Nsmaf cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nsmaf
Entrez Gene ID	353233
Full Name	neutral sphingomyelinase activation associated factor
Synonyms	FAN
General protein information	Preferred Names neutral sphingomyelinase activation associated factor Names protein FAN factor associated with neutral sphingomyelinase activation neutral sphingomyelinase (N-SMase) activation associated factor
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 5 Map Location: 5q12
Summary	cytopasmic protein; mediates activation of sphingomyelinase and apoptosis [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_008763544.2	XP_008761766.1	protein FAN isoform X1
XM_017593446.1	XP_017448935.1	protein FAN isoform X3
XM_008763545.2	XP_008761767.1	protein FAN isoform X2
NM_181389.2	NP_852054.1	protein FAN

Pathways from BioSystems

Homologies

Bos taurus (cattle)	NSMAF	XP_002692713.2
Homo sapiens (human)	NSMAF	NP_003571.2
Pan troglodytes (chimpanzee)	NSMAF	XP_001156895.1
Mus musculus (house mouse)	Nsmaf	NP_035075.2
Rattus norvegicus (Norway rat)	Nsmaf	NP_852054.1
Canis lupus familiaris (dog)	NSMAF	XP_005638205.1
Danio rerio (zebrafish)	nsmaf	XP_003199050.1
	NSMAF	XP_001088417.1
Gallus gallus (chicken)	NSMAF	XP_003640887.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000429	XP_310670.5
Xenopus tropicalis (tropical clawed frog)	nsmaf	NP_001121464.1

Gene Ontology
Bibliography

Related articles in PubMed

Neutral sphingomyelinase, NADPH oxidase and reactive oxygen species. Role in acute hypoxic pulmonary vasoconstriction.
Frazziano G, Moreno L, Moral-Sanz J, Menendez C, Escolano L, Gonzalez C, Villamor E, Alvarez-Sala JL, Cogolludo AL, Perez-Vizcaino F
Journal of cellular physiology226(10)2633-40(2011 Oct)

The group VIA calcium-independent phospholipase A2 participates in ER stress-induced INS-1 insulinoma cell apoptosis by promoting ceramide generation via hydrolysis of sphingomyelins by neutral sphingomyelinase.
Lei X, Zhang S, Bohrer A, Bao S, Song H, Ramanadham S
Biochemistry46(35)10170-85(2007 Sep)

Factor associated with neutral sphingomyelinase activation and its role in cardiac cell death.
O'Brien NW, Gellings NM, Guo M, Barlow SB, Glembotski CC, Sabbadini RA
Circulation research92(6)589-91(2003 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We propose an integrated signalling pathway for hypoxic pulmonary vasoconstriction which includes nSMase-PKCzeta-NADPH oxidase as a necessary step required for reactive oxygen species production.
Title: Neutral sphingomyelinase, NADPH oxidase and reactive oxygen species. Role in acute hypoxic pulmonary vasoconstriction.

These findings indicate that iPLA2beta activation participates in ER stress-induced INS-1 cell apoptosis by promoting ceramide generation via NSMase-catalyzed hydrolysis of sphingomyelins
Title: The group VIA calcium-independent phospholipase A2 participates in ER stress-induced INS-1 insulinoma cell apoptosis by promoting ceramide generation via hydrolysis of sphingomyelins by neutral sphingomyelinase.

The following Nsmaf gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nsmaf cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa04350	NM_181389.2 Latest version!	Rattus norvegicus neutral sphingomyelinase activation associated factor (Nsmaf), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa47648	XM_008763544.2 Latest version!	Rattus norvegicus neutral sphingomyelinase activation associated factor (Nsmaf), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa47649	XM_008763545.2 Latest version!	Rattus norvegicus neutral sphingomyelinase activation associated factor (Nsmaf), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa61268	XM_017593446.1 Latest version!	Rattus norvegicus neutral sphingomyelinase activation associated factor (Nsmaf), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nsmaf ( NM_181389.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_852054.1 Rattus norvegicus neutral sphingomyelinase activation associated factor (Nsmaf), mRNA.

