Mycn cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mycn
Entrez Gene ID	298894
Full Name	MYCN proto-oncogene, bHLH transcription factor
Synonyms	N-myc,Nmuc1,Nmyc
General protein information	Preferred Names MYCN proto-oncogene, bHLH transcription factor Names N-myc proto-oncogene protein Avian myelocytomatosis viral (v-myc) related oncogene, neuroblastoma derived (Nmyc) v-myc avian myelocytomatosis viral oncogene neuroblastoma derived homolog v-myc myelocytomatosis viral related oncogene, neuroblastoma derived
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 6 Map Location: 6q15

mRNA and Protein(s)

mRNA	Protein	Name
XM_008764586.2	XP_008762808.1	N-myc proto-oncogene protein isoform X1
NM_001013096.1	NP_001013114.1	N-myc proto-oncogene protein

Pathways from BioSystems

Homologies

Homo sapiens (human)	MYCN	NP_005369.2
	MYCN	XP_001091369.2
Mus musculus (house mouse)	Mycn	NP_032735.3
Gallus gallus (chicken)	MYCN	NP_001026262.1
Pan troglodytes (chimpanzee)	MYCN	XP_001162405.1
Canis lupus familiaris (dog)	MYCN	XP_005630203.1
Rattus norvegicus (Norway rat)	Mycn	NP_001013114.1
Danio rerio (zebrafish)	mycn	NP_997779.1
Bos taurus (cattle)	MYCN	XP_002691569.1
Xenopus tropicalis (tropical clawed frog)	mycn	NP_001006874.1

Gene Ontology
Bibliography

Related articles in PubMed

Structural basis of N-Myc binding by Aurora-A and its destabilization by kinase inhibitors.
Richards MW, Burgess SG, Poon E, Carstensen A, Eilers M, Chesler L, Bayliss R
Proceedings of the National Academy of Sciences of the United States of America113(48)13726-13731(2016 Nov)

NCYM, a Cis-antisense gene of MYCN, encodes a de novo evolved protein that inhibits GSK3? resulting in the stabilization of MYCN in human neuroblastomas.
Suenaga Y, Islam SM, Alagu J, Kaneko Y, Kato M, Tanaka Y, Kawana H, Hossain S, Matsumoto D, Yamamoto M, Shoji W, Itami M, Shibata T, Nakamura Y, Ohira M, Haraguchi S, Takatori A, Nakagawara A
PLoS genetics10(1)e1003996(2014 Jan)

Focal DNA copy number changes in neuroblastoma target MYCN regulated genes.
Kumps C, Fieuw A, Mestdagh P, Menten B, Lefever S, Pattyn F, De Brouwer S, Sante T, Schulte JH, Schramm A, Van Roy N, Van Maerken T, Noguera R, Combaret V, Devalck C, Westermann F, Laureys G, Eggert A, Vandesompele J, De Preter K, Speleman F
PloS one8(1)e52321(2013)

N-Myc and L-Myc are essential for hair cell formation but not maintenance.
Kopecky BJ, Decook R, Fritzsch B
Brain research14841-14(2012 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that nitric oxide negatively regulates proliferation and promotes neuronal differentiation through N-Myc downregulation.
Title: Nitric oxide negatively regulates proliferation and promotes neuronal differentiation through N-Myc downregulation.

The following Mycn gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mycn cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa03351	NM_001013096.1 Latest version!	Rattus norvegicus MYCN proto-oncogene, bHLH transcription factor (Mycn), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
ORa45578	XM_008764586.2 Latest version!	Rattus norvegicus v-myc avian myelocytomatosis viral oncogene neuroblastoma derived homolog (Mycn), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mycn ( NM_001013096.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001013114.1 Rattus norvegicus MYCN proto-oncogene, bHLH transcription factor (Mycn), mRNA.

