NSMAF cDNA ORF clone, Canis lupus familiaris(Dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	NSMAF
Entrez Gene ID	477892
Full Name	neutral sphingomyelinase activation associated factor
General protein information	Preferred Names neutral sphingomyelinase activation associated factor Names protein FAN
Gene Type	protein-coding
Organism	Canis lupus familiaris(Dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_022412291.1	XP_022267999.1	protein FAN
XM_014109189.1	XP_013964664.1	protein FAN

Pathways from BioSystems

Homologies

Bos taurus (cattle)	NSMAF	XP_002692713.2
Homo sapiens (human)	NSMAF	NP_003571.2
Pan troglodytes (chimpanzee)	NSMAF	XP_001156895.1
Mus musculus (house mouse)	Nsmaf	NP_035075.2
Rattus norvegicus (Norway rat)	Nsmaf	NP_852054.1
Canis lupus familiaris (dog)	NSMAF	XP_005638205.1
Danio rerio (zebrafish)	nsmaf	XP_003199050.1
	NSMAF	XP_001088417.1
Gallus gallus (chicken)	NSMAF	XP_003640887.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000429	XP_310670.5
Xenopus tropicalis (tropical clawed frog)	nsmaf	NP_001121464.1

Gene Ontology

The following NSMAF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NSMAF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCe44097	XM_014109189.1 Latest version!	Canis lupus familiaris neutral sphingomyelinase (N-SMase) activation associated factor (NSMAF), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NSMAF ( XM_014109189.1 ) cDNA ORF clone, Canis lupus familiaris(Dog) -> XP_013964664.1 Canis lupus familiaris neutral sphingomyelinase (N-SMase) activation associated factor (NSMAF), mRNA.

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CloneID	OCe44097
Clone ID Related Accession (Same CDS sequence)	XM_014109189.1
Accession Version	XM_014109189.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2793bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	protein FAN
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726112.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 3% of CDS bases ##RefSeq-Attributes-END##

ATGAGGCAGG ACATGGGAGC AGAGGGGGTG TGCAGCCTGG AAGGGGCGGT GGGGAAGCCT 
GGTGGTCAGT CAGCAGGAGC AAAGGATGAA AAAACTCTAT TTTCCTTGCT CCTGCTTAAC 
TTGGAGGAAT ACTACTTTGA ACAACATACC GCTAATCATA TTCAGAACAA GGGTGGTCAG 
AATGAAAGGA AAATCAGAGG CTCCTTAAAA ATATGTTCAA AATCAGTTAT TTTTGAACCA 
GATGCCATAT CTCAGCCCAT CATTAAGATT CCTTTGAGAG ATTGTATAAA AATAGGAAAA 
CATGGAGAAA ATGGATCCAA CAGACACTTT GCAAAGACAA AATCCGGGGA GATCTCACTC 
ATTTTCAGTC AGGTGTATTT CATTAAAGAA CACAACATTG TTGCACCATA TAAAATAGAA 
AGGGGCAAAA TGGAGTATGT CTTTGAATTG GACGTTTCAG GGAAAGTGGA AGATGTGGTG 
GAGACGTTGC TTCAGCTTCA CAGAGCATCC TGCCTCGACA AACTAGGAGA CCAGACTGCT 
ATGATAACAG CTATTTTGCA GTCACGTTTG GCTAGGACAT CATTTGACAA AAACAGATTC 
CAAAGTGTTT CTGAAAAGCT GCACATGGAA TGCAAAGCAG AAATGGTGAC ACCGCTGGTG 
ACGAATCCAG GACATGTGTG CATCACTGAC ACAAACCTGT ATTTTCAGCC TCTCAATGGG 
TACCCGAAAC CTGTGGTCCA GATAACACTC CAAGATGTCC GCCGCATTTA CAAAAGGAGG 
CACGGCCTCA TGCCTTTGGG CTTGGAAGTC TTTTGCACAG AAGATGATCT CTGTTCTGAC 
ATCTACTTAA AGTTCTATGA ACCTCAAGAT AGAGATGACC TCTATTTTTA TATTGCCACA 
TATCTCGAGC ACCACGTGGC CGAGCGCACG GCCGAGAGCT ACACGCTGCA GTGGCAGCGC 
GGCCACCTCT CCAACTACCA GTACCTGCTC CACCTCAACA ACCTGGCGGA CCGCAGCTGC 
AACGACCTGT CGCAGTACCC CGTGTTCCCC TGGATCATCA GCGACTACTG CAGTGCCGAG 
CTTGATTTGT CAAATCCAGG AACCTTCCGG GATCTTAGCA GGCCAGTAGG GGCCCTAAAC 
AAGGAAAGGC TGGAGAGACT CCTGGCCCGC TACCAGGAAA TGCCAGAGCC AAAGTTCATG 
TACGGCAGTC ACTACTCATC CCCGGGTTAT GTGCTATTTT ATCTTGTTAG GATCGCGCCC 
GAGTACATGC TGTGCCTGCA GAATGGAAGA TTTGATAATG CAGACCGAAT GTTCAACAGT 
ATTGGAGAAA CTTGGAAAAA CTGTTTGGAT GGTGCAACAG ATTTTAAAGA GTTGATTCCA 
GAATTCTATG GTGATGATGT CAGCTTTTTA GTCAATAATC TGAAGTTGGA TCTGGGAAAG 
AGACAAGGAG GACAGATGGT TGATGATGTG GAACTTCCCC CCTGGGCACG GAGTCCTGAG 
GATTTTCTCC AGAAGAGCAA AGACGCGCTG GAAAGCAATT ATGTGTCCGA GCACCTCCAC 
GAGTGGATCG ATCTGATATT TGGCTACAAG CAAAAAGGGA GCGATGCCGT TGGGGCCCAT 
AATGTATTTC ATCCCCTGAC CTATGAAGGA GGCGTAGACC TTAACAGCAT TGAGGACCCT 
GATGAGAAAG TAGCCATGCT GACCCAAATC TTGGAGTTTG GCCAGACGCC GAAACAACTC 
TTCGTGACGC CACATCCACG ACGGATCACC CCAAAGTTTA AAAATTTCTC CCAAACCTAT 
AGTCATAATG CTCCCATGGC AGATTCTCCA GGTGAGGAGT CCTTTGAAGA CCTGACGGAA 
GAAAGCAAAA CCCTGGCCTG GAATAACATC GCCAAGCTGC AGTTACACGA CCAGTATAAG 
ATCCACAAAG AGGCAGTTAC TGGGATAGCG GTCTCTTGCA ACGGGTCCTC TGTCTTTACA 
ACCTCACAAG ATTCCACCTT GAAGATGTTC TCTAAGGAAT CCAAAATGCT ACAAAGAAGC 
ATATCATTTT CAAATATGGC TTTATCATCT TGTTTACTTT TGCCAGGAGA TGCCACTGTC 
ATAAGTTCTT CATGGGATAA TAACATCTAT TTTTATTCTA TAGCATTTGC AAGACGCCAG 
GACACGTTAA TGGGACACGA TGATGCCGTT AGTAAGATCT GTTGGCATGA CAACCGACTG 
TATTCTGCAT CGTGGGACTC CACAGTGAAG GTGTGGTCTG GTGTTCCTGC AGAAATGACC 
TGCACCAGGA GACAGCGGTT TGACATGCTG GCCGAGCTGG AACACGACGT CAGTGTAGAT 
ACAATTAGTT TAAATGCTGC GAGTACACTG TTGGTTTCAG GCACCAAAGA AGGCACGGTG 
AATATTTGGG ACCTCACCAC GGCCACCATG TTGCACCAGA TCCCGTGTCA CTCGGGGACC 
GTGTGTGATG CTGCGTTTAG CCCAGACAGT CGCCACGTCC TCAGCACAGG GGAAGACGGC 
TGTCTAAACG TCATAGATGT GCAGACAGGA ATGCTCATCT CCTCTATGAC TTCAGACGAG 
CCCCAGAGAT GCTTTATCTG GGATGGAAAT TCTGTTTTAT CTGGAAGTCA GTCTGGTGAA 
CTGCTTGTTT GGGACCTCCT CGGAGGAAAA ATCAGTGAGA GAATCCAGGG CCACACAGGT 
GCTGTGACAT GTATATGGAT GAACGAACAG TGTAGCAGCA TCATCACAGG AGGGGAAGAC 
AGACAAATTA TGTTCTGGAA ATTGCAGTAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013964664.1
CDS	1..2793
Translation	MRQDMGAEGVCSLEGAVGKPGGQSAGAKDEKTLFSLLLLNLEEYYFEQHTANHIQNKGGQNERKIRGSLKICSKSVIFEPDAISQPIIKIPLRDCIKIGKHGENGSNRHFAKTKSGEISLIFSQVYFIKEHNIVAPYKIERGKMEYVFELDVSGKVEDVVETLLQLHRASCLDKLGDQTAMITAILQSRLARTSFDKNRFQSVSEKLHMECKAEMVTPLVTNPGHVCITDTNLYFQPLNGYPKPVVQITLQDVRRIYKRRHGLMPLGLEVFCTEDDLCSDIYLKFYEPQDRDDLYFYIATYLEHHVAERTAESYTLQWQRGHLSNYQYLLHLNNLADRSCNDLSQYPVFPWIISDYCSAELDLSNPGTFRDLSRPVGALNKERLERLLARYQEMPEPKFMYGSHYSSPGYVLFYLVRIAPEYMLCLQNGRFDNADRMFNSIGETWKNCLDGATDFKELIPEFYGDDVSFLVNNLKLDLGKRQGGQMVDDVELPPWARSPEDFLQKSKDALESNYVSEHLHEWIDLIFGYKQKGSDAVGAHNVFHPLTYEGGVDLNSIEDPDEKVAMLTQILEFGQTPKQLFVTPHPRRITPKFKNFSQTYSHNAPMADSPGEESFEDLTEESKTLAWNNIAKLQLHDQYKIHKEAVTGIAVSCNGSSVFTTSQDSTLKMFSKESKMLQRSISFSNMALSSCLLLPGDATVISSSWDNNIYFYSIAFARRQDTLMGHDDAVSKICWHDNRLYSASWDSTVKVWSGVPAEMTCTRRQRFDMLAELEHDVSVDTISLNAASTLLVSGTKEGTVNIWDLTTATMLHQIPCHSGTVCDAAFSPDSRHVLSTGEDGCLNVIDVQTGMLISSMTSDEPQRCFIWDGNSVLSGSQSGELLVWDLLGGKISERIQGHTGAVTCIWMNEQCSSIITGGEDRQIMFWKLQY

Target ORF information:

RefSeq Version	XM_014109189.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris neutral sphingomyelinase (N-SMase) activation associated factor (NSMAF), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014109189.1

ORF Insert Sequence:

ATGAGGCAGG ACATGGGAGC AGAGGGGGTG TGCAGCCTGG AAGGGGCGGT GGGGAAGCCT 
GGTGGTCAGT CAGCAGGAGC AAAGGATGAA AAAACTCTAT TTTCCTTGCT CCTGCTTAAC 
TTGGAGGAAT ACTACTTTGA ACAACATACC GCTAATCATA TTCAGAACAA GGGTGGTCAG 
AATGAAAGGA AAATCAGAGG CTCCTTAAAA ATATGTTCAA AATCAGTTAT TTTTGAACCA 
GATGCCATAT CTCAGCCCAT CATTAAGATT CCTTTGAGAG ATTGTATAAA AATAGGAAAA 
CATGGAGAAA ATGGATCCAA CAGACACTTT GCAAAGACAA AATCCGGGGA GATCTCACTC 
ATTTTCAGTC AGGTGTATTT CATTAAAGAA CACAACATTG TTGCACCATA TAAAATAGAA 
AGGGGCAAAA TGGAGTATGT CTTTGAATTG GACGTTTCAG GGAAAGTGGA AGATGTGGTG 
GAGACGTTGC TTCAGCTTCA CAGAGCATCC TGCCTCGACA AACTAGGAGA CCAGACTGCT 
ATGATAACAG CTATTTTGCA GTCACGTTTG GCTAGGACAT CATTTGACAA AAACAGATTC 
CAAAGTGTTT CTGAAAAGCT GCACATGGAA TGCAAAGCAG AAATGGTGAC ACCGCTGGTG 
ACGAATCCAG GACATGTGTG CATCACTGAC ACAAACCTGT ATTTTCAGCC TCTCAATGGG 
TACCCGAAAC CTGTGGTCCA GATAACACTC CAAGATGTCC GCCGCATTTA CAAAAGGAGG 
CACGGCCTCA TGCCTTTGGG CTTGGAAGTC TTTTGCACAG AAGATGATCT CTGTTCTGAC 
ATCTACTTAA AGTTCTATGA ACCTCAAGAT AGAGATGACC TCTATTTTTA TATTGCCACA 
TATCTCGAGC ACCACGTGGC CGAGCGCACG GCCGAGAGCT ACACGCTGCA GTGGCAGCGC 
GGCCACCTCT CCAACTACCA GTACCTGCTC CACCTCAACA ACCTGGCGGA CCGCAGCTGC 
AACGACCTGT CGCAGTACCC CGTGTTCCCC TGGATCATCA GCGACTACTG CAGTGCCGAG 
CTTGATTTGT CAAATCCAGG AACCTTCCGG GATCTTAGCA GGCCAGTAGG GGCCCTAAAC 
AAGGAAAGGC TGGAGAGACT CCTGGCCCGC TACCAGGAAA TGCCAGAGCC AAAGTTCATG 
TACGGCAGTC ACTACTCATC CCCGGGTTAT GTGCTATTTT ATCTTGTTAG GATCGCGCCC 
GAGTACATGC TGTGCCTGCA GAATGGAAGA TTTGATAATG CAGACCGAAT GTTCAACAGT 
ATTGGAGAAA CTTGGAAAAA CTGTTTGGAT GGTGCAACAG ATTTTAAAGA GTTGATTCCA 
GAATTCTATG GTGATGATGT CAGCTTTTTA GTCAATAATC TGAAGTTGGA TCTGGGAAAG 
AGACAAGGAG GACAGATGGT TGATGATGTG GAACTTCCCC CCTGGGCACG GAGTCCTGAG 
GATTTTCTCC AGAAGAGCAA AGACGCGCTG GAAAGCAATT ATGTGTCCGA GCACCTCCAC 
GAGTGGATCG ATCTGATATT TGGCTACAAG CAAAAAGGGA GCGATGCCGT TGGGGCCCAT 
AATGTATTTC ATCCCCTGAC CTATGAAGGA GGCGTAGACC TTAACAGCAT TGAGGACCCT 
GATGAGAAAG TAGCCATGCT GACCCAAATC TTGGAGTTTG GCCAGACGCC GAAACAACTC 
TTCGTGACGC CACATCCACG ACGGATCACC CCAAAGTTTA AAAATTTCTC CCAAACCTAT 
AGTCATAATG CTCCCATGGC AGATTCTCCA GGTGAGGAGT CCTTTGAAGA CCTGACGGAA 
GAAAGCAAAA CCCTGGCCTG GAATAACATC GCCAAGCTGC AGTTACACGA CCAGTATAAG 
ATCCACAAAG AGGCAGTTAC TGGGATAGCG GTCTCTTGCA ACGGGTCCTC TGTCTTTACA 
ACCTCACAAG ATTCCACCTT GAAGATGTTC TCTAAGGAAT CCAAAATGCT ACAAAGAAGC 
ATATCATTTT CAAATATGGC TTTATCATCT TGTTTACTTT TGCCAGGAGA TGCCACTGTC 
ATAAGTTCTT CATGGGATAA TAACATCTAT TTTTATTCTA TAGCATTTGC AAGACGCCAG 
GACACGTTAA TGGGACACGA TGATGCCGTT AGTAAGATCT GTTGGCATGA CAACCGACTG 
TATTCTGCAT CGTGGGACTC CACAGTGAAG GTGTGGTCTG GTGTTCCTGC AGAAATGACC 
TGCACCAGGA GACAGCGGTT TGACATGCTG GCCGAGCTGG AACACGACGT CAGTGTAGAT 
ACAATTAGTT TAAATGCTGC GAGTACACTG TTGGTTTCAG GCACCAAAGA AGGCACGGTG 
AATATTTGGG ACCTCACCAC GGCCACCATG TTGCACCAGA TCCCGTGTCA CTCGGGGACC 
GTGTGTGATG CTGCGTTTAG CCCAGACAGT CGCCACGTCC TCAGCACAGG GGAAGACGGC 
TGTCTAAACG TCATAGATGT GCAGACAGGA ATGCTCATCT CCTCTATGAC TTCAGACGAG 
CCCCAGAGAT GCTTTATCTG GGATGGAAAT TCTGTTTTAT CTGGAAGTCA GTCTGGTGAA 
CTGCTTGTTT GGGACCTCCT CGGAGGAAAA ATCAGTGAGA GAATCCAGGG CCACACAGGT 
GCTGTGACAT GTATATGGAT GAACGAACAG TGTAGCAGCA TCATCACAGG AGGGGAAGAC 
AGACAAATTA TGTTCTGGAA ATTGCAGTAT TAA