Price & Availability↑

CloneID	ORa04350
Clone ID Related Accession (Same CDS sequence)	NM_181389.2
Accession Version	NM_181389.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2763bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-31
Organism	Rattus norvegicus(Norway rat)
Product	protein FAN
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY163240.1. On Jun 3, 2003 this sequence version replaced NM_181389.1. ##Evidence-Data-START## Transcript exon combination :: AY163240.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGCGTTCA CCCGGAAGAG GCAGCGCGAG CAGCAGCTGC AGCTCTACTC CAAGGAGAGG 
TTCTCCTTAC TGCTGCTCAA CTTGGAGGAG TACTACTTCG AGCAGCATAC GGCTTTCCAT 
GTCCAGCACC AGGGCAGCCC CGAGGAAAGG AAAATCAGAG GCTCCTTAAA GATATGTTCA 
AAATCAGTGA TTTTTGAACC AGATGCAATA TCGCAGCCCA TTCTTAAGAT TCCTTTGAGA 
GACTGTTTAA AAATAGGAAA ACATGGAGAA AATGGAGCCA ATAAACACTT TGCAAAGGCA 
AAATCTTTGG GTATTTCACT CATTTCCAGT CAGGTATATT TCATTAAGGA GCACAACATC 
GTGGCACCGT ATAAGATAGA ACGGGGAAAA ATGGAGTATG TCTTCCAACT GGAAGTTTCT 
GGGAAGGTGG AAGATGTTGT GGAGACATTG CTACAGCTGC ACAGAGCATC CTGCCTTGAT 
AAACTTGGTG ACCAAATGGC CATGATAACA GCTATCTTGC AGTCACGTTT GGCTAGGACA 
TCATTTGACA AAAACAGGTT CCAAAGCGTT TCAGAAAAGC TGCACATGGA GTGCAAAGCA 
GAGATGATGA CGCCTCTGGT GACGAACTCT GGGCATGTGT GCATTACTGA CACCAACCTG 
TATTTCCAGC CTCTCAACGG GTACCCGAAA CCTGTGGTCC AGATAACGCT CCAAGATGTC 
CGGCGCATTT ACAAAAGGAG GCACGGCCTC ATGCCTTTGG GCTTGGAAGT GTTTTGCACA 
GAAGATGACC TGTGTTCTGA CATATACCTA AAGTTCTATG AGCCTCAAGA CAGAGATGAC 
CTCTATTTTT ATATTGCCAC GTACCTAGAA CACCACGTGG CAGAACACAC TGCGGAGAGC 
TACATGCTGC AGTGGCAGCG AGGGCACCTG TCCAACTACC AGTACCTGCT CCACTTCAAC 
AACCTGGCCG ACCGCGGCTG CAATGACCTG TCCCAGTACC CTGTGTTTCC ATGGGTAATA 
AGTGACTACT CCAACCCAGA GCTAGATTTG TCAAATCCCG CAACCTTCCG AGATCTTAGT 
AAGCCAGTGG GAGCCCTAAA TCCAGAGAGG CTGGAGAGAC TGCTGACTCG ATACCAGGAA 
ATGCCTGAGC CGAAGTTCAT GTATGGGAGC CACTACTCTT CCCCAGGCTA TGTGCTCTTT 
TACCTCGTTA GGATCGCACC AGAATATATG CTGTGTCTAC AAAATGGAAG ATTTGATAAT 
GCAGATAGGA TGTTCAACAG CATTGTAGAA ACCTGGAAAA ACTGTTTGGA TGGTGCAACG 
AACTTCAAAG AGTTGATCCC AGAATTCTAT GATGAAGACG CCAGCTTCTT GATCAATAGT 
CTGAAGTTGG ATCTGGGGAA GAGACAAGGA GGGCAGATGG TTGACGACGT GGAGCTTCCT 
GCATGGGCCT CAAGTCCCCA AGACTTTCTC CAGAAGAACA AGGACGCCTT GGAAAGCAGT 
TATGTGTCTG AGCACCTTCA TGAGTGGATT GATCTGATAT TTGGCTATAA GCAAAAAGGG 
AGTGAGGCCA TAGGGGCCCA TAACGTGTTT CACCCCCTGA CCTATGAAGG CGGTGTAGAC 
CTGAACAGCA TTGAGGATCC CGATGACAAA GTAGCCATGC TGACCCAAAT TTTGGAATTT 
GGGCAGACAC CAAAACAGCT GTTTGTGACA CCACATCCTC GAAGGATCAC CCCCAAGTTT 
AAAAGTTTGT CCCAAGCGTC CAGCTACAAT GCTTCTATGA CAGACTCCCC AGTTTCTCCA 
GGGGAGGAGT CATTTGAAGA CCTGACTGAA GAGAGCAAAA CTCTGGCCTG GAATAACATC 
ACCAAGTTGC AGCTACATGA GCAGTACAAA ATCCACAAAG AGGCGGTTAC AGGGATAGCA 
GTGTCTTGCA ATGGGTCTTC AGTCTTTACC ACCTCACAAG ATTCCACTTT GAAGATGTTT 
TCTAAGGAAT CCAAAATGTT ACAAAGAAGT ATATCATTTT CAATATGGGC TTTATCGTCT 
TGTTTACTTT TGCCAGGAGA CACCACTGTC ATAAGTTCTT CTTGGGATAA CAACGTCTAT 
TTTTATTCCA TAGCATTTGG AAGACGCCAA GACACACTGA TGGGACATGA CGATGCTGTC 
AGTAAGATCT GTTGGCATAA TGACAGGCTG TACTCTGCAT CTTGGGACTC TACTGTGAAG 
GTGTGGTCCG GCGTTCCTGC AGAAATGCCA GGCACAAAAA GACACCAGTT TGACTTACTG 
GCTGAGCTTG AGCATGATGT CAGTGTCAAT ACGATCAATT TAAATGCTGT GAGTACACTG 
CTAGTTTCTG GCACCAAGGA AGGCCTGGTG AATATTTGGG ACCTCACTAC AGCCACTCTA 
TTACACCAGA TTTCATGCCA CTCAGGGACA GTATGTGATG CTGCTTTTAG CCCAGACAGT 
CGCCACGTCC TCAGCACAGG AGTAGACGGC TGCCTGAACG TGATTGATGT GCAGACGGGC 
ATGCTCATTT CCTCCATGGC CTCAGAGGAG CCGCAGAGGT GCTTTGTCTG GGATGGAAAC 
TCTGTCGTAT CTGGAAGTCG GACTGGTGAG CTGCTTGTCT GGGACCTCCT TGGAGCAAAA 
GTCAGTGAGC GGATACAGGG CCACACAGGT GCCGTGACGT GCATACGGAT GAATGAACAG 
TGTAGCAGCA TCATCACCGG AGGGGAAGAC AGACAAGTTA TGTTCTGGAA ACTGCAGTAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_852054.1
CDS	9..2771
Translation	MAFTRKRQREQQLQLYSKERFSLLLLNLEEYYFEQHTAFHVQHQGSPEERKIRGSLKICSKSVIFEPDAISQPILKIPLRDCLKIGKHGENGANKHFAKAKSLGISLISSQVYFIKEHNIVAPYKIERGKMEYVFQLEVSGKVEDVVETLLQLHRASCLDKLGDQMAMITAILQSRLARTSFDKNRFQSVSEKLHMECKAEMMTPLVTNSGHVCITDTNLYFQPLNGYPKPVVQITLQDVRRIYKRRHGLMPLGLEVFCTEDDLCSDIYLKFYEPQDRDDLYFYIATYLEHHVAEHTAESYMLQWQRGHLSNYQYLLHFNNLADRGCNDLSQYPVFPWVISDYSNPELDLSNPATFRDLSKPVGALNPERLERLLTRYQEMPEPKFMYGSHYSSPGYVLFYLVRIAPEYMLCLQNGRFDNADRMFNSIVETWKNCLDGATNFKELIPEFYDEDASFLINSLKLDLGKRQGGQMVDDVELPAWASSPQDFLQKNKDALESSYVSEHLHEWIDLIFGYKQKGSEAIGAHNVFHPLTYEGGVDLNSIEDPDDKVAMLTQILEFGQTPKQLFVTPHPRRITPKFKSLSQASSYNASMTDSPVSPGEESFEDLTEESKTLAWNNITKLQLHEQYKIHKEAVTGIAVSCNGSSVFTTSQDSTLKMFSKESKMLQRSISFSIWALSSCLLLPGDTTVISSSWDNNVYFYSIAFGRRQDTLMGHDDAVSKICWHNDRLYSASWDSTVKVWSGVPAEMPGTKRHQFDLLAELEHDVSVNTINLNAVSTLLVSGTKEGLVNIWDLTTATLLHQISCHSGTVCDAAFSPDSRHVLSTGVDGCLNVIDVQTGMLISSMASEEPQRCFVWDGNSVVSGSRTGELLVWDLLGAKVSERIQGHTGAVTCIRMNEQCSSIITGGEDRQVMFWKLQY

Target ORF information:

RefSeq Version	NM_181389.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus neutral sphingomyelinase activation associated factor (Nsmaf), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_181389.2

ORF Insert Sequence:

ATGGCGTTCA CCCGGAAGAG GCAGCGCGAG CAGCAGCTGC AGCTCTACTC CAAGGAGAGG 
TTCTCCTTAC TGCTGCTCAA CTTGGAGGAG TACTACTTCG AGCAGCATAC GGCTTTCCAT 
GTCCAGCACC AGGGCAGCCC CGAGGAAAGG AAAATCAGAG GCTCCTTAAA GATATGTTCA 
AAATCAGTGA TTTTTGAACC AGATGCAATA TCGCAGCCCA TTCTTAAGAT TCCTTTGAGA 
GACTGTTTAA AAATAGGAAA ACATGGAGAA AATGGAGCCA ATAAACACTT TGCAAAGGCA 
AAATCTTTGG GTATTTCACT CATTTCCAGT CAGGTATATT TCATTAAGGA GCACAACATC 
GTGGCACCGT ATAAGATAGA ACGGGGAAAA ATGGAGTATG TCTTCCAACT GGAAGTTTCT 
GGGAAGGTGG AAGATGTTGT GGAGACATTG CTACAGCTGC ACAGAGCATC CTGCCTTGAT 
AAACTTGGTG ACCAAATGGC CATGATAACA GCTATCTTGC AGTCACGTTT GGCTAGGACA 
TCATTTGACA AAAACAGGTT CCAAAGCGTT TCAGAAAAGC TGCACATGGA GTGCAAAGCA 
GAGATGATGA CGCCTCTGGT GACGAACTCT GGGCATGTGT GCATTACTGA CACCAACCTG 
TATTTCCAGC CTCTCAACGG GTACCCGAAA CCTGTGGTCC AGATAACGCT CCAAGATGTC 
CGGCGCATTT ACAAAAGGAG GCACGGCCTC ATGCCTTTGG GCTTGGAAGT GTTTTGCACA 
GAAGATGACC TGTGTTCTGA CATATACCTA AAGTTCTATG AGCCTCAAGA CAGAGATGAC 
CTCTATTTTT ATATTGCCAC GTACCTAGAA CACCACGTGG CAGAACACAC TGCGGAGAGC 
TACATGCTGC AGTGGCAGCG AGGGCACCTG TCCAACTACC AGTACCTGCT CCACTTCAAC 
AACCTGGCCG ACCGCGGCTG CAATGACCTG TCCCAGTACC CTGTGTTTCC ATGGGTAATA 
AGTGACTACT CCAACCCAGA GCTAGATTTG TCAAATCCCG CAACCTTCCG AGATCTTAGT 
AAGCCAGTGG GAGCCCTAAA TCCAGAGAGG CTGGAGAGAC TGCTGACTCG ATACCAGGAA 
ATGCCTGAGC CGAAGTTCAT GTATGGGAGC CACTACTCTT CCCCAGGCTA TGTGCTCTTT 
TACCTCGTTA GGATCGCACC AGAATATATG CTGTGTCTAC AAAATGGAAG ATTTGATAAT 
GCAGATAGGA TGTTCAACAG CATTGTAGAA ACCTGGAAAA ACTGTTTGGA TGGTGCAACG 
AACTTCAAAG AGTTGATCCC AGAATTCTAT GATGAAGACG CCAGCTTCTT GATCAATAGT 
CTGAAGTTGG ATCTGGGGAA GAGACAAGGA GGGCAGATGG TTGACGACGT GGAGCTTCCT 
GCATGGGCCT CAAGTCCCCA AGACTTTCTC CAGAAGAACA AGGACGCCTT GGAAAGCAGT 
TATGTGTCTG AGCACCTTCA TGAGTGGATT GATCTGATAT TTGGCTATAA GCAAAAAGGG 
AGTGAGGCCA TAGGGGCCCA TAACGTGTTT CACCCCCTGA CCTATGAAGG CGGTGTAGAC 
CTGAACAGCA TTGAGGATCC CGATGACAAA GTAGCCATGC TGACCCAAAT TTTGGAATTT 
GGGCAGACAC CAAAACAGCT GTTTGTGACA CCACATCCTC GAAGGATCAC CCCCAAGTTT 
AAAAGTTTGT CCCAAGCGTC CAGCTACAAT GCTTCTATGA CAGACTCCCC AGTTTCTCCA 
GGGGAGGAGT CATTTGAAGA CCTGACTGAA GAGAGCAAAA CTCTGGCCTG GAATAACATC 
ACCAAGTTGC AGCTACATGA GCAGTACAAA ATCCACAAAG AGGCGGTTAC AGGGATAGCA 
GTGTCTTGCA ATGGGTCTTC AGTCTTTACC ACCTCACAAG ATTCCACTTT GAAGATGTTT 
TCTAAGGAAT CCAAAATGTT ACAAAGAAGT ATATCATTTT CAATATGGGC TTTATCGTCT 
TGTTTACTTT TGCCAGGAGA CACCACTGTC ATAAGTTCTT CTTGGGATAA CAACGTCTAT 
TTTTATTCCA TAGCATTTGG AAGACGCCAA GACACACTGA TGGGACATGA CGATGCTGTC 
AGTAAGATCT GTTGGCATAA TGACAGGCTG TACTCTGCAT CTTGGGACTC TACTGTGAAG 
GTGTGGTCCG GCGTTCCTGC AGAAATGCCA GGCACAAAAA GACACCAGTT TGACTTACTG 
GCTGAGCTTG AGCATGATGT CAGTGTCAAT ACGATCAATT TAAATGCTGT GAGTACACTG 
CTAGTTTCTG GCACCAAGGA AGGCCTGGTG AATATTTGGG ACCTCACTAC AGCCACTCTA 
TTACACCAGA TTTCATGCCA CTCAGGGACA GTATGTGATG CTGCTTTTAG CCCAGACAGT 
CGCCACGTCC TCAGCACAGG AGTAGACGGC TGCCTGAACG TGATTGATGT GCAGACGGGC 
ATGCTCATTT CCTCCATGGC CTCAGAGGAG CCGCAGAGGT GCTTTGTCTG GGATGGAAAC 
TCTGTCGTAT CTGGAAGTCG GACTGGTGAG CTGCTTGTCT GGGACCTCCT TGGAGCAAAA 
GTCAGTGAGC GGATACAGGG CCACACAGGT GCCGTGACGT GCATACGGAT GAATGAACAG 
TGTAGCAGCA TCATCACCGG AGGGGAAGAC AGACAAGTTA TGTTCTGGAA ACTGCAGTAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nsmaf ( XM_008763544.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008761766.1 Rattus norvegicus neutral sphingomyelinase activation associated factor (Nsmaf), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa47648
Clone ID Related Accession (Same CDS sequence)	XM_008763544.2
Accession Version	XM_008763544.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2763bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	protein FAN isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008763544.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTTCA CCCGGAAGAG GCAGCGCGAG CAGCAGCTGC AGCTCTACTC CAAGGAGAGG 
TTCTCCTTAC TGCTGCTCAA CTTGGAGGAG TACTACTTCG AGCAGCATAC GGCTTTCCAT 
GTCCAGCACC AGGGCAGCCC CGAGGAAAGG AAAATCAGAG GCTCCTTAAA GATATGTTCA 
