Col4a5 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Col4a5
Entrez Gene ID	363457
Full Name	collagen type IV alpha 5 chain
Synonyms	RGD1565499
General protein information	Preferred Names collagen type IV alpha 5 chain Names collagen alpha-5(IV) chain collagen, type IV, alpha 5 procollagen, type IV, alpha 5
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: X Map Location: Xq33

mRNA and Protein(s)

mRNA	Protein	Name
XM_343778.6	XP_343779.2	collagen alpha-5(IV) chain isoform X1
XM_006257313.1	XP_006257375.1	collagen alpha-5(IV) chain isoform X4
XM_001055156.4	XP_001055156.1	collagen alpha-5(IV) chain isoform X1
XM_006227490.1	XP_006227552.1	collagen alpha-5(IV) chain isoform X2
XM_008758534.1	XP_008756756.1	collagen alpha-5(IV) chain isoform X3
XM_008773475.1	XP_008771697.1	collagen alpha-5(IV) chain isoform X3
XM_006257312.1	XP_006257374.1	collagen alpha-5(IV) chain isoform X2
XM_006227491.1	XP_006227553.1	collagen alpha-5(IV) chain isoform X4

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Col4a5	XP_343779.2
Danio rerio (zebrafish)	col4a5	NP_001116702.1
Xenopus tropicalis (tropical clawed frog)	col4a5	XP_002931863.2

Gene Ontology

The following Col4a5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Col4a5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa21617	XM_343778.6 Latest version!	Rattus norvegicus collagen type IV alpha 5 chain (Col4a5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
ORa21617	XM_001055156.4 Latest version!	Rattus norvegicus collagen type IV alpha 5 chain (Col4a5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
ORa21618	XM_006257312.1 Latest version!	Rattus norvegicus collagen type IV alpha 5 chain (Col4a5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
ORa21618	XM_006227490.1 Latest version!	Rattus norvegicus collagen type IV alpha 5 chain (Col4a5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
ORa21619	XM_006227491.1 Latest version!	Rattus norvegicus collagen type IV alpha 5 chain (Col4a5), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
ORa21619	XM_006257313.1 Latest version!	Rattus norvegicus collagen type IV alpha 5 chain (Col4a5), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
ORa48575	XM_008758534.1 Latest version!	Rattus norvegicus collagen type IV alpha 5 chain (Col4a5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
ORa48575	XM_008773475.1 Latest version!	Rattus norvegicus collagen type IV alpha 5 chain (Col4a5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Col4a5 ( XM_343778.6 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_343779.2 Rattus norvegicus collagen type IV alpha 5 chain (Col4a5), transcript variant X1, mRNA.

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CloneID	ORa21617
Clone ID Related Accession (Same CDS sequence)	XM_343778.6 , XM_001055156.4
Accession Version	XM_343778.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5076bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	collagen alpha-5(IV) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 9, 2013 this sequence version replaced XM_343778.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGTGC CTGGAGTGTG CCTGGCCGCC GGCTGGTTCT TACTGGCCCT GTGTCTGTGG 
GGGCAGCCGG GAGAGGCTGC GGCCTGCCAT GGGTGTTCTT CTGGATCAAA ATGTGACTGC 
AGTGGTATAA AAGGAGAAAA GGGAGAACGG GGCTTTCCAG GCTTAGAAGG CCATCCAGGT 
TTACCAGGAT TTCCAGGTCC AGAAGGGCCT CCAGGACCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGA ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGCCTTCC TGGGATGCCA GGCCACGATG GGGCTCCAGG ACCTCAAGGT 
ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGCGGATTTC CAGGCAGTCC AGGCTTTCCC 
GGTTTACAAG GACCTCCAGG ACCACCTGGG ATCCCAGGTA TGAAGGGGGA GCCAGGCAGT 
ATAATCATGT CATCTCTGCC AGGGCCAAAG GGTAATCAAG GATACCCAGG TCCTCCTGGA 
ATACAAGGTC CACCTGGTCC CAAGGGTGTA TCAGGGCCAA GTGGTCCTCC AGGACCACCA 
GGTTTAATGG GCCCTCCTGG CCCACCAGGC CCACCGGGAC CAAAGGGGAA TATGGGCTTA 
AATTTCCAGG GACCCAAAGG CGAAAAAGGT GAACAAGGTC TTCAGGGTCC ACCTGGTCCA 
CCTGGGCAGA TCAGTGAACA GAAAAGACCA ATTGACCTAG ATTTTCAGAA AGGCGATCAG 
GGAGTTCCCG GTGATAGAGG GCCTCCTGGA CCTCCGGGAA TACCTGGTCC TCCCGGTCCT 
CCTGGTGGTG TGAAAGGTGA AAAGGGTGAG CCCGGAGAGC CAGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAGACTGG TCAACCAGGA ATCCCAGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGTGAAC CAGGAAGGCA TGGTGAAAAG GGCCAAAAGG GTGATACTGG CCCACCTGGA 
CCACCTGGAT TTGTAATTCC TACACCTGGA ACTGGTGTGA CTATTGGAGC AAAAGGAAAT 
ATTGGGCAAC CTGGTTTGCC TGGAGAAAAA GGAGAGCGAG GATTTCCTGG CATACAAGGT 
CCACCTGGCT TTCCTGGACC TCCAGGGACA GCTGTCATGG GTCCCCCTGG TCCTCCTGGA 
TATCCTGGTG AAAGGGGCCA GAAAGGTGAT GAAGGTCCCC CTGGAATTTG TATTCCTGGA 
TCTCCTGGAC TTGATGGACA GCCTGGAGCT CCTGGCTTTC CAGGACCTCC TGGTCCTCCT 
GGCCCCCAGT TACCCCCGAG TGATGAAATC TGTAAAGCAG GCCCTCCAGG GCCCCCAGGA 
TCTCCAGGTG ACCAAGGACT CCAAGGAGAG CAAGGAGTAA AAGGTGACAA AGGTGATACT 
TGCTTCAACT GTATTGGAAC TGGCATTTCA GGGCCTCCAG GCCAACCAGG TTTACCAGGT 
CTCCCAGGTC CTCCAGGATC TCTTGGAATC CCTGGAGAGA AGGGCGAGAA AGGACAAGCT 
GGGACAACTG GTCCAAAAGG ATTGCCAGGC ATTCCTGGAC CTCCAGGTGC TCCAGGCTTT 
CCAGGGTCTA AGGGTGACCC TGGTGATGTT CTCACTCTTC CGGGAATGAA AGGTGAAAAA 
GGAGAATTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTC TACCTGGTAG TCCTGGAAAG 
GATGGTTTGC CAGGACTTCC TGGCCCCAAA GGAGAGCCCG GTGGAATCAC TTTTAAGGGT 
GAAAGGGGTC CTCCTGGGAA CCCAGGTTTA CCAGGTCTCC CAGGAAATAT GGGACCTACA 
GGCCCTGTTG GTTTTGGGCC TCCAGGCCCC GTAGGTGAAA AAGGCATACA AGGTGTGGCG 
GGAAATCCAG GCCAGCCAGG ATTACCAGGT CCTAAAGGTG ATCCAGGCCA GACCATCAGC 
CAGCCAGGAA AGCCTGGCTT GCCAGGGAAT CCAGGCAGAG ATGGTGAAGT GGGTCTTCCA 
GGTGATCCTG GACTTCCAGG CCAACCAGGC CTACCAGGAA TACCTGGTAG CAAAGGAGAA 
CCAGGTATCC CTGGGATTGG GCCTCCAGGA CCACCTGGTC CCAAAGGTTT TCCAGGAATT 
CCTGGACCTC CAGGACCTCC TGGGGCCCCT GGAAGAATGG GCCCAGAAGG GCCTCCTGGG 
TTACCGGGCT TTCCAGGACC CAAGGGAGAG CCAGGATTTG GGTTACCTGG GCCACCTGGG 
CCACCAGGAT TCCCAGGTTT CAAAGGAACA CCTGGTCCAA AAGGTGATCG TGGTTTACCA 
GGACCTCCAG GTCCTCCAGG ACACACTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAT 
GCTGGACCAA ATGGACAACC TGGCCCAATA GGGCCACCAG GGCTGCCAGG AATTGGTCTT 
CAGGGACCAC CAGGACCACC AGGGACTCCA GGGCCAATAG GCCAACCTGG CTTACATGGA 
ATACCAGGAG AGAAGGGAGA TCCAGGACCT CCTGGGCTTG ATGTTCCAGG ACTCCCTGGA 
GAGAGAGGTA GTCCAGGGCT CCCTGGAGCC CCTGGTCTTA TAGGACCTCC AGGATCACCT 
GGTATCCCTG GAAGAGCAGG TGTCCCTGGA TTTCCAGGTG TCAAAGGCGA AATGGGTATG 
ATGGGACCTC CAGGCCCACC GGGACCTTTG GGTACTCCTG GCAGGAGTGG TGCTCCTGGT 
CTTAAAGGTG ATGACGGCAT GCAAGGTCAG CCAGGTCTCC CTGGCCCTGT AGGAGAAAAG 
GGTGGTAAGG GAGAACCTGG CCTTCCAGGT CCTCCTGGAC CAATGCGTCC AGATTTCCTG 
GGATCAAAAG GAGAAAAGGG TGATCCTGGC TTACCAGGTA TTCCTGGACT TTCCGGACCA 
AAAGGCTACC AGGGATTACC TGGAGACCCA GGGCAACCTG GGCTGAGTGG ACAGCCGGGA 
TCACCAGGAC TACCAGGTCC CAAAGGTACT CCTGGTCTCC CTGGTCCACC AGGACTTACC 
GGACCTCCTG GACCTAAAGG AAACATTGGC GACATGGGTT TTCCTGGCCC TCAAGGTGTA 
GATGGGCCTC CTGGACCTCC TGGAGGTCCT GGACAACCTG GCTCTCCCGG GTTGCCTGGC 
CAGAAAGGAA GTAAAGGAGA ACCTGGAGTT TCAGGCATTG GTCTCCCTGG TCTTCCTGGT 
CCAAAGGGTG AACCTGGTCT GCCTGGATAT CCAGGAAACC CAGGTATCAA AGGTTCTATG 
GGCGAGGCTG GTCTGCCTGG CTTACCAGGA AGCCCTGGAG CAAAAGGTCA ACCCGGCCTG 
CCCGGATTCC CAGGAACACC AGGGCTTCCT GGACCAAAGG GTATGAATGG TCCTCCTGGT 
AACCCTGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGGCGTCC TGGACCACCA 
GGACCTCCTG GTGAAAAAGG CAAGCCAGGT CAAGATGGCA TTCCTGGACC AGCAGGACAA 
AAGGGAGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CTCCTGGACT CCCAGGACTT 
TCTGGTCAAA AGGGTGATGG AGGAGCCCCT GGCCTTCCAG GAAATCCTGG CCTTCCAGGT 
TCAAAGGGTG AACCAGGCTT TCAGGGATTC CCTGGTATGC CAGGTCCCCC AGGTCCCCCT 
GGTTCTCCTG GCCCATCTTT GGAAGGTCCC AAAGGAAACC CTGGGCCTCA AGGCCCTCCT 
GGGAGACCAG GTCCTCCAGG TTTTCAAGGT CCACCTGGTA CAGAAGGTCC TCGTGGCCCC 
CCTGGAAATG GAGGTATTAA AGGAGAGAGG GGAAATCCAG GTCCACCTGG GCAACCAGGC 
TTACCTGGCT TGAAAGGAGA TCAAGGACCA CCAGGCCTTC CGGGTAACCC TGGCCGACCA 
GGTCTTAATG GAATGAAAGG AGATCCTGGT CTCCCTGGTG TTCCAGGATT CCCAGGAATG 
AAAGGACCCA TTGGAGTACC AGGCTCAACT GGCCCTGATG GGGAACCAGG TCTTACGGGG 
CCCCCAGGTC CTCCTGGGTT GCCTGGTCCT TCAGGACAGA GTATTGTAAT CAAAGGAGAT 
CCTGGACCTC CAGGAATTCC TGGACAACCT GGATTAAAAG GTCTACCAGG ACTTCCAGGC 
CCTCAAGGTC TACCAGGTCC AATTGGCCCT CCAGGAGATC CAGGACGCAA TGGACTCCCA 
GGCTTTGATG GTGCAGGAGG GCGCAAAGGA GACCCAGGCC TGCCGGGGCA GCCAGGTAGC 
CGTGGATTGG ATGGTCCCCC AGGACCAGAT GGATTGCAAG GACCTCCAGG GCCCCCGGGA 
ACCACCTCTG TTGCCCATGG ATTCCTCATC ACACGTCACA GTCAGACAAC AGATGCACCA 
CAATGCCCAC AGGGAACAGT CCATATCTAT GATGGCTTTT CTCTCTTATA TGTACAAGGA 
AATAAAAGAG CCCATGGTCA AGACTTGGGG ACAGCTGGCA GCTGCCTTCG TCGCTTCAGT 
ACCATGCCTT TCATGTTCTG CAACATTAAC AACGTTTGTA ACTTTGCTTC AAGAAATGAC 
TATTCTTACT GGCTTTCCAC CCCAGAGCCC ATGCCAATGA ACATGGAACC CCTGAAGGGC 
CAGAGCATCC AGCCATTCAT TAGTCGATGT GCAGTATGTG AGGCACCAGC TGTGGTGATT 
GCAGTTCACA GCCAGACTAT TCAGATTCCT CACTGTCCTC AGGGGTGGGA CTCTCTGTGG 
ATTGGCTATT CATTCATGAT GCATACAAGT GCAGGAGCAG AAGGCTCTGG CCAAGCCCTA 
GCCTCTCCTG GCTCCTGTTT GGAAGAATTT CGTTCAGCTC CCTTCATCGA ATGTCATGGA 
CGAGGGACAT GCAATTACTA TGCCAATTCT TACAGCTTTT GGCTGGCCAC TGTTGATATG 
TCAGACATGT TCAGTAAACC TCAGTCAGAG ACACTGAAAG CAGGCGACTT GAGGACACGT 
ATTAGCCGAT GTCAAGTATG CATGAAAAGG ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_343779.2
CDS	400..5475
Translation	MQVPGVCLAAGWFLLALCLWGQPGEAAACHGCSSGSKCDCSGIKGEKGERGFPGLEGHPGLPGFPGPEGPPGPRGQKGDDGIPGPPGPKGIRGPPGLPGFPGTPGLPGMPGHDGAPGPQGIPGCNGTKGERGFPGSPGFPGLQGPPGPPGIPGMKGEPGSIIMSSLPGPKGNQGYPGPPGIQGPPGPKGVSGPSGPPGPPGLMGPPGPPGPPGPKGNMGLNFQGPKGEKGEQGLQGPPGPPGQISEQKRPIDLDFQKGDQGVPGDRGPPGPPGIPGPPGPPGGVKGEKGEPGEPGKRGKPGKDGETGQPGIPGLPGDPGYPGEPGRHGEKGQKGDTGPPGPPGFVIPTPGTGVTIGAKGNIGQPGLPGEKGERGFPGIQGPPGFPGPPGTAVMGPPGPPGYPGERGQKGDEGPPGICIPGSPGLDGQPGAPGFPGPPGPPGPQLPPSDEICKAGPPGPPGSPGDQGLQGEQGVKGDKGDTCFNCIGTGISGPPGQPGLPGLPGPPGSLGIPGEKGEKGQAGTTGPKGLPGIPGPPGAPGFPGSKGDPGDVLTLPGMKGEKGELGSPGAPGLPGLPGSPGKDGLPGLPGPKGEPGGITFKGERGPPGNPGLPGLPGNMGPTGPVGFGPPGPVGEKGIQGVAGNPGQPGLPGPKGDPGQTISQPGKPGLPGNPGRDGEVGLPGDPGLPGQPGLPGIPGSKGEPGIPGIGPPGPPGPKGFPGIPGPPGPPGAPGRMGPEGPPGLPGFPGPKGEPGFGLPGPPGPPGFPGFKGTPGPKGDRGLPGPPGPPGHTGLDGLPGPKGDAGPNGQPGPIGPPGLPGIGLQGPPGPPGTPGPIGQPGLHGIPGEKGDPGPPGLDVPGLPGERGSPGLPGAPGLIGPPGSPGIPGRAGVPGFPGVKGEMGMMGPPGPPGPLGTPGRSGAPGLKGDDGMQGQPGLPGPVGEKGGKGEPGLPGPPGPMRPDFLGSKGEKGDPGLPGIPGLSGPKGYQGLPGDPGQPGLSGQPGSPGLPGPKGTPGLPGPPGLTGPPGPKGNIGDMGFPGPQGVDGPPGPPGGPGQPGSPGLPGQKGSKGEPGVSGIGLPGLPGPKGEPGLPGYPGNPGIKGSMGEAGLPGLPGSPGAKGQPGLPGFPGTPGLPGPKGMNGPPGNPGLPGEPGPVGGGGRPGPPGPPGEKGKPGQDGIPGPAGQKGEPGQPGFGIPGPPGLPGLSGQKGDGGAPGLPGNPGLPGSKGEPGFQGFPGMPGPPGPPGSPGPSLEGPKGNPGPQGPPGRPGPPGFQGPPGTEGPRGPPGNGGIKGERGNPGPPGQPGLPGLKGDQGPPGLPGNPGRPGLNGMKGDPGLPGVPGFPGMKGPIGVPGSTGPDGEPGLTGPPGPPGLPGPSGQSIVIKGDPGPPGIPGQPGLKGLPGLPGPQGLPGPIGPPGDPGRNGLPGFDGAGGRKGDPGLPGQPGSRGLDGPPGPDGLQGPPGPPGTTSVAHGFLITRHSQTTDAPQCPQGTVHIYDGFSLLYVQGNKRAHGQDLGTAGSCLRRFSTMPFMFCNINNVCNFASRNDYSYWLSTPEPMPMNMEPLKGQSIQPFISRCAVCEAPAVVIAVHSQTIQIPHCPQGWDSLWIGYSFMMHTSAGAEGSGQALASPGSCLEEFRSAPFIECHGRGTCNYYANSYSFWLATVDMSDMFSKPQSETLKAGDLRTRISRCQVCMKRT

Target ORF information:

RefSeq Version	XM_343778.6
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus collagen type IV alpha 5 chain (Col4a5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_343778.6

ORF Insert Sequence:

ATGCAAGTGC CTGGAGTGTG CCTGGCCGCC GGCTGGTTCT TACTGGCCCT GTGTCTGTGG 
GGGCAGCCGG GAGAGGCTGC GGCCTGCCAT GGGTGTTCTT CTGGATCAAA ATGTGACTGC 
AGTGGTATAA AAGGAGAAAA GGGAGAACGG GGCTTTCCAG GCTTAGAAGG CCATCCAGGT 
TTACCAGGAT TTCCAGGTCC AGAAGGGCCT CCAGGACCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGA ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGCCTTCC TGGGATGCCA GGCCACGATG GGGCTCCAGG ACCTCAAGGT 
ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGCGGATTTC CAGGCAGTCC AGGCTTTCCC 
GGTTTACAAG GACCTCCAGG ACCACCTGGG ATCCCAGGTA TGAAGGGGGA GCCAGGCAGT 
ATAATCATGT CATCTCTGCC AGGGCCAAAG GGTAATCAAG GATACCCAGG TCCTCCTGGA 
ATACAAGGTC CACCTGGTCC CAAGGGTGTA TCAGGGCCAA GTGGTCCTCC AGGACCACCA 
GGTTTAATGG GCCCTCCTGG CCCACCAGGC CCACCGGGAC CAAAGGGGAA TATGGGCTTA 
AATTTCCAGG GACCCAAAGG CGAAAAAGGT GAACAAGGTC TTCAGGGTCC ACCTGGTCCA 
CCTGGGCAGA TCAGTGAACA GAAAAGACCA ATTGACCTAG ATTTTCAGAA AGGCGATCAG 
GGAGTTCCCG GTGATAGAGG GCCTCCTGGA CCTCCGGGAA TACCTGGTCC TCCCGGTCCT 
CCTGGTGGTG TGAAAGGTGA AAAGGGTGAG CCCGGAGAGC CAGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAGACTGG TCAACCAGGA ATCCCAGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGTGAAC CAGGAAGGCA TGGTGAAAAG GGCCAAAAGG GTGATACTGG CCCACCTGGA 
CCACCTGGAT TTGTAATTCC TACACCTGGA ACTGGTGTGA CTATTGGAGC AAAAGGAAAT 
ATTGGGCAAC CTGGTTTGCC TGGAGAAAAA GGAGAGCGAG GATTTCCTGG CATACAAGGT 
CCACCTGGCT TTCCTGGACC TCCAGGGACA GCTGTCATGG GTCCCCCTGG TCCTCCTGGA 
TATCCTGGTG AAAGGGGCCA GAAAGGTGAT GAAGGTCCCC CTGGAATTTG TATTCCTGGA 
TCTCCTGGAC TTGATGGACA GCCTGGAGCT CCTGGCTTTC CAGGACCTCC TGGTCCTCCT 
GGCCCCCAGT TACCCCCGAG TGATGAAATC TGTAAAGCAG GCCCTCCAGG GCCCCCAGGA 
TCTCCAGGTG ACCAAGGACT CCAAGGAGAG CAAGGAGTAA AAGGTGACAA AGGTGATACT 
TGCTTCAACT GTATTGGAAC TGGCATTTCA GGGCCTCCAG GCCAACCAGG TTTACCAGGT 
CTCCCAGGTC CTCCAGGATC TCTTGGAATC CCTGGAGAGA AGGGCGAGAA AGGACAAGCT 
GGGACAACTG GTCCAAAAGG ATTGCCAGGC ATTCCTGGAC CTCCAGGTGC TCCAGGCTTT 
CCAGGGTCTA AGGGTGACCC TGGTGATGTT CTCACTCTTC CGGGAATGAA AGGTGAAAAA 
GGAGAATTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTC TACCTGGTAG TCCTGGAAAG 
GATGGTTTGC CAGGACTTCC TGGCCCCAAA GGAGAGCCCG GTGGAATCAC TTTTAAGGGT 
GAAAGGGGTC CTCCTGGGAA CCCAGGTTTA CCAGGTCTCC CAGGAAATAT GGGACCTACA 
GGCCCTGTTG GTTTTGGGCC TCCAGGCCCC GTAGGTGAAA AAGGCATACA AGGTGTGGCG 
GGAAATCCAG GCCAGCCAGG ATTACCAGGT CCTAAAGGTG ATCCAGGCCA GACCATCAGC 
CAGCCAGGAA AGCCTGGCTT GCCAGGGAAT CCAGGCAGAG ATGGTGAAGT GGGTCTTCCA 
GGTGATCCTG GACTTCCAGG CCAACCAGGC CTACCAGGAA TACCTGGTAG CAAAGGAGAA 
CCAGGTATCC CTGGGATTGG GCCTCCAGGA CCACCTGGTC CCAAAGGTTT TCCAGGAATT 
CCTGGACCTC CAGGACCTCC TGGGGCCCCT GGAAGAATGG GCCCAGAAGG GCCTCCTGGG 
TTACCGGGCT TTCCAGGACC CAAGGGAGAG CCAGGATTTG GGTTACCTGG GCCACCTGGG 
CCACCAGGAT TCCCAGGTTT CAAAGGAACA CCTGGTCCAA AAGGTGATCG TGGTTTACCA 
GGACCTCCAG GTCCTCCAGG ACACACTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAT 
GCTGGACCAA ATGGACAACC TGGCCCAATA GGGCCACCAG GGCTGCCAGG AATTGGTCTT 
CAGGGACCAC CAGGACCACC AGGGACTCCA GGGCCAATAG GCCAACCTGG CTTACATGGA 
ATACCAGGAG AGAAGGGAGA TCCAGGACCT CCTGGGCTTG ATGTTCCAGG ACTCCCTGGA 
GAGAGAGGTA GTCCAGGGCT CCCTGGAGCC CCTGGTCTTA TAGGACCTCC AGGATCACCT 
GGTATCCCTG GAAGAGCAGG TGTCCCTGGA TTTCCAGGTG TCAAAGGCGA AATGGGTATG 
ATGGGACCTC CAGGCCCACC GGGACCTTTG GGTACTCCTG GCAGGAGTGG TGCTCCTGGT 
CTTAAAGGTG ATGACGGCAT GCAAGGTCAG CCAGGTCTCC CTGGCCCTGT AGGAGAAAAG 
GGTGGTAAGG GAGAACCTGG CCTTCCAGGT CCTCCTGGAC CAATGCGTCC AGATTTCCTG 
GGATCAAAAG GAGAAAAGGG TGATCCTGGC TTACCAGGTA TTCCTGGACT TTCCGGACCA 
AAAGGCTACC AGGGATTACC TGGAGACCCA GGGCAACCTG GGCTGAGTGG ACAGCCGGGA 
TCACCAGGAC TACCAGGTCC CAAAGGTACT CCTGGTCTCC CTGGTCCACC AGGACTTACC 
GGACCTCCTG GACCTAAAGG AAACATTGGC GACATGGGTT TTCCTGGCCC TCAAGGTGTA 
GATGGGCCTC CTGGACCTCC TGGAGGTCCT GGACAACCTG GCTCTCCCGG GTTGCCTGGC 
CAGAAAGGAA GTAAAGGAGA ACCTGGAGTT TCAGGCATTG GTCTCCCTGG TCTTCCTGGT 
CCAAAGGGTG AACCTGGTCT GCCTGGATAT CCAGGAAACC CAGGTATCAA AGGTTCTATG 
GGCGAGGCTG GTCTGCCTGG CTTACCAGGA AGCCCTGGAG CAAAAGGTCA ACCCGGCCTG 
CCCGGATTCC CAGGAACACC AGGGCTTCCT GGACCAAAGG GTATGAATGG TCCTCCTGGT 
AACCCTGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGGCGTCC TGGACCACCA 
GGACCTCCTG GTGAAAAAGG CAAGCCAGGT CAAGATGGCA TTCCTGGACC AGCAGGACAA 
AAGGGAGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CTCCTGGACT CCCAGGACTT 
TCTGGTCAAA AGGGTGATGG AGGAGCCCCT GGCCTTCCAG GAAATCCTGG CCTTCCAGGT 
TCAAAGGGTG AACCAGGCTT TCAGGGATTC CCTGGTATGC CAGGTCCCCC AGGTCCCCCT 
GGTTCTCCTG GCCCATCTTT GGAAGGTCCC AAAGGAAACC CTGGGCCTCA AGGCCCTCCT 
GGGAGACCAG GTCCTCCAGG TTTTCAAGGT CCACCTGGTA CAGAAGGTCC TCGTGGCCCC 
CCTGGAAATG GAGGTATTAA AGGAGAGAGG GGAAATCCAG GTCCACCTGG GCAACCAGGC 
TTACCTGGCT TGAAAGGAGA TCAAGGACCA CCAGGCCTTC CGGGTAACCC TGGCCGACCA 
GGTCTTAATG GAATGAAAGG AGATCCTGGT CTCCCTGGTG TTCCAGGATT CCCAGGAATG 
AAAGGACCCA TTGGAGTACC AGGCTCAACT GGCCCTGATG GGGAACCAGG TCTTACGGGG 
CCCCCAGGTC CTCCTGGGTT GCCTGGTCCT TCAGGACAGA GTATTGTAAT CAAAGGAGAT 
CCTGGACCTC CAGGAATTCC TGGACAACCT GGATTAAAAG GTCTACCAGG ACTTCCAGGC 
CCTCAAGGTC TACCAGGTCC AATTGGCCCT CCAGGAGATC CAGGACGCAA TGGACTCCCA 
GGCTTTGATG GTGCAGGAGG GCGCAAAGGA GACCCAGGCC TGCCGGGGCA GCCAGGTAGC 
CGTGGATTGG ATGGTCCCCC AGGACCAGAT GGATTGCAAG GACCTCCAGG GCCCCCGGGA 
ACCACCTCTG TTGCCCATGG ATTCCTCATC ACACGTCACA GTCAGACAAC AGATGCACCA 
CAATGCCCAC AGGGAACAGT CCATATCTAT GATGGCTTTT CTCTCTTATA TGTACAAGGA 
AATAAAAGAG CCCATGGTCA AGACTTGGGG ACAGCTGGCA GCTGCCTTCG TCGCTTCAGT 
ACCATGCCTT TCATGTTCTG CAACATTAAC AACGTTTGTA ACTTTGCTTC AAGAAATGAC 
TATTCTTACT GGCTTTCCAC CCCAGAGCCC ATGCCAATGA ACATGGAACC CCTGAAGGGC 
CAGAGCATCC AGCCATTCAT TAGTCGATGT GCAGTATGTG AGGCACCAGC TGTGGTGATT 
GCAGTTCACA GCCAGACTAT TCAGATTCCT CACTGTCCTC AGGGGTGGGA CTCTCTGTGG 
ATTGGCTATT CATTCATGAT GCATACAAGT GCAGGAGCAG AAGGCTCTGG CCAAGCCCTA 
GCCTCTCCTG GCTCCTGTTT GGAAGAATTT CGTTCAGCTC CCTTCATCGA ATGTCATGGA 
CGAGGGACAT GCAATTACTA TGCCAATTCT TACAGCTTTT GGCTGGCCAC TGTTGATATG 
TCAGACATGT TCAGTAAACC TCAGTCAGAG ACACTGAAAG CAGGCGACTT GAGGACACGT 
ATTAGCCGAT GTCAAGTATG CATGAAAAGG ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col4a5 ( XM_001055156.4 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_001055156.1 Rattus norvegicus collagen type IV alpha 5 chain (Col4a5), transcript variant X1, mRNA.

