Tmtc2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tmtc2
Entrez Gene ID	299762
Full Name	transmembrane and tetratricopeptide repeat containing 2
Synonyms	RGD1309848
General protein information	Preferred Names transmembrane and tetratricopeptide repeat containing 2 Names transmembrane and TPR repeat-containing protein 2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q21

mRNA and Protein(s)

mRNA	Protein	Name
NM_001171177.2	NP_001164648.2	transmembrane and TPR repeat-containing protein 2 precursor
XM_017594739.1	XP_017450228.1	transmembrane and TPR repeat-containing protein 2 isoform X1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	tmtc2	NP_001124075.1
Pan troglodytes (chimpanzee)	TMTC2	XP_003313880.1
Canis lupus familiaris (dog)	TMTC2	XP_539701.3
	TMTC2	XP_001086436.1
Bos taurus (cattle)	TMTC2	XP_005206142.1
Rattus norvegicus (Norway rat)	Tmtc2	NP_001164648.2
Homo sapiens (human)	TMTC2	NP_689801.1
Mus musculus (house mouse)	Tmtc2	NP_796342.2
Gallus gallus (chicken)	TMTC2	XP_416123.3
Drosophila melanogaster (fruit fly)	CG4341	NP_608558.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009112	XP_319863.4
Caenorhabditis elegans (roundworm)	F32D1.3	NP_504200.1
Xenopus tropicalis (tropical clawed frog)	tmtc2	NP_001096156.1

Gene Ontology
Bibliography

Related articles in PubMed

Discovery of an O-mannosylation pathway selectively serving cadherins and protocadherins.
Larsen ISB, Narimatsu Y, Joshi HJ, Siukstaite L, Harrison OJ, Brasch J, Goodman KM, Hansen L, Shapiro L, Honig B, Vakhrushev SY, Clausen H, Halim A
Proceedings of the National Academy of Sciences of the United States of America114(42)11163-11168(2017 Oct)

Association of TMTC2 With Human Nonsyndromic Sensorineural Hearing Loss.
Runge CL, Indap A, Zhou Y, Kent JW Jr, King E, Erbe CB, Cole R, Littrell J, Merath K, James R, R?schendorf F, Kerschner JE, Marth G, H?bner N, G?ring HH, Friedland DR, Kwok WM, Olivier M
JAMA otolaryngology-- head & neck surgery142(9)866-72(2016 Sep)

TMTC1 and TMTC2 are novel endoplasmic reticulum tetratricopeptide repeat-containing adapter proteins involved in calcium homeostasis.
Sunryd JC, Cheon B, Graham JB, Giorda KM, Fissore RA, Hebert DN
The Journal of biological chemistry289(23)16085-99(2014 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Tmtc2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tmtc2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa06407	NM_001171177.2 Latest version!	Rattus norvegicus transmembrane O-mannosyltransferase targeting cadherins 2 (Tmtc2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa61941	XM_017594739.1 Latest version!	Rattus norvegicus transmembrane and tetratricopeptide repeat containing 2 (Tmtc2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tmtc2 ( NM_001171177.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001164648.2 Rattus norvegicus transmembrane O-mannosyltransferase targeting cadherins 2 (Tmtc2), mRNA.

Price & Availability↑

CloneID	ORa06407
Clone ID Related Accession (Same CDS sequence)	NM_001171177.2
Accession Version	NM_001171177.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2511bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-12
Organism	Rattus norvegicus(Norway rat)
Product	transmembrane and TPR repeat-containing protein 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAHX01048936.1, AAHX01048923.1, AAHX01048922.1, AAHX01048918.1, AAHX01048916.1, AAHX01048915.1 and AAHX01048913.1. On or before Apr 24, 2011 this sequence version replaced XM_235136.5, XM_001080732.2, NM_001171177.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGATTGCAG AGTTGGTGAG CAGCGCTCTG GGGCTCGCCT TGTATCTCAA CACCCTGAGT 
GCGGATTTCT GCTACGATGA CAGCCGTGCT ATCAAGACTA ATCAGGACCT GCTGCCAGAA 
ACCCCCTGGA CCCACATTTT CTATAATGAT TTCTGGGGGA CTCTTCTAAC CCACAGTGGT 
AGCCACAAAT CCTACCGGCC GCTCTGCACT CTTTCTTTTC GACTGAATCA TGCCATCGGA 
GGGCTGAATC CCTGGAGTTA TCACCTTGTC AATGTCCTGT TGCACGCGGC CGTCACCGGC 
CTCTTCACAC GCTTCTCCAA GGCCCTGCTT GGGGACGGAT ACTGGACTTT CATGGCGGGC 
TTGATGTTTG CCTCGCACCC CATTCACACG GAGGCAGTGG CGGGCATCGT GGGCCGGGCA 
GACGTGGGCG CCAGTCTCTT TTTCCTCCTG TCCTTGCTCT GCTACACCAA GCACTGTTCT 
ACAAGAGGCT ACTCAGCCAG AACTTGGGGC TGGTTCCTGG GGACAGGACT GTGCGCTGGA 
TGCAGCATGC TGTGGAAGGA GCAAGGAGTG ACCGTCCTCG CAGTTTCAGC CGTTTATGAC 
GTCTTTGTCT TCCACAGGCT GAAGATGAGG CAGATTCTTC CTACCATTTA CAAAAGGAAG 
AACTTGTCTC TCTTTCTCAG CATTAGCTTG TTAACATTCT GGGGGACCAG CCTTTTGGGT 
GCCCGGTTAT ACTGGATGGG CAATAAACCA CCAAGCTTTT CCAACTCCGA CAACCCCGCT 
GCTGACTCGG ACAGCCTTTT GGCTCGCACG CTCACTTTCT TCTATTTACC TACCAAGAAC 
CTCTGGCTAT TGCTCTGCCC GGACACCCTG AGCTTTGATT GGTCCATGGA CGCCGTGCCT 
CTTCTCAAAA CAGTTTGTGA TTGGAGAAAT CTACACACTG TGGCCTTCTA TTCTGGACTT 
CTCCTGCTCG CCTACTGTGG TTTGAAGAGC CCAAGTTTAG AAGGAGAGTG CAACGGGAAA 
GCTGTGACAA ACGGCAAGCA GAACGCCAAT GGCCATAGTT GCTACTCAGA CGTGGAGTAC 
CGGAACTCAG AAATTAAGCC CAGCTTTGCA TCCAAAGTAG AGAATGGAAT TAAAAGTTGC 
GTATCACAGA GACCTCAGCT CCCTTCCACT GAGAACATTG TCATCCTGTC TTTATCTTTG 
TTGATAATAC CTTTTATTCC TGCCACGAAC CTGTTTTTCT ATGTCGGCTT TGTAATCGCA 
GAGCGAGTCT TATATATTCC CAGTATGGGC TTCTGCCTTC TGATCACAGT GGGCGCCAGA 
GCCCTTTATG TCAAAGTACA AAAGAGGTTT CTCAAGAGCC TGGTTTTTTA TGCTACAGCA 
