Nsmaf cDNA ORF clone, Mus musculus(house mouse)

The following Nsmaf gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nsmaf cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu88461 XM_030253309.1
Latest version!
Mus musculus neutral sphingomyelinase (N-SMase) activation associated factor (Nsmaf), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OMu07211 NM_010945.2
Latest version!
Mus musculus neutral sphingomyelinase (N-SMase) activation associated factor (Nsmaf), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $545.30
$779.00
OMu32752 XM_006537662.4
Latest version!
Mus musculus neutral sphingomyelinase (N-SMase) activation associated factor (Nsmaf), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OMu32753 XM_006537663.3
Latest version!
Mus musculus neutral sphingomyelinase (N-SMase) activation associated factor (Nsmaf), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OMu32754 XM_006537664.3
Latest version!
Mus musculus neutral sphingomyelinase (N-SMase) activation associated factor (Nsmaf), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OMu68335 XM_011249943.3
Latest version!
Mus musculus neutral sphingomyelinase (N-SMase) activation associated factor (Nsmaf), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OMu68336 XM_011249944.3
Latest version!
Mus musculus neutral sphingomyelinase (N-SMase) activation associated factor (Nsmaf), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
View Old Accession Versions >>
OMu32752 XM_006537662.3 Mus musculus neutral sphingomyelinase (N-SMase) activation associated factor (Nsmaf), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OMu32753 XM_006537663.2 Mus musculus neutral sphingomyelinase (N-SMase) activation associated factor (Nsmaf), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OMu32754 XM_006537664.2 Mus musculus neutral sphingomyelinase (N-SMase) activation associated factor (Nsmaf), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OMu68335 XM_011249943.2 Mus musculus neutral sphingomyelinase (N-SMase) activation associated factor (Nsmaf), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
OMu68336 XM_011249944.2 Mus musculus neutral sphingomyelinase (N-SMase) activation associated factor (Nsmaf), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu88461
    Clone ID Related Accession (Same CDS sequence) XM_030253309.1
    Accession Version XM_030253309.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1452bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product protein FAN isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGCGTTCA CCCGGAAGAG GCAGCGCGAG CAGCAGCTGC AGCTCTACTC CAAGGAGAGA 
    TGCGGGTTTG TCTGTGGGAG CCGGGATCCG ACGGTCACGT TGTTGCTGAG TGGATCCAGC
    GAAGCAGGCT ATGCCAGGAA ATGCAGGTTT TCCTTACTGC TTCTCAACTT GGAGGAGTAC
    TACTTTGAGC AGCACACAGC GTTCCATGTC CAGCACCAGG GCAGCCAGGA AGAAAGGAAA
    ATCAGAGGCT CCTTAAAGAT ATGTTCAAAA TCAGTGATTT TTGAACCAGA TGCAATATCT
    CAGCCCATTC TTAAGATTCC TTTGAGAGAC TGTTTAAAAA TAGGAAAACA TGGAGAAAAT
    GGAGCCAATA AACACTTTGC AAAGGCAAAA TCATGGGGTA TTTCACTCAT TTTCAGTCAG
    ATATACTTTA TTAAGGAGCA CAACATTGTG GCACCGTATA AGATAGAGCG GGGAAAAATG
    GAGTATGTCT TTGAACTGGA AGTTTCTGGG AAGGTGGAAG ATGTTGTGGA GACATTGCTA
    CAGCTGCACA GAGCATCCTG CCTTGATAAA CTTGGTGACC AAATGGCCAT GATAACAGCT
    ATCTTGCAGT CACGTCTGGC TAGGACATCA TTTGACAAAA ACAGGTTCCA AAGTGTTTCT
    GAAAAGCTGC ACATGGAGTG CAAAGCAGAG ATGGTAACAC CTCTAGTGAC GAACCCTGGG
    CATGTGTGCA TTACAGACAC CAGCCTGTAT TTCCAGCCTC TCAATGGGTA CCCGAAACCT
    GTGGTCCAGA TAACACTGCA GGATGTCCGG CGCATTTACA AAAGGAGGCA CGGCCTCATG
    CCTTTGGGCT TGGAAGTGTT TTGCACAGAT GACGACCTGT GTTCCGACAT ATACCTAAAG
    TTCTATGAGC CTCAAGACAG AGATGATCTC TATTTTTATA TCGCCACGTA CCTAGAACAC
    CACGCTGCAG AACACACTGC GGAGAGCTAC ATGCTGCAGT GGCAGCGAGG GCACCTTTCC
    AACTACCAGT ACCTGCTCCA CCTCAACAAC CTGGCCGACC GCAGCTGCAA TGACCTGTCC
    CAGTACCCTG TGTTTCCATG GATAATAAGT GATTACTCCA GCCCAGAGCT AGATTTGTCA
    AATCCTGCAA CCTTCCGAGA TCTTAGTAAG CCAGTGGGAG CCCTAAATGC GGAGCGACTG
    GAGAGACTGC TGACTCGATA CCAGGAAATG CCTGAGCCAA GATTCATGTA TGGGAGTCAC
    TACTCTTCTC CAGGCTATGT GCTCTTTTAC CTCGTTAGGA TCGCACCAGA GTATATGCTG
    TGTCTACAAA ATGGAAGATT TGATAATGCA GATAGGATGT TTAACAGGAG CGAGAAGATT
    TTTCTGGAAC TGGATTTACA GACAGTTATG GGCCACCATG TAGGTGCTAG GAATCCAACC
    CACGTCTTCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030109169.1
    CDS798..2249
    Translation

    Target ORF information:

    RefSeq Version XM_030253309.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus neutral sphingomyelinase (N-SMase) activation associated factor (Nsmaf), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030253309.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGCGTTCA CCCGGAAGAG GCAGCGCGAG CAGCAGCTGC AGCTCTACTC CAAGGAGAGA 
    TGCGGGTTTG TCTGTGGGAG CCGGGATCCG ACGGTCACGT TGTTGCTGAG TGGATCCAGC
    GAAGCAGGCT ATGCCAGGAA ATGCAGGTTT TCCTTACTGC TTCTCAACTT GGAGGAGTAC
    TACTTTGAGC AGCACACAGC GTTCCATGTC CAGCACCAGG GCAGCCAGGA AGAAAGGAAA
    ATCAGAGGCT CCTTAAAGAT ATGTTCAAAA TCAGTGATTT TTGAACCAGA TGCAATATCT
    CAGCCCATTC TTAAGATTCC TTTGAGAGAC TGTTTAAAAA TAGGAAAACA TGGAGAAAAT
    GGAGCCAATA AACACTTTGC AAAGGCAAAA TCATGGGGTA TTTCACTCAT TTTCAGTCAG
    ATATACTTTA TTAAGGAGCA CAACATTGTG GCACCGTATA AGATAGAGCG GGGAAAAATG
    GAGTATGTCT TTGAACTGGA AGTTTCTGGG AAGGTGGAAG ATGTTGTGGA GACATTGCTA
    CAGCTGCACA GAGCATCCTG CCTTGATAAA CTTGGTGACC AAATGGCCAT GATAACAGCT
    ATCTTGCAGT CACGTCTGGC TAGGACATCA TTTGACAAAA ACAGGTTCCA AAGTGTTTCT
    GAAAAGCTGC ACATGGAGTG CAAAGCAGAG ATGGTAACAC CTCTAGTGAC GAACCCTGGG
    CATGTGTGCA TTACAGACAC CAGCCTGTAT TTCCAGCCTC TCAATGGGTA CCCGAAACCT
    GTGGTCCAGA TAACACTGCA GGATGTCCGG CGCATTTACA AAAGGAGGCA CGGCCTCATG
    CCTTTGGGCT TGGAAGTGTT TTGCACAGAT GACGACCTGT GTTCCGACAT ATACCTAAAG
    TTCTATGAGC CTCAAGACAG AGATGATCTC TATTTTTATA TCGCCACGTA CCTAGAACAC
    CACGCTGCAG AACACACTGC GGAGAGCTAC ATGCTGCAGT GGCAGCGAGG GCACCTTTCC
    AACTACCAGT ACCTGCTCCA CCTCAACAAC CTGGCCGACC GCAGCTGCAA TGACCTGTCC
    CAGTACCCTG TGTTTCCATG GATAATAAGT GATTACTCCA GCCCAGAGCT AGATTTGTCA
    AATCCTGCAA CCTTCCGAGA TCTTAGTAAG CCAGTGGGAG CCCTAAATGC GGAGCGACTG
    GAGAGACTGC TGACTCGATA CCAGGAAATG CCTGAGCCAA GATTCATGTA TGGGAGTCAC
    TACTCTTCTC CAGGCTATGT GCTCTTTTAC CTCGTTAGGA TCGCACCAGA GTATATGCTG
    TGTCTACAAA ATGGAAGATT TGATAATGCA GATAGGATGT TTAACAGGAG CGAGAAGATT
    TTTCTGGAAC TGGATTTACA GACAGTTATG GGCCACCATG TAGGTGCTAG GAATCCAACC
    CACGTCTTCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu07211
    Clone ID Related Accession (Same CDS sequence) NM_010945.2
    Accession Version NM_010945.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2763bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-26
    Organism Mus musculus(house mouse)
    Product protein FAN
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY729997.1, BC145963.1 and CO043098.1. On Apr 2, 2008 this sequence version replaced NM_010945.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF013632.1, BC145961.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164139 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCGTTCA CCCGGAAGAG GCAGCGCGAG CAGCAGCTGC AGCTCTACTC CAAGGAGAGG 
    TTTTCCTTAC TGCTTCTCAA CTTGGAGGAG TACTACTTTG AGCAGCACAC AGCGTTCCAT
    GTCCAGCACC AGGGCAGCCA GGAAGAAAGG AAAATCAGAG GCTCCTTAAA GATATGTTCA
    AAATCAGTGA TTTTTGAACC AGATGCAATA TCTCAGCCCA TTCTTAAGAT TCCTTTGAGA
    GACTGTTTAA AAATAGGAAA ACATGGAGAA AATGGAGCCA ATAAACACTT TGCAAAGGCA
    AAATCATGGG GTATTTCACT CATTTTCAGT CAGATATACT TTATTAAGGA GCACAACATT
    GTGGCACCGT ATAAGATAGA GCGGGGAAAA ATGGAGTATG TCTTTGAACT GGAAGTTTCT
    GGGAAGGTGG AAGATGTTGT GGAGACATTG CTACAGCTGC ACAGAGCATC CTGCCTTGAT
    AAACTTGGTG ACCAAATGGC CATGATAACA GCTATCTTGC AGTCACGTCT GGCTAGGACA
    TCATTTGACA AAAACAGGTT CCAAAGTGTT TCTGAAAAGC TGCACATGGA GTGCAAAGCA
    GAGATGGTAA CACCTCTAGT GACGAACCCT GGGCATGTGT GCATTACAGA CACCAGCCTG
    TATTTCCAGC CTCTCAATGG GTACCCGAAA CCTGTGGTCC AGATAACACT GCAGGATGTC
    CGGCGCATTT ACAAAAGGAG GCACGGCCTC ATGCCTTTGG GCTTGGAAGT GTTTTGCACA
    GATGACGACC TGTGTTCCGA CATATACCTA AAGTTCTATG AGCCTCAAGA CAGAGATGAT
    CTCTATTTTT ATATCGCCAC GTACCTAGAA CACCACGCTG CAGAACACAC TGCGGAGAGC
    TACATGCTGC AGTGGCAGCG AGGGCACCTT TCCAACTACC AGTACCTGCT CCACCTCAAC
    AACCTGGCCG ACCGCAGCTG CAATGACCTG TCCCAGTACC CTGTGTTTCC ATGGATAATA
    AGTGATTACT CCAGCCCAGA GCTAGATTTG TCAAATCCTG CAACCTTCCG AGATCTTAGT
    AAGCCAGTGG GAGCCCTAAA TGCGGAGCGA CTGGAGAGAC TGCTGACTCG ATACCAGGAA
    ATGCCTGAGC CAAGATTCAT GTATGGGAGT CACTACTCTT CTCCAGGCTA TGTGCTCTTT
    TACCTCGTTA GGATCGCACC AGAGTATATG CTGTGTCTAC AAAATGGAAG ATTTGATAAT
    GCAGATAGGA TGTTTAACAG TATTGCAGAA ACGTGGAAAA ACTGTTTGGA TGGTGCAACG
    GATTTTAAAG AGTTGATTCC AGAATTCTAT GATGAAGACG TCAGCTTTTT GGTCAATAGT
    CTGAAGCTGG ATCTGGGAAA GAGACAAGGC GGGCAGATGG TTGATGACGT GGACCTTCCA
    GCCTGGGCCT CAAGTCCCCA AGACTTCCTG CAGAAGAACA AGGATGCCTT GGAAAGCGGT
    TACGTGTCTG AGCACCTTCA TGAGTGGATT GATCTGATAT TTGGCTATAA GCAAAAAGGG
    AGTGAGGCCA TAGGGGCCCA TAACGTGTTT CATCCCCTGA CCTATGAAGG TGGTGTAGAC
    TTGAACAGCA TTGAGGATCC TGATGAAAAA GTAGCCATGC TGACCCAAAT TTTGGAATTT
    GGGCAGACAC CAAAACAGCT GTTTGTGACA CCACATCCTC GAAGGATCAC CCCCAAGTTT
    AAAAGTTTGT CCCAAGCCTC CAGCTACAAC GCTTCTCTGA CAGACTCCCC AGTTTCTCCA
    GGGGAGGAGT CGTTTGAAGA CCTGACTGAG GAAAGCAGAA CTCTGGCCTG GAGTAATATC
    GCCAAGCTGC AGCTACATGA GCAGTACAAG ATCCACAAAG AGGCGGTTAC AGGGATAGCA
    GTGTCTTGCA ATGGCTCTTC AGTCTTTACC ACCTCCCAAG ATTCCACTTT GAAGATGTTT
    TCTAAGGAAT CCAAAATGCT ACAAAGAAGT ATATCATTTT CCAATATGGC TTTATCCTCT
    TGTTTACTGT TGCCGGGAGA CACCACTGTC ATAAGTTCTT CTTGGGATAA CAACGTCTAT
    TTTTATTCCA TAGCATTTGG AAGACGCCAA GATACACTGA TGGGACATGA CGATGCTGTC
    AGTAAGATCT GTTGGCATAA CGACAGGCTG TACTCTGGAT CTTGGGACTC TACTGTGAAG
    GTGTGGTCCG GTGTTCCTGC AGAAATGCCA GGAACAAAAA GACACCAGTT TGACTTACTG
    GCTGAGCTTG AACATGATGT CAGCGTCAAT ACTATCAATT TAAATGCTGT GAGCACACTG
    TTAGTTTCTG GCACCAAAGA AGGCATGGTG AATATTTGGG ACCTCACTAC AGCCACTTTA
    TTACACCAGA CTTCATGCCA TTCAGGGACA GTATGTGATG CTGCTTTTAG CCCAGATAGT
    CGCCACATCC TCAGCACGGG CGTAGATGGA TGTCTCAATG TGATTGATGT GCAGACGGGC
    ATGCTCATCT CCTCCATGGC CTCAGAGGAG CCGCAGAGGT GCTTTGTCTG GGATGGAAAT
    TCTGTCTTAT CTGGAAGTCG GTCTGGTGAG CTGCTTGTTT GGGACCTCCT TGGAGCAAAA
    GTCAGTGAGC GGATTCAGGG CCACACTGGT GCTGTGACAT GCATGTGGAT GAACGAACAG
    TGTAGCAGTA TTATCACCGG AGGGGAAGAC AGACAAATTA TGTTCTGGAA ATTGCAGTAT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_035075.2
    CDS229..2991
    Translation

    Target ORF information:

    RefSeq Version NM_010945.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus neutral sphingomyelinase (N-SMase) activation associated factor (Nsmaf), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_010945.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCGTTCA CCCGGAAGAG GCAGCGCGAG CAGCAGCTGC AGCTCTACTC CAAGGAGAGG 
    TTTTCCTTAC TGCTTCTCAA CTTGGAGGAG TACTACTTTG AGCAGCACAC AGCGTTCCAT
    GTCCAGCACC AGGGCAGCCA GGAAGAAAGG AAAATCAGAG GCTCCTTAAA GATATGTTCA
    AAATCAGTGA TTTTTGAACC AGATGCAATA TCTCAGCCCA TTCTTAAGAT TCCTTTGAGA
    GACTGTTTAA AAATAGGAAA ACATGGAGAA AATGGAGCCA ATAAACACTT TGCAAAGGCA
    AAATCATGGG GTATTTCACT CATTTTCAGT CAGATATACT TTATTAAGGA GCACAACATT
    GTGGCACCGT ATAAGATAGA GCGGGGAAAA ATGGAGTATG TCTTTGAACT GGAAGTTTCT
    GGGAAGGTGG AAGATGTTGT GGAGACATTG CTACAGCTGC ACAGAGCATC CTGCCTTGAT
    AAACTTGGTG ACCAAATGGC CATGATAACA GCTATCTTGC AGTCACGTCT GGCTAGGACA
    TCATTTGACA AAAACAGGTT CCAAAGTGTT TCTGAAAAGC TGCACATGGA GTGCAAAGCA
    GAGATGGTAA CACCTCTAGT GACGAACCCT GGGCATGTGT GCATTACAGA CACCAGCCTG
    TATTTCCAGC CTCTCAATGG GTACCCGAAA CCTGTGGTCC AGATAACACT GCAGGATGTC
    CGGCGCATTT ACAAAAGGAG GCACGGCCTC ATGCCTTTGG GCTTGGAAGT GTTTTGCACA
    GATGACGACC TGTGTTCCGA CATATACCTA AAGTTCTATG AGCCTCAAGA CAGAGATGAT
    CTCTATTTTT ATATCGCCAC GTACCTAGAA CACCACGCTG CAGAACACAC TGCGGAGAGC
    TACATGCTGC AGTGGCAGCG AGGGCACCTT TCCAACTACC AGTACCTGCT CCACCTCAAC
    AACCTGGCCG ACCGCAGCTG CAATGACCTG TCCCAGTACC CTGTGTTTCC ATGGATAATA
    AGTGATTACT CCAGCCCAGA GCTAGATTTG TCAAATCCTG CAACCTTCCG AGATCTTAGT
    AAGCCAGTGG GAGCCCTAAA TGCGGAGCGA CTGGAGAGAC TGCTGACTCG ATACCAGGAA
    ATGCCTGAGC CAAGATTCAT GTATGGGAGT CACTACTCTT CTCCAGGCTA TGTGCTCTTT
    TACCTCGTTA GGATCGCACC AGAGTATATG CTGTGTCTAC AAAATGGAAG ATTTGATAAT
    GCAGATAGGA TGTTTAACAG TATTGCAGAA ACGTGGAAAA ACTGTTTGGA TGGTGCAACG
    GATTTTAAAG AGTTGATTCC AGAATTCTAT GATGAAGACG TCAGCTTTTT GGTCAATAGT
    CTGAAGCTGG ATCTGGGAAA GAGACAAGGC GGGCAGATGG TTGATGACGT GGACCTTCCA
    GCCTGGGCCT CAAGTCCCCA AGACTTCCTG CAGAAGAACA AGGATGCCTT GGAAAGCGGT
    TACGTGTCTG AGCACCTTCA TGAGTGGATT GATCTGATAT TTGGCTATAA GCAAAAAGGG
    AGTGAGGCCA TAGGGGCCCA TAACGTGTTT CATCCCCTGA CCTATGAAGG TGGTGTAGAC
    TTGAACAGCA TTGAGGATCC TGATGAAAAA GTAGCCATGC TGACCCAAAT TTTGGAATTT
    GGGCAGACAC CAAAACAGCT GTTTGTGACA CCACATCCTC GAAGGATCAC CCCCAAGTTT
    AAAAGTTTGT CCCAAGCCTC CAGCTACAAC GCTTCTCTGA CAGACTCCCC AGTTTCTCCA
    GGGGAGGAGT CGTTTGAAGA CCTGACTGAG GAAAGCAGAA CTCTGGCCTG GAGTAATATC
    GCCAAGCTGC AGCTACATGA GCAGTACAAG ATCCACAAAG AGGCGGTTAC AGGGATAGCA
    GTGTCTTGCA ATGGCTCTTC AGTCTTTACC ACCTCCCAAG ATTCCACTTT GAAGATGTTT
    TCTAAGGAAT CCAAAATGCT ACAAAGAAGT ATATCATTTT CCAATATGGC TTTATCCTCT
    TGTTTACTGT TGCCGGGAGA CACCACTGTC ATAAGTTCTT CTTGGGATAA CAACGTCTAT
    TTTTATTCCA TAGCATTTGG AAGACGCCAA GATACACTGA TGGGACATGA CGATGCTGTC
    AGTAAGATCT GTTGGCATAA CGACAGGCTG TACTCTGGAT CTTGGGACTC TACTGTGAAG
    GTGTGGTCCG GTGTTCCTGC AGAAATGCCA GGAACAAAAA GACACCAGTT TGACTTACTG
    GCTGAGCTTG AACATGATGT CAGCGTCAAT ACTATCAATT TAAATGCTGT GAGCACACTG
    TTAGTTTCTG GCACCAAAGA AGGCATGGTG AATATTTGGG ACCTCACTAC AGCCACTTTA
    TTACACCAGA CTTCATGCCA TTCAGGGACA GTATGTGATG CTGCTTTTAG CCCAGATAGT
    CGCCACATCC TCAGCACGGG CGTAGATGGA TGTCTCAATG TGATTGATGT GCAGACGGGC
    ATGCTCATCT CCTCCATGGC CTCAGAGGAG CCGCAGAGGT GCTTTGTCTG GGATGGAAAT
    TCTGTCTTAT CTGGAAGTCG GTCTGGTGAG CTGCTTGTTT GGGACCTCCT TGGAGCAAAA
    GTCAGTGAGC GGATTCAGGG CCACACTGGT GCTGTGACAT GCATGTGGAT GAACGAACAG
    TGTAGCAGTA TTATCACCGG AGGGGAAGAC AGACAAATTA TGTTCTGGAA ATTGCAGTAT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu32752
    Clone ID Related Accession (Same CDS sequence) XM_006537662.4 , XM_006537662.3
    Accession Version XM_006537662.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2871bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product protein FAN isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006537662.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGCGTTCA CCCGGAAGAG GCAGCGCGAG CAGCAGCTGC AGCTCTACTC CAAGGAGAGA 
    TGCGGGTTTG TCTGTGGGAG CCGGGATCCG ACGGTCACGT TGTTGCTGAG TGGATCCAGC
    GAAGCAGGCT ATGCCAGGAA ATGCAGGTTT TCCTTACTGC TTCTCAACTT GGAGGAGTAC
    TACTTTGAGC AGCACACAGC GTTCCATGTC CAGCACCAGG GCAGCCAGGA AGAAAGGAAA
    ATCAGAGGCT CCTTAAAGAT ATGTTCAAAA TCAGTGATTT TTGAACCAGA TGCAATATCT
    CAGCCCATTC TTAAGATTCC TTTGAGAGAC TGTTTAAAAA TAGGAAAACA TGGAGAAAAT
    GGAGCCAATA AACACTTTGC AAAGGCAAAA TCATGGGGTA TTTCACTCAT TTTCAGTCAG
    ATATACTTTA TTAAGGAGCA CAACATTGTG GCACCGTATA AGATAGAGCG GGGAAAAATG
    GAGTATGTCT TTGAACTGGA AGTTTCTGGG AAGGTGGAAG ATGTTGTGGA GACATTGCTA
    CAGCTGCACA GAGCATCCTG CCTTGATAAA CTTGGTGACC AAATGGCCAT GATAACAGCT
    ATCTTGCAGT CACGTCTGGC TAGGACATCA TTTGACAAAA ACAGGTTCCA AAGTGTTTCT
    GAAAAGCTGC ACATGGAGTG CAAAGCAGAG ATGGTAACAC CTCTAGTGAC GAACCCTGGG
    CATGTGTGCA TTACAGACAC CAGCCTGTAT TTCCAGCCTC TCAATGGGTA CCCGAAACCT
    GTGGTCCAGA TAACACTGCA GGATGTCCGG CGCATTTACA AAAGGAGGCA CGGCCTCATG
    CCTTTGGGCT TGGAAGTGTT TTGCACAGAT GACGACCTGT GTTCCGACAT ATACCTAAAG
    TTCTATGAGC CTCAAGACAG AGATGATCTC TATTTTTATA TCGCCACGTA CCTAGAACAC
    CACGCTGCAG AACACACTGC GGAGAGCTAC ATGCTGCAGT GGCAGCGAGG GCACCTTTCC
    AACTACCAGT ACCTGCTCCA CCTCAACAAC CTGGCCGACC GCAGCTGCAA TGACCTGTCC
    CAGTACCCTG TGTTTCCATG GATAATAAGT GATTACTCCA GCCCAGAGCT AGATTTGTCA
    AATCCTGCAA CCTTCCGAGA TCTTAGTAAG CCAGTGGGAG CCCTAAATGC GGAGCGACTG
    GAGAGACTGC TGACTCGATA CCAGGAAATG CCTGAGCCAA GATTCATGTA TGGGAGTCAC
    TACTCTTCTC CAGGCTATGT GCTCTTTTAC CTCGTTAGGA TCGCACCAGA GTATATGCTG
    TGTCTACAAA ATGGAAGATT TGATAATGCA GATAGGATGT TTAACAGTAT TGCAGAAACG
    TGGAAAAACT GTTTGGATGG TGCAACGGAT TTTAAAGAGT TGATTCCAGA ATTCTATGAT
    GAAGACGTCA GCTTTTTGGT CAATAGTCTG AAGCTGGATC TGGGAAAGAG ACAAGGCGGG
    CAGATGGTTG ATGACGTGGA CCTTCCAGCC TGGGCCTCAA GTCCCCAAGA CTTCCTGCAG
    AAGAACAAGG ATGCCTTGGA AAGCGGTTAC GTGTCTGAGC ACCTTCATGA GTGGATTGAT
    CTGATATTTG GCTATAAGCA AAAAGGGAGT GAGGCCATAG GGGCCCATAA CGTGTTTCAT
    CCCCTGACCT ATGAAGGTGG TGTAGACTTG AACAGCATTG AGGATCCTGA TGAAAAAGTA
    GCCATGCTGA CCCAAATTTT GGAATTTGGG CAGACACCAA AACAGCTGTT TGTGACACCA
    CATCCTCGAA GGATCACCCC CAAGTTTAAA AGTTTGTCCC AAGCCTCCAG CTACAACGCT
    TCTCTGACAG ACTCCCCAGT TTCTCCAGGG GAGGAGTCGT TTGAAGACCT GACTGAGGAA
    AGCAGAACTC TGGCCTGGAG TAATATCGCC AAGCTGCAGC TACATGAGCA GTACAAGATC
    CACAAAGAGG CGGTTACAGG GATAGCAGTG TCTTGCAATG GCTCTTCAGT CTTTACCACC
    TCCCAAGATT CCACTTTGAA GATGTTTTCT AAGGAATCCA AAATGCTACA AAGAAGTATA
    TCATTTTCCA ATATGGCTTT ATCCTCTTGT TTACTGTTGC CGGGAGACAC CACTGTCATA
    AGTTCTTCTT GGGATAACAA CGTCTATTTT TATTCCATAG CATTTGGAAG ACGCCAAGAT
    ACACTGATGG GACATGACGA TGCTGTCAGT AAGATCTGTT GGCATAACGA CAGGCTGTAC
    TCTGGATCTT GGGACTCTAC TGTGAAGGTG TGGTCCGGTG TTCCTGCAGA AATGCCAGGA
    ACAAAAAGAC ACCAGTTTGA CTTACTGGCT GAGCTTGAAC ATGATGTCAG CATGACTTAT
    CTACTGGAGA AGGTCAATAC TATCAATTTA AATGCTGTGA GCACACTGTT AGTTTCTGGC
    ACCAAAGAAG GCATGGTGAA TATTTGGGAC CTCACTACAG CCACTTTATT ACACCAGACT
    TCATGCCATT CAGGGACAGT ATGTGATGCT GCTTTTAGCC CAGATAGTCG CCACATCCTC
    AGCACGGGCG TAGATGGATG TCTCAATGTG ATTGATGTGC AGACGGGCAT GCTCATCTCC
    TCCATGGCCT CAGAGGAGCC GCAGAGGTGC TTTGTCTGGG ATGGAAATTC TGTCTTATCT
    GGAAGTCGGT CTGGTGAGCT GCTTGTTTGG GACCTCCTTG GAGCAAAAGT CAGTGAGCGG
    ATTCAGGGCC ACACTGGTGC TGTGACATGC ATGTGGATGA ACGAACAGTG TAGCAGTATT
    ATCACCGGAG GGGAAGACAG ACAAATTATG TTCTGGAAAT TGCAGTATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006537725.1
    CDS626..3496
    Translation

    Target ORF information:

    RefSeq Version XM_006537662.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus neutral sphingomyelinase (N-SMase) activation associated factor (Nsmaf), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006537662.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGCGTTCA CCCGGAAGAG GCAGCGCGAG CAGCAGCTGC AGCTCTACTC CAAGGAGAGA 
    TGCGGGTTTG TCTGTGGGAG CCGGGATCCG ACGGTCACGT TGTTGCTGAG TGGATCCAGC
    GAAGCAGGCT ATGCCAGGAA ATGCAGGTTT TCCTTACTGC TTCTCAACTT GGAGGAGTAC
    TACTTTGAGC AGCACACAGC GTTCCATGTC CAGCACCAGG GCAGCCAGGA AGAAAGGAAA
    ATCAGAGGCT CCTTAAAGAT ATGTTCAAAA TCAGTGATTT TTGAACCAGA TGCAATATCT
    CAGCCCATTC TTAAGATTCC TTTGAGAGAC TGTTTAAAAA TAGGAAAACA TGGAGAAAAT
    GGAGCCAATA AACACTTTGC AAAGGCAAAA TCATGGGGTA TTTCACTCAT TTTCAGTCAG
    ATATACTTTA TTAAGGAGCA CAACATTGTG GCACCGTATA AGATAGAGCG GGGAAAAATG
    GAGTATGTCT TTGAACTGGA AGTTTCTGGG AAGGTGGAAG ATGTTGTGGA GACATTGCTA
    CAGCTGCACA GAGCATCCTG CCTTGATAAA CTTGGTGACC AAATGGCCAT GATAACAGCT
    ATCTTGCAGT CACGTCTGGC TAGGACATCA TTTGACAAAA ACAGGTTCCA AAGTGTTTCT
    GAAAAGCTGC ACATGGAGTG CAAAGCAGAG ATGGTAACAC CTCTAGTGAC GAACCCTGGG
    CATGTGTGCA TTACAGACAC CAGCCTGTAT TTCCAGCCTC TCAATGGGTA CCCGAAACCT
    GTGGTCCAGA TAACACTGCA GGATGTCCGG CGCATTTACA AAAGGAGGCA CGGCCTCATG
    CCTTTGGGCT TGGAAGTGTT TTGCACAGAT GACGACCTGT GTTCCGACAT ATACCTAAAG
    TTCTATGAGC CTCAAGACAG AGATGATCTC TATTTTTATA TCGCCACGTA CCTAGAACAC
    CACGCTGCAG AACACACTGC GGAGAGCTAC ATGCTGCAGT GGCAGCGAGG GCACCTTTCC
    AACTACCAGT ACCTGCTCCA CCTCAACAAC CTGGCCGACC GCAGCTGCAA TGACCTGTCC
    CAGTACCCTG TGTTTCCATG GATAATAAGT GATTACTCCA GCCCAGAGCT AGATTTGTCA
    AATCCTGCAA CCTTCCGAGA TCTTAGTAAG CCAGTGGGAG CCCTAAATGC GGAGCGACTG
    GAGAGACTGC TGACTCGATA CCAGGAAATG CCTGAGCCAA GATTCATGTA TGGGAGTCAC
    TACTCTTCTC CAGGCTATGT GCTCTTTTAC CTCGTTAGGA TCGCACCAGA GTATATGCTG
    TGTCTACAAA ATGGAAGATT TGATAATGCA GATAGGATGT TTAACAGTAT TGCAGAAACG
    TGGAAAAACT GTTTGGATGG TGCAACGGAT TTTAAAGAGT TGATTCCAGA ATTCTATGAT
    GAAGACGTCA GCTTTTTGGT CAATAGTCTG AAGCTGGATC TGGGAAAGAG ACAAGGCGGG
    CAGATGGTTG ATGACGTGGA CCTTCCAGCC TGGGCCTCAA GTCCCCAAGA CTTCCTGCAG
    AAGAACAAGG ATGCCTTGGA AAGCGGTTAC GTGTCTGAGC ACCTTCATGA GTGGATTGAT
    CTGATATTTG GCTATAAGCA AAAAGGGAGT GAGGCCATAG GGGCCCATAA CGTGTTTCAT
    CCCCTGACCT ATGAAGGTGG TGTAGACTTG AACAGCATTG AGGATCCTGA TGAAAAAGTA
    GCCATGCTGA CCCAAATTTT GGAATTTGGG CAGACACCAA AACAGCTGTT TGTGACACCA
    CATCCTCGAA GGATCACCCC CAAGTTTAAA AGTTTGTCCC AAGCCTCCAG CTACAACGCT
    TCTCTGACAG ACTCCCCAGT TTCTCCAGGG GAGGAGTCGT TTGAAGACCT GACTGAGGAA
    AGCAGAACTC TGGCCTGGAG TAATATCGCC AAGCTGCAGC TACATGAGCA GTACAAGATC
    CACAAAGAGG CGGTTACAGG GATAGCAGTG TCTTGCAATG GCTCTTCAGT CTTTACCACC
    TCCCAAGATT CCACTTTGAA GATGTTTTCT AAGGAATCCA AAATGCTACA AAGAAGTATA
    TCATTTTCCA ATATGGCTTT ATCCTCTTGT TTACTGTTGC CGGGAGACAC CACTGTCATA
    AGTTCTTCTT GGGATAACAA CGTCTATTTT TATTCCATAG CATTTGGAAG ACGCCAAGAT
    ACACTGATGG GACATGACGA TGCTGTCAGT AAGATCTGTT GGCATAACGA CAGGCTGTAC
    TCTGGATCTT GGGACTCTAC TGTGAAGGTG TGGTCCGGTG TTCCTGCAGA AATGCCAGGA
    ACAAAAAGAC ACCAGTTTGA CTTACTGGCT GAGCTTGAAC ATGATGTCAG CATGACTTAT
    CTACTGGAGA AGGTCAATAC TATCAATTTA AATGCTGTGA GCACACTGTT AGTTTCTGGC
    ACCAAAGAAG GCATGGTGAA TATTTGGGAC CTCACTACAG CCACTTTATT ACACCAGACT
    TCATGCCATT CAGGGACAGT ATGTGATGCT GCTTTTAGCC CAGATAGTCG CCACATCCTC
    AGCACGGGCG TAGATGGATG TCTCAATGTG ATTGATGTGC AGACGGGCAT GCTCATCTCC
    TCCATGGCCT CAGAGGAGCC GCAGAGGTGC TTTGTCTGGG ATGGAAATTC TGTCTTATCT
    GGAAGTCGGT CTGGTGAGCT GCTTGTTTGG GACCTCCTTG GAGCAAAAGT CAGTGAGCGG
    ATTCAGGGCC ACACTGGTGC TGTGACATGC ATGTGGATGA ACGAACAGTG TAGCAGTATT
    ATCACCGGAG GGGAAGACAG ACAAATTATG TTCTGGAAAT TGCAGTATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu32753
    Clone ID Related Accession (Same CDS sequence) XM_006537663.2 , XM_006537663.3
    Accession Version XM_006537663.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2850bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product protein FAN isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006537663.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGCGTTCA CCCGGAAGAG GCAGCGCGAG CAGCAGCTGC AGCTCTACTC CAAGGAGAGA 
    TGCGGGTTTG TCTGTGGGAG CCGGGATCCG ACGGTCACGT TGTTGCTGAG TGGATCCAGC
    GAAGCAGGCT ATGCCAGGAA ATGCAGGTTT TCCTTACTGC TTCTCAACTT GGAGGAGTAC
    TACTTTGAGC AGCACACAGC GTTCCATGTC CAGCACCAGG GCAGCCAGGA AGAAAGGAAA
    ATCAGAGGCT CCTTAAAGAT ATGTTCAAAA TCAGTGATTT TTGAACCAGA TGCAATATCT
    CAGCCCATTC TTAAGATTCC TTTGAGAGAC TGTTTAAAAA TAGGAAAACA TGGAGAAAAT
    GGAGCCAATA AACACTTTGC AAAGGCAAAA TCATGGGGTA TTTCACTCAT TTTCAGTCAG
    ATATACTTTA TTAAGGAGCA CAACATTGTG GCACCGTATA AGATAGAGCG GGGAAAAATG
    GAGTATGTCT TTGAACTGGA AGTTTCTGGG AAGGTGGAAG ATGTTGTGGA GACATTGCTA
    CAGCTGCACA GAGCATCCTG CCTTGATAAA CTTGGTGACC AAATGGCCAT GATAACAGCT
    ATCTTGCAGT CACGTCTGGC TAGGACATCA TTTGACAAAA ACAGGTTCCA AAGTGTTTCT
    GAAAAGCTGC ACATGGAGTG CAAAGCAGAG ATGGTAACAC CTCTAGTGAC GAACCCTGGG
    CATGTGTGCA TTACAGACAC CAGCCTGTAT TTCCAGCCTC TCAATGGGTA CCCGAAACCT
    GTGGTCCAGA TAACACTGCA GGATGTCCGG CGCATTTACA AAAGGAGGCA CGGCCTCATG
    CCTTTGGGCT TGGAAGTGTT TTGCACAGAT GACGACCTGT GTTCCGACAT ATACCTAAAG
    TTCTATGAGC CTCAAGACAG AGATGATCTC TATTTTTATA TCGCCACGTA CCTAGAACAC
    CACGCTGCAG AACACACTGC GGAGAGCTAC ATGCTGCAGT GGCAGCGAGG GCACCTTTCC
    AACTACCAGT ACCTGCTCCA CCTCAACAAC CTGGCCGACC GCAGCTGCAA TGACCTGTCC
    CAGTACCCTG TGTTTCCATG GATAATAAGT GATTACTCCA GCCCAGAGCT AGATTTGTCA
    AATCCTGCAA CCTTCCGAGA TCTTAGTAAG CCAGTGGGAG CCCTAAATGC GGAGCGACTG
    GAGAGACTGC TGACTCGATA CCAGGAAATG CCTGAGCCAA GATTCATGTA TGGGAGTCAC
    TACTCTTCTC CAGGCTATGT GCTCTTTTAC CTCGTTAGGA TCGCACCAGA GTATATGCTG
    TGTCTACAAA ATGGAAGATT TGATAATGCA GATAGGATGT TTAACAGTAT TGCAGAAACG
    TGGAAAAACT GTTTGGATGG TGCAACGGAT TTTAAAGAGT TGATTCCAGA ATTCTATGAT
    GAAGACGTCA GCTTTTTGGT CAATAGTCTG AAGCTGGATC TGGGAAAGAG ACAAGGCGGG
    CAGATGGTTG ATGACGTGGA CCTTCCAGCC TGGGCCTCAA GTCCCCAAGA CTTCCTGCAG
    AAGAACAAGG ATGCCTTGGA AAGCGGTTAC GTGTCTGAGC ACCTTCATGA GTGGATTGAT
    CTGATATTTG GCTATAAGCA AAAAGGGAGT GAGGCCATAG GGGCCCATAA CGTGTTTCAT
    CCCCTGACCT ATGAAGGTGG TGTAGACTTG AACAGCATTG AGGATCCTGA TGAAAAAGTA
    GCCATGCTGA CCCAAATTTT GGAATTTGGG CAGACACCAA AACAGCTGTT TGTGACACCA
    CATCCTCGAA GGATCACCCC CAAGTTTAAA AGTTTGTCCC AAGCCTCCAG CTACAACGCT
    TCTCTGACAG ACTCCCCAGT TTCTCCAGGG GAGGAGTCGT TTGAAGACCT GACTGAGGAA
    AGCAGAACTC TGGCCTGGAG TAATATCGCC AAGCTGCAGC TACATGAGCA GTACAAGATC
    CACAAAGAGG CGGTTACAGG GATAGCAGTG TCTTGCAATG GCTCTTCAGT CTTTACCACC
    TCCCAAGATT CCACTTTGAA GATGTTTTCT AAGGAATCCA AAATGCTACA AAGAAGTATA
    TCATTTTCCA ATATGGCTTT ATCCTCTTGT TTACTGTTGC CGGGAGACAC CACTGTCATA
    AGTTCTTCTT GGGATAACAA CGTCTATTTT TATTCCATAG CATTTGGAAG ACGCCAAGAT
    ACACTGATGG GACATGACGA TGCTGTCAGT AAGATCTGTT GGCATAACGA CAGGCTGTAC
    TCTGGATCTT GGGACTCTAC TGTGAAGGTG TGGTCCGGTG TTCCTGCAGA AATGCCAGGA
    ACAAAAAGAC ACCAGTTTGA CTTACTGGCT GAGCTTGAAC ATGATGTCAG CGTCAATACT
    ATCAATTTAA ATGCTGTGAG CACACTGTTA GTTTCTGGCA CCAAAGAAGG CATGGTGAAT
    ATTTGGGACC TCACTACAGC CACTTTATTA CACCAGACTT CATGCCATTC AGGGACAGTA
    TGTGATGCTG CTTTTAGCCC AGATAGTCGC CACATCCTCA GCACGGGCGT AGATGGATGT
    CTCAATGTGA TTGATGTGCA GACGGGCATG CTCATCTCCT CCATGGCCTC AGAGGAGCCG
    CAGAGGTGCT TTGTCTGGGA TGGAAATTCT GTCTTATCTG GAAGTCGGTC TGGTGAGCTG
    CTTGTTTGGG ACCTCCTTGG AGCAAAAGTC AGTGAGCGGA TTCAGGGCCA CACTGGTGCT
    GTGACATGCA TGTGGATGAA CGAACAGTGT AGCAGTATTA TCACCGGAGG GGAAGACAGA
    CAAATTATGT TCTGGAAATT GCAGTATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006537726.1
    CDS625..3474
    Translation

