arhgap35 gene cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

mRNA	Protein	Name
XM_004916953.3	XP_004917010.1	rho GTPase-activating protein 35 isoform X1
XM_018096665.1	XP_017952154.1	rho GTPase-activating protein 35 isoform X3
XM_002932597.4	XP_002932643.1	rho GTPase-activating protein 35 isoform X2
XM_004916951.4	XP_004917008.1	rho GTPase-activating protein 35 isoform X1
XM_004916952.4	XP_004917009.1	rho GTPase-activating protein 35 isoform X1
XM_004916951.3	XP_004917008.1	rho GTPase-activating protein 35 isoform X1
XM_004916952.3	XP_004917009.1	rho GTPase-activating protein 35 isoform X1
XM_004916953.4	XP_004917010.1	rho GTPase-activating protein 35 isoform X1
XM_031891234.1	XP_031747094.1	rho GTPase-activating protein 35 isoform X1
XM_002932597.5	XP_002932643.1	rho GTPase-activating protein 35 isoform X2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa14506	XM_004916951.4 Latest version!	Xenopus tropicalis Rho GTPase activating protein 35 (arhgap35), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa14506	XM_004916952.4 Latest version!	Xenopus tropicalis Rho GTPase activating protein 35 (arhgap35), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa14506	XM_031891234.1 Latest version!	Xenopus tropicalis Rho GTPase activating protein 35 (arhgap35), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa14506	XM_004916953.4 Latest version!	Xenopus tropicalis Rho GTPase activating protein 35 (arhgap35), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa14507	XM_002932597.5 Latest version!	Xenopus tropicalis Rho GTPase activating protein 35 (arhgap35), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OXa46324	XM_018096665.1 Latest version!	Xenopus tropicalis Rho GTPase activating protein 35 (arhgap35), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
View Old Accession Versions >>
OXa14506	XM_004916951.3	Xenopus tropicalis Rho GTPase activating protein 35 (arhgap35), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa14506	XM_004916952.3	Xenopus tropicalis Rho GTPase activating protein 35 (arhgap35), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa14506	XM_004916953.3	Xenopus tropicalis Rho GTPase activating protein 35 (arhgap35), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OXa14507	XM_002932597.4	Xenopus tropicalis Rho GTPase activating protein 35 (arhgap35), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
Hide Old Accession Versions >>

arhgap35 ( XM_004916951.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004917008.1 Xenopus tropicalis Rho GTPase activating protein 35 (arhgap35), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa14506
Clone ID Related Accession (Same CDS sequence)	XM_004916953.3 , XM_004916951.4 , XM_004916952.4 , XM_004916951.3 , XM_004916952.3 , XM_004916953.4 , XM_031891234.1
Accession Version	XM_004916951.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4419bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho GTPase-activating protein 35 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004916951.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGG CACGGAAACC AGATGTGCGT GTGCCCACCT ACAGTGTCAG CGTTGTGGGC 
CTTTCTGGCA CAGAGAAAGA GAAAGGCCAA TGTGGCGTTG GAAAATCATG CCTTTGTAAT 
CGATTTGTAA GACCCAGCGC AGATGATTTT CATCTGGACC ACACATCCGT CCTGAGCACT 
AGTGACTTTG GTGGTCGTGT TGTAAACAAT GACCATTTTC TGTACTGGGG AGAGAGTGGA 
CGGATGCCAG AAGACTGTGT AGACTTTAAA GTGCATGTGG TGGAGCAGAC AGAATTTATT 
GATGATCAGA CATTCCAGCC TCACCGTAGT ACTGCCCTAC AGCCTTACAT CAAGCGTGCT 
GCCTCCTGTA AGCTGGCCTC TGCTGAAAAA CTTATGTACT TCTGCACAGA TCAGTTGGGC 
TTGGAACAGG ACTTTGAGCA GAAGCAGATG CCTGAGGGTA AATTGTTGAT TGATGGTTTC 
CTGCTGTGTA TAGATGTTAG TCGTGGAATG AACCGGAACT TTGATGATCA GCTAAAATTT 
GTGTCCAATC TTTACACCCA GCTGGCCAAG ACAAAGAAAC CCATTGTTCT GGTTCTTACA 
AAATGTGATG AAGGAGTAGA ACGTTATATC CGCGATGCTC ATAGTTTTGC CCTTAACAAG 
AAGAACCTGC AAGTTGTAGA GACTTCTGCC CGGTCTAATG TGAATGTGGA TCTGGCCTTC 
ACTACACTAG TACAGCTTAT TGACAAAAGC CGTGGAAAGT CTAAAATTAT TCCTTATTTT 
GAGGCACTAA AGCAGCAGAG CCAACTGATT GCTGCCGCAA AGGACAAATA TGAATGGCTT 
GTGAGCCGAG TAGTAAAGTC TCATAATGAG ACATGGATCA ATCAAAGCAG GCGCATGCAG 
AGCAACATTG AATACCAAGA TTATGTGCAT TTGGAAGGAA CAGACAAAGC TAAGAAACTA 
TTCCATCAGC ACGTGCTACG GCTTAAACAG GAACACATAG AACGACGCAG AAGAAATTAC 
CTCTTAGCTC TCCCACGAGC CTTAGAAAGC CTTGTGCCAG ATCTGGATGA AATAGATCAC 
TTAAGCTGGG TTAAGGTTTC TAATTTGCTG GAATCAAAGG CAGAGTTCCT AAAATTTTTT 
ATTGTCCTGG AAGAGACACC TTGGGACTTC ACAATCCACA TTGACAGCAT TGATGAACGC 
ATACCTTATG ATTTGCTGGA AACAGTGCCT GCGGAAGAGG TCTATGCTGC CCACTTGGTC 
AAGTTAAGGA ATGCCAGGAA AAAGGAAGAA ATGAAGAGAT CTTTCAAGGA GAACTTGATC 
GCTTCTCCTT TCATTACACC AGGTAAGCCT TGGGAAGAGG CTCGGAGTTT TATCATGAGT 
GAAGAATTTT ACCAGTGGCT GGAAGAGCCT GTATATATGG AAATCTACAG CCGACACCAA 
AAAGAGATCA TTGATAAAGC TAAAGAGGAA TTTCAGGAGT TGTTGCTTGA GTATTCAGAG 
CTGTTTTATG AGCTAGAGTT AGATGCCAAA CCCAGTAAAG AGAAAATGGG CGTCATCCAG 
GATGTACTTG GTGAGGAACA ACGTTTCAAA GCATTACAGA AGCTGCAGGC GGAGCGAGAT 
GCCCTGATTC TTAAACACAT TCACTTTGTC TATCATCCAA CCAAGGACAC TTGTCCTAGC 
AACCCTTACT GTGTTGATAC AAGGATTGAG CATATACTCA GTTCCAGGTG CACATATCCC 
TATGACAGAC TACACAAAGA TCCAAATGTA GATAGGATCA ACCTTGTGAT CCTTGGAAAG 
GATGGTTTGG CACGAGAGTT AGCAAATGAG ATCCGAGCCC TCTGTACAAA TGATGATAAA 
TACGTAATTG ATGGAAAGAT GTATGAGTTA TCCCTAAGGC CTATAGAAGG CAATGTGCGG 
CTTCCTGTTA ATTCATTCCA GACATCCACT TTCCTCCCTC ATGGCTGTCT TTGCCTGTAC 
AACTCCAAGG AGTCTCTGTC CTATGTTGTG GAGAGCATTG AGAAAAGCAG AGAATCAAGC 
CTAGGTCGAA AGGAAAATCA CTTGATCCAT TTGCCTCTTA CGTTGATTCT TGTCAACAGG 
AGAGGAGACA CAAGTAGTGA AACTGCACAT AGCCTCATTC AACAGGGCAA ACAGGTTGCC 
AGTAAGCTTC AGTGTTTCTT TCTTGATGCA GCTTCCACTG GAATGGGATA TGGTCGCAAT 
ATCAATGAGA AGCAAATAAG CCTAATCCTC AGAGGGCTAC TGGAGTCAAA AAGAAACTTA 
AACCTTGTCA GCTCCACTCC AAGCATTAAA GATTTGACAG ATTGTGAGCT GCGTATTGTA 
ATGTGCCTCA TGTGTGCAGA CATATTTAAC ATAGAAGATG TGCTGAATGC ACTAAAACCT 
CACACTTACC GTCCATCACA ATGTGGGCTT GGTGGATCAG TTCTACTTGA CCTACCAATT 
GGCAGTCAAA AACGTCGCTT GGAGCTGCTG TTGTTGTCGT ATCATGCCTC CTTTAATGTT 
CGCAAGACTC GTTTAGTGCA CGGCTATATG ATTTTTTACG CAGCTAAGCG CCGTGCTTCC 
CTAGCTATGC TGCGTGCCTT TTTGTGTGAA GTGCAGGACA TTGTCCCTGT GCAGCTTGTT 
GCCTTGACTG AGGGACCTCT AACATTTTTG GATTATGTGG CACGAGAACA GTTACAAGAA 
GGGGAGGAAA TAGCTCAGGA CATTGAAGGT AAATTCCTTA TTCTACCACA AGGGCAGATA 
CAGCCAAAGT TGGATGTGTT TTACCCCTAC TTCAAAGATG TATTGGAGAA AAAAGTAATT 
ATTGAGGCCT CTCACATGTA TGATAATACA GCAGAGGCCT GCAGCACCAC AGAGGAAGTG 
TTTAGCTCAC CCCGTGCAGG CTCACCCCTT TGTAATTCCC ACTTGCCAGA CTCTGAAGAA 
GACCCTGAAC CTTCACCTCA ACACATTTTA TTTCAAAATG AACCTAACAT AGCAGCTCCC 
ATAAAATTTA CCAGAGAACT GGAGGAGACT GTGATGAACA CTTTAGAGAG CAAACTTAAC 
AACAAGGTAC CTCCTCCCCT TAAACCAAAG CCCCCTATTC CATATGAAAT TACCAAGCCT 
GAGCTATCAT ATTTGGATAC AGGGGCCCGG GATGGGCACC GTAAGTCCTT GACTTCCATA 
AACTGGCCCC CTTTGGAGGC CTTTGACCCT TCTGACTATG CTGAACCTAT GGATTCAGTA 
GTGAAGCCAA GAAACCAAGA GGAGAACATT TACTCTGTAC CCCATGATAG CACCCAAGGA 
AAGATCATCA CTATACGTAA TACCAACAAA CCACAATCTA ATGGGAGTGG CAATGGCTCA 
GACAGTGATA TGGACACAAA CTCATTGGAA CGAGCAAGAA AAGCATCCAT TGTCACTAAA 
CCTGTCCTTT ACCGTACCAA ATGTGCTAAA CTTGGGAAAT TCTCTAGCTA CAGGAGTAGT 
TTAAGTGTTG GCAGTGATGA TGAACCTGGA CCAATTCGAA GAAAGGAAGA GGAGACTGGA 
ACACAGGGTA CAAAGGGGGA CAGTACTACG AATTCTTATG AAGCTGATGG AGAGGACCCA 
CGCAAGAGGA ATATTTTGCG CAGTCTGCGA AGGACCACTA AGAAAGTGAA ACCCAAACCT 
CGTCCATCTT TAACCAAGGC GACCTGGGAG AGTAATTACT TTGGTTTCCC TCTGAGTAGT 
GTGGTGACTT CTGAACGACC AATTCCAGTA TTCATTGAGA AGTGTGTAGA GTACATCGAA 
GCCACGGGTG AGGGAATGAC CACAGAAGGC ATATACCGTG TTAGCGGGAA CAAATCTGAG 
ATGGAGTCTC TTCAAAGGCA ATTTGACCAA GATCATAACC TGGACCTGGC AGAAAAGGAT 
TTTACAGTGA ACACTGTGGC TGGAGCCTTG AAAAGTTTCT TCTCTGAATT ACCGGACCCT 
CTGGTGCCAT ACAATATGCA GGCTGAGCTG GTGGAGGCAT ATAAAATAAA TGATCTAGAA 
CATAAGCTCC AAGCTATGAA GGATCTTCTA AAAAAATTTC CAAAGGAAAA TCATGAAATC 
TTCAAATATG TTATATCTCA TTTAAACCGG GTTAGCCAGC ATCATCATAT AAATCTGATG 
ACCAGTGAAA ATTTATCTAT CTGCTTCTGG CCTACACTGA TGCGCCCTGA TTTTACCACT 
ATGGACGCTC TGACTGCAAC TCGCACATAC CAGACAATCA TTGAGCTTTT CATCCAACAG 
TGCCCCTTTT TCTTCTACCA GCGCCTTCCT GTGGACCTGC CTATCCCTAG TTCCCCATCT 
ACTCCACCTC TCACCCAGCC ATCTCCTCCT CAGTCCCCTC CTCTGACGCC TGTATCCCCC 
TCGCAGACCC TGCTTCCTAC GGAGCACAAC ATCCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004917008.1
CDS	551..4969
Translation	MMMARKPDVRVPTYSVSVVGLSGTEKEKGQCGVGKSCLCNRFVRPSADDFHLDHTSVLSTSDFGGRVVNNDHFLYWGESGRMPEDCVDFKVHVVEQTEFIDDQTFQPHRSTALQPYIKRAASCKLASAEKLMYFCTDQLGLEQDFEQKQMPEGKLLIDGFLLCIDVSRGMNRNFDDQLKFVSNLYTQLAKTKKPIVLVLTKCDEGVERYIRDAHSFALNKKNLQVVETSARSNVNVDLAFTTLVQLIDKSRGKSKIIPYFEALKQQSQLIAAAKDKYEWLVSRVVKSHNETWINQSRRMQSNIEYQDYVHLEGTDKAKKLFHQHVLRLKQEHIERRRRNYLLALPRALESLVPDLDEIDHLSWVKVSNLLESKAEFLKFFIVLEETPWDFTIHIDSIDERIPYDLLETVPAEEVYAAHLVKLRNARKKEEMKRSFKENLIASPFITPGKPWEEARSFIMSEEFYQWLEEPVYMEIYSRHQKEIIDKAKEEFQELLLEYSELFYELELDAKPSKEKMGVIQDVLGEEQRFKALQKLQAERDALILKHIHFVYHPTKDTCPSNPYCVDTRIEHILSSRCTYPYDRLHKDPNVDRINLVILGKDGLARELANEIRALCTNDDKYVIDGKMYELSLRPIEGNVRLPVNSFQTSTFLPHGCLCLYNSKESLSYVVESIEKSRESSLGRKENHLIHLPLTLILVNRRGDTSSETAHSLIQQGKQVASKLQCFFLDAASTGMGYGRNINEKQISLILRGLLESKRNLNLVSSTPSIKDLTDCELRIVMCLMCADIFNIEDVLNALKPHTYRPSQCGLGGSVLLDLPIGSQKRRLELLLLSYHASFNVRKTRLVHGYMIFYAAKRRASLAMLRAFLCEVQDIVPVQLVALTEGPLTFLDYVAREQLQEGEEIAQDIEGKFLILPQGQIQPKLDVFYPYFKDVLEKKVIIEASHMYDNTAEACSTTEEVFSSPRAGSPLCNSHLPDSEEDPEPSPQHILFQNEPNIAAPIKFTRELEETVMNTLESKLNNKVPPPLKPKPPIPYEITKPELSYLDTGARDGHRKSLTSINWPPLEAFDPSDYAEPMDSVVKPRNQEENIYSVPHDSTQGKIITIRNTNKPQSNGSGNGSDSDMDTNSLERARKASIVTKPVLYRTKCAKLGKFSSYRSSLSVGSDDEPGPIRRKEEETGTQGTKGDSTTNSYEADGEDPRKRNILRSLRRTTKKVKPKPRPSLTKATWESNYFGFPLSSVVTSERPIPVFIEKCVEYIEATGEGMTTEGIYRVSGNKSEMESLQRQFDQDHNLDLAEKDFTVNTVAGALKSFFSELPDPLVPYNMQAELVEAYKINDLEHKLQAMKDLLKKFPKENHEIFKYVISHLNRVSQHHHINLMTSENLSICFWPTLMRPDFTTMDALTATRTYQTIIELFIQQCPFFFYQRLPVDLPIPSSPSTPPLTQPSPPQSPPLTPVSPSQTLLPTEHNIL

Target ORF information:

RefSeq Version	XM_004916951.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho GTPase activating protein 35 (arhgap35), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004916951.4

ORF Insert Sequence:

ATGATGATGG CACGGAAACC AGATGTGCGT GTGCCCACCT ACAGTGTCAG CGTTGTGGGC 
CTTTCTGGCA CAGAGAAAGA GAAAGGCCAA TGTGGCGTTG GAAAATCATG CCTTTGTAAT 
CGATTTGTAA GACCCAGCGC AGATGATTTT CATCTGGACC ACACATCCGT CCTGAGCACT 
AGTGACTTTG GTGGTCGTGT TGTAAACAAT GACCATTTTC TGTACTGGGG AGAGAGTGGA 
CGGATGCCAG AAGACTGTGT AGACTTTAAA GTGCATGTGG TGGAGCAGAC AGAATTTATT 
GATGATCAGA CATTCCAGCC TCACCGTAGT ACTGCCCTAC AGCCTTACAT CAAGCGTGCT 
GCCTCCTGTA AGCTGGCCTC TGCTGAAAAA CTTATGTACT TCTGCACAGA TCAGTTGGGC 
TTGGAACAGG ACTTTGAGCA GAAGCAGATG CCTGAGGGTA AATTGTTGAT TGATGGTTTC 
CTGCTGTGTA TAGATGTTAG TCGTGGAATG AACCGGAACT TTGATGATCA GCTAAAATTT 
GTGTCCAATC TTTACACCCA GCTGGCCAAG ACAAAGAAAC CCATTGTTCT GGTTCTTACA 
AAATGTGATG AAGGAGTAGA ACGTTATATC CGCGATGCTC ATAGTTTTGC CCTTAACAAG 
AAGAACCTGC AAGTTGTAGA GACTTCTGCC CGGTCTAATG TGAATGTGGA TCTGGCCTTC 
ACTACACTAG TACAGCTTAT TGACAAAAGC CGTGGAAAGT CTAAAATTAT TCCTTATTTT 
GAGGCACTAA AGCAGCAGAG CCAACTGATT GCTGCCGCAA AGGACAAATA TGAATGGCTT 
GTGAGCCGAG TAGTAAAGTC TCATAATGAG ACATGGATCA ATCAAAGCAG GCGCATGCAG 
AGCAACATTG AATACCAAGA TTATGTGCAT TTGGAAGGAA CAGACAAAGC TAAGAAACTA 
TTCCATCAGC ACGTGCTACG GCTTAAACAG GAACACATAG AACGACGCAG AAGAAATTAC 
CTCTTAGCTC TCCCACGAGC CTTAGAAAGC CTTGTGCCAG ATCTGGATGA AATAGATCAC 
TTAAGCTGGG TTAAGGTTTC TAATTTGCTG GAATCAAAGG CAGAGTTCCT AAAATTTTTT 
ATTGTCCTGG AAGAGACACC TTGGGACTTC ACAATCCACA TTGACAGCAT TGATGAACGC 
ATACCTTATG ATTTGCTGGA AACAGTGCCT GCGGAAGAGG TCTATGCTGC CCACTTGGTC 
AAGTTAAGGA ATGCCAGGAA AAAGGAAGAA ATGAAGAGAT CTTTCAAGGA GAACTTGATC 
GCTTCTCCTT TCATTACACC AGGTAAGCCT TGGGAAGAGG CTCGGAGTTT TATCATGAGT 
GAAGAATTTT ACCAGTGGCT GGAAGAGCCT GTATATATGG AAATCTACAG CCGACACCAA 
AAAGAGATCA TTGATAAAGC TAAAGAGGAA TTTCAGGAGT TGTTGCTTGA GTATTCAGAG 
CTGTTTTATG AGCTAGAGTT AGATGCCAAA CCCAGTAAAG AGAAAATGGG CGTCATCCAG 
GATGTACTTG GTGAGGAACA ACGTTTCAAA GCATTACAGA AGCTGCAGGC GGAGCGAGAT 
GCCCTGATTC TTAAACACAT TCACTTTGTC TATCATCCAA CCAAGGACAC TTGTCCTAGC 
AACCCTTACT GTGTTGATAC AAGGATTGAG CATATACTCA GTTCCAGGTG CACATATCCC 
TATGACAGAC TACACAAAGA TCCAAATGTA GATAGGATCA ACCTTGTGAT CCTTGGAAAG 
GATGGTTTGG CACGAGAGTT AGCAAATGAG ATCCGAGCCC TCTGTACAAA TGATGATAAA 
TACGTAATTG ATGGAAAGAT GTATGAGTTA TCCCTAAGGC CTATAGAAGG CAATGTGCGG 
CTTCCTGTTA ATTCATTCCA GACATCCACT TTCCTCCCTC ATGGCTGTCT TTGCCTGTAC 
AACTCCAAGG AGTCTCTGTC CTATGTTGTG GAGAGCATTG AGAAAAGCAG AGAATCAAGC 
CTAGGTCGAA AGGAAAATCA CTTGATCCAT TTGCCTCTTA CGTTGATTCT TGTCAACAGG 
AGAGGAGACA CAAGTAGTGA AACTGCACAT AGCCTCATTC AACAGGGCAA ACAGGTTGCC 
AGTAAGCTTC AGTGTTTCTT TCTTGATGCA GCTTCCACTG GAATGGGATA TGGTCGCAAT 
ATCAATGAGA AGCAAATAAG CCTAATCCTC AGAGGGCTAC TGGAGTCAAA AAGAAACTTA 
AACCTTGTCA GCTCCACTCC AAGCATTAAA GATTTGACAG ATTGTGAGCT GCGTATTGTA 
ATGTGCCTCA TGTGTGCAGA CATATTTAAC ATAGAAGATG TGCTGAATGC ACTAAAACCT 
CACACTTACC GTCCATCACA ATGTGGGCTT GGTGGATCAG TTCTACTTGA CCTACCAATT 
GGCAGTCAAA AACGTCGCTT GGAGCTGCTG TTGTTGTCGT ATCATGCCTC CTTTAATGTT 
CGCAAGACTC GTTTAGTGCA CGGCTATATG ATTTTTTACG CAGCTAAGCG CCGTGCTTCC 
CTAGCTATGC TGCGTGCCTT TTTGTGTGAA GTGCAGGACA TTGTCCCTGT GCAGCTTGTT 
GCCTTGACTG AGGGACCTCT AACATTTTTG GATTATGTGG CACGAGAACA GTTACAAGAA 
GGGGAGGAAA TAGCTCAGGA CATTGAAGGT AAATTCCTTA TTCTACCACA AGGGCAGATA 
CAGCCAAAGT TGGATGTGTT TTACCCCTAC TTCAAAGATG TATTGGAGAA AAAAGTAATT 
ATTGAGGCCT CTCACATGTA TGATAATACA GCAGAGGCCT GCAGCACCAC AGAGGAAGTG 
TTTAGCTCAC CCCGTGCAGG CTCACCCCTT TGTAATTCCC ACTTGCCAGA CTCTGAAGAA 
GACCCTGAAC CTTCACCTCA ACACATTTTA TTTCAAAATG AACCTAACAT AGCAGCTCCC 
ATAAAATTTA CCAGAGAACT GGAGGAGACT GTGATGAACA CTTTAGAGAG CAAACTTAAC 
AACAAGGTAC CTCCTCCCCT TAAACCAAAG CCCCCTATTC CATATGAAAT TACCAAGCCT 
GAGCTATCAT ATTTGGATAC AGGGGCCCGG GATGGGCACC GTAAGTCCTT GACTTCCATA 
AACTGGCCCC CTTTGGAGGC CTTTGACCCT TCTGACTATG CTGAACCTAT GGATTCAGTA 
GTGAAGCCAA GAAACCAAGA GGAGAACATT TACTCTGTAC CCCATGATAG CACCCAAGGA 
AAGATCATCA CTATACGTAA TACCAACAAA CCACAATCTA ATGGGAGTGG CAATGGCTCA 
GACAGTGATA TGGACACAAA CTCATTGGAA CGAGCAAGAA AAGCATCCAT TGTCACTAAA 
CCTGTCCTTT ACCGTACCAA ATGTGCTAAA CTTGGGAAAT TCTCTAGCTA CAGGAGTAGT 
TTAAGTGTTG GCAGTGATGA TGAACCTGGA CCAATTCGAA GAAAGGAAGA GGAGACTGGA 
ACACAGGGTA CAAAGGGGGA CAGTACTACG AATTCTTATG AAGCTGATGG AGAGGACCCA 
CGCAAGAGGA ATATTTTGCG CAGTCTGCGA AGGACCACTA AGAAAGTGAA ACCCAAACCT 
CGTCCATCTT TAACCAAGGC GACCTGGGAG AGTAATTACT TTGGTTTCCC TCTGAGTAGT 
GTGGTGACTT CTGAACGACC AATTCCAGTA TTCATTGAGA AGTGTGTAGA GTACATCGAA 
GCCACGGGTG AGGGAATGAC CACAGAAGGC ATATACCGTG TTAGCGGGAA CAAATCTGAG 
ATGGAGTCTC TTCAAAGGCA ATTTGACCAA GATCATAACC TGGACCTGGC AGAAAAGGAT 
TTTACAGTGA ACACTGTGGC TGGAGCCTTG AAAAGTTTCT TCTCTGAATT ACCGGACCCT 
CTGGTGCCAT ACAATATGCA GGCTGAGCTG GTGGAGGCAT ATAAAATAAA TGATCTAGAA 
CATAAGCTCC AAGCTATGAA GGATCTTCTA AAAAAATTTC CAAAGGAAAA TCATGAAATC 
TTCAAATATG TTATATCTCA TTTAAACCGG GTTAGCCAGC ATCATCATAT AAATCTGATG 
ACCAGTGAAA ATTTATCTAT CTGCTTCTGG CCTACACTGA TGCGCCCTGA TTTTACCACT 
ATGGACGCTC TGACTGCAAC TCGCACATAC CAGACAATCA TTGAGCTTTT CATCCAACAG 
TGCCCCTTTT TCTTCTACCA GCGCCTTCCT GTGGACCTGC CTATCCCTAG TTCCCCATCT 
ACTCCACCTC TCACCCAGCC ATCTCCTCCT CAGTCCCCTC CTCTGACGCC TGTATCCCCC 
TCGCAGACCC TGCTTCCTAC GGAGCACAAC ATCCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgap35 ( XM_004916952.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004917009.1 Xenopus tropicalis Rho GTPase activating protein 35 (arhgap35), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa14506
Clone ID Related Accession (Same CDS sequence)	XM_004916953.3 , XM_004916951.4 , XM_004916952.4 , XM_004916951.3 , XM_004916952.3 , XM_004916953.4 , XM_031891234.1
Accession Version	XM_004916952.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4419bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho GTPase-activating protein 35 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004916952.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGG CACGGAAACC AGATGTGCGT GTGCCCACCT ACAGTGTCAG CGTTGTGGGC 
CTTTCTGGCA CAGAGAAAGA GAAAGGCCAA TGTGGCGTTG GAAAATCATG CCTTTGTAAT 
CGATTTGTAA GACCCAGCGC AGATGATTTT CATCTGGACC ACACATCCGT CCTGAGCACT 
AGTGACTTTG GTGGTCGTGT TGTAAACAAT GACCATTTTC TGTACTGGGG AGAGAGTGGA 
CGGATGCCAG AAGACTGTGT AGACTTTAAA GTGCATGTGG TGGAGCAGAC AGAATTTATT 
GATGATCAGA CATTCCAGCC TCACCGTAGT ACTGCCCTAC AGCCTTACAT CAAGCGTGCT 
GCCTCCTGTA AGCTGGCCTC TGCTGAAAAA CTTATGTACT TCTGCACAGA TCAGTTGGGC 
TTGGAACAGG ACTTTGAGCA GAAGCAGATG CCTGAGGGTA AATTGTTGAT TGATGGTTTC 
CTGCTGTGTA TAGATGTTAG TCGTGGAATG AACCGGAACT TTGATGATCA GCTAAAATTT 
GTGTCCAATC TTTACACCCA GCTGGCCAAG ACAAAGAAAC CCATTGTTCT GGTTCTTACA 
AAATGTGATG AAGGAGTAGA ACGTTATATC CGCGATGCTC ATAGTTTTGC CCTTAACAAG 
AAGAACCTGC AAGTTGTAGA GACTTCTGCC CGGTCTAATG TGAATGTGGA TCTGGCCTTC 
ACTACACTAG TACAGCTTAT TGACAAAAGC CGTGGAAAGT CTAAAATTAT TCCTTATTTT 
GAGGCACTAA AGCAGCAGAG CCAACTGATT GCTGCCGCAA AGGACAAATA TGAATGGCTT 
GTGAGCCGAG TAGTAAAGTC TCATAATGAG ACATGGATCA ATCAAAGCAG GCGCATGCAG 
AGCAACATTG AATACCAAGA TTATGTGCAT TTGGAAGGAA CAGACAAAGC TAAGAAACTA 
TTCCATCAGC ACGTGCTACG GCTTAAACAG GAACACATAG AACGACGCAG AAGAAATTAC 
CTCTTAGCTC TCCCACGAGC CTTAGAAAGC CTTGTGCCAG ATCTGGATGA AATAGATCAC 
TTAAGCTGGG TTAAGGTTTC TAATTTGCTG GAATCAAAGG CAGAGTTCCT AAAATTTTTT 
ATTGTCCTGG AAGAGACACC TTGGGACTTC ACAATCCACA TTGACAGCAT TGATGAACGC 
ATACCTTATG ATTTGCTGGA AACAGTGCCT GCGGAAGAGG TCTATGCTGC CCACTTGGTC 
AAGTTAAGGA ATGCCAGGAA AAAGGAAGAA ATGAAGAGAT CTTTCAAGGA GAACTTGATC 
GCTTCTCCTT TCATTACACC AGGTAAGCCT TGGGAAGAGG CTCGGAGTTT TATCATGAGT 
GAAGAATTTT ACCAGTGGCT GGAAGAGCCT GTATATATGG AAATCTACAG CCGACACCAA 
AAAGAGATCA TTGATAAAGC TAAAGAGGAA TTTCAGGAGT TGTTGCTTGA GTATTCAGAG 
CTGTTTTATG AGCTAGAGTT AGATGCCAAA CCCAGTAAAG AGAAAATGGG CGTCATCCAG 
GATGTACTTG GTGAGGAACA ACGTTTCAAA GCATTACAGA AGCTGCAGGC GGAGCGAGAT 
GCCCTGATTC TTAAACACAT TCACTTTGTC TATCATCCAA CCAAGGACAC TTGTCCTAGC 
AACCCTTACT GTGTTGATAC AAGGATTGAG CATATACTCA GTTCCAGGTG CACATATCCC 
TATGACAGAC TACACAAAGA TCCAAATGTA GATAGGATCA ACCTTGTGAT CCTTGGAAAG 
GATGGTTTGG CACGAGAGTT AGCAAATGAG ATCCGAGCCC TCTGTACAAA TGATGATAAA 
TACGTAATTG ATGGAAAGAT GTATGAGTTA TCCCTAAGGC CTATAGAAGG CAATGTGCGG 
CTTCCTGTTA ATTCATTCCA GACATCCACT TTCCTCCCTC ATGGCTGTCT TTGCCTGTAC 
AACTCCAAGG AGTCTCTGTC CTATGTTGTG GAGAGCATTG AGAAAAGCAG AGAATCAAGC 
CTAGGTCGAA AGGAAAATCA CTTGATCCAT TTGCCTCTTA CGTTGATTCT TGTCAACAGG 
AGAGGAGACA CAAGTAGTGA AACTGCACAT AGCCTCATTC AACAGGGCAA ACAGGTTGCC 
AGTAAGCTTC AGTGTTTCTT TCTTGATGCA GCTTCCACTG GAATGGGATA TGGTCGCAAT 
ATCAATGAGA AGCAAATAAG CCTAATCCTC AGAGGGCTAC TGGAGTCAAA AAGAAACTTA 
AACCTTGTCA GCTCCACTCC AAGCATTAAA GATTTGACAG ATTGTGAGCT GCGTATTGTA 
ATGTGCCTCA TGTGTGCAGA CATATTTAAC ATAGAAGATG TGCTGAATGC ACTAAAACCT 
CACACTTACC GTCCATCACA ATGTGGGCTT GGTGGATCAG TTCTACTTGA CCTACCAATT 
GGCAGTCAAA AACGTCGCTT GGAGCTGCTG TTGTTGTCGT ATCATGCCTC CTTTAATGTT 
CGCAAGACTC GTTTAGTGCA CGGCTATATG ATTTTTTACG CAGCTAAGCG CCGTGCTTCC 
CTAGCTATGC TGCGTGCCTT TTTGTGTGAA GTGCAGGACA TTGTCCCTGT GCAGCTTGTT 
GCCTTGACTG AGGGACCTCT AACATTTTTG GATTATGTGG CACGAGAACA GTTACAAGAA 
GGGGAGGAAA TAGCTCAGGA CATTGAAGGT AAATTCCTTA TTCTACCACA AGGGCAGATA 
CAGCCAAAGT TGGATGTGTT TTACCCCTAC TTCAAAGATG TATTGGAGAA AAAAGTAATT 
ATTGAGGCCT CTCACATGTA TGATAATACA GCAGAGGCCT GCAGCACCAC AGAGGAAGTG 
TTTAGCTCAC CCCGTGCAGG CTCACCCCTT TGTAATTCCC ACTTGCCAGA CTCTGAAGAA 
GACCCTGAAC CTTCACCTCA ACACATTTTA TTTCAAAATG AACCTAACAT AGCAGCTCCC 
ATAAAATTTA CCAGAGAACT GGAGGAGACT GTGATGAACA CTTTAGAGAG CAAACTTAAC 
AACAAGGTAC CTCCTCCCCT TAAACCAAAG CCCCCTATTC CATATGAAAT TACCAAGCCT 
GAGCTATCAT ATTTGGATAC AGGGGCCCGG GATGGGCACC GTAAGTCCTT GACTTCCATA 
AACTGGCCCC CTTTGGAGGC CTTTGACCCT TCTGACTATG CTGAACCTAT GGATTCAGTA 
GTGAAGCCAA GAAACCAAGA GGAGAACATT TACTCTGTAC CCCATGATAG CACCCAAGGA 
AAGATCATCA CTATACGTAA TACCAACAAA CCACAATCTA ATGGGAGTGG CAATGGCTCA 
GACAGTGATA TGGACACAAA CTCATTGGAA CGAGCAAGAA AAGCATCCAT TGTCACTAAA 
CCTGTCCTTT ACCGTACCAA ATGTGCTAAA CTTGGGAAAT TCTCTAGCTA CAGGAGTAGT 
TTAAGTGTTG GCAGTGATGA TGAACCTGGA CCAATTCGAA GAAAGGAAGA GGAGACTGGA 
ACACAGGGTA CAAAGGGGGA CAGTACTACG AATTCTTATG AAGCTGATGG AGAGGACCCA 
CGCAAGAGGA ATATTTTGCG CAGTCTGCGA AGGACCACTA AGAAAGTGAA ACCCAAACCT 
CGTCCATCTT TAACCAAGGC GACCTGGGAG AGTAATTACT TTGGTTTCCC TCTGAGTAGT 
GTGGTGACTT CTGAACGACC AATTCCAGTA TTCATTGAGA AGTGTGTAGA GTACATCGAA 
GCCACGGGTG AGGGAATGAC CACAGAAGGC ATATACCGTG TTAGCGGGAA CAAATCTGAG 
ATGGAGTCTC TTCAAAGGCA ATTTGACCAA GATCATAACC TGGACCTGGC AGAAAAGGAT 
TTTACAGTGA ACACTGTGGC TGGAGCCTTG AAAAGTTTCT TCTCTGAATT ACCGGACCCT 
CTGGTGCCAT ACAATATGCA GGCTGAGCTG GTGGAGGCAT ATAAAATAAA TGATCTAGAA 
CATAAGCTCC AAGCTATGAA GGATCTTCTA AAAAAATTTC CAAAGGAAAA TCATGAAATC 
TTCAAATATG TTATATCTCA TTTAAACCGG GTTAGCCAGC ATCATCATAT AAATCTGATG 
ACCAGTGAAA ATTTATCTAT CTGCTTCTGG CCTACACTGA TGCGCCCTGA TTTTACCACT 
ATGGACGCTC TGACTGCAAC TCGCACATAC CAGACAATCA TTGAGCTTTT CATCCAACAG 
TGCCCCTTTT TCTTCTACCA GCGCCTTCCT GTGGACCTGC CTATCCCTAG TTCCCCATCT 
ACTCCACCTC TCACCCAGCC ATCTCCTCCT CAGTCCCCTC CTCTGACGCC TGTATCCCCC 
TCGCAGACCC TGCTTCCTAC GGAGCACAAC ATCCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004917009.1
CDS	611..5029
Translation	MMMARKPDVRVPTYSVSVVGLSGTEKEKGQCGVGKSCLCNRFVRPSADDFHLDHTSVLSTSDFGGRVVNNDHFLYWGESGRMPEDCVDFKVHVVEQTEFIDDQTFQPHRSTALQPYIKRAASCKLASAEKLMYFCTDQLGLEQDFEQKQMPEGKLLIDGFLLCIDVSRGMNRNFDDQLKFVSNLYTQLAKTKKPIVLVLTKCDEGVERYIRDAHSFALNKKNLQVVETSARSNVNVDLAFTTLVQLIDKSRGKSKIIPYFEALKQQSQLIAAAKDKYEWLVSRVVKSHNETWINQSRRMQSNIEYQDYVHLEGTDKAKKLFHQHVLRLKQEHIERRRRNYLLALPRALESLVPDLDEIDHLSWVKVSNLLESKAEFLKFFIVLEETPWDFTIHIDSIDERIPYDLLETVPAEEVYAAHLVKLRNARKKEEMKRSFKENLIASPFITPGKPWEEARSFIMSEEFYQWLEEPVYMEIYSRHQKEIIDKAKEEFQELLLEYSELFYELELDAKPSKEKMGVIQDVLGEEQRFKALQKLQAERDALILKHIHFVYHPTKDTCPSNPYCVDTRIEHILSSRCTYPYDRLHKDPNVDRINLVILGKDGLARELANEIRALCTNDDKYVIDGKMYELSLRPIEGNVRLPVNSFQTSTFLPHGCLCLYNSKESLSYVVESIEKSRESSLGRKENHLIHLPLTLILVNRRGDTSSETAHSLIQQGKQVASKLQCFFLDAASTGMGYGRNINEKQISLILRGLLESKRNLNLVSSTPSIKDLTDCELRIVMCLMCADIFNIEDVLNALKPHTYRPSQCGLGGSVLLDLPIGSQKRRLELLLLSYHASFNVRKTRLVHGYMIFYAAKRRASLAMLRAFLCEVQDIVPVQLVALTEGPLTFLDYVAREQLQEGEEIAQDIEGKFLILPQGQIQPKLDVFYPYFKDVLEKKVIIEASHMYDNTAEACSTTEEVFSSPRAGSPLCNSHLPDSEEDPEPSPQHILFQNEPNIAAPIKFTRELEETVMNTLESKLNNKVPPPLKPKPPIPYEITKPELSYLDTGARDGHRKSLTSINWPPLEAFDPSDYAEPMDSVVKPRNQEENIYSVPHDSTQGKIITIRNTNKPQSNGSGNGSDSDMDTNSLERARKASIVTKPVLYRTKCAKLGKFSSYRSSLSVGSDDEPGPIRRKEEETGTQGTKGDSTTNSYEADGEDPRKRNILRSLRRTTKKVKPKPRPSLTKATWESNYFGFPLSSVVTSERPIPVFIEKCVEYIEATGEGMTTEGIYRVSGNKSEMESLQRQFDQDHNLDLAEKDFTVNTVAGALKSFFSELPDPLVPYNMQAELVEAYKINDLEHKLQAMKDLLKKFPKENHEIFKYVISHLNRVSQHHHINLMTSENLSICFWPTLMRPDFTTMDALTATRTYQTIIELFIQQCPFFFYQRLPVDLPIPSSPSTPPLTQPSPPQSPPLTPVSPSQTLLPTEHNIL

