HIF1A cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	HIF1A
Entrez Gene ID	3091
Full Name	hypoxia inducible factor 1 alpha subunit
Synonyms	HIF-1-alpha,HIF-1A,HIF-1alpha,HIF1,HIF1-ALPHA,MOP1,PASD8,bHLHe78
General protein information	Preferred Names hypoxia inducible factor 1 alpha subunit Names hypoxia-inducible factor 1-alpha ARNT interacting protein PAS domain-containing protein 8 basic-helix-loop-helix-PAS protein MOP1 class E basic helix-loop-helix protein 78 hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) hypoxia-inducible factor 1 alpha isoform I.3 hypoxia-inducible factor1alpha member of PAS protein 1 member of PAS superfamily 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 14 Map Location: 14q23.2
Summary	This gene encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in energy metabolism, angiogenesis, apoptosis, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2011].
Disorder MIM:	603348

mRNA and Protein(s)

mRNA	Protein	Name
NM_001530.3	NP_001521.1	hypoxia-inducible factor 1-alpha isoform 1
NM_001530.4	NP_001521.1	hypoxia-inducible factor 1-alpha isoform 1
NM_181054.3	NP_851397.1	hypoxia-inducible factor 1-alpha isoform 2
NM_181054.2	NP_851397.1	hypoxia-inducible factor 1-alpha isoform 2
NM_001243084.1	NP_001230013.1	hypoxia-inducible factor 1-alpha isoform 3

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	HIF1A	XP_003639249.1
Homo sapiens (human)	HIF1A	NP_001521.1
Rattus norvegicus (Norway rat)	Hif1a	NP_077335.1
	HIF1A	XP_001099149.1
Bos taurus (cattle)	HIF1A	NP_776764.2
Mus musculus (house mouse)	Hif1a	NP_034561.2
Danio rerio (zebrafish)	hif1ab	NP_956527.1
Pan troglodytes (chimpanzee)	HIF1A	XP_001168972.1
Gallus gallus (chicken)	HIF1A	NP_989628.1

Gene Ontology

The following HIF1A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HIF1A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu26981	NM_001243084.1 Latest version!	Homo sapiens hypoxia inducible factor 1 subunit alpha (HIF1A), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$339.50-$475.30 $679.00
OHu27176	NM_001530.4 Latest version!	Homo sapiens hypoxia inducible factor 1 subunit alpha (HIF1A), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu26941	NM_181054.3 Latest version!	Homo sapiens hypoxia inducible factor 1 subunit alpha (HIF1A), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
View Old Accession Versions >>
OHu27176	NM_001530.3	Homo sapiens hypoxia inducible factor 1 alpha subunit (HIF1A), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu26941	NM_181054.2	Homo sapiens hypoxia inducible factor 1 subunit alpha (HIF1A), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

