HIF1A cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	HIF1A
Entrez Gene ID	480348
Full Name	hypoxia inducible factor 1 alpha subunit
General protein information	Preferred Names hypoxia inducible factor 1 alpha subunit Names hypoxia-inducible factor 1-alpha hypoxia-inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001287163.1	NP_001274092.1	hypoxia-inducible factor 1-alpha

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	HIF1A	XP_003639249.1
Homo sapiens (human)	HIF1A	NP_001521.1
Rattus norvegicus (Norway rat)	Hif1a	NP_077335.1
	HIF1A	XP_001099149.1
Bos taurus (cattle)	HIF1A	NP_776764.2
Mus musculus (house mouse)	Hif1a	NP_034561.2
Danio rerio (zebrafish)	hif1ab	NP_956527.1
Pan troglodytes (chimpanzee)	HIF1A	XP_001168972.1
Gallus gallus (chicken)	HIF1A	NP_989628.1

Gene Ontology
Bibliography

Related articles in PubMed

Pentosan polysulfate inhibits IL-1?-induced iNOS, c-Jun and HIF-1? upregulation in canine articular chondrocytes.
Bwalya EC, Kim S, Fang J, Wijekoon HMS, Hosoya K, Okumura M
PloS one12(5)e0177144(2017)

Analysis of Hypoxia-Inducible Factor-1? Expression Relative to Other Key Factors in Malignant Canine Mammary Tumours.
Shin JI, Lim HY, Kim HW, Seung BJ, Sur JH
Journal of comparative pathology153(2-3)101-10(2015 Aug-Oct)

Genome-wide identification and analysis of basic helix-loop-helix domains in dog, Canis lupus familiaris.
Wang XH, Wang Y, Liu AK, Liu XT, Zhou Y, Yao Q, Chen KP
Molecular genetics and genomics : MGG290(2)633-48(2015 Apr)

Hypoxia-inducible factor-1? causes renal cyst expansion through calcium-activated chloride secretion.
Buchholz B, Schley G, Faria D, Kroening S, Willam C, Schreiber R, Klanke B, Burzlaff N, Jantsch J, Kunzelmann K, Eckardt KU
Journal of the American Society of Nephrology : JASN25(3)465-74(2014 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

demonstrate for the first time that PPS is a novel inhibitor of IL-1beta-induced iNOS, c-Jun, and HIF-1alpha mRNA upregulation and iNOS protein induction which may be beneficial for prevention and treatment OA
Title: Pentosan polysulfate inhibits IL-1?-induced iNOS, c-Jun and HIF-1? upregulation in canine articular chondrocytes.

The results of this study imply that HIF-1alpha may potentially play a role in increased malignancy of canine mammary tumours
Title: Analysis of Hypoxia-Inducible Factor-1? Expression Relative to Other Key Factors in Malignant Canine Mammary Tumours.

Regional hypoxia in renal cysts contributes to cyst growth, primarily due to HIF-1alpha-dependent calcium-activated chloride secretion
Title: Hypoxia-inducible factor-1? causes renal cyst expansion through calcium-activated chloride secretion.

Results of this study indicate a similar biological role of HIF-1alpha in dogs and in humans, which may confirm suitability of the animal model in investigations on progression of tumours in humans.
Title: Expression of hypoxia-inducible factor-1? and vascular density in mammary adenomas and adenocarcinomas in bitches.

The effect of acute and chronic goniodysgenesis-related glaucoma on teh expression of various stress-inducible proteins in canine retinas is reported.
Title: Comparison of the distribution of glial fibrillary acidic protein, heat shock protein 60, and hypoxia-inducible factor-1alpha in retinas from glaucomatous and normal canine eyes.

The following HIF1A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HIF1A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCe00387	NM_001287163.1 Latest version!	Canis lupus familiaris hypoxia inducible factor 1 subunit alpha (HIF1A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HIF1A ( NM_001287163.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> NP_001274092.1 Canis lupus familiaris hypoxia inducible factor 1 subunit alpha (HIF1A), mRNA.

