Hif1a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hif1a
Entrez Gene ID	29560
Full Name	hypoxia inducible factor 1 alpha subunit
Synonyms	MOP1
General protein information	Preferred Names hypoxia inducible factor 1 alpha subunit Names hypoxia-inducible factor 1-alpha HIF-1-alpha HIF1-alpha hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 6 Map Location: 6q24
Summary	regulates transcription in response to low oxygen; may play a role in vascular biology [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006240196.3	XP_006240258.1	hypoxia-inducible factor 1-alpha isoform X1
XM_006240197.3	XP_006240259.1	hypoxia-inducible factor 1-alpha isoform X2
NM_024359.1	NP_077335.1	hypoxia-inducible factor 1-alpha
XM_006240198.3	XP_006240260.1	hypoxia-inducible factor 1-alpha isoform X3
XM_006240199.3	XP_006240261.1	hypoxia-inducible factor 1-alpha isoform X4

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	HIF1A	XP_003639249.1
Homo sapiens (human)	HIF1A	NP_001521.1
Rattus norvegicus (Norway rat)	Hif1a	NP_077335.1
	HIF1A	XP_001099149.1
Bos taurus (cattle)	HIF1A	NP_776764.2
Mus musculus (house mouse)	Hif1a	NP_034561.2
Danio rerio (zebrafish)	hif1ab	NP_956527.1
Pan troglodytes (chimpanzee)	HIF1A	XP_001168972.1
Gallus gallus (chicken)	HIF1A	NP_989628.1

Gene Ontology

The following Hif1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hif1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa13392	NM_024359.1 Latest version!	Rattus norvegicus hypoxia inducible factor 1 subunit alpha (Hif1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$482.30 $689.00
ORa29394	XM_006240196.3 Latest version!	Rattus norvegicus hypoxia inducible factor 1 alpha subunit (Hif1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa29395	XM_006240197.3 Latest version!	Rattus norvegicus hypoxia inducible factor 1 alpha subunit (Hif1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa29396	XM_006240198.3 Latest version!	Rattus norvegicus hypoxia inducible factor 1 alpha subunit (Hif1a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa29397	XM_006240199.3 Latest version!	Rattus norvegicus hypoxia inducible factor 1 alpha subunit (Hif1a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hif1a ( NM_024359.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_077335.1 Rattus norvegicus hypoxia inducible factor 1 subunit alpha (Hif1a), mRNA.

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CloneID	ORa13392
Clone ID Related Accession (Same CDS sequence)	NM_024359.1
Accession Version	NM_024359.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2472bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	hypoxia-inducible factor 1-alpha
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF057308.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF057308.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGAGGGCG CCGGCGGCGA GAACGAGAAG AAAAATAGGA TGAGTTCCGA ACGTCGAAAA 
GAAAAGTCTA GGGATGCAGC ACGATCTCGG CGAAGCAAAG AGTCTGAAGT TTTTTATGAG 
CTTGCTCATC AGTTGCCACT TCCCCACAAC GTGAGCTCCC ATCTTGATAA AGCTTCTGTT 
ATGAGGCTCA CCATCAGTTA CTTACGTGTG AGGAAACTTC TAGGTGCTGG TGATCTTGAC 
ATTGAAGATG AAATGAAAGC ACAGATGAAC TGCTTTTATC TGAAAGCCCT GGATGGCTTT 
GTTATGGTGC TAACAGATGA TGGTGACATG ATTTACATTT CTGATAACGT GAACAAATAC 
ATGGGGTTGA CTCAGTTTGA ACTAACTGGA CACAGTGTGT TTGATTTTAC CCATCCATGT 
GACCATGAGG AAATGAGAGA AATGCTTACA CACAGAAATG GCCCAGTGAG AAAGGGGAAA 
GAACAAAACA CGCAGCGAAG CTTTTTTCTC AGAATGAAAT GTACCCTAAC AAGCCGGGGG 
AGGACGATGA ACATCAAGTC AGCAACGTGG AAGGTGCTGC ACTGCACAGG CCACATTCAT 
GTGTATGATA CCAGCAGTAA CCAGCCGCAG TGTGGCTACA AGAAACCGCC TATGACGTGC 
TTGGTGCTGA TTTGTGAACC CATTCCTCAT CCATCAAACA TTGAAATTCC TTTAGACAGC 
AAGACATTTC TCAGTCGACA CAGCCTCGAT ATGAAATTTT CTTACTGTGA TGAAAGGATT 
ACTGAGTTGA TGGGTTATGA GCCAGAAGAA CTTTTGGGCC GTTCAATTTA TGAATATTAT 
CATGCTTTGG ACTCTGATCA TCTGACCAAA ACTCATCATG ACATGTTTAC TAAAGGACAA 
GTCACCACAG GACAGTACAG GATGCTTGCA AAAAGAGGTG GATATGTCTG GGTTGAGACT 
CAAGCAACTG TTATATATAA TACGAAGAAC TCTCAGCCAC AGTGCATTGT GTGTGTGAAT 
TATGTTGTAA GTGGTATTAT TCAGCACGAC TTGATTTTCT CCCTTCAACA AACAGAATCT 
GTCCTCAAAC CAGTTGAATC TTCAGATATG AAAATGACCC AGCTGTTCAC TAAAGTGGAA 
TCTGAGGACA CGAGCTGCCT CTTCGACAAG CTTAAGAAAG AGCCCGATGC CCTGACTCTG 
CTAGCTCCAG CGGCTGGGGA CACGATCATA TCACTGGACT TCGGCAGCGA TGACACGGAA 
ACTGAAGACC AACAACTTGA AGATGTCCCG TTGTACAATG ATGTAATGTT CCCCTCTTCT 
AATGAGAAAT TAAATATAAA TCTGGCAATG TCTCCATTAC CTGCCTCTGA AACTCCAAAG 
CCACTTCGAA GTAGTGCTGA TCCTGCACTG AATCAAGAGG TTGCATTGAA GTTAGAGTCA 
AGCCCAGAGT CACTGGGACT TTCTTTTACC ATGCCCCAGA TTCAAGATCA GCCAGCAAGT 
CCTTCTGATG GAAGCACTAG ACAAAGCTCA CCTGAGCCTA ACAGTCCCAG TGAGTACTGC 
TTTGATGTGG ACAGCGATAT GGTCAATGTA TTCAAGTTGG AACTGGTGGA AAAACTGTTT 
GCTGAAGACA CAGAAGCGAA GAATCCATTT TCAGCTCAGG ACACTGATTT AGACTTGGAA 
ATGCTGGCTC CCTATATCCC AATGGATGAT GATTTCCAGT TACGTTCCTT TGATCAGTTG 
TCACCATTAG AGAGCAATTC TCCAAGCCCT CCGAGTGTGA GCACAGTTAC AGGATTCCAG 
CAGACCCAGT TACAGAAACC TACCATCACT GTCACTGCCA CCGCAACTGC CACCACTGAT 
GAATCAAAAG CAGTGACGAA GGACAATATA GAAGACATTA AAATACTGAT TGCATCTCCA 
CCTTCTACCC AAGTACCTCA AGAAATGACC ACTGCTAAGG CATCAGCATA CAGTGGTACT 
CACAGTCGGA CAGCCTCACC AGACAGAGCA GGAAAGAGAG TCATAGAAAA AACAGACAAA 
GCTCATCCAA GGAGCCTTAA CCTATCTGTC ACTTTGAATC AAAGAAATAC TGTTCCTGAA 
GAAGAATTAA ACCCAAAGAC AATAGCTTTG CAGAATGCTC AGAGGAAGCG AAAAATGGAA 
CATGATGGCT CCCTTTTTCA AGCAGCAGGA ATTGGAACGT TACTGCAGCA ACCAGGTGAC 
CGTGCCCCTA CTATGTCGCT TTCTTGGAAA CGAGTGAAAG GATACATATC TAGTGAACAG 
GATGGAATGG AGCAGAAGAC AATTTTTTTA ATACCCTCTG ATTTAGCATG TAGACTGCTG 
GGGCAGTCAA TGGATGAGAG TGGATTACCA CAGCTGACCA GTTACGATTG TGAAGTTAAT 
GCTCCCATAC AAGGCAGCAG AAACCTACTG CAGGGTGAAG AATTACTCAG AGCTTTGGAT 
CAAGTTAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_077335.1
CDS	24..2495
Translation	MEGAGGENEKKNRMSSERRKEKSRDAARSRRSKESEVFYELAHQLPLPHNVSSHLDKASVMRLTISYLRVRKLLGAGDLDIEDEMKAQMNCFYLKALDGFVMVLTDDGDMIYISDNVNKYMGLTQFELTGHSVFDFTHPCDHEEMREMLTHRNGPVRKGKEQNTQRSFFLRMKCTLTSRGRTMNIKSATWKVLHCTGHIHVYDTSSNQPQCGYKKPPMTCLVLICEPIPHPSNIEIPLDSKTFLSRHSLDMKFSYCDERITELMGYEPEELLGRSIYEYYHALDSDHLTKTHHDMFTKGQVTTGQYRMLAKRGGYVWVETQATVIYNTKNSQPQCIVCVNYVVSGIIQHDLIFSLQQTESVLKPVESSDMKMTQLFTKVESEDTSCLFDKLKKEPDALTLLAPAAGDTIISLDFGSDDTETEDQQLEDVPLYNDVMFPSSNEKLNINLAMSPLPASETPKPLRSSADPALNQEVALKLESSPESLGLSFTMPQIQDQPASPSDGSTRQSSPEPNSPSEYCFDVDSDMVNVFKLELVEKLFAEDTEAKNPFSAQDTDLDLEMLAPYIPMDDDFQLRSFDQLSPLESNSPSPPSVSTVTGFQQTQLQKPTITVTATATATTDESKAVTKDNIEDIKILIASPPSTQVPQEMTTAKASAYSGTHSRTASPDRAGKRVIEKTDKAHPRSLNLSVTLNQRNTVPEEELNPKTIALQNAQRKRKMEHDGSLFQAAGIGTLLQQPGDRAPTMSLSWKRVKGYISSEQDGMEQKTIFLIPSDLACRLLGQSMDESGLPQLTSYDCEVNAPIQGSRNLLQGEELLRALDQVN