HIF1A ( NM_001243084.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001230013.1 Homo sapiens hypoxia inducible factor 1 subunit alpha (HIF1A), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu26981
Clone ID Related Accession (Same CDS sequence)	NM_001243084.1
Accession Version	NM_001243084.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2553bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-20
Organism	Homo sapiens(human)
Product	hypoxia-inducible factor 1-alpha isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from FJ790247.1, CN264320.1, X72726.1 and BC012527.2. Transcript Variant: This variant (3) contains an alternate 5' terminal exon, and uses an alternate in-frame acceptor splice site at the next exon compared to variant 1. This results in a longer isoform (3, also known as isoform I.3) with a distinct N-terminus compared to isoform 1. This isoform is transcriptionally active, and is upregulated in activated human T lymphocytes (PMID:18638657). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: FJ790247.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01820697 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCTCCC AATGTCGGAG TTTGGAAAAC AAATTTGTCT TTTTAAAAGA AGGTCTAGGA 
AACTCAAAAC CTGAAGAATT GGAAGAAATC AGAATAGAAA ATGGTAGGAT AAGTTCTGAA 
CGTCGAAAAG AAAAGTCTCG AGATGCAGCC AGATCTCGGC GAAGTAAAGA ATCTGAAGTT 
TTTTATGAGC TTGCTCATCA GTTGCCACTT CCACATAATG TGAGTTCGCA TCTTGATAAG 
GCCTCTGTGA TGAGGCTTAC CATCAGCTAT TTGCGTGTGA GGAAACTTCT GGATGCTGGT 
GATTTGGATA TTGAAGATGA CATGAAAGCA CAGATGAATT GCTTTTATTT GAAAGCCTTG 
GATGGTTTTG TTATGGTTCT CACAGATGAT GGTGACATGA TTTACATTTC TGATAATGTG 
AACAAATACA TGGGATTAAC TCAGTTTGAA CTAACTGGAC ACAGTGTGTT TGATTTTACT 
CATCCATGTG ACCATGAGGA AATGAGAGAA ATGCTTACAC ACAGAAATGG CCTTGTGAAA 
AAGGGTAAAG AACAAAACAC ACAGCGAAGC TTTTTTCTCA GAATGAAGTG TACCCTAACT 
AGCCGAGGAA GAACTATGAA CATAAAGTCT GCAACATGGA AGGTATTGCA CTGCACAGGC 
CACATTCACG TATATGATAC CAACAGTAAC CAACCTCAGT GTGGGTATAA GAAACCACCT 
ATGACCTGCT TGGTGCTGAT TTGTGAACCC ATTCCTCACC CATCAAATAT TGAAATTCCT 
TTAGATAGCA AGACTTTCCT CAGTCGACAC AGCCTGGATA TGAAATTTTC TTATTGTGAT 
GAAAGAATTA CCGAATTGAT GGGATATGAG CCAGAAGAAC TTTTAGGCCG CTCAATTTAT 
GAATATTATC ATGCTTTGGA CTCTGATCAT CTGACCAAAA CTCATCATGA TATGTTTACT 
AAAGGACAAG TCACCACAGG ACAGTACAGG ATGCTTGCCA AAAGAGGTGG ATATGTCTGG 
GTTGAAACTC AAGCAACTGT CATATATAAC ACCAAGAATT CTCAACCACA GTGCATTGTA 
TGTGTGAATT ACGTTGTGAG TGGTATTATT CAGCACGACT TGATTTTCTC CCTTCAACAA 
ACAGAATGTG TCCTTAAACC GGTTGAATCT TCAGATATGA AAATGACTCA GCTATTCACC 