ACGTTAATTG TCTTCTACGG ACTCAAGACT GCGATCAGGA ACGGAGACTG GCAGAATGAG 
GAGATGCTGT ATAGATCAGG GATCAAAGTG AACCCCGCCA AAGCATGGGG TAACCTTGGA 
AATGTTCTGA AGAGTCAGAG TAAAATCTCT GAAGCTGAAA TCGCCTATAG AAATGCTCTG 
TACTACCGCA GCAACATGGC TGACATGCTG TATAATTTAG GGTTGCTGCT CCAAGAGAAC 
AGCAGGTTTG CAGAAGCTCT CCATTATTAC AAACTGGCTG TCGGCAGCAG GCCTACCCTG 
GCTTCTGCCT ATTTAAACAC CGGCATCATT CTCATGAACC AAGGGAAGAC AGAAGAAGCC 
CGACGTACAT TCCTCAAGTG TTCTGAGATT CCAGATGAGA ATCTGAAGGA TCCTCATGCA 
CATAAGAGTT CTGTTACCAG CTGCCTGTAC AACCTCGGGA AACTCTATCA TGAGCAAGGA 
CGCTATGAGG AAGCCCTGAG TGTTTATAGG GAAGCAATTC AGAAGATGCC AAGGCAGTTT 
GCCCCTCAGA GCTTATACAA CATGATGGGC GAAGCCTACA TGAGACTGAG TAAGCTTCCT 
GAAGCTGAGC ATTGGTACAT GGAATCATTG AGATCCAAGA CGGACCACAT CCCTGCTCAT 
CTTACCTATG GGAAGCTGCT GGCGCTAACA GGTCGTAAGA GTGAGGCTGA AAAGTTCTTC 
TTGAAGGCTA TTGAGCTGGA TCCTACCAAA GGAAACTGTT ACATGCACTA TGGTCAGTTT 
CTCCTGGAGG AATCTCGCCT CACAGAAGCA GCCGAGATGG CAAAAAAAGC AGCTGAGCTG 
GACAGCACAG AGTTCGATGT CGTCTTCAAT GCTGCCCACA TGCTCAGACA AGCTAGCCTC 
AATGAAGCTG CGGAGAAGTA TTACGATCTT GCAGCAAGAC TGAGACCTAA TTACCCTGCT 
GCTCTCATGA ACCTAGGGGC CATCCTACAT CTCAATGGGC GACTCCAGAA GGCTGAGGCC 
AACTACCTGA GGGCTCTGCA GCTCAAGCCG GATGATGTCA TCACTCAGTC CAATCTCCGC 
AAGCTGTGGA ACATCATGGA AAAGCAAGGC TTGAAGACCT CCAAGACCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001164648.2
CDS	1..2511
Translation	MIAELVSSALGLALYLNTLSADFCYDDSRAIKTNQDLLPETPWTHIFYNDFWGTLLTHSGSHKSYRPLCTLSFRLNHAIGGLNPWSYHLVNVLLHAAVTGLFTRFSKALLGDGYWTFMAGLMFASHPIHTEAVAGIVGRADVGASLFFLLSLLCYTKHCSTRGYSARTWGWFLGTGLCAGCSMLWKEQGVTVLAVSAVYDVFVFHRLKMRQILPTIYKRKNLSLFLSISLLTFWGTSLLGARLYWMGNKPPSFSNSDNPAADSDSLLARTLTFFYLPTKNLWLLLCPDTLSFDWSMDAVPLLKTVCDWRNLHTVAFYSGLLLLAYCGLKSPSLEGECNGKAVTNGKQNANGHSCYSDVEYRNSEIKPSFASKVENGIKSCVSQRPQLPSTENIVILSLSLLIIPFIPATNLFFYVGFVIAERVLYIPSMGFCLLITVGARALYVKVQKRFLKSLVFYATATLIVFYGLKTAIRNGDWQNEEMLYRSGIKVNPAKAWGNLGNVLKSQSKISEAEIAYRNALYYRSNMADMLYNLGLLLQENSRFAEALHYYKLAVGSRPTLASAYLNTGIILMNQGKTEEARRTFLKCSEIPDENLKDPHAHKSSVTSCLYNLGKLYHEQGRYEEALSVYREAIQKMPRQFAPQSLYNMMGEAYMRLSKLPEAEHWYMESLRSKTDHIPAHLTYGKLLALTGRKSEAEKFFLKAIELDPTKGNCYMHYGQFLLEESRLTEAAEMAKKAAELDSTEFDVVFNAAHMLRQASLNEAAEKYYDLAARLRPNYPAALMNLGAILHLNGRLQKAEANYLRALQLKPDDVITQSNLRKLWNIMEKQGLKTSKT

Target ORF information:

RefSeq Version	NM_001171177.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus transmembrane O-mannosyltransferase targeting cadherins 2 (Tmtc2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001171177.2

ORF Insert Sequence:

ATGATTGCAG AGTTGGTGAG CAGCGCTCTG GGGCTCGCCT TGTATCTCAA CACCCTGAGT 
GCGGATTTCT GCTACGATGA CAGCCGTGCT ATCAAGACTA ATCAGGACCT GCTGCCAGAA 
ACCCCCTGGA CCCACATTTT CTATAATGAT TTCTGGGGGA CTCTTCTAAC CCACAGTGGT 
AGCCACAAAT CCTACCGGCC GCTCTGCACT CTTTCTTTTC GACTGAATCA TGCCATCGGA 
GGGCTGAATC CCTGGAGTTA TCACCTTGTC AATGTCCTGT TGCACGCGGC CGTCACCGGC 
CTCTTCACAC GCTTCTCCAA GGCCCTGCTT GGGGACGGAT ACTGGACTTT CATGGCGGGC 
TTGATGTTTG CCTCGCACCC CATTCACACG GAGGCAGTGG CGGGCATCGT GGGCCGGGCA 
GACGTGGGCG CCAGTCTCTT TTTCCTCCTG TCCTTGCTCT GCTACACCAA GCACTGTTCT 
ACAAGAGGCT ACTCAGCCAG AACTTGGGGC TGGTTCCTGG GGACAGGACT GTGCGCTGGA 
TGCAGCATGC TGTGGAAGGA GCAAGGAGTG ACCGTCCTCG CAGTTTCAGC CGTTTATGAC 
GTCTTTGTCT TCCACAGGCT GAAGATGAGG CAGATTCTTC CTACCATTTA CAAAAGGAAG 
AACTTGTCTC TCTTTCTCAG CATTAGCTTG TTAACATTCT GGGGGACCAG CCTTTTGGGT 
GCCCGGTTAT ACTGGATGGG CAATAAACCA CCAAGCTTTT CCAACTCCGA CAACCCCGCT 
GCTGACTCGG ACAGCCTTTT GGCTCGCACG CTCACTTTCT TCTATTTACC TACCAAGAAC 
CTCTGGCTAT TGCTCTGCCC GGACACCCTG AGCTTTGATT GGTCCATGGA CGCCGTGCCT 
CTTCTCAAAA CAGTTTGTGA TTGGAGAAAT CTACACACTG TGGCCTTCTA TTCTGGACTT 
CTCCTGCTCG CCTACTGTGG TTTGAAGAGC CCAAGTTTAG AAGGAGAGTG CAACGGGAAA 
GCTGTGACAA ACGGCAAGCA GAACGCCAAT GGCCATAGTT GCTACTCAGA CGTGGAGTAC 
CGGAACTCAG AAATTAAGCC CAGCTTTGCA TCCAAAGTAG AGAATGGAAT TAAAAGTTGC 
GTATCACAGA GACCTCAGCT CCCTTCCACT GAGAACATTG TCATCCTGTC TTTATCTTTG 
TTGATAATAC CTTTTATTCC TGCCACGAAC CTGTTTTTCT ATGTCGGCTT TGTAATCGCA 
GAGCGAGTCT TATATATTCC CAGTATGGGC TTCTGCCTTC TGATCACAGT GGGCGCCAGA 
GCCCTTTATG TCAAAGTACA AAAGAGGTTT CTCAAGAGCC TGGTTTTTTA TGCTACAGCA 
ACGTTAATTG TCTTCTACGG ACTCAAGACT GCGATCAGGA ACGGAGACTG GCAGAATGAG 
GAGATGCTGT ATAGATCAGG GATCAAAGTG AACCCCGCCA AAGCATGGGG TAACCTTGGA 
AATGTTCTGA AGAGTCAGAG TAAAATCTCT GAAGCTGAAA TCGCCTATAG AAATGCTCTG 
TACTACCGCA GCAACATGGC TGACATGCTG TATAATTTAG GGTTGCTGCT CCAAGAGAAC 
AGCAGGTTTG CAGAAGCTCT CCATTATTAC AAACTGGCTG TCGGCAGCAG GCCTACCCTG 
GCTTCTGCCT ATTTAAACAC CGGCATCATT CTCATGAACC AAGGGAAGAC AGAAGAAGCC 
CGACGTACAT TCCTCAAGTG TTCTGAGATT CCAGATGAGA ATCTGAAGGA TCCTCATGCA 
CATAAGAGTT CTGTTACCAG CTGCCTGTAC AACCTCGGGA AACTCTATCA TGAGCAAGGA 
CGCTATGAGG AAGCCCTGAG TGTTTATAGG GAAGCAATTC AGAAGATGCC AAGGCAGTTT 
GCCCCTCAGA GCTTATACAA CATGATGGGC GAAGCCTACA TGAGACTGAG TAAGCTTCCT 
GAAGCTGAGC ATTGGTACAT GGAATCATTG AGATCCAAGA CGGACCACAT CCCTGCTCAT 
CTTACCTATG GGAAGCTGCT GGCGCTAACA GGTCGTAAGA GTGAGGCTGA AAAGTTCTTC 
TTGAAGGCTA TTGAGCTGGA TCCTACCAAA GGAAACTGTT ACATGCACTA TGGTCAGTTT 
CTCCTGGAGG AATCTCGCCT CACAGAAGCA GCCGAGATGG CAAAAAAAGC AGCTGAGCTG 
GACAGCACAG AGTTCGATGT CGTCTTCAAT GCTGCCCACA TGCTCAGACA AGCTAGCCTC 
AATGAAGCTG CGGAGAAGTA TTACGATCTT GCAGCAAGAC TGAGACCTAA TTACCCTGCT 
GCTCTCATGA ACCTAGGGGC CATCCTACAT CTCAATGGGC GACTCCAGAA GGCTGAGGCC 
AACTACCTGA GGGCTCTGCA GCTCAAGCCG GATGATGTCA TCACTCAGTC CAATCTCCGC 
AAGCTGTGGA ACATCATGGA AAAGCAAGGC TTGAAGACCT CCAAGACCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tmtc2 ( XM_017594739.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017450228.1 Rattus norvegicus transmembrane and tetratricopeptide repeat containing 2 (Tmtc2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa61941
Clone ID Related Accession (Same CDS sequence)	XM_017594739.1
Accession Version	XM_017594739.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2322bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	transmembrane and TPR repeat-containing protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) and transcript sequence (NM_001171177.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 50 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGATTGCAG AGTTGGTGAG CAGCGCTCTG GGGCTCGCCT TGTATCTCAA CACCCTGAGT 
GCGGATTTCT GCTACGATGA CAGCCGTGCT ATCAAGACTA ATCAGGACCT GCTGCCAGAA 
ACCCCCTGGA CCCACATTTT CTATAATGAT TTCTGGGGGA CTCTTCTAAC CCACAGTGGT 
AGCCACAAAT CCTACCGGCC GCTCTGCACT CTTTCTTTTC GACTGAATCA TGCCATCGGA 
GGGCTGAATC CCTGGAGTTA TCACCTTGTC AATGTCCTGT TGCACGCGGC CGTCACCGGC 
CTCTTCACAC GCTTCTCCAA GGCCCTGCTT GGGGACGGAT ACTGGACTTT CATGGCGGGC 
TTGATGTTTG CCTCGCACCC CATTCACACG GAGGCAGTGG CGGGCATCGT GGGCCGGGCA 
GACGTGGGCG CCAGTCTCTT TTTCCTCCTG TCCTTGCTCT GCTACACCAA GCACTGTTCT 
ACAAGAGGCT ACTCAGCCAG AACTTGGGGC TGGTTCCTGG GGACAGGACT GTGCGCTGGA 
TGCAGCATGC TGTGGAAGGA GCAAGGAGTG ACCGTCCTCG CAGTTTCAGC CGTTTATGAC 
GTCTTTGTCT TCCACAGGCT GAAGATGAGG CAGATTCTTC CTACCATTTA CAAAAGGAAG 
AACTTGTCTC TCTTTCTCAG CATTAGCTTG TTAACATTCT GGGGGACCAG CCTTTTGGGT 
GCCCGGTTAT ACTGGATGGG CAATAAACCA CCAAGCTTTT CCAACTCCGA CAACCCCGCT 
GCTGACTCGG ACAGCCTTTT GGCTCGCACG CTCACTTTCT TCTATTTACC TACCAAGAAC 
CTCTGGCTAT TGCTCTGCCC GGACACCCTG AGCTTTGATT GGTCCATGGA CGCCGTGCCT 
CTTCTCAAAA CAGTTTGTGA TTGGAGAAAT CTACACACTG TGGCCTTCTA TTCTGGACTT 
CTCCTGCTCG CCTACTGTGG TTTGAAGAGC CCAAGTTTAG AAGGAGAGTG CAACGGGAAA 
GCTGTGACAA ACGGCAAGCA GAACGCCAAT GGCCATAGTT GCTACTCAGA CGTGGAGTAC 
CGGAACTCAG AAATTAAGCC CAGCTTTGCA TCCAAAGTAG AGAATGGAAT TAAAAGTTGC 
GTATCACAGA GACCTCAGCT CCCTTCCACT GAGAACATTG TCATCCTGTC TTTATCTTTG 
TTGATAATAC CTTTTATTCC TGCCACGAAC CTGTTTTTCT ATGTCGGCTT TGTAATCGCA 
GAGCGAGTCT TATATATTCC CAGTATGGGC TTCTGCCTTC TGATCACAGT GGGCGCCAGA 
GCCCTTTATG TCAAAGTACA AAAGAGGTTT CTCAAGAGCC TGGTTTTTTA TGCTACAGCA 
ACGTTAATTG TCTTCTACGG ACTCAAGACT GCGATCAGGA ACGGAGACTG GCAGAATGAG 
GAGATGCTGT ATAGATCAGG GATCAAAGTG AACCCCGCCA AAGCATGGGG TAACCTTGGA 
AATGTTCTGA AGAGTCAGAG TAAAATCTCT GAAGCTGAAA TCGCCTATAG AAATGCTCTG 
TACTACCGCA GCAACATGGC TGACATGCTG TATAATTTAG GGTTGCTGCT CCAAGAGAAC 
AGCAGGTTTG CAGAAGCTCT CCATTATTAC AAACTGGCTG TCGGCAGCAG GCCTACCCTG 
GCTTCTGCCT ATTTAAACAC CGGCATCATT CTCATGAACC AAGGGAAGAC AGAAGAAGCC 
CGACGTACAT TCCTCAAGTG TTCTGAGATT CCAGATGAGA ATCTGAAGGA TCCTCATGCA 
CATAAGAGTT CTGTTACCAG CTGCCTGTAC AACCTCGGGA AACTCTATCA TGAGCAAGGA 
CGCTATGAGG AAGCCCTGAG TGTTTATAGG GAAGCAATTC AGAAGATGCC AAGGCAGTTT 
GCCCCTCAGA GCTTATACAA CATGATGGGC GAAGCCTACA TGAGACTGAG TAAGCTTCCT 
GAAGCTGAGC ATTGGTACAT GGAATCATTG AGATCCAAGA CGGACCACAT CCCTGCTCAT 
CTTACCTATG GGAAGCTGCT GGCGCTAACA GGTCGTAAGA GTGAGGCTGA AAAGTTCTTC 
TTGAAGGCTA TTGAGCTGGA TCCTACCAAA GGAAACTGTT ACATGCACTA TGGTCAGTTT 
CTCCTGGAGG AATCTCGCCT CACAGAAGCA GCCGAGATGG CAAAAAAAGC AGCTGAGCTG 
GACAGCACAG AGTTCGATGT CGTCTTCAAT GCTGCCACAT GCTCAGACAA GCTAGCCTCA 
ATGAAGCTGC GGAGAAGTAT TACGATCTTG CAGCAAGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017450228.1
CDS	641..2962
Translation	MIAELVSSALGLALYLNTLSADFCYDDSRAIKTNQDLLPETPWTHIFYNDFWGTLLTHSGSHKSYRPLCTLSFRLNHAIGGLNPWSYHLVNVLLHAAVTGLFTRFSKALLGDGYWTFMAGLMFASHPIHTEAVAGIVGRADVGASLFFLLSLLCYTKHCSTRGYSARTWGWFLGTGLCAGCSMLWKEQGVTVLAVSAVYDVFVFHRLKMRQILPTIYKRKNLSLFLSISLLTFWGTSLLGARLYWMGNKPPSFSNSDNPAADSDSLLARTLTFFYLPTKNLWLLLCPDTLSFDWSMDAVPLLKTVCDWRNLHTVAFYSGLLLLAYCGLKSPSLEGECNGKAVTNGKQNANGHSCYSDVEYRNSEIKPSFASKVENGIKSCVSQRPQLPSTENIVILSLSLLIIPFIPATNLFFYVGFVIAERVLYIPSMGFCLLITVGARALYVKVQKRFLKSLVFYATATLIVFYGLKTAIRNGDWQNEEMLYRSGIKVNPAKAWGNLGNVLKSQSKISEAEIAYRNALYYRSNMADMLYNLGLLLQENSRFAEALHYYKLAVGSRPTLASAYLNTGIILMNQGKTEEARRTFLKCSEIPDENLKDPHAHKSSVTSCLYNLGKLYHEQGRYEEALSVYREAIQKMPRQFAPQSLYNMMGEAYMRLSKLPEAEHWYMESLRSKTDHIPAHLTYGKLLALTGRKSEAEKFFLKAIELDPTKGNCYMHYGQFLLEESRLTEAAEMAKKAAELDSTEFDVVFNAATCSDKLASMKLRRSITILQQD

Target ORF information:

RefSeq Version	XM_017594739.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus transmembrane and tetratricopeptide repeat containing 2 (Tmtc2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017594739.1

ORF Insert Sequence:

ATGATTGCAG AGTTGGTGAG CAGCGCTCTG GGGCTCGCCT TGTATCTCAA CACCCTGAGT 
GCGGATTTCT GCTACGATGA CAGCCGTGCT ATCAAGACTA ATCAGGACCT GCTGCCAGAA 
ACCCCCTGGA CCCACATTTT CTATAATGAT TTCTGGGGGA CTCTTCTAAC CCACAGTGGT 
AGCCACAAAT CCTACCGGCC GCTCTGCACT CTTTCTTTTC GACTGAATCA TGCCATCGGA 
GGGCTGAATC CCTGGAGTTA TCACCTTGTC AATGTCCTGT TGCACGCGGC CGTCACCGGC 
CTCTTCACAC GCTTCTCCAA GGCCCTGCTT GGGGACGGAT ACTGGACTTT CATGGCGGGC 
TTGATGTTTG CCTCGCACCC CATTCACACG GAGGCAGTGG CGGGCATCGT GGGCCGGGCA 
GACGTGGGCG CCAGTCTCTT TTTCCTCCTG TCCTTGCTCT GCTACACCAA GCACTGTTCT 
ACAAGAGGCT ACTCAGCCAG AACTTGGGGC TGGTTCCTGG GGACAGGACT GTGCGCTGGA 
TGCAGCATGC TGTGGAAGGA GCAAGGAGTG ACCGTCCTCG CAGTTTCAGC CGTTTATGAC 
GTCTTTGTCT TCCACAGGCT GAAGATGAGG CAGATTCTTC CTACCATTTA CAAAAGGAAG 
AACTTGTCTC TCTTTCTCAG CATTAGCTTG TTAACATTCT GGGGGACCAG CCTTTTGGGT 
GCCCGGTTAT ACTGGATGGG CAATAAACCA CCAAGCTTTT CCAACTCCGA CAACCCCGCT 
GCTGACTCGG ACAGCCTTTT GGCTCGCACG CTCACTTTCT TCTATTTACC TACCAAGAAC 
CTCTGGCTAT TGCTCTGCCC GGACACCCTG AGCTTTGATT GGTCCATGGA CGCCGTGCCT 
CTTCTCAAAA CAGTTTGTGA TTGGAGAAAT CTACACACTG TGGCCTTCTA TTCTGGACTT 
CTCCTGCTCG CCTACTGTGG TTTGAAGAGC CCAAGTTTAG AAGGAGAGTG CAACGGGAAA 
GCTGTGACAA ACGGCAAGCA GAACGCCAAT GGCCATAGTT GCTACTCAGA CGTGGAGTAC 
CGGAACTCAG AAATTAAGCC CAGCTTTGCA TCCAAAGTAG AGAATGGAAT TAAAAGTTGC 
GTATCACAGA GACCTCAGCT CCCTTCCACT GAGAACATTG TCATCCTGTC TTTATCTTTG 
TTGATAATAC CTTTTATTCC TGCCACGAAC CTGTTTTTCT ATGTCGGCTT TGTAATCGCA 
GAGCGAGTCT TATATATTCC CAGTATGGGC TTCTGCCTTC TGATCACAGT GGGCGCCAGA 
GCCCTTTATG TCAAAGTACA AAAGAGGTTT CTCAAGAGCC TGGTTTTTTA TGCTACAGCA 
ACGTTAATTG TCTTCTACGG ACTCAAGACT GCGATCAGGA ACGGAGACTG GCAGAATGAG 
GAGATGCTGT ATAGATCAGG GATCAAAGTG AACCCCGCCA AAGCATGGGG TAACCTTGGA 
AATGTTCTGA AGAGTCAGAG TAAAATCTCT GAAGCTGAAA TCGCCTATAG AAATGCTCTG 
TACTACCGCA GCAACATGGC TGACATGCTG TATAATTTAG GGTTGCTGCT CCAAGAGAAC 
AGCAGGTTTG CAGAAGCTCT CCATTATTAC AAACTGGCTG TCGGCAGCAG GCCTACCCTG 
GCTTCTGCCT ATTTAAACAC CGGCATCATT CTCATGAACC AAGGGAAGAC AGAAGAAGCC 
CGACGTACAT TCCTCAAGTG TTCTGAGATT CCAGATGAGA ATCTGAAGGA TCCTCATGCA 
CATAAGAGTT CTGTTACCAG CTGCCTGTAC AACCTCGGGA AACTCTATCA TGAGCAAGGA 
CGCTATGAGG AAGCCCTGAG TGTTTATAGG GAAGCAATTC AGAAGATGCC AAGGCAGTTT 
GCCCCTCAGA GCTTATACAA CATGATGGGC GAAGCCTACA TGAGACTGAG TAAGCTTCCT 
GAAGCTGAGC ATTGGTACAT GGAATCATTG AGATCCAAGA CGGACCACAT CCCTGCTCAT 
CTTACCTATG GGAAGCTGCT GGCGCTAACA GGTCGTAAGA GTGAGGCTGA AAAGTTCTTC 
TTGAAGGCTA TTGAGCTGGA TCCTACCAAA GGAAACTGTT ACATGCACTA TGGTCAGTTT 
CTCCTGGAGG AATCTCGCCT CACAGAAGCA GCCGAGATGG CAAAAAAAGC AGCTGAGCTG 
GACAGCACAG AGTTCGATGT CGTCTTCAAT GCTGCCACAT GCTCAGACAA GCTAGCCTCA 
ATGAAGCTGC GGAGAAGTAT TACGATCTTG CAGCAAGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Discovery of an O-mannosylation pathway selectively serving cadherins and protocadherins.
Proceedings of the National Academy of Sciences of the United States of America114(42)11163-11168(2017 Oct)
Larsen ISB,Narimatsu Y,Joshi HJ,Siukstaite L,Harrison OJ,Brasch J,Goodman KM,Hansen L,Shapiro L,Honig B,Vakhrushev SY,Clausen H,Halim A

Association of TMTC2 With Human Nonsyndromic Sensorineural Hearing Loss.
JAMA otolaryngology-- head & neck surgery142(9)866-72(2016 Sep)
Runge CL,Indap A,Zhou Y,Kent JW Jr,King E,Erbe CB,Cole R,Littrell J,Merath K,James R,R?schendorf F,Kerschner JE,Marth G,H?bner N,G?ring HH,Friedland DR,Kwok WM,Olivier M

TMTC1 and TMTC2 are novel endoplasmic reticulum tetratricopeptide repeat-containing adapter proteins involved in calcium homeostasis.
The Journal of biological chemistry289(23)16085-99(2014 Jun)
Sunryd JC,Cheon B,Graham JB,Giorda KM,Fissore RA,Hebert DN

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Tmtc2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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