Price & Availability↑

CloneID	OCe00387
Clone ID Related Accession (Same CDS sequence)	NM_001287163.1
Accession Version	NM_001287163.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2472bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-04
Organism	Canis lupus familiaris(dog)
Product	hypoxia-inducible factor 1-alpha
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DN365115.1, DN441742.1, AY455802.1, AAEX03005770.1, DN266988.1, DN383577.1, DN439308.1 and DN395152.1. On Nov 30, 2013 this sequence version replaced XM_003639201.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA103936001, SAMEA103936002 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END##

ATGGAGGGCG CCGGCGGCGC GAACGACAAG AAAAAAATAA GTTCTGAACG TCGAAAAGAA 
AAGTCTAGAG ATGCAGCCAG ATCTCGGAGA AGTAAAGAGT CTGAAGTTTT TTACGAGCTT 
GCTCATCAGT TGCCACTTCC TCATAATGTG AGCTCGCATC TTGATAAGGC TTCTGTTATG 
AGGCTTACCA TCAGCTATTT GCGTGTAAGG AAGCTTCTGG ATGCTGGTGA CTTAGATATT 
GAAGATGAAA TGAAGGCACA GATGAATTGC TTTTATTTGA AAGCCCTGGA TGGTTTTGTT 
ATGGTTCTCA CAGATGATGG TGACATGATT TACATTTCTG ATAATGTGAA CAAATACATG 
GGATTGACTC AGTTTGAACT AACTGGACAC AGTGTGTTTG ATTTTACTCA TCCATGTGAC 
CATGAGGAAA TGAGAGAAAT GCTTACACAC AGAAATGGCC TTGTGAAAAA GGGTAAAGAG 
CAGAATACAC AGCGAAGTTT TTTTCTCAGA ATGAAGTGTA CCCTAACTAG CCGGGGGAGA 
ACTATGAACA TCAAGTCAGC AACATGGAAA GTACTTCACT GCACAGGCCA CATTCATGTC 
TACGATACTA ATAGTAACCA ATCTCAGTGT GGGTATAAGA AACCACCCAT GACGTGCTTG 
GTGCTGATTT GTGAACCCAT TCCTCATCCA TCAAATATTG AAATTCCTTT AGATAGCAAG 
ACTTTTCTCA GTCGTCACAG TCTGGATATG AAATTTTCTT ATTGTGATGA AAGAATTACT 
GAATTGATGG GATATGAGCC AGAAGAACTT TTGGGCCGCT CAATATATGA ATATTATCAT 
GCTTTGGACT CTGACCATCT GACCAAAACT CATCATGATA TGTTTACTAA AGGACAAGTC 
ACCACAGGAC AGTACAGGAT GCTTGCCAAA AGAGGTGGTT ATGTCTGGGT TGAAACTCAA 
GCAACTGTCA TATATAACAC TAAGAACTCT CAACCACAGT GCATTGTATG TGTAAATTAT 
GTTGTGAGTG GTATTATTCA GCACGACTTG ATTTTCTCCC TTCAACAAAC AGAATGTGTC 
CTCAAACCAG TTGAATCTTC AGATATGAAA ATGACTCAGC TATTCACCAA AGTTGAATCA 
GAAGATACAA GTAGTCTCTT TGATAAGCTT AAAAAGGAAC CTGATGCTTT AACTTTGCTG 
GCCCCAGCTG CTGGAGACAC AATCATATCT TTAGATTTCG GCAGCAATGA CACAGAAACT 
GATGACCAAC AACTTGAGGA AGTTCCGTTG TATAATGATG TAATGCTCCC CTCATCCAAC 
GAAAAATTAC AGAATATAAA TTTGGCAATG TCTCCATTAC CTGCTTCTGA AACTCCAAAG 
CCACTTCGAA GTAGTGCTGA CCCGGCACTC AATCAAGAAG TTGCGTTAAA ATTAGAGCCA 
AATCCAGAGT CACTGGAACT TTCTTTTACT ATGCCCCAGA TTCAAGATCA GCCAGCTAGT 
CCTTCTGATG GAAGCACTAG ACAAAGTTCA CCTGAGCCCA ACAGTCCCAG TGAATATTGT 
TTTGATGTGG ATAGTGATAT GGTCAATGAA TTTAAGCTTG AATTGGTAGA GAAACTTTTT 
GCTGAAGACA CAGAAGCAAA GAATCCATTT TCCACCCAGG ACACTGATTT AGACTTGGAG 
ATGTTAGCTC CTTATATCCC AATGGATGAT GACTTCCAGT TACGTTCCTT CGATCAGTTG 
TCACCACTGG AAAGCAATTC TACAAGTCCT CAAAGTGCGA GCACAATTAC AGTATTCCAG 
CCGACTCCAA TGCAAGAACC TCCTCTTACT ACTACCAGTA CCACTGCTAC CACTGATGAA 
TTAAAAACAG TGACAAAAGA TGGCATAGAA GACATTAAAA TATTGATTGC AGCTCCATCT 
CCTACCCATG TACCTAAAGT AACTACTAGT GCCACAACAT CACCATACAG TGATACTGGA 
AGTCGGACAG CCTCACCAAA CAGAGCAGGA AAAGGAGTAA TAGAACAGAC GGAAAAATCT 
CATCCAAGAA GCCCTAACGT GTTATCTGTC ACTTTGAGTC AAAGAACTAC CATTCCTGAG 
GAAGAATTAA ATCCAAAGAT ACTAGCTTTG CAGAATGCGC AGAGAAAGCG AAAAATAGAA 
CATGACGGTT CACTTTTTCA AGCAGTAGGA ATTGGAACAT TATTACAGCA GCCAGATGAT 
CGTGCAACTA CTACATCACT TTCTTGGAAA CGCGTAAAAG GATGCAAGTC TAGTGAACAG 
AATGGAATGG AGCAAAAGAC AATTATTTTA ATACCTTCTG ATTTAGCGTG TAGACTGCTG 
GGGCAATCAA TGGATGAGAG TGGATTACCA CAACTGACCA GTTATGATTG TGAAGTTAAT 
GCTCCTATAC AAGGCAGCAG AAACCTACTG CAGGGTGAAG AATTACTCAG AGCTTTGGAT 
CAAGTTAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001274092.1
CDS	291..2762
Translation	MEGAGGANDKKKISSERRKEKSRDAARSRRSKESEVFYELAHQLPLPHNVSSHLDKASVMRLTISYLRVRKLLDAGDLDIEDEMKAQMNCFYLKALDGFVMVLTDDGDMIYISDNVNKYMGLTQFELTGHSVFDFTHPCDHEEMREMLTHRNGLVKKGKEQNTQRSFFLRMKCTLTSRGRTMNIKSATWKVLHCTGHIHVYDTNSNQSQCGYKKPPMTCLVLICEPIPHPSNIEIPLDSKTFLSRHSLDMKFSYCDERITELMGYEPEELLGRSIYEYYHALDSDHLTKTHHDMFTKGQVTTGQYRMLAKRGGYVWVETQATVIYNTKNSQPQCIVCVNYVVSGIIQHDLIFSLQQTECVLKPVESSDMKMTQLFTKVESEDTSSLFDKLKKEPDALTLLAPAAGDTIISLDFGSNDTETDDQQLEEVPLYNDVMLPSSNEKLQNINLAMSPLPASETPKPLRSSADPALNQEVALKLEPNPESLELSFTMPQIQDQPASPSDGSTRQSSPEPNSPSEYCFDVDSDMVNEFKLELVEKLFAEDTEAKNPFSTQDTDLDLEMLAPYIPMDDDFQLRSFDQLSPLESNSTSPQSASTITVFQPTPMQEPPLTTTSTTATTDELKTVTKDGIEDIKILIAAPSPTHVPKVTTSATTSPYSDTGSRTASPNRAGKGVIEQTEKSHPRSPNVLSVTLSQRTTIPEEELNPKILALQNAQRKRKIEHDGSLFQAVGIGTLLQQPDDRATTTSLSWKRVKGCKSSEQNGMEQKTIILIPSDLACRLLGQSMDESGLPQLTSYDCEVNAPIQGSRNLLQGEELLRALDQVN

Target ORF information:

RefSeq Version	NM_001287163.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris hypoxia inducible factor 1 subunit alpha (HIF1A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001287163.1

ORF Insert Sequence:

ATGGAGGGCG CCGGCGGCGC GAACGACAAG AAAAAAATAA GTTCTGAACG TCGAAAAGAA 
AAGTCTAGAG ATGCAGCCAG ATCTCGGAGA AGTAAAGAGT CTGAAGTTTT TTACGAGCTT 
GCTCATCAGT TGCCACTTCC TCATAATGTG AGCTCGCATC TTGATAAGGC TTCTGTTATG 
AGGCTTACCA TCAGCTATTT GCGTGTAAGG AAGCTTCTGG ATGCTGGTGA CTTAGATATT 
GAAGATGAAA TGAAGGCACA GATGAATTGC TTTTATTTGA AAGCCCTGGA TGGTTTTGTT 
ATGGTTCTCA CAGATGATGG TGACATGATT TACATTTCTG ATAATGTGAA CAAATACATG 
GGATTGACTC AGTTTGAACT AACTGGACAC AGTGTGTTTG ATTTTACTCA TCCATGTGAC 
CATGAGGAAA TGAGAGAAAT GCTTACACAC AGAAATGGCC TTGTGAAAAA GGGTAAAGAG 
CAGAATACAC AGCGAAGTTT TTTTCTCAGA ATGAAGTGTA CCCTAACTAG CCGGGGGAGA 
ACTATGAACA TCAAGTCAGC AACATGGAAA GTACTTCACT GCACAGGCCA CATTCATGTC 
TACGATACTA ATAGTAACCA ATCTCAGTGT GGGTATAAGA AACCACCCAT GACGTGCTTG 
GTGCTGATTT GTGAACCCAT TCCTCATCCA TCAAATATTG AAATTCCTTT AGATAGCAAG 
ACTTTTCTCA GTCGTCACAG TCTGGATATG AAATTTTCTT ATTGTGATGA AAGAATTACT 
GAATTGATGG GATATGAGCC AGAAGAACTT TTGGGCCGCT CAATATATGA ATATTATCAT 
GCTTTGGACT CTGACCATCT GACCAAAACT CATCATGATA TGTTTACTAA AGGACAAGTC 
ACCACAGGAC AGTACAGGAT GCTTGCCAAA AGAGGTGGTT ATGTCTGGGT TGAAACTCAA 
GCAACTGTCA TATATAACAC TAAGAACTCT CAACCACAGT GCATTGTATG TGTAAATTAT 
GTTGTGAGTG GTATTATTCA GCACGACTTG ATTTTCTCCC TTCAACAAAC AGAATGTGTC 
CTCAAACCAG TTGAATCTTC AGATATGAAA ATGACTCAGC TATTCACCAA AGTTGAATCA 
GAAGATACAA GTAGTCTCTT TGATAAGCTT AAAAAGGAAC CTGATGCTTT AACTTTGCTG 
GCCCCAGCTG CTGGAGACAC AATCATATCT TTAGATTTCG GCAGCAATGA CACAGAAACT 
GATGACCAAC AACTTGAGGA AGTTCCGTTG TATAATGATG TAATGCTCCC CTCATCCAAC 
GAAAAATTAC AGAATATAAA TTTGGCAATG TCTCCATTAC CTGCTTCTGA AACTCCAAAG 
CCACTTCGAA GTAGTGCTGA CCCGGCACTC AATCAAGAAG TTGCGTTAAA ATTAGAGCCA 
AATCCAGAGT CACTGGAACT TTCTTTTACT ATGCCCCAGA TTCAAGATCA GCCAGCTAGT 
CCTTCTGATG GAAGCACTAG ACAAAGTTCA CCTGAGCCCA ACAGTCCCAG TGAATATTGT 
TTTGATGTGG ATAGTGATAT GGTCAATGAA TTTAAGCTTG AATTGGTAGA GAAACTTTTT 