AAAGTTGAAT CAGAAGATAC AAGTAGCCTC TTTGACAAAC TTAAGAAGGA ACCTGATGCT 
TTAACTTTGC TGGCCCCAGC CGCTGGAGAC ACAATCATAT CTTTAGATTT TGGCAGCAAC 
GACACAGAAA CTGATGACCA GCAACTTGAG GAAGTACCAT TATATAATGA TGTAATGCTC 
CCCTCACCCA ACGAAAAATT ACAGAATATA AATTTGGCAA TGTCTCCATT ACCCACCGCT 
GAAACGCCAA AGCCACTTCG AAGTAGTGCT GACCCTGCAC TCAATCAAGA AGTTGCATTA 
AAATTAGAAC CAAATCCAGA GTCACTGGAA CTTTCTTTTA CCATGCCCCA GATTCAGGAT 
CAGACACCTA GTCCTTCCGA TGGAAGCACT AGACAAAGTT CACCTGAGCC TAATAGTCCC 
AGTGAATATT GTTTTTATGT GGATAGTGAT ATGGTCAATG AATTCAAGTT GGAATTGGTA 
GAAAAACTTT TTGCTGAAGA CACAGAAGCA AAGAACCCAT TTTCTACTCA GGACACAGAT 
TTAGACTTGG AGATGTTAGC TCCCTATATC CCAATGGATG ATGACTTCCA GTTACGTTCC 
TTCGATCAGT TGTCACCATT AGAAAGCAGT TCCGCAAGCC CTGAAAGCGC AAGTCCTCAA 
AGCACAGTTA CAGTATTCCA GCAGACTCAA ATACAAGAAC CTACTGCTAA TGCCACCACT 
ACCACTGCCA CCACTGATGA ATTAAAAACA GTGACAAAAG ACCGTATGGA AGACATTAAA 
ATATTGATTG CATCTCCATC TCCTACCCAC ATACATAAAG AAACTACTAG TGCCACATCA 
TCACCATATA GAGATACTCA AAGTCGGACA GCCTCACCAA ACAGAGCAGG AAAAGGAGTC 
ATAGAACAGA CAGAAAAATC TCATCCAAGA AGCCCTAACG TGTTATCTGT CGCTTTGAGT 
CAAAGAACTA CAGTTCCTGA GGAAGAACTA AATCCAAAGA TACTAGCTTT GCAGAATGCT 
CAGAGAAAGC GAAAAATGGA ACATGATGGT TCACTTTTTC AAGCAGTAGG AATTGGAACA 
TTATTACAGC AGCCAGACGA TCATGCAGCT ACTACATCAC TTTCTTGGAA ACGTGTAAAA 
GGATGCAAAT CTAGTGAACA GAATGGAATG GAGCAAAAGA CAATTATTTT AATACCCTCT 
GATTTAGCAT GTAGACTGCT GGGGCAATCA ATGGATGAAA GTGGATTACC ACAGCTGACC 
AGTTATGATT GTGAAGTTAA TGCTCCTATA CAAGGCAGCA GAAACCTACT GCAGGGTGAA 
GAATTACTCA GAGCTTTGGA TCAAGTTAAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001230013.1
CDS	230..2782
Translation	MSSQCRSLENKFVFLKEGLGNSKPEELEEIRIENGRISSERRKEKSRDAARSRRSKESEVFYELAHQLPLPHNVSSHLDKASVMRLTISYLRVRKLLDAGDLDIEDDMKAQMNCFYLKALDGFVMVLTDDGDMIYISDNVNKYMGLTQFELTGHSVFDFTHPCDHEEMREMLTHRNGLVKKGKEQNTQRSFFLRMKCTLTSRGRTMNIKSATWKVLHCTGHIHVYDTNSNQPQCGYKKPPMTCLVLICEPIPHPSNIEIPLDSKTFLSRHSLDMKFSYCDERITELMGYEPEELLGRSIYEYYHALDSDHLTKTHHDMFTKGQVTTGQYRMLAKRGGYVWVETQATVIYNTKNSQPQCIVCVNYVVSGIIQHDLIFSLQQTECVLKPVESSDMKMTQLFTKVESEDTSSLFDKLKKEPDALTLLAPAAGDTIISLDFGSNDTETDDQQLEEVPLYNDVMLPSPNEKLQNINLAMSPLPTAETPKPLRSSADPALNQEVALKLEPNPESLELSFTMPQIQDQTPSPSDGSTRQSSPEPNSPSEYCFYVDSDMVNEFKLELVEKLFAEDTEAKNPFSTQDTDLDLEMLAPYIPMDDDFQLRSFDQLSPLESSSASPESASPQSTVTVFQQTQIQEPTANATTTTATTDELKTVTKDRMEDIKILIASPSPTHIHKETTSATSSPYRDTQSRTASPNRAGKGVIEQTEKSHPRSPNVLSVALSQRTTVPEEELNPKILALQNAQRKRKMEHDGSLFQAVGIGTLLQQPDDHAATTSLSWKRVKGCKSSEQNGMEQKTIILIPSDLACRLLGQSMDESGLPQLTSYDCEVNAPIQGSRNLLQGEELLRALDQVN

