gRNAs
The following Arhgap35 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Arhgap35 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | ORa10371 | |
| Clone ID Related Accession (Same CDS sequence) | XM_008758919.1 , NM_001271132.1 , XM_006228412.3 | |
| Accession Version | NM_001271132.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 4500bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-12-24 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | rho GTPase-activating protein 35 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07002648.1 and AABR07002647.1. On Sep 1, 2012 this sequence version replaced XM_001053554.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01906353, SAMN01906354 [ECO:0000348] ##Evidence-Data-END## | |
1 | ATGATGATGG CAAGAAAGCA AGATGTCCGA ATCCCCACCT ACAACATCAG TGTGGTGGGA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001258061.1 |
| CDS | 691..5190 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_001271132.1 |
1 | ATGATGATGG CAAGAAAGCA AGATGTCCGA ATCCCCACCT ACAACATCAG TGTGGTGGGA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ORa10371 | |
| Clone ID Related Accession (Same CDS sequence) | XM_008758919.1 , NM_001271132.1 , XM_006228412.3 | |
| Accession Version | XM_008758919.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 4500bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-07-25 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | rho GTPase-activating protein 35 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGATGATGG CAAGAAAGCA AGATGTCCGA ATCCCCACCT ACAACATCAG TGTGGTGGGA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_008757141.1 |
| CDS | 309..4808 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_008758919.1 |
1 | ATGATGATGG CAAGAAAGCA AGATGTCCGA ATCCCCACCT ACAACATCAG TGTGGTGGGA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ORa10371 | |
| Clone ID Related Accession (Same CDS sequence) | XM_008758919.1 , NM_001271132.1 , XM_006228412.3 | |
| Accession Version | XM_006228412.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 4500bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-07-25 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | rho GTPase-activating protein 35 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006228412.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGATGATGG CAAGAAAGCA AGATGTCCGA ATCCCCACCT ACAACATCAG TGTGGTGGGA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_006228474.1 |
| CDS | 1255..5754 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_006228412.3 |
1 | ATGATGATGG CAAGAAAGCA AGATGTCCGA ATCCCCACCT ACAACATCAG TGTGGTGGGA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Symmetry breaking in spreading RAT2 fibroblasts requires the MAPK/ERK pathway scaffold RACK1 that integrates FAK, p190A-RhoGAP and ERK2 signaling. |
Cancer-associated mutations in the protrusion-targeting region of p190RhoGAP impact tumor cell migration. |
A Point Mutation in p190A RhoGAP Affects Ciliogenesis and Leads to Glomerulocystic Kidney Defects. |
Integrin ?3 is required for late postnatal stability of dendrite arbors, dendritic spines and synapses, and mouse behavior. |