Price & Availability↑

CloneID	ORa03351
Clone ID Related Accession (Same CDS sequence)	NM_001013096.1
Accession Version	NM_001013096.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1389bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	N-myc proto-oncogene protein
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X63281.1. On Apr 20, 2005 this sequence version replaced XM_234025.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGCCCAGCT GCACCGCGTC CACCATGCCG GGGATGATCT GCAAGAACCC AGACCTCGAG 
TTTGACTCCC TGCAGCCCTG CTTCTACCCG GACGAAGATG ACTTCTACTT CGGCGGTCCC 
GACTCGACCC CACCAGGGGA GGACATCTGG AAGAAGTTTG AGCTGCTGCC CACGCCCCCG 
TTGTCGCCCA GCCGCGCCTT CCCAGAGCAC AGCCCGGAGC CCTCAAATTG GGCCACGGAG 
ATGCTGCTGC CGGAGGCCGA CCTGTGGGGC AACCCGGCCG AGGAGGATGC GTTTGGTCTG 
GGGGGACTGG GTGGCCTCAC TCCTAATCCG GTCATCCTTC AGGACTGCAT GTGGAGCGGC 
TTCTCTGCCC GCGAGAAGCT GGAGCGCGCA GTGAACGAGA AGCTGCAGCA CGGCCACGGG 
CCCCCGGGAG CCAGCTCATC CTGTCCGGCT CCGGGAGTGG GTGCCAGTAG CTCCGGGGGC 
CGTGCCCTTG GTGGGTCGGC GAGTGCTGGT CGCACCGGGG CCACCCTGCC TACCGACCTT 
TCCCACCCGG CTGCGGAGTG TGTGGATCCC GCCGTGGTCT TCCCCTTCCC GGTGAACAAG 
CGAGAGTCGG CTTCGGTGCC CGCTGCCCCG ACCAGTGCCC CGGCGACCAG CGCTGTGGTC 
ACTAGTGTGT CTGTCCCGGC TGTAGCCCCG GTGGCTGCTC CTGCTCGTGG CAGCGGTCGT 
CCTGCCAACA GTGGCGAGCA CAAGGCCCTC AGCACCTCTG GAGAGGATAC CTTGAGCGAC 
TCAGATGATG AAGACGATGA GGAGGAAGAT GAAGAGGAGG AGATCGATGT GGTCACGGTA 
GAGAAGAGAC GCTCCTCCTC TAACAACAAG GCAGTCACCA CTTTCACCAT CACTGTACGC 
CCCAAGACCT CGGCTCTGGG CCTGGGGCGA GCACAACCTG GCGAGCTGAT CCTCAAGCGC 
TGTGTCCCCA TCCATCAGCA ACACAACTAT GCCGCACCCT CACCCTATGT GGAGAGCGAG 
GATGCGCCAC CACAGAAGAA GATCAAGAGC GAGGCTTCTC CACGCCCCCT CAAAAGTGTC 
GTTCCACCAA AGGCTAAGAG CTTGAGCCCC CGAAACTCAG ACTCGGAGGA CAGCGAGCGC 
CGCCGAAACC ACAACATCCT GGAGCGCCAG CGCCGCAACG ACCTGCGCTC CAGCTTCCTG 
ACGCTCAGGG ACCATGTGCC CGAGCTGGTG AAGAACGAGA AGGCCGCCAA GGTGGTCATC 
TTAAAAAAGG CCACCGAGTA CGTCCACGCC CTACAGGCCA ACGAGCACCA GCTCCTGCTG 
GAAAAGGAGA AACTGCAGGC GAGGCAGCAG CAGTTGCTAA AGAAGATCGA ACACGCTCGG 
ACTTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001013114.1
CDS	291..1679
Translation	MPSCTASTMPGMICKNPDLEFDSLQPCFYPDEDDFYFGGPDSTPPGEDIWKKFELLPTPPLSPSRAFPEHSPEPSNWATEMLLPEADLWGNPAEEDAFGLGGLGGLTPNPVILQDCMWSGFSAREKLERAVNEKLQHGHGPPGASSSCPAPGVGASSSGGRALGGSASAGRTGATLPTDLSHPAAECVDPAVVFPFPVNKRESASVPAAPTSAPATSAVVTSVSVPAVAPVAAPARGSGRPANSGEHKALSTSGEDTLSDSDDEDDEEEDEEEEIDVVTVEKRRSSSNNKAVTTFTITVRPKTSALGLGRAQPGELILKRCVPIHQQHNYAAPSPYVESEDAPPQKKIKSEASPRPLKSVVPPKAKSLSPRNSDSEDSERRRNHNILERQRRNDLRSSFLTLRDHVPELVKNEKAAKVVILKKATEYVHALQANEHQLLLEKEKLQARQQQLLKKIEHARTC

Target ORF information:

RefSeq Version	NM_001013096.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus MYCN proto-oncogene, bHLH transcription factor (Mycn), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001013096.1

ORF Insert Sequence:

ATGCCCAGCT GCACCGCGTC CACCATGCCG GGGATGATCT GCAAGAACCC AGACCTCGAG 
TTTGACTCCC TGCAGCCCTG CTTCTACCCG GACGAAGATG ACTTCTACTT CGGCGGTCCC 
GACTCGACCC CACCAGGGGA GGACATCTGG AAGAAGTTTG AGCTGCTGCC CACGCCCCCG 
TTGTCGCCCA GCCGCGCCTT CCCAGAGCAC AGCCCGGAGC CCTCAAATTG GGCCACGGAG 
ATGCTGCTGC CGGAGGCCGA CCTGTGGGGC AACCCGGCCG AGGAGGATGC GTTTGGTCTG 
GGGGGACTGG GTGGCCTCAC TCCTAATCCG GTCATCCTTC AGGACTGCAT GTGGAGCGGC 
TTCTCTGCCC GCGAGAAGCT GGAGCGCGCA GTGAACGAGA AGCTGCAGCA CGGCCACGGG 
CCCCCGGGAG CCAGCTCATC CTGTCCGGCT CCGGGAGTGG GTGCCAGTAG CTCCGGGGGC 
CGTGCCCTTG GTGGGTCGGC GAGTGCTGGT CGCACCGGGG CCACCCTGCC TACCGACCTT 
TCCCACCCGG CTGCGGAGTG TGTGGATCCC GCCGTGGTCT TCCCCTTCCC GGTGAACAAG 
CGAGAGTCGG CTTCGGTGCC CGCTGCCCCG ACCAGTGCCC CGGCGACCAG CGCTGTGGTC 
ACTAGTGTGT CTGTCCCGGC TGTAGCCCCG GTGGCTGCTC CTGCTCGTGG CAGCGGTCGT 
CCTGCCAACA GTGGCGAGCA CAAGGCCCTC AGCACCTCTG GAGAGGATAC CTTGAGCGAC 
TCAGATGATG AAGACGATGA GGAGGAAGAT GAAGAGGAGG AGATCGATGT GGTCACGGTA 
GAGAAGAGAC GCTCCTCCTC TAACAACAAG GCAGTCACCA CTTTCACCAT CACTGTACGC 
CCCAAGACCT CGGCTCTGGG CCTGGGGCGA GCACAACCTG GCGAGCTGAT CCTCAAGCGC 
TGTGTCCCCA TCCATCAGCA ACACAACTAT GCCGCACCCT CACCCTATGT GGAGAGCGAG 
GATGCGCCAC CACAGAAGAA GATCAAGAGC GAGGCTTCTC CACGCCCCCT CAAAAGTGTC 
GTTCCACCAA AGGCTAAGAG CTTGAGCCCC CGAAACTCAG ACTCGGAGGA CAGCGAGCGC 
CGCCGAAACC ACAACATCCT GGAGCGCCAG CGCCGCAACG ACCTGCGCTC CAGCTTCCTG 
ACGCTCAGGG ACCATGTGCC CGAGCTGGTG AAGAACGAGA AGGCCGCCAA GGTGGTCATC 
TTAAAAAAGG CCACCGAGTA CGTCCACGCC CTACAGGCCA ACGAGCACCA GCTCCTGCTG 
GAAAAGGAGA AACTGCAGGC GAGGCAGCAG CAGTTGCTAA AGAAGATCGA ACACGCTCGG 
ACTTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mycn ( XM_008764586.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008762808.1 Rattus norvegicus v-myc avian myelocytomatosis viral oncogene neuroblastoma derived homolog (Mycn), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa45578
Clone ID Related Accession (Same CDS sequence)	XM_008764586.2
Accession Version	XM_008764586.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 774bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	N-myc proto-oncogene protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005105.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008764586.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGGGGG CTCCTGGGAA CTGGGTTGGA GCCGAACGAG CGCTAGCCAG GCGTAAGCGC 
GCACACACTG CAGCGTCCCG AGGACGACCC CCCTCCCACC GCCGCCCCCT CAGCCCACCC 
GGAGACCCCT GCACCGAGTC GCCTCCGGAT CCCCGGCAGT CTGCGGGAGA TGATGAAGAC 
GATGAGGAGG AAGATGAAGA GGAGGAGATC GATGTGGTCA CGGTAGAGAA GAGACGCTCC 
TCCTCTAACA ACAAGGCAGT CACCACTTTC ACCATCACTG TACGCCCCAA GACCTCGGCT 
CTGGGCCTGG GGCGAGCACA ACCTGGCGAG CTGATCCTCA AGCGCTGTGT CCCCATCCAT 
CAGCAACACA ACTATGCCGC ACCCTCACCC TATGTGGAGA GCGAGGATGC GCCACCACAG 
AAGAAGATCA AGAGCGAGGC TTCTCCACGC CCCCTCAAAA GTGTCGTTCC ACCAAAGGCT 
AAGAGCTTGA GCCCCCGAAA CTCAGACTCG GAGGACAGCG AGCGCCGCCG AAACCACAAC 
ATCCTGGAGC GCCAGCGCCG CAACGACCTG CGCTCCAGCT TCCTGACGCT CAGGGACCAT 
GTGCCCGAGC TGGTGAAGAA CGAGAAGGCC GCCAAGGTGG TCATCTTAAA AAAGGCCACC 
GAGTACGTCC ACGCCCTACA GGCCAACGAG CACCAGCTCC TGCTGGAAAA GGAGAAACTG 
CAGGCGAGGC AGCAGCAGTT GCTAAAGAAG ATCGAACACG CTCGGACTTG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008762808.1
CDS	77..850
Translation	MRGAPGNWVGAERALARRKRAHTAASRGRPPSHRRPLSPPGDPCTESPPDPRQSAGDDEDDEEEDEEEEIDVVTVEKRRSSSNNKAVTTFTITVRPKTSALGLGRAQPGELILKRCVPIHQQHNYAAPSPYVESEDAPPQKKIKSEASPRPLKSVVPPKAKSLSPRNSDSEDSERRRNHNILERQRRNDLRSSFLTLRDHVPELVKNEKAAKVVILKKATEYVHALQANEHQLLLEKEKLQARQQQLLKKIEHARTC

