCCCCCCTG GTCTAGGATC TGTTGCTCAT
    GGATTCCTTA TAACCCGACA CAGTCAGTCA ACAGATGCCC CTGGCTGTCC TTTTGGAACC
    ACCAGGATCT ATGATGGCTA TTCACTACTT TATGTGCAAG GAAATGAGAG GGCACACGGA
    CAAGATTTGG GTACCGCTGG CAGCTGCCTC CGTCGCTTCA GCACAATGCC TTTCATGTTC
    TGCAACATTA ATAACGTGTG TAACTTTGCT TCAAGAAATG ACTACTCCTA CTGGTTGTCC
    ACGCCTGAGC CTATGCCTAT GAGCATGGAA CCTTTAAGAG GGAGGGATAT CCAACCATTC
    ATTAGCAGGT GTGCGGTCTG TGAAGCGCCA GCCATGGTGA TTGCAGTTCA CAGCCAGACA
    ATACAAATCC CACAGTGCCC ACAAGGCTGG TCCTCTCTCT GGATTGGATA CTCTTTCATG
    ATGCATACAA GTGCCGGTGC TGAAGGCTCA GGACAAGCTC TGGCATCGCC TGGATCGTGC
    TTGGAGGAAT TCCGATCTGC ACCTTTCATT GAATGTCATG GCCGGGGAAC CTGCAATTAC
    TATGCCAATT CATACAGTTT TTGGTTGGCC ACAGTGGAAA TGTCTGAAAT GTTTAGCAAA
    CCTCAGTCAG AAACACTAAA AGCGGGTGAT CTAAGGACAC GCGTCAGTCG CTGCCAAGTT
    TGTATGAAGA GGACGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004916925.2
    CDS280..5337
    Translation

    Target ORF information:

    RefSeq Version XM_004916868.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis collagen, type IV, alpha 5 (col4a5), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004916868.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAGACTGG GAAGTTGGCA GCGGATGCCC TGCCTGGCTG TACTGGTGTT GGTGCTGGGG 
    CACTGGGCAC AGAATGCAGA GTCTGCGGCC TGTCAAGGTT GTACATCGGT ATCGAAATGT
    GACTGCAGTG GTGTTAAAGG AGAAAAGGGT GAGAGAGGCC TTCCAGGGTT AGAAGGACAC
    CTTGGTCTAC CAGGATTTCC TGGACCTGAA GGACCGCCTG GACCTCGTGG TCCCAAGGGT
    GATTCTGGTT TATCAGGGTC TCCAGGACCC AAAGGAATAC GAGGACCTCC TGGACCACCC
    GGATTTCCAG GAAACCCTGG CCAGCCTGGC CTGCCGGGAC ATGATGGTGA ACCAGGGCCT
    GCAGGTATCC CAGGATGCAA TGGCACAAAG GGAGATCGTG GTTTCCCAGG AGGTCCAGGT
    TTTTCGGGTA CACCAGGAAT ACCAGGTCCC CCAGGATTAC CAGGTGTGAA AGGAGATCCA
    GGTGAATCTT TCCCTTTAGC ACCGGGACAA AAAGGTGATC CAGGATTACC AGGTCTTCCA
    GGTTTACCAG GTCGGACTGG TCCTATTGGA GTTCAAGGTC CTGTGGGTCG ACCTGGTCCT
    GCTGGCTTAC CGGGGTCACC AGGAGTACCT GGACCTCCAG GACCCAAGGG TAACATGGGG
    CTGAATTTCC AGGGATCAAA GGGAGAAAAG GGTGAACCTG GTATTCAAGG ACTACCAGGA
    CCACCGGGAC AACTCGAGCA AGTTGAACCT CCACAAACTA CAATTCAGAA GGGAGAGAAG
    GGTATCCCCG GTGACCAAGG ATTTCCAGGA GAGCGTGGGC CAATGGGTCC TCCTGGTCCT
    CCTGGAGGTG CAAAGGGAGA GAAGGGAGAA CCAGGGGAAC CTGGAAAGAT TGGGAAGCCT
    GGAAAAGATG GAAAGATTGG TCTGCCCGGT GGAGAGGGTT TCAGGGGTGA CCCAGGCTTG
    CCGGGAGGTC CAGGCAGAGA TGGGGACAAG GGTCAAAAAG GTGAAAATGG GCTCCCTGGG
    CCACCAGGAG AGGTAATTTC AAGACCAGGG ACAGGCAGAG TTGGACCAAA AGGAAACACA
    GGACCCCCTG GAATAACAGG GCCAAAAGGA GACCGTGGAT TCCCTGGATT ACAAGGTCCC
    CCTGGTTTTC CTGGAACACC AGGTACTTCA AGTGTAGGCC CATCAGGAAA TCCAGGCCTT
    CCTGGTGAAA GAGGGCAAAA AGGCGATGAT GGTCCACCAG GAATCTCTAT TCCAGGGCTT
    CCAGGTTCTG AAGGTCTTGC AGGACCCCCT GGTCCCCCAG GTTTGCCTGG TCGACCTGGA
    ACATCTGCAG TTGGTCCTGC AATGGTATGC CAGCCAGGGC TACCTGGTAC TCAAGGACTT
    CCAGGAGATC CAGGATTTTT AGGAGAAAGA GGACAGAAAG GTGACAAAGG AGACACATGT
    TATAACTGCA TAGGAAATGG TATTTTAGGT CCGAAGGGGG ATCCTGGTTT GCCTGGTTTC
    CCTGGAAGTC CAGGTTCACC TGGAGCTCCA GGGCAAAAAG GAGCAATGGG TATCCCAGGA
    ACAAATGGGC TTCCAGGACC TCCGGGTTTT CCTGGCAATC CTGGTATCTC TGGCCGACCC
    GGTCCCAAAG GAGAACCTGG AGAAGTAATT GGCGAAATTA AAGGAGAAAA GGGGGACCCT
    GGATTCCCAG GTCCAGCTGG ATCTCCTGGT CTGGATGGTG TCCCTGGAAG AGACGGAACC
    CCAGGGATCC CTGGCCCTAA GGGAGAGCCG GGTGGCTTCG TGTTGAAAGG CGATCGTGGT
    TTTCCTGGTG ACATTGGGTT CCCTGGACAT CCTGGAGATC GAGGACTAAT GGGTCCTCCT
    GGTTTTGGTC CCAGTGGTCC ACCTGGAGAG AAGGGAATTC AAGGTGTTTC TGGTCGTCCA
    GGAGCCCCTG GAATACCTGG CCCTAAAGGA TCTCCTGGAC AGACTGTGAT GGAAAAAGGG
    GATTTTGGAC CCCCTGGTCT CCCAGGTATC CCAGGGGATG CCGGAACTCC AGGTGAACCA
    GGTCTACCTG GTCAGCCAGG AATTCCAGGT GTTCCAGGTT CAAAAGGAGA ACCAGGCATT
    CCAGGCATTG GACTTCCTGG GTCTGCAGGC GCAAAAGGAT TTCCTGGTAA TACTGGGCCA
    CCAGGTGCAC CAGGGTTGGC AGGAAGACCT GGTTCAGATG GGTCACCAGG ACAGCCTGGC
    TTGCCAGGAC CAAAGGGTGA CCCTGGGTTT GGTTTACCTG GGCTACCAGG CTCCCCAGGC
    CCTGCTGGAT TGAAAGGATC CCAAGGACCA AAAGGAGAAT CAGGCTTCCC AGGTTCTCCA
    GGCCTTCCTG GACGAGCAGG ATTTGATGGG CCTGTGGGAC CAAAAGGTGA CTCTGGACAA
    AATGGCTTAC CAGGAACCCC TGGACCACCT GGGGTACCTG GTGCTGGTGG TCAAGGCCCT
    CCAGGACAAC CAGGGCTTCC AGGGCCAGCA GGCCCTCCAG GTCCCACAGG GAACACTGGA
    ATACAGGGAG AGAAAGGAGA TCCTGGTGCT CCAGGGTTTG ACATGCCAGG CCCACCAGGA
    GATCGTGGTA GCCCTGGATT TCCAGGAGAA AGAGGGTTTA CAGGTCCACC AGGGCCACCT
    GGAGCCCAAG GTCGCGATGG ATTACCAGGC TTCTCAGGTG CTAAAGGTGA AATGGGTGTA
    ATGGGACCAC CAGGATCTGT CGGACCAGCT GGTAATCCTG GAAACCCTGG TTTACCTGGG
    TCTAAAGGAA ATGATGGGAA TCCCGGACAA CCTGGCCTGT CTGGTTTACC AGGGGCTGAA
    GGTGAAAAGG GAGAGAGAGG ATTACCAGGA CCCCCTGGAA GAGTTGATTC TGCAAACCTA
    CAGTTTAAAG GAGTTAAAGG AGAACCTGGC TTGCCAGGTT CATCAGGACT AAGTGGGGCA
    AAAGGTGCTG CTGGACTTCC TGGTAACTCA GGATCCCCAG GGCAGATTGG AATTCCAGGA
    TCTCCAGGTC TTCCAGGGCT CAAGGGAGAT TCTGGCCTTC CTGGTCAGCC GGGTACTTCA
    GGTCCTCCTG GATTAAAAGG GAGCATGGGA GAGATGGGAC TTCCTGGGCC TCCTGGTTCT
    AAAGGTTCCC CTGGACAGCC AGGAAGGCCT GGTTCAGCAG GGTCTCCTGG GTTTTCTGGA
    CTGAAAGGTG AAAAAGGTGA ACCAGGAATT TCAGGAGCTG GTATCCCAGG CTTTCCTGGG
    CAGAAGGGTG AACCAGGGCA AAATGGCTTT CCAGGCAGCC CTGGTAGTAA AGGCAACCCA
    GGAAACCCAG GACTGCCCGG CCTACCAGGG ATTTCAGGAC CTAAAGGGGA TCCAGGACTT
    CCAGGATTCC CAGGCAGCCC TGGATCTCCA GGCCTTAAAG GAGTTGATGG GCCCACAGGA
    AACTCTGGGT TACCCGGATC TCCAGGCTTA CGGGGTGAAT CAGGTCGTCC CGGACCTCAA
    GGAACTCCTG GTGAGAAGGG TCAACCAGGT CAGGATGGAA TTCCTGGGCC TTCTGGTTTG
    AAGGGAGAAC CAGGTTTGCC AGGTTTTGGA TCCCCAGGGC TGCCTGGCCT TCCAGGAAGT
    CAAGGTCAAA AGGGTGATGC AGGACTTTCT GGAAGCCCTG GAAACCCCGG ACTTCCAGGG
    TTCAAAGGAG ATCAAGGCTT TCCAGGATCT CCTGGGCCAC AAGGACCAAC AGGACAACCT
    GGCCTACCAG GGCCACGTAC AGAAGGACCC AAGGGAGACC CTGGGCCTGT AGGTCCCCCT
    GGAAGACCAG GTCCTCCTGG CTCAGAAGGT CCACGTGGTC CCCCTGGTGA TGGAGGCTTT
    AAAGGAGAAA AAGGAAACCC TGGATTTCCA GGTCAGCCTG GACTTACAGG ATCTAAAGGA
    GAACATGGAG CATCTGGTTT CCCAGGTGAC CCAGGACGAC CAGGTCTTAA TGGGATGAAA
    GGTGATCAAG GTGTTCCTGG TGTTCCAGGA TTCCCAGGTA TGAAAGGACC AGGTGGCCCT
    CCTGGATTGG CTGGCCTTAA AGGTGAATCC GGACTCCCAG GTGCTATAGG TTTGCCAGGT
    CTTCCTGGGC CTAAAGGAGA ATACCGTGAG ATAAAAGGAG CTCCAGGTCT GCCAGGTCCT
    CCAGGTCGCC CAGGAGTAAA TGGACCTCCA GGACCCCCCG GATCACCAGG ACTTCCAGGT
    TCTTTGGGTC TCCCTGGTGA TACTGGCAGA GATGGACTCC CTGGATTTGA GGGACAACTT
    GGACGAAAGG GTGACCGAGG CCTACCAGGC CAACCAGGAT CTCGTGGAAT TAATGGCCCA
    CCTGGCCCAG ATGGTTTACA AGGCCCCCCT GGCCCCCCTG GTCTAGGATC TGTTGCTCAT
    GGATTCCTTA TAACCCGACA CAGTCAGTCA ACAGATGCCC CTGGCTGTCC TTTTGGAACC
    ACCAGGATCT ATGATGGCTA TTCACTACTT TATGTGCAAG GAAATGAGAG GGCACACGGA
    CAAGATTTGG GTACCGCTGG CAGCTGCCTC CGTCGCTTCA GCACAATGCC TTTCATGTTC
    TGCAACATTA ATAACGTGTG TAACTTTGCT TCAAGAAATG ACTACTCCTA CTGGTTGTCC
    ACGCCTGAGC CTATGCCTAT GAGCATGGAA CCTTTAAGAG GGAGGGATAT CCAACCATTC
    ATTAGCAGGT GTGCGGTCTG TGAAGCGCCA GCCATGGTGA TTGCAGTTCA CAGCCAGACA
    ATACAAATCC CACAGTGCCC ACAAGGCTGG TCCTCTCTCT GGATTGGATA CTCTTTCATG
    ATGCATACAA GTGCCGGTGC TGAAGGCTCA GGACAAGCTC TGGCATCGCC TGGATCGTGC
    TTGGAGGAAT TCCGATCTGC ACCTTTCATT GAATGTCATG GCCGGGGAAC CTGCAATTAC
    TATGCCAATT CATACAGTTT TTGGTTGGCC ACAGTGGAAA TGTCTGAAAT GTTTAGCAAA
    CCTCAGTCAG AAACACTAAA AGCGGGTGAT CTAAGGACAC GCGTCAGTCG CTGCCAAGTT
    TGTATGAAGA GGACGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa65398
    Clone ID Related Accession (Same CDS sequence) XM_004916869.4
    Accession Version XM_004916869.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5049bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product collagen alpha-5(IV) chain isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004916869.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAGACTGG GAAGTTGGCA GCGGATGCCC TGCCTGGCTG TACTGGTGTT GGTGCTGGGG 
    CACTGGGCAC AGAATGCAGA GTCTGCGGCC TGTCAAGGTT GTACATCGGT ATCGAAATGT
    GACTGCAGTG GTGTTAAAGG AGAAAAGGGT GAGAGAGGCC TTCCAGGGTT AGAAGGACAC
    CTTGGTCTAC CAGGATTTCC TGGACCTGAA GGACCGCCTG GACCTCGTGG TCCCAAGGGT
    GATTCTGGTT TATCAGGGTC TCCAGGACCC AAAGGAATAC GAGGACCTCC TGGACCACCC
    GGATTTCCAG GAAACCCTGG CCAGCCTGGC CTGCCGGGAC ATGATGGTGA ACCAGGGCCT
    GCAGGTATCC CAGGATGCAA TGGCACAAAG GGAGATCGTG GTTTCCCAGG AGGTCCAGGT
    TTTTCGGGTA CACCAGGAAT ACCAGGTCCC CCAGGATTAC CAGGTGTGAA AGGAGATCCA
    GGTGAATCTT TCCCTTTAGC ACCGGGACAA AAAGGTGATC CAGGATTACC AGGTCTTCCA
    GGTTTACCAG GTCGGACTGG TCCTATTGGA GTTCAAGGTC CTGTGGGTCG ACCTGGTCCT
    GCTGGCTTAC CGGGGTCACC AGGAGTACCT GGACCTCCAG GACCCAAGGG TAACATGGGG
    CTGAATTTCC AGGGATCAAA GGGAGAAAAG GGTGAACCTG GTATTCAAGG ACTACCAGGA
    CCACCGGGAC AACTCGAGCA AGTTGAACCT CCACAAACTA CAATTCAGAA GGGAGAGAAG
    GGTATCCCCG GTGACCAAGG ATTTCCAGGA GAGCGTGGGC CAATGGGTCC TCCTGGTCCT
    CCTGGAGGTG CAAAGGGAGA GAAGGGAGAA CCAGGGGAAC CTGGAAAGAT TGGGAAGCCT
    GGAAAAGATG GAAAGATTGG TCTGCCCGGT GGAGAGGGTT TCAGGGGTGA CCCAGGCTTG
    CCGGGAGGTC CAGGCAGAGA TGGGGACAAG GGTCAAAAAG GTGAAAATGG GCTCCCTGGG
    CCACCAGGAG AGGTAATTTC AAGACCAGGG ACAGGCAGAG TTGGACCAAA AGGAAACACA
    GGACCCCCTG GAATAACAGG GCCAAAAGGA GACCGTGGAT TCCCTGGATT ACAAGGTCCC
    CCTGGTTTTC CTGGAACACC AGGTACTTCA AGTGTAGGCC CATCAGGAAA TCCAGGCCTT
    CCTGGTGAAA GAGGGCAAAA AGGCGATGAT GGTCCACCAG GAATCTCTAT TCCAGGGCTT
    CCAGGTTCTG AAGGTCTTGC AGGACCCCCT GGTCCCCCAG GTTTGCCTGG TCGACCTGGA
    ACATCTGCAG TTGGTCCTGC AATGGTATGC CAGCCAGGGC TACCTGGTAC TCAAGGACTT
    CCAGGAGATC CAGGATTTTT AGGAGAAAGA GGACAGAAAG GTGACAAAGG AGACACATGT
    TATAACTGCA TAGGAAATGG TATTTTAGGT CCGAAGGGGG ATCCTGGTTT GCCTGGTTTC
    CCTGGAAGTC CAGGTTCACC TGGAGCTCCA GGGCAAAAAG GAGCAATGGG TATCCCAGGA
    ACAAATGGGC TTCCAGGACC TCCGGGTTTT CCTGGCAATC CTGGTATCTC TGGCCGACCC
    GGTCCCAAAG GAGAACCTGG AGAAGTAATT GGCGAAATTA AAGGAGAAAA GGGGGACCCT
    GGATTCCCAG GTCCAGCTGG ATCTCCTGGT CTGGATGGTG TCCCTGGAAG AGACGGAACC
    CCAGGGATCC CTGGCCCTAA GGGAGAGCCG GGTGGCTTCG TGTTGAAAGG CGATCGTGGT
    TTTCCTGGTG ACATTGGGTT CCCTGGACAT CCTGGAGATC GAGGACTAAT GGGTCCTCCT
    GGTTTTGGTC CCAGTGGTCC ACCTGGAGAG AAGGGAATTC AAGGTGTTTC TGGTCGTCCA
    GGAGCCCCTG GAATACCTGG CCCTAAAGGA TCTCCTGGAC AGACTGTGAT GGAAAAAGGG
    GATTTTGGAC CCCCTGGTCT CCCAGGTATC CCAGGGGATG CCGGAACTCC AGGTGAACCA
    GGTCTACCTG GTCAGCCAGG AATTCCAGGT GTTCCAGGTT CAAAAGGAGA ACCAGGCATT
    CCAGGCATTG GACTTCCTGG GTCTGCAGGC GCAAAAGGAT TTCCTGGTAA TACTGGGCCA
    CCAGGTGCAC CAGGGTTGGC AGGAAGACCT GGTTCAGATG GGTCACCAGG ACAGCCTGGC
    TTGCCAGGAC CAAAGGGTGA CCCTGGGTTT GGTTTACCTG GGCTACCAGG CTCCCCAGGC
    CCTGCTGGAT TGAAAGGATC CCAAGGACCA AAAGGAGAAT CAGGCTTCCC AGGTTCTCCA
    GGCCTTCCTG GACGAGCAGG ATTTGATGGG CCTGTGGGAC CAAAAGGTGA CTCTGGACAA
    AATGGCTTAC CAGGAACCCC TGGACCACCT GGGGTACCTG GTGCTGGTGG TCAAGGCCCT
    CCAGGACAAC CAGGGCTTCC AGGGCCAGCA GGCCCTCCAG GGAACACTGG AATACAGGGA
    GAGAAAGGAG ATCCTGGTGC TCCAGGGTTT GACATGCCAG GCCCACCAGG AGATCGTGGT
    AGCCCTGGAT TTCCAGGAGA AAGAGGGTTT ACAGGTCCAC CAGGGCCACC TGGAGCCCAA
    GGTCGCGATG GATTACCAGG CTTCTCAGGT GCTAAAGGTG AAATGGGTGT AATGGGACCA
    CCAGGATCTG TCGGACCAGC TGGTAATCCT GGAAACCCTG GTTTACCTGG GTCTAAAGGA
    AATGATGGGA ATCCCGGACA ACCTGGCCTG TCTGGTTTAC CAGGGGCTGA AGGTGAAAAG
    GGAGAGAGAG GATTACCAGG ACCCCCTGGA AGAGTTGATT CTGCAAACCT ACAGTTTAAA
    GGAGTTAAAG GAGAACCTGG CTTGCCAGGT TCATCAGGAC TAAGTGGGGC AAAAGGTGCT
    GCTGGACTTC CTGGTAACTC AGGATCCCCA GGGCAGATTG GAATTCCAGG ATCTCCAGGT
    CTTCCAGGGC TCAAGGGAGA TTCTGGCCTT CCTGGTCAGC CGGGTACTTC AGGTCCTCCT
    GGATTAAAAG GGAGCATGGG AGAGATGGGA CTTCCTGGGC CTCCTGGTTC TAAAGGTTCC
    CCTGGACAGC CAGGAAGGCC TGGTTCAGCA GGGTCTCCTG GGTTTTCTGG ACTGAAAGGT
    GAAAAAGGTG AACCAGGAAT TTCAGGAGCT GGTATCCCAG GCTTTCCTGG GCAGAAGGGT
    GAACCAGGGC AAAATGGCTT TCCAGGCAGC CCTGGTAGTA AAGGCAACCC AGGAAACCCA
    GGACTGCCCG GCCTACCAGG GATTTCAGGA CCTAAAGGGG ATCCAGGACT TCCAGGATTC
    CCAGGCAGCC CTGGATCTCC AGGCCTTAAA GGAGTTGATG GGCCCACAGG AAACTCTGGG
    TTACCCGGAT CTCCAGGCTT ACGGGGTGAA TCAGGTCGTC CCGGACCTCA AGGAACTCCT
    GGTGAGAAGG GTCAACCAGG TCAGGATGGA ATTCCTGGGC CTTCTGGTTT GAAGGGAGAA
    CCAGGTTTGC CAGGTTTTGG ATCCCCAGGG CTGCCTGGCC TTCCAGGAAG TCAAGGTCAA
    AAGGGTGATG CAGGACTTTC TGGAAGCCCT GGAAACCCCG GACTTCCAGG GTTCAAAGGA
    GATCAAGGCT TTCCAGGATC TCCTGGGCCA CAAGGACCAA CAGGACAACC TGGCCTACCA
    GGGCCACGTA CAGAAGGACC CAAGGGAGAC CCTGGGCCTG TAGGTCCCCC TGGAAGACCA
    GGTCCTCCTG GCTCAGAAGG TCCACGTGGT CCCCCTGGTG ATGGAGGCTT TAAAGGAGAA
    AAAGGAAACC CTGGATTTCC AGGTCAGCCT GGACTTACAG GATCTAAAGG AGAACATGGA
    GCATCTGGTT TCCCAGGTGA CCCAGGACGA CCAGGTCTTA ATGGGATGAA AGGTGATCAA
    GGTGTTCCTG GTGTTCCAGG ATTCCCAGGT ATGAAAGGAC CAGGTGGCCC TCCTGGATTG
    GCTGGCCTTA AAGGTGAATC CGGACTCCCA GGTGCTATAG GTTTGCCAGG TCTTCCTGGG
    CCTAAAGGAG AATACCGTGA GATAAAAGGA GCTCCAGGTC TGCCAGGTCC TCCAGGTCGC
    CCAGGAGTAA ATGGACCTCC AGGACCCCCC GGATCACCAG GACTTCCAGG TTCTTTGGGT
    CTCCCTGGTG ATACTGGCAG AGATGGACTC CCTGGATTTG AGGGACAACT TGGACGAAAG
    GGTGACCGAG GCCTACCAGG CCAACCAGGA TCTCGTGGAA TTAATGGCCC ACCTGGCCCA
    GATGGTTTAC AAGGCCCCCC TGGCCCCCCT GGTCTAGGAT CTGTTGCTCA TGGATTCCTT
    ATAACCCGAC ACAGTCAGTC AACAGATGCC CCTGGCTGTC CTTTTGGAAC CACCAGGATC
    TATGATGGCT ATTCACTACT TTATGTGCAA GGAAATGAGA GGGCACACGG ACAAGATTTG
    GGTACCGCTG GCAGCTGCCT CCGTCGCTTC AGCACAATGC CTTTCATGTT CTGCAACATT
    AATAACGTGT GTAACTTTGC TTCAAGAAAT GACTACTCCT ACTGGTTGTC CACGCCTGAG
    CCTATGCCTA TGAGCATGGA ACCTTTAAGA GGGAGGGATA TCCAACCATT CATTAGCAGG
    TGTGCGGTCT GTGAAGCGCC AGCCATGGTG ATTGCAGTTC ACAGCCAGAC AATACAAATC
    CCACAGTGCC CACAAGGCTG GTCCTCTCTC TGGATTGGAT ACTCTTTCAT GATGCATACA
    AGTGCCGGTG CTGAAGGCTC AGGACAAGCT CTGGCATCGC CTGGATCGTG CTTGGAGGAA
    TTCCGATCTG CACCTTTCAT TGAATGTCAT GGCCGGGGAA CCTGCAATTA CTATGCCAAT
    TCATACAGTT TTTGGTTGGC CACAGTGGAA ATGTCTGAAA TGTTTAGCAA ACCTCAGTCA
    GAAACACTAA AAGCGGGTGA TCTAAGGACA CGCGTCAGTC GCTGCCAAGT TTGTATGAAG
    AGGACGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004916926.2
    CDS280..5328
    Translation

    Target ORF information:

    RefSeq Version XM_004916869.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis collagen, type IV, alpha 5 (col4a5), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004916869.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAGACTGG GAAGTTGGCA GCGGATGCCC TGCCTGGCTG TACTGGTGTT GGTGCTGGGG 
    CACTGGGCAC AGAATGCAGA GTCTGCGGCC TGTCAAGGTT GTACATCGGT ATCGAAATGT
    GACTGCAGTG GTGTTAAAGG AGAAAAGGGT GAGAGAGGCC TTCCAGGGTT AGAAGGACAC
    CTTGGTCTAC CAGGATTTCC TGGACCTGAA GGACCGCCTG GACCTCGTGG TCCCAAGGGT
    GATTCTGGTT TATCAGGGTC TCCAGGACCC AAAGGAATAC GAGGACCTCC TGGACCACCC
    GGATTTCCAG GAAACCCTGG CCAGCCTGGC CTGCCGGGAC ATGATGGTGA ACCAGGGCCT
    GCAGGTATCC CAGGATGCAA TGGCACAAAG GGAGATCGTG GTTTCCCAGG AGGTCCAGGT
    TTTTCGGGTA CACCAGGAAT ACCAGGTCCC CCAGGATTAC CAGGTGTGAA AGGAGATCCA
    GGTGAATCTT TCCCTTTAGC ACCGGGACAA AAAGGTGATC CAGGATTACC AGGTCTTCCA
    GGTTTACCAG GTCGGACTGG TCCTATTGGA GTTCAAGGTC CTGTGGGTCG ACCTGGTCCT
    GCTGGCTTAC CGGGGTCACC AGGAGTACCT GGACCTCCAG GACCCAAGGG TAACATGGGG
    CTGAATTTCC AGGGATCAAA GGGAGAAAAG GGTGAACCTG GTATTCAAGG ACTACCAGGA
    CCACCGGGAC AACTCGAGCA AGTTGAACCT CCACAAACTA CAATTCAGAA GGGAGAGAAG
    GGTATCCCCG GTGACCAAGG ATTTCCAGGA GAGCGTGGGC CAATGGGTCC TCCTGGTCCT
    CCTGGAGGTG CAAAGGGAGA GAAGGGAGAA CCAGGGGAAC CTGGAAAGAT TGGGAAGCCT
    GGAAAAGATG GAAAGATTGG TCTGCCCGGT GGAGAGGGTT TCAGGGGTGA CCCAGGCTTG
    CCGGGAGGTC CAGGCAGAGA TGGGGACAAG GGTCAAAAAG GTGAAAATGG GCTCCCTGGG
    CCACCAGGAG AGGTAATTTC AAGACCAGGG ACAGGCAGAG TTGGACCAAA AGGAAACACA
    GGACCCCCTG GAATAACAGG GCCAAAAGGA GACCGTGGAT TCCCTGGATT ACAAGGTCCC
    CCTGGTTTTC CTGGAACACC AGGTACTTCA AGTGTAGGCC CATCAGGAAA TCCAGGCCTT
    CCTGGTGAAA GAGGGCAAAA AGGCGATGAT GGTCCACCAG GAATCTCTAT TCCAGGGCTT
    CCAGGTTCTG AAGGTCTTGC AGGACCCCCT GGTCCCCCAG GTTTGCCTGG TCGACCTGGA
    ACATCTGCAG TTGGTCCTGC AATGGTATGC CAGCCAGGGC TACCTGGTAC TCAAGGACTT
    CCAGGAGATC CAGGATTTTT AGGAGAAAGA GGACAGAAAG GTGACAAAGG AGACACATGT
    TATAACTGCA TAGGAAATGG TATTTTAGGT CCGAAGGGGG ATCCTGGTTT GCCTGGTTTC
    CCTGGAAGTC CAGGTTCACC TGGAGCTCCA GGGCAAAAAG GAGCAATGGG TATCCCAGGA
    ACAAATGGGC TTCCAGGACC TCCGGGTTTT CCTGGCAATC CTGGTATCTC TGGCCGACCC
    GGTCCCAAAG GAGAACCTGG AGAAGTAATT GGCGAAATTA AAGGAGAAAA GGGGGACCCT
    GGATTCCCAG GTCCAGCTGG ATCTCCTGGT CTGGATGGTG TCCCTGGAAG AGACGGAACC
    CCAGGGATCC CTGGCCCTAA GGGAGAGCCG GGTGGCTTCG TGTTGAAAGG CGATCGTGGT
    TTTCCTGGTG ACATTGGGTT CCCTGGACAT CCTGGAGATC GAGGACTAAT GGGTCCTCCT
    GGTTTTGGTC CCAGTGGTCC ACCTGGAGAG AAGGGAATTC AAGGTGTTTC TGGTCGTCCA
    GGAGCCCCTG GAATACCTGG CCCTAAAGGA TCTCCTGGAC AGACTGTGAT GGAAAAAGGG
    GATTTTGGAC CCCCTGGTCT CCCAGGTATC CCAGGGGATG CCGGAACTCC AGGTGAACCA
    GGTCTACCTG GTCAGCCAGG AATTCCAGGT GTTCCAGGTT CAAAAGGAGA ACCAGGCATT
    CCAGGCATTG GACTTCCTGG GTCTGCAGGC GCAAAAGGAT TTCCTGGTAA TACTGGGCCA
    CCAGGTGCAC CAGGGTTGGC AGGAAGACCT GGTTCAGATG GGTCACCAGG ACAGCCTGGC
    TTGCCAGGAC CAAAGGGTGA CCCTGGGTTT GGTTTACCTG GGCTACCAGG CTCCCCAGGC
    CCTGCTGGAT TGAAAGGATC CCAAGGACCA AAAGGAGAAT CAGGCTTCCC AGGTTCTCCA
    GGCCTTCCTG GACGAGCAGG ATTTGATGGG CCTGTGGGAC CAAAAGGTGA CTCTGGACAA
    AATGGCTTAC CAGGAACCCC TGGACCACCT GGGGTACCTG GTGCTGGTGG TCAAGGCCCT
    CCAGGACAAC CAGGGCTTCC AGGGCCAGCA GGCCCTCCAG GGAACACTGG AATACAGGGA
    GAGAAAGGAG ATCCTGGTGC TCCAGGGTTT GACATGCCAG GCCCACCAGG AGATCGTGGT
    AGCCCTGGAT TTCCAGGAGA AAGAGGGTTT ACAGGTCCAC CAGGGCCACC TGGAGCCCAA
    GGTCGCGATG GATTACCAGG CTTCTCAGGT GCTAAAGGTG AAATGGGTGT AATGGGACCA
    CCAGGATCTG TCGGACCAGC TGGTAATCCT GGAAACCCTG GTTTACCTGG GTCTAAAGGA
    AATGATGGGA ATCCCGGACA ACCTGGCCTG TCTGGTTTAC CAGGGGCTGA AGGTGAAAAG
    GGAGAGAGAG GATTACCAGG ACCCCCTGGA AGAGTTGATT CTGCAAACCT ACAGTTTAAA
    GGAGTTAAAG GAGAACCTGG CTTGCCAGGT TCATCAGGAC TAAGTGGGGC AAAAGGTGCT
    GCTGGACTTC CTGGTAACTC AGGATCCCCA GGGCAGATTG GAATTCCAGG ATCTCCAGGT
    CTTCCAGGGC TCAAGGGAGA TTCTGGCCTT CCTGGTCAGC CGGGTACTTC AGGTCCTCCT
    GGATTAAAAG GGAGCATGGG AGAGATGGGA CTTCCTGGGC CTCCTGGTTC TAAAGGTTCC
    CCTGGACAGC CAGGAAGGCC TGGTTCAGCA GGGTCTCCTG GGTTTTCTGG ACTGAAAGGT
    GAAAAAGGTG AACCAGGAAT TTCAGGAGCT GGTATCCCAG GCTTTCCTGG GCAGAAGGGT
    GAACCAGGGC AAAATGGCTT TCCAGGCAGC CCTGGTAGTA AAGGCAACCC AGGAAACCCA
    GGACTGCCCG GCCTACCAGG GATTTCAGGA CCTAAAGGGG ATCCAGGACT TCCAGGATTC
    CCAGGCAGCC CTGGATCTCC AGGCCTTAAA GGAGTTGATG GGCCCACAGG AAACTCTGGG
    TTACCCGGAT CTCCAGGCTT ACGGGGTGAA TCAGGTCGTC CCGGACCTCA AGGAACTCCT
    GGTGAGAAGG GTCAACCAGG TCAGGATGGA ATTCCTGGGC CTTCTGGTTT GAAGGGAGAA
    CCAGGTTTGC CAGGTTTTGG ATCCCCAGGG CTGCCTGGCC TTCCAGGAAG TCAAGGTCAA
    AAGGGTGATG CAGGACTTTC TGGAAGCCCT GGAAACCCCG GACTTCCAGG GTTCAAAGGA
    GATCAAGGCT TTCCAGGATC TCCTGGGCCA CAAGGACCAA CAGGACAACC TGGCCTACCA
    GGGCCACGTA CAGAAGGACC CAAGGGAGAC CCTGGGCCTG TAGGTCCCCC TGGAAGACCA
    GGTCCTCCTG GCTCAGAAGG TCCACGTGGT CCCCCTGGTG ATGGAGGCTT TAAAGGAGAA
    AAAGGAAACC CTGGATTTCC AGGTCAGCCT GGACTTACAG GATCTAAAGG AGAACATGGA
    GCATCTGGTT TCCCAGGTGA CCCAGGACGA CCAGGTCTTA ATGGGATGAA AGGTGATCAA
    GGTGTTCCTG GTGTTCCAGG ATTCCCAGGT ATGAAAGGAC CAGGTGGCCC TCCTGGATTG
    GCTGGCCTTA AAGGTGAATC CGGACTCCCA GGTGCTATAG GTTTGCCAGG TCTTCCTGGG
    CCTAAAGGAG AATACCGTGA GATAAAAGGA GCTCCAGGTC TGCCAGGTCC TCCAGGTCGC
    CCAGGAGTAA ATGGACCTCC AGGACCCCCC GGATCACCAG GACTTCCAGG TTCTTTGGGT
    CTCCCTGGTG ATACTGGCAG AGATGGACTC CCTGGATTTG AGGGACAACT TGGACGAAAG
    GGTGACCGAG GCCTACCAGG CCAACCAGGA TCTCGTGGAA TTAATGGCCC ACCTGGCCCA
    GATGGTTTAC AAGGCCCCCC TGGCCCCCCT GGTCTAGGAT CTGTTGCTCA TGGATTCCTT
    ATAACCCGAC ACAGTCAGTC AACAGATGCC CCTGGCTGTC CTTTTGGAAC CACCAGGATC
    TATGATGGCT ATTCACTACT TTATGTGCAA GGAAATGAGA GGGCACACGG ACAAGATTTG
    GGTACCGCTG GCAGCTGCCT CCGTCGCTTC AGCACAATGC CTTTCATGTT CTGCAACATT
    AATAACGTGT GTAACTTTGC TTCAAGAAAT GACTACTCCT ACTGGTTGTC CACGCCTGAG
    CCTATGCCTA TGAGCATGGA ACCTTTAAGA GGGAGGGATA TCCAACCATT CATTAGCAGG
    TGTGCGGTCT GTGAAGCGCC AGCCATGGTG ATTGCAGTTC ACAGCCAGAC AATACAAATC
    CCACAGTGCC CACAAGGCTG GTCCTCTCTC TGGATTGGAT ACTCTTTCAT GATGCATACA
    AGTGCCGGTG CTGAAGGCTC AGGACAAGCT CTGGCATCGC CTGGATCGTG CTTGGAGGAA
    TTCCGATCTG CACCTTTCAT TGAATGTCAT GGCCGGGGAA CCTGCAATTA CTATGCCAAT
    TCATACAGTT TTTGGTTGGC CACAGTGGAA ATGTCTGAAA TGTTTAGCAA ACCTCAGTCA
    GAAACACTAA AAGCGGGTGA TCTAAGGACA CGCGTCAGTC GCTGCCAAGT TTGTATGAAG
    AGGACGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa14360
    Clone ID Related Accession (Same CDS sequence) XM_004916865.3
    Accession Version XM_004916865.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5076bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product collagen alpha-5(IV) chain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004916865.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAGACTGG GAAGTTGGCA GCGGATGCCC TGCCTGGCTG TACTGGTGTT GGTGCTGGGG 
    CACTGGGCAC AGAATGCAGA GTCTGCGGCC TGTCAAGGTT GTACATCGGT ATCGAAATGT
    GACTGCAGTG GTGTTAAAGG AGAAAAGGGT GAGAGAGGCC TTCCAGGGTT AGAAGGACAC
    CTTGGTCTAC CAGGATTTCC TGGACCTGAA GGACCGCCTG GACCTCGTGG TCCCAAGGGT
    GATTCTGGTT TATCAGGGTC TCCAGGACCC AAAGGAATAC GAGGACCTCC TGGACCACCC
    GGATTTCCAG GAAACCCTGG CCAGCCTGGC CTGCCGGGAC ATGATGGTGA ACCAGGGCCT
    GCAGGTATCC CAGGATGCAA TGGCACAAAG GGAGATCGTG GTTTCCCAGG AGGTCCAGGT
    TTTTCGGGTA CACCAGGAAT ACCAGGTCCC CCAGGATTAC CAGGTGTGAA AGGAGATCCA
    GGTGAATCTT TCCCTTTAGC ACCGGGACAA AAAGGTGATC CAGGATTACC AGGTCTTCCA