Target ORF information:

RefSeq Version	XM_004916952.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho GTPase activating protein 35 (arhgap35), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004916952.4

ORF Insert Sequence:

ATGATGATGG CACGGAAACC AGATGTGCGT GTGCCCACCT ACAGTGTCAG CGTTGTGGGC 
CTTTCTGGCA CAGAGAAAGA GAAAGGCCAA TGTGGCGTTG GAAAATCATG CCTTTGTAAT 
CGATTTGTAA GACCCAGCGC AGATGATTTT CATCTGGACC ACACATCCGT CCTGAGCACT 
AGTGACTTTG GTGGTCGTGT TGTAAACAAT GACCATTTTC TGTACTGGGG AGAGAGTGGA 
CGGATGCCAG AAGACTGTGT AGACTTTAAA GTGCATGTGG TGGAGCAGAC AGAATTTATT 
GATGATCAGA CATTCCAGCC TCACCGTAGT ACTGCCCTAC AGCCTTACAT CAAGCGTGCT 
GCCTCCTGTA AGCTGGCCTC TGCTGAAAAA CTTATGTACT TCTGCACAGA TCAGTTGGGC 
TTGGAACAGG ACTTTGAGCA GAAGCAGATG CCTGAGGGTA AATTGTTGAT TGATGGTTTC 
CTGCTGTGTA TAGATGTTAG TCGTGGAATG AACCGGAACT TTGATGATCA GCTAAAATTT 
GTGTCCAATC TTTACACCCA GCTGGCCAAG ACAAAGAAAC CCATTGTTCT GGTTCTTACA 
AAATGTGATG AAGGAGTAGA ACGTTATATC CGCGATGCTC ATAGTTTTGC CCTTAACAAG 
AAGAACCTGC AAGTTGTAGA GACTTCTGCC CGGTCTAATG TGAATGTGGA TCTGGCCTTC 
ACTACACTAG TACAGCTTAT TGACAAAAGC CGTGGAAAGT CTAAAATTAT TCCTTATTTT 
GAGGCACTAA AGCAGCAGAG CCAACTGATT GCTGCCGCAA AGGACAAATA TGAATGGCTT 
GTGAGCCGAG TAGTAAAGTC TCATAATGAG ACATGGATCA ATCAAAGCAG GCGCATGCAG 
AGCAACATTG AATACCAAGA TTATGTGCAT TTGGAAGGAA CAGACAAAGC TAAGAAACTA 
TTCCATCAGC ACGTGCTACG GCTTAAACAG GAACACATAG AACGACGCAG AAGAAATTAC 
CTCTTAGCTC TCCCACGAGC CTTAGAAAGC CTTGTGCCAG ATCTGGATGA AATAGATCAC 
TTAAGCTGGG TTAAGGTTTC TAATTTGCTG GAATCAAAGG CAGAGTTCCT AAAATTTTTT 
ATTGTCCTGG AAGAGACACC TTGGGACTTC ACAATCCACA TTGACAGCAT TGATGAACGC 
ATACCTTATG ATTTGCTGGA AACAGTGCCT GCGGAAGAGG TCTATGCTGC CCACTTGGTC 
AAGTTAAGGA ATGCCAGGAA AAAGGAAGAA ATGAAGAGAT CTTTCAAGGA GAACTTGATC 
GCTTCTCCTT TCATTACACC AGGTAAGCCT TGGGAAGAGG CTCGGAGTTT TATCATGAGT 
GAAGAATTTT ACCAGTGGCT GGAAGAGCCT GTATATATGG AAATCTACAG CCGACACCAA 
AAAGAGATCA TTGATAAAGC TAAAGAGGAA TTTCAGGAGT TGTTGCTTGA GTATTCAGAG 
CTGTTTTATG AGCTAGAGTT AGATGCCAAA CCCAGTAAAG AGAAAATGGG CGTCATCCAG 
GATGTACTTG GTGAGGAACA ACGTTTCAAA GCATTACAGA AGCTGCAGGC GGAGCGAGAT 
GCCCTGATTC TTAAACACAT TCACTTTGTC TATCATCCAA CCAAGGACAC TTGTCCTAGC 
AACCCTTACT GTGTTGATAC AAGGATTGAG CATATACTCA GTTCCAGGTG CACATATCCC 
TATGACAGAC TACACAAAGA TCCAAATGTA GATAGGATCA ACCTTGTGAT CCTTGGAAAG 
GATGGTTTGG CACGAGAGTT AGCAAATGAG ATCCGAGCCC TCTGTACAAA TGATGATAAA 
TACGTAATTG ATGGAAAGAT GTATGAGTTA TCCCTAAGGC CTATAGAAGG CAATGTGCGG 
CTTCCTGTTA ATTCATTCCA GACATCCACT TTCCTCCCTC ATGGCTGTCT TTGCCTGTAC 
AACTCCAAGG AGTCTCTGTC CTATGTTGTG GAGAGCATTG AGAAAAGCAG AGAATCAAGC 
CTAGGTCGAA AGGAAAATCA CTTGATCCAT TTGCCTCTTA CGTTGATTCT TGTCAACAGG 
AGAGGAGACA CAAGTAGTGA AACTGCACAT AGCCTCATTC AACAGGGCAA ACAGGTTGCC 
AGTAAGCTTC AGTGTTTCTT TCTTGATGCA GCTTCCACTG GAATGGGATA TGGTCGCAAT 
ATCAATGAGA AGCAAATAAG CCTAATCCTC AGAGGGCTAC TGGAGTCAAA AAGAAACTTA 
AACCTTGTCA GCTCCACTCC AAGCATTAAA GATTTGACAG ATTGTGAGCT GCGTATTGTA 
ATGTGCCTCA TGTGTGCAGA CATATTTAAC ATAGAAGATG TGCTGAATGC ACTAAAACCT 
CACACTTACC GTCCATCACA ATGTGGGCTT GGTGGATCAG TTCTACTTGA CCTACCAATT 
GGCAGTCAAA AACGTCGCTT GGAGCTGCTG TTGTTGTCGT ATCATGCCTC CTTTAATGTT 
CGCAAGACTC GTTTAGTGCA CGGCTATATG ATTTTTTACG CAGCTAAGCG CCGTGCTTCC 
CTAGCTATGC TGCGTGCCTT TTTGTGTGAA GTGCAGGACA TTGTCCCTGT GCAGCTTGTT 
GCCTTGACTG AGGGACCTCT AACATTTTTG GATTATGTGG CACGAGAACA GTTACAAGAA 
GGGGAGGAAA TAGCTCAGGA CATTGAAGGT AAATTCCTTA TTCTACCACA AGGGCAGATA 
CAGCCAAAGT TGGATGTGTT TTACCCCTAC TTCAAAGATG TATTGGAGAA AAAAGTAATT 
ATTGAGGCCT CTCACATGTA TGATAATACA GCAGAGGCCT GCAGCACCAC AGAGGAAGTG 
TTTAGCTCAC CCCGTGCAGG CTCACCCCTT TGTAATTCCC ACTTGCCAGA CTCTGAAGAA 
GACCCTGAAC CTTCACCTCA ACACATTTTA TTTCAAAATG AACCTAACAT AGCAGCTCCC 
ATAAAATTTA CCAGAGAACT GGAGGAGACT GTGATGAACA CTTTAGAGAG CAAACTTAAC 
AACAAGGTAC CTCCTCCCCT TAAACCAAAG CCCCCTATTC CATATGAAAT TACCAAGCCT 
GAGCTATCAT ATTTGGATAC AGGGGCCCGG GATGGGCACC GTAAGTCCTT GACTTCCATA 
AACTGGCCCC CTTTGGAGGC CTTTGACCCT TCTGACTATG CTGAACCTAT GGATTCAGTA 
GTGAAGCCAA GAAACCAAGA GGAGAACATT TACTCTGTAC CCCATGATAG CACCCAAGGA 
AAGATCATCA CTATACGTAA TACCAACAAA CCACAATCTA ATGGGAGTGG CAATGGCTCA 
GACAGTGATA TGGACACAAA CTCATTGGAA CGAGCAAGAA AAGCATCCAT TGTCACTAAA 
CCTGTCCTTT ACCGTACCAA ATGTGCTAAA CTTGGGAAAT TCTCTAGCTA CAGGAGTAGT 
TTAAGTGTTG GCAGTGATGA TGAACCTGGA CCAATTCGAA GAAAGGAAGA GGAGACTGGA 
ACACAGGGTA CAAAGGGGGA CAGTACTACG AATTCTTATG AAGCTGATGG AGAGGACCCA 
CGCAAGAGGA ATATTTTGCG CAGTCTGCGA AGGACCACTA AGAAAGTGAA ACCCAAACCT 
CGTCCATCTT TAACCAAGGC GACCTGGGAG AGTAATTACT TTGGTTTCCC TCTGAGTAGT 
GTGGTGACTT CTGAACGACC AATTCCAGTA TTCATTGAGA AGTGTGTAGA GTACATCGAA 
GCCACGGGTG AGGGAATGAC CACAGAAGGC ATATACCGTG TTAGCGGGAA CAAATCTGAG 
ATGGAGTCTC TTCAAAGGCA ATTTGACCAA GATCATAACC TGGACCTGGC AGAAAAGGAT 
TTTACAGTGA ACACTGTGGC TGGAGCCTTG AAAAGTTTCT TCTCTGAATT ACCGGACCCT 
CTGGTGCCAT ACAATATGCA GGCTGAGCTG GTGGAGGCAT ATAAAATAAA TGATCTAGAA 
CATAAGCTCC AAGCTATGAA GGATCTTCTA AAAAAATTTC CAAAGGAAAA TCATGAAATC 
TTCAAATATG TTATATCTCA TTTAAACCGG GTTAGCCAGC ATCATCATAT AAATCTGATG 
ACCAGTGAAA ATTTATCTAT CTGCTTCTGG CCTACACTGA TGCGCCCTGA TTTTACCACT 
ATGGACGCTC TGACTGCAAC TCGCACATAC CAGACAATCA TTGAGCTTTT CATCCAACAG 
TGCCCCTTTT TCTTCTACCA GCGCCTTCCT GTGGACCTGC CTATCCCTAG TTCCCCATCT 
ACTCCACCTC TCACCCAGCC ATCTCCTCCT CAGTCCCCTC CTCTGACGCC TGTATCCCCC 
TCGCAGACCC TGCTTCCTAC GGAGCACAAC ATCCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgap35 ( XM_004916953.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004917010.1 Xenopus tropicalis Rho GTPase activating protein 35 (arhgap35), transcript variant X3, mRNA.

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CloneID	OXa14506
Clone ID Related Accession (Same CDS sequence)	XM_004916953.3 , XM_004916951.4 , XM_004916952.4 , XM_004916951.3 , XM_004916952.3 , XM_004916953.4 , XM_031891234.1
Accession Version	XM_004916953.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4419bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho GTPase-activating protein 35 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004916953.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGG CACGGAAACC AGATGTGCGT GTGCCCACCT ACAGTGTCAG CGTTGTGGGC 
CTTTCTGGCA CAGAGAAAGA GAAAGGCCAA TGTGGCGTTG GAAAATCATG CCTTTGTAAT 
CGATTTGTAA GACCCAGCGC AGATGATTTT CATCTGGACC ACACATCCGT CCTGAGCACT 
AGTGACTTTG GTGGTCGTGT TGTAAACAAT GACCATTTTC TGTACTGGGG AGAGAGTGGA 
CGGATGCCAG AAGACTGTGT AGACTTTAAA GTGCATGTGG TGGAGCAGAC AGAATTTATT 
GATGATCAGA CATTCCAGCC TCACCGTAGT ACTGCCCTAC AGCCTTACAT CAAGCGTGCT 
GCCTCCTGTA AGCTGGCCTC TGCTGAAAAA CTTATGTACT TCTGCACAGA TCAGTTGGGC 
TTGGAACAGG ACTTTGAGCA GAAGCAGATG CCTGAGGGTA AATTGTTGAT TGATGGTTTC 
CTGCTGTGTA TAGATGTTAG TCGTGGAATG AACCGGAACT TTGATGATCA GCTAAAATTT 
GTGTCCAATC TTTACACCCA GCTGGCCAAG ACAAAGAAAC CCATTGTTCT GGTTCTTACA 
AAATGTGATG AAGGAGTAGA ACGTTATATC CGCGATGCTC ATAGTTTTGC CCTTAACAAG 
AAGAACCTGC AAGTTGTAGA GACTTCTGCC CGGTCTAATG TGAATGTGGA TCTGGCCTTC 
ACTACACTAG TACAGCTTAT TGACAAAAGC CGTGGAAAGT CTAAAATTAT TCCTTATTTT 
GAGGCACTAA AGCAGCAGAG CCAACTGATT GCTGCCGCAA AGGACAAATA TGAATGGCTT 
GTGAGCCGAG TAGTAAAGTC TCATAATGAG ACATGGATCA ATCAAAGCAG GCGCATGCAG 
AGCAACATTG AATACCAAGA TTATGTGCAT TTGGAAGGAA CAGACAAAGC TAAGAAACTA 
TTCCATCAGC ACGTGCTACG GCTTAAACAG GAACACATAG AACGACGCAG AAGAAATTAC 
CTCTTAGCTC TCCCACGAGC CTTAGAAAGC CTTGTGCCAG ATCTGGATGA AATAGATCAC 
TTAAGCTGGG TTAAGGTTTC TAATTTGCTG GAATCAAAGG CAGAGTTCCT AAAATTTTTT 
ATTGTCCTGG AAGAGACACC TTGGGACTTC ACAATCCACA TTGACAGCAT TGATGAACGC 
ATACCTTATG ATTTGCTGGA AACAGTGCCT GCGGAAGAGG TCTATGCTGC CCACTTGGTC 
AAGTTAAGGA ATGCCAGGAA AAAGGAAGAA ATGAAGAGAT CTTTCAAGGA GAACTTGATC 
GCTTCTCCTT TCATTACACC AGGTAAGCCT TGGGAAGAGG CTCGGAGTTT TATCATGAGT 
GAAGAATTTT ACCAGTGGCT GGAAGAGCCT GTATATATGG AAATCTACAG CCGACACCAA 
AAAGAGATCA TTGATAAAGC TAAAGAGGAA TTTCAGGAGT TGTTGCTTGA GTATTCAGAG 
CTGTTTTATG AGCTAGAGTT AGATGCCAAA CCCAGTAAAG AGAAAATGGG CGTCATCCAG 
GATGTACTTG GTGAGGAACA ACGTTTCAAA GCATTACAGA AGCTGCAGGC GGAGCGAGAT 
GCCCTGATTC TTAAACACAT TCACTTTGTC TATCATCCAA CCAAGGACAC TTGTCCTAGC 
AACCCTTACT GTGTTGATAC AAGGATTGAG CATATACTCA GTTCCAGGTG CACATATCCC 
TATGACAGAC TACACAAAGA TCCAAATGTA GATAGGATCA ACCTTGTGAT CCTTGGAAAG 
GATGGTTTGG CACGAGAGTT AGCAAATGAG ATCCGAGCCC TCTGTACAAA TGATGATAAA 
TACGTAATTG ATGGAAAGAT GTATGAGTTA TCCCTAAGGC CTATAGAAGG CAATGTGCGG 
CTTCCTGTTA ATTCATTCCA GACATCCACT TTCCTCCCTC ATGGCTGTCT TTGCCTGTAC 
AACTCCAAGG AGTCTCTGTC CTATGTTGTG GAGAGCATTG AGAAAAGCAG AGAATCAAGC 
CTAGGTCGAA AGGAAAATCA CTTGATCCAT TTGCCTCTTA CGTTGATTCT TGTCAACAGG 
AGAGGAGACA CAAGTAGTGA AACTGCACAT AGCCTCATTC AACAGGGCAA ACAGGTTGCC 
AGTAAGCTTC AGTGTTTCTT TCTTGATGCA GCTTCCACTG GAATGGGATA TGGTCGCAAT 
ATCAATGAGA AGCAAATAAG CCTAATCCTC AGAGGGCTAC TGGAGTCAAA AAGAAACTTA 
AACCTTGTCA GCTCCACTCC AAGCATTAAA GATTTGACAG ATTGTGAGCT GCGTATTGTA 
ATGTGCCTCA TGTGTGCAGA CATATTTAAC ATAGAAGATG TGCTGAATGC ACTAAAACCT 
CACACTTACC GTCCATCACA ATGTGGGCTT GGTGGATCAG TTCTACTTGA CCTACCAATT 
GGCAGTCAAA AACGTCGCTT GGAGCTGCTG TTGTTGTCGT ATCATGCCTC CTTTAATGTT 
CGCAAGACTC GTTTAGTGCA CGGCTATATG ATTTTTTACG CAGCTAAGCG CCGTGCTTCC 
CTAGCTATGC TGCGTGCCTT TTTGTGTGAA GTGCAGGACA TTGTCCCTGT GCAGCTTGTT 
GCCTTGACTG AGGGACCTCT AACATTTTTG GATTATGTGG CACGAGAACA GTTACAAGAA 
GGGGAGGAAA TAGCTCAGGA CATTGAAGGT AAATTCCTTA TTCTACCACA AGGGCAGATA 
CAGCCAAAGT TGGATGTGTT TTACCCCTAC TTCAAAGATG TATTGGAGAA AAAAGTAATT 
ATTGAGGCCT CTCACATGTA TGATAATACA GCAGAGGCCT GCAGCACCAC AGAGGAAGTG 
TTTAGCTCAC CCCGTGCAGG CTCACCCCTT TGTAATTCCC ACTTGCCAGA CTCTGAAGAA 
GACCCTGAAC CTTCACCTCA ACACATTTTA TTTCAAAATG AACCTAACAT AGCAGCTCCC 
ATAAAATTTA CCAGAGAACT GGAGGAGACT GTGATGAACA CTTTAGAGAG CAAACTTAAC 
AACAAGGTAC CTCCTCCCCT TAAACCAAAG CCCCCTATTC CATATGAAAT TACCAAGCCT 
GAGCTATCAT ATTTGGATAC AGGGGCCCGG GATGGGCACC GTAAGTCCTT GACTTCCATA 
AACTGGCCCC CTTTGGAGGC CTTTGACCCT TCTGACTATG CTGAACCTAT GGATTCAGTA 
GTGAAGCCAA GAAACCAAGA GGAGAACATT TACTCTGTAC CCCATGATAG CACCCAAGGA 
AAGATCATCA CTATACGTAA TACCAACAAA CCACAATCTA ATGGGAGTGG CAATGGCTCA 
GACAGTGATA TGGACACAAA CTCATTGGAA CGAGCAAGAA AAGCATCCAT TGTCACTAAA 
CCTGTCCTTT ACCGTACCAA ATGTGCTAAA CTTGGGAAAT TCTCTAGCTA CAGGAGTAGT 
TTAAGTGTTG GCAGTGATGA TGAACCTGGA CCAATTCGAA GAAAGGAAGA GGAGACTGGA 
ACACAGGGTA CAAAGGGGGA CAGTACTACG AATTCTTATG AAGCTGATGG AGAGGACCCA 
CGCAAGAGGA ATATTTTGCG CAGTCTGCGA AGGACCACTA AGAAAGTGAA ACCCAAACCT 
CGTCCATCTT TAACCAAGGC GACCTGGGAG AGTAATTACT TTGGTTTCCC TCTGAGTAGT 
GTGGTGACTT CTGAACGACC AATTCCAGTA TTCATTGAGA AGTGTGTAGA GTACATCGAA 
GCCACGGGTG AGGGAATGAC CACAGAAGGC ATATACCGTG TTAGCGGGAA CAAATCTGAG 
ATGGAGTCTC TTCAAAGGCA ATTTGACCAA GATCATAACC TGGACCTGGC AGAAAAGGAT 
TTTACAGTGA ACACTGTGGC TGGAGCCTTG AAAAGTTTCT TCTCTGAATT ACCGGACCCT 
CTGGTGCCAT ACAATATGCA GGCTGAGCTG GTGGAGGCAT ATAAAATAAA TGATCTAGAA 
CATAAGCTCC AAGCTATGAA GGATCTTCTA AAAAAATTTC CAAAGGAAAA TCATGAAATC 
TTCAAATATG TTATATCTCA TTTAAACCGG GTTAGCCAGC ATCATCATAT AAATCTGATG 
ACCAGTGAAA ATTTATCTAT CTGCTTCTGG CCTACACTGA TGCGCCCTGA TTTTACCACT 
ATGGACGCTC TGACTGCAAC TCGCACATAC CAGACAATCA TTGAGCTTTT CATCCAACAG 
TGCCCCTTTT TCTTCTACCA GCGCCTTCCT GTGGACCTGC CTATCCCTAG TTCCCCATCT 
ACTCCACCTC TCACCCAGCC ATCTCCTCCT CAGTCCCCTC CTCTGACGCC TGTATCCCCC 
TCGCAGACCC TGCTTCCTAC GGAGCACAAC ATCCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004917010.1
CDS	280..4698
Translation	MMMARKPDVRVPTYSVSVVGLSGTEKEKGQCGVGKSCLCNRFVRPSADDFHLDHTSVLSTSDFGGRVVNNDHFLYWGESGRMPEDCVDFKVHVVEQTEFIDDQTFQPHRSTALQPYIKRAASCKLASAEKLMYFCTDQLGLEQDFEQKQMPEGKLLIDGFLLCIDVSRGMNRNFDDQLKFVSNLYTQLAKTKKPIVLVLTKCDEGVERYIRDAHSFALNKKNLQVVETSARSNVNVDLAFTTLVQLIDKSRGKSKIIPYFEALKQQSQLIAAAKDKYEWLVSRVVKSHNETWINQSRRMQSNIEYQDYVHLEGTDKAKKLFHQHVLRLKQEHIERRRRNYLLALPRALESLVPDLDEIDHLSWVKVSNLLESKAEFLKFFIVLEETPWDFTIHIDSIDERIPYDLLETVPAEEVYAAHLVKLRNARKKEEMKRSFKENLIASPFITPGKPWEEARSFIMSEEFYQWLEEPVYMEIYSRHQKEIIDKAKEEFQELLLEYSELFYELELDAKPSKEKMGVIQDVLGEEQRFKALQKLQAERDALILKHIHFVYHPTKDTCPSNPYCVDTRIEHILSSRCTYPYDRLHKDPNVDRINLVILGKDGLARELANEIRALCTNDDKYVIDGKMYELSLRPIEGNVRLPVNSFQTSTFLPHGCLCLYNSKESLSYVVESIEKSRESSLGRKENHLIHLPLTLILVNRRGDTSSETAHSLIQQGKQVASKLQCFFLDAASTGMGYGRNINEKQISLILRGLLESKRNLNLVSSTPSIKDLTDCELRIVMCLMCADIFNIEDVLNALKPHTYRPSQCGLGGSVLLDLPIGSQKRRLELLLLSYHASFNVRKTRLVHGYMIFYAAKRRASLAMLRAFLCEVQDIVPVQLVALTEGPLTFLDYVAREQLQEGEEIAQDIEGKFLILPQGQIQPKLDVFYPYFKDVLEKKVIIEASHMYDNTAEACSTTEEVFSSPRAGSPLCNSHLPDSEEDPEPSPQHILFQNEPNIAAPIKFTRELEETVMNTLESKLNNKVPPPLKPKPPIPYEITKPELSYLDTGARDGHRKSLTSINWPPLEAFDPSDYAEPMDSVVKPRNQEENIYSVPHDSTQGKIITIRNTNKPQSNGSGNGSDSDMDTNSLERARKASIVTKPVLYRTKCAKLGKFSSYRSSLSVGSDDEPGPIRRKEEETGTQGTKGDSTTNSYEADGEDPRKRNILRSLRRTTKKVKPKPRPSLTKATWESNYFGFPLSSVVTSERPIPVFIEKCVEYIEATGEGMTTEGIYRVSGNKSEMESLQRQFDQDHNLDLAEKDFTVNTVAGALKSFFSELPDPLVPYNMQAELVEAYKINDLEHKLQAMKDLLKKFPKENHEIFKYVISHLNRVSQHHHINLMTSENLSICFWPTLMRPDFTTMDALTATRTYQTIIELFIQQCPFFFYQRLPVDLPIPSSPSTPPLTQPSPPQSPPLTPVSPSQTLLPTEHNIL

Target ORF information:

RefSeq Version	XM_004916953.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho GTPase activating protein 35 (arhgap35), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004916953.4

ORF Insert Sequence:

ATGATGATGG CACGGAAACC AGATGTGCGT GTGCCCACCT ACAGTGTCAG CGTTGTGGGC 
CTTTCTGGCA CAGAGAAAGA GAAAGGCCAA TGTGGCGTTG GAAAATCATG CCTTTGTAAT 
CGATTTGTAA GACCCAGCGC AGATGATTTT CATCTGGACC ACACATCCGT CCTGAGCACT 
AGTGACTTTG GTGGTCGTGT TGTAAACAAT GACCATTTTC TGTACTGGGG AGAGAGTGGA 
CGGATGCCAG AAGACTGTGT AGACTTTAAA GTGCATGTGG TGGAGCAGAC AGAATTTATT 
GATGATCAGA CATTCCAGCC TCACCGTAGT ACTGCCCTAC AGCCTTACAT CAAGCGTGCT 
GCCTCCTGTA AGCTGGCCTC TGCTGAAAAA CTTATGTACT TCTGCACAGA TCAGTTGGGC 
TTGGAACAGG ACTTTGAGCA GAAGCAGATG CCTGAGGGTA AATTGTTGAT TGATGGTTTC 
CTGCTGTGTA TAGATGTTAG TCGTGGAATG AACCGGAACT TTGATGATCA GCTAAAATTT 
GTGTCCAATC TTTACACCCA GCTGGCCAAG ACAAAGAAAC CCATTGTTCT GGTTCTTACA 
AAATGTGATG AAGGAGTAGA ACGTTATATC CGCGATGCTC ATAGTTTTGC CCTTAACAAG 
AAGAACCTGC AAGTTGTAGA GACTTCTGCC CGGTCTAATG TGAATGTGGA TCTGGCCTTC 
ACTACACTAG TACAGCTTAT TGACAAAAGC CGTGGAAAGT CTAAAATTAT TCCTTATTTT 
GAGGCACTAA AGCAGCAGAG CCAACTGATT GCTGCCGCAA AGGACAAATA TGAATGGCTT 
GTGAGCCGAG TAGTAAAGTC TCATAATGAG ACATGGATCA ATCAAAGCAG GCGCATGCAG 
AGCAACATTG AATACCAAGA TTATGTGCAT TTGGAAGGAA CAGACAAAGC TAAGAAACTA 
TTCCATCAGC ACGTGCTACG GCTTAAACAG GAACACATAG AACGACGCAG AAGAAATTAC 
CTCTTAGCTC TCCCACGAGC CTTAGAAAGC CTTGTGCCAG ATCTGGATGA AATAGATCAC 
TTAAGCTGGG TTAAGGTTTC TAATTTGCTG GAATCAAAGG CAGAGTTCCT AAAATTTTTT 
ATTGTCCTGG AAGAGACACC TTGGGACTTC ACAATCCACA TTGACAGCAT TGATGAACGC 
ATACCTTATG ATTTGCTGGA AACAGTGCCT GCGGAAGAGG TCTATGCTGC CCACTTGGTC 
AAGTTAAGGA ATGCCAGGAA AAAGGAAGAA ATGAAGAGAT CTTTCAAGGA GAACTTGATC 
GCTTCTCCTT TCATTACACC AGGTAAGCCT TGGGAAGAGG CTCGGAGTTT TATCATGAGT 
GAAGAATTTT ACCAGTGGCT GGAAGAGCCT GTATATATGG AAATCTACAG CCGACACCAA 
AAAGAGATCA TTGATAAAGC TAAAGAGGAA TTTCAGGAGT TGTTGCTTGA GTATTCAGAG 
CTGTTTTATG AGCTAGAGTT AGATGCCAAA CCCAGTAAAG AGAAAATGGG CGTCATCCAG 
GATGTACTTG GTGAGGAACA ACGTTTCAAA GCATTACAGA AGCTGCAGGC GGAGCGAGAT 
GCCCTGATTC TTAAACACAT TCACTTTGTC TATCATCCAA CCAAGGACAC TTGTCCTAGC 
AACCCTTACT GTGTTGATAC AAGGATTGAG CATATACTCA GTTCCAGGTG CACATATCCC 
TATGACAGAC TACACAAAGA TCCAAATGTA GATAGGATCA ACCTTGTGAT CCTTGGAAAG 
GATGGTTTGG CACGAGAGTT AGCAAATGAG ATCCGAGCCC TCTGTACAAA TGATGATAAA 
TACGTAATTG ATGGAAAGAT GTATGAGTTA TCCCTAAGGC CTATAGAAGG CAATGTGCGG 
CTTCCTGTTA ATTCATTCCA GACATCCACT TTCCTCCCTC ATGGCTGTCT TTGCCTGTAC 
AACTCCAAGG AGTCTCTGTC CTATGTTGTG GAGAGCATTG AGAAAAGCAG AGAATCAAGC 
CTAGGTCGAA AGGAAAATCA CTTGATCCAT TTGCCTCTTA CGTTGATTCT TGTCAACAGG 
AGAGGAGACA CAAGTAGTGA AACTGCACAT AGCCTCATTC AACAGGGCAA ACAGGTTGCC 
AGTAAGCTTC AGTGTTTCTT TCTTGATGCA GCTTCCACTG GAATGGGATA TGGTCGCAAT 
ATCAATGAGA AGCAAATAAG CCTAATCCTC AGAGGGCTAC TGGAGTCAAA AAGAAACTTA 
AACCTTGTCA GCTCCACTCC AAGCATTAAA GATTTGACAG ATTGTGAGCT GCGTATTGTA 
ATGTGCCTCA TGTGTGCAGA CATATTTAAC ATAGAAGATG TGCTGAATGC ACTAAAACCT 
CACACTTACC GTCCATCACA ATGTGGGCTT GGTGGATCAG TTCTACTTGA CCTACCAATT 
GGCAGTCAAA AACGTCGCTT GGAGCTGCTG TTGTTGTCGT ATCATGCCTC CTTTAATGTT 
CGCAAGACTC GTTTAGTGCA CGGCTATATG ATTTTTTACG CAGCTAAGCG CCGTGCTTCC 
CTAGCTATGC TGCGTGCCTT TTTGTGTGAA GTGCAGGACA TTGTCCCTGT GCAGCTTGTT 
GCCTTGACTG AGGGACCTCT AACATTTTTG GATTATGTGG CACGAGAACA GTTACAAGAA 
GGGGAGGAAA TAGCTCAGGA CATTGAAGGT AAATTCCTTA TTCTACCACA AGGGCAGATA 
CAGCCAAAGT TGGATGTGTT TTACCCCTAC TTCAAAGATG TATTGGAGAA AAAAGTAATT 
ATTGAGGCCT CTCACATGTA TGATAATACA GCAGAGGCCT GCAGCACCAC AGAGGAAGTG 
TTTAGCTCAC CCCGTGCAGG CTCACCCCTT TGTAATTCCC ACTTGCCAGA CTCTGAAGAA 
GACCCTGAAC CTTCACCTCA ACACATTTTA TTTCAAAATG AACCTAACAT AGCAGCTCCC 
ATAAAATTTA CCAGAGAACT GGAGGAGACT GTGATGAACA CTTTAGAGAG CAAACTTAAC 
AACAAGGTAC CTCCTCCCCT TAAACCAAAG CCCCCTATTC CATATGAAAT TACCAAGCCT 
GAGCTATCAT ATTTGGATAC AGGGGCCCGG GATGGGCACC GTAAGTCCTT GACTTCCATA 
AACTGGCCCC CTTTGGAGGC CTTTGACCCT TCTGACTATG CTGAACCTAT GGATTCAGTA 
GTGAAGCCAA GAAACCAAGA GGAGAACATT TACTCTGTAC CCCATGATAG CACCCAAGGA 
AAGATCATCA CTATACGTAA TACCAACAAA CCACAATCTA ATGGGAGTGG CAATGGCTCA 
GACAGTGATA TGGACACAAA CTCATTGGAA CGAGCAAGAA AAGCATCCAT TGTCACTAAA 
CCTGTCCTTT ACCGTACCAA ATGTGCTAAA CTTGGGAAAT TCTCTAGCTA CAGGAGTAGT 
TTAAGTGTTG GCAGTGATGA TGAACCTGGA CCAATTCGAA GAAAGGAAGA GGAGACTGGA 
ACACAGGGTA CAAAGGGGGA CAGTACTACG AATTCTTATG AAGCTGATGG AGAGGACCCA 
CGCAAGAGGA ATATTTTGCG CAGTCTGCGA AGGACCACTA AGAAAGTGAA ACCCAAACCT 
CGTCCATCTT TAACCAAGGC GACCTGGGAG AGTAATTACT TTGGTTTCCC TCTGAGTAGT 
GTGGTGACTT CTGAACGACC AATTCCAGTA TTCATTGAGA AGTGTGTAGA GTACATCGAA 
GCCACGGGTG AGGGAATGAC CACAGAAGGC ATATACCGTG TTAGCGGGAA CAAATCTGAG 
ATGGAGTCTC TTCAAAGGCA ATTTGACCAA GATCATAACC TGGACCTGGC AGAAAAGGAT 
TTTACAGTGA ACACTGTGGC TGGAGCCTTG AAAAGTTTCT TCTCTGAATT ACCGGACCCT 
CTGGTGCCAT ACAATATGCA GGCTGAGCTG GTGGAGGCAT ATAAAATAAA TGATCTAGAA 
CATAAGCTCC AAGCTATGAA GGATCTTCTA AAAAAATTTC CAAAGGAAAA TCATGAAATC 
TTCAAATATG TTATATCTCA TTTAAACCGG GTTAGCCAGC ATCATCATAT AAATCTGATG 
ACCAGTGAAA ATTTATCTAT CTGCTTCTGG CCTACACTGA TGCGCCCTGA TTTTACCACT 
ATGGACGCTC TGACTGCAAC TCGCACATAC CAGACAATCA TTGAGCTTTT CATCCAACAG 
TGCCCCTTTT TCTTCTACCA GCGCCTTCCT GTGGACCTGC CTATCCCTAG TTCCCCATCT 
ACTCCACCTC TCACCCAGCC ATCTCCTCCT CAGTCCCCTC CTCTGACGCC TGTATCCCCC 
TCGCAGACCC TGCTTCCTAC GGAGCACAAC ATCCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgap35 ( XM_031891234.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031747094.1 Xenopus tropicalis Rho GTPase activating protein 35 (arhgap35), transcript variant X4, mRNA.

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CloneID	OXa14506
Clone ID Related Accession (Same CDS sequence)	XM_004916953.3 , XM_004916951.4 , XM_004916952.4 , XM_004916951.3 , XM_004916952.3 , XM_004916953.4 , XM_031891234.1
Accession Version	XM_031891234.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4419bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho GTPase-activating protein 35 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGG CACGGAAACC AGATGTGCGT GTGCCCACCT ACAGTGTCAG CGTTGTGGGC 
CTTTCTGGCA CAGAGAAAGA GAAAGGCCAA TGTGGCGTTG GAAAATCATG CCTTTGTAAT 
CGATTTGTAA GACCCAGCGC AGATGATTTT CATCTGGACC ACACATCCGT CCTGAGCACT 
AGTGACTTTG GTGGTCGTGT TGTAAACAAT GACCATTTTC TGTACTGGGG AGAGAGTGGA 
CGGATGCCAG AAGACTGTGT AGACTTTAAA GTGCATGTGG TGGAGCAGAC AGAATTTATT 
GATGATCAGA CATTCCAGCC TCACCGTAGT ACTGCCCTAC AGCCTTACAT CAAGCGTGCT 
GCCTCCTGTA AGCTGGCCTC TGCTGAAAAA CTTATGTACT TCTGCACAGA TCAGTTGGGC 
TTGGAACAGG ACTTTGAGCA GAAGCAGATG CCTGAGGGTA AATTGTTGAT TGATGGTTTC 
CTGCTGTGTA TAGATGTTAG TCGTGGAATG AACCGGAACT TTGATGATCA GCTAAAATTT 
GTGTCCAATC TTTACACCCA GCTGGCCAAG ACAAAGAAAC CCATTGTTCT GGTTCTTACA 
AAATGTGATG AAGGAGTAGA ACGTTATATC CGCGATGCTC ATAGTTTTGC CCTTAACAAG 
AAGAACCTGC AAGTTGTAGA GACTTCTGCC CGGTCTAATG TGAATGTGGA TCTGGCCTTC 
ACTACACTAG TACAGCTTAT TGACAAAAGC CGTGGAAAGT CTAAAATTAT TCCTTATTTT 
GAGGCACTAA AGCAGCAGAG CCAACTGATT GCTGCCGCAA AGGACAAATA TGAATGGCTT 
GTGAGCCGAG TAGTAAAGTC TCATAATGAG ACATGGATCA ATCAAAGCAG GCGCATGCAG 
AGCAACATTG AATACCAAGA TTATGTGCAT TTGGAAGGAA CAGACAAAGC TAAGAAACTA 
TTCCATCAGC ACGTGCTACG GCTTAAACAG GAACACATAG AACGACGCAG AAGAAATTAC 
CTCTTAGCTC TCCCACGAGC CTTAGAAAGC CTTGTGCCAG ATCTGGATGA AATAGATCAC 
TTAAGCTGGG TTAAGGTTTC TAATTTGCTG GAATCAAAGG CAGAGTTCCT AAAATTTTTT 
ATTGTCCTGG AAGAGACACC TTGGGACTTC ACAATCCACA TTGACAGCAT TGATGAACGC 
ATACCTTATG ATTTGCTGGA AACAGTGCCT GCGGAAGAGG TCTATGCTGC CCACTTGGTC 
AAGTTAAGGA ATGCCAGGAA AAAGGAAGAA ATGAAGAGAT CTTTCAAGGA GAACTTGATC 
GCTTCTCCTT TCATTACACC AGGTAAGCCT TGGGAAGAGG CTCGGAGTTT TATCATGAGT 
GAAGAATTTT ACCAGTGGCT GGAAGAGCCT GTATATATGG AAATCTACAG CCGACACCAA 
AAAGAGATCA TTGATAAAGC TAAAGAGGAA TTTCAGGAGT TGTTGCTTGA GTATTCAGAG 
CTGTTTTATG AGCTAGAGTT AGATGCCAAA CCCAGTAAAG AGAAAATGGG CGTCATCCAG 
GATGTACTTG GTGAGGAACA ACGTTTCAAA GCATTACAGA AGCTGCAGGC GGAGCGAGAT 
GCCCTGATTC TTAAACACAT TCACTTTGTC TATCATCCAA CCAAGGACAC TTGTCCTAGC 
AACCCTTACT GTGTTGATAC AAGGATTGAG CATATACTCA GTTCCAGGTG CACATATCCC 
TATGACAGAC TACACAAAGA TCCAAATGTA GATAGGATCA ACCTTGTGAT CCTTGGAAAG 
GATGGTTTGG CACGAGAGTT AGCAAATGAG ATCCGAGCCC TCTGTACAAA TGATGATAAA 
TACGTAATTG ATGGAAAGAT GTATGAGTTA TCCCTAAGGC CTATAGAAGG CAATGTGCGG 
CTTCCTGTTA ATTCATTCCA GACATCCACT TTCCTCCCTC ATGGCTGTCT TTGCCTGTAC 
AACTCCAAGG AGTCTCTGTC CTATGTTGTG GAGAGCATTG AGAAAAGCAG AGAATCAAGC 
CTAGGTCGAA AGGAAAATCA CTTGATCCAT TTGCCTCTTA CGTTGATTCT TGTCAACAGG 
AGAGGAGACA CAAGTAGTGA AACTGCACAT AGCCTCATTC AACAGGGCAA ACAGGTTGCC 
AGTAAGCTTC AGTGTTTCTT TCTTGATGCA GCTTCCACTG GAATGGGATA TGGTCGCAAT 
ATCAATGAGA AGCAAATAAG CCTAATCCTC AGAGGGCTAC TGGAGTCAAA AAGAAACTTA 
AACCTTGTCA GCTCCACTCC AAGCATTAAA GATTTGACAG ATTGTGAGCT GCGTATTGTA 
ATGTGCCTCA TGTGTGCAGA CATATTTAAC ATAGAAGATG TGCTGAATGC ACTAAAACCT 
CACACTTACC GTCCATCACA ATGTGGGCTT GGTGGATCAG TTCTACTTGA CCTACCAATT 
GGCAGTCAAA AACGTCGCTT GGAGCTGCTG TTGTTGTCGT ATCATGCCTC CTTTAATGTT 
CGCAAGACTC GTTTAGTGCA CGGCTATATG ATTTTTTACG CAGCTAAGCG CCGTGCTTCC 
CTAGCTATGC TGCGTGCCTT TTTGTGTGAA GTGCAGGACA TTGTCCCTGT GCAGCTTGTT 
GCCTTGACTG AGGGACCTCT AACATTTTTG GATTATGTGG CACGAGAACA GTTACAAGAA 
GGGGAGGAAA TAGCTCAGGA CATTGAAGGT AAATTCCTTA TTCTACCACA AGGGCAGATA 
CAGCCAAAGT TGGATGTGTT TTACCCCTAC TTCAAAGATG TATTGGAGAA AAAAGTAATT 
ATTGAGGCCT CTCACATGTA TGATAATACA GCAGAGGCCT GCAGCACCAC AGAGGAAGTG 
TTTAGCTCAC CCCGTGCAGG CTCACCCCTT TGTAATTCCC ACTTGCCAGA CTCTGAAGAA 
GACCCTGAAC CTTCACCTCA ACACATTTTA TTTCAAAATG AACCTAACAT AGCAGCTCCC 
ATAAAATTTA CCAGAGAACT GGAGGAGACT GTGATGAACA CTTTAGAGAG CAAACTTAAC 
AACAAGGTAC CTCCTCCCCT TAAACCAAAG CCCCCTATTC CATATGAAAT TACCAAGCCT 
GAGCTATCAT ATTTGGATAC AGGGGCCCGG GATGGGCACC GTAAGTCCTT GACTTCCATA 
AACTGGCCCC CTTTGGAGGC CTTTGACCCT TCTGACTATG CTGAACCTAT GGATTCAGTA 
GTGAAGCCAA GAAACCAAGA GGAGAACATT TACTCTGTAC CCCATGATAG CACCCAAGGA 
AAGATCATCA CTATACGTAA TACCAACAAA CCACAATCTA ATGGGAGTGG CAATGGCTCA 
GACAGTGATA TGGACACAAA CTCATTGGAA CGAGCAAGAA AAGCATCCAT TGTCACTAAA 
CCTGTCCTTT ACCGTACCAA ATGTGCTAAA CTTGGGAAAT TCTCTAGCTA CAGGAGTAGT 
TTAAGTGTTG GCAGTGATGA TGAACCTGGA CCAATTCGAA GAAAGGAAGA GGAGACTGGA 
ACACAGGGTA CAAAGGGGGA CAGTACTACG AATTCTTATG AAGCTGATGG AGAGGACCCA 
CGCAAGAGGA ATATTTTGCG CAGTCTGCGA AGGACCACTA AGAAAGTGAA ACCCAAACCT 
CGTCCATCTT TAACCAAGGC GACCTGGGAG AGTAATTACT TTGGTTTCCC TCTGAGTAGT 
GTGGTGACTT CTGAACGACC AATTCCAGTA TTCATTGAGA AGTGTGTAGA GTACATCGAA 
GCCACGGGTG AGGGAATGAC CACAGAAGGC ATATACCGTG TTAGCGGGAA CAAATCTGAG 
ATGGAGTCTC TTCAAAGGCA ATTTGACCAA GATCATAACC TGGACCTGGC AGAAAAGGAT 
TTTACAGTGA ACACTGTGGC TGGAGCCTTG AAAAGTTTCT TCTCTGAATT ACCGGACCCT 
CTGGTGCCAT ACAATATGCA GGCTGAGCTG GTGGAGGCAT ATAAAATAAA TGATCTAGAA 
CATAAGCTCC AAGCTATGAA GGATCTTCTA AAAAAATTTC CAAAGGAAAA TCATGAAATC 
TTCAAATATG TTATATCTCA TTTAAACCGG GTTAGCCAGC ATCATCATAT AAATCTGATG 
ACCAGTGAAA ATTTATCTAT CTGCTTCTGG CCTACACTGA TGCGCCCTGA TTTTACCACT 
ATGGACGCTC TGACTGCAAC TCGCACATAC CAGACAATCA TTGAGCTTTT CATCCAACAG 
TGCCCCTTTT TCTTCTACCA GCGCCTTCCT GTGGACCTGC CTATCCCTAG TTCCCCATCT 
ACTCCACCTC TCACCCAGCC ATCTCCTCCT CAGTCCCCTC CTCTGACGCC TGTATCCCCC 
TCGCAGACCC TGCTTCCTAC GGAGCACAAC ATCCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031747094.1
CDS	607..5025
Translation	MMMARKPDVRVPTYSVSVVGLSGTEKEKGQCGVGKSCLCNRFVRPSADDFHLDHTSVLSTSDFGGRVVNNDHFLYWGESGRMPEDCVDFKVHVVEQTEFIDDQTFQPHRSTALQPYIKRAASCKLASAEKLMYFCTDQLGLEQDFEQKQMPEGKLLIDGFLLCIDVSRGMNRNFDDQLKFVSNLYTQLAKTKKPIVLVLTKCDEGVERYIRDAHSFALNKKNLQVVETSARSNVNVDLAFTTLVQLIDKSRGKSKIIPYFEALKQQSQLIAAAKDKYEWLVSRVVKSHNETWINQSRRMQSNIEYQDYVHLEGTDKAKKLFHQHVLRLKQEHIERRRRNYLLALPRALESLVPDLDEIDHLSWVKVSNLLESKAEFLKFFIVLEETPWDFTIHIDSIDERIPYDLLETVPAEEVYAAHLVKLRNARKKEEMKRSFKENLIASPFITPGKPWEEARSFIMSEEFYQWLEEPVYMEIYSRHQKEIIDKAKEEFQELLLEYSELFYELELDAKPSKEKMGVIQDVLGEEQRFKALQKLQAERDALILKHIHFVYHPTKDTCPSNPYCVDTRIEHILSSRCTYPYDRLHKDPNVDRINLVILGKDGLARELANEIRALCTNDDKYVIDGKMYELSLRPIEGNVRLPVNSFQTSTFLPHGCLCLYNSKESLSYVVESIEKSRESSLGRKENHLIHLPLTLILVNRRGDTSSETAHSLIQQGKQVASKLQCFFLDAASTGMGYGRNINEKQISLILRGLLESKRNLNLVSSTPSIKDLTDCELRIVMCLMCADIFNIEDVLNALKPHTYRPSQCGLGGSVLLDLPIGSQKRRLELLLLSYHASFNVRKTRLVHGYMIFYAAKRRASLAMLRAFLCEVQDIVPVQLVALTEGPLTFLDYVAREQLQEGEEIAQDIEGKFLILPQGQIQPKLDVFYPYFKDVLEKKVIIEASHMYDNTAEACSTTEEVFSSPRAGSPLCNSHLPDSEEDPEPSPQHILFQNEPNIAAPIKFTRELEETVMNTLESKLNNKVPPPLKPKPPIPYEITKPELSYLDTGARDGHRKSLTSINWPPLEAFDPSDYAEPMDSVVKPRNQEENIYSVPHDSTQGKIITIRNTNKPQSNGSGNGSDSDMDTNSLERARKASIVTKPVLYRTKCAKLGKFSSYRSSLSVGSDDEPGPIRRKEEETGTQGTKGDSTTNSYEADGEDPRKRNILRSLRRTTKKVKPKPRPSLTKATWESNYFGFPLSSVVTSERPIPVFIEKCVEYIEATGEGMTTEGIYRVSGNKSEMESLQRQFDQDHNLDLAEKDFTVNTVAGALKSFFSELPDPLVPYNMQAELVEAYKINDLEHKLQAMKDLLKKFPKENHEIFKYVISHLNRVSQHHHINLMTSENLSICFWPTLMRPDFTTMDALTATRTYQTIIELFIQQCPFFFYQRLPVDLPIPSSPSTPPLTQPSPPQSPPLTPVSPSQTLLPTEHNIL

Target ORF information:

RefSeq Version	XM_031891234.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho GTPase activating protein 35 (arhgap35), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031891234.1

ORF Insert Sequence:

ATGATGATGG CACGGAAACC AGATGTGCGT GTGCCCACCT ACAGTGTCAG CGTTGTGGGC 
CTTTCTGGCA CAGAGAAAGA GAAAGGCCAA TGTGGCGTTG GAAAATCATG CCTTTGTAAT 
CGATTTGTAA GACCCAGCGC AGATGATTTT CATCTGGACC ACACATCCGT CCTGAGCACT 
AGTGACTTTG GTGGTCGTGT TGTAAACAAT GACCATTTTC TGTACTGGGG AGAGAGTGGA 
CGGATGCCAG AAGACTGTGT AGACTTTAAA GTGCATGTGG TGGAGCAGAC AGAATTTATT 
GATGATCAGA CATTCCAGCC TCACCGTAGT ACTGCCCTAC AGCCTTACAT CAAGCGTGCT 
GCCTCCTGTA AGCTGGCCTC TGCTGAAAAA CTTATGTACT TCTGCACAGA TCAGTTGGGC 
TTGGAACAGG ACTTTGAGCA GAAGCAGATG CCTGAGGGTA AATTGTTGAT TGATGGTTTC 
CTGCTGTGTA TAGATGTTAG TCGTGGAATG AACCGGAACT TTGATGATCA GCTAAAATTT 
GTGTCCAATC TTTACACCCA GCTGGCCAAG ACAAAGAAAC CCATTGTTCT GGTTCTTACA 
AAATGTGATG AAGGAGTAGA ACGTTATATC CGCGATGCTC ATAGTTTTGC CCTTAACAAG 
AAGAACCTGC AAGTTGTAGA GACTTCTGCC CGGTCTAATG TGAATGTGGA TCTGGCCTTC 
ACTACACTAG TACAGCTTAT TGACAAAAGC CGTGGAAAGT CTAAAATTAT TCCTTATTTT 
GAGGCACTAA AGCAGCAGAG CCAACTGATT GCTGCCGCAA AGGACAAATA TGAATGGCTT 
GTGAGCCGAG TAGTAAAGTC TCATAATGAG ACATGGATCA ATCAAAGCAG GCGCATGCAG 
AGCAACATTG AATACCAAGA TTATGTGCAT TTGGAAGGAA CAGACAAAGC TAAGAAACTA 
TTCCATCAGC ACGTGCTACG GCTTAAACAG GAACACATAG AACGACGCAG AAGAAATTAC 
CTCTTAGCTC TCCCACGAGC CTTAGAAAGC CTTGTGCCAG ATCTGGATGA AATAGATCAC 
TTAAGCTGGG TTAAGGTTTC TAATTTGCTG GAATCAAAGG CAGAGTTCCT AAAATTTTTT 
ATTGTCCTGG AAGAGACACC TTGGGACTTC ACAATCCACA TTGACAGCAT TGATGAACGC 
ATACCTTATG ATTTGCTGGA AACAGTGCCT GCGGAAGAGG TCTATGCTGC CCACTTGGTC 
AAGTTAAGGA ATGCCAGGAA AAAGGAAGAA ATGAAGAGAT CTTTCAAGGA GAACTTGATC 
GCTTCTCCTT TCATTACACC AGGTAAGCCT TGGGAAGAGG CTCGGAGTTT TATCATGAGT 
GAAGAATTTT ACCAGTGGCT GGAAGAGCCT GTATATATGG AAATCTACAG CCGACACCAA 
AAAGAGATCA TTGATAAAGC TAAAGAGGAA TTTCAGGAGT TGTTGCTTGA GTATTCAGAG 
CTGTTTTATG AGCTAGAGTT AGATGCCAAA CCCAGTAAAG AGAAAATGGG CGTCATCCAG 
GATGTACTTG GTGAGGAACA ACGTTTCAAA GCATTACAGA AGCTGCAGGC GGAGCGAGAT 
GCCCTGATTC TTAAACACAT TCACTTTGTC TATCATCCAA CCAAGGACAC TTGTCCTAGC 
AACCCTTACT GTGTTGATAC AAGGATTGAG CATATACTCA GTTCCAGGTG CACATATCCC 
TATGACAGAC TACACAAAGA TCCAAATGTA GATAGGATCA ACCTTGTGAT CCTTGGAAAG 
GATGGTTTGG CACGAGAGTT AGCAAATGAG ATCCGAGCCC TCTGTACAAA TGATGATAAA 
TACGTAATTG ATGGAAAGAT GTATGAGTTA TCCCTAAGGC CTATAGAAGG CAATGTGCGG 
CTTCCTGTTA ATTCATTCCA GACATCCACT TTCCTCCCTC ATGGCTGTCT TTGCCTGTAC 
AACTCCAAGG AGTCTCTGTC CTATGTTGTG GAGAGCATTG AGAAAAGCAG AGAATCAAGC 
CTAGGTCGAA AGGAAAATCA CTTGATCCAT TTGCCTCTTA CGTTGATTCT TGTCAACAGG 
AGAGGAGACA CAAGTAGTGA AACTGCACAT AGCCTCATTC AACAGGGCAA ACAGGTTGCC 
AGTAAGCTTC AGTGTTTCTT TCTTGATGCA GCTTCCACTG GAATGGGATA TGGTCGCAAT 
ATCAATGAGA AGCAAATAAG CCTAATCCTC AGAGGGCTAC TGGAGTCAAA AAGAAACTTA 
AACCTTGTCA GCTCCACTCC AAGCATTAAA GATTTGACAG ATTGTGAGCT GCGTATTGTA 
ATGTGCCTCA TGTGTGCAGA CATATTTAAC ATAGAAGATG TGCTGAATGC ACTAAAACCT 
CACACTTACC GTCCATCACA ATGTGGGCTT GGTGGATCAG TTCTACTTGA CCTACCAATT 
GGCAGTCAAA AACGTCGCTT GGAGCTGCTG TTGTTGTCGT ATCATGCCTC CTTTAATGTT 
CGCAAGACTC GTTTAGTGCA CGGCTATATG ATTTTTTACG CAGCTAAGCG CCGTGCTTCC 
CTAGCTATGC TGCGTGCCTT TTTGTGTGAA GTGCAGGACA TTGTCCCTGT GCAGCTTGTT 
GCCTTGACTG AGGGACCTCT AACATTTTTG GATTATGTGG CACGAGAACA GTTACAAGAA 
GGGGAGGAAA TAGCTCAGGA CATTGAAGGT AAATTCCTTA TTCTACCACA AGGGCAGATA 
CAGCCAAAGT TGGATGTGTT TTACCCCTAC TTCAAAGATG TATTGGAGAA AAAAGTAATT 
ATTGAGGCCT CTCACATGTA TGATAATACA GCAGAGGCCT GCAGCACCAC AGAGGAAGTG 
TTTAGCTCAC CCCGTGCAGG CTCACCCCTT TGTAATTCCC ACTTGCCAGA CTCTGAAGAA 
GACCCTGAAC CTTCACCTCA ACACATTTTA TTTCAAAATG AACCTAACAT AGCAGCTCCC 
ATAAAATTTA CCAGAGAACT GGAGGAGACT GTGATGAACA CTTTAGAGAG CAAACTTAAC 
AACAAGGTAC CTCCTCCCCT TAAACCAAAG CCCCCTATTC CATATGAAAT TACCAAGCCT 
GAGCTATCAT ATTTGGATAC AGGGGCCCGG GATGGGCACC GTAAGTCCTT GACTTCCATA 
AACTGGCCCC CTTTGGAGGC CTTTGACCCT TCTGACTATG CTGAACCTAT GGATTCAGTA 
GTGAAGCCAA GAAACCAAGA GGAGAACATT TACTCTGTAC CCCATGATAG CACCCAAGGA 
AAGATCATCA CTATACGTAA TACCAACAAA CCACAATCTA ATGGGAGTGG CAATGGCTCA 
GACAGTGATA TGGACACAAA CTCATTGGAA CGAGCAAGAA AAGCATCCAT TGTCACTAAA 
CCTGTCCTTT ACCGTACCAA ATGTGCTAAA CTTGGGAAAT TCTCTAGCTA CAGGAGTAGT 
TTAAGTGTTG GCAGTGATGA TGAACCTGGA CCAATTCGAA GAAAGGAAGA GGAGACTGGA 
ACACAGGGTA CAAAGGGGGA CAGTACTACG AATTCTTATG AAGCTGATGG AGAGGACCCA 
CGCAAGAGGA ATATTTTGCG CAGTCTGCGA AGGACCACTA AGAAAGTGAA ACCCAAACCT 
CGTCCATCTT TAACCAAGGC GACCTGGGAG AGTAATTACT TTGGTTTCCC TCTGAGTAGT 
GTGGTGACTT CTGAACGACC AATTCCAGTA TTCATTGAGA AGTGTGTAGA GTACATCGAA 
GCCACGGGTG AGGGAATGAC CACAGAAGGC ATATACCGTG TTAGCGGGAA CAAATCTGAG 
ATGGAGTCTC TTCAAAGGCA ATTTGACCAA GATCATAACC TGGACCTGGC AGAAAAGGAT 
TTTACAGTGA ACACTGTGGC TGGAGCCTTG AAAAGTTTCT TCTCTGAATT ACCGGACCCT 
CTGGTGCCAT ACAATATGCA GGCTGAGCTG GTGGAGGCAT ATAAAATAAA TGATCTAGAA 
CATAAGCTCC AAGCTATGAA GGATCTTCTA AAAAAATTTC CAAAGGAAAA TCATGAAATC 
TTCAAATATG TTATATCTCA TTTAAACCGG GTTAGCCAGC ATCATCATAT AAATCTGATG 
ACCAGTGAAA ATTTATCTAT CTGCTTCTGG CCTACACTGA TGCGCCCTGA TTTTACCACT 
ATGGACGCTC TGACTGCAAC TCGCACATAC CAGACAATCA TTGAGCTTTT CATCCAACAG 
TGCCCCTTTT TCTTCTACCA GCGCCTTCCT GTGGACCTGC CTATCCCTAG TTCCCCATCT 
ACTCCACCTC TCACCCAGCC ATCTCCTCCT CAGTCCCCTC CTCTGACGCC TGTATCCCCC 
TCGCAGACCC TGCTTCCTAC GGAGCACAAC ATCCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgap35 ( XM_002932597.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002932643.1 Xenopus tropicalis Rho GTPase activating protein 35 (arhgap35), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OXa14507
Clone ID Related Accession (Same CDS sequence)	XM_002932597.4 , XM_002932597.5
Accession Version	XM_002932597.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4413bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho GTPase-activating protein 35 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002932597.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGG CACGGAAACC AGATGTGCGT GTGCCCACCT ACAGTGTCAG CGTTGTGGGC 
CTTTCTGGCA CAGAGAAAGA GAAAGGCCAA TGTGGCGTTG GAAAATCATG CCTTTGTAAT 
CGATTTGTAA GACCCAGCGC AGATGATTTT CATCTGGACC ACACATCCGT CCTGAGCACT 
AGTGACTTTG GTGGTCGTGT TGTAAACAAT GACCATTTTC TGTACTGGGG AGAGAGTGGA 
CGGATGCCAG AAGACTGTGT AGACTTTAAA GTGCATGTGG TGGAGCAGAC AGAATTTATT 
GATGATCAGA CATTCCAGCC TCACCGTAGT ACTGCCCTAC AGCCTTACAT CAAGCGTGCT 
GCCTCCTGTA AGCTGGCCTC TGCTGAAAAA CTTATGTACT TCTGCACAGA TCAGTTGGGC 
TTGGAACAGG ACTTTGAGCA GAAGCAGATG CCTGAGGGTA AATTGTTGAT TGATGGTTTC 
CTGCTGTGTA TAGATGTTAG TCGTGGAATG AACCGGAACT TTGATGATCA GCTAAAATTT 
GTGTCCAATC TTTACACCCA GCTGGCCAAG ACAAAGAAAC CCATTGTTCT GGTTCTTACA 
AAATGTGATG AAGGAGTAGA ACGTTATATC CGCGATGCTC ATAGTTTTGC CCTTAACAAG 
AAGAACCTGC AAGTTGTAGA GACTTCTGCC CGGTCTAATG TGAATGTGGA TCTGGCCTTC 
ACTACACTAG TACAGCTTAT TGACAAAAGC CGTGGAAAGT CTAAAATTAT TCCTTATTTT 
GAGGCACTAA AGCAGCAGAG CCAACTGATT GCTGCCGCAA AGGACAAATA TGAATGGCTT 
GTGAGCCGAG TAGTAAAGTC TCATAATGAG ACATGGATCA ATCAAAGCAG GCGCATGCAG 
AGCAACATTG AATACCAAGA TTATGTGCAT TTGGAAGGAA CAGACAAAGC TAAGAAACTA 
TTCCATCAGC ACGTGCTACG GCTTAAACAG GAACACATAG AACGACGCAG AAGAAATTAC 
CTCTTAGCTC TCCCACGAGC CTTAGAAAGC CTTGTGCCAG ATCTGGATGA AATAGATCAC 
TTAAGCTGGG TTAAGGTTTC TAATTTGCTG GAATCAAAGG CAGAGTTCCT AAAATTTTTT 
ATTGTCCTGG AAGAGACACC TTGGGACTTC ACAATCCACA TTGACAGCAT TGATGAACGC 
ATACCTTATG ATTTGCTGGA AACAGTGCCT GCGGAAGAGG TCTATGCTGC CCACTTGGTC 
AAGTTAAGGA ATGCCAGGAA AAAGGAAGAA ATGAAGAGAT CTTTCAAGGA GAACTTGATC 
GCTTCTCCTT TCATTACACC AGGTAAGCCT TGGGAAGAGG CTCGGAGTTT TATCATGAGT 
GAAGAATTTT ACCAGTGGCT GGAAGAGCCT GTATATATGG AAATCTACAG CCGACACCAA 
AAAGAGATCA TTGATAAAGC TAAAGAGGAA TTTCAGGAGT TGTTGCTTGA GTATTCAGAG 
CTGTTTTATG AGCTAGAGTT AGATGCCAAA CCCAGTAAAG AGAAAATGGG CGTCATCCAG 
GATGTACTTG GTGAGGAACA ACGTTTCAAA GCATTACAGA AGCTGCAGGC GGAGCGAGAT 
GCCCTGATTC TTAAACACAT TCACTTTGTC TATCATCCAA CCAAGGACAC TTGTCCTAGC 
AACCCTTACT GTGTTGATAC AAGGATTGAG CATATACTCA GTTCCAGGTG CACATATCCC 
TATGACAGAC TACACAAAGA TCCAAATGTA GATAGGATCA ACCTTGTGAT CCTTGGAAAG 
GATGGTTTGG CACGAGAGTT AGCAAATGAG ATCCGAGCCC TCTGTACAAA TGATGATAAA 
TACGTAATTG ATGGAAAGAT GTATGAGTTA TCCCTAAGGC CTATAGAAGG CAATGTGCGG 
CTTCCTGTTA ATTCATTCCA GACATCCACT TTCCTCCCTC ATGGCTGTCT TTGCCTGTAC 
AACTCCAAGG AGTCTCTGTC CTATGTTGTG GAGAGCATTG AGAAAAGCAG AGAATCAAGC 
CTAGGTCGAA AGGAAAATCA CTTGATCCAT TTGCCTCTTA CGTTGATTCT TGTCAACAGG 
AGAGGAGACA CAAGTAGTGA AACTGCACAT AGCCTCATTC AACAGGGCAA ACAGGTTGCC 
AGTAAGCTTC AGTGTTTCTT TCTTGATGCA GCTTCCACTG GAATGGGATA TGGTCGCAAT 
ATCAATGAGA AGCAAATAAG CCTAATCCTC AGAGGGCTAC TGGAGTCAAA AAGAAACTTA 
AACCTTGTCA GCTCCACTCC AAGCATTAAA GATTTGACAG ATTGTGAGCT GCGTATTGTA 
ATGTGCCTCA TGTGTGCAGA CATATTTAAC ATAGAAGATG TGCTGAATGC ACTAAAACCT 
CACACTTACC GTCCATCACA ATGTGGGCTT GGTGGATCAG TTCTACTTGA CCTACCAATT 
GGCAGTCAAA AACGTCGCTT GGAGCTGCTG TTGTTGTCGT ATCATGCCTC CTTTAATGTT 
CGCAAGACTC GTTTAGTGCA CGGCTATATG ATTTTTTACG CAGCTAAGCG CCGTGCTTCC 
CTAGCTATGC TGCGTGCCTT TTTGTGTGAA GTGCAGGACA TTGTCCCTGT GCAGCTTGTT 
GCCTTGACTG AGGGACCTCT AACATTTTTG GATTATGTGG CACGAGAACA GTTACAAGAA 
GGGGAGGAAA TAGCTCAGGA CATTGAAGGT AAATTCCTTA TTCTACCACA AGGGCAGATA 
CAGCCAAAGT TGGATGTGTT TTACCCCTAC TTCAAAGATG TATTGGAGAA AAAAGTAATT 
ATTGAGGCCT CTCACATGTA TGATAATACA GCAGAGGCCT GCAGCACCAC AGAGGAAGTG 
TTTAGCTCAC CCCGTGCAGG CTCACCCCTT TGTAATTCCC ACTTGCCAGA CTCTGAAGAA 
GACCCTGAAC CTTCACCTCA ACACATTTTA TTTCAAAATG AACCTAACAT AGCAGCTCCC 
ATAAAATTTA CCAGAGAACT GGAGGAGACT GTGATGAACA CTTTAGAGAG CAAACTTAAC 
AACAAGGTAC CTCCTCCCCT TAAACCAAAG CCCCCTATTC CATATGAAAT TACCAAGCCT 
GAGCTATCAT ATTTGGATAC AGGGGCCCGG GATGGGCACC GTAAGTCCTT GACTTCCATA 
AACTGGCCCC CTTTGGAGGC CTTTGACCCT TCTGACTATG CTGAACCTAT GGATTCAGTA 
GTGAAGCCAA GAAACCAAGA GGAGAACATT TACTCTGTAC CCCATGATAG CACCCAAGGA 
AAGATCATCA CTATACGTAA TACCAACAAA CCACAATCTA ATGGGAGTGG CAATGGCTCA 
GACAGTGATA TGGACACAAA CTCATTGGAA CGAGCAAGAA AAGCATCCAT TGTCACTAAA 
CCTGTCCTTT ACCGTACCAA ATGTGCTAAA CTTGGGAAAT TCTCTAGCTA CAGGAGTAGT 
TTAAGTGTTG GCAGTGATGA TGAACCTGGA CCAATTCGAA GAAAGGAAGA GGAGACTGGA 
ACACAGGGTA CAAAGGGGGA CAGTACTACG AATTCTTATG AAGCTGATGG AGAGGACCCA 
CGCAAGAGGA ATATTTTGCG CAGTCTGCGA AGGACCACTA AGAAAGTGAA ACCCAAACCT 
CGTCCATCTT TAACCAAGGC GACCTGGGAG AGTAATTACT TTGGTTTCCC TCTGAGTAGT 
GTGGTGACTT CTGAACGACC AATTCCAGTA TTCATTGAGA AGTGTGTAGA GTACATCGAA 
GCCACGGGAA TGACCACAGA AGGCATATAC CGTGTTAGCG GGAACAAATC TGAGATGGAG 
TCTCTTCAAA GGCAATTTGA CCAAGATCAT AACCTGGACC TGGCAGAAAA GGATTTTACA 
GTGAACACTG TGGCTGGAGC CTTGAAAAGT TTCTTCTCTG AATTACCGGA CCCTCTGGTG 
CCATACAATA TGCAGGCTGA GCTGGTGGAG GCATATAAAA TAAATGATCT AGAACATAAG 
CTCCAAGCTA TGAAGGATCT TCTAAAAAAA TTTCCAAAGG AAAATCATGA AATCTTCAAA 
TATGTTATAT CTCATTTAAA CCGGGTTAGC CAGCATCATC ATATAAATCT GATGACCAGT 
GAAAATTTAT CTATCTGCTT CTGGCCTACA CTGATGCGCC CTGATTTTAC CACTATGGAC 
GCTCTGACTG CAACTCGCAC ATACCAGACA ATCATTGAGC TTTTCATCCA ACAGTGCCCC 
TTTTTCTTCT ACCAGCGCCT TCCTGTGGAC CTGCCTATCC CTAGTTCCCC ATCTACTCCA 
CCTCTCACCC AGCCATCTCC TCCTCAGTCC CCTCCTCTGA CGCCTGTATC CCCCTCGCAG 
ACCCTGCTTC CTACGGAGCA CAACATCCTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002932643.1
CDS	551..4963
Translation	MMMARKPDVRVPTYSVSVVGLSGTEKEKGQCGVGKSCLCNRFVRPSADDFHLDHTSVLSTSDFGGRVVNNDHFLYWGESGRMPEDCVDFKVHVVEQTEFIDDQTFQPHRSTALQPYIKRAASCKLASAEKLMYFCTDQLGLEQDFEQKQMPEGKLLIDGFLLCIDVSRGMNRNFDDQLKFVSNLYTQLAKTKKPIVLVLTKCDEGVERYIRDAHSFALNKKNLQVVETSARSNVNVDLAFTTLVQLIDKSRGKSKIIPYFEALKQQSQLIAAAKDKYEWLVSRVVKSHNETWINQSRRMQSNIEYQDYVHLEGTDKAKKLFHQHVLRLKQEHIERRRRNYLLALPRALESLVPDLDEIDHLSWVKVSNLLESKAEFLKFFIVLEETPWDFTIHIDSIDERIPYDLLETVPAEEVYAAHLVKLRNARKKEEMKRSFKENLIASPFITPGKPWEEARSFIMSEEFYQWLEEPVYMEIYSRHQKEIIDKAKEEFQELLLEYSELFYELELDAKPSKEKMGVIQDVLGEEQRFKALQKLQAERDALILKHIHFVYHPTKDTCPSNPYCVDTRIEHILSSRCTYPYDRLHKDPNVDRINLVILGKDGLARELANEIRALCTNDDKYVIDGKMYELSLRPIEGNVRLPVNSFQTSTFLPHGCLCLYNSKESLSYVVESIEKSRESSLGRKENHLIHLPLTLILVNRRGDTSSETAHSLIQQGKQVASKLQCFFLDAASTGMGYGRNINEKQISLILRGLLESKRNLNLVSSTPSIKDLTDCELRIVMCLMCADIFNIEDVLNALKPHTYRPSQCGLGGSVLLDLPIGSQKRRLELLLLSYHASFNVRKTRLVHGYMIFYAAKRRASLAMLRAFLCEVQDIVPVQLVALTEGPLTFLDYVAREQLQEGEEIAQDIEGKFLILPQGQIQPKLDVFYPYFKDVLEKKVIIEASHMYDNTAEACSTTEEVFSSPRAGSPLCNSHLPDSEEDPEPSPQHILFQNEPNIAAPIKFTRELEETVMNTLESKLNNKVPPPLKPKPPIPYEITKPELSYLDTGARDGHRKSLTSINWPPLEAFDPSDYAEPMDSVVKPRNQEENIYSVPHDSTQGKIITIRNTNKPQSNGSGNGSDSDMDTNSLERARKASIVTKPVLYRTKCAKLGKFSSYRSSLSVGSDDEPGPIRRKEEETGTQGTKGDSTTNSYEADGEDPRKRNILRSLRRTTKKVKPKPRPSLTKATWESNYFGFPLSSVVTSERPIPVFIEKCVEYIEATGMTTEGIYRVSGNKSEMESLQRQFDQDHNLDLAEKDFTVNTVAGALKSFFSELPDPLVPYNMQAELVEAYKINDLEHKLQAMKDLLKKFPKENHEIFKYVISHLNRVSQHHHINLMTSENLSICFWPTLMRPDFTTMDALTATRTYQTIIELFIQQCPFFFYQRLPVDLPIPSSPSTPPLTQPSPPQSPPLTPVSPSQTLLPTEHNIL

Target ORF information:

RefSeq Version	XM_002932597.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho GTPase activating protein 35 (arhgap35), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002932597.5

ORF Insert Sequence:

ATGATGATGG CACGGAAACC AGATGTGCGT GTGCCCACCT ACAGTGTCAG CGTTGTGGGC 
CTTTCTGGCA CAGAGAAAGA GAAAGGCCAA TGTGGCGTTG GAAAATCATG CCTTTGTAAT 
CGATTTGTAA GACCCAGCGC AGATGATTTT CATCTGGACC ACACATCCGT CCTGAGCACT 
AGTGACTTTG GTGGTCGTGT TGTAAACAAT GACCATTTTC TGTACTGGGG AGAGAGTGGA 
CGGATGCCAG AAGACTGTGT AGACTTTAAA GTGCATGTGG TGGAGCAGAC AGAATTTATT 
GATGATCAGA CATTCCAGCC TCACCGTAGT ACTGCCCTAC AGCCTTACAT CAAGCGTGCT 
GCCTCCTGTA AGCTGGCCTC TGCTGAAAAA CTTATGTACT TCTGCACAGA TCAGTTGGGC 
TTGGAACAGG ACTTTGAGCA GAAGCAGATG CCTGAGGGTA AATTGTTGAT TGATGGTTTC 
CTGCTGTGTA TAGATGTTAG TCGTGGAATG AACCGGAACT TTGATGATCA GCTAAAATTT 
GTGTCCAATC TTTACACCCA GCTGGCCAAG ACAAAGAAAC CCATTGTTCT GGTTCTTACA 
AAATGTGATG AAGGAGTAGA ACGTTATATC CGCGATGCTC ATAGTTTTGC CCTTAACAAG 
AAGAACCTGC AAGTTGTAGA GACTTCTGCC CGGTCTAATG TGAATGTGGA TCTGGCCTTC 
ACTACACTAG TACAGCTTAT TGACAAAAGC CGTGGAAAGT CTAAAATTAT TCCTTATTTT 
GAGGCACTAA AGCAGCAGAG CCAACTGATT GCTGCCGCAA AGGACAAATA TGAATGGCTT 
GTGAGCCGAG TAGTAAAGTC TCATAATGAG ACATGGATCA ATCAAAGCAG GCGCATGCAG 
AGCAACATTG AATACCAAGA TTATGTGCAT TTGGAAGGAA CAGACAAAGC TAAGAAACTA 
TTCCATCAGC ACGTGCTACG GCTTAAACAG GAACACATAG AACGACGCAG AAGAAATTAC 
CTCTTAGCTC TCCCACGAGC CTTAGAAAGC CTTGTGCCAG ATCTGGATGA AATAGATCAC 
TTAAGCTGGG TTAAGGTTTC TAATTTGCTG GAATCAAAGG CAGAGTTCCT AAAATTTTTT 
ATTGTCCTGG AAGAGACACC TTGGGACTTC ACAATCCACA TTGACAGCAT TGATGAACGC 
ATACCTTATG ATTTGCTGGA AACAGTGCCT GCGGAAGAGG TCTATGCTGC CCACTTGGTC 
AAGTTAAGGA ATGCCAGGAA AAAGGAAGAA ATGAAGAGAT CTTTCAAGGA GAACTTGATC 
GCTTCTCCTT TCATTACACC AGGTAAGCCT TGGGAAGAGG CTCGGAGTTT TATCATGAGT 
GAAGAATTTT ACCAGTGGCT GGAAGAGCCT GTATATATGG AAATCTACAG CCGACACCAA 
AAAGAGATCA TTGATAAAGC TAAAGAGGAA TTTCAGGAGT TGTTGCTTGA GTATTCAGAG 
CTGTTTTATG AGCTAGAGTT AGATGCCAAA CCCAGTAAAG AGAAAATGGG CGTCATCCAG 
GATGTACTTG GTGAGGAACA ACGTTTCAAA GCATTACAGA AGCTGCAGGC GGAGCGAGAT 
GCCCTGATTC TTAAACACAT TCACTTTGTC TATCATCCAA CCAAGGACAC TTGTCCTAGC 
AACCCTTACT GTGTTGATAC AAGGATTGAG CATATACTCA GTTCCAGGTG CACATATCCC 
TATGACAGAC TACACAAAGA TCCAAATGTA GATAGGATCA ACCTTGTGAT CCTTGGAAAG 
GATGGTTTGG CACGAGAGTT AGCAAATGAG ATCCGAGCCC TCTGTACAAA TGATGATAAA 
TACGTAATTG ATGGAAAGAT GTATGAGTTA TCCCTAAGGC CTATAGAAGG CAATGTGCGG 
CTTCCTGTTA ATTCATTCCA GACATCCACT TTCCTCCCTC ATGGCTGTCT TTGCCTGTAC 
AACTCCAAGG AGTCTCTGTC CTATGTTGTG GAGAGCATTG AGAAAAGCAG AGAATCAAGC 
CTAGGTCGAA AGGAAAATCA CTTGATCCAT TTGCCTCTTA CGTTGATTCT TGTCAACAGG 
AGAGGAGACA CAAGTAGTGA AACTGCACAT AGCCTCATTC AACAGGGCAA ACAGGTTGCC 
AGTAAGCTTC AGTGTTTCTT TCTTGATGCA GCTTCCACTG GAATGGGATA TGGTCGCAAT 
ATCAATGAGA AGCAAATAAG CCTAATCCTC AGAGGGCTAC TGGAGTCAAA AAGAAACTTA 
AACCTTGTCA GCTCCACTCC AAGCATTAAA GATTTGACAG ATTGTGAGCT GCGTATTGTA 
ATGTGCCTCA TGTGTGCAGA CATATTTAAC ATAGAAGATG TGCTGAATGC ACTAAAACCT 
CACACTTACC GTCCATCACA ATGTGGGCTT GGTGGATCAG TTCTACTTGA CCTACCAATT 
GGCAGTCAAA AACGTCGCTT GGAGCTGCTG TTGTTGTCGT ATCATGCCTC CTTTAATGTT 
CGCAAGACTC GTTTAGTGCA CGGCTATATG ATTTTTTACG CAGCTAAGCG CCGTGCTTCC 
CTAGCTATGC TGCGTGCCTT TTTGTGTGAA GTGCAGGACA TTGTCCCTGT GCAGCTTGTT 
GCCTTGACTG AGGGACCTCT AACATTTTTG GATTATGTGG CACGAGAACA GTTACAAGAA 
GGGGAGGAAA TAGCTCAGGA CATTGAAGGT AAATTCCTTA TTCTACCACA AGGGCAGATA 
CAGCCAAAGT TGGATGTGTT TTACCCCTAC TTCAAAGATG TATTGGAGAA AAAAGTAATT 
ATTGAGGCCT CTCACATGTA TGATAATACA GCAGAGGCCT GCAGCACCAC AGAGGAAGTG 
TTTAGCTCAC CCCGTGCAGG CTCACCCCTT TGTAATTCCC ACTTGCCAGA CTCTGAAGAA 
GACCCTGAAC CTTCACCTCA ACACATTTTA TTTCAAAATG AACCTAACAT AGCAGCTCCC 
ATAAAATTTA CCAGAGAACT GGAGGAGACT GTGATGAACA CTTTAGAGAG CAAACTTAAC 
AACAAGGTAC CTCCTCCCCT TAAACCAAAG CCCCCTATTC CATATGAAAT TACCAAGCCT 
GAGCTATCAT ATTTGGATAC AGGGGCCCGG GATGGGCACC GTAAGTCCTT GACTTCCATA 
AACTGGCCCC CTTTGGAGGC CTTTGACCCT TCTGACTATG CTGAACCTAT GGATTCAGTA 
GTGAAGCCAA GAAACCAAGA GGAGAACATT TACTCTGTAC CCCATGATAG CACCCAAGGA 
AAGATCATCA CTATACGTAA TACCAACAAA CCACAATCTA ATGGGAGTGG CAATGGCTCA 
GACAGTGATA TGGACACAAA CTCATTGGAA CGAGCAAGAA AAGCATCCAT TGTCACTAAA 
CCTGTCCTTT ACCGTACCAA ATGTGCTAAA CTTGGGAAAT TCTCTAGCTA CAGGAGTAGT 
TTAAGTGTTG GCAGTGATGA TGAACCTGGA CCAATTCGAA GAAAGGAAGA GGAGACTGGA 
ACACAGGGTA CAAAGGGGGA CAGTACTACG AATTCTTATG AAGCTGATGG AGAGGACCCA 
CGCAAGAGGA ATATTTTGCG CAGTCTGCGA AGGACCACTA AGAAAGTGAA ACCCAAACCT 
CGTCCATCTT TAACCAAGGC GACCTGGGAG AGTAATTACT TTGGTTTCCC TCTGAGTAGT 
GTGGTGACTT CTGAACGACC AATTCCAGTA TTCATTGAGA AGTGTGTAGA GTACATCGAA 
GCCACGGGAA TGACCACAGA AGGCATATAC CGTGTTAGCG GGAACAAATC TGAGATGGAG 
TCTCTTCAAA GGCAATTTGA CCAAGATCAT AACCTGGACC TGGCAGAAAA GGATTTTACA 
GTGAACACTG TGGCTGGAGC CTTGAAAAGT TTCTTCTCTG AATTACCGGA CCCTCTGGTG 
CCATACAATA TGCAGGCTGA GCTGGTGGAG GCATATAAAA TAAATGATCT AGAACATAAG 
CTCCAAGCTA TGAAGGATCT TCTAAAAAAA TTTCCAAAGG AAAATCATGA AATCTTCAAA 
TATGTTATAT CTCATTTAAA CCGGGTTAGC CAGCATCATC ATATAAATCT GATGACCAGT 
GAAAATTTAT CTATCTGCTT CTGGCCTACA CTGATGCGCC CTGATTTTAC CACTATGGAC 
GCTCTGACTG CAACTCGCAC ATACCAGACA ATCATTGAGC TTTTCATCCA ACAGTGCCCC 
TTTTTCTTCT ACCAGCGCCT TCCTGTGGAC CTGCCTATCC CTAGTTCCCC ATCTACTCCA 
CCTCTCACCC AGCCATCTCC TCCTCAGTCC CCTCCTCTGA CGCCTGTATC CCCCTCGCAG 
ACCCTGCTTC CTACGGAGCA CAACATCCTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgap35 ( XM_018096665.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017952154.1 Xenopus tropicalis Rho GTPase activating protein 35 (arhgap35), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OXa46324
Clone ID Related Accession (Same CDS sequence)	XM_018096665.1
Accession Version	XM_018096665.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4185bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho GTPase-activating protein 35 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGG CACGGAAACC AGATGTGCGT GTGCCCACCT ACAGTGTCAG CGTTGTGGGC 
CTTTCTGGCA CAGAGAAAGA GAAAGGCCAA TGTGGCGTTG GAAAATCATG CCTTTGTAAT 
CGATTTGTAA GACCCAGCGC AGATGATTTT CATCTGGACC ACACATCCGT CCTGAGCACT 
AGTGACTTTG GTGGTCGTGT TGTAAACAAT GACCATTTTC TGTACTGGGG AGAGAGTGGA 
CGGATGCCAG AAGACTGTGT AGACTTTAAA GTGCATGTGG TGGAGCAGAC AGAATTTATT 
GATGATCAGA CATTCCAGCC TCACCGTAGT ACTGCCCTAC AGCCTTACAT CAAGCGTGCT 
GCCTCCTGTA AGCTGGCCTC TGCTGAAAAA CTTATGTACT TCTGCACAGA TCAGTTGGGC 
TTGGAACAGG ACTTTGAGCA GAAGCAGATG CCTGAGGGTA AATTGTTGAT TGATGGTTTC 
CTGCTGTGTA TAGATGTTAG TCGTGGAATG AACCGGAACT TTGATGATCA GCTAAAATTT 
GTGTCCAATC TTTACACCCA GCTGGCCAAG ACAAAGAAAC CCATTGTTCT GGTTCTTACA 
AAATGTGATG AAGGAGTAGA ACGTTATATC CGCGATGCTC ATAGTTTTGC CCTTAACAAG 
AAGAACCTGC AAGTTGTAGA GACTTCTGCC CGGTCTAATG TGAATGTGGA TCTGGCCTTC 
ACTACACTAG TACAGCTTAT TGACAAAAGC CGTGGAAAGT CTAAAATTAT TCCTTATTTT 
GAGGCACTAA AGCAGCAGAG CCAACTGATT GCTGCCGCAA AGGACAAATA TGAATGGCTT 
GTGAGCCGAG TAGTAAAGTC TCATAATGAG ACATGGATCA ATCAAAGCAG GCGCATGCAG 
AGCAACATTG AATACCAAGA TTATGTGCAT TTGGAAGGAA CAGACAAAGC TAAGAAACTA 
TTCCATCAGC ACGTGCTACG GCTTAAACAG GAACACATAG AACGACGCAG AAGAAATTAC 
CTCTTAGCTC TCCCACGAGC CTTAGAAAGC CTTGTGCCAG ATCTGGATGA AATAGATCAC 
TTAAGCTGGG TTAAGGTTTC TAATTTGCTG GAATCAAAGG CAGAGTTCCT AAAATTTTTT 
ATTGTCCTGG AAGAGACACC TTGGGACTTC ACAATCCACA TTGACAGCAT TGATGAACGC 
ATACCTTATG ATTTGCTGGA AACAGTGCCT GCGGAAGAGG TCTATGCTGC CCACTTGGTC 
AAGTTAAGGA ATGCCAGGAA AAAGGAAGAA ATGAAGAGAT CTTTCAAGGA GAACTTGATC 
GCTTCTCCTT TCATTACACC AGGTAAGCCT TGGGAAGAGG CTCGGAGTTT TATCATGAGT 
GAAGAATTTT ACCAGTGGCT GGAAGAGCCT GTATATATGG AAATCTACAG CCGACACCAA 
AAAGAGATCA TTGATAAAGC TAAAGAGGAA TTTCAGGAGT TGTTGCTTGA GTATTCAGAG 
CTGTTTTATG AGCTAGAGTT AGATGCCAAA CCCAGTAAAG AGAAAATGGG CGTCATCCAG 
GATGTACTTG GTGAGGAACA ACGTTTCAAA GCATTACAGA AGCTGCAGGC GGAGCGAGAT 
GCCCTGATTC TTAAACACAT TCACTTTGTC TATCATCCAA CCAAGGACAC TTGTCCTAGC 
AACCCTTACT GTGTTGATAC AAGGATTGAG CATATACTCA GTTCCAGGTG CACATATCCC 
TATGACAGAC TACACAAAGA TCCAAATGTA GATAGGATCA ACCTTGTGAT CCTTGGAAAG 
GATGGTTTGG CACGAGAGTT AGCAAATGAG ATCCGAGCCC TCTGTACAAA TGATGATAAA 
TACGTAATTG ATGGAAAGAT GTATGAGTTA TCCCTAAGGC CTATAGAAGG CAATGTGCGG 
CTTCCTGTTA ATTCATTCCA GACATCCACT TTCCTCCCTC ATGGCTGTCT TTGCCTGTAC 
AACTCCAAGG AGTCTCTGTC CTATGTTGTG GAGAGCATTG AGAAAAGCAG AGAATCAAGC 
CTAGGTCGAA AGGAAAATCA CTTGATCCAT TTGCCTCTTA CGTTGATTCT TGTCAACAGG 
AGAGGAGACA CAAGTAGTGA AACTGCACAT AGCCTCATTC AACAGGGCAA ACAGGTTGCC 
AGTAAGCTTC AGTGTTTCTT TCTTGATGCA GCTTCCACTG GAATGGGATA TGGTCGCAAT 
ATCAATGAGA AGCAAATAAG CCTAATCCTC AGAGGGCTAC TGGAGTCAAA AAGAAACTTA 
AACCTTGTCA GCTCCACTCC AAGCATTAAA GATTTGACAG ATTGTGAGCT GCGTATTGTA 
ATGTGCCTCA TGTGTGCAGA CATATTTAAC ATAGAAGATG TGCTGAATGC ACTAAAACCT 
CACACTTACC GTCCATCACA ATGTGGGCTT GGTGGATCAG TTCTACTTGA CCTACCAATT 
GGCAGTCAAA AACGTCGCTT GGAGCTGCTG TTGTTGTCGT ATCATGCCTC CTTTAATGTT 
CGCAAGACTC GTTTAGTGCA CGGCTATATG ATTTTTTACG CAGCTAAGCG CCGTGCTTCC 
CTAGCTATGC TGCGTGCCTT TTTGTGTGAA GTGCAGGACA TTGTCCCTGT GCAGCTTGTT 
GCCTTGACTG AGGGACCTCT AACATTTTTG GATTATGTGG CACGAGAACA GTTACAAGAA 
GGGGAGGAAA TAGCTCAGGA CATTGAAGGT AAATTCCTTA TTCTACCACA AGGGCAGATA 
CAGCCAAAGT TGGATGTGTT TTACCCCTAC TTCAAAGATG TATTGGAGAA AAAAGTAATT 
ATTGAGGCCT CTCACATGTA TGATAATACA GCAGAGGCCT GCAGCACCAC AGAGGAAGTG 
TTTAGCTCAC CCCGTGCAGG CTCACCCCTT TGTAATTCCC ACTTGCCAGA CTCTGAAGAA 
GACCCTGAAC CTTCACCTCA ACACATTTTA TTTCAAAATG AACCTAACAT AGCAGCTCCC 
ATAAAATTTA CCAGAGAACT GGAGGAGACT GTGATGAACA CTTTAGAGAG CAAACTTAAC 
AACAAGGTAC CTCCTCCCCT TAAACCAAAG CCCCCTATTC CATATGAAAT TACCAAGCCT 
GAGCTATCAT ATTTGGATAC AGGGGCCCGG GATGGGCACC GTAAGTCCTT GACTTCCATA 
AACTGGCCCC CTTTGGAGGC CTTTGACCCT TCTGACTATG CTGAACCTAT GGATTCAGTA 
GTGAAGCCAA GAAACCAAGA GGAGAACATT TACTCTGTAC CCCATGATAG CACCCAAGGA 
AAGATCATCA CTATACGTAA TACCAACAAA CCACAATCTA ATGGGAGTGG CAATGGCTCA 
GACAGTGATA TGGACACAAA CTCATTGGAA CGAGCAAGAA AAGCATCCAT TGTCACTAAA 
CCTGTCCTTT ACCGTACCAA ATGTGCTAAA CTTGGGAAAT TCTCTAGCTA CAGGAGTAGT 
TTAAGTGTTG GCAGTGATGA TGAACCTGGA CCAATTCGAA GAAAGGAAGA GGAGACTGGA 
ACACAGGGTA CAAAGGGGGA CAGTACTACG AATTCTTATG AAGCTGATGG AGAGGACCCA 
CGCAAGAGGA ATATTTTGCG CAGTCTGCGA AGGACCACTA AGAAAGTGAA ACCCAAACCT 
CGTCCATCTT TAACCAAGGC GACCTGGGAG AGTAATTACT TTGGTTTCCC TCTGAGTAGT 
GTGGTGACTT CTGAACGACC AATTCCAGTA TTCATTGAGA AGTGTGTAGA GTACATCGAA 
GCCACGGGTG AGGGAATGAC CACAGAAGGC ATATACCGTG TTAGCGGGAA CAAATCTGAG 
ATGGAGTCTC TTCAAAGGCA ATTTGACCAA GATCATAACC TGGACCTGGC AGAAAAGGAT 
TTTACAGTGA ACACTGTGGC TGGAGCCTTG AAAAGTTTCT TCTCTGAATT ACCGGACCCT 
CTGGTGCCAT ACAATATGCA GGCTGAGCTG GTGGAGGCAT ATAAAATAAA TGATCTAGAA 
CATAAGCTCC AAGCTATGAA GGATCTTCTA AAAAAATTTC CAAAGGAAAA TCATGAAATC 
TTCAAATATG TTATATCTCA TTTAAACCGG GATGGAAAGC TGTCAGTGAC TTCCACACTT 
CCTCTTCTAG TCAGTCTTTT AGAGTTGGCC TGCAGTGCTG TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017952154.1
CDS	562..4746
Translation	MMMARKPDVRVPTYSVSVVGLSGTEKEKGQCGVGKSCLCNRFVRPSADDFHLDHTSVLSTSDFGGRVVNNDHFLYWGESGRMPEDCVDFKVHVVEQTEFIDDQTFQPHRSTALQPYIKRAASCKLASAEKLMYFCTDQLGLEQDFEQKQMPEGKLLIDGFLLCIDVSRGMNRNFDDQLKFVSNLYTQLAKTKKPIVLVLTKCDEGVERYIRDAHSFALNKKNLQVVETSARSNVNVDLAFTTLVQLIDKSRGKSKIIPYFEALKQQSQLIAAAKDKYEWLVSRVVKSHNETWINQSRRMQSNIEYQDYVHLEGTDKAKKLFHQHVLRLKQEHIERRRRNYLLALPRALESLVPDLDEIDHLSWVKVSNLLESKAEFLKFFIVLEETPWDFTIHIDSIDERIPYDLLETVPAEEVYAAHLVKLRNARKKEEMKRSFKENLIASPFITPGKPWEEARSFIMSEEFYQWLEEPVYMEIYSRHQKEIIDKAKEEFQELLLEYSELFYELELDAKPSKEKMGVIQDVLGEEQRFKALQKLQAERDALILKHIHFVYHPTKDTCPSNPYCVDTRIEHILSSRCTYPYDRLHKDPNVDRINLVILGKDGLARELANEIRALCTNDDKYVIDGKMYELSLRPIEGNVRLPVNSFQTSTFLPHGCLCLYNSKESLSYVVESIEKSRESSLGRKENHLIHLPLTLILVNRRGDTSSETAHSLIQQGKQVASKLQCFFLDAASTGMGYGRNINEKQISLILRGLLESKRNLNLVSSTPSIKDLTDCELRIVMCLMCADIFNIEDVLNALKPHTYRPSQCGLGGSVLLDLPIGSQKRRLELLLLSYHASFNVRKTRLVHGYMIFYAAKRRASLAMLRAFLCEVQDIVPVQLVALTEGPLTFLDYVAREQLQEGEEIAQDIEGKFLILPQGQIQPKLDVFYPYFKDVLEKKVIIEASHMYDNTAEACSTTEEVFSSPRAGSPLCNSHLPDSEEDPEPSPQHILFQNEPNIAAPIKFTRELEETVMNTLESKLNNKVPPPLKPKPPIPYEITKPELSYLDTGARDGHRKSLTSINWPPLEAFDPSDYAEPMDSVVKPRNQEENIYSVPHDSTQGKIITIRNTNKPQSNGSGNGSDSDMDTNSLERARKASIVTKPVLYRTKCAKLGKFSSYRSSLSVGSDDEPGPIRRKEEETGTQGTKGDSTTNSYEADGEDPRKRNILRSLRRTTKKVKPKPRPSLTKATWESNYFGFPLSSVVTSERPIPVFIEKCVEYIEATGEGMTTEGIYRVSGNKSEMESLQRQFDQDHNLDLAEKDFTVNTVAGALKSFFSELPDPLVPYNMQAELVEAYKINDLEHKLQAMKDLLKKFPKENHEIFKYVISHLNRDGKLSVTSTLPLLVSLLELACSAV

Target ORF information:

RefSeq Version	XM_018096665.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho GTPase activating protein 35 (arhgap35), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018096665.1

ORF Insert Sequence:

ATGATGATGG CACGGAAACC AGATGTGCGT GTGCCCACCT ACAGTGTCAG CGTTGTGGGC 
CTTTCTGGCA CAGAGAAAGA GAAAGGCCAA TGTGGCGTTG GAAAATCATG CCTTTGTAAT 
CGATTTGTAA GACCCAGCGC AGATGATTTT CATCTGGACC ACACATCCGT CCTGAGCACT 
AGTGACTTTG GTGGTCGTGT TGTAAACAAT GACCATTTTC TGTACTGGGG AGAGAGTGGA 
CGGATGCCAG AAGACTGTGT AGACTTTAAA GTGCATGTGG TGGAGCAGAC AGAATTTATT 
GATGATCAGA CATTCCAGCC TCACCGTAGT ACTGCCCTAC AGCCTTACAT CAAGCGTGCT 
GCCTCCTGTA AGCTGGCCTC TGCTGAAAAA CTTATGTACT TCTGCACAGA TCAGTTGGGC 
TTGGAACAGG ACTTTGAGCA GAAGCAGATG CCTGAGGGTA AATTGTTGAT TGATGGTTTC 
CTGCTGTGTA TAGATGTTAG TCGTGGAATG AACCGGAACT TTGATGATCA GCTAAAATTT 
GTGTCCAATC TTTACACCCA GCTGGCCAAG ACAAAGAAAC CCATTGTTCT GGTTCTTACA 
AAATGTGATG AAGGAGTAGA ACGTTATATC CGCGATGCTC ATAGTTTTGC CCTTAACAAG 
AAGAACCTGC AAGTTGTAGA GACTTCTGCC CGGTCTAATG TGAATGTGGA TCTGGCCTTC 
ACTACACTAG TACAGCTTAT TGACAAAAGC CGTGGAAAGT CTAAAATTAT TCCTTATTTT 
GAGGCACTAA AGCAGCAGAG CCAACTGATT GCTGCCGCAA AGGACAAATA TGAATGGCTT 
GTGAGCCGAG TAGTAAAGTC TCATAATGAG ACATGGATCA ATCAAAGCAG GCGCATGCAG 
AGCAACATTG AATACCAAGA TTATGTGCAT TTGGAAGGAA CAGACAAAGC TAAGAAACTA 
TTCCATCAGC ACGTGCTACG GCTTAAACAG GAACACATAG AACGACGCAG AAGAAATTAC 
CTCTTAGCTC TCCCACGAGC CTTAGAAAGC CTTGTGCCAG ATCTGGATGA AATAGATCAC 
TTAAGCTGGG TTAAGGTTTC TAATTTGCTG GAATCAAAGG CAGAGTTCCT AAAATTTTTT 
ATTGTCCTGG AAGAGACACC TTGGGACTTC ACAATCCACA TTGACAGCAT TGATGAACGC 
ATACCTTATG ATTTGCTGGA AACAGTGCCT GCGGAAGAGG TCTATGCTGC CCACTTGGTC 
AAGTTAAGGA ATGCCAGGAA AAAGGAAGAA ATGAAGAGAT CTTTCAAGGA GAACTTGATC 
GCTTCTCCTT TCATTACACC AGGTAAGCCT TGGGAAGAGG CTCGGAGTTT TATCATGAGT 
GAAGAATTTT ACCAGTGGCT GGAAGAGCCT GTATATATGG AAATCTACAG CCGACACCAA 
AAAGAGATCA TTGATAAAGC TAAAGAGGAA TTTCAGGAGT TGTTGCTTGA GTATTCAGAG 
CTGTTTTATG AGCTAGAGTT AGATGCCAAA CCCAGTAAAG AGAAAATGGG CGTCATCCAG 
GATGTACTTG GTGAGGAACA ACGTTTCAAA GCATTACAGA AGCTGCAGGC GGAGCGAGAT 
GCCCTGATTC TTAAACACAT TCACTTTGTC TATCATCCAA CCAAGGACAC TTGTCCTAGC 
AACCCTTACT GTGTTGATAC AAGGATTGAG CATATACTCA GTTCCAGGTG CACATATCCC 
TATGACAGAC TACACAAAGA TCCAAATGTA GATAGGATCA ACCTTGTGAT CCTTGGAAAG 
GATGGTTTGG CACGAGAGTT AGCAAATGAG ATCCGAGCCC TCTGTACAAA TGATGATAAA 
TACGTAATTG ATGGAAAGAT GTATGAGTTA TCCCTAAGGC CTATAGAAGG CAATGTGCGG 
CTTCCTGTTA ATTCATTCCA GACATCCACT TTCCTCCCTC ATGGCTGTCT TTGCCTGTAC 
AACTCCAAGG AGTCTCTGTC CTATGTTGTG GAGAGCATTG AGAAAAGCAG AGAATCAAGC 
CTAGGTCGAA AGGAAAATCA CTTGATCCAT TTGCCTCTTA CGTTGATTCT TGTCAACAGG 
AGAGGAGACA CAAGTAGTGA AACTGCACAT AGCCTCATTC AACAGGGCAA ACAGGTTGCC 
AGTAAGCTTC AGTGTTTCTT TCTTGATGCA GCTTCCACTG GAATGGGATA TGGTCGCAAT 
ATCAATGAGA AGCAAATAAG CCTAATCCTC AGAGGGCTAC TGGAGTCAAA AAGAAACTTA 
AACCTTGTCA GCTCCACTCC AAGCATTAAA GATTTGACAG ATTGTGAGCT GCGTATTGTA 
ATGTGCCTCA TGTGTGCAGA CATATTTAAC ATAGAAGATG TGCTGAATGC ACTAAAACCT 
CACACTTACC GTCCATCACA ATGTGGGCTT GGTGGATCAG TTCTACTTGA CCTACCAATT 
GGCAGTCAAA AACGTCGCTT GGAGCTGCTG TTGTTGTCGT ATCATGCCTC CTTTAATGTT 
CGCAAGACTC GTTTAGTGCA CGGCTATATG ATTTTTTACG CAGCTAAGCG CCGTGCTTCC 
CTAGCTATGC TGCGTGCCTT TTTGTGTGAA GTGCAGGACA TTGTCCCTGT GCAGCTTGTT 
GCCTTGACTG AGGGACCTCT AACATTTTTG GATTATGTGG CACGAGAACA GTTACAAGAA 
GGGGAGGAAA TAGCTCAGGA CATTGAAGGT AAATTCCTTA TTCTACCACA AGGGCAGATA 
CAGCCAAAGT TGGATGTGTT TTACCCCTAC TTCAAAGATG TATTGGAGAA AAAAGTAATT 
ATTGAGGCCT CTCACATGTA TGATAATACA GCAGAGGCCT GCAGCACCAC AGAGGAAGTG 
TTTAGCTCAC CCCGTGCAGG CTCACCCCTT TGTAATTCCC ACTTGCCAGA CTCTGAAGAA 
GACCCTGAAC CTTCACCTCA ACACATTTTA TTTCAAAATG AACCTAACAT AGCAGCTCCC 
ATAAAATTTA CCAGAGAACT GGAGGAGACT GTGATGAACA CTTTAGAGAG CAAACTTAAC 
AACAAGGTAC CTCCTCCCCT TAAACCAAAG CCCCCTATTC CATATGAAAT TACCAAGCCT 
GAGCTATCAT ATTTGGATAC AGGGGCCCGG GATGGGCACC GTAAGTCCTT GACTTCCATA 
AACTGGCCCC CTTTGGAGGC CTTTGACCCT TCTGACTATG CTGAACCTAT GGATTCAGTA 
GTGAAGCCAA GAAACCAAGA GGAGAACATT TACTCTGTAC CCCATGATAG CACCCAAGGA 
AAGATCATCA CTATACGTAA TACCAACAAA CCACAATCTA ATGGGAGTGG CAATGGCTCA 
GACAGTGATA TGGACACAAA CTCATTGGAA CGAGCAAGAA AAGCATCCAT TGTCACTAAA 
CCTGTCCTTT ACCGTACCAA ATGTGCTAAA CTTGGGAAAT TCTCTAGCTA CAGGAGTAGT 
TTAAGTGTTG GCAGTGATGA TGAACCTGGA CCAATTCGAA GAAAGGAAGA GGAGACTGGA 
ACACAGGGTA CAAAGGGGGA CAGTACTACG AATTCTTATG AAGCTGATGG AGAGGACCCA 
CGCAAGAGGA ATATTTTGCG CAGTCTGCGA AGGACCACTA AGAAAGTGAA ACCCAAACCT 
CGTCCATCTT TAACCAAGGC GACCTGGGAG AGTAATTACT TTGGTTTCCC TCTGAGTAGT 
GTGGTGACTT CTGAACGACC AATTCCAGTA TTCATTGAGA AGTGTGTAGA GTACATCGAA 
GCCACGGGTG AGGGAATGAC CACAGAAGGC ATATACCGTG TTAGCGGGAA CAAATCTGAG 
ATGGAGTCTC TTCAAAGGCA ATTTGACCAA GATCATAACC TGGACCTGGC AGAAAAGGAT 
TTTACAGTGA ACACTGTGGC TGGAGCCTTG AAAAGTTTCT TCTCTGAATT ACCGGACCCT 
CTGGTGCCAT ACAATATGCA GGCTGAGCTG GTGGAGGCAT ATAAAATAAA TGATCTAGAA 
CATAAGCTCC AAGCTATGAA GGATCTTCTA AAAAAATTTC CAAAGGAAAA TCATGAAATC 
TTCAAATATG TTATATCTCA TTTAAACCGG GATGGAAAGC TGTCAGTGAC TTCCACACTT 
CCTCTTCTAG TCAGTCTTTT AGAGTTGGCC TGCAGTGCTG TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgap35 ( XM_004916953.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004917010.1 Xenopus tropicalis Rho GTPase activating protein 35 (arhgap35), transcript variant X3, mRNA.

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CloneID	OXa14506
Clone ID Related Accession (Same CDS sequence)	XM_004916953.3 , XM_004916951.4 , XM_004916952.4 , XM_004916951.3 , XM_004916952.3 , XM_004916953.4 , XM_031891234.1
Accession Version	XM_004916953.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4419bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho GTPase-activating protein 35 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004916953.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGG CACGGAAACC AGATGTGCGT GTGCCCACCT ACAGTGTCAG CGTTGTGGGC 
CTTTCTGGCA CAGAGAAAGA GAAAGGCCAA TGTGGCGTTG GAAAATCATG CCTTTGTAAT 
CGATTTGTAA GACCCAGCGC AGATGATTTT CATCTGGACC ACACATCCGT CCTGAGCACT 
AGTGACTTTG GTGGTCGTGT TGTAAACAAT GACCATTTTC TGTACTGGGG AGAGAGTGGA 
CGGATGCCAG AAGACTGTGT AGACTTTAAA GTGCATGTGG TGGAGCAGAC AGAATTTATT 
GATGATCAGA CATTCCAGCC TCACCGTAGT ACTGCCCTAC AGCCTTACAT CAAGCGTGCT 
GCCTCCTGTA AGCTGGCCTC TGCTGAAAAA CTTATGTACT TCTGCACAGA TCAGTTGGGC 
TTGGAACAGG ACTTTGAGCA GAAGCAGATG CCTGAGGGTA AATTGTTGAT TGATGGTTTC 
CTGCTGTGTA TAGATGTTAG TCGTGGAATG AACCGGAACT TTGATGATCA GCTAAAATTT 
GTGTCCAATC TTTACACCCA GCTGGCCAAG ACAAAGAAAC CCATTGTTCT GGTTCTTACA 
AAATGTGATG AAGGAGTAGA ACGTTATATC CGCGATGCTC ATAGTTTTGC CCTTAACAAG 
AAGAACCTGC AAGTTGTAGA GACTTCTGCC CGGTCTAATG TGAATGTGGA TCTGGCCTTC 
ACTACACTAG TACAGCTTAT TGACAAAAGC CGTGGAAAGT CTAAAATTAT TCCTTATTTT 
GAGGCACTAA AGCAGCAGAG CCAACTGATT GCTGCCGCAA AGGACAAATA TGAATGGCTT 
GTGAGCCGAG TAGTAAAGTC TCATAATGAG ACATGGATCA ATCAAAGCAG GCGCATGCAG 
AGCAACATTG AATACCAAGA TTATGTGCAT TTGGAAGGAA CAGACAAAGC TAAGAAACTA 
TTCCATCAGC ACGTGCTACG GCTTAAACAG GAACACATAG AACGACGCAG AAGAAATTAC 
CTCTTAGCTC TCCCACGAGC CTTAGAAAGC CTTGTGCCAG ATCTGGATGA AATAGATCAC 
TTAAGCTGGG TTAAGGTTTC TAATTTGCTG GAATCAAAGG CAGAGTTCCT AAAATTTTTT 
ATTGTCCTGG AAGAGACACC TTGGGACTTC ACAATCCACA TTGACAGCAT TGATGAACGC 
ATACCTTATG ATTTGCTGGA AACAGTGCCT GCGGAAGAGG TCTATGCTGC CCACTTGGTC 
AAGTTAAGGA ATGCCAGGAA AAAGGAAGAA ATGAAGAGAT CTTTCAAGGA GAACTTGATC 
GCTTCTCCTT TCATTACACC AGGTAAGCCT TGGGAAGAGG CTCGGAGTTT TATCATGAGT 
GAAGAATTTT ACCAGTGGCT GGAAGAGCCT GTATATATGG AAATCTACAG CCGACACCAA 
AAAGAGATCA TTGATAAAGC TAAAGAGGAA TTTCAGGAGT TGTTGCTTGA GTATTCAGAG 
CTGTTTTATG AGCTAGAGTT AGATGCCAAA CCCAGTAAAG AGAAAATGGG CGTCATCCAG 
GATGTACTTG GTGAGGAACA ACGTTTCAAA GCATTACAGA AGCTGCAGGC GGAGCGAGAT 
GCCCTGATTC TTAAACACAT TCACTTTGTC TATCATCCAA CCAAGGACAC TTGTCCTAGC 
AACCCTTACT GTGTTGATAC AAGGATTGAG CATATACTCA GTTCCAGGTG CACATATCCC 
TATGACAGAC TACACAAAGA TCCAAATGTA GATAGGATCA ACCTTGTGAT CCTTGGAAAG 
GATGGTTTGG CACGAGAGTT AGCAAATGAG ATCCGAGCCC TCTGTACAAA TGATGATAAA 
TACGTAATTG ATGGAAAGAT GTATGAGTTA TCCCTAAGGC CTATAGAAGG CAATGTGCGG 
CTTCCTGTTA ATTCATTCCA GACATCCACT TTCCTCCCTC ATGGCTGTCT TTGCCTGTAC 
AACTCCAAGG AGTCTCTGTC CTATGTTGTG GAGAGCATTG AGAAAAGCAG AGAATCAAGC 
CTAGGTCGAA AGGAAAATCA CTTGATCCAT TTGCCTCTTA CGTTGATTCT TGTCAACAGG 
AGAGGAGACA CAAGTAGTGA AACTGCACAT AGCCTCATTC AACAGGGCAA ACAGGTTGCC 
AGTAAGCTTC AGTGTTTCTT TCTTGATGCA GCTTCCACTG GAATGGGATA TGGTCGCAAT 
ATCAATGAGA AGCAAATAAG CCTAATCCTC AGAGGGCTAC TGGAGTCAAA AAGAAACTTA 
AACCTTGTCA GCTCCACTCC AAGCATTAAA GATTTGACAG ATTGTGAGCT GCGTATTGTA 
ATGTGCCTCA TGTGTGCAGA CATATTTAAC ATAGAAGATG TGCTGAATGC ACTAAAACCT 
CACACTTACC GTCCATCACA ATGTGGGCTT GGTGGATCAG TTCTACTTGA CCTACCAATT 
GGCAGTCAAA AACGTCGCTT GGAGCTGCTG TTGTTGTCGT ATCATGCCTC CTTTAATGTT 
CGCAAGACTC GTTTAGTGCA CGGCTATATG ATTTTTTACG CAGCTAAGCG CCGTGCTTCC 
CTAGCTATGC TGCGTGCCTT TTTGTGTGAA GTGCAGGACA TTGTCCCTGT GCAGCTTGTT 
GCCTTGACTG AGGGACCTCT AACATTTTTG GATTATGTGG CACGAGAACA GTTACAAGAA 
GGGGAGGAAA TAGCTCAGGA CATTGAAGGT AAATTCCTTA TTCTACCACA AGGGCAGATA 
CAGCCAAAGT TGGATGTGTT TTACCCCTAC TTCAAAGATG TATTGGAGAA AAAAGTAATT 
ATTGAGGCCT CTCACATGTA TGATAATACA GCAGAGGCCT GCAGCACCAC AGAGGAAGTG 
TTTAGCTCAC CCCGTGCAGG CTCACCCCTT TGTAATTCCC ACTTGCCAGA CTCTGAAGAA 
GACCCTGAAC CTTCACCTCA ACACATTTTA TTTCAAAATG AACCTAACAT AGCAGCTCCC 
ATAAAATTTA CCAGAGAACT GGAGGAGACT GTGATGAACA CTTTAGAGAG CAAACTTAAC 
AACAAGGTAC CTCCTCCCCT TAAACCAAAG CCCCCTATTC CATATGAAAT TACCAAGCCT 
GAGCTATCAT ATTTGGATAC AGGGGCCCGG GATGGGCACC GTAAGTCCTT GACTTCCATA 
AACTGGCCCC CTTTGGAGGC CTTTGACCCT TCTGACTATG CTGAACCTAT GGATTCAGTA 
GTGAAGCCAA GAAACCAAGA GGAGAACATT TACTCTGTAC CCCATGATAG CACCCAAGGA 
AAGATCATCA CTATACGTAA TACCAACAAA CCACAATCTA ATGGGAGTGG CAATGGCTCA 
GACAGTGATA TGGACACAAA CTCATTGGAA CGAGCAAGAA AAGCATCCAT TGTCACTAAA 
CCTGTCCTTT ACCGTACCAA ATGTGCTAAA CTTGGGAAAT TCTCTAGCTA CAGGAGTAGT 
TTAAGTGTTG GCAGTGATGA TGAACCTGGA CCAATTCGAA GAAAGGAAGA GGAGACTGGA 
ACACAGGGTA CAAAGGGGGA CAGTACTACG AATTCTTATG AAGCTGATGG AGAGGACCCA 
CGCAAGAGGA ATATTTTGCG CAGTCTGCGA AGGACCACTA AGAAAGTGAA ACCCAAACCT 
CGTCCATCTT TAACCAAGGC GACCTGGGAG AGTAATTACT TTGGTTTCCC TCTGAGTAGT 
GTGGTGACTT CTGAACGACC AATTCCAGTA TTCATTGAGA AGTGTGTAGA GTACATCGAA 
GCCACGGGTG AGGGAATGAC CACAGAAGGC ATATACCGTG TTAGCGGGAA CAAATCTGAG 
ATGGAGTCTC TTCAAAGGCA ATTTGACCAA GATCATAACC TGGACCTGGC AGAAAAGGAT 
TTTACAGTGA ACACTGTGGC TGGAGCCTTG AAAAGTTTCT TCTCTGAATT ACCGGACCCT 
CTGGTGCCAT ACAATATGCA GGCTGAGCTG GTGGAGGCAT ATAAAATAAA TGATCTAGAA 
CATAAGCTCC AAGCTATGAA GGATCTTCTA AAAAAATTTC CAAAGGAAAA TCATGAAATC 
TTCAAATATG TTATATCTCA TTTAAACCGG GTTAGCCAGC ATCATCATAT AAATCTGATG 
ACCAGTGAAA ATTTATCTAT CTGCTTCTGG CCTACACTGA TGCGCCCTGA TTTTACCACT 
ATGGACGCTC TGACTGCAAC TCGCACATAC CAGACAATCA TTGAGCTTTT CATCCAACAG 
TGCCCCTTTT TCTTCTACCA GCGCCTTCCT GTGGACCTGC CTATCCCTAG TTCCCCATCT 
ACTCCACCTC TCACCCAGCC ATCTCCTCCT CAGTCCCCTC CTCTGACGCC TGTATCCCCC 
TCGCAGACCC TGCTTCCTAC GGAGCACAAC ATCCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004917010.1
CDS	624..5042
Translation	MMMARKPDVRVPTYSVSVVGLSGTEKEKGQCGVGKSCLCNRFVRPSADDFHLDHTSVLSTSDFGGRVVNNDHFLYWGESGRMPEDCVDFKVHVVEQTEFIDDQTFQPHRSTALQPYIKRAASCKLASAEKLMYFCTDQLGLEQDFEQKQMPEGKLLIDGFLLCIDVSRGMNRNFDDQLKFVSNLYTQLAKTKKPIVLVLTKCDEGVERYIRDAHSFALNKKNLQVVETSARSNVNVDLAFTTLVQLIDKSRGKSKIIPYFEALKQQSQLIAAAKDKYEWLVSRVVKSHNETWINQSRRMQSNIEYQDYVHLEGTDKAKKLFHQHVLRLKQEHIERRRRNYLLALPRALESLVPDLDEIDHLSWVKVSNLLESKAEFLKFFIVLEETPWDFTIHIDSIDERIPYDLLETVPAEEVYAAHLVKLRNARKKEEMKRSFKENLIASPFITPGKPWEEARSFIMSEEFYQWLEEPVYMEIYSRHQKEIIDKAKEEFQELLLEYSELFYELELDAKPSKEKMGVIQDVLGEEQRFKALQKLQAERDALILKHIHFVYHPTKDTCPSNPYCVDTRIEHILSSRCTYPYDRLHKDPNVDRINLVILGKDGLARELANEIRALCTNDDKYVIDGKMYELSLRPIEGNVRLPVNSFQTSTFLPHGCLCLYNSKESLSYVVESIEKSRESSLGRKENHLIHLPLTLILVNRRGDTSSETAHSLIQQGKQVASKLQCFFLDAASTGMGYGRNINEKQISLILRGLLESKRNLNLVSSTPSIKDLTDCELRIVMCLMCADIFNIEDVLNALKPHTYRPSQCGLGGSVLLDLPIGSQKRRLELLLLSYHASFNVRKTRLVHGYMIFYAAKRRASLAMLRAFLCEVQDIVPVQLVALTEGPLTFLDYVAREQLQEGEEIAQDIEGKFLILPQGQIQPKLDVFYPYFKDVLEKKVIIEASHMYDNTAEACSTTEEVFSSPRAGSPLCNSHLPDSEEDPEPSPQHILFQNEPNIAAPIKFTRELEETVMNTLESKLNNKVPPPLKPKPPIPYEITKPELSYLDTGARDGHRKSLTSINWPPLEAFDPSDYAEPMDSVVKPRNQEENIYSVPHDSTQGKIITIRNTNKPQSNGSGNGSDSDMDTNSLERARKASIVTKPVLYRTKCAKLGKFSSYRSSLSVGSDDEPGPIRRKEEETGTQGTKGDSTTNSYEADGEDPRKRNILRSLRRTTKKVKPKPRPSLTKATWESNYFGFPLSSVVTSERPIPVFIEKCVEYIEATGEGMTTEGIYRVSGNKSEMESLQRQFDQDHNLDLAEKDFTVNTVAGALKSFFSELPDPLVPYNMQAELVEAYKINDLEHKLQAMKDLLKKFPKENHEIFKYVISHLNRVSQHHHINLMTSENLSICFWPTLMRPDFTTMDALTATRTYQTIIELFIQQCPFFFYQRLPVDLPIPSSPSTPPLTQPSPPQSPPLTPVSPSQTLLPTEHNIL

Target ORF information:

RefSeq Version	XM_004916953.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho GTPase activating protein 35 (arhgap35), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004916953.3

ORF Insert Sequence:

ATGATGATGG CACGGAAACC AGATGTGCGT GTGCCCACCT ACAGTGTCAG CGTTGTGGGC 
CTTTCTGGCA CAGAGAAAGA GAAAGGCCAA TGTGGCGTTG GAAAATCATG CCTTTGTAAT 
CGATTTGTAA GACCCAGCGC AGATGATTTT CATCTGGACC ACACATCCGT CCTGAGCACT 
AGTGACTTTG GTGGTCGTGT TGTAAACAAT GACCATTTTC TGTACTGGGG AGAGAGTGGA 
CGGATGCCAG AAGACTGTGT AGACTTTAAA GTGCATGTGG TGGAGCAGAC AGAATTTATT 
GATGATCAGA CATTCCAGCC TCACCGTAGT ACTGCCCTAC AGCCTTACAT CAAGCGTGCT 
GCCTCCTGTA AGCTGGCCTC TGCTGAAAAA CTTATGTACT TCTGCACAGA TCAGTTGGGC 
TTGGAACAGG ACTTTGAGCA GAAGCAGATG CCTGAGGGTA AATTGTTGAT TGATGGTTTC 
CTGCTGTGTA TAGATGTTAG TCGTGGAATG AACCGGAACT TTGATGATCA GCTAAAATTT 
GTGTCCAATC TTTACACCCA GCTGGCCAAG ACAAAGAAAC CCATTGTTCT GGTTCTTACA 
AAATGTGATG AAGGAGTAGA ACGTTATATC CGCGATGCTC ATAGTTTTGC CCTTAACAAG 
AAGAACCTGC AAGTTGTAGA GACTTCTGCC CGGTCTAATG TGAATGTGGA TCTGGCCTTC 
ACTACACTAG TACAGCTTAT TGACAAAAGC CGTGGAAAGT CTAAAATTAT TCCTTATTTT 
GAGGCACTAA AGCAGCAGAG CCAACTGATT GCTGCCGCAA AGGACAAATA TGAATGGCTT 
GTGAGCCGAG TAGTAAAGTC TCATAATGAG ACATGGATCA ATCAAAGCAG GCGCATGCAG 
AGCAACATTG AATACCAAGA TTATGTGCAT TTGGAAGGAA CAGACAAAGC TAAGAAACTA 
TTCCATCAGC ACGTGCTACG GCTTAAACAG GAACACATAG AACGACGCAG AAGAAATTAC 
CTCTTAGCTC TCCCACGAGC CTTAGAAAGC CTTGTGCCAG ATCTGGATGA AATAGATCAC 
TTAAGCTGGG TTAAGGTTTC TAATTTGCTG GAATCAAAGG CAGAGTTCCT AAAATTTTTT 
ATTGTCCTGG AAGAGACACC TTGGGACTTC ACAATCCACA TTGACAGCAT TGATGAACGC 
ATACCTTATG ATTTGCTGGA AACAGTGCCT GCGGAAGAGG TCTATGCTGC CCACTTGGTC 
AAGTTAAGGA ATGCCAGGAA AAAGGAAGAA ATGAAGAGAT CTTTCAAGGA GAACTTGATC 
GCTTCTCCTT TCATTACACC AGGTAAGCCT TGGGAAGAGG CTCGGAGTTT TATCATGAGT 
GAAGAATTTT ACCAGTGGCT GGAAGAGCCT GTATATATGG AAATCTACAG CCGACACCAA 
AAAGAGATCA TTGATAAAGC TAAAGAGGAA TTTCAGGAGT TGTTGCTTGA GTATTCAGAG 
CTGTTTTATG AGCTAGAGTT AGATGCCAAA CCCAGTAAAG AGAAAATGGG CGTCATCCAG 
GATGTACTTG GTGAGGAACA ACGTTTCAAA GCATTACAGA AGCTGCAGGC GGAGCGAGAT 
GCCCTGATTC TTAAACACAT TCACTTTGTC TATCATCCAA CCAAGGACAC TTGTCCTAGC 
AACCCTTACT GTGTTGATAC AAGGATTGAG CATATACTCA GTTCCAGGTG CACATATCCC 
TATGACAGAC TACACAAAGA TCCAAATGTA GATAGGATCA ACCTTGTGAT CCTTGGAAAG 
GATGGTTTGG CACGAGAGTT AGCAAATGAG ATCCGAGCCC TCTGTACAAA TGATGATAAA 
TACGTAATTG ATGGAAAGAT GTATGAGTTA TCCCTAAGGC CTATAGAAGG CAATGTGCGG 
CTTCCTGTTA ATTCATTCCA GACATCCACT TTCCTCCCTC ATGGCTGTCT TTGCCTGTAC 
AACTCCAAGG AGTCTCTGTC CTATGTTGTG GAGAGCATTG AGAAAAGCAG AGAATCAAGC 
CTAGGTCGAA AGGAAAATCA CTTGATCCAT TTGCCTCTTA CGTTGATTCT TGTCAACAGG 
AGAGGAGACA CAAGTAGTGA AACTGCACAT AGCCTCATTC AACAGGGCAA ACAGGTTGCC 
AGTAAGCTTC AGTGTTTCTT TCTTGATGCA GCTTCCACTG GAATGGGATA TGGTCGCAAT 
ATCAATGAGA AGCAAATAAG CCTAATCCTC AGAGGGCTAC TGGAGTCAAA AAGAAACTTA 
AACCTTGTCA GCTCCACTCC AAGCATTAAA GATTTGACAG ATTGTGAGCT GCGTATTGTA 
ATGTGCCTCA TGTGTGCAGA CATATTTAAC ATAGAAGATG TGCTGAATGC ACTAAAACCT 
CACACTTACC GTCCATCACA ATGTGGGCTT GGTGGATCAG TTCTACTTGA CCTACCAATT 
GGCAGTCAAA AACGTCGCTT GGAGCTGCTG TTGTTGTCGT ATCATGCCTC CTTTAATGTT 
CGCAAGACTC GTTTAGTGCA CGGCTATATG ATTTTTTACG CAGCTAAGCG CCGTGCTTCC 
CTAGCTATGC TGCGTGCCTT TTTGTGTGAA GTGCAGGACA TTGTCCCTGT GCAGCTTGTT 
GCCTTGACTG AGGGACCTCT AACATTTTTG GATTATGTGG CACGAGAACA GTTACAAGAA 
GGGGAGGAAA TAGCTCAGGA CATTGAAGGT AAATTCCTTA TTCTACCACA AGGGCAGATA 
CAGCCAAAGT TGGATGTGTT TTACCCCTAC TTCAAAGATG TATTGGAGAA AAAAGTAATT 
ATTGAGGCCT CTCACATGTA TGATAATACA GCAGAGGCCT GCAGCACCAC AGAGGAAGTG 
TTTAGCTCAC CCCGTGCAGG CTCACCCCTT TGTAATTCCC ACTTGCCAGA CTCTGAAGAA 
GACCCTGAAC CTTCACCTCA ACACATTTTA TTTCAAAATG AACCTAACAT AGCAGCTCCC 
ATAAAATTTA CCAGAGAACT GGAGGAGACT GTGATGAACA CTTTAGAGAG CAAACTTAAC 
AACAAGGTAC CTCCTCCCCT TAAACCAAAG CCCCCTATTC CATATGAAAT TACCAAGCCT 
GAGCTATCAT ATTTGGATAC AGGGGCCCGG GATGGGCACC GTAAGTCCTT GACTTCCATA 
AACTGGCCCC CTTTGGAGGC CTTTGACCCT TCTGACTATG CTGAACCTAT GGATTCAGTA 
GTGAAGCCAA GAAACCAAGA GGAGAACATT TACTCTGTAC CCCATGATAG CACCCAAGGA 
AAGATCATCA CTATACGTAA TACCAACAAA CCACAATCTA ATGGGAGTGG CAATGGCTCA 
GACAGTGATA TGGACACAAA CTCATTGGAA CGAGCAAGAA AAGCATCCAT TGTCACTAAA 
CCTGTCCTTT ACCGTACCAA ATGTGCTAAA CTTGGGAAAT TCTCTAGCTA CAGGAGTAGT 
TTAAGTGTTG GCAGTGATGA TGAACCTGGA CCAATTCGAA GAAAGGAAGA GGAGACTGGA 
ACACAGGGTA CAAAGGGGGA CAGTACTACG AATTCTTATG AAGCTGATGG AGAGGACCCA 
CGCAAGAGGA ATATTTTGCG CAGTCTGCGA AGGACCACTA AGAAAGTGAA ACCCAAACCT 
CGTCCATCTT TAACCAAGGC GACCTGGGAG AGTAATTACT TTGGTTTCCC TCTGAGTAGT 
GTGGTGACTT CTGAACGACC AATTCCAGTA TTCATTGAGA AGTGTGTAGA GTACATCGAA 
GCCACGGGTG AGGGAATGAC CACAGAAGGC ATATACCGTG TTAGCGGGAA CAAATCTGAG 
ATGGAGTCTC TTCAAAGGCA ATTTGACCAA GATCATAACC TGGACCTGGC AGAAAAGGAT 
TTTACAGTGA ACACTGTGGC TGGAGCCTTG AAAAGTTTCT TCTCTGAATT ACCGGACCCT 
CTGGTGCCAT ACAATATGCA GGCTGAGCTG GTGGAGGCAT ATAAAATAAA TGATCTAGAA 
CATAAGCTCC AAGCTATGAA GGATCTTCTA AAAAAATTTC CAAAGGAAAA TCATGAAATC 
TTCAAATATG TTATATCTCA TTTAAACCGG GTTAGCCAGC ATCATCATAT AAATCTGATG 
ACCAGTGAAA ATTTATCTAT CTGCTTCTGG CCTACACTGA TGCGCCCTGA TTTTACCACT 
ATGGACGCTC TGACTGCAAC TCGCACATAC CAGACAATCA TTGAGCTTTT CATCCAACAG 
TGCCCCTTTT TCTTCTACCA GCGCCTTCCT GTGGACCTGC CTATCCCTAG TTCCCCATCT 
ACTCCACCTC TCACCCAGCC ATCTCCTCCT CAGTCCCCTC CTCTGACGCC TGTATCCCCC 
TCGCAGACCC TGCTTCCTAC GGAGCACAAC ATCCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgap35 ( XM_004916951.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004917008.1 Xenopus tropicalis Rho GTPase activating protein 35 (arhgap35), transcript variant X1, mRNA.

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CloneID	OXa14506
Clone ID Related Accession (Same CDS sequence)	XM_004916953.3 , XM_004916951.4 , XM_004916952.4 , XM_004916951.3 , XM_004916952.3 , XM_004916953.4 , XM_031891234.1
Accession Version	XM_004916951.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4419bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho GTPase-activating protein 35 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004916951.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGG CACGGAAACC AGATGTGCGT GTGCCCACCT ACAGTGTCAG CGTTGTGGGC 
CTTTCTGGCA CAGAGAAAGA GAAAGGCCAA TGTGGCGTTG GAAAATCATG CCTTTGTAAT 
CGATTTGTAA GACCCAGCGC AGATGATTTT CATCTGGACC ACACATCCGT CCTGAGCACT 
AGTGACTTTG GTGGTCGTGT TGTAAACAAT GACCATTTTC TGTACTGGGG AGAGAGTGGA 
CGGATGCCAG AAGACTGTGT AGACTTTAAA GTGCATGTGG TGGAGCAGAC AGAATTTATT 
GATGATCAGA CATTCCAGCC TCACCGTAGT ACTGCCCTAC AGCCTTACAT CAAGCGTGCT 
GCCTCCTGTA AGCTGGCCTC TGCTGAAAAA CTTATGTACT TCTGCACAGA TCAGTTGGGC 
TTGGAACAGG ACTTTGAGCA GAAGCAGATG CCTGAGGGTA AATTGTTGAT TGATGGTTTC 
CTGCTGTGTA TAGATGTTAG TCGTGGAATG AACCGGAACT TTGATGATCA GCTAAAATTT 
GTGTCCAATC TTTACACCCA GCTGGCCAAG ACAAAGAAAC CCATTGTTCT GGTTCTTACA 
AAATGTGATG AAGGAGTAGA ACGTTATATC CGCGATGCTC ATAGTTTTGC CCTTAACAAG 
AAGAACCTGC AAGTTGTAGA GACTTCTGCC CGGTCTAATG TGAATGTGGA TCTGGCCTTC 
ACTACACTAG TACAGCTTAT TGACAAAAGC CGTGGAAAGT CTAAAATTAT TCCTTATTTT 
GAGGCACTAA AGCAGCAGAG CCAACTGATT GCTGCCGCAA AGGACAAATA TGAATGGCTT 
GTGAGCCGAG TAGTAAAGTC TCATAATGAG ACATGGATCA ATCAAAGCAG GCGCATGCAG 
AGCAACATTG AATACCAAGA TTATGTGCAT TTGGAAGGAA CAGACAAAGC TAAGAAACTA 
TTCCATCAGC ACGTGCTACG GCTTAAACAG GAACACATAG AACGACGCAG AAGAAATTAC 
CTCTTAGCTC TCCCACGAGC CTTAGAAAGC CTTGTGCCAG ATCTGGATGA AATAGATCAC 
TTAAGCTGGG TTAAGGTTTC TAATTTGCTG GAATCAAAGG CAGAGTTCCT AAAATTTTTT 
ATTGTCCTGG AAGAGACACC TTGGGACTTC ACAATCCACA TTGACAGCAT TGATGAACGC 
ATACCTTATG ATTTGCTGGA AACAGTGCCT GCGGAAGAGG TCTATGCTGC CCACTTGGTC 
AAGTTAAGGA ATGCCAGGAA AAAGGAAGAA ATGAAGAGAT CTTTCAAGGA GAACTTGATC 
GCTTCTCCTT TCATTACACC AGGTAAGCCT TGGGAAGAGG CTCGGAGTTT TATCATGAGT 
GAAGAATTTT ACCAGTGGCT GGAAGAGCCT GTATATATGG AAATCTACAG CCGACACCAA 
AAAGAGATCA TTGATAAAGC TAAAGAGGAA TTTCAGGAGT TGTTGCTTGA GTATTCAGAG 
CTGTTTTATG AGCTAGAGTT AGATGCCAAA CCCAGTAAAG AGAAAATGGG CGTCATCCAG 
GATGTACTTG GTGAGGAACA ACGTTTCAAA GCATTACAGA AGCTGCAGGC GGAGCGAGAT 
GCCCTGATTC TTAAACACAT TCACTTTGTC TATCATCCAA CCAAGGACAC TTGTCCTAGC 
AACCCTTACT GTGTTGATAC AAGGATTGAG CATATACTCA GTTCCAGGTG CACATATCCC 
TATGACAGAC TACACAAAGA TCCAAATGTA GATAGGATCA ACCTTGTGAT CCTTGGAAAG 
GATGGTTTGG CACGAGAGTT AGCAAATGAG ATCCGAGCCC TCTGTACAAA TGATGATAAA 
TACGTAATTG ATGGAAAGAT GTATGAGTTA TCCCTAAGGC CTATAGAAGG CAATGTGCGG 
CTTCCTGTTA ATTCATTCCA GACATCCACT TTCCTCCCTC ATGGCTGTCT TTGCCTGTAC 
AACTCCAAGG AGTCTCTGTC CTATGTTGTG GAGAGCATTG AGAAAAGCAG AGAATCAAGC 
CTAGGTCGAA AGGAAAATCA CTTGATCCAT TTGCCTCTTA CGTTGATTCT TGTCAACAGG 
AGAGGAGACA CAAGTAGTGA AACTGCACAT AGCCTCATTC AACAGGGCAA ACAGGTTGCC 
AGTAAGCTTC AGTGTTTCTT TCTTGATGCA GCTTCCACTG GAATGGGATA TGGTCGCAAT 
ATCAATGAGA AGCAAATAAG CCTAATCCTC AGAGGGCTAC TGGAGTCAAA AAGAAACTTA 
AACCTTGTCA GCTCCACTCC AAGCATTAAA GATTTGACAG ATTGTGAGCT GCGTATTGTA 
ATGTGCCTCA TGTGTGCAGA CATATTTAAC ATAGAAGATG TGCTGAATGC ACTAAAACCT 
CACACTTACC GTCCATCACA ATGTGGGCTT GGTGGATCAG TTCTACTTGA CCTACCAATT 
GGCAGTCAAA AACGTCGCTT GGAGCTGCTG TTGTTGTCGT ATCATGCCTC CTTTAATGTT 
CGCAAGACTC GTTTAGTGCA CGGCTATATG ATTTTTTACG CAGCTAAGCG CCGTGCTTCC 
CTAGCTATGC TGCGTGCCTT TTTGTGTGAA GTGCAGGACA TTGTCCCTGT GCAGCTTGTT 
GCCTTGACTG AGGGACCTCT AACATTTTTG GATTATGTGG CACGAGAACA GTTACAAGAA 
GGGGAGGAAA TAGCTCAGGA CATTGAAGGT AAATTCCTTA TTCTACCACA AGGGCAGATA 
CAGCCAAAGT TGGATGTGTT TTACCCCTAC TTCAAAGATG TATTGGAGAA AAAAGTAATT 
ATTGAGGCCT CTCACATGTA TGATAATACA GCAGAGGCCT GCAGCACCAC AGAGGAAGTG 
TTTAGCTCAC CCCGTGCAGG CTCACCCCTT TGTAATTCCC ACTTGCCAGA CTCTGAAGAA 
GACCCTGAAC CTTCACCTCA ACACATTTTA TTTCAAAATG AACCTAACAT AGCAGCTCCC 
ATAAAATTTA CCAGAGAACT GGAGGAGACT GTGATGAACA CTTTAGAGAG CAAACTTAAC 
AACAAGGTAC CTCCTCCCCT TAAACCAAAG CCCCCTATTC CATATGAAAT TACCAAGCCT 
GAGCTATCAT ATTTGGATAC AGGGGCCCGG GATGGGCACC GTAAGTCCTT GACTTCCATA 
AACTGGCCCC CTTTGGAGGC CTTTGACCCT TCTGACTATG CTGAACCTAT GGATTCAGTA 
GTGAAGCCAA GAAACCAAGA GGAGAACATT TACTCTGTAC CCCATGATAG CACCCAAGGA 
AAGATCATCA CTATACGTAA TACCAACAAA CCACAATCTA ATGGGAGTGG CAATGGCTCA 
GACAGTGATA TGGACACAAA CTCATTGGAA CGAGCAAGAA AAGCATCCAT TGTCACTAAA 
CCTGTCCTTT ACCGTACCAA ATGTGCTAAA CTTGGGAAAT TCTCTAGCTA CAGGAGTAGT 
TTAAGTGTTG GCAGTGATGA TGAACCTGGA CCAATTCGAA GAAAGGAAGA GGAGACTGGA 
ACACAGGGTA CAAAGGGGGA CAGTACTACG AATTCTTATG AAGCTGATGG AGAGGACCCA 
CGCAAGAGGA ATATTTTGCG CAGTCTGCGA AGGACCACTA AGAAAGTGAA ACCCAAACCT 
CGTCCATCTT TAACCAAGGC GACCTGGGAG AGTAATTACT TTGGTTTCCC TCTGAGTAGT 
GTGGTGACTT CTGAACGACC AATTCCAGTA TTCATTGAGA AGTGTGTAGA GTACATCGAA 
GCCACGGGTG AGGGAATGAC CACAGAAGGC ATATACCGTG TTAGCGGGAA CAAATCTGAG 
ATGGAGTCTC TTCAAAGGCA ATTTGACCAA GATCATAACC TGGACCTGGC AGAAAAGGAT 
TTTACAGTGA ACACTGTGGC TGGAGCCTTG AAAAGTTTCT TCTCTGAATT ACCGGACCCT 
CTGGTGCCAT ACAATATGCA GGCTGAGCTG GTGGAGGCAT ATAAAATAAA TGATCTAGAA 
CATAAGCTCC AAGCTATGAA GGATCTTCTA AAAAAATTTC CAAAGGAAAA TCATGAAATC 
TTCAAATATG TTATATCTCA TTTAAACCGG GTTAGCCAGC ATCATCATAT AAATCTGATG 
ACCAGTGAAA ATTTATCTAT CTGCTTCTGG CCTACACTGA TGCGCCCTGA TTTTACCACT 
ATGGACGCTC TGACTGCAAC TCGCACATAC CAGACAATCA TTGAGCTTTT CATCCAACAG 
TGCCCCTTTT TCTTCTACCA GCGCCTTCCT GTGGACCTGC CTATCCCTAG TTCCCCATCT 
ACTCCACCTC TCACCCAGCC ATCTCCTCCT CAGTCCCCTC CTCTGACGCC TGTATCCCCC 
TCGCAGACCC TGCTTCCTAC GGAGCACAAC ATCCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004917008.1
CDS	560..4978
Translation	MMMARKPDVRVPTYSVSVVGLSGTEKEKGQCGVGKSCLCNRFVRPSADDFHLDHTSVLSTSDFGGRVVNNDHFLYWGESGRMPEDCVDFKVHVVEQTEFIDDQTFQPHRSTALQPYIKRAASCKLASAEKLMYFCTDQLGLEQDFEQKQMPEGKLLIDGFLLCIDVSRGMNRNFDDQLKFVSNLYTQLAKTKKPIVLVLTKCDEGVERYIRDAHSFALNKKNLQVVETSARSNVNVDLAFTTLVQLIDKSRGKSKIIPYFEALKQQSQLIAAAKDKYEWLVSRVVKSHNETWINQSRRMQSNIEYQDYVHLEGTDKAKKLFHQHVLRLKQEHIERRRRNYLLALPRALESLVPDLDEIDHLSWVKVSNLLESKAEFLKFFIVLEETPWDFTIHIDSIDERIPYDLLETVPAEEVYAAHLVKLRNARKKEEMKRSFKENLIASPFITPGKPWEEARSFIMSEEFYQWLEEPVYMEIYSRHQKEIIDKAKEEFQELLLEYSELFYELELDAKPSKEKMGVIQDVLGEEQRFKALQKLQAERDALILKHIHFVYHPTKDTCPSNPYCVDTRIEHILSSRCTYPYDRLHKDPNVDRINLVILGKDGLARELANEIRALCTNDDKYVIDGKMYELSLRPIEGNVRLPVNSFQTSTFLPHGCLCLYNSKESLSYVVESIEKSRESSLGRKENHLIHLPLTLILVNRRGDTSSETAHSLIQQGKQVASKLQCFFLDAASTGMGYGRNINEKQISLILRGLLESKRNLNLVSSTPSIKDLTDCELRIVMCLMCADIFNIEDVLNALKPHTYRPSQCGLGGSVLLDLPIGSQKRRLELLLLSYHASFNVRKTRLVHGYMIFYAAKRRASLAMLRAFLCEVQDIVPVQLVALTEGPLTFLDYVAREQLQEGEEIAQDIEGKFLILPQGQIQPKLDVFYPYFKDVLEKKVIIEASHMYDNTAEACSTTEEVFSSPRAGSPLCNSHLPDSEEDPEPSPQHILFQNEPNIAAPIKFTRELEETVMNTLESKLNNKVPPPLKPKPPIPYEITKPELSYLDTGARDGHRKSLTSINWPPLEAFDPSDYAEPMDSVVKPRNQEENIYSVPHDSTQGKIITIRNTNKPQSNGSGNGSDSDMDTNSLERARKASIVTKPVLYRTKCAKLGKFSSYRSSLSVGSDDEPGPIRRKEEETGTQGTKGDSTTNSYEADGEDPRKRNILRSLRRTTKKVKPKPRPSLTKATWESNYFGFPLSSVVTSERPIPVFIEKCVEYIEATGEGMTTEGIYRVSGNKSEMESLQRQFDQDHNLDLAEKDFTVNTVAGALKSFFSELPDPLVPYNMQAELVEAYKINDLEHKLQAMKDLLKKFPKENHEIFKYVISHLNRVSQHHHINLMTSENLSICFWPTLMRPDFTTMDALTATRTYQTIIELFIQQCPFFFYQRLPVDLPIPSSPSTPPLTQPSPPQSPPLTPVSPSQTLLPTEHNIL

Target ORF information:

RefSeq Version	XM_004916951.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho GTPase activating protein 35 (arhgap35), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004916951.3

ORF Insert Sequence:

ATGATGATGG CACGGAAACC AGATGTGCGT GTGCCCACCT ACAGTGTCAG CGTTGTGGGC 
CTTTCTGGCA CAGAGAAAGA GAAAGGCCAA TGTGGCGTTG GAAAATCATG CCTTTGTAAT 
CGATTTGTAA GACCCAGCGC AGATGATTTT CATCTGGACC ACACATCCGT CCTGAGCACT 
AGTGACTTTG GTGGTCGTGT TGTAAACAAT GACCATTTTC TGTACTGGGG AGAGAGTGGA 
CGGATGCCAG AAGACTGTGT AGACTTTAAA GTGCATGTGG TGGAGCAGAC AGAATTTATT 
GATGATCAGA CATTCCAGCC TCACCGTAGT ACTGCCCTAC AGCCTTACAT CAAGCGTGCT 
GCCTCCTGTA AGCTGGCCTC TGCTGAAAAA CTTATGTACT TCTGCACAGA TCAGTTGGGC 
TTGGAACAGG ACTTTGAGCA GAAGCAGATG CCTGAGGGTA AATTGTTGAT TGATGGTTTC 
CTGCTGTGTA TAGATGTTAG TCGTGGAATG AACCGGAACT TTGATGATCA GCTAAAATTT 
GTGTCCAATC TTTACACCCA GCTGGCCAAG ACAAAGAAAC CCATTGTTCT GGTTCTTACA 
AAATGTGATG AAGGAGTAGA ACGTTATATC CGCGATGCTC ATAGTTTTGC CCTTAACAAG 
AAGAACCTGC AAGTTGTAGA GACTTCTGCC CGGTCTAATG TGAATGTGGA TCTGGCCTTC 
ACTACACTAG TACAGCTTAT TGACAAAAGC CGTGGAAAGT CTAAAATTAT TCCTTATTTT 
GAGGCACTAA AGCAGCAGAG CCAACTGATT GCTGCCGCAA AGGACAAATA TGAATGGCTT 
GTGAGCCGAG TAGTAAAGTC TCATAATGAG ACATGGATCA ATCAAAGCAG GCGCATGCAG 
AGCAACATTG AATACCAAGA TTATGTGCAT TTGGAAGGAA CAGACAAAGC TAAGAAACTA 
TTCCATCAGC ACGTGCTACG GCTTAAACAG GAACACATAG AACGACGCAG AAGAAATTAC 
CTCTTAGCTC TCCCACGAGC CTTAGAAAGC CTTGTGCCAG ATCTGGATGA AATAGATCAC 
TTAAGCTGGG TTAAGGTTTC TAATTTGCTG GAATCAAAGG CAGAGTTCCT AAAATTTTTT 
ATTGTCCTGG AAGAGACACC TTGGGACTTC ACAATCCACA TTGACAGCAT TGATGAACGC 
ATACCTTATG ATTTGCTGGA AACAGTGCCT GCGGAAGAGG TCTATGCTGC CCACTTGGTC 
AAGTTAAGGA ATGCCAGGAA AAAGGAAGAA ATGAAGAGAT CTTTCAAGGA GAACTTGATC 
GCTTCTCCTT TCATTACACC AGGTAAGCCT TGGGAAGAGG CTCGGAGTTT TATCATGAGT 
GAAGAATTTT ACCAGTGGCT GGAAGAGCCT GTATATATGG AAATCTACAG CCGACACCAA 
AAAGAGATCA TTGATAAAGC TAAAGAGGAA TTTCAGGAGT TGTTGCTTGA GTATTCAGAG 
CTGTTTTATG AGCTAGAGTT AGATGCCAAA CCCAGTAAAG AGAAAATGGG CGTCATCCAG 
GATGTACTTG GTGAGGAACA ACGTTTCAAA GCATTACAGA AGCTGCAGGC GGAGCGAGAT 
GCCCTGATTC TTAAACACAT TCACTTTGTC TATCATCCAA CCAAGGACAC TTGTCCTAGC 
AACCCTTACT GTGTTGATAC AAGGATTGAG CATATACTCA GTTCCAGGTG CACATATCCC 
TATGACAGAC TACACAAAGA TCCAAATGTA GATAGGATCA ACCTTGTGAT CCTTGGAAAG 
GATGGTTTGG CACGAGAGTT AGCAAATGAG ATCCGAGCCC TCTGTACAAA TGATGATAAA 
TACGTAATTG ATGGAAAGAT GTATGAGTTA TCCCTAAGGC CTATAGAAGG CAATGTGCGG 
CTTCCTGTTA ATTCATTCCA GACATCCACT TTCCTCCCTC ATGGCTGTCT TTGCCTGTAC 
AACTCCAAGG AGTCTCTGTC CTATGTTGTG GAGAGCATTG AGAAAAGCAG AGAATCAAGC 
CTAGGTCGAA AGGAAAATCA CTTGATCCAT TTGCCTCTTA CGTTGATTCT TGTCAACAGG 
AGAGGAGACA CAAGTAGTGA AACTGCACAT AGCCTCATTC AACAGGGCAA ACAGGTTGCC 
AGTAAGCTTC AGTGTTTCTT TCTTGATGCA GCTTCCACTG GAATGGGATA TGGTCGCAAT 
ATCAATGAGA AGCAAATAAG CCTAATCCTC AGAGGGCTAC TGGAGTCAAA AAGAAACTTA 
AACCTTGTCA GCTCCACTCC AAGCATTAAA GATTTGACAG ATTGTGAGCT GCGTATTGTA 
ATGTGCCTCA TGTGTGCAGA CATATTTAAC ATAGAAGATG TGCTGAATGC ACTAAAACCT 
CACACTTACC GTCCATCACA ATGTGGGCTT GGTGGATCAG TTCTACTTGA CCTACCAATT 
GGCAGTCAAA AACGTCGCTT GGAGCTGCTG TTGTTGTCGT ATCATGCCTC CTTTAATGTT 
CGCAAGACTC GTTTAGTGCA CGGCTATATG ATTTTTTACG CAGCTAAGCG CCGTGCTTCC 
CTAGCTATGC TGCGTGCCTT TTTGTGTGAA GTGCAGGACA TTGTCCCTGT GCAGCTTGTT 
GCCTTGACTG AGGGACCTCT AACATTTTTG GATTATGTGG CACGAGAACA GTTACAAGAA 
GGGGAGGAAA TAGCTCAGGA CATTGAAGGT AAATTCCTTA TTCTACCACA AGGGCAGATA 
CAGCCAAAGT TGGATGTGTT TTACCCCTAC TTCAAAGATG TATTGGAGAA AAAAGTAATT 
ATTGAGGCCT CTCACATGTA TGATAATACA GCAGAGGCCT GCAGCACCAC AGAGGAAGTG 
TTTAGCTCAC CCCGTGCAGG CTCACCCCTT TGTAATTCCC ACTTGCCAGA CTCTGAAGAA 
GACCCTGAAC CTTCACCTCA ACACATTTTA TTTCAAAATG AACCTAACAT AGCAGCTCCC 
ATAAAATTTA CCAGAGAACT GGAGGAGACT GTGATGAACA CTTTAGAGAG CAAACTTAAC 
AACAAGGTAC CTCCTCCCCT TAAACCAAAG CCCCCTATTC CATATGAAAT TACCAAGCCT 
GAGCTATCAT ATTTGGATAC AGGGGCCCGG GATGGGCACC GTAAGTCCTT GACTTCCATA 
AACTGGCCCC CTTTGGAGGC CTTTGACCCT TCTGACTATG CTGAACCTAT GGATTCAGTA 
GTGAAGCCAA GAAACCAAGA GGAGAACATT TACTCTGTAC CCCATGATAG CACCCAAGGA 
AAGATCATCA CTATACGTAA TACCAACAAA CCACAATCTA ATGGGAGTGG CAATGGCTCA 
GACAGTGATA TGGACACAAA CTCATTGGAA CGAGCAAGAA AAGCATCCAT TGTCACTAAA 
CCTGTCCTTT ACCGTACCAA ATGTGCTAAA CTTGGGAAAT TCTCTAGCTA CAGGAGTAGT 
TTAAGTGTTG GCAGTGATGA TGAACCTGGA CCAATTCGAA GAAAGGAAGA GGAGACTGGA 
ACACAGGGTA CAAAGGGGGA CAGTACTACG AATTCTTATG AAGCTGATGG AGAGGACCCA 
CGCAAGAGGA ATATTTTGCG CAGTCTGCGA AGGACCACTA AGAAAGTGAA ACCCAAACCT 
CGTCCATCTT TAACCAAGGC GACCTGGGAG AGTAATTACT TTGGTTTCCC TCTGAGTAGT 
GTGGTGACTT CTGAACGACC AATTCCAGTA TTCATTGAGA AGTGTGTAGA GTACATCGAA 
GCCACGGGTG AGGGAATGAC CACAGAAGGC ATATACCGTG TTAGCGGGAA CAAATCTGAG 
ATGGAGTCTC TTCAAAGGCA ATTTGACCAA GATCATAACC TGGACCTGGC AGAAAAGGAT 
TTTACAGTGA ACACTGTGGC TGGAGCCTTG AAAAGTTTCT TCTCTGAATT ACCGGACCCT 
CTGGTGCCAT ACAATATGCA GGCTGAGCTG GTGGAGGCAT ATAAAATAAA TGATCTAGAA 
CATAAGCTCC AAGCTATGAA GGATCTTCTA AAAAAATTTC CAAAGGAAAA TCATGAAATC 
TTCAAATATG TTATATCTCA TTTAAACCGG GTTAGCCAGC ATCATCATAT AAATCTGATG 
ACCAGTGAAA ATTTATCTAT CTGCTTCTGG CCTACACTGA TGCGCCCTGA TTTTACCACT 
ATGGACGCTC TGACTGCAAC TCGCACATAC CAGACAATCA TTGAGCTTTT CATCCAACAG 
TGCCCCTTTT TCTTCTACCA GCGCCTTCCT GTGGACCTGC CTATCCCTAG TTCCCCATCT 
ACTCCACCTC TCACCCAGCC ATCTCCTCCT CAGTCCCCTC CTCTGACGCC TGTATCCCCC 
TCGCAGACCC TGCTTCCTAC GGAGCACAAC ATCCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgap35 ( XM_004916952.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_004917009.1 Xenopus tropicalis Rho GTPase activating protein 35 (arhgap35), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa14506
Clone ID Related Accession (Same CDS sequence)	XM_004916953.3 , XM_004916951.4 , XM_004916952.4 , XM_004916951.3 , XM_004916952.3 , XM_004916953.4 , XM_031891234.1
Accession Version	XM_004916952.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4419bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho GTPase-activating protein 35 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004916952.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGG CACGGAAACC AGATGTGCGT GTGCCCACCT ACAGTGTCAG CGTTGTGGGC 
CTTTCTGGCA CAGAGAAAGA GAAAGGCCAA TGTGGCGTTG GAAAATCATG CCTTTGTAAT 
CGATTTGTAA GACCCAGCGC AGATGATTTT CATCTGGACC ACACATCCGT CCTGAGCACT 
AGTGACTTTG GTGGTCGTGT TGTAAACAAT GACCATTTTC TGTACTGGGG AGAGAGTGGA 
CGGATGCCAG AAGACTGTGT AGACTTTAAA GTGCATGTGG TGGAGCAGAC AGAATTTATT 
GATGATCAGA CATTCCAGCC TCACCGTAGT ACTGCCCTAC AGCCTTACAT CAAGCGTGCT 
GCCTCCTGTA AGCTGGCCTC TGCTGAAAAA CTTATGTACT TCTGCACAGA TCAGTTGGGC 
TTGGAACAGG ACTTTGAGCA GAAGCAGATG CCTGAGGGTA AATTGTTGAT TGATGGTTTC 
CTGCTGTGTA TAGATGTTAG TCGTGGAATG AACCGGAACT TTGATGATCA GCTAAAATTT 
GTGTCCAATC TTTACACCCA GCTGGCCAAG ACAAAGAAAC CCATTGTTCT GGTTCTTACA 
AAATGTGATG AAGGAGTAGA ACGTTATATC CGCGATGCTC ATAGTTTTGC CCTTAACAAG 
AAGAACCTGC AAGTTGTAGA GACTTCTGCC CGGTCTAATG TGAATGTGGA TCTGGCCTTC 
ACTACACTAG TACAGCTTAT TGACAAAAGC CGTGGAAAGT CTAAAATTAT TCCTTATTTT 
GAGGCACTAA AGCAGCAGAG CCAACTGATT GCTGCCGCAA AGGACAAATA TGAATGGCTT 
GTGAGCCGAG TAGTAAAGTC TCATAATGAG ACATGGATCA ATCAAAGCAG GCGCATGCAG 
AGCAACATTG AATACCAAGA TTATGTGCAT TTGGAAGGAA CAGACAAAGC TAAGAAACTA 
TTCCATCAGC ACGTGCTACG GCTTAAACAG GAACACATAG AACGACGCAG AAGAAATTAC 
CTCTTAGCTC TCCCACGAGC CTTAGAAAGC CTTGTGCCAG ATCTGGATGA AATAGATCAC 
TTAAGCTGGG TTAAGGTTTC TAATTTGCTG GAATCAAAGG CAGAGTTCCT AAAATTTTTT 
ATTGTCCTGG AAGAGACACC TTGGGACTTC ACAATCCACA TTGACAGCAT TGATGAACGC 
ATACCTTATG ATTTGCTGGA AACAGTGCCT GCGGAAGAGG TCTATGCTGC CCACTTGGTC 
AAGTTAAGGA ATGCCAGGAA AAAGGAAGAA ATGAAGAGAT CTTTCAAGGA GAACTTGATC 
GCTTCTCCTT TCATTACACC AGGTAAGCCT TGGGAAGAGG CTCGGAGTTT TATCATGAGT 
GAAGAATTTT ACCAGTGGCT GGAAGAGCCT GTATATATGG AAATCTACAG CCGACACCAA 
AAAGAGATCA TTGATAAAGC TAAAGAGGAA TTTCAGGAGT TGTTGCTTGA GTATTCAGAG 
CTGTTTTATG AGCTAGAGTT AGATGCCAAA CCCAGTAAAG AGAAAATGGG CGTCATCCAG 
GATGTACTTG GTGAGGAACA ACGTTTCAAA GCATTACAGA AGCTGCAGGC GGAGCGAGAT 
GCCCTGATTC TTAAACACAT TCACTTTGTC TATCATCCAA CCAAGGACAC TTGTCCTAGC 
AACCCTTACT GTGTTGATAC AAGGATTGAG CATATACTCA GTTCCAGGTG CACATATCCC 
TATGACAGAC TACACAAAGA TCCAAATGTA GATAGGATCA ACCTTGTGAT CCTTGGAAAG 
GATGGTTTGG CACGAGAGTT AGCAAATGAG ATCCGAGCCC TCTGTACAAA TGATGATAAA 
TACGTAATTG ATGGAAAGAT GTATGAGTTA TCCCTAAGGC CTATAGAAGG CAATGTGCGG 
CTTCCTGTTA ATTCATTCCA GACATCCACT TTCCTCCCTC ATGGCTGTCT TTGCCTGTAC 
AACTCCAAGG AGTCTCTGTC CTATGTTGTG GAGAGCATTG AGAAAAGCAG AGAATCAAGC 
CTAGGTCGAA AGGAAAATCA CTTGATCCAT TTGCCTCTTA CGTTGATTCT TGTCAACAGG 
AGAGGAGACA CAAGTAGTGA AACTGCACAT AGCCTCATTC AACAGGGCAA ACAGGTTGCC 
AGTAAGCTTC AGTGTTTCTT TCTTGATGCA GCTTCCACTG GAATGGGATA TGGTCGCAAT 
ATCAATGAGA AGCAAATAAG CCTAATCCTC AGAGGGCTAC TGGAGTCAAA AAGAAACTTA 
AACCTTGTCA GCTCCACTCC AAGCATTAAA GATTTGACAG ATTGTGAGCT GCGTATTGTA 
ATGTGCCTCA TGTGTGCAGA CATATTTAAC ATAGAAGATG TGCTGAATGC ACTAAAACCT 
CACACTTACC GTCCATCACA ATGTGGGCTT GGTGGATCAG TTCTACTTGA CCTACCAATT 
GGCAGTCAAA AACGTCGCTT GGAGCTGCTG TTGTTGTCGT ATCATGCCTC CTTTAATGTT 
CGCAAGACTC GTTTAGTGCA CGGCTATATG ATTTTTTACG CAGCTAAGCG CCGTGCTTCC 
CTAGCTATGC TGCGTGCCTT TTTGTGTGAA GTGCAGGACA TTGTCCCTGT GCAGCTTGTT 
GCCTTGACTG AGGGACCTCT AACATTTTTG GATTATGTGG CACGAGAACA GTTACAAGAA 
GGGGAGGAAA TAGCTCAGGA CATTGAAGGT AAATTCCTTA TTCTACCACA AGGGCAGATA 
CAGCCAAAGT TGGATGTGTT TTACCCCTAC TTCAAAGATG TATTGGAGAA AAAAGTAATT 
ATTGAGGCCT CTCACATGTA TGATAATACA GCAGAGGCCT GCAGCACCAC AGAGGAAGTG 
TTTAGCTCAC CCCGTGCAGG CTCACCCCTT TGTAATTCCC ACTTGCCAGA CTCTGAAGAA 
GACCCTGAAC CTTCACCTCA ACACATTTTA TTTCAAAATG AACCTAACAT AGCAGCTCCC 
ATAAAATTTA CCAGAGAACT GGAGGAGACT GTGATGAACA CTTTAGAGAG CAAACTTAAC 
AACAAGGTAC CTCCTCCCCT TAAACCAAAG CCCCCTATTC CATATGAAAT TACCAAGCCT 
GAGCTATCAT ATTTGGATAC AGGGGCCCGG GATGGGCACC GTAAGTCCTT GACTTCCATA 
AACTGGCCCC CTTTGGAGGC CTTTGACCCT TCTGACTATG CTGAACCTAT GGATTCAGTA 
GTGAAGCCAA GAAACCAAGA GGAGAACATT TACTCTGTAC CCCATGATAG CACCCAAGGA 
AAGATCATCA CTATACGTAA TACCAACAAA CCACAATCTA ATGGGAGTGG CAATGGCTCA 
GACAGTGATA TGGACACAAA CTCATTGGAA CGAGCAAGAA AAGCATCCAT TGTCACTAAA 
CCTGTCCTTT ACCGTACCAA ATGTGCTAAA CTTGGGAAAT TCTCTAGCTA CAGGAGTAGT 
TTAAGTGTTG GCAGTGATGA TGAACCTGGA CCAATTCGAA GAAAGGAAGA GGAGACTGGA 
ACACAGGGTA CAAAGGGGGA CAGTACTACG AATTCTTATG AAGCTGATGG AGAGGACCCA 
CGCAAGAGGA ATATTTTGCG CAGTCTGCGA AGGACCACTA AGAAAGTGAA ACCCAAACCT 
CGTCCATCTT TAACCAAGGC GACCTGGGAG AGTAATTACT TTGGTTTCCC TCTGAGTAGT 
GTGGTGACTT CTGAACGACC AATTCCAGTA TTCATTGAGA AGTGTGTAGA GTACATCGAA 
GCCACGGGTG AGGGAATGAC CACAGAAGGC ATATACCGTG TTAGCGGGAA CAAATCTGAG 
ATGGAGTCTC TTCAAAGGCA ATTTGACCAA GATCATAACC TGGACCTGGC AGAAAAGGAT 
TTTACAGTGA ACACTGTGGC TGGAGCCTTG AAAAGTTTCT TCTCTGAATT ACCGGACCCT 
CTGGTGCCAT ACAATATGCA GGCTGAGCTG GTGGAGGCAT ATAAAATAAA TGATCTAGAA 
CATAAGCTCC AAGCTATGAA GGATCTTCTA AAAAAATTTC CAAAGGAAAA TCATGAAATC 
TTCAAATATG TTATATCTCA TTTAAACCGG GTTAGCCAGC ATCATCATAT AAATCTGATG 
ACCAGTGAAA ATTTATCTAT CTGCTTCTGG CCTACACTGA TGCGCCCTGA TTTTACCACT 
ATGGACGCTC TGACTGCAAC TCGCACATAC CAGACAATCA TTGAGCTTTT CATCCAACAG 
TGCCCCTTTT TCTTCTACCA GCGCCTTCCT GTGGACCTGC CTATCCCTAG TTCCCCATCT 
ACTCCACCTC TCACCCAGCC ATCTCCTCCT CAGTCCCCTC CTCTGACGCC TGTATCCCCC 
TCGCAGACCC TGCTTCCTAC GGAGCACAAC ATCCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_004917009.1
CDS	620..5038
Translation	MMMARKPDVRVPTYSVSVVGLSGTEKEKGQCGVGKSCLCNRFVRPSADDFHLDHTSVLSTSDFGGRVVNNDHFLYWGESGRMPEDCVDFKVHVVEQTEFIDDQTFQPHRSTALQPYIKRAASCKLASAEKLMYFCTDQLGLEQDFEQKQMPEGKLLIDGFLLCIDVSRGMNRNFDDQLKFVSNLYTQLAKTKKPIVLVLTKCDEGVERYIRDAHSFALNKKNLQVVETSARSNVNVDLAFTTLVQLIDKSRGKSKIIPYFEALKQQSQLIAAAKDKYEWLVSRVVKSHNETWINQSRRMQSNIEYQDYVHLEGTDKAKKLFHQHVLRLKQEHIERRRRNYLLALPRALESLVPDLDEIDHLSWVKVSNLLESKAEFLKFFIVLEETPWDFTIHIDSIDERIPYDLLETVPAEEVYAAHLVKLRNARKKEEMKRSFKENLIASPFITPGKPWEEARSFIMSEEFYQWLEEPVYMEIYSRHQKEIIDKAKEEFQELLLEYSELFYELELDAKPSKEKMGVIQDVLGEEQRFKALQKLQAERDALILKHIHFVYHPTKDTCPSNPYCVDTRIEHILSSRCTYPYDRLHKDPNVDRINLVILGKDGLARELANEIRALCTNDDKYVIDGKMYELSLRPIEGNVRLPVNSFQTSTFLPHGCLCLYNSKESLSYVVESIEKSRESSLGRKENHLIHLPLTLILVNRRGDTSSETAHSLIQQGKQVASKLQCFFLDAASTGMGYGRNINEKQISLILRGLLESKRNLNLVSSTPSIKDLTDCELRIVMCLMCADIFNIEDVLNALKPHTYRPSQCGLGGSVLLDLPIGSQKRRLELLLLSYHASFNVRKTRLVHGYMIFYAAKRRASLAMLRAFLCEVQDIVPVQLVALTEGPLTFLDYVAREQLQEGEEIAQDIEGKFLILPQGQIQPKLDVFYPYFKDVLEKKVIIEASHMYDNTAEACSTTEEVFSSPRAGSPLCNSHLPDSEEDPEPSPQHILFQNEPNIAAPIKFTRELEETVMNTLESKLNNKVPPPLKPKPPIPYEITKPELSYLDTGARDGHRKSLTSINWPPLEAFDPSDYAEPMDSVVKPRNQEENIYSVPHDSTQGKIITIRNTNKPQSNGSGNGSDSDMDTNSLERARKASIVTKPVLYRTKCAKLGKFSSYRSSLSVGSDDEPGPIRRKEEETGTQGTKGDSTTNSYEADGEDPRKRNILRSLRRTTKKVKPKPRPSLTKATWESNYFGFPLSSVVTSERPIPVFIEKCVEYIEATGEGMTTEGIYRVSGNKSEMESLQRQFDQDHNLDLAEKDFTVNTVAGALKSFFSELPDPLVPYNMQAELVEAYKINDLEHKLQAMKDLLKKFPKENHEIFKYVISHLNRVSQHHHINLMTSENLSICFWPTLMRPDFTTMDALTATRTYQTIIELFIQQCPFFFYQRLPVDLPIPSSPSTPPLTQPSPPQSPPLTPVSPSQTLLPTEHNIL

Target ORF information:

RefSeq Version	XM_004916952.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho GTPase activating protein 35 (arhgap35), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_004916952.3

ORF Insert Sequence:

ATGATGATGG CACGGAAACC AGATGTGCGT GTGCCCACCT ACAGTGTCAG CGTTGTGGGC 
CTTTCTGGCA CAGAGAAAGA GAAAGGCCAA TGTGGCGTTG GAAAATCATG CCTTTGTAAT 
CGATTTGTAA GACCCAGCGC AGATGATTTT CATCTGGACC ACACATCCGT CCTGAGCACT 
AGTGACTTTG GTGGTCGTGT TGTAAACAAT GACCATTTTC TGTACTGGGG AGAGAGTGGA 
CGGATGCCAG AAGACTGTGT AGACTTTAAA GTGCATGTGG TGGAGCAGAC AGAATTTATT 
GATGATCAGA CATTCCAGCC TCACCGTAGT ACTGCCCTAC AGCCTTACAT CAAGCGTGCT 
GCCTCCTGTA AGCTGGCCTC TGCTGAAAAA CTTATGTACT TCTGCACAGA TCAGTTGGGC 
TTGGAACAGG ACTTTGAGCA GAAGCAGATG CCTGAGGGTA AATTGTTGAT TGATGGTTTC 
CTGCTGTGTA TAGATGTTAG TCGTGGAATG AACCGGAACT TTGATGATCA GCTAAAATTT 
GTGTCCAATC TTTACACCCA GCTGGCCAAG ACAAAGAAAC CCATTGTTCT GGTTCTTACA 
AAATGTGATG AAGGAGTAGA ACGTTATATC CGCGATGCTC ATAGTTTTGC CCTTAACAAG 
AAGAACCTGC AAGTTGTAGA GACTTCTGCC CGGTCTAATG TGAATGTGGA TCTGGCCTTC 
ACTACACTAG TACAGCTTAT TGACAAAAGC CGTGGAAAGT CTAAAATTAT TCCTTATTTT 
GAGGCACTAA AGCAGCAGAG CCAACTGATT GCTGCCGCAA AGGACAAATA TGAATGGCTT 
GTGAGCCGAG TAGTAAAGTC TCATAATGAG ACATGGATCA ATCAAAGCAG GCGCATGCAG 
AGCAACATTG AATACCAAGA TTATGTGCAT TTGGAAGGAA CAGACAAAGC TAAGAAACTA 
TTCCATCAGC ACGTGCTACG GCTTAAACAG GAACACATAG AACGACGCAG AAGAAATTAC 
CTCTTAGCTC TCCCACGAGC CTTAGAAAGC CTTGTGCCAG ATCTGGATGA AATAGATCAC 
TTAAGCTGGG TTAAGGTTTC TAATTTGCTG GAATCAAAGG CAGAGTTCCT AAAATTTTTT 
ATTGTCCTGG AAGAGACACC TTGGGACTTC ACAATCCACA TTGACAGCAT TGATGAACGC 
ATACCTTATG ATTTGCTGGA AACAGTGCCT GCGGAAGAGG TCTATGCTGC CCACTTGGTC 
AAGTTAAGGA ATGCCAGGAA AAAGGAAGAA ATGAAGAGAT CTTTCAAGGA GAACTTGATC 
GCTTCTCCTT TCATTACACC AGGTAAGCCT TGGGAAGAGG CTCGGAGTTT TATCATGAGT 
GAAGAATTTT ACCAGTGGCT GGAAGAGCCT GTATATATGG AAATCTACAG CCGACACCAA 
AAAGAGATCA TTGATAAAGC TAAAGAGGAA TTTCAGGAGT TGTTGCTTGA GTATTCAGAG 
CTGTTTTATG AGCTAGAGTT AGATGCCAAA CCCAGTAAAG AGAAAATGGG CGTCATCCAG 
GATGTACTTG GTGAGGAACA ACGTTTCAAA GCATTACAGA AGCTGCAGGC GGAGCGAGAT 
GCCCTGATTC TTAAACACAT TCACTTTGTC TATCATCCAA CCAAGGACAC TTGTCCTAGC 
AACCCTTACT GTGTTGATAC AAGGATTGAG CATATACTCA GTTCCAGGTG CACATATCCC 
TATGACAGAC TACACAAAGA TCCAAATGTA GATAGGATCA ACCTTGTGAT CCTTGGAAAG 
GATGGTTTGG CACGAGAGTT AGCAAATGAG ATCCGAGCCC TCTGTACAAA TGATGATAAA 
TACGTAATTG ATGGAAAGAT GTATGAGTTA TCCCTAAGGC CTATAGAAGG CAATGTGCGG 
CTTCCTGTTA ATTCATTCCA GACATCCACT TTCCTCCCTC ATGGCTGTCT TTGCCTGTAC 
AACTCCAAGG AGTCTCTGTC CTATGTTGTG GAGAGCATTG AGAAAAGCAG AGAATCAAGC 
CTAGGTCGAA AGGAAAATCA CTTGATCCAT TTGCCTCTTA CGTTGATTCT TGTCAACAGG 
AGAGGAGACA CAAGTAGTGA AACTGCACAT AGCCTCATTC AACAGGGCAA ACAGGTTGCC 
AGTAAGCTTC AGTGTTTCTT TCTTGATGCA GCTTCCACTG GAATGGGATA TGGTCGCAAT 
ATCAATGAGA AGCAAATAAG CCTAATCCTC AGAGGGCTAC TGGAGTCAAA AAGAAACTTA 
AACCTTGTCA GCTCCACTCC AAGCATTAAA GATTTGACAG ATTGTGAGCT GCGTATTGTA 
ATGTGCCTCA TGTGTGCAGA CATATTTAAC ATAGAAGATG TGCTGAATGC ACTAAAACCT 
CACACTTACC GTCCATCACA ATGTGGGCTT GGTGGATCAG TTCTACTTGA CCTACCAATT 
GGCAGTCAAA AACGTCGCTT GGAGCTGCTG TTGTTGTCGT ATCATGCCTC CTTTAATGTT 
CGCAAGACTC GTTTAGTGCA CGGCTATATG ATTTTTTACG CAGCTAAGCG CCGTGCTTCC 
CTAGCTATGC TGCGTGCCTT TTTGTGTGAA GTGCAGGACA TTGTCCCTGT GCAGCTTGTT 
GCCTTGACTG AGGGACCTCT AACATTTTTG GATTATGTGG CACGAGAACA GTTACAAGAA 
GGGGAGGAAA TAGCTCAGGA CATTGAAGGT AAATTCCTTA TTCTACCACA AGGGCAGATA 
CAGCCAAAGT TGGATGTGTT TTACCCCTAC TTCAAAGATG TATTGGAGAA AAAAGTAATT 
ATTGAGGCCT CTCACATGTA TGATAATACA GCAGAGGCCT GCAGCACCAC AGAGGAAGTG 
TTTAGCTCAC CCCGTGCAGG CTCACCCCTT TGTAATTCCC ACTTGCCAGA CTCTGAAGAA 
GACCCTGAAC CTTCACCTCA ACACATTTTA TTTCAAAATG AACCTAACAT AGCAGCTCCC 
ATAAAATTTA CCAGAGAACT GGAGGAGACT GTGATGAACA CTTTAGAGAG CAAACTTAAC 
AACAAGGTAC CTCCTCCCCT TAAACCAAAG CCCCCTATTC CATATGAAAT TACCAAGCCT 
GAGCTATCAT ATTTGGATAC AGGGGCCCGG GATGGGCACC GTAAGTCCTT GACTTCCATA 
AACTGGCCCC CTTTGGAGGC CTTTGACCCT TCTGACTATG CTGAACCTAT GGATTCAGTA 
GTGAAGCCAA GAAACCAAGA GGAGAACATT TACTCTGTAC CCCATGATAG CACCCAAGGA 
AAGATCATCA CTATACGTAA TACCAACAAA CCACAATCTA ATGGGAGTGG CAATGGCTCA 
GACAGTGATA TGGACACAAA CTCATTGGAA CGAGCAAGAA AAGCATCCAT TGTCACTAAA 
CCTGTCCTTT ACCGTACCAA ATGTGCTAAA CTTGGGAAAT TCTCTAGCTA CAGGAGTAGT 
TTAAGTGTTG GCAGTGATGA TGAACCTGGA CCAATTCGAA GAAAGGAAGA GGAGACTGGA 
ACACAGGGTA CAAAGGGGGA CAGTACTACG AATTCTTATG AAGCTGATGG AGAGGACCCA 
CGCAAGAGGA ATATTTTGCG CAGTCTGCGA AGGACCACTA AGAAAGTGAA ACCCAAACCT 
CGTCCATCTT TAACCAAGGC GACCTGGGAG AGTAATTACT TTGGTTTCCC TCTGAGTAGT 
GTGGTGACTT CTGAACGACC AATTCCAGTA TTCATTGAGA AGTGTGTAGA GTACATCGAA 
GCCACGGGTG AGGGAATGAC CACAGAAGGC ATATACCGTG TTAGCGGGAA CAAATCTGAG 
ATGGAGTCTC TTCAAAGGCA ATTTGACCAA GATCATAACC TGGACCTGGC AGAAAAGGAT 
TTTACAGTGA ACACTGTGGC TGGAGCCTTG AAAAGTTTCT TCTCTGAATT ACCGGACCCT 
CTGGTGCCAT ACAATATGCA GGCTGAGCTG GTGGAGGCAT ATAAAATAAA TGATCTAGAA 
CATAAGCTCC AAGCTATGAA GGATCTTCTA AAAAAATTTC CAAAGGAAAA TCATGAAATC 
TTCAAATATG TTATATCTCA TTTAAACCGG GTTAGCCAGC ATCATCATAT AAATCTGATG 
ACCAGTGAAA ATTTATCTAT CTGCTTCTGG CCTACACTGA TGCGCCCTGA TTTTACCACT 
ATGGACGCTC TGACTGCAAC TCGCACATAC CAGACAATCA TTGAGCTTTT CATCCAACAG 
TGCCCCTTTT TCTTCTACCA GCGCCTTCCT GTGGACCTGC CTATCCCTAG TTCCCCATCT 
ACTCCACCTC TCACCCAGCC ATCTCCTCCT CAGTCCCCTC CTCTGACGCC TGTATCCCCC 
TCGCAGACCC TGCTTCCTAC GGAGCACAAC ATCCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgap35 ( XM_002932597.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002932643.1 Xenopus tropicalis Rho GTPase activating protein 35 (arhgap35), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OXa14507
Clone ID Related Accession (Same CDS sequence)	XM_002932597.4 , XM_002932597.5
Accession Version	XM_002932597.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4413bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	rho GTPase-activating protein 35 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002932597.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGG CACGGAAACC AGATGTGCGT GTGCCCACCT ACAGTGTCAG CGTTGTGGGC 
CTTTCTGGCA CAGAGAAAGA GAAAGGCCAA TGTGGCGTTG GAAAATCATG CCTTTGTAAT 
CGATTTGTAA GACCCAGCGC AGATGATTTT CATCTGGACC ACACATCCGT CCTGAGCACT 
AGTGACTTTG GTGGTCGTGT TGTAAACAAT GACCATTTTC TGTACTGGGG AGAGAGTGGA 
CGGATGCCAG AAGACTGTGT AGACTTTAAA GTGCATGTGG TGGAGCAGAC AGAATTTATT 
GATGATCAGA CATTCCAGCC TCACCGTAGT ACTGCCCTAC AGCCTTACAT CAAGCGTGCT 
GCCTCCTGTA AGCTGGCCTC TGCTGAAAAA CTTATGTACT TCTGCACAGA TCAGTTGGGC 
TTGGAACAGG ACTTTGAGCA GAAGCAGATG CCTGAGGGTA AATTGTTGAT TGATGGTTTC 
CTGCTGTGTA TAGATGTTAG TCGTGGAATG AACCGGAACT TTGATGATCA GCTAAAATTT 
GTGTCCAATC TTTACACCCA GCTGGCCAAG ACAAAGAAAC CCATTGTTCT GGTTCTTACA 
AAATGTGATG AAGGAGTAGA ACGTTATATC CGCGATGCTC ATAGTTTTGC CCTTAACAAG 
AAGAACCTGC AAGTTGTAGA GACTTCTGCC CGGTCTAATG TGAATGTGGA TCTGGCCTTC 
ACTACACTAG TACAGCTTAT TGACAAAAGC CGTGGAAAGT CTAAAATTAT TCCTTATTTT 
GAGGCACTAA AGCAGCAGAG CCAACTGATT GCTGCCGCAA AGGACAAATA TGAATGGCTT 
GTGAGCCGAG TAGTAAAGTC TCATAATGAG ACATGGATCA ATCAAAGCAG GCGCATGCAG 
AGCAACATTG AATACCAAGA TTATGTGCAT TTGGAAGGAA CAGACAAAGC TAAGAAACTA 
TTCCATCAGC ACGTGCTACG GCTTAAACAG GAACACATAG AACGACGCAG AAGAAATTAC 
CTCTTAGCTC TCCCACGAGC CTTAGAAAGC CTTGTGCCAG ATCTGGATGA AATAGATCAC 
TTAAGCTGGG TTAAGGTTTC TAATTTGCTG GAATCAAAGG CAGAGTTCCT AAAATTTTTT 
ATTGTCCTGG AAGAGACACC TTGGGACTTC ACAATCCACA TTGACAGCAT TGATGAACGC 
ATACCTTATG ATTTGCTGGA AACAGTGCCT GCGGAAGAGG TCTATGCTGC CCACTTGGTC 
AAGTTAAGGA ATGCCAGGAA AAAGGAAGAA ATGAAGAGAT CTTTCAAGGA GAACTTGATC 
GCTTCTCCTT TCATTACACC AGGTAAGCCT TGGGAAGAGG CTCGGAGTTT TATCATGAGT 
GAAGAATTTT ACCAGTGGCT GGAAGAGCCT GTATATATGG AAATCTACAG CCGACACCAA 
AAAGAGATCA TTGATAAAGC TAAAGAGGAA TTTCAGGAGT TGTTGCTTGA GTATTCAGAG 
CTGTTTTATG AGCTAGAGTT AGATGCCAAA CCCAGTAAAG AGAAAATGGG CGTCATCCAG 
GATGTACTTG GTGAGGAACA ACGTTTCAAA GCATTACAGA AGCTGCAGGC GGAGCGAGAT 
GCCCTGATTC TTAAACACAT TCACTTTGTC TATCATCCAA CCAAGGACAC TTGTCCTAGC 
AACCCTTACT GTGTTGATAC AAGGATTGAG CATATACTCA GTTCCAGGTG CACATATCCC 
TATGACAGAC TACACAAAGA TCCAAATGTA GATAGGATCA ACCTTGTGAT CCTTGGAAAG 
GATGGTTTGG CACGAGAGTT AGCAAATGAG ATCCGAGCCC TCTGTACAAA TGATGATAAA 
TACGTAATTG ATGGAAAGAT GTATGAGTTA TCCCTAAGGC CTATAGAAGG CAATGTGCGG 
CTTCCTGTTA ATTCATTCCA GACATCCACT TTCCTCCCTC ATGGCTGTCT TTGCCTGTAC 
AACTCCAAGG AGTCTCTGTC CTATGTTGTG GAGAGCATTG AGAAAAGCAG AGAATCAAGC 
CTAGGTCGAA AGGAAAATCA CTTGATCCAT TTGCCTCTTA CGTTGATTCT TGTCAACAGG 
AGAGGAGACA CAAGTAGTGA AACTGCACAT AGCCTCATTC AACAGGGCAA ACAGGTTGCC 
AGTAAGCTTC AGTGTTTCTT TCTTGATGCA GCTTCCACTG GAATGGGATA TGGTCGCAAT 
ATCAATGAGA AGCAAATAAG CCTAATCCTC AGAGGGCTAC TGGAGTCAAA AAGAAACTTA 
AACCTTGTCA GCTCCACTCC AAGCATTAAA GATTTGACAG ATTGTGAGCT GCGTATTGTA 
ATGTGCCTCA TGTGTGCAGA CATATTTAAC ATAGAAGATG TGCTGAATGC ACTAAAACCT 
CACACTTACC GTCCATCACA ATGTGGGCTT GGTGGATCAG TTCTACTTGA CCTACCAATT 
GGCAGTCAAA AACGTCGCTT GGAGCTGCTG TTGTTGTCGT ATCATGCCTC CTTTAATGTT 
CGCAAGACTC GTTTAGTGCA CGGCTATATG ATTTTTTACG CAGCTAAGCG CCGTGCTTCC 
CTAGCTATGC TGCGTGCCTT TTTGTGTGAA GTGCAGGACA TTGTCCCTGT GCAGCTTGTT 
GCCTTGACTG AGGGACCTCT AACATTTTTG GATTATGTGG CACGAGAACA GTTACAAGAA 
GGGGAGGAAA TAGCTCAGGA CATTGAAGGT AAATTCCTTA TTCTACCACA AGGGCAGATA 
CAGCCAAAGT TGGATGTGTT TTACCCCTAC TTCAAAGATG TATTGGAGAA AAAAGTAATT 
ATTGAGGCCT CTCACATGTA TGATAATACA GCAGAGGCCT GCAGCACCAC AGAGGAAGTG 
TTTAGCTCAC CCCGTGCAGG CTCACCCCTT TGTAATTCCC ACTTGCCAGA CTCTGAAGAA 
GACCCTGAAC CTTCACCTCA ACACATTTTA TTTCAAAATG AACCTAACAT AGCAGCTCCC 
ATAAAATTTA CCAGAGAACT GGAGGAGACT GTGATGAACA CTTTAGAGAG CAAACTTAAC 
AACAAGGTAC CTCCTCCCCT TAAACCAAAG CCCCCTATTC CATATGAAAT TACCAAGCCT 
GAGCTATCAT ATTTGGATAC AGGGGCCCGG GATGGGCACC GTAAGTCCTT GACTTCCATA 
AACTGGCCCC CTTTGGAGGC CTTTGACCCT TCTGACTATG CTGAACCTAT GGATTCAGTA 
GTGAAGCCAA GAAACCAAGA GGAGAACATT TACTCTGTAC CCCATGATAG CACCCAAGGA 
AAGATCATCA CTATACGTAA TACCAACAAA CCACAATCTA ATGGGAGTGG CAATGGCTCA 
GACAGTGATA TGGACACAAA CTCATTGGAA CGAGCAAGAA AAGCATCCAT TGTCACTAAA 
CCTGTCCTTT ACCGTACCAA ATGTGCTAAA CTTGGGAAAT TCTCTAGCTA CAGGAGTAGT 
TTAAGTGTTG GCAGTGATGA TGAACCTGGA CCAATTCGAA GAAAGGAAGA GGAGACTGGA 
ACACAGGGTA CAAAGGGGGA CAGTACTACG AATTCTTATG AAGCTGATGG AGAGGACCCA 
CGCAAGAGGA ATATTTTGCG CAGTCTGCGA AGGACCACTA AGAAAGTGAA ACCCAAACCT 
CGTCCATCTT TAACCAAGGC GACCTGGGAG AGTAATTACT TTGGTTTCCC TCTGAGTAGT 
GTGGTGACTT CTGAACGACC AATTCCAGTA TTCATTGAGA AGTGTGTAGA GTACATCGAA 
GCCACGGGAA TGACCACAGA AGGCATATAC CGTGTTAGCG GGAACAAATC TGAGATGGAG 
TCTCTTCAAA GGCAATTTGA CCAAGATCAT AACCTGGACC TGGCAGAAAA GGATTTTACA 
GTGAACACTG TGGCTGGAGC CTTGAAAAGT TTCTTCTCTG AATTACCGGA CCCTCTGGTG 
CCATACAATA TGCAGGCTGA GCTGGTGGAG GCATATAAAA TAAATGATCT AGAACATAAG 
CTCCAAGCTA TGAAGGATCT TCTAAAAAAA TTTCCAAAGG AAAATCATGA AATCTTCAAA 
TATGTTATAT CTCATTTAAA CCGGGTTAGC CAGCATCATC ATATAAATCT GATGACCAGT 
GAAAATTTAT CTATCTGCTT CTGGCCTACA CTGATGCGCC CTGATTTTAC CACTATGGAC 
GCTCTGACTG CAACTCGCAC ATACCAGACA ATCATTGAGC TTTTCATCCA ACAGTGCCCC 
TTTTTCTTCT ACCAGCGCCT TCCTGTGGAC CTGCCTATCC CTAGTTCCCC ATCTACTCCA 
CCTCTCACCC AGCCATCTCC TCCTCAGTCC CCTCCTCTGA CGCCTGTATC CCCCTCGCAG 
ACCCTGCTTC CTACGGAGCA CAACATCCTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002932643.1
CDS	560..4972
Translation	MMMARKPDVRVPTYSVSVVGLSGTEKEKGQCGVGKSCLCNRFVRPSADDFHLDHTSVLSTSDFGGRVVNNDHFLYWGESGRMPEDCVDFKVHVVEQTEFIDDQTFQPHRSTALQPYIKRAASCKLASAEKLMYFCTDQLGLEQDFEQKQMPEGKLLIDGFLLCIDVSRGMNRNFDDQLKFVSNLYTQLAKTKKPIVLVLTKCDEGVERYIRDAHSFALNKKNLQVVETSARSNVNVDLAFTTLVQLIDKSRGKSKIIPYFEALKQQSQLIAAAKDKYEWLVSRVVKSHNETWINQSRRMQSNIEYQDYVHLEGTDKAKKLFHQHVLRLKQEHIERRRRNYLLALPRALESLVPDLDEIDHLSWVKVSNLLESKAEFLKFFIVLEETPWDFTIHIDSIDERIPYDLLETVPAEEVYAAHLVKLRNARKKEEMKRSFKENLIASPFITPGKPWEEARSFIMSEEFYQWLEEPVYMEIYSRHQKEIIDKAKEEFQELLLEYSELFYELELDAKPSKEKMGVIQDVLGEEQRFKALQKLQAERDALILKHIHFVYHPTKDTCPSNPYCVDTRIEHILSSRCTYPYDRLHKDPNVDRINLVILGKDGLARELANEIRALCTNDDKYVIDGKMYELSLRPIEGNVRLPVNSFQTSTFLPHGCLCLYNSKESLSYVVESIEKSRESSLGRKENHLIHLPLTLILVNRRGDTSSETAHSLIQQGKQVASKLQCFFLDAASTGMGYGRNINEKQISLILRGLLESKRNLNLVSSTPSIKDLTDCELRIVMCLMCADIFNIEDVLNALKPHTYRPSQCGLGGSVLLDLPIGSQKRRLELLLLSYHASFNVRKTRLVHGYMIFYAAKRRASLAMLRAFLCEVQDIVPVQLVALTEGPLTFLDYVAREQLQEGEEIAQDIEGKFLILPQGQIQPKLDVFYPYFKDVLEKKVIIEASHMYDNTAEACSTTEEVFSSPRAGSPLCNSHLPDSEEDPEPSPQHILFQNEPNIAAPIKFTRELEETVMNTLESKLNNKVPPPLKPKPPIPYEITKPELSYLDTGARDGHRKSLTSINWPPLEAFDPSDYAEPMDSVVKPRNQEENIYSVPHDSTQGKIITIRNTNKPQSNGSGNGSDSDMDTNSLERARKASIVTKPVLYRTKCAKLGKFSSYRSSLSVGSDDEPGPIRRKEEETGTQGTKGDSTTNSYEADGEDPRKRNILRSLRRTTKKVKPKPRPSLTKATWESNYFGFPLSSVVTSERPIPVFIEKCVEYIEATGMTTEGIYRVSGNKSEMESLQRQFDQDHNLDLAEKDFTVNTVAGALKSFFSELPDPLVPYNMQAELVEAYKINDLEHKLQAMKDLLKKFPKENHEIFKYVISHLNRVSQHHHINLMTSENLSICFWPTLMRPDFTTMDALTATRTYQTIIELFIQQCPFFFYQRLPVDLPIPSSPSTPPLTQPSPPQSPPLTPVSPSQTLLPTEHNIL

Target ORF information:

RefSeq Version	XM_002932597.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis Rho GTPase activating protein 35 (arhgap35), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002932597.4

ORF Insert Sequence:

ATGATGATGG CACGGAAACC AGATGTGCGT GTGCCCACCT ACAGTGTCAG CGTTGTGGGC 
CTTTCTGGCA CAGAGAAAGA GAAAGGCCAA TGTGGCGTTG GAAAATCATG CCTTTGTAAT 
CGATTTGTAA GACCCAGCGC AGATGATTTT CATCTGGACC ACACATCCGT CCTGAGCACT 
AGTGACTTTG GTGGTCGTGT TGTAAACAAT GACCATTTTC TGTACTGGGG AGAGAGTGGA 
CGGATGCCAG AAGACTGTGT AGACTTTAAA GTGCATGTGG TGGAGCAGAC AGAATTTATT 
GATGATCAGA CATTCCAGCC TCACCGTAGT ACTGCCCTAC AGCCTTACAT CAAGCGTGCT 
GCCTCCTGTA AGCTGGCCTC TGCTGAAAAA CTTATGTACT TCTGCACAGA TCAGTTGGGC 
TTGGAACAGG ACTTTGAGCA GAAGCAGATG CCTGAGGGTA AATTGTTGAT TGATGGTTTC 
CTGCTGTGTA TAGATGTTAG TCGTGGAATG AACCGGAACT TTGATGATCA GCTAAAATTT 
GTGTCCAATC TTTACACCCA GCTGGCCAAG ACAAAGAAAC CCATTGTTCT GGTTCTTACA 
AAATGTGATG AAGGAGTAGA ACGTTATATC CGCGATGCTC ATAGTTTTGC CCTTAACAAG 
AAGAACCTGC AAGTTGTAGA GACTTCTGCC CGGTCTAATG TGAATGTGGA TCTGGCCTTC 
ACTACACTAG TACAGCTTAT TGACAAAAGC CGTGGAAAGT CTAAAATTAT TCCTTATTTT 
GAGGCACTAA AGCAGCAGAG CCAACTGATT GCTGCCGCAA AGGACAAATA TGAATGGCTT 
GTGAGCCGAG TAGTAAAGTC TCATAATGAG ACATGGATCA ATCAAAGCAG GCGCATGCAG 
AGCAACATTG AATACCAAGA TTATGTGCAT TTGGAAGGAA CAGACAAAGC TAAGAAACTA 
TTCCATCAGC ACGTGCTACG GCTTAAACAG GAACACATAG AACGACGCAG AAGAAATTAC 
CTCTTAGCTC TCCCACGAGC CTTAGAAAGC CTTGTGCCAG ATCTGGATGA AATAGATCAC 
TTAAGCTGGG TTAAGGTTTC TAATTTGCTG GAATCAAAGG CAGAGTTCCT AAAATTTTTT 
ATTGTCCTGG AAGAGACACC TTGGGACTTC ACAATCCACA TTGACAGCAT TGATGAACGC 
ATACCTTATG ATTTGCTGGA AACAGTGCCT GCGGAAGAGG TCTATGCTGC CCACTTGGTC 
AAGTTAAGGA ATGCCAGGAA AAAGGAAGAA ATGAAGAGAT CTTTCAAGGA GAACTTGATC 
GCTTCTCCTT TCATTACACC AGGTAAGCCT TGGGAAGAGG CTCGGAGTTT TATCATGAGT 
GAAGAATTTT ACCAGTGGCT GGAAGAGCCT GTATATATGG AAATCTACAG CCGACACCAA 
AAAGAGATCA TTGATAAAGC TAAAGAGGAA TTTCAGGAGT TGTTGCTTGA GTATTCAGAG 
CTGTTTTATG AGCTAGAGTT AGATGCCAAA CCCAGTAAAG AGAAAATGGG CGTCATCCAG 
GATGTACTTG GTGAGGAACA ACGTTTCAAA GCATTACAGA AGCTGCAGGC GGAGCGAGAT 
GCCCTGATTC TTAAACACAT TCACTTTGTC TATCATCCAA CCAAGGACAC TTGTCCTAGC 
AACCCTTACT GTGTTGATAC AAGGATTGAG CATATACTCA GTTCCAGGTG CACATATCCC 
TATGACAGAC TACACAAAGA TCCAAATGTA GATAGGATCA ACCTTGTGAT CCTTGGAAAG 
GATGGTTTGG CACGAGAGTT AGCAAATGAG ATCCGAGCCC TCTGTACAAA TGATGATAAA 
TACGTAATTG ATGGAAAGAT GTATGAGTTA TCCCTAAGGC CTATAGAAGG CAATGTGCGG 
CTTCCTGTTA ATTCATTCCA GACATCCACT TTCCTCCCTC ATGGCTGTCT TTGCCTGTAC 
AACTCCAAGG AGTCTCTGTC CTATGTTGTG GAGAGCATTG AGAAAAGCAG AGAATCAAGC 
CTAGGTCGAA AGGAAAATCA CTTGATCCAT TTGCCTCTTA CGTTGATTCT TGTCAACAGG 
AGAGGAGACA CAAGTAGTGA AACTGCACAT AGCCTCATTC AACAGGGCAA ACAGGTTGCC 
AGTAAGCTTC AGTGTTTCTT TCTTGATGCA GCTTCCACTG GAATGGGATA TGGTCGCAAT 
ATCAATGAGA AGCAAATAAG CCTAATCCTC AGAGGGCTAC TGGAGTCAAA AAGAAACTTA 
AACCTTGTCA GCTCCACTCC AAGCATTAAA GATTTGACAG ATTGTGAGCT GCGTATTGTA 
ATGTGCCTCA TGTGTGCAGA CATATTTAAC ATAGAAGATG TGCTGAATGC ACTAAAACCT 
CACACTTACC GTCCATCACA ATGTGGGCTT GGTGGATCAG TTCTACTTGA CCTACCAATT 
GGCAGTCAAA AACGTCGCTT GGAGCTGCTG TTGTTGTCGT ATCATGCCTC CTTTAATGTT 
CGCAAGACTC GTTTAGTGCA CGGCTATATG ATTTTTTACG CAGCTAAGCG CCGTGCTTCC 
CTAGCTATGC TGCGTGCCTT TTTGTGTGAA GTGCAGGACA TTGTCCCTGT GCAGCTTGTT 
GCCTTGACTG AGGGACCTCT AACATTTTTG GATTATGTGG CACGAGAACA GTTACAAGAA 
GGGGAGGAAA TAGCTCAGGA CATTGAAGGT AAATTCCTTA TTCTACCACA AGGGCAGATA 
CAGCCAAAGT TGGATGTGTT TTACCCCTAC TTCAAAGATG TATTGGAGAA AAAAGTAATT 
ATTGAGGCCT CTCACATGTA TGATAATACA GCAGAGGCCT GCAGCACCAC AGAGGAAGTG 
TTTAGCTCAC CCCGTGCAGG CTCACCCCTT TGTAATTCCC ACTTGCCAGA CTCTGAAGAA 
GACCCTGAAC CTTCACCTCA ACACATTTTA TTTCAAAATG AACCTAACAT AGCAGCTCCC 
ATAAAATTTA CCAGAGAACT GGAGGAGACT GTGATGAACA CTTTAGAGAG CAAACTTAAC 
AACAAGGTAC CTCCTCCCCT TAAACCAAAG CCCCCTATTC CATATGAAAT TACCAAGCCT 
GAGCTATCAT ATTTGGATAC AGGGGCCCGG GATGGGCACC GTAAGTCCTT GACTTCCATA 
AACTGGCCCC CTTTGGAGGC CTTTGACCCT TCTGACTATG CTGAACCTAT GGATTCAGTA 
GTGAAGCCAA GAAACCAAGA GGAGAACATT TACTCTGTAC CCCATGATAG CACCCAAGGA 
AAGATCATCA CTATACGTAA TACCAACAAA CCACAATCTA ATGGGAGTGG CAATGGCTCA 
GACAGTGATA TGGACACAAA CTCATTGGAA CGAGCAAGAA AAGCATCCAT TGTCACTAAA 
CCTGTCCTTT ACCGTACCAA ATGTGCTAAA CTTGGGAAAT TCTCTAGCTA CAGGAGTAGT 
TTAAGTGTTG GCAGTGATGA TGAACCTGGA CCAATTCGAA GAAAGGAAGA GGAGACTGGA 
ACACAGGGTA CAAAGGGGGA CAGTACTACG AATTCTTATG AAGCTGATGG AGAGGACCCA 
CGCAAGAGGA ATATTTTGCG CAGTCTGCGA AGGACCACTA AGAAAGTGAA ACCCAAACCT 
CGTCCATCTT TAACCAAGGC GACCTGGGAG AGTAATTACT TTGGTTTCCC TCTGAGTAGT 
GTGGTGACTT CTGAACGACC AATTCCAGTA TTCATTGAGA AGTGTGTAGA GTACATCGAA 
GCCACGGGAA TGACCACAGA AGGCATATAC CGTGTTAGCG GGAACAAATC TGAGATGGAG 
TCTCTTCAAA GGCAATTTGA CCAAGATCAT AACCTGGACC TGGCAGAAAA GGATTTTACA 
GTGAACACTG TGGCTGGAGC CTTGAAAAGT TTCTTCTCTG AATTACCGGA CCCTCTGGTG 
CCATACAATA TGCAGGCTGA GCTGGTGGAG GCATATAAAA TAAATGATCT AGAACATAAG 
CTCCAAGCTA TGAAGGATCT TCTAAAAAAA TTTCCAAAGG AAAATCATGA AATCTTCAAA 
TATGTTATAT CTCATTTAAA CCGGGTTAGC CAGCATCATC ATATAAATCT GATGACCAGT 
GAAAATTTAT CTATCTGCTT CTGGCCTACA CTGATGCGCC CTGATTTTAC CACTATGGAC 
GCTCTGACTG CAACTCGCAC ATACCAGACA ATCATTGAGC TTTTCATCCA ACAGTGCCCC 
TTTTTCTTCT ACCAGCGCCT TCCTGTGGAC CTGCCTATCC CTAGTTCCCC ATCTACTCCA 
CCTCTCACCC AGCCATCTCC TCCTCAGTCC CCTCCTCTGA CGCCTGTATC CCCCTCGCAG 
ACCCTGCTTC CTACGGAGCA CAACATCCTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	arhgap35
Entrez Gene ID	100498460
Full Name	Rho GTPase activating protein 35
Synonyms	grf-1,grlf1,p190-a,p190a,p190arhogap,p190rhogap
General protein information	Preferred Names Rho GTPase activating protein 35 Names rho GTPase-activating protein 35
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

Pan troglodytes (chimpanzee)	ARHGAP35	XP_003316510.1
	GRLF1	XP_001112719.1
Rattus norvegicus (Norway rat)	Arhgap35	NP_001258061.1
Gallus gallus (chicken)	ARHGAP35	XP_003643808.2
Homo sapiens (human)	ARHGAP35	NP_004482.4
Bos taurus (cattle)	ARHGAP35	NP_001179327.1
Danio rerio (zebrafish)	arhgap35	XP_003198691.2
Danio rerio (zebrafish)	LOC100004299	XP_001343636.1
Xenopus tropicalis (tropical clawed frog)	arhgap35	XP_004917009.1
Drosophila melanogaster (fruit fly)	RhoGAPp190	NP_001259658.1
Mus musculus (house mouse)	Grlf1	NP_766327.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000870	XP_316844.5
Canis lupus familiaris (dog)	ARHGAP35	NP_001003022.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

arhgap35 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

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