GCTGAAGACA CAGAAGCAAA GAATCCATTT TCCACCCAGG ACACTGATTT AGACTTGGAG 
ATGTTAGCTC CTTATATCCC AATGGATGAT GACTTCCAGT TACGTTCCTT CGATCAGTTG 
TCACCACTGG AAAGCAATTC TACAAGTCCT CAAAGTGCGA GCACAATTAC AGTATTCCAG 
CCGACTCCAA TGCAAGAACC TCCTCTTACT ACTACCAGTA CCACTGCTAC CACTGATGAA 
TTAAAAACAG TGACAAAAGA TGGCATAGAA GACATTAAAA TATTGATTGC AGCTCCATCT 
CCTACCCATG TACCTAAAGT AACTACTAGT GCCACAACAT CACCATACAG TGATACTGGA 
AGTCGGACAG CCTCACCAAA CAGAGCAGGA AAAGGAGTAA TAGAACAGAC GGAAAAATCT 
CATCCAAGAA GCCCTAACGT GTTATCTGTC ACTTTGAGTC AAAGAACTAC CATTCCTGAG 
GAAGAATTAA ATCCAAAGAT ACTAGCTTTG CAGAATGCGC AGAGAAAGCG AAAAATAGAA 
CATGACGGTT CACTTTTTCA AGCAGTAGGA ATTGGAACAT TATTACAGCA GCCAGATGAT 
CGTGCAACTA CTACATCACT TTCTTGGAAA CGCGTAAAAG GATGCAAGTC TAGTGAACAG 
AATGGAATGG AGCAAAAGAC AATTATTTTA ATACCTTCTG ATTTAGCGTG TAGACTGCTG 
GGGCAATCAA TGGATGAGAG TGGATTACCA CAACTGACCA GTTATGATTG TGAAGTTAAT 
GCTCCTATAC AAGGCAGCAG AAACCTACTG CAGGGTGAAG AATTACTCAG AGCTTTGGAT 
CAAGTTAACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Pentosan polysulfate inhibits IL-1?-induced iNOS, c-Jun and HIF-1? upregulation in canine articular chondrocytes.
PloS one12(5)e0177144(2017)
Bwalya EC,Kim S,Fang J,Wijekoon HMS,Hosoya K,Okumura M

Analysis of Hypoxia-Inducible Factor-1? Expression Relative to Other Key Factors in Malignant Canine Mammary Tumours.
Journal of comparative pathology153(2-3)101-10(2015 Aug-Oct)
Shin JI,Lim HY,Kim HW,Seung BJ,Sur JH

Genome-wide identification and analysis of basic helix-loop-helix domains in dog, Canis lupus familiaris.
Molecular genetics and genomics : MGG290(2)633-48(2015 Apr)
Wang XH,Wang Y,Liu AK,Liu XT,Zhou Y,Yao Q,Chen KP

Hypoxia-inducible factor-1? causes renal cyst expansion through calcium-activated chloride secretion.
Journal of the American Society of Nephrology : JASN25(3)465-74(2014 Mar)
Buchholz B,Schley G,Faria D,Kroening S,Willam C,Schreiber R,Klanke B,Burzlaff N,Jantsch J,Kunzelmann K,Eckardt KU

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HIF1A cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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