    GGTTTACCAG GTCGGACTGG TCCTATTGGA GTTCAAGGTC CTGTGGGTCG ACCTGGTCCT
    GCTGGCTTAC CGGGGTCACC AGGAGTACCT GGACCTCCAG GACCCAAGGG TAACATGGGG
    CTGAATTTCC AGGGATCAAA GGGAGAAAAG GGTGAACCTG GTATTCAAGG ACTACCAGGA
    CCACCGGGAC AACTCGAGCA AGTTGAACCT CCACAAACTA CAATTCAGAA GGGAGAGAAG
    GGTATCCCCG GTGACCAAGG ATTTCCAGGA GAGCGTGGGC CAATGGGTCC TCCTGGTCCT
    CCTGGAGGTG CAAAGGGAGA GAAGGGAGAA CCAGGGGAAC CTGGAAAGAT TGGGAAGCCT
    GGAAAAGATG GAAAGATTGG TCTGCCCGGT GGAGAGGGTT TCAGGGGTGA CCCAGGCTTG
    CCGGGAGGTC CAGGCAGAGA TGGGGACAAG GGTCAAAAAG GTGAAAATGG GCTCCCTGGG
    CCACCAGGAG AGGTAATTTC AAGACCAGGG ACAGGCAGAG TTGGACCAAA AGGAAACACA
    GGACCCCCTG GAATAACAGG GCCAAAAGGA GACCGTGGAT TCCCTGGATT ACAAGGTCCC
    CCTGGTTTTC CTGGAACACC AGGTACTTCA AGTGTAGGCC CATCAGGAAA TCCAGGCCTT
    CCTGGTGAAA GAGGGCAAAA AGGCGATGAT GGTCCACCAG GAATCTCTAT TCCAGGGCTT
    CCAGGTTCTG AAGGTCTTGC AGGACCCCCT GGTCCCCCAG GTTTGCCTGG TCGACCTGGA
    ACATCTGCAG TTGGTCCTGC AATGGTATGC CAGCCAGGGC TACCTGGTAC TCAAGGACTT
    CCAGGAGATC CAGGATTTTT AGGAGAAAGA GGACAGAAAG GTGACAAAGG AGACACATGT
    TATAACTGCA TAGGAAATGG TATTTTAGGT CCGAAGGGGG ATCCTGGTTT GCCTGGTTTC
    CCTGGAAGTC CAGGTTCACC TGGAGCTCCA GGGCAAAAAG GAGCAATGGG TATCCCAGGA
    ACAAATGGGC TTCCAGGACC TCCGGGTTTT CCTGGCAATC CTGGTATCTC TGGCCGACCC
    GGTCCCAAAG GAGAACCTGG AGAAGTAATT GGCGAAATTA AAGGAGAAAA GGGGGACCCT
    GGATTCCCAG GTCCAGCTGG ATCTCCTGGT CTGGATGGTG TCCCTGGAAG AGACGGAACC
    CCAGGGATCC CTGGCCCTAA GGGAGAGCCG GGTGGCTTCG CGTTGAAAGG CGATCGTGGT
    TTTCCTGGTG ACATTGGGTT CCCTGGACAT CCTGGAGATC GAGGACTAAT GGGTCCTCCT
    GGTTTTGGTC CCAGTGGTCC ACCTGGAGAG AAGGGAATTC AAGGTGTTTC TGGTCGTCCA
    GGAGCCCCTG GAATACCTGG CCCTAAAGGA TCTCCTGGAC AGACTGTGAT GGAAAAAGGG
    GATTTTGGAC CCCCTGGTCT CCCAGGTATC CCAGGGGATG CCGGAACTCC AGGTGAACCA
    GGTCTACCTG GTCAGCCAGG AATTCCAGGT GTTCCAGGTT CAAAAGGAGA ACCAGGCATT
    CCAGGCATTG GACTTCCTGG GTCTGCAGGC GCAAAAGGAT TTCCTGGTAA TACTGGGCCA
    CCAGGTGCAC CAGGGTTGGC AGGAAGACCT GGTTCAGATG GGTCACCAGG ACAGCCTGGC
    TTGCCAGGAC CAAAGGGTGA CCCTGGGTTT GGTTTACCTG GGCTACCAGG CTCCCCAGGC
    CCTGCTGGAT TGAAAGGATC CCAAGGACCA AAAGGAGAAT CAGGCTTCCC AGGTTCTCCA
    GGCCTTCCTG GACGAGCAGG ATTTGATGGG CCTGTGGGAC CAAAAGGTGA CTCTGGACAA
    AATGGCTTAC CAGGAACCCC TGGACCACCT GGGGTACCTG GTGCTGGTGG TCAAGGCCCT
    CCAGGACAAC CAGGGCTTCC AGGGCCAGCA GGCCCTCCAG GTCCCACAGG GAACACTGGA
    ATACAGGGAG AGAAAGGAGA TCCTGGTGCT CCAGGGTTTG ACATGCCAGG CCCACCAGGA
    GATCGTGGTA GCCCTGGATT TCCAGGAGAA AGAGGGTTTA CAGGACCACC AGGGCCACCT
    GGAGCCCAAG GTCGCGATGG ATTACCAGGC TTCTCAGGTG CTAAAGGTGA AATGGGTGTA
    ATGGGACCAC CAGGATCTGT CGGACCAGCT GGTAATCCTG GAAACCCTGG TTTACCTGGG
    TCTAAAGGAA ATGATGGGAA TCCTGGACAA CCTGGCCTGT CTGGTTTACC AGGGGCTGAA
    GGTGAAAAGG GAGAGAGAGG ATTACCAGGA CCCCCTGGAA GAGTTGATTC TGCAAACCTA
    CAGTTTAAAG GAGTTAAAGG AGAACCTGGC TTGCCAGGTT CATCAGGACT AAGTGGGGCA
    AAAGGTGCTG CTGGACTTCC TGGTAACTCA GGATCCCCAG GGCAGATTGG AATTCCAGGA
    TCTCCAGGTC TTCCAGGGCT CAAGGGAGAT TCTGGCCTTC CTGGTCAGCC GGGTACTTCA
    GGTCCTCCTG GATTAAAAGG GAGCATGGGA GAGATGGGAC TTCCTGGGCC TCCTGGTTCT
    AAAGGTTCCC CTGGACAGCC AGGAAGGCCT GGTTCAGCAG GGTCTCCTGG GTTTTCTGGA
    CTGAAAGGTG AAAAAGGTGA ACCAGGAATT TCAGGAGCTG GTATCCCAGG CTTTCCTGGG
    CAGAAGGGTG AACCAGGGCA AAATGGCTTT CCAGGCAGCC CTGGTAGTAA AGGCAACCCA
    GGAAACCCAG GACTGCCCGG CCTACCAGGG ATTTCAGGAC CTAAAGGGGA TCCAGGACTT
    CCAGGATTCC CAGGCAGCCC TGGATCTCCA GGCCTTAAAG GAGTTGATGG GCCCACAGGA
    AACTCTGGGT TACCCGGATC TCCAGGCTTA CGGGGTGAAT CAGGTCGTCC CGGACCTCAA
    GGAACTCCTG GTGAGAAGGG TCAACCAGGT CAGGATGGAA TTCCTGGGCC TTCTGGTTTG
    AAGGGAGAAC CAGGTTTGCC AGGTTTTGGA TCCCCAGGGC TGCCTGGCCT TCCAGGAAGT
    CAAGGTCAAA AGGGTGATGC AGGACTTTCT GGAAGCCCTG GAAACCCCGG ACTTCCAGGG
    TTCAAAGGAG ATCAAGGCTT TCCAGGATCT CCTGGGCCAC AAGGACCAAC AGGACAACCT
    GGCCTACCAG GGCCACGTAC AGAAGGACCC AAGGGAGACC CTGGGCCTGT AGGTCCCCCT
    GGAAGACCAG GCCCTCAAGG TTATCCAGGT CCTCCTGGCT CAGAAGGTCC ACGTGGTCCC
    CCTGGTGATG GAGGCTTTAA AGGAGAAAAA GGAAACCCTG GATTTCCAGG TCAGCCTGGA
    CTTACAGGAT CTAAAGGAGA ACATGGAGCA TCTGGTTTCC CAGGTGACCC AGGACGACCA
    GGTCTTAATG GGATGAAAGG TGATCAAGGT GTTCCTGGTG TTCCAGGATT CCCAGGTATG
    AAAGGACCAG GTGGCCCTCC TGGATTGGCT GGCCTTAAAG GTGAATCCGG ACTCCCAGGT
    GCTATAGGTT TGCCAGGTCT TCCTGGGCCT AAAGGAGAAT ACCGTGAGAT AAAAGGAGCT
    CCAGGTCTGC CAGGTCCTCC AGGTCGCCCA GGAGTAAATG GACCTCCAGG ACCCCCCGGA
    TCACCAGGAC TTCCAGGTTC TTTGGGTCTC CCTGGTGATA CTGGCAGAGA TGGACTCCCT
    GGATTTGAGG GACAACTTGG ACGAAAGGGT GACCGAGGCC TACCAGGCCA ACCAGGATCT
    CGTGGAATTA ATGGCCCACC TGGCCCAGAT GGTTTACAAG GCCCCCCTGG CCCCCCTGGT
    CTAGGATCTG TTGCTCATGG ATTCCTTATA ACCCGACACA GTCAGTCAAC AGATGCCCCT
    GGCTGTCCTT TTGGAACCAC CAGGATCTAT GATGGCTATT CACTACTTTA TGTGCAAGGA
    AATGAGAGGG CACACGGACA AGATTTGGGT ACCGCTGGCA GCTGCCTCCG TCGCTTCAGC
    ACAATGCCTT TCATGTTCTG CAACATTAAT AACGTGTGTA ACTTTGCTTC AAGAAATGAC
    TACTCCTACT GGTTGTCCAC GCCTGAGCCT ATGCCTATGA GCATGGAACC TTTAAGAGGG
    AGGGATATCC AACCATTCAT TAGCAGGTGT GCGGTCTGTG AAGCGCCAGC CATGGTGATT
    GCAGTTCACA GCCAGACAAT ACAAATCCCA CAGTGCCCAC AAGGCTGGTC CTCTCTCTGG
    ATTGGATACT CTTTCATGAT GCATACAAGT GCCGGTGCTG AAGGCTCAGG ACAAGCTCTG
    GCATCGCCTG GATCGTGCTT GGAGGAATTC CGATCTGCAC CTTTCATTGA ATGTCATGGC
    CGGGGAACCT GCAATTACTA TGCCAATTCA TACAGTTTTT GGTTGGCCAC AGTGGAAATG
    TCTGAAATGT TTAGCAAACC TCAGTCAGAA ACACTAAAAG CGGGTGATCT AAGGACACGC
    GTCAGTCGCT GCCAAGTTTG TATGAAGAGG ACGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004916922.1
    CDS289..5364
    Translation

    Target ORF information:

    RefSeq Version XM_004916865.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis collagen, type IV, alpha 5 (col4a5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004916865.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAGACTGG GAAGTTGGCA GCGGATGCCC TGCCTGGCTG TACTGGTGTT GGTGCTGGGG 
    CACTGGGCAC AGAATGCAGA GTCTGCGGCC TGTCAAGGTT GTACATCGGT ATCGAAATGT
    GACTGCAGTG GTGTTAAAGG AGAAAAGGGT GAGAGAGGCC TTCCAGGGTT AGAAGGACAC
    CTTGGTCTAC CAGGATTTCC TGGACCTGAA GGACCGCCTG GACCTCGTGG TCCCAAGGGT
    GATTCTGGTT TATCAGGGTC TCCAGGACCC AAAGGAATAC GAGGACCTCC TGGACCACCC
    GGATTTCCAG GAAACCCTGG CCAGCCTGGC CTGCCGGGAC ATGATGGTGA ACCAGGGCCT
    GCAGGTATCC CAGGATGCAA TGGCACAAAG GGAGATCGTG GTTTCCCAGG AGGTCCAGGT
    TTTTCGGGTA CACCAGGAAT ACCAGGTCCC CCAGGATTAC CAGGTGTGAA AGGAGATCCA
    GGTGAATCTT TCCCTTTAGC ACCGGGACAA AAAGGTGATC CAGGATTACC AGGTCTTCCA
    GGTTTACCAG GTCGGACTGG TCCTATTGGA GTTCAAGGTC CTGTGGGTCG ACCTGGTCCT
    GCTGGCTTAC CGGGGTCACC AGGAGTACCT GGACCTCCAG GACCCAAGGG TAACATGGGG
    CTGAATTTCC AGGGATCAAA GGGAGAAAAG GGTGAACCTG GTATTCAAGG ACTACCAGGA
    CCACCGGGAC AACTCGAGCA AGTTGAACCT CCACAAACTA CAATTCAGAA GGGAGAGAAG
    GGTATCCCCG GTGACCAAGG ATTTCCAGGA GAGCGTGGGC CAATGGGTCC TCCTGGTCCT
    CCTGGAGGTG CAAAGGGAGA GAAGGGAGAA CCAGGGGAAC CTGGAAAGAT TGGGAAGCCT
    GGAAAAGATG GAAAGATTGG TCTGCCCGGT GGAGAGGGTT TCAGGGGTGA CCCAGGCTTG
    CCGGGAGGTC CAGGCAGAGA TGGGGACAAG GGTCAAAAAG GTGAAAATGG GCTCCCTGGG
    CCACCAGGAG AGGTAATTTC AAGACCAGGG ACAGGCAGAG TTGGACCAAA AGGAAACACA
    GGACCCCCTG GAATAACAGG GCCAAAAGGA GACCGTGGAT TCCCTGGATT ACAAGGTCCC
    CCTGGTTTTC CTGGAACACC AGGTACTTCA AGTGTAGGCC CATCAGGAAA TCCAGGCCTT
    CCTGGTGAAA GAGGGCAAAA AGGCGATGAT GGTCCACCAG GAATCTCTAT TCCAGGGCTT
    CCAGGTTCTG AAGGTCTTGC AGGACCCCCT GGTCCCCCAG GTTTGCCTGG TCGACCTGGA
    ACATCTGCAG TTGGTCCTGC AATGGTATGC CAGCCAGGGC TACCTGGTAC TCAAGGACTT
    CCAGGAGATC CAGGATTTTT AGGAGAAAGA GGACAGAAAG GTGACAAAGG AGACACATGT
    TATAACTGCA TAGGAAATGG TATTTTAGGT CCGAAGGGGG ATCCTGGTTT GCCTGGTTTC
    CCTGGAAGTC CAGGTTCACC TGGAGCTCCA GGGCAAAAAG GAGCAATGGG TATCCCAGGA
    ACAAATGGGC TTCCAGGACC TCCGGGTTTT CCTGGCAATC CTGGTATCTC TGGCCGACCC
    GGTCCCAAAG GAGAACCTGG AGAAGTAATT GGCGAAATTA AAGGAGAAAA GGGGGACCCT
    GGATTCCCAG GTCCAGCTGG ATCTCCTGGT CTGGATGGTG TCCCTGGAAG AGACGGAACC
    CCAGGGATCC CTGGCCCTAA GGGAGAGCCG GGTGGCTTCG CGTTGAAAGG CGATCGTGGT
    TTTCCTGGTG ACATTGGGTT CCCTGGACAT CCTGGAGATC GAGGACTAAT GGGTCCTCCT
    GGTTTTGGTC CCAGTGGTCC ACCTGGAGAG AAGGGAATTC AAGGTGTTTC TGGTCGTCCA
    GGAGCCCCTG GAATACCTGG CCCTAAAGGA TCTCCTGGAC AGACTGTGAT GGAAAAAGGG
    GATTTTGGAC CCCCTGGTCT CCCAGGTATC CCAGGGGATG CCGGAACTCC AGGTGAACCA
    GGTCTACCTG GTCAGCCAGG AATTCCAGGT GTTCCAGGTT CAAAAGGAGA ACCAGGCATT
    CCAGGCATTG GACTTCCTGG GTCTGCAGGC GCAAAAGGAT TTCCTGGTAA TACTGGGCCA
    CCAGGTGCAC CAGGGTTGGC AGGAAGACCT GGTTCAGATG GGTCACCAGG ACAGCCTGGC
    TTGCCAGGAC CAAAGGGTGA CCCTGGGTTT GGTTTACCTG GGCTACCAGG CTCCCCAGGC
    CCTGCTGGAT TGAAAGGATC CCAAGGACCA AAAGGAGAAT CAGGCTTCCC AGGTTCTCCA
    GGCCTTCCTG GACGAGCAGG ATTTGATGGG CCTGTGGGAC CAAAAGGTGA CTCTGGACAA
    AATGGCTTAC CAGGAACCCC TGGACCACCT GGGGTACCTG GTGCTGGTGG TCAAGGCCCT
    CCAGGACAAC CAGGGCTTCC AGGGCCAGCA GGCCCTCCAG GTCCCACAGG GAACACTGGA
    ATACAGGGAG AGAAAGGAGA TCCTGGTGCT CCAGGGTTTG ACATGCCAGG CCCACCAGGA
    GATCGTGGTA GCCCTGGATT TCCAGGAGAA AGAGGGTTTA CAGGACCACC AGGGCCACCT
    GGAGCCCAAG GTCGCGATGG ATTACCAGGC TTCTCAGGTG CTAAAGGTGA AATGGGTGTA
    ATGGGACCAC CAGGATCTGT CGGACCAGCT GGTAATCCTG GAAACCCTGG TTTACCTGGG
    TCTAAAGGAA ATGATGGGAA TCCTGGACAA CCTGGCCTGT CTGGTTTACC AGGGGCTGAA
    GGTGAAAAGG GAGAGAGAGG ATTACCAGGA CCCCCTGGAA GAGTTGATTC TGCAAACCTA
    CAGTTTAAAG GAGTTAAAGG AGAACCTGGC TTGCCAGGTT CATCAGGACT AAGTGGGGCA
    AAAGGTGCTG CTGGACTTCC TGGTAACTCA GGATCCCCAG GGCAGATTGG AATTCCAGGA
    TCTCCAGGTC TTCCAGGGCT CAAGGGAGAT TCTGGCCTTC CTGGTCAGCC GGGTACTTCA
    GGTCCTCCTG GATTAAAAGG GAGCATGGGA GAGATGGGAC TTCCTGGGCC TCCTGGTTCT
    AAAGGTTCCC CTGGACAGCC AGGAAGGCCT GGTTCAGCAG GGTCTCCTGG GTTTTCTGGA
    CTGAAAGGTG AAAAAGGTGA ACCAGGAATT TCAGGAGCTG GTATCCCAGG CTTTCCTGGG
    CAGAAGGGTG AACCAGGGCA AAATGGCTTT CCAGGCAGCC CTGGTAGTAA AGGCAACCCA
    GGAAACCCAG GACTGCCCGG CCTACCAGGG ATTTCAGGAC CTAAAGGGGA TCCAGGACTT
    CCAGGATTCC CAGGCAGCCC TGGATCTCCA GGCCTTAAAG GAGTTGATGG GCCCACAGGA
    AACTCTGGGT TACCCGGATC TCCAGGCTTA CGGGGTGAAT CAGGTCGTCC CGGACCTCAA
    GGAACTCCTG GTGAGAAGGG TCAACCAGGT CAGGATGGAA TTCCTGGGCC TTCTGGTTTG
    AAGGGAGAAC CAGGTTTGCC AGGTTTTGGA TCCCCAGGGC TGCCTGGCCT TCCAGGAAGT
    CAAGGTCAAA AGGGTGATGC AGGACTTTCT GGAAGCCCTG GAAACCCCGG ACTTCCAGGG
    TTCAAAGGAG ATCAAGGCTT TCCAGGATCT CCTGGGCCAC AAGGACCAAC AGGACAACCT
    GGCCTACCAG GGCCACGTAC AGAAGGACCC AAGGGAGACC CTGGGCCTGT AGGTCCCCCT
    GGAAGACCAG GCCCTCAAGG TTATCCAGGT CCTCCTGGCT CAGAAGGTCC ACGTGGTCCC
    CCTGGTGATG GAGGCTTTAA AGGAGAAAAA GGAAACCCTG GATTTCCAGG TCAGCCTGGA
    CTTACAGGAT CTAAAGGAGA ACATGGAGCA TCTGGTTTCC CAGGTGACCC AGGACGACCA
    GGTCTTAATG GGATGAAAGG TGATCAAGGT GTTCCTGGTG TTCCAGGATT CCCAGGTATG