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CloneID	ORa21617
Clone ID Related Accession (Same CDS sequence)	XM_343778.6 , XM_001055156.4
Accession Version	XM_001055156.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5076bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	collagen alpha-5(IV) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000089.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 9, 2013 this sequence version replaced XM_001055156.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGTGC CTGGAGTGTG CCTGGCCGCC GGCTGGTTCT TACTGGCCCT GTGTCTGTGG 
GGGCAGCCGG GAGAGGCTGC GGCCTGCCAT GGGTGTTCTT CTGGATCAAA ATGTGACTGC 
AGTGGTATAA AAGGAGAAAA GGGAGAACGG GGCTTTCCAG GCTTAGAAGG CCATCCAGGT 
TTACCAGGAT TTCCAGGTCC AGAAGGGCCT CCAGGACCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGA ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGCCTTCC TGGGATGCCA GGCCACGATG GGGCTCCAGG ACCTCAAGGT 
ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGCGGATTTC CAGGCAGTCC AGGCTTTCCC 
GGTTTACAAG GACCTCCAGG ACCACCTGGG ATCCCAGGTA TGAAGGGGGA GCCAGGCAGT 
ATAATCATGT CATCTCTGCC AGGGCCAAAG GGTAATCAAG GATACCCAGG TCCTCCTGGA 
ATACAAGGTC CACCTGGTCC CAAGGGTGTA TCAGGGCCAA GTGGTCCTCC AGGACCACCA 
GGTTTAATGG GCCCTCCTGG CCCACCAGGC CCACCGGGAC CAAAGGGGAA TATGGGCTTA 
AATTTCCAGG GACCCAAAGG CGAAAAAGGT GAACAAGGTC TTCAGGGTCC ACCTGGTCCA 
CCTGGGCAGA TCAGTGAACA GAAAAGACCA ATTGACCTAG ATTTTCAGAA AGGCGATCAG 
GGAGTTCCCG GTGATAGAGG GCCTCCTGGA CCTCCGGGAA TACCTGGTCC TCCCGGTCCT 
CCTGGTGGTG TGAAAGGTGA AAAGGGTGAG CCCGGAGAGC CAGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAGACTGG TCAACCAGGA ATCCCAGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGTGAAC CAGGAAGGCA TGGTGAAAAG GGCCAAAAGG GTGATACTGG CCCACCTGGA 
CCACCTGGAT TTGTAATTCC TACACCTGGA ACTGGTGTGA CTATTGGAGC AAAAGGAAAT 
ATTGGGCAAC CTGGTTTGCC TGGAGAAAAA GGAGAGCGAG GATTTCCTGG CATACAAGGT 
CCACCTGGCT TTCCTGGACC TCCAGGGACA GCTGTCATGG GTCCCCCTGG TCCTCCTGGA 
TATCCTGGTG AAAGGGGCCA GAAAGGTGAT GAAGGTCCCC CTGGAATTTG TATTCCTGGA 
TCTCCTGGAC TTGATGGACA GCCTGGAGCT CCTGGCTTTC CAGGACCTCC TGGTCCTCCT 
GGCCCCCAGT TACCCCCGAG TGATGAAATC TGTAAAGCAG GCCCTCCAGG GCCCCCAGGA 
TCTCCAGGTG ACCAAGGACT CCAAGGAGAG CAAGGAGTAA AAGGTGACAA AGGTGATACT 
TGCTTCAACT GTATTGGAAC TGGCATTTCA GGGCCTCCAG GCCAACCAGG TTTACCAGGT 
CTCCCAGGTC CTCCAGGATC TCTTGGAATC CCTGGAGAGA AGGGCGAGAA AGGACAAGCT 
GGGACAACTG GTCCAAAAGG ATTGCCAGGC ATTCCTGGAC CTCCAGGTGC TCCAGGCTTT 
CCAGGGTCTA AGGGTGACCC TGGTGATGTT CTCACTCTTC CGGGAATGAA AGGTGAAAAA 
GGAGAATTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTC TACCTGGTAG TCCTGGAAAG 
GATGGTTTGC CAGGACTTCC TGGCCCCAAA GGAGAGCCCG GTGGAATCAC TTTTAAGGGT 
GAAAGGGGTC CTCCTGGGAA CCCAGGTTTA CCAGGTCTCC CAGGAAATAT GGGACCTACA 
GGCCCTGTTG GTTTTGGGCC TCCAGGCCCC GTAGGTGAAA AAGGCATACA AGGTGTGGCG 
GGAAATCCAG GCCAGCCAGG ATTACCAGGT CCTAAAGGTG ATCCAGGCCA GACCATCAGC 
CAGCCAGGAA AGCCTGGCTT GCCAGGGAAT CCAGGCAGAG ATGGTGAAGT GGGTCTTCCA 
GGTGATCCTG GACTTCCAGG CCAACCAGGC CTACCAGGAA TACCTGGTAG CAAAGGAGAA 
CCAGGTATCC CTGGGATTGG GCCTCCAGGA CCACCTGGTC CCAAAGGTTT TCCAGGAATT 
CCTGGACCTC CAGGACCTCC TGGGGCCCCT GGAAGAATGG GCCCAGAAGG GCCTCCTGGG 
TTACCGGGCT TTCCAGGACC CAAGGGAGAG CCAGGATTTG GGTTACCTGG GCCACCTGGG 
CCACCAGGAT TCCCAGGTTT CAAAGGAACA CCTGGTCCAA AAGGTGATCG TGGTTTACCA 
GGACCTCCAG GTCCTCCAGG ACACACTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAT 
GCTGGACCAA ATGGACAACC TGGCCCAATA GGGCCACCAG GGCTGCCAGG AATTGGTCTT 
CAGGGACCAC CAGGACCACC AGGGACTCCA GGGCCAATAG GCCAACCTGG CTTACATGGA 
ATACCAGGAG AGAAGGGAGA TCCAGGACCT CCTGGGCTTG ATGTTCCAGG ACTCCCTGGA 
GAGAGAGGTA GTCCAGGGCT CCCTGGAGCC CCTGGTCTTA TAGGACCTCC AGGATCACCT 
GGTATCCCTG GAAGAGCAGG TGTCCCTGGA TTTCCAGGTG TCAAAGGCGA AATGGGTATG 
ATGGGACCTC CAGGCCCACC GGGACCTTTG GGTACTCCTG GCAGGAGTGG TGCTCCTGGT 
CTTAAAGGTG ATGACGGCAT GCAAGGTCAG CCAGGTCTCC CTGGCCCTGT AGGAGAAAAG 
GGTGGTAAGG GAGAACCTGG CCTTCCAGGT CCTCCTGGAC CAATGCGTCC AGATTTCCTG 
GGATCAAAAG GAGAAAAGGG TGATCCTGGC TTACCAGGTA TTCCTGGACT TTCCGGACCA 
AAAGGCTACC AGGGATTACC TGGAGACCCA GGGCAACCTG GGCTGAGTGG ACAGCCGGGA 
TCACCAGGAC TACCAGGTCC CAAAGGTACT CCTGGTCTCC CTGGTCCACC AGGACTTACC 
GGACCTCCTG GACCTAAAGG AAACATTGGC GACATGGGTT TTCCTGGCCC TCAAGGTGTA 
GATGGGCCTC CTGGACCTCC TGGAGGTCCT GGACAACCTG GCTCTCCCGG GTTGCCTGGC 
CAGAAAGGAA GTAAAGGAGA ACCTGGAGTT TCAGGCATTG GTCTCCCTGG TCTTCCTGGT 
CCAAAGGGTG AACCTGGTCT GCCTGGATAT CCAGGAAACC CAGGTATCAA AGGTTCTATG 
GGCGAGGCTG GTCTGCCTGG CTTACCAGGA AGCCCTGGAG CAAAAGGTCA ACCCGGCCTG 
CCCGGATTCC CAGGAACACC AGGGCTTCCT GGACCAAAGG GTATGAATGG TCCTCCTGGT 
AACCCTGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGGCGTCC TGGACCACCA 
GGACCTCCTG GTGAAAAAGG CAAGCCAGGT CAAGATGGCA TTCCTGGACC AGCAGGACAA 
AAGGGAGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CTCCTGGACT CCCAGGACTT 
TCTGGTCAAA AGGGTGATGG AGGAGCCCCT GGCCTTCCAG GAAATCCTGG CCTTCCAGGT 
TCAAAGGGTG AACCAGGCTT TCAGGGATTC CCTGGTATGC CAGGTCCCCC AGGTCCCCCT 
GGTTCTCCTG GCCCATCTTT GGAAGGTCCC AAAGGAAACC CTGGGCCTCA AGGCCCTCCT 
GGGAGACCAG GTCCTCCAGG TTTTCAAGGT CCACCTGGTA CAGAAGGTCC TCGTGGCCCC 
CCTGGAAATG GAGGTATTAA AGGAGAGAGG GGAAATCCAG GTCCACCTGG GCAACCAGGC 
TTACCTGGCT TGAAAGGAGA TCAAGGACCA CCAGGCCTTC CGGGTAACCC TGGCCGACCA 
GGTCTTAATG GAATGAAAGG AGATCCTGGT CTCCCTGGTG TTCCAGGATT CCCAGGAATG 
AAAGGACCCA TTGGAGTACC AGGCTCAACT GGCCCTGATG GGGAACCAGG TCTTACGGGG 
CCCCCAGGTC CTCCTGGGTT GCCTGGTCCT TCAGGACAGA GTATTGTAAT CAAAGGAGAT 
CCTGGACCTC CAGGAATTCC TGGACAACCT GGATTAAAAG GTCTACCAGG ACTTCCAGGC 
CCTCAAGGTC TACCAGGTCC AATTGGCCCT CCAGGAGATC CAGGACGCAA TGGACTCCCA 
GGCTTTGATG GTGCAGGAGG GCGCAAAGGA GACCCAGGCC TGCCGGGGCA GCCAGGTAGC 
CGTGGATTGG ATGGTCCCCC AGGACCAGAT GGATTGCAAG GACCTCCAGG GCCCCCGGGA 
ACCACCTCTG TTGCCCATGG ATTCCTCATC ACACGTCACA GTCAGACAAC AGATGCACCA 
CAATGCCCAC AGGGAACAGT CCATATCTAT GATGGCTTTT CTCTCTTATA TGTACAAGGA 
AATAAAAGAG CCCATGGTCA AGACTTGGGG ACAGCTGGCA GCTGCCTTCG TCGCTTCAGT 
ACCATGCCTT TCATGTTCTG CAACATTAAC AACGTTTGTA ACTTTGCTTC AAGAAATGAC 
TATTCTTACT GGCTTTCCAC CCCAGAGCCC ATGCCAATGA ACATGGAACC CCTGAAGGGC 
CAGAGCATCC AGCCATTCAT TAGTCGATGT GCAGTATGTG AGGCACCAGC TGTGGTGATT 
GCAGTTCACA GCCAGACTAT TCAGATTCCT CACTGTCCTC AGGGGTGGGA CTCTCTGTGG 
ATTGGCTATT CATTCATGAT GCATACAAGT GCAGGAGCAG AAGGCTCTGG CCAAGCCCTA 
GCCTCTCCTG GCTCCTGTTT GGAAGAATTT CGTTCAGCTC CCTTCATCGA ATGTCATGGA 
CGAGGGACAT GCAATTACTA TGCCAATTCT TACAGCTTTT GGCTGGCCAC TGTTGATATG 
TCAGACATGT TCAGTAAACC TCAGTCAGAG ACACTGAAAG CAGGCGACTT GAGGACACGT 
ATTAGCCGAT GTCAAGTATG CATGAAAAGG ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001055156.1
CDS	400..5475
Translation	MQVPGVCLAAGWFLLALCLWGQPGEAAACHGCSSGSKCDCSGIKGEKGERGFPGLEGHPGLPGFPGPEGPPGPRGQKGDDGIPGPPGPKGIRGPPGLPGFPGTPGLPGMPGHDGAPGPQGIPGCNGTKGERGFPGSPGFPGLQGPPGPPGIPGMKGEPGSIIMSSLPGPKGNQGYPGPPGIQGPPGPKGVSGPSGPPGPPGLMGPPGPPGPPGPKGNMGLNFQGPKGEKGEQGLQGPPGPPGQISEQKRPIDLDFQKGDQGVPGDRGPPGPPGIPGPPGPPGGVKGEKGEPGEPGKRGKPGKDGETGQPGIPGLPGDPGYPGEPGRHGEKGQKGDTGPPGPPGFVIPTPGTGVTIGAKGNIGQPGLPGEKGERGFPGIQGPPGFPGPPGTAVMGPPGPPGYPGERGQKGDEGPPGICIPGSPGLDGQPGAPGFPGPPGPPGPQLPPSDEICKAGPPGPPGSPGDQGLQGEQGVKGDKGDTCFNCIGTGISGPPGQPGLPGLPGPPGSLGIPGEKGEKGQAGTTGPKGLPGIPGPPGAPGFPGSKGDPGDVLTLPGMKGEKGELGSPGAPGLPGLPGSPGKDGLPGLPGPKGEPGGITFKGERGPPGNPGLPGLPGNMGPTGPVGFGPPGPVGEKGIQGVAGNPGQPGLPGPKGDPGQTISQPGKPGLPGNPGRDGEVGLPGDPGLPGQPGLPGIPGSKGEPGIPGIGPPGPPGPKGFPGIPGPPGPPGAPGRMGPEGPPGLPGFPGPKGEPGFGLPGPPGPPGFPGFKGTPGPKGDRGLPGPPGPPGHTGLDGLPGPKGDAGPNGQPGPIGPPGLPGIGLQGPPGPPGTPGPIGQPGLHGIPGEKGDPGPPGLDVPGLPGERGSPGLPGAPGLIGPPGSPGIPGRAGVPGFPGVKGEMGMMGPPGPPGPLGTPGRSGAPGLKGDDGMQGQPGLPGPVGEKGGKGEPGLPGPPGPMRPDFLGSKGEKGDPGLPGIPGLSGPKGYQGLPGDPGQPGLSGQPGSPGLPGPKGTPGLPGPPGLTGPPGPKGNIGDMGFPGPQGVDGPPGPPGGPGQPGSPGLPGQKGSKGEPGVSGIGLPGLPGPKGEPGLPGYPGNPGIKGSMGEAGLPGLPGSPGAKGQPGLPGFPGTPGLPGPKGMNGPPGNPGLPGEPGPVGGGGRPGPPGPPGEKGKPGQDGIPGPAGQKGEPGQPGFGIPGPPGLPGLSGQKGDGGAPGLPGNPGLPGSKGEPGFQGFPGMPGPPGPPGSPGPSLEGPKGNPGPQGPPGRPGPPGFQGPPGTEGPRGPPGNGGIKGERGNPGPPGQPGLPGLKGDQGPPGLPGNPGRPGLNGMKGDPGLPGVPGFPGMKGPIGVPGSTGPDGEPGLTGPPGPPGLPGPSGQSIVIKGDPGPPGIPGQPGLKGLPGLPGPQGLPGPIGPPGDPGRNGLPGFDGAGGRKGDPGLPGQPGSRGLDGPPGPDGLQGPPGPPGTTSVAHGFLITRHSQTTDAPQCPQGTVHIYDGFSLLYVQGNKRAHGQDLGTAGSCLRRFSTMPFMFCNINNVCNFASRNDYSYWLSTPEPMPMNMEPLKGQSIQPFISRCAVCEAPAVVIAVHSQTIQIPHCPQGWDSLWIGYSFMMHTSAGAEGSGQALASPGSCLEEFRSAPFIECHGRGTCNYYANSYSFWLATVDMSDMFSKPQSETLKAGDLRTRISRCQVCMKRT

Target ORF information:

RefSeq Version	XM_001055156.4
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus collagen type IV alpha 5 chain (Col4a5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001055156.4

ORF Insert Sequence:

ATGCAAGTGC CTGGAGTGTG CCTGGCCGCC GGCTGGTTCT TACTGGCCCT GTGTCTGTGG 
GGGCAGCCGG GAGAGGCTGC GGCCTGCCAT GGGTGTTCTT CTGGATCAAA ATGTGACTGC 
AGTGGTATAA AAGGAGAAAA GGGAGAACGG GGCTTTCCAG GCTTAGAAGG CCATCCAGGT 
TTACCAGGAT TTCCAGGTCC AGAAGGGCCT CCAGGACCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGA ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGCCTTCC TGGGATGCCA GGCCACGATG GGGCTCCAGG ACCTCAAGGT 
ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGCGGATTTC CAGGCAGTCC AGGCTTTCCC 
GGTTTACAAG GACCTCCAGG ACCACCTGGG ATCCCAGGTA TGAAGGGGGA GCCAGGCAGT 
ATAATCATGT CATCTCTGCC AGGGCCAAAG GGTAATCAAG GATACCCAGG TCCTCCTGGA 
ATACAAGGTC CACCTGGTCC CAAGGGTGTA TCAGGGCCAA GTGGTCCTCC AGGACCACCA 
GGTTTAATGG GCCCTCCTGG CCCACCAGGC CCACCGGGAC CAAAGGGGAA TATGGGCTTA 
AATTTCCAGG GACCCAAAGG CGAAAAAGGT GAACAAGGTC TTCAGGGTCC ACCTGGTCCA 
CCTGGGCAGA TCAGTGAACA GAAAAGACCA ATTGACCTAG ATTTTCAGAA AGGCGATCAG 
GGAGTTCCCG GTGATAGAGG GCCTCCTGGA CCTCCGGGAA TACCTGGTCC TCCCGGTCCT 
CCTGGTGGTG TGAAAGGTGA AAAGGGTGAG CCCGGAGAGC CAGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAGACTGG TCAACCAGGA ATCCCAGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGTGAAC CAGGAAGGCA TGGTGAAAAG GGCCAAAAGG GTGATACTGG CCCACCTGGA 
CCACCTGGAT TTGTAATTCC TACACCTGGA ACTGGTGTGA CTATTGGAGC AAAAGGAAAT 
ATTGGGCAAC CTGGTTTGCC TGGAGAAAAA GGAGAGCGAG GATTTCCTGG CATACAAGGT 
CCACCTGGCT TTCCTGGACC TCCAGGGACA GCTGTCATGG GTCCCCCTGG TCCTCCTGGA 
TATCCTGGTG AAAGGGGCCA GAAAGGTGAT GAAGGTCCCC CTGGAATTTG TATTCCTGGA 
TCTCCTGGAC TTGATGGACA GCCTGGAGCT CCTGGCTTTC CAGGACCTCC TGGTCCTCCT 
GGCCCCCAGT TACCCCCGAG TGATGAAATC TGTAAAGCAG GCCCTCCAGG GCCCCCAGGA 
TCTCCAGGTG ACCAAGGACT CCAAGGAGAG CAAGGAGTAA AAGGTGACAA AGGTGATACT 
TGCTTCAACT GTATTGGAAC TGGCATTTCA GGGCCTCCAG GCCAACCAGG TTTACCAGGT 
CTCCCAGGTC CTCCAGGATC TCTTGGAATC CCTGGAGAGA AGGGCGAGAA AGGACAAGCT 
GGGACAACTG GTCCAAAAGG ATTGCCAGGC ATTCCTGGAC CTCCAGGTGC TCCAGGCTTT 
CCAGGGTCTA AGGGTGACCC TGGTGATGTT CTCACTCTTC CGGGAATGAA AGGTGAAAAA 
GGAGAATTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTC TACCTGGTAG TCCTGGAAAG 
GATGGTTTGC CAGGACTTCC TGGCCCCAAA GGAGAGCCCG GTGGAATCAC TTTTAAGGGT 
GAAAGGGGTC CTCCTGGGAA CCCAGGTTTA CCAGGTCTCC CAGGAAATAT GGGACCTACA 
GGCCCTGTTG GTTTTGGGCC TCCAGGCCCC GTAGGTGAAA AAGGCATACA AGGTGTGGCG 
GGAAATCCAG GCCAGCCAGG ATTACCAGGT CCTAAAGGTG ATCCAGGCCA GACCATCAGC 
CAGCCAGGAA AGCCTGGCTT GCCAGGGAAT CCAGGCAGAG ATGGTGAAGT GGGTCTTCCA 
GGTGATCCTG GACTTCCAGG CCAACCAGGC CTACCAGGAA TACCTGGTAG CAAAGGAGAA 
CCAGGTATCC CTGGGATTGG GCCTCCAGGA CCACCTGGTC CCAAAGGTTT TCCAGGAATT 
CCTGGACCTC CAGGACCTCC TGGGGCCCCT GGAAGAATGG GCCCAGAAGG GCCTCCTGGG 
TTACCGGGCT TTCCAGGACC CAAGGGAGAG CCAGGATTTG GGTTACCTGG GCCACCTGGG 
CCACCAGGAT TCCCAGGTTT CAAAGGAACA CCTGGTCCAA AAGGTGATCG TGGTTTACCA 
GGACCTCCAG GTCCTCCAGG ACACACTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAT 
GCTGGACCAA ATGGACAACC TGGCCCAATA GGGCCACCAG GGCTGCCAGG AATTGGTCTT 
CAGGGACCAC CAGGACCACC AGGGACTCCA GGGCCAATAG GCCAACCTGG CTTACATGGA 
ATACCAGGAG AGAAGGGAGA TCCAGGACCT CCTGGGCTTG ATGTTCCAGG ACTCCCTGGA 
GAGAGAGGTA GTCCAGGGCT CCCTGGAGCC CCTGGTCTTA TAGGACCTCC AGGATCACCT 
GGTATCCCTG GAAGAGCAGG TGTCCCTGGA TTTCCAGGTG TCAAAGGCGA AATGGGTATG 
ATGGGACCTC CAGGCCCACC GGGACCTTTG GGTACTCCTG GCAGGAGTGG TGCTCCTGGT 
CTTAAAGGTG ATGACGGCAT GCAAGGTCAG CCAGGTCTCC CTGGCCCTGT AGGAGAAAAG 
GGTGGTAAGG GAGAACCTGG CCTTCCAGGT CCTCCTGGAC CAATGCGTCC AGATTTCCTG 
GGATCAAAAG GAGAAAAGGG TGATCCTGGC TTACCAGGTA TTCCTGGACT TTCCGGACCA 
AAAGGCTACC AGGGATTACC TGGAGACCCA GGGCAACCTG GGCTGAGTGG ACAGCCGGGA 
TCACCAGGAC TACCAGGTCC CAAAGGTACT CCTGGTCTCC CTGGTCCACC AGGACTTACC 
GGACCTCCTG GACCTAAAGG AAACATTGGC GACATGGGTT TTCCTGGCCC TCAAGGTGTA 
GATGGGCCTC CTGGACCTCC TGGAGGTCCT GGACAACCTG GCTCTCCCGG GTTGCCTGGC 
CAGAAAGGAA GTAAAGGAGA ACCTGGAGTT TCAGGCATTG GTCTCCCTGG TCTTCCTGGT 
CCAAAGGGTG AACCTGGTCT GCCTGGATAT CCAGGAAACC CAGGTATCAA AGGTTCTATG 
GGCGAGGCTG GTCTGCCTGG CTTACCAGGA AGCCCTGGAG CAAAAGGTCA ACCCGGCCTG 
CCCGGATTCC CAGGAACACC AGGGCTTCCT GGACCAAAGG GTATGAATGG TCCTCCTGGT 
AACCCTGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGGCGTCC TGGACCACCA 
GGACCTCCTG GTGAAAAAGG CAAGCCAGGT CAAGATGGCA TTCCTGGACC AGCAGGACAA 
AAGGGAGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CTCCTGGACT CCCAGGACTT 
TCTGGTCAAA AGGGTGATGG AGGAGCCCCT GGCCTTCCAG GAAATCCTGG CCTTCCAGGT 
TCAAAGGGTG AACCAGGCTT TCAGGGATTC CCTGGTATGC CAGGTCCCCC AGGTCCCCCT 
GGTTCTCCTG GCCCATCTTT GGAAGGTCCC AAAGGAAACC CTGGGCCTCA AGGCCCTCCT 
GGGAGACCAG GTCCTCCAGG TTTTCAAGGT CCACCTGGTA CAGAAGGTCC TCGTGGCCCC 
CCTGGAAATG GAGGTATTAA AGGAGAGAGG GGAAATCCAG GTCCACCTGG GCAACCAGGC 
TTACCTGGCT TGAAAGGAGA TCAAGGACCA CCAGGCCTTC CGGGTAACCC TGGCCGACCA 
GGTCTTAATG GAATGAAAGG AGATCCTGGT CTCCCTGGTG TTCCAGGATT CCCAGGAATG 
AAAGGACCCA TTGGAGTACC AGGCTCAACT GGCCCTGATG GGGAACCAGG TCTTACGGGG 
CCCCCAGGTC CTCCTGGGTT GCCTGGTCCT TCAGGACAGA GTATTGTAAT CAAAGGAGAT 
CCTGGACCTC CAGGAATTCC TGGACAACCT GGATTAAAAG GTCTACCAGG ACTTCCAGGC 
CCTCAAGGTC TACCAGGTCC AATTGGCCCT CCAGGAGATC CAGGACGCAA TGGACTCCCA 
GGCTTTGATG GTGCAGGAGG GCGCAAAGGA GACCCAGGCC TGCCGGGGCA GCCAGGTAGC 
CGTGGATTGG ATGGTCCCCC AGGACCAGAT GGATTGCAAG GACCTCCAGG GCCCCCGGGA 
ACCACCTCTG TTGCCCATGG ATTCCTCATC ACACGTCACA GTCAGACAAC AGATGCACCA 
CAATGCCCAC AGGGAACAGT CCATATCTAT GATGGCTTTT CTCTCTTATA TGTACAAGGA 
AATAAAAGAG CCCATGGTCA AGACTTGGGG ACAGCTGGCA GCTGCCTTCG TCGCTTCAGT 
ACCATGCCTT TCATGTTCTG CAACATTAAC AACGTTTGTA ACTTTGCTTC AAGAAATGAC 
TATTCTTACT GGCTTTCCAC CCCAGAGCCC ATGCCAATGA ACATGGAACC CCTGAAGGGC 
CAGAGCATCC AGCCATTCAT TAGTCGATGT GCAGTATGTG AGGCACCAGC TGTGGTGATT 
GCAGTTCACA GCCAGACTAT TCAGATTCCT CACTGTCCTC AGGGGTGGGA CTCTCTGTGG 
ATTGGCTATT CATTCATGAT GCATACAAGT GCAGGAGCAG AAGGCTCTGG CCAAGCCCTA 
GCCTCTCCTG GCTCCTGTTT GGAAGAATTT CGTTCAGCTC CCTTCATCGA ATGTCATGGA 
CGAGGGACAT GCAATTACTA TGCCAATTCT TACAGCTTTT GGCTGGCCAC TGTTGATATG 
TCAGACATGT TCAGTAAACC TCAGTCAGAG ACACTGAAAG CAGGCGACTT GAGGACACGT 
ATTAGCCGAT GTCAAGTATG CATGAAAAGG ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col4a5 ( XM_006227490.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006227552.1 Rattus norvegicus collagen type IV alpha 5 chain (Col4a5), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa21618
Clone ID Related Accession (Same CDS sequence)	XM_006227490.1 , XM_006257312.1
Accession Version	XM_006227490.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5067bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	collagen alpha-5(IV) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000089.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGTGC CTGGAGTGTG CCTGGCCGCC GGCTGGTTCT TACTGGCCCT GTGTCTGTGG 
GGGCAGCCGG GAGAGGCTGC GGCCTGCCAT GGGTGTTCTT CTGGATCAAA ATGTGACTGC 
AGTGGTATAA AAGGAGAAAA GGGAGAACGG GGCTTTCCAG GCTTAGAAGG CCATCCAGGT 
TTACCAGGAT TTCCAGGTCC AGAAGGGCCT CCAGGACCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGA ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGCCTTCC TGGGATGCCA GGCCACGATG GGGCTCCAGG ACCTCAAGGT 
ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGCGGATTTC CAGGCAGTCC AGGCTTTCCC 
GGTTTACAAG GACCTCCAGG ACCACCTGGG ATCCCAGGTA TGAAGGGGGA GCCAGGCAGT 
ATAATCATGT CATCTCTGCC AGGGCCAAAG GGTAATCAAG GATACCCAGG TCCTCCTGGA 
ATACAAGGTC CACCTGGTCC CAAGGGTGTA TCAGGGCCAA GTGGTCCTCC AGGACCACCA 
GGTTTAATGG GCCCTCCTGG CCCACCAGGC CCACCGGGAC CAAAGGGGAA TATGGGCTTA 
AATTTCCAGG GACCCAAAGG CGAAAAAGGT GAACAAGGTC TTCAGGGTCC ACCTGGTCCA 
CCTGGGCAGA TCAGTGAACA GAAAAGACCA ATTGACCTAG ATTTTCAGAA AGGCGATCAG 
GGAGTTCCCG GTGATAGAGG GCCTCCTGGA CCTCCGGGAA TACCTGGTCC TCCCGGTCCT 
CCTGGTGGTG TGAAAGGTGA AAAGGGTGAG CCCGGAGAGC CAGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAGACTGG TCAACCAGGA ATCCCAGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGTGAAC CAGGAAGGCA TGGTGAAAAG GGCCAAAAGG GTGATACTGG CCCACCTGGA 
CCACCTGGAT TTGTAATTCC TACACCTGGA ACTGGTGTGA CTATTGGAGC AAAAGGAAAT 
ATTGGGCAAC CTGGTTTGCC TGGAGAAAAA GGAGAGCGAG GATTTCCTGG CATACAAGGT 
CCACCTGGCT TTCCTGGACC TCCAGGGACA GCTGTCATGG GTCCCCCTGG TCCTCCTGGA 
TATCCTGGTG AAAGGGGCCA GAAAGGTGAT GAAGGTCCCC CTGGAATTTG TATTCCTGGA 
TCTCCTGGAC TTGATGGACA GCCTGGAGCT CCTGGCTTTC CAGGACCTCC TGGTCCTCCT 
GGCCCCCAGT TACCCCCGAG TGATGAAATC TGTAAAGCAG GCCCTCCAGG GCCCCCAGGA 
TCTCCAGGTG ACCAAGGACT CCAAGGAGAG CAAGGAGTAA AAGGTGACAA AGGTGATACT 
TGCTTCAACT GTATTGGAAC TGGCATTTCA GGGCCTCCAG GCCAACCAGG TTTACCAGGT 
CTCCCAGGTC CTCCAGGATC TCTTGGAATC CCTGGAGAGA AGGGCGAGAA AGGACAAGCT 
GGGACAACTG GTCCAAAAGG ATTGCCAGGC ATTCCTGGAC CTCCAGGTGC TCCAGGCTTT 
CCAGGGTCTA AGGGTGACCC TGGTGATGTT CTCACTCTTC CGGGAATGAA AGGTGAAAAA 
GGAGAATTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTC TACCTGGTAG TCCTGGAAAG 
GATGGTTTGC CAGGACTTCC TGGCCCCAAA GGAGAGCCCG GTGGAATCAC TTTTAAGGGT 
GAAAGGGGTC CTCCTGGGAA CCCAGGTTTA CCAGGTCTCC CAGGAAATAT GGGACCTACA 
GGCCCTGTTG GTTTTGGGCC TCCAGGCCCC GTAGGTGAAA AAGGCATACA AGGTGTGGCG 
GGAAATCCAG GCCAGCCAGG ATTACCAGGT CCTAAAGGTG ATCCAGGCCA GACCATCAGC 
CAGCCAGGAA AGCCTGGCTT GCCAGGGAAT CCAGGCAGAG ATGGTGAAGT GGGTCTTCCA 
GGTGATCCTG GACTTCCAGG CCAACCAGGC CTACCAGGAA TACCTGGTAG CAAAGGAGAA 
CCAGGTATCC CTGGGATTGG GCCTCCAGGA CCACCTGGTC CCAAAGGTTT TCCAGGAATT 
CCTGGACCTC CAGGACCTCC TGGGGCCCCT GGAAGAATGG GCCCAGAAGG GCCTCCTGGG 
TTACCGGGCT TTCCAGGACC CAAGGGAGAG CCAGGATTTG GGTTACCTGG GCCACCTGGG 
CCACCAGGAT TCCCAGGTTT CAAAGGAACA CCTGGTCCAA AAGGTGATCG TGGTTTACCA 
GGACCTCCAG GTCCTCCAGG ACACACTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAT 
GCTGGACCAA ATGGACAACC TGGCCCAATA GGGCCACCAG GGCTGCCAGG AATTGGTCTT 
CAGGGACCAC CAGGACCACC AGGGACTCCA GGGCCAATAG GCCAACCTGG CTTACATGGA 
ATACCAGGAG AGAAGGGAGA TCCAGGACCT CCTGGGCTTG ATGTTCCAGG ACTCCCTGGA 
GAGAGAGGTA GTCCAGGGCT CCCTGGAGCC CCTGGTCTTA TAGGACCTCC AGGATCACCT 
GGTATCCCTG GAAGAGCAGG TGTCCCTGGA TTTCCAGGTG TCAAAGGCGA AATGGGTATG 
ATGGGACCTC CAGGCCCACC GGGACCTTTG GGTACTCCTG GCAGGAGTGG TGCTCCTGGT 
CTTAAAGGTG ATGACGGCAT GCAAGGTCAG CCAGGTCTCC CTGGCCCTGT AGGAGAAAAG 
GGTGGTAAGG GAGAACCTGG CCTTCCAGGT CCTCCTGGAC CAATGCGTCC AGATTTCCTG 
GGATCAAAAG GAGAAAAGGG TGATCCTGGC TTACCAGGTA TTCCTGGACT TTCCGGACCA 
AAAGGCTACC AGGGATTACC TGGAGACCCA GGGCAACCTG GGCTGAGTGG ACAGCCGGGA 
TCACCAGGAC TACCAGGTCC CAAAGGTACT CCTGGTCTCC CTGGTCCACC AGGACTTACC 
GGACCTCCTG GACCTAAAGG AAACATTGGC GACATGGGTT TTCCTGGCCC TCAAGGTGTA 
GATGGGCCTC CTGGACCTCC TGGAGGTCCT GGACAACCTG GCTCTCCCGG GTTGCCTGGC 
CAGAAAGGAA GTAAAGGAGA ACCTGGAGTT TCAGGCATTG GTCTCCCTGG TCTTCCTGGT 
CCAAAGGGTG AACCTGGTCT GCCTGGATAT CCAGGAAACC CAGGTATCAA AGGTTCTATG 
GGCGAGGCTG GTCTGCCTGG CTTACCAGGA AGCCCTGGAG CAAAAGGTCA ACCCGGCCTG 
CCCGGATTCC CAGGAACACC AGGGCTTCCT GGACCAAAGG GTATGAATGG TCCTCCTGGT 
AACCCTGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGGCGTCC TGGACCACCA 
GGACCTCCTG GTGAAAAAGG CAAGCCAGGT CAAGATGGCA TTCCTGGACC AGCAGGACAA 
AAGGGAGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CTCCTGGACT CCCAGGACTT 
TCTGGTCAAA AGGGTGATGG AGGAGCCCCT GGCCTTCCAG GAAATCCTGG CCTTCCAGGT 
TCAAAGGGTG AACCAGGCTT TCAGGGATTC CCTGGTATGC CAGGTCCCCC AGGTCCCCCT 
GGTTCTCCTG GCCCATCTTT GGAAGGTCCC AAAGGAAACC CTGGGCCTCA AGGCCCTCCT 
GGGAGACCAG GTCCTCCAGG TCCACCTGGT ACAGAAGGTC CTCGTGGCCC CCCTGGAAAT 
GGAGGTATTA AAGGAGAGAG GGGAAATCCA GGTCCACCTG GGCAACCAGG CTTACCTGGC 
TTGAAAGGAG ATCAAGGACC ACCAGGCCTT CCGGGTAACC CTGGCCGACC AGGTCTTAAT 
GGAATGAAAG GAGATCCTGG TCTCCCTGGT GTTCCAGGAT TCCCAGGAAT GAAAGGACCC 
ATTGGAGTAC CAGGCTCAAC TGGCCCTGAT GGGGAACCAG GTCTTACGGG GCCCCCAGGT 
CCTCCTGGGT TGCCTGGTCC TTCAGGACAG AGTATTGTAA TCAAAGGAGA TCCTGGACCT 
CCAGGAATTC CTGGACAACC TGGATTAAAA GGTCTACCAG GACTTCCAGG CCCTCAAGGT 
CTACCAGGTC CAATTGGCCC TCCAGGAGAT CCAGGACGCA ATGGACTCCC AGGCTTTGAT 
GGTGCAGGAG GGCGCAAAGG AGACCCAGGC CTGCCGGGGC AGCCAGGTAG CCGTGGATTG 
GATGGTCCCC CAGGACCAGA TGGATTGCAA GGACCTCCAG GGCCCCCGGG AACCACCTCT 
GTTGCCCATG GATTCCTCAT CACACGTCAC AGTCAGACAA CAGATGCACC ACAATGCCCA 
CAGGGAACAG TCCATATCTA TGATGGCTTT TCTCTCTTAT ATGTACAAGG AAATAAAAGA 
GCCCATGGTC AAGACTTGGG GACAGCTGGC AGCTGCCTTC GTCGCTTCAG TACCATGCCT 
TTCATGTTCT GCAACATTAA CAACGTTTGT AACTTTGCTT CAAGAAATGA CTATTCTTAC 
TGGCTTTCCA CCCCAGAGCC CATGCCAATG AACATGGAAC CCCTGAAGGG CCAGAGCATC 
CAGCCATTCA TTAGTCGATG TGCAGTATGT GAGGCACCAG CTGTGGTGAT TGCAGTTCAC 
AGCCAGACTA TTCAGATTCC TCACTGTCCT CAGGGGTGGG ACTCTCTGTG GATTGGCTAT 
TCATTCATGA TGCATACAAG TGCAGGAGCA GAAGGCTCTG GCCAAGCCCT AGCCTCTCCT 
GGCTCCTGTT TGGAAGAATT TCGTTCAGCT CCCTTCATCG AATGTCATGG ACGAGGGACA 
TGCAATTACT ATGCCAATTC TTACAGCTTT TGGCTGGCCA CTGTTGATAT GTCAGACATG 
TTCAGTAAAC CTCAGTCAGA GACACTGAAA GCAGGCGACT TGAGGACACG TATTAGCCGA 
TGTCAAGTAT GCATGAAAAG GACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006227552.1
CDS	400..5466
Translation	MQVPGVCLAAGWFLLALCLWGQPGEAAACHGCSSGSKCDCSGIKGEKGERGFPGLEGHPGLPGFPGPEGPPGPRGQKGDDGIPGPPGPKGIRGPPGLPGFPGTPGLPGMPGHDGAPGPQGIPGCNGTKGERGFPGSPGFPGLQGPPGPPGIPGMKGEPGSIIMSSLPGPKGNQGYPGPPGIQGPPGPKGVSGPSGPPGPPGLMGPPGPPGPPGPKGNMGLNFQGPKGEKGEQGLQGPPGPPGQISEQKRPIDLDFQKGDQGVPGDRGPPGPPGIPGPPGPPGGVKGEKGEPGEPGKRGKPGKDGETGQPGIPGLPGDPGYPGEPGRHGEKGQKGDTGPPGPPGFVIPTPGTGVTIGAKGNIGQPGLPGEKGERGFPGIQGPPGFPGPPGTAVMGPPGPPGYPGERGQKGDEGPPGICIPGSPGLDGQPGAPGFPGPPGPPGPQLPPSDEICKAGPPGPPGSPGDQGLQGEQGVKGDKGDTCFNCIGTGISGPPGQPGLPGLPGPPGSLGIPGEKGEKGQAGTTGPKGLPGIPGPPGAPGFPGSKGDPGDVLTLPGMKGEKGELGSPGAPGLPGLPGSPGKDGLPGLPGPKGEPGGITFKGERGPPGNPGLPGLPGNMGPTGPVGFGPPGPVGEKGIQGVAGNPGQPGLPGPKGDPGQTISQPGKPGLPGNPGRDGEVGLPGDPGLPGQPGLPGIPGSKGEPGIPGIGPPGPPGPKGFPGIPGPPGPPGAPGRMGPEGPPGLPGFPGPKGEPGFGLPGPPGPPGFPGFKGTPGPKGDRGLPGPPGPPGHTGLDGLPGPKGDAGPNGQPGPIGPPGLPGIGLQGPPGPPGTPGPIGQPGLHGIPGEKGDPGPPGLDVPGLPGERGSPGLPGAPGLIGPPGSPGIPGRAGVPGFPGVKGEMGMMGPPGPPGPLGTPGRSGAPGLKGDDGMQGQPGLPGPVGEKGGKGEPGLPGPPGPMRPDFLGSKGEKGDPGLPGIPGLSGPKGYQGLPGDPGQPGLSGQPGSPGLPGPKGTPGLPGPPGLTGPPGPKGNIGDMGFPGPQGVDGPPGPPGGPGQPGSPGLPGQKGSKGEPGVSGIGLPGLPGPKGEPGLPGYPGNPGIKGSMGEAGLPGLPGSPGAKGQPGLPGFPGTPGLPGPKGMNGPPGNPGLPGEPGPVGGGGRPGPPGPPGEKGKPGQDGIPGPAGQKGEPGQPGFGIPGPPGLPGLSGQKGDGGAPGLPGNPGLPGSKGEPGFQGFPGMPGPPGPPGSPGPSLEGPKGNPGPQGPPGRPGPPGPPGTEGPRGPPGNGGIKGERGNPGPPGQPGLPGLKGDQGPPGLPGNPGRPGLNGMKGDPGLPGVPGFPGMKGPIGVPGSTGPDGEPGLTGPPGPPGLPGPSGQSIVIKGDPGPPGIPGQPGLKGLPGLPGPQGLPGPIGPPGDPGRNGLPGFDGAGGRKGDPGLPGQPGSRGLDGPPGPDGLQGPPGPPGTTSVAHGFLITRHSQTTDAPQCPQGTVHIYDGFSLLYVQGNKRAHGQDLGTAGSCLRRFSTMPFMFCNINNVCNFASRNDYSYWLSTPEPMPMNMEPLKGQSIQPFISRCAVCEAPAVVIAVHSQTIQIPHCPQGWDSLWIGYSFMMHTSAGAEGSGQALASPGSCLEEFRSAPFIECHGRGTCNYYANSYSFWLATVDMSDMFSKPQSETLKAGDLRTRISRCQVCMKRT

Target ORF information:

RefSeq Version	XM_006227490.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus collagen type IV alpha 5 chain (Col4a5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006227490.1

ORF Insert Sequence:

ATGCAAGTGC CTGGAGTGTG CCTGGCCGCC GGCTGGTTCT TACTGGCCCT GTGTCTGTGG 
GGGCAGCCGG GAGAGGCTGC GGCCTGCCAT GGGTGTTCTT CTGGATCAAA ATGTGACTGC 
AGTGGTATAA AAGGAGAAAA GGGAGAACGG GGCTTTCCAG GCTTAGAAGG CCATCCAGGT 
TTACCAGGAT TTCCAGGTCC AGAAGGGCCT CCAGGACCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGA ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGCCTTCC TGGGATGCCA GGCCACGATG GGGCTCCAGG ACCTCAAGGT 
ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGCGGATTTC CAGGCAGTCC AGGCTTTCCC 
GGTTTACAAG GACCTCCAGG ACCACCTGGG ATCCCAGGTA TGAAGGGGGA GCCAGGCAGT 
ATAATCATGT CATCTCTGCC AGGGCCAAAG GGTAATCAAG GATACCCAGG TCCTCCTGGA 
ATACAAGGTC CACCTGGTCC CAAGGGTGTA TCAGGGCCAA GTGGTCCTCC AGGACCACCA 
GGTTTAATGG GCCCTCCTGG CCCACCAGGC CCACCGGGAC CAAAGGGGAA TATGGGCTTA 
AATTTCCAGG GACCCAAAGG CGAAAAAGGT GAACAAGGTC TTCAGGGTCC ACCTGGTCCA 
CCTGGGCAGA TCAGTGAACA GAAAAGACCA ATTGACCTAG ATTTTCAGAA AGGCGATCAG 
GGAGTTCCCG GTGATAGAGG GCCTCCTGGA CCTCCGGGAA TACCTGGTCC TCCCGGTCCT 
CCTGGTGGTG TGAAAGGTGA AAAGGGTGAG CCCGGAGAGC CAGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAGACTGG TCAACCAGGA ATCCCAGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGTGAAC CAGGAAGGCA TGGTGAAAAG GGCCAAAAGG GTGATACTGG CCCACCTGGA 
CCACCTGGAT TTGTAATTCC TACACCTGGA ACTGGTGTGA CTATTGGAGC AAAAGGAAAT 
ATTGGGCAAC CTGGTTTGCC TGGAGAAAAA GGAGAGCGAG GATTTCCTGG CATACAAGGT 
CCACCTGGCT TTCCTGGACC TCCAGGGACA GCTGTCATGG GTCCCCCTGG TCCTCCTGGA 
TATCCTGGTG AAAGGGGCCA GAAAGGTGAT GAAGGTCCCC CTGGAATTTG TATTCCTGGA 
TCTCCTGGAC TTGATGGACA GCCTGGAGCT CCTGGCTTTC CAGGACCTCC TGGTCCTCCT 
GGCCCCCAGT TACCCCCGAG TGATGAAATC TGTAAAGCAG GCCCTCCAGG GCCCCCAGGA 
TCTCCAGGTG ACCAAGGACT CCAAGGAGAG CAAGGAGTAA AAGGTGACAA AGGTGATACT 
TGCTTCAACT GTATTGGAAC TGGCATTTCA GGGCCTCCAG GCCAACCAGG TTTACCAGGT 
CTCCCAGGTC CTCCAGGATC TCTTGGAATC CCTGGAGAGA AGGGCGAGAA AGGACAAGCT 
GGGACAACTG GTCCAAAAGG ATTGCCAGGC ATTCCTGGAC CTCCAGGTGC TCCAGGCTTT 
CCAGGGTCTA AGGGTGACCC TGGTGATGTT CTCACTCTTC CGGGAATGAA AGGTGAAAAA 
GGAGAATTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTC TACCTGGTAG TCCTGGAAAG 
GATGGTTTGC CAGGACTTCC TGGCCCCAAA GGAGAGCCCG GTGGAATCAC TTTTAAGGGT 
GAAAGGGGTC CTCCTGGGAA CCCAGGTTTA CCAGGTCTCC CAGGAAATAT GGGACCTACA 
GGCCCTGTTG GTTTTGGGCC TCCAGGCCCC GTAGGTGAAA AAGGCATACA AGGTGTGGCG 
GGAAATCCAG GCCAGCCAGG ATTACCAGGT CCTAAAGGTG ATCCAGGCCA GACCATCAGC 
CAGCCAGGAA AGCCTGGCTT GCCAGGGAAT CCAGGCAGAG ATGGTGAAGT GGGTCTTCCA 
GGTGATCCTG GACTTCCAGG CCAACCAGGC CTACCAGGAA TACCTGGTAG CAAAGGAGAA 
CCAGGTATCC CTGGGATTGG GCCTCCAGGA CCACCTGGTC CCAAAGGTTT TCCAGGAATT 
CCTGGACCTC CAGGACCTCC TGGGGCCCCT GGAAGAATGG GCCCAGAAGG GCCTCCTGGG 
TTACCGGGCT TTCCAGGACC CAAGGGAGAG CCAGGATTTG GGTTACCTGG GCCACCTGGG 
CCACCAGGAT TCCCAGGTTT CAAAGGAACA CCTGGTCCAA AAGGTGATCG TGGTTTACCA 
GGACCTCCAG GTCCTCCAGG ACACACTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAT 
GCTGGACCAA ATGGACAACC TGGCCCAATA GGGCCACCAG GGCTGCCAGG AATTGGTCTT 
CAGGGACCAC CAGGACCACC AGGGACTCCA GGGCCAATAG GCCAACCTGG CTTACATGGA 
ATACCAGGAG AGAAGGGAGA TCCAGGACCT CCTGGGCTTG ATGTTCCAGG ACTCCCTGGA 
GAGAGAGGTA GTCCAGGGCT CCCTGGAGCC CCTGGTCTTA TAGGACCTCC AGGATCACCT 
GGTATCCCTG GAAGAGCAGG TGTCCCTGGA TTTCCAGGTG TCAAAGGCGA AATGGGTATG 
ATGGGACCTC CAGGCCCACC GGGACCTTTG GGTACTCCTG GCAGGAGTGG TGCTCCTGGT 
CTTAAAGGTG ATGACGGCAT GCAAGGTCAG CCAGGTCTCC CTGGCCCTGT AGGAGAAAAG 
GGTGGTAAGG GAGAACCTGG CCTTCCAGGT CCTCCTGGAC CAATGCGTCC AGATTTCCTG 
GGATCAAAAG GAGAAAAGGG TGATCCTGGC TTACCAGGTA TTCCTGGACT TTCCGGACCA 
AAAGGCTACC AGGGATTACC TGGAGACCCA GGGCAACCTG GGCTGAGTGG ACAGCCGGGA 
TCACCAGGAC TACCAGGTCC CAAAGGTACT CCTGGTCTCC CTGGTCCACC AGGACTTACC 
GGACCTCCTG GACCTAAAGG AAACATTGGC GACATGGGTT TTCCTGGCCC TCAAGGTGTA 
GATGGGCCTC CTGGACCTCC TGGAGGTCCT GGACAACCTG GCTCTCCCGG GTTGCCTGGC 
CAGAAAGGAA GTAAAGGAGA ACCTGGAGTT TCAGGCATTG GTCTCCCTGG TCTTCCTGGT 
CCAAAGGGTG AACCTGGTCT GCCTGGATAT CCAGGAAACC CAGGTATCAA AGGTTCTATG 
GGCGAGGCTG GTCTGCCTGG CTTACCAGGA AGCCCTGGAG CAAAAGGTCA ACCCGGCCTG 
CCCGGATTCC CAGGAACACC AGGGCTTCCT GGACCAAAGG GTATGAATGG TCCTCCTGGT 
AACCCTGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGGCGTCC TGGACCACCA 
GGACCTCCTG GTGAAAAAGG CAAGCCAGGT CAAGATGGCA TTCCTGGACC AGCAGGACAA 
AAGGGAGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CTCCTGGACT CCCAGGACTT 
TCTGGTCAAA AGGGTGATGG AGGAGCCCCT GGCCTTCCAG GAAATCCTGG CCTTCCAGGT 
TCAAAGGGTG AACCAGGCTT TCAGGGATTC CCTGGTATGC CAGGTCCCCC AGGTCCCCCT 
GGTTCTCCTG GCCCATCTTT GGAAGGTCCC AAAGGAAACC CTGGGCCTCA AGGCCCTCCT 
GGGAGACCAG GTCCTCCAGG TCCACCTGGT ACAGAAGGTC CTCGTGGCCC CCCTGGAAAT 
GGAGGTATTA AAGGAGAGAG GGGAAATCCA GGTCCACCTG GGCAACCAGG CTTACCTGGC 
TTGAAAGGAG ATCAAGGACC ACCAGGCCTT CCGGGTAACC CTGGCCGACC AGGTCTTAAT 
GGAATGAAAG GAGATCCTGG TCTCCCTGGT GTTCCAGGAT TCCCAGGAAT GAAAGGACCC 
ATTGGAGTAC CAGGCTCAAC TGGCCCTGAT GGGGAACCAG GTCTTACGGG GCCCCCAGGT 
CCTCCTGGGT TGCCTGGTCC TTCAGGACAG AGTATTGTAA TCAAAGGAGA TCCTGGACCT 
CCAGGAATTC CTGGACAACC TGGATTAAAA GGTCTACCAG GACTTCCAGG CCCTCAAGGT 
CTACCAGGTC CAATTGGCCC TCCAGGAGAT CCAGGACGCA ATGGACTCCC AGGCTTTGAT 
GGTGCAGGAG GGCGCAAAGG AGACCCAGGC CTGCCGGGGC AGCCAGGTAG CCGTGGATTG 
GATGGTCCCC CAGGACCAGA TGGATTGCAA GGACCTCCAG GGCCCCCGGG AACCACCTCT 
GTTGCCCATG GATTCCTCAT CACACGTCAC AGTCAGACAA CAGATGCACC ACAATGCCCA 
CAGGGAACAG TCCATATCTA TGATGGCTTT TCTCTCTTAT ATGTACAAGG AAATAAAAGA 
GCCCATGGTC AAGACTTGGG GACAGCTGGC AGCTGCCTTC GTCGCTTCAG TACCATGCCT 
TTCATGTTCT GCAACATTAA CAACGTTTGT AACTTTGCTT CAAGAAATGA CTATTCTTAC 
TGGCTTTCCA CCCCAGAGCC CATGCCAATG AACATGGAAC CCCTGAAGGG CCAGAGCATC 
CAGCCATTCA TTAGTCGATG TGCAGTATGT GAGGCACCAG CTGTGGTGAT TGCAGTTCAC 
AGCCAGACTA TTCAGATTCC TCACTGTCCT CAGGGGTGGG ACTCTCTGTG GATTGGCTAT 
TCATTCATGA TGCATACAAG TGCAGGAGCA GAAGGCTCTG GCCAAGCCCT AGCCTCTCCT 
GGCTCCTGTT TGGAAGAATT TCGTTCAGCT CCCTTCATCG AATGTCATGG ACGAGGGACA 
TGCAATTACT ATGCCAATTC TTACAGCTTT TGGCTGGCCA CTGTTGATAT GTCAGACATG 
TTCAGTAAAC CTCAGTCAGA GACACTGAAA GCAGGCGACT TGAGGACACG TATTAGCCGA 
TGTCAAGTAT GCATGAAAAG GACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col4a5 ( XM_006257312.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006257374.1 Rattus norvegicus collagen type IV alpha 5 chain (Col4a5), transcript variant X2, mRNA.