Target ORF information:

RefSeq Version	XM_008764586.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus v-myc avian myelocytomatosis viral oncogene neuroblastoma derived homolog (Mycn), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008764586.2

ORF Insert Sequence:

ATGCGGGGGG CTCCTGGGAA CTGGGTTGGA GCCGAACGAG CGCTAGCCAG GCGTAAGCGC 
GCACACACTG CAGCGTCCCG AGGACGACCC CCCTCCCACC GCCGCCCCCT CAGCCCACCC 
GGAGACCCCT GCACCGAGTC GCCTCCGGAT CCCCGGCAGT CTGCGGGAGA TGATGAAGAC 
GATGAGGAGG AAGATGAAGA GGAGGAGATC GATGTGGTCA CGGTAGAGAA GAGACGCTCC 
TCCTCTAACA ACAAGGCAGT CACCACTTTC ACCATCACTG TACGCCCCAA GACCTCGGCT 
CTGGGCCTGG GGCGAGCACA ACCTGGCGAG CTGATCCTCA AGCGCTGTGT CCCCATCCAT 
CAGCAACACA ACTATGCCGC ACCCTCACCC TATGTGGAGA GCGAGGATGC GCCACCACAG 
AAGAAGATCA AGAGCGAGGC TTCTCCACGC CCCCTCAAAA GTGTCGTTCC ACCAAAGGCT 
AAGAGCTTGA GCCCCCGAAA CTCAGACTCG GAGGACAGCG AGCGCCGCCG AAACCACAAC 
ATCCTGGAGC GCCAGCGCCG CAACGACCTG CGCTCCAGCT TCCTGACGCT CAGGGACCAT 
GTGCCCGAGC TGGTGAAGAA CGAGAAGGCC GCCAAGGTGG TCATCTTAAA AAAGGCCACC 
GAGTACGTCC ACGCCCTACA GGCCAACGAG CACCAGCTCC TGCTGGAAAA GGAGAAACTG 
CAGGCGAGGC AGCAGCAGTT GCTAAAGAAG ATCGAACACG CTCGGACTTG CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Structural basis of N-Myc binding by Aurora-A and its destabilization by kinase inhibitors.
Proceedings of the National Academy of Sciences of the United States of America113(48)13726-13731(2016 Nov)
Richards MW,Burgess SG,Poon E,Carstensen A,Eilers M,Chesler L,Bayliss R

NCYM, a Cis-antisense gene of MYCN, encodes a de novo evolved protein that inhibits GSK3? resulting in the stabilization of MYCN in human neuroblastomas.
PLoS genetics10(1)e1003996(2014 Jan)
Suenaga Y,Islam SM,Alagu J,Kaneko Y,Kato M,Tanaka Y,Kawana H,Hossain S,Matsumoto D,Yamamoto M,Shoji W,Itami M,Shibata T,Nakamura Y,Ohira M,Haraguchi S,Takatori A,Nakagawara A

Focal DNA copy number changes in neuroblastoma target MYCN regulated genes.
PloS one8(1)e52321(2013)
Kumps C,Fieuw A,Mestdagh P,Menten B,Lefever S,Pattyn F,De Brouwer S,Sante T,Schulte JH,Schramm A,Van Roy N,Van Maerken T,Noguera R,Combaret V,Devalck C,Westermann F,Laureys G,Eggert A,Vandesompele J,De Preter K,Speleman F

N-Myc and L-Myc are essential for hair cell formation but not maintenance.
Brain research14841-14(2012 Nov)
Kopecky BJ,Decook R,Fritzsch B