Target ORF information:

RefSeq Version	NM_024359.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus hypoxia inducible factor 1 subunit alpha (Hif1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_024359.1

ORF Insert Sequence:

ATGGAGGGCG CCGGCGGCGA GAACGAGAAG AAAAATAGGA TGAGTTCCGA ACGTCGAAAA 
GAAAAGTCTA GGGATGCAGC ACGATCTCGG CGAAGCAAAG AGTCTGAAGT TTTTTATGAG 
CTTGCTCATC AGTTGCCACT TCCCCACAAC GTGAGCTCCC ATCTTGATAA AGCTTCTGTT 
ATGAGGCTCA CCATCAGTTA CTTACGTGTG AGGAAACTTC TAGGTGCTGG TGATCTTGAC 
ATTGAAGATG AAATGAAAGC ACAGATGAAC TGCTTTTATC TGAAAGCCCT GGATGGCTTT 
GTTATGGTGC TAACAGATGA TGGTGACATG ATTTACATTT CTGATAACGT GAACAAATAC 
ATGGGGTTGA CTCAGTTTGA ACTAACTGGA CACAGTGTGT TTGATTTTAC CCATCCATGT 
GACCATGAGG AAATGAGAGA AATGCTTACA CACAGAAATG GCCCAGTGAG AAAGGGGAAA 
GAACAAAACA CGCAGCGAAG CTTTTTTCTC AGAATGAAAT GTACCCTAAC AAGCCGGGGG 
AGGACGATGA ACATCAAGTC AGCAACGTGG AAGGTGCTGC ACTGCACAGG CCACATTCAT 
GTGTATGATA CCAGCAGTAA CCAGCCGCAG TGTGGCTACA AGAAACCGCC TATGACGTGC 
TTGGTGCTGA TTTGTGAACC CATTCCTCAT CCATCAAACA TTGAAATTCC TTTAGACAGC 
AAGACATTTC TCAGTCGACA CAGCCTCGAT ATGAAATTTT CTTACTGTGA TGAAAGGATT 
ACTGAGTTGA TGGGTTATGA GCCAGAAGAA CTTTTGGGCC GTTCAATTTA TGAATATTAT 
CATGCTTTGG ACTCTGATCA TCTGACCAAA ACTCATCATG ACATGTTTAC TAAAGGACAA 
GTCACCACAG GACAGTACAG GATGCTTGCA AAAAGAGGTG GATATGTCTG GGTTGAGACT 
CAAGCAACTG TTATATATAA TACGAAGAAC TCTCAGCCAC AGTGCATTGT GTGTGTGAAT 
TATGTTGTAA GTGGTATTAT TCAGCACGAC TTGATTTTCT CCCTTCAACA AACAGAATCT 
GTCCTCAAAC CAGTTGAATC TTCAGATATG AAAATGACCC AGCTGTTCAC TAAAGTGGAA 
TCTGAGGACA CGAGCTGCCT CTTCGACAAG CTTAAGAAAG AGCCCGATGC CCTGACTCTG 
CTAGCTCCAG CGGCTGGGGA CACGATCATA TCACTGGACT TCGGCAGCGA TGACACGGAA 
ACTGAAGACC AACAACTTGA AGATGTCCCG TTGTACAATG ATGTAATGTT CCCCTCTTCT 
AATGAGAAAT TAAATATAAA TCTGGCAATG TCTCCATTAC CTGCCTCTGA AACTCCAAAG 
CCACTTCGAA GTAGTGCTGA TCCTGCACTG AATCAAGAGG TTGCATTGAA GTTAGAGTCA 
AGCCCAGAGT CACTGGGACT TTCTTTTACC ATGCCCCAGA TTCAAGATCA GCCAGCAAGT 
CCTTCTGATG GAAGCACTAG ACAAAGCTCA CCTGAGCCTA ACAGTCCCAG TGAGTACTGC 
TTTGATGTGG ACAGCGATAT GGTCAATGTA TTCAAGTTGG AACTGGTGGA AAAACTGTTT 
GCTGAAGACA CAGAAGCGAA GAATCCATTT TCAGCTCAGG ACACTGATTT AGACTTGGAA 
ATGCTGGCTC CCTATATCCC AATGGATGAT GATTTCCAGT TACGTTCCTT TGATCAGTTG 
TCACCATTAG AGAGCAATTC TCCAAGCCCT CCGAGTGTGA GCACAGTTAC AGGATTCCAG 
CAGACCCAGT TACAGAAACC TACCATCACT GTCACTGCCA CCGCAACTGC CACCACTGAT 
GAATCAAAAG CAGTGACGAA GGACAATATA GAAGACATTA AAATACTGAT TGCATCTCCA 
CCTTCTACCC AAGTACCTCA AGAAATGACC ACTGCTAAGG CATCAGCATA CAGTGGTACT 
CACAGTCGGA CAGCCTCACC AGACAGAGCA GGAAAGAGAG TCATAGAAAA AACAGACAAA 
GCTCATCCAA GGAGCCTTAA CCTATCTGTC ACTTTGAATC AAAGAAATAC TGTTCCTGAA 
GAAGAATTAA ACCCAAAGAC AATAGCTTTG CAGAATGCTC AGAGGAAGCG AAAAATGGAA 
CATGATGGCT CCCTTTTTCA AGCAGCAGGA ATTGGAACGT TACTGCAGCA ACCAGGTGAC 
CGTGCCCCTA CTATGTCGCT TTCTTGGAAA CGAGTGAAAG GATACATATC TAGTGAACAG 
GATGGAATGG AGCAGAAGAC AATTTTTTTA ATACCCTCTG ATTTAGCATG TAGACTGCTG 
GGGCAGTCAA TGGATGAGAG TGGATTACCA CAGCTGACCA GTTACGATTG TGAAGTTAAT 
GCTCCCATAC AAGGCAGCAG AAACCTACTG CAGGGTGAAG AATTACTCAG AGCTTTGGAT 
CAAGTTAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hif1a ( XM_006240196.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006240258.1 Rattus norvegicus hypoxia inducible factor 1 alpha subunit (Hif1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa29394
Clone ID Related Accession (Same CDS sequence)	XM_006240196.3
Accession Version	XM_006240196.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2487bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	hypoxia-inducible factor 1-alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005105.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006240196.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGCG CCGGCGGCGA GAACGAGAAG AAAAATAGGA TGAGTTCCGA ACGTCGAAAA 
GAAAAGTCTA GGGATGCAGC ACGATCTCGG CGAAGCAAAG AGTCTGAAGT TTTTTATGAG 
CTTGCTCATC AGTTGCCACT TCCCCACAAC GTGAGCTCCC ATCTTGATAA AGCTTCTGTT 
ATGAGGCTCA CCATCAGTTA CTTACGTGTG AGGAAACTTC TAGATGCTGG TGATCTTGAC 
ATTGAAGATG AAATGAAAGC ACAGATGAAC TGCTTTTATC TGAAAGCCCT GGATGGCTTT 
GTTATGGTGC TAACAGATGA TGGTGACATG ATTTACATTT CTGATAACGT GAACAAATAC 
ATGGGGTTGA CTCAGTTTGA ACTAACTGGA CACAGTGTGT TTGATTTTAC CCATCCATGT 
GACCATGAGG AAATGAGAGA AATGCTTACA CACAGAAATG GCCCAGTGAG AAAGGGGAAA 
GAACAAAACA CGCAGCGAAG CTTTTTTCTC AGAATGAAAT GTACCCTAAC AAGCCGGGGG 
AGGACGATGA ACATCAAGTC AGCAACGTGG AAGGTGCTGC ACTGCACAGG CCACATTCAT 
GTGTATGATA CCAGCAGTAA CCAGCCGCAG TGTGGCTACA AGAAACCGCC TATGACGTGC 
TTGGTGCTGA TTTGTGAACC CATTCCTCAT CCATCAAACA TTGAAATTCC TTTAGACAGC 
AAGACATTTC TCAGTCGACA CAGCCTCGAT ATGAAATTTT CTTACTGTGA TGAAAGGATT 
ACTGAGTTGA TGGGTTATGA GCCAGAAGAA CTTTTGGGCC GTTCAATTTA TGAATATTAT 
CATGCTTTGG ACTCTGATCA TCTGACCAAA ACTCATCATG ACATGTTTAC TAAAGGACAA 
GTCACCACAG GACAGTACAG GATGCTTGCA AAAAGAGGTG GATATGTCTG GGTTGAGACT 
CAAGCAACTG TTATATATAA TACGAAGAAC TCTCAGCCAC AGTGCATTGT GTGTGTGAAT 
TATGTTGTAA GTGGTATTAT TCAGCACGAC TTGATTTTCT CCCTTCAACA AACAGAATCT 
GTCCTCAAAC CAGTTGAATC TTCAGATATG AAAATGACCC AGCTGTTCAC TAAAGTGGAA 
TCTGAGGACA CGAGCTGCCT CTTCGACAAG CTTAAGAAAG AGCCCGATGC CCTGACTCTG 
CTAGCTCCAG CGGCTGGGGA CACGATCATA TCACTGGACT TCGGCAGCGA TGACACGGAA 
ACTGAAGACC AACAACTTGA AGATGTCCCG TTGTACAATG ATGTAATGTT CCCCTCTTCT 
AATGAGAAAT TAAATATAAA TCTGGCAATG TCTCCATTAC CTGCCTCTGA AACTCCAAAG 
CCACTTCGAA GTAGTGCTGA TCCTGCACTG AATCAAGAGG TTGCATTGAA GTTAGAGTCA 
AGCCCAGAGT CACTGGGACT TTCTTTTACC ATGCCCCAGA TTCAAGATCA GCCAGCAAGT 
CCTTCTGATG GAAGCACTAG ACAAAGCTCA CCTGAGCCTA ACAGTCCCAG TGAGTACTGC 
TTTGATGTGG ACAGCGATAT GGTCAATGTA TTCAAGTTGG AACTGGTGGA AAAACTGTTT 
GCTGAAGACA CAGAAGCGAA GAATCCATTT TCAGCTCAGG ACACTGATTT AGACTTGGAA 
ATGCTGGCTC CCTATATCCC AATGGATGAT GATTTCCAGT TACGTTCCTT TGATCAGTTG 
TCACCATTAG AGAGCAATTC TCCAAGCCCT CCGAGTGTGA GCACAGTTAC AGGATTCCAG 
CAGACCCAGT TACAGAAACC TACCATCACT GTCACTGCCG CAACTGCCAC CACCGCCACC 
ACCACTGATG AATCAAAAGC AGTGACGAAG GACAATATAG AAGACATTAA AATACTGATT 
GCATCTCCAC CTTCTACCCA AGTACCTCAA GAAATGACCA CTGCTAAGGC ATCAGCATAC 
AGTGGTACTC ACAGTCGGAC AACCTCACCA GACAGAGCAG GAAAGAGAGT CATAGAAAAA 
ACAGACAAAG CTCATCCAAG GAGCCTTAAC CTATCTGTCA CTTTGAATCA AAGAAATACT 
GTTCCTGAAG AAGAATTAAA CCCAAAGACA ATAGCTTTGC AGAATGCTCA GAGGAAGCGA 
AAAATGGAAC ATGATGGCTC CCTTTTTCAA GCAGCAGGAA TTGTAAGTGG AACGTTACTG 
CAGCAACCAG GTGACCGTGC CCCTACTATG TCGCTTTCTT GGAAACGAGT GAAAGGATAC 
ATATCTAGTG AACAGGATGG AATGGAGCAG AAGACAATTT TTTTAATACC CTCTGATTTA 
GCATGTAGAC TGCTGGGGCA GTCAATGGAT GAGAGTGGAT TACCACAGCT GACCAGTTAC 
GATTGTGAAG TTAATGCTCC CATACAAGGC AGCAGAAACC TACTGCAGGG TGAAGAATTA 
CTCAGAGCTT TGGATCAAGT TAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006240258.1
CDS	341..2827
Translation	MEGAGGENEKKNRMSSERRKEKSRDAARSRRSKESEVFYELAHQLPLPHNVSSHLDKASVMRLTISYLRVRKLLDAGDLDIEDEMKAQMNCFYLKALDGFVMVLTDDGDMIYISDNVNKYMGLTQFELTGHSVFDFTHPCDHEEMREMLTHRNGPVRKGKEQNTQRSFFLRMKCTLTSRGRTMNIKSATWKVLHCTGHIHVYDTSSNQPQCGYKKPPMTCLVLICEPIPHPSNIEIPLDSKTFLSRHSLDMKFSYCDERITELMGYEPEELLGRSIYEYYHALDSDHLTKTHHDMFTKGQVTTGQYRMLAKRGGYVWVETQATVIYNTKNSQPQCIVCVNYVVSGIIQHDLIFSLQQTESVLKPVESSDMKMTQLFTKVESEDTSCLFDKLKKEPDALTLLAPAAGDTIISLDFGSDDTETEDQQLEDVPLYNDVMFPSSNEKLNINLAMSPLPASETPKPLRSSADPALNQEVALKLESSPESLGLSFTMPQIQDQPASPSDGSTRQSSPEPNSPSEYCFDVDSDMVNVFKLELVEKLFAEDTEAKNPFSAQDTDLDLEMLAPYIPMDDDFQLRSFDQLSPLESNSPSPPSVSTVTGFQQTQLQKPTITVTAATATTATTTDESKAVTKDNIEDIKILIASPPSTQVPQEMTTAKASAYSGTHSRTTSPDRAGKRVIEKTDKAHPRSLNLSVTLNQRNTVPEEELNPKTIALQNAQRKRKMEHDGSLFQAAGIVSGTLLQQPGDRAPTMSLSWKRVKGYISSEQDGMEQKTIFLIPSDLACRLLGQSMDESGLPQLTSYDCEVNAPIQGSRNLLQGEELLRALDQVN