    Target ORF information:

    RefSeq Version XM_006537663.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus neutral sphingomyelinase (N-SMase) activation associated factor (Nsmaf), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006537663.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGCGTTCA CCCGGAAGAG GCAGCGCGAG CAGCAGCTGC AGCTCTACTC CAAGGAGAGA 
    TGCGGGTTTG TCTGTGGGAG CCGGGATCCG ACGGTCACGT TGTTGCTGAG TGGATCCAGC
    GAAGCAGGCT ATGCCAGGAA ATGCAGGTTT TCCTTACTGC TTCTCAACTT GGAGGAGTAC
    TACTTTGAGC AGCACACAGC GTTCCATGTC CAGCACCAGG GCAGCCAGGA AGAAAGGAAA
    ATCAGAGGCT CCTTAAAGAT ATGTTCAAAA TCAGTGATTT TTGAACCAGA TGCAATATCT
    CAGCCCATTC TTAAGATTCC TTTGAGAGAC TGTTTAAAAA TAGGAAAACA TGGAGAAAAT
    GGAGCCAATA AACACTTTGC AAAGGCAAAA TCATGGGGTA TTTCACTCAT TTTCAGTCAG
    ATATACTTTA TTAAGGAGCA CAACATTGTG GCACCGTATA AGATAGAGCG GGGAAAAATG
    GAGTATGTCT TTGAACTGGA AGTTTCTGGG AAGGTGGAAG ATGTTGTGGA GACATTGCTA
    CAGCTGCACA GAGCATCCTG CCTTGATAAA CTTGGTGACC AAATGGCCAT GATAACAGCT
    ATCTTGCAGT CACGTCTGGC TAGGACATCA TTTGACAAAA ACAGGTTCCA AAGTGTTTCT
    GAAAAGCTGC ACATGGAGTG CAAAGCAGAG ATGGTAACAC CTCTAGTGAC GAACCCTGGG
    CATGTGTGCA TTACAGACAC CAGCCTGTAT TTCCAGCCTC TCAATGGGTA CCCGAAACCT
    GTGGTCCAGA TAACACTGCA GGATGTCCGG CGCATTTACA AAAGGAGGCA CGGCCTCATG
    CCTTTGGGCT TGGAAGTGTT TTGCACAGAT GACGACCTGT GTTCCGACAT ATACCTAAAG
    TTCTATGAGC CTCAAGACAG AGATGATCTC TATTTTTATA TCGCCACGTA CCTAGAACAC
    CACGCTGCAG AACACACTGC GGAGAGCTAC ATGCTGCAGT GGCAGCGAGG GCACCTTTCC
    AACTACCAGT ACCTGCTCCA CCTCAACAAC CTGGCCGACC GCAGCTGCAA TGACCTGTCC
    CAGTACCCTG TGTTTCCATG GATAATAAGT GATTACTCCA GCCCAGAGCT AGATTTGTCA
    AATCCTGCAA CCTTCCGAGA TCTTAGTAAG CCAGTGGGAG CCCTAAATGC GGAGCGACTG
    GAGAGACTGC TGACTCGATA CCAGGAAATG CCTGAGCCAA GATTCATGTA TGGGAGTCAC
    TACTCTTCTC CAGGCTATGT GCTCTTTTAC CTCGTTAGGA TCGCACCAGA GTATATGCTG
    TGTCTACAAA ATGGAAGATT TGATAATGCA GATAGGATGT TTAACAGTAT TGCAGAAACG
    TGGAAAAACT GTTTGGATGG TGCAACGGAT TTTAAAGAGT TGATTCCAGA ATTCTATGAT
    GAAGACGTCA GCTTTTTGGT CAATAGTCTG AAGCTGGATC TGGGAAAGAG ACAAGGCGGG
    CAGATGGTTG ATGACGTGGA CCTTCCAGCC TGGGCCTCAA GTCCCCAAGA CTTCCTGCAG
    AAGAACAAGG ATGCCTTGGA AAGCGGTTAC GTGTCTGAGC ACCTTCATGA GTGGATTGAT
    CTGATATTTG GCTATAAGCA AAAAGGGAGT GAGGCCATAG GGGCCCATAA CGTGTTTCAT
    CCCCTGACCT ATGAAGGTGG TGTAGACTTG AACAGCATTG AGGATCCTGA TGAAAAAGTA
    GCCATGCTGA CCCAAATTTT GGAATTTGGG CAGACACCAA AACAGCTGTT TGTGACACCA
    CATCCTCGAA GGATCACCCC CAAGTTTAAA AGTTTGTCCC AAGCCTCCAG CTACAACGCT
    TCTCTGACAG ACTCCCCAGT TTCTCCAGGG GAGGAGTCGT TTGAAGACCT GACTGAGGAA
    AGCAGAACTC TGGCCTGGAG TAATATCGCC AAGCTGCAGC TACATGAGCA GTACAAGATC
    CACAAAGAGG CGGTTACAGG GATAGCAGTG TCTTGCAATG GCTCTTCAGT CTTTACCACC
    TCCCAAGATT CCACTTTGAA GATGTTTTCT AAGGAATCCA AAATGCTACA AAGAAGTATA
    TCATTTTCCA ATATGGCTTT ATCCTCTTGT TTACTGTTGC CGGGAGACAC CACTGTCATA
    AGTTCTTCTT GGGATAACAA CGTCTATTTT TATTCCATAG CATTTGGAAG ACGCCAAGAT
    ACACTGATGG GACATGACGA TGCTGTCAGT AAGATCTGTT GGCATAACGA CAGGCTGTAC
    TCTGGATCTT GGGACTCTAC TGTGAAGGTG TGGTCCGGTG TTCCTGCAGA AATGCCAGGA
    ACAAAAAGAC ACCAGTTTGA CTTACTGGCT GAGCTTGAAC ATGATGTCAG CGTCAATACT
    ATCAATTTAA ATGCTGTGAG CACACTGTTA GTTTCTGGCA CCAAAGAAGG CATGGTGAAT
    ATTTGGGACC TCACTACAGC CACTTTATTA CACCAGACTT CATGCCATTC AGGGACAGTA
    TGTGATGCTG CTTTTAGCCC AGATAGTCGC CACATCCTCA GCACGGGCGT AGATGGATGT
    CTCAATGTGA TTGATGTGCA GACGGGCATG CTCATCTCCT CCATGGCCTC AGAGGAGCCG
    CAGAGGTGCT TTGTCTGGGA TGGAAATTCT GTCTTATCTG GAAGTCGGTC TGGTGAGCTG
    CTTGTTTGGG ACCTCCTTGG AGCAAAAGTC AGTGAGCGGA TTCAGGGCCA CACTGGTGCT
    GTGACATGCA TGTGGATGAA CGAACAGTGT AGCAGTATTA TCACCGGAGG GGAAGACAGA
    CAAATTATGT TCTGGAAATT GCAGTATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu32754
    Clone ID Related Accession (Same CDS sequence) XM_006537664.2 , XM_006537664.3
    Accession Version XM_006537664.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2784bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product protein FAN isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Feb 9, 2015 this sequence version replaced XM_006537664.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCGTTCA CCCGGAAGAG GCAGCGCGAG CAGCAGCTGC AGCTCTACTC CAAGGAGAGG 
    TTTTCCTTAC TGCTTCTCAA CTTGGAGGAG TACTACTTTG AGCAGCACAC AGCGTTCCAT
    GTCCAGCACC AGGGCAGCCA GGAAGAAAGG AAAATCAGAG GCTCCTTAAA GATATGTTCA
    AAATCAGTGA TTTTTGAACC AGATGCAATA TCTCAGCCCA TTCTTAAGAT TCCTTTGAGA
    GACTGTTTAA AAATAGGAAA ACATGGAGAA AATGGAGCCA ATAAACACTT TGCAAAGGCA
    AAATCATGGG GTATTTCACT CATTTTCAGT CAGATATACT TTATTAAGGA GCACAACATT
    GTGGCACCGT ATAAGATAGA GCGGGGAAAA ATGGAGTATG TCTTTGAACT GGAAGTTTCT
    GGGAAGGTGG AAGATGTTGT GGAGACATTG CTACAGCTGC ACAGAGCATC CTGCCTTGAT
    AAACTTGGTG ACCAAATGGC CATGATAACA GCTATCTTGC AGTCACGTCT GGCTAGGACA
    TCATTTGACA AAAACAGGTT CCAAAGTGTT TCTGAAAAGC TGCACATGGA GTGCAAAGCA
    GAGATGGTAA CACCTCTAGT GACGAACCCT GGGCATGTGT GCATTACAGA CACCAGCCTG
    TATTTCCAGC CTCTCAATGG GTACCCGAAA CCTGTGGTCC AGATAACACT GCAGGATGTC
    CGGCGCATTT ACAAAAGGAG GCACGGCCTC ATGCCTTTGG GCTTGGAAGT GTTTTGCACA
    GATGACGACC TGTGTTCCGA CATATACCTA AAGTTCTATG AGCCTCAAGA CAGAGATGAT
    CTCTATTTTT ATATCGCCAC GTACCTAGAA CACCACGCTG CAGAACACAC TGCGGAGAGC
    TACATGCTGC AGTGGCAGCG AGGGCACCTT TCCAACTACC AGTACCTGCT CCACCTCAAC
    AACCTGGCCG ACCGCAGCTG CAATGACCTG TCCCAGTACC CTGTGTTTCC ATGGATAATA
    AGTGATTACT CCAGCCCAGA GCTAGATTTG TCAAATCCTG CAACCTTCCG AGATCTTAGT
    AAGCCAGTGG GAGCCCTAAA TGCGGAGCGA CTGGAGAGAC TGCTGACTCG ATACCAGGAA
    ATGCCTGAGC CAAGATTCAT GTATGGGAGT CACTACTCTT CTCCAGGCTA TGTGCTCTTT
    TACCTCGTTA GGATCGCACC AGAGTATATG CTGTGTCTAC AAAATGGAAG ATTTGATAAT
    GCAGATAGGA TGTTTAACAG TATTGCAGAA ACGTGGAAAA ACTGTTTGGA TGGTGCAACG
    GATTTTAAAG AGTTGATTCC AGAATTCTAT GATGAAGACG TCAGCTTTTT GGTCAATAGT
    CTGAAGCTGG ATCTGGGAAA GAGACAAGGC GGGCAGATGG TTGATGACGT GGACCTTCCA
    GCCTGGGCCT CAAGTCCCCA AGACTTCCTG CAGAAGAACA AGGATGCCTT GGAAAGCGGT
    TACGTGTCTG AGCACCTTCA TGAGTGGATT GATCTGATAT TTGGCTATAA GCAAAAAGGG
    AGTGAGGCCA TAGGGGCCCA TAACGTGTTT CATCCCCTGA CCTATGAAGG TGGTGTAGAC
    TTGAACAGCA TTGAGGATCC TGATGAAAAA GTAGCCATGC TGACCCAAAT TTTGGAATTT
    GGGCAGACAC CAAAACAGCT GTTTGTGACA CCACATCCTC GAAGGATCAC CCCCAAGTTT
    AAAAGTTTGT CCCAAGCCTC CAGCTACAAC GCTTCTCTGA CAGACTCCCC AGTTTCTCCA
    GGGGAGGAGT CGTTTGAAGA CCTGACTGAG GAAAGCAGAA CTCTGGCCTG GAGTAATATC
    GCCAAGCTGC AGCTACATGA GCAGTACAAG ATCCACAAAG AGGCGGTTAC AGGGATAGCA
    GTGTCTTGCA ATGGCTCTTC AGTCTTTACC ACCTCCCAAG ATTCCACTTT GAAGATGTTT
    TCTAAGGAAT CCAAAATGCT ACAAAGAAGT ATATCATTTT CCAATATGGC TTTATCCTCT
    TGTTTACTGT TGCCGGGAGA CACCACTGTC ATAAGTTCTT CTTGGGATAA CAACGTCTAT
    TTTTATTCCA TAGCATTTGG AAGACGCCAA GATACACTGA TGGGACATGA CGATGCTGTC
    AGTAAGATCT GTTGGCATAA CGACAGGCTG TACTCTGGAT CTTGGGACTC TACTGTGAAG
    GTGTGGTCCG GTGTTCCTGC AGAAATGCCA GGAACAAAAA GACACCAGTT TGACTTACTG
    GCTGAGCTTG AACATGATGT CAGCATGACT TATCTACTGG AGAAGGTCAA TACTATCAAT
    TTAAATGCTG TGAGCACACT GTTAGTTTCT GGCACCAAAG AAGGCATGGT GAATATTTGG
    GACCTCACTA CAGCCACTTT ATTACACCAG ACTTCATGCC ATTCAGGGAC AGTATGTGAT
    GCTGCTTTTA GCCCAGATAG TCGCCACATC CTCAGCACGG GCGTAGATGG ATGTCTCAAT
    GTGATTGATG TGCAGACGGG CATGCTCATC TCCTCCATGG CCTCAGAGGA GCCGCAGAGG
    TGCTTTGTCT GGGATGGAAA TTCTGTCTTA TCTGGAAGTC GGTCTGGTGA GCTGCTTGTT
    TGGGACCTCC TTGGAGCAAA AGTCAGTGAG CGGATTCAGG GCCACACTGG TGCTGTGACA
    TGCATGTGGA TGAACGAACA GTGTAGCAGT ATTATCACCG GAGGGGAAGA CAGACAAATT
    ATGTTCTGGA AATTGCAGTA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006537727.1
    CDS625..3408
    Translation

    Target ORF information:

    RefSeq Version XM_006537664.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus neutral sphingomyelinase (N-SMase) activation associated factor (Nsmaf), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006537664.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCGTTCA CCCGGAAGAG GCAGCGCGAG CAGCAGCTGC AGCTCTACTC CAAGGAGAGG 
    TTTTCCTTAC TGCTTCTCAA CTTGGAGGAG TACTACTTTG AGCAGCACAC AGCGTTCCAT
    GTCCAGCACC AGGGCAGCCA GGAAGAAAGG AAAATCAGAG GCTCCTTAAA GATATGTTCA
    AAATCAGTGA TTTTTGAACC AGATGCAATA TCTCAGCCCA TTCTTAAGAT TCCTTTGAGA
    GACTGTTTAA AAATAGGAAA ACATGGAGAA AATGGAGCCA ATAAACACTT TGCAAAGGCA
    AAATCATGGG GTATTTCACT CATTTTCAGT CAGATATACT TTATTAAGGA GCACAACATT
    GTGGCACCGT ATAAGATAGA GCGGGGAAAA ATGGAGTATG TCTTTGAACT GGAAGTTTCT
    GGGAAGGTGG AAGATGTTGT GGAGACATTG CTACAGCTGC ACAGAGCATC CTGCCTTGAT
    AAACTTGGTG ACCAAATGGC CATGATAACA GCTATCTTGC AGTCACGTCT GGCTAGGACA
    TCATTTGACA AAAACAGGTT CCAAAGTGTT TCTGAAAAGC TGCACATGGA GTGCAAAGCA
    GAGATGGTAA CACCTCTAGT GACGAACCCT GGGCATGTGT GCATTACAGA CACCAGCCTG
    TATTTCCAGC CTCTCAATGG GTACCCGAAA CCTGTGGTCC AGATAACACT GCAGGATGTC
    CGGCGCATTT ACAAAAGGAG GCACGGCCTC ATGCCTTTGG GCTTGGAAGT GTTTTGCACA
    GATGACGACC TGTGTTCCGA CATATACCTA AAGTTCTATG AGCCTCAAGA CAGAGATGAT
    CTCTATTTTT ATATCGCCAC GTACCTAGAA CACCACGCTG CAGAACACAC TGCGGAGAGC
    TACATGCTGC AGTGGCAGCG AGGGCACCTT TCCAACTACC AGTACCTGCT CCACCTCAAC
    AACCTGGCCG ACCGCAGCTG CAATGACCTG TCCCAGTACC CTGTGTTTCC ATGGATAATA
    AGTGATTACT CCAGCCCAGA GCTAGATTTG TCAAATCCTG CAACCTTCCG AGATCTTAGT
    AAGCCAGTGG GAGCCCTAAA TGCGGAGCGA CTGGAGAGAC TGCTGACTCG ATACCAGGAA
    ATGCCTGAGC CAAGATTCAT GTATGGGAGT CACTACTCTT CTCCAGGCTA TGTGCTCTTT
    TACCTCGTTA GGATCGCACC AGAGTATATG CTGTGTCTAC AAAATGGAAG ATTTGATAAT
    GCAGATAGGA TGTTTAACAG TATTGCAGAA ACGTGGAAAA ACTGTTTGGA TGGTGCAACG
    GATTTTAAAG AGTTGATTCC AGAATTCTAT GATGAAGACG TCAGCTTTTT GGTCAATAGT
    CTGAAGCTGG ATCTGGGAAA GAGACAAGGC GGGCAGATGG TTGATGACGT GGACCTTCCA
    GCCTGGGCCT CAAGTCCCCA AGACTTCCTG CAGAAGAACA AGGATGCCTT GGAAAGCGGT
    TACGTGTCTG AGCACCTTCA TGAGTGGATT GATCTGATAT TTGGCTATAA GCAAAAAGGG
    AGTGAGGCCA TAGGGGCCCA TAACGTGTTT CATCCCCTGA CCTATGAAGG TGGTGTAGAC
    TTGAACAGCA TTGAGGATCC TGATGAAAAA GTAGCCATGC TGACCCAAAT TTTGGAATTT
    GGGCAGACAC CAAAACAGCT GTTTGTGACA CCACATCCTC GAAGGATCAC CCCCAAGTTT
    AAAAGTTTGT CCCAAGCCTC CAGCTACAAC GCTTCTCTGA CAGACTCCCC AGTTTCTCCA
    GGGGAGGAGT CGTTTGAAGA CCTGACTGAG GAAAGCAGAA CTCTGGCCTG GAGTAATATC
    GCCAAGCTGC AGCTACATGA GCAGTACAAG ATCCACAAAG AGGCGGTTAC AGGGATAGCA
    GTGTCTTGCA ATGGCTCTTC AGTCTTTACC ACCTCCCAAG ATTCCACTTT GAAGATGTTT
    TCTAAGGAAT CCAAAATGCT ACAAAGAAGT ATATCATTTT CCAATATGGC TTTATCCTCT
    TGTTTACTGT TGCCGGGAGA CACCACTGTC ATAAGTTCTT CTTGGGATAA CAACGTCTAT
    TTTTATTCCA TAGCATTTGG AAGACGCCAA GATACACTGA TGGGACATGA CGATGCTGTC
    AGTAAGATCT GTTGGCATAA CGACAGGCTG TACTCTGGAT CTTGGGACTC TACTGTGAAG
    GTGTGGTCCG GTGTTCCTGC AGAAATGCCA GGAACAAAAA GACACCAGTT TGACTTACTG
    GCTGAGCTTG AACATGATGT CAGCATGACT TATCTACTGG AGAAGGTCAA TACTATCAAT
    TTAAATGCTG TGAGCACACT GTTAGTTTCT GGCACCAAAG AAGGCATGGT GAATATTTGG
    GACCTCACTA CAGCCACTTT ATTACACCAG ACTTCATGCC ATTCAGGGAC AGTATGTGAT
    GCTGCTTTTA GCCCAGATAG TCGCCACATC CTCAGCACGG GCGTAGATGG ATGTCTCAAT
    GTGATTGATG TGCAGACGGG CATGCTCATC TCCTCCATGG CCTCAGAGGA GCCGCAGAGG
    TGCTTTGTCT GGGATGGAAA TTCTGTCTTA TCTGGAAGTC GGTCTGGTGA GCTGCTTGTT
    TGGGACCTCC TTGGAGCAAA AGTCAGTGAG CGGATTCAGG GCCACACTGG TGCTGTGACA
    TGCATGTGGA TGAACGAACA GTGTAGCAGT ATTATCACCG GAGGGGAAGA CAGACAAATT
    ATGTTCTGGA AATTGCAGTA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu68335
    Clone ID Related Accession (Same CDS sequence) XM_011249943.2 , XM_011249943.3
    Accession Version XM_011249943.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2469bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product protein FAN isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011249943.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGCGTTCA CCCGGAAGAG GCAGCGCGAG CAGCAGCTGC AGCTCTACTC CAAGGAGAGA 
    TGCGGGTTTG TCTGTGGGAG CCGGGATCCG ACGGTCACGT TGTTGCTGAG TGGATCCAGC
    GAAGCAGGCT ATGCCAGGAA ATGCAGGTTT TCCTTACTGC TTCTCAACTT GGAGGAGTAC
    TACTTTGAGC AGCACACAGC GTTCCATGTC CAGCACCAGG GCAGCCAGGA AGAAAGGAAA
    ATCAGAGGCT CCTTAAAGAT ATGTTCAAAA TCAGTGATTT TTGAACCAGA TGCAATATCT
    CAGCCCATTC TTAAGATTCC TTTGAGAGAC TGTTTAAAAA TAGGAAAACA TGGAGAAAAT
    GGAGCCAATA AACACTTTGC AAAGGCAAAA TCATGGGGTA TTTCACTCAT TTTCAGTCAG
    ATATACTTTA TTAAGGAGCA CAACATTGTG GCACCGTATA AGATAGAGCG GGGAAAAATG
    GAGTATGTCT TTGAACTGGA AGTTTCTGGG AAGGTGGAAG ATGTTGTGGA GACATTGCTA
    CAGCTGCACA GAGCATCCTG CCTTGATAAA CTTGGTGACC AAATGGCCAT GATAACAGCT
    ATCTTGCAGT CACGTCTGGC TAGGACATCA TTTGACAAAA ACAGGTTCCA AAGTGTTTCT
    GAAAAGCTGC ACATGGAGTG CAAAGCAGAG ATGGTAACAC CTCTAGTGAC GAACCCTGGG
    CATGTGTGCA TTACAGACAC CAGCCTGTAT TTCCAGCCTC TCAATGGGTA CCCGAAACCT
    GTGGTCCAGA TAACACTGCA GGATGTCCGG CGCATTTACA AAAGGAGGCA CGGCCTCATG
    CCTTTGGGCT TGGAAGTGTT TTGCACAGAT GACGACCTGT GTTCCGACAT ATACCTAAAG
    TTCTATGAGC CTCAAGACAG AGATGATCTC TATTTTTATA TCGCCACGTA CCTAGAACAC
    CACGCTGCAG AACACACTGC GGAGAGCTAC ATGCTGCAGT GGCAGCGAGG GCACCTTTCC
    AACTACCAGT ACCTGCTCCA CCTCAACAAC CTGGCCGACC GCAGCTGCAA TGACCTGTCC
    CAGTACCCTG TGTTTCCATG GATAATAAGT GATTACTCCA GCCCAGAGCT AGATTTGTCA
    AATCCTGCAA CCTTCCGAGA TCTTAGTAAG CCAGTGGGAG CCCTAAATGC GGAGCGACTG
    GAGAGACTGC TGACTCGATA CCAGGAAATG CCTGAGCCAA GATTCATGTA TGGGAGTCAC
    TACTCTTCTC CAGGCTATGT GCTCTTTTAC CTCGTTAGGA TCGCACCAGA GTATATGCTG
    TGTCTACAAA ATGGAAGATT TGATAATGCA GATAGGATGT TTAACAGTAT TGCAGAAACG
    TGGAAAAACT GTTTGGATGG TGCAACGGAT TTTAAAGAGT TGATTCCAGA ATTCTATGAT
    GAAGACGTCA GCTTTTTGGT CAATAGTCTG AAGCTGGATC TGGGAAAGAG ACAAGGCGGG
    CAGATGGTTG ATGACGTGGA CCTTCCAGCC TGGGCCTCAA GTCCCCAAGA CTTCCTGCAG
    AAGAACAAGG ATGCCTTGGA AAGCGGTTAC GTGTCTGAGC ACCTTCATGA GTGGATTGAT
    CTGATATTTG GCTATAAGCA AAAAGGGAGT GAGGCCATAG GGGCCCATAA CGTGTTTCAT
    CCCCTGACCT ATGAAGGTGG TGTAGACTTG AACAGCATTG AGGATCCTGA TGAAAAAGTA
    GCCATGCTGA CCCAAATTTT GGAATTTGGG CAGACACCAA AACAGCTGTT TGTGACACCA
    CATCCTCGAA GGATCACCCC CAAGTTTAAA AGTTTGTCCC AAGCCTCCAG CTACAACGCT
    TCTCTGACAG ACTCCCCAGT TTCTCCAGGG GAGGAGTCGT TTGAAGACCT GACTGAGGAA
    AGCAGAACTC TGGCCTGGAG TAATATCGCC AAGCTGCAGC TACATGAGCA GTACAAGATC
    CACAAAGAGG CGGTTACAGG GATAGCAGTG TCTTGCAATG GCTCTTCAGT CTTTACCACC
    TCCCAAGATT CCACTTTGAA GATGTTTTCT AAGGAATCCA AAATGCTACA AAGAAGTATA
    TCATTTTCCA ATATGGCTTT ATCCTCTTGT TTACTGTTGC CGGGAGACAC CACTGTCATA
    AGTTCTTCTT GGGATAACAA CGTCTATTTT TATTCCATAG CATTTGGAAG ACGCCAAGAT
    ACACTGATGG GACATGACGA TGCTGTCAGT AAGATCTGTT GGCATAACGA CAGGCTGTAC
    TCTGGATCTT GGGACTCTAC TGTGAAGGTG TGGTCCGGTG TTCCTGCAGA AATGCCAGGA
    ACAAAAAGAC ACCAGTTTGA CTTACTGGCT GAGCTTGAAC ATGATGTCAG CATGACTTAT
    CTACTGGAGA AGAAAAGAAA TGCTGTACTT CGTGTCCCTC CTCTCCTCCA GTCAGAGTTT
    TGCTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011248245.1
    CDS631..3099
    Translation

    Target ORF information:

    RefSeq Version XM_011249943.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus neutral sphingomyelinase (N-SMase) activation associated factor (Nsmaf), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011249943.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGCGTTCA CCCGGAAGAG GCAGCGCGAG CAGCAGCTGC AGCTCTACTC CAAGGAGAGA 
    TGCGGGTTTG TCTGTGGGAG CCGGGATCCG ACGGTCACGT TGTTGCTGAG TGGATCCAGC
    GAAGCAGGCT ATGCCAGGAA ATGCAGGTTT TCCTTACTGC TTCTCAACTT GGAGGAGTAC
    TACTTTGAGC AGCACACAGC GTTCCATGTC CAGCACCAGG GCAGCCAGGA AGAAAGGAAA
    ATCAGAGGCT CCTTAAAGAT ATGTTCAAAA TCAGTGATTT TTGAACCAGA TGCAATATCT
    CAGCCCATTC TTAAGATTCC TTTGAGAGAC TGTTTAAAAA TAGGAAAACA TGGAGAAAAT
    GGAGCCAATA AACACTTTGC AAAGGCAAAA TCATGGGGTA TTTCACTCAT TTTCAGTCAG
    ATATACTTTA TTAAGGAGCA CAACATTGTG GCACCGTATA AGATAGAGCG GGGAAAAATG
    GAGTATGTCT TTGAACTGGA AGTTTCTGGG AAGGTGGAAG ATGTTGTGGA GACATTGCTA
    CAGCTGCACA GAGCATCCTG CCTTGATAAA CTTGGTGACC AAATGGCCAT GATAACAGCT
    ATCTTGCAGT CACGTCTGGC TAGGACATCA TTTGACAAAA ACAGGTTCCA AAGTGTTTCT
    GAAAAGCTGC ACATGGAGTG CAAAGCAGAG ATGGTAACAC CTCTAGTGAC GAACCCTGGG
    CATGTGTGCA TTACAGACAC CAGCCTGTAT TTCCAGCCTC TCAATGGGTA CCCGAAACCT
    GTGGTCCAGA TAACACTGCA GGATGTCCGG CGCATTTACA AAAGGAGGCA CGGCCTCATG
    CCTTTGGGCT TGGAAGTGTT TTGCACAGAT GACGACCTGT GTTCCGACAT ATACCTAAAG
    TTCTATGAGC CTCAAGACAG AGATGATCTC TATTTTTATA TCGCCACGTA CCTAGAACAC
    CACGCTGCAG AACACACTGC GGAGAGCTAC ATGCTGCAGT GGCAGCGAGG GCACCTTTCC
    AACTACCAGT ACCTGCTCCA CCTCAACAAC CTGGCCGACC GCAGCTGCAA TGACCTGTCC
    CAGTACCCTG TGTTTCCATG GATAATAAGT GATTACTCCA GCCCAGAGCT AGATTTGTCA
    AATCCTGCAA CCTTCCGAGA TCTTAGTAAG CCAGTGGGAG CCCTAAATGC GGAGCGACTG
    GAGAGACTGC TGACTCGATA CCAGGAAATG CCTGAGCCAA GATTCATGTA TGGGAGTCAC
    TACTCTTCTC CAGGCTATGT GCTCTTTTAC CTCGTTAGGA TCGCACCAGA GTATATGCTG
    TGTCTACAAA ATGGAAGATT TGATAATGCA GATAGGATGT TTAACAGTAT TGCAGAAACG
    TGGAAAAACT GTTTGGATGG TGCAACGGAT TTTAAAGAGT TGATTCCAGA ATTCTATGAT
    GAAGACGTCA GCTTTTTGGT CAATAGTCTG AAGCTGGATC TGGGAAAGAG ACAAGGCGGG
    CAGATGGTTG ATGACGTGGA CCTTCCAGCC TGGGCCTCAA GTCCCCAAGA CTTCCTGCAG
    AAGAACAAGG ATGCCTTGGA AAGCGGTTAC GTGTCTGAGC ACCTTCATGA GTGGATTGAT
    CTGATATTTG GCTATAAGCA AAAAGGGAGT GAGGCCATAG GGGCCCATAA CGTGTTTCAT
    CCCCTGACCT ATGAAGGTGG TGTAGACTTG AACAGCATTG AGGATCCTGA TGAAAAAGTA
    GCCATGCTGA CCCAAATTTT GGAATTTGGG CAGACACCAA AACAGCTGTT TGTGACACCA
    CATCCTCGAA GGATCACCCC CAAGTTTAAA AGTTTGTCCC AAGCCTCCAG CTACAACGCT
    TCTCTGACAG ACTCCCCAGT TTCTCCAGGG GAGGAGTCGT TTGAAGACCT GACTGAGGAA
    AGCAGAACTC TGGCCTGGAG TAATATCGCC AAGCTGCAGC TACATGAGCA GTACAAGATC
    CACAAAGAGG CGGTTACAGG GATAGCAGTG TCTTGCAATG GCTCTTCAGT CTTTACCACC
    TCCCAAGATT CCACTTTGAA GATGTTTTCT AAGGAATCCA AAATGCTACA AAGAAGTATA
    TCATTTTCCA ATATGGCTTT ATCCTCTTGT TTACTGTTGC CGGGAGACAC CACTGTCATA
    AGTTCTTCTT GGGATAACAA CGTCTATTTT TATTCCATAG CATTTGGAAG ACGCCAAGAT
    ACACTGATGG GACATGACGA TGCTGTCAGT AAGATCTGTT GGCATAACGA CAGGCTGTAC
    TCTGGATCTT GGGACTCTAC TGTGAAGGTG TGGTCCGGTG TTCCTGCAGA AATGCCAGGA
    ACAAAAAGAC ACCAGTTTGA CTTACTGGCT GAGCTTGAAC ATGATGTCAG CATGACTTAT
    CTACTGGAGA AGAAAAGAAA TGCTGTACTT CGTGTCCCTC CTCTCCTCCA GTCAGAGTTT
    TGCTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu68336
    Clone ID Related Accession (Same CDS sequence) XM_011249944.2 , XM_011249944.3
    Accession Version XM_011249944.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2415bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product protein FAN isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011249944.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGCGTTCA CCCGGAAGAG GCAGCGCGAG CAGCAGCTGC AGCTCTACTC CAAGGAGAGA 
    TGCGGGTTTG TCTGTGGGAG CCGGGATCCG ACGGTCACGT TGTTGCTGAG TGGATCCAGC
    GAAGCAGGCT ATGCCAGGAA ATGCAGGTTT TCCTTACTGC TTCTCAACTT GGAGGAGTAC
    TACTTTGAGC AGCACACAGC GTTCCATGTC CAGCACCAGG GCAGCCAGGA AGAAAGGAAA
    ATCAGAGGCT CCTTAAAGAT ATGTTCAAAA TCAGTGATTT TTGAACCAGA TGCAATATCT
    CAGCCCATTC TTAAGATTCC TTTGAGAGAC TGTTTAAAAA TAGGAAAACA TGGAGAAAAT
    GGAGCCAATA AACACTTTGC AAAGGCAAAA TCATGGGGTA TTTCACTCAT TTTCAGTCAG
    ATATACTTTA TTAAGGAGCA CAACATTGTG GCACCGTATA AGATAGAGCG GGGAAAAATG
    GAGTATGTCT TTGAACTGGA AGTTTCTGGG AAGGTGGAAG ATGTTGTGGA GACATTGCTA
    CAGCTGCACA GAGCATCCTG CCTTGATAAA CTTGGTGACC AAATGGCCAT GATAACAGCT
    ATCTTGCAGT CACGTCTGGC TAGGACATCA TTTGACAAAA ACAGGTTCCA AAGTGTTTCT
    GAAAAGCTGC ACATGGAGTG CAAAGCAGAG ATGGTAACAC CTCTAGTGAC GAACCCTGGG
    CATGTGTGCA TTACAGACAC CAGCCTGTAT TTCCAGCCTC TCAATGGGTA CCCGAAACCT
    GTGGTCCAGA TAACACTGCA GGATGTCCGG CGCATTTACA AAAGGAGGCA CGGCCTCATG
    CCTTTGGGCT TGGAAGTGTT TTGCACAGAT GACGACCTGT GTTCCGACAT ATACCTAAAG
    TTCTATGAGC CTCAAGACAG AGATGATCTC TATTTTTATA TCGCCACGTA CCTAGAACAC
    CACGCTGCAG AACACACTGC GGAGAGCTAC ATGCTGCAGT GGCAGCGAGG GCACCTTTCC
    AACTACCAGT ACCTGCTCCA CCTCAACAAC CTGGCCGACC GCAGCTGCAA TGACCTGTCC
    CAGTACCCTG TGTTTCCATG GATAATAAGT GATTACTCCA GCCCAGAGCT AGATTTGTCA
    AATCCTGCAA CCTTCCGAGA TCTTAGTAAG CCAGTGGGAG CCCTAAATGC GGAGCGACTG
    GAGAGACTGC TGACTCGATA CCAGGAAATG CCTGAGCCAA GATTCATGTA TGGGAGTCAC
    TACTCTTCTC CAGGCTATGT GCTCTTTTAC CTCGTTAGGA TCGCACCAGA GTATATGCTG
    TGTCTACAAA ATGGAAGATT TGATAATGCA GATAGGATGT TTAACAGTAT TGCAGAAACG
    TGGAAAAACT GTTTGGATGG TGCAACGGAT TTTAAAGAGT TGATTCCAGA ATTCTATGAT
    GAAGACGTCA GCTTTTTGGT CAATAGTCTG AAGCTGGATC TGGGAAAGAG ACAAGGCGGG
    CAGATGGTTG ATGACGTGGA CCTTCCAGCC TGGGCCTCAA GTCCCCAAGA CTTCCTGCAG
    AAGAACAAGG ATGCCTTGGA AAGCGGTTAC GTGTCTGAGC ACCTTCATGA GTGGATTGAT
    CTGATATTTG GCTATAAGCA AAAAGGGAGT GAGGCCATAG GGGCCCATAA CGTGTTTCAT
    CCCCTGACCT ATGAAGGTGG TGTAGACTTG AACAGCATTG AGGATCCTGA TGAAAAAGTA
    GCCATGCTGA CCCAAATTTT GGAATTTGGG CAGACACCAA AACAGCTGTT TGTGACACCA
    CATCCTCGAA GGATCACCCC CAAGTTTAAA AGTTTGTCCC AAGCCTCCAG CTACAACGCT
    TCTCTGACAG ACTCCCCAGT TTCTCCAGGG GAGGAGTCGT TTGAAGACCT GACTGAGGAA
    AGCAGAACTC TGGCCTGGAG TAATATCGCC AAGCTGCAGC TACATGAGCA GTACAAGATC
    CACAAAGAGG CGGTTACAGG GATAGCAGTG TCTTGCAATG GCTCTTCAGT CTTTACCACC
    TCCCAAGATT CCACTTTGAA GATGTTTTCT AAGGAATCCA AAATGCTACA AAGAAGTATA
    TCATTTTCCA ATATGGCTTT ATCCTCTTGT TTACTGTTGC CGGGAGACAC CACTGTCATA
    AGTTCTTCTT GGGATAACAA CGTCTATTTT TATTCCATAG CATTTGGAAG ACGCCAAGAT
    ACACTGATGG GACATGACGA TGCTGTCAGT AAGATCTGTT GGCATAACGA CAGGCTGTAC
    TCTGGATCTT GGGACTCTAC TGTGAAGGTG TGGTCCGGTG TTCCTGCAGA AATGCCAGGA
    ACAAAAAGAC ACCAGTTTGA CTTACTGGCT GAGCTTGAAC ATGATGTCAG CGTGAGTCCA
    GCAGCAGAGC TCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011248246.1
    CDS630..3044
    Translation

    Target ORF information:

    RefSeq Version XM_011249944.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus neutral sphingomyelinase (N-SMase) activation associated factor (Nsmaf), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011249944.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGCGTTCA CCCGGAAGAG GCAGCGCGAG CAGCAGCTGC AGCTCTACTC CAAGGAGAGA 
    TGCGGGTTTG TCTGTGGGAG CCGGGATCCG ACGGTCACGT TGTTGCTGAG TGGATCCAGC
    GAAGCAGGCT ATGCCAGGAA ATGCAGGTTT TCCTTACTGC TTCTCAACTT GGAGGAGTAC
    TACTTTGAGC AGCACACAGC GTTCCATGTC CAGCACCAGG GCAGCCAGGA AGAAAGGAAA
    ATCAGAGGCT CCTTAAAGAT ATGTTCAAAA TCAGTGATTT TTGAACCAGA TGCAATATCT
    CAGCCCATTC TTAAGATTCC TTTGAGAGAC TGTTTAAAAA TAGGAAAACA TGGAGAAAAT
    GGAGCCAATA AACACTTTGC AAAGGCAAAA TCATGGGGTA TTTCACTCAT TTTCAGTCAG
    ATATACTTTA TTAAGGAGCA CAACATTGTG GCACCGTATA AGATAGAGCG GGGAAAAATG
    GAGTATGTCT TTGAACTGGA AGTTTCTGGG AAGGTGGAAG ATGTTGTGGA GACATTGCTA
    CAGCTGCACA GAGCATCCTG CCTTGATAAA CTTGGTGACC AAATGGCCAT GATAACAGCT
    ATCTTGCAGT CACGTCTGGC TAGGACATCA TTTGACAAAA ACAGGTTCCA AAGTGTTTCT
    GAAAAGCTGC ACATGGAGTG CAAAGCAGAG ATGGTAACAC CTCTAGTGAC GAACCCTGGG
    CATGTGTGCA TTACAGACAC CAGCCTGTAT TTCCAGCCTC TCAATGGGTA CCCGAAACCT
    GTGGTCCAGA TAACACTGCA GGATGTCCGG CGCATTTACA AAAGGAGGCA CGGCCTCATG
    CCTTTGGGCT TGGAAGTGTT TTGCACAGAT GACGACCTGT GTTCCGACAT ATACCTAAAG
    TTCTATGAGC CTCAAGACAG AGATGATCTC TATTTTTATA TCGCCACGTA CCTAGAACAC
    CACGCTGCAG AACACACTGC GGAGAGCTAC ATGCTGCAGT GGCAGCGAGG GCACCTTTCC
    AACTACCAGT ACCTGCTCCA CCTCAACAAC CTGGCCGACC GCAGCTGCAA TGACCTGTCC
    CAGTACCCTG TGTTTCCATG GATAATAAGT GATTACTCCA GCCCAGAGCT AGATTTGTCA
    AATCCTGCAA CCTTCCGAGA TCTTAGTAAG CCAGTGGGAG CCCTAAATGC GGAGCGACTG
    GAGAGACTGC TGACTCGATA CCAGGAAATG CCTGAGCCAA GATTCATGTA TGGGAGTCAC
    TACTCTTCTC CAGGCTATGT GCTCTTTTAC CTCGTTAGGA TCGCACCAGA GTATATGCTG
    TGTCTACAAA ATGGAAGATT TGATAATGCA GATAGGATGT TTAACAGTAT TGCAGAAACG
    TGGAAAAACT GTTTGGATGG TGCAACGGAT TTTAAAGAGT TGATTCCAGA ATTCTATGAT
    GAAGACGTCA GCTTTTTGGT CAATAGTCTG AAGCTGGATC TGGGAAAGAG ACAAGGCGGG
    CAGATGGTTG ATGACGTGGA CCTTCCAGCC TGGGCCTCAA GTCCCCAAGA CTTCCTGCAG
    AAGAACAAGG ATGCCTTGGA AAGCGGTTAC GTGTCTGAGC ACCTTCATGA GTGGATTGAT
    CTGATATTTG GCTATAAGCA AAAAGGGAGT GAGGCCATAG GGGCCCATAA CGTGTTTCAT
    CCCCTGACCT ATGAAGGTGG TGTAGACTTG AACAGCATTG AGGATCCTGA TGAAAAAGTA
    GCCATGCTGA CCCAAATTTT GGAATTTGGG CAGACACCAA AACAGCTGTT TGTGACACCA
    CATCCTCGAA GGATCACCCC CAAGTTTAAA AGTTTGTCCC AAGCCTCCAG CTACAACGCT
    TCTCTGACAG ACTCCCCAGT TTCTCCAGGG GAGGAGTCGT TTGAAGACCT GACTGAGGAA
    AGCAGAACTC TGGCCTGGAG TAATATCGCC AAGCTGCAGC TACATGAGCA GTACAAGATC
    CACAAAGAGG CGGTTACAGG GATAGCAGTG TCTTGCAATG GCTCTTCAGT CTTTACCACC
    TCCCAAGATT CCACTTTGAA GATGTTTTCT AAGGAATCCA AAATGCTACA AAGAAGTATA
    TCATTTTCCA ATATGGCTTT ATCCTCTTGT TTACTGTTGC CGGGAGACAC CACTGTCATA
    AGTTCTTCTT GGGATAACAA CGTCTATTTT TATTCCATAG CATTTGGAAG ACGCCAAGAT
    ACACTGATGG GACATGACGA TGCTGTCAGT AAGATCTGTT GGCATAACGA CAGGCTGTAC
    TCTGGATCTT GGGACTCTAC TGTGAAGGTG TGGTCCGGTG TTCCTGCAGA AATGCCAGGA
    ACAAAAAGAC ACCAGTTTGA CTTACTGGCT GAGCTTGAAC ATGATGTCAG CGTGAGTCCA
    GCAGCAGAGC TCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu32752
    Clone ID Related Accession (Same CDS sequence) XM_006537662.4 , XM_006537662.3
    Accession Version XM_006537662.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2871bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product protein FAN isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006537662.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGCGTTCA CCCGGAAGAG GCAGCGCGAG CAGCAGCTGC AGCTCTACTC CAAGGAGAGA 
    TGCGGGTTTG TCTGTGGGAG CCGGGATCCG ACGGTCACGT TGTTGCTGAG TGGATCCAGC
    GAAGCAGGCT ATGCCAGGAA ATGCAGGTTT TCCTTACTGC TTCTCAACTT GGAGGAGTAC
    TACTTTGAGC AGCACACAGC GTTCCATGTC CAGCACCAGG GCAGCCAGGA AGAAAGGAAA
    ATCAGAGGCT CCTTAAAGAT ATGTTCAAAA TCAGTGATTT TTGAACCAGA TGCAATATCT
    CAGCCCATTC TTAAGATTCC TTTGAGAGAC TGTTTAAAAA TAGGAAAACA TGGAGAAAAT
    GGAGCCAATA AACACTTTGC AAAGGCAAAA TCATGGGGTA TTTCACTCAT TTTCAGTCAG
    ATATACTTTA TTAAGGAGCA CAACATTGTG GCACCGTATA AGATAGAGCG GGGAAAAATG
    GAGTATGTCT TTGAACTGGA AGTTTCTGGG AAGGTGGAAG ATGTTGTGGA GACATTGCTA
    CAGCTGCACA GAGCATCCTG CCTTGATAAA CTTGGTGACC AAATGGCCAT GATAACAGCT
    ATCTTGCAGT CACGTCTGGC TAGGACATCA TTTGACAAAA ACAGGTTCCA AAGTGTTTCT
    GAAAAGCTGC ACATGGAGTG CAAAGCAGAG ATGGTAACAC CTCTAGTGAC GAACCCTGGG
    CATGTGTGCA TTACAGACAC CAGCCTGTAT TTCCAGCCTC TCAATGGGTA CCCGAAACCT
    GTGGTCCAGA TAACACTGCA GGATGTCCGG CGCATTTACA AAAGGAGGCA CGGCCTCATG
    CCTTTGGGCT TGGAAGTGTT TTGCACAGAT GACGACCTGT GTTCCGACAT ATACCTAAAG
    TTCTATGAGC CTCAAGACAG AGATGATCTC TATTTTTATA TCGCCACGTA CCTAGAACAC
    CACGCTGCAG AACACACTGC GGAGAGCTAC ATGCTGCAGT GGCAGCGAGG GCACCTTTCC
    AACTACCAGT ACCTGCTCCA CCTCAACAAC CTGGCCGACC GCAGCTGCAA TGACCTGTCC
    CAGTACCCTG TGTTTCCATG GATAATAAGT GATTACTCCA GCCCAGAGCT AGATTTGTCA
    AATCCTGCAA CCTTCCGAGA TCTTAGTAAG CCAGTGGGAG CCCTAAATGC GGAGCGACTG
    GAGAGACTGC TGACTCGATA CCAGGAAATG CCTGAGCCAA GATTCATGTA TGGGAGTCAC
    TACTCTTCTC CAGGCTATGT GCTCTTTTAC CTCGTTAGGA TCGCACCAGA GTATATGCTG
    TGTCTACAAA ATGGAAGATT TGATAATGCA GATAGGATGT TTAACAGTAT TGCAGAAACG
    TGGAAAAACT GTTTGGATGG TGCAACGGAT TTTAAAGAGT TGATTCCAGA ATTCTATGAT
    GAAGACGTCA GCTTTTTGGT CAATAGTCTG AAGCTGGATC TGGGAAAGAG ACAAGGCGGG
    CAGATGGTTG ATGACGTGGA CCTTCCAGCC TGGGCCTCAA GTCCCCAAGA CTTCCTGCAG
    AAGAACAAGG ATGCCTTGGA AAGCGGTTAC GTGTCTGAGC ACCTTCATGA GTGGATTGAT
    CTGATATTTG GCTATAAGCA AAAAGGGAGT GAGGCCATAG GGGCCCATAA CGTGTTTCAT
    CCCCTGACCT ATGAAGGTGG TGTAGACTTG AACAGCATTG AGGATCCTGA TGAAAAAGTA
    GCCATGCTGA CCCAAATTTT GGAATTTGGG CAGACACCAA AACAGCTGTT TGTGACACCA
    CATCCTCGAA GGATCACCCC CAAGTTTAAA AGTTTGTCCC AAGCCTCCAG CTACAACGCT
    TCTCTGACAG ACTCCCCAGT TTCTCCAGGG GAGGAGTCGT TTGAAGACCT GACTGAGGAA
    AGCAGAACTC TGGCCTGGAG TAATATCGCC AAGCTGCAGC TACATGAGCA GTACAAGATC
    CACAAAGAGG CGGTTACAGG GATAGCAGTG TCTTGCAATG GCTCTTCAGT CTTTACCACC
    TCCCAAGATT CCACTTTGAA GATGTTTTCT AAGGAATCCA AAATGCTACA AAGAAGTATA
    TCATTTTCCA ATATGGCTTT ATCCTCTTGT TTACTGTTGC CGGGAGACAC CACTGTCATA
    AGTTCTTCTT GGGATAACAA CGTCTATTTT TATTCCATAG CATTTGGAAG ACGCCAAGAT
    ACACTGATGG GACATGACGA TGCTGTCAGT AAGATCTGTT GGCATAACGA CAGGCTGTAC
    TCTGGATCTT GGGACTCTAC TGTGAAGGTG TGGTCCGGTG TTCCTGCAGA AATGCCAGGA
    ACAAAAAGAC ACCAGTTTGA CTTACTGGCT GAGCTTGAAC ATGATGTCAG CATGACTTAT
    CTACTGGAGA AGGTCAATAC TATCAATTTA AATGCTGTGA GCACACTGTT AGTTTCTGGC
    ACCAAAGAAG GCATGGTGAA TATTTGGGAC CTCACTACAG CCACTTTATT ACACCAGACT
    TCATGCCATT CAGGGACAGT ATGTGATGCT GCTTTTAGCC CAGATAGTCG CCACATCCTC
    AGCACGGGCG TAGATGGATG TCTCAATGTG ATTGATGTGC AGACGGGCAT GCTCATCTCC
    TCCATGGCCT CAGAGGAGCC GCAGAGGTGC TTTGTCTGGG ATGGAAATTC TGTCTTATCT
    GGAAGTCGGT CTGGTGAGCT GCTTGTTTGG GACCTCCTTG GAGCAAAAGT CAGTGAGCGG
    ATTCAGGGCC ACACTGGTGC TGTGACATGC ATGTGGATGA ACGAACAGTG TAGCAGTATT
    ATCACCGGAG GGGAAGACAG ACAAATTATG TTCTGGAAAT TGCAGTATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006537725.1
    CDS798..3668
    Translation

    Target ORF information:

    RefSeq Version XM_006537662.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus neutral sphingomyelinase (N-SMase) activation associated factor (Nsmaf), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006537662.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGCGTTCA CCCGGAAGAG GCAGCGCGAG CAGCAGCTGC AGCTCTACTC CAAGGAGAGA 
    TGCGGGTTTG TCTGTGGGAG CCGGGATCCG ACGGTCACGT TGTTGCTGAG TGGATCCAGC
    GAAGCAGGCT ATGCCAGGAA ATGCAGGTTT TCCTTACTGC TTCTCAACTT GGAGGAGTAC
    TACTTTGAGC AGCACACAGC GTTCCATGTC CAGCACCAGG GCAGCCAGGA AGAAAGGAAA
    ATCAGAGGCT CCTTAAAGAT ATGTTCAAAA TCAGTGATTT TTGAACCAGA TGCAATATCT
    CAGCCCATTC TTAAGATTCC TTTGAGAGAC TGTTTAAAAA TAGGAAAACA TGGAGAAAAT
    GGAGCCAATA AACACTTTGC AAAGGCAAAA TCATGGGGTA TTTCACTCAT TTTCAGTCAG
    ATATACTTTA TTAAGGAGCA CAACATTGTG GCACCGTATA AGATAGAGCG GGGAAAAATG
    GAGTATGTCT TTGAACTGGA AGTTTCTGGG AAGGTGGAAG ATGTTGTGGA GACATTGCTA
    CAGCTGCACA GAGCATCCTG CCTTGATAAA CTTGGTGACC AAATGGCCAT GATAACAGCT
    ATCTTGCAGT CACGTCTGGC TAGGACATCA TTTGACAAAA ACAGGTTCCA AAGTGTTTCT
    GAAAAGCTGC ACATGGAGTG CAAAGCAGAG ATGGTAACAC CTCTAGTGAC GAACCCTGGG
    CATGTGTGCA TTACAGACAC CAGCCTGTAT TTCCAGCCTC TCAATGGGTA CCCGAAACCT
    GTGGTCCAGA TAACACTGCA GGATGTCCGG CGCATTTACA AAAGGAGGCA CGGCCTCATG
    CCTTTGGGCT TGGAAGTGTT TTGCACAGAT GACGACCTGT GTTCCGACAT ATACCTAAAG
    TTCTATGAGC CTCAAGACAG AGATGATCTC TATTTTTATA TCGCCACGTA CCTAGAACAC
    CACGCTGCAG AACACACTGC GGAGAGCTAC ATGCTGCAGT GGCAGCGAGG GCACCTTTCC
    AACTACCAGT ACCTGCTCCA CCTCAACAAC CTGGCCGACC GCAGCTGCAA TGACCTGTCC
    CAGTACCCTG TGTTTCCATG GATAATAAGT GATTACTCCA GCCCAGAGCT AGATTTGTCA
    AATCCTGCAA CCTTCCGAGA TCTTAGTAAG CCAGTGGGAG CCCTAAATGC GGAGCGACTG
    GAGAGACTGC TGACTCGATA CCAGGAAATG CCTGAGCCAA GATTCATGTA TGGGAGTCAC
    TACTCTTCTC CAGGCTATGT GCTCTTTTAC CTCGTTAGGA TCGCACCAGA GTATATGCTG
    TGTCTACAAA ATGGAAGATT TGATAATGCA GATAGGATGT TTAACAGTAT TGCAGAAACG
    TGGAAAAACT GTTTGGATGG TGCAACGGAT TTTAAAGAGT TGATTCCAGA ATTCTATGAT
    GAAGACGTCA GCTTTTTGGT CAATAGTCTG AAGCTGGATC TGGGAAAGAG ACAAGGCGGG
    CAGATGGTTG ATGACGTGGA CCTTCCAGCC TGGGCCTCAA GTCCCCAAGA CTTCCTGCAG
    AAGAACAAGG ATGCCTTGGA AAGCGGTTAC GTGTCTGAGC ACCTTCATGA GTGGATTGAT
    CTGATATTTG GCTATAAGCA AAAAGGGAGT GAGGCCATAG GGGCCCATAA CGTGTTTCAT
    CCCCTGACCT ATGAAGGTGG TGTAGACTTG AACAGCATTG AGGATCCTGA TGAAAAAGTA
    GCCATGCTGA CCCAAATTTT GGAATTTGGG CAGACACCAA AACAGCTGTT TGTGACACCA
    CATCCTCGAA GGATCACCCC CAAGTTTAAA AGTTTGTCCC AAGCCTCCAG CTACAACGCT
    TCTCTGACAG ACTCCCCAGT TTCTCCAGGG GAGGAGTCGT TTGAAGACCT GACTGAGGAA
    AGCAGAACTC TGGCCTGGAG TAATATCGCC AAGCTGCAGC TACATGAGCA GTACAAGATC
    CACAAAGAGG CGGTTACAGG GATAGCAGTG TCTTGCAATG GCTCTTCAGT CTTTACCACC
    TCCCAAGATT CCACTTTGAA GATGTTTTCT AAGGAATCCA AAATGCTACA AAGAAGTATA
    TCATTTTCCA ATATGGCTTT ATCCTCTTGT TTACTGTTGC CGGGAGACAC CACTGTCATA
    AGTTCTTCTT GGGATAACAA CGTCTATTTT TATTCCATAG CATTTGGAAG ACGCCAAGAT
    ACACTGATGG GACATGACGA TGCTGTCAGT AAGATCTGTT GGCATAACGA CAGGCTGTAC
    TCTGGATCTT GGGACTCTAC TGTGAAGGTG TGGTCCGGTG TTCCTGCAGA AATGCCAGGA
    ACAAAAAGAC ACCAGTTTGA CTTACTGGCT GAGCTTGAAC ATGATGTCAG CATGACTTAT
    CTACTGGAGA AGGTCAATAC TATCAATTTA AATGCTGTGA GCACACTGTT AGTTTCTGGC
    ACCAAAGAAG GCATGGTGAA TATTTGGGAC CTCACTACAG CCACTTTATT ACACCAGACT
    TCATGCCATT CAGGGACAGT ATGTGATGCT GCTTTTAGCC CAGATAGTCG CCACATCCTC
    AGCACGGGCG TAGATGGATG TCTCAATGTG ATTGATGTGC AGACGGGCAT GCTCATCTCC
    TCCATGGCCT CAGAGGAGCC GCAGAGGTGC TTTGTCTGGG ATGGAAATTC TGTCTTATCT
    GGAAGTCGGT CTGGTGAGCT GCTTGTTTGG GACCTCCTTG GAGCAAAAGT CAGTGAGCGG
    ATTCAGGGCC ACACTGGTGC TGTGACATGC ATGTGGATGA ACGAACAGTG TAGCAGTATT
    ATCACCGGAG GGGAAGACAG ACAAATTATG TTCTGGAAAT TGCAGTATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu32753
    Clone ID Related Accession (Same CDS sequence) XM_006537663.2 , XM_006537663.3
    Accession Version XM_006537663.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2850bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product protein FAN isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006537663.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGCGTTCA CCCGGAAGAG GCAGCGCGAG CAGCAGCTGC AGCTCTACTC CAAGGAGAGA 
    TGCGGGTTTG TCTGTGGGAG CCGGGATCCG ACGGTCACGT TGTTGCTGAG TGGATCCAGC
    GAAGCAGGCT ATGCCAGGAA ATGCAGGTTT TCCTTACTGC TTCTCAACTT GGAGGAGTAC
    TACTTTGAGC AGCACACAGC GTTCCATGTC CAGCACCAGG GCAGCCAGGA AGAAAGGAAA
    ATCAGAGGCT CCTTAAAGAT ATGTTCAAAA TCAGTGATTT TTGAACCAGA TGCAATATCT
    CAGCCCATTC TTAAGATTCC TTTGAGAGAC TGTTTAAAAA TAGGAAAACA TGGAGAAAAT
    GGAGCCAATA AACACTTTGC AAAGGCAAAA TCATGGGGTA TTTCACTCAT TTTCAGTCAG
    ATATACTTTA TTAAGGAGCA CAACATTGTG GCACCGTATA AGATAGAGCG GGGAAAAATG
    GAGTATGTCT TTGAACTGGA AGTTTCTGGG AAGGTGGAAG ATGTTGTGGA GACATTGCTA
    CAGCTGCACA GAGCATCCTG CCTTGATAAA CTTGGTGACC AAATGGCCAT GATAACAGCT
    ATCTTGCAGT CACGTCTGGC TAGGACATCA TTTGACAAAA ACAGGTTCCA AAGTGTTTCT
    GAAAAGCTGC ACATGGAGTG CAAAGCAGAG ATGGTAACAC CTCTAGTGAC GAACCCTGGG
    CATGTGTGCA TTACAGACAC CAGCCTGTAT TTCCAGCCTC TCAATGGGTA CCCGAAACCT
    GTGGTCCAGA TAACACTGCA GGATGTCCGG CGCATTTACA AAAGGAGGCA CGGCCTCATG
    CCTTTGGGCT TGGAAGTGTT TTGCACAGAT GACGACCTGT GTTCCGACAT ATACCTAAAG
    TTCTATGAGC CTCAAGACAG AGATGATCTC TATTTTTATA TCGCCACGTA CCTAGAACAC
    CACGCTGCAG AACACACTGC GGAGAGCTAC ATGCTGCAGT GGCAGCGAGG GCACCTTTCC
    AACTACCAGT ACCTGCTCCA CCTCAACAAC CTGGCCGACC GCAGCTGCAA TGACCTGTCC
    CAGTACCCTG TGTTTCCATG GATAATAAGT GATTACTCCA GCCCAGAGCT AGATTTGTCA
    AATCCTGCAA CCTTCCGAGA TCTTAGTAAG CCAGTGGGAG CCCTAAATGC GGAGCGACTG
    GAGAGACTGC TGACTCGATA CCAGGAAATG CCTGAGCCAA GATTCATGTA TGGGAGTCAC
    TACTCTTCTC CAGGCTATGT GCTCTTTTAC CTCGTTAGGA TCGCACCAGA GTATATGCTG
    TGTCTACAAA ATGGAAGATT TGATAATGCA GATAGGATGT TTAACAGTAT TGCAGAAACG
    TGGAAAAACT GTTTGGATGG TGCAACGGAT TTTAAAGAGT TGATTCCAGA ATTCTATGAT
    GAAGACGTCA GCTTTTTGGT CAATAGTCTG AAGCTGGATC TGGGAAAGAG ACAAGGCGGG
    CAGATGGTTG ATGACGTGGA CCTTCCAGCC TGGGCCTCAA GTCCCCAAGA CTTCCTGCAG
    AAGAACAAGG ATGCCTTGGA AAGCGGTTAC GTGTCTGAGC ACCTTCATGA GTGGATTGAT
    CTGATATTTG GCTATAAGCA AAAAGGGAGT GAGGCCATAG GGGCCCATAA CGTGTTTCAT
    CCCCTGACCT ATGAAGGTGG TGTAGACTTG AACAGCATTG AGGATCCTGA TGAAAAAGTA
    GCCATGCTGA CCCAAATTTT GGAATTTGGG CAGACACCAA AACAGCTGTT TGTGACACCA
    CATCCTCGAA GGATCACCCC CAAGTTTAAA AGTTTGTCCC AAGCCTCCAG CTACAACGCT
    TCTCTGACAG ACTCCCCAGT TTCTCCAGGG GAGGAGTCGT TTGAAGACCT GACTGAGGAA
    AGCAGAACTC TGGCCTGGAG TAATATCGCC AAGCTGCAGC TACATGAGCA GTACAAGATC
    CACAAAGAGG CGGTTACAGG GATAGCAGTG TCTTGCAATG GCTCTTCAGT CTTTACCACC
    TCCCAAGATT CCACTTTGAA GATGTTTTCT AAGGAATCCA AAATGCTACA AAGAAGTATA
    TCATTTTCCA ATATGGCTTT ATCCTCTTGT TTACTGTTGC CGGGAGACAC CACTGTCATA
    AGTTCTTCTT GGGATAACAA CGTCTATTTT TATTCCATAG CATTTGGAAG ACGCCAAGAT
    ACACTGATGG GACATGACGA TGCTGTCAGT AAGATCTGTT GGCATAACGA CAGGCTGTAC
    TCTGGATCTT GGGACTCTAC TGTGAAGGTG TGGTCCGGTG TTCCTGCAGA AATGCCAGGA
    ACAAAAAGAC ACCAGTTTGA CTTACTGGCT GAGCTTGAAC ATGATGTCAG CGTCAATACT
    ATCAATTTAA ATGCTGTGAG CACACTGTTA GTTTCTGGCA CCAAAGAAGG CATGGTGAAT
    ATTTGGGACC TCACTACAGC CACTTTATTA CACCAGACTT CATGCCATTC AGGGACAGTA
    TGTGATGCTG CTTTTAGCCC AGATAGTCGC CACATCCTCA GCACGGGCGT AGATGGATGT
    CTCAATGTGA TTGATGTGCA GACGGGCATG CTCATCTCCT CCATGGCCTC AGAGGAGCCG
    CAGAGGTGCT TTGTCTGGGA TGGAAATTCT GTCTTATCTG GAAGTCGGTC TGGTGAGCTG
    CTTGTTTGGG ACCTCCTTGG AGCAAAAGTC AGTGAGCGGA TTCAGGGCCA CACTGGTGCT
    GTGACATGCA TGTGGATGAA CGAACAGTGT AGCAGTATTA TCACCGGAGG GGAAGACAGA
    CAAATTATGT TCTGGAAATT GCAGTATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006537726.1
    CDS798..3647
    Translation

    Target ORF information:

    RefSeq Version XM_006537663.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus neutral sphingomyelinase (N-SMase) activation associated factor (Nsmaf), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006537663.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGCGTTCA CCCGGAAGAG GCAGCGCGAG CAGCAGCTGC AGCTCTACTC CAAGGAGAGA 
    TGCGGGTTTG TCTGTGGGAG CCGGGATCCG ACGGTCACGT TGTTGCTGAG TGGATCCAGC
    GAAGCAGGCT ATGCCAGGAA ATGCAGGTTT TCCTTACTGC TTCTCAACTT GGAGGAGTAC
    TACTTTGAGC AGCACACAGC GTTCCATGTC CAGCACCAGG GCAGCCAGGA AGAAAGGAAA
    ATCAGAGGCT CCTTAAAGAT ATGTTCAAAA TCAGTGATTT TTGAACCAGA TGCAATATCT
    CAGCCCATTC TTAAGATTCC TTTGAGAGAC TGTTTAAAAA TAGGAAAACA TGGAGAAAAT
    GGAGCCAATA AACACTTTGC AAAGGCAAAA TCATGGGGTA TTTCACTCAT TTTCAGTCAG
    ATATACTTTA TTAAGGAGCA CAACATTGTG GCACCGTATA AGATAGAGCG GGGAAAAATG
    GAGTATGTCT TTGAACTGGA AGTTTCTGGG AAGGTGGAAG ATGTTGTGGA GACATTGCTA
    CAGCTGCACA GAGCATCCTG CCTTGATAAA CTTGGTGACC AAATGGCCAT GATAACAGCT
    ATCTTGCAGT CACGTCTGGC TAGGACATCA TTTGACAAAA ACAGGTTCCA AAGTGTTTCT
    GAAAAGCTGC ACATGGAGTG CAAAGCAGAG ATGGTAACAC CTCTAGTGAC GAACCCTGGG
    CATGTGTGCA TTACAGACAC CAGCCTGTAT TTCCAGCCTC TCAATGGGTA CCCGAAACCT
    GTGGTCCAGA TAACACTGCA GGATGTCCGG CGCATTTACA AAAGGAGGCA CGGCCTCATG
    CCTTTGGGCT TGGAAGTGTT TTGCACAGAT GACGACCTGT GTTCCGACAT ATACCTAAAG
    TTCTATGAGC CTCAAGACAG AGATGATCTC TATTTTTATA TCGCCACGTA CCTAGAACAC
    CACGCTGCAG AACACACTGC GGAGAGCTAC ATGCTGCAGT GGCAGCGAGG GCACCTTTCC
    AACTACCAGT ACCTGCTCCA CCTCAACAAC CTGGCCGACC GCAGCTGCAA TGACCTGTCC
    CAGTACCCTG TGTTTCCATG GATAATAAGT GATTACTCCA GCCCAGAGCT AGATTTGTCA
    AATCCTGCAA CCTTCCGAGA TCTTAGTAAG CCAGTGGGAG CCCTAAATGC GGAGCGACTG
    GAGAGACTGC TGACTCGATA CCAGGAAATG CCTGAGCCAA GATTCATGTA TGGGAGTCAC
    TACTCTTCTC CAGGCTATGT GCTCTTTTAC CTCGTTAGGA TCGCACCAGA GTATATGCTG
    TGTCTACAAA ATGGAAGATT TGATAATGCA GATAGGATGT TTAACAGTAT TGCAGAAACG
    TGGAAAAACT GTTTGGATGG TGCAACGGAT TTTAAAGAGT TGATTCCAGA ATTCTATGAT
    GAAGACGTCA GCTTTTTGGT CAATAGTCTG AAGCTGGATC TGGGAAAGAG ACAAGGCGGG
    CAGATGGTTG ATGACGTGGA CCTTCCAGCC TGGGCCTCAA GTCCCCAAGA CTTCCTGCAG
    AAGAACAAGG ATGCCTTGGA AAGCGGTTAC GTGTCTGAGC ACCTTCATGA GTGGATTGAT
    CTGATATTTG GCTATAAGCA AAAAGGGAGT GAGGCCATAG GGGCCCATAA CGTGTTTCAT
    CCCCTGACCT ATGAAGGTGG TGTAGACTTG AACAGCATTG AGGATCCTGA TGAAAAAGTA
    GCCATGCTGA CCCAAATTTT GGAATTTGGG CAGACACCAA AACAGCTGTT TGTGACACCA
    CATCCTCGAA GGATCACCCC CAAGTTTAAA AGTTTGTCCC AAGCCTCCAG CTACAACGCT
    TCTCTGACAG ACTCCCCAGT TTCTCCAGGG GAGGAGTCGT TTGAAGACCT GACTGAGGAA
    AGCAGAACTC TGGCCTGGAG TAATATCGCC AAGCTGCAGC TACATGAGCA GTACAAGATC
    CACAAAGAGG CGGTTACAGG GATAGCAGTG TCTTGCAATG GCTCTTCAGT CTTTACCACC
    TCCCAAGATT CCACTTTGAA GATGTTTTCT AAGGAATCCA AAATGCTACA AAGAAGTATA
    TCATTTTCCA ATATGGCTTT ATCCTCTTGT TTACTGTTGC CGGGAGACAC CACTGTCATA
    AGTTCTTCTT GGGATAACAA CGTCTATTTT TATTCCATAG CATTTGGAAG ACGCCAAGAT
    ACACTGATGG GACATGACGA TGCTGTCAGT AAGATCTGTT GGCATAACGA CAGGCTGTAC
    TCTGGATCTT GGGACTCTAC TGTGAAGGTG TGGTCCGGTG TTCCTGCAGA AATGCCAGGA
    ACAAAAAGAC ACCAGTTTGA CTTACTGGCT GAGCTTGAAC ATGATGTCAG CGTCAATACT
    ATCAATTTAA ATGCTGTGAG CACACTGTTA GTTTCTGGCA CCAAAGAAGG CATGGTGAAT
    ATTTGGGACC TCACTACAGC CACTTTATTA CACCAGACTT CATGCCATTC AGGGACAGTA
    TGTGATGCTG CTTTTAGCCC AGATAGTCGC CACATCCTCA GCACGGGCGT AGATGGATGT
    CTCAATGTGA TTGATGTGCA GACGGGCATG CTCATCTCCT CCATGGCCTC AGAGGAGCCG
    CAGAGGTGCT TTGTCTGGGA TGGAAATTCT GTCTTATCTG GAAGTCGGTC TGGTGAGCTG
    CTTGTTTGGG ACCTCCTTGG AGCAAAAGTC AGTGAGCGGA TTCAGGGCCA CACTGGTGCT
    GTGACATGCA TGTGGATGAA CGAACAGTGT AGCAGTATTA TCACCGGAGG GGAAGACAGA
    CAAATTATGT TCTGGAAATT GCAGTATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu32754
    Clone ID Related Accession (Same CDS sequence) XM_006537664.2 , XM_006537664.3
    Accession Version XM_006537664.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2784bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product protein FAN isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006537664.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCGTTCA CCCGGAAGAG GCAGCGCGAG CAGCAGCTGC AGCTCTACTC CAAGGAGAGG 
    TTTTCCTTAC TGCTTCTCAA CTTGGAGGAG TACTACTTTG AGCAGCACAC AGCGTTCCAT
    GTCCAGCACC AGGGCAGCCA GGAAGAAAGG AAAATCAGAG GCTCCTTAAA GATATGTTCA
    AAATCAGTGA TTTTTGAACC AGATGCAATA TCTCAGCCCA TTCTTAAGAT TCCTTTGAGA
    GACTGTTTAA AAATAGGAAA ACATGGAGAA AATGGAGCCA ATAAACACTT TGCAAAGGCA
    AAATCATGGG GTATTTCACT CATTTTCAGT CAGATATACT TTATTAAGGA GCACAACATT
    GTGGCACCGT ATAAGATAGA GCGGGGAAAA ATGGAGTATG TCTTTGAACT GGAAGTTTCT
    GGGAAGGTGG AAGATGTTGT GGAGACATTG CTACAGCTGC ACAGAGCATC CTGCCTTGAT
    AAACTTGGTG ACCAAATGGC CATGATAACA GCTATCTTGC AGTCACGTCT GGCTAGGACA
    TCATTTGACA AAAACAGGTT CCAAAGTGTT TCTGAAAAGC TGCACATGGA GTGCAAAGCA
    GAGATGGTAA CACCTCTAGT GACGAACCCT GGGCATGTGT GCATTACAGA CACCAGCCTG
    TATTTCCAGC CTCTCAATGG GTACCCGAAA CCTGTGGTCC AGATAACACT GCAGGATGTC
    CGGCGCATTT ACAAAAGGAG GCACGGCCTC ATGCCTTTGG GCTTGGAAGT GTTTTGCACA
    GATGACGACC TGTGTTCCGA CATATACCTA AAGTTCTATG AGCCTCAAGA CAGAGATGAT
    CTCTATTTTT ATATCGCCAC GTACCTAGAA CACCACGCTG CAGAACACAC TGCGGAGAGC
    TACATGCTGC AGTGGCAGCG AGGGCACCTT TCCAACTACC AGTACCTGCT CCACCTCAAC
    AACCTGGCCG ACCGCAGCTG CAATGACCTG TCCCAGTACC CTGTGTTTCC ATGGATAATA
    AGTGATTACT CCAGCCCAGA GCTAGATTTG TCAAATCCTG CAACCTTCCG AGATCTTAGT
    AAGCCAGTGG GAGCCCTAAA TGCGGAGCGA CTGGAGAGAC TGCTGACTCG ATACCAGGAA
    ATGCCTGAGC CAAGATTCAT GTATGGGAGT CACTACTCTT CTCCAGGCTA TGTGCTCTTT
    TACCTCGTTA GGATCGCACC AGAGTATATG CTGTGTCTAC AAAATGGAAG ATTTGATAAT
    GCAGATAGGA TGTTTAACAG TATTGCAGAA ACGTGGAAAA ACTGTTTGGA TGGTGCAACG
    GATTTTAAAG AGTTGATTCC AGAATTCTAT GATGAAGACG TCAGCTTTTT GGTCAATAGT
    CTGAAGCTGG ATCTGGGAAA GAGACAAGGC GGGCAGATGG TTGATGACGT GGACCTTCCA
    GCCTGGGCCT CAAGTCCCCA AGACTTCCTG CAGAAGAACA AGGATGCCTT GGAAAGCGGT
    TACGTGTCTG AGCACCTTCA TGAGTGGATT GATCTGATAT TTGGCTATAA GCAAAAAGGG
    AGTGAGGCCA TAGGGGCCCA TAACGTGTTT CATCCCCTGA CCTATGAAGG TGGTGTAGAC
    TTGAACAGCA TTGAGGATCC TGATGAAAAA GTAGCCATGC TGACCCAAAT TTTGGAATTT
    GGGCAGACAC CAAAACAGCT GTTTGTGACA CCACATCCTC GAAGGATCAC CCCCAAGTTT
    AAAAGTTTGT CCCAAGCCTC CAGCTACAAC GCTTCTCTGA CAGACTCCCC AGTTTCTCCA
    GGGGAGGAGT CGTTTGAAGA CCTGACTGAG GAAAGCAGAA CTCTGGCCTG GAGTAATATC
    GCCAAGCTGC AGCTACATGA GCAGTACAAG ATCCACAAAG AGGCGGTTAC AGGGATAGCA
    GTGTCTTGCA ATGGCTCTTC AGTCTTTACC ACCTCCCAAG ATTCCACTTT GAAGATGTTT
    TCTAAGGAAT CCAAAATGCT ACAAAGAAGT ATATCATTTT CCAATATGGC TTTATCCTCT
    TGTTTACTGT TGCCGGGAGA CACCACTGTC ATAAGTTCTT CTTGGGATAA CAACGTCTAT
    TTTTATTCCA TAGCATTTGG AAGACGCCAA GATACACTGA TGGGACATGA CGATGCTGTC
    AGTAAGATCT GTTGGCATAA CGACAGGCTG TACTCTGGAT CTTGGGACTC TACTGTGAAG
    GTGTGGTCCG GTGTTCCTGC AGAAATGCCA GGAACAAAAA GACACCAGTT TGACTTACTG
    GCTGAGCTTG AACATGATGT CAGCATGACT TATCTACTGG AGAAGGTCAA TACTATCAAT
    TTAAATGCTG TGAGCACACT GTTAGTTTCT GGCACCAAAG AAGGCATGGT GAATATTTGG
    GACCTCACTA CAGCCACTTT ATTACACCAG ACTTCATGCC ATTCAGGGAC AGTATGTGAT
    GCTGCTTTTA GCCCAGATAG TCGCCACATC CTCAGCACGG GCGTAGATGG ATGTCTCAAT
    GTGATTGATG TGCAGACGGG CATGCTCATC TCCTCCATGG CCTCAGAGGA GCCGCAGAGG
    TGCTTTGTCT GGGATGGAAA TTCTGTCTTA TCTGGAAGTC GGTCTGGTGA GCTGCTTGTT
    TGGGACCTCC TTGGAGCAAA AGTCAGTGAG CGGATTCAGG GCCACACTGG TGCTGTGACA
    TGCATGTGGA TGAACGAACA GTGTAGCAGT ATTATCACCG GAGGGGAAGA CAGACAAATT
    ATGTTCTGGA AATTGCAGTA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006537727.1
    CDS798..3581
    Translation

    Target ORF information:

    RefSeq Version XM_006537664.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus neutral sphingomyelinase (N-SMase) activation associated factor (Nsmaf), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006537664.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCGTTCA CCCGGAAGAG GCAGCGCGAG CAGCAGCTGC AGCTCTACTC CAAGGAGAGG 
    TTTTCCTTAC TGCTTCTCAA CTTGGAGGAG TACTACTTTG AGCAGCACAC AGCGTTCCAT
    GTCCAGCACC AGGGCAGCCA GGAAGAAAGG AAAATCAGAG GCTCCTTAAA GATATGTTCA
    AAATCAGTGA TTTTTGAACC AGATGCAATA TCTCAGCCCA TTCTTAAGAT TCCTTTGAGA
    GACTGTTTAA AAATAGGAAA ACATGGAGAA AATGGAGCCA ATAAACACTT TGCAAAGGCA
    AAATCATGGG GTATTTCACT CATTTTCAGT CAGATATACT TTATTAAGGA GCACAACATT
    GTGGCACCGT ATAAGATAGA GCGGGGAAAA ATGGAGTATG TCTTTGAACT GGAAGTTTCT
    GGGAAGGTGG AAGATGTTGT GGAGACATTG CTACAGCTGC ACAGAGCATC CTGCCTTGAT
    AAACTTGGTG ACCAAATGGC CATGATAACA GCTATCTTGC AGTCACGTCT GGCTAGGACA
    TCATTTGACA AAAACAGGTT CCAAAGTGTT TCTGAAAAGC TGCACATGGA GTGCAAAGCA
    GAGATGGTAA CACCTCTAGT GACGAACCCT GGGCATGTGT GCATTACAGA CACCAGCCTG
    TATTTCCAGC CTCTCAATGG GTACCCGAAA CCTGTGGTCC AGATAACACT GCAGGATGTC
    CGGCGCATTT ACAAAAGGAG GCACGGCCTC ATGCCTTTGG GCTTGGAAGT GTTTTGCACA
    GATGACGACC TGTGTTCCGA CATATACCTA AAGTTCTATG AGCCTCAAGA CAGAGATGAT
    CTCTATTTTT ATATCGCCAC GTACCTAGAA CACCACGCTG CAGAACACAC TGCGGAGAGC
    TACATGCTGC AGTGGCAGCG AGGGCACCTT TCCAACTACC AGTACCTGCT CCACCTCAAC
    AACCTGGCCG ACCGCAGCTG CAATGACCTG TCCCAGTACC CTGTGTTTCC ATGGATAATA
    AGTGATTACT CCAGCCCAGA GCTAGATTTG TCAAATCCTG CAACCTTCCG AGATCTTAGT
    AAGCCAGTGG GAGCCCTAAA TGCGGAGCGA CTGGAGAGAC TGCTGACTCG ATACCAGGAA
    ATGCCTGAGC CAAGATTCAT GTATGGGAGT CACTACTCTT CTCCAGGCTA TGTGCTCTTT
    TACCTCGTTA GGATCGCACC AGAGTATATG CTGTGTCTAC AAAATGGAAG ATTTGATAAT
    GCAGATAGGA TGTTTAACAG TATTGCAGAA ACGTGGAAAA ACTGTTTGGA TGGTGCAACG
    GATTTTAAAG AGTTGATTCC AGAATTCTAT GATGAAGACG TCAGCTTTTT GGTCAATAGT
    CTGAAGCTGG ATCTGGGAAA GAGACAAGGC GGGCAGATGG TTGATGACGT GGACCTTCCA
    GCCTGGGCCT CAAGTCCCCA AGACTTCCTG CAGAAGAACA AGGATGCCTT GGAAAGCGGT
    TACGTGTCTG AGCACCTTCA TGAGTGGATT GATCTGATAT TTGGCTATAA GCAAAAAGGG
    AGTGAGGCCA TAGGGGCCCA TAACGTGTTT CATCCCCTGA CCTATGAAGG TGGTGTAGAC
    TTGAACAGCA TTGAGGATCC TGATGAAAAA GTAGCCATGC TGACCCAAAT TTTGGAATTT
    GGGCAGACAC CAAAACAGCT GTTTGTGACA CCACATCCTC GAAGGATCAC CCCCAAGTTT
    AAAAGTTTGT CCCAAGCCTC CAGCTACAAC GCTTCTCTGA CAGACTCCCC AGTTTCTCCA
    GGGGAGGAGT CGTTTGAAGA CCTGACTGAG GAAAGCAGAA CTCTGGCCTG GAGTAATATC
    GCCAAGCTGC AGCTACATGA GCAGTACAAG ATCCACAAAG AGGCGGTTAC AGGGATAGCA
    GTGTCTTGCA ATGGCTCTTC AGTCTTTACC ACCTCCCAAG ATTCCACTTT GAAGATGTTT
    TCTAAGGAAT CCAAAATGCT ACAAAGAAGT ATATCATTTT CCAATATGGC TTTATCCTCT
    TGTTTACTGT TGCCGGGAGA CACCACTGTC ATAAGTTCTT CTTGGGATAA CAACGTCTAT
    TTTTATTCCA TAGCATTTGG AAGACGCCAA GATACACTGA TGGGACATGA CGATGCTGTC
    AGTAAGATCT GTTGGCATAA CGACAGGCTG TACTCTGGAT CTTGGGACTC TACTGTGAAG
    GTGTGGTCCG GTGTTCCTGC AGAAATGCCA GGAACAAAAA GACACCAGTT TGACTTACTG
    GCTGAGCTTG AACATGATGT CAGCATGACT TATCTACTGG AGAAGGTCAA TACTATCAAT
    TTAAATGCTG TGAGCACACT GTTAGTTTCT GGCACCAAAG AAGGCATGGT GAATATTTGG
    GACCTCACTA CAGCCACTTT ATTACACCAG ACTTCATGCC ATTCAGGGAC AGTATGTGAT
    GCTGCTTTTA GCCCAGATAG TCGCCACATC CTCAGCACGG GCGTAGATGG ATGTCTCAAT
    GTGATTGATG TGCAGACGGG CATGCTCATC TCCTCCATGG CCTCAGAGGA GCCGCAGAGG
    TGCTTTGTCT GGGATGGAAA TTCTGTCTTA TCTGGAAGTC GGTCTGGTGA GCTGCTTGTT
    TGGGACCTCC TTGGAGCAAA AGTCAGTGAG CGGATTCAGG GCCACACTGG TGCTGTGACA
    TGCATGTGGA TGAACGAACA GTGTAGCAGT ATTATCACCG GAGGGGAAGA CAGACAAATT
    ATGTTCTGGA AATTGCAGTA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu68335
    Clone ID Related Accession (Same CDS sequence) XM_011249943.2 , XM_011249943.3
    Accession Version XM_011249943.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2469bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product protein FAN isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011249943.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGCGTTCA CCCGGAAGAG GCAGCGCGAG CAGCAGCTGC AGCTCTACTC CAAGGAGAGA 
    TGCGGGTTTG TCTGTGGGAG CCGGGATCCG ACGGTCACGT TGTTGCTGAG TGGATCCAGC
    GAAGCAGGCT ATGCCAGGAA ATGCAGGTTT TCCTTACTGC TTCTCAACTT GGAGGAGTAC
    TACTTTGAGC AGCACACAGC GTTCCATGTC CAGCACCAGG GCAGCCAGGA AGAAAGGAAA
    ATCAGAGGCT CCTTAAAGAT ATGTTCAAAA TCAGTGATTT TTGAACCAGA TGCAATATCT
    CAGCCCATTC TTAAGATTCC TTTGAGAGAC TGTTTAAAAA TAGGAAAACA TGGAGAAAAT
    GGAGCCAATA AACACTTTGC AAAGGCAAAA TCATGGGGTA TTTCACTCAT TTTCAGTCAG
    ATATACTTTA TTAAGGAGCA CAACATTGTG GCACCGTATA AGATAGAGCG GGGAAAAATG
    GAGTATGTCT TTGAACTGGA AGTTTCTGGG AAGGTGGAAG ATGTTGTGGA GACATTGCTA
    CAGCTGCACA GAGCATCCTG CCTTGATAAA CTTGGTGACC AAATGGCCAT GATAACAGCT
    ATCTTGCAGT CACGTCTGGC TAGGACATCA TTTGACAAAA ACAGGTTCCA AAGTGTTTCT
    GAAAAGCTGC ACATGGAGTG CAAAGCAGAG ATGGTAACAC CTCTAGTGAC GAACCCTGGG
    CATGTGTGCA TTACAGACAC CAGCCTGTAT TTCCAGCCTC TCAATGGGTA CCCGAAACCT
    GTGGTCCAGA TAACACTGCA GGATGTCCGG CGCATTTACA AAAGGAGGCA CGGCCTCATG
    CCTTTGGGCT TGGAAGTGTT TTGCACAGAT GACGACCTGT GTTCCGACAT ATACCTAAAG
    TTCTATGAGC CTCAAGACAG AGATGATCTC TATTTTTATA TCGCCACGTA CCTAGAACAC
    CACGCTGCAG AACACACTGC GGAGAGCTAC ATGCTGCAGT GGCAGCGAGG GCACCTTTCC
    AACTACCAGT ACCTGCTCCA CCTCAACAAC CTGGCCGACC GCAGCTGCAA TGACCTGTCC
    CAGTACCCTG TGTTTCCATG GATAATAAGT GATTACTCCA GCCCAGAGCT AGATTTGTCA
    AATCCTGCAA CCTTCCGAGA TCTTAGTAAG CCAGTGGGAG CCCTAAATGC GGAGCGACTG
    GAGAGACTGC TGACTCGATA CCAGGAAATG CCTGAGCCAA GATTCATGTA TGGGAGTCAC
    TACTCTTCTC CAGGCTATGT GCTCTTTTAC CTCGTTAGGA TCGCACCAGA GTATATGCTG
    TGTCTACAAA ATGGAAGATT TGATAATGCA GATAGGATGT TTAACAGTAT TGCAGAAACG
    TGGAAAAACT GTTTGGATGG TGCAACGGAT TTTAAAGAGT TGATTCCAGA ATTCTATGAT
    GAAGACGTCA GCTTTTTGGT CAATAGTCTG AAGCTGGATC TGGGAAAGAG ACAAGGCGGG
    CAGATGGTTG ATGACGTGGA CCTTCCAGCC TGGGCCTCAA GTCCCCAAGA CTTCCTGCAG
    AAGAACAAGG ATGCCTTGGA AAGCGGTTAC GTGTCTGAGC ACCTTCATGA GTGGATTGAT
    CTGATATTTG GCTATAAGCA AAAAGGGAGT GAGGCCATAG GGGCCCATAA CGTGTTTCAT
    CCCCTGACCT ATGAAGGTGG TGTAGACTTG AACAGCATTG AGGATCCTGA TGAAAAAGTA
    GCCATGCTGA CCCAAATTTT GGAATTTGGG CAGACACCAA AACAGCTGTT TGTGACACCA
    CATCCTCGAA GGATCACCCC CAAGTTTAAA AGTTTGTCCC AAGCCTCCAG CTACAACGCT
    TCTCTGACAG ACTCCCCAGT TTCTCCAGGG GAGGAGTCGT TTGAAGACCT GACTGAGGAA
    AGCAGAACTC TGGCCTGGAG TAATATCGCC AAGCTGCAGC TACATGAGCA GTACAAGATC
    CACAAAGAGG CGGTTACAGG GATAGCAGTG TCTTGCAATG GCTCTTCAGT CTTTACCACC
    TCCCAAGATT CCACTTTGAA GATGTTTTCT AAGGAATCCA AAATGCTACA AAGAAGTATA
    TCATTTTCCA ATATGGCTTT ATCCTCTTGT TTACTGTTGC CGGGAGACAC CACTGTCATA
    AGTTCTTCTT GGGATAACAA CGTCTATTTT TATTCCATAG CATTTGGAAG ACGCCAAGAT
    ACACTGATGG GACATGACGA TGCTGTCAGT AAGATCTGTT GGCATAACGA CAGGCTGTAC
    TCTGGATCTT GGGACTCTAC TGTGAAGGTG TGGTCCGGTG TTCCTGCAGA AATGCCAGGA
    ACAAAAAGAC ACCAGTTTGA CTTACTGGCT GAGCTTGAAC ATGATGTCAG CATGACTTAT
    CTACTGGAGA AGAAAAGAAA TGCTGTACTT CGTGTCCCTC CTCTCCTCCA GTCAGAGTTT
    TGCTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011248245.1
    CDS798..3266
    Translation

    Target ORF information:

    RefSeq Version XM_011249943.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus neutral sphingomyelinase (N-SMase) activation associated factor (Nsmaf), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011249943.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGCGTTCA CCCGGAAGAG GCAGCGCGAG CAGCAGCTGC AGCTCTACTC CAAGGAGAGA 
    TGCGGGTTTG TCTGTGGGAG CCGGGATCCG ACGGTCACGT TGTTGCTGAG TGGATCCAGC
    GAAGCAGGCT ATGCCAGGAA ATGCAGGTTT TCCTTACTGC TTCTCAACTT GGAGGAGTAC
    TACTTTGAGC AGCACACAGC GTTCCATGTC CAGCACCAGG GCAGCCAGGA AGAAAGGAAA
    ATCAGAGGCT CCTTAAAGAT ATGTTCAAAA TCAGTGATTT TTGAACCAGA TGCAATATCT
    CAGCCCATTC TTAAGATTCC TTTGAGAGAC TGTTTAAAAA TAGGAAAACA TGGAGAAAAT
    GGAGCCAATA AACACTTTGC AAAGGCAAAA TCATGGGGTA TTTCACTCAT TTTCAGTCAG
    ATATACTTTA TTAAGGAGCA CAACATTGTG GCACCGTATA AGATAGAGCG GGGAAAAATG
    GAGTATGTCT TTGAACTGGA AGTTTCTGGG AAGGTGGAAG ATGTTGTGGA GACATTGCTA
    CAGCTGCACA GAGCATCCTG CCTTGATAAA CTTGGTGACC AAATGGCCAT GATAACAGCT
    ATCTTGCAGT CACGTCTGGC TAGGACATCA TTTGACAAAA ACAGGTTCCA AAGTGTTTCT
    GAAAAGCTGC ACATGGAGTG CAAAGCAGAG ATGGTAACAC CTCTAGTGAC GAACCCTGGG
    CATGTGTGCA TTACAGACAC CAGCCTGTAT TTCCAGCCTC TCAATGGGTA CCCGAAACCT
    GTGGTCCAGA TAACACTGCA GGATGTCCGG CGCATTTACA AAAGGAGGCA CGGCCTCATG
    CCTTTGGGCT TGGAAGTGTT TTGCACAGAT GACGACCTGT GTTCCGACAT ATACCTAAAG
    TTCTATGAGC CTCAAGACAG AGATGATCTC TATTTTTATA TCGCCACGTA CCTAGAACAC
    CACGCTGCAG AACACACTGC GGAGAGCTAC ATGCTGCAGT GGCAGCGAGG GCACCTTTCC
    AACTACCAGT ACCTGCTCCA CCTCAACAAC CTGGCCGACC GCAGCTGCAA TGACCTGTCC
    CAGTACCCTG TGTTTCCATG GATAATAAGT GATTACTCCA GCCCAGAGCT AGATTTGTCA
    AATCCTGCAA CCTTCCGAGA TCTTAGTAAG CCAGTGGGAG CCCTAAATGC GGAGCGACTG
    GAGAGACTGC TGACTCGATA CCAGGAAATG CCTGAGCCAA GATTCATGTA TGGGAGTCAC
    TACTCTTCTC CAGGCTATGT GCTCTTTTAC CTCGTTAGGA TCGCACCAGA GTATATGCTG
    TGTCTACAAA ATGGAAGATT TGATAATGCA GATAGGATGT TTAACAGTAT TGCAGAAACG
    TGGAAAAACT GTTTGGATGG TGCAACGGAT TTTAAAGAGT TGATTCCAGA ATTCTATGAT
    GAAGACGTCA GCTTTTTGGT CAATAGTCTG AAGCTGGATC TGGGAAAGAG ACAAGGCGGG
    CAGATGGTTG ATGACGTGGA CCTTCCAGCC TGGGCCTCAA GTCCCCAAGA CTTCCTGCAG
    AAGAACAAGG ATGCCTTGGA AAGCGGTTAC GTGTCTGAGC ACCTTCATGA GTGGATTGAT
    CTGATATTTG GCTATAAGCA AAAAGGGAGT GAGGCCATAG GGGCCCATAA CGTGTTTCAT
    CCCCTGACCT ATGAAGGTGG TGTAGACTTG AACAGCATTG AGGATCCTGA TGAAAAAGTA
    GCCATGCTGA CCCAAATTTT GGAATTTGGG CAGACACCAA AACAGCTGTT TGTGACACCA
    CATCCTCGAA GGATCACCCC CAAGTTTAAA AGTTTGTCCC AAGCCTCCAG CTACAACGCT
    TCTCTGACAG ACTCCCCAGT TTCTCCAGGG GAGGAGTCGT TTGAAGACCT GACTGAGGAA
    AGCAGAACTC TGGCCTGGAG TAATATCGCC AAGCTGCAGC TACATGAGCA GTACAAGATC
    CACAAAGAGG CGGTTACAGG GATAGCAGTG TCTTGCAATG GCTCTTCAGT CTTTACCACC
    TCCCAAGATT CCACTTTGAA GATGTTTTCT AAGGAATCCA AAATGCTACA AAGAAGTATA
    TCATTTTCCA ATATGGCTTT ATCCTCTTGT TTACTGTTGC CGGGAGACAC CACTGTCATA
    AGTTCTTCTT GGGATAACAA CGTCTATTTT TATTCCATAG CATTTGGAAG ACGCCAAGAT
    ACACTGATGG GACATGACGA TGCTGTCAGT AAGATCTGTT GGCATAACGA CAGGCTGTAC
    TCTGGATCTT GGGACTCTAC TGTGAAGGTG TGGTCCGGTG TTCCTGCAGA AATGCCAGGA
    ACAAAAAGAC ACCAGTTTGA CTTACTGGCT GAGCTTGAAC ATGATGTCAG CATGACTTAT
    CTACTGGAGA AGAAAAGAAA TGCTGTACTT CGTGTCCCTC CTCTCCTCCA GTCAGAGTTT
    TGCTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu68336
    Clone ID Related Accession (Same CDS sequence) XM_011249944.2 , XM_011249944.3
    Accession Version XM_011249944.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2415bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product protein FAN isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011249944.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGCGTTCA CCCGGAAGAG GCAGCGCGAG CAGCAGCTGC AGCTCTACTC CAAGGAGAGA 
    TGCGGGTTTG TCTGTGGGAG CCGGGATCCG ACGGTCACGT TGTTGCTGAG TGGATCCAGC
    GAAGCAGGCT ATGCCAGGAA ATGCAGGTTT TCCTTACTGC TTCTCAACTT GGAGGAGTAC
    TACTTTGAGC AGCACACAGC GTTCCATGTC CAGCACCAGG GCAGCCAGGA AGAAAGGAAA
    ATCAGAGGCT CCTTAAAGAT ATGTTCAAAA TCAGTGATTT TTGAACCAGA TGCAATATCT
    CAGCCCATTC TTAAGATTCC TTTGAGAGAC TGTTTAAAAA TAGGAAAACA TGGAGAAAAT
    GGAGCCAATA AACACTTTGC AAAGGCAAAA TCATGGGGTA TTTCACTCAT TTTCAGTCAG
    ATATACTTTA TTAAGGAGCA CAACATTGTG GCACCGTATA AGATAGAGCG GGGAAAAATG
    GAGTATGTCT TTGAACTGGA AGTTTCTGGG AAGGTGGAAG ATGTTGTGGA GACATTGCTA
    CAGCTGCACA GAGCATCCTG CCTTGATAAA CTTGGTGACC AAATGGCCAT GATAACAGCT
    ATCTTGCAGT CACGTCTGGC TAGGACATCA TTTGACAAAA ACAGGTTCCA AAGTGTTTCT
    GAAAAGCTGC ACATGGAGTG CAAAGCAGAG ATGGTAACAC CTCTAGTGAC GAACCCTGGG
    CATGTGTGCA TTACAGACAC CAGCCTGTAT TTCCAGCCTC TCAATGGGTA CCCGAAACCT
    GTGGTCCAGA TAACACTGCA GGATGTCCGG CGCATTTACA AAAGGAGGCA CGGCCTCATG
    CCTTTGGGCT TGGAAGTGTT TTGCACAGAT GACGACCTGT GTTCCGACAT ATACCTAAAG
    TTCTATGAGC CTCAAGACAG AGATGATCTC TATTTTTATA TCGCCACGTA CCTAGAACAC
    CACGCTGCAG AACACACTGC GGAGAGCTAC ATGCTGCAGT GGCAGCGAGG GCACCTTTCC
    AACTACCAGT ACCTGCTCCA CCTCAACAAC CTGGCCGACC GCAGCTGCAA TGACCTGTCC
    CAGTACCCTG TGTTTCCATG GATAATAAGT GATTACTCCA GCCCAGAGCT AGATTTGTCA
    AATCCTGCAA CCTTCCGAGA TCTTAGTAAG CCAGTGGGAG CCCTAAATGC GGAGCGACTG
    GAGAGACTGC TGACTCGATA CCAGGAAATG CCTGAGCCAA GATTCATGTA TGGGAGTCAC
    TACTCTTCTC CAGGCTATGT GCTCTTTTAC CTCGTTAGGA TCGCACCAGA GTATATGCTG
    TGTCTACAAA ATGGAAGATT TGATAATGCA GATAGGATGT TTAACAGTAT TGCAGAAACG
    TGGAAAAACT GTTTGGATGG TGCAACGGAT TTTAAAGAGT TGATTCCAGA ATTCTATGAT
    GAAGACGTCA GCTTTTTGGT CAATAGTCTG AAGCTGGATC TGGGAAAGAG ACAAGGCGGG
    CAGATGGTTG ATGACGTGGA CCTTCCAGCC TGGGCCTCAA GTCCCCAAGA CTTCCTGCAG
    AAGAACAAGG ATGCCTTGGA AAGCGGTTAC GTGTCTGAGC ACCTTCATGA GTGGATTGAT
    CTGATATTTG GCTATAAGCA AAAAGGGAGT GAGGCCATAG GGGCCCATAA CGTGTTTCAT
    CCCCTGACCT ATGAAGGTGG TGTAGACTTG AACAGCATTG AGGATCCTGA TGAAAAAGTA
    GCCATGCTGA CCCAAATTTT GGAATTTGGG CAGACACCAA AACAGCTGTT TGTGACACCA
    CATCCTCGAA GGATCACCCC CAAGTTTAAA AGTTTGTCCC AAGCCTCCAG CTACAACGCT
    TCTCTGACAG ACTCCCCAGT TTCTCCAGGG GAGGAGTCGT TTGAAGACCT GACTGAGGAA
    AGCAGAACTC TGGCCTGGAG TAATATCGCC AAGCTGCAGC TACATGAGCA GTACAAGATC
    CACAAAGAGG CGGTTACAGG GATAGCAGTG TCTTGCAATG GCTCTTCAGT CTTTACCACC
    TCCCAAGATT CCACTTTGAA GATGTTTTCT AAGGAATCCA AAATGCTACA AAGAAGTATA
    TCATTTTCCA ATATGGCTTT ATCCTCTTGT TTACTGTTGC CGGGAGACAC CACTGTCATA
    AGTTCTTCTT GGGATAACAA CGTCTATTTT TATTCCATAG CATTTGGAAG ACGCCAAGAT
    ACACTGATGG GACATGACGA TGCTGTCAGT AAGATCTGTT GGCATAACGA CAGGCTGTAC
    TCTGGATCTT GGGACTCTAC TGTGAAGGTG TGGTCCGGTG TTCCTGCAGA AATGCCAGGA
    ACAAAAAGAC ACCAGTTTGA CTTACTGGCT GAGCTTGAAC ATGATGTCAG CGTGAGTCCA
    GCAGCAGAGC TCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011248246.1
    CDS798..3212
    Translation

    Target ORF information:

    RefSeq Version XM_011249944.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus neutral sphingomyelinase (N-SMase) activation associated factor (Nsmaf), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011249944.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGCGTTCA CCCGGAAGAG GCAGCGCGAG CAGCAGCTGC AGCTCTACTC CAAGGAGAGA 
    TGCGGGTTTG TCTGTGGGAG CCGGGATCCG ACGGTCACGT TGTTGCTGAG TGGATCCAGC
    GAAGCAGGCT ATGCCAGGAA ATGCAGGTTT TCCTTACTGC TTCTCAACTT GGAGGAGTAC
    TACTTTGAGC AGCACACAGC GTTCCATGTC CAGCACCAGG GCAGCCAGGA AGAAAGGAAA
    ATCAGAGGCT CCTTAAAGAT ATGTTCAAAA TCAGTGATTT TTGAACCAGA TGCAATATCT
    CAGCCCATTC TTAAGATTCC TTTGAGAGAC TGTTTAAAAA TAGGAAAACA TGGAGAAAAT
    GGAGCCAATA AACACTTTGC AAAGGCAAAA TCATGGGGTA TTTCACTCAT TTTCAGTCAG
    ATATACTTTA TTAAGGAGCA CAACATTGTG GCACCGTATA AGATAGAGCG GGGAAAAATG
    GAGTATGTCT TTGAACTGGA AGTTTCTGGG AAGGTGGAAG ATGTTGTGGA GACATTGCTA
    CAGCTGCACA GAGCATCCTG CCTTGATAAA CTTGGTGACC AAATGGCCAT GATAACAGCT
    ATCTTGCAGT CACGTCTGGC TAGGACATCA TTTGACAAAA ACAGGTTCCA AAGTGTTTCT
    GAAAAGCTGC ACATGGAGTG CAAAGCAGAG ATGGTAACAC CTCTAGTGAC GAACCCTGGG
    CATGTGTGCA TTACAGACAC CAGCCTGTAT TTCCAGCCTC TCAATGGGTA CCCGAAACCT
    GTGGTCCAGA TAACACTGCA GGATGTCCGG CGCATTTACA AAAGGAGGCA CGGCCTCATG
    CCTTTGGGCT TGGAAGTGTT TTGCACAGAT GACGACCTGT GTTCCGACAT ATACCTAAAG
    TTCTATGAGC CTCAAGACAG AGATGATCTC TATTTTTATA TCGCCACGTA CCTAGAACAC
    CACGCTGCAG AACACACTGC GGAGAGCTAC ATGCTGCAGT GGCAGCGAGG GCACCTTTCC
    AACTACCAGT ACCTGCTCCA CCTCAACAAC CTGGCCGACC GCAGCTGCAA TGACCTGTCC
    CAGTACCCTG TGTTTCCATG GATAATAAGT GATTACTCCA GCCCAGAGCT AGATTTGTCA
    AATCCTGCAA CCTTCCGAGA TCTTAGTAAG CCAGTGGGAG CCCTAAATGC GGAGCGACTG
    GAGAGACTGC TGACTCGATA CCAGGAAATG CCTGAGCCAA GATTCATGTA TGGGAGTCAC
    TACTCTTCTC CAGGCTATGT GCTCTTTTAC CTCGTTAGGA TCGCACCAGA GTATATGCTG
    TGTCTACAAA ATGGAAGATT TGATAATGCA GATAGGATGT TTAACAGTAT TGCAGAAACG
    TGGAAAAACT GTTTGGATGG TGCAACGGAT TTTAAAGAGT TGATTCCAGA ATTCTATGAT
    GAAGACGTCA GCTTTTTGGT CAATAGTCTG AAGCTGGATC TGGGAAAGAG ACAAGGCGGG
    CAGATGGTTG ATGACGTGGA CCTTCCAGCC TGGGCCTCAA GTCCCCAAGA CTTCCTGCAG
    AAGAACAAGG ATGCCTTGGA AAGCGGTTAC GTGTCTGAGC ACCTTCATGA GTGGATTGAT
    CTGATATTTG GCTATAAGCA AAAAGGGAGT GAGGCCATAG GGGCCCATAA CGTGTTTCAT
    CCCCTGACCT ATGAAGGTGG TGTAGACTTG AACAGCATTG AGGATCCTGA TGAAAAAGTA
    GCCATGCTGA CCCAAATTTT GGAATTTGGG CAGACACCAA AACAGCTGTT TGTGACACCA
    CATCCTCGAA GGATCACCCC CAAGTTTAAA AGTTTGTCCC AAGCCTCCAG CTACAACGCT
    TCTCTGACAG ACTCCCCAGT TTCTCCAGGG GAGGAGTCGT TTGAAGACCT GACTGAGGAA
    AGCAGAACTC TGGCCTGGAG TAATATCGCC AAGCTGCAGC TACATGAGCA GTACAAGATC
    CACAAAGAGG CGGTTACAGG GATAGCAGTG TCTTGCAATG GCTCTTCAGT CTTTACCACC
    TCCCAAGATT CCACTTTGAA GATGTTTTCT AAGGAATCCA AAATGCTACA AAGAAGTATA
    TCATTTTCCA ATATGGCTTT ATCCTCTTGT TTACTGTTGC CGGGAGACAC CACTGTCATA
    AGTTCTTCTT GGGATAACAA CGTCTATTTT TATTCCATAG CATTTGGAAG ACGCCAAGAT
    ACACTGATGG GACATGACGA TGCTGTCAGT AAGATCTGTT GGCATAACGA CAGGCTGTAC
    TCTGGATCTT GGGACTCTAC TGTGAAGGTG TGGTCCGGTG TTCCTGCAGA AATGCCAGGA
    ACAAAAAGAC ACCAGTTTGA CTTACTGGCT GAGCTTGAAC ATGATGTCAG CGTGAGTCCA
    GCAGCAGAGC TCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.