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CloneID	ORa21618
Clone ID Related Accession (Same CDS sequence)	XM_006227490.1 , XM_006257312.1
Accession Version	XM_006257312.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5067bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	collagen alpha-5(IV) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGTGC CTGGAGTGTG CCTGGCCGCC GGCTGGTTCT TACTGGCCCT GTGTCTGTGG 
GGGCAGCCGG GAGAGGCTGC GGCCTGCCAT GGGTGTTCTT CTGGATCAAA ATGTGACTGC 
AGTGGTATAA AAGGAGAAAA GGGAGAACGG GGCTTTCCAG GCTTAGAAGG CCATCCAGGT 
TTACCAGGAT TTCCAGGTCC AGAAGGGCCT CCAGGACCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGA ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGCCTTCC TGGGATGCCA GGCCACGATG GGGCTCCAGG ACCTCAAGGT 
ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGCGGATTTC CAGGCAGTCC AGGCTTTCCC 
GGTTTACAAG GACCTCCAGG ACCACCTGGG ATCCCAGGTA TGAAGGGGGA GCCAGGCAGT 
ATAATCATGT CATCTCTGCC AGGGCCAAAG GGTAATCAAG GATACCCAGG TCCTCCTGGA 
ATACAAGGTC CACCTGGTCC CAAGGGTGTA TCAGGGCCAA GTGGTCCTCC AGGACCACCA 
GGTTTAATGG GCCCTCCTGG CCCACCAGGC CCACCGGGAC CAAAGGGGAA TATGGGCTTA 
AATTTCCAGG GACCCAAAGG CGAAAAAGGT GAACAAGGTC TTCAGGGTCC ACCTGGTCCA 
CCTGGGCAGA TCAGTGAACA GAAAAGACCA ATTGACCTAG ATTTTCAGAA AGGCGATCAG 
GGAGTTCCCG GTGATAGAGG GCCTCCTGGA CCTCCGGGAA TACCTGGTCC TCCCGGTCCT 
CCTGGTGGTG TGAAAGGTGA AAAGGGTGAG CCCGGAGAGC CAGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAGACTGG TCAACCAGGA ATCCCAGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGTGAAC CAGGAAGGCA TGGTGAAAAG GGCCAAAAGG GTGATACTGG CCCACCTGGA 
CCACCTGGAT TTGTAATTCC TACACCTGGA ACTGGTGTGA CTATTGGAGC AAAAGGAAAT 
ATTGGGCAAC CTGGTTTGCC TGGAGAAAAA GGAGAGCGAG GATTTCCTGG CATACAAGGT 
CCACCTGGCT TTCCTGGACC TCCAGGGACA GCTGTCATGG GTCCCCCTGG TCCTCCTGGA 
TATCCTGGTG AAAGGGGCCA GAAAGGTGAT GAAGGTCCCC CTGGAATTTG TATTCCTGGA 
TCTCCTGGAC TTGATGGACA GCCTGGAGCT CCTGGCTTTC CAGGACCTCC TGGTCCTCCT 
GGCCCCCAGT TACCCCCGAG TGATGAAATC TGTAAAGCAG GCCCTCCAGG GCCCCCAGGA 
TCTCCAGGTG ACCAAGGACT CCAAGGAGAG CAAGGAGTAA AAGGTGACAA AGGTGATACT 
TGCTTCAACT GTATTGGAAC TGGCATTTCA GGGCCTCCAG GCCAACCAGG TTTACCAGGT 
CTCCCAGGTC CTCCAGGATC TCTTGGAATC CCTGGAGAGA AGGGCGAGAA AGGACAAGCT 
GGGACAACTG GTCCAAAAGG ATTGCCAGGC ATTCCTGGAC CTCCAGGTGC TCCAGGCTTT 
CCAGGGTCTA AGGGTGACCC TGGTGATGTT CTCACTCTTC CGGGAATGAA AGGTGAAAAA 
GGAGAATTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTC TACCTGGTAG TCCTGGAAAG 
GATGGTTTGC CAGGACTTCC TGGCCCCAAA GGAGAGCCCG GTGGAATCAC TTTTAAGGGT 
GAAAGGGGTC CTCCTGGGAA CCCAGGTTTA CCAGGTCTCC CAGGAAATAT GGGACCTACA 
GGCCCTGTTG GTTTTGGGCC TCCAGGCCCC GTAGGTGAAA AAGGCATACA AGGTGTGGCG 
GGAAATCCAG GCCAGCCAGG ATTACCAGGT CCTAAAGGTG ATCCAGGCCA GACCATCAGC 
CAGCCAGGAA AGCCTGGCTT GCCAGGGAAT CCAGGCAGAG ATGGTGAAGT GGGTCTTCCA 
GGTGATCCTG GACTTCCAGG CCAACCAGGC CTACCAGGAA TACCTGGTAG CAAAGGAGAA 
CCAGGTATCC CTGGGATTGG GCCTCCAGGA CCACCTGGTC CCAAAGGTTT TCCAGGAATT 
CCTGGACCTC CAGGACCTCC TGGGGCCCCT GGAAGAATGG GCCCAGAAGG GCCTCCTGGG 
TTACCGGGCT TTCCAGGACC CAAGGGAGAG CCAGGATTTG GGTTACCTGG GCCACCTGGG 
CCACCAGGAT TCCCAGGTTT CAAAGGAACA CCTGGTCCAA AAGGTGATCG TGGTTTACCA 
GGACCTCCAG GTCCTCCAGG ACACACTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAT 
GCTGGACCAA ATGGACAACC TGGCCCAATA GGGCCACCAG GGCTGCCAGG AATTGGTCTT 
CAGGGACCAC CAGGACCACC AGGGACTCCA GGGCCAATAG GCCAACCTGG CTTACATGGA 
ATACCAGGAG AGAAGGGAGA TCCAGGACCT CCTGGGCTTG ATGTTCCAGG ACTCCCTGGA 
GAGAGAGGTA GTCCAGGGCT CCCTGGAGCC CCTGGTCTTA TAGGACCTCC AGGATCACCT 
GGTATCCCTG GAAGAGCAGG TGTCCCTGGA TTTCCAGGTG TCAAAGGCGA AATGGGTATG 
ATGGGACCTC CAGGCCCACC GGGACCTTTG GGTACTCCTG GCAGGAGTGG TGCTCCTGGT 
CTTAAAGGTG ATGACGGCAT GCAAGGTCAG CCAGGTCTCC CTGGCCCTGT AGGAGAAAAG 
GGTGGTAAGG GAGAACCTGG CCTTCCAGGT CCTCCTGGAC CAATGCGTCC AGATTTCCTG 
GGATCAAAAG GAGAAAAGGG TGATCCTGGC TTACCAGGTA TTCCTGGACT TTCCGGACCA 
AAAGGCTACC AGGGATTACC TGGAGACCCA GGGCAACCTG GGCTGAGTGG ACAGCCGGGA 
TCACCAGGAC TACCAGGTCC CAAAGGTACT CCTGGTCTCC CTGGTCCACC AGGACTTACC 
GGACCTCCTG GACCTAAAGG AAACATTGGC GACATGGGTT TTCCTGGCCC TCAAGGTGTA 
GATGGGCCTC CTGGACCTCC TGGAGGTCCT GGACAACCTG GCTCTCCCGG GTTGCCTGGC 
CAGAAAGGAA GTAAAGGAGA ACCTGGAGTT TCAGGCATTG GTCTCCCTGG TCTTCCTGGT 
CCAAAGGGTG AACCTGGTCT GCCTGGATAT CCAGGAAACC CAGGTATCAA AGGTTCTATG 
GGCGAGGCTG GTCTGCCTGG CTTACCAGGA AGCCCTGGAG CAAAAGGTCA ACCCGGCCTG 
CCCGGATTCC CAGGAACACC AGGGCTTCCT GGACCAAAGG GTATGAATGG TCCTCCTGGT 
AACCCTGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGGCGTCC TGGACCACCA 
GGACCTCCTG GTGAAAAAGG CAAGCCAGGT CAAGATGGCA TTCCTGGACC AGCAGGACAA 
AAGGGAGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CTCCTGGACT CCCAGGACTT 
TCTGGTCAAA AGGGTGATGG AGGAGCCCCT GGCCTTCCAG GAAATCCTGG CCTTCCAGGT 
TCAAAGGGTG AACCAGGCTT TCAGGGATTC CCTGGTATGC CAGGTCCCCC AGGTCCCCCT 
GGTTCTCCTG GCCCATCTTT GGAAGGTCCC AAAGGAAACC CTGGGCCTCA AGGCCCTCCT 
GGGAGACCAG GTCCTCCAGG TCCACCTGGT ACAGAAGGTC CTCGTGGCCC CCCTGGAAAT 
GGAGGTATTA AAGGAGAGAG GGGAAATCCA GGTCCACCTG GGCAACCAGG CTTACCTGGC 
TTGAAAGGAG ATCAAGGACC ACCAGGCCTT CCGGGTAACC CTGGCCGACC AGGTCTTAAT 
GGAATGAAAG GAGATCCTGG TCTCCCTGGT GTTCCAGGAT TCCCAGGAAT GAAAGGACCC 
ATTGGAGTAC CAGGCTCAAC TGGCCCTGAT GGGGAACCAG GTCTTACGGG GCCCCCAGGT 
CCTCCTGGGT TGCCTGGTCC TTCAGGACAG AGTATTGTAA TCAAAGGAGA TCCTGGACCT 
CCAGGAATTC CTGGACAACC TGGATTAAAA GGTCTACCAG GACTTCCAGG CCCTCAAGGT 
CTACCAGGTC CAATTGGCCC TCCAGGAGAT CCAGGACGCA ATGGACTCCC AGGCTTTGAT 
GGTGCAGGAG GGCGCAAAGG AGACCCAGGC CTGCCGGGGC AGCCAGGTAG CCGTGGATTG 
GATGGTCCCC CAGGACCAGA TGGATTGCAA GGACCTCCAG GGCCCCCGGG AACCACCTCT 
GTTGCCCATG GATTCCTCAT CACACGTCAC AGTCAGACAA CAGATGCACC ACAATGCCCA 
CAGGGAACAG TCCATATCTA TGATGGCTTT TCTCTCTTAT ATGTACAAGG AAATAAAAGA 
GCCCATGGTC AAGACTTGGG GACAGCTGGC AGCTGCCTTC GTCGCTTCAG TACCATGCCT 
TTCATGTTCT GCAACATTAA CAACGTTTGT AACTTTGCTT CAAGAAATGA CTATTCTTAC 
TGGCTTTCCA CCCCAGAGCC CATGCCAATG AACATGGAAC CCCTGAAGGG CCAGAGCATC 
CAGCCATTCA TTAGTCGATG TGCAGTATGT GAGGCACCAG CTGTGGTGAT TGCAGTTCAC 
AGCCAGACTA TTCAGATTCC TCACTGTCCT CAGGGGTGGG ACTCTCTGTG GATTGGCTAT 
TCATTCATGA TGCATACAAG TGCAGGAGCA GAAGGCTCTG GCCAAGCCCT AGCCTCTCCT 
GGCTCCTGTT TGGAAGAATT TCGTTCAGCT CCCTTCATCG AATGTCATGG ACGAGGGACA 
TGCAATTACT ATGCCAATTC TTACAGCTTT TGGCTGGCCA CTGTTGATAT GTCAGACATG 
TTCAGTAAAC CTCAGTCAGA GACACTGAAA GCAGGCGACT TGAGGACACG TATTAGCCGA 
TGTCAAGTAT GCATGAAAAG GACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006257374.1
CDS	400..5466
Translation	MQVPGVCLAAGWFLLALCLWGQPGEAAACHGCSSGSKCDCSGIKGEKGERGFPGLEGHPGLPGFPGPEGPPGPRGQKGDDGIPGPPGPKGIRGPPGLPGFPGTPGLPGMPGHDGAPGPQGIPGCNGTKGERGFPGSPGFPGLQGPPGPPGIPGMKGEPGSIIMSSLPGPKGNQGYPGPPGIQGPPGPKGVSGPSGPPGPPGLMGPPGPPGPPGPKGNMGLNFQGPKGEKGEQGLQGPPGPPGQISEQKRPIDLDFQKGDQGVPGDRGPPGPPGIPGPPGPPGGVKGEKGEPGEPGKRGKPGKDGETGQPGIPGLPGDPGYPGEPGRHGEKGQKGDTGPPGPPGFVIPTPGTGVTIGAKGNIGQPGLPGEKGERGFPGIQGPPGFPGPPGTAVMGPPGPPGYPGERGQKGDEGPPGICIPGSPGLDGQPGAPGFPGPPGPPGPQLPPSDEICKAGPPGPPGSPGDQGLQGEQGVKGDKGDTCFNCIGTGISGPPGQPGLPGLPGPPGSLGIPGEKGEKGQAGTTGPKGLPGIPGPPGAPGFPGSKGDPGDVLTLPGMKGEKGELGSPGAPGLPGLPGSPGKDGLPGLPGPKGEPGGITFKGERGPPGNPGLPGLPGNMGPTGPVGFGPPGPVGEKGIQGVAGNPGQPGLPGPKGDPGQTISQPGKPGLPGNPGRDGEVGLPGDPGLPGQPGLPGIPGSKGEPGIPGIGPPGPPGPKGFPGIPGPPGPPGAPGRMGPEGPPGLPGFPGPKGEPGFGLPGPPGPPGFPGFKGTPGPKGDRGLPGPPGPPGHTGLDGLPGPKGDAGPNGQPGPIGPPGLPGIGLQGPPGPPGTPGPIGQPGLHGIPGEKGDPGPPGLDVPGLPGERGSPGLPGAPGLIGPPGSPGIPGRAGVPGFPGVKGEMGMMGPPGPPGPLGTPGRSGAPGLKGDDGMQGQPGLPGPVGEKGGKGEPGLPGPPGPMRPDFLGSKGEKGDPGLPGIPGLSGPKGYQGLPGDPGQPGLSGQPGSPGLPGPKGTPGLPGPPGLTGPPGPKGNIGDMGFPGPQGVDGPPGPPGGPGQPGSPGLPGQKGSKGEPGVSGIGLPGLPGPKGEPGLPGYPGNPGIKGSMGEAGLPGLPGSPGAKGQPGLPGFPGTPGLPGPKGMNGPPGNPGLPGEPGPVGGGGRPGPPGPPGEKGKPGQDGIPGPAGQKGEPGQPGFGIPGPPGLPGLSGQKGDGGAPGLPGNPGLPGSKGEPGFQGFPGMPGPPGPPGSPGPSLEGPKGNPGPQGPPGRPGPPGPPGTEGPRGPPGNGGIKGERGNPGPPGQPGLPGLKGDQGPPGLPGNPGRPGLNGMKGDPGLPGVPGFPGMKGPIGVPGSTGPDGEPGLTGPPGPPGLPGPSGQSIVIKGDPGPPGIPGQPGLKGLPGLPGPQGLPGPIGPPGDPGRNGLPGFDGAGGRKGDPGLPGQPGSRGLDGPPGPDGLQGPPGPPGTTSVAHGFLITRHSQTTDAPQCPQGTVHIYDGFSLLYVQGNKRAHGQDLGTAGSCLRRFSTMPFMFCNINNVCNFASRNDYSYWLSTPEPMPMNMEPLKGQSIQPFISRCAVCEAPAVVIAVHSQTIQIPHCPQGWDSLWIGYSFMMHTSAGAEGSGQALASPGSCLEEFRSAPFIECHGRGTCNYYANSYSFWLATVDMSDMFSKPQSETLKAGDLRTRISRCQVCMKRT

Target ORF information:

RefSeq Version	XM_006257312.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus collagen type IV alpha 5 chain (Col4a5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006257312.1

ORF Insert Sequence:

ATGCAAGTGC CTGGAGTGTG CCTGGCCGCC GGCTGGTTCT TACTGGCCCT GTGTCTGTGG 
GGGCAGCCGG GAGAGGCTGC GGCCTGCCAT GGGTGTTCTT CTGGATCAAA ATGTGACTGC 
AGTGGTATAA AAGGAGAAAA GGGAGAACGG GGCTTTCCAG GCTTAGAAGG CCATCCAGGT 
TTACCAGGAT TTCCAGGTCC AGAAGGGCCT CCAGGACCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGA ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGCCTTCC TGGGATGCCA GGCCACGATG GGGCTCCAGG ACCTCAAGGT 
ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGCGGATTTC CAGGCAGTCC AGGCTTTCCC 
GGTTTACAAG GACCTCCAGG ACCACCTGGG ATCCCAGGTA TGAAGGGGGA GCCAGGCAGT 
ATAATCATGT CATCTCTGCC AGGGCCAAAG GGTAATCAAG GATACCCAGG TCCTCCTGGA 
ATACAAGGTC CACCTGGTCC CAAGGGTGTA TCAGGGCCAA GTGGTCCTCC AGGACCACCA 
GGTTTAATGG GCCCTCCTGG CCCACCAGGC CCACCGGGAC CAAAGGGGAA TATGGGCTTA 
AATTTCCAGG GACCCAAAGG CGAAAAAGGT GAACAAGGTC TTCAGGGTCC ACCTGGTCCA 
CCTGGGCAGA TCAGTGAACA GAAAAGACCA ATTGACCTAG ATTTTCAGAA AGGCGATCAG 
GGAGTTCCCG GTGATAGAGG GCCTCCTGGA CCTCCGGGAA TACCTGGTCC TCCCGGTCCT 
CCTGGTGGTG TGAAAGGTGA AAAGGGTGAG CCCGGAGAGC CAGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAGACTGG TCAACCAGGA ATCCCAGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGTGAAC CAGGAAGGCA TGGTGAAAAG GGCCAAAAGG GTGATACTGG CCCACCTGGA 
CCACCTGGAT TTGTAATTCC TACACCTGGA ACTGGTGTGA CTATTGGAGC AAAAGGAAAT 
ATTGGGCAAC CTGGTTTGCC TGGAGAAAAA GGAGAGCGAG GATTTCCTGG CATACAAGGT 
CCACCTGGCT TTCCTGGACC TCCAGGGACA GCTGTCATGG GTCCCCCTGG TCCTCCTGGA 
TATCCTGGTG AAAGGGGCCA GAAAGGTGAT GAAGGTCCCC CTGGAATTTG TATTCCTGGA 
TCTCCTGGAC TTGATGGACA GCCTGGAGCT CCTGGCTTTC CAGGACCTCC TGGTCCTCCT 
GGCCCCCAGT TACCCCCGAG TGATGAAATC TGTAAAGCAG GCCCTCCAGG GCCCCCAGGA 
TCTCCAGGTG ACCAAGGACT CCAAGGAGAG CAAGGAGTAA AAGGTGACAA AGGTGATACT 
TGCTTCAACT GTATTGGAAC TGGCATTTCA GGGCCTCCAG GCCAACCAGG TTTACCAGGT 
CTCCCAGGTC CTCCAGGATC TCTTGGAATC CCTGGAGAGA AGGGCGAGAA AGGACAAGCT 
GGGACAACTG GTCCAAAAGG ATTGCCAGGC ATTCCTGGAC CTCCAGGTGC TCCAGGCTTT 
CCAGGGTCTA AGGGTGACCC TGGTGATGTT CTCACTCTTC CGGGAATGAA AGGTGAAAAA 
GGAGAATTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTC TACCTGGTAG TCCTGGAAAG 
GATGGTTTGC CAGGACTTCC TGGCCCCAAA GGAGAGCCCG GTGGAATCAC TTTTAAGGGT 
GAAAGGGGTC CTCCTGGGAA CCCAGGTTTA CCAGGTCTCC CAGGAAATAT GGGACCTACA 
GGCCCTGTTG GTTTTGGGCC TCCAGGCCCC GTAGGTGAAA AAGGCATACA AGGTGTGGCG 
GGAAATCCAG GCCAGCCAGG ATTACCAGGT CCTAAAGGTG ATCCAGGCCA GACCATCAGC 
CAGCCAGGAA AGCCTGGCTT GCCAGGGAAT CCAGGCAGAG ATGGTGAAGT GGGTCTTCCA 
GGTGATCCTG GACTTCCAGG CCAACCAGGC CTACCAGGAA TACCTGGTAG CAAAGGAGAA 
CCAGGTATCC CTGGGATTGG GCCTCCAGGA CCACCTGGTC CCAAAGGTTT TCCAGGAATT 
CCTGGACCTC CAGGACCTCC TGGGGCCCCT GGAAGAATGG GCCCAGAAGG GCCTCCTGGG 
TTACCGGGCT TTCCAGGACC CAAGGGAGAG CCAGGATTTG GGTTACCTGG GCCACCTGGG 
CCACCAGGAT TCCCAGGTTT CAAAGGAACA CCTGGTCCAA AAGGTGATCG TGGTTTACCA 
GGACCTCCAG GTCCTCCAGG ACACACTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAT 
GCTGGACCAA ATGGACAACC TGGCCCAATA GGGCCACCAG GGCTGCCAGG AATTGGTCTT 
CAGGGACCAC CAGGACCACC AGGGACTCCA GGGCCAATAG GCCAACCTGG CTTACATGGA 
ATACCAGGAG AGAAGGGAGA TCCAGGACCT CCTGGGCTTG ATGTTCCAGG ACTCCCTGGA 
GAGAGAGGTA GTCCAGGGCT CCCTGGAGCC CCTGGTCTTA TAGGACCTCC AGGATCACCT 
GGTATCCCTG GAAGAGCAGG TGTCCCTGGA TTTCCAGGTG TCAAAGGCGA AATGGGTATG 
ATGGGACCTC CAGGCCCACC GGGACCTTTG GGTACTCCTG GCAGGAGTGG TGCTCCTGGT 
CTTAAAGGTG ATGACGGCAT GCAAGGTCAG CCAGGTCTCC CTGGCCCTGT AGGAGAAAAG 
GGTGGTAAGG GAGAACCTGG CCTTCCAGGT CCTCCTGGAC CAATGCGTCC AGATTTCCTG 
GGATCAAAAG GAGAAAAGGG TGATCCTGGC TTACCAGGTA TTCCTGGACT TTCCGGACCA 
AAAGGCTACC AGGGATTACC TGGAGACCCA GGGCAACCTG GGCTGAGTGG ACAGCCGGGA 
TCACCAGGAC TACCAGGTCC CAAAGGTACT CCTGGTCTCC CTGGTCCACC AGGACTTACC 
GGACCTCCTG GACCTAAAGG AAACATTGGC GACATGGGTT TTCCTGGCCC TCAAGGTGTA 
GATGGGCCTC CTGGACCTCC TGGAGGTCCT GGACAACCTG GCTCTCCCGG GTTGCCTGGC 
CAGAAAGGAA GTAAAGGAGA ACCTGGAGTT TCAGGCATTG GTCTCCCTGG TCTTCCTGGT 
CCAAAGGGTG AACCTGGTCT GCCTGGATAT CCAGGAAACC CAGGTATCAA AGGTTCTATG 
GGCGAGGCTG GTCTGCCTGG CTTACCAGGA AGCCCTGGAG CAAAAGGTCA ACCCGGCCTG 
CCCGGATTCC CAGGAACACC AGGGCTTCCT GGACCAAAGG GTATGAATGG TCCTCCTGGT 
AACCCTGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGGCGTCC TGGACCACCA 
GGACCTCCTG GTGAAAAAGG CAAGCCAGGT CAAGATGGCA TTCCTGGACC AGCAGGACAA 
AAGGGAGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CTCCTGGACT CCCAGGACTT 
TCTGGTCAAA AGGGTGATGG AGGAGCCCCT GGCCTTCCAG GAAATCCTGG CCTTCCAGGT 
TCAAAGGGTG AACCAGGCTT TCAGGGATTC CCTGGTATGC CAGGTCCCCC AGGTCCCCCT 
GGTTCTCCTG GCCCATCTTT GGAAGGTCCC AAAGGAAACC CTGGGCCTCA AGGCCCTCCT 
GGGAGACCAG GTCCTCCAGG TCCACCTGGT ACAGAAGGTC CTCGTGGCCC CCCTGGAAAT 
GGAGGTATTA AAGGAGAGAG GGGAAATCCA GGTCCACCTG GGCAACCAGG CTTACCTGGC 
TTGAAAGGAG ATCAAGGACC ACCAGGCCTT CCGGGTAACC CTGGCCGACC AGGTCTTAAT 
GGAATGAAAG GAGATCCTGG TCTCCCTGGT GTTCCAGGAT TCCCAGGAAT GAAAGGACCC 
ATTGGAGTAC CAGGCTCAAC TGGCCCTGAT GGGGAACCAG GTCTTACGGG GCCCCCAGGT 
CCTCCTGGGT TGCCTGGTCC TTCAGGACAG AGTATTGTAA TCAAAGGAGA TCCTGGACCT 
CCAGGAATTC CTGGACAACC TGGATTAAAA GGTCTACCAG GACTTCCAGG CCCTCAAGGT 
CTACCAGGTC CAATTGGCCC TCCAGGAGAT CCAGGACGCA ATGGACTCCC AGGCTTTGAT 
GGTGCAGGAG GGCGCAAAGG AGACCCAGGC CTGCCGGGGC AGCCAGGTAG CCGTGGATTG 
GATGGTCCCC CAGGACCAGA TGGATTGCAA GGACCTCCAG GGCCCCCGGG AACCACCTCT 
GTTGCCCATG GATTCCTCAT CACACGTCAC AGTCAGACAA CAGATGCACC ACAATGCCCA 
CAGGGAACAG TCCATATCTA TGATGGCTTT TCTCTCTTAT ATGTACAAGG AAATAAAAGA 
GCCCATGGTC AAGACTTGGG GACAGCTGGC AGCTGCCTTC GTCGCTTCAG TACCATGCCT 
TTCATGTTCT GCAACATTAA CAACGTTTGT AACTTTGCTT CAAGAAATGA CTATTCTTAC 
TGGCTTTCCA CCCCAGAGCC CATGCCAATG AACATGGAAC CCCTGAAGGG CCAGAGCATC 
CAGCCATTCA TTAGTCGATG TGCAGTATGT GAGGCACCAG CTGTGGTGAT TGCAGTTCAC 
AGCCAGACTA TTCAGATTCC TCACTGTCCT CAGGGGTGGG ACTCTCTGTG GATTGGCTAT 
TCATTCATGA TGCATACAAG TGCAGGAGCA GAAGGCTCTG GCCAAGCCCT AGCCTCTCCT 
GGCTCCTGTT TGGAAGAATT TCGTTCAGCT CCCTTCATCG AATGTCATGG ACGAGGGACA 
TGCAATTACT ATGCCAATTC TTACAGCTTT TGGCTGGCCA CTGTTGATAT GTCAGACATG 
TTCAGTAAAC CTCAGTCAGA GACACTGAAA GCAGGCGACT TGAGGACACG TATTAGCCGA 
TGTCAAGTAT GCATGAAAAG GACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col4a5 ( XM_006257313.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006257375.1 Rattus norvegicus collagen type IV alpha 5 chain (Col4a5), transcript variant X4, mRNA.