Target ORF information:

RefSeq Version	XM_006240196.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus hypoxia inducible factor 1 alpha subunit (Hif1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006240196.3

ORF Insert Sequence:

ATGGAGGGCG CCGGCGGCGA GAACGAGAAG AAAAATAGGA TGAGTTCCGA ACGTCGAAAA 
GAAAAGTCTA GGGATGCAGC ACGATCTCGG CGAAGCAAAG AGTCTGAAGT TTTTTATGAG 
CTTGCTCATC AGTTGCCACT TCCCCACAAC GTGAGCTCCC ATCTTGATAA AGCTTCTGTT 
ATGAGGCTCA CCATCAGTTA CTTACGTGTG AGGAAACTTC TAGATGCTGG TGATCTTGAC 
ATTGAAGATG AAATGAAAGC ACAGATGAAC TGCTTTTATC TGAAAGCCCT GGATGGCTTT 
GTTATGGTGC TAACAGATGA TGGTGACATG ATTTACATTT CTGATAACGT GAACAAATAC 
ATGGGGTTGA CTCAGTTTGA ACTAACTGGA CACAGTGTGT TTGATTTTAC CCATCCATGT 
GACCATGAGG AAATGAGAGA AATGCTTACA CACAGAAATG GCCCAGTGAG AAAGGGGAAA 
GAACAAAACA CGCAGCGAAG CTTTTTTCTC AGAATGAAAT GTACCCTAAC AAGCCGGGGG 
AGGACGATGA ACATCAAGTC AGCAACGTGG AAGGTGCTGC ACTGCACAGG CCACATTCAT 
GTGTATGATA CCAGCAGTAA CCAGCCGCAG TGTGGCTACA AGAAACCGCC TATGACGTGC 
TTGGTGCTGA TTTGTGAACC CATTCCTCAT CCATCAAACA TTGAAATTCC TTTAGACAGC 
AAGACATTTC TCAGTCGACA CAGCCTCGAT ATGAAATTTT CTTACTGTGA TGAAAGGATT 
ACTGAGTTGA TGGGTTATGA GCCAGAAGAA CTTTTGGGCC GTTCAATTTA TGAATATTAT 
CATGCTTTGG ACTCTGATCA TCTGACCAAA ACTCATCATG ACATGTTTAC TAAAGGACAA 
GTCACCACAG GACAGTACAG GATGCTTGCA AAAAGAGGTG GATATGTCTG GGTTGAGACT 
CAAGCAACTG TTATATATAA TACGAAGAAC TCTCAGCCAC AGTGCATTGT GTGTGTGAAT 
TATGTTGTAA GTGGTATTAT TCAGCACGAC TTGATTTTCT CCCTTCAACA AACAGAATCT 
GTCCTCAAAC CAGTTGAATC TTCAGATATG AAAATGACCC AGCTGTTCAC TAAAGTGGAA 
TCTGAGGACA CGAGCTGCCT CTTCGACAAG CTTAAGAAAG AGCCCGATGC CCTGACTCTG 
CTAGCTCCAG CGGCTGGGGA CACGATCATA TCACTGGACT TCGGCAGCGA TGACACGGAA 
ACTGAAGACC AACAACTTGA AGATGTCCCG TTGTACAATG ATGTAATGTT CCCCTCTTCT 
AATGAGAAAT TAAATATAAA TCTGGCAATG TCTCCATTAC CTGCCTCTGA AACTCCAAAG 
CCACTTCGAA GTAGTGCTGA TCCTGCACTG AATCAAGAGG TTGCATTGAA GTTAGAGTCA 
AGCCCAGAGT CACTGGGACT TTCTTTTACC ATGCCCCAGA TTCAAGATCA GCCAGCAAGT 
CCTTCTGATG GAAGCACTAG ACAAAGCTCA CCTGAGCCTA ACAGTCCCAG TGAGTACTGC 
TTTGATGTGG ACAGCGATAT GGTCAATGTA TTCAAGTTGG AACTGGTGGA AAAACTGTTT 
GCTGAAGACA CAGAAGCGAA GAATCCATTT TCAGCTCAGG ACACTGATTT AGACTTGGAA 
ATGCTGGCTC CCTATATCCC AATGGATGAT GATTTCCAGT TACGTTCCTT TGATCAGTTG 
TCACCATTAG AGAGCAATTC TCCAAGCCCT CCGAGTGTGA GCACAGTTAC AGGATTCCAG 
CAGACCCAGT TACAGAAACC TACCATCACT GTCACTGCCG CAACTGCCAC CACCGCCACC 
ACCACTGATG AATCAAAAGC AGTGACGAAG GACAATATAG AAGACATTAA AATACTGATT 
GCATCTCCAC CTTCTACCCA AGTACCTCAA GAAATGACCA CTGCTAAGGC ATCAGCATAC 
AGTGGTACTC ACAGTCGGAC AACCTCACCA GACAGAGCAG GAAAGAGAGT CATAGAAAAA 
ACAGACAAAG CTCATCCAAG GAGCCTTAAC CTATCTGTCA CTTTGAATCA AAGAAATACT 
GTTCCTGAAG AAGAATTAAA CCCAAAGACA ATAGCTTTGC AGAATGCTCA GAGGAAGCGA 
AAAATGGAAC ATGATGGCTC CCTTTTTCAA GCAGCAGGAA TTGTAAGTGG AACGTTACTG 
CAGCAACCAG GTGACCGTGC CCCTACTATG TCGCTTTCTT GGAAACGAGT GAAAGGATAC 
ATATCTAGTG AACAGGATGG AATGGAGCAG AAGACAATTT TTTTAATACC CTCTGATTTA 
GCATGTAGAC TGCTGGGGCA GTCAATGGAT GAGAGTGGAT TACCACAGCT GACCAGTTAC 
GATTGTGAAG TTAATGCTCC CATACAAGGC AGCAGAAACC TACTGCAGGG TGAAGAATTA 
CTCAGAGCTT TGGATCAAGT TAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hif1a ( XM_006240197.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006240259.1 Rattus norvegicus hypoxia inducible factor 1 alpha subunit (Hif1a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa29395
Clone ID Related Accession (Same CDS sequence)	XM_006240197.3
Accession Version	XM_006240197.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2484bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	hypoxia-inducible factor 1-alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005105.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006240197.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGCG CCGGCGGCGA GAACGAGAAG AAAAAGATGA GTTCCGAACG TCGAAAAGAA 
AAGTCTAGGG ATGCAGCACG ATCTCGGCGA AGCAAAGAGT CTGAAGTTTT TTATGAGCTT 
GCTCATCAGT TGCCACTTCC CCACAACGTG AGCTCCCATC TTGATAAAGC TTCTGTTATG 
AGGCTCACCA TCAGTTACTT ACGTGTGAGG AAACTTCTAG ATGCTGGTGA TCTTGACATT 
GAAGATGAAA TGAAAGCACA GATGAACTGC TTTTATCTGA AAGCCCTGGA TGGCTTTGTT 
ATGGTGCTAA CAGATGATGG TGACATGATT TACATTTCTG ATAACGTGAA CAAATACATG 
GGGTTGACTC AGTTTGAACT AACTGGACAC AGTGTGTTTG ATTTTACCCA TCCATGTGAC 
CATGAGGAAA TGAGAGAAAT GCTTACACAC AGAAATGGCC CAGTGAGAAA GGGGAAAGAA 
CAAAACACGC AGCGAAGCTT TTTTCTCAGA ATGAAATGTA CCCTAACAAG CCGGGGGAGG 
ACGATGAACA TCAAGTCAGC AACGTGGAAG GTGCTGCACT GCACAGGCCA CATTCATGTG 
TATGATACCA GCAGTAACCA GCCGCAGTGT GGCTACAAGA AACCGCCTAT GACGTGCTTG 
GTGCTGATTT GTGAACCCAT TCCTCATCCA TCAAACATTG AAATTCCTTT AGACAGCAAG 
ACATTTCTCA GTCGACACAG CCTCGATATG AAATTTTCTT ACTGTGATGA AAGGATTACT 
GAGTTGATGG GTTATGAGCC AGAAGAACTT TTGGGCCGTT CAATTTATGA ATATTATCAT 
GCTTTGGACT CTGATCATCT GACCAAAACT CATCATGACA TGTTTACTAA AGGACAAGTC 
ACCACAGGAC AGTACAGGAT GCTTGCAAAA AGAGGTGGAT ATGTCTGGGT TGAGACTCAA 
GCAACTGTTA TATATAATAC GAAGAACTCT CAGCCACAGT GCATTGTGTG TGTGAATTAT 
GTTGTAAGTG GTATTATTCA GCACGACTTG ATTTTCTCCC TTCAACAAAC AGAATCTGTC 
CTCAAACCAG TTGAATCTTC AGATATGAAA ATGACCCAGC TGTTCACTAA AGTGGAATCT 
GAGGACACGA GCTGCCTCTT CGACAAGCTT AAGAAAGAGC CCGATGCCCT GACTCTGCTA 
GCTCCAGCGG CTGGGGACAC GATCATATCA CTGGACTTCG GCAGCGATGA CACGGAAACT 
GAAGACCAAC AACTTGAAGA TGTCCCGTTG TACAATGATG TAATGTTCCC CTCTTCTAAT 
GAGAAATTAA ATATAAATCT GGCAATGTCT CCATTACCTG CCTCTGAAAC TCCAAAGCCA 
CTTCGAAGTA GTGCTGATCC TGCACTGAAT CAAGAGGTTG CATTGAAGTT AGAGTCAAGC 
CCAGAGTCAC TGGGACTTTC TTTTACCATG CCCCAGATTC AAGATCAGCC AGCAAGTCCT 
TCTGATGGAA GCACTAGACA AAGCTCACCT GAGCCTAACA GTCCCAGTGA GTACTGCTTT 
GATGTGGACA GCGATATGGT CAATGTATTC AAGTTGGAAC TGGTGGAAAA ACTGTTTGCT 
GAAGACACAG AAGCGAAGAA TCCATTTTCA GCTCAGGACA CTGATTTAGA CTTGGAAATG 
CTGGCTCCCT ATATCCCAAT GGATGATGAT TTCCAGTTAC GTTCCTTTGA TCAGTTGTCA 
CCATTAGAGA GCAATTCTCC AAGCCCTCCG AGTGTGAGCA CAGTTACAGG ATTCCAGCAG 
ACCCAGTTAC AGAAACCTAC CATCACTGTC ACTGCCGCAA CTGCCACCAC CGCCACCACC 
ACTGATGAAT CAAAAGCAGT GACGAAGGAC AATATAGAAG ACATTAAAAT ACTGATTGCA 
TCTCCACCTT CTACCCAAGT ACCTCAAGAA ATGACCACTG CTAAGGCATC AGCATACAGT 
GGTACTCACA GTCGGACAAC CTCACCAGAC AGAGCAGGAA AGAGAGTCAT AGAAAAAACA 
GACAAAGCTC ATCCAAGGAG CCTTAACCTA TCTGTCACTT TGAATCAAAG AAATACTGTT 
CCTGAAGAAG AATTAAACCC AAAGACAATA GCTTTGCAGA ATGCTCAGAG GAAGCGAAAA 
ATGGAACATG ATGGCTCCCT TTTTCAAGCA GCAGGAATTG TAAGTGGAAC GTTACTGCAG 
CAACCAGGTG ACCGTGCCCC TACTATGTCG CTTTCTTGGA AACGAGTGAA AGGATACATA 
TCTAGTGAAC AGGATGGAAT GGAGCAGAAG ACAATTTTTT TAATACCCTC TGATTTAGCA 
TGTAGACTGC TGGGGCAGTC AATGGATGAG AGTGGATTAC CACAGCTGAC CAGTTACGAT 
TGTGAAGTTA ATGCTCCCAT ACAAGGCAGC AGAAACCTAC TGCAGGGTGA AGAATTACTC 
AGAGCTTTGG ATCAAGTTAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006240259.1
CDS	341..2824
Translation	MEGAGGENEKKKMSSERRKEKSRDAARSRRSKESEVFYELAHQLPLPHNVSSHLDKASVMRLTISYLRVRKLLDAGDLDIEDEMKAQMNCFYLKALDGFVMVLTDDGDMIYISDNVNKYMGLTQFELTGHSVFDFTHPCDHEEMREMLTHRNGPVRKGKEQNTQRSFFLRMKCTLTSRGRTMNIKSATWKVLHCTGHIHVYDTSSNQPQCGYKKPPMTCLVLICEPIPHPSNIEIPLDSKTFLSRHSLDMKFSYCDERITELMGYEPEELLGRSIYEYYHALDSDHLTKTHHDMFTKGQVTTGQYRMLAKRGGYVWVETQATVIYNTKNSQPQCIVCVNYVVSGIIQHDLIFSLQQTESVLKPVESSDMKMTQLFTKVESEDTSCLFDKLKKEPDALTLLAPAAGDTIISLDFGSDDTETEDQQLEDVPLYNDVMFPSSNEKLNINLAMSPLPASETPKPLRSSADPALNQEVALKLESSPESLGLSFTMPQIQDQPASPSDGSTRQSSPEPNSPSEYCFDVDSDMVNVFKLELVEKLFAEDTEAKNPFSAQDTDLDLEMLAPYIPMDDDFQLRSFDQLSPLESNSPSPPSVSTVTGFQQTQLQKPTITVTAATATTATTTDESKAVTKDNIEDIKILIASPPSTQVPQEMTTAKASAYSGTHSRTTSPDRAGKRVIEKTDKAHPRSLNLSVTLNQRNTVPEEELNPKTIALQNAQRKRKMEHDGSLFQAAGIVSGTLLQQPGDRAPTMSLSWKRVKGYISSEQDGMEQKTIFLIPSDLACRLLGQSMDESGLPQLTSYDCEVNAPIQGSRNLLQGEELLRALDQVN

