HIF1A cDNA ORF clone, Gallus gallus(chicken)

The following HIF1A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HIF1A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa00359 NM_204297.1
Latest version!
Gallus gallus hypoxia inducible factor 1 subunit alpha (HIF1A), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OGa150728 XM_025150539.1
Latest version!
Gallus gallus hypoxia inducible factor 1 alpha subunit (HIF1A), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OGa36741 XM_015287262.2
Latest version!
Gallus gallus hypoxia inducible factor 1 alpha subunit (HIF1A), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OGa36742 XM_015287264.2
Latest version!
Gallus gallus hypoxia inducible factor 1 alpha subunit (HIF1A), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OGa36742 XM_015287265.2
Latest version!
Gallus gallus hypoxia inducible factor 1 alpha subunit (HIF1A), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OGa36743 XM_015287266.2
Latest version!
Gallus gallus hypoxia inducible factor 1 alpha subunit (HIF1A), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
View Old Accession Versions >>
OGa36742 XM_015287265.1 Gallus gallus hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) (HIF1A), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OGa36742 XM_015287264.1 Gallus gallus hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) (HIF1A), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OGa36743 XM_015287266.1 Gallus gallus hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) (HIF1A), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OGa36741 XM_015287262.1 Gallus gallus hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) (HIF1A), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa00359
    Clone ID Related Accession (Same CDS sequence) NM_204297.1
    Accession Version NM_204297.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2436bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-26
    Organism Gallus gallus(chicken)
    Product hypoxia-inducible factor 1-alpha
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB013746.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB013746.3 [ECO:0000332] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGACAGCC CTGGCGGCGT CACCGACAAG AAGAGGATTA GTTCTGAACG CAGGAAAGAG 
    AAGTCAAGAG ATGCAGCCAG GTGCCGAAGA AGCAAGGAAT CAGAAGTGTT CTATGAACTT
    GCTCATCAGC TGCCCCTTCC GCACACTGTG AGTGCCCACC TTGACAAGGC ATCCATTATG
    AGGCTGACCA TCAGCTACCT GCGCATGAGG AAGCTGCTGG ATGCTGGTGA GCTAGAGACA
    GAAGCCAACA TGGAGAAGGA GCTGAACTGT TTCTACTTAA AAGCTCTTGA TGGATTTGTT
    ATGGTGCTTT CTGAGGATGG GGACATGATT TACATGTCTG AAAACGTGAA CAAGTGTATG
    GGACTCACTC AGTTCGATTT GACGGGGCAC AGTGTATTTG ATTTCACTCA TCCATGTGAC
    CATGAAGAGC TGAGAGAAAT GCTTACACAC AGAAATGGTC CTGTGAAAAA GGGCAAAGAG
    CAAAACACGG AGCGCAGCTT CTTTCTCAGA ATGAAGTGTA CGCTGACCAG CAGGGGAAGA
    ACAGTGAATA TAAAGTCTGC TACATGGAAG GTACTGCACT GCACTGGACA TATACGTGTA
    TATGACACAT GCAATAATCA AACTCACTGT GGCTACAAGA AGCCTCCCAT GACGTGCTTG
    GTGTTGATAT GTGAACCAAT TCCTCACCCA TCAAACATTG AGGTCCCCTT GGACAGTAAA
    ACGTTCCTCA GTCGTCACAG TCTCGATATG AAATTTTCCT ACTGTGATGA AAGAATTACA
    GAGTTGATGG GATATGAACC AGAGGAACTC CTGGGTCGTT CAATCTATGA GTACTATCAT
    GCGCTGGATT CTGATCATCT GACCAAAACA CACCATGATA TGTTCACGAA AGGGCAGGTG
    ACTACGGGAC AATACAGAAT GCTTGCTAAA CAAGGTGGCT ACGTCTGGGT TGAGACTCAA
    GCAACTGTTA TATACAACAC TAAGAATTCT CAACCACAGT GCATAGTATG TGTAAACTAT
    GTGTTGAGTG GAATTGTTCA GAAGGACTTG ATATTTTCCC TTGGACAAAC TGAGTGTATG
    CTGAAACCAG TGGAGTCTCC TGAAATGAAA ATGACCAAAA TATTCAGCAA AGATGACTGG
    GATGATACCA ACAGCCTTTT TGAAAAACTT AAGCAGGAAC CAGATGCTTT AACTGTGCTG
    GCTCCAGCTG CTGGAGACAC AATCATCTCC CTAGATTTCA GCAGCAATGA GTCTGATGAA
    CAACAATGTG ATGAAGTCCC TTTGTATAAC GATGTAATGC TCCCCTCATC CAGTGAGAAA
    TTGCAGAATA TAAATATAGC AATGTCCCCT CTACCTGCCT CTGAAACTAC AAAGCCACTT
    CGTAGCAATG CCGATCCTGC ACTCAATAGA GAAGTTGTAT CAAAGCTGGA GCCAAACACA
    GAGACACTTG AACTTTCTTT TACCATGCCT CAGGTACAAG AGCAACCAAC CAGCCCTTCT
    GATGCAAGTA CCAGCCAAAG TTCACCTGAG CCCAGTAGTC CCAATGACTA TTGCTTTGAT
    GTGGATAATG ACATGGCTAA TGAATTCAAA CTGGAATTAG TGGAGAAGCT CTTTGCAATA
    GATACAGAAG CAAAAAATCC ATTCTCCACT CAGGAGACTG ATTTAGACTT GGAAATGTTG
    GCTCCTTACA TCCCAATGGA TGATGATTTC CAGCTACGCT CCTTTGATCA ACTATCTCCA
    CTGGAAAGCA GTTCTTCTGG CTCTCAAAAT GCAGCCACCA TTACCATACT TCAGCAGACT
    CAGACACCAT CAACTGCTGC TGATGAAATA AAACCAGTGG CAGAACGTGT GGATGATGTG
    AAGGCACTAA TTGTTCCATC ATCTCCTGTC CACGTAATAA ATGACACGAG TAGTGCCCCT
    GCATCACCTT ACAGTGGAAA CAGGAGTCGA ACTGCATCTC CAATCAGAGC AGGAAAAGGA
    ACATTGGAAC AGACAGAAAA ATCTTGTCCA GGAGCACCCA GTTTGATAAC AGTCACGCTG
    AACAAAAGAT CTACTGCAAT GGATGAAGAA CTAAATCCAA AGATGCTAGC TTTGCATAAT
    GCTCAGAGAA AACGAAAAAT GGAACATGAT GGTTCACTTT TTCAGGCAGT TGGAATTGGG
    TCTTTATTCC AGCAGACAGG TGACCGTGGA GGAAACGCAT CGCTTGCTTG GAAACGTGTA
    AAGGCGTGCA AAACAAACGG TCACAATGGA GTGGAGCAAA AGACAATCAT TCTACTATCA
    ACTGACATAG CGAGTAAACT TCTAGGGCAG TCGATGGATG AAAGTGGACT ACCCCAACTC
    ACGAGTTACG ATTGTGAAGT AAATGCTCCC ATACAAGGCA ACAGAAACCT GCTACAGGGT
    GAAGAACTGC TCAGAGCTCT GGATCAAGTT AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_989628.1
    CDS42..2477
    Translation

    Target ORF information:

    RefSeq Version NM_204297.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus hypoxia inducible factor 1 subunit alpha (HIF1A), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_204297.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGACAGCC CTGGCGGCGT CACCGACAAG AAGAGGATTA GTTCTGAACG CAGGAAAGAG 
    AAGTCAAGAG ATGCAGCCAG GTGCCGAAGA AGCAAGGAAT CAGAAGTGTT CTATGAACTT
    GCTCATCAGC TGCCCCTTCC GCACACTGTG AGTGCCCACC TTGACAAGGC ATCCATTATG
    AGGCTGACCA TCAGCTACCT GCGCATGAGG AAGCTGCTGG ATGCTGGTGA GCTAGAGACA
    GAAGCCAACA TGGAGAAGGA GCTGAACTGT TTCTACTTAA AAGCTCTTGA TGGATTTGTT
    ATGGTGCTTT CTGAGGATGG GGACATGATT TACATGTCTG AAAACGTGAA CAAGTGTATG
    GGACTCACTC AGTTCGATTT GACGGGGCAC AGTGTATTTG ATTTCACTCA TCCATGTGAC
    CATGAAGAGC TGAGAGAAAT GCTTACACAC AGAAATGGTC CTGTGAAAAA GGGCAAAGAG
    CAAAACACGG AGCGCAGCTT CTTTCTCAGA ATGAAGTGTA CGCTGACCAG CAGGGGAAGA
    ACAGTGAATA TAAAGTCTGC TACATGGAAG GTACTGCACT GCACTGGACA TATACGTGTA
    TATGACACAT GCAATAATCA AACTCACTGT GGCTACAAGA AGCCTCCCAT GACGTGCTTG
    GTGTTGATAT GTGAACCAAT TCCTCACCCA TCAAACATTG AGGTCCCCTT GGACAGTAAA
    ACGTTCCTCA GTCGTCACAG TCTCGATATG AAATTTTCCT ACTGTGATGA AAGAATTACA
    GAGTTGATGG GATATGAACC AGAGGAACTC CTGGGTCGTT CAATCTATGA GTACTATCAT
    GCGCTGGATT CTGATCATCT GACCAAAACA CACCATGATA TGTTCACGAA AGGGCAGGTG
    ACTACGGGAC AATACAGAAT GCTTGCTAAA CAAGGTGGCT ACGTCTGGGT TGAGACTCAA
    GCAACTGTTA TATACAACAC TAAGAATTCT CAACCACAGT GCATAGTATG TGTAAACTAT
    GTGTTGAGTG GAATTGTTCA GAAGGACTTG ATATTTTCCC TTGGACAAAC TGAGTGTATG
    CTGAAACCAG TGGAGTCTCC TGAAATGAAA ATGACCAAAA TATTCAGCAA AGATGACTGG
    GATGATACCA ACAGCCTTTT TGAAAAACTT AAGCAGGAAC CAGATGCTTT AACTGTGCTG
    GCTCCAGCTG CTGGAGACAC AATCATCTCC CTAGATTTCA GCAGCAATGA GTCTGATGAA
    CAACAATGTG ATGAAGTCCC TTTGTATAAC GATGTAATGC TCCCCTCATC CAGTGAGAAA
    TTGCAGAATA TAAATATAGC AATGTCCCCT CTACCTGCCT CTGAAACTAC AAAGCCACTT
    CGTAGCAATG CCGATCCTGC ACTCAATAGA GAAGTTGTAT CAAAGCTGGA GCCAAACACA
    GAGACACTTG AACTTTCTTT TACCATGCCT CAGGTACAAG AGCAACCAAC CAGCCCTTCT
    GATGCAAGTA CCAGCCAAAG TTCACCTGAG CCCAGTAGTC CCAATGACTA TTGCTTTGAT
    GTGGATAATG ACATGGCTAA TGAATTCAAA CTGGAATTAG TGGAGAAGCT CTTTGCAATA
    GATACAGAAG CAAAAAATCC ATTCTCCACT CAGGAGACTG ATTTAGACTT GGAAATGTTG
    GCTCCTTACA TCCCAATGGA TGATGATTTC CAGCTACGCT CCTTTGATCA ACTATCTCCA
    CTGGAAAGCA GTTCTTCTGG CTCTCAAAAT GCAGCCACCA TTACCATACT TCAGCAGACT
    CAGACACCAT CAACTGCTGC TGATGAAATA AAACCAGTGG CAGAACGTGT GGATGATGTG
    AAGGCACTAA TTGTTCCATC ATCTCCTGTC CACGTAATAA ATGACACGAG TAGTGCCCCT
    GCATCACCTT ACAGTGGAAA CAGGAGTCGA ACTGCATCTC CAATCAGAGC AGGAAAAGGA
    ACATTGGAAC AGACAGAAAA ATCTTGTCCA GGAGCACCCA GTTTGATAAC AGTCACGCTG
    AACAAAAGAT CTACTGCAAT GGATGAAGAA CTAAATCCAA AGATGCTAGC TTTGCATAAT
    GCTCAGAGAA AACGAAAAAT GGAACATGAT GGTTCACTTT TTCAGGCAGT TGGAATTGGG
    TCTTTATTCC AGCAGACAGG TGACCGTGGA GGAAACGCAT CGCTTGCTTG GAAACGTGTA
    AAGGCGTGCA AAACAAACGG TCACAATGGA GTGGAGCAAA AGACAATCAT TCTACTATCA
    ACTGACATAG CGAGTAAACT TCTAGGGCAG TCGATGGATG AAAGTGGACT ACCCCAACTC
    ACGAGTTACG ATTGTGAAGT AAATGCTCCC ATACAAGGCA ACAGAAACCT GCTACAGGGT
    GAAGAACTGC TCAGAGCTCT GGATCAAGTT AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa150728
    Clone ID Related Accession (Same CDS sequence) XM_025150539.1
    Accession Version XM_025150539.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2112bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product hypoxia-inducible factor 1-alpha isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGACAGCC CTGGCGGCGT CACCGACAAG AAGAGGATTA GTTCTGAACG CAGGAAAGAG 
    AAGTCAAGAG ATGCAGCCAG GTGCCGAAGA AGCAAGGAAT CAGAAGTGTT CTATGAACTT
    GCTCATCAGC TGCCCCTTCC GCACACTGTG AGTGCCCACC TTGACAAGGC ATCCATTATG
    AGGCTGACCA TCAGCTACCT GCGCATGAGG AAGCTGCTGG ATGCTGGTGA GCTAGAGACA
    GAAGCCAAAA TGGAGAAGGA GCTGAACTGT TTCTACTTAA AAGCTCTTGA TGGATTTGTT
    ATGGTGCTTT CTGAGGACGG GGACATGATT TACATGTCTG AAAACGTGAA CAAGTGTATG
    GGACTCACTC AGTTCGATTT GACGGGGCAC AGTGTATTTG ATTTCACTCA TCCATGTGAC
    CATGAAGAGC TGAGAGAAAT GCTTACACAC AGAAATGGTC CTGTGAAAAA GGGCAAAGAG
    CAAAACACGG AGCGCAGCTT CTTTCTCAGA ATGAAGTGTA CGCTGACCAG CAGGGGAAGA
    ACAGTGAATA TAAAGTCTGC TACATGGAAG GTACTGCACT GCACTGGACA CATACGTGTA
    TATGACACAT GCAATAATCA AACTCACTGT GGCTACAAGA AGCCTCCCAT GACGTGCTTG
    GTGTTGATAT GTGAACCAAT TCCTCACCCA TCAAACATTG AGGTCCCCTT GGACAGTAAA
    ACGTTCCTCA GTCGTCACAG TCTCGATATG AAATTTTCCT ACTGTGATGA AAGAATTACA
    GAGTTGATGG GATATGAACC AGAGGAACTC CTGGGTCGTT CAATCTATGA GTACTATCAT
    GCGCTGGATT CTGATCATCT GACCAAAACA CACCATGATA TGTTCACGAA AGGGCAGGTG
    ACTACGGGAC AATACAGAAT GCTTGCTAAA CAAGGTGGCT ACGTCTGGGT TGAGACTCAA
    GCAACTGTTA TATACAACAC TAAGAATTCT CAACCACAGT GCATAGTATG TGTAAACTAT
    GTGTTGAGTG GAATTGTTCA GAAGGACTTG ATATTTTCCC TTGGACAAAC TGAGTGTATG
    CTGAAACCAG TGGAGTCTCC TGAAATGAAA ATGACCAAAA TATTCAGCAA AGATGACCTG
    GATGATACCA ACAGCCTTTT TGAAAAACTT AAGCAGGAAC CAGATGCTTT AACTGTGCTG
    GCTCCAGCTG CTGGAGACAC AATCATCTCC CTAGATTTCA GCAGCAATGA GTCTGATGAA
    CAACAATGTG ATGAAGTTCC TTTGTATAAC GATGTAATGC TCCCCTCATC CAGTGAGAAA
    TTGCAGAATA TAAATATGGC AATGTCCCCT CTACCTGCCT CTGAAACTAC AAAGCCACTT
    CGTAGCAATG CCGATCCTGC ACTCAATAGA GAAGTTGTAT CAAAGCTGGA GCCAAACACA
    GAGACACTTG AACTTTCTTT TACCATGCCT CAGGTACAAG AGCAACCAAC CAGCCCTTCT
    GATGCAAGTA CCAGCCAAAG TTCACCTGAG CCCAGTAGTC CCAATGACTA TTGCTTTGAT
    GTGGATAATG ACATGGCTAA TGAATTCAAA CTGGAATTAG TGGAGAAGCT CTTTGCAATA
    GATACAGAAG CAAAAAATCC ATTCTCCACT CAGGAGACTG ATTTAGACTT GGAAATGTTG
    GCTCCTTACA TCCCAATGGA TGATGATTTC CAGCTACGCT CCTTTGATCA ACTATCTCCA
    CTGGAAAGCA GTTCTTCTGG CTCTCAAAAT GCAGCCACCA TTACCATACT TCAGCAGACT
    CAGACACCAT CAACTGCTGC TGATGAAATA AAACCAGTGG CAGAACGTGT GGATGATGTG
    AAGGCACTAA TTGTTCCATC ATCTCCTGTC CACGTAATAA ATGACACGAG TAGTGCCCCT
    GCATCACCTT ACAGTGGAAA CAGGAGTCGA ACTGCATCTC CAATCAGAGC AGGAAAAGGA
    ACATTGGAAC AGACAGAAAA ATCTTGTCCA GGAGCACCCA GTTTGATAAC AGTCACGCTG
    AACAAAAGGG GTCTTTATTC CAGCAGACAG GTGACCGTGG AGGAAACGCA TCGCTTGCTT
    GGAAACGTGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_025006307.1
    CDS216..2327
    Translation

    Target ORF information:

    RefSeq Version XM_025150539.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus hypoxia inducible factor 1 alpha subunit (HIF1A), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025150539.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGACAGCC CTGGCGGCGT CACCGACAAG AAGAGGATTA GTTCTGAACG CAGGAAAGAG 
    AAGTCAAGAG ATGCAGCCAG GTGCCGAAGA AGCAAGGAAT CAGAAGTGTT CTATGAACTT
    GCTCATCAGC TGCCCCTTCC GCACACTGTG AGTGCCCACC TTGACAAGGC ATCCATTATG
    AGGCTGACCA TCAGCTACCT GCGCATGAGG AAGCTGCTGG ATGCTGGTGA GCTAGAGACA
    GAAGCCAAAA TGGAGAAGGA GCTGAACTGT TTCTACTTAA AAGCTCTTGA TGGATTTGTT
    ATGGTGCTTT CTGAGGACGG GGACATGATT TACATGTCTG AAAACGTGAA CAAGTGTATG
    GGACTCACTC AGTTCGATTT GACGGGGCAC AGTGTATTTG ATTTCACTCA TCCATGTGAC
    CATGAAGAGC TGAGAGAAAT GCTTACACAC AGAAATGGTC CTGTGAAAAA GGGCAAAGAG
    CAAAACACGG AGCGCAGCTT CTTTCTCAGA ATGAAGTGTA CGCTGACCAG CAGGGGAAGA
    ACAGTGAATA TAAAGTCTGC TACATGGAAG GTACTGCACT GCACTGGACA CATACGTGTA
    TATGACACAT GCAATAATCA AACTCACTGT GGCTACAAGA AGCCTCCCAT GACGTGCTTG
    GTGTTGATAT GTGAACCAAT TCCTCACCCA TCAAACATTG AGGTCCCCTT GGACAGTAAA
    ACGTTCCTCA GTCGTCACAG TCTCGATATG AAATTTTCCT ACTGTGATGA AAGAATTACA
    GAGTTGATGG GATATGAACC AGAGGAACTC CTGGGTCGTT CAATCTATGA GTACTATCAT
    GCGCTGGATT CTGATCATCT GACCAAAACA CACCATGATA TGTTCACGAA AGGGCAGGTG
    ACTACGGGAC AATACAGAAT GCTTGCTAAA CAAGGTGGCT ACGTCTGGGT TGAGACTCAA
    GCAACTGTTA TATACAACAC TAAGAATTCT CAACCACAGT GCATAGTATG TGTAAACTAT
    GTGTTGAGTG GAATTGTTCA GAAGGACTTG ATATTTTCCC TTGGACAAAC TGAGTGTATG
    CTGAAACCAG TGGAGTCTCC TGAAATGAAA ATGACCAAAA TATTCAGCAA AGATGACCTG
    GATGATACCA ACAGCCTTTT TGAAAAACTT AAGCAGGAAC CAGATGCTTT AACTGTGCTG
    GCTCCAGCTG CTGGAGACAC AATCATCTCC CTAGATTTCA GCAGCAATGA GTCTGATGAA
    CAACAATGTG ATGAAGTTCC TTTGTATAAC GATGTAATGC TCCCCTCATC CAGTGAGAAA
    TTGCAGAATA TAAATATGGC AATGTCCCCT CTACCTGCCT CTGAAACTAC AAAGCCACTT
    CGTAGCAATG CCGATCCTGC ACTCAATAGA GAAGTTGTAT CAAAGCTGGA GCCAAACACA
    GAGACACTTG AACTTTCTTT TACCATGCCT CAGGTACAAG AGCAACCAAC CAGCCCTTCT
    GATGCAAGTA CCAGCCAAAG TTCACCTGAG CCCAGTAGTC CCAATGACTA TTGCTTTGAT
    GTGGATAATG ACATGGCTAA TGAATTCAAA CTGGAATTAG TGGAGAAGCT CTTTGCAATA
    GATACAGAAG CAAAAAATCC ATTCTCCACT CAGGAGACTG ATTTAGACTT GGAAATGTTG
    GCTCCTTACA TCCCAATGGA TGATGATTTC CAGCTACGCT CCTTTGATCA ACTATCTCCA
    CTGGAAAGCA GTTCTTCTGG CTCTCAAAAT GCAGCCACCA TTACCATACT TCAGCAGACT
    CAGACACCAT CAACTGCTGC TGATGAAATA AAACCAGTGG CAGAACGTGT GGATGATGTG
    AAGGCACTAA TTGTTCCATC ATCTCCTGTC CACGTAATAA ATGACACGAG TAGTGCCCCT
    GCATCACCTT ACAGTGGAAA CAGGAGTCGA ACTGCATCTC CAATCAGAGC AGGAAAAGGA
    ACATTGGAAC AGACAGAAAA ATCTTGTCCA GGAGCACCCA GTTTGATAAC AGTCACGCTG
    AACAAAAGGG GTCTTTATTC CAGCAGACAG GTGACCGTGG AGGAAACGCA TCGCTTGCTT
    GGAAACGTGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa36741
    Clone ID Related Accession (Same CDS sequence) XM_015287262.1 , XM_015287262.2
    Accession Version XM_015287262.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2313bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product hypoxia-inducible factor 1-alpha isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455885.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGACAGCC CTGGCGGCGT CACCGACAAG AAGAGGATTA GTTCTGAACG CAGGAAAGAG 
    AAGTCAAGAG ATGCAGCCAG GTGCCGAAGA AGCAAGGAAT CAGAAGTGTT CTATGAACTT
    GCTCATCAGC TGCCCCTTCC GCACACTGTG AGTGCCCACC TTGACAAGGC ATCCATTATG
    AGGCTGACCA TCAGCTACCT GCGCATGAGG AAGCTGCTGG ATGCTGGTGA GCTAGAGACA
    GAAGCCAAAA TGGAGAAGGA GCTGAACTGT TTCTACTTAA AAGCTCTTGA TGGATTTGTT
    ATGGTGCTTT CTGAGGACGG GGACATGATT TACATGTCTG AAAACGTGAA CAAGTGTATG
    GGACTCACTC AGTTCGATTT GACGGGGCAC AGTGTATTTG ATTTCACTCA TCCATGTGAC
    CATGAAGAGC TGAGAGAAAT GCTTACACAC AGAAATGGTC CTGTGAAAAA GGGCAAAGAG
    CAAAACACGG AGCGCAGCTT CTTTCTCAGA ATGAAGTGTA CGCTGACCAG CAGGGGAAGA
    ACAGTGAATA TAAAGTCTGC TACATGGAAG GTACTGCACT GCACTGGACA CATACGTGTA
    TATGACACAT GCAATAATCA AACTCACTGT GGCTACAAGA AGCCTCCCAT GACGTGCTTG
    GTGTTGATAT GTGAACCAAT TCCTCACCCA TCAAACATTG AGGTCCCCTT GGACAGTAAA
    ACGTTCCTCA GTCGTCACAG TCTCGATATG AAATTTTCCT ACTGTGATGA AAGAATTACA
    GAGTTGATGG GATATGAACC AGAGGAACTC CTGGGTCGTT CAATCTATGA GTACTATCAT
    GCGCTGGATT CTGATCATCT GACCAAAACA CACCATGATA TGTTCACGAA AGGGCAGGTG
    ACTACGGGAC AATACAGAAT GCTTGCTAAA CAAGGTGGCT ACGTCTGGGT TGAGACTCAA
    GCAACTGTTA TATACAACAC TAAGAATTCT CAACCACAGT GCATAGTATG TGTAAACTAT
    GTGTTGAGTG GAATTGTTCA GAAGGACTTG ATATTTTCCC TTGGACAAAC TGAGTGTATG
    CTGAAACCAG TGGAGTCTCC TGAAATGAAA ATGACCAAAA TATTCAGCAA AGATGACCTG
    GATGATACCA ACAGCCTTTT TGAAAAACTT AAGCAGGAAC CAGATGCTTT AACTGTGCTG
    GCTCCAGCTG CTGGAGACAC AATCATCTCC CTAGATTTCA GCAGCAATGA GTCTGATGAA
    CAACAATGTG ATGAAGTTCC TTTGTATAAC GATGTAATGC TCCCCTCATC CAGTGAGAAA
    TTGCAGAATA TAAATATGGC AATGTCCCCT CTACCTGCCT CTGAAACTAC AAAGCCACTT
    CGTAGCAATG CCGATCCTGC ACTCAATAGA GAAGTTGTAT CAAAGCTGGA GCCAAACACA
    GAGACACTTG AACTTTCTTT TACCATGCCT CAGGTACAAG AGCAACCAAC CAGCCCTTCT
    GATGCAAGTA CCAGCCAAAG TTCACCTGAG GAGACTGATT TAGACTTGGA AATGTTGGCT
    CCTTACATCC CAATGGATGA TGATTTCCAG CTACGCTCCT TTGATCAACT ATCTCCACTG
    GAAAGCAGTT CTTCTGGCTC TCAAAATGCA GCCACCATTA CCATACTTCA GCAGACTCAG
    ACACCATCAA CTGCTGCTGA TGAAATAAAA CCAGTGGCAG AACGTGTGGA TGATGTGAAG
    GCACTAATTG TTCCATCATC TCCTGTCCAC GTAATAAATG ACACGAGTAG TGCCCCTGCA
    TCACCTTACA GTGGAAACAG GAGTCGAACT GCATCTCCAA TCAGAGCAGG AAAAGGAACA
    TTGGAACAGA CAGAAAAATC TTGTCCAGGA GCACCCAGTT TGATAACAGT CACGCTGAAC
    AAAAGATCTA CTGCAATGGA TGAAGAACTA AATCCAAAGA TGCTAGCTTT GCATAATGCT
    CAGAGAAAAC GAAAAATGGA ACATGATGGT TCACTTTTTC AGGCAGTTGG AATTGGGTCT
    TTATTCCAGC AGACAGGTGA CCGTGGAGGA AACGCATCGC TTGCTTGGAA ACGTGTAAAG
    GCGTGCAAAA CAAACGGTCA CAATGGAGTG GAGCAAAAGA CAATCATTCT ACTATCAACT
    GACATAGCGA GTAAACTTCT AGGGCAGTCG ATGGATGAAA GTGGACTACC CCAACTCACG
    AGTTACGATT GTGAAGTAAA TGCTCCCATA CAAGGCAACA GAAACCTGCT ACAGGGTGAA
    GAACTGCTCA GAGCTCTGGA TCAAGTTAAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015142748.1
    CDS449..2761
    Translation

    Target ORF information:

    RefSeq Version XM_015287262.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) (HIF1A), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015287262.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGACAGCC CTGGCGGCGT CACCGACAAG AAGAGGATTA GTTCTGAACG CAGGAAAGAG 
    AAGTCAAGAG ATGCAGCCAG GTGCCGAAGA AGCAAGGAAT CAGAAGTGTT CTATGAACTT
    GCTCATCAGC TGCCCCTTCC GCACACTGTG AGTGCCCACC TTGACAAGGC ATCCATTATG
    AGGCTGACCA TCAGCTACCT GCGCATGAGG AAGCTGCTGG ATGCTGGTGA GCTAGAGACA
    GAAGCCAAAA TGGAGAAGGA GCTGAACTGT TTCTACTTAA AAGCTCTTGA TGGATTTGTT
    ATGGTGCTTT CTGAGGACGG GGACATGATT TACATGTCTG AAAACGTGAA CAAGTGTATG
    GGACTCACTC AGTTCGATTT GACGGGGCAC AGTGTATTTG ATTTCACTCA TCCATGTGAC
    CATGAAGAGC TGAGAGAAAT GCTTACACAC AGAAATGGTC CTGTGAAAAA GGGCAAAGAG
    CAAAACACGG AGCGCAGCTT CTTTCTCAGA ATGAAGTGTA CGCTGACCAG CAGGGGAAGA
    ACAGTGAATA TAAAGTCTGC TACATGGAAG GTACTGCACT GCACTGGACA CATACGTGTA
    TATGACACAT GCAATAATCA AACTCACTGT GGCTACAAGA AGCCTCCCAT GACGTGCTTG
    GTGTTGATAT GTGAACCAAT TCCTCACCCA TCAAACATTG AGGTCCCCTT GGACAGTAAA
    ACGTTCCTCA GTCGTCACAG TCTCGATATG AAATTTTCCT ACTGTGATGA AAGAATTACA
    GAGTTGATGG GATATGAACC AGAGGAACTC CTGGGTCGTT CAATCTATGA GTACTATCAT
    GCGCTGGATT CTGATCATCT GACCAAAACA CACCATGATA TGTTCACGAA AGGGCAGGTG
    ACTACGGGAC AATACAGAAT GCTTGCTAAA CAAGGTGGCT ACGTCTGGGT TGAGACTCAA
    GCAACTGTTA TATACAACAC TAAGAATTCT CAACCACAGT GCATAGTATG TGTAAACTAT
    GTGTTGAGTG GAATTGTTCA GAAGGACTTG ATATTTTCCC TTGGACAAAC TGAGTGTATG
    CTGAAACCAG TGGAGTCTCC TGAAATGAAA ATGACCAAAA TATTCAGCAA AGATGACCTG
    GATGATACCA ACAGCCTTTT TGAAAAACTT AAGCAGGAAC CAGATGCTTT AACTGTGCTG
    GCTCCAGCTG CTGGAGACAC AATCATCTCC CTAGATTTCA GCAGCAATGA GTCTGATGAA
    CAACAATGTG ATGAAGTTCC TTTGTATAAC GATGTAATGC TCCCCTCATC CAGTGAGAAA
    TTGCAGAATA TAAATATGGC AATGTCCCCT CTACCTGCCT CTGAAACTAC AAAGCCACTT
    CGTAGCAATG CCGATCCTGC ACTCAATAGA GAAGTTGTAT CAAAGCTGGA GCCAAACACA
    GAGACACTTG AACTTTCTTT TACCATGCCT CAGGTACAAG AGCAACCAAC CAGCCCTTCT
    GATGCAAGTA CCAGCCAAAG TTCACCTGAG GAGACTGATT TAGACTTGGA AATGTTGGCT
    CCTTACATCC CAATGGATGA TGATTTCCAG CTACGCTCCT TTGATCAACT ATCTCCACTG
    GAAAGCAGTT CTTCTGGCTC TCAAAATGCA GCCACCATTA CCATACTTCA GCAGACTCAG
    ACACCATCAA CTGCTGCTGA TGAAATAAAA CCAGTGGCAG AACGTGTGGA TGATGTGAAG
    GCACTAATTG TTCCATCATC TCCTGTCCAC GTAATAAATG ACACGAGTAG TGCCCCTGCA
    TCACCTTACA GTGGAAACAG GAGTCGAACT GCATCTCCAA TCAGAGCAGG AAAAGGAACA
    TTGGAACAGA CAGAAAAATC TTGTCCAGGA GCACCCAGTT TGATAACAGT CACGCTGAAC
    AAAAGATCTA CTGCAATGGA TGAAGAACTA AATCCAAAGA TGCTAGCTTT GCATAATGCT
    CAGAGAAAAC GAAAAATGGA ACATGATGGT TCACTTTTTC AGGCAGTTGG AATTGGGTCT
    TTATTCCAGC AGACAGGTGA CCGTGGAGGA AACGCATCGC TTGCTTGGAA ACGTGTAAAG
    GCGTGCAAAA CAAACGGTCA CAATGGAGTG GAGCAAAAGA CAATCATTCT ACTATCAACT
    GACATAGCGA GTAAACTTCT AGGGCAGTCG ATGGATGAAA GTGGACTACC CCAACTCACG
    AGTTACGATT GTGAAGTAAA TGCTCCCATA CAAGGCAACA GAAACCTGCT ACAGGGTGAA
    GAACTGCTCA GAGCTCTGGA TCAAGTTAAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa36741
    Clone ID Related Accession (Same CDS sequence) XM_015287262.1 , XM_015287262.2
    Accession Version XM_015287262.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2313bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product hypoxia-inducible factor 1-alpha isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015287262.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGACAGCC CTGGCGGCGT CACCGACAAG AAGAGGATTA GTTCTGAACG CAGGAAAGAG 
    AAGTCAAGAG ATGCAGCCAG GTGCCGAAGA AGCAAGGAAT CAGAAGTGTT CTATGAACTT
    GCTCATCAGC TGCCCCTTCC GCACACTGTG AGTGCCCACC TTGACAAGGC ATCCATTATG
    AGGCTGACCA TCAGCTACCT GCGCATGAGG AAGCTGCTGG ATGCTGGTGA GCTAGAGACA
    GAAGCCAAAA TGGAGAAGGA GCTGAACTGT TTCTACTTAA AAGCTCTTGA TGGATTTGTT
    ATGGTGCTTT CTGAGGACGG GGACATGATT TACATGTCTG AAAACGTGAA CAAGTGTATG
    GGACTCACTC AGTTCGATTT GACGGGGCAC AGTGTATTTG ATTTCACTCA TCCATGTGAC
    CATGAAGAGC TGAGAGAAAT GCTTACACAC AGAAATGGTC CTGTGAAAAA GGGCAAAGAG
    CAAAACACGG AGCGCAGCTT CTTTCTCAGA ATGAAGTGTA CGCTGACCAG CAGGGGAAGA
    ACAGTGAATA TAAAGTCTGC TACATGGAAG GTACTGCACT GCACTGGACA CATACGTGTA
    TATGACACAT GCAATAATCA AACTCACTGT GGCTACAAGA AGCCTCCCAT GACGTGCTTG
    GTGTTGATAT GTGAACCAAT TCCTCACCCA TCAAACATTG AGGTCCCCTT GGACAGTAAA
    ACGTTCCTCA GTCGTCACAG TCTCGATATG AAATTTTCCT ACTGTGATGA AAGAATTACA
    GAGTTGATGG GATATGAACC AGAGGAACTC CTGGGTCGTT CAATCTATGA GTACTATCAT
    GCGCTGGATT CTGATCATCT GACCAAAACA CACCATGATA TGTTCACGAA AGGGCAGGTG
    ACTACGGGAC AATACAGAAT GCTTGCTAAA CAAGGTGGCT ACGTCTGGGT TGAGACTCAA
    GCAACTGTTA TATACAACAC TAAGAATTCT CAACCACAGT GCATAGTATG TGTAAACTAT
    GTGTTGAGTG GAATTGTTCA GAAGGACTTG ATATTTTCCC TTGGACAAAC TGAGTGTATG
    CTGAAACCAG TGGAGTCTCC TGAAATGAAA ATGACCAAAA TATTCAGCAA AGATGACCTG
    GATGATACCA ACAGCCTTTT TGAAAAACTT AAGCAGGAAC CAGATGCTTT AACTGTGCTG
    GCTCCAGCTG CTGGAGACAC AATCATCTCC CTAGATTTCA GCAGCAATGA GTCTGATGAA
    CAACAATGTG ATGAAGTTCC TTTGTATAAC GATGTAATGC TCCCCTCATC CAGTGAGAAA
    TTGCAGAATA TAAATATGGC AATGTCCCCT CTACCTGCCT CTGAAACTAC AAAGCCACTT
    CGTAGCAATG CCGATCCTGC ACTCAATAGA GAAGTTGTAT CAAAGCTGGA GCCAAACACA
    GAGACACTTG AACTTTCTTT TACCATGCCT CAGGTACAAG AGCAACCAAC CAGCCCTTCT
    GATGCAAGTA CCAGCCAAAG TTCACCTGAG GAGACTGATT TAGACTTGGA AATGTTGGCT
    CCTTACATCC CAATGGATGA TGATTTCCAG CTACGCTCCT TTGATCAACT ATCTCCACTG
    GAAAGCAGTT CTTCTGGCTC TCAAAATGCA GCCACCATTA CCATACTTCA GCAGACTCAG
    ACACCATCAA CTGCTGCTGA TGAAATAAAA CCAGTGGCAG AACGTGTGGA TGATGTGAAG
    GCACTAATTG TTCCATCATC TCCTGTCCAC GTAATAAATG ACACGAGTAG TGCCCCTGCA
    TCACCTTACA GTGGAAACAG GAGTCGAACT GCATCTCCAA TCAGAGCAGG AAAAGGAACA
    TTGGAACAGA CAGAAAAATC TTGTCCAGGA GCACCCAGTT TGATAACAGT CACGCTGAAC
    AAAAGATCTA CTGCAATGGA TGAAGAACTA AATCCAAAGA TGCTAGCTTT GCATAATGCT
    CAGAGAAAAC GAAAAATGGA ACATGATGGT TCACTTTTTC AGGCAGTTGG AATTGGGTCT
    TTATTCCAGC AGACAGGTGA CCGTGGAGGA AACGCATCGC TTGCTTGGAA ACGTGTAAAG
    GCGTGCAAAA CAAACGGTCA CAATGGAGTG GAGCAAAAGA CAATCATTCT ACTATCAACT
    GACATAGCGA GTAAACTTCT AGGGCAGTCG ATGGATGAAA GTGGACTACC CCAACTCACG
    AGTTACGATT GTGAAGTAAA TGCTCCCATA CAAGGCAACA GAAACCTGCT ACAGGGTGAA
    GAACTGCTCA GAGCTCTGGA TCAAGTTAAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015142748.1
    CDS217..2529
    Translation

    Target ORF information:

    RefSeq Version XM_015287262.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus hypoxia inducible factor 1 alpha subunit (HIF1A), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015287262.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGACAGCC CTGGCGGCGT CACCGACAAG AAGAGGATTA GTTCTGAACG CAGGAAAGAG 
    AAGTCAAGAG ATGCAGCCAG GTGCCGAAGA AGCAAGGAAT CAGAAGTGTT CTATGAACTT
    GCTCATCAGC TGCCCCTTCC GCACACTGTG AGTGCCCACC TTGACAAGGC ATCCATTATG
    AGGCTGACCA TCAGCTACCT GCGCATGAGG AAGCTGCTGG ATGCTGGTGA GCTAGAGACA
    GAAGCCAAAA TGGAGAAGGA GCTGAACTGT TTCTACTTAA AAGCTCTTGA TGGATTTGTT
    ATGGTGCTTT CTGAGGACGG GGACATGATT TACATGTCTG AAAACGTGAA CAAGTGTATG
    GGACTCACTC AGTTCGATTT GACGGGGCAC AGTGTATTTG ATTTCACTCA TCCATGTGAC
    CATGAAGAGC TGAGAGAAAT GCTTACACAC AGAAATGGTC CTGTGAAAAA GGGCAAAGAG
    CAAAACACGG AGCGCAGCTT CTTTCTCAGA ATGAAGTGTA CGCTGACCAG CAGGGGAAGA
    ACAGTGAATA TAAAGTCTGC TACATGGAAG GTACTGCACT GCACTGGACA CATACGTGTA
    TATGACACAT GCAATAATCA AACTCACTGT GGCTACAAGA AGCCTCCCAT GACGTGCTTG
    GTGTTGATAT GTGAACCAAT TCCTCACCCA TCAAACATTG AGGTCCCCTT GGACAGTAAA
    ACGTTCCTCA GTCGTCACAG TCTCGATATG AAATTTTCCT ACTGTGATGA AAGAATTACA
    GAGTTGATGG GATATGAACC AGAGGAACTC CTGGGTCGTT CAATCTATGA GTACTATCAT
    GCGCTGGATT CTGATCATCT GACCAAAACA CACCATGATA TGTTCACGAA AGGGCAGGTG
    ACTACGGGAC AATACAGAAT GCTTGCTAAA CAAGGTGGCT ACGTCTGGGT TGAGACTCAA
    GCAACTGTTA TATACAACAC TAAGAATTCT CAACCACAGT GCATAGTATG TGTAAACTAT
    GTGTTGAGTG GAATTGTTCA GAAGGACTTG ATATTTTCCC TTGGACAAAC TGAGTGTATG
    CTGAAACCAG TGGAGTCTCC TGAAATGAAA ATGACCAAAA TATTCAGCAA AGATGACCTG
    GATGATACCA ACAGCCTTTT TGAAAAACTT AAGCAGGAAC CAGATGCTTT AACTGTGCTG
    GCTCCAGCTG CTGGAGACAC AATCATCTCC CTAGATTTCA GCAGCAATGA GTCTGATGAA
    CAACAATGTG ATGAAGTTCC TTTGTATAAC GATGTAATGC TCCCCTCATC CAGTGAGAAA
    TTGCAGAATA TAAATATGGC AATGTCCCCT CTACCTGCCT CTGAAACTAC AAAGCCACTT
    CGTAGCAATG CCGATCCTGC ACTCAATAGA GAAGTTGTAT CAAAGCTGGA GCCAAACACA
    GAGACACTTG AACTTTCTTT TACCATGCCT CAGGTACAAG AGCAACCAAC CAGCCCTTCT
    GATGCAAGTA CCAGCCAAAG TTCACCTGAG GAGACTGATT TAGACTTGGA AATGTTGGCT
    CCTTACATCC CAATGGATGA TGATTTCCAG CTACGCTCCT TTGATCAACT ATCTCCACTG
    GAAAGCAGTT CTTCTGGCTC TCAAAATGCA GCCACCATTA CCATACTTCA GCAGACTCAG
    ACACCATCAA CTGCTGCTGA TGAAATAAAA CCAGTGGCAG AACGTGTGGA TGATGTGAAG
    GCACTAATTG TTCCATCATC TCCTGTCCAC GTAATAAATG ACACGAGTAG TGCCCCTGCA
    TCACCTTACA GTGGAAACAG GAGTCGAACT GCATCTCCAA TCAGAGCAGG AAAAGGAACA
    TTGGAACAGA CAGAAAAATC TTGTCCAGGA GCACCCAGTT TGATAACAGT CACGCTGAAC
    AAAAGATCTA CTGCAATGGA TGAAGAACTA AATCCAAAGA TGCTAGCTTT GCATAATGCT
    CAGAGAAAAC GAAAAATGGA ACATGATGGT TCACTTTTTC AGGCAGTTGG AATTGGGTCT
    TTATTCCAGC AGACAGGTGA CCGTGGAGGA AACGCATCGC TTGCTTGGAA ACGTGTAAAG
    GCGTGCAAAA CAAACGGTCA CAATGGAGTG GAGCAAAAGA CAATCATTCT ACTATCAACT
    GACATAGCGA GTAAACTTCT AGGGCAGTCG ATGGATGAAA GTGGACTACC CCAACTCACG
    AGTTACGATT GTGAAGTAAA TGCTCCCATA CAAGGCAACA GAAACCTGCT ACAGGGTGAA
    GAACTGCTCA GAGCTCTGGA TCAAGTTAAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa36742
    Clone ID Related Accession (Same CDS sequence) XM_015287264.1 , XM_015287265.2 , XM_015287265.1 , XM_015287264.2
    Accession Version XM_015287264.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2403bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product hypoxia-inducible factor 1-alpha isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455885.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGATTAGTT CTGAACGCAG GAAAGAGAAG TCAAGAGATG CAGCCAGGTG CCGAAGAAGC 
    AAGGAATCAG AAGTGTTCTA TGAACTTGCT CATCAGCTGC CCCTTCCGCA CACTGTGAGT
    GCCCACCTTG ACAAGGCATC CATTATGAGG CTGACCATCA GCTACCTGCG CATGAGGAAG
    CTGCTGGATG CTGGTGAGCT AGAGACAGAA GCCAAAATGG AGAAGGAGCT GAACTGTTTC
    TACTTAAAAG CTCTTGATGG ATTTGTTATG GTGCTTTCTG AGGACGGGGA CATGATTTAC
    ATGTCTGAAA ACGTGAACAA GTGTATGGGA CTCACTCAGT TCGATTTGAC GGGGCACAGT
    GTATTTGATT TCACTCATCC ATGTGACCAT GAAGAGCTGA GAGAAATGCT TACACACAGA
    AATGGTCCTG TGAAAAAGGG CAAAGAGCAA AACACGGAGC GCAGCTTCTT TCTCAGAATG
    AAGTGTACGC TGACCAGCAG GGGAAGAACA GTGAATATAA AGTCTGCTAC ATGGAAGGTA
    CTGCACTGCA CTGGACACAT ACGTGTATAT GACACATGCA ATAATCAAAC TCACTGTGGC
    TACAAGAAGC CTCCCATGAC GTGCTTGGTG TTGATATGTG AACCAATTCC TCACCCATCA
    AACATTGAGG TCCCCTTGGA CAGTAAAACG TTCCTCAGTC GTCACAGTCT CGATATGAAA
    TTTTCCTACT GTGATGAAAG AATTACAGAG TTGATGGGAT ATGAACCAGA GGAACTCCTG
    GGTCGTTCAA TCTATGAGTA CTATCATGCG CTGGATTCTG ATCATCTGAC CAAAACACAC
    CATGATATGT TCACGAAAGG GCAGGTGACT ACGGGACAAT ACAGAATGCT TGCTAAACAA
    GGTGGCTACG TCTGGGTTGA GACTCAAGCA ACTGTTATAT ACAACACTAA GAATTCTCAA
    CCACAGTGCA TAGTATGTGT AAACTATGTG TTGAGTGGAA TTGTTCAGAA GGACTTGATA
    TTTTCCCTTG GACAAACTGA GTGTATGCTG AAACCAGTGG AGTCTCCTGA AATGAAAATG
    ACCAAAATAT TCAGCAAAGA TGACCTGGAT GATACCAACA GCCTTTTTGA AAAACTTAAG
    CAGGAACCAG ATGCTTTAAC TGTGCTGGCT CCAGCTGCTG GAGACACAAT CATCTCCCTA
    GATTTCAGCA GCAATGAGTC TGATGAACAA CAATGTGATG AAGTTCCTTT GTATAACGAT
    GTAATGCTCC CCTCATCCAG TGAGAAATTG CAGAATATAA ATATGGCAAT GTCCCCTCTA
    CCTGCCTCTG AAACTACAAA GCCACTTCGT AGCAATGCCG ATCCTGCACT CAATAGAGAA
    GTTGTATCAA AGCTGGAGCC AAACACAGAG ACACTTGAAC TTTCTTTTAC CATGCCTCAG
    GTACAAGAGC AACCAACCAG CCCTTCTGAT GCAAGTACCA GCCAAAGTTC ACCTGAGCCC
    AGTAGTCCCA ATGACTATTG CTTTGATGTG GATAATGACA TGGCTAATGA ATTCAAACTG
    GAATTAGTGG AGAAGCTCTT TGCAATAGAT ACAGAAGCAA AAAATCCATT CTCCACTCAG
    GAGACTGATT TAGACTTGGA AATGTTGGCT CCTTACATCC CAATGGATGA TGATTTCCAG
    CTACGCTCCT TTGATCAACT ATCTCCACTG GAAAGCAGTT CTTCTGGCTC TCAAAATGCA
    GCCACCATTA CCATACTTCA GCAGACTCAG ACACCATCAA CTGCTGCTGA TGAAATAAAA
    CCAGTGGCAG AACGTGTGGA TGATGTGAAG GCACTAATTG TTCCATCATC TCCTGTCCAC
    GTAATAAATG ACACGAGTAG TGCCCCTGCA TCACCTTACA GTGGAAACAG GAGTCGAACT
    GCATCTCCAA TCAGAGCAGG AAAAGGAACA TTGGAACAGA CAGAAAAATC TTGTCCAGGA
    GCACCCAGTT TGATAACAGT CACGCTGAAC AAAAGATCTA CTGCAATGGA TGAAGAACTA
    AATCCAAAGA TGCTAGCTTT GCATAATGCT CAGAGAAAAC GAAAAATGGA ACATGATGGT
    TCACTTTTTC AGGCAGTTGG AATTGGGTCT TTATTCCAGC AGACAGGTGA CCGTGGAGGA
    AACGCATCGC TTGCTTGGAA ACGTGTAAAG GCGTGCAAAA CAAACGGTCA CAATGGAGTG
    GAGCAAAAGA CAATCATTCT ACTATCAACT GACATAGCGA GTAAACTTCT AGGGCAGTCG
    ATGGATGAAA GTGGACTACC CCAACTCACG AGTTACGATT GTGAAGTAAA TGCTCCCATA
    CAAGGCAACA GAAACCTGCT ACAGGGTGAA GAACTGCTCA GAGCTCTGGA TCAAGTTAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015142750.1
    CDS197..2599
    Translation

    Target ORF information:

    RefSeq Version XM_015287264.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) (HIF1A), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015287264.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGATTAGTT CTGAACGCAG GAAAGAGAAG TCAAGAGATG CAGCCAGGTG CCGAAGAAGC 
    AAGGAATCAG AAGTGTTCTA TGAACTTGCT CATCAGCTGC CCCTTCCGCA CACTGTGAGT
    GCCCACCTTG ACAAGGCATC CATTATGAGG CTGACCATCA GCTACCTGCG CATGAGGAAG
    CTGCTGGATG CTGGTGAGCT AGAGACAGAA GCCAAAATGG AGAAGGAGCT GAACTGTTTC
    TACTTAAAAG CTCTTGATGG ATTTGTTATG GTGCTTTCTG AGGACGGGGA CATGATTTAC
    ATGTCTGAAA ACGTGAACAA GTGTATGGGA CTCACTCAGT TCGATTTGAC GGGGCACAGT
    GTATTTGATT TCACTCATCC ATGTGACCAT GAAGAGCTGA GAGAAATGCT TACACACAGA
    AATGGTCCTG TGAAAAAGGG CAAAGAGCAA AACACGGAGC GCAGCTTCTT TCTCAGAATG
    AAGTGTACGC TGACCAGCAG GGGAAGAACA GTGAATATAA AGTCTGCTAC ATGGAAGGTA
    CTGCACTGCA CTGGACACAT ACGTGTATAT GACACATGCA ATAATCAAAC TCACTGTGGC
    TACAAGAAGC CTCCCATGAC GTGCTTGGTG TTGATATGTG AACCAATTCC TCACCCATCA
    AACATTGAGG TCCCCTTGGA CAGTAAAACG TTCCTCAGTC GTCACAGTCT CGATATGAAA
    TTTTCCTACT GTGATGAAAG AATTACAGAG TTGATGGGAT ATGAACCAGA GGAACTCCTG
    GGTCGTTCAA TCTATGAGTA CTATCATGCG CTGGATTCTG ATCATCTGAC CAAAACACAC
    CATGATATGT TCACGAAAGG GCAGGTGACT ACGGGACAAT ACAGAATGCT TGCTAAACAA
    GGTGGCTACG TCTGGGTTGA GACTCAAGCA ACTGTTATAT ACAACACTAA GAATTCTCAA
    CCACAGTGCA TAGTATGTGT AAACTATGTG TTGAGTGGAA TTGTTCAGAA GGACTTGATA
    TTTTCCCTTG GACAAACTGA GTGTATGCTG AAACCAGTGG AGTCTCCTGA AATGAAAATG
    ACCAAAATAT TCAGCAAAGA TGACCTGGAT GATACCAACA GCCTTTTTGA AAAACTTAAG
    CAGGAACCAG ATGCTTTAAC TGTGCTGGCT CCAGCTGCTG GAGACACAAT CATCTCCCTA
    GATTTCAGCA GCAATGAGTC TGATGAACAA CAATGTGATG AAGTTCCTTT GTATAACGAT
    GTAATGCTCC CCTCATCCAG TGAGAAATTG CAGAATATAA ATATGGCAAT GTCCCCTCTA
    CCTGCCTCTG AAACTACAAA GCCACTTCGT AGCAATGCCG ATCCTGCACT CAATAGAGAA
    GTTGTATCAA AGCTGGAGCC AAACACAGAG ACACTTGAAC TTTCTTTTAC CATGCCTCAG
    GTACAAGAGC AACCAACCAG CCCTTCTGAT GCAAGTACCA GCCAAAGTTC ACCTGAGCCC
    AGTAGTCCCA ATGACTATTG CTTTGATGTG GATAATGACA TGGCTAATGA ATTCAAACTG
    GAATTAGTGG AGAAGCTCTT TGCAATAGAT ACAGAAGCAA AAAATCCATT CTCCACTCAG
    GAGACTGATT TAGACTTGGA AATGTTGGCT CCTTACATCC CAATGGATGA TGATTTCCAG
    CTACGCTCCT TTGATCAACT ATCTCCACTG GAAAGCAGTT CTTCTGGCTC TCAAAATGCA
    GCCACCATTA CCATACTTCA GCAGACTCAG ACACCATCAA CTGCTGCTGA TGAAATAAAA
    CCAGTGGCAG AACGTGTGGA TGATGTGAAG GCACTAATTG TTCCATCATC TCCTGTCCAC
    GTAATAAATG ACACGAGTAG TGCCCCTGCA TCACCTTACA GTGGAAACAG GAGTCGAACT
    GCATCTCCAA TCAGAGCAGG AAAAGGAACA TTGGAACAGA CAGAAAAATC TTGTCCAGGA
    GCACCCAGTT TGATAACAGT CACGCTGAAC AAAAGATCTA CTGCAATGGA TGAAGAACTA
    AATCCAAAGA TGCTAGCTTT GCATAATGCT CAGAGAAAAC GAAAAATGGA ACATGATGGT
    TCACTTTTTC AGGCAGTTGG AATTGGGTCT TTATTCCAGC AGACAGGTGA CCGTGGAGGA
    AACGCATCGC TTGCTTGGAA ACGTGTAAAG GCGTGCAAAA CAAACGGTCA CAATGGAGTG
    GAGCAAAAGA CAATCATTCT ACTATCAACT GACATAGCGA GTAAACTTCT AGGGCAGTCG
    ATGGATGAAA GTGGACTACC CCAACTCACG AGTTACGATT GTGAAGTAAA TGCTCCCATA
    CAAGGCAACA GAAACCTGCT ACAGGGTGAA GAACTGCTCA GAGCTCTGGA TCAAGTTAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa36742
    Clone ID Related Accession (Same CDS sequence) XM_015287264.1 , XM_015287265.2 , XM_015287265.1 , XM_015287264.2
    Accession Version XM_015287265.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2403bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product hypoxia-inducible factor 1-alpha isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015287265.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGATTAGTT CTGAACGCAG GAAAGAGAAG TCAAGAGATG CAGCCAGGTG CCGAAGAAGC 
    AAGGAATCAG AAGTGTTCTA TGAACTTGCT CATCAGCTGC CCCTTCCGCA CACTGTGAGT
    GCCCACCTTG ACAAGGCATC CATTATGAGG CTGACCATCA GCTACCTGCG CATGAGGAAG
    CTGCTGGATG CTGGTGAGCT AGAGACAGAA GCCAAAATGG AGAAGGAGCT GAACTGTTTC
    TACTTAAAAG CTCTTGATGG ATTTGTTATG GTGCTTTCTG AGGACGGGGA CATGATTTAC
    ATGTCTGAAA ACGTGAACAA GTGTATGGGA CTCACTCAGT TCGATTTGAC GGGGCACAGT
    GTATTTGATT TCACTCATCC ATGTGACCAT GAAGAGCTGA GAGAAATGCT TACACACAGA
    AATGGTCCTG TGAAAAAGGG CAAAGAGCAA AACACGGAGC GCAGCTTCTT TCTCAGAATG
    AAGTGTACGC TGACCAGCAG GGGAAGAACA GTGAATATAA AGTCTGCTAC ATGGAAGGTA
    CTGCACTGCA CTGGACACAT ACGTGTATAT GACACATGCA ATAATCAAAC TCACTGTGGC
    TACAAGAAGC CTCCCATGAC GTGCTTGGTG TTGATATGTG AACCAATTCC TCACCCATCA
    AACATTGAGG TCCCCTTGGA CAGTAAAACG TTCCTCAGTC GTCACAGTCT CGATATGAAA
    TTTTCCTACT GTGATGAAAG AATTACAGAG TTGATGGGAT ATGAACCAGA GGAACTCCTG
    GGTCGTTCAA TCTATGAGTA CTATCATGCG CTGGATTCTG ATCATCTGAC CAAAACACAC
    CATGATATGT TCACGAAAGG GCAGGTGACT ACGGGACAAT ACAGAATGCT TGCTAAACAA
    GGTGGCTACG TCTGGGTTGA GACTCAAGCA ACTGTTATAT ACAACACTAA GAATTCTCAA
    CCACAGTGCA TAGTATGTGT AAACTATGTG TTGAGTGGAA TTGTTCAGAA GGACTTGATA
    TTTTCCCTTG GACAAACTGA GTGTATGCTG AAACCAGTGG AGTCTCCTGA AATGAAAATG
    ACCAAAATAT TCAGCAAAGA TGACCTGGAT GATACCAACA GCCTTTTTGA AAAACTTAAG
    CAGGAACCAG ATGCTTTAAC TGTGCTGGCT CCAGCTGCTG GAGACACAAT CATCTCCCTA
    GATTTCAGCA GCAATGAGTC TGATGAACAA CAATGTGATG AAGTTCCTTT GTATAACGAT
    GTAATGCTCC CCTCATCCAG TGAGAAATTG CAGAATATAA ATATGGCAAT GTCCCCTCTA
    CCTGCCTCTG AAACTACAAA GCCACTTCGT AGCAATGCCG ATCCTGCACT CAATAGAGAA
    GTTGTATCAA AGCTGGAGCC AAACACAGAG ACACTTGAAC TTTCTTTTAC CATGCCTCAG
    GTACAAGAGC AACCAACCAG CCCTTCTGAT GCAAGTACCA GCCAAAGTTC ACCTGAGCCC
    AGTAGTCCCA ATGACTATTG CTTTGATGTG GATAATGACA TGGCTAATGA ATTCAAACTG
    GAATTAGTGG AGAAGCTCTT TGCAATAGAT ACAGAAGCAA AAAATCCATT CTCCACTCAG
    GAGACTGATT TAGACTTGGA AATGTTGGCT CCTTACATCC CAATGGATGA TGATTTCCAG
    CTACGCTCCT TTGATCAACT ATCTCCACTG GAAAGCAGTT CTTCTGGCTC TCAAAATGCA
    GCCACCATTA CCATACTTCA GCAGACTCAG ACACCATCAA CTGCTGCTGA TGAAATAAAA
    CCAGTGGCAG AACGTGTGGA TGATGTGAAG GCACTAATTG TTCCATCATC TCCTGTCCAC
    GTAATAAATG ACACGAGTAG TGCCCCTGCA TCACCTTACA GTGGAAACAG GAGTCGAACT
    GCATCTCCAA TCAGAGCAGG AAAAGGAACA TTGGAACAGA CAGAAAAATC TTGTCCAGGA
    GCACCCAGTT TGATAACAGT CACGCTGAAC AAAAGATCTA CTGCAATGGA TGAAGAACTA
    AATCCAAAGA TGCTAGCTTT GCATAATGCT CAGAGAAAAC GAAAAATGGA ACATGATGGT
    TCACTTTTTC AGGCAGTTGG AATTGGGTCT TTATTCCAGC AGACAGGTGA CCGTGGAGGA
    AACGCATCGC TTGCTTGGAA ACGTGTAAAG GCGTGCAAAA CAAACGGTCA CAATGGAGTG
    GAGCAAAAGA CAATCATTCT ACTATCAACT GACATAGCGA GTAAACTTCT AGGGCAGTCG
    ATGGATGAAA GTGGACTACC CCAACTCACG AGTTACGATT GTGAAGTAAA TGCTCCCATA
    CAAGGCAACA GAAACCTGCT ACAGGGTGAA GAACTGCTCA GAGCTCTGGA TCAAGTTAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015142751.1
    CDS2618..5020
    Translation

    Target ORF information:

    RefSeq Version XM_015287265.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus hypoxia inducible factor 1 alpha subunit (HIF1A), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015287265.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGATTAGTT CTGAACGCAG GAAAGAGAAG TCAAGAGATG CAGCCAGGTG CCGAAGAAGC 
    AAGGAATCAG AAGTGTTCTA TGAACTTGCT CATCAGCTGC CCCTTCCGCA CACTGTGAGT
    GCCCACCTTG ACAAGGCATC CATTATGAGG CTGACCATCA GCTACCTGCG CATGAGGAAG
    CTGCTGGATG CTGGTGAGCT AGAGACAGAA GCCAAAATGG AGAAGGAGCT GAACTGTTTC
    TACTTAAAAG CTCTTGATGG ATTTGTTATG GTGCTTTCTG AGGACGGGGA CATGATTTAC
    ATGTCTGAAA ACGTGAACAA GTGTATGGGA CTCACTCAGT TCGATTTGAC GGGGCACAGT
    GTATTTGATT TCACTCATCC ATGTGACCAT GAAGAGCTGA GAGAAATGCT TACACACAGA
    AATGGTCCTG TGAAAAAGGG CAAAGAGCAA AACACGGAGC GCAGCTTCTT TCTCAGAATG
    AAGTGTACGC TGACCAGCAG GGGAAGAACA GTGAATATAA AGTCTGCTAC ATGGAAGGTA
    CTGCACTGCA CTGGACACAT ACGTGTATAT GACACATGCA ATAATCAAAC TCACTGTGGC
    TACAAGAAGC CTCCCATGAC GTGCTTGGTG TTGATATGTG AACCAATTCC TCACCCATCA
    AACATTGAGG TCCCCTTGGA CAGTAAAACG TTCCTCAGTC GTCACAGTCT CGATATGAAA
    TTTTCCTACT GTGATGAAAG AATTACAGAG TTGATGGGAT ATGAACCAGA GGAACTCCTG
    GGTCGTTCAA TCTATGAGTA CTATCATGCG CTGGATTCTG ATCATCTGAC CAAAACACAC
    CATGATATGT TCACGAAAGG GCAGGTGACT ACGGGACAAT ACAGAATGCT TGCTAAACAA
    GGTGGCTACG TCTGGGTTGA GACTCAAGCA ACTGTTATAT ACAACACTAA GAATTCTCAA
    CCACAGTGCA TAGTATGTGT AAACTATGTG TTGAGTGGAA TTGTTCAGAA GGACTTGATA
    TTTTCCCTTG GACAAACTGA GTGTATGCTG AAACCAGTGG AGTCTCCTGA AATGAAAATG
    ACCAAAATAT TCAGCAAAGA TGACCTGGAT GATACCAACA GCCTTTTTGA AAAACTTAAG
    CAGGAACCAG ATGCTTTAAC TGTGCTGGCT CCAGCTGCTG GAGACACAAT CATCTCCCTA
    GATTTCAGCA GCAATGAGTC TGATGAACAA CAATGTGATG AAGTTCCTTT GTATAACGAT
    GTAATGCTCC CCTCATCCAG TGAGAAATTG CAGAATATAA ATATGGCAAT GTCCCCTCTA
    CCTGCCTCTG AAACTACAAA GCCACTTCGT AGCAATGCCG ATCCTGCACT CAATAGAGAA
    GTTGTATCAA AGCTGGAGCC AAACACAGAG ACACTTGAAC TTTCTTTTAC CATGCCTCAG
    GTACAAGAGC AACCAACCAG CCCTTCTGAT GCAAGTACCA GCCAAAGTTC ACCTGAGCCC
    AGTAGTCCCA ATGACTATTG CTTTGATGTG GATAATGACA TGGCTAATGA ATTCAAACTG
    GAATTAGTGG AGAAGCTCTT TGCAATAGAT ACAGAAGCAA AAAATCCATT CTCCACTCAG
    GAGACTGATT TAGACTTGGA AATGTTGGCT CCTTACATCC CAATGGATGA TGATTTCCAG
    CTACGCTCCT TTGATCAACT ATCTCCACTG GAAAGCAGTT CTTCTGGCTC TCAAAATGCA
    GCCACCATTA CCATACTTCA GCAGACTCAG ACACCATCAA CTGCTGCTGA TGAAATAAAA
    CCAGTGGCAG AACGTGTGGA TGATGTGAAG GCACTAATTG TTCCATCATC TCCTGTCCAC
    GTAATAAATG ACACGAGTAG TGCCCCTGCA TCACCTTACA GTGGAAACAG GAGTCGAACT
    GCATCTCCAA TCAGAGCAGG AAAAGGAACA TTGGAACAGA CAGAAAAATC TTGTCCAGGA
    GCACCCAGTT TGATAACAGT CACGCTGAAC AAAAGATCTA CTGCAATGGA TGAAGAACTA
    AATCCAAAGA TGCTAGCTTT GCATAATGCT CAGAGAAAAC GAAAAATGGA ACATGATGGT
    TCACTTTTTC AGGCAGTTGG AATTGGGTCT TTATTCCAGC AGACAGGTGA CCGTGGAGGA
    AACGCATCGC TTGCTTGGAA ACGTGTAAAG GCGTGCAAAA CAAACGGTCA CAATGGAGTG
    GAGCAAAAGA CAATCATTCT ACTATCAACT GACATAGCGA GTAAACTTCT AGGGCAGTCG
    ATGGATGAAA GTGGACTACC CCAACTCACG AGTTACGATT GTGAAGTAAA TGCTCCCATA
    CAAGGCAACA GAAACCTGCT ACAGGGTGAA GAACTGCTCA GAGCTCTGGA TCAAGTTAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa36742
    Clone ID Related Accession (Same CDS sequence) XM_015287264.1 , XM_015287265.2 , XM_015287265.1 , XM_015287264.2
    Accession Version XM_015287265.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2403bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product hypoxia-inducible factor 1-alpha isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455885.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGATTAGTT CTGAACGCAG GAAAGAGAAG TCAAGAGATG CAGCCAGGTG CCGAAGAAGC 
    AAGGAATCAG AAGTGTTCTA TGAACTTGCT CATCAGCTGC CCCTTCCGCA CACTGTGAGT
    GCCCACCTTG ACAAGGCATC CATTATGAGG CTGACCATCA GCTACCTGCG CATGAGGAAG
    CTGCTGGATG CTGGTGAGCT AGAGACAGAA GCCAAAATGG AGAAGGAGCT GAACTGTTTC
    TACTTAAAAG CTCTTGATGG ATTTGTTATG GTGCTTTCTG AGGACGGGGA CATGATTTAC
    ATGTCTGAAA ACGTGAACAA GTGTATGGGA CTCACTCAGT TCGATTTGAC GGGGCACAGT
    GTATTTGATT TCACTCATCC ATGTGACCAT GAAGAGCTGA GAGAAATGCT TACACACAGA
    AATGGTCCTG TGAAAAAGGG CAAAGAGCAA AACACGGAGC GCAGCTTCTT TCTCAGAATG
    AAGTGTACGC TGACCAGCAG GGGAAGAACA GTGAATATAA AGTCTGCTAC ATGGAAGGTA
    CTGCACTGCA CTGGACACAT ACGTGTATAT GACACATGCA ATAATCAAAC TCACTGTGGC
    TACAAGAAGC CTCCCATGAC GTGCTTGGTG TTGATATGTG AACCAATTCC TCACCCATCA
    AACATTGAGG TCCCCTTGGA CAGTAAAACG TTCCTCAGTC GTCACAGTCT CGATATGAAA
    TTTTCCTACT GTGATGAAAG AATTACAGAG TTGATGGGAT ATGAACCAGA GGAACTCCTG
    GGTCGTTCAA TCTATGAGTA CTATCATGCG CTGGATTCTG ATCATCTGAC CAAAACACAC
    CATGATATGT TCACGAAAGG GCAGGTGACT ACGGGACAAT ACAGAATGCT TGCTAAACAA
    GGTGGCTACG TCTGGGTTGA GACTCAAGCA ACTGTTATAT ACAACACTAA GAATTCTCAA
    CCACAGTGCA TAGTATGTGT AAACTATGTG TTGAGTGGAA TTGTTCAGAA GGACTTGATA
    TTTTCCCTTG GACAAACTGA GTGTATGCTG AAACCAGTGG AGTCTCCTGA AATGAAAATG
    ACCAAAATAT TCAGCAAAGA TGACCTGGAT GATACCAACA GCCTTTTTGA AAAACTTAAG
    CAGGAACCAG ATGCTTTAAC TGTGCTGGCT CCAGCTGCTG GAGACACAAT CATCTCCCTA
    GATTTCAGCA GCAATGAGTC TGATGAACAA CAATGTGATG AAGTTCCTTT GTATAACGAT
    GTAATGCTCC CCTCATCCAG TGAGAAATTG CAGAATATAA ATATGGCAAT GTCCCCTCTA
    CCTGCCTCTG AAACTACAAA GCCACTTCGT AGCAATGCCG ATCCTGCACT CAATAGAGAA
    GTTGTATCAA AGCTGGAGCC AAACACAGAG ACACTTGAAC TTTCTTTTAC CATGCCTCAG
    GTACAAGAGC AACCAACCAG CCCTTCTGAT GCAAGTACCA GCCAAAGTTC ACCTGAGCCC
    AGTAGTCCCA ATGACTATTG CTTTGATGTG GATAATGACA TGGCTAATGA ATTCAAACTG
    GAATTAGTGG AGAAGCTCTT TGCAATAGAT ACAGAAGCAA AAAATCCATT CTCCACTCAG
    GAGACTGATT TAGACTTGGA AATGTTGGCT CCTTACATCC CAATGGATGA TGATTTCCAG
    CTACGCTCCT TTGATCAACT ATCTCCACTG GAAAGCAGTT CTTCTGGCTC TCAAAATGCA
    GCCACCATTA CCATACTTCA GCAGACTCAG ACACCATCAA CTGCTGCTGA TGAAATAAAA
    CCAGTGGCAG AACGTGTGGA TGATGTGAAG GCACTAATTG TTCCATCATC TCCTGTCCAC
    GTAATAAATG ACACGAGTAG TGCCCCTGCA TCACCTTACA GTGGAAACAG GAGTCGAACT
    GCATCTCCAA TCAGAGCAGG AAAAGGAACA TTGGAACAGA CAGAAAAATC TTGTCCAGGA
    GCACCCAGTT TGATAACAGT CACGCTGAAC AAAAGATCTA CTGCAATGGA TGAAGAACTA
    AATCCAAAGA TGCTAGCTTT GCATAATGCT CAGAGAAAAC GAAAAATGGA ACATGATGGT
    TCACTTTTTC AGGCAGTTGG AATTGGGTCT TTATTCCAGC AGACAGGTGA CCGTGGAGGA
    AACGCATCGC TTGCTTGGAA ACGTGTAAAG GCGTGCAAAA CAAACGGTCA CAATGGAGTG
    GAGCAAAAGA CAATCATTCT ACTATCAACT GACATAGCGA GTAAACTTCT AGGGCAGTCG
    ATGGATGAAA GTGGACTACC CCAACTCACG AGTTACGATT GTGAAGTAAA TGCTCCCATA
    CAAGGCAACA GAAACCTGCT ACAGGGTGAA GAACTGCTCA GAGCTCTGGA TCAAGTTAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015142751.1
    CDS1010..3412
    Translation

    Target ORF information:

    RefSeq Version XM_015287265.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) (HIF1A), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015287265.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGATTAGTT CTGAACGCAG GAAAGAGAAG TCAAGAGATG CAGCCAGGTG CCGAAGAAGC 
    AAGGAATCAG AAGTGTTCTA TGAACTTGCT CATCAGCTGC CCCTTCCGCA CACTGTGAGT
    GCCCACCTTG ACAAGGCATC CATTATGAGG CTGACCATCA GCTACCTGCG CATGAGGAAG
    CTGCTGGATG CTGGTGAGCT AGAGACAGAA GCCAAAATGG AGAAGGAGCT GAACTGTTTC
    TACTTAAAAG CTCTTGATGG ATTTGTTATG GTGCTTTCTG AGGACGGGGA CATGATTTAC
    ATGTCTGAAA ACGTGAACAA GTGTATGGGA CTCACTCAGT TCGATTTGAC GGGGCACAGT
    GTATTTGATT TCACTCATCC ATGTGACCAT GAAGAGCTGA GAGAAATGCT TACACACAGA
    AATGGTCCTG TGAAAAAGGG CAAAGAGCAA AACACGGAGC GCAGCTTCTT TCTCAGAATG
    AAGTGTACGC TGACCAGCAG GGGAAGAACA GTGAATATAA AGTCTGCTAC ATGGAAGGTA
    CTGCACTGCA CTGGACACAT ACGTGTATAT GACACATGCA ATAATCAAAC TCACTGTGGC
    TACAAGAAGC CTCCCATGAC GTGCTTGGTG TTGATATGTG AACCAATTCC TCACCCATCA
    AACATTGAGG TCCCCTTGGA CAGTAAAACG TTCCTCAGTC GTCACAGTCT CGATATGAAA
    TTTTCCTACT GTGATGAAAG AATTACAGAG TTGATGGGAT ATGAACCAGA GGAACTCCTG
    GGTCGTTCAA TCTATGAGTA CTATCATGCG CTGGATTCTG ATCATCTGAC CAAAACACAC
    CATGATATGT TCACGAAAGG GCAGGTGACT ACGGGACAAT ACAGAATGCT TGCTAAACAA
    GGTGGCTACG TCTGGGTTGA GACTCAAGCA ACTGTTATAT ACAACACTAA GAATTCTCAA
    CCACAGTGCA TAGTATGTGT AAACTATGTG TTGAGTGGAA TTGTTCAGAA GGACTTGATA
    TTTTCCCTTG GACAAACTGA GTGTATGCTG AAACCAGTGG AGTCTCCTGA AATGAAAATG
    ACCAAAATAT TCAGCAAAGA TGACCTGGAT GATACCAACA GCCTTTTTGA AAAACTTAAG
    CAGGAACCAG ATGCTTTAAC TGTGCTGGCT CCAGCTGCTG GAGACACAAT CATCTCCCTA
    GATTTCAGCA GCAATGAGTC TGATGAACAA CAATGTGATG AAGTTCCTTT GTATAACGAT
    GTAATGCTCC CCTCATCCAG TGAGAAATTG CAGAATATAA ATATGGCAAT GTCCCCTCTA
    CCTGCCTCTG AAACTACAAA GCCACTTCGT AGCAATGCCG ATCCTGCACT CAATAGAGAA
    GTTGTATCAA AGCTGGAGCC AAACACAGAG ACACTTGAAC TTTCTTTTAC CATGCCTCAG
    GTACAAGAGC AACCAACCAG CCCTTCTGAT GCAAGTACCA GCCAAAGTTC ACCTGAGCCC
    AGTAGTCCCA ATGACTATTG CTTTGATGTG GATAATGACA TGGCTAATGA ATTCAAACTG
    GAATTAGTGG AGAAGCTCTT TGCAATAGAT ACAGAAGCAA AAAATCCATT CTCCACTCAG
    GAGACTGATT TAGACTTGGA AATGTTGGCT CCTTACATCC CAATGGATGA TGATTTCCAG
    CTACGCTCCT TTGATCAACT ATCTCCACTG GAAAGCAGTT CTTCTGGCTC TCAAAATGCA
    GCCACCATTA CCATACTTCA GCAGACTCAG ACACCATCAA CTGCTGCTGA TGAAATAAAA
    CCAGTGGCAG AACGTGTGGA TGATGTGAAG GCACTAATTG TTCCATCATC TCCTGTCCAC
    GTAATAAATG ACACGAGTAG TGCCCCTGCA TCACCTTACA GTGGAAACAG GAGTCGAACT
    GCATCTCCAA TCAGAGCAGG AAAAGGAACA TTGGAACAGA CAGAAAAATC TTGTCCAGGA
    GCACCCAGTT TGATAACAGT CACGCTGAAC AAAAGATCTA CTGCAATGGA TGAAGAACTA
    AATCCAAAGA TGCTAGCTTT GCATAATGCT CAGAGAAAAC GAAAAATGGA ACATGATGGT
    TCACTTTTTC AGGCAGTTGG AATTGGGTCT TTATTCCAGC AGACAGGTGA CCGTGGAGGA
    AACGCATCGC TTGCTTGGAA ACGTGTAAAG GCGTGCAAAA CAAACGGTCA CAATGGAGTG
    GAGCAAAAGA CAATCATTCT ACTATCAACT GACATAGCGA GTAAACTTCT AGGGCAGTCG
    ATGGATGAAA GTGGACTACC CCAACTCACG AGTTACGATT GTGAAGTAAA TGCTCCCATA
    CAAGGCAACA GAAACCTGCT ACAGGGTGAA GAACTGCTCA GAGCTCTGGA TCAAGTTAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa36742
    Clone ID Related Accession (Same CDS sequence) XM_015287264.1 , XM_015287265.2 , XM_015287265.1 , XM_015287264.2
    Accession Version XM_015287264.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2403bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product hypoxia-inducible factor 1-alpha isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015287264.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGATTAGTT CTGAACGCAG GAAAGAGAAG TCAAGAGATG CAGCCAGGTG CCGAAGAAGC 
    AAGGAATCAG AAGTGTTCTA TGAACTTGCT CATCAGCTGC CCCTTCCGCA CACTGTGAGT
    GCCCACCTTG ACAAGGCATC CATTATGAGG CTGACCATCA GCTACCTGCG CATGAGGAAG
    CTGCTGGATG CTGGTGAGCT AGAGACAGAA GCCAAAATGG AGAAGGAGCT GAACTGTTTC
    TACTTAAAAG CTCTTGATGG ATTTGTTATG GTGCTTTCTG AGGACGGGGA CATGATTTAC
    ATGTCTGAAA ACGTGAACAA GTGTATGGGA CTCACTCAGT TCGATTTGAC GGGGCACAGT
    GTATTTGATT TCACTCATCC ATGTGACCAT GAAGAGCTGA GAGAAATGCT TACACACAGA
    AATGGTCCTG TGAAAAAGGG CAAAGAGCAA AACACGGAGC GCAGCTTCTT TCTCAGAATG
    AAGTGTACGC TGACCAGCAG GGGAAGAACA GTGAATATAA AGTCTGCTAC ATGGAAGGTA
    CTGCACTGCA CTGGACACAT ACGTGTATAT GACACATGCA ATAATCAAAC TCACTGTGGC
    TACAAGAAGC CTCCCATGAC GTGCTTGGTG TTGATATGTG AACCAATTCC TCACCCATCA
    AACATTGAGG TCCCCTTGGA CAGTAAAACG TTCCTCAGTC GTCACAGTCT CGATATGAAA
    TTTTCCTACT GTGATGAAAG AATTACAGAG TTGATGGGAT ATGAACCAGA GGAACTCCTG
    GGTCGTTCAA TCTATGAGTA CTATCATGCG CTGGATTCTG ATCATCTGAC CAAAACACAC
    CATGATATGT TCACGAAAGG GCAGGTGACT ACGGGACAAT ACAGAATGCT TGCTAAACAA
    GGTGGCTACG TCTGGGTTGA GACTCAAGCA ACTGTTATAT ACAACACTAA GAATTCTCAA
    CCACAGTGCA TAGTATGTGT AAACTATGTG TTGAGTGGAA TTGTTCAGAA GGACTTGATA
    TTTTCCCTTG GACAAACTGA GTGTATGCTG AAACCAGTGG AGTCTCCTGA AATGAAAATG
    ACCAAAATAT TCAGCAAAGA TGACCTGGAT GATACCAACA GCCTTTTTGA AAAACTTAAG
    CAGGAACCAG ATGCTTTAAC TGTGCTGGCT CCAGCTGCTG GAGACACAAT CATCTCCCTA
    GATTTCAGCA GCAATGAGTC TGATGAACAA CAATGTGATG AAGTTCCTTT GTATAACGAT
    GTAATGCTCC CCTCATCCAG TGAGAAATTG CAGAATATAA ATATGGCAAT GTCCCCTCTA
    CCTGCCTCTG AAACTACAAA GCCACTTCGT AGCAATGCCG ATCCTGCACT CAATAGAGAA
    GTTGTATCAA AGCTGGAGCC AAACACAGAG ACACTTGAAC TTTCTTTTAC CATGCCTCAG
    GTACAAGAGC AACCAACCAG CCCTTCTGAT GCAAGTACCA GCCAAAGTTC ACCTGAGCCC
    AGTAGTCCCA ATGACTATTG CTTTGATGTG GATAATGACA TGGCTAATGA ATTCAAACTG
    GAATTAGTGG AGAAGCTCTT TGCAATAGAT ACAGAAGCAA AAAATCCATT CTCCACTCAG
    GAGACTGATT TAGACTTGGA AATGTTGGCT CCTTACATCC CAATGGATGA TGATTTCCAG
    CTACGCTCCT TTGATCAACT ATCTCCACTG GAAAGCAGTT CTTCTGGCTC TCAAAATGCA
    GCCACCATTA CCATACTTCA GCAGACTCAG ACACCATCAA CTGCTGCTGA TGAAATAAAA
    CCAGTGGCAG AACGTGTGGA TGATGTGAAG GCACTAATTG TTCCATCATC TCCTGTCCAC
    GTAATAAATG ACACGAGTAG TGCCCCTGCA TCACCTTACA GTGGAAACAG GAGTCGAACT
    GCATCTCCAA TCAGAGCAGG AAAAGGAACA TTGGAACAGA CAGAAAAATC TTGTCCAGGA
    GCACCCAGTT TGATAACAGT CACGCTGAAC AAAAGATCTA CTGCAATGGA TGAAGAACTA
    AATCCAAAGA TGCTAGCTTT GCATAATGCT CAGAGAAAAC GAAAAATGGA ACATGATGGT
    TCACTTTTTC AGGCAGTTGG AATTGGGTCT TTATTCCAGC AGACAGGTGA CCGTGGAGGA
    AACGCATCGC TTGCTTGGAA ACGTGTAAAG GCGTGCAAAA CAAACGGTCA CAATGGAGTG
    GAGCAAAAGA CAATCATTCT ACTATCAACT GACATAGCGA GTAAACTTCT AGGGCAGTCG
    ATGGATGAAA GTGGACTACC CCAACTCACG AGTTACGATT GTGAAGTAAA TGCTCCCATA
    CAAGGCAACA GAAACCTGCT ACAGGGTGAA GAACTGCTCA GAGCTCTGGA TCAAGTTAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015142750.1
    CDS195..2597
    Translation

    Target ORF information:

    RefSeq Version XM_015287264.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus hypoxia inducible factor 1 alpha subunit (HIF1A), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015287264.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGATTAGTT CTGAACGCAG GAAAGAGAAG TCAAGAGATG CAGCCAGGTG CCGAAGAAGC 
    AAGGAATCAG AAGTGTTCTA TGAACTTGCT CATCAGCTGC CCCTTCCGCA CACTGTGAGT
    GCCCACCTTG ACAAGGCATC CATTATGAGG CTGACCATCA GCTACCTGCG CATGAGGAAG
    CTGCTGGATG CTGGTGAGCT AGAGACAGAA GCCAAAATGG AGAAGGAGCT GAACTGTTTC
    TACTTAAAAG CTCTTGATGG ATTTGTTATG GTGCTTTCTG AGGACGGGGA CATGATTTAC
    ATGTCTGAAA ACGTGAACAA GTGTATGGGA CTCACTCAGT TCGATTTGAC GGGGCACAGT
    GTATTTGATT TCACTCATCC ATGTGACCAT GAAGAGCTGA GAGAAATGCT TACACACAGA
    AATGGTCCTG TGAAAAAGGG CAAAGAGCAA AACACGGAGC GCAGCTTCTT TCTCAGAATG
    AAGTGTACGC TGACCAGCAG GGGAAGAACA GTGAATATAA AGTCTGCTAC ATGGAAGGTA
    CTGCACTGCA CTGGACACAT ACGTGTATAT GACACATGCA ATAATCAAAC TCACTGTGGC
    TACAAGAAGC CTCCCATGAC GTGCTTGGTG TTGATATGTG AACCAATTCC TCACCCATCA
    AACATTGAGG TCCCCTTGGA CAGTAAAACG TTCCTCAGTC GTCACAGTCT CGATATGAAA
    TTTTCCTACT GTGATGAAAG AATTACAGAG TTGATGGGAT ATGAACCAGA GGAACTCCTG
    GGTCGTTCAA TCTATGAGTA CTATCATGCG CTGGATTCTG ATCATCTGAC CAAAACACAC
    CATGATATGT TCACGAAAGG GCAGGTGACT ACGGGACAAT ACAGAATGCT TGCTAAACAA
    GGTGGCTACG TCTGGGTTGA GACTCAAGCA ACTGTTATAT ACAACACTAA GAATTCTCAA
    CCACAGTGCA TAGTATGTGT AAACTATGTG TTGAGTGGAA TTGTTCAGAA GGACTTGATA
    TTTTCCCTTG GACAAACTGA GTGTATGCTG AAACCAGTGG AGTCTCCTGA AATGAAAATG
    ACCAAAATAT TCAGCAAAGA TGACCTGGAT GATACCAACA GCCTTTTTGA AAAACTTAAG
    CAGGAACCAG ATGCTTTAAC TGTGCTGGCT CCAGCTGCTG GAGACACAAT CATCTCCCTA
    GATTTCAGCA GCAATGAGTC TGATGAACAA CAATGTGATG AAGTTCCTTT GTATAACGAT
    GTAATGCTCC CCTCATCCAG TGAGAAATTG CAGAATATAA ATATGGCAAT GTCCCCTCTA
    CCTGCCTCTG AAACTACAAA GCCACTTCGT AGCAATGCCG ATCCTGCACT CAATAGAGAA
    GTTGTATCAA AGCTGGAGCC AAACACAGAG ACACTTGAAC TTTCTTTTAC CATGCCTCAG
    GTACAAGAGC AACCAACCAG CCCTTCTGAT GCAAGTACCA GCCAAAGTTC ACCTGAGCCC
    AGTAGTCCCA ATGACTATTG CTTTGATGTG GATAATGACA TGGCTAATGA ATTCAAACTG
    GAATTAGTGG AGAAGCTCTT TGCAATAGAT ACAGAAGCAA AAAATCCATT CTCCACTCAG
    GAGACTGATT TAGACTTGGA AATGTTGGCT CCTTACATCC CAATGGATGA TGATTTCCAG
    CTACGCTCCT TTGATCAACT ATCTCCACTG GAAAGCAGTT CTTCTGGCTC TCAAAATGCA
    GCCACCATTA CCATACTTCA GCAGACTCAG ACACCATCAA CTGCTGCTGA TGAAATAAAA
    CCAGTGGCAG AACGTGTGGA TGATGTGAAG GCACTAATTG TTCCATCATC TCCTGTCCAC
    GTAATAAATG ACACGAGTAG TGCCCCTGCA TCACCTTACA GTGGAAACAG GAGTCGAACT
    GCATCTCCAA TCAGAGCAGG AAAAGGAACA TTGGAACAGA CAGAAAAATC TTGTCCAGGA
    GCACCCAGTT TGATAACAGT CACGCTGAAC AAAAGATCTA CTGCAATGGA TGAAGAACTA
    AATCCAAAGA TGCTAGCTTT GCATAATGCT CAGAGAAAAC GAAAAATGGA ACATGATGGT
    TCACTTTTTC AGGCAGTTGG AATTGGGTCT TTATTCCAGC AGACAGGTGA CCGTGGAGGA
    AACGCATCGC TTGCTTGGAA ACGTGTAAAG GCGTGCAAAA CAAACGGTCA CAATGGAGTG
    GAGCAAAAGA CAATCATTCT ACTATCAACT GACATAGCGA GTAAACTTCT AGGGCAGTCG
    ATGGATGAAA GTGGACTACC CCAACTCACG AGTTACGATT GTGAAGTAAA TGCTCCCATA
    CAAGGCAACA GAAACCTGCT ACAGGGTGAA GAACTGCTCA GAGCTCTGGA TCAAGTTAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa36743
    Clone ID Related Accession (Same CDS sequence) XM_015287266.2 , XM_015287266.1
    Accession Version XM_015287266.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2259bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product hypoxia-inducible factor 1-alpha isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006092.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015287266.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAGGCTGA CCATCAGCTA CCTGCGCATG AGGAAGCTGC TGGATGCTGG TGAGCTAGAG 
    ACAGAAGCCA AAATGGAGAA GGAGCTGAAC TGTTTCTACT TAAAAGCTCT TGATGGATTT
    GTTATGGTGC TTTCTGAGGA CGGGGACATG ATTTACATGT CTGAAAACGT GAACAAGTGT
    ATGGGACTCA CTCAGTTCGA TTTGACGGGG CACAGTGTAT TTGATTTCAC TCATCCATGT
    GACCATGAAG AGCTGAGAGA AATGCTTACA CACAGAAATG GTCCTGTGAA AAAGGGCAAA
    GAGCAAAACA CGGAGCGCAG CTTCTTTCTC AGAATGAAGT GTACGCTGAC CAGCAGGGGA
    AGAACAGTGA ATATAAAGTC TGCTACATGG AAGGTACTGC ACTGCACTGG ACACATACGT
    GTATATGACA CATGCAATAA TCAAACTCAC TGTGGCTACA AGAAGCCTCC CATGACGTGC
    TTGGTGTTGA TATGTGAACC AATTCCTCAC CCATCAAACA TTGAGGTCCC CTTGGACAGT
    AAAACGTTCC TCAGTCGTCA CAGTCTCGAT ATGAAATTTT CCTACTGTGA TGAAAGAATT
    ACAGAGTTGA TGGGATATGA ACCAGAGGAA CTCCTGGGTC GTTCAATCTA TGAGTACTAT
    CATGCGCTGG ATTCTGATCA TCTGACCAAA ACACACCATG ATATGTTCAC GAAAGGGCAG
    GTGACTACGG GACAATACAG AATGCTTGCT AAACAAGGTG GCTACGTCTG GGTTGAGACT
    CAAGCAACTG TTATATACAA CACTAAGAAT TCTCAACCAC AGTGCATAGT ATGTGTAAAC
    TATGTGTTGA GTGGAATTGT TCAGAAGGAC TTGATATTTT CCCTTGGACA AACTGAGTGT
    ATGCTGAAAC CAGTGGAGTC TCCTGAAATG AAAATGACCA AAATATTCAG CAAAGATGAC
    CTGGATGATA CCAACAGCCT TTTTGAAAAA CTTAAGCAGG AACCAGATGC TTTAACTGTG
    CTGGCTCCAG CTGCTGGAGA CACAATCATC TCCCTAGATT TCAGCAGCAA TGAGTCTGAT
    GAACAACAAT GTGATGAAGT TCCTTTGTAT AACGATGTAA TGCTCCCCTC ATCCAGTGAG
    AAATTGCAGA ATATAAATAT GGCAATGTCC CCTCTACCTG CCTCTGAAAC TACAAAGCCA
    CTTCGTAGCA ATGCCGATCC TGCACTCAAT AGAGAAGTTG TATCAAAGCT GGAGCCAAAC
    ACAGAGACAC TTGAACTTTC TTTTACCATG CCTCAGGTAC AAGAGCAACC AACCAGCCCT
    TCTGATGCAA GTACCAGCCA AAGTTCACCT GAGCCCAGTA GTCCCAATGA CTATTGCTTT
    GATGTGGATA ATGACATGGC TAATGAATTC AAACTGGAAT TAGTGGAGAA GCTCTTTGCA
    ATAGATACAG AAGCAAAAAA TCCATTCTCC ACTCAGGAGA CTGATTTAGA CTTGGAAATG
    TTGGCTCCTT ACATCCCAAT GGATGATGAT TTCCAGCTAC GCTCCTTTGA TCAACTATCT
    CCACTGGAAA GCAGTTCTTC TGGCTCTCAA AATGCAGCCA CCATTACCAT ACTTCAGCAG
    ACTCAGACAC CATCAACTGC TGCTGATGAA ATAAAACCAG TGGCAGAACG TGTGGATGAT
    GTGAAGGCAC TAATTGTTCC ATCATCTCCT GTCCACGTAA TAAATGACAC GAGTAGTGCC
    CCTGCATCAC CTTACAGTGG AAACAGGAGT CGAACTGCAT CTCCAATCAG AGCAGGAAAA
    GGAACATTGG AACAGACAGA AAAATCTTGT CCAGGAGCAC CCAGTTTGAT AACAGTCACG
    CTGAACAAAA GATCTACTGC AATGGATGAA GAACTAAATC CAAAGATGCT AGCTTTGCAT
    AATGCTCAGA GAAAACGAAA AATGGAACAT GATGGTTCAC TTTTTCAGGC AGTTGGAATT
    GGGTCTTTAT TCCAGCAGAC AGGTGACCGT GGAGGAAACG CATCGCTTGC TTGGAAACGT
    GTAAAGGCGT GCAAAACAAA CGGTCACAAT GGAGTGGAGC AAAAGACAAT CATTCTACTA
    TCAACTGACA TAGCGAGTAA ACTTCTAGGG CAGTCGATGG ATGAAAGTGG ACTACCCCAA
    CTCACGAGTT ACGATTGTGA AGTAAATGCT CCCATACAAG GCAACAGAAA CCTGCTACAG
    GGTGAAGAAC TGCTCAGAGC TCTGGATCAA GTTAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015142752.1
    CDS299..2557
    Translation

    Target ORF information:

    RefSeq Version XM_015287266.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus hypoxia inducible factor 1 alpha subunit (HIF1A), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015287266.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAGGCTGA CCATCAGCTA CCTGCGCATG AGGAAGCTGC TGGATGCTGG TGAGCTAGAG 
    ACAGAAGCCA AAATGGAGAA GGAGCTGAAC TGTTTCTACT TAAAAGCTCT TGATGGATTT
    GTTATGGTGC TTTCTGAGGA CGGGGACATG ATTTACATGT CTGAAAACGT GAACAAGTGT
    ATGGGACTCA CTCAGTTCGA TTTGACGGGG CACAGTGTAT TTGATTTCAC TCATCCATGT
    GACCATGAAG AGCTGAGAGA AATGCTTACA CACAGAAATG GTCCTGTGAA AAAGGGCAAA
    GAGCAAAACA CGGAGCGCAG CTTCTTTCTC AGAATGAAGT GTACGCTGAC CAGCAGGGGA
    AGAACAGTGA ATATAAAGTC TGCTACATGG AAGGTACTGC ACTGCACTGG ACACATACGT
    GTATATGACA CATGCAATAA TCAAACTCAC TGTGGCTACA AGAAGCCTCC CATGACGTGC
    TTGGTGTTGA TATGTGAACC AATTCCTCAC CCATCAAACA TTGAGGTCCC CTTGGACAGT
    AAAACGTTCC TCAGTCGTCA CAGTCTCGAT ATGAAATTTT CCTACTGTGA TGAAAGAATT
    ACAGAGTTGA TGGGATATGA ACCAGAGGAA CTCCTGGGTC GTTCAATCTA TGAGTACTAT
    CATGCGCTGG ATTCTGATCA TCTGACCAAA ACACACCATG ATATGTTCAC GAAAGGGCAG
    GTGACTACGG GACAATACAG AATGCTTGCT AAACAAGGTG GCTACGTCTG GGTTGAGACT
    CAAGCAACTG TTATATACAA CACTAAGAAT TCTCAACCAC AGTGCATAGT ATGTGTAAAC
    TATGTGTTGA GTGGAATTGT TCAGAAGGAC TTGATATTTT CCCTTGGACA AACTGAGTGT
    ATGCTGAAAC CAGTGGAGTC TCCTGAAATG AAAATGACCA AAATATTCAG CAAAGATGAC
    CTGGATGATA CCAACAGCCT TTTTGAAAAA CTTAAGCAGG AACCAGATGC TTTAACTGTG
    CTGGCTCCAG CTGCTGGAGA CACAATCATC TCCCTAGATT TCAGCAGCAA TGAGTCTGAT
    GAACAACAAT GTGATGAAGT TCCTTTGTAT AACGATGTAA TGCTCCCCTC ATCCAGTGAG
    AAATTGCAGA ATATAAATAT GGCAATGTCC CCTCTACCTG CCTCTGAAAC TACAAAGCCA
    CTTCGTAGCA ATGCCGATCC TGCACTCAAT AGAGAAGTTG TATCAAAGCT GGAGCCAAAC
    ACAGAGACAC TTGAACTTTC TTTTACCATG CCTCAGGTAC AAGAGCAACC AACCAGCCCT
    TCTGATGCAA GTACCAGCCA AAGTTCACCT GAGCCCAGTA GTCCCAATGA CTATTGCTTT
    GATGTGGATA ATGACATGGC TAATGAATTC AAACTGGAAT TAGTGGAGAA GCTCTTTGCA
    ATAGATACAG AAGCAAAAAA TCCATTCTCC ACTCAGGAGA CTGATTTAGA CTTGGAAATG
    TTGGCTCCTT ACATCCCAAT GGATGATGAT TTCCAGCTAC GCTCCTTTGA TCAACTATCT
    CCACTGGAAA GCAGTTCTTC TGGCTCTCAA AATGCAGCCA CCATTACCAT ACTTCAGCAG
    ACTCAGACAC CATCAACTGC TGCTGATGAA ATAAAACCAG TGGCAGAACG TGTGGATGAT
    GTGAAGGCAC TAATTGTTCC ATCATCTCCT GTCCACGTAA TAAATGACAC GAGTAGTGCC
    CCTGCATCAC CTTACAGTGG AAACAGGAGT CGAACTGCAT CTCCAATCAG AGCAGGAAAA
    GGAACATTGG AACAGACAGA AAAATCTTGT CCAGGAGCAC CCAGTTTGAT AACAGTCACG
    CTGAACAAAA GATCTACTGC AATGGATGAA GAACTAAATC CAAAGATGCT AGCTTTGCAT
    AATGCTCAGA GAAAACGAAA AATGGAACAT GATGGTTCAC TTTTTCAGGC AGTTGGAATT
    GGGTCTTTAT TCCAGCAGAC AGGTGACCGT GGAGGAAACG CATCGCTTGC TTGGAAACGT
    GTAAAGGCGT GCAAAACAAA CGGTCACAAT GGAGTGGAGC AAAAGACAAT CATTCTACTA
    TCAACTGACA TAGCGAGTAA ACTTCTAGGG CAGTCGATGG ATGAAAGTGG ACTACCCCAA
    CTCACGAGTT ACGATTGTGA AGTAAATGCT CCCATACAAG GCAACAGAAA CCTGCTACAG
    GGTGAAGAAC TGCTCAGAGC TCTGGATCAA GTTAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa36743
    Clone ID Related Accession (Same CDS sequence) XM_015287266.2 , XM_015287266.1
    Accession Version XM_015287266.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2259bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product hypoxia-inducible factor 1-alpha isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455885.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAGGCTGA CCATCAGCTA CCTGCGCATG AGGAAGCTGC TGGATGCTGG TGAGCTAGAG 
    ACAGAAGCCA AAATGGAGAA GGAGCTGAAC TGTTTCTACT TAAAAGCTCT TGATGGATTT
    GTTATGGTGC TTTCTGAGGA CGGGGACATG ATTTACATGT CTGAAAACGT GAACAAGTGT
    ATGGGACTCA CTCAGTTCGA TTTGACGGGG CACAGTGTAT TTGATTTCAC TCATCCATGT
    GACCATGAAG AGCTGAGAGA AATGCTTACA CACAGAAATG GTCCTGTGAA AAAGGGCAAA
    GAGCAAAACA CGGAGCGCAG CTTCTTTCTC AGAATGAAGT GTACGCTGAC CAGCAGGGGA
    AGAACAGTGA ATATAAAGTC TGCTACATGG AAGGTACTGC ACTGCACTGG ACACATACGT
    GTATATGACA CATGCAATAA TCAAACTCAC TGTGGCTACA AGAAGCCTCC CATGACGTGC
    TTGGTGTTGA TATGTGAACC AATTCCTCAC CCATCAAACA TTGAGGTCCC CTTGGACAGT
    AAAACGTTCC TCAGTCGTCA CAGTCTCGAT ATGAAATTTT CCTACTGTGA TGAAAGAATT
    ACAGAGTTGA TGGGATATGA ACCAGAGGAA CTCCTGGGTC GTTCAATCTA TGAGTACTAT
    CATGCGCTGG ATTCTGATCA TCTGACCAAA ACACACCATG ATATGTTCAC GAAAGGGCAG
    GTGACTACGG GACAATACAG AATGCTTGCT AAACAAGGTG GCTACGTCTG GGTTGAGACT
    CAAGCAACTG TTATATACAA CACTAAGAAT TCTCAACCAC AGTGCATAGT ATGTGTAAAC
    TATGTGTTGA GTGGAATTGT TCAGAAGGAC TTGATATTTT CCCTTGGACA AACTGAGTGT
    ATGCTGAAAC CAGTGGAGTC TCCTGAAATG AAAATGACCA AAATATTCAG CAAAGATGAC
    CTGGATGATA CCAACAGCCT TTTTGAAAAA CTTAAGCAGG AACCAGATGC TTTAACTGTG
    CTGGCTCCAG CTGCTGGAGA CACAATCATC TCCCTAGATT TCAGCAGCAA TGAGTCTGAT
    GAACAACAAT GTGATGAAGT TCCTTTGTAT AACGATGTAA TGCTCCCCTC ATCCAGTGAG
    AAATTGCAGA ATATAAATAT GGCAATGTCC CCTCTACCTG CCTCTGAAAC TACAAAGCCA
    CTTCGTAGCA ATGCCGATCC TGCACTCAAT AGAGAAGTTG TATCAAAGCT GGAGCCAAAC
    ACAGAGACAC TTGAACTTTC TTTTACCATG CCTCAGGTAC AAGAGCAACC AACCAGCCCT
    TCTGATGCAA GTACCAGCCA AAGTTCACCT GAGCCCAGTA GTCCCAATGA CTATTGCTTT
    GATGTGGATA ATGACATGGC TAATGAATTC AAACTGGAAT TAGTGGAGAA GCTCTTTGCA
    ATAGATACAG AAGCAAAAAA TCCATTCTCC ACTCAGGAGA CTGATTTAGA CTTGGAAATG
    TTGGCTCCTT ACATCCCAAT GGATGATGAT TTCCAGCTAC GCTCCTTTGA TCAACTATCT
    CCACTGGAAA GCAGTTCTTC TGGCTCTCAA AATGCAGCCA CCATTACCAT ACTTCAGCAG
    ACTCAGACAC CATCAACTGC TGCTGATGAA ATAAAACCAG TGGCAGAACG TGTGGATGAT
    GTGAAGGCAC TAATTGTTCC ATCATCTCCT GTCCACGTAA TAAATGACAC GAGTAGTGCC
    CCTGCATCAC CTTACAGTGG AAACAGGAGT CGAACTGCAT CTCCAATCAG AGCAGGAAAA
    GGAACATTGG AACAGACAGA AAAATCTTGT CCAGGAGCAC CCAGTTTGAT AACAGTCACG
    CTGAACAAAA GATCTACTGC AATGGATGAA GAACTAAATC CAAAGATGCT AGCTTTGCAT
    AATGCTCAGA GAAAACGAAA AATGGAACAT GATGGTTCAC TTTTTCAGGC AGTTGGAATT
    GGGTCTTTAT TCCAGCAGAC AGGTGACCGT GGAGGAAACG CATCGCTTGC TTGGAAACGT
    GTAAAGGCGT GCAAAACAAA CGGTCACAAT GGAGTGGAGC AAAAGACAAT CATTCTACTA
    TCAACTGACA TAGCGAGTAA ACTTCTAGGG CAGTCGATGG ATGAAAGTGG ACTACCCCAA
    CTCACGAGTT ACGATTGTGA AGTAAATGCT CCCATACAAG GCAACAGAAA CCTGCTACAG
    GGTGAAGAAC TGCTCAGAGC TCTGGATCAA GTTAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015142752.1
    CDS300..2558
    Translation

    Target ORF information:

    RefSeq Version XM_015287266.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) (HIF1A), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015287266.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGAGGCTGA CCATCAGCTA CCTGCGCATG AGGAAGCTGC TGGATGCTGG TGAGCTAGAG 
    ACAGAAGCCA AAATGGAGAA GGAGCTGAAC TGTTTCTACT TAAAAGCTCT TGATGGATTT
    GTTATGGTGC TTTCTGAGGA CGGGGACATG ATTTACATGT CTGAAAACGT GAACAAGTGT
    ATGGGACTCA CTCAGTTCGA TTTGACGGGG CACAGTGTAT TTGATTTCAC TCATCCATGT
    GACCATGAAG AGCTGAGAGA AATGCTTACA CACAGAAATG GTCCTGTGAA AAAGGGCAAA
    GAGCAAAACA CGGAGCGCAG CTTCTTTCTC AGAATGAAGT GTACGCTGAC CAGCAGGGGA
    AGAACAGTGA ATATAAAGTC TGCTACATGG AAGGTACTGC ACTGCACTGG ACACATACGT
    GTATATGACA CATGCAATAA TCAAACTCAC TGTGGCTACA AGAAGCCTCC CATGACGTGC
    TTGGTGTTGA TATGTGAACC AATTCCTCAC CCATCAAACA TTGAGGTCCC CTTGGACAGT
    AAAACGTTCC TCAGTCGTCA CAGTCTCGAT ATGAAATTTT CCTACTGTGA TGAAAGAATT
    ACAGAGTTGA TGGGATATGA ACCAGAGGAA CTCCTGGGTC GTTCAATCTA TGAGTACTAT
    CATGCGCTGG ATTCTGATCA TCTGACCAAA ACACACCATG ATATGTTCAC GAAAGGGCAG
    GTGACTACGG GACAATACAG AATGCTTGCT AAACAAGGTG GCTACGTCTG GGTTGAGACT
    CAAGCAACTG TTATATACAA CACTAAGAAT TCTCAACCAC AGTGCATAGT ATGTGTAAAC
    TATGTGTTGA GTGGAATTGT TCAGAAGGAC TTGATATTTT CCCTTGGACA AACTGAGTGT
    ATGCTGAAAC CAGTGGAGTC TCCTGAAATG AAAATGACCA AAATATTCAG CAAAGATGAC
    CTGGATGATA CCAACAGCCT TTTTGAAAAA CTTAAGCAGG AACCAGATGC TTTAACTGTG
    CTGGCTCCAG CTGCTGGAGA CACAATCATC TCCCTAGATT TCAGCAGCAA TGAGTCTGAT
    GAACAACAAT GTGATGAAGT TCCTTTGTAT AACGATGTAA TGCTCCCCTC ATCCAGTGAG
    AAATTGCAGA ATATAAATAT GGCAATGTCC CCTCTACCTG CCTCTGAAAC TACAAAGCCA
    CTTCGTAGCA ATGCCGATCC TGCACTCAAT AGAGAAGTTG TATCAAAGCT GGAGCCAAAC
    ACAGAGACAC TTGAACTTTC TTTTACCATG CCTCAGGTAC AAGAGCAACC AACCAGCCCT
    TCTGATGCAA GTACCAGCCA AAGTTCACCT GAGCCCAGTA GTCCCAATGA CTATTGCTTT
    GATGTGGATA ATGACATGGC TAATGAATTC AAACTGGAAT TAGTGGAGAA GCTCTTTGCA
    ATAGATACAG AAGCAAAAAA TCCATTCTCC ACTCAGGAGA CTGATTTAGA CTTGGAAATG
    TTGGCTCCTT ACATCCCAAT GGATGATGAT TTCCAGCTAC GCTCCTTTGA TCAACTATCT
    CCACTGGAAA GCAGTTCTTC TGGCTCTCAA AATGCAGCCA CCATTACCAT ACTTCAGCAG
    ACTCAGACAC CATCAACTGC TGCTGATGAA ATAAAACCAG TGGCAGAACG TGTGGATGAT
    GTGAAGGCAC TAATTGTTCC ATCATCTCCT GTCCACGTAA TAAATGACAC GAGTAGTGCC
    CCTGCATCAC CTTACAGTGG AAACAGGAGT CGAACTGCAT CTCCAATCAG AGCAGGAAAA
    GGAACATTGG AACAGACAGA AAAATCTTGT CCAGGAGCAC CCAGTTTGAT AACAGTCACG
    CTGAACAAAA GATCTACTGC AATGGATGAA GAACTAAATC CAAAGATGCT AGCTTTGCAT
    AATGCTCAGA GAAAACGAAA AATGGAACAT GATGGTTCAC TTTTTCAGGC AGTTGGAATT
    GGGTCTTTAT TCCAGCAGAC AGGTGACCGT GGAGGAAACG CATCGCTTGC TTGGAAACGT
    GTAAAGGCGT GCAAAACAAA CGGTCACAAT GGAGTGGAGC AAAAGACAAT CATTCTACTA
    TCAACTGACA TAGCGAGTAA ACTTCTAGGG CAGTCGATGG ATGAAAGTGG ACTACCCCAA
    CTCACGAGTT ACGATTGTGA AGTAAATGCT CCCATACAAG GCAACAGAAA CCTGCTACAG
    GGTGAAGAAC TGCTCAGAGC TCTGGATCAA GTTAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.