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CloneID	ORa21619
Clone ID Related Accession (Same CDS sequence)	XM_006257313.1 , XM_006227491.1
Accession Version	XM_006257313.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	collagen alpha-5(IV) chain isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGTGC CTGGAGTGTG CCTGGCCGCC GGCTGGTTCT TACTGGCCCT GTGTCTGTGG 
GGGCAGCCGG GAGAGGCTGC GGCCTGCCAT GGGTGTTCTT CTGGATCAAA ATGTGACTGC 
AGTGGTATAA AAGGAGAAAA GGGAGAACGG GGCTTTCCAG GCTTAGAAGG CCATCCAGGT 
TTACCAGGAT TTCCAGGTCC AGAAGGGCCT CCAGGACCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGA ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGCCTTCC TGGGATGCCA GGCCACGATG GGGCTCCAGG ACCTCAAGGT 
ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGCGGATTTC CAGGCAGTCC AGGCTTTCCC 
GGTTTACAAG GACCTCCAGG ACCACCTGGG ATCCCAGGTA TGAAGGGGGA GCCAGGCAGT 
ATAATCATGT CATCTCTGCC AGGGCCAAAG GGTAATCAAG GATACCCAGG TCCTCCTGGA 
ATACAAGGTC CACCTGGTCC CAAGGGTGTA TCAGGGCCAA GTGGTCCTCC AGGACCACCA 
GGTTTAATGG GCCCTCCTGG CCCACCAGGC CCACCGGGAC CAAAGGGGAA TATGGGCTTA 
AATTTCCAGG GACCCAAAGG CGAAAAAGGT GAACAAGGTC TTCAGGGTCC ACCTGGTCCA 
CCTGGGCAGA TCAGTGAACA GAAAAGACCA ATTGACCTAG ATTTTCAGAA AGGCGATCAG 
GGAGTTCCCG GTGATAGAGG GCCTCCTGGA CCTCCGGGAA TACCTGGTCC TCCCGGTCCT 
CCTGGTGGTG TGAAAGGTGA AAAGGGTGAG CCCGGAGAGC CAGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAGACTGG TCAACCAGGA ATCCCAGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGTGAAC CAGGAAGGCA TGGTGAAAAG GGCCAAAAGG GTGATACTGG CCCACCTGGA 
CCACCTGGAT TTGTAATTCC TACACCTGGA ACTGGTGTGA CTATTGGAGC AAAAGGAAAT 
ATTGGGCAAC CTGGTTTGCC TGGAGAAAAA GGAGAGCGAG GATTTCCTGG CATACAAGGT 
CCACCTGGCT TTCCTGGACC TCCAGGGACA GCTGTCATGG GTCCCCCTGG TCCTCCTGGA 
TATCCTGGTG AAAGGGGCCA GAAAGGTGAT GAAGGTCCCC CTGGAATTTG TATTCCTGGA 
TCTCCTGGAC TTGATGGACA GCCTGGAGCT CCTGGCTTTC CAGGACCTCC TGGTCCTCCT 
GGCCCCCAGT TACCCCCGAG TGATGAAATC TGTAAAGCAG GCCCTCCAGG GCCCCCAGGA 
TCTCCAGGTG ACCAAGGACT CCAAGGAGAG CAAGGAGTAA AAGGTGACAA AGGTGATACT 
TGCTTCAACT GTATTGGAAC TGGCATTTCA GGGCCTCCAG GCCAACCAGG TTTACCAGGT 
CTCCCAGGTC CTCCAGGATC TCTTGGAATC CCTGGAGAGA AGGGCGAGAA AGGACAAGCT 
GGGACAACTG GTCCAAAAGG ATTGCCAGGC ATTCCTGGAC CTCCAGGTGC TCCAGGCTTT 
CCAGGGTCTA AGGGTGACCC TGGTGATGTT CTCACTCTTC CGGGAATGAA AGGTGAAAAA 
GGAGAATTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTC TACCTGGTAG TCCTGGAAAG 
GATGGTTTGC CAGGACTTCC TGGCCCCAAA GGAGAGCCCG GTGGAATCAC TTTTAAGGGT 
GAAAGGGGTC CTCCTGGGAA CCCAGGTTTA CCAGGTCTCC CAGGAAATAT GGGACCTACA 
GGCCCTGTTG GTTTTGGGCC TCCAGGCCCC GTAGGTGAAA AAGGCATACA AGGTGTGGCG 
GGAAATCCAG GCCAGCCAGG ATTACCAGGT CCTAAAGGTG ATCCAGGCCA GACCATCAGC 
CAGCCAGGAA AGCCTGGCTT GCCAGGGAAT CCAGGCAGAG ATGGTGAAGT GGGTCTTCCA 
GGTGATCCTG GACTTCCAGG CCAACCAGGC CTACCAGGAA TACCTGGTAG CAAAGGAGAA 
CCAGGTATCC CTGGGATTGG GCCTCCAGGA CCACCTGGTC CCAAAGGTTT TCCAGGAATT 
CCTGGACCTC CAGGACCTCC TGGGGCCCCT GGAAGAATGG GCCCAGAAGG GCCTCCTGGG 
TTACCGGGCT TTCCAGGACC CAAGGGAGAG CCAGGATTTG GGTTACCTGG GCCACCTGGG 
CCACCAGGAT TCCCAGGTTT CAAAGGAACA CCTGGTCCAA AAGGTGATCG TGGTTTACCA 
GGACCTCCAG GTCCTCCAGG ACACACTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAT 
GCTGGACCAA ATGGACAACC TGGCCCAATA GGGCCACCAG GGCTGCCAGG AATTGGTCTT 
CAGGGACCAC CAGGACCACC AGGGACTCCA GGGCCAATAG GCCAACCTGG CTTACATGGA 
ATACCAGGAG AGAAGGGAGA TCCAGGACCT CCTGGGCTTG ATGTTCCAGG ACTCCCTGGA 
GAGAGAGGTA GTCCAGGGCT CCCTGGAGCC CCTGGTCTTA TAGGACCTCC AGGATCACCT 
GGTATCCCTG GAAGAGCAGG TGTCCCTGGA TTTCCAGGTG TCAAAGGCGA AATGGGTATG 
ATGGGACCTC CAGGCCCACC GGGACCTTTG GGTACTCCTG GCAGGAGTGG TGCTCCTGGT 
CTTAAAGGTG ATGACGGCAT GCAAGGTCAG CCAGGTCTCC CTGGCCCTGT AGGAGAAAAG 
GGTGGTAAGG GAGAACCTGG CCTTCCAGGT CCTCCTGGAC CAATGCGTCC AGATTTCCTG 
GGATCAAAAG GAGAAAAGGG TGATCCTGGC TTACCAGGTA TTCCTGGACT TTCCGGACCA 
AAAGGCTACC AGGGATTACC TGGAGACCCA GGGCAACCTG GGCTGAGTGG ACAGCCGGGA 
TCACCAGGAC TACCAGGTCC CAAAGGTACT CCTGGTCTCC CTGGTCCACC AGGACTTACC 
GGACCTCCTG GACCTAAAGG AAACATTGGC GACATGGGTT TTCCTGGCCC TCAAGGTGTA 
GATGGGCCTC CTGGACCTCC TGGAGGTCCT GGACAACCTG GCTCTCCCGG GTTGCCTGGC 
CAGAAAGGAA GTAAAGGAGA ACCTGGAGTT TCAGGCATTG GTCTCCCTGG TCTTCCTGGT 
CCAAAGGGTG AACCTGGTCT GCCTGGATAT CCAGGAAACC CAGGTATCAA AGGTTCTATG 
GGCGAGGCTG GTCTGCCTGG CTTACCAGGA AGCCCTGGAG CAAAAGGTCA ACCCGGCCTG 
CCCGGATTCC CAGGAACACC AGGGCTTCCT GGACCAAAGG GTATGAATGG TCCTCCTGGT 
AACCCTGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGGCGTCC TGGACCACCA 
GGACCTCCTG GTGAAAAAGG CAAGCCAGGT CAAGATGGCA TTCCTGGACC AGCAGGACAA 
AAGGGAGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CTCCTGGACT CCCAGGACTT 
TCTGGTCAAA AGGGTGATGG AGGAGCCCCT GGCCTTCCAG GAAATCCTGG CCTTCCAGGT 
TCAAAGGGTG AACCAGGCTT TCAGGGATTC CCTGGTATGC CAGGTCCCCC AGGTCCCCCT 
GGTTCTCCTG GCCCATCTTT GGAAGGTCCC AAAGGAAACC CTGGGCCTCA AGGCCCTCCT 
GGGAGACCAG GTCCACCTGG TACAGAAGGT CCTCGTGGCC CCCCTGGAAA TGGAGGTATT 
AAAGGAGAGA GGGGAAATCC AGGTCCACCT GGGCAACCAG GCTTACCTGG CTTGAAAGGA 
GATCAAGGAC CACCAGGCCT TCCGGGTAAC CCTGGCCGAC CAGGTCTTAA TGGAATGAAA 
GGAGATCCTG GTCTCCCTGG TGTTCCAGGA TTCCCAGGAA TGAAAGGACC CATTGGAGTA 
CCAGGCTCAA CTGGCCCTGA TGGGGAACCA GGTCTTACGG GGCCCCCAGG TCCTCCTGGG 
TTGCCTGGTC CTTCAGGACA GAGTATTGTA ATCAAAGGAG ATCCTGGACC TCCAGGAATT 
CCTGGACAAC CTGGATTAAA AGGTCTACCA GGACTTCCAG GCCCTCAAGG TCTACCAGGT 
CCAATTGGCC CTCCAGGAGA TCCAGGACGC AATGGACTCC CAGGCTTTGA TGGTGCAGGA 
GGGCGCAAAG GAGACCCAGG CCTGCCGGGG CAGCCAGGTA GCCGTGGATT GGATGGTCCC 
CCAGGACCAG ATGGATTGCA AGGACCTCCA GGGCCCCCGG GAACCACCTC TGTTGCCCAT 
GGATTCCTCA TCACACGTCA CAGTCAGACA ACAGATGCAC CACAATGCCC ACAGGGAACA 
GTCCATATCT ATGATGGCTT TTCTCTCTTA TATGTACAAG GAAATAAAAG AGCCCATGGT 
CAAGACTTGG GGACAGCTGG CAGCTGCCTT CGTCGCTTCA GTACCATGCC TTTCATGTTC 
TGCAACATTA ACAACGTTTG TAACTTTGCT TCAAGAAATG ACTATTCTTA CTGGCTTTCC 
ACCCCAGAGC CCATGCCAAT GAACATGGAA CCCCTGAAGG GCCAGAGCAT CCAGCCATTC 
ATTAGTCGAT GTGCAGTATG TGAGGCACCA GCTGTGGTGA TTGCAGTTCA CAGCCAGACT 
ATTCAGATTC CTCACTGTCC TCAGGGGTGG GACTCTCTGT GGATTGGCTA TTCATTCATG 
ATGCATACAA GTGCAGGAGC AGAAGGCTCT GGCCAAGCCC TAGCCTCTCC TGGCTCCTGT 
TTGGAAGAAT TTCGTTCAGC TCCCTTCATC GAATGTCATG GACGAGGGAC ATGCAATTAC 
TATGCCAATT CTTACAGCTT TTGGCTGGCC ACTGTTGATA TGTCAGACAT GTTCAGTAAA 
CCTCAGTCAG AGACACTGAA AGCAGGCGAC TTGAGGACAC GTATTAGCCG ATGTCAAGTA 
TGCATGAAAA GGACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006257375.1
CDS	400..5457
Translation	MQVPGVCLAAGWFLLALCLWGQPGEAAACHGCSSGSKCDCSGIKGEKGERGFPGLEGHPGLPGFPGPEGPPGPRGQKGDDGIPGPPGPKGIRGPPGLPGFPGTPGLPGMPGHDGAPGPQGIPGCNGTKGERGFPGSPGFPGLQGPPGPPGIPGMKGEPGSIIMSSLPGPKGNQGYPGPPGIQGPPGPKGVSGPSGPPGPPGLMGPPGPPGPPGPKGNMGLNFQGPKGEKGEQGLQGPPGPPGQISEQKRPIDLDFQKGDQGVPGDRGPPGPPGIPGPPGPPGGVKGEKGEPGEPGKRGKPGKDGETGQPGIPGLPGDPGYPGEPGRHGEKGQKGDTGPPGPPGFVIPTPGTGVTIGAKGNIGQPGLPGEKGERGFPGIQGPPGFPGPPGTAVMGPPGPPGYPGERGQKGDEGPPGICIPGSPGLDGQPGAPGFPGPPGPPGPQLPPSDEICKAGPPGPPGSPGDQGLQGEQGVKGDKGDTCFNCIGTGISGPPGQPGLPGLPGPPGSLGIPGEKGEKGQAGTTGPKGLPGIPGPPGAPGFPGSKGDPGDVLTLPGMKGEKGELGSPGAPGLPGLPGSPGKDGLPGLPGPKGEPGGITFKGERGPPGNPGLPGLPGNMGPTGPVGFGPPGPVGEKGIQGVAGNPGQPGLPGPKGDPGQTISQPGKPGLPGNPGRDGEVGLPGDPGLPGQPGLPGIPGSKGEPGIPGIGPPGPPGPKGFPGIPGPPGPPGAPGRMGPEGPPGLPGFPGPKGEPGFGLPGPPGPPGFPGFKGTPGPKGDRGLPGPPGPPGHTGLDGLPGPKGDAGPNGQPGPIGPPGLPGIGLQGPPGPPGTPGPIGQPGLHGIPGEKGDPGPPGLDVPGLPGERGSPGLPGAPGLIGPPGSPGIPGRAGVPGFPGVKGEMGMMGPPGPPGPLGTPGRSGAPGLKGDDGMQGQPGLPGPVGEKGGKGEPGLPGPPGPMRPDFLGSKGEKGDPGLPGIPGLSGPKGYQGLPGDPGQPGLSGQPGSPGLPGPKGTPGLPGPPGLTGPPGPKGNIGDMGFPGPQGVDGPPGPPGGPGQPGSPGLPGQKGSKGEPGVSGIGLPGLPGPKGEPGLPGYPGNPGIKGSMGEAGLPGLPGSPGAKGQPGLPGFPGTPGLPGPKGMNGPPGNPGLPGEPGPVGGGGRPGPPGPPGEKGKPGQDGIPGPAGQKGEPGQPGFGIPGPPGLPGLSGQKGDGGAPGLPGNPGLPGSKGEPGFQGFPGMPGPPGPPGSPGPSLEGPKGNPGPQGPPGRPGPPGTEGPRGPPGNGGIKGERGNPGPPGQPGLPGLKGDQGPPGLPGNPGRPGLNGMKGDPGLPGVPGFPGMKGPIGVPGSTGPDGEPGLTGPPGPPGLPGPSGQSIVIKGDPGPPGIPGQPGLKGLPGLPGPQGLPGPIGPPGDPGRNGLPGFDGAGGRKGDPGLPGQPGSRGLDGPPGPDGLQGPPGPPGTTSVAHGFLITRHSQTTDAPQCPQGTVHIYDGFSLLYVQGNKRAHGQDLGTAGSCLRRFSTMPFMFCNINNVCNFASRNDYSYWLSTPEPMPMNMEPLKGQSIQPFISRCAVCEAPAVVIAVHSQTIQIPHCPQGWDSLWIGYSFMMHTSAGAEGSGQALASPGSCLEEFRSAPFIECHGRGTCNYYANSYSFWLATVDMSDMFSKPQSETLKAGDLRTRISRCQVCMKRT

Target ORF information:

RefSeq Version	XM_006257313.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus collagen type IV alpha 5 chain (Col4a5), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006257313.1

ORF Insert Sequence:

ATGCAAGTGC CTGGAGTGTG CCTGGCCGCC GGCTGGTTCT TACTGGCCCT GTGTCTGTGG 
GGGCAGCCGG GAGAGGCTGC GGCCTGCCAT GGGTGTTCTT CTGGATCAAA ATGTGACTGC 
AGTGGTATAA AAGGAGAAAA GGGAGAACGG GGCTTTCCAG GCTTAGAAGG CCATCCAGGT 
TTACCAGGAT TTCCAGGTCC AGAAGGGCCT CCAGGACCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGA ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGCCTTCC TGGGATGCCA GGCCACGATG GGGCTCCAGG ACCTCAAGGT 
ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGCGGATTTC CAGGCAGTCC AGGCTTTCCC 
GGTTTACAAG GACCTCCAGG ACCACCTGGG ATCCCAGGTA TGAAGGGGGA GCCAGGCAGT 
ATAATCATGT CATCTCTGCC AGGGCCAAAG GGTAATCAAG GATACCCAGG TCCTCCTGGA 
ATACAAGGTC CACCTGGTCC CAAGGGTGTA TCAGGGCCAA GTGGTCCTCC AGGACCACCA 
GGTTTAATGG GCCCTCCTGG CCCACCAGGC CCACCGGGAC CAAAGGGGAA TATGGGCTTA 
AATTTCCAGG GACCCAAAGG CGAAAAAGGT GAACAAGGTC TTCAGGGTCC ACCTGGTCCA 
CCTGGGCAGA TCAGTGAACA GAAAAGACCA ATTGACCTAG ATTTTCAGAA AGGCGATCAG 
GGAGTTCCCG GTGATAGAGG GCCTCCTGGA CCTCCGGGAA TACCTGGTCC TCCCGGTCCT 
CCTGGTGGTG TGAAAGGTGA AAAGGGTGAG CCCGGAGAGC CAGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAGACTGG TCAACCAGGA ATCCCAGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGTGAAC CAGGAAGGCA TGGTGAAAAG GGCCAAAAGG GTGATACTGG CCCACCTGGA 
CCACCTGGAT TTGTAATTCC TACACCTGGA ACTGGTGTGA CTATTGGAGC AAAAGGAAAT 
ATTGGGCAAC CTGGTTTGCC TGGAGAAAAA GGAGAGCGAG GATTTCCTGG CATACAAGGT 
CCACCTGGCT TTCCTGGACC TCCAGGGACA GCTGTCATGG GTCCCCCTGG TCCTCCTGGA 
TATCCTGGTG AAAGGGGCCA GAAAGGTGAT GAAGGTCCCC CTGGAATTTG TATTCCTGGA 
TCTCCTGGAC TTGATGGACA GCCTGGAGCT CCTGGCTTTC CAGGACCTCC TGGTCCTCCT 
GGCCCCCAGT TACCCCCGAG TGATGAAATC TGTAAAGCAG GCCCTCCAGG GCCCCCAGGA 
TCTCCAGGTG ACCAAGGACT CCAAGGAGAG CAAGGAGTAA AAGGTGACAA AGGTGATACT 
TGCTTCAACT GTATTGGAAC TGGCATTTCA GGGCCTCCAG GCCAACCAGG TTTACCAGGT 
CTCCCAGGTC CTCCAGGATC TCTTGGAATC CCTGGAGAGA AGGGCGAGAA AGGACAAGCT 
GGGACAACTG GTCCAAAAGG ATTGCCAGGC ATTCCTGGAC CTCCAGGTGC TCCAGGCTTT 
CCAGGGTCTA AGGGTGACCC TGGTGATGTT CTCACTCTTC CGGGAATGAA AGGTGAAAAA 
GGAGAATTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTC TACCTGGTAG TCCTGGAAAG 
GATGGTTTGC CAGGACTTCC TGGCCCCAAA GGAGAGCCCG GTGGAATCAC TTTTAAGGGT 
GAAAGGGGTC CTCCTGGGAA CCCAGGTTTA CCAGGTCTCC CAGGAAATAT GGGACCTACA 
GGCCCTGTTG GTTTTGGGCC TCCAGGCCCC GTAGGTGAAA AAGGCATACA AGGTGTGGCG 
GGAAATCCAG GCCAGCCAGG ATTACCAGGT CCTAAAGGTG ATCCAGGCCA GACCATCAGC 
CAGCCAGGAA AGCCTGGCTT GCCAGGGAAT CCAGGCAGAG ATGGTGAAGT GGGTCTTCCA 
GGTGATCCTG GACTTCCAGG CCAACCAGGC CTACCAGGAA TACCTGGTAG CAAAGGAGAA 
CCAGGTATCC CTGGGATTGG GCCTCCAGGA CCACCTGGTC CCAAAGGTTT TCCAGGAATT 
CCTGGACCTC CAGGACCTCC TGGGGCCCCT GGAAGAATGG GCCCAGAAGG GCCTCCTGGG 
TTACCGGGCT TTCCAGGACC CAAGGGAGAG CCAGGATTTG GGTTACCTGG GCCACCTGGG 
CCACCAGGAT TCCCAGGTTT CAAAGGAACA CCTGGTCCAA AAGGTGATCG TGGTTTACCA 
GGACCTCCAG GTCCTCCAGG ACACACTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAT 
GCTGGACCAA ATGGACAACC TGGCCCAATA GGGCCACCAG GGCTGCCAGG AATTGGTCTT 
CAGGGACCAC CAGGACCACC AGGGACTCCA GGGCCAATAG GCCAACCTGG CTTACATGGA 
ATACCAGGAG AGAAGGGAGA TCCAGGACCT CCTGGGCTTG ATGTTCCAGG ACTCCCTGGA 
GAGAGAGGTA GTCCAGGGCT CCCTGGAGCC CCTGGTCTTA TAGGACCTCC AGGATCACCT 
GGTATCCCTG GAAGAGCAGG TGTCCCTGGA TTTCCAGGTG TCAAAGGCGA AATGGGTATG 
ATGGGACCTC CAGGCCCACC GGGACCTTTG GGTACTCCTG GCAGGAGTGG TGCTCCTGGT 
CTTAAAGGTG ATGACGGCAT GCAAGGTCAG CCAGGTCTCC CTGGCCCTGT AGGAGAAAAG 
GGTGGTAAGG GAGAACCTGG CCTTCCAGGT CCTCCTGGAC CAATGCGTCC AGATTTCCTG 
GGATCAAAAG GAGAAAAGGG TGATCCTGGC TTACCAGGTA TTCCTGGACT TTCCGGACCA 
AAAGGCTACC AGGGATTACC TGGAGACCCA GGGCAACCTG GGCTGAGTGG ACAGCCGGGA 
TCACCAGGAC TACCAGGTCC CAAAGGTACT CCTGGTCTCC CTGGTCCACC AGGACTTACC 
GGACCTCCTG GACCTAAAGG AAACATTGGC GACATGGGTT TTCCTGGCCC TCAAGGTGTA 
GATGGGCCTC CTGGACCTCC TGGAGGTCCT GGACAACCTG GCTCTCCCGG GTTGCCTGGC 
CAGAAAGGAA GTAAAGGAGA ACCTGGAGTT TCAGGCATTG GTCTCCCTGG TCTTCCTGGT 
CCAAAGGGTG AACCTGGTCT GCCTGGATAT CCAGGAAACC CAGGTATCAA AGGTTCTATG 
GGCGAGGCTG GTCTGCCTGG CTTACCAGGA AGCCCTGGAG CAAAAGGTCA ACCCGGCCTG 
CCCGGATTCC CAGGAACACC AGGGCTTCCT GGACCAAAGG GTATGAATGG TCCTCCTGGT 
AACCCTGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGGCGTCC TGGACCACCA 
GGACCTCCTG GTGAAAAAGG CAAGCCAGGT CAAGATGGCA TTCCTGGACC AGCAGGACAA 
AAGGGAGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CTCCTGGACT CCCAGGACTT 
TCTGGTCAAA AGGGTGATGG AGGAGCCCCT GGCCTTCCAG GAAATCCTGG CCTTCCAGGT 
TCAAAGGGTG AACCAGGCTT TCAGGGATTC CCTGGTATGC CAGGTCCCCC AGGTCCCCCT 
GGTTCTCCTG GCCCATCTTT GGAAGGTCCC AAAGGAAACC CTGGGCCTCA AGGCCCTCCT 
GGGAGACCAG GTCCACCTGG TACAGAAGGT CCTCGTGGCC CCCCTGGAAA TGGAGGTATT 
AAAGGAGAGA GGGGAAATCC AGGTCCACCT GGGCAACCAG GCTTACCTGG CTTGAAAGGA 
GATCAAGGAC CACCAGGCCT TCCGGGTAAC CCTGGCCGAC CAGGTCTTAA TGGAATGAAA 
GGAGATCCTG GTCTCCCTGG TGTTCCAGGA TTCCCAGGAA TGAAAGGACC CATTGGAGTA 
CCAGGCTCAA CTGGCCCTGA TGGGGAACCA GGTCTTACGG GGCCCCCAGG TCCTCCTGGG 
TTGCCTGGTC CTTCAGGACA GAGTATTGTA ATCAAAGGAG ATCCTGGACC TCCAGGAATT 
CCTGGACAAC CTGGATTAAA AGGTCTACCA GGACTTCCAG GCCCTCAAGG TCTACCAGGT 
CCAATTGGCC CTCCAGGAGA TCCAGGACGC AATGGACTCC CAGGCTTTGA TGGTGCAGGA 
GGGCGCAAAG GAGACCCAGG CCTGCCGGGG CAGCCAGGTA GCCGTGGATT GGATGGTCCC 
CCAGGACCAG ATGGATTGCA AGGACCTCCA GGGCCCCCGG GAACCACCTC TGTTGCCCAT 
GGATTCCTCA TCACACGTCA CAGTCAGACA ACAGATGCAC CACAATGCCC ACAGGGAACA 
GTCCATATCT ATGATGGCTT TTCTCTCTTA TATGTACAAG GAAATAAAAG AGCCCATGGT 
CAAGACTTGG GGACAGCTGG CAGCTGCCTT CGTCGCTTCA GTACCATGCC TTTCATGTTC 
TGCAACATTA ACAACGTTTG TAACTTTGCT TCAAGAAATG ACTATTCTTA CTGGCTTTCC 
ACCCCAGAGC CCATGCCAAT GAACATGGAA CCCCTGAAGG GCCAGAGCAT CCAGCCATTC 
ATTAGTCGAT GTGCAGTATG TGAGGCACCA GCTGTGGTGA TTGCAGTTCA CAGCCAGACT 
ATTCAGATTC CTCACTGTCC TCAGGGGTGG GACTCTCTGT GGATTGGCTA TTCATTCATG 
ATGCATACAA GTGCAGGAGC AGAAGGCTCT GGCCAAGCCC TAGCCTCTCC TGGCTCCTGT 
TTGGAAGAAT TTCGTTCAGC TCCCTTCATC GAATGTCATG GACGAGGGAC ATGCAATTAC 
TATGCCAATT CTTACAGCTT TTGGCTGGCC ACTGTTGATA TGTCAGACAT GTTCAGTAAA 
CCTCAGTCAG AGACACTGAA AGCAGGCGAC TTGAGGACAC GTATTAGCCG ATGTCAAGTA 
TGCATGAAAA GGACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col4a5 ( XM_006227491.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006227553.1 Rattus norvegicus collagen type IV alpha 5 chain (Col4a5), transcript variant X4, mRNA.

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CloneID	ORa21619
Clone ID Related Accession (Same CDS sequence)	XM_006257313.1 , XM_006227491.1
Accession Version	XM_006227491.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	collagen alpha-5(IV) chain isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000089.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGTGC CTGGAGTGTG CCTGGCCGCC GGCTGGTTCT TACTGGCCCT GTGTCTGTGG 
GGGCAGCCGG GAGAGGCTGC GGCCTGCCAT GGGTGTTCTT CTGGATCAAA ATGTGACTGC 
AGTGGTATAA AAGGAGAAAA GGGAGAACGG GGCTTTCCAG GCTTAGAAGG CCATCCAGGT 
TTACCAGGAT TTCCAGGTCC AGAAGGGCCT CCAGGACCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGA ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGCCTTCC TGGGATGCCA GGCCACGATG GGGCTCCAGG ACCTCAAGGT 
ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGCGGATTTC CAGGCAGTCC AGGCTTTCCC 
GGTTTACAAG GACCTCCAGG ACCACCTGGG ATCCCAGGTA TGAAGGGGGA GCCAGGCAGT 
ATAATCATGT CATCTCTGCC AGGGCCAAAG GGTAATCAAG GATACCCAGG TCCTCCTGGA 
ATACAAGGTC CACCTGGTCC CAAGGGTGTA TCAGGGCCAA GTGGTCCTCC AGGACCACCA 
GGTTTAATGG GCCCTCCTGG CCCACCAGGC CCACCGGGAC CAAAGGGGAA TATGGGCTTA 
AATTTCCAGG GACCCAAAGG CGAAAAAGGT GAACAAGGTC TTCAGGGTCC ACCTGGTCCA 
CCTGGGCAGA TCAGTGAACA GAAAAGACCA ATTGACCTAG ATTTTCAGAA AGGCGATCAG 
GGAGTTCCCG GTGATAGAGG GCCTCCTGGA CCTCCGGGAA TACCTGGTCC TCCCGGTCCT 
CCTGGTGGTG TGAAAGGTGA AAAGGGTGAG CCCGGAGAGC CAGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAGACTGG TCAACCAGGA ATCCCAGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGTGAAC CAGGAAGGCA TGGTGAAAAG GGCCAAAAGG GTGATACTGG CCCACCTGGA 
CCACCTGGAT TTGTAATTCC TACACCTGGA ACTGGTGTGA CTATTGGAGC AAAAGGAAAT 
ATTGGGCAAC CTGGTTTGCC TGGAGAAAAA GGAGAGCGAG GATTTCCTGG CATACAAGGT 
CCACCTGGCT TTCCTGGACC TCCAGGGACA GCTGTCATGG GTCCCCCTGG TCCTCCTGGA 
TATCCTGGTG AAAGGGGCCA GAAAGGTGAT GAAGGTCCCC CTGGAATTTG TATTCCTGGA 
TCTCCTGGAC TTGATGGACA GCCTGGAGCT CCTGGCTTTC CAGGACCTCC TGGTCCTCCT 
GGCCCCCAGT TACCCCCGAG TGATGAAATC TGTAAAGCAG GCCCTCCAGG GCCCCCAGGA 
TCTCCAGGTG ACCAAGGACT CCAAGGAGAG CAAGGAGTAA AAGGTGACAA AGGTGATACT 
TGCTTCAACT GTATTGGAAC TGGCATTTCA GGGCCTCCAG GCCAACCAGG TTTACCAGGT 
CTCCCAGGTC CTCCAGGATC TCTTGGAATC CCTGGAGAGA AGGGCGAGAA AGGACAAGCT 
GGGACAACTG GTCCAAAAGG ATTGCCAGGC ATTCCTGGAC CTCCAGGTGC TCCAGGCTTT 
CCAGGGTCTA AGGGTGACCC TGGTGATGTT CTCACTCTTC CGGGAATGAA AGGTGAAAAA 
GGAGAATTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTC TACCTGGTAG TCCTGGAAAG 
GATGGTTTGC CAGGACTTCC TGGCCCCAAA GGAGAGCCCG GTGGAATCAC TTTTAAGGGT 
GAAAGGGGTC CTCCTGGGAA CCCAGGTTTA CCAGGTCTCC CAGGAAATAT GGGACCTACA 
GGCCCTGTTG GTTTTGGGCC TCCAGGCCCC GTAGGTGAAA AAGGCATACA AGGTGTGGCG 
GGAAATCCAG GCCAGCCAGG ATTACCAGGT CCTAAAGGTG ATCCAGGCCA GACCATCAGC 
CAGCCAGGAA AGCCTGGCTT GCCAGGGAAT CCAGGCAGAG ATGGTGAAGT GGGTCTTCCA 
GGTGATCCTG GACTTCCAGG CCAACCAGGC CTACCAGGAA TACCTGGTAG CAAAGGAGAA 
CCAGGTATCC CTGGGATTGG GCCTCCAGGA CCACCTGGTC CCAAAGGTTT TCCAGGAATT 
CCTGGACCTC CAGGACCTCC TGGGGCCCCT GGAAGAATGG GCCCAGAAGG GCCTCCTGGG 
TTACCGGGCT TTCCAGGACC CAAGGGAGAG CCAGGATTTG GGTTACCTGG GCCACCTGGG 
CCACCAGGAT TCCCAGGTTT CAAAGGAACA CCTGGTCCAA AAGGTGATCG TGGTTTACCA 
GGACCTCCAG GTCCTCCAGG ACACACTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAT 
GCTGGACCAA ATGGACAACC TGGCCCAATA GGGCCACCAG GGCTGCCAGG AATTGGTCTT 
CAGGGACCAC CAGGACCACC AGGGACTCCA GGGCCAATAG GCCAACCTGG CTTACATGGA 
ATACCAGGAG AGAAGGGAGA TCCAGGACCT CCTGGGCTTG ATGTTCCAGG ACTCCCTGGA 
GAGAGAGGTA GTCCAGGGCT CCCTGGAGCC CCTGGTCTTA TAGGACCTCC AGGATCACCT 
GGTATCCCTG GAAGAGCAGG TGTCCCTGGA TTTCCAGGTG TCAAAGGCGA AATGGGTATG 
ATGGGACCTC CAGGCCCACC GGGACCTTTG GGTACTCCTG GCAGGAGTGG TGCTCCTGGT 
CTTAAAGGTG ATGACGGCAT GCAAGGTCAG CCAGGTCTCC CTGGCCCTGT AGGAGAAAAG 
GGTGGTAAGG GAGAACCTGG CCTTCCAGGT CCTCCTGGAC CAATGCGTCC AGATTTCCTG 
GGATCAAAAG GAGAAAAGGG TGATCCTGGC TTACCAGGTA TTCCTGGACT TTCCGGACCA 
AAAGGCTACC AGGGATTACC TGGAGACCCA GGGCAACCTG GGCTGAGTGG ACAGCCGGGA 
TCACCAGGAC TACCAGGTCC CAAAGGTACT CCTGGTCTCC CTGGTCCACC AGGACTTACC 
GGACCTCCTG GACCTAAAGG AAACATTGGC GACATGGGTT TTCCTGGCCC TCAAGGTGTA 
GATGGGCCTC CTGGACCTCC TGGAGGTCCT GGACAACCTG GCTCTCCCGG GTTGCCTGGC 
CAGAAAGGAA GTAAAGGAGA ACCTGGAGTT TCAGGCATTG GTCTCCCTGG TCTTCCTGGT 
CCAAAGGGTG AACCTGGTCT GCCTGGATAT CCAGGAAACC CAGGTATCAA AGGTTCTATG 
GGCGAGGCTG GTCTGCCTGG CTTACCAGGA AGCCCTGGAG CAAAAGGTCA ACCCGGCCTG 
CCCGGATTCC CAGGAACACC AGGGCTTCCT GGACCAAAGG GTATGAATGG TCCTCCTGGT 
AACCCTGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGGCGTCC TGGACCACCA 
GGACCTCCTG GTGAAAAAGG CAAGCCAGGT CAAGATGGCA TTCCTGGACC AGCAGGACAA 
AAGGGAGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CTCCTGGACT CCCAGGACTT 
TCTGGTCAAA AGGGTGATGG AGGAGCCCCT GGCCTTCCAG GAAATCCTGG CCTTCCAGGT 
TCAAAGGGTG AACCAGGCTT TCAGGGATTC CCTGGTATGC CAGGTCCCCC AGGTCCCCCT 
GGTTCTCCTG GCCCATCTTT GGAAGGTCCC AAAGGAAACC CTGGGCCTCA AGGCCCTCCT 
GGGAGACCAG GTCCACCTGG TACAGAAGGT CCTCGTGGCC CCCCTGGAAA TGGAGGTATT 
AAAGGAGAGA GGGGAAATCC AGGTCCACCT GGGCAACCAG GCTTACCTGG CTTGAAAGGA 
GATCAAGGAC CACCAGGCCT TCCGGGTAAC CCTGGCCGAC CAGGTCTTAA TGGAATGAAA 
GGAGATCCTG GTCTCCCTGG TGTTCCAGGA TTCCCAGGAA TGAAAGGACC CATTGGAGTA 
CCAGGCTCAA CTGGCCCTGA TGGGGAACCA GGTCTTACGG GGCCCCCAGG TCCTCCTGGG 
TTGCCTGGTC CTTCAGGACA GAGTATTGTA ATCAAAGGAG ATCCTGGACC TCCAGGAATT 
CCTGGACAAC CTGGATTAAA AGGTCTACCA GGACTTCCAG GCCCTCAAGG TCTACCAGGT 
CCAATTGGCC CTCCAGGAGA TCCAGGACGC AATGGACTCC CAGGCTTTGA TGGTGCAGGA 
GGGCGCAAAG GAGACCCAGG CCTGCCGGGG CAGCCAGGTA GCCGTGGATT GGATGGTCCC 
CCAGGACCAG ATGGATTGCA AGGACCTCCA GGGCCCCCGG GAACCACCTC TGTTGCCCAT 
GGATTCCTCA TCACACGTCA CAGTCAGACA ACAGATGCAC CACAATGCCC ACAGGGAACA 
GTCCATATCT ATGATGGCTT TTCTCTCTTA TATGTACAAG GAAATAAAAG AGCCCATGGT 
CAAGACTTGG GGACAGCTGG CAGCTGCCTT CGTCGCTTCA GTACCATGCC TTTCATGTTC 
TGCAACATTA ACAACGTTTG TAACTTTGCT TCAAGAAATG ACTATTCTTA CTGGCTTTCC 
ACCCCAGAGC CCATGCCAAT GAACATGGAA CCCCTGAAGG GCCAGAGCAT CCAGCCATTC 
ATTAGTCGAT GTGCAGTATG TGAGGCACCA GCTGTGGTGA TTGCAGTTCA CAGCCAGACT 
ATTCAGATTC CTCACTGTCC TCAGGGGTGG GACTCTCTGT GGATTGGCTA TTCATTCATG 
ATGCATACAA GTGCAGGAGC AGAAGGCTCT GGCCAAGCCC TAGCCTCTCC TGGCTCCTGT 
TTGGAAGAAT TTCGTTCAGC TCCCTTCATC GAATGTCATG GACGAGGGAC ATGCAATTAC 
TATGCCAATT CTTACAGCTT TTGGCTGGCC ACTGTTGATA TGTCAGACAT GTTCAGTAAA 
CCTCAGTCAG AGACACTGAA AGCAGGCGAC TTGAGGACAC GTATTAGCCG ATGTCAAGTA 
TGCATGAAAA GGACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006227553.1
CDS	400..5457
Translation	MQVPGVCLAAGWFLLALCLWGQPGEAAACHGCSSGSKCDCSGIKGEKGERGFPGLEGHPGLPGFPGPEGPPGPRGQKGDDGIPGPPGPKGIRGPPGLPGFPGTPGLPGMPGHDGAPGPQGIPGCNGTKGERGFPGSPGFPGLQGPPGPPGIPGMKGEPGSIIMSSLPGPKGNQGYPGPPGIQGPPGPKGVSGPSGPPGPPGLMGPPGPPGPPGPKGNMGLNFQGPKGEKGEQGLQGPPGPPGQISEQKRPIDLDFQKGDQGVPGDRGPPGPPGIPGPPGPPGGVKGEKGEPGEPGKRGKPGKDGETGQPGIPGLPGDPGYPGEPGRHGEKGQKGDTGPPGPPGFVIPTPGTGVTIGAKGNIGQPGLPGEKGERGFPGIQGPPGFPGPPGTAVMGPPGPPGYPGERGQKGDEGPPGICIPGSPGLDGQPGAPGFPGPPGPPGPQLPPSDEICKAGPPGPPGSPGDQGLQGEQGVKGDKGDTCFNCIGTGISGPPGQPGLPGLPGPPGSLGIPGEKGEKGQAGTTGPKGLPGIPGPPGAPGFPGSKGDPGDVLTLPGMKGEKGELGSPGAPGLPGLPGSPGKDGLPGLPGPKGEPGGITFKGERGPPGNPGLPGLPGNMGPTGPVGFGPPGPVGEKGIQGVAGNPGQPGLPGPKGDPGQTISQPGKPGLPGNPGRDGEVGLPGDPGLPGQPGLPGIPGSKGEPGIPGIGPPGPPGPKGFPGIPGPPGPPGAPGRMGPEGPPGLPGFPGPKGEPGFGLPGPPGPPGFPGFKGTPGPKGDRGLPGPPGPPGHTGLDGLPGPKGDAGPNGQPGPIGPPGLPGIGLQGPPGPPGTPGPIGQPGLHGIPGEKGDPGPPGLDVPGLPGERGSPGLPGAPGLIGPPGSPGIPGRAGVPGFPGVKGEMGMMGPPGPPGPLGTPGRSGAPGLKGDDGMQGQPGLPGPVGEKGGKGEPGLPGPPGPMRPDFLGSKGEKGDPGLPGIPGLSGPKGYQGLPGDPGQPGLSGQPGSPGLPGPKGTPGLPGPPGLTGPPGPKGNIGDMGFPGPQGVDGPPGPPGGPGQPGSPGLPGQKGSKGEPGVSGIGLPGLPGPKGEPGLPGYPGNPGIKGSMGEAGLPGLPGSPGAKGQPGLPGFPGTPGLPGPKGMNGPPGNPGLPGEPGPVGGGGRPGPPGPPGEKGKPGQDGIPGPAGQKGEPGQPGFGIPGPPGLPGLSGQKGDGGAPGLPGNPGLPGSKGEPGFQGFPGMPGPPGPPGSPGPSLEGPKGNPGPQGPPGRPGPPGTEGPRGPPGNGGIKGERGNPGPPGQPGLPGLKGDQGPPGLPGNPGRPGLNGMKGDPGLPGVPGFPGMKGPIGVPGSTGPDGEPGLTGPPGPPGLPGPSGQSIVIKGDPGPPGIPGQPGLKGLPGLPGPQGLPGPIGPPGDPGRNGLPGFDGAGGRKGDPGLPGQPGSRGLDGPPGPDGLQGPPGPPGTTSVAHGFLITRHSQTTDAPQCPQGTVHIYDGFSLLYVQGNKRAHGQDLGTAGSCLRRFSTMPFMFCNINNVCNFASRNDYSYWLSTPEPMPMNMEPLKGQSIQPFISRCAVCEAPAVVIAVHSQTIQIPHCPQGWDSLWIGYSFMMHTSAGAEGSGQALASPGSCLEEFRSAPFIECHGRGTCNYYANSYSFWLATVDMSDMFSKPQSETLKAGDLRTRISRCQVCMKRT

Target ORF information:

RefSeq Version	XM_006227491.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus collagen type IV alpha 5 chain (Col4a5), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006227491.1

ORF Insert Sequence:

ATGCAAGTGC CTGGAGTGTG CCTGGCCGCC GGCTGGTTCT TACTGGCCCT GTGTCTGTGG 
GGGCAGCCGG GAGAGGCTGC GGCCTGCCAT GGGTGTTCTT CTGGATCAAA ATGTGACTGC 
AGTGGTATAA AAGGAGAAAA GGGAGAACGG GGCTTTCCAG GCTTAGAAGG CCATCCAGGT 
TTACCAGGAT TTCCAGGTCC AGAAGGGCCT CCAGGACCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGA ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGCCTTCC TGGGATGCCA GGCCACGATG GGGCTCCAGG ACCTCAAGGT 
ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGCGGATTTC CAGGCAGTCC AGGCTTTCCC 
GGTTTACAAG GACCTCCAGG ACCACCTGGG ATCCCAGGTA TGAAGGGGGA GCCAGGCAGT 
ATAATCATGT CATCTCTGCC AGGGCCAAAG GGTAATCAAG GATACCCAGG TCCTCCTGGA 
ATACAAGGTC CACCTGGTCC CAAGGGTGTA TCAGGGCCAA GTGGTCCTCC AGGACCACCA 
GGTTTAATGG GCCCTCCTGG CCCACCAGGC CCACCGGGAC CAAAGGGGAA TATGGGCTTA 
AATTTCCAGG GACCCAAAGG CGAAAAAGGT GAACAAGGTC TTCAGGGTCC ACCTGGTCCA 
CCTGGGCAGA TCAGTGAACA GAAAAGACCA ATTGACCTAG ATTTTCAGAA AGGCGATCAG 
GGAGTTCCCG GTGATAGAGG GCCTCCTGGA CCTCCGGGAA TACCTGGTCC TCCCGGTCCT 
CCTGGTGGTG TGAAAGGTGA AAAGGGTGAG CCCGGAGAGC CAGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAGACTGG TCAACCAGGA ATCCCAGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGTGAAC CAGGAAGGCA TGGTGAAAAG GGCCAAAAGG GTGATACTGG CCCACCTGGA 
CCACCTGGAT TTGTAATTCC TACACCTGGA ACTGGTGTGA CTATTGGAGC AAAAGGAAAT 
ATTGGGCAAC CTGGTTTGCC TGGAGAAAAA GGAGAGCGAG GATTTCCTGG CATACAAGGT 
CCACCTGGCT TTCCTGGACC TCCAGGGACA GCTGTCATGG GTCCCCCTGG TCCTCCTGGA 
TATCCTGGTG AAAGGGGCCA GAAAGGTGAT GAAGGTCCCC CTGGAATTTG TATTCCTGGA 
TCTCCTGGAC TTGATGGACA GCCTGGAGCT CCTGGCTTTC CAGGACCTCC TGGTCCTCCT 
GGCCCCCAGT TACCCCCGAG TGATGAAATC TGTAAAGCAG GCCCTCCAGG GCCCCCAGGA 
TCTCCAGGTG ACCAAGGACT CCAAGGAGAG CAAGGAGTAA AAGGTGACAA AGGTGATACT 
TGCTTCAACT GTATTGGAAC TGGCATTTCA GGGCCTCCAG GCCAACCAGG TTTACCAGGT 
CTCCCAGGTC CTCCAGGATC TCTTGGAATC CCTGGAGAGA AGGGCGAGAA AGGACAAGCT 
GGGACAACTG GTCCAAAAGG ATTGCCAGGC ATTCCTGGAC CTCCAGGTGC TCCAGGCTTT 
CCAGGGTCTA AGGGTGACCC TGGTGATGTT CTCACTCTTC CGGGAATGAA AGGTGAAAAA 
GGAGAATTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTC TACCTGGTAG TCCTGGAAAG 
GATGGTTTGC CAGGACTTCC TGGCCCCAAA GGAGAGCCCG GTGGAATCAC TTTTAAGGGT 
GAAAGGGGTC CTCCTGGGAA CCCAGGTTTA CCAGGTCTCC CAGGAAATAT GGGACCTACA 
GGCCCTGTTG GTTTTGGGCC TCCAGGCCCC GTAGGTGAAA AAGGCATACA AGGTGTGGCG 
GGAAATCCAG GCCAGCCAGG ATTACCAGGT CCTAAAGGTG ATCCAGGCCA GACCATCAGC 
CAGCCAGGAA AGCCTGGCTT GCCAGGGAAT CCAGGCAGAG ATGGTGAAGT GGGTCTTCCA 
GGTGATCCTG GACTTCCAGG CCAACCAGGC CTACCAGGAA TACCTGGTAG CAAAGGAGAA 
CCAGGTATCC CTGGGATTGG GCCTCCAGGA CCACCTGGTC CCAAAGGTTT TCCAGGAATT 
CCTGGACCTC CAGGACCTCC TGGGGCCCCT GGAAGAATGG GCCCAGAAGG GCCTCCTGGG 
TTACCGGGCT TTCCAGGACC CAAGGGAGAG CCAGGATTTG GGTTACCTGG GCCACCTGGG 
CCACCAGGAT TCCCAGGTTT CAAAGGAACA CCTGGTCCAA AAGGTGATCG TGGTTTACCA 
GGACCTCCAG GTCCTCCAGG ACACACTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAT 
GCTGGACCAA ATGGACAACC TGGCCCAATA GGGCCACCAG GGCTGCCAGG AATTGGTCTT 
CAGGGACCAC CAGGACCACC AGGGACTCCA GGGCCAATAG GCCAACCTGG CTTACATGGA 
ATACCAGGAG AGAAGGGAGA TCCAGGACCT CCTGGGCTTG ATGTTCCAGG ACTCCCTGGA 
GAGAGAGGTA GTCCAGGGCT CCCTGGAGCC CCTGGTCTTA TAGGACCTCC AGGATCACCT 
GGTATCCCTG GAAGAGCAGG TGTCCCTGGA TTTCCAGGTG TCAAAGGCGA AATGGGTATG 
ATGGGACCTC CAGGCCCACC GGGACCTTTG GGTACTCCTG GCAGGAGTGG TGCTCCTGGT 
CTTAAAGGTG ATGACGGCAT GCAAGGTCAG CCAGGTCTCC CTGGCCCTGT AGGAGAAAAG 
GGTGGTAAGG GAGAACCTGG CCTTCCAGGT CCTCCTGGAC CAATGCGTCC AGATTTCCTG 
GGATCAAAAG GAGAAAAGGG TGATCCTGGC TTACCAGGTA TTCCTGGACT TTCCGGACCA 
AAAGGCTACC AGGGATTACC TGGAGACCCA GGGCAACCTG GGCTGAGTGG ACAGCCGGGA 
TCACCAGGAC TACCAGGTCC CAAAGGTACT CCTGGTCTCC CTGGTCCACC AGGACTTACC 
GGACCTCCTG GACCTAAAGG AAACATTGGC GACATGGGTT TTCCTGGCCC TCAAGGTGTA 
GATGGGCCTC CTGGACCTCC TGGAGGTCCT GGACAACCTG GCTCTCCCGG GTTGCCTGGC 
CAGAAAGGAA GTAAAGGAGA ACCTGGAGTT TCAGGCATTG GTCTCCCTGG TCTTCCTGGT 
CCAAAGGGTG AACCTGGTCT GCCTGGATAT CCAGGAAACC CAGGTATCAA AGGTTCTATG 
GGCGAGGCTG GTCTGCCTGG CTTACCAGGA AGCCCTGGAG CAAAAGGTCA ACCCGGCCTG 
CCCGGATTCC CAGGAACACC AGGGCTTCCT GGACCAAAGG GTATGAATGG TCCTCCTGGT 
AACCCTGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGGCGTCC TGGACCACCA 
GGACCTCCTG GTGAAAAAGG CAAGCCAGGT CAAGATGGCA TTCCTGGACC AGCAGGACAA 
AAGGGAGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CTCCTGGACT CCCAGGACTT 
TCTGGTCAAA AGGGTGATGG AGGAGCCCCT GGCCTTCCAG GAAATCCTGG CCTTCCAGGT 
TCAAAGGGTG AACCAGGCTT TCAGGGATTC CCTGGTATGC CAGGTCCCCC AGGTCCCCCT 
GGTTCTCCTG GCCCATCTTT GGAAGGTCCC AAAGGAAACC CTGGGCCTCA AGGCCCTCCT 
GGGAGACCAG GTCCACCTGG TACAGAAGGT CCTCGTGGCC CCCCTGGAAA TGGAGGTATT 
AAAGGAGAGA GGGGAAATCC AGGTCCACCT GGGCAACCAG GCTTACCTGG CTTGAAAGGA 
GATCAAGGAC CACCAGGCCT TCCGGGTAAC CCTGGCCGAC CAGGTCTTAA TGGAATGAAA 
GGAGATCCTG GTCTCCCTGG TGTTCCAGGA TTCCCAGGAA TGAAAGGACC CATTGGAGTA 
CCAGGCTCAA CTGGCCCTGA TGGGGAACCA GGTCTTACGG GGCCCCCAGG TCCTCCTGGG 
TTGCCTGGTC CTTCAGGACA GAGTATTGTA ATCAAAGGAG ATCCTGGACC TCCAGGAATT 
CCTGGACAAC CTGGATTAAA AGGTCTACCA GGACTTCCAG GCCCTCAAGG TCTACCAGGT 
CCAATTGGCC CTCCAGGAGA TCCAGGACGC AATGGACTCC CAGGCTTTGA TGGTGCAGGA 
GGGCGCAAAG GAGACCCAGG CCTGCCGGGG CAGCCAGGTA GCCGTGGATT GGATGGTCCC 
CCAGGACCAG ATGGATTGCA AGGACCTCCA GGGCCCCCGG GAACCACCTC TGTTGCCCAT 
GGATTCCTCA TCACACGTCA CAGTCAGACA ACAGATGCAC CACAATGCCC ACAGGGAACA 
GTCCATATCT ATGATGGCTT TTCTCTCTTA TATGTACAAG GAAATAAAAG AGCCCATGGT 
CAAGACTTGG GGACAGCTGG CAGCTGCCTT CGTCGCTTCA GTACCATGCC TTTCATGTTC 
TGCAACATTA ACAACGTTTG TAACTTTGCT TCAAGAAATG ACTATTCTTA CTGGCTTTCC 
ACCCCAGAGC CCATGCCAAT GAACATGGAA CCCCTGAAGG GCCAGAGCAT CCAGCCATTC 
ATTAGTCGAT GTGCAGTATG TGAGGCACCA GCTGTGGTGA TTGCAGTTCA CAGCCAGACT 
ATTCAGATTC CTCACTGTCC TCAGGGGTGG GACTCTCTGT GGATTGGCTA TTCATTCATG 
ATGCATACAA GTGCAGGAGC AGAAGGCTCT GGCCAAGCCC TAGCCTCTCC TGGCTCCTGT 
TTGGAAGAAT TTCGTTCAGC TCCCTTCATC GAATGTCATG GACGAGGGAC ATGCAATTAC 
TATGCCAATT CTTACAGCTT TTGGCTGGCC ACTGTTGATA TGTCAGACAT GTTCAGTAAA 
CCTCAGTCAG AGACACTGAA AGCAGGCGAC TTGAGGACAC GTATTAGCCG ATGTCAAGTA 
TGCATGAAAA GGACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col4a5 ( XM_008758534.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008756756.1 Rattus norvegicus collagen type IV alpha 5 chain (Col4a5), transcript variant X3, mRNA.

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CloneID	ORa48575
Clone ID Related Accession (Same CDS sequence)	XM_008758534.1 , XM_008773475.1
Accession Version	XM_008758534.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5067bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	collagen alpha-5(IV) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000089.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGTGC CTGGAGTGTG CCTGGCCGCC GGCTGGTTCT TACTGGCCCT GTGTCTGTGG 
GGGCAGCCGG GAGAGGCTGC GGCCTGCCAT GGGTGTTCTT CTGGATCAAA ATGTGACTGC 
AGTGGTATAA AAGGAGAAAA GGGAGAACGG GGCTTTCCAG GCTTAGAAGG CCATCCAGGT 
TTACCAGGAT TTCCAGGTCC AGAAGGGCCT CCAGGACCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGA ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGCCTTCC TGGGATGCCA GGCCACGATG GGGCTCCAGG ACCTCAAGGT 
ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGCGGATTTC CAGGCAGTCC AGGCTTTCCC 
GGTTTACAAG GACCTCCAGG ACCACCTGGG ATCCCAGGTA TGAAGGGGGA GCCAGGCAGT 
ATAATCATGT CATCTCTGCC AGGGCCAAAG GGTAATCAAG GATACCCAGG TCCTCCTGGA 
ATACAAGGTC CACCTGGTCC CAAGGGTGTA TCAGGGCCAA GTGGTCCTCC AGGACCACCA 
GGTTTAATGG GCCCTCCTGG CCCACCAGGC CCACCGGGAC CAAAGGGGAA TATGGGCTTA 
AATTTCCAGG GACCCAAAGG CGAAAAAGGT GAACAAGGTC TTCAGGGTCC ACCTGGTCCA 
CCTGGGCAGA TCAGTGAACA GAAAAGACCA ATTGACCTAG ATTTTCAGAA AGGCGATCAG 
GGAGTTCCCG GTGATAGAGG GCCTCCTGGA CCTCCGGGAA TACCTGGTCC TCCCGGTCCT 
CCTGGTGGTG TGAAAGGTGA AAAGGGTGAG CCCGGAGAGC CAGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAGACTGG TCAACCAGGA ATCCCAGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGTGAAC CAGGAAGGCA TGGTGAAAAG GGCCAAAAGG GTGATACTGG CCCACCTGGA 
CCACCTGGAT TTGTAATTCC TACACCTGGA ACTGGTGTGA CTATTGGAGC AAAAGGAAAT 
ATTGGGCAAC CTGGTTTGCC TGGAGAAAAA GGAGAGCGAG GATTTCCTGG CATACAAGGT 
CCACCTGGCT TTCCTGGACC TCCAGGGACA GCTGTCATGG GTCCCCCTGG TCCTCCTGGA 
TATCCTGGTG AAAGGGGCCA GAAAGGTGAT GAAGGTCCCC CTGGAATTTG TATTCCTGGA 
TCTCCTGGAC TTGATGGACA GCCTGGAGCT CCTGGCTTTC CAGGACCTCC TGGTCCTCCT 
GGCCCCCAGT TACCCCCGAG TGATGAAATC TGTAAAGCAG GCCCTCCAGG GCCCCCAGGA 
TCTCCAGGTG ACCAAGGACT CCAAGGAGAG CAAGGAGTAA AAGGTGACAA AGGTGATACT 
TGCTTCAACT GTATTGGAAC TGGCATTTCA GGGCCTCCAG GCCAACCAGG TTTACCAGGT 
CTCCCAGGTC CTCCAGGATC TCTTGGAATC CCTGGAGAGA AGGGCGAGAA AGGACAAGCT 
GGGACAACTG GTCCAAAAGG ATTGCCAGGC ATTCCTGGAC CTCCAGGTGC TCCAGGCTTT 
CCAGGGTCTA AGGGTGACCC TGGTGATGTT CTCACTCTTC CGGGAATGAA AGGTGAAAAA 
GGAGAATTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTC TACCTGGTAG TCCTGGAAAG 
GATGGTTTGC CAGGACTTCC TGGCCCCAAA GGAGAGCCCG GTGGAATCAC TTTTAAGGGT 
GAAAGGGGTC CTCCTGGGAA CCCAGGTTTA CCAGGTCTCC CAGGAAATAT GGGACCTACA 
GGCCCTGTTG GTTTTGGGCC TCCAGGCCCC GTAGGTGAAA AAGGCATACA AGGTGTGGCG 
GGAAATCCAG GCCAGCCAGG ATTACCAGGT CCTAAAGGTG ATCCAGGCCA GACCATCAGC 
CAGCCAGGAA AGCCTGGCTT GCCAGGGAAT CCAGGCAGAG ATGGTGAAGT GGGTCTTCCA 
GGTGATCCTG GACTTCCAGG CCAACCAGGC CTACCAGGAA TACCTGGTAG CAAAGGAGAA 
CCAGGTATCC CTGGGATTGG GCCTCCAGGA CCACCTGGTC CCAAAGGTTT TCCAGGAATT 
CCTGGACCTC CAGGACCTCC TGGGGCCCCT GGAAGAATGG GCCCAGAAGG GCCTCCTGGG 
TTACCGGGCT TTCCAGGACC CAAGGGAGAG CCAGGATTTG GGTTACCTGG GCCACCTGGG 
CCACCAGGAT TCCCAGGTTT CAAAGGAACA CCTGGTCCAA AAGGTGATCG TGGTTTACCA 
GGACCTCCAG GTCCTCCAGG ACACACTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAT 
GCTGGACCAA ATGGACAACC TGGCCCAATA GGGCCACCAG GGCTGCCAGG AATTGGTCTT 
CAGGGACCAC CAGGACCACC AGGGACTCCA GGGCCAATAG GCCAACCTGG CTTACATGGA 
ATACCAGGAG AGAAGGGAGA TCCAGGACCT CCTGGGCTTG ATGTTCCAGG ACTCCCTGGA 
GAGAGAGGTA GTCCAGGGCT CCCTGGAGCC CCTGGTCTTA TAGGACCTCC AGGATCACCT 
GGTATCCCTG GAAGAGCAGG TGTCCCTGGA TTTCCAGGTG TCAAAGGCGA AATGGGTATG 
ATGGGACCTC CAGGCCCACC GGGACCTTTG GGTACTCCTG GCAGGAGTGG TGCTCCTGGT 
CTTAAAGGTG ATGACGGCAT GCAAGGTCAG CCAGGTCTCC CTGGCCCTGT AGGAGAAAAG 
GGTGGTAAGG GAGAACCTGG CCTTCCAGGT CCTCCTGGAC CAATGCGTCC AGATTTCCTG 
GGATCAAAAG GAGAAAAGGG TGATCCTGGC TTACCAGGTA TTCCTGGACT TTCCGGACCA 
AAAGGCTACC AGGGATTACC TGGAGACCCA GGGCAACCTG GGCTGAGTGG ACAGCCGGGA 
TCACCAGGAC TACCAGGTCC CAAAGGTACT CCTGGTCTCC CTGGTCCACC AGGACTTACC 
GGACCTCCTG GACCTAAAGG AAACATTGGC GACATGGGTT TTCCTGGCCC TCAAGGTGTA 
GATGGGCCTC CTGGACCTCC TGGAGGTCCT GGACAACCTG GCTCTCCCGG GTTGCCTGGC 
CAGAAAGGAA GTAAAGGAGA ACCTGGAGTT TCAGGCATTG GTCTCCCTGG TCTTCCTGGT 
CCAAAGGGTG AACCTGGTCT GCCTGGATAT CCAGGAAACC CAGGTATCAA AGGTTCTATG 
GGCGAGGCTG GTCTGCCTGG CTTACCAGGA AGCCCTGGAG CAAAAGGTCA ACCCGGCCTG 
CCCGGATTCC CAGGAACACC AGGGCTTCCT GGACCAAAGG GTATGAATGG TCCTCCTGGT 
AACCCTGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGGCGTCC TGGACCACCA 
GGACCTCCTG GTGAAAAAGG CAAGCCAGGT CAAGATGGCA TTCCTGGACC AGCAGGACAA 
AAGGGAGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CTCCTGGACT CCCAGGACTT 
TCTGGTCAAA AGGGTGATGG AGGAGCCCCT GGCCTTCCAG GAAATCCTGG CCTTCCAGGT 
TCAAAGGGTG AACCAGGCTT TCAGGGATTC CCTGGTATGC CAGGTCCCCC AGGTCCCCCT 
GGTTCTCCTG GCCCATCTTT GGAAGGTCCC AAAGGAAACC CTGGGCCTCA AGGCCCTCCT 
GGGAGACCAG GTTTTCAAGG TCCACCTGGT ACAGAAGGTC CTCGTGGCCC CCCTGGAAAT 
GGAGGTATTA AAGGAGAGAG GGGAAATCCA GGTCCACCTG GGCAACCAGG CTTACCTGGC 
TTGAAAGGAG ATCAAGGACC ACCAGGCCTT CCGGGTAACC CTGGCCGACC AGGTCTTAAT 
GGAATGAAAG GAGATCCTGG TCTCCCTGGT GTTCCAGGAT TCCCAGGAAT GAAAGGACCC 
ATTGGAGTAC CAGGCTCAAC TGGCCCTGAT GGGGAACCAG GTCTTACGGG GCCCCCAGGT 
CCTCCTGGGT TGCCTGGTCC TTCAGGACAG AGTATTGTAA TCAAAGGAGA TCCTGGACCT 
CCAGGAATTC CTGGACAACC TGGATTAAAA GGTCTACCAG GACTTCCAGG CCCTCAAGGT 
CTACCAGGTC CAATTGGCCC TCCAGGAGAT CCAGGACGCA ATGGACTCCC AGGCTTTGAT 
GGTGCAGGAG GGCGCAAAGG AGACCCAGGC CTGCCGGGGC AGCCAGGTAG CCGTGGATTG 
GATGGTCCCC CAGGACCAGA TGGATTGCAA GGACCTCCAG GGCCCCCGGG AACCACCTCT 
GTTGCCCATG GATTCCTCAT CACACGTCAC AGTCAGACAA CAGATGCACC ACAATGCCCA 
CAGGGAACAG TCCATATCTA TGATGGCTTT TCTCTCTTAT ATGTACAAGG AAATAAAAGA 
GCCCATGGTC AAGACTTGGG GACAGCTGGC AGCTGCCTTC GTCGCTTCAG TACCATGCCT 
TTCATGTTCT GCAACATTAA CAACGTTTGT AACTTTGCTT CAAGAAATGA CTATTCTTAC 
TGGCTTTCCA CCCCAGAGCC CATGCCAATG AACATGGAAC CCCTGAAGGG CCAGAGCATC 
CAGCCATTCA TTAGTCGATG TGCAGTATGT GAGGCACCAG CTGTGGTGAT TGCAGTTCAC 
AGCCAGACTA TTCAGATTCC TCACTGTCCT CAGGGGTGGG ACTCTCTGTG GATTGGCTAT 
TCATTCATGA TGCATACAAG TGCAGGAGCA GAAGGCTCTG GCCAAGCCCT AGCCTCTCCT 
GGCTCCTGTT TGGAAGAATT TCGTTCAGCT CCCTTCATCG AATGTCATGG ACGAGGGACA 
TGCAATTACT ATGCCAATTC TTACAGCTTT TGGCTGGCCA CTGTTGATAT GTCAGACATG 
TTCAGTAAAC CTCAGTCAGA GACACTGAAA GCAGGCGACT TGAGGACACG TATTAGCCGA 
TGTCAAGTAT GCATGAAAAG GACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008756756.1
CDS	400..5466
Translation	MQVPGVCLAAGWFLLALCLWGQPGEAAACHGCSSGSKCDCSGIKGEKGERGFPGLEGHPGLPGFPGPEGPPGPRGQKGDDGIPGPPGPKGIRGPPGLPGFPGTPGLPGMPGHDGAPGPQGIPGCNGTKGERGFPGSPGFPGLQGPPGPPGIPGMKGEPGSIIMSSLPGPKGNQGYPGPPGIQGPPGPKGVSGPSGPPGPPGLMGPPGPPGPPGPKGNMGLNFQGPKGEKGEQGLQGPPGPPGQISEQKRPIDLDFQKGDQGVPGDRGPPGPPGIPGPPGPPGGVKGEKGEPGEPGKRGKPGKDGETGQPGIPGLPGDPGYPGEPGRHGEKGQKGDTGPPGPPGFVIPTPGTGVTIGAKGNIGQPGLPGEKGERGFPGIQGPPGFPGPPGTAVMGPPGPPGYPGERGQKGDEGPPGICIPGSPGLDGQPGAPGFPGPPGPPGPQLPPSDEICKAGPPGPPGSPGDQGLQGEQGVKGDKGDTCFNCIGTGISGPPGQPGLPGLPGPPGSLGIPGEKGEKGQAGTTGPKGLPGIPGPPGAPGFPGSKGDPGDVLTLPGMKGEKGELGSPGAPGLPGLPGSPGKDGLPGLPGPKGEPGGITFKGERGPPGNPGLPGLPGNMGPTGPVGFGPPGPVGEKGIQGVAGNPGQPGLPGPKGDPGQTISQPGKPGLPGNPGRDGEVGLPGDPGLPGQPGLPGIPGSKGEPGIPGIGPPGPPGPKGFPGIPGPPGPPGAPGRMGPEGPPGLPGFPGPKGEPGFGLPGPPGPPGFPGFKGTPGPKGDRGLPGPPGPPGHTGLDGLPGPKGDAGPNGQPGPIGPPGLPGIGLQGPPGPPGTPGPIGQPGLHGIPGEKGDPGPPGLDVPGLPGERGSPGLPGAPGLIGPPGSPGIPGRAGVPGFPGVKGEMGMMGPPGPPGPLGTPGRSGAPGLKGDDGMQGQPGLPGPVGEKGGKGEPGLPGPPGPMRPDFLGSKGEKGDPGLPGIPGLSGPKGYQGLPGDPGQPGLSGQPGSPGLPGPKGTPGLPGPPGLTGPPGPKGNIGDMGFPGPQGVDGPPGPPGGPGQPGSPGLPGQKGSKGEPGVSGIGLPGLPGPKGEPGLPGYPGNPGIKGSMGEAGLPGLPGSPGAKGQPGLPGFPGTPGLPGPKGMNGPPGNPGLPGEPGPVGGGGRPGPPGPPGEKGKPGQDGIPGPAGQKGEPGQPGFGIPGPPGLPGLSGQKGDGGAPGLPGNPGLPGSKGEPGFQGFPGMPGPPGPPGSPGPSLEGPKGNPGPQGPPGRPGFQGPPGTEGPRGPPGNGGIKGERGNPGPPGQPGLPGLKGDQGPPGLPGNPGRPGLNGMKGDPGLPGVPGFPGMKGPIGVPGSTGPDGEPGLTGPPGPPGLPGPSGQSIVIKGDPGPPGIPGQPGLKGLPGLPGPQGLPGPIGPPGDPGRNGLPGFDGAGGRKGDPGLPGQPGSRGLDGPPGPDGLQGPPGPPGTTSVAHGFLITRHSQTTDAPQCPQGTVHIYDGFSLLYVQGNKRAHGQDLGTAGSCLRRFSTMPFMFCNINNVCNFASRNDYSYWLSTPEPMPMNMEPLKGQSIQPFISRCAVCEAPAVVIAVHSQTIQIPHCPQGWDSLWIGYSFMMHTSAGAEGSGQALASPGSCLEEFRSAPFIECHGRGTCNYYANSYSFWLATVDMSDMFSKPQSETLKAGDLRTRISRCQVCMKRT

Target ORF information:

RefSeq Version	XM_008758534.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus collagen type IV alpha 5 chain (Col4a5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008758534.1

ORF Insert Sequence:

ATGCAAGTGC CTGGAGTGTG CCTGGCCGCC GGCTGGTTCT TACTGGCCCT GTGTCTGTGG 
GGGCAGCCGG GAGAGGCTGC GGCCTGCCAT GGGTGTTCTT CTGGATCAAA ATGTGACTGC 
AGTGGTATAA AAGGAGAAAA GGGAGAACGG GGCTTTCCAG GCTTAGAAGG CCATCCAGGT 
TTACCAGGAT TTCCAGGTCC AGAAGGGCCT CCAGGACCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGA ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGCCTTCC TGGGATGCCA GGCCACGATG GGGCTCCAGG ACCTCAAGGT 
ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGCGGATTTC CAGGCAGTCC AGGCTTTCCC 
GGTTTACAAG GACCTCCAGG ACCACCTGGG ATCCCAGGTA TGAAGGGGGA GCCAGGCAGT 
ATAATCATGT CATCTCTGCC AGGGCCAAAG GGTAATCAAG GATACCCAGG TCCTCCTGGA 
ATACAAGGTC CACCTGGTCC CAAGGGTGTA TCAGGGCCAA GTGGTCCTCC AGGACCACCA 
GGTTTAATGG GCCCTCCTGG CCCACCAGGC CCACCGGGAC CAAAGGGGAA TATGGGCTTA 
AATTTCCAGG GACCCAAAGG CGAAAAAGGT GAACAAGGTC TTCAGGGTCC ACCTGGTCCA 
CCTGGGCAGA TCAGTGAACA GAAAAGACCA ATTGACCTAG ATTTTCAGAA AGGCGATCAG 
GGAGTTCCCG GTGATAGAGG GCCTCCTGGA CCTCCGGGAA TACCTGGTCC TCCCGGTCCT 
CCTGGTGGTG TGAAAGGTGA AAAGGGTGAG CCCGGAGAGC CAGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAGACTGG TCAACCAGGA ATCCCAGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGTGAAC CAGGAAGGCA TGGTGAAAAG GGCCAAAAGG GTGATACTGG CCCACCTGGA 
CCACCTGGAT TTGTAATTCC TACACCTGGA ACTGGTGTGA CTATTGGAGC AAAAGGAAAT 
ATTGGGCAAC CTGGTTTGCC TGGAGAAAAA GGAGAGCGAG GATTTCCTGG CATACAAGGT 
CCACCTGGCT TTCCTGGACC TCCAGGGACA GCTGTCATGG GTCCCCCTGG TCCTCCTGGA 
TATCCTGGTG AAAGGGGCCA GAAAGGTGAT GAAGGTCCCC CTGGAATTTG TATTCCTGGA 
TCTCCTGGAC TTGATGGACA GCCTGGAGCT CCTGGCTTTC CAGGACCTCC TGGTCCTCCT 
GGCCCCCAGT TACCCCCGAG TGATGAAATC TGTAAAGCAG GCCCTCCAGG GCCCCCAGGA 
TCTCCAGGTG ACCAAGGACT CCAAGGAGAG CAAGGAGTAA AAGGTGACAA AGGTGATACT 
TGCTTCAACT GTATTGGAAC TGGCATTTCA GGGCCTCCAG GCCAACCAGG TTTACCAGGT 
CTCCCAGGTC CTCCAGGATC TCTTGGAATC CCTGGAGAGA AGGGCGAGAA AGGACAAGCT 
GGGACAACTG GTCCAAAAGG ATTGCCAGGC ATTCCTGGAC CTCCAGGTGC TCCAGGCTTT 
CCAGGGTCTA AGGGTGACCC TGGTGATGTT CTCACTCTTC CGGGAATGAA AGGTGAAAAA 
GGAGAATTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTC TACCTGGTAG TCCTGGAAAG 
GATGGTTTGC CAGGACTTCC TGGCCCCAAA GGAGAGCCCG GTGGAATCAC TTTTAAGGGT 
GAAAGGGGTC CTCCTGGGAA CCCAGGTTTA CCAGGTCTCC CAGGAAATAT GGGACCTACA 
GGCCCTGTTG GTTTTGGGCC TCCAGGCCCC GTAGGTGAAA AAGGCATACA AGGTGTGGCG 
GGAAATCCAG GCCAGCCAGG ATTACCAGGT CCTAAAGGTG ATCCAGGCCA GACCATCAGC 
CAGCCAGGAA AGCCTGGCTT GCCAGGGAAT CCAGGCAGAG ATGGTGAAGT GGGTCTTCCA 
GGTGATCCTG GACTTCCAGG CCAACCAGGC CTACCAGGAA TACCTGGTAG CAAAGGAGAA 
CCAGGTATCC CTGGGATTGG GCCTCCAGGA CCACCTGGTC CCAAAGGTTT TCCAGGAATT 
CCTGGACCTC CAGGACCTCC TGGGGCCCCT GGAAGAATGG GCCCAGAAGG GCCTCCTGGG 
TTACCGGGCT TTCCAGGACC CAAGGGAGAG CCAGGATTTG GGTTACCTGG GCCACCTGGG 
CCACCAGGAT TCCCAGGTTT CAAAGGAACA CCTGGTCCAA AAGGTGATCG TGGTTTACCA 
GGACCTCCAG GTCCTCCAGG ACACACTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAT 
GCTGGACCAA ATGGACAACC TGGCCCAATA GGGCCACCAG GGCTGCCAGG AATTGGTCTT 
CAGGGACCAC CAGGACCACC AGGGACTCCA GGGCCAATAG GCCAACCTGG CTTACATGGA 
ATACCAGGAG AGAAGGGAGA TCCAGGACCT CCTGGGCTTG ATGTTCCAGG ACTCCCTGGA 
GAGAGAGGTA GTCCAGGGCT CCCTGGAGCC CCTGGTCTTA TAGGACCTCC AGGATCACCT 
GGTATCCCTG GAAGAGCAGG TGTCCCTGGA TTTCCAGGTG TCAAAGGCGA AATGGGTATG 
ATGGGACCTC CAGGCCCACC GGGACCTTTG GGTACTCCTG GCAGGAGTGG TGCTCCTGGT 
CTTAAAGGTG ATGACGGCAT GCAAGGTCAG CCAGGTCTCC CTGGCCCTGT AGGAGAAAAG 
GGTGGTAAGG GAGAACCTGG CCTTCCAGGT CCTCCTGGAC CAATGCGTCC AGATTTCCTG 
GGATCAAAAG GAGAAAAGGG TGATCCTGGC TTACCAGGTA TTCCTGGACT TTCCGGACCA 
AAAGGCTACC AGGGATTACC TGGAGACCCA GGGCAACCTG GGCTGAGTGG ACAGCCGGGA 
TCACCAGGAC TACCAGGTCC CAAAGGTACT CCTGGTCTCC CTGGTCCACC AGGACTTACC 
GGACCTCCTG GACCTAAAGG AAACATTGGC GACATGGGTT TTCCTGGCCC TCAAGGTGTA 
GATGGGCCTC CTGGACCTCC TGGAGGTCCT GGACAACCTG GCTCTCCCGG GTTGCCTGGC 
CAGAAAGGAA GTAAAGGAGA ACCTGGAGTT TCAGGCATTG GTCTCCCTGG TCTTCCTGGT 
CCAAAGGGTG AACCTGGTCT GCCTGGATAT CCAGGAAACC CAGGTATCAA AGGTTCTATG 
GGCGAGGCTG GTCTGCCTGG CTTACCAGGA AGCCCTGGAG CAAAAGGTCA ACCCGGCCTG 
CCCGGATTCC CAGGAACACC AGGGCTTCCT GGACCAAAGG GTATGAATGG TCCTCCTGGT 
AACCCTGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGGCGTCC TGGACCACCA 
GGACCTCCTG GTGAAAAAGG CAAGCCAGGT CAAGATGGCA TTCCTGGACC AGCAGGACAA 
AAGGGAGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CTCCTGGACT CCCAGGACTT 
TCTGGTCAAA AGGGTGATGG AGGAGCCCCT GGCCTTCCAG GAAATCCTGG CCTTCCAGGT 
TCAAAGGGTG AACCAGGCTT TCAGGGATTC CCTGGTATGC CAGGTCCCCC AGGTCCCCCT 
GGTTCTCCTG GCCCATCTTT GGAAGGTCCC AAAGGAAACC CTGGGCCTCA AGGCCCTCCT 
GGGAGACCAG GTTTTCAAGG TCCACCTGGT ACAGAAGGTC CTCGTGGCCC CCCTGGAAAT 
GGAGGTATTA AAGGAGAGAG GGGAAATCCA GGTCCACCTG GGCAACCAGG CTTACCTGGC 
TTGAAAGGAG ATCAAGGACC ACCAGGCCTT CCGGGTAACC CTGGCCGACC AGGTCTTAAT 
GGAATGAAAG GAGATCCTGG TCTCCCTGGT GTTCCAGGAT TCCCAGGAAT GAAAGGACCC 
ATTGGAGTAC CAGGCTCAAC TGGCCCTGAT GGGGAACCAG GTCTTACGGG GCCCCCAGGT 
CCTCCTGGGT TGCCTGGTCC TTCAGGACAG AGTATTGTAA TCAAAGGAGA TCCTGGACCT 
CCAGGAATTC CTGGACAACC TGGATTAAAA GGTCTACCAG GACTTCCAGG CCCTCAAGGT 
CTACCAGGTC CAATTGGCCC TCCAGGAGAT CCAGGACGCA ATGGACTCCC AGGCTTTGAT 
GGTGCAGGAG GGCGCAAAGG AGACCCAGGC CTGCCGGGGC AGCCAGGTAG CCGTGGATTG 
GATGGTCCCC CAGGACCAGA TGGATTGCAA GGACCTCCAG GGCCCCCGGG AACCACCTCT 
GTTGCCCATG GATTCCTCAT CACACGTCAC AGTCAGACAA CAGATGCACC ACAATGCCCA 
CAGGGAACAG TCCATATCTA TGATGGCTTT TCTCTCTTAT ATGTACAAGG AAATAAAAGA 
GCCCATGGTC AAGACTTGGG GACAGCTGGC AGCTGCCTTC GTCGCTTCAG TACCATGCCT 
TTCATGTTCT GCAACATTAA CAACGTTTGT AACTTTGCTT CAAGAAATGA CTATTCTTAC 
TGGCTTTCCA CCCCAGAGCC CATGCCAATG AACATGGAAC CCCTGAAGGG CCAGAGCATC 
CAGCCATTCA TTAGTCGATG TGCAGTATGT GAGGCACCAG CTGTGGTGAT TGCAGTTCAC 
AGCCAGACTA TTCAGATTCC TCACTGTCCT CAGGGGTGGG ACTCTCTGTG GATTGGCTAT 
TCATTCATGA TGCATACAAG TGCAGGAGCA GAAGGCTCTG GCCAAGCCCT AGCCTCTCCT 
GGCTCCTGTT TGGAAGAATT TCGTTCAGCT CCCTTCATCG AATGTCATGG ACGAGGGACA 
TGCAATTACT ATGCCAATTC TTACAGCTTT TGGCTGGCCA CTGTTGATAT GTCAGACATG 
TTCAGTAAAC CTCAGTCAGA GACACTGAAA GCAGGCGACT TGAGGACACG TATTAGCCGA 
TGTCAAGTAT GCATGAAAAG GACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Col4a5 ( XM_008773475.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008771697.1 Rattus norvegicus collagen type IV alpha 5 chain (Col4a5), transcript variant X3, mRNA.

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CloneID	ORa48575
Clone ID Related Accession (Same CDS sequence)	XM_008758534.1 , XM_008773475.1
Accession Version	XM_008773475.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5067bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	collagen alpha-5(IV) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAGTGC CTGGAGTGTG CCTGGCCGCC GGCTGGTTCT TACTGGCCCT GTGTCTGTGG 
GGGCAGCCGG GAGAGGCTGC GGCCTGCCAT GGGTGTTCTT CTGGATCAAA ATGTGACTGC 
AGTGGTATAA AAGGAGAAAA GGGAGAACGG GGCTTTCCAG GCTTAGAAGG CCATCCAGGT 
TTACCAGGAT TTCCAGGTCC AGAAGGGCCT CCAGGACCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGA ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGCCTTCC TGGGATGCCA GGCCACGATG GGGCTCCAGG ACCTCAAGGT 
ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGCGGATTTC CAGGCAGTCC AGGCTTTCCC 
GGTTTACAAG GACCTCCAGG ACCACCTGGG ATCCCAGGTA TGAAGGGGGA GCCAGGCAGT 
ATAATCATGT CATCTCTGCC AGGGCCAAAG GGTAATCAAG GATACCCAGG TCCTCCTGGA 
ATACAAGGTC CACCTGGTCC CAAGGGTGTA TCAGGGCCAA GTGGTCCTCC AGGACCACCA 
GGTTTAATGG GCCCTCCTGG CCCACCAGGC CCACCGGGAC CAAAGGGGAA TATGGGCTTA 
AATTTCCAGG GACCCAAAGG CGAAAAAGGT GAACAAGGTC TTCAGGGTCC ACCTGGTCCA 
CCTGGGCAGA TCAGTGAACA GAAAAGACCA ATTGACCTAG ATTTTCAGAA AGGCGATCAG 
GGAGTTCCCG GTGATAGAGG GCCTCCTGGA CCTCCGGGAA TACCTGGTCC TCCCGGTCCT 
CCTGGTGGTG TGAAAGGTGA AAAGGGTGAG CCCGGAGAGC CAGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAGACTGG TCAACCAGGA ATCCCAGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGTGAAC CAGGAAGGCA TGGTGAAAAG GGCCAAAAGG GTGATACTGG CCCACCTGGA 
CCACCTGGAT TTGTAATTCC TACACCTGGA ACTGGTGTGA CTATTGGAGC AAAAGGAAAT 
ATTGGGCAAC CTGGTTTGCC TGGAGAAAAA GGAGAGCGAG GATTTCCTGG CATACAAGGT 
CCACCTGGCT TTCCTGGACC TCCAGGGACA GCTGTCATGG GTCCCCCTGG TCCTCCTGGA 
TATCCTGGTG AAAGGGGCCA GAAAGGTGAT GAAGGTCCCC CTGGAATTTG TATTCCTGGA 
TCTCCTGGAC TTGATGGACA GCCTGGAGCT CCTGGCTTTC CAGGACCTCC TGGTCCTCCT 
GGCCCCCAGT TACCCCCGAG TGATGAAATC TGTAAAGCAG GCCCTCCAGG GCCCCCAGGA 
TCTCCAGGTG ACCAAGGACT CCAAGGAGAG CAAGGAGTAA AAGGTGACAA AGGTGATACT 
TGCTTCAACT GTATTGGAAC TGGCATTTCA GGGCCTCCAG GCCAACCAGG TTTACCAGGT 
CTCCCAGGTC CTCCAGGATC TCTTGGAATC CCTGGAGAGA AGGGCGAGAA AGGACAAGCT 
GGGACAACTG GTCCAAAAGG ATTGCCAGGC ATTCCTGGAC CTCCAGGTGC TCCAGGCTTT 
CCAGGGTCTA AGGGTGACCC TGGTGATGTT CTCACTCTTC CGGGAATGAA AGGTGAAAAA 
GGAGAATTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTC TACCTGGTAG TCCTGGAAAG 
GATGGTTTGC CAGGACTTCC TGGCCCCAAA GGAGAGCCCG GTGGAATCAC TTTTAAGGGT 
GAAAGGGGTC CTCCTGGGAA CCCAGGTTTA CCAGGTCTCC CAGGAAATAT GGGACCTACA 
GGCCCTGTTG GTTTTGGGCC TCCAGGCCCC GTAGGTGAAA AAGGCATACA AGGTGTGGCG 
GGAAATCCAG GCCAGCCAGG ATTACCAGGT CCTAAAGGTG ATCCAGGCCA GACCATCAGC 
CAGCCAGGAA AGCCTGGCTT GCCAGGGAAT CCAGGCAGAG ATGGTGAAGT GGGTCTTCCA 
GGTGATCCTG GACTTCCAGG CCAACCAGGC CTACCAGGAA TACCTGGTAG CAAAGGAGAA 
CCAGGTATCC CTGGGATTGG GCCTCCAGGA CCACCTGGTC CCAAAGGTTT TCCAGGAATT 
CCTGGACCTC CAGGACCTCC TGGGGCCCCT GGAAGAATGG GCCCAGAAGG GCCTCCTGGG 
TTACCGGGCT TTCCAGGACC CAAGGGAGAG CCAGGATTTG GGTTACCTGG GCCACCTGGG 
CCACCAGGAT TCCCAGGTTT CAAAGGAACA CCTGGTCCAA AAGGTGATCG TGGTTTACCA 
GGACCTCCAG GTCCTCCAGG ACACACTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAT 
GCTGGACCAA ATGGACAACC TGGCCCAATA GGGCCACCAG GGCTGCCAGG AATTGGTCTT 
CAGGGACCAC CAGGACCACC AGGGACTCCA GGGCCAATAG GCCAACCTGG CTTACATGGA 
ATACCAGGAG AGAAGGGAGA TCCAGGACCT CCTGGGCTTG ATGTTCCAGG ACTCCCTGGA 
GAGAGAGGTA GTCCAGGGCT CCCTGGAGCC CCTGGTCTTA TAGGACCTCC AGGATCACCT 
GGTATCCCTG GAAGAGCAGG TGTCCCTGGA TTTCCAGGTG TCAAAGGCGA AATGGGTATG 
ATGGGACCTC CAGGCCCACC GGGACCTTTG GGTACTCCTG GCAGGAGTGG TGCTCCTGGT 
CTTAAAGGTG ATGACGGCAT GCAAGGTCAG CCAGGTCTCC CTGGCCCTGT AGGAGAAAAG 
GGTGGTAAGG GAGAACCTGG CCTTCCAGGT CCTCCTGGAC CAATGCGTCC AGATTTCCTG 
GGATCAAAAG GAGAAAAGGG TGATCCTGGC TTACCAGGTA TTCCTGGACT TTCCGGACCA 
AAAGGCTACC AGGGATTACC TGGAGACCCA GGGCAACCTG GGCTGAGTGG ACAGCCGGGA 
TCACCAGGAC TACCAGGTCC CAAAGGTACT CCTGGTCTCC CTGGTCCACC AGGACTTACC 
GGACCTCCTG GACCTAAAGG AAACATTGGC GACATGGGTT TTCCTGGCCC TCAAGGTGTA 
GATGGGCCTC CTGGACCTCC TGGAGGTCCT GGACAACCTG GCTCTCCCGG GTTGCCTGGC 
CAGAAAGGAA GTAAAGGAGA ACCTGGAGTT TCAGGCATTG GTCTCCCTGG TCTTCCTGGT 
CCAAAGGGTG AACCTGGTCT GCCTGGATAT CCAGGAAACC CAGGTATCAA AGGTTCTATG 
GGCGAGGCTG GTCTGCCTGG CTTACCAGGA AGCCCTGGAG CAAAAGGTCA ACCCGGCCTG 
CCCGGATTCC CAGGAACACC AGGGCTTCCT GGACCAAAGG GTATGAATGG TCCTCCTGGT 
AACCCTGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGGCGTCC TGGACCACCA 
GGACCTCCTG GTGAAAAAGG CAAGCCAGGT CAAGATGGCA TTCCTGGACC AGCAGGACAA 
AAGGGAGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CTCCTGGACT CCCAGGACTT 
TCTGGTCAAA AGGGTGATGG AGGAGCCCCT GGCCTTCCAG GAAATCCTGG CCTTCCAGGT 
TCAAAGGGTG AACCAGGCTT TCAGGGATTC CCTGGTATGC CAGGTCCCCC AGGTCCCCCT 
GGTTCTCCTG GCCCATCTTT GGAAGGTCCC AAAGGAAACC CTGGGCCTCA AGGCCCTCCT 
GGGAGACCAG GTTTTCAAGG TCCACCTGGT ACAGAAGGTC CTCGTGGCCC CCCTGGAAAT 
GGAGGTATTA AAGGAGAGAG GGGAAATCCA GGTCCACCTG GGCAACCAGG CTTACCTGGC 
TTGAAAGGAG ATCAAGGACC ACCAGGCCTT CCGGGTAACC CTGGCCGACC AGGTCTTAAT 
GGAATGAAAG GAGATCCTGG TCTCCCTGGT GTTCCAGGAT TCCCAGGAAT GAAAGGACCC 
ATTGGAGTAC CAGGCTCAAC TGGCCCTGAT GGGGAACCAG GTCTTACGGG GCCCCCAGGT 
CCTCCTGGGT TGCCTGGTCC TTCAGGACAG AGTATTGTAA TCAAAGGAGA TCCTGGACCT 
CCAGGAATTC CTGGACAACC TGGATTAAAA GGTCTACCAG GACTTCCAGG CCCTCAAGGT 
CTACCAGGTC CAATTGGCCC TCCAGGAGAT CCAGGACGCA ATGGACTCCC AGGCTTTGAT 
GGTGCAGGAG GGCGCAAAGG AGACCCAGGC CTGCCGGGGC AGCCAGGTAG CCGTGGATTG 
GATGGTCCCC CAGGACCAGA TGGATTGCAA GGACCTCCAG GGCCCCCGGG AACCACCTCT 
GTTGCCCATG GATTCCTCAT CACACGTCAC AGTCAGACAA CAGATGCACC ACAATGCCCA 
CAGGGAACAG TCCATATCTA TGATGGCTTT TCTCTCTTAT ATGTACAAGG AAATAAAAGA 
GCCCATGGTC AAGACTTGGG GACAGCTGGC AGCTGCCTTC GTCGCTTCAG TACCATGCCT 
TTCATGTTCT GCAACATTAA CAACGTTTGT AACTTTGCTT CAAGAAATGA CTATTCTTAC 
TGGCTTTCCA CCCCAGAGCC CATGCCAATG AACATGGAAC CCCTGAAGGG CCAGAGCATC 
CAGCCATTCA TTAGTCGATG TGCAGTATGT GAGGCACCAG CTGTGGTGAT TGCAGTTCAC 
AGCCAGACTA TTCAGATTCC TCACTGTCCT CAGGGGTGGG ACTCTCTGTG GATTGGCTAT 
TCATTCATGA TGCATACAAG TGCAGGAGCA GAAGGCTCTG GCCAAGCCCT AGCCTCTCCT 
GGCTCCTGTT TGGAAGAATT TCGTTCAGCT CCCTTCATCG AATGTCATGG ACGAGGGACA 
TGCAATTACT ATGCCAATTC TTACAGCTTT TGGCTGGCCA CTGTTGATAT GTCAGACATG 
TTCAGTAAAC CTCAGTCAGA GACACTGAAA GCAGGCGACT TGAGGACACG TATTAGCCGA 
TGTCAAGTAT GCATGAAAAG GACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008771697.1
CDS	400..5466
Translation	MQVPGVCLAAGWFLLALCLWGQPGEAAACHGCSSGSKCDCSGIKGEKGERGFPGLEGHPGLPGFPGPEGPPGPRGQKGDDGIPGPPGPKGIRGPPGLPGFPGTPGLPGMPGHDGAPGPQGIPGCNGTKGERGFPGSPGFPGLQGPPGPPGIPGMKGEPGSIIMSSLPGPKGNQGYPGPPGIQGPPGPKGVSGPSGPPGPPGLMGPPGPPGPPGPKGNMGLNFQGPKGEKGEQGLQGPPGPPGQISEQKRPIDLDFQKGDQGVPGDRGPPGPPGIPGPPGPPGGVKGEKGEPGEPGKRGKPGKDGETGQPGIPGLPGDPGYPGEPGRHGEKGQKGDTGPPGPPGFVIPTPGTGVTIGAKGNIGQPGLPGEKGERGFPGIQGPPGFPGPPGTAVMGPPGPPGYPGERGQKGDEGPPGICIPGSPGLDGQPGAPGFPGPPGPPGPQLPPSDEICKAGPPGPPGSPGDQGLQGEQGVKGDKGDTCFNCIGTGISGPPGQPGLPGLPGPPGSLGIPGEKGEKGQAGTTGPKGLPGIPGPPGAPGFPGSKGDPGDVLTLPGMKGEKGELGSPGAPGLPGLPGSPGKDGLPGLPGPKGEPGGITFKGERGPPGNPGLPGLPGNMGPTGPVGFGPPGPVGEKGIQGVAGNPGQPGLPGPKGDPGQTISQPGKPGLPGNPGRDGEVGLPGDPGLPGQPGLPGIPGSKGEPGIPGIGPPGPPGPKGFPGIPGPPGPPGAPGRMGPEGPPGLPGFPGPKGEPGFGLPGPPGPPGFPGFKGTPGPKGDRGLPGPPGPPGHTGLDGLPGPKGDAGPNGQPGPIGPPGLPGIGLQGPPGPPGTPGPIGQPGLHGIPGEKGDPGPPGLDVPGLPGERGSPGLPGAPGLIGPPGSPGIPGRAGVPGFPGVKGEMGMMGPPGPPGPLGTPGRSGAPGLKGDDGMQGQPGLPGPVGEKGGKGEPGLPGPPGPMRPDFLGSKGEKGDPGLPGIPGLSGPKGYQGLPGDPGQPGLSGQPGSPGLPGPKGTPGLPGPPGLTGPPGPKGNIGDMGFPGPQGVDGPPGPPGGPGQPGSPGLPGQKGSKGEPGVSGIGLPGLPGPKGEPGLPGYPGNPGIKGSMGEAGLPGLPGSPGAKGQPGLPGFPGTPGLPGPKGMNGPPGNPGLPGEPGPVGGGGRPGPPGPPGEKGKPGQDGIPGPAGQKGEPGQPGFGIPGPPGLPGLSGQKGDGGAPGLPGNPGLPGSKGEPGFQGFPGMPGPPGPPGSPGPSLEGPKGNPGPQGPPGRPGFQGPPGTEGPRGPPGNGGIKGERGNPGPPGQPGLPGLKGDQGPPGLPGNPGRPGLNGMKGDPGLPGVPGFPGMKGPIGVPGSTGPDGEPGLTGPPGPPGLPGPSGQSIVIKGDPGPPGIPGQPGLKGLPGLPGPQGLPGPIGPPGDPGRNGLPGFDGAGGRKGDPGLPGQPGSRGLDGPPGPDGLQGPPGPPGTTSVAHGFLITRHSQTTDAPQCPQGTVHIYDGFSLLYVQGNKRAHGQDLGTAGSCLRRFSTMPFMFCNINNVCNFASRNDYSYWLSTPEPMPMNMEPLKGQSIQPFISRCAVCEAPAVVIAVHSQTIQIPHCPQGWDSLWIGYSFMMHTSAGAEGSGQALASPGSCLEEFRSAPFIECHGRGTCNYYANSYSFWLATVDMSDMFSKPQSETLKAGDLRTRISRCQVCMKRT

Target ORF information:

RefSeq Version	XM_008773475.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus collagen type IV alpha 5 chain (Col4a5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008773475.1

ORF Insert Sequence:

ATGCAAGTGC CTGGAGTGTG CCTGGCCGCC GGCTGGTTCT TACTGGCCCT GTGTCTGTGG 
GGGCAGCCGG GAGAGGCTGC GGCCTGCCAT GGGTGTTCTT CTGGATCAAA ATGTGACTGC 
AGTGGTATAA AAGGAGAAAA GGGAGAACGG GGCTTTCCAG GCTTAGAAGG CCATCCAGGT 
TTACCAGGAT TTCCAGGTCC AGAAGGGCCT CCAGGACCTC GGGGACAAAA GGGTGATGAT 
GGAATTCCAG GGCCACCAGG ACCAAAAGGA ATCAGAGGTC CTCCTGGACT TCCTGGATTT 
CCAGGGACAC CAGGCCTTCC TGGGATGCCA GGCCACGATG GGGCTCCAGG ACCTCAAGGT 
ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGCGGATTTC CAGGCAGTCC AGGCTTTCCC 
GGTTTACAAG GACCTCCAGG ACCACCTGGG ATCCCAGGTA TGAAGGGGGA GCCAGGCAGT 
ATAATCATGT CATCTCTGCC AGGGCCAAAG GGTAATCAAG GATACCCAGG TCCTCCTGGA 
ATACAAGGTC CACCTGGTCC CAAGGGTGTA TCAGGGCCAA GTGGTCCTCC AGGACCACCA 
GGTTTAATGG GCCCTCCTGG CCCACCAGGC CCACCGGGAC CAAAGGGGAA TATGGGCTTA 
AATTTCCAGG GACCCAAAGG CGAAAAAGGT GAACAAGGTC TTCAGGGTCC ACCTGGTCCA 
CCTGGGCAGA TCAGTGAACA GAAAAGACCA ATTGACCTAG ATTTTCAGAA AGGCGATCAG 
GGAGTTCCCG GTGATAGAGG GCCTCCTGGA CCTCCGGGAA TACCTGGTCC TCCCGGTCCT 
CCTGGTGGTG TGAAAGGTGA AAAGGGTGAG CCCGGAGAGC CAGGCAAAAG AGGTAAACCA 
GGCAAAGATG GAGAGACTGG TCAACCAGGA ATCCCAGGTT TGCCTGGTGA TCCTGGTTAC 
CCTGGTGAAC CAGGAAGGCA TGGTGAAAAG GGCCAAAAGG GTGATACTGG CCCACCTGGA 
CCACCTGGAT TTGTAATTCC TACACCTGGA ACTGGTGTGA CTATTGGAGC AAAAGGAAAT 
ATTGGGCAAC CTGGTTTGCC TGGAGAAAAA GGAGAGCGAG GATTTCCTGG CATACAAGGT 
CCACCTGGCT TTCCTGGACC TCCAGGGACA GCTGTCATGG GTCCCCCTGG TCCTCCTGGA 
TATCCTGGTG AAAGGGGCCA GAAAGGTGAT GAAGGTCCCC CTGGAATTTG TATTCCTGGA 
TCTCCTGGAC TTGATGGACA GCCTGGAGCT CCTGGCTTTC CAGGACCTCC TGGTCCTCCT 
GGCCCCCAGT TACCCCCGAG TGATGAAATC TGTAAAGCAG GCCCTCCAGG GCCCCCAGGA 
TCTCCAGGTG ACCAAGGACT CCAAGGAGAG CAAGGAGTAA AAGGTGACAA AGGTGATACT 
TGCTTCAACT GTATTGGAAC TGGCATTTCA GGGCCTCCAG GCCAACCAGG TTTACCAGGT 
CTCCCAGGTC CTCCAGGATC TCTTGGAATC CCTGGAGAGA AGGGCGAGAA AGGACAAGCT 
GGGACAACTG GTCCAAAAGG ATTGCCAGGC ATTCCTGGAC CTCCAGGTGC TCCAGGCTTT 
CCAGGGTCTA AGGGTGACCC TGGTGATGTT CTCACTCTTC CGGGAATGAA AGGTGAAAAA 
GGAGAATTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTC TACCTGGTAG TCCTGGAAAG 
GATGGTTTGC CAGGACTTCC TGGCCCCAAA GGAGAGCCCG GTGGAATCAC TTTTAAGGGT 
GAAAGGGGTC CTCCTGGGAA CCCAGGTTTA CCAGGTCTCC CAGGAAATAT GGGACCTACA 
GGCCCTGTTG GTTTTGGGCC TCCAGGCCCC GTAGGTGAAA AAGGCATACA AGGTGTGGCG 
GGAAATCCAG GCCAGCCAGG ATTACCAGGT CCTAAAGGTG ATCCAGGCCA GACCATCAGC 
CAGCCAGGAA AGCCTGGCTT GCCAGGGAAT CCAGGCAGAG ATGGTGAAGT GGGTCTTCCA 
GGTGATCCTG GACTTCCAGG CCAACCAGGC CTACCAGGAA TACCTGGTAG CAAAGGAGAA 
CCAGGTATCC CTGGGATTGG GCCTCCAGGA CCACCTGGTC CCAAAGGTTT TCCAGGAATT 
CCTGGACCTC CAGGACCTCC TGGGGCCCCT GGAAGAATGG GCCCAGAAGG GCCTCCTGGG 
TTACCGGGCT TTCCAGGACC CAAGGGAGAG CCAGGATTTG GGTTACCTGG GCCACCTGGG 
CCACCAGGAT TCCCAGGTTT CAAAGGAACA CCTGGTCCAA AAGGTGATCG TGGTTTACCA 
GGACCTCCAG GTCCTCCAGG ACACACTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAT 
GCTGGACCAA ATGGACAACC TGGCCCAATA GGGCCACCAG GGCTGCCAGG AATTGGTCTT 
CAGGGACCAC CAGGACCACC AGGGACTCCA GGGCCAATAG GCCAACCTGG CTTACATGGA 
ATACCAGGAG AGAAGGGAGA TCCAGGACCT CCTGGGCTTG ATGTTCCAGG ACTCCCTGGA 
GAGAGAGGTA GTCCAGGGCT CCCTGGAGCC CCTGGTCTTA TAGGACCTCC AGGATCACCT 
GGTATCCCTG GAAGAGCAGG TGTCCCTGGA TTTCCAGGTG TCAAAGGCGA AATGGGTATG 
ATGGGACCTC CAGGCCCACC GGGACCTTTG GGTACTCCTG GCAGGAGTGG TGCTCCTGGT 
CTTAAAGGTG ATGACGGCAT GCAAGGTCAG CCAGGTCTCC CTGGCCCTGT AGGAGAAAAG 
GGTGGTAAGG GAGAACCTGG CCTTCCAGGT CCTCCTGGAC CAATGCGTCC AGATTTCCTG 
GGATCAAAAG GAGAAAAGGG TGATCCTGGC TTACCAGGTA TTCCTGGACT TTCCGGACCA 
AAAGGCTACC AGGGATTACC TGGAGACCCA GGGCAACCTG GGCTGAGTGG ACAGCCGGGA 
TCACCAGGAC TACCAGGTCC CAAAGGTACT CCTGGTCTCC CTGGTCCACC AGGACTTACC 
GGACCTCCTG GACCTAAAGG AAACATTGGC GACATGGGTT TTCCTGGCCC TCAAGGTGTA 
GATGGGCCTC CTGGACCTCC TGGAGGTCCT GGACAACCTG GCTCTCCCGG GTTGCCTGGC 
CAGAAAGGAA GTAAAGGAGA ACCTGGAGTT TCAGGCATTG GTCTCCCTGG TCTTCCTGGT 
CCAAAGGGTG AACCTGGTCT GCCTGGATAT CCAGGAAACC CAGGTATCAA AGGTTCTATG 
GGCGAGGCTG GTCTGCCTGG CTTACCAGGA AGCCCTGGAG CAAAAGGTCA ACCCGGCCTG 
CCCGGATTCC CAGGAACACC AGGGCTTCCT GGACCAAAGG GTATGAATGG TCCTCCTGGT 
AACCCTGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGGCGTCC TGGACCACCA 
GGACCTCCTG GTGAAAAAGG CAAGCCAGGT CAAGATGGCA TTCCTGGACC AGCAGGACAA 
AAGGGAGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CTCCTGGACT CCCAGGACTT 
TCTGGTCAAA AGGGTGATGG AGGAGCCCCT GGCCTTCCAG GAAATCCTGG CCTTCCAGGT 
TCAAAGGGTG AACCAGGCTT TCAGGGATTC CCTGGTATGC CAGGTCCCCC AGGTCCCCCT 
GGTTCTCCTG GCCCATCTTT GGAAGGTCCC AAAGGAAACC CTGGGCCTCA AGGCCCTCCT 
GGGAGACCAG GTTTTCAAGG TCCACCTGGT ACAGAAGGTC CTCGTGGCCC CCCTGGAAAT 
GGAGGTATTA AAGGAGAGAG GGGAAATCCA GGTCCACCTG GGCAACCAGG CTTACCTGGC 
TTGAAAGGAG ATCAAGGACC ACCAGGCCTT CCGGGTAACC CTGGCCGACC AGGTCTTAAT 
GGAATGAAAG GAGATCCTGG TCTCCCTGGT GTTCCAGGAT TCCCAGGAAT GAAAGGACCC 
ATTGGAGTAC CAGGCTCAAC TGGCCCTGAT GGGGAACCAG GTCTTACGGG GCCCCCAGGT 
CCTCCTGGGT TGCCTGGTCC TTCAGGACAG AGTATTGTAA TCAAAGGAGA TCCTGGACCT 
CCAGGAATTC CTGGACAACC TGGATTAAAA GGTCTACCAG GACTTCCAGG CCCTCAAGGT 
CTACCAGGTC CAATTGGCCC TCCAGGAGAT CCAGGACGCA ATGGACTCCC AGGCTTTGAT 
GGTGCAGGAG GGCGCAAAGG AGACCCAGGC CTGCCGGGGC AGCCAGGTAG CCGTGGATTG 
GATGGTCCCC CAGGACCAGA TGGATTGCAA GGACCTCCAG GGCCCCCGGG AACCACCTCT 
GTTGCCCATG GATTCCTCAT CACACGTCAC AGTCAGACAA CAGATGCACC ACAATGCCCA 
CAGGGAACAG TCCATATCTA TGATGGCTTT TCTCTCTTAT ATGTACAAGG AAATAAAAGA 
GCCCATGGTC AAGACTTGGG GACAGCTGGC AGCTGCCTTC GTCGCTTCAG TACCATGCCT 
TTCATGTTCT GCAACATTAA CAACGTTTGT AACTTTGCTT CAAGAAATGA CTATTCTTAC 
TGGCTTTCCA CCCCAGAGCC CATGCCAATG AACATGGAAC CCCTGAAGGG CCAGAGCATC 
CAGCCATTCA TTAGTCGATG TGCAGTATGT GAGGCACCAG CTGTGGTGAT TGCAGTTCAC 
AGCCAGACTA TTCAGATTCC TCACTGTCCT CAGGGGTGGG ACTCTCTGTG GATTGGCTAT 
TCATTCATGA TGCATACAAG TGCAGGAGCA GAAGGCTCTG GCCAAGCCCT AGCCTCTCCT 
GGCTCCTGTT TGGAAGAATT TCGTTCAGCT CCCTTCATCG AATGTCATGG ACGAGGGACA 
TGCAATTACT ATGCCAATTC TTACAGCTTT TGGCTGGCCA CTGTTGATAT GTCAGACATG 
TTCAGTAAAC CTCAGTCAGA GACACTGAAA GCAGGCGACT TGAGGACACG TATTAGCCGA 
TGTCAAGTAT GCATGAAAAG GACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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Col4a5 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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