Target ORF information:

RefSeq Version	NM_001243084.1
Organism	Homo sapiens(human)
Definition	Homo sapiens hypoxia inducible factor 1 subunit alpha (HIF1A), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001243084.1

ORF Insert Sequence:

ATGAGCTCCC AATGTCGGAG TTTGGAAAAC AAATTTGTCT TTTTAAAAGA AGGTCTAGGA 
AACTCAAAAC CTGAAGAATT GGAAGAAATC AGAATAGAAA ATGGTAGGAT AAGTTCTGAA 
CGTCGAAAAG AAAAGTCTCG AGATGCAGCC AGATCTCGGC GAAGTAAAGA ATCTGAAGTT 
TTTTATGAGC TTGCTCATCA GTTGCCACTT CCACATAATG TGAGTTCGCA TCTTGATAAG 
GCCTCTGTGA TGAGGCTTAC CATCAGCTAT TTGCGTGTGA GGAAACTTCT GGATGCTGGT 
GATTTGGATA TTGAAGATGA CATGAAAGCA CAGATGAATT GCTTTTATTT GAAAGCCTTG 
GATGGTTTTG TTATGGTTCT CACAGATGAT GGTGACATGA TTTACATTTC TGATAATGTG 
AACAAATACA TGGGATTAAC TCAGTTTGAA CTAACTGGAC ACAGTGTGTT TGATTTTACT 
CATCCATGTG ACCATGAGGA AATGAGAGAA ATGCTTACAC ACAGAAATGG CCTTGTGAAA 
AAGGGTAAAG AACAAAACAC ACAGCGAAGC TTTTTTCTCA GAATGAAGTG TACCCTAACT 
AGCCGAGGAA GAACTATGAA CATAAAGTCT GCAACATGGA AGGTATTGCA CTGCACAGGC 
CACATTCACG TATATGATAC CAACAGTAAC CAACCTCAGT GTGGGTATAA GAAACCACCT 
ATGACCTGCT TGGTGCTGAT TTGTGAACCC ATTCCTCACC CATCAAATAT TGAAATTCCT 
TTAGATAGCA AGACTTTCCT CAGTCGACAC AGCCTGGATA TGAAATTTTC TTATTGTGAT 
GAAAGAATTA CCGAATTGAT GGGATATGAG CCAGAAGAAC TTTTAGGCCG CTCAATTTAT 
GAATATTATC ATGCTTTGGA CTCTGATCAT CTGACCAAAA CTCATCATGA TATGTTTACT 
AAAGGACAAG TCACCACAGG ACAGTACAGG ATGCTTGCCA AAAGAGGTGG ATATGTCTGG 
GTTGAAACTC AAGCAACTGT CATATATAAC ACCAAGAATT CTCAACCACA GTGCATTGTA 
TGTGTGAATT ACGTTGTGAG TGGTATTATT CAGCACGACT TGATTTTCTC CCTTCAACAA 
ACAGAATGTG TCCTTAAACC GGTTGAATCT TCAGATATGA AAATGACTCA GCTATTCACC 
AAAGTTGAAT CAGAAGATAC AAGTAGCCTC TTTGACAAAC TTAAGAAGGA ACCTGATGCT 
TTAACTTTGC TGGCCCCAGC CGCTGGAGAC ACAATCATAT CTTTAGATTT TGGCAGCAAC 
GACACAGAAA CTGATGACCA GCAACTTGAG GAAGTACCAT TATATAATGA TGTAATGCTC 
CCCTCACCCA ACGAAAAATT ACAGAATATA AATTTGGCAA TGTCTCCATT ACCCACCGCT 
GAAACGCCAA AGCCACTTCG AAGTAGTGCT GACCCTGCAC TCAATCAAGA AGTTGCATTA 
AAATTAGAAC CAAATCCAGA GTCACTGGAA CTTTCTTTTA CCATGCCCCA GATTCAGGAT 
CAGACACCTA GTCCTTCCGA TGGAAGCACT AGACAAAGTT CACCTGAGCC TAATAGTCCC 
AGTGAATATT GTTTTTATGT GGATAGTGAT ATGGTCAATG AATTCAAGTT GGAATTGGTA 
GAAAAACTTT TTGCTGAAGA CACAGAAGCA AAGAACCCAT TTTCTACTCA GGACACAGAT 
TTAGACTTGG AGATGTTAGC TCCCTATATC CCAATGGATG ATGACTTCCA GTTACGTTCC 
TTCGATCAGT TGTCACCATT AGAAAGCAGT TCCGCAAGCC CTGAAAGCGC AAGTCCTCAA 
AGCACAGTTA CAGTATTCCA GCAGACTCAA ATACAAGAAC CTACTGCTAA TGCCACCACT 
ACCACTGCCA CCACTGATGA ATTAAAAACA GTGACAAAAG ACCGTATGGA AGACATTAAA 
ATATTGATTG CATCTCCATC TCCTACCCAC ATACATAAAG AAACTACTAG TGCCACATCA 
TCACCATATA GAGATACTCA AAGTCGGACA GCCTCACCAA ACAGAGCAGG AAAAGGAGTC 
ATAGAACAGA CAGAAAAATC TCATCCAAGA AGCCCTAACG TGTTATCTGT CGCTTTGAGT 
CAAAGAACTA CAGTTCCTGA GGAAGAACTA AATCCAAAGA TACTAGCTTT GCAGAATGCT 
CAGAGAAAGC GAAAAATGGA ACATGATGGT TCACTTTTTC AAGCAGTAGG AATTGGAACA 
TTATTACAGC AGCCAGACGA TCATGCAGCT ACTACATCAC TTTCTTGGAA ACGTGTAAAA 
GGATGCAAAT CTAGTGAACA GAATGGAATG GAGCAAAAGA CAATTATTTT AATACCCTCT 
GATTTAGCAT GTAGACTGCT GGGGCAATCA ATGGATGAAA GTGGATTACC ACAGCTGACC 
AGTTATGATT GTGAAGTTAA TGCTCCTATA CAAGGCAGCA GAAACCTACT GCAGGGTGAA 
GAATTACTCA GAGCTTTGGA TCAAGTTAAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HIF1A ( NM_001530.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001521.1 Homo sapiens hypoxia inducible factor 1 alpha subunit (HIF1A), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu27176
Clone ID Related Accession (Same CDS sequence)	NM_001530.3 , NM_001530.4
Accession Version	NM_001530.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2481bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-17
Organism	Homo sapiens(human)
Product	hypoxia-inducible factor 1-alpha isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC344032.1, BC012527.2 and CN264295.1. This sequence is a reference standard in the RefSeqGene project. On Jul 26, 2008 this sequence version replaced NM_001530.2. Transcript Variant: This variant (1) