arhgap35 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	arhgap35
Entrez Gene ID	564565
Full Name	Rho GTPase activating protein 35b
Synonyms	arhgap35,si:ch211-139a5.8
General protein information	Preferred Names Rho GTPase activating protein 35b Names Rho GTPase activating protein 35b rho GTPase-activating protein 35
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003198643.5	XP_003198691.2	rho GTPase-activating protein 35
XM_009301690.3	XP_009299965.1	rho GTPase-activating protein 35
XM_021476553.1	XP_021332228.1	rho GTPase-activating protein 35
XM_009301690.2	XP_009299965.1	rho GTPase-activating protein 35
XM_003198643.4	XP_003198691.2	rho GTPase-activating protein 35
XM_021476554.1	XP_021332229.1	rho GTPase-activating protein 35

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ARHGAP35	XP_003316510.1
	GRLF1	XP_001112719.1
Rattus norvegicus (Norway rat)	Arhgap35	NP_001258061.1
Gallus gallus (chicken)	ARHGAP35	XP_003643808.2
Homo sapiens (human)	ARHGAP35	NP_004482.4
Bos taurus (cattle)	ARHGAP35	NP_001179327.1
Danio rerio (zebrafish)	arhgap35	XP_003198691.2
Danio rerio (zebrafish)	LOC100004299	XP_001343636.1
Xenopus tropicalis (tropical clawed frog)	arhgap35	XP_004917009.1
Drosophila melanogaster (fruit fly)	RhoGAPp190	NP_001259658.1
Mus musculus (house mouse)	Grlf1	NP_766327.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000870	XP_316844.5
Canis lupus familiaris (dog)	ARHGAP35	NP_001003022.1

Gene Ontology

The following arhgap35 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the arhgap35 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa44355	XM_009301690.3 Latest version!	Danio rerio Rho GTPase activating protein 35b (arhgap35), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa44355	XM_003198643.5 Latest version!	Danio rerio Rho GTPase activating protein 35b (arhgap35), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa44355	XM_021476554.1 Latest version!	Danio rerio Rho GTPase activating protein 35b (arhgap35), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa44355	XM_021476553.1 Latest version!	Danio rerio Rho GTPase activating protein 35b (arhgap35), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
View Old Accession Versions >>
ODa44355	XM_009301690.2	Danio rerio Rho GTPase activating protein 35b (arhgap35), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa44355	XM_003198643.4	Danio rerio Rho GTPase activating protein 35b (arhgap35), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

arhgap35 ( XM_003198643.5 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_003198691.2 Danio rerio Rho GTPase activating protein 35b (arhgap35), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa44355
Clone ID Related Accession (Same CDS sequence)	XM_003198643.5 , XM_009301690.3 , XM_021476553.1 , XM_009301690.2 , XM_003198643.4 , XM_021476554.1
Accession Version	XM_003198643.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4554bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	rho GTPase-activating protein 35
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394596.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_003198643.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGG CTAAAAAGCA AGAATCCCGG CTGCCAATCT ACAACCTTGT TGTTGTTGGC 
CTGTCGGGAA CTGAGAAGGA GAAAGGGCAA TGTGGAGTTG GCAAATCTTG CCTTTGTAAT 
CGCTTTGTTC GCCCTAGTGC AGATGACTTC CATCTCGATC ACACCTCTGT GCTAAGTACT 
AGTGACTTTG GTGGCCGGGT TGTCAACAAT GACCATTTCC TGTTCTGGGG GGAGGTTGGG 
AGAGTTTTGG AGGATGGTGC AGACTGCAGA ATGCATGTGG TCGAGCAGAC AGAATTTATT 
GATGACCAGA CTTTCCAGCC CCATCGTAGC ACAGCCTTAC TGCCCTACAT CAAAAGAGCT 
GCCTCCACCA AATTGGCTTC TGCGGAAAAA CTCATGTACT TCTGCACTGA TCAGTTGGGC 
CTGGAGCAGG ACTTCGAACA GAAACAAATG CCTGAAGGCA AGTTGCAGGT CGATGGCTTT 
CTGCTTTGTG TTGATGTCAG TCGTGGTATG AACCGCAACT TTGATGATCA AATGAAATTT 
GTCACCAACT TGTACAATCA CTTAAGCAAG ACAAAAAAGC CTATTGTGCT GGTTCTCACC 
AAGTGTGATG AGGGTGTGGA GCGATACATC AAGGATTCTC ATACCTTTGC CATTACAAAA 
AAGAACCTGC AAGTTGTTGA GACTTCGGCT CGATCCAATA TCAATGTTGA CTTGGCTTTT 
CTTACCTTAA TTCAACTCAT TGATAAGGGA AAAGGTAAGC CTAAGATCAT ACCTTACTTT 
GAGGCATTGA AACACCAGAG TCAACTGATT GCCTCCGCAA AAGACCGCTA TGAATGGTTG 
GTGAACCACA TTGTGAAGAA TCACAATGAA ACTTGGCCAA ACGTCAGCAG ACGTATGCAG 
ACTTCACCCG AATATAAGGA ATATGTGTTT TTAGAAGGCA CAGGTAAAGC TAAGAAACTA 
TTTCAACAGC ACATTCACCG ACTTAAACAA GAGCATACTG AAAGACGTCG TAAAGCATAC 
TTGAGTGCTC TTCCACTCGC CTTAAGTAGC TTGGTGTCGG AGTTGGATGA AATTGAGCAT 
TTAAGCTGGT CTGGGGTGCA AAAAGTGCTT GAGTCTAAAA AAGACTTTGA TCATTGGTTT 
GTGGTACTGG ACGATGCACC ATGGGAAGAC ACACCACATC TTGACAATAT GGAGGATGAC 
CGCATCCCCT TTGATGTGCT AGACACCACG GCTGCTGAAA TCCTCTTTGA TGCACACCTT 
GAACACTTGC GCAATGAATG CAAGCGTGCC CAGATGCGTC ATGAGTTCAA AGGAAAATTA 
GCATCTTCAC CTTTCGTTAC TCCAGGCAAG CCCTGGGAGG AGGCTCGAAG CTTCATCATG 
AATGAAGACT TTTACCAGTG GCTTGAGGAG CCAGAATATT TGGACATTTA TAACCGTCAC 
CAGAAAGCAA TCATTGACAG GGCAAAAGAG GATTTTCAGG AGCTTCTGCT GGAGTACTCT 
GAACTCTTCT ACGAACTTGA GGTGGATGCC AAACCTAGTA AAGAGAAAAT GGGAGCAATT 
CAAGAAGTGT TAGGTGAAGA ACAACGCTTC AAGGCACTTC AGAAACTGCA AGCTGAAAGG 
GATGCTTTAG TACTGAAACA CATCCATTTT GTTTATCACC CTACGAAGGA AACATGCCCA 
AACTGCCCAC ACTGTGTTGA CAGCAAAATT GAGCAAATTC TAGCTTCATG CTTCCCTGCA 
CAATATCCTT CCTTTGGAAA GTCGCATATA ACTGATGCAA AGGTAGATAG GATCAATCTT 
GTTATACTGG GTAAAGATGG ACTTGCTAGA GAATTAGCCA ATGAAATTCG GGCACTGTGT 
ACTAATGATG ATCATTATGT GCTTGAAGGA AGAATGTATG AGCTTGTCCT TCGGCCAATT 
GAGGGCAATG TTAGACTTCC TGTTAATTCG TTTCATACAT CCACATTTAC TCCCCATGGA 
TGCCTGTGTT TGTACAATTC AAAAGAGTCC TTGTCCTATG TTGTAGAAAG CATTGAGAAG 
TTGCGAGAGT CCACACTTGG CAGAAGGGAC AACCATATGG CTCAGCTTCC GTTGTCCCTC 
TTATTGGTAA GCAAAAGAGG TGTTGGAGGA CTGAGTGATA TTGGTGGTGA GACTGCTCAA 
GCGTTAATCT CACAGGGTCA GCAAGTAGCC ATAAAACTGC AGTGTAACTA CCTGGAACCT 
TCCTCCCCAG GCACTGGTTA TGGGCGAAAC GTGAATGAAA AGCAGATCAA CCAGGTTTTG 
AAAGCTCTAC TTGAAACAAG GAGGACTTCT ACATTTCGTA GCTGTTCTCC ACCTGCACCT 
CCACTGCCTC CATGTATTCG AGACTTTCAG CAAGACCAGA GCCCAGAGGC TGATTTGCGT 
ATCGTCATGT GCCTAATGTG TGGAGACTCT TATGACATCG ATCAACTTCT TGCACCATTC 
CTGCTGCCTC AGCATTGCAA GCCAACTTCT CCCCAGACAA GTGGCACTTC AGTGCTGCTA 
GAACAAACAA TTGGTGGCCA CAAGCTTACG ATTGAGCTTT CTCTGCTGTC TTACCACTCT 
TCTTTTGCTA CTCGGAAGAG TCGACTGGTG CATGGCTACA TAGCTGTATA TTCTGCTAGC 
CGCAAGGCTT CTTTTGAAAC CCTGTGTGCC TTTTTGTGTG AGGTTCAGGA CATCATCCCG 
GTACAGATGA TTGCAGTGGG GGAAACACAG ATTGAGCTTC AGGACTCTGA GTCTGCTCGT 
GAATTGTTAG TCCAAGGTGA AGAGCTGGCA CATGAACTTG AAGGACGATT TGGCAGTATT 
GTCTGTGGTC ATGGCGGCGT TGTTGGAGGA CTACACAAAC TAGACCTTTT AGAACAATTT 
TTCACAGAAG TATTAGAAAA GAAAACCATA GTTGAAGCCA GCCATATGTA TGAAAGCATG 
GCAGAAGCCA GTAGCACAAA TGAGAATGTG TATTCGCCAC ACTGTGGCTC CCCAAGCCCT 
GTCACAATGC TGTTAGATTC TGAGGAAGAC ATGGAAGCTT CCCCGCCTTA CCATGATGGC 
ACACTTACCT CTCACGGTGG ATTCAAACTG CCTGACCTGG ACTCAGGTGA CACATTCTCT 
GTGATCTCTG ACCTTAGCAC CTTTGAGAAC AAGCTAAATA ATAAAGTTCC TCCACAAGTG 
AGACCCAAAC CTAGTGTCAC ATTTGACCTT AGAAAGCCAA GTTTTAACCC CTATGTGGAT 
GGAGTCAGCC ACCGCCGTTC CCTCACATCC AATGTGACGT GGCCTCTAGG AGGAGATTAT 
GATCCTTCAG ACTATGCTGA GCCAATGGAT GCTGTCTCTA AGCCACGCCC CAGCTATGAA 
GACAGCATAT ATTCTGTGCC TCATGACAGC ACACAAGGTA AAATTATTAC TATACGCAAT 
TCAAATCGAA TCCACTCCAA CGGAGGAGGA AATGGTTCAG ACAGTGAAGG GGATGGCAGC 
TCATTGGAGA GACGTCGCAA GTTTTCAGCA GCAAGGGTTA AGCCACGGCT TTACCGTGAT 
CGTTCCAAAC GTCTGGGGAA GTTCAGCAGT TTCCGCACTA GTTTCTCTAT TGGTAGCGAT 
GATGAGATTG GCACACTCTC AAGGTCAAAA GAGGATGATG TTTCACCACT GAAGACAGAT 
ATAACAAATG AAGAAGGAGA AGATCCCAAA AAGAGAAACA TCCTGAAAAG TCTCCGCAGA 
CCAGGCAAAA AAGCAAGACC AAAGCCTCGT CATTCCATCT CTAAGCCTTT GGAGAGTAAC 
TACTTTGGAG TGCCTCTGGT CAATGTTGTT TCCTTCGAAC GACCTATTCC AGTTTTCATT 
GACAAGTGTA TTCGTTATAT TGAAGCCACA GGGTTAACAA CAGAGGGTAT CTACAGGGTA 
AGTGGGAATA AGGCGGAGAT CGAGGGCATG CAGAGGCAGT TTGAACAGGA CCACAATCTG 
GACTTTGTGG AGAAGGACTT CACTGTGAAC ACAGTTGCCG GGGCAATGAA AAGTTTCTTC 
TCTGAGCTGC CTGATCCTCT GGTTCCTTAC AGTTCACAGG AGGAGCTGGT GGAAGCTTTC 
AAAATTAATG ATCGAGAACA GAGACTTCAC ACAATGAAGG ACGTGTTAAG ACGATTTCCC 
AGGGAGAATT TTGATGTCTT CAAGTATGTA ATGAGCCATC TAAACAAGGT TGGTCAGTGG 
AACAGAGTCA ACTTAATGAC CAGTGAGAAT CTCTCCATCT GTTTCTGGCC AACACTGATG 
CGGCCAGACT TCACTTCTAT GGATGCCCTG ACCGCCACAC GCACCTACCA GACAATCATC 
GAGACGTTTA TCCATCAATG TGCTTTCTTC TTTTACAACC AGCCTCCTGC TGAATCGCCC 
ACTGGCCTAT TCGGCCAGCC TGGACCAATG GTGCCCTGTG GATTGCCCCC ATCCCCCACA 
GCTGCTCCTT CTTATTGCCC TTCGACCCCA CATTTTACCC CTCTTCTTCA GTCCCCGCCT 
CATTCTCCCC CCCAGAGCCC ACACTCCGCC CCTGCAGAAC CACAAACACT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003198691.2
CDS	1264..5817
Translation	MMMAKKQESRLPIYNLVVVGLSGTEKEKGQCGVGKSCLCNRFVRPSADDFHLDHTSVLSTSDFGGRVVNNDHFLFWGEVGRVLEDGADCRMHVVEQTEFIDDQTFQPHRSTALLPYIKRAASTKLASAEKLMYFCTDQLGLEQDFEQKQMPEGKLQVDGFLLCVDVSRGMNRNFDDQMKFVTNLYNHLSKTKKPIVLVLTKCDEGVERYIKDSHTFAITKKNLQVVETSARSNINVDLAFLTLIQLIDKGKGKPKIIPYFEALKHQSQLIASAKDRYEWLVNHIVKNHNETWPNVSRRMQTSPEYKEYVFLEGTGKAKKLFQQHIHRLKQEHTERRRKAYLSALPLALSSLVSELDEIEHLSWSGVQKVLESKKDFDHWFVVLDDAPWEDTPHLDNMEDDRIPFDVLDTTAAEILFDAHLEHLRNECKRAQMRHEFKGKLASSPFVTPGKPWEEARSFIMNEDFYQWLEEPEYLDIYNRHQKAIIDRAKEDFQELLLEYSELFYELEVDAKPSKEKMGAIQEVLGEEQRFKALQKLQAERDALVLKHIHFVYHPTKETCPNCPHCVDSKIEQILASCFPAQYPSFGKSHITDAKVDRINLVILGKDGLARELANEIRALCTNDDHYVLEGRMYELVLRPIEGNVRLPVNSFHTSTFTPHGCLCLYNSKESLSYVVESIEKLRESTLGRRDNHMAQLPLSLLLVSKRGVGGLSDIGGETAQALISQGQQVAIKLQCNYLEPSSPGTGYGRNVNEKQINQVLKALLETRRTSTFRSCSPPAPPLPPCIRDFQQDQSPEADLRIVMCLMCGDSYDIDQLLAPFLLPQHCKPTSPQTSGTSVLLEQTIGGHKLTIELSLLSYHSSFATRKSRLVHGYIAVYSASRKASFETLCAFLCEVQDIIPVQMIAVGETQIELQDSESARELLVQGEELAHELEGRFGSIVCGHGGVVGGLHKLDLLEQFFTEVLEKKTIVEASHMYESMAEASSTNENVYSPHCGSPSPVTMLLDSEEDMEASPPYHDGTLTSHGGFKLPDLDSGDTFSVISDLSTFENKLNNKVPPQVRPKPSVTFDLRKPSFNPYVDGVSHRRSLTSNVTWPLGGDYDPSDYAEPMDAVSKPRPSYEDSIYSVPHDSTQGKIITIRNSNRIHSNGGGNGSDSEGDGSSLERRRKFSAARVKPRLYRDRSKRLGKFSSFRTSFSIGSDDEIGTLSRSKEDDVSPLKTDITNEEGEDPKKRNILKSLRRPGKKARPKPRHSISKPLESNYFGVPLVNVVSFERPIPVFIDKCIRYIEATGLTTEGIYRVSGNKAEIEGMQRQFEQDHNLDFVEKDFTVNTVAGAMKSFFSELPDPLVPYSSQEELVEAFKINDREQRLHTMKDVLRRFPRENFDVFKYVMSHLNKVGQWNRVNLMTSENLSICFWPTLMRPDFTSMDALTATRTYQTIIETFIHQCAFFFYNQPPAESPTGLFGQPGPMVPCGLPPSPTAAPSYCPSTPHFTPLLQSPPHSPPQSPHSAPAEPQTL

Target ORF information:

RefSeq Version	XM_003198643.5
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Rho GTPase activating protein 35b (arhgap35), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003198643.5

ORF Insert Sequence:

ATGATGATGG CTAAAAAGCA AGAATCCCGG CTGCCAATCT ACAACCTTGT TGTTGTTGGC 
CTGTCGGGAA CTGAGAAGGA GAAAGGGCAA TGTGGAGTTG GCAAATCTTG CCTTTGTAAT 
CGCTTTGTTC GCCCTAGTGC AGATGACTTC CATCTCGATC ACACCTCTGT GCTAAGTACT 
AGTGACTTTG GTGGCCGGGT TGTCAACAAT GACCATTTCC TGTTCTGGGG GGAGGTTGGG 
AGAGTTTTGG AGGATGGTGC AGACTGCAGA ATGCATGTGG TCGAGCAGAC AGAATTTATT 
GATGACCAGA CTTTCCAGCC CCATCGTAGC ACAGCCTTAC TGCCCTACAT CAAAAGAGCT 
GCCTCCACCA AATTGGCTTC TGCGGAAAAA CTCATGTACT TCTGCACTGA TCAGTTGGGC 
CTGGAGCAGG ACTTCGAACA GAAACAAATG CCTGAAGGCA AGTTGCAGGT CGATGGCTTT 
CTGCTTTGTG TTGATGTCAG TCGTGGTATG AACCGCAACT TTGATGATCA AATGAAATTT 
GTCACCAACT TGTACAATCA CTTAAGCAAG ACAAAAAAGC CTATTGTGCT GGTTCTCACC 
AAGTGTGATG AGGGTGTGGA GCGATACATC AAGGATTCTC ATACCTTTGC CATTACAAAA 
AAGAACCTGC AAGTTGTTGA GACTTCGGCT CGATCCAATA TCAATGTTGA CTTGGCTTTT 
CTTACCTTAA TTCAACTCAT TGATAAGGGA AAAGGTAAGC CTAAGATCAT ACCTTACTTT 
GAGGCATTGA AACACCAGAG TCAACTGATT GCCTCCGCAA AAGACCGCTA TGAATGGTTG 
GTGAACCACA TTGTGAAGAA TCACAATGAA ACTTGGCCAA ACGTCAGCAG ACGTATGCAG 
ACTTCACCCG AATATAAGGA ATATGTGTTT TTAGAAGGCA CAGGTAAAGC TAAGAAACTA 
TTTCAACAGC ACATTCACCG ACTTAAACAA GAGCATACTG AAAGACGTCG TAAAGCATAC 
TTGAGTGCTC TTCCACTCGC CTTAAGTAGC TTGGTGTCGG AGTTGGATGA AATTGAGCAT 
TTAAGCTGGT CTGGGGTGCA AAAAGTGCTT GAGTCTAAAA AAGACTTTGA TCATTGGTTT 
GTGGTACTGG ACGATGCACC ATGGGAAGAC ACACCACATC TTGACAATAT GGAGGATGAC 
CGCATCCCCT TTGATGTGCT AGACACCACG GCTGCTGAAA TCCTCTTTGA TGCACACCTT 
GAACACTTGC GCAATGAATG CAAGCGTGCC CAGATGCGTC ATGAGTTCAA AGGAAAATTA 
GCATCTTCAC CTTTCGTTAC TCCAGGCAAG CCCTGGGAGG AGGCTCGAAG CTTCATCATG 
AATGAAGACT TTTACCAGTG GCTTGAGGAG CCAGAATATT TGGACATTTA TAACCGTCAC 
CAGAAAGCAA TCATTGACAG GGCAAAAGAG GATTTTCAGG AGCTTCTGCT GGAGTACTCT 
GAACTCTTCT ACGAACTTGA GGTGGATGCC AAACCTAGTA AAGAGAAAAT GGGAGCAATT 
CAAGAAGTGT TAGGTGAAGA ACAACGCTTC AAGGCACTTC AGAAACTGCA AGCTGAAAGG 
GATGCTTTAG TACTGAAACA CATCCATTTT GTTTATCACC CTACGAAGGA AACATGCCCA 
AACTGCCCAC ACTGTGTTGA CAGCAAAATT GAGCAAATTC TAGCTTCATG CTTCCCTGCA 
CAATATCCTT CCTTTGGAAA GTCGCATATA ACTGATGCAA AGGTAGATAG GATCAATCTT 
GTTATACTGG GTAAAGATGG ACTTGCTAGA GAATTAGCCA ATGAAATTCG GGCACTGTGT 
ACTAATGATG ATCATTATGT GCTTGAAGGA AGAATGTATG AGCTTGTCCT TCGGCCAATT 
GAGGGCAATG TTAGACTTCC TGTTAATTCG TTTCATACAT CCACATTTAC TCCCCATGGA 
TGCCTGTGTT TGTACAATTC AAAAGAGTCC TTGTCCTATG TTGTAGAAAG CATTGAGAAG 
TTGCGAGAGT CCACACTTGG CAGAAGGGAC AACCATATGG CTCAGCTTCC GTTGTCCCTC 
TTATTGGTAA GCAAAAGAGG TGTTGGAGGA CTGAGTGATA TTGGTGGTGA GACTGCTCAA 
GCGTTAATCT CACAGGGTCA GCAAGTAGCC ATAAAACTGC AGTGTAACTA CCTGGAACCT 
TCCTCCCCAG GCACTGGTTA TGGGCGAAAC GTGAATGAAA AGCAGATCAA CCAGGTTTTG 
AAAGCTCTAC TTGAAACAAG GAGGACTTCT ACATTTCGTA GCTGTTCTCC ACCTGCACCT 
CCACTGCCTC CATGTATTCG AGACTTTCAG CAAGACCAGA GCCCAGAGGC TGATTTGCGT 
ATCGTCATGT GCCTAATGTG TGGAGACTCT TATGACATCG ATCAACTTCT TGCACCATTC 
CTGCTGCCTC AGCATTGCAA GCCAACTTCT CCCCAGACAA GTGGCACTTC AGTGCTGCTA 
GAACAAACAA TTGGTGGCCA CAAGCTTACG ATTGAGCTTT CTCTGCTGTC TTACCACTCT 
TCTTTTGCTA CTCGGAAGAG TCGACTGGTG CATGGCTACA TAGCTGTATA TTCTGCTAGC 
CGCAAGGCTT CTTTTGAAAC CCTGTGTGCC TTTTTGTGTG AGGTTCAGGA CATCATCCCG 
GTACAGATGA TTGCAGTGGG GGAAACACAG ATTGAGCTTC AGGACTCTGA GTCTGCTCGT 
GAATTGTTAG TCCAAGGTGA AGAGCTGGCA CATGAACTTG AAGGACGATT TGGCAGTATT 
GTCTGTGGTC ATGGCGGCGT TGTTGGAGGA CTACACAAAC TAGACCTTTT AGAACAATTT 
TTCACAGAAG TATTAGAAAA GAAAACCATA GTTGAAGCCA GCCATATGTA TGAAAGCATG 
GCAGAAGCCA GTAGCACAAA TGAGAATGTG TATTCGCCAC ACTGTGGCTC CCCAAGCCCT 
GTCACAATGC TGTTAGATTC TGAGGAAGAC ATGGAAGCTT CCCCGCCTTA CCATGATGGC 
ACACTTACCT CTCACGGTGG ATTCAAACTG CCTGACCTGG ACTCAGGTGA CACATTCTCT 
GTGATCTCTG ACCTTAGCAC CTTTGAGAAC AAGCTAAATA ATAAAGTTCC TCCACAAGTG 
AGACCCAAAC CTAGTGTCAC ATTTGACCTT AGAAAGCCAA GTTTTAACCC CTATGTGGAT 
GGAGTCAGCC ACCGCCGTTC CCTCACATCC AATGTGACGT GGCCTCTAGG AGGAGATTAT 
GATCCTTCAG ACTATGCTGA GCCAATGGAT GCTGTCTCTA AGCCACGCCC CAGCTATGAA 
GACAGCATAT ATTCTGTGCC TCATGACAGC ACACAAGGTA AAATTATTAC TATACGCAAT 
TCAAATCGAA TCCACTCCAA CGGAGGAGGA AATGGTTCAG ACAGTGAAGG GGATGGCAGC 
TCATTGGAGA GACGTCGCAA GTTTTCAGCA GCAAGGGTTA AGCCACGGCT TTACCGTGAT 
CGTTCCAAAC GTCTGGGGAA GTTCAGCAGT TTCCGCACTA GTTTCTCTAT TGGTAGCGAT 
GATGAGATTG GCACACTCTC AAGGTCAAAA GAGGATGATG TTTCACCACT GAAGACAGAT 
ATAACAAATG AAGAAGGAGA AGATCCCAAA AAGAGAAACA TCCTGAAAAG TCTCCGCAGA 
CCAGGCAAAA AAGCAAGACC AAAGCCTCGT CATTCCATCT CTAAGCCTTT GGAGAGTAAC 
TACTTTGGAG TGCCTCTGGT CAATGTTGTT TCCTTCGAAC GACCTATTCC AGTTTTCATT 
GACAAGTGTA TTCGTTATAT TGAAGCCACA GGGTTAACAA CAGAGGGTAT CTACAGGGTA 
AGTGGGAATA AGGCGGAGAT CGAGGGCATG CAGAGGCAGT TTGAACAGGA CCACAATCTG 
GACTTTGTGG AGAAGGACTT CACTGTGAAC ACAGTTGCCG GGGCAATGAA AAGTTTCTTC 
TCTGAGCTGC CTGATCCTCT GGTTCCTTAC AGTTCACAGG AGGAGCTGGT GGAAGCTTTC 
AAAATTAATG ATCGAGAACA GAGACTTCAC ACAATGAAGG ACGTGTTAAG ACGATTTCCC 
AGGGAGAATT TTGATGTCTT CAAGTATGTA ATGAGCCATC TAAACAAGGT TGGTCAGTGG 
AACAGAGTCA ACTTAATGAC CAGTGAGAAT CTCTCCATCT GTTTCTGGCC AACACTGATG 
CGGCCAGACT TCACTTCTAT GGATGCCCTG ACCGCCACAC GCACCTACCA GACAATCATC 
GAGACGTTTA TCCATCAATG TGCTTTCTTC TTTTACAACC AGCCTCCTGC TGAATCGCCC 
ACTGGCCTAT TCGGCCAGCC TGGACCAATG GTGCCCTGTG GATTGCCCCC ATCCCCCACA 
GCTGCTCCTT CTTATTGCCC TTCGACCCCA CATTTTACCC CTCTTCTTCA GTCCCCGCCT 
CATTCTCCCC CCCAGAGCCC ACACTCCGCC CCTGCAGAAC CACAAACACT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgap35 ( XM_009301690.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009299965.1 Danio rerio Rho GTPase activating protein 35b (arhgap35), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa44355
Clone ID Related Accession (Same CDS sequence)	XM_003198643.5 , XM_009301690.3 , XM_021476553.1 , XM_009301690.2 , XM_003198643.4 , XM_021476554.1
Accession Version	XM_009301690.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4554bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	rho GTPase-activating protein 35
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394596.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009301690.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGG CTAAAAAGCA AGAATCCCGG CTGCCAATCT ACAACCTTGT TGTTGTTGGC 
CTGTCGGGAA CTGAGAAGGA GAAAGGGCAA TGTGGAGTTG GCAAATCTTG CCTTTGTAAT 
CGCTTTGTTC GCCCTAGTGC AGATGACTTC CATCTCGATC ACACCTCTGT GCTAAGTACT 
AGTGACTTTG GTGGCCGGGT TGTCAACAAT GACCATTTCC TGTTCTGGGG GGAGGTTGGG 
AGAGTTTTGG AGGATGGTGC AGACTGCAGA ATGCATGTGG TCGAGCAGAC AGAATTTATT 
GATGACCAGA CTTTCCAGCC CCATCGTAGC ACAGCCTTAC TGCCCTACAT CAAAAGAGCT 
GCCTCCACCA AATTGGCTTC TGCGGAAAAA CTCATGTACT TCTGCACTGA TCAGTTGGGC 
CTGGAGCAGG ACTTCGAACA GAAACAAATG CCTGAAGGCA AGTTGCAGGT CGATGGCTTT 
CTGCTTTGTG TTGATGTCAG TCGTGGTATG AACCGCAACT TTGATGATCA AATGAAATTT 
GTCACCAACT TGTACAATCA CTTAAGCAAG ACAAAAAAGC CTATTGTGCT GGTTCTCACC 
AAGTGTGATG AGGGTGTGGA GCGATACATC AAGGATTCTC ATACCTTTGC CATTACAAAA 
AAGAACCTGC AAGTTGTTGA GACTTCGGCT CGATCCAATA TCAATGTTGA CTTGGCTTTT 
CTTACCTTAA TTCAACTCAT TGATAAGGGA AAAGGTAAGC CTAAGATCAT ACCTTACTTT 
GAGGCATTGA AACACCAGAG TCAACTGATT GCCTCCGCAA AAGACCGCTA TGAATGGTTG 
GTGAACCACA TTGTGAAGAA TCACAATGAA ACTTGGCCAA ACGTCAGCAG ACGTATGCAG 
ACTTCACCCG AATATAAGGA ATATGTGTTT TTAGAAGGCA CAGGTAAAGC TAAGAAACTA 
TTTCAACAGC ACATTCACCG ACTTAAACAA GAGCATACTG AAAGACGTCG TAAAGCATAC 
TTGAGTGCTC TTCCACTCGC CTTAAGTAGC TTGGTGTCGG AGTTGGATGA AATTGAGCAT 
TTAAGCTGGT CTGGGGTGCA AAAAGTGCTT GAGTCTAAAA AAGACTTTGA TCATTGGTTT 
GTGGTACTGG ACGATGCACC ATGGGAAGAC ACACCACATC TTGACAATAT GGAGGATGAC 
CGCATCCCCT TTGATGTGCT AGACACCACG GCTGCTGAAA TCCTCTTTGA TGCACACCTT 
GAACACTTGC GCAATGAATG CAAGCGTGCC CAGATGCGTC ATGAGTTCAA AGGAAAATTA 
GCATCTTCAC CTTTCGTTAC TCCAGGCAAG CCCTGGGAGG AGGCTCGAAG CTTCATCATG 
AATGAAGACT TTTACCAGTG GCTTGAGGAG CCAGAATATT TGGACATTTA TAACCGTCAC 
CAGAAAGCAA TCATTGACAG GGCAAAAGAG GATTTTCAGG AGCTTCTGCT GGAGTACTCT 
GAACTCTTCT ACGAACTTGA GGTGGATGCC AAACCTAGTA AAGAGAAAAT GGGAGCAATT 
CAAGAAGTGT TAGGTGAAGA ACAACGCTTC AAGGCACTTC AGAAACTGCA AGCTGAAAGG 
GATGCTTTAG TACTGAAACA CATCCATTTT GTTTATCACC CTACGAAGGA AACATGCCCA 
AACTGCCCAC ACTGTGTTGA CAGCAAAATT GAGCAAATTC TAGCTTCATG CTTCCCTGCA 
CAATATCCTT CCTTTGGAAA GTCGCATATA ACTGATGCAA AGGTAGATAG GATCAATCTT 
GTTATACTGG GTAAAGATGG ACTTGCTAGA GAATTAGCCA ATGAAATTCG GGCACTGTGT 
ACTAATGATG ATCATTATGT GCTTGAAGGA AGAATGTATG AGCTTGTCCT TCGGCCAATT 
GAGGGCAATG TTAGACTTCC TGTTAATTCG TTTCATACAT CCACATTTAC TCCCCATGGA 
TGCCTGTGTT TGTACAATTC AAAAGAGTCC TTGTCCTATG TTGTAGAAAG CATTGAGAAG 
TTGCGAGAGT CCACACTTGG CAGAAGGGAC AACCATATGG CTCAGCTTCC GTTGTCCCTC 
TTATTGGTAA GCAAAAGAGG TGTTGGAGGA CTGAGTGATA TTGGTGGTGA GACTGCTCAA 
GCGTTAATCT CACAGGGTCA GCAAGTAGCC ATAAAACTGC AGTGTAACTA CCTGGAACCT 
TCCTCCCCAG GCACTGGTTA TGGGCGAAAC GTGAATGAAA AGCAGATCAA CCAGGTTTTG 
AAAGCTCTAC TTGAAACAAG GAGGACTTCT ACATTTCGTA GCTGTTCTCC ACCTGCACCT 
CCACTGCCTC CATGTATTCG AGACTTTCAG CAAGACCAGA GCCCAGAGGC TGATTTGCGT 
ATCGTCATGT GCCTAATGTG TGGAGACTCT TATGACATCG ATCAACTTCT TGCACCATTC 
CTGCTGCCTC AGCATTGCAA GCCAACTTCT CCCCAGACAA GTGGCACTTC AGTGCTGCTA 
GAACAAACAA TTGGTGGCCA CAAGCTTACG ATTGAGCTTT CTCTGCTGTC TTACCACTCT 
TCTTTTGCTA CTCGGAAGAG TCGACTGGTG CATGGCTACA TAGCTGTATA TTCTGCTAGC 
CGCAAGGCTT CTTTTGAAAC CCTGTGTGCC TTTTTGTGTG AGGTTCAGGA CATCATCCCG 
GTACAGATGA TTGCAGTGGG GGAAACACAG ATTGAGCTTC AGGACTCTGA GTCTGCTCGT 
GAATTGTTAG TCCAAGGTGA AGAGCTGGCA CATGAACTTG AAGGACGATT TGGCAGTATT 
GTCTGTGGTC ATGGCGGCGT TGTTGGAGGA CTACACAAAC TAGACCTTTT AGAACAATTT 
TTCACAGAAG TATTAGAAAA GAAAACCATA GTTGAAGCCA GCCATATGTA TGAAAGCATG 
GCAGAAGCCA GTAGCACAAA TGAGAATGTG TATTCGCCAC ACTGTGGCTC CCCAAGCCCT 
GTCACAATGC TGTTAGATTC TGAGGAAGAC ATGGAAGCTT CCCCGCCTTA CCATGATGGC 
ACACTTACCT CTCACGGTGG ATTCAAACTG CCTGACCTGG ACTCAGGTGA CACATTCTCT 
GTGATCTCTG ACCTTAGCAC CTTTGAGAAC AAGCTAAATA ATAAAGTTCC TCCACAAGTG 
AGACCCAAAC CTAGTGTCAC ATTTGACCTT AGAAAGCCAA GTTTTAACCC CTATGTGGAT 
GGAGTCAGCC ACCGCCGTTC CCTCACATCC AATGTGACGT GGCCTCTAGG AGGAGATTAT 
GATCCTTCAG ACTATGCTGA GCCAATGGAT GCTGTCTCTA AGCCACGCCC CAGCTATGAA 
GACAGCATAT ATTCTGTGCC TCATGACAGC ACACAAGGTA AAATTATTAC TATACGCAAT 
TCAAATCGAA TCCACTCCAA CGGAGGAGGA AATGGTTCAG ACAGTGAAGG GGATGGCAGC 
TCATTGGAGA GACGTCGCAA GTTTTCAGCA GCAAGGGTTA AGCCACGGCT TTACCGTGAT 
CGTTCCAAAC GTCTGGGGAA GTTCAGCAGT TTCCGCACTA GTTTCTCTAT TGGTAGCGAT 
GATGAGATTG GCACACTCTC AAGGTCAAAA GAGGATGATG TTTCACCACT GAAGACAGAT 
ATAACAAATG AAGAAGGAGA AGATCCCAAA AAGAGAAACA TCCTGAAAAG TCTCCGCAGA 
CCAGGCAAAA AAGCAAGACC AAAGCCTCGT CATTCCATCT CTAAGCCTTT GGAGAGTAAC 
TACTTTGGAG TGCCTCTGGT CAATGTTGTT TCCTTCGAAC GACCTATTCC AGTTTTCATT 
GACAAGTGTA TTCGTTATAT TGAAGCCACA GGGTTAACAA CAGAGGGTAT CTACAGGGTA 
AGTGGGAATA AGGCGGAGAT CGAGGGCATG CAGAGGCAGT TTGAACAGGA CCACAATCTG 
GACTTTGTGG AGAAGGACTT CACTGTGAAC ACAGTTGCCG GGGCAATGAA AAGTTTCTTC 
TCTGAGCTGC CTGATCCTCT GGTTCCTTAC AGTTCACAGG AGGAGCTGGT GGAAGCTTTC 
AAAATTAATG ATCGAGAACA GAGACTTCAC ACAATGAAGG ACGTGTTAAG ACGATTTCCC 
AGGGAGAATT TTGATGTCTT CAAGTATGTA ATGAGCCATC TAAACAAGGT TGGTCAGTGG 
AACAGAGTCA ACTTAATGAC CAGTGAGAAT CTCTCCATCT GTTTCTGGCC AACACTGATG 
CGGCCAGACT TCACTTCTAT GGATGCCCTG ACCGCCACAC GCACCTACCA GACAATCATC 
GAGACGTTTA TCCATCAATG TGCTTTCTTC TTTTACAACC AGCCTCCTGC TGAATCGCCC 
ACTGGCCTAT TCGGCCAGCC TGGACCAATG GTGCCCTGTG GATTGCCCCC ATCCCCCACA 
GCTGCTCCTT CTTATTGCCC TTCGACCCCA CATTTTACCC CTCTTCTTCA GTCCCCGCCT 
CATTCTCCCC CCCAGAGCCC ACACTCCGCC CCTGCAGAAC CACAAACACT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009299965.1
CDS	1268..5821
Translation	MMMAKKQESRLPIYNLVVVGLSGTEKEKGQCGVGKSCLCNRFVRPSADDFHLDHTSVLSTSDFGGRVVNNDHFLFWGEVGRVLEDGADCRMHVVEQTEFIDDQTFQPHRSTALLPYIKRAASTKLASAEKLMYFCTDQLGLEQDFEQKQMPEGKLQVDGFLLCVDVSRGMNRNFDDQMKFVTNLYNHLSKTKKPIVLVLTKCDEGVERYIKDSHTFAITKKNLQVVETSARSNINVDLAFLTLIQLIDKGKGKPKIIPYFEALKHQSQLIASAKDRYEWLVNHIVKNHNETWPNVSRRMQTSPEYKEYVFLEGTGKAKKLFQQHIHRLKQEHTERRRKAYLSALPLALSSLVSELDEIEHLSWSGVQKVLESKKDFDHWFVVLDDAPWEDTPHLDNMEDDRIPFDVLDTTAAEILFDAHLEHLRNECKRAQMRHEFKGKLASSPFVTPGKPWEEARSFIMNEDFYQWLEEPEYLDIYNRHQKAIIDRAKEDFQELLLEYSELFYELEVDAKPSKEKMGAIQEVLGEEQRFKALQKLQAERDALVLKHIHFVYHPTKETCPNCPHCVDSKIEQILASCFPAQYPSFGKSHITDAKVDRINLVILGKDGLARELANEIRALCTNDDHYVLEGRMYELVLRPIEGNVRLPVNSFHTSTFTPHGCLCLYNSKESLSYVVESIEKLRESTLGRRDNHMAQLPLSLLLVSKRGVGGLSDIGGETAQALISQGQQVAIKLQCNYLEPSSPGTGYGRNVNEKQINQVLKALLETRRTSTFRSCSPPAPPLPPCIRDFQQDQSPEADLRIVMCLMCGDSYDIDQLLAPFLLPQHCKPTSPQTSGTSVLLEQTIGGHKLTIELSLLSYHSSFATRKSRLVHGYIAVYSASRKASFETLCAFLCEVQDIIPVQMIAVGETQIELQDSESARELLVQGEELAHELEGRFGSIVCGHGGVVGGLHKLDLLEQFFTEVLEKKTIVEASHMYESMAEASSTNENVYSPHCGSPSPVTMLLDSEEDMEASPPYHDGTLTSHGGFKLPDLDSGDTFSVISDLSTFENKLNNKVPPQVRPKPSVTFDLRKPSFNPYVDGVSHRRSLTSNVTWPLGGDYDPSDYAEPMDAVSKPRPSYEDSIYSVPHDSTQGKIITIRNSNRIHSNGGGNGSDSEGDGSSLERRRKFSAARVKPRLYRDRSKRLGKFSSFRTSFSIGSDDEIGTLSRSKEDDVSPLKTDITNEEGEDPKKRNILKSLRRPGKKARPKPRHSISKPLESNYFGVPLVNVVSFERPIPVFIDKCIRYIEATGLTTEGIYRVSGNKAEIEGMQRQFEQDHNLDFVEKDFTVNTVAGAMKSFFSELPDPLVPYSSQEELVEAFKINDREQRLHTMKDVLRRFPRENFDVFKYVMSHLNKVGQWNRVNLMTSENLSICFWPTLMRPDFTSMDALTATRTYQTIIETFIHQCAFFFYNQPPAESPTGLFGQPGPMVPCGLPPSPTAAPSYCPSTPHFTPLLQSPPHSPPQSPHSAPAEPQTL