AAATCAGTGA TTTTTGAACC AGATGCAATA TCGCAGCCCA TTCTTAAGAT TCCTTTGAGA 
GACTGTTTAA AAATAGGAAA ACATGGAGAA AATGGAGCCA ATAAACACTT TGCAAAGGCA 
AAATCTTTGG GTATTTCACT CATTTTCAGT CAGGTATATT TCATTAAGGA GCACAACATC 
GTGGCACCGT ATAAGATAGA ACGGGGAAAA ATGGAGTATG TCTTCCAACT GGAAGTTTCT 
GGGAAGGTGG AAGATGTTGT GGAGACATTG CTACAGCTGC ACAGAGCATC CTGCCTTGAT 
AAACTTGGTG ACCAAATGGC CATGATAACA GCTATCTTGC AGTCACGTTT GGCTAGGACA 
TCATTTGACA AAAACAGGTT CCAAAGCGTT TCAGAAAAGC TGCACATGGA GTGCAAAGCA 
GAGATGGTGA CGCCTCTGGT GACGAACCCT GGGCATGTGT GCATTACTGA CACCAACCTG 
TATTTCCAGC CTCTCAACGG GTACCCGAAA CCTGTGGTCC AGATAACGCT CCAAGATGTC 
CGGCGCATTT ACAAAAGGAG GCACGGCCTC ATGCCTTTGG GCTTGGAAGT GTTTTGCACA 
GAAGATGACC TGTGTTCTGA CATATACCTA AAGTTCTATG AGCCTCAAGA CAGAGATGAC 
CTCTATTTTT ATATTGCCAC GTACCTAGAA CACCACGTGG CAGAACACAC TGCGGAGAGC 
TACATGCTGC AGTGGCAGCG AGGGCACCTG TCCAACTACC AGTACCTGCT CCACCTCAAC 
AACCTGGCCG ACCGCAGCTG CAATGACCTG TCCCAGTACC CTGTGTTTCC ATGGGTAATA 
AGTGACTACT CCAGCCCAGA GCTAGATTTG TCAAATCCTG CAACCTTCCG AGATCTTAGT 
AAGCCAGTGG GAGCCCTAAA TCCAGAGAGG CTGGAGAGAC TGCTGACTCG ATACCAGGAA 
ATGCCTGAGC CGAAGTTCAT GTATGGGAGC CACTACTCTT CCCCAGGCTA TGTGCTCTTT 
TACCTCGTTA GGATCGCACC AGAATATATG CTGTGTCTAC AAAATGGAAG ATTTGATAAT 
GCAGATAGGA TGTTCAACAG CATTGCAGAA ACCTGGAAAA ACTGTTTGGA TGGTGCGACG 
GACTTCAAAG AGTTGATCCC AGAATTCTAT GATGAAGACG CCAGCTTCTT GATCAATAGT 
CTGAAGTTGG ATCTGGGGAA GAGACAAGGA GGGCAGATGG TTGACGACGT GGAGCTTCCT 
GCATGGGCCT CAAGTCCCCA AGACTTTCTC CAGAAGAACA AGGACGCCTT GGAAAGCAGT 
TATGTGTCTG AGCACCTTCA TGAGTGGATT GATCTGATAT TTGGCTATAA GCAAAAAGGG 
AGTGAGGCCA TAGGGGCCCA TAACGTGTTT CACCCCCTGA CCTATGAAGG CGGTGTAGAC 
CTGAACAGCA TTGAGGATCC CGATGACAAA GTAGCCATGC TGACCCAAAT TTTGGAATTT 
GGGCAGACAC CAAAACAGCT GTTTGTGACA CCACATCCTC GAAGGATCAC CCCCAAGTTT 
AAAAGTTTGT CCCAAGCGTC CAGCTACAAT GCTTCTATGA CAGACTCCCC AGTTTCTCCA 
GGGGAGGAGT CATTTGAAGA CCTGACTGAA GAGAGCAAAA CTCTGGCCTG GAATAACATC 
ACCAAGTTGC AGCTACATGA GCAGTACAAA ATCCACAAAG AGGCGGTTAC AGGGATAGCA 
GTGTCTTGCA ATGGGTCTTC AGTCTTTACC ACCTCACAAG ATTCCACTTT GAAGATGTTT 
TCTAAGGAAT CCAAAATGTT ACAAAGAAGT ATATCATTTT CAAATATGGC TTTATCGTCT 
TGTTTACTTT TGCCAGGAGA CACCACTGTC ATAAGTTCTT CTTGGGATAA CAACGTCTAT 
TTTTATTCCA TAGCATTTGG AAGACGCCAA GACACACTGA TGGGACATGA CGATGCTGTC 
AGTAAGATCT GTTGGCATAA TGACAGGCTG TACTCTGCAT CTTGGGACTC TACTGTGAAG 
GTGTGGTCCG GCGTTCCTGC AGAAATGCCA GGCACAAAAA GACACCAGTT TGACTTACTG 
GCTGAGCTTG AGCATGATGT CAGTGTCAAT ACGATCAATT TAAATGCTGT GAGTACACTG 
CTAGTTTCTG GCACCAAGGA AGGCCTGGTG AATATTTGGG ACCTCACTAC AGCCACTCTA 
TTACACCAGA TTTCATGCCA CTCAGGGACA GTATGTGATG CTGCTTTTAG CCCAGACAGT 
CGCCACGTCC TCAGCACAGG AGTAGACGGC TGCCTGAACG TGATTGATGT GCAGACGGGC 
ATGCTCATTT CCTCCATGGC CTCAGAGGAG CCGCAGAGGT GCTTTGTCTG GGATGGAAAC 
TCTGTCTTAT CTGGAAGTCG GACTGGTGAG CTGCTTGTCT GGGACCTCCT TGGAGCAAAA 
GTCAGTGAGC GGATACAGGG CCACACAGGT GCCGTGACGT GCATATGGAT GAATGAACAG 
TGTAGCAGCA TCATCACCGG AGGGGAAGAC AGACAAGTTA TGTTCTGGAA ACTGCAGTAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008761766.1
CDS	1071..3833
Translation	MAFTRKRQREQQLQLYSKERFSLLLLNLEEYYFEQHTAFHVQHQGSPEERKIRGSLKICSKSVIFEPDAISQPILKIPLRDCLKIGKHGENGANKHFAKAKSLGISLIFSQVYFIKEHNIVAPYKIERGKMEYVFQLEVSGKVEDVVETLLQLHRASCLDKLGDQMAMITAILQSRLARTSFDKNRFQSVSEKLHMECKAEMVTPLVTNPGHVCITDTNLYFQPLNGYPKPVVQITLQDVRRIYKRRHGLMPLGLEVFCTEDDLCSDIYLKFYEPQDRDDLYFYIATYLEHHVAEHTAESYMLQWQRGHLSNYQYLLHLNNLADRSCNDLSQYPVFPWVISDYSSPELDLSNPATFRDLSKPVGALNPERLERLLTRYQEMPEPKFMYGSHYSSPGYVLFYLVRIAPEYMLCLQNGRFDNADRMFNSIAETWKNCLDGATDFKELIPEFYDEDASFLINSLKLDLGKRQGGQMVDDVELPAWASSPQDFLQKNKDALESSYVSEHLHEWIDLIFGYKQKGSEAIGAHNVFHPLTYEGGVDLNSIEDPDDKVAMLTQILEFGQTPKQLFVTPHPRRITPKFKSLSQASSYNASMTDSPVSPGEESFEDLTEESKTLAWNNITKLQLHEQYKIHKEAVTGIAVSCNGSSVFTTSQDSTLKMFSKESKMLQRSISFSNMALSSCLLLPGDTTVISSSWDNNVYFYSIAFGRRQDTLMGHDDAVSKICWHNDRLYSASWDSTVKVWSGVPAEMPGTKRHQFDLLAELEHDVSVNTINLNAVSTLLVSGTKEGLVNIWDLTTATLLHQISCHSGTVCDAAFSPDSRHVLSTGVDGCLNVIDVQTGMLISSMASEEPQRCFVWDGNSVLSGSRTGELLVWDLLGAKVSERIQGHTGAVTCIWMNEQCSSIITGGEDRQVMFWKLQY

Target ORF information:

RefSeq Version	XM_008763544.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus neutral sphingomyelinase activation associated factor (Nsmaf), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008763544.2

ORF Insert Sequence:

ATGGCGTTCA CCCGGAAGAG GCAGCGCGAG CAGCAGCTGC AGCTCTACTC CAAGGAGAGG 
TTCTCCTTAC TGCTGCTCAA CTTGGAGGAG TACTACTTCG AGCAGCATAC GGCTTTCCAT 
GTCCAGCACC AGGGCAGCCC CGAGGAAAGG AAAATCAGAG GCTCCTTAAA GATATGTTCA 
AAATCAGTGA TTTTTGAACC AGATGCAATA TCGCAGCCCA TTCTTAAGAT TCCTTTGAGA 
GACTGTTTAA AAATAGGAAA ACATGGAGAA AATGGAGCCA ATAAACACTT TGCAAAGGCA 
AAATCTTTGG GTATTTCACT CATTTTCAGT CAGGTATATT TCATTAAGGA GCACAACATC 
GTGGCACCGT ATAAGATAGA ACGGGGAAAA ATGGAGTATG TCTTCCAACT GGAAGTTTCT 
GGGAAGGTGG AAGATGTTGT GGAGACATTG CTACAGCTGC ACAGAGCATC CTGCCTTGAT 
AAACTTGGTG ACCAAATGGC CATGATAACA GCTATCTTGC AGTCACGTTT GGCTAGGACA 
TCATTTGACA AAAACAGGTT CCAAAGCGTT TCAGAAAAGC TGCACATGGA GTGCAAAGCA 
GAGATGGTGA CGCCTCTGGT GACGAACCCT GGGCATGTGT GCATTACTGA CACCAACCTG 
TATTTCCAGC CTCTCAACGG GTACCCGAAA CCTGTGGTCC AGATAACGCT CCAAGATGTC 
CGGCGCATTT ACAAAAGGAG GCACGGCCTC ATGCCTTTGG GCTTGGAAGT GTTTTGCACA 
GAAGATGACC TGTGTTCTGA CATATACCTA AAGTTCTATG AGCCTCAAGA CAGAGATGAC 
CTCTATTTTT ATATTGCCAC GTACCTAGAA CACCACGTGG CAGAACACAC TGCGGAGAGC 
TACATGCTGC AGTGGCAGCG AGGGCACCTG TCCAACTACC AGTACCTGCT CCACCTCAAC 
AACCTGGCCG ACCGCAGCTG CAATGACCTG TCCCAGTACC CTGTGTTTCC ATGGGTAATA 
AGTGACTACT CCAGCCCAGA GCTAGATTTG TCAAATCCTG CAACCTTCCG AGATCTTAGT 
AAGCCAGTGG GAGCCCTAAA TCCAGAGAGG CTGGAGAGAC TGCTGACTCG ATACCAGGAA 
ATGCCTGAGC CGAAGTTCAT GTATGGGAGC CACTACTCTT CCCCAGGCTA TGTGCTCTTT 
TACCTCGTTA GGATCGCACC AGAATATATG CTGTGTCTAC AAAATGGAAG ATTTGATAAT 
GCAGATAGGA TGTTCAACAG CATTGCAGAA ACCTGGAAAA ACTGTTTGGA TGGTGCGACG 
GACTTCAAAG AGTTGATCCC AGAATTCTAT GATGAAGACG CCAGCTTCTT GATCAATAGT 
CTGAAGTTGG ATCTGGGGAA GAGACAAGGA GGGCAGATGG TTGACGACGT GGAGCTTCCT 
GCATGGGCCT CAAGTCCCCA AGACTTTCTC CAGAAGAACA AGGACGCCTT GGAAAGCAGT 
TATGTGTCTG AGCACCTTCA TGAGTGGATT GATCTGATAT TTGGCTATAA GCAAAAAGGG 
AGTGAGGCCA TAGGGGCCCA TAACGTGTTT CACCCCCTGA CCTATGAAGG CGGTGTAGAC 
CTGAACAGCA TTGAGGATCC CGATGACAAA GTAGCCATGC TGACCCAAAT TTTGGAATTT 
GGGCAGACAC CAAAACAGCT GTTTGTGACA CCACATCCTC GAAGGATCAC CCCCAAGTTT 
AAAAGTTTGT CCCAAGCGTC CAGCTACAAT GCTTCTATGA CAGACTCCCC AGTTTCTCCA 
GGGGAGGAGT CATTTGAAGA CCTGACTGAA GAGAGCAAAA CTCTGGCCTG GAATAACATC 
ACCAAGTTGC AGCTACATGA GCAGTACAAA ATCCACAAAG AGGCGGTTAC AGGGATAGCA 
GTGTCTTGCA ATGGGTCTTC AGTCTTTACC ACCTCACAAG ATTCCACTTT GAAGATGTTT 
TCTAAGGAAT CCAAAATGTT ACAAAGAAGT ATATCATTTT CAAATATGGC TTTATCGTCT 
TGTTTACTTT TGCCAGGAGA CACCACTGTC ATAAGTTCTT CTTGGGATAA CAACGTCTAT 
TTTTATTCCA TAGCATTTGG AAGACGCCAA GACACACTGA TGGGACATGA CGATGCTGTC 
AGTAAGATCT GTTGGCATAA TGACAGGCTG TACTCTGCAT CTTGGGACTC TACTGTGAAG 
GTGTGGTCCG GCGTTCCTGC AGAAATGCCA GGCACAAAAA GACACCAGTT TGACTTACTG 
GCTGAGCTTG AGCATGATGT CAGTGTCAAT ACGATCAATT TAAATGCTGT GAGTACACTG 
CTAGTTTCTG GCACCAAGGA AGGCCTGGTG AATATTTGGG ACCTCACTAC AGCCACTCTA 
TTACACCAGA TTTCATGCCA CTCAGGGACA GTATGTGATG CTGCTTTTAG CCCAGACAGT 
CGCCACGTCC TCAGCACAGG AGTAGACGGC TGCCTGAACG TGATTGATGT GCAGACGGGC 
ATGCTCATTT CCTCCATGGC CTCAGAGGAG CCGCAGAGGT GCTTTGTCTG GGATGGAAAC 
TCTGTCTTAT CTGGAAGTCG GACTGGTGAG CTGCTTGTCT GGGACCTCCT TGGAGCAAAA 
GTCAGTGAGC GGATACAGGG CCACACAGGT GCCGTGACGT GCATATGGAT GAATGAACAG 
TGTAGCAGCA TCATCACCGG AGGGGAAGAC AGACAAGTTA TGTTCTGGAA ACTGCAGTAT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nsmaf ( XM_008763545.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008761767.1 Rattus norvegicus neutral sphingomyelinase activation associated factor (Nsmaf), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa47649
Clone ID Related Accession (Same CDS sequence)	XM_008763545.2
Accession Version	XM_008763545.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2586bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	protein FAN isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008763545.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTTCA CCCGGAAGAG GCAGCGCGAG CAGCAGCTGC AGCTCTACTC CAAGGAGAGG 
TTCTCCTTAC TGCTGCTCAA CTTGGAGGAG TACTACTTCG AGCAGCATAC GGCTTTCCAT 
GTCCAGCACC AGGGCAGCCC CGAGGAAAGG AAAATCAGAG GCTCCTTAAA GATATGTTCA 
AAATCAGTGA TTTTTGAACC AGATGCAATA TCGCAGCCCA TTCTTAAGAT TCCTTTGAGA 
GACTGTTTAA AAATAGGAAA ACATGGAGAA AATGGAGCCA ATAAACACTT TGCAAAGGCA 
AAATCTTTGG GTATTTCACT CATTTTCAGT CAGGTATATT TCATTAAGGA GCACAACATC 
GTGGCACCGT ATAAGATAGA ACGGGGAAAA ATGGAGTATG TCTTCCAACT GGAAGTTTCT 
GGGAAGGTGG AAGATGTTGT GGAGACATTG CTACAGCTGC ACAGAGCATC CTGCCTTGAT 
AAACTTGGTG ACCAAATGGC CATGATAACA GCTATCTTGC AGTCACGTTT GGCTAGGACA 
TCATTTGACA AAAACAGGTT CCAAAGCGTT TCAGAAAAGC TGCACATGGA GTGCAAAGCA 
GAGATGGTGA CGCCTCTGGT GACGAACCCT GGGCATGTGT GCATTACTGA CACCAACCTG 
TATTTCCAGC CTCTCAACGG GTACCCGAAA CCTGTGGTCC AGATAACGCT CCAAGATGTC 
CGGCGCATTT ACAAAAGGAG GCACGGCCTC ATGCCTTTGG GCTTGGAAGT GTTTTGCACA 
GAAGATGACC TGTGTTCTGA CATATACCTA AAGTTCTATG AGCCTCAAGA CAGAGATGAC 
CTCTATTTTT ATATTGCCAC GTACCTAGAT TTGTCAAATC CTGCAACCTT CCGAGATCTT 
AGTAAGCCAG TGGGAGCCCT AAATCCAGAG AGGCTGGAGA GACTGCTGAC TCGATACCAG 
GAAATGCCTG AGCCGAAGTT CATGTATGGG AGCCACTACT CTTCCCCAGG CTATGTGCTC 
TTTTACCTCG TTAGGATCGC ACCAGAATAT ATGCTGTGTC TACAAAATGG AAGATTTGAT 
AATGCAGATA GGATGTTCAA CAGCATTGCA GAAACCTGGA AAAACTGTTT GGATGGTGCG 
ACGGACTTCA AAGAGTTGAT CCCAGAATTC TATGATGAAG ACGCCAGCTT CTTGATCAAT 
AGTCTGAAGT TGGATCTGGG GAAGAGACAA GGAGGGCAGA TGGTTGACGA CGTGGAGCTT 
CCTGCATGGG CCTCAAGTCC CCAAGACTTT CTCCAGAAGA ACAAGGACGC CTTGGAAAGC 
AGTTATGTGT CTGAGCACCT TCATGAGTGG ATTGATCTGA TATTTGGCTA TAAGCAAAAA 
GGGAGTGAGG CCATAGGGGC CCATAACGTG TTTCACCCCC TGACCTATGA AGGCGGTGTA 
GACCTGAACA GCATTGAGGA TCCCGATGAC AAAGTAGCCA TGCTGACCCA AATTTTGGAA 
TTTGGGCAGA CACCAAAACA GCTGTTTGTG ACACCACATC CTCGAAGGAT CACCCCCAAG 
TTTAAAAGTT TGTCCCAAGC GTCCAGCTAC AATGCTTCTA TGACAGACTC CCCAGTTTCT 
CCAGGGGAGG AGTCATTTGA AGACCTGACT GAAGAGAGCA AAACTCTGGC CTGGAATAAC 
ATCACCAAGT TGCAGCTACA TGAGCAGTAC AAAATCCACA AAGAGGCGGT TACAGGGATA 
GCAGTGTCTT GCAATGGGTC TTCAGTCTTT ACCACCTCAC AAGATTCCAC TTTGAAGATG 
TTTTCTAAGG AATCCAAAAT GTTACAAAGA AGTATATCAT TTTCAAATAT GGCTTTATCG 
TCTTGTTTAC TTTTGCCAGG AGACACCACT GTCATAAGTT CTTCTTGGGA TAACAACGTC 
TATTTTTATT CCATAGCATT TGGAAGACGC CAAGACACAC TGATGGGACA TGACGATGCT 
GTCAGTAAGA TCTGTTGGCA TAATGACAGG CTGTACTCTG CATCTTGGGA CTCTACTGTG 
AAGGTGTGGT CCGGCGTTCC TGCAGAAATG CCAGGCACAA AAAGACACCA GTTTGACTTA 
CTGGCTGAGC TTGAGCATGA TGTCAGTGTC AATACGATCA ATTTAAATGC TGTGAGTACA 
CTGCTAGTTT CTGGCACCAA GGAAGGCCTG GTGAATATTT GGGACCTCAC TACAGCCACT 
CTATTACACC AGATTTCATG CCACTCAGGG ACAGTATGTG ATGCTGCTTT TAGCCCAGAC 
AGTCGCCACG TCCTCAGCAC AGGAGTAGAC GGCTGCCTGA ACGTGATTGA TGTGCAGACG 
GGCATGCTCA TTTCCTCCAT GGCCTCAGAG GAGCCGCAGA GGTGCTTTGT CTGGGATGGA 
AACTCTGTCT TATCTGGAAG TCGGACTGGT GAGCTGCTTG TCTGGGACCT CCTTGGAGCA 
AAAGTCAGTG AGCGGATACA GGGCCACACA GGTGCCGTGA CGTGCATATG GATGAATGAA 
CAGTGTAGCA GCATCATCAC CGGAGGGGAA GACAGACAAG TTATGTTCTG GAAACTGCAG 
TATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008761767.1
CDS	1071..3656
Translation	MAFTRKRQREQQLQLYSKERFSLLLLNLEEYYFEQHTAFHVQHQGSPEERKIRGSLKICSKSVIFEPDAISQPILKIPLRDCLKIGKHGENGANKHFAKAKSLGISLIFSQVYFIKEHNIVAPYKIERGKMEYVFQLEVSGKVEDVVETLLQLHRASCLDKLGDQMAMITAILQSRLARTSFDKNRFQSVSEKLHMECKAEMVTPLVTNPGHVCITDTNLYFQPLNGYPKPVVQITLQDVRRIYKRRHGLMPLGLEVFCTEDDLCSDIYLKFYEPQDRDDLYFYIATYLDLSNPATFRDLSKPVGALNPERLERLLTRYQEMPEPKFMYGSHYSSPGYVLFYLVRIAPEYMLCLQNGRFDNADRMFNSIAETWKNCLDGATDFKELIPEFYDEDASFLINSLKLDLGKRQGGQMVDDVELPAWASSPQDFLQKNKDALESSYVSEHLHEWIDLIFGYKQKGSEAIGAHNVFHPLTYEGGVDLNSIEDPDDKVAMLTQILEFGQTPKQLFVTPHPRRITPKFKSLSQASSYNASMTDSPVSPGEESFEDLTEESKTLAWNNITKLQLHEQYKIHKEAVTGIAVSCNGSSVFTTSQDSTLKMFSKESKMLQRSISFSNMALSSCLLLPGDTTVISSSWDNNVYFYSIAFGRRQDTLMGHDDAVSKICWHNDRLYSASWDSTVKVWSGVPAEMPGTKRHQFDLLAELEHDVSVNTINLNAVSTLLVSGTKEGLVNIWDLTTATLLHQISCHSGTVCDAAFSPDSRHVLSTGVDGCLNVIDVQTGMLISSMASEEPQRCFVWDGNSVLSGSRTGELLVWDLLGAKVSERIQGHTGAVTCIWMNEQCSSIITGGEDRQVMFWKLQY

Target ORF information:

RefSeq Version	XM_008763545.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus neutral sphingomyelinase activation associated factor (Nsmaf), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008763545.2

ORF Insert Sequence:

ATGGCGTTCA CCCGGAAGAG GCAGCGCGAG CAGCAGCTGC AGCTCTACTC CAAGGAGAGG 
TTCTCCTTAC TGCTGCTCAA CTTGGAGGAG TACTACTTCG AGCAGCATAC GGCTTTCCAT 
GTCCAGCACC AGGGCAGCCC CGAGGAAAGG AAAATCAGAG GCTCCTTAAA GATATGTTCA 
AAATCAGTGA TTTTTGAACC AGATGCAATA TCGCAGCCCA TTCTTAAGAT TCCTTTGAGA 
GACTGTTTAA AAATAGGAAA ACATGGAGAA AATGGAGCCA ATAAACACTT TGCAAAGGCA 
AAATCTTTGG GTATTTCACT CATTTTCAGT CAGGTATATT TCATTAAGGA GCACAACATC 
GTGGCACCGT ATAAGATAGA ACGGGGAAAA ATGGAGTATG TCTTCCAACT GGAAGTTTCT 
GGGAAGGTGG AAGATGTTGT GGAGACATTG CTACAGCTGC ACAGAGCATC CTGCCTTGAT 
AAACTTGGTG ACCAAATGGC CATGATAACA GCTATCTTGC AGTCACGTTT GGCTAGGACA 
TCATTTGACA AAAACAGGTT CCAAAGCGTT TCAGAAAAGC TGCACATGGA GTGCAAAGCA 
GAGATGGTGA CGCCTCTGGT GACGAACCCT GGGCATGTGT GCATTACTGA CACCAACCTG 
TATTTCCAGC CTCTCAACGG GTACCCGAAA CCTGTGGTCC AGATAACGCT CCAAGATGTC 
CGGCGCATTT ACAAAAGGAG GCACGGCCTC ATGCCTTTGG GCTTGGAAGT GTTTTGCACA 
GAAGATGACC TGTGTTCTGA CATATACCTA AAGTTCTATG AGCCTCAAGA CAGAGATGAC 
CTCTATTTTT ATATTGCCAC GTACCTAGAT TTGTCAAATC CTGCAACCTT CCGAGATCTT 
AGTAAGCCAG TGGGAGCCCT AAATCCAGAG AGGCTGGAGA GACTGCTGAC TCGATACCAG 
GAAATGCCTG AGCCGAAGTT CATGTATGGG AGCCACTACT CTTCCCCAGG CTATGTGCTC 
TTTTACCTCG TTAGGATCGC ACCAGAATAT ATGCTGTGTC TACAAAATGG AAGATTTGAT 
AATGCAGATA GGATGTTCAA CAGCATTGCA GAAACCTGGA AAAACTGTTT GGATGGTGCG 
ACGGACTTCA AAGAGTTGAT CCCAGAATTC TATGATGAAG ACGCCAGCTT CTTGATCAAT 
AGTCTGAAGT TGGATCTGGG GAAGAGACAA GGAGGGCAGA TGGTTGACGA CGTGGAGCTT 
CCTGCATGGG CCTCAAGTCC CCAAGACTTT CTCCAGAAGA ACAAGGACGC CTTGGAAAGC 
AGTTATGTGT CTGAGCACCT TCATGAGTGG ATTGATCTGA TATTTGGCTA TAAGCAAAAA 
GGGAGTGAGG CCATAGGGGC CCATAACGTG TTTCACCCCC TGACCTATGA AGGCGGTGTA 
GACCTGAACA GCATTGAGGA TCCCGATGAC AAAGTAGCCA TGCTGACCCA AATTTTGGAA 
TTTGGGCAGA CACCAAAACA GCTGTTTGTG ACACCACATC CTCGAAGGAT CACCCCCAAG 
TTTAAAAGTT TGTCCCAAGC GTCCAGCTAC AATGCTTCTA TGACAGACTC CCCAGTTTCT 
CCAGGGGAGG AGTCATTTGA AGACCTGACT GAAGAGAGCA AAACTCTGGC CTGGAATAAC 
ATCACCAAGT TGCAGCTACA TGAGCAGTAC AAAATCCACA AAGAGGCGGT TACAGGGATA 
GCAGTGTCTT GCAATGGGTC TTCAGTCTTT ACCACCTCAC AAGATTCCAC TTTGAAGATG 
TTTTCTAAGG AATCCAAAAT GTTACAAAGA AGTATATCAT TTTCAAATAT GGCTTTATCG 
TCTTGTTTAC TTTTGCCAGG AGACACCACT GTCATAAGTT CTTCTTGGGA TAACAACGTC 
TATTTTTATT CCATAGCATT TGGAAGACGC CAAGACACAC TGATGGGACA TGACGATGCT 
GTCAGTAAGA TCTGTTGGCA TAATGACAGG CTGTACTCTG CATCTTGGGA CTCTACTGTG 
AAGGTGTGGT CCGGCGTTCC TGCAGAAATG CCAGGCACAA AAAGACACCA GTTTGACTTA 
CTGGCTGAGC TTGAGCATGA TGTCAGTGTC AATACGATCA ATTTAAATGC TGTGAGTACA 
CTGCTAGTTT CTGGCACCAA GGAAGGCCTG GTGAATATTT GGGACCTCAC TACAGCCACT 
CTATTACACC AGATTTCATG CCACTCAGGG ACAGTATGTG ATGCTGCTTT TAGCCCAGAC 
AGTCGCCACG TCCTCAGCAC AGGAGTAGAC GGCTGCCTGA ACGTGATTGA TGTGCAGACG 
GGCATGCTCA TTTCCTCCAT GGCCTCAGAG GAGCCGCAGA GGTGCTTTGT CTGGGATGGA 
AACTCTGTCT TATCTGGAAG TCGGACTGGT GAGCTGCTTG TCTGGGACCT CCTTGGAGCA 
AAAGTCAGTG AGCGGATACA GGGCCACACA GGTGCCGTGA CGTGCATATG GATGAATGAA 
CAGTGTAGCA GCATCATCAC CGGAGGGGAA GACAGACAAG TTATGTTCTG GAAACTGCAG 
TATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nsmaf ( XM_017593446.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017448935.1 Rattus norvegicus neutral sphingomyelinase activation associated factor (Nsmaf), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa61268
Clone ID Related Accession (Same CDS sequence)	XM_017593446.1
Accession Version	XM_017593446.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2328bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	protein FAN isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGTTCA CCCGGAAGAG GCAGCGCGAG CAGCAGCTGC AGCTCTACTC CAAGGAGAGG 
TTCTCCTTAC TGCTGCTCAA CTTGGAGGAG TACTACTTCG AGCAGCATAC GGCTTTCCAT 
GTCCAGCACC AGGGCAGCCC CGAGGAAAGG AAAATCAGAG GCTCCTTAAA GATATGTTCA 
AAATCAGTGA TTTTTGAACC AGATGCAATA TCGCAGCCCA TTCTTAAGAT TCCTTTGAGA 
GACTGTTTAA AAATAGGAAA ACATGGAGAA AATGGAGCCA ATAAACACTT TGCAAAGGCA 
AAATCTTTGG GTATTTCACT CATTTTCAGT CAGGTATATT TCATTAAGGA GCACAACATC 
GTGGCACCGT ATAAGATAGA ACGGGGAAAA ATGGAGTATG TCTTCCAACT GGAAGTTTCT 
GGGAAGGTGG AAGATGTTGT GGAGACATTG CTACAGCTGC ACAGAGCATC CTGCCTTGAT 
AAACTTGGTG ACCAAATGGC CATGATAACA GCTATCTTGC AGTCACGTTT GGCTAGGACA 
TCATTTGACA AAAACAGGTT CCAAAGCGTT TCAGAAAAGC TGCACATGGA GTGCAAAGCA 
GAGATGGTGA CGCCTCTGGT GACGAACCCT GGGCATGTGT GCATTACTGA CACCAACCTG 
TATTTCCAGC CTCTCAACGG GTACCCGAAA CCTGTGGTCC AGATAACGCT CCAAGATGTC 
CGGCGCATTT ACAAAAGGAG GCACGGCCTC ATGCCTTTGG GCTTGGAAGT GTTTTGCACA 
GAAGATGACC TGTGTTCTGA CATATACCTA AAGTTCTATG AGCCTCAAGA CAGAGATGAC 
CTCTATTTTT ATATTGCCAC GTACCTAGAA CACCACGTGG CAGAACACAC TGCGGAGAGC 
TACATGCTGC AGTGGCAGCG AGGGCACCTG TCCAACTACC AGTACCTGCT CCACCTCAAC 
AACCTGGCCG ACCGCAGCTG CAATGACCTG TCCCAGTACC CTGTGTTTCC ATGGGTAATA 
AGTGACTACT CCAGCCCAGA GCTAGATTTG TCAAATCCTG CAACCTTCCG AGATCTTAGT 
AAGCCAGTGG GAGCCCTAAA TCCAGAGAGG CTGGAGAGAC TGCTGACTCG ATACCAGGAA 
ATGCCTGAGC CGAAGTTCAT GTATGGGAGC CACTACTCTT CCCCAGGCTA TGTGCTCTTT 
TACCTCGTTA GGATCGCACC AGAATATATG CTGTGTCTAC AAAATGGAAG ATTTGATAAT 
GCAGATAGGA TGTTCAACAG CATTGCAGAA ACCTGGAAAA ACTGTTTGGA TGGTGCGACG 
GACTTCAAAG AGTTGATCCC AGAATTCTAT GATGAAGACG CCAGCTTCTT GATCAATAGT 
CTGAAGTTGG ATCTGGGGAA GAGACAAGGA GGGCAGATGG TTGACGACGT GGAGCTTCCT 
GCATGGGCCT CAAGTCCCCA AGACTTTCTC CAGAAGAACA AGGACGCCTT GGAAAGCAGT 
TATGTGTCTG AGCACCTTCA TGAGTGGATT GATCTGATAT TTGGCTATAA GCAAAAAGGG 
AGTGAGGCCA TAGGGGCCCA TAACGTGTTT CACCCCCTGA CCTATGAAGG CGGTGTAGAC 
CTGAACAGCA TTGAGGATCC CGATGACAAA GTAGCCATGC TGACCCAAAT TTTGGAATTT 
GGGCAGACAC CAAAACAGCT GTTTGTGACA CCACATCCTC GAAGGATCAC CCCCAAGTTT 
AAAAGTTTGT CCCAAGCGTC CAGCTACAAT GCTTCTATGA CAGACTCCCC AGTTTCTCCA 
GGGGAGGAGT CATTTGAAGA CCTGACTGAA GAGAGCAAAA CTCTGGCCTG GAATAACATC 
ACCAAGTTGC AGCTACATGA GCAGTACAAA ATCCACAAAG AGGCGGTTAC AGGGATAGCA 
GTGTCTTGCA ATGGGTCTTC AGTCTTTACC ACCTCACAAG ATTCCACTTT GAAGATGTTT 
TCTAAGGAAT CCAAAATGTT ACAAAGAAGT ATATCATTTT CAAATATGGC TTTATCGTCT 
TGTTTACTTT TGCCAGGAGA CACCACTGTC ATAAGTTCTT CTTGGGATAA CAACGTCTAT 
TTTTATTCCA TAGCATTTGG AAGACGCCAA GACACACTGA TGGGACATGA CGATGCTGTC 
AGTAAGATCT GTTGGCATAA TGACAGGCTG TACTCTGCAT CTTGGGACTC TACTGTGAAG 
GTGTGGTCCG GCGTTCCTGC AGAAATGCCA GGCACAAAAA GACACCAGTT TGACTTACTG 
GCTGAGCTTG AGCATGATGT CAGTGTGAGT CCAACAGCAG AGCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017448935.1
CDS	1072..3399
Translation	MAFTRKRQREQQLQLYSKERFSLLLLNLEEYYFEQHTAFHVQHQGSPEERKIRGSLKICSKSVIFEPDAISQPILKIPLRDCLKIGKHGENGANKHFAKAKSLGISLIFSQVYFIKEHNIVAPYKIERGKMEYVFQLEVSGKVEDVVETLLQLHRASCLDKLGDQMAMITAILQSRLARTSFDKNRFQSVSEKLHMECKAEMVTPLVTNPGHVCITDTNLYFQPLNGYPKPVVQITLQDVRRIYKRRHGLMPLGLEVFCTEDDLCSDIYLKFYEPQDRDDLYFYIATYLEHHVAEHTAESYMLQWQRGHLSNYQYLLHLNNLADRSCNDLSQYPVFPWVISDYSSPELDLSNPATFRDLSKPVGALNPERLERLLTRYQEMPEPKFMYGSHYSSPGYVLFYLVRIAPEYMLCLQNGRFDNADRMFNSIAETWKNCLDGATDFKELIPEFYDEDASFLINSLKLDLGKRQGGQMVDDVELPAWASSPQDFLQKNKDALESSYVSEHLHEWIDLIFGYKQKGSEAIGAHNVFHPLTYEGGVDLNSIEDPDDKVAMLTQILEFGQTPKQLFVTPHPRRITPKFKSLSQASSYNASMTDSPVSPGEESFEDLTEESKTLAWNNITKLQLHEQYKIHKEAVTGIAVSCNGSSVFTTSQDSTLKMFSKESKMLQRSISFSNMALSSCLLLPGDTTVISSSWDNNVYFYSIAFGRRQDTLMGHDDAVSKICWHNDRLYSASWDSTVKVWSGVPAEMPGTKRHQFDLLAELEHDVSVSPTAEP

Target ORF information:

RefSeq Version	XM_017593446.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus neutral sphingomyelinase activation associated factor (Nsmaf), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017593446.1

ORF Insert Sequence:

ATGGCGTTCA CCCGGAAGAG GCAGCGCGAG CAGCAGCTGC AGCTCTACTC CAAGGAGAGG 
TTCTCCTTAC TGCTGCTCAA CTTGGAGGAG TACTACTTCG AGCAGCATAC GGCTTTCCAT 
GTCCAGCACC AGGGCAGCCC CGAGGAAAGG AAAATCAGAG GCTCCTTAAA GATATGTTCA 
AAATCAGTGA TTTTTGAACC AGATGCAATA TCGCAGCCCA TTCTTAAGAT TCCTTTGAGA 
GACTGTTTAA AAATAGGAAA ACATGGAGAA AATGGAGCCA ATAAACACTT TGCAAAGGCA 
AAATCTTTGG GTATTTCACT CATTTTCAGT CAGGTATATT TCATTAAGGA GCACAACATC 
GTGGCACCGT ATAAGATAGA ACGGGGAAAA ATGGAGTATG TCTTCCAACT GGAAGTTTCT 
GGGAAGGTGG AAGATGTTGT GGAGACATTG CTACAGCTGC ACAGAGCATC CTGCCTTGAT 
AAACTTGGTG ACCAAATGGC CATGATAACA GCTATCTTGC AGTCACGTTT GGCTAGGACA 
TCATTTGACA AAAACAGGTT CCAAAGCGTT TCAGAAAAGC TGCACATGGA GTGCAAAGCA 
GAGATGGTGA CGCCTCTGGT GACGAACCCT GGGCATGTGT GCATTACTGA CACCAACCTG 
TATTTCCAGC CTCTCAACGG GTACCCGAAA CCTGTGGTCC AGATAACGCT CCAAGATGTC 
CGGCGCATTT ACAAAAGGAG GCACGGCCTC ATGCCTTTGG GCTTGGAAGT GTTTTGCACA 
GAAGATGACC TGTGTTCTGA CATATACCTA AAGTTCTATG AGCCTCAAGA CAGAGATGAC 
CTCTATTTTT ATATTGCCAC GTACCTAGAA CACCACGTGG CAGAACACAC TGCGGAGAGC 
TACATGCTGC AGTGGCAGCG AGGGCACCTG TCCAACTACC AGTACCTGCT CCACCTCAAC 
AACCTGGCCG ACCGCAGCTG CAATGACCTG TCCCAGTACC CTGTGTTTCC ATGGGTAATA 
AGTGACTACT CCAGCCCAGA GCTAGATTTG TCAAATCCTG CAACCTTCCG AGATCTTAGT 
AAGCCAGTGG GAGCCCTAAA TCCAGAGAGG CTGGAGAGAC TGCTGACTCG ATACCAGGAA 
ATGCCTGAGC CGAAGTTCAT GTATGGGAGC CACTACTCTT CCCCAGGCTA TGTGCTCTTT 
TACCTCGTTA GGATCGCACC AGAATATATG CTGTGTCTAC AAAATGGAAG ATTTGATAAT 
GCAGATAGGA TGTTCAACAG CATTGCAGAA ACCTGGAAAA ACTGTTTGGA TGGTGCGACG 
GACTTCAAAG AGTTGATCCC AGAATTCTAT GATGAAGACG CCAGCTTCTT GATCAATAGT 
CTGAAGTTGG ATCTGGGGAA GAGACAAGGA GGGCAGATGG TTGACGACGT GGAGCTTCCT 
GCATGGGCCT CAAGTCCCCA AGACTTTCTC CAGAAGAACA AGGACGCCTT GGAAAGCAGT 
TATGTGTCTG AGCACCTTCA TGAGTGGATT GATCTGATAT TTGGCTATAA GCAAAAAGGG 
AGTGAGGCCA TAGGGGCCCA TAACGTGTTT CACCCCCTGA CCTATGAAGG CGGTGTAGAC 
CTGAACAGCA TTGAGGATCC CGATGACAAA GTAGCCATGC TGACCCAAAT TTTGGAATTT 
GGGCAGACAC CAAAACAGCT GTTTGTGACA CCACATCCTC GAAGGATCAC CCCCAAGTTT 
AAAAGTTTGT CCCAAGCGTC CAGCTACAAT GCTTCTATGA CAGACTCCCC AGTTTCTCCA 
GGGGAGGAGT CATTTGAAGA CCTGACTGAA GAGAGCAAAA CTCTGGCCTG GAATAACATC 
ACCAAGTTGC AGCTACATGA GCAGTACAAA ATCCACAAAG AGGCGGTTAC AGGGATAGCA 
GTGTCTTGCA ATGGGTCTTC AGTCTTTACC ACCTCACAAG ATTCCACTTT GAAGATGTTT 
TCTAAGGAAT CCAAAATGTT ACAAAGAAGT ATATCATTTT CAAATATGGC TTTATCGTCT 
TGTTTACTTT TGCCAGGAGA CACCACTGTC ATAAGTTCTT CTTGGGATAA CAACGTCTAT 
TTTTATTCCA TAGCATTTGG AAGACGCCAA GACACACTGA TGGGACATGA CGATGCTGTC 
AGTAAGATCT GTTGGCATAA TGACAGGCTG TACTCTGCAT CTTGGGACTC TACTGTGAAG 
GTGTGGTCCG GCGTTCCTGC AGAAATGCCA GGCACAAAAA GACACCAGTT TGACTTACTG 
GCTGAGCTTG AGCATGATGT CAGTGTGAGT CCAACAGCAG AGCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Neutral sphingomyelinase, NADPH oxidase and reactive oxygen species. Role in acute hypoxic pulmonary vasoconstriction.
Journal of cellular physiology226(10)2633-40(2011 Oct)
Frazziano G,Moreno L,Moral-Sanz J,Menendez C,Escolano L,Gonzalez C,Villamor E,Alvarez-Sala JL,Cogolludo AL,Perez-Vizcaino F

The group VIA calcium-independent phospholipase A2 participates in ER stress-induced INS-1 insulinoma cell apoptosis by promoting ceramide generation via hydrolysis of sphingomyelins by neutral sphingomyelinase.
Biochemistry46(35)10170-85(2007 Sep)
Lei X,Zhang S,Bohrer A,Bao S,Song H,Ramanadham S

Factor associated with neutral sphingomyelinase activation and its role in cardiac cell death.
Circulation research92(6)589-91(2003 Apr)
O'Brien NW,Gellings NM,Guo M,Barlow SB,Glembotski CC,Sabbadini RA

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Nsmaf cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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