    AAAGGACCAG GTGGCCCTCC TGGATTGGCT GGCCTTAAAG GTGAATCCGG ACTCCCAGGT
    GCTATAGGTT TGCCAGGTCT TCCTGGGCCT AAAGGAGAAT ACCGTGAGAT AAAAGGAGCT
    CCAGGTCTGC CAGGTCCTCC AGGTCGCCCA GGAGTAAATG GACCTCCAGG ACCCCCCGGA
    TCACCAGGAC TTCCAGGTTC TTTGGGTCTC CCTGGTGATA CTGGCAGAGA TGGACTCCCT
    GGATTTGAGG GACAACTTGG ACGAAAGGGT GACCGAGGCC TACCAGGCCA ACCAGGATCT
    CGTGGAATTA ATGGCCCACC TGGCCCAGAT GGTTTACAAG GCCCCCCTGG CCCCCCTGGT
    CTAGGATCTG TTGCTCATGG ATTCCTTATA ACCCGACACA GTCAGTCAAC AGATGCCCCT
    GGCTGTCCTT TTGGAACCAC CAGGATCTAT GATGGCTATT CACTACTTTA TGTGCAAGGA
    AATGAGAGGG CACACGGACA AGATTTGGGT ACCGCTGGCA GCTGCCTCCG TCGCTTCAGC
    ACAATGCCTT TCATGTTCTG CAACATTAAT AACGTGTGTA ACTTTGCTTC AAGAAATGAC
    TACTCCTACT GGTTGTCCAC GCCTGAGCCT ATGCCTATGA GCATGGAACC TTTAAGAGGG
    AGGGATATCC AACCATTCAT TAGCAGGTGT GCGGTCTGTG AAGCGCCAGC CATGGTGATT
    GCAGTTCACA GCCAGACAAT ACAAATCCCA CAGTGCCCAC AAGGCTGGTC CTCTCTCTGG
    ATTGGATACT CTTTCATGAT GCATACAAGT GCCGGTGCTG AAGGCTCAGG ACAAGCTCTG
    GCATCGCCTG GATCGTGCTT GGAGGAATTC CGATCTGCAC CTTTCATTGA ATGTCATGGC
    CGGGGAACCT GCAATTACTA TGCCAATTCA TACAGTTTTT GGTTGGCCAC AGTGGAAATG
    TCTGAAATGT TTAGCAAACC TCAGTCAGAA ACACTAAAAG CGGGTGATCT AAGGACACGC
    GTCAGTCGCT GCCAAGTTTG TATGAAGAGG ACGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa14361
    Clone ID Related Accession (Same CDS sequence) XM_002931817.4
    Accession Version XM_002931817.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5067bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product collagen alpha-5(IV) chain isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002931817.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAGACTGG GAAGTTGGCA GCGGATGCCC TGCCTGGCTG TACTGGTGTT GGTGCTGGGG 
    CACTGGGCAC AGAATGCAGA GTCTGCGGCC TGTCAAGGTT GTACATCGGT ATCGAAATGT
    GACTGCAGTG GTGTTAAAGG AGAAAAGGGT GAGAGAGGCC TTCCAGGGTT AGAAGGACAC
    CTTGGTCTAC CAGGATTTCC TGGACCTGAA GGACCGCCTG GACCTCGTGG TCCCAAGGGT
    GATTCTGGTT TATCAGGGTC TCCAGGACCC AAAGGAATAC GAGGACCTCC TGGACCACCC
    GGATTTCCAG GAAACCCTGG CCAGCCTGGC CTGCCGGGAC ATGATGGTGA ACCAGGGCCT
    GCAGGTATCC CAGGATGCAA TGGCACAAAG GGAGATCGTG GTTTCCCAGG AGGTCCAGGT
    TTTTCGGGTA CACCAGGAAT ACCAGGTCCC CCAGGATTAC CAGGTGTGAA AGGAGATCCA
    GGTGAATCTT TCCCTTTAGC ACCGGGACAA AAAGGTGATC CAGGATTACC AGGTCTTCCA
    GGTTTACCAG GTCGGACTGG TCCTATTGGA GTTCAAGGTC CTGTGGGTCG ACCTGGTCCT
    GCTGGCTTAC CGGGGTCACC AGGAGTACCT GGACCTCCAG GACCCAAGGG TAACATGGGG
    CTGAATTTCC AGGGATCAAA GGGAGAAAAG GGTGAACCTG GTATTCAAGG ACTACCAGGA
    CCACCGGGAC AACTCGAGCA AGTTGAACCT CCACAAACTA CAATTCAGAA GGGAGAGAAG
    GGTATCCCCG GTGACCAAGG ATTTCCAGGA GAGCGTGGGC CAATGGGTCC TCCTGGTCCT
    CCTGGAGGTG CAAAGGGAGA GAAGGGAGAA CCAGGGGAAC CTGGAAAGAT TGGGAAGCCT
    GGAAAAGATG GAAAGATTGG TCTGCCCGGT GGAGAGGGTT TCAGGGGTGA CCCAGGCTTG
    CCGGGAGGTC CAGGCAGAGA TGGGGACAAG GGTCAAAAAG GTGAAAATGG GCTCCCTGGG
    CCACCAGGAG AGGTAATTTC AAGACCAGGG ACAGGCAGAG TTGGACCAAA AGGAAACACA
    GGACCCCCTG GAATAACAGG GCCAAAAGGA GACCGTGGAT TCCCTGGATT ACAAGGTCCC
    CCTGGTTTTC CTGGAACACC AGGTACTTCA AGTGTAGGCC CATCAGGAAA TCCAGGCCTT
    CCTGGTGAAA GAGGGCAAAA AGGCGATGAT GGTCCACCAG GAATCTCTAT TCCAGGGCTT
    CCAGGTTCTG AAGGTCTTGC AGGACCCCCT GGTCCCCCAG GTTTGCCTGG TCGACCTGGA
    ACATCTGCAG TTGGTCCTGC AATGGTATGC CAGCCAGGGC TACCTGGTAC TCAAGGACTT
    CCAGGAGATC CAGGATTTTT AGGAGAAAGA GGACAGAAAG GTGACAAAGG AGACACATGT
    TATAACTGCA TAGGAAATGG TATTTTAGGT CCGAAGGGGG ATCCTGGTTT GCCTGGTTTC
    CCTGGAAGTC CAGGTTCACC TGGAGCTCCA GGGCAAAAAG GAGCAATGGG TATCCCAGGA
    ACAAATGGGC TTCCAGGACC TCCGGGTTTT CCTGGCAATC CTGGTATCTC TGGCCGACCC
    GGTCCCAAAG GAGAACCTGG AGAAGTAATT GGCGAAATTA AAGGAGAAAA GGGGGACCCT
    GGATTCCCAG GTCCAGCTGG ATCTCCTGGT CTGGATGGTG TCCCTGGAAG AGACGGAACC
    CCAGGGATCC CTGGCCCTAA GGGAGAGCCG GGTGGCTTCG CGTTGAAAGG CGATCGTGGT
    TTTCCTGGTG ACATTGGGTT CCCTGGACAT CCTGGAGATC GAGGACTAAT GGGTCCTCCT
    GGTTTTGGTC CCAGTGGTCC ACCTGGAGAG AAGGGAATTC AAGGTGTTTC TGGTCGTCCA
    GGAGCCCCTG GAATACCTGG CCCTAAAGGA TCTCCTGGAC AGACTGTGAT GGAAAAAGGG
    GATTTTGGAC CCCCTGGTCT CCCAGGTATC CCAGGGGATG CCGGAACTCC AGGTGAACCA
    GGTCTACCTG GTCAGCCAGG AATTCCAGGT GTTCCAGGTT CAAAAGGAGA ACCAGGCATT
    CCAGGCATTG GACTTCCTGG GTCTGCAGGC GCAAAAGGAT TTCCTGGTAA TACTGGGCCA
    CCAGGTGCAC CAGGGTTGGC AGGAAGACCT GGTTCAGATG GGTCACCAGG ACAGCCTGGC
    TTGCCAGGAC CAAAGGGTGA CCCTGGGTTT GGTTTACCTG GGCTACCAGG CTCCCCAGGC
    CCTGCTGGAT TGAAAGGATC CCAAGGACCA AAAGGAGAAT CAGGCTTCCC AGGTTCTCCA
    GGCCTTCCTG GACGAGCAGG ATTTGATGGG CCTGTGGGAC CAAAAGGTGA CTCTGGACAA
    AATGGCTTAC CAGGAACCCC TGGACCACCT GGGGTACCTG GTGCTGGTGG TCAAGGCCCT
    CCAGGACAAC CAGGGCTTCC AGGGCCAGCA GGCCCTCCAG GGAACACTGG AATACAGGGA
    GAGAAAGGAG ATCCTGGTGC TCCAGGGTTT GACATGCCAG GCCCACCAGG AGATCGTGGT
    AGCCCTGGAT TTCCAGGAGA AAGAGGGTTT ACAGGACCAC CAGGGCCACC TGGAGCCCAA
    GGTCGCGATG GATTACCAGG CTTCTCAGGT GCTAAAGGTG AAATGGGTGT AATGGGACCA
    CCAGGATCTG TCGGACCAGC TGGTAATCCT GGAAACCCTG GTTTACCTGG GTCTAAAGGA
    AATGATGGGA ATCCTGGACA ACCTGGCCTG TCTGGTTTAC CAGGGGCTGA AGGTGAAAAG
    GGAGAGAGAG GATTACCAGG ACCCCCTGGA AGAGTTGATT CTGCAAACCT ACAGTTTAAA
    GGAGTTAAAG GAGAACCTGG CTTGCCAGGT TCATCAGGAC TAAGTGGGGC AAAAGGTGCT
    GCTGGACTTC CTGGTAACTC AGGATCCCCA GGGCAGATTG GAATTCCAGG ATCTCCAGGT
    CTTCCAGGGC TCAAGGGAGA TTCTGGCCTT CCTGGTCAGC CGGGTACTTC AGGTCCTCCT
    GGATTAAAAG GGAGCATGGG AGAGATGGGA CTTCCTGGGC CTCCTGGTTC TAAAGGTTCC
    CCTGGACAGC CAGGAAGGCC TGGTTCAGCA GGGTCTCCTG GGTTTTCTGG ACTGAAAGGT
    GAAAAAGGTG AACCAGGAAT TTCAGGAGCT GGTATCCCAG GCTTTCCTGG GCAGAAGGGT
    GAACCAGGGC AAAATGGCTT TCCAGGCAGC CCTGGTAGTA AAGGCAACCC AGGAAACCCA
    GGACTGCCCG GCCTACCAGG GATTTCAGGA CCTAAAGGGG ATCCAGGACT TCCAGGATTC
    CCAGGCAGCC CTGGATCTCC AGGCCTTAAA GGAGTTGATG GGCCCACAGG AAACTCTGGG
    TTACCCGGAT CTCCAGGCTT ACGGGGTGAA TCAGGTCGTC CCGGACCTCA AGGAACTCCT
    GGTGAGAAGG GTCAACCAGG TCAGGATGGA ATTCCTGGGC CTTCTGGTTT GAAGGGAGAA
    CCAGGTTTGC CAGGTTTTGG ATCCCCAGGG CTGCCTGGCC TTCCAGGAAG TCAAGGTCAA
    AAGGGTGATG CAGGACTTTC TGGAAGCCCT GGAAACCCCG GACTTCCAGG GTTCAAAGGA
    GATCAAGGCT TTCCAGGATC TCCTGGGCCA CAAGGACCAA CAGGACAACC TGGCCTACCA
    GGGCCACGTA CAGAAGGACC CAAGGGAGAC CCTGGGCCTG TAGGTCCCCC TGGAAGACCA
    GGCCCTCAAG GTTATCCAGG TCCTCCTGGC TCAGAAGGTC CACGTGGTCC CCCTGGTGAT
    GGAGGCTTTA AAGGAGAAAA AGGAAACCCT GGATTTCCAG GTCAGCCTGG ACTTACAGGA
    TCTAAAGGAG AACATGGAGC ATCTGGTTTC CCAGGTGACC CAGGACGACC AGGTCTTAAT
    GGGATGAAAG GTGATCAAGG TGTTCCTGGT GTTCCAGGAT TCCCAGGTAT GAAAGGACCA
    GGTGGCCCTC CTGGATTGGC TGGCCTTAAA GGTGAATCCG GACTCCCAGG TGCTATAGGT
    TTGCCAGGTC TTCCTGGGCC TAAAGGAGAA TACCGTGAGA TAAAAGGAGC TCCAGGTCTG
    CCAGGTCCTC CAGGTCGCCC AGGAGTAAAT GGACCTCCAG GACCCCCCGG ATCACCAGGA
    CTTCCAGGTT CTTTGGGTCT CCCTGGTGAT ACTGGCAGAG ATGGACTCCC TGGATTTGAG
    GGACAACTTG GACGAAAGGG TGACCGAGGC CTACCAGGCC AACCAGGATC TCGTGGAATT
    AATGGCCCAC CTGGCCCAGA TGGTTTACAA GGCCCCCCTG GCCCCCCTGG TCTAGGATCT
    GTTGCTCATG GATTCCTTAT AACCCGACAC AGTCAGTCAA CAGATGCCCC TGGCTGTCCT
    TTTGGAACCA CCAGGATCTA TGATGGCTAT TCACTACTTT ATGTGCAAGG AAATGAGAGG
    GCACACGGAC AAGATTTGGG TACCGCTGGC AGCTGCCTCC GTCGCTTCAG CACAATGCCT
    TTCATGTTCT GCAACATTAA TAACGTGTGT AACTTTGCTT CAAGAAATGA CTACTCCTAC
    TGGTTGTCCA CGCCTGAGCC TATGCCTATG AGCATGGAAC CTTTAAGAGG GAGGGATATC
    CAACCATTCA TTAGCAGGTG TGCGGTCTGT GAAGCGCCAG CCATGGTGAT TGCAGTTCAC
    AGCCAGACAA TACAAATCCC ACAGTGCCCA CAAGGCTGGT CCTCTCTCTG GATTGGATAC
    TCTTTCATGA TGCATACAAG TGCCGGTGCT GAAGGCTCAG GACAAGCTCT GGCATCGCCT
    GGATCGTGCT TGGAGGAATT CCGATCTGCA CCTTTCATTG AATGTCATGG CCGGGGAACC
    TGCAATTACT ATGCCAATTC ATACAGTTTT TGGTTGGCCA CAGTGGAAAT GTCTGAAATG
    TTTAGCAAAC CTCAGTCAGA AACACTAAAA GCGGGTGATC TAAGGACACG CGTCAGTCGC
    TGCCAAGTTT GTATGAAGAG GACGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002931863.2
    CDS289..5355
    Translation

    Target ORF information:

    RefSeq Version XM_002931817.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis collagen, type IV, alpha 5 (col4a5), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002931817.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAGACTGG GAAGTTGGCA GCGGATGCCC TGCCTGGCTG TACTGGTGTT GGTGCTGGGG 
    CACTGGGCAC AGAATGCAGA GTCTGCGGCC TGTCAAGGTT GTACATCGGT ATCGAAATGT
    GACTGCAGTG GTGTTAAAGG AGAAAAGGGT GAGAGAGGCC TTCCAGGGTT AGAAGGACAC
    CTTGGTCTAC CAGGATTTCC TGGACCTGAA GGACCGCCTG GACCTCGTGG TCCCAAGGGT
    GATTCTGGTT TATCAGGGTC TCCAGGACCC AAAGGAATAC GAGGACCTCC TGGACCACCC
    GGATTTCCAG GAAACCCTGG CCAGCCTGGC CTGCCGGGAC ATGATGGTGA ACCAGGGCCT
    GCAGGTATCC CAGGATGCAA TGGCACAAAG GGAGATCGTG GTTTCCCAGG AGGTCCAGGT
    TTTTCGGGTA CACCAGGAAT ACCAGGTCCC CCAGGATTAC CAGGTGTGAA AGGAGATCCA
    GGTGAATCTT TCCCTTTAGC ACCGGGACAA AAAGGTGATC CAGGATTACC AGGTCTTCCA
    GGTTTACCAG GTCGGACTGG TCCTATTGGA GTTCAAGGTC CTGTGGGTCG ACCTGGTCCT
    GCTGGCTTAC CGGGGTCACC AGGAGTACCT GGACCTCCAG GACCCAAGGG TAACATGGGG
    CTGAATTTCC AGGGATCAAA GGGAGAAAAG GGTGAACCTG GTATTCAAGG ACTACCAGGA
    CCACCGGGAC AACTCGAGCA AGTTGAACCT CCACAAACTA CAATTCAGAA GGGAGAGAAG
    GGTATCCCCG GTGACCAAGG ATTTCCAGGA GAGCGTGGGC CAATGGGTCC TCCTGGTCCT
    CCTGGAGGTG CAAAGGGAGA GAAGGGAGAA CCAGGGGAAC CTGGAAAGAT TGGGAAGCCT
    GGAAAAGATG GAAAGATTGG TCTGCCCGGT GGAGAGGGTT TCAGGGGTGA CCCAGGCTTG
    CCGGGAGGTC CAGGCAGAGA TGGGGACAAG GGTCAAAAAG GTGAAAATGG GCTCCCTGGG
    CCACCAGGAG AGGTAATTTC AAGACCAGGG ACAGGCAGAG TTGGACCAAA AGGAAACACA
    GGACCCCCTG GAATAACAGG GCCAAAAGGA GACCGTGGAT TCCCTGGATT ACAAGGTCCC
    CCTGGTTTTC CTGGAACACC AGGTACTTCA AGTGTAGGCC CATCAGGAAA TCCAGGCCTT
    CCTGGTGAAA GAGGGCAAAA AGGCGATGAT GGTCCACCAG GAATCTCTAT TCCAGGGCTT
    CCAGGTTCTG AAGGTCTTGC AGGACCCCCT GGTCCCCCAG GTTTGCCTGG TCGACCTGGA
    ACATCTGCAG TTGGTCCTGC AATGGTATGC CAGCCAGGGC TACCTGGTAC TCAAGGACTT
    CCAGGAGATC CAGGATTTTT AGGAGAAAGA GGACAGAAAG GTGACAAAGG AGACACATGT
    TATAACTGCA TAGGAAATGG TATTTTAGGT CCGAAGGGGG ATCCTGGTTT GCCTGGTTTC
    CCTGGAAGTC CAGGTTCACC TGGAGCTCCA GGGCAAAAAG GAGCAATGGG TATCCCAGGA
    ACAAATGGGC TTCCAGGACC TCCGGGTTTT CCTGGCAATC CTGGTATCTC TGGCCGACCC
    GGTCCCAAAG GAGAACCTGG AGAAGTAATT GGCGAAATTA AAGGAGAAAA GGGGGACCCT
    GGATTCCCAG GTCCAGCTGG ATCTCCTGGT CTGGATGGTG TCCCTGGAAG AGACGGAACC
    CCAGGGATCC CTGGCCCTAA GGGAGAGCCG GGTGGCTTCG CGTTGAAAGG CGATCGTGGT
    TTTCCTGGTG ACATTGGGTT CCCTGGACAT CCTGGAGATC GAGGACTAAT GGGTCCTCCT
    GGTTTTGGTC CCAGTGGTCC ACCTGGAGAG AAGGGAATTC AAGGTGTTTC TGGTCGTCCA
    GGAGCCCCTG GAATACCTGG CCCTAAAGGA TCTCCTGGAC AGACTGTGAT GGAAAAAGGG
    GATTTTGGAC CCCCTGGTCT CCCAGGTATC CCAGGGGATG CCGGAACTCC AGGTGAACCA
    GGTCTACCTG GTCAGCCAGG AATTCCAGGT GTTCCAGGTT CAAAAGGAGA ACCAGGCATT
    CCAGGCATTG GACTTCCTGG GTCTGCAGGC GCAAAAGGAT TTCCTGGTAA TACTGGGCCA
    CCAGGTGCAC CAGGGTTGGC AGGAAGACCT GGTTCAGATG GGTCACCAGG ACAGCCTGGC
    TTGCCAGGAC CAAAGGGTGA CCCTGGGTTT GGTTTACCTG GGCTACCAGG CTCCCCAGGC
    CCTGCTGGAT TGAAAGGATC CCAAGGACCA AAAGGAGAAT CAGGCTTCCC AGGTTCTCCA
    GGCCTTCCTG GACGAGCAGG ATTTGATGGG CCTGTGGGAC CAAAAGGTGA CTCTGGACAA
    AATGGCTTAC CAGGAACCCC TGGACCACCT GGGGTACCTG GTGCTGGTGG TCAAGGCCCT
    CCAGGACAAC CAGGGCTTCC AGGGCCAGCA GGCCCTCCAG GGAACACTGG AATACAGGGA
    GAGAAAGGAG ATCCTGGTGC TCCAGGGTTT GACATGCCAG GCCCACCAGG AGATCGTGGT
    AGCCCTGGAT TTCCAGGAGA AAGAGGGTTT ACAGGACCAC CAGGGCCACC TGGAGCCCAA
    GGTCGCGATG GATTACCAGG CTTCTCAGGT GCTAAAGGTG AAATGGGTGT AATGGGACCA
    CCAGGATCTG TCGGACCAGC TGGTAATCCT GGAAACCCTG GTTTACCTGG GTCTAAAGGA
    AATGATGGGA ATCCTGGACA ACCTGGCCTG TCTGGTTTAC CAGGGGCTGA AGGTGAAAAG
    GGAGAGAGAG GATTACCAGG ACCCCCTGGA AGAGTTGATT CTGCAAACCT ACAGTTTAAA
    GGAGTTAAAG GAGAACCTGG CTTGCCAGGT TCATCAGGAC TAAGTGGGGC AAAAGGTGCT
    GCTGGACTTC CTGGTAACTC AGGATCCCCA GGGCAGATTG GAATTCCAGG ATCTCCAGGT
    CTTCCAGGGC TCAAGGGAGA TTCTGGCCTT CCTGGTCAGC CGGGTACTTC AGGTCCTCCT
    GGATTAAAAG GGAGCATGGG AGAGATGGGA CTTCCTGGGC CTCCTGGTTC TAAAGGTTCC
    CCTGGACAGC CAGGAAGGCC TGGTTCAGCA GGGTCTCCTG GGTTTTCTGG ACTGAAAGGT
    GAAAAAGGTG AACCAGGAAT TTCAGGAGCT GGTATCCCAG GCTTTCCTGG GCAGAAGGGT
    GAACCAGGGC AAAATGGCTT TCCAGGCAGC CCTGGTAGTA AAGGCAACCC AGGAAACCCA
    GGACTGCCCG GCCTACCAGG GATTTCAGGA CCTAAAGGGG ATCCAGGACT TCCAGGATTC
    CCAGGCAGCC CTGGATCTCC AGGCCTTAAA GGAGTTGATG GGCCCACAGG AAACTCTGGG
    TTACCCGGAT CTCCAGGCTT ACGGGGTGAA TCAGGTCGTC CCGGACCTCA AGGAACTCCT
    GGTGAGAAGG GTCAACCAGG TCAGGATGGA ATTCCTGGGC CTTCTGGTTT GAAGGGAGAA
    CCAGGTTTGC CAGGTTTTGG ATCCCCAGGG CTGCCTGGCC TTCCAGGAAG TCAAGGTCAA
    AAGGGTGATG CAGGACTTTC TGGAAGCCCT GGAAACCCCG GACTTCCAGG GTTCAAAGGA
    GATCAAGGCT TTCCAGGATC TCCTGGGCCA CAAGGACCAA CAGGACAACC TGGCCTACCA
    GGGCCACGTA CAGAAGGACC CAAGGGAGAC CCTGGGCCTG TAGGTCCCCC TGGAAGACCA
    GGCCCTCAAG GTTATCCAGG TCCTCCTGGC TCAGAAGGTC CACGTGGTCC CCCTGGTGAT
    GGAGGCTTTA AAGGAGAAAA AGGAAACCCT GGATTTCCAG GTCAGCCTGG ACTTACAGGA
    TCTAAAGGAG AACATGGAGC ATCTGGTTTC CCAGGTGACC CAGGACGACC AGGTCTTAAT
    GGGATGAAAG GTGATCAAGG TGTTCCTGGT GTTCCAGGAT TCCCAGGTAT GAAAGGACCA
    GGTGGCCCTC CTGGATTGGC TGGCCTTAAA GGTGAATCCG GACTCCCAGG TGCTATAGGT
    TTGCCAGGTC TTCCTGGGCC TAAAGGAGAA TACCGTGAGA TAAAAGGAGC TCCAGGTCTG
    CCAGGTCCTC CAGGTCGCCC AGGAGTAAAT GGACCTCCAG GACCCCCCGG ATCACCAGGA
    CTTCCAGGTT CTTTGGGTCT CCCTGGTGAT ACTGGCAGAG ATGGACTCCC TGGATTTGAG
    GGACAACTTG GACGAAAGGG TGACCGAGGC CTACCAGGCC AACCAGGATC TCGTGGAATT
    AATGGCCCAC CTGGCCCAGA TGGTTTACAA GGCCCCCCTG GCCCCCCTGG TCTAGGATCT
    GTTGCTCATG GATTCCTTAT AACCCGACAC AGTCAGTCAA CAGATGCCCC TGGCTGTCCT
    TTTGGAACCA CCAGGATCTA TGATGGCTAT TCACTACTTT ATGTGCAAGG AAATGAGAGG
    GCACACGGAC AAGATTTGGG TACCGCTGGC AGCTGCCTCC GTCGCTTCAG CACAATGCCT
    TTCATGTTCT GCAACATTAA TAACGTGTGT AACTTTGCTT CAAGAAATGA CTACTCCTAC
    TGGTTGTCCA CGCCTGAGCC TATGCCTATG AGCATGGAAC CTTTAAGAGG GAGGGATATC
    CAACCATTCA TTAGCAGGTG TGCGGTCTGT GAAGCGCCAG CCATGGTGAT TGCAGTTCAC
    AGCCAGACAA TACAAATCCC ACAGTGCCCA CAAGGCTGGT CCTCTCTCTG GATTGGATAC
    TCTTTCATGA TGCATACAAG TGCCGGTGCT GAAGGCTCAG GACAAGCTCT GGCATCGCCT
    GGATCGTGCT TGGAGGAATT CCGATCTGCA CCTTTCATTG AATGTCATGG CCGGGGAACC
    TGCAATTACT ATGCCAATTC ATACAGTTTT TGGTTGGCCA CAGTGGAAAT GTCTGAAATG
    TTTAGCAAAC CTCAGTCAGA AACACTAAAA GCGGGTGATC TAAGGACACG CGTCAGTCGC
    TGCCAAGTTT GTATGAAGAG GACGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa14362
    Clone ID Related Accession (Same CDS sequence) XM_004916867.3
    Accession Version XM_004916867.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5067bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product collagen alpha-5(IV) chain isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004916867.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAGACTGG GAAGTTGGCA GCGGATGCCC TGCCTGGCTG TACTGGTGTT GGTGCTGGGG 
    CACTGGGCAC AGAATGCAGA GTCTGCGGCC TGTCAAGGTT GTACATCGGT ATCGAAATGT
    GACTGCAGTG GTGTTAAAGG AGAAAAGGGT GAGAGAGGCC TTCCAGGGTT AGAAGGACAC
    CTTGGTCTAC CAGGATTTCC TGGACCTGAA GGACCGCCTG GACCTCGTGG TCCCAAGGGT
    GATTCTGGTT TATCAGGGTC TCCAGGACCC AAAGGAATAC GAGGACCTCC TGGACCACCC
    GGATTTCCAG GAAACCCTGG CCAGCCTGGC CTGCCGGGAC ATGATGGTGA ACCAGGGCCT
    GCAGGTATCC CAGGATGCAA TGGCACAAAG GGAGATCGTG GTTTCCCAGG AGGTCCAGGT
    TTTTCGGGTA CACCAGGAAT ACCAGGTCCC CCAGGATTAC CAGGTGTGAA AGGAGATCCA
    GGTGAATCTT TCCCTTTAGC ACCGGGACAA AAAGGTGATC CAGGATTACC AGGTCTTCCA
    GGTTTACCAG GTCGGACTGG TCCTATTGGA GTTCAAGGTC CTGTGGGTCG ACCTGGTCCT
    GCTGGCTTAC CGGGGTCACC AGGAGTACCT GGACCTCCAG GACCCAAGGG TAACATGGGG
    CTGAATTTCC AGGGATCAAA GGGAGAAAAG GGTGAACCTG GTATTCAAGG ACTACCAGGA
    CCACCGGGAC AACTCGAGCA AGTTGAACCT CCACAAACTA CAATTCAGAA GGGAGAGAAG
    GGTATCCCCG GTGACCAAGG ATTTCCAGGA GAGCGTGGGC CAATGGGTCC TCCTGGTCCT
    CCTGGAGGTG CAAAGGGAGA GAAGGGAGAA CCAGGGGAAC CTGGAAAGAT TGGGAAGCCT
    GGAAAAGATG GAAAGATTGG TCTGCCCGGT GGAGAGGGTT TCAGGGGTGA CCCAGGCTTG
    CCGGGAGGTC CAGGCAGAGA TGGGGACAAG GGTCAAAAAG GTGAAAATGG GCTCCCTGGG
    CCACCAGGAG AGGTAATTTC AAGACCAGGG ACAGGCAGAG TTGGACCAAA AGGAAACACA
    GGACCCCCTG GAATAACAGG GCCAAAAGGA GACCGTGGAT TCCCTGGATT ACAAGGTCCC
    CCTGGTTTTC CTGGAACACC AGGTACTTCA AGTGTAGGCC CATCAGGAAA TCCAGGCCTT
    CCTGGTGAAA GAGGGCAAAA AGGCGATGAT GGTCCACCAG GAATCTCTAT TCCAGGGCTT
    CCAGGTTCTG AAGGTCTTGC AGGACCCCCT GGTCCCCCAG GTTTGCCTGG TCGACCTGGA
    ACATCTGCAG TTGGTCCTGC AATGGTATGC CAGCCAGGGC TACCTGGTAC TCAAGGACTT
    CCAGGAGATC CAGGATTTTT AGGAGAAAGA GGACAGAAAG GTGACAAAGG AGACACATGT
    TATAACTGCA TAGGAAATGG TATTTTAGGT CCGAAGGGGG ATCCTGGTTT GCCTGGTTTC
    CCTGGAAGTC CAGGTTCACC TGGAGCTCCA GGGCAAAAAG GAGCAATGGG TATCCCAGGA
    ACAAATGGGC TTCCAGGACC TCCGGGTTTT CCTGGCAATC CTGGTATCTC TGGCCGACCC
    GGTCCCAAAG GAGAACCTGG AGAAGTAATT GGCGAAATTA AAGGAGAAAA GGGGGACCCT
    GGATTCCCAG GTCCAGCTGG ATCTCCTGGT CTGGATGGTG TCCCTGGAAG AGACGGAACC
    CCAGGGATCC CTGGCCCTAA GGGAGAGCCG GGTGGCTTCG CGTTGAAAGG CGATCGTGGT
    TTTCCTGGTG ACATTGGGTT CCCTGGACAT CCTGGAGATC GAGGACTAAT GGGTCCTCCT
    GGTTTTGGTC CCAGTGGTCC ACCTGGAGAG AAGGGAATTC AAGGTGTTTC TGGTCGTCCA
    GGAGCCCCTG GAATACCTGG CCCTAAAGGA TCTCCTGGAC AGACTGTGAT GGAAAAAGGG
    GATTTTGGAC CCCCTGGTCT CCCAGGTATC CCAGGGGATG CCGGAACTCC AGGTGAACCA
    GGTCTACCTG GTCAGCCAGG AATTCCAGGT GTTCCAGGTT CAAAAGGAGA ACCAGGCATT
    CCAGGCATTG GACTTCCTGG GTCTGCAGGC GCAAAAGGAT TTCCTGGTAA TACTGGGCCA
    CCAGGTGCAC CAGGGTTGGC AGGAAGACCT GGTTCAGATG GGTCACCAGG ACAGCCTGGC
    TTGCCAGGAC CAAAGGGTGA CCCTGGGTTT GGTTTACCTG GGCTACCAGG CTCCCCAGGC
    CCTGCTGGAT TGAAAGGATC CCAAGGACCA AAAGGAGAAT CAGGCTTCCC AGGTTCTCCA
    GGCCTTCCTG GACGAGCAGG ATTTGATGGG CCTGTGGGAC CAAAAGGTGA CTCTGGACAA
    AATGGCTTAC CAGGAACCCC TGGACCACCT GGGGTACCTG GTGCTGGTGG TCAAGGCCCT
    CCAGGACAAC CAGGGCTTCC AGGGCCAGCA GGCCCTCCAG GTCCCACAGG GAACACTGGA
    ATACAGGGAG AGAAAGGAGA TCCTGGTGCT CCAGGGTTTG ACATGCCAGG CCCACCAGGA
    GATCGTGGTA GCCCTGGATT TCCAGGAGAA AGAGGGTTTA CAGGACCACC AGGGCCACCT
    GGAGCCCAAG GTCGCGATGG ATTACCAGGC TTCTCAGGTG CTAAAGGTGA AATGGGTGTA
    ATGGGACCAC CAGGATCTGT CGGACCAGCT GGTAATCCTG GAAACCCTGG TTTACCTGGG
    TCTAAAGGAA ATGATGGGAA TCCTGGACAA CCTGGCCTGT CTGGTTTACC AGGGGCTGAA
    GGTGAAAAGG GAGAGAGAGG ATTACCAGGA CCCCCTGGAA GAGTTGATTC TGCAAACCTA
    CAGTTTAAAG GAGTTAAAGG AGAACCTGGC TTGCCAGGTT CATCAGGACT AAGTGGGGCA
    AAAGGTGCTG CTGGACTTCC TGGTAACTCA GGATCCCCAG GGCAGATTGG AATTCCAGGA
    TCTCCAGGTC TTCCAGGGCT CAAGGGAGAT TCTGGCCTTC CTGGTCAGCC GGGTACTTCA
    GGTCCTCCTG GATTAAAAGG GAGCATGGGA GAGATGGGAC TTCCTGGGCC TCCTGGTTCT
    AAAGGTTCCC CTGGACAGCC AGGAAGGCCT GGTTCAGCAG GGTCTCCTGG GTTTTCTGGA
    CTGAAAGGTG AAAAAGGTGA ACCAGGAATT TCAGGAGCTG GTATCCCAGG CTTTCCTGGG
    CAGAAGGGTG AACCAGGGCA AAATGGCTTT CCAGGCAGCC CTGGTAGTAA AGGCAACCCA
    GGAAACCCAG GACTGCCCGG CCTACCAGGG ATTTCAGGAC CTAAAGGGGA TCCAGGACTT
    CCAGGATTCC CAGGCAGCCC TGGATCTCCA GGCCTTAAAG GAGTTGATGG GCCCACAGGA
    AACTCTGGGT TACCCGGATC TCCAGGCTTA CGGGGTGAAT CAGGTCGTCC CGGACCTCAA
    GGAACTCCTG GTGAGAAGGG TCAACCAGGT CAGGATGGAA TTCCTGGGCC TTCTGGTTTG
    AAGGGAGAAC CAGGTTTGCC AGGTTTTGGA TCCCCAGGGC TGCCTGGCCT TCCAGGAAGT
    CAAGGTCAAA AGGGTGATGC AGGACTTTCT GGAAGCCCTG GAAACCCCGG ACTTCCAGGG
    TTCAAAGGAG ATCAAGGCTT TCCAGGATCT CCTGGGCCAC AAGGACCAAC AGGACAACCT
    GGCCTACCAG GGCCACGTAC AGAAGGACCC AAGGGAGACC CTGGGCCTGT AGGTCCCCCT
    GGAAGACCAG GTTATCCAGG TCCTCCTGGC TCAGAAGGTC CACGTGGTCC CCCTGGTGAT
    GGAGGCTTTA AAGGAGAAAA AGGAAACCCT GGATTTCCAG GTCAGCCTGG ACTTACAGGA
    TCTAAAGGAG AACATGGAGC ATCTGGTTTC CCAGGTGACC CAGGACGACC AGGTCTTAAT
    GGGATGAAAG GTGATCAAGG TGTTCCTGGT GTTCCAGGAT TCCCAGGTAT GAAAGGACCA
    GGTGGCCCTC CTGGATTGGC TGGCCTTAAA GGTGAATCCG GACTCCCAGG TGCTATAGGT
    TTGCCAGGTC TTCCTGGGCC TAAAGGAGAA TACCGTGAGA TAAAAGGAGC TCCAGGTCTG
    CCAGGTCCTC CAGGTCGCCC AGGAGTAAAT GGACCTCCAG GACCCCCCGG ATCACCAGGA
    CTTCCAGGTT CTTTGGGTCT CCCTGGTGAT ACTGGCAGAG ATGGACTCCC TGGATTTGAG
    GGACAACTTG GACGAAAGGG TGACCGAGGC CTACCAGGCC AACCAGGATC TCGTGGAATT
    AATGGCCCAC CTGGCCCAGA TGGTTTACAA GGCCCCCCTG GCCCCCCTGG TCTAGGATCT
    GTTGCTCATG GATTCCTTAT AACCCGACAC AGTCAGTCAA CAGATGCCCC TGGCTGTCCT
    TTTGGAACCA CCAGGATCTA TGATGGCTAT TCACTACTTT ATGTGCAAGG AAATGAGAGG
    GCACACGGAC AAGATTTGGG TACCGCTGGC AGCTGCCTCC GTCGCTTCAG CACAATGCCT
    TTCATGTTCT GCAACATTAA TAACGTGTGT AACTTTGCTT CAAGAAATGA CTACTCCTAC
    TGGTTGTCCA CGCCTGAGCC TATGCCTATG AGCATGGAAC CTTTAAGAGG GAGGGATATC
    CAACCATTCA TTAGCAGGTG TGCGGTCTGT GAAGCGCCAG CCATGGTGAT TGCAGTTCAC
    AGCCAGACAA TACAAATCCC ACAGTGCCCA CAAGGCTGGT CCTCTCTCTG GATTGGATAC
    TCTTTCATGA TGCATACAAG TGCCGGTGCT GAAGGCTCAG GACAAGCTCT GGCATCGCCT
    GGATCGTGCT TGGAGGAATT CCGATCTGCA CCTTTCATTG AATGTCATGG CCGGGGAACC
    TGCAATTACT ATGCCAATTC ATACAGTTTT TGGTTGGCCA CAGTGGAAAT GTCTGAAATG
    TTTAGCAAAC CTCAGTCAGA AACACTAAAA GCGGGTGATC TAAGGACACG CGTCAGTCGC
    TGCCAAGTTT GTATGAAGAG GACGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004916924.1
    CDS289..5355
    Translation

    Target ORF information:

    RefSeq Version XM_004916867.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis collagen, type IV, alpha 5 (col4a5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004916867.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAGACTGG GAAGTTGGCA GCGGATGCCC TGCCTGGCTG TACTGGTGTT GGTGCTGGGG 
    CACTGGGCAC AGAATGCAGA GTCTGCGGCC TGTCAAGGTT GTACATCGGT ATCGAAATGT
    GACTGCAGTG GTGTTAAAGG AGAAAAGGGT GAGAGAGGCC TTCCAGGGTT AGAAGGACAC
    CTTGGTCTAC CAGGATTTCC TGGACCTGAA GGACCGCCTG GACCTCGTGG TCCCAAGGGT
    GATTCTGGTT TATCAGGGTC TCCAGGACCC AAAGGAATAC GAGGACCTCC TGGACCACCC
    GGATTTCCAG GAAACCCTGG CCAGCCTGGC CTGCCGGGAC ATGATGGTGA ACCAGGGCCT
    GCAGGTATCC CAGGATGCAA TGGCACAAAG GGAGATCGTG GTTTCCCAGG AGGTCCAGGT
    TTTTCGGGTA CACCAGGAAT ACCAGGTCCC CCAGGATTAC CAGGTGTGAA AGGAGATCCA
    GGTGAATCTT TCCCTTTAGC ACCGGGACAA AAAGGTGATC CAGGATTACC AGGTCTTCCA
    GGTTTACCAG GTCGGACTGG TCCTATTGGA GTTCAAGGTC CTGTGGGTCG ACCTGGTCCT
    GCTGGCTTAC CGGGGTCACC AGGAGTACCT GGACCTCCAG GACCCAAGGG TAACATGGGG
    CTGAATTTCC AGGGATCAAA GGGAGAAAAG GGTGAACCTG GTATTCAAGG ACTACCAGGA
    CCACCGGGAC AACTCGAGCA AGTTGAACCT CCACAAACTA CAATTCAGAA GGGAGAGAAG
    GGTATCCCCG GTGACCAAGG ATTTCCAGGA GAGCGTGGGC CAATGGGTCC TCCTGGTCCT
    CCTGGAGGTG CAAAGGGAGA GAAGGGAGAA CCAGGGGAAC CTGGAAAGAT TGGGAAGCCT
    GGAAAAGATG GAAAGATTGG TCTGCCCGGT GGAGAGGGTT TCAGGGGTGA CCCAGGCTTG
    CCGGGAGGTC CAGGCAGAGA TGGGGACAAG GGTCAAAAAG GTGAAAATGG GCTCCCTGGG
    CCACCAGGAG AGGTAATTTC AAGACCAGGG ACAGGCAGAG TTGGACCAAA AGGAAACACA
    GGACCCCCTG GAATAACAGG GCCAAAAGGA GACCGTGGAT TCCCTGGATT ACAAGGTCCC
    CCTGGTTTTC CTGGAACACC AGGTACTTCA AGTGTAGGCC CATCAGGAAA TCCAGGCCTT
    CCTGGTGAAA GAGGGCAAAA AGGCGATGAT GGTCCACCAG GAATCTCTAT TCCAGGGCTT
    CCAGGTTCTG AAGGTCTTGC AGGACCCCCT GGTCCCCCAG GTTTGCCTGG TCGACCTGGA
    ACATCTGCAG TTGGTCCTGC AATGGTATGC CAGCCAGGGC TACCTGGTAC TCAAGGACTT
    CCAGGAGATC CAGGATTTTT AGGAGAAAGA GGACAGAAAG GTGACAAAGG AGACACATGT
    TATAACTGCA TAGGAAATGG TATTTTAGGT CCGAAGGGGG ATCCTGGTTT GCCTGGTTTC
    CCTGGAAGTC CAGGTTCACC TGGAGCTCCA GGGCAAAAAG GAGCAATGGG TATCCCAGGA
    ACAAATGGGC TTCCAGGACC TCCGGGTTTT CCTGGCAATC CTGGTATCTC TGGCCGACCC
    GGTCCCAAAG GAGAACCTGG AGAAGTAATT GGCGAAATTA AAGGAGAAAA GGGGGACCCT
    GGATTCCCAG GTCCAGCTGG ATCTCCTGGT CTGGATGGTG TCCCTGGAAG AGACGGAACC
    CCAGGGATCC CTGGCCCTAA GGGAGAGCCG GGTGGCTTCG CGTTGAAAGG CGATCGTGGT
    TTTCCTGGTG ACATTGGGTT CCCTGGACAT CCTGGAGATC GAGGACTAAT GGGTCCTCCT
    GGTTTTGGTC CCAGTGGTCC ACCTGGAGAG AAGGGAATTC AAGGTGTTTC TGGTCGTCCA
    GGAGCCCCTG GAATACCTGG CCCTAAAGGA TCTCCTGGAC AGACTGTGAT GGAAAAAGGG
    GATTTTGGAC CCCCTGGTCT CCCAGGTATC CCAGGGGATG CCGGAACTCC AGGTGAACCA
    GGTCTACCTG GTCAGCCAGG AATTCCAGGT GTTCCAGGTT CAAAAGGAGA ACCAGGCATT
    CCAGGCATTG GACTTCCTGG GTCTGCAGGC GCAAAAGGAT TTCCTGGTAA TACTGGGCCA
    CCAGGTGCAC CAGGGTTGGC AGGAAGACCT GGTTCAGATG GGTCACCAGG ACAGCCTGGC
    TTGCCAGGAC CAAAGGGTGA CCCTGGGTTT GGTTTACCTG GGCTACCAGG CTCCCCAGGC
    CCTGCTGGAT TGAAAGGATC CCAAGGACCA AAAGGAGAAT CAGGCTTCCC AGGTTCTCCA
    GGCCTTCCTG GACGAGCAGG ATTTGATGGG CCTGTGGGAC CAAAAGGTGA CTCTGGACAA
    AATGGCTTAC CAGGAACCCC TGGACCACCT GGGGTACCTG GTGCTGGTGG TCAAGGCCCT
    CCAGGACAAC CAGGGCTTCC AGGGCCAGCA GGCCCTCCAG GTCCCACAGG GAACACTGGA
    ATACAGGGAG AGAAAGGAGA TCCTGGTGCT CCAGGGTTTG ACATGCCAGG CCCACCAGGA
    GATCGTGGTA GCCCTGGATT TCCAGGAGAA AGAGGGTTTA CAGGACCACC AGGGCCACCT
    GGAGCCCAAG GTCGCGATGG ATTACCAGGC TTCTCAGGTG CTAAAGGTGA AATGGGTGTA
    ATGGGACCAC CAGGATCTGT CGGACCAGCT GGTAATCCTG GAAACCCTGG TTTACCTGGG
    TCTAAAGGAA ATGATGGGAA TCCTGGACAA CCTGGCCTGT CTGGTTTACC AGGGGCTGAA
    GGTGAAAAGG GAGAGAGAGG ATTACCAGGA CCCCCTGGAA GAGTTGATTC TGCAAACCTA
    CAGTTTAAAG GAGTTAAAGG AGAACCTGGC TTGCCAGGTT CATCAGGACT AAGTGGGGCA
    AAAGGTGCTG CTGGACTTCC TGGTAACTCA GGATCCCCAG GGCAGATTGG AATTCCAGGA
    TCTCCAGGTC TTCCAGGGCT CAAGGGAGAT TCTGGCCTTC CTGGTCAGCC GGGTACTTCA
    GGTCCTCCTG GATTAAAAGG GAGCATGGGA GAGATGGGAC TTCCTGGGCC TCCTGGTTCT
    AAAGGTTCCC CTGGACAGCC AGGAAGGCCT GGTTCAGCAG GGTCTCCTGG GTTTTCTGGA
    CTGAAAGGTG AAAAAGGTGA ACCAGGAATT TCAGGAGCTG GTATCCCAGG CTTTCCTGGG
    CAGAAGGGTG AACCAGGGCA AAATGGCTTT CCAGGCAGCC CTGGTAGTAA AGGCAACCCA
    GGAAACCCAG GACTGCCCGG CCTACCAGGG ATTTCAGGAC CTAAAGGGGA TCCAGGACTT
    CCAGGATTCC CAGGCAGCCC TGGATCTCCA GGCCTTAAAG GAGTTGATGG GCCCACAGGA
    AACTCTGGGT TACCCGGATC TCCAGGCTTA CGGGGTGAAT CAGGTCGTCC CGGACCTCAA
    GGAACTCCTG GTGAGAAGGG TCAACCAGGT CAGGATGGAA TTCCTGGGCC TTCTGGTTTG
    AAGGGAGAAC CAGGTTTGCC AGGTTTTGGA TCCCCAGGGC TGCCTGGCCT TCCAGGAAGT
    CAAGGTCAAA AGGGTGATGC AGGACTTTCT GGAAGCCCTG GAAACCCCGG ACTTCCAGGG
    TTCAAAGGAG ATCAAGGCTT TCCAGGATCT CCTGGGCCAC AAGGACCAAC AGGACAACCT
    GGCCTACCAG GGCCACGTAC AGAAGGACCC AAGGGAGACC CTGGGCCTGT AGGTCCCCCT
    GGAAGACCAG GTTATCCAGG TCCTCCTGGC TCAGAAGGTC CACGTGGTCC CCCTGGTGAT
    GGAGGCTTTA AAGGAGAAAA AGGAAACCCT GGATTTCCAG GTCAGCCTGG ACTTACAGGA
    TCTAAAGGAG AACATGGAGC ATCTGGTTTC CCAGGTGACC CAGGACGACC AGGTCTTAAT
    GGGATGAAAG GTGATCAAGG TGTTCCTGGT GTTCCAGGAT TCCCAGGTAT GAAAGGACCA
    GGTGGCCCTC CTGGATTGGC TGGCCTTAAA GGTGAATCCG GACTCCCAGG TGCTATAGGT
    TTGCCAGGTC TTCCTGGGCC TAAAGGAGAA TACCGTGAGA TAAAAGGAGC TCCAGGTCTG
    CCAGGTCCTC CAGGTCGCCC AGGAGTAAAT GGACCTCCAG GACCCCCCGG ATCACCAGGA
    CTTCCAGGTT CTTTGGGTCT CCCTGGTGAT ACTGGCAGAG ATGGACTCCC TGGATTTGAG
    GGACAACTTG GACGAAAGGG TGACCGAGGC CTACCAGGCC AACCAGGATC TCGTGGAATT
    AATGGCCCAC CTGGCCCAGA TGGTTTACAA GGCCCCCCTG GCCCCCCTGG TCTAGGATCT
    GTTGCTCATG GATTCCTTAT AACCCGACAC AGTCAGTCAA CAGATGCCCC TGGCTGTCCT
    TTTGGAACCA CCAGGATCTA TGATGGCTAT TCACTACTTT ATGTGCAAGG AAATGAGAGG
    GCACACGGAC AAGATTTGGG TACCGCTGGC AGCTGCCTCC GTCGCTTCAG CACAATGCCT
    TTCATGTTCT GCAACATTAA TAACGTGTGT AACTTTGCTT CAAGAAATGA CTACTCCTAC
    TGGTTGTCCA CGCCTGAGCC TATGCCTATG AGCATGGAAC CTTTAAGAGG GAGGGATATC
    CAACCATTCA TTAGCAGGTG TGCGGTCTGT GAAGCGCCAG CCATGGTGAT TGCAGTTCAC
    AGCCAGACAA TACAAATCCC ACAGTGCCCA CAAGGCTGGT CCTCTCTCTG GATTGGATAC
    TCTTTCATGA TGCATACAAG TGCCGGTGCT GAAGGCTCAG GACAAGCTCT GGCATCGCCT
    GGATCGTGCT TGGAGGAATT CCGATCTGCA CCTTTCATTG AATGTCATGG CCGGGGAACC
    TGCAATTACT ATGCCAATTC ATACAGTTTT TGGTTGGCCA CAGTGGAAAT GTCTGAAATG
    TTTAGCAAAC CTCAGTCAGA AACACTAAAA GCGGGTGATC TAAGGACACG CGTCAGTCGC
    TGCCAAGTTT GTATGAAGAG GACGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa14363
    Clone ID Related Accession (Same CDS sequence) XM_004916868.3
    Accession Version XM_004916868.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5058bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product collagen alpha-5(IV) chain isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004916868.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAGACTGG GAAGTTGGCA GCGGATGCCC TGCCTGGCTG TACTGGTGTT GGTGCTGGGG 
    CACTGGGCAC AGAATGCAGA GTCTGCGGCC TGTCAAGGTT GTACATCGGT ATCGAAATGT
    GACTGCAGTG GTGTTAAAGG AGAAAAGGGT GAGAGAGGCC TTCCAGGGTT AGAAGGACAC
    CTTGGTCTAC CAGGATTTCC TGGACCTGAA GGACCGCCTG GACCTCGTGG TCCCAAGGGT
    GATTCTGGTT TATCAGGGTC TCCAGGACCC AAAGGAATAC GAGGACCTCC TGGACCACCC
    GGATTTCCAG GAAACCCTGG CCAGCCTGGC CTGCCGGGAC ATGATGGTGA ACCAGGGCCT
    GCAGGTATCC CAGGATGCAA TGGCACAAAG GGAGATCGTG GTTTCCCAGG AGGTCCAGGT
    TTTTCGGGTA CACCAGGAAT ACCAGGTCCC CCAGGATTAC CAGGTGTGAA AGGAGATCCA
    GGTGAATCTT TCCCTTTAGC ACCGGGACAA AAAGGTGATC CAGGATTACC AGGTCTTCCA
    GGTTTACCAG GTCGGACTGG TCCTATTGGA GTTCAAGGTC CTGTGGGTCG ACCTGGTCCT
    GCTGGCTTAC CGGGGTCACC AGGAGTACCT GGACCTCCAG GACCCAAGGG TAACATGGGG
    CTGAATTTCC AGGGATCAAA GGGAGAAAAG GGTGAACCTG GTATTCAAGG ACTACCAGGA
    CCACCGGGAC AACTCGAGCA AGTTGAACCT CCACAAACTA CAATTCAGAA GGGAGAGAAG
    GGTATCCCCG GTGACCAAGG ATTTCCAGGA GAGCGTGGGC CAATGGGTCC TCCTGGTCCT
    CCTGGAGGTG CAAAGGGAGA GAAGGGAGAA CCAGGGGAAC CTGGAAAGAT TGGGAAGCCT
    GGAAAAGATG GAAAGATTGG TCTGCCCGGT GGAGAGGGTT TCAGGGGTGA CCCAGGCTTG
    CCGGGAGGTC CAGGCAGAGA TGGGGACAAG GGTCAAAAAG GTGAAAATGG GCTCCCTGGG
    CCACCAGGAG AGGTAATTTC AAGACCAGGG ACAGGCAGAG TTGGACCAAA AGGAAACACA
    GGACCCCCTG GAATAACAGG GCCAAAAGGA GACCGTGGAT TCCCTGGATT ACAAGGTCCC
    CCTGGTTTTC CTGGAACACC AGGTACTTCA AGTGTAGGCC CATCAGGAAA TCCAGGCCTT
    CCTGGTGAAA GAGGGCAAAA AGGCGATGAT GGTCCACCAG GAATCTCTAT TCCAGGGCTT
    CCAGGTTCTG AAGGTCTTGC AGGACCCCCT GGTCCCCCAG GTTTGCCTGG TCGACCTGGA
    ACATCTGCAG TTGGTCCTGC AATGGTATGC CAGCCAGGGC TACCTGGTAC TCAAGGACTT
    CCAGGAGATC CAGGATTTTT AGGAGAAAGA GGACAGAAAG GTGACAAAGG AGACACATGT
    TATAACTGCA TAGGAAATGG TATTTTAGGT CCGAAGGGGG ATCCTGGTTT GCCTGGTTTC
    CCTGGAAGTC CAGGTTCACC TGGAGCTCCA GGGCAAAAAG GAGCAATGGG TATCCCAGGA
    ACAAATGGGC TTCCAGGACC TCCGGGTTTT CCTGGCAATC CTGGTATCTC TGGCCGACCC
    GGTCCCAAAG GAGAACCTGG AGAAGTAATT GGCGAAATTA AAGGAGAAAA GGGGGACCCT
    GGATTCCCAG GTCCAGCTGG ATCTCCTGGT CTGGATGGTG TCCCTGGAAG AGACGGAACC
    CCAGGGATCC CTGGCCCTAA GGGAGAGCCG GGTGGCTTCG CGTTGAAAGG CGATCGTGGT
    TTTCCTGGTG ACATTGGGTT CCCTGGACAT CCTGGAGATC GAGGACTAAT GGGTCCTCCT
    GGTTTTGGTC CCAGTGGTCC ACCTGGAGAG AAGGGAATTC AAGGTGTTTC TGGTCGTCCA
    GGAGCCCCTG GAATACCTGG CCCTAAAGGA TCTCCTGGAC AGACTGTGAT GGAAAAAGGG
    GATTTTGGAC CCCCTGGTCT CCCAGGTATC CCAGGGGATG CCGGAACTCC AGGTGAACCA
    GGTCTACCTG GTCAGCCAGG AATTCCAGGT GTTCCAGGTT CAAAAGGAGA ACCAGGCATT
    CCAGGCATTG GACTTCCTGG GTCTGCAGGC GCAAAAGGAT TTCCTGGTAA TACTGGGCCA
    CCAGGTGCAC CAGGGTTGGC AGGAAGACCT GGTTCAGATG GGTCACCAGG ACAGCCTGGC
    TTGCCAGGAC CAAAGGGTGA CCCTGGGTTT GGTTTACCTG GGCTACCAGG CTCCCCAGGC
    CCTGCTGGAT TGAAAGGATC CCAAGGACCA AAAGGAGAAT CAGGCTTCCC AGGTTCTCCA
    GGCCTTCCTG GACGAGCAGG ATTTGATGGG CCTGTGGGAC CAAAAGGTGA CTCTGGACAA
    AATGGCTTAC CAGGAACCCC TGGACCACCT GGGGTACCTG GTGCTGGTGG TCAAGGCCCT
    CCAGGACAAC CAGGGCTTCC AGGGCCAGCA GGCCCTCCAG GTCCCACAGG GAACACTGGA
    ATACAGGGAG AGAAAGGAGA TCCTGGTGCT CCAGGGTTTG ACATGCCAGG CCCACCAGGA
    GATCGTGGTA GCCCTGGATT TCCAGGAGAA AGAGGGTTTA CAGGACCACC AGGGCCACCT
    GGAGCCCAAG GTCGCGATGG ATTACCAGGC TTCTCAGGTG CTAAAGGTGA AATGGGTGTA
    ATGGGACCAC CAGGATCTGT CGGACCAGCT GGTAATCCTG GAAACCCTGG TTTACCTGGG
    TCTAAAGGAA ATGATGGGAA TCCTGGACAA CCTGGCCTGT CTGGTTTACC AGGGGCTGAA
    GGTGAAAAGG GAGAGAGAGG ATTACCAGGA CCCCCTGGAA GAGTTGATTC TGCAAACCTA
    CAGTTTAAAG GAGTTAAAGG AGAACCTGGC TTGCCAGGTT CATCAGGACT AAGTGGGGCA
    AAAGGTGCTG CTGGACTTCC TGGTAACTCA GGATCCCCAG GGCAGATTGG AATTCCAGGA
    TCTCCAGGTC TTCCAGGGCT CAAGGGAGAT TCTGGCCTTC CTGGTCAGCC GGGTACTTCA
    GGTCCTCCTG GATTAAAAGG GAGCATGGGA GAGATGGGAC TTCCTGGGCC TCCTGGTTCT
    AAAGGTTCCC CTGGACAGCC AGGAAGGCCT GGTTCAGCAG GGTCTCCTGG GTTTTCTGGA
    CTGAAAGGTG AAAAAGGTGA ACCAGGAATT TCAGGAGCTG GTATCCCAGG CTTTCCTGGG
    CAGAAGGGTG AACCAGGGCA AAATGGCTTT CCAGGCAGCC CTGGTAGTAA AGGCAACCCA
    GGAAACCCAG GACTGCCCGG CCTACCAGGG ATTTCAGGAC CTAAAGGGGA TCCAGGACTT
    CCAGGATTCC CAGGCAGCCC TGGATCTCCA GGCCTTAAAG GAGTTGATGG GCCCACAGGA
    AACTCTGGGT TACCCGGATC TCCAGGCTTA CGGGGTGAAT CAGGTCGTCC CGGACCTCAA
    GGAACTCCTG GTGAGAAGGG TCAACCAGGT CAGGATGGAA TTCCTGGGCC TTCTGGTTTG
    AAGGGAGAAC CAGGTTTGCC AGGTTTTGGA TCCCCAGGGC TGCCTGGCCT TCCAGGAAGT
    CAAGGTCAAA AGGGTGATGC AGGACTTTCT GGAAGCCCTG GAAACCCCGG ACTTCCAGGG
    TTCAAAGGAG ATCAAGGCTT TCCAGGATCT CCTGGGCCAC AAGGACCAAC AGGACAACCT
    GGCCTACCAG GGCCACGTAC AGAAGGACCC AAGGGAGACC CTGGGCCTGT AGGTCCCCCT
    GGAAGACCAG GTCCTCCTGG CTCAGAAGGT CCACGTGGTC CCCCTGGTGA TGGAGGCTTT
    AAAGGAGAAA AAGGAAACCC TGGATTTCCA GGTCAGCCTG GACTTACAGG ATCTAAAGGA
    GAACATGGAG CATCTGGTTT CCCAGGTGAC CCAGGACGAC CAGGTCTTAA TGGGATGAAA
    GGTGATCAAG GTGTTCCTGG TGTTCCAGGA TTCCCAGGTA TGAAAGGACC AGGTGGCCCT
    CCTGGATTGG CTGGCCTTAA AGGTGAATCC GGACTCCCAG GTGCTATAGG TTTGCCAGGT
    CTTCCTGGGC CTAAAGGAGA ATACCGTGAG ATAAAAGGAG CTCCAGGTCT GCCAGGTCCT
    CCAGGTCGCC CAGGAGTAAA TGGACCTCCA GGACCCCCCG GATCACCAGG ACTTCCAGGT
    TCTTTGGGTC TCCCTGGTGA TACTGGCAGA GATGGACTCC CTGGATTTGA GGGACAACTT
    GGACGAAAGG GTGACCGAGG CCTACCAGGC CAACCAGGAT CTCGTGGAAT TAATGGCCCA
    CCTGGCCCAG ATGGTTTACA AGGCCCCCCT GGCCCCCCTG GTCTAGGATC TGTTGCTCAT
    GGATTCCTTA TAACCCGACA CAGTCAGTCA ACAGATGCCC CTGGCTGTCC TTTTGGAACC
    ACCAGGATCT ATGATGGCTA TTCACTACTT TATGTGCAAG GAAATGAGAG GGCACACGGA
    CAAGATTTGG GTACCGCTGG CAGCTGCCTC CGTCGCTTCA GCACAATGCC TTTCATGTTC
    TGCAACATTA ATAACGTGTG TAACTTTGCT TCAAGAAATG ACTACTCCTA CTGGTTGTCC
    ACGCCTGAGC CTATGCCTAT GAGCATGGAA CCTTTAAGAG GGAGGGATAT CCAACCATTC
    ATTAGCAGGT GTGCGGTCTG TGAAGCGCCA GCCATGGTGA TTGCAGTTCA CAGCCAGACA
    ATACAAATCC CACAGTGCCC ACAAGGCTGG TCCTCTCTCT GGATTGGATA CTCTTTCATG
    ATGCATACAA GTGCCGGTGC TGAAGGCTCA GGACAAGCTC TGGCATCGCC TGGATCGTGC
    TTGGAGGAAT TCCGATCTGC ACCTTTCATT GAATGTCATG GCCGGGGAAC CTGCAATTAC
    TATGCCAATT CATACAGTTT TTGGTTGGCC ACAGTGGAAA TGTCTGAAAT GTTTAGCAAA
    CCTCAGTCAG AAACACTAAA AGCGGGTGAT CTAAGGACAC GCGTCAGTCG CTGCCAAGTT
    TGTATGAAGA GGACGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004916925.1
    CDS289..5346
    Translation

    Target ORF information:

    RefSeq Version XM_004916868.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis collagen, type IV, alpha 5 (col4a5), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004916868.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAGACTGG GAAGTTGGCA GCGGATGCCC TGCCTGGCTG TACTGGTGTT GGTGCTGGGG 
    CACTGGGCAC AGAATGCAGA GTCTGCGGCC TGTCAAGGTT GTACATCGGT ATCGAAATGT
    GACTGCAGTG GTGTTAAAGG AGAAAAGGGT GAGAGAGGCC TTCCAGGGTT AGAAGGACAC
    CTTGGTCTAC CAGGATTTCC TGGACCTGAA GGACCGCCTG GACCTCGTGG TCCCAAGGGT
    GATTCTGGTT TATCAGGGTC TCCAGGACCC AAAGGAATAC GAGGACCTCC TGGACCACCC
    GGATTTCCAG GAAACCCTGG CCAGCCTGGC CTGCCGGGAC ATGATGGTGA ACCAGGGCCT
    GCAGGTATCC CAGGATGCAA TGGCACAAAG GGAGATCGTG GTTTCCCAGG AGGTCCAGGT
    TTTTCGGGTA CACCAGGAAT ACCAGGTCCC CCAGGATTAC CAGGTGTGAA AGGAGATCCA
    GGTGAATCTT TCCCTTTAGC ACCGGGACAA AAAGGTGATC CAGGATTACC AGGTCTTCCA
    GGTTTACCAG GTCGGACTGG TCCTATTGGA GTTCAAGGTC CTGTGGGTCG ACCTGGTCCT
    GCTGGCTTAC CGGGGTCACC AGGAGTACCT GGACCTCCAG GACCCAAGGG TAACATGGGG
    CTGAATTTCC AGGGATCAAA GGGAGAAAAG GGTGAACCTG GTATTCAAGG ACTACCAGGA
    CCACCGGGAC AACTCGAGCA AGTTGAACCT CCACAAACTA CAATTCAGAA GGGAGAGAAG
    GGTATCCCCG GTGACCAAGG ATTTCCAGGA GAGCGTGGGC CAATGGGTCC TCCTGGTCCT
    CCTGGAGGTG CAAAGGGAGA GAAGGGAGAA CCAGGGGAAC CTGGAAAGAT TGGGAAGCCT
    GGAAAAGATG GAAAGATTGG TCTGCCCGGT GGAGAGGGTT TCAGGGGTGA CCCAGGCTTG
    CCGGGAGGTC CAGGCAGAGA TGGGGACAAG GGTCAAAAAG GTGAAAATGG GCTCCCTGGG
    CCACCAGGAG AGGTAATTTC AAGACCAGGG ACAGGCAGAG TTGGACCAAA AGGAAACACA
    GGACCCCCTG GAATAACAGG GCCAAAAGGA GACCGTGGAT TCCCTGGATT ACAAGGTCCC
    CCTGGTTTTC CTGGAACACC AGGTACTTCA AGTGTAGGCC CATCAGGAAA TCCAGGCCTT
    CCTGGTGAAA GAGGGCAAAA AGGCGATGAT GGTCCACCAG GAATCTCTAT TCCAGGGCTT
    CCAGGTTCTG AAGGTCTTGC AGGACCCCCT GGTCCCCCAG GTTTGCCTGG TCGACCTGGA
    ACATCTGCAG TTGGTCCTGC AATGGTATGC CAGCCAGGGC TACCTGGTAC TCAAGGACTT
    CCAGGAGATC CAGGATTTTT AGGAGAAAGA GGACAGAAAG GTGACAAAGG AGACACATGT
    TATAACTGCA TAGGAAATGG TATTTTAGGT CCGAAGGGGG ATCCTGGTTT GCCTGGTTTC
    CCTGGAAGTC CAGGTTCACC TGGAGCTCCA GGGCAAAAAG GAGCAATGGG TATCCCAGGA
    ACAAATGGGC TTCCAGGACC TCCGGGTTTT CCTGGCAATC CTGGTATCTC TGGCCGACCC
    GGTCCCAAAG GAGAACCTGG AGAAGTAATT GGCGAAATTA AAGGAGAAAA GGGGGACCCT
    GGATTCCCAG GTCCAGCTGG ATCTCCTGGT CTGGATGGTG TCCCTGGAAG AGACGGAACC
    CCAGGGATCC CTGGCCCTAA GGGAGAGCCG GGTGGCTTCG CGTTGAAAGG CGATCGTGGT
    TTTCCTGGTG ACATTGGGTT CCCTGGACAT CCTGGAGATC GAGGACTAAT GGGTCCTCCT
    GGTTTTGGTC CCAGTGGTCC ACCTGGAGAG AAGGGAATTC AAGGTGTTTC TGGTCGTCCA
    GGAGCCCCTG GAATACCTGG CCCTAAAGGA TCTCCTGGAC AGACTGTGAT GGAAAAAGGG
    GATTTTGGAC CCCCTGGTCT CCCAGGTATC CCAGGGGATG CCGGAACTCC AGGTGAACCA
    GGTCTACCTG GTCAGCCAGG AATTCCAGGT GTTCCAGGTT CAAAAGGAGA ACCAGGCATT
    CCAGGCATTG GACTTCCTGG GTCTGCAGGC GCAAAAGGAT TTCCTGGTAA TACTGGGCCA
    CCAGGTGCAC CAGGGTTGGC AGGAAGACCT GGTTCAGATG GGTCACCAGG ACAGCCTGGC
    TTGCCAGGAC CAAAGGGTGA CCCTGGGTTT GGTTTACCTG GGCTACCAGG CTCCCCAGGC
    CCTGCTGGAT TGAAAGGATC CCAAGGACCA AAAGGAGAAT CAGGCTTCCC AGGTTCTCCA
    GGCCTTCCTG GACGAGCAGG ATTTGATGGG CCTGTGGGAC CAAAAGGTGA CTCTGGACAA
    AATGGCTTAC CAGGAACCCC TGGACCACCT GGGGTACCTG GTGCTGGTGG TCAAGGCCCT
    CCAGGACAAC CAGGGCTTCC AGGGCCAGCA GGCCCTCCAG GTCCCACAGG GAACACTGGA
    ATACAGGGAG AGAAAGGAGA TCCTGGTGCT CCAGGGTTTG ACATGCCAGG CCCACCAGGA
    GATCGTGGTA GCCCTGGATT TCCAGGAGAA AGAGGGTTTA CAGGACCACC AGGGCCACCT
    GGAGCCCAAG GTCGCGATGG ATTACCAGGC TTCTCAGGTG CTAAAGGTGA AATGGGTGTA
    ATGGGACCAC CAGGATCTGT CGGACCAGCT GGTAATCCTG GAAACCCTGG TTTACCTGGG
    TCTAAAGGAA ATGATGGGAA TCCTGGACAA CCTGGCCTGT CTGGTTTACC AGGGGCTGAA
    GGTGAAAAGG GAGAGAGAGG ATTACCAGGA CCCCCTGGAA GAGTTGATTC TGCAAACCTA
    CAGTTTAAAG GAGTTAAAGG AGAACCTGGC TTGCCAGGTT CATCAGGACT AAGTGGGGCA
    AAAGGTGCTG CTGGACTTCC TGGTAACTCA GGATCCCCAG GGCAGATTGG AATTCCAGGA
    TCTCCAGGTC TTCCAGGGCT CAAGGGAGAT TCTGGCCTTC CTGGTCAGCC GGGTACTTCA
    GGTCCTCCTG GATTAAAAGG GAGCATGGGA GAGATGGGAC TTCCTGGGCC TCCTGGTTCT
    AAAGGTTCCC CTGGACAGCC AGGAAGGCCT GGTTCAGCAG GGTCTCCTGG GTTTTCTGGA
    CTGAAAGGTG AAAAAGGTGA ACCAGGAATT TCAGGAGCTG GTATCCCAGG CTTTCCTGGG
    CAGAAGGGTG AACCAGGGCA AAATGGCTTT CCAGGCAGCC CTGGTAGTAA AGGCAACCCA
    GGAAACCCAG GACTGCCCGG CCTACCAGGG ATTTCAGGAC CTAAAGGGGA TCCAGGACTT
    CCAGGATTCC CAGGCAGCCC TGGATCTCCA GGCCTTAAAG GAGTTGATGG GCCCACAGGA
    AACTCTGGGT TACCCGGATC TCCAGGCTTA CGGGGTGAAT CAGGTCGTCC CGGACCTCAA
    GGAACTCCTG GTGAGAAGGG TCAACCAGGT CAGGATGGAA TTCCTGGGCC TTCTGGTTTG
    AAGGGAGAAC CAGGTTTGCC AGGTTTTGGA TCCCCAGGGC TGCCTGGCCT TCCAGGAAGT
    CAAGGTCAAA AGGGTGATGC AGGACTTTCT GGAAGCCCTG GAAACCCCGG ACTTCCAGGG
    TTCAAAGGAG ATCAAGGCTT TCCAGGATCT CCTGGGCCAC AAGGACCAAC AGGACAACCT
    GGCCTACCAG GGCCACGTAC AGAAGGACCC AAGGGAGACC CTGGGCCTGT AGGTCCCCCT
    GGAAGACCAG GTCCTCCTGG CTCAGAAGGT CCACGTGGTC CCCCTGGTGA TGGAGGCTTT
    AAAGGAGAAA AAGGAAACCC TGGATTTCCA GGTCAGCCTG GACTTACAGG ATCTAAAGGA
    GAACATGGAG CATCTGGTTT CCCAGGTGAC CCAGGACGAC CAGGTCTTAA TGGGATGAAA
    GGTGATCAAG GTGTTCCTGG TGTTCCAGGA TTCCCAGGTA TGAAAGGACC AGGTGGCCCT
    CCTGGATTGG CTGGCCTTAA AGGTGAATCC GGACTCCCAG GTGCTATAGG TTTGCCAGGT
    CTTCCTGGGC CTAAAGGAGA ATACCGTGAG ATAAAAGGAG CTCCAGGTCT GCCAGGTCCT
    CCAGGTCGCC CAGGAGTAAA TGGACCTCCA GGACCCCCCG GATCACCAGG ACTTCCAGGT
    TCTTTGGGTC TCCCTGGTGA TACTGGCAGA GATGGACTCC CTGGATTTGA GGGACAACTT
    GGACGAAAGG GTGACCGAGG CCTACCAGGC CAACCAGGAT CTCGTGGAAT TAATGGCCCA
    CCTGGCCCAG ATGGTTTACA AGGCCCCCCT GGCCCCCCTG GTCTAGGATC TGTTGCTCAT
    GGATTCCTTA TAACCCGACA CAGTCAGTCA ACAGATGCCC CTGGCTGTCC TTTTGGAACC
    ACCAGGATCT ATGATGGCTA TTCACTACTT TATGTGCAAG GAAATGAGAG GGCACACGGA
    CAAGATTTGG GTACCGCTGG CAGCTGCCTC CGTCGCTTCA GCACAATGCC TTTCATGTTC
    TGCAACATTA ATAACGTGTG TAACTTTGCT TCAAGAAATG ACTACTCCTA CTGGTTGTCC
    ACGCCTGAGC CTATGCCTAT GAGCATGGAA CCTTTAAGAG GGAGGGATAT CCAACCATTC
    ATTAGCAGGT GTGCGGTCTG TGAAGCGCCA GCCATGGTGA TTGCAGTTCA CAGCCAGACA
    ATACAAATCC CACAGTGCCC ACAAGGCTGG TCCTCTCTCT GGATTGGATA CTCTTTCATG
    ATGCATACAA GTGCCGGTGC TGAAGGCTCA GGACAAGCTC TGGCATCGCC TGGATCGTGC
    TTGGAGGAAT TCCGATCTGC ACCTTTCATT GAATGTCATG GCCGGGGAAC CTGCAATTAC
    TATGCCAATT CATACAGTTT TTGGTTGGCC ACAGTGGAAA TGTCTGAAAT GTTTAGCAAA
    CCTCAGTCAG AAACACTAAA AGCGGGTGAT CTAAGGACAC GCGTCAGTCG CTGCCAAGTT
    TGTATGAAGA GGACGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa14364
    Clone ID Related Accession (Same CDS sequence) XM_004916869.3
    Accession Version XM_004916869.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5049bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product collagen alpha-5(IV) chain isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004916869.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAGACTGG GAAGTTGGCA GCGGATGCCC TGCCTGGCTG TACTGGTGTT GGTGCTGGGG 
    CACTGGGCAC AGAATGCAGA GTCTGCGGCC TGTCAAGGTT GTACATCGGT ATCGAAATGT
    GACTGCAGTG GTGTTAAAGG AGAAAAGGGT GAGAGAGGCC TTCCAGGGTT AGAAGGACAC
    CTTGGTCTAC CAGGATTTCC TGGACCTGAA GGACCGCCTG GACCTCGTGG TCCCAAGGGT
    GATTCTGGTT TATCAGGGTC TCCAGGACCC AAAGGAATAC GAGGACCTCC TGGACCACCC
    GGATTTCCAG GAAACCCTGG CCAGCCTGGC CTGCCGGGAC ATGATGGTGA ACCAGGGCCT
    GCAGGTATCC CAGGATGCAA TGGCACAAAG GGAGATCGTG GTTTCCCAGG AGGTCCAGGT
    TTTTCGGGTA CACCAGGAAT ACCAGGTCCC CCAGGATTAC CAGGTGTGAA AGGAGATCCA
    GGTGAATCTT TCCCTTTAGC ACCGGGACAA AAAGGTGATC CAGGATTACC AGGTCTTCCA
    GGTTTACCAG GTCGGACTGG TCCTATTGGA GTTCAAGGTC CTGTGGGTCG ACCTGGTCCT
    GCTGGCTTAC CGGGGTCACC AGGAGTACCT GGACCTCCAG GACCCAAGGG TAACATGGGG
    CTGAATTTCC AGGGATCAAA GGGAGAAAAG GGTGAACCTG GTATTCAAGG ACTACCAGGA
    CCACCGGGAC AACTCGAGCA AGTTGAACCT CCACAAACTA CAATTCAGAA GGGAGAGAAG
    GGTATCCCCG GTGACCAAGG ATTTCCAGGA GAGCGTGGGC CAATGGGTCC TCCTGGTCCT
    CCTGGAGGTG CAAAGGGAGA GAAGGGAGAA CCAGGGGAAC CTGGAAAGAT TGGGAAGCCT
    GGAAAAGATG GAAAGATTGG TCTGCCCGGT GGAGAGGGTT TCAGGGGTGA CCCAGGCTTG
    CCGGGAGGTC CAGGCAGAGA TGGGGACAAG GGTCAAAAAG GTGAAAATGG GCTCCCTGGG
    CCACCAGGAG AGGTAATTTC AAGACCAGGG ACAGGCAGAG TTGGACCAAA AGGAAACACA
    GGACCCCCTG GAATAACAGG GCCAAAAGGA GACCGTGGAT TCCCTGGATT ACAAGGTCCC
    CCTGGTTTTC CTGGAACACC AGGTACTTCA AGTGTAGGCC CATCAGGAAA TCCAGGCCTT
    CCTGGTGAAA GAGGGCAAAA AGGCGATGAT GGTCCACCAG GAATCTCTAT TCCAGGGCTT
    CCAGGTTCTG AAGGTCTTGC AGGACCCCCT GGTCCCCCAG GTTTGCCTGG TCGACCTGGA
    ACATCTGCAG TTGGTCCTGC AATGGTATGC CAGCCAGGGC TACCTGGTAC TCAAGGACTT
    CCAGGAGATC CAGGATTTTT AGGAGAAAGA GGACAGAAAG GTGACAAAGG AGACACATGT
    TATAACTGCA TAGGAAATGG TATTTTAGGT CCGAAGGGGG ATCCTGGTTT GCCTGGTTTC
    CCTGGAAGTC CAGGTTCACC TGGAGCTCCA GGGCAAAAAG GAGCAATGGG TATCCCAGGA
    ACAAATGGGC TTCCAGGACC TCCGGGTTTT CCTGGCAATC CTGGTATCTC TGGCCGACCC
    GGTCCCAAAG GAGAACCTGG AGAAGTAATT GGCGAAATTA AAGGAGAAAA GGGGGACCCT
    GGATTCCCAG GTCCAGCTGG ATCTCCTGGT CTGGATGGTG TCCCTGGAAG AGACGGAACC
    CCAGGGATCC CTGGCCCTAA GGGAGAGCCG GGTGGCTTCG CGTTGAAAGG CGATCGTGGT
    TTTCCTGGTG ACATTGGGTT CCCTGGACAT CCTGGAGATC GAGGACTAAT GGGTCCTCCT
    GGTTTTGGTC CCAGTGGTCC ACCTGGAGAG AAGGGAATTC AAGGTGTTTC TGGTCGTCCA
    GGAGCCCCTG GAATACCTGG CCCTAAAGGA TCTCCTGGAC AGACTGTGAT GGAAAAAGGG
    GATTTTGGAC CCCCTGGTCT CCCAGGTATC CCAGGGGATG CCGGAACTCC AGGTGAACCA
    GGTCTACCTG GTCAGCCAGG AATTCCAGGT GTTCCAGGTT CAAAAGGAGA ACCAGGCATT
    CCAGGCATTG GACTTCCTGG GTCTGCAGGC GCAAAAGGAT TTCCTGGTAA TACTGGGCCA
    CCAGGTGCAC CAGGGTTGGC AGGAAGACCT GGTTCAGATG GGTCACCAGG ACAGCCTGGC
    TTGCCAGGAC CAAAGGGTGA CCCTGGGTTT GGTTTACCTG GGCTACCAGG CTCCCCAGGC
    CCTGCTGGAT TGAAAGGATC CCAAGGACCA AAAGGAGAAT CAGGCTTCCC AGGTTCTCCA
    GGCCTTCCTG GACGAGCAGG ATTTGATGGG CCTGTGGGAC CAAAAGGTGA CTCTGGACAA
    AATGGCTTAC CAGGAACCCC TGGACCACCT GGGGTACCTG GTGCTGGTGG TCAAGGCCCT
    CCAGGACAAC CAGGGCTTCC AGGGCCAGCA GGCCCTCCAG GGAACACTGG AATACAGGGA
    GAGAAAGGAG ATCCTGGTGC TCCAGGGTTT GACATGCCAG GCCCACCAGG AGATCGTGGT
    AGCCCTGGAT TTCCAGGAGA AAGAGGGTTT ACAGGACCAC CAGGGCCACC TGGAGCCCAA
    GGTCGCGATG GATTACCAGG CTTCTCAGGT GCTAAAGGTG AAATGGGTGT AATGGGACCA
    CCAGGATCTG TCGGACCAGC TGGTAATCCT GGAAACCCTG GTTTACCTGG GTCTAAAGGA
    AATGATGGGA ATCCTGGACA ACCTGGCCTG TCTGGTTTAC CAGGGGCTGA AGGTGAAAAG
    GGAGAGAGAG GATTACCAGG ACCCCCTGGA AGAGTTGATT CTGCAAACCT ACAGTTTAAA
    GGAGTTAAAG GAGAACCTGG CTTGCCAGGT TCATCAGGAC TAAGTGGGGC AAAAGGTGCT
    GCTGGACTTC CTGGTAACTC AGGATCCCCA GGGCAGATTG GAATTCCAGG ATCTCCAGGT
    CTTCCAGGGC TCAAGGGAGA TTCTGGCCTT CCTGGTCAGC CGGGTACTTC AGGTCCTCCT
    GGATTAAAAG GGAGCATGGG AGAGATGGGA CTTCCTGGGC CTCCTGGTTC TAAAGGTTCC
    CCTGGACAGC CAGGAAGGCC TGGTTCAGCA GGGTCTCCTG GGTTTTCTGG ACTGAAAGGT
    GAAAAAGGTG AACCAGGAAT TTCAGGAGCT GGTATCCCAG GCTTTCCTGG GCAGAAGGGT
    GAACCAGGGC AAAATGGCTT TCCAGGCAGC CCTGGTAGTA AAGGCAACCC AGGAAACCCA
    GGACTGCCCG GCCTACCAGG GATTTCAGGA CCTAAAGGGG ATCCAGGACT TCCAGGATTC
    CCAGGCAGCC CTGGATCTCC AGGCCTTAAA GGAGTTGATG GGCCCACAGG AAACTCTGGG
    TTACCCGGAT CTCCAGGCTT ACGGGGTGAA TCAGGTCGTC CCGGACCTCA AGGAACTCCT
    GGTGAGAAGG GTCAACCAGG TCAGGATGGA ATTCCTGGGC CTTCTGGTTT GAAGGGAGAA
    CCAGGTTTGC CAGGTTTTGG ATCCCCAGGG CTGCCTGGCC TTCCAGGAAG TCAAGGTCAA
    AAGGGTGATG CAGGACTTTC TGGAAGCCCT GGAAACCCCG GACTTCCAGG GTTCAAAGGA
    GATCAAGGCT TTCCAGGATC TCCTGGGCCA CAAGGACCAA CAGGACAACC TGGCCTACCA
    GGGCCACGTA CAGAAGGACC CAAGGGAGAC CCTGGGCCTG TAGGTCCCCC TGGAAGACCA
    GGTCCTCCTG GCTCAGAAGG TCCACGTGGT CCCCCTGGTG ATGGAGGCTT TAAAGGAGAA
    AAAGGAAACC CTGGATTTCC AGGTCAGCCT GGACTTACAG GATCTAAAGG AGAACATGGA
    GCATCTGGTT TCCCAGGTGA CCCAGGACGA CCAGGTCTTA ATGGGATGAA AGGTGATCAA
    GGTGTTCCTG GTGTTCCAGG ATTCCCAGGT ATGAAAGGAC CAGGTGGCCC TCCTGGATTG
    GCTGGCCTTA AAGGTGAATC CGGACTCCCA GGTGCTATAG GTTTGCCAGG TCTTCCTGGG
    CCTAAAGGAG AATACCGTGA GATAAAAGGA GCTCCAGGTC TGCCAGGTCC TCCAGGTCGC
    CCAGGAGTAA ATGGACCTCC AGGACCCCCC GGATCACCAG GACTTCCAGG TTCTTTGGGT
    CTCCCTGGTG ATACTGGCAG AGATGGACTC CCTGGATTTG AGGGACAACT TGGACGAAAG
    GGTGACCGAG GCCTACCAGG CCAACCAGGA TCTCGTGGAA TTAATGGCCC ACCTGGCCCA
    GATGGTTTAC AAGGCCCCCC TGGCCCCCCT GGTCTAGGAT CTGTTGCTCA TGGATTCCTT
    ATAACCCGAC ACAGTCAGTC AACAGATGCC CCTGGCTGTC CTTTTGGAAC CACCAGGATC
    TATGATGGCT ATTCACTACT TTATGTGCAA GGAAATGAGA GGGCACACGG ACAAGATTTG
    GGTACCGCTG GCAGCTGCCT CCGTCGCTTC AGCACAATGC CTTTCATGTT CTGCAACATT
    AATAACGTGT GTAACTTTGC TTCAAGAAAT GACTACTCCT ACTGGTTGTC CACGCCTGAG
    CCTATGCCTA TGAGCATGGA ACCTTTAAGA GGGAGGGATA TCCAACCATT CATTAGCAGG
    TGTGCGGTCT GTGAAGCGCC AGCCATGGTG ATTGCAGTTC ACAGCCAGAC AATACAAATC
    CCACAGTGCC CACAAGGCTG GTCCTCTCTC TGGATTGGAT ACTCTTTCAT GATGCATACA
    AGTGCCGGTG CTGAAGGCTC AGGACAAGCT CTGGCATCGC CTGGATCGTG CTTGGAGGAA
    TTCCGATCTG CACCTTTCAT TGAATGTCAT GGCCGGGGAA CCTGCAATTA CTATGCCAAT
    TCATACAGTT TTTGGTTGGC CACAGTGGAA ATGTCTGAAA TGTTTAGCAA ACCTCAGTCA
    GAAACACTAA AAGCGGGTGA TCTAAGGACA CGCGTCAGTC GCTGCCAAGT TTGTATGAAG
    AGGACGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004916926.1
    CDS288..5336
    Translation

    Target ORF information:

    RefSeq Version XM_004916869.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis collagen, type IV, alpha 5 (col4a5), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004916869.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAGACTGG GAAGTTGGCA GCGGATGCCC TGCCTGGCTG TACTGGTGTT GGTGCTGGGG 
    CACTGGGCAC AGAATGCAGA GTCTGCGGCC TGTCAAGGTT GTACATCGGT ATCGAAATGT
    GACTGCAGTG GTGTTAAAGG AGAAAAGGGT GAGAGAGGCC TTCCAGGGTT AGAAGGACAC
    CTTGGTCTAC CAGGATTTCC TGGACCTGAA GGACCGCCTG GACCTCGTGG TCCCAAGGGT
    GATTCTGGTT TATCAGGGTC TCCAGGACCC AAAGGAATAC GAGGACCTCC TGGACCACCC
    GGATTTCCAG GAAACCCTGG CCAGCCTGGC CTGCCGGGAC ATGATGGTGA ACCAGGGCCT
    GCAGGTATCC CAGGATGCAA TGGCACAAAG GGAGATCGTG GTTTCCCAGG AGGTCCAGGT
    TTTTCGGGTA CACCAGGAAT ACCAGGTCCC CCAGGATTAC CAGGTGTGAA AGGAGATCCA
    GGTGAATCTT TCCCTTTAGC ACCGGGACAA AAAGGTGATC CAGGATTACC AGGTCTTCCA
    GGTTTACCAG GTCGGACTGG TCCTATTGGA GTTCAAGGTC CTGTGGGTCG ACCTGGTCCT
    GCTGGCTTAC CGGGGTCACC AGGAGTACCT GGACCTCCAG GACCCAAGGG TAACATGGGG
    CTGAATTTCC AGGGATCAAA GGGAGAAAAG GGTGAACCTG GTATTCAAGG ACTACCAGGA
    CCACCGGGAC AACTCGAGCA AGTTGAACCT CCACAAACTA CAATTCAGAA GGGAGAGAAG
    GGTATCCCCG GTGACCAAGG ATTTCCAGGA GAGCGTGGGC CAATGGGTCC TCCTGGTCCT
    CCTGGAGGTG CAAAGGGAGA GAAGGGAGAA CCAGGGGAAC CTGGAAAGAT TGGGAAGCCT
    GGAAAAGATG GAAAGATTGG TCTGCCCGGT GGAGAGGGTT TCAGGGGTGA CCCAGGCTTG
    CCGGGAGGTC CAGGCAGAGA TGGGGACAAG GGTCAAAAAG GTGAAAATGG GCTCCCTGGG
    CCACCAGGAG AGGTAATTTC AAGACCAGGG ACAGGCAGAG TTGGACCAAA AGGAAACACA
    GGACCCCCTG GAATAACAGG GCCAAAAGGA GACCGTGGAT TCCCTGGATT ACAAGGTCCC
    CCTGGTTTTC CTGGAACACC AGGTACTTCA AGTGTAGGCC CATCAGGAAA TCCAGGCCTT
    CCTGGTGAAA GAGGGCAAAA AGGCGATGAT GGTCCACCAG GAATCTCTAT TCCAGGGCTT
    CCAGGTTCTG AAGGTCTTGC AGGACCCCCT GGTCCCCCAG GTTTGCCTGG TCGACCTGGA
    ACATCTGCAG TTGGTCCTGC AATGGTATGC CAGCCAGGGC TACCTGGTAC TCAAGGACTT
    CCAGGAGATC CAGGATTTTT AGGAGAAAGA GGACAGAAAG GTGACAAAGG AGACACATGT
    TATAACTGCA TAGGAAATGG TATTTTAGGT CCGAAGGGGG ATCCTGGTTT GCCTGGTTTC
    CCTGGAAGTC CAGGTTCACC TGGAGCTCCA GGGCAAAAAG GAGCAATGGG TATCCCAGGA
    ACAAATGGGC TTCCAGGACC TCCGGGTTTT CCTGGCAATC CTGGTATCTC TGGCCGACCC
    GGTCCCAAAG GAGAACCTGG AGAAGTAATT GGCGAAATTA AAGGAGAAAA GGGGGACCCT
    GGATTCCCAG GTCCAGCTGG ATCTCCTGGT CTGGATGGTG TCCCTGGAAG AGACGGAACC
    CCAGGGATCC CTGGCCCTAA GGGAGAGCCG GGTGGCTTCG CGTTGAAAGG CGATCGTGGT
    TTTCCTGGTG ACATTGGGTT CCCTGGACAT CCTGGAGATC GAGGACTAAT GGGTCCTCCT
    GGTTTTGGTC CCAGTGGTCC ACCTGGAGAG AAGGGAATTC AAGGTGTTTC TGGTCGTCCA
    GGAGCCCCTG GAATACCTGG CCCTAAAGGA TCTCCTGGAC AGACTGTGAT GGAAAAAGGG
    GATTTTGGAC CCCCTGGTCT CCCAGGTATC CCAGGGGATG CCGGAACTCC AGGTGAACCA
    GGTCTACCTG GTCAGCCAGG AATTCCAGGT GTTCCAGGTT CAAAAGGAGA ACCAGGCATT
    CCAGGCATTG GACTTCCTGG GTCTGCAGGC GCAAAAGGAT TTCCTGGTAA TACTGGGCCA
    CCAGGTGCAC CAGGGTTGGC AGGAAGACCT GGTTCAGATG GGTCACCAGG ACAGCCTGGC
    TTGCCAGGAC CAAAGGGTGA CCCTGGGTTT GGTTTACCTG GGCTACCAGG CTCCCCAGGC
    CCTGCTGGAT TGAAAGGATC CCAAGGACCA AAAGGAGAAT CAGGCTTCCC AGGTTCTCCA
    GGCCTTCCTG GACGAGCAGG ATTTGATGGG CCTGTGGGAC CAAAAGGTGA CTCTGGACAA
    AATGGCTTAC CAGGAACCCC TGGACCACCT GGGGTACCTG GTGCTGGTGG TCAAGGCCCT
    CCAGGACAAC CAGGGCTTCC AGGGCCAGCA GGCCCTCCAG GGAACACTGG AATACAGGGA
    GAGAAAGGAG ATCCTGGTGC TCCAGGGTTT GACATGCCAG GCCCACCAGG AGATCGTGGT
    AGCCCTGGAT TTCCAGGAGA AAGAGGGTTT ACAGGACCAC CAGGGCCACC TGGAGCCCAA
    GGTCGCGATG GATTACCAGG CTTCTCAGGT GCTAAAGGTG AAATGGGTGT AATGGGACCA
    CCAGGATCTG TCGGACCAGC TGGTAATCCT GGAAACCCTG GTTTACCTGG GTCTAAAGGA
    AATGATGGGA ATCCTGGACA ACCTGGCCTG TCTGGTTTAC CAGGGGCTGA AGGTGAAAAG
    GGAGAGAGAG GATTACCAGG ACCCCCTGGA AGAGTTGATT CTGCAAACCT ACAGTTTAAA
    GGAGTTAAAG GAGAACCTGG CTTGCCAGGT TCATCAGGAC TAAGTGGGGC AAAAGGTGCT
    GCTGGACTTC CTGGTAACTC AGGATCCCCA GGGCAGATTG GAATTCCAGG ATCTCCAGGT
    CTTCCAGGGC TCAAGGGAGA TTCTGGCCTT CCTGGTCAGC CGGGTACTTC AGGTCCTCCT
    GGATTAAAAG GGAGCATGGG AGAGATGGGA CTTCCTGGGC CTCCTGGTTC TAAAGGTTCC
    CCTGGACAGC CAGGAAGGCC TGGTTCAGCA GGGTCTCCTG GGTTTTCTGG ACTGAAAGGT
    GAAAAAGGTG AACCAGGAAT TTCAGGAGCT GGTATCCCAG GCTTTCCTGG GCAGAAGGGT
    GAACCAGGGC AAAATGGCTT TCCAGGCAGC CCTGGTAGTA AAGGCAACCC AGGAAACCCA
    GGACTGCCCG GCCTACCAGG GATTTCAGGA CCTAAAGGGG ATCCAGGACT TCCAGGATTC
    CCAGGCAGCC CTGGATCTCC AGGCCTTAAA GGAGTTGATG GGCCCACAGG AAACTCTGGG
    TTACCCGGAT CTCCAGGCTT ACGGGGTGAA TCAGGTCGTC CCGGACCTCA AGGAACTCCT
    GGTGAGAAGG GTCAACCAGG TCAGGATGGA ATTCCTGGGC CTTCTGGTTT GAAGGGAGAA
    CCAGGTTTGC CAGGTTTTGG ATCCCCAGGG CTGCCTGGCC TTCCAGGAAG TCAAGGTCAA
    AAGGGTGATG CAGGACTTTC TGGAAGCCCT GGAAACCCCG GACTTCCAGG GTTCAAAGGA
    GATCAAGGCT TTCCAGGATC TCCTGGGCCA CAAGGACCAA CAGGACAACC TGGCCTACCA
    GGGCCACGTA CAGAAGGACC CAAGGGAGAC CCTGGGCCTG TAGGTCCCCC TGGAAGACCA
    GGTCCTCCTG GCTCAGAAGG TCCACGTGGT CCCCCTGGTG ATGGAGGCTT TAAAGGAGAA
    AAAGGAAACC CTGGATTTCC AGGTCAGCCT GGACTTACAG GATCTAAAGG AGAACATGGA
    GCATCTGGTT TCCCAGGTGA CCCAGGACGA CCAGGTCTTA ATGGGATGAA AGGTGATCAA
    GGTGTTCCTG GTGTTCCAGG ATTCCCAGGT ATGAAAGGAC CAGGTGGCCC TCCTGGATTG
    GCTGGCCTTA AAGGTGAATC CGGACTCCCA GGTGCTATAG GTTTGCCAGG TCTTCCTGGG
    CCTAAAGGAG AATACCGTGA GATAAAAGGA GCTCCAGGTC TGCCAGGTCC TCCAGGTCGC
    CCAGGAGTAA ATGGACCTCC AGGACCCCCC GGATCACCAG GACTTCCAGG TTCTTTGGGT
    CTCCCTGGTG ATACTGGCAG AGATGGACTC CCTGGATTTG AGGGACAACT TGGACGAAAG
    GGTGACCGAG GCCTACCAGG CCAACCAGGA TCTCGTGGAA TTAATGGCCC ACCTGGCCCA
    GATGGTTTAC AAGGCCCCCC TGGCCCCCCT GGTCTAGGAT CTGTTGCTCA TGGATTCCTT
    ATAACCCGAC ACAGTCAGTC AACAGATGCC CCTGGCTGTC CTTTTGGAAC CACCAGGATC
    TATGATGGCT ATTCACTACT TTATGTGCAA GGAAATGAGA GGGCACACGG ACAAGATTTG
    GGTACCGCTG GCAGCTGCCT CCGTCGCTTC AGCACAATGC CTTTCATGTT CTGCAACATT
    AATAACGTGT GTAACTTTGC TTCAAGAAAT GACTACTCCT ACTGGTTGTC CACGCCTGAG
    CCTATGCCTA TGAGCATGGA ACCTTTAAGA GGGAGGGATA TCCAACCATT CATTAGCAGG
    TGTGCGGTCT GTGAAGCGCC AGCCATGGTG ATTGCAGTTC ACAGCCAGAC AATACAAATC
    CCACAGTGCC CACAAGGCTG GTCCTCTCTC TGGATTGGAT ACTCTTTCAT GATGCATACA
    AGTGCCGGTG CTGAAGGCTC AGGACAAGCT CTGGCATCGC CTGGATCGTG CTTGGAGGAA
    TTCCGATCTG CACCTTTCAT TGAATGTCAT GGCCGGGGAA CCTGCAATTA CTATGCCAAT
    TCATACAGTT TTTGGTTGGC CACAGTGGAA ATGTCTGAAA TGTTTAGCAA ACCTCAGTCA
    GAAACACTAA AAGCGGGTGA TCTAAGGACA CGCGTCAGTC GCTGCCAAGT TTGTATGAAG
    AGGACGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.