Target ORF information:

RefSeq Version	XM_006240197.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus hypoxia inducible factor 1 alpha subunit (Hif1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006240197.3

ORF Insert Sequence:

ATGGAGGGCG CCGGCGGCGA GAACGAGAAG AAAAAGATGA GTTCCGAACG TCGAAAAGAA 
AAGTCTAGGG ATGCAGCACG ATCTCGGCGA AGCAAAGAGT CTGAAGTTTT TTATGAGCTT 
GCTCATCAGT TGCCACTTCC CCACAACGTG AGCTCCCATC TTGATAAAGC TTCTGTTATG 
AGGCTCACCA TCAGTTACTT ACGTGTGAGG AAACTTCTAG ATGCTGGTGA TCTTGACATT 
GAAGATGAAA TGAAAGCACA GATGAACTGC TTTTATCTGA AAGCCCTGGA TGGCTTTGTT 
ATGGTGCTAA CAGATGATGG TGACATGATT TACATTTCTG ATAACGTGAA CAAATACATG 
GGGTTGACTC AGTTTGAACT AACTGGACAC AGTGTGTTTG ATTTTACCCA TCCATGTGAC 
CATGAGGAAA TGAGAGAAAT GCTTACACAC AGAAATGGCC CAGTGAGAAA GGGGAAAGAA 
CAAAACACGC AGCGAAGCTT TTTTCTCAGA ATGAAATGTA CCCTAACAAG CCGGGGGAGG 
ACGATGAACA TCAAGTCAGC AACGTGGAAG GTGCTGCACT GCACAGGCCA CATTCATGTG 
TATGATACCA GCAGTAACCA GCCGCAGTGT GGCTACAAGA AACCGCCTAT GACGTGCTTG 
GTGCTGATTT GTGAACCCAT TCCTCATCCA TCAAACATTG AAATTCCTTT AGACAGCAAG 
ACATTTCTCA GTCGACACAG CCTCGATATG AAATTTTCTT ACTGTGATGA AAGGATTACT 
GAGTTGATGG GTTATGAGCC AGAAGAACTT TTGGGCCGTT CAATTTATGA ATATTATCAT 
GCTTTGGACT CTGATCATCT GACCAAAACT CATCATGACA TGTTTACTAA AGGACAAGTC 
ACCACAGGAC AGTACAGGAT GCTTGCAAAA AGAGGTGGAT ATGTCTGGGT TGAGACTCAA 
GCAACTGTTA TATATAATAC GAAGAACTCT CAGCCACAGT GCATTGTGTG TGTGAATTAT 
GTTGTAAGTG GTATTATTCA GCACGACTTG ATTTTCTCCC TTCAACAAAC AGAATCTGTC 
CTCAAACCAG TTGAATCTTC AGATATGAAA ATGACCCAGC TGTTCACTAA AGTGGAATCT 
GAGGACACGA GCTGCCTCTT CGACAAGCTT AAGAAAGAGC CCGATGCCCT GACTCTGCTA 
GCTCCAGCGG CTGGGGACAC GATCATATCA CTGGACTTCG GCAGCGATGA CACGGAAACT 
GAAGACCAAC AACTTGAAGA TGTCCCGTTG TACAATGATG TAATGTTCCC CTCTTCTAAT 
GAGAAATTAA ATATAAATCT GGCAATGTCT CCATTACCTG CCTCTGAAAC TCCAAAGCCA 
CTTCGAAGTA GTGCTGATCC TGCACTGAAT CAAGAGGTTG CATTGAAGTT AGAGTCAAGC 
CCAGAGTCAC TGGGACTTTC TTTTACCATG CCCCAGATTC AAGATCAGCC AGCAAGTCCT 
TCTGATGGAA GCACTAGACA AAGCTCACCT GAGCCTAACA GTCCCAGTGA GTACTGCTTT 
GATGTGGACA GCGATATGGT CAATGTATTC AAGTTGGAAC TGGTGGAAAA ACTGTTTGCT 
GAAGACACAG AAGCGAAGAA TCCATTTTCA GCTCAGGACA CTGATTTAGA CTTGGAAATG 
CTGGCTCCCT ATATCCCAAT GGATGATGAT TTCCAGTTAC GTTCCTTTGA TCAGTTGTCA 
CCATTAGAGA GCAATTCTCC AAGCCCTCCG AGTGTGAGCA CAGTTACAGG ATTCCAGCAG 
ACCCAGTTAC AGAAACCTAC CATCACTGTC ACTGCCGCAA CTGCCACCAC CGCCACCACC 
ACTGATGAAT CAAAAGCAGT GACGAAGGAC AATATAGAAG ACATTAAAAT ACTGATTGCA 
TCTCCACCTT CTACCCAAGT ACCTCAAGAA ATGACCACTG CTAAGGCATC AGCATACAGT 
GGTACTCACA GTCGGACAAC CTCACCAGAC AGAGCAGGAA AGAGAGTCAT AGAAAAAACA 
GACAAAGCTC ATCCAAGGAG CCTTAACCTA TCTGTCACTT TGAATCAAAG AAATACTGTT 
CCTGAAGAAG AATTAAACCC AAAGACAATA GCTTTGCAGA ATGCTCAGAG GAAGCGAAAA 
ATGGAACATG ATGGCTCCCT TTTTCAAGCA GCAGGAATTG TAAGTGGAAC GTTACTGCAG 
CAACCAGGTG ACCGTGCCCC TACTATGTCG CTTTCTTGGA AACGAGTGAA AGGATACATA 
TCTAGTGAAC AGGATGGAAT GGAGCAGAAG ACAATTTTTT TAATACCCTC TGATTTAGCA 
TGTAGACTGC TGGGGCAGTC AATGGATGAG AGTGGATTAC CACAGCTGAC CAGTTACGAT 
TGTGAAGTTA ATGCTCCCAT ACAAGGCAGC AGAAACCTAC TGCAGGGTGA AGAATTACTC 
AGAGCTTTGG ATCAAGTTAA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hif1a ( XM_006240198.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006240260.1 Rattus norvegicus hypoxia inducible factor 1 alpha subunit (Hif1a), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa29396
Clone ID Related Accession (Same CDS sequence)	XM_006240198.3
Accession Version	XM_006240198.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2478bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	hypoxia-inducible factor 1-alpha isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005105.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006240198.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGGCG CCGGCGGCGA GAACGAGAAG AAAAAGATGA GTTCCGAACG TCGAAAAGAA 
AAGTCTAGGG ATGCAGCACG ATCTCGGCGA AGCAAAGAGT CTGAAGTTTT TTATGAGCTT 
GCTCATCAGT TGCCACTTCC CCACAACGTG AGCTCCCATC TTGATAAAGC TTCTGTTATG 
AGGCTCACCA TCAGTTACTT ACGTGTGAGG AAACTTCTAG ATGCTGGTGA TCTTGACATT 
GAAGATGAAA TGAAAGCACA GATGAACTGC TTTTATCTGA AAGCCCTGGA TGGCTTTGTT 
ATGGTGCTAA CAGATGATGG TGACATGATT TACATTTCTG ATAACGTGAA CAAATACATG 
GGGTTGACTC AGTTTGAACT AACTGGACAC AGTGTGTTTG ATTTTACCCA TCCATGTGAC 
CATGAGGAAA TGAGAGAAAT GCTTACACAC AGAAATGGCC CAGTGAGAAA GGGGAAAGAA 
CAAAACACGC AGCGAAGCTT TTTTCTCAGA ATGAAATGTA CCCTAACAAG CCGGGGGAGG 
ACGATGAACA TCAAGTCAGC AACGTGGAAG GTGCTGCACT GCACAGGCCA CATTCATGTG 
TATGATACCA GCAGTAACCA GCCGCAGTGT GGCTACAAGA AACCGCCTAT GACGTGCTTG 
GTGCTGATTT GTGAACCCAT TCCTCATCCA TCAAACATTG AAATTCCTTT AGACAGCAAG 
ACATTTCTCA GTCGACACAG CCTCGATATG AAATTTTCTT ACTGTGATGA AAGGATTACT 
GAGTTGATGG GTTATGAGCC AGAAGAACTT TTGGGCCGTT CAATTTATGA ATATTATCAT 
GCTTTGGACT CTGATCATCT GACCAAAACT CATCATGACA TGTTTACTAA AGGACAAGTC 
ACCACAGGAC AGTACAGGAT GCTTGCAAAA AGAGGTGGAT ATGTCTGGGT TGAGACTCAA 
GCAACTGTTA TATATAATAC GAAGAACTCT CAGCCACAGT GCATTGTGTG TGTGAATTAT 
GTTGTAAGTG GTATTATTCA GCACGACTTG ATTTTCTCCC TTCAACAAAC AGAATCTGTC 
CTCAAACCAG TTGAATCTTC AGATATGAAA ATGACCCAGC TGTTCACTAA AGTGGAATCT 
GAGGACACGA GCTGCCTCTT CGACAAGCTT AAGAAAGAGC CCGATGCCCT GACTCTGCTA 
GCTCCAGCGG CTGGGGACAC GATCATATCA CTGGACTTCG GCAGCGATGA CACGGAAACT 
GAAGACCAAC AACTTGAAGA TGTCCCGTTG TACAATGATG TAATGTTCCC CTCTTCTAAT 
GAGAAATTAA ATATAAATCT GGCAATGTCT CCATTACCTG CCTCTGAAAC TCCAAAGCCA 
CTTCGAAGTA GTGCTGATCC TGCACTGAAT CAAGAGGTTG CATTGAAGTT AGAGTCAAGC 
CCAGAGTCAC TGGGACTTTC TTTTACCATG CCCCAGATTC AAGATCAGCC AGCAAGTCCT 
TCTGATGGAA GCACTAGACA AAGCTCACCT GAGCCTAACA GTCCCAGTGA GTACTGCTTT 
GATGTGGACA GCGATATGGT CAATGTATTC AAGTTGGAAC TGGTGGAAAA ACTGTTTGCT 
GAAGACACAG AAGCGAAGAA TCCATTTTCA GCTCAGGACA CTGATTTAGA CTTGGAAATG 
CTGGCTCCCT ATATCCCAAT GGATGATGAT TTCCAGTTAC GTTCCTTTGA TCAGTTGTCA 
CCATTAGAGA GCAATTCTCC AAGCCCTCCG AGTGTGAGCA CAGTTACAGG ATTCCAGCAG 
ACCCAGTTAC AGAAACCTAC CATCACTGTC ACTGCCGCAA CTGCCACCAC CGCCACCACC 
ACTGATGAAT CAAAAGCAGT GACGAAGGAC AATATAGAAG ACATTAAAAT ACTGATTGCA 
TCTCCACCTT CTACCCAAGT ACCTCAAGAA ATGACCACTG CTAAGGCATC AGCATACAGT 
GGTACTCACA GTCGGACAAC CTCACCAGAC AGAGCAGGAA AGAGAGTCAT AGAAAAAACA 
GACAAAGCTC ATCCAAGGAG CCTTAACCTA TCTGTCACTT TGAATCAAAG AAATACTGTT 
CCTGAAGAAG AATTAAACCC AAAGACAATA GCTTTGCAGA ATGCTCAGAG GAAGCGAAAA 
ATGGAACATG ATGGCTCCCT TTTTCAAGCA GCAGGAATTG GAACGTTACT GCAGCAACCA 
GGTGACCGTG CCCCTACTAT GTCGCTTTCT TGGAAACGAG TGAAAGGATA CATATCTAGT 
GAACAGGATG GAATGGAGCA GAAGACAATT TTTTTAATAC CCTCTGATTT AGCATGTAGA 
CTGCTGGGGC AGTCAATGGA TGAGAGTGGA TTACCACAGC TGACCAGTTA CGATTGTGAA 
GTTAATGCTC CCATACAAGG CAGCAGAAAC CTACTGCAGG GTGAAGAATT ACTCAGAGCT 
TTGGATCAAG TTAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006240260.1
CDS	86..2563
Translation	MEGAGGENEKKKMSSERRKEKSRDAARSRRSKESEVFYELAHQLPLPHNVSSHLDKASVMRLTISYLRVRKLLDAGDLDIEDEMKAQMNCFYLKALDGFVMVLTDDGDMIYISDNVNKYMGLTQFELTGHSVFDFTHPCDHEEMREMLTHRNGPVRKGKEQNTQRSFFLRMKCTLTSRGRTMNIKSATWKVLHCTGHIHVYDTSSNQPQCGYKKPPMTCLVLICEPIPHPSNIEIPLDSKTFLSRHSLDMKFSYCDERITELMGYEPEELLGRSIYEYYHALDSDHLTKTHHDMFTKGQVTTGQYRMLAKRGGYVWVETQATVIYNTKNSQPQCIVCVNYVVSGIIQHDLIFSLQQTESVLKPVESSDMKMTQLFTKVESEDTSCLFDKLKKEPDALTLLAPAAGDTIISLDFGSDDTETEDQQLEDVPLYNDVMFPSSNEKLNINLAMSPLPASETPKPLRSSADPALNQEVALKLESSPESLGLSFTMPQIQDQPASPSDGSTRQSSPEPNSPSEYCFDVDSDMVNVFKLELVEKLFAEDTEAKNPFSAQDTDLDLEMLAPYIPMDDDFQLRSFDQLSPLESNSPSPPSVSTVTGFQQTQLQKPTITVTAATATTATTTDESKAVTKDNIEDIKILIASPPSTQVPQEMTTAKASAYSGTHSRTTSPDRAGKRVIEKTDKAHPRSLNLSVTLNQRNTVPEEELNPKTIALQNAQRKRKMEHDGSLFQAAGIGTLLQQPGDRAPTMSLSWKRVKGYISSEQDGMEQKTIFLIPSDLACRLLGQSMDESGLPQLTSYDCEVNAPIQGSRNLLQGEELLRALDQVN

Target ORF information:

RefSeq Version	XM_006240198.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus hypoxia inducible factor 1 alpha subunit (Hif1a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006240198.3

ORF Insert Sequence:

ATGGAGGGCG CCGGCGGCGA GAACGAGAAG AAAAAGATGA GTTCCGAACG TCGAAAAGAA 
AAGTCTAGGG ATGCAGCACG ATCTCGGCGA AGCAAAGAGT CTGAAGTTTT TTATGAGCTT 
GCTCATCAGT TGCCACTTCC CCACAACGTG AGCTCCCATC TTGATAAAGC TTCTGTTATG 
AGGCTCACCA TCAGTTACTT ACGTGTGAGG AAACTTCTAG ATGCTGGTGA TCTTGACATT 
GAAGATGAAA TGAAAGCACA GATGAACTGC TTTTATCTGA AAGCCCTGGA TGGCTTTGTT 
ATGGTGCTAA CAGATGATGG TGACATGATT TACATTTCTG ATAACGTGAA CAAATACATG 
GGGTTGACTC AGTTTGAACT AACTGGACAC AGTGTGTTTG ATTTTACCCA TCCATGTGAC 
CATGAGGAAA TGAGAGAAAT GCTTACACAC AGAAATGGCC CAGTGAGAAA GGGGAAAGAA 
CAAAACACGC AGCGAAGCTT TTTTCTCAGA ATGAAATGTA CCCTAACAAG CCGGGGGAGG 
ACGATGAACA TCAAGTCAGC AACGTGGAAG GTGCTGCACT GCACAGGCCA CATTCATGTG 
TATGATACCA GCAGTAACCA GCCGCAGTGT GGCTACAAGA AACCGCCTAT GACGTGCTTG 
GTGCTGATTT GTGAACCCAT TCCTCATCCA TCAAACATTG AAATTCCTTT AGACAGCAAG 
ACATTTCTCA GTCGACACAG CCTCGATATG AAATTTTCTT ACTGTGATGA AAGGATTACT 
GAGTTGATGG GTTATGAGCC AGAAGAACTT TTGGGCCGTT CAATTTATGA ATATTATCAT 
GCTTTGGACT CTGATCATCT GACCAAAACT CATCATGACA TGTTTACTAA AGGACAAGTC 
ACCACAGGAC AGTACAGGAT GCTTGCAAAA AGAGGTGGAT ATGTCTGGGT TGAGACTCAA 
GCAACTGTTA TATATAATAC GAAGAACTCT CAGCCACAGT GCATTGTGTG TGTGAATTAT 
GTTGTAAGTG GTATTATTCA GCACGACTTG ATTTTCTCCC TTCAACAAAC AGAATCTGTC 
CTCAAACCAG TTGAATCTTC AGATATGAAA ATGACCCAGC TGTTCACTAA AGTGGAATCT 
GAGGACACGA GCTGCCTCTT CGACAAGCTT AAGAAAGAGC CCGATGCCCT GACTCTGCTA 
GCTCCAGCGG CTGGGGACAC GATCATATCA CTGGACTTCG GCAGCGATGA CACGGAAACT 
GAAGACCAAC AACTTGAAGA TGTCCCGTTG TACAATGATG TAATGTTCCC CTCTTCTAAT 
GAGAAATTAA ATATAAATCT GGCAATGTCT CCATTACCTG CCTCTGAAAC TCCAAAGCCA 
CTTCGAAGTA GTGCTGATCC TGCACTGAAT CAAGAGGTTG CATTGAAGTT AGAGTCAAGC 
CCAGAGTCAC TGGGACTTTC TTTTACCATG CCCCAGATTC AAGATCAGCC AGCAAGTCCT 
TCTGATGGAA GCACTAGACA AAGCTCACCT GAGCCTAACA GTCCCAGTGA GTACTGCTTT 
GATGTGGACA GCGATATGGT CAATGTATTC AAGTTGGAAC TGGTGGAAAA ACTGTTTGCT 
GAAGACACAG AAGCGAAGAA TCCATTTTCA GCTCAGGACA CTGATTTAGA CTTGGAAATG 
CTGGCTCCCT ATATCCCAAT GGATGATGAT TTCCAGTTAC GTTCCTTTGA TCAGTTGTCA 
CCATTAGAGA GCAATTCTCC AAGCCCTCCG AGTGTGAGCA CAGTTACAGG ATTCCAGCAG 
ACCCAGTTAC AGAAACCTAC CATCACTGTC ACTGCCGCAA CTGCCACCAC CGCCACCACC 
ACTGATGAAT CAAAAGCAGT GACGAAGGAC AATATAGAAG ACATTAAAAT ACTGATTGCA 
TCTCCACCTT CTACCCAAGT ACCTCAAGAA ATGACCACTG CTAAGGCATC AGCATACAGT 
GGTACTCACA GTCGGACAAC CTCACCAGAC AGAGCAGGAA AGAGAGTCAT AGAAAAAACA 
GACAAAGCTC ATCCAAGGAG CCTTAACCTA TCTGTCACTT TGAATCAAAG AAATACTGTT 
CCTGAAGAAG AATTAAACCC AAAGACAATA GCTTTGCAGA ATGCTCAGAG GAAGCGAAAA 
ATGGAACATG ATGGCTCCCT TTTTCAAGCA GCAGGAATTG GAACGTTACT GCAGCAACCA 
GGTGACCGTG CCCCTACTAT GTCGCTTTCT TGGAAACGAG TGAAAGGATA CATATCTAGT 
GAACAGGATG GAATGGAGCA GAAGACAATT TTTTTAATAC CCTCTGATTT AGCATGTAGA 
CTGCTGGGGC AGTCAATGGA TGAGAGTGGA TTACCACAGC TGACCAGTTA CGATTGTGAA 
GTTAATGCTC CCATACAAGG CAGCAGAAAC CTACTGCAGG GTGAAGAATT ACTCAGAGCT 
TTGGATCAAG TTAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hif1a ( XM_006240199.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006240261.1 Rattus norvegicus hypoxia inducible factor 1 alpha subunit (Hif1a), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa29397
Clone ID Related Accession (Same CDS sequence)	XM_006240199.3
Accession Version	XM_006240199.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2448bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	hypoxia-inducible factor 1-alpha isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005105.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006240199.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTCCG AACGTCGAAA AGAAAAGTCT AGGGATGCAG CACGATCTCG GCGAAGCAAA 
GAGTCTGAAG TTTTTTATGA GCTTGCTCAT CAGTTGCCAC TTCCCCACAA CGTGAGCTCC 
CATCTTGATA AAGCTTCTGT TATGAGGCTC ACCATCAGTT ACTTACGTGT GAGGAAACTT 
CTAGATGCTG GTGATCTTGA CATTGAAGAT GAAATGAAAG CACAGATGAA CTGCTTTTAT 
CTGAAAGCCC TGGATGGCTT TGTTATGGTG CTAACAGATG ATGGTGACAT GATTTACATT 
TCTGATAACG TGAACAAATA CATGGGGTTG ACTCAGTTTG AACTAACTGG ACACAGTGTG 
TTTGATTTTA CCCATCCATG TGACCATGAG GAAATGAGAG AAATGCTTAC ACACAGAAAT 
GGCCCAGTGA GAAAGGGGAA AGAACAAAAC ACGCAGCGAA GCTTTTTTCT CAGAATGAAA 
TGTACCCTAA CAAGCCGGGG GAGGACGATG AACATCAAGT CAGCAACGTG GAAGGTGCTG 
CACTGCACAG GCCACATTCA TGTGTATGAT ACCAGCAGTA ACCAGCCGCA GTGTGGCTAC 
AAGAAACCGC CTATGACGTG CTTGGTGCTG ATTTGTGAAC CCATTCCTCA TCCATCAAAC 
ATTGAAATTC CTTTAGACAG CAAGACATTT CTCAGTCGAC ACAGCCTCGA TATGAAATTT 
TCTTACTGTG ATGAAAGGAT TACTGAGTTG ATGGGTTATG AGCCAGAAGA ACTTTTGGGC 
CGTTCAATTT ATGAATATTA TCATGCTTTG GACTCTGATC ATCTGACCAA AACTCATCAT 
GACATGTTTA CTAAAGGACA AGTCACCACA GGACAGTACA GGATGCTTGC AAAAAGAGGT 
GGATATGTCT GGGTTGAGAC TCAAGCAACT GTTATATATA ATACGAAGAA CTCTCAGCCA 
CAGTGCATTG TGTGTGTGAA TTATGTTGTA AGTGGTATTA TTCAGCACGA CTTGATTTTC 
TCCCTTCAAC AAACAGAATC TGTCCTCAAA CCAGTTGAAT CTTCAGATAT GAAAATGACC 
CAGCTGTTCA CTAAAGTGGA ATCTGAGGAC ACGAGCTGCC TCTTCGACAA GCTTAAGAAA 
GAGCCCGATG CCCTGACTCT GCTAGCTCCA GCGGCTGGGG ACACGATCAT ATCACTGGAC 
TTCGGCAGCG ATGACACGGA AACTGAAGAC CAACAACTTG AAGATGTCCC GTTGTACAAT 
GATGTAATGT TCCCCTCTTC TAATGAGAAA TTAAATATAA ATCTGGCAAT GTCTCCATTA 
CCTGCCTCTG AAACTCCAAA GCCACTTCGA AGTAGTGCTG ATCCTGCACT GAATCAAGAG 
GTTGCATTGA AGTTAGAGTC AAGCCCAGAG TCACTGGGAC TTTCTTTTAC CATGCCCCAG 
ATTCAAGATC AGCCAGCAAG TCCTTCTGAT GGAAGCACTA GACAAAGCTC ACCTGAGCCT 
AACAGTCCCA GTGAGTACTG CTTTGATGTG GACAGCGATA TGGTCAATGT ATTCAAGTTG 
GAACTGGTGG AAAAACTGTT TGCTGAAGAC ACAGAAGCGA AGAATCCATT TTCAGCTCAG 
GACACTGATT TAGACTTGGA AATGCTGGCT CCCTATATCC CAATGGATGA TGATTTCCAG 
TTACGTTCCT TTGATCAGTT GTCACCATTA GAGAGCAATT CTCCAAGCCC TCCGAGTGTG 
AGCACAGTTA CAGGATTCCA GCAGACCCAG TTACAGAAAC CTACCATCAC TGTCACTGCC 
GCAACTGCCA CCACCGCCAC CACCACTGAT GAATCAAAAG CAGTGACGAA GGACAATATA 
GAAGACATTA AAATACTGAT TGCATCTCCA CCTTCTACCC AAGTACCTCA AGAAATGACC 
ACTGCTAAGG CATCAGCATA CAGTGGTACT CACAGTCGGA CAACCTCACC AGACAGAGCA 
GGAAAGAGAG TCATAGAAAA AACAGACAAA GCTCATCCAA GGAGCCTTAA CCTATCTGTC 
ACTTTGAATC AAAGAAATAC TGTTCCTGAA GAAGAATTAA ACCCAAAGAC AATAGCTTTG 
CAGAATGCTC AGAGGAAGCG AAAAATGGAA CATGATGGCT CCCTTTTTCA AGCAGCAGGA 
ATTGTAAGTG GAACGTTACT GCAGCAACCA GGTGACCGTG CCCCTACTAT GTCGCTTTCT 
TGGAAACGAG TGAAAGGATA CATATCTAGT GAACAGGATG GAATGGAGCA GAAGACAATT 
TTTTTAATAC CCTCTGATTT AGCATGTAGA CTGCTGGGGC AGTCAATGGA TGAGAGTGGA 
TTACCACAGC TGACCAGTTA CGATTGTGAA GTTAATGCTC CCATACAAGG CAGCAGAAAC 
CTACTGCAGG GTGAAGAATT ACTCAGAGCT TTGGATCAAG TTAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006240261.1
CDS	80..2527
Translation	MSSERRKEKSRDAARSRRSKESEVFYELAHQLPLPHNVSSHLDKASVMRLTISYLRVRKLLDAGDLDIEDEMKAQMNCFYLKALDGFVMVLTDDGDMIYISDNVNKYMGLTQFELTGHSVFDFTHPCDHEEMREMLTHRNGPVRKGKEQNTQRSFFLRMKCTLTSRGRTMNIKSATWKVLHCTGHIHVYDTSSNQPQCGYKKPPMTCLVLICEPIPHPSNIEIPLDSKTFLSRHSLDMKFSYCDERITELMGYEPEELLGRSIYEYYHALDSDHLTKTHHDMFTKGQVTTGQYRMLAKRGGYVWVETQATVIYNTKNSQPQCIVCVNYVVSGIIQHDLIFSLQQTESVLKPVESSDMKMTQLFTKVESEDTSCLFDKLKKEPDALTLLAPAAGDTIISLDFGSDDTETEDQQLEDVPLYNDVMFPSSNEKLNINLAMSPLPASETPKPLRSSADPALNQEVALKLESSPESLGLSFTMPQIQDQPASPSDGSTRQSSPEPNSPSEYCFDVDSDMVNVFKLELVEKLFAEDTEAKNPFSAQDTDLDLEMLAPYIPMDDDFQLRSFDQLSPLESNSPSPPSVSTVTGFQQTQLQKPTITVTAATATTATTTDESKAVTKDNIEDIKILIASPPSTQVPQEMTTAKASAYSGTHSRTTSPDRAGKRVIEKTDKAHPRSLNLSVTLNQRNTVPEEELNPKTIALQNAQRKRKMEHDGSLFQAAGIVSGTLLQQPGDRAPTMSLSWKRVKGYISSEQDGMEQKTIFLIPSDLACRLLGQSMDESGLPQLTSYDCEVNAPIQGSRNLLQGEELLRALDQVN

Target ORF information:

RefSeq Version	XM_006240199.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus hypoxia inducible factor 1 alpha subunit (Hif1a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006240199.3

ORF Insert Sequence:

ATGAGTTCCG AACGTCGAAA AGAAAAGTCT AGGGATGCAG CACGATCTCG GCGAAGCAAA 
GAGTCTGAAG TTTTTTATGA GCTTGCTCAT CAGTTGCCAC TTCCCCACAA CGTGAGCTCC 
CATCTTGATA AAGCTTCTGT TATGAGGCTC ACCATCAGTT ACTTACGTGT GAGGAAACTT 
CTAGATGCTG GTGATCTTGA CATTGAAGAT GAAATGAAAG CACAGATGAA CTGCTTTTAT 
CTGAAAGCCC TGGATGGCTT TGTTATGGTG CTAACAGATG ATGGTGACAT GATTTACATT 
TCTGATAACG TGAACAAATA CATGGGGTTG ACTCAGTTTG AACTAACTGG ACACAGTGTG 
TTTGATTTTA CCCATCCATG TGACCATGAG GAAATGAGAG AAATGCTTAC ACACAGAAAT 
GGCCCAGTGA GAAAGGGGAA AGAACAAAAC ACGCAGCGAA GCTTTTTTCT CAGAATGAAA 
TGTACCCTAA CAAGCCGGGG GAGGACGATG AACATCAAGT CAGCAACGTG GAAGGTGCTG 
CACTGCACAG GCCACATTCA TGTGTATGAT ACCAGCAGTA ACCAGCCGCA GTGTGGCTAC 
AAGAAACCGC CTATGACGTG CTTGGTGCTG ATTTGTGAAC CCATTCCTCA TCCATCAAAC 
ATTGAAATTC CTTTAGACAG CAAGACATTT CTCAGTCGAC ACAGCCTCGA TATGAAATTT 
TCTTACTGTG ATGAAAGGAT TACTGAGTTG ATGGGTTATG AGCCAGAAGA ACTTTTGGGC 
CGTTCAATTT ATGAATATTA TCATGCTTTG GACTCTGATC ATCTGACCAA AACTCATCAT 
GACATGTTTA CTAAAGGACA AGTCACCACA GGACAGTACA GGATGCTTGC AAAAAGAGGT 
GGATATGTCT GGGTTGAGAC TCAAGCAACT GTTATATATA ATACGAAGAA CTCTCAGCCA 
CAGTGCATTG TGTGTGTGAA TTATGTTGTA AGTGGTATTA TTCAGCACGA CTTGATTTTC 
TCCCTTCAAC AAACAGAATC TGTCCTCAAA CCAGTTGAAT CTTCAGATAT GAAAATGACC 
CAGCTGTTCA CTAAAGTGGA ATCTGAGGAC ACGAGCTGCC TCTTCGACAA GCTTAAGAAA 
GAGCCCGATG CCCTGACTCT GCTAGCTCCA GCGGCTGGGG ACACGATCAT ATCACTGGAC 
TTCGGCAGCG ATGACACGGA AACTGAAGAC CAACAACTTG AAGATGTCCC GTTGTACAAT 
GATGTAATGT TCCCCTCTTC TAATGAGAAA TTAAATATAA ATCTGGCAAT GTCTCCATTA 
CCTGCCTCTG AAACTCCAAA GCCACTTCGA AGTAGTGCTG ATCCTGCACT GAATCAAGAG 
GTTGCATTGA AGTTAGAGTC AAGCCCAGAG TCACTGGGAC TTTCTTTTAC CATGCCCCAG 
ATTCAAGATC AGCCAGCAAG TCCTTCTGAT GGAAGCACTA GACAAAGCTC ACCTGAGCCT 
AACAGTCCCA GTGAGTACTG CTTTGATGTG GACAGCGATA TGGTCAATGT ATTCAAGTTG 
GAACTGGTGG AAAAACTGTT TGCTGAAGAC ACAGAAGCGA AGAATCCATT TTCAGCTCAG 
GACACTGATT TAGACTTGGA AATGCTGGCT CCCTATATCC CAATGGATGA TGATTTCCAG 
TTACGTTCCT TTGATCAGTT GTCACCATTA GAGAGCAATT CTCCAAGCCC TCCGAGTGTG 
AGCACAGTTA CAGGATTCCA GCAGACCCAG TTACAGAAAC CTACCATCAC TGTCACTGCC 
GCAACTGCCA CCACCGCCAC CACCACTGAT GAATCAAAAG CAGTGACGAA GGACAATATA 
GAAGACATTA AAATACTGAT TGCATCTCCA CCTTCTACCC AAGTACCTCA AGAAATGACC 
ACTGCTAAGG CATCAGCATA CAGTGGTACT CACAGTCGGA CAACCTCACC AGACAGAGCA 
GGAAAGAGAG TCATAGAAAA AACAGACAAA GCTCATCCAA GGAGCCTTAA CCTATCTGTC 
ACTTTGAATC AAAGAAATAC TGTTCCTGAA GAAGAATTAA ACCCAAAGAC AATAGCTTTG 
CAGAATGCTC AGAGGAAGCG AAAAATGGAA CATGATGGCT CCCTTTTTCA AGCAGCAGGA 
ATTGTAAGTG GAACGTTACT GCAGCAACCA GGTGACCGTG CCCCTACTAT GTCGCTTTCT 
TGGAAACGAG TGAAAGGATA CATATCTAGT GAACAGGATG GAATGGAGCA GAAGACAATT 
TTTTTAATAC CCTCTGATTT AGCATGTAGA CTGCTGGGGC AGTCAATGGA TGAGAGTGGA 
TTACCACAGC TGACCAGTTA CGATTGTGAA GTTAATGCTC CCATACAAGG CAGCAGAAAC 
CTACTGCAGG GTGAAGAATT ACTCAGAGCT TTGGATCAAG TTAACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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Hif1a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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