Target ORF information:

RefSeq Version	XM_009301690.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Rho GTPase activating protein 35b (arhgap35), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301690.3

ORF Insert Sequence:

ATGATGATGG CTAAAAAGCA AGAATCCCGG CTGCCAATCT ACAACCTTGT TGTTGTTGGC 
CTGTCGGGAA CTGAGAAGGA GAAAGGGCAA TGTGGAGTTG GCAAATCTTG CCTTTGTAAT 
CGCTTTGTTC GCCCTAGTGC AGATGACTTC CATCTCGATC ACACCTCTGT GCTAAGTACT 
AGTGACTTTG GTGGCCGGGT TGTCAACAAT GACCATTTCC TGTTCTGGGG GGAGGTTGGG 
AGAGTTTTGG AGGATGGTGC AGACTGCAGA ATGCATGTGG TCGAGCAGAC AGAATTTATT 
GATGACCAGA CTTTCCAGCC CCATCGTAGC ACAGCCTTAC TGCCCTACAT CAAAAGAGCT 
GCCTCCACCA AATTGGCTTC TGCGGAAAAA CTCATGTACT TCTGCACTGA TCAGTTGGGC 
CTGGAGCAGG ACTTCGAACA GAAACAAATG CCTGAAGGCA AGTTGCAGGT CGATGGCTTT 
CTGCTTTGTG TTGATGTCAG TCGTGGTATG AACCGCAACT TTGATGATCA AATGAAATTT 
GTCACCAACT TGTACAATCA CTTAAGCAAG ACAAAAAAGC CTATTGTGCT GGTTCTCACC 
AAGTGTGATG AGGGTGTGGA GCGATACATC AAGGATTCTC ATACCTTTGC CATTACAAAA 
AAGAACCTGC AAGTTGTTGA GACTTCGGCT CGATCCAATA TCAATGTTGA CTTGGCTTTT 
CTTACCTTAA TTCAACTCAT TGATAAGGGA AAAGGTAAGC CTAAGATCAT ACCTTACTTT 
GAGGCATTGA AACACCAGAG TCAACTGATT GCCTCCGCAA AAGACCGCTA TGAATGGTTG 
GTGAACCACA TTGTGAAGAA TCACAATGAA ACTTGGCCAA ACGTCAGCAG ACGTATGCAG 
ACTTCACCCG AATATAAGGA ATATGTGTTT TTAGAAGGCA CAGGTAAAGC TAAGAAACTA 
TTTCAACAGC ACATTCACCG ACTTAAACAA GAGCATACTG AAAGACGTCG TAAAGCATAC 
TTGAGTGCTC TTCCACTCGC CTTAAGTAGC TTGGTGTCGG AGTTGGATGA AATTGAGCAT 
TTAAGCTGGT CTGGGGTGCA AAAAGTGCTT GAGTCTAAAA AAGACTTTGA TCATTGGTTT 
GTGGTACTGG ACGATGCACC ATGGGAAGAC ACACCACATC TTGACAATAT GGAGGATGAC 
CGCATCCCCT TTGATGTGCT AGACACCACG GCTGCTGAAA TCCTCTTTGA TGCACACCTT 
GAACACTTGC GCAATGAATG CAAGCGTGCC CAGATGCGTC ATGAGTTCAA AGGAAAATTA 
GCATCTTCAC CTTTCGTTAC TCCAGGCAAG CCCTGGGAGG AGGCTCGAAG CTTCATCATG 
AATGAAGACT TTTACCAGTG GCTTGAGGAG CCAGAATATT TGGACATTTA TAACCGTCAC 
CAGAAAGCAA TCATTGACAG GGCAAAAGAG GATTTTCAGG AGCTTCTGCT GGAGTACTCT 
GAACTCTTCT ACGAACTTGA GGTGGATGCC AAACCTAGTA AAGAGAAAAT GGGAGCAATT 
CAAGAAGTGT TAGGTGAAGA ACAACGCTTC AAGGCACTTC AGAAACTGCA AGCTGAAAGG 
GATGCTTTAG TACTGAAACA CATCCATTTT GTTTATCACC CTACGAAGGA AACATGCCCA 
AACTGCCCAC ACTGTGTTGA CAGCAAAATT GAGCAAATTC TAGCTTCATG CTTCCCTGCA 
CAATATCCTT CCTTTGGAAA GTCGCATATA ACTGATGCAA AGGTAGATAG GATCAATCTT 
GTTATACTGG GTAAAGATGG ACTTGCTAGA GAATTAGCCA ATGAAATTCG GGCACTGTGT 
ACTAATGATG ATCATTATGT GCTTGAAGGA AGAATGTATG AGCTTGTCCT TCGGCCAATT 
GAGGGCAATG TTAGACTTCC TGTTAATTCG TTTCATACAT CCACATTTAC TCCCCATGGA 
TGCCTGTGTT TGTACAATTC AAAAGAGTCC TTGTCCTATG TTGTAGAAAG CATTGAGAAG 
TTGCGAGAGT CCACACTTGG CAGAAGGGAC AACCATATGG CTCAGCTTCC GTTGTCCCTC 
TTATTGGTAA GCAAAAGAGG TGTTGGAGGA CTGAGTGATA TTGGTGGTGA GACTGCTCAA 
GCGTTAATCT CACAGGGTCA GCAAGTAGCC ATAAAACTGC AGTGTAACTA CCTGGAACCT 
TCCTCCCCAG GCACTGGTTA TGGGCGAAAC GTGAATGAAA AGCAGATCAA CCAGGTTTTG 
AAAGCTCTAC TTGAAACAAG GAGGACTTCT ACATTTCGTA GCTGTTCTCC ACCTGCACCT 
CCACTGCCTC CATGTATTCG AGACTTTCAG CAAGACCAGA GCCCAGAGGC TGATTTGCGT 
ATCGTCATGT GCCTAATGTG TGGAGACTCT TATGACATCG ATCAACTTCT TGCACCATTC 
CTGCTGCCTC AGCATTGCAA GCCAACTTCT CCCCAGACAA GTGGCACTTC AGTGCTGCTA 
GAACAAACAA TTGGTGGCCA CAAGCTTACG ATTGAGCTTT CTCTGCTGTC TTACCACTCT 
TCTTTTGCTA CTCGGAAGAG TCGACTGGTG CATGGCTACA TAGCTGTATA TTCTGCTAGC 
CGCAAGGCTT CTTTTGAAAC CCTGTGTGCC TTTTTGTGTG AGGTTCAGGA CATCATCCCG 
GTACAGATGA TTGCAGTGGG GGAAACACAG ATTGAGCTTC AGGACTCTGA GTCTGCTCGT 
GAATTGTTAG TCCAAGGTGA AGAGCTGGCA CATGAACTTG AAGGACGATT TGGCAGTATT 
GTCTGTGGTC ATGGCGGCGT TGTTGGAGGA CTACACAAAC TAGACCTTTT AGAACAATTT 
TTCACAGAAG TATTAGAAAA GAAAACCATA GTTGAAGCCA GCCATATGTA TGAAAGCATG 
GCAGAAGCCA GTAGCACAAA TGAGAATGTG TATTCGCCAC ACTGTGGCTC CCCAAGCCCT 
GTCACAATGC TGTTAGATTC TGAGGAAGAC ATGGAAGCTT CCCCGCCTTA CCATGATGGC 
ACACTTACCT CTCACGGTGG ATTCAAACTG CCTGACCTGG ACTCAGGTGA CACATTCTCT 
GTGATCTCTG ACCTTAGCAC CTTTGAGAAC AAGCTAAATA ATAAAGTTCC TCCACAAGTG 
AGACCCAAAC CTAGTGTCAC ATTTGACCTT AGAAAGCCAA GTTTTAACCC CTATGTGGAT 
GGAGTCAGCC ACCGCCGTTC CCTCACATCC AATGTGACGT GGCCTCTAGG AGGAGATTAT 
GATCCTTCAG ACTATGCTGA GCCAATGGAT GCTGTCTCTA AGCCACGCCC CAGCTATGAA 
GACAGCATAT ATTCTGTGCC TCATGACAGC ACACAAGGTA AAATTATTAC TATACGCAAT 
TCAAATCGAA TCCACTCCAA CGGAGGAGGA AATGGTTCAG ACAGTGAAGG GGATGGCAGC 
TCATTGGAGA GACGTCGCAA GTTTTCAGCA GCAAGGGTTA AGCCACGGCT TTACCGTGAT 
CGTTCCAAAC GTCTGGGGAA GTTCAGCAGT TTCCGCACTA GTTTCTCTAT TGGTAGCGAT 
GATGAGATTG GCACACTCTC AAGGTCAAAA GAGGATGATG TTTCACCACT GAAGACAGAT 
ATAACAAATG AAGAAGGAGA AGATCCCAAA AAGAGAAACA TCCTGAAAAG TCTCCGCAGA 
CCAGGCAAAA AAGCAAGACC AAAGCCTCGT CATTCCATCT CTAAGCCTTT GGAGAGTAAC 
TACTTTGGAG TGCCTCTGGT CAATGTTGTT TCCTTCGAAC GACCTATTCC AGTTTTCATT 
GACAAGTGTA TTCGTTATAT TGAAGCCACA GGGTTAACAA CAGAGGGTAT CTACAGGGTA 
AGTGGGAATA AGGCGGAGAT CGAGGGCATG CAGAGGCAGT TTGAACAGGA CCACAATCTG 
GACTTTGTGG AGAAGGACTT CACTGTGAAC ACAGTTGCCG GGGCAATGAA AAGTTTCTTC 
TCTGAGCTGC CTGATCCTCT GGTTCCTTAC AGTTCACAGG AGGAGCTGGT GGAAGCTTTC 
AAAATTAATG ATCGAGAACA GAGACTTCAC ACAATGAAGG ACGTGTTAAG ACGATTTCCC 
AGGGAGAATT TTGATGTCTT CAAGTATGTA ATGAGCCATC TAAACAAGGT TGGTCAGTGG 
AACAGAGTCA ACTTAATGAC CAGTGAGAAT CTCTCCATCT GTTTCTGGCC AACACTGATG 
CGGCCAGACT TCACTTCTAT GGATGCCCTG ACCGCCACAC GCACCTACCA GACAATCATC 
GAGACGTTTA TCCATCAATG TGCTTTCTTC TTTTACAACC AGCCTCCTGC TGAATCGCCC 
ACTGGCCTAT TCGGCCAGCC TGGACCAATG GTGCCCTGTG GATTGCCCCC ATCCCCCACA 
GCTGCTCCTT CTTATTGCCC TTCGACCCCA CATTTTACCC CTCTTCTTCA GTCCCCGCCT 
CATTCTCCCC CCCAGAGCCC ACACTCCGCC CCTGCAGAAC CACAAACACT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgap35 ( XM_021476553.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021332228.1 Danio rerio Rho GTPase activating protein 35b (arhgap35), transcript variant X1, mRNA.

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CloneID	ODa44355
Clone ID Related Accession (Same CDS sequence)	XM_003198643.5 , XM_009301690.3 , XM_021476553.1 , XM_009301690.2 , XM_003198643.4 , XM_021476554.1
Accession Version	XM_021476553.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4554bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	rho GTPase-activating protein 35
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGG CTAAAAAGCA AGAATCCCGG CTGCCAATCT ACAACCTTGT TGTTGTTGGC 
CTGTCGGGAA CTGAGAAGGA GAAAGGGCAA TGTGGAGTTG GCAAATCTTG CCTTTGTAAT 
CGCTTTGTTC GCCCTAGTGC AGATGACTTC CATCTCGATC ACACCTCTGT GCTAAGTACT 
AGTGACTTTG GTGGCCGGGT TGTCAACAAT GACCATTTCC TGTTCTGGGG GGAGGTTGGG 
AGAGTTTTGG AGGATGGTGC AGACTGCAGA ATGCATGTGG TCGAGCAGAC AGAATTTATT 
GATGACCAGA CTTTCCAGCC CCATCGTAGC ACAGCCTTAC TGCCCTACAT CAAAAGAGCT 
GCCTCCACCA AATTGGCTTC TGCGGAAAAA CTCATGTACT TCTGCACTGA TCAGTTGGGC 
CTGGAGCAGG ACTTCGAACA GAAACAAATG CCTGAAGGCA AGTTGCAGGT CGATGGCTTT 
CTGCTTTGTG TTGATGTCAG TCGTGGTATG AACCGCAACT TTGATGATCA AATGAAATTT 
GTCACCAACT TGTACAATCA CTTAAGCAAG ACAAAAAAGC CTATTGTGCT GGTTCTCACC 
AAGTGTGATG AGGGTGTGGA GCGATACATC AAGGATTCTC ATACCTTTGC CATTACAAAA 
AAGAACCTGC AAGTTGTTGA GACTTCGGCT CGATCCAATA TCAATGTTGA CTTGGCTTTT 
CTTACCTTAA TTCAACTCAT TGATAAGGGA AAAGGTAAGC CTAAGATCAT ACCTTACTTT 
GAGGCATTGA AACACCAGAG TCAACTGATT GCCTCCGCAA AAGACCGCTA TGAATGGTTG 
GTGAACCACA TTGTGAAGAA TCACAATGAA ACTTGGCCAA ACGTCAGCAG ACGTATGCAG 
ACTTCACCCG AATATAAGGA ATATGTGTTT TTAGAAGGCA CAGGTAAAGC TAAGAAACTA 
TTTCAACAGC ACATTCACCG ACTTAAACAA GAGCATACTG AAAGACGTCG TAAAGCATAC 
TTGAGTGCTC TTCCACTCGC CTTAAGTAGC TTGGTGTCGG AGTTGGATGA AATTGAGCAT 
TTAAGCTGGT CTGGGGTGCA AAAAGTGCTT GAGTCTAAAA AAGACTTTGA TCATTGGTTT 
GTGGTACTGG ACGATGCACC ATGGGAAGAC ACACCACATC TTGACAATAT GGAGGATGAC 
CGCATCCCCT TTGATGTGCT AGACACCACG GCTGCTGAAA TCCTCTTTGA TGCACACCTT 
GAACACTTGC GCAATGAATG CAAGCGTGCC CAGATGCGTC ATGAGTTCAA AGGAAAATTA 
GCATCTTCAC CTTTCGTTAC TCCAGGCAAG CCCTGGGAGG AGGCTCGAAG CTTCATCATG 
AATGAAGACT TTTACCAGTG GCTTGAGGAG CCAGAATATT TGGACATTTA TAACCGTCAC 
CAGAAAGCAA TCATTGACAG GGCAAAAGAG GATTTTCAGG AGCTTCTGCT GGAGTACTCT 
GAACTCTTCT ACGAACTTGA GGTGGATGCC AAACCTAGTA AAGAGAAAAT GGGAGCAATT 
CAAGAAGTGT TAGGTGAAGA ACAACGCTTC AAGGCACTTC AGAAACTGCA AGCTGAAAGG 
GATGCTTTAG TACTGAAACA CATCCATTTT GTTTATCACC CTACGAAGGA AACATGCCCA 
AACTGCCCAC ACTGTGTTGA CAGCAAAATT GAGCAAATTC TAGCTTCATG CTTCCCTGCA 
CAATATCCTT CCTTTGGAAA GTCGCATATA ACTGATGCAA AGGTAGATAG GATCAATCTT 
GTTATACTGG GTAAAGATGG ACTTGCTAGA GAATTAGCCA ATGAAATTCG GGCACTGTGT 
ACTAATGATG ATCATTATGT GCTTGAAGGA AGAATGTATG AGCTTGTCCT TCGGCCAATT 
GAGGGCAATG TTAGACTTCC TGTTAATTCG TTTCATACAT CCACATTTAC TCCCCATGGA 
TGCCTGTGTT TGTACAATTC AAAAGAGTCC TTGTCCTATG TTGTAGAAAG CATTGAGAAG 
TTGCGAGAGT CCACACTTGG CAGAAGGGAC AACCATATGG CTCAGCTTCC GTTGTCCCTC 
TTATTGGTAA GCAAAAGAGG TGTTGGAGGA CTGAGTGATA TTGGTGGTGA GACTGCTCAA 
GCGTTAATCT CACAGGGTCA GCAAGTAGCC ATAAAACTGC AGTGTAACTA CCTGGAACCT 
TCCTCCCCAG GCACTGGTTA TGGGCGAAAC GTGAATGAAA AGCAGATCAA CCAGGTTTTG 
AAAGCTCTAC TTGAAACAAG GAGGACTTCT ACATTTCGTA GCTGTTCTCC ACCTGCACCT 
CCACTGCCTC CATGTATTCG AGACTTTCAG CAAGACCAGA GCCCAGAGGC TGATTTGCGT 
ATCGTCATGT GCCTAATGTG TGGAGACTCT TATGACATCG ATCAACTTCT TGCACCATTC 
CTGCTGCCTC AGCATTGCAA GCCAACTTCT CCCCAGACAA GTGGCACTTC AGTGCTGCTA 
GAACAAACAA TTGGTGGCCA CAAGCTTACG ATTGAGCTTT CTCTGCTGTC TTACCACTCT 
TCTTTTGCTA CTCGGAAGAG TCGACTGGTG CATGGCTACA TAGCTGTATA TTCTGCTAGC 
CGCAAGGCTT CTTTTGAAAC CCTGTGTGCC TTTTTGTGTG AGGTTCAGGA CATCATCCCG 
GTACAGATGA TTGCAGTGGG GGAAACACAG ATTGAGCTTC AGGACTCTGA GTCTGCTCGT 
GAATTGTTAG TCCAAGGTGA AGAGCTGGCA CATGAACTTG AAGGACGATT TGGCAGTATT 
GTCTGTGGTC ATGGCGGCGT TGTTGGAGGA CTACACAAAC TAGACCTTTT AGAACAATTT 
TTCACAGAAG TATTAGAAAA GAAAACCATA GTTGAAGCCA GCCATATGTA TGAAAGCATG 
GCAGAAGCCA GTAGCACAAA TGAGAATGTG TATTCGCCAC ACTGTGGCTC CCCAAGCCCT 
GTCACAATGC TGTTAGATTC TGAGGAAGAC ATGGAAGCTT CCCCGCCTTA CCATGATGGC 
ACACTTACCT CTCACGGTGG ATTCAAACTG CCTGACCTGG ACTCAGGTGA CACATTCTCT 
GTGATCTCTG ACCTTAGCAC CTTTGAGAAC AAGCTAAATA ATAAAGTTCC TCCACAAGTG 
AGACCCAAAC CTAGTGTCAC ATTTGACCTT AGAAAGCCAA GTTTTAACCC CTATGTGGAT 
GGAGTCAGCC ACCGCCGTTC CCTCACATCC AATGTGACGT GGCCTCTAGG AGGAGATTAT 
GATCCTTCAG ACTATGCTGA GCCAATGGAT GCTGTCTCTA AGCCACGCCC CAGCTATGAA 
GACAGCATAT ATTCTGTGCC TCATGACAGC ACACAAGGTA AAATTATTAC TATACGCAAT 
TCAAATCGAA TCCACTCCAA CGGAGGAGGA AATGGTTCAG ACAGTGAAGG GGATGGCAGC 
TCATTGGAGA GACGTCGCAA GTTTTCAGCA GCAAGGGTTA AGCCACGGCT TTACCGTGAT 
CGTTCCAAAC GTCTGGGGAA GTTCAGCAGT TTCCGCACTA GTTTCTCTAT TGGTAGCGAT 
GATGAGATTG GCACACTCTC AAGGTCAAAA GAGGATGATG TTTCACCACT GAAGACAGAT 
ATAACAAATG AAGAAGGAGA AGATCCCAAA AAGAGAAACA TCCTGAAAAG TCTCCGCAGA 
CCAGGCAAAA AAGCAAGACC AAAGCCTCGT CATTCCATCT CTAAGCCTTT GGAGAGTAAC 
TACTTTGGAG TGCCTCTGGT CAATGTTGTT TCCTTCGAAC GACCTATTCC AGTTTTCATT 
GACAAGTGTA TTCGTTATAT TGAAGCCACA GGGTTAACAA CAGAGGGTAT CTACAGGGTA 
AGTGGGAATA AGGCGGAGAT CGAGGGCATG CAGAGGCAGT TTGAACAGGA CCACAATCTG 
GACTTTGTGG AGAAGGACTT CACTGTGAAC ACAGTTGCCG GGGCAATGAA AAGTTTCTTC 
TCTGAGCTGC CTGATCCTCT GGTTCCTTAC AGTTCACAGG AGGAGCTGGT GGAAGCTTTC 
AAAATTAATG ATCGAGAACA GAGACTTCAC ACAATGAAGG ACGTGTTAAG ACGATTTCCC 
AGGGAGAATT TTGATGTCTT CAAGTATGTA ATGAGCCATC TAAACAAGGT TGGTCAGTGG 
AACAGAGTCA ACTTAATGAC CAGTGAGAAT CTCTCCATCT GTTTCTGGCC AACACTGATG 
CGGCCAGACT TCACTTCTAT GGATGCCCTG ACCGCCACAC GCACCTACCA GACAATCATC 
GAGACGTTTA TCCATCAATG TGCTTTCTTC TTTTACAACC AGCCTCCTGC TGAATCGCCC 
ACTGGCCTAT TCGGCCAGCC TGGACCAATG GTGCCCTGTG GATTGCCCCC ATCCCCCACA 
GCTGCTCCTT CTTATTGCCC TTCGACCCCA CATTTTACCC CTCTTCTTCA GTCCCCGCCT 
CATTCTCCCC CCCAGAGCCC ACACTCCGCC CCTGCAGAAC CACAAACACT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021332228.1
CDS	1264..5817
Translation	MMMAKKQESRLPIYNLVVVGLSGTEKEKGQCGVGKSCLCNRFVRPSADDFHLDHTSVLSTSDFGGRVVNNDHFLFWGEVGRVLEDGADCRMHVVEQTEFIDDQTFQPHRSTALLPYIKRAASTKLASAEKLMYFCTDQLGLEQDFEQKQMPEGKLQVDGFLLCVDVSRGMNRNFDDQMKFVTNLYNHLSKTKKPIVLVLTKCDEGVERYIKDSHTFAITKKNLQVVETSARSNINVDLAFLTLIQLIDKGKGKPKIIPYFEALKHQSQLIASAKDRYEWLVNHIVKNHNETWPNVSRRMQTSPEYKEYVFLEGTGKAKKLFQQHIHRLKQEHTERRRKAYLSALPLALSSLVSELDEIEHLSWSGVQKVLESKKDFDHWFVVLDDAPWEDTPHLDNMEDDRIPFDVLDTTAAEILFDAHLEHLRNECKRAQMRHEFKGKLASSPFVTPGKPWEEARSFIMNEDFYQWLEEPEYLDIYNRHQKAIIDRAKEDFQELLLEYSELFYELEVDAKPSKEKMGAIQEVLGEEQRFKALQKLQAERDALVLKHIHFVYHPTKETCPNCPHCVDSKIEQILASCFPAQYPSFGKSHITDAKVDRINLVILGKDGLARELANEIRALCTNDDHYVLEGRMYELVLRPIEGNVRLPVNSFHTSTFTPHGCLCLYNSKESLSYVVESIEKLRESTLGRRDNHMAQLPLSLLLVSKRGVGGLSDIGGETAQALISQGQQVAIKLQCNYLEPSSPGTGYGRNVNEKQINQVLKALLETRRTSTFRSCSPPAPPLPPCIRDFQQDQSPEADLRIVMCLMCGDSYDIDQLLAPFLLPQHCKPTSPQTSGTSVLLEQTIGGHKLTIELSLLSYHSSFATRKSRLVHGYIAVYSASRKASFETLCAFLCEVQDIIPVQMIAVGETQIELQDSESARELLVQGEELAHELEGRFGSIVCGHGGVVGGLHKLDLLEQFFTEVLEKKTIVEASHMYESMAEASSTNENVYSPHCGSPSPVTMLLDSEEDMEASPPYHDGTLTSHGGFKLPDLDSGDTFSVISDLSTFENKLNNKVPPQVRPKPSVTFDLRKPSFNPYVDGVSHRRSLTSNVTWPLGGDYDPSDYAEPMDAVSKPRPSYEDSIYSVPHDSTQGKIITIRNSNRIHSNGGGNGSDSEGDGSSLERRRKFSAARVKPRLYRDRSKRLGKFSSFRTSFSIGSDDEIGTLSRSKEDDVSPLKTDITNEEGEDPKKRNILKSLRRPGKKARPKPRHSISKPLESNYFGVPLVNVVSFERPIPVFIDKCIRYIEATGLTTEGIYRVSGNKAEIEGMQRQFEQDHNLDFVEKDFTVNTVAGAMKSFFSELPDPLVPYSSQEELVEAFKINDREQRLHTMKDVLRRFPRENFDVFKYVMSHLNKVGQWNRVNLMTSENLSICFWPTLMRPDFTSMDALTATRTYQTIIETFIHQCAFFFYNQPPAESPTGLFGQPGPMVPCGLPPSPTAAPSYCPSTPHFTPLLQSPPHSPPQSPHSAPAEPQTL

Target ORF information:

RefSeq Version	XM_021476553.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Rho GTPase activating protein 35b (arhgap35), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476553.1

ORF Insert Sequence:

ATGATGATGG CTAAAAAGCA AGAATCCCGG CTGCCAATCT ACAACCTTGT TGTTGTTGGC 
CTGTCGGGAA CTGAGAAGGA GAAAGGGCAA TGTGGAGTTG GCAAATCTTG CCTTTGTAAT 
CGCTTTGTTC GCCCTAGTGC AGATGACTTC CATCTCGATC ACACCTCTGT GCTAAGTACT 
AGTGACTTTG GTGGCCGGGT TGTCAACAAT GACCATTTCC TGTTCTGGGG GGAGGTTGGG 
AGAGTTTTGG AGGATGGTGC AGACTGCAGA ATGCATGTGG TCGAGCAGAC AGAATTTATT 
GATGACCAGA CTTTCCAGCC CCATCGTAGC ACAGCCTTAC TGCCCTACAT CAAAAGAGCT 
GCCTCCACCA AATTGGCTTC TGCGGAAAAA CTCATGTACT TCTGCACTGA TCAGTTGGGC 
CTGGAGCAGG ACTTCGAACA GAAACAAATG CCTGAAGGCA AGTTGCAGGT CGATGGCTTT 
CTGCTTTGTG TTGATGTCAG TCGTGGTATG AACCGCAACT TTGATGATCA AATGAAATTT 
GTCACCAACT TGTACAATCA CTTAAGCAAG ACAAAAAAGC CTATTGTGCT GGTTCTCACC 
AAGTGTGATG AGGGTGTGGA GCGATACATC AAGGATTCTC ATACCTTTGC CATTACAAAA 
AAGAACCTGC AAGTTGTTGA GACTTCGGCT CGATCCAATA TCAATGTTGA CTTGGCTTTT 
CTTACCTTAA TTCAACTCAT TGATAAGGGA AAAGGTAAGC CTAAGATCAT ACCTTACTTT 
GAGGCATTGA AACACCAGAG TCAACTGATT GCCTCCGCAA AAGACCGCTA TGAATGGTTG 
GTGAACCACA TTGTGAAGAA TCACAATGAA ACTTGGCCAA ACGTCAGCAG ACGTATGCAG 
ACTTCACCCG AATATAAGGA ATATGTGTTT TTAGAAGGCA CAGGTAAAGC TAAGAAACTA 
TTTCAACAGC ACATTCACCG ACTTAAACAA GAGCATACTG AAAGACGTCG TAAAGCATAC 
TTGAGTGCTC TTCCACTCGC CTTAAGTAGC TTGGTGTCGG AGTTGGATGA AATTGAGCAT 
TTAAGCTGGT CTGGGGTGCA AAAAGTGCTT GAGTCTAAAA AAGACTTTGA TCATTGGTTT 
GTGGTACTGG ACGATGCACC ATGGGAAGAC ACACCACATC TTGACAATAT GGAGGATGAC 
CGCATCCCCT TTGATGTGCT AGACACCACG GCTGCTGAAA TCCTCTTTGA TGCACACCTT 
GAACACTTGC GCAATGAATG CAAGCGTGCC CAGATGCGTC ATGAGTTCAA AGGAAAATTA 
GCATCTTCAC CTTTCGTTAC TCCAGGCAAG CCCTGGGAGG AGGCTCGAAG CTTCATCATG 
AATGAAGACT TTTACCAGTG GCTTGAGGAG CCAGAATATT TGGACATTTA TAACCGTCAC 
CAGAAAGCAA TCATTGACAG GGCAAAAGAG GATTTTCAGG AGCTTCTGCT GGAGTACTCT 
GAACTCTTCT ACGAACTTGA GGTGGATGCC AAACCTAGTA AAGAGAAAAT GGGAGCAATT 
CAAGAAGTGT TAGGTGAAGA ACAACGCTTC AAGGCACTTC AGAAACTGCA AGCTGAAAGG 
GATGCTTTAG TACTGAAACA CATCCATTTT GTTTATCACC CTACGAAGGA AACATGCCCA 
AACTGCCCAC ACTGTGTTGA CAGCAAAATT GAGCAAATTC TAGCTTCATG CTTCCCTGCA 
CAATATCCTT CCTTTGGAAA GTCGCATATA ACTGATGCAA AGGTAGATAG GATCAATCTT 
GTTATACTGG GTAAAGATGG ACTTGCTAGA GAATTAGCCA ATGAAATTCG GGCACTGTGT 
ACTAATGATG ATCATTATGT GCTTGAAGGA AGAATGTATG AGCTTGTCCT TCGGCCAATT 
GAGGGCAATG TTAGACTTCC TGTTAATTCG TTTCATACAT CCACATTTAC TCCCCATGGA 
TGCCTGTGTT TGTACAATTC AAAAGAGTCC TTGTCCTATG TTGTAGAAAG CATTGAGAAG 
TTGCGAGAGT CCACACTTGG CAGAAGGGAC AACCATATGG CTCAGCTTCC GTTGTCCCTC 
TTATTGGTAA GCAAAAGAGG TGTTGGAGGA CTGAGTGATA TTGGTGGTGA GACTGCTCAA 
GCGTTAATCT CACAGGGTCA GCAAGTAGCC ATAAAACTGC AGTGTAACTA CCTGGAACCT 
TCCTCCCCAG GCACTGGTTA TGGGCGAAAC GTGAATGAAA AGCAGATCAA CCAGGTTTTG 
AAAGCTCTAC TTGAAACAAG GAGGACTTCT ACATTTCGTA GCTGTTCTCC ACCTGCACCT 
CCACTGCCTC CATGTATTCG AGACTTTCAG CAAGACCAGA GCCCAGAGGC TGATTTGCGT 
ATCGTCATGT GCCTAATGTG TGGAGACTCT TATGACATCG ATCAACTTCT TGCACCATTC 
CTGCTGCCTC AGCATTGCAA GCCAACTTCT CCCCAGACAA GTGGCACTTC AGTGCTGCTA 
GAACAAACAA TTGGTGGCCA CAAGCTTACG ATTGAGCTTT CTCTGCTGTC TTACCACTCT 
TCTTTTGCTA CTCGGAAGAG TCGACTGGTG CATGGCTACA TAGCTGTATA TTCTGCTAGC 
CGCAAGGCTT CTTTTGAAAC CCTGTGTGCC TTTTTGTGTG AGGTTCAGGA CATCATCCCG 
GTACAGATGA TTGCAGTGGG GGAAACACAG ATTGAGCTTC AGGACTCTGA GTCTGCTCGT 
GAATTGTTAG TCCAAGGTGA AGAGCTGGCA CATGAACTTG AAGGACGATT TGGCAGTATT 
GTCTGTGGTC ATGGCGGCGT TGTTGGAGGA CTACACAAAC TAGACCTTTT AGAACAATTT 
TTCACAGAAG TATTAGAAAA GAAAACCATA GTTGAAGCCA GCCATATGTA TGAAAGCATG 
GCAGAAGCCA GTAGCACAAA TGAGAATGTG TATTCGCCAC ACTGTGGCTC CCCAAGCCCT 
GTCACAATGC TGTTAGATTC TGAGGAAGAC ATGGAAGCTT CCCCGCCTTA CCATGATGGC 
ACACTTACCT CTCACGGTGG ATTCAAACTG CCTGACCTGG ACTCAGGTGA CACATTCTCT 
GTGATCTCTG ACCTTAGCAC CTTTGAGAAC AAGCTAAATA ATAAAGTTCC TCCACAAGTG 
AGACCCAAAC CTAGTGTCAC ATTTGACCTT AGAAAGCCAA GTTTTAACCC CTATGTGGAT 
GGAGTCAGCC ACCGCCGTTC CCTCACATCC AATGTGACGT GGCCTCTAGG AGGAGATTAT 
GATCCTTCAG ACTATGCTGA GCCAATGGAT GCTGTCTCTA AGCCACGCCC CAGCTATGAA 
GACAGCATAT ATTCTGTGCC TCATGACAGC ACACAAGGTA AAATTATTAC TATACGCAAT 
TCAAATCGAA TCCACTCCAA CGGAGGAGGA AATGGTTCAG ACAGTGAAGG GGATGGCAGC 
TCATTGGAGA GACGTCGCAA GTTTTCAGCA GCAAGGGTTA AGCCACGGCT TTACCGTGAT 
CGTTCCAAAC GTCTGGGGAA GTTCAGCAGT TTCCGCACTA GTTTCTCTAT TGGTAGCGAT 
GATGAGATTG GCACACTCTC AAGGTCAAAA GAGGATGATG TTTCACCACT GAAGACAGAT 
ATAACAAATG AAGAAGGAGA AGATCCCAAA AAGAGAAACA TCCTGAAAAG TCTCCGCAGA 
CCAGGCAAAA AAGCAAGACC AAAGCCTCGT CATTCCATCT CTAAGCCTTT GGAGAGTAAC 
TACTTTGGAG TGCCTCTGGT CAATGTTGTT TCCTTCGAAC GACCTATTCC AGTTTTCATT 
GACAAGTGTA TTCGTTATAT TGAAGCCACA GGGTTAACAA CAGAGGGTAT CTACAGGGTA 
AGTGGGAATA AGGCGGAGAT CGAGGGCATG CAGAGGCAGT TTGAACAGGA CCACAATCTG 
GACTTTGTGG AGAAGGACTT CACTGTGAAC ACAGTTGCCG GGGCAATGAA AAGTTTCTTC 
TCTGAGCTGC CTGATCCTCT GGTTCCTTAC AGTTCACAGG AGGAGCTGGT GGAAGCTTTC 
AAAATTAATG ATCGAGAACA GAGACTTCAC ACAATGAAGG ACGTGTTAAG ACGATTTCCC 
AGGGAGAATT TTGATGTCTT CAAGTATGTA ATGAGCCATC TAAACAAGGT TGGTCAGTGG 
AACAGAGTCA ACTTAATGAC CAGTGAGAAT CTCTCCATCT GTTTCTGGCC AACACTGATG 
CGGCCAGACT TCACTTCTAT GGATGCCCTG ACCGCCACAC GCACCTACCA GACAATCATC 
GAGACGTTTA TCCATCAATG TGCTTTCTTC TTTTACAACC AGCCTCCTGC TGAATCGCCC 
ACTGGCCTAT TCGGCCAGCC TGGACCAATG GTGCCCTGTG GATTGCCCCC ATCCCCCACA 
GCTGCTCCTT CTTATTGCCC TTCGACCCCA CATTTTACCC CTCTTCTTCA GTCCCCGCCT 
CATTCTCCCC CCCAGAGCCC ACACTCCGCC CCTGCAGAAC CACAAACACT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgap35 ( XM_009301690.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009299965.1 Danio rerio Rho GTPase activating protein 35b (arhgap35), transcript variant X2, mRNA.

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CloneID	ODa44355
Clone ID Related Accession (Same CDS sequence)	XM_003198643.5 , XM_009301690.3 , XM_021476553.1 , XM_009301690.2 , XM_003198643.4 , XM_021476554.1
Accession Version	XM_009301690.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4554bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	rho GTPase-activating protein 35
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301690.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGG CTAAAAAGCA AGAATCCCGG CTGCCAATCT ACAACCTTGT TGTTGTTGGC 
CTGTCGGGAA CTGAGAAGGA GAAAGGGCAA TGTGGAGTTG GCAAATCTTG CCTTTGTAAT 
CGCTTTGTTC GCCCTAGTGC AGATGACTTC CATCTCGATC ACACCTCTGT GCTAAGTACT 
AGTGACTTTG GTGGCCGGGT TGTCAACAAT GACCATTTCC TGTTCTGGGG GGAGGTTGGG 
AGAGTTTTGG AGGATGGTGC AGACTGCAGA ATGCATGTGG TCGAGCAGAC AGAATTTATT 
GATGACCAGA CTTTCCAGCC CCATCGTAGC ACAGCCTTAC TGCCCTACAT CAAAAGAGCT 
GCCTCCACCA AATTGGCTTC TGCGGAAAAA CTCATGTACT TCTGCACTGA TCAGTTGGGC 
CTGGAGCAGG ACTTCGAACA GAAACAAATG CCTGAAGGCA AGTTGCAGGT CGATGGCTTT 
CTGCTTTGTG TTGATGTCAG TCGTGGTATG AACCGCAACT TTGATGATCA AATGAAATTT 
GTCACCAACT TGTACAATCA CTTAAGCAAG ACAAAAAAGC CTATTGTGCT GGTTCTCACC 
AAGTGTGATG AGGGTGTGGA GCGATACATC AAGGATTCTC ATACCTTTGC CATTACAAAA 
AAGAACCTGC AAGTTGTTGA GACTTCGGCT CGATCCAATA TCAATGTTGA CTTGGCTTTT 
CTTACCTTAA TTCAACTCAT TGATAAGGGA AAAGGTAAGC CTAAGATCAT ACCTTACTTT 
GAGGCATTGA AACACCAGAG TCAACTGATT GCCTCCGCAA AAGACCGCTA TGAATGGTTG 
GTGAACCACA TTGTGAAGAA TCACAATGAA ACTTGGCCAA ACGTCAGCAG ACGTATGCAG 
ACTTCACCCG AATATAAGGA ATATGTGTTT TTAGAAGGCA CAGGTAAAGC TAAGAAACTA 
TTTCAACAGC ACATTCACCG ACTTAAACAA GAGCATACTG AAAGACGTCG TAAAGCATAC 
TTGAGTGCTC TTCCACTCGC CTTAAGTAGC TTGGTGTCGG AGTTGGATGA AATTGAGCAT 
TTAAGCTGGT CTGGGGTGCA AAAAGTGCTT GAGTCTAAAA AAGACTTTGA TCATTGGTTT 
GTGGTACTGG ACGATGCACC ATGGGAAGAC ACACCACATC TTGACAATAT GGAGGATGAC 
CGCATCCCCT TTGATGTGCT AGACACCACG GCTGCTGAAA TCCTCTTTGA TGCACACCTT 
GAACACTTGC GCAATGAATG CAAGCGTGCC CAGATGCGTC ATGAGTTCAA AGGAAAATTA 
GCATCTTCAC CTTTCGTTAC TCCAGGCAAG CCCTGGGAGG AGGCTCGAAG CTTCATCATG 
AATGAAGACT TTTACCAGTG GCTTGAGGAG CCAGAATATT TGGACATTTA TAACCGTCAC 
CAGAAAGCAA TCATTGACAG GGCAAAAGAG GATTTTCAGG AGCTTCTGCT GGAGTACTCT 
GAACTCTTCT ACGAACTTGA GGTGGATGCC AAACCTAGTA AAGAGAAAAT GGGAGCAATT 
CAAGAAGTGT TAGGTGAAGA ACAACGCTTC AAGGCACTTC AGAAACTGCA AGCTGAAAGG 
GATGCTTTAG TACTGAAACA CATCCATTTT GTTTATCACC CTACGAAGGA AACATGCCCA 
AACTGCCCAC ACTGTGTTGA CAGCAAAATT GAGCAAATTC TAGCTTCATG CTTCCCTGCA 
CAATATCCTT CCTTTGGAAA GTCGCATATA ACTGATGCAA AGGTAGATAG GATCAATCTT 
GTTATACTGG GTAAAGATGG ACTTGCTAGA GAATTAGCCA ATGAAATTCG GGCACTGTGT 
ACTAATGATG ATCATTATGT GCTTGAAGGA AGAATGTATG AGCTTGTCCT TCGGCCAATT 
GAGGGCAATG TTAGACTTCC TGTTAATTCG TTTCATACAT CCACATTTAC TCCCCATGGA 
TGCCTGTGTT TGTACAATTC AAAAGAGTCC TTGTCCTATG TTGTAGAAAG CATTGAGAAG 
TTGCGAGAGT CCACACTTGG CAGAAGGGAC AACCATATGG CTCAGCTTCC GTTGTCCCTC 
TTATTGGTAA GCAAAAGAGG TGTTGGAGGA CTGAGTGATA TTGGTGGTGA GACTGCTCAA 
GCGTTAATCT CACAGGGTCA GCAAGTAGCC ATAAAACTGC AGTGTAACTA CCTGGAACCT 
TCCTCCCCAG GCACTGGTTA TGGGCGAAAC GTGAATGAAA AGCAGATCAA CCAGGTTTTG 
AAAGCTCTAC TTGAAACAAG GAGGACTTCT ACATTTCGTA GCTGTTCTCC ACCTGCACCT 
CCACTGCCTC CATGTATTCG AGACTTTCAG CAAGACCAGA GCCCAGAGGC TGATTTGCGT 
ATCGTCATGT GCCTAATGTG TGGAGACTCT TATGACATCG ATCAACTTCT TGCACCATTC 
CTGCTGCCTC AGCATTGCAA GCCAACTTCT CCCCAGACAA GTGGCACTTC AGTGCTGCTA 
GAACAAACAA TTGGTGGCCA CAAGCTTACG ATTGAGCTTT CTCTGCTGTC TTACCACTCT 
TCTTTTGCTA CTCGGAAGAG TCGACTGGTG CATGGCTACA TAGCTGTATA TTCTGCTAGC 
CGCAAGGCTT CTTTTGAAAC CCTGTGTGCC TTTTTGTGTG AGGTTCAGGA CATCATCCCG 
GTACAGATGA TTGCAGTGGG GGAAACACAG ATTGAGCTTC AGGACTCTGA GTCTGCTCGT 
GAATTGTTAG TCCAAGGTGA AGAGCTGGCA CATGAACTTG AAGGACGATT TGGCAGTATT 
GTCTGTGGTC ATGGCGGCGT TGTTGGAGGA CTACACAAAC TAGACCTTTT AGAACAATTT 
TTCACAGAAG TATTAGAAAA GAAAACCATA GTTGAAGCCA GCCATATGTA TGAAAGCATG 
GCAGAAGCCA GTAGCACAAA TGAGAATGTG TATTCGCCAC ACTGTGGCTC CCCAAGCCCT 
GTCACAATGC TGTTAGATTC TGAGGAAGAC ATGGAAGCTT CCCCGCCTTA CCATGATGGC 
ACACTTACCT CTCACGGTGG ATTCAAACTG CCTGACCTGG ACTCAGGTGA CACATTCTCT 
GTGATCTCTG ACCTTAGCAC CTTTGAGAAC AAGCTAAATA ATAAAGTTCC TCCACAAGTG 
AGACCCAAAC CTAGTGTCAC ATTTGACCTT AGAAAGCCAA GTTTTAACCC CTATGTGGAT 
GGAGTCAGCC ACCGCCGTTC CCTCACATCC AATGTGACGT GGCCTCTAGG AGGAGATTAT 
GATCCTTCAG ACTATGCTGA GCCAATGGAT GCTGTCTCTA AGCCACGCCC CAGCTATGAA 
GACAGCATAT ATTCTGTGCC TCATGACAGC ACACAAGGTA AAATTATTAC TATACGCAAT 
TCAAATCGAA TCCACTCCAA CGGAGGAGGA AATGGTTCAG ACAGTGAAGG GGATGGCAGC 
TCATTGGAGA GACGTCGCAA GTTTTCAGCA GCAAGGGTTA AGCCACGGCT TTACCGTGAT 
CGTTCCAAAC GTCTGGGGAA GTTCAGCAGT TTCCGCACTA GTTTCTCTAT TGGTAGCGAT 
GATGAGATTG GCACACTCTC AAGGTCAAAA GAGGATGATG TTTCACCACT GAAGACAGAT 
ATAACAAATG AAGAAGGAGA AGATCCCAAA AAGAGAAACA TCCTGAAAAG TCTCCGCAGA 
CCAGGCAAAA AAGCAAGACC AAAGCCTCGT CATTCCATCT CTAAGCCTTT GGAGAGTAAC 
TACTTTGGAG TGCCTCTGGT CAATGTTGTT TCCTTCGAAC GACCTATTCC AGTTTTCATT 
GACAAGTGTA TTCGTTATAT TGAAGCCACA GGGTTAACAA CAGAGGGTAT CTACAGGGTA 
AGTGGGAATA AGGCGGAGAT CGAGGGCATG CAGAGGCAGT TTGAACAGGA CCACAATCTG 
GACTTTGTGG AGAAGGACTT CACTGTGAAC ACAGTTGCCG GGGCAATGAA AAGTTTCTTC 
TCTGAGCTGC CTGATCCTCT GGTTCCTTAC AGTTCACAGG AGGAGCTGGT GGAAGCTTTC 
AAAATTAATG ATCGAGAACA GAGACTTCAC ACAATGAAGG ACGTGTTAAG ACGATTTCCC 
AGGGAGAATT TTGATGTCTT CAAGTATGTA ATGAGCCATC TAAACAAGGT TGGTCAGTGG 
AACAGAGTCA ACTTAATGAC CAGTGAGAAT CTCTCCATCT GTTTCTGGCC AACACTGATG 
CGGCCAGACT TCACTTCTAT GGATGCCCTG ACCGCCACAC GCACCTACCA GACAATCATC 
GAGACGTTTA TCCATCAATG TGCTTTCTTC TTTTACAACC AGCCTCCTGC TGAATCGCCC 
ACTGGCCTAT TCGGCCAGCC TGGACCAATG GTGCCCTGTG GATTGCCCCC ATCCCCCACA 
GCTGCTCCTT CTTATTGCCC TTCGACCCCA CATTTTACCC CTCTTCTTCA GTCCCCGCCT 
CATTCTCCCC CCCAGAGCCC ACACTCCGCC CCTGCAGAAC CACAAACACT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009299965.1
CDS	1046..5599
Translation	MMMAKKQESRLPIYNLVVVGLSGTEKEKGQCGVGKSCLCNRFVRPSADDFHLDHTSVLSTSDFGGRVVNNDHFLFWGEVGRVLEDGADCRMHVVEQTEFIDDQTFQPHRSTALLPYIKRAASTKLASAEKLMYFCTDQLGLEQDFEQKQMPEGKLQVDGFLLCVDVSRGMNRNFDDQMKFVTNLYNHLSKTKKPIVLVLTKCDEGVERYIKDSHTFAITKKNLQVVETSARSNINVDLAFLTLIQLIDKGKGKPKIIPYFEALKHQSQLIASAKDRYEWLVNHIVKNHNETWPNVSRRMQTSPEYKEYVFLEGTGKAKKLFQQHIHRLKQEHTERRRKAYLSALPLALSSLVSELDEIEHLSWSGVQKVLESKKDFDHWFVVLDDAPWEDTPHLDNMEDDRIPFDVLDTTAAEILFDAHLEHLRNECKRAQMRHEFKGKLASSPFVTPGKPWEEARSFIMNEDFYQWLEEPEYLDIYNRHQKAIIDRAKEDFQELLLEYSELFYELEVDAKPSKEKMGAIQEVLGEEQRFKALQKLQAERDALVLKHIHFVYHPTKETCPNCPHCVDSKIEQILASCFPAQYPSFGKSHITDAKVDRINLVILGKDGLARELANEIRALCTNDDHYVLEGRMYELVLRPIEGNVRLPVNSFHTSTFTPHGCLCLYNSKESLSYVVESIEKLRESTLGRRDNHMAQLPLSLLLVSKRGVGGLSDIGGETAQALISQGQQVAIKLQCNYLEPSSPGTGYGRNVNEKQINQVLKALLETRRTSTFRSCSPPAPPLPPCIRDFQQDQSPEADLRIVMCLMCGDSYDIDQLLAPFLLPQHCKPTSPQTSGTSVLLEQTIGGHKLTIELSLLSYHSSFATRKSRLVHGYIAVYSASRKASFETLCAFLCEVQDIIPVQMIAVGETQIELQDSESARELLVQGEELAHELEGRFGSIVCGHGGVVGGLHKLDLLEQFFTEVLEKKTIVEASHMYESMAEASSTNENVYSPHCGSPSPVTMLLDSEEDMEASPPYHDGTLTSHGGFKLPDLDSGDTFSVISDLSTFENKLNNKVPPQVRPKPSVTFDLRKPSFNPYVDGVSHRRSLTSNVTWPLGGDYDPSDYAEPMDAVSKPRPSYEDSIYSVPHDSTQGKIITIRNSNRIHSNGGGNGSDSEGDGSSLERRRKFSAARVKPRLYRDRSKRLGKFSSFRTSFSIGSDDEIGTLSRSKEDDVSPLKTDITNEEGEDPKKRNILKSLRRPGKKARPKPRHSISKPLESNYFGVPLVNVVSFERPIPVFIDKCIRYIEATGLTTEGIYRVSGNKAEIEGMQRQFEQDHNLDFVEKDFTVNTVAGAMKSFFSELPDPLVPYSSQEELVEAFKINDREQRLHTMKDVLRRFPRENFDVFKYVMSHLNKVGQWNRVNLMTSENLSICFWPTLMRPDFTSMDALTATRTYQTIIETFIHQCAFFFYNQPPAESPTGLFGQPGPMVPCGLPPSPTAAPSYCPSTPHFTPLLQSPPHSPPQSPHSAPAEPQTL

Target ORF information:

RefSeq Version	XM_009301690.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Rho GTPase activating protein 35b (arhgap35), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301690.2

ORF Insert Sequence:

ATGATGATGG CTAAAAAGCA AGAATCCCGG CTGCCAATCT ACAACCTTGT TGTTGTTGGC 
CTGTCGGGAA CTGAGAAGGA GAAAGGGCAA TGTGGAGTTG GCAAATCTTG CCTTTGTAAT 
CGCTTTGTTC GCCCTAGTGC AGATGACTTC CATCTCGATC ACACCTCTGT GCTAAGTACT 
AGTGACTTTG GTGGCCGGGT TGTCAACAAT GACCATTTCC TGTTCTGGGG GGAGGTTGGG 
AGAGTTTTGG AGGATGGTGC AGACTGCAGA ATGCATGTGG TCGAGCAGAC AGAATTTATT 
GATGACCAGA CTTTCCAGCC CCATCGTAGC ACAGCCTTAC TGCCCTACAT CAAAAGAGCT 
GCCTCCACCA AATTGGCTTC TGCGGAAAAA CTCATGTACT TCTGCACTGA TCAGTTGGGC 
CTGGAGCAGG ACTTCGAACA GAAACAAATG CCTGAAGGCA AGTTGCAGGT CGATGGCTTT 
CTGCTTTGTG TTGATGTCAG TCGTGGTATG AACCGCAACT TTGATGATCA AATGAAATTT 
GTCACCAACT TGTACAATCA CTTAAGCAAG ACAAAAAAGC CTATTGTGCT GGTTCTCACC 
AAGTGTGATG AGGGTGTGGA GCGATACATC AAGGATTCTC ATACCTTTGC CATTACAAAA 
AAGAACCTGC AAGTTGTTGA GACTTCGGCT CGATCCAATA TCAATGTTGA CTTGGCTTTT 
CTTACCTTAA TTCAACTCAT TGATAAGGGA AAAGGTAAGC CTAAGATCAT ACCTTACTTT 
GAGGCATTGA AACACCAGAG TCAACTGATT GCCTCCGCAA AAGACCGCTA TGAATGGTTG 
GTGAACCACA TTGTGAAGAA TCACAATGAA ACTTGGCCAA ACGTCAGCAG ACGTATGCAG 
ACTTCACCCG AATATAAGGA ATATGTGTTT TTAGAAGGCA CAGGTAAAGC TAAGAAACTA 
TTTCAACAGC ACATTCACCG ACTTAAACAA GAGCATACTG AAAGACGTCG TAAAGCATAC 
TTGAGTGCTC TTCCACTCGC CTTAAGTAGC TTGGTGTCGG AGTTGGATGA AATTGAGCAT 
TTAAGCTGGT CTGGGGTGCA AAAAGTGCTT GAGTCTAAAA AAGACTTTGA TCATTGGTTT 
GTGGTACTGG ACGATGCACC ATGGGAAGAC ACACCACATC TTGACAATAT GGAGGATGAC 
CGCATCCCCT TTGATGTGCT AGACACCACG GCTGCTGAAA TCCTCTTTGA TGCACACCTT 
GAACACTTGC GCAATGAATG CAAGCGTGCC CAGATGCGTC ATGAGTTCAA AGGAAAATTA 
GCATCTTCAC CTTTCGTTAC TCCAGGCAAG CCCTGGGAGG AGGCTCGAAG CTTCATCATG 
AATGAAGACT TTTACCAGTG GCTTGAGGAG CCAGAATATT TGGACATTTA TAACCGTCAC 
CAGAAAGCAA TCATTGACAG GGCAAAAGAG GATTTTCAGG AGCTTCTGCT GGAGTACTCT 
GAACTCTTCT ACGAACTTGA GGTGGATGCC AAACCTAGTA AAGAGAAAAT GGGAGCAATT 
CAAGAAGTGT TAGGTGAAGA ACAACGCTTC AAGGCACTTC AGAAACTGCA AGCTGAAAGG 
GATGCTTTAG TACTGAAACA CATCCATTTT GTTTATCACC CTACGAAGGA AACATGCCCA 
AACTGCCCAC ACTGTGTTGA CAGCAAAATT GAGCAAATTC TAGCTTCATG CTTCCCTGCA 
CAATATCCTT CCTTTGGAAA GTCGCATATA ACTGATGCAA AGGTAGATAG GATCAATCTT 
GTTATACTGG GTAAAGATGG ACTTGCTAGA GAATTAGCCA ATGAAATTCG GGCACTGTGT 
ACTAATGATG ATCATTATGT GCTTGAAGGA AGAATGTATG AGCTTGTCCT TCGGCCAATT 
GAGGGCAATG TTAGACTTCC TGTTAATTCG TTTCATACAT CCACATTTAC TCCCCATGGA 
TGCCTGTGTT TGTACAATTC AAAAGAGTCC TTGTCCTATG TTGTAGAAAG CATTGAGAAG 
TTGCGAGAGT CCACACTTGG CAGAAGGGAC AACCATATGG CTCAGCTTCC GTTGTCCCTC 
TTATTGGTAA GCAAAAGAGG TGTTGGAGGA CTGAGTGATA TTGGTGGTGA GACTGCTCAA 
GCGTTAATCT CACAGGGTCA GCAAGTAGCC ATAAAACTGC AGTGTAACTA CCTGGAACCT 
TCCTCCCCAG GCACTGGTTA TGGGCGAAAC GTGAATGAAA AGCAGATCAA CCAGGTTTTG 
AAAGCTCTAC TTGAAACAAG GAGGACTTCT ACATTTCGTA GCTGTTCTCC ACCTGCACCT 
CCACTGCCTC CATGTATTCG AGACTTTCAG CAAGACCAGA GCCCAGAGGC TGATTTGCGT 
ATCGTCATGT GCCTAATGTG TGGAGACTCT TATGACATCG ATCAACTTCT TGCACCATTC 
CTGCTGCCTC AGCATTGCAA GCCAACTTCT CCCCAGACAA GTGGCACTTC AGTGCTGCTA 
GAACAAACAA TTGGTGGCCA CAAGCTTACG ATTGAGCTTT CTCTGCTGTC TTACCACTCT 
TCTTTTGCTA CTCGGAAGAG TCGACTGGTG CATGGCTACA TAGCTGTATA TTCTGCTAGC 
CGCAAGGCTT CTTTTGAAAC CCTGTGTGCC TTTTTGTGTG AGGTTCAGGA CATCATCCCG 
GTACAGATGA TTGCAGTGGG GGAAACACAG ATTGAGCTTC AGGACTCTGA GTCTGCTCGT 
GAATTGTTAG TCCAAGGTGA AGAGCTGGCA CATGAACTTG AAGGACGATT TGGCAGTATT 
GTCTGTGGTC ATGGCGGCGT TGTTGGAGGA CTACACAAAC TAGACCTTTT AGAACAATTT 
TTCACAGAAG TATTAGAAAA GAAAACCATA GTTGAAGCCA GCCATATGTA TGAAAGCATG 
GCAGAAGCCA GTAGCACAAA TGAGAATGTG TATTCGCCAC ACTGTGGCTC CCCAAGCCCT 
GTCACAATGC TGTTAGATTC TGAGGAAGAC ATGGAAGCTT CCCCGCCTTA CCATGATGGC 
ACACTTACCT CTCACGGTGG ATTCAAACTG CCTGACCTGG ACTCAGGTGA CACATTCTCT 
GTGATCTCTG ACCTTAGCAC CTTTGAGAAC AAGCTAAATA ATAAAGTTCC TCCACAAGTG 
AGACCCAAAC CTAGTGTCAC ATTTGACCTT AGAAAGCCAA GTTTTAACCC CTATGTGGAT 
GGAGTCAGCC ACCGCCGTTC CCTCACATCC AATGTGACGT GGCCTCTAGG AGGAGATTAT 
GATCCTTCAG ACTATGCTGA GCCAATGGAT GCTGTCTCTA AGCCACGCCC CAGCTATGAA 
GACAGCATAT ATTCTGTGCC TCATGACAGC ACACAAGGTA AAATTATTAC TATACGCAAT 
TCAAATCGAA TCCACTCCAA CGGAGGAGGA AATGGTTCAG ACAGTGAAGG GGATGGCAGC 
TCATTGGAGA GACGTCGCAA GTTTTCAGCA GCAAGGGTTA AGCCACGGCT TTACCGTGAT 
CGTTCCAAAC GTCTGGGGAA GTTCAGCAGT TTCCGCACTA GTTTCTCTAT TGGTAGCGAT 
GATGAGATTG GCACACTCTC AAGGTCAAAA GAGGATGATG TTTCACCACT GAAGACAGAT 
ATAACAAATG AAGAAGGAGA AGATCCCAAA AAGAGAAACA TCCTGAAAAG TCTCCGCAGA 
CCAGGCAAAA AAGCAAGACC AAAGCCTCGT CATTCCATCT CTAAGCCTTT GGAGAGTAAC 
TACTTTGGAG TGCCTCTGGT CAATGTTGTT TCCTTCGAAC GACCTATTCC AGTTTTCATT 
GACAAGTGTA TTCGTTATAT TGAAGCCACA GGGTTAACAA CAGAGGGTAT CTACAGGGTA 
AGTGGGAATA AGGCGGAGAT CGAGGGCATG CAGAGGCAGT TTGAACAGGA CCACAATCTG 
GACTTTGTGG AGAAGGACTT CACTGTGAAC ACAGTTGCCG GGGCAATGAA AAGTTTCTTC 
TCTGAGCTGC CTGATCCTCT GGTTCCTTAC AGTTCACAGG AGGAGCTGGT GGAAGCTTTC 
AAAATTAATG ATCGAGAACA GAGACTTCAC ACAATGAAGG ACGTGTTAAG ACGATTTCCC 
AGGGAGAATT TTGATGTCTT CAAGTATGTA ATGAGCCATC TAAACAAGGT TGGTCAGTGG 
AACAGAGTCA ACTTAATGAC CAGTGAGAAT CTCTCCATCT GTTTCTGGCC AACACTGATG 
CGGCCAGACT TCACTTCTAT GGATGCCCTG ACCGCCACAC GCACCTACCA GACAATCATC 
GAGACGTTTA TCCATCAATG TGCTTTCTTC TTTTACAACC AGCCTCCTGC TGAATCGCCC 
ACTGGCCTAT TCGGCCAGCC TGGACCAATG GTGCCCTGTG GATTGCCCCC ATCCCCCACA 
GCTGCTCCTT CTTATTGCCC TTCGACCCCA CATTTTACCC CTCTTCTTCA GTCCCCGCCT 
CATTCTCCCC CCCAGAGCCC ACACTCCGCC CCTGCAGAAC CACAAACACT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgap35 ( XM_003198643.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_003198691.2 Danio rerio Rho GTPase activating protein 35b (arhgap35), transcript variant X1, mRNA.

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CloneID	ODa44355
Clone ID Related Accession (Same CDS sequence)	XM_003198643.5 , XM_009301690.3 , XM_021476553.1 , XM_009301690.2 , XM_003198643.4 , XM_021476554.1
Accession Version	XM_003198643.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4554bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	rho GTPase-activating protein 35
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_003198643.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGG CTAAAAAGCA AGAATCCCGG CTGCCAATCT ACAACCTTGT TGTTGTTGGC 
CTGTCGGGAA CTGAGAAGGA GAAAGGGCAA TGTGGAGTTG GCAAATCTTG CCTTTGTAAT 
CGCTTTGTTC GCCCTAGTGC AGATGACTTC CATCTCGATC ACACCTCTGT GCTAAGTACT 
AGTGACTTTG GTGGCCGGGT TGTCAACAAT GACCATTTCC TGTTCTGGGG GGAGGTTGGG 
AGAGTTTTGG AGGATGGTGC AGACTGCAGA ATGCATGTGG TCGAGCAGAC AGAATTTATT 
GATGACCAGA CTTTCCAGCC CCATCGTAGC ACAGCCTTAC TGCCCTACAT CAAAAGAGCT 
GCCTCCACCA AATTGGCTTC TGCGGAAAAA CTCATGTACT TCTGCACTGA TCAGTTGGGC 
CTGGAGCAGG ACTTCGAACA GAAACAAATG CCTGAAGGCA AGTTGCAGGT CGATGGCTTT 
CTGCTTTGTG TTGATGTCAG TCGTGGTATG AACCGCAACT TTGATGATCA AATGAAATTT 
GTCACCAACT TGTACAATCA CTTAAGCAAG ACAAAAAAGC CTATTGTGCT GGTTCTCACC 
AAGTGTGATG AGGGTGTGGA GCGATACATC AAGGATTCTC ATACCTTTGC CATTACAAAA 
AAGAACCTGC AAGTTGTTGA GACTTCGGCT CGATCCAATA TCAATGTTGA CTTGGCTTTT 
CTTACCTTAA TTCAACTCAT TGATAAGGGA AAAGGTAAGC CTAAGATCAT ACCTTACTTT 
GAGGCATTGA AACACCAGAG TCAACTGATT GCCTCCGCAA AAGACCGCTA TGAATGGTTG 
GTGAACCACA TTGTGAAGAA TCACAATGAA ACTTGGCCAA ACGTCAGCAG ACGTATGCAG 
ACTTCACCCG AATATAAGGA ATATGTGTTT TTAGAAGGCA CAGGTAAAGC TAAGAAACTA 
TTTCAACAGC ACATTCACCG ACTTAAACAA GAGCATACTG AAAGACGTCG TAAAGCATAC 
TTGAGTGCTC TTCCACTCGC CTTAAGTAGC TTGGTGTCGG AGTTGGATGA AATTGAGCAT 
TTAAGCTGGT CTGGGGTGCA AAAAGTGCTT GAGTCTAAAA AAGACTTTGA TCATTGGTTT 
GTGGTACTGG ACGATGCACC ATGGGAAGAC ACACCACATC TTGACAATAT GGAGGATGAC 
CGCATCCCCT TTGATGTGCT AGACACCACG GCTGCTGAAA TCCTCTTTGA TGCACACCTT 
GAACACTTGC GCAATGAATG CAAGCGTGCC CAGATGCGTC ATGAGTTCAA AGGAAAATTA 
GCATCTTCAC CTTTCGTTAC TCCAGGCAAG CCCTGGGAGG AGGCTCGAAG CTTCATCATG 
AATGAAGACT TTTACCAGTG GCTTGAGGAG CCAGAATATT TGGACATTTA TAACCGTCAC 
CAGAAAGCAA TCATTGACAG GGCAAAAGAG GATTTTCAGG AGCTTCTGCT GGAGTACTCT 
GAACTCTTCT ACGAACTTGA GGTGGATGCC AAACCTAGTA AAGAGAAAAT GGGAGCAATT 
CAAGAAGTGT TAGGTGAAGA ACAACGCTTC AAGGCACTTC AGAAACTGCA AGCTGAAAGG 
GATGCTTTAG TACTGAAACA CATCCATTTT GTTTATCACC CTACGAAGGA AACATGCCCA 
AACTGCCCAC ACTGTGTTGA CAGCAAAATT GAGCAAATTC TAGCTTCATG CTTCCCTGCA 
CAATATCCTT CCTTTGGAAA GTCGCATATA ACTGATGCAA AGGTAGATAG GATCAATCTT 
GTTATACTGG GTAAAGATGG ACTTGCTAGA GAATTAGCCA ATGAAATTCG GGCACTGTGT 
ACTAATGATG ATCATTATGT GCTTGAAGGA AGAATGTATG AGCTTGTCCT TCGGCCAATT 
GAGGGCAATG TTAGACTTCC TGTTAATTCG TTTCATACAT CCACATTTAC TCCCCATGGA 
TGCCTGTGTT TGTACAATTC AAAAGAGTCC TTGTCCTATG TTGTAGAAAG CATTGAGAAG 
TTGCGAGAGT CCACACTTGG CAGAAGGGAC AACCATATGG CTCAGCTTCC GTTGTCCCTC 
TTATTGGTAA GCAAAAGAGG TGTTGGAGGA CTGAGTGATA TTGGTGGTGA GACTGCTCAA 
GCGTTAATCT CACAGGGTCA GCAAGTAGCC ATAAAACTGC AGTGTAACTA CCTGGAACCT 
TCCTCCCCAG GCACTGGTTA TGGGCGAAAC GTGAATGAAA AGCAGATCAA CCAGGTTTTG 
AAAGCTCTAC TTGAAACAAG GAGGACTTCT ACATTTCGTA GCTGTTCTCC ACCTGCACCT 
CCACTGCCTC CATGTATTCG AGACTTTCAG CAAGACCAGA GCCCAGAGGC TGATTTGCGT 
ATCGTCATGT GCCTAATGTG TGGAGACTCT TATGACATCG ATCAACTTCT TGCACCATTC 
CTGCTGCCTC AGCATTGCAA GCCAACTTCT CCCCAGACAA GTGGCACTTC AGTGCTGCTA 
GAACAAACAA TTGGTGGCCA CAAGCTTACG ATTGAGCTTT CTCTGCTGTC TTACCACTCT 
TCTTTTGCTA CTCGGAAGAG TCGACTGGTG CATGGCTACA TAGCTGTATA TTCTGCTAGC 
CGCAAGGCTT CTTTTGAAAC CCTGTGTGCC TTTTTGTGTG AGGTTCAGGA CATCATCCCG 
GTACAGATGA TTGCAGTGGG GGAAACACAG ATTGAGCTTC AGGACTCTGA GTCTGCTCGT 
GAATTGTTAG TCCAAGGTGA AGAGCTGGCA CATGAACTTG AAGGACGATT TGGCAGTATT 
GTCTGTGGTC ATGGCGGCGT TGTTGGAGGA CTACACAAAC TAGACCTTTT AGAACAATTT 
TTCACAGAAG TATTAGAAAA GAAAACCATA GTTGAAGCCA GCCATATGTA TGAAAGCATG 
GCAGAAGCCA GTAGCACAAA TGAGAATGTG TATTCGCCAC ACTGTGGCTC CCCAAGCCCT 
GTCACAATGC TGTTAGATTC TGAGGAAGAC ATGGAAGCTT CCCCGCCTTA CCATGATGGC 
ACACTTACCT CTCACGGTGG ATTCAAACTG CCTGACCTGG ACTCAGGTGA CACATTCTCT 
GTGATCTCTG ACCTTAGCAC CTTTGAGAAC AAGCTAAATA ATAAAGTTCC TCCACAAGTG 
AGACCCAAAC CTAGTGTCAC ATTTGACCTT AGAAAGCCAA GTTTTAACCC CTATGTGGAT 
GGAGTCAGCC ACCGCCGTTC CCTCACATCC AATGTGACGT GGCCTCTAGG AGGAGATTAT 
GATCCTTCAG ACTATGCTGA GCCAATGGAT GCTGTCTCTA AGCCACGCCC CAGCTATGAA 
GACAGCATAT ATTCTGTGCC TCATGACAGC ACACAAGGTA AAATTATTAC TATACGCAAT 
TCAAATCGAA TCCACTCCAA CGGAGGAGGA AATGGTTCAG ACAGTGAAGG GGATGGCAGC 
TCATTGGAGA GACGTCGCAA GTTTTCAGCA GCAAGGGTTA AGCCACGGCT TTACCGTGAT 
CGTTCCAAAC GTCTGGGGAA GTTCAGCAGT TTCCGCACTA GTTTCTCTAT TGGTAGCGAT 
GATGAGATTG GCACACTCTC AAGGTCAAAA GAGGATGATG TTTCACCACT GAAGACAGAT 
ATAACAAATG AAGAAGGAGA AGATCCCAAA AAGAGAAACA TCCTGAAAAG TCTCCGCAGA 
CCAGGCAAAA AAGCAAGACC AAAGCCTCGT CATTCCATCT CTAAGCCTTT GGAGAGTAAC 
TACTTTGGAG TGCCTCTGGT CAATGTTGTT TCCTTCGAAC GACCTATTCC AGTTTTCATT 
GACAAGTGTA TTCGTTATAT TGAAGCCACA GGGTTAACAA CAGAGGGTAT CTACAGGGTA 
AGTGGGAATA AGGCGGAGAT CGAGGGCATG CAGAGGCAGT TTGAACAGGA CCACAATCTG 
GACTTTGTGG AGAAGGACTT CACTGTGAAC ACAGTTGCCG GGGCAATGAA AAGTTTCTTC 
TCTGAGCTGC CTGATCCTCT GGTTCCTTAC AGTTCACAGG AGGAGCTGGT GGAAGCTTTC 
AAAATTAATG ATCGAGAACA GAGACTTCAC ACAATGAAGG ACGTGTTAAG ACGATTTCCC 
AGGGAGAATT TTGATGTCTT CAAGTATGTA ATGAGCCATC TAAACAAGGT TGGTCAGTGG 
AACAGAGTCA ACTTAATGAC CAGTGAGAAT CTCTCCATCT GTTTCTGGCC AACACTGATG 
CGGCCAGACT TCACTTCTAT GGATGCCCTG ACCGCCACAC GCACCTACCA GACAATCATC 
GAGACGTTTA TCCATCAATG TGCTTTCTTC TTTTACAACC AGCCTCCTGC TGAATCGCCC 
ACTGGCCTAT TCGGCCAGCC TGGACCAATG GTGCCCTGTG GATTGCCCCC ATCCCCCACA 
GCTGCTCCTT CTTATTGCCC TTCGACCCCA CATTTTACCC CTCTTCTTCA GTCCCCGCCT 
CATTCTCCCC CCCAGAGCCC ACACTCCGCC CCTGCAGAAC CACAAACACT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003198691.2
CDS	1042..5595
Translation	MMMAKKQESRLPIYNLVVVGLSGTEKEKGQCGVGKSCLCNRFVRPSADDFHLDHTSVLSTSDFGGRVVNNDHFLFWGEVGRVLEDGADCRMHVVEQTEFIDDQTFQPHRSTALLPYIKRAASTKLASAEKLMYFCTDQLGLEQDFEQKQMPEGKLQVDGFLLCVDVSRGMNRNFDDQMKFVTNLYNHLSKTKKPIVLVLTKCDEGVERYIKDSHTFAITKKNLQVVETSARSNINVDLAFLTLIQLIDKGKGKPKIIPYFEALKHQSQLIASAKDRYEWLVNHIVKNHNETWPNVSRRMQTSPEYKEYVFLEGTGKAKKLFQQHIHRLKQEHTERRRKAYLSALPLALSSLVSELDEIEHLSWSGVQKVLESKKDFDHWFVVLDDAPWEDTPHLDNMEDDRIPFDVLDTTAAEILFDAHLEHLRNECKRAQMRHEFKGKLASSPFVTPGKPWEEARSFIMNEDFYQWLEEPEYLDIYNRHQKAIIDRAKEDFQELLLEYSELFYELEVDAKPSKEKMGAIQEVLGEEQRFKALQKLQAERDALVLKHIHFVYHPTKETCPNCPHCVDSKIEQILASCFPAQYPSFGKSHITDAKVDRINLVILGKDGLARELANEIRALCTNDDHYVLEGRMYELVLRPIEGNVRLPVNSFHTSTFTPHGCLCLYNSKESLSYVVESIEKLRESTLGRRDNHMAQLPLSLLLVSKRGVGGLSDIGGETAQALISQGQQVAIKLQCNYLEPSSPGTGYGRNVNEKQINQVLKALLETRRTSTFRSCSPPAPPLPPCIRDFQQDQSPEADLRIVMCLMCGDSYDIDQLLAPFLLPQHCKPTSPQTSGTSVLLEQTIGGHKLTIELSLLSYHSSFATRKSRLVHGYIAVYSASRKASFETLCAFLCEVQDIIPVQMIAVGETQIELQDSESARELLVQGEELAHELEGRFGSIVCGHGGVVGGLHKLDLLEQFFTEVLEKKTIVEASHMYESMAEASSTNENVYSPHCGSPSPVTMLLDSEEDMEASPPYHDGTLTSHGGFKLPDLDSGDTFSVISDLSTFENKLNNKVPPQVRPKPSVTFDLRKPSFNPYVDGVSHRRSLTSNVTWPLGGDYDPSDYAEPMDAVSKPRPSYEDSIYSVPHDSTQGKIITIRNSNRIHSNGGGNGSDSEGDGSSLERRRKFSAARVKPRLYRDRSKRLGKFSSFRTSFSIGSDDEIGTLSRSKEDDVSPLKTDITNEEGEDPKKRNILKSLRRPGKKARPKPRHSISKPLESNYFGVPLVNVVSFERPIPVFIDKCIRYIEATGLTTEGIYRVSGNKAEIEGMQRQFEQDHNLDFVEKDFTVNTVAGAMKSFFSELPDPLVPYSSQEELVEAFKINDREQRLHTMKDVLRRFPRENFDVFKYVMSHLNKVGQWNRVNLMTSENLSICFWPTLMRPDFTSMDALTATRTYQTIIETFIHQCAFFFYNQPPAESPTGLFGQPGPMVPCGLPPSPTAAPSYCPSTPHFTPLLQSPPHSPPQSPHSAPAEPQTL

Target ORF information:

RefSeq Version	XM_003198643.4
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Rho GTPase activating protein 35b (arhgap35), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003198643.4

ORF Insert Sequence:

ATGATGATGG CTAAAAAGCA AGAATCCCGG CTGCCAATCT ACAACCTTGT TGTTGTTGGC 
CTGTCGGGAA CTGAGAAGGA GAAAGGGCAA TGTGGAGTTG GCAAATCTTG CCTTTGTAAT 
CGCTTTGTTC GCCCTAGTGC AGATGACTTC CATCTCGATC ACACCTCTGT GCTAAGTACT 
AGTGACTTTG GTGGCCGGGT TGTCAACAAT GACCATTTCC TGTTCTGGGG GGAGGTTGGG 
AGAGTTTTGG AGGATGGTGC AGACTGCAGA ATGCATGTGG TCGAGCAGAC AGAATTTATT 
GATGACCAGA CTTTCCAGCC CCATCGTAGC ACAGCCTTAC TGCCCTACAT CAAAAGAGCT 
GCCTCCACCA AATTGGCTTC TGCGGAAAAA CTCATGTACT TCTGCACTGA TCAGTTGGGC 
CTGGAGCAGG ACTTCGAACA GAAACAAATG CCTGAAGGCA AGTTGCAGGT CGATGGCTTT 
CTGCTTTGTG TTGATGTCAG TCGTGGTATG AACCGCAACT TTGATGATCA AATGAAATTT 
GTCACCAACT TGTACAATCA CTTAAGCAAG ACAAAAAAGC CTATTGTGCT GGTTCTCACC 
AAGTGTGATG AGGGTGTGGA GCGATACATC AAGGATTCTC ATACCTTTGC CATTACAAAA 
AAGAACCTGC AAGTTGTTGA GACTTCGGCT CGATCCAATA TCAATGTTGA CTTGGCTTTT 
CTTACCTTAA TTCAACTCAT TGATAAGGGA AAAGGTAAGC CTAAGATCAT ACCTTACTTT 
GAGGCATTGA AACACCAGAG TCAACTGATT GCCTCCGCAA AAGACCGCTA TGAATGGTTG 
GTGAACCACA TTGTGAAGAA TCACAATGAA ACTTGGCCAA ACGTCAGCAG ACGTATGCAG 
ACTTCACCCG AATATAAGGA ATATGTGTTT TTAGAAGGCA CAGGTAAAGC TAAGAAACTA 
TTTCAACAGC ACATTCACCG ACTTAAACAA GAGCATACTG AAAGACGTCG TAAAGCATAC 
TTGAGTGCTC TTCCACTCGC CTTAAGTAGC TTGGTGTCGG AGTTGGATGA AATTGAGCAT 
TTAAGCTGGT CTGGGGTGCA AAAAGTGCTT GAGTCTAAAA AAGACTTTGA TCATTGGTTT 
GTGGTACTGG ACGATGCACC ATGGGAAGAC ACACCACATC TTGACAATAT GGAGGATGAC 
CGCATCCCCT TTGATGTGCT AGACACCACG GCTGCTGAAA TCCTCTTTGA TGCACACCTT 
GAACACTTGC GCAATGAATG CAAGCGTGCC CAGATGCGTC ATGAGTTCAA AGGAAAATTA 
GCATCTTCAC CTTTCGTTAC TCCAGGCAAG CCCTGGGAGG AGGCTCGAAG CTTCATCATG 
AATGAAGACT TTTACCAGTG GCTTGAGGAG CCAGAATATT TGGACATTTA TAACCGTCAC 
CAGAAAGCAA TCATTGACAG GGCAAAAGAG GATTTTCAGG AGCTTCTGCT GGAGTACTCT 
GAACTCTTCT ACGAACTTGA GGTGGATGCC AAACCTAGTA AAGAGAAAAT GGGAGCAATT 
CAAGAAGTGT TAGGTGAAGA ACAACGCTTC AAGGCACTTC AGAAACTGCA AGCTGAAAGG 
GATGCTTTAG TACTGAAACA CATCCATTTT GTTTATCACC CTACGAAGGA AACATGCCCA 
AACTGCCCAC ACTGTGTTGA CAGCAAAATT GAGCAAATTC TAGCTTCATG CTTCCCTGCA 
CAATATCCTT CCTTTGGAAA GTCGCATATA ACTGATGCAA AGGTAGATAG GATCAATCTT 
GTTATACTGG GTAAAGATGG ACTTGCTAGA GAATTAGCCA ATGAAATTCG GGCACTGTGT 
ACTAATGATG ATCATTATGT GCTTGAAGGA AGAATGTATG AGCTTGTCCT TCGGCCAATT 
GAGGGCAATG TTAGACTTCC TGTTAATTCG TTTCATACAT CCACATTTAC TCCCCATGGA 
TGCCTGTGTT TGTACAATTC AAAAGAGTCC TTGTCCTATG TTGTAGAAAG CATTGAGAAG 
TTGCGAGAGT CCACACTTGG CAGAAGGGAC AACCATATGG CTCAGCTTCC GTTGTCCCTC 
TTATTGGTAA GCAAAAGAGG TGTTGGAGGA CTGAGTGATA TTGGTGGTGA GACTGCTCAA 
GCGTTAATCT CACAGGGTCA GCAAGTAGCC ATAAAACTGC AGTGTAACTA CCTGGAACCT 
TCCTCCCCAG GCACTGGTTA TGGGCGAAAC GTGAATGAAA AGCAGATCAA CCAGGTTTTG 
AAAGCTCTAC TTGAAACAAG GAGGACTTCT ACATTTCGTA GCTGTTCTCC ACCTGCACCT 
CCACTGCCTC CATGTATTCG AGACTTTCAG CAAGACCAGA GCCCAGAGGC TGATTTGCGT 
ATCGTCATGT GCCTAATGTG TGGAGACTCT TATGACATCG ATCAACTTCT TGCACCATTC 
CTGCTGCCTC AGCATTGCAA GCCAACTTCT CCCCAGACAA GTGGCACTTC AGTGCTGCTA 
GAACAAACAA TTGGTGGCCA CAAGCTTACG ATTGAGCTTT CTCTGCTGTC TTACCACTCT 
TCTTTTGCTA CTCGGAAGAG TCGACTGGTG CATGGCTACA TAGCTGTATA TTCTGCTAGC 
CGCAAGGCTT CTTTTGAAAC CCTGTGTGCC TTTTTGTGTG AGGTTCAGGA CATCATCCCG 
GTACAGATGA TTGCAGTGGG GGAAACACAG ATTGAGCTTC AGGACTCTGA GTCTGCTCGT 
GAATTGTTAG TCCAAGGTGA AGAGCTGGCA CATGAACTTG AAGGACGATT TGGCAGTATT 
GTCTGTGGTC ATGGCGGCGT TGTTGGAGGA CTACACAAAC TAGACCTTTT AGAACAATTT 
TTCACAGAAG TATTAGAAAA GAAAACCATA GTTGAAGCCA GCCATATGTA TGAAAGCATG 
GCAGAAGCCA GTAGCACAAA TGAGAATGTG TATTCGCCAC ACTGTGGCTC CCCAAGCCCT 
GTCACAATGC TGTTAGATTC TGAGGAAGAC ATGGAAGCTT CCCCGCCTTA CCATGATGGC 
ACACTTACCT CTCACGGTGG ATTCAAACTG CCTGACCTGG ACTCAGGTGA CACATTCTCT 
GTGATCTCTG ACCTTAGCAC CTTTGAGAAC AAGCTAAATA ATAAAGTTCC TCCACAAGTG 
AGACCCAAAC CTAGTGTCAC ATTTGACCTT AGAAAGCCAA GTTTTAACCC CTATGTGGAT 
GGAGTCAGCC ACCGCCGTTC CCTCACATCC AATGTGACGT GGCCTCTAGG AGGAGATTAT 
GATCCTTCAG ACTATGCTGA GCCAATGGAT GCTGTCTCTA AGCCACGCCC CAGCTATGAA 
GACAGCATAT ATTCTGTGCC TCATGACAGC ACACAAGGTA AAATTATTAC TATACGCAAT 
TCAAATCGAA TCCACTCCAA CGGAGGAGGA AATGGTTCAG ACAGTGAAGG GGATGGCAGC 
TCATTGGAGA GACGTCGCAA GTTTTCAGCA GCAAGGGTTA AGCCACGGCT TTACCGTGAT 
CGTTCCAAAC GTCTGGGGAA GTTCAGCAGT TTCCGCACTA GTTTCTCTAT TGGTAGCGAT 
GATGAGATTG GCACACTCTC AAGGTCAAAA GAGGATGATG TTTCACCACT GAAGACAGAT 
ATAACAAATG AAGAAGGAGA AGATCCCAAA AAGAGAAACA TCCTGAAAAG TCTCCGCAGA 
CCAGGCAAAA AAGCAAGACC AAAGCCTCGT CATTCCATCT CTAAGCCTTT GGAGAGTAAC 
TACTTTGGAG TGCCTCTGGT CAATGTTGTT TCCTTCGAAC GACCTATTCC AGTTTTCATT 
GACAAGTGTA TTCGTTATAT TGAAGCCACA GGGTTAACAA CAGAGGGTAT CTACAGGGTA 
AGTGGGAATA AGGCGGAGAT CGAGGGCATG CAGAGGCAGT TTGAACAGGA CCACAATCTG 
GACTTTGTGG AGAAGGACTT CACTGTGAAC ACAGTTGCCG GGGCAATGAA AAGTTTCTTC 
TCTGAGCTGC CTGATCCTCT GGTTCCTTAC AGTTCACAGG AGGAGCTGGT GGAAGCTTTC 
AAAATTAATG ATCGAGAACA GAGACTTCAC ACAATGAAGG ACGTGTTAAG ACGATTTCCC 
AGGGAGAATT TTGATGTCTT CAAGTATGTA ATGAGCCATC TAAACAAGGT TGGTCAGTGG 
AACAGAGTCA ACTTAATGAC CAGTGAGAAT CTCTCCATCT GTTTCTGGCC AACACTGATG 
CGGCCAGACT TCACTTCTAT GGATGCCCTG ACCGCCACAC GCACCTACCA GACAATCATC 
GAGACGTTTA TCCATCAATG TGCTTTCTTC TTTTACAACC AGCCTCCTGC TGAATCGCCC 
ACTGGCCTAT TCGGCCAGCC TGGACCAATG GTGCCCTGTG GATTGCCCCC ATCCCCCACA 
GCTGCTCCTT CTTATTGCCC TTCGACCCCA CATTTTACCC CTCTTCTTCA GTCCCCGCCT 
CATTCTCCCC CCCAGAGCCC ACACTCCGCC CCTGCAGAAC CACAAACACT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgap35 ( XM_021476554.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021332229.1 Danio rerio Rho GTPase activating protein 35b (arhgap35), transcript variant X2, mRNA.

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CloneID	ODa44355
Clone ID Related Accession (Same CDS sequence)	XM_003198643.5 , XM_009301690.3 , XM_021476553.1 , XM_009301690.2 , XM_003198643.4 , XM_021476554.1
Accession Version	XM_021476554.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4554bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	rho GTPase-activating protein 35
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGG CTAAAAAGCA AGAATCCCGG CTGCCAATCT ACAACCTTGT TGTTGTTGGC 
CTGTCGGGAA CTGAGAAGGA GAAAGGGCAA TGTGGAGTTG GCAAATCTTG CCTTTGTAAT 
CGCTTTGTTC GCCCTAGTGC AGATGACTTC CATCTCGATC ACACCTCTGT GCTAAGTACT 
AGTGACTTTG GTGGCCGGGT TGTCAACAAT GACCATTTCC TGTTCTGGGG GGAGGTTGGG 
AGAGTTTTGG AGGATGGTGC AGACTGCAGA ATGCATGTGG TCGAGCAGAC AGAATTTATT 
GATGACCAGA CTTTCCAGCC CCATCGTAGC ACAGCCTTAC TGCCCTACAT CAAAAGAGCT 
GCCTCCACCA AATTGGCTTC TGCGGAAAAA CTCATGTACT TCTGCACTGA TCAGTTGGGC 
CTGGAGCAGG ACTTCGAACA GAAACAAATG CCTGAAGGCA AGTTGCAGGT CGATGGCTTT 
CTGCTTTGTG TTGATGTCAG TCGTGGTATG AACCGCAACT TTGATGATCA AATGAAATTT 
GTCACCAACT TGTACAATCA CTTAAGCAAG ACAAAAAAGC CTATTGTGCT GGTTCTCACC 
AAGTGTGATG AGGGTGTGGA GCGATACATC AAGGATTCTC ATACCTTTGC CATTACAAAA 
AAGAACCTGC AAGTTGTTGA GACTTCGGCT CGATCCAATA TCAATGTTGA CTTGGCTTTT 
CTTACCTTAA TTCAACTCAT TGATAAGGGA AAAGGTAAGC CTAAGATCAT ACCTTACTTT 
GAGGCATTGA AACACCAGAG TCAACTGATT GCCTCCGCAA AAGACCGCTA TGAATGGTTG 
GTGAACCACA TTGTGAAGAA TCACAATGAA ACTTGGCCAA ACGTCAGCAG ACGTATGCAG 
ACTTCACCCG AATATAAGGA ATATGTGTTT TTAGAAGGCA CAGGTAAAGC TAAGAAACTA 
TTTCAACAGC ACATTCACCG ACTTAAACAA GAGCATACTG AAAGACGTCG TAAAGCATAC 
TTGAGTGCTC TTCCACTCGC CTTAAGTAGC TTGGTGTCGG AGTTGGATGA AATTGAGCAT 
TTAAGCTGGT CTGGGGTGCA AAAAGTGCTT GAGTCTAAAA AAGACTTTGA TCATTGGTTT 
GTGGTACTGG ACGATGCACC ATGGGAAGAC ACACCACATC TTGACAATAT GGAGGATGAC 
CGCATCCCCT TTGATGTGCT AGACACCACG GCTGCTGAAA TCCTCTTTGA TGCACACCTT 
GAACACTTGC GCAATGAATG CAAGCGTGCC CAGATGCGTC ATGAGTTCAA AGGAAAATTA 
GCATCTTCAC CTTTCGTTAC TCCAGGCAAG CCCTGGGAGG AGGCTCGAAG CTTCATCATG 
AATGAAGACT TTTACCAGTG GCTTGAGGAG CCAGAATATT TGGACATTTA TAACCGTCAC 
CAGAAAGCAA TCATTGACAG GGCAAAAGAG GATTTTCAGG AGCTTCTGCT GGAGTACTCT 
GAACTCTTCT ACGAACTTGA GGTGGATGCC AAACCTAGTA AAGAGAAAAT GGGAGCAATT 
CAAGAAGTGT TAGGTGAAGA ACAACGCTTC AAGGCACTTC AGAAACTGCA AGCTGAAAGG 
GATGCTTTAG TACTGAAACA CATCCATTTT GTTTATCACC CTACGAAGGA AACATGCCCA 
AACTGCCCAC ACTGTGTTGA CAGCAAAATT GAGCAAATTC TAGCTTCATG CTTCCCTGCA 
CAATATCCTT CCTTTGGAAA GTCGCATATA ACTGATGCAA AGGTAGATAG GATCAATCTT 
GTTATACTGG GTAAAGATGG ACTTGCTAGA GAATTAGCCA ATGAAATTCG GGCACTGTGT 
ACTAATGATG ATCATTATGT GCTTGAAGGA AGAATGTATG AGCTTGTCCT TCGGCCAATT 
GAGGGCAATG TTAGACTTCC TGTTAATTCG TTTCATACAT CCACATTTAC TCCCCATGGA 
TGCCTGTGTT TGTACAATTC AAAAGAGTCC TTGTCCTATG TTGTAGAAAG CATTGAGAAG 
TTGCGAGAGT CCACACTTGG CAGAAGGGAC AACCATATGG CTCAGCTTCC GTTGTCCCTC 
TTATTGGTAA GCAAAAGAGG TGTTGGAGGA CTGAGTGATA TTGGTGGTGA GACTGCTCAA 
GCGTTAATCT CACAGGGTCA GCAAGTAGCC ATAAAACTGC AGTGTAACTA CCTGGAACCT 
TCCTCCCCAG GCACTGGTTA TGGGCGAAAC GTGAATGAAA AGCAGATCAA CCAGGTTTTG 
AAAGCTCTAC TTGAAACAAG GAGGACTTCT ACATTTCGTA GCTGTTCTCC ACCTGCACCT 
CCACTGCCTC CATGTATTCG AGACTTTCAG CAAGACCAGA GCCCAGAGGC TGATTTGCGT 
ATCGTCATGT GCCTAATGTG TGGAGACTCT TATGACATCG ATCAACTTCT TGCACCATTC 
CTGCTGCCTC AGCATTGCAA GCCAACTTCT CCCCAGACAA GTGGCACTTC AGTGCTGCTA 
GAACAAACAA TTGGTGGCCA CAAGCTTACG ATTGAGCTTT CTCTGCTGTC TTACCACTCT 
TCTTTTGCTA CTCGGAAGAG TCGACTGGTG CATGGCTACA TAGCTGTATA TTCTGCTAGC 
CGCAAGGCTT CTTTTGAAAC CCTGTGTGCC TTTTTGTGTG AGGTTCAGGA CATCATCCCG 
GTACAGATGA TTGCAGTGGG GGAAACACAG ATTGAGCTTC AGGACTCTGA GTCTGCTCGT 
GAATTGTTAG TCCAAGGTGA AGAGCTGGCA CATGAACTTG AAGGACGATT TGGCAGTATT 
GTCTGTGGTC ATGGCGGCGT TGTTGGAGGA CTACACAAAC TAGACCTTTT AGAACAATTT 
TTCACAGAAG TATTAGAAAA GAAAACCATA GTTGAAGCCA GCCATATGTA TGAAAGCATG 
GCAGAAGCCA GTAGCACAAA TGAGAATGTG TATTCGCCAC ACTGTGGCTC CCCAAGCCCT 
GTCACAATGC TGTTAGATTC TGAGGAAGAC ATGGAAGCTT CCCCGCCTTA CCATGATGGC 
ACACTTACCT CTCACGGTGG ATTCAAACTG CCTGACCTGG ACTCAGGTGA CACATTCTCT 
GTGATCTCTG ACCTTAGCAC CTTTGAGAAC AAGCTAAATA ATAAAGTTCC TCCACAAGTG 
AGACCCAAAC CTAGTGTCAC ATTTGACCTT AGAAAGCCAA GTTTTAACCC CTATGTGGAT 
GGAGTCAGCC ACCGCCGTTC CCTCACATCC AATGTGACGT GGCCTCTAGG AGGAGATTAT 
GATCCTTCAG ACTATGCTGA GCCAATGGAT GCTGTCTCTA AGCCACGCCC CAGCTATGAA 
GACAGCATAT ATTCTGTGCC TCATGACAGC ACACAAGGTA AAATTATTAC TATACGCAAT 
TCAAATCGAA TCCACTCCAA CGGAGGAGGA AATGGTTCAG ACAGTGAAGG GGATGGCAGC 
TCATTGGAGA GACGTCGCAA GTTTTCAGCA GCAAGGGTTA AGCCACGGCT TTACCGTGAT 
CGTTCCAAAC GTCTGGGGAA GTTCAGCAGT TTCCGCACTA GTTTCTCTAT TGGTAGCGAT 
GATGAGATTG GCACACTCTC AAGGTCAAAA GAGGATGATG TTTCACCACT GAAGACAGAT 
ATAACAAATG AAGAAGGAGA AGATCCCAAA AAGAGAAACA TCCTGAAAAG TCTCCGCAGA 
CCAGGCAAAA AAGCAAGACC AAAGCCTCGT CATTCCATCT CTAAGCCTTT GGAGAGTAAC 
TACTTTGGAG TGCCTCTGGT CAATGTTGTT TCCTTCGAAC GACCTATTCC AGTTTTCATT 
GACAAGTGTA TTCGTTATAT TGAAGCCACA GGGTTAACAA CAGAGGGTAT CTACAGGGTA 
AGTGGGAATA AGGCGGAGAT CGAGGGCATG CAGAGGCAGT TTGAACAGGA CCACAATCTG 
GACTTTGTGG AGAAGGACTT CACTGTGAAC ACAGTTGCCG GGGCAATGAA AAGTTTCTTC 
TCTGAGCTGC CTGATCCTCT GGTTCCTTAC AGTTCACAGG AGGAGCTGGT GGAAGCTTTC 
AAAATTAATG ATCGAGAACA GAGACTTCAC ACAATGAAGG ACGTGTTAAG ACGATTTCCC 
AGGGAGAATT TTGATGTCTT CAAGTATGTA ATGAGCCATC TAAACAAGGT TGGTCAGTGG 
AACAGAGTCA ACTTAATGAC CAGTGAGAAT CTCTCCATCT GTTTCTGGCC AACACTGATG 
CGGCCAGACT TCACTTCTAT GGATGCCCTG ACCGCCACAC GCACCTACCA GACAATCATC 
GAGACGTTTA TCCATCAATG TGCTTTCTTC TTTTACAACC AGCCTCCTGC TGAATCGCCC 
ACTGGCCTAT TCGGCCAGCC TGGACCAATG GTGCCCTGTG GATTGCCCCC ATCCCCCACA 
GCTGCTCCTT CTTATTGCCC TTCGACCCCA CATTTTACCC CTCTTCTTCA GTCCCCGCCT 
CATTCTCCCC CCCAGAGCCC ACACTCCGCC CCTGCAGAAC CACAAACACT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021332229.1
CDS	1268..5821
Translation	MMMAKKQESRLPIYNLVVVGLSGTEKEKGQCGVGKSCLCNRFVRPSADDFHLDHTSVLSTSDFGGRVVNNDHFLFWGEVGRVLEDGADCRMHVVEQTEFIDDQTFQPHRSTALLPYIKRAASTKLASAEKLMYFCTDQLGLEQDFEQKQMPEGKLQVDGFLLCVDVSRGMNRNFDDQMKFVTNLYNHLSKTKKPIVLVLTKCDEGVERYIKDSHTFAITKKNLQVVETSARSNINVDLAFLTLIQLIDKGKGKPKIIPYFEALKHQSQLIASAKDRYEWLVNHIVKNHNETWPNVSRRMQTSPEYKEYVFLEGTGKAKKLFQQHIHRLKQEHTERRRKAYLSALPLALSSLVSELDEIEHLSWSGVQKVLESKKDFDHWFVVLDDAPWEDTPHLDNMEDDRIPFDVLDTTAAEILFDAHLEHLRNECKRAQMRHEFKGKLASSPFVTPGKPWEEARSFIMNEDFYQWLEEPEYLDIYNRHQKAIIDRAKEDFQELLLEYSELFYELEVDAKPSKEKMGAIQEVLGEEQRFKALQKLQAERDALVLKHIHFVYHPTKETCPNCPHCVDSKIEQILASCFPAQYPSFGKSHITDAKVDRINLVILGKDGLARELANEIRALCTNDDHYVLEGRMYELVLRPIEGNVRLPVNSFHTSTFTPHGCLCLYNSKESLSYVVESIEKLRESTLGRRDNHMAQLPLSLLLVSKRGVGGLSDIGGETAQALISQGQQVAIKLQCNYLEPSSPGTGYGRNVNEKQINQVLKALLETRRTSTFRSCSPPAPPLPPCIRDFQQDQSPEADLRIVMCLMCGDSYDIDQLLAPFLLPQHCKPTSPQTSGTSVLLEQTIGGHKLTIELSLLSYHSSFATRKSRLVHGYIAVYSASRKASFETLCAFLCEVQDIIPVQMIAVGETQIELQDSESARELLVQGEELAHELEGRFGSIVCGHGGVVGGLHKLDLLEQFFTEVLEKKTIVEASHMYESMAEASSTNENVYSPHCGSPSPVTMLLDSEEDMEASPPYHDGTLTSHGGFKLPDLDSGDTFSVISDLSTFENKLNNKVPPQVRPKPSVTFDLRKPSFNPYVDGVSHRRSLTSNVTWPLGGDYDPSDYAEPMDAVSKPRPSYEDSIYSVPHDSTQGKIITIRNSNRIHSNGGGNGSDSEGDGSSLERRRKFSAARVKPRLYRDRSKRLGKFSSFRTSFSIGSDDEIGTLSRSKEDDVSPLKTDITNEEGEDPKKRNILKSLRRPGKKARPKPRHSISKPLESNYFGVPLVNVVSFERPIPVFIDKCIRYIEATGLTTEGIYRVSGNKAEIEGMQRQFEQDHNLDFVEKDFTVNTVAGAMKSFFSELPDPLVPYSSQEELVEAFKINDREQRLHTMKDVLRRFPRENFDVFKYVMSHLNKVGQWNRVNLMTSENLSICFWPTLMRPDFTSMDALTATRTYQTIIETFIHQCAFFFYNQPPAESPTGLFGQPGPMVPCGLPPSPTAAPSYCPSTPHFTPLLQSPPHSPPQSPHSAPAEPQTL

Target ORF information:

RefSeq Version	XM_021476554.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Rho GTPase activating protein 35b (arhgap35), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476554.1

ORF Insert Sequence:

ATGATGATGG CTAAAAAGCA AGAATCCCGG CTGCCAATCT ACAACCTTGT TGTTGTTGGC 
CTGTCGGGAA CTGAGAAGGA GAAAGGGCAA TGTGGAGTTG GCAAATCTTG CCTTTGTAAT 
CGCTTTGTTC GCCCTAGTGC AGATGACTTC CATCTCGATC ACACCTCTGT GCTAAGTACT 
AGTGACTTTG GTGGCCGGGT TGTCAACAAT GACCATTTCC TGTTCTGGGG GGAGGTTGGG 
AGAGTTTTGG AGGATGGTGC AGACTGCAGA ATGCATGTGG TCGAGCAGAC AGAATTTATT 
GATGACCAGA CTTTCCAGCC CCATCGTAGC ACAGCCTTAC TGCCCTACAT CAAAAGAGCT 
GCCTCCACCA AATTGGCTTC TGCGGAAAAA CTCATGTACT TCTGCACTGA TCAGTTGGGC 
CTGGAGCAGG ACTTCGAACA GAAACAAATG CCTGAAGGCA AGTTGCAGGT CGATGGCTTT 
CTGCTTTGTG TTGATGTCAG TCGTGGTATG AACCGCAACT TTGATGATCA AATGAAATTT 
GTCACCAACT TGTACAATCA CTTAAGCAAG ACAAAAAAGC CTATTGTGCT GGTTCTCACC 
AAGTGTGATG AGGGTGTGGA GCGATACATC AAGGATTCTC ATACCTTTGC CATTACAAAA 
AAGAACCTGC AAGTTGTTGA GACTTCGGCT CGATCCAATA TCAATGTTGA CTTGGCTTTT 
CTTACCTTAA TTCAACTCAT TGATAAGGGA AAAGGTAAGC CTAAGATCAT ACCTTACTTT 
GAGGCATTGA AACACCAGAG TCAACTGATT GCCTCCGCAA AAGACCGCTA TGAATGGTTG 
GTGAACCACA TTGTGAAGAA TCACAATGAA ACTTGGCCAA ACGTCAGCAG ACGTATGCAG 
ACTTCACCCG AATATAAGGA ATATGTGTTT TTAGAAGGCA CAGGTAAAGC TAAGAAACTA 
TTTCAACAGC ACATTCACCG ACTTAAACAA GAGCATACTG AAAGACGTCG TAAAGCATAC 
TTGAGTGCTC TTCCACTCGC CTTAAGTAGC TTGGTGTCGG AGTTGGATGA AATTGAGCAT 
TTAAGCTGGT CTGGGGTGCA AAAAGTGCTT GAGTCTAAAA AAGACTTTGA TCATTGGTTT 
GTGGTACTGG ACGATGCACC ATGGGAAGAC ACACCACATC TTGACAATAT GGAGGATGAC 
CGCATCCCCT TTGATGTGCT AGACACCACG GCTGCTGAAA TCCTCTTTGA TGCACACCTT 
GAACACTTGC GCAATGAATG CAAGCGTGCC CAGATGCGTC ATGAGTTCAA AGGAAAATTA 
GCATCTTCAC CTTTCGTTAC TCCAGGCAAG CCCTGGGAGG AGGCTCGAAG CTTCATCATG 
AATGAAGACT TTTACCAGTG GCTTGAGGAG CCAGAATATT TGGACATTTA TAACCGTCAC 
CAGAAAGCAA TCATTGACAG GGCAAAAGAG GATTTTCAGG AGCTTCTGCT GGAGTACTCT 
GAACTCTTCT ACGAACTTGA GGTGGATGCC AAACCTAGTA AAGAGAAAAT GGGAGCAATT 
CAAGAAGTGT TAGGTGAAGA ACAACGCTTC AAGGCACTTC AGAAACTGCA AGCTGAAAGG 
GATGCTTTAG TACTGAAACA CATCCATTTT GTTTATCACC CTACGAAGGA AACATGCCCA 
AACTGCCCAC ACTGTGTTGA CAGCAAAATT GAGCAAATTC TAGCTTCATG CTTCCCTGCA 
CAATATCCTT CCTTTGGAAA GTCGCATATA ACTGATGCAA AGGTAGATAG GATCAATCTT 
GTTATACTGG GTAAAGATGG ACTTGCTAGA GAATTAGCCA ATGAAATTCG GGCACTGTGT 
ACTAATGATG ATCATTATGT GCTTGAAGGA AGAATGTATG AGCTTGTCCT TCGGCCAATT 
GAGGGCAATG TTAGACTTCC TGTTAATTCG TTTCATACAT CCACATTTAC TCCCCATGGA 
TGCCTGTGTT TGTACAATTC AAAAGAGTCC TTGTCCTATG TTGTAGAAAG CATTGAGAAG 
TTGCGAGAGT CCACACTTGG CAGAAGGGAC AACCATATGG CTCAGCTTCC GTTGTCCCTC 
TTATTGGTAA GCAAAAGAGG TGTTGGAGGA CTGAGTGATA TTGGTGGTGA GACTGCTCAA 
GCGTTAATCT CACAGGGTCA GCAAGTAGCC ATAAAACTGC AGTGTAACTA CCTGGAACCT 
TCCTCCCCAG GCACTGGTTA TGGGCGAAAC GTGAATGAAA AGCAGATCAA CCAGGTTTTG 
AAAGCTCTAC TTGAAACAAG GAGGACTTCT ACATTTCGTA GCTGTTCTCC ACCTGCACCT 
CCACTGCCTC CATGTATTCG AGACTTTCAG CAAGACCAGA GCCCAGAGGC TGATTTGCGT 
ATCGTCATGT GCCTAATGTG TGGAGACTCT TATGACATCG ATCAACTTCT TGCACCATTC 
CTGCTGCCTC AGCATTGCAA GCCAACTTCT CCCCAGACAA GTGGCACTTC AGTGCTGCTA 
GAACAAACAA TTGGTGGCCA CAAGCTTACG ATTGAGCTTT CTCTGCTGTC TTACCACTCT 
TCTTTTGCTA CTCGGAAGAG TCGACTGGTG CATGGCTACA TAGCTGTATA TTCTGCTAGC 
CGCAAGGCTT CTTTTGAAAC CCTGTGTGCC TTTTTGTGTG AGGTTCAGGA CATCATCCCG 
GTACAGATGA TTGCAGTGGG GGAAACACAG ATTGAGCTTC AGGACTCTGA GTCTGCTCGT 
GAATTGTTAG TCCAAGGTGA AGAGCTGGCA CATGAACTTG AAGGACGATT TGGCAGTATT 
GTCTGTGGTC ATGGCGGCGT TGTTGGAGGA CTACACAAAC TAGACCTTTT AGAACAATTT 
TTCACAGAAG TATTAGAAAA GAAAACCATA GTTGAAGCCA GCCATATGTA TGAAAGCATG 
GCAGAAGCCA GTAGCACAAA TGAGAATGTG TATTCGCCAC ACTGTGGCTC CCCAAGCCCT 
GTCACAATGC TGTTAGATTC TGAGGAAGAC ATGGAAGCTT CCCCGCCTTA CCATGATGGC 
ACACTTACCT CTCACGGTGG ATTCAAACTG CCTGACCTGG ACTCAGGTGA CACATTCTCT 
GTGATCTCTG ACCTTAGCAC CTTTGAGAAC AAGCTAAATA ATAAAGTTCC TCCACAAGTG 
AGACCCAAAC CTAGTGTCAC ATTTGACCTT AGAAAGCCAA GTTTTAACCC CTATGTGGAT 
GGAGTCAGCC ACCGCCGTTC CCTCACATCC AATGTGACGT GGCCTCTAGG AGGAGATTAT 
GATCCTTCAG ACTATGCTGA GCCAATGGAT GCTGTCTCTA AGCCACGCCC CAGCTATGAA 
GACAGCATAT ATTCTGTGCC TCATGACAGC ACACAAGGTA AAATTATTAC TATACGCAAT 
TCAAATCGAA TCCACTCCAA CGGAGGAGGA AATGGTTCAG ACAGTGAAGG GGATGGCAGC 
TCATTGGAGA GACGTCGCAA GTTTTCAGCA GCAAGGGTTA AGCCACGGCT TTACCGTGAT 
CGTTCCAAAC GTCTGGGGAA GTTCAGCAGT TTCCGCACTA GTTTCTCTAT TGGTAGCGAT 
GATGAGATTG GCACACTCTC AAGGTCAAAA GAGGATGATG TTTCACCACT GAAGACAGAT 
ATAACAAATG AAGAAGGAGA AGATCCCAAA AAGAGAAACA TCCTGAAAAG TCTCCGCAGA 
CCAGGCAAAA AAGCAAGACC AAAGCCTCGT CATTCCATCT CTAAGCCTTT GGAGAGTAAC 
TACTTTGGAG TGCCTCTGGT CAATGTTGTT TCCTTCGAAC GACCTATTCC AGTTTTCATT 
GACAAGTGTA TTCGTTATAT TGAAGCCACA GGGTTAACAA CAGAGGGTAT CTACAGGGTA 
AGTGGGAATA AGGCGGAGAT CGAGGGCATG CAGAGGCAGT TTGAACAGGA CCACAATCTG 
GACTTTGTGG AGAAGGACTT CACTGTGAAC ACAGTTGCCG GGGCAATGAA AAGTTTCTTC 
TCTGAGCTGC CTGATCCTCT GGTTCCTTAC AGTTCACAGG AGGAGCTGGT GGAAGCTTTC 
AAAATTAATG ATCGAGAACA GAGACTTCAC ACAATGAAGG ACGTGTTAAG ACGATTTCCC 
AGGGAGAATT TTGATGTCTT CAAGTATGTA ATGAGCCATC TAAACAAGGT TGGTCAGTGG 
AACAGAGTCA ACTTAATGAC CAGTGAGAAT CTCTCCATCT GTTTCTGGCC AACACTGATG 
CGGCCAGACT TCACTTCTAT GGATGCCCTG ACCGCCACAC GCACCTACCA GACAATCATC 
GAGACGTTTA TCCATCAATG TGCTTTCTTC TTTTACAACC AGCCTCCTGC TGAATCGCCC 
ACTGGCCTAT TCGGCCAGCC TGGACCAATG GTGCCCTGTG GATTGCCCCC ATCCCCCACA 
GCTGCTCCTT CTTATTGCCC TTCGACCCCA CATTTTACCC CTCTTCTTCA GTCCCCGCCT 
CATTCTCCCC CCCAGAGCCC ACACTCCGCC CCTGCAGAAC CACAAACACT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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03-6811-6573
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オンライン注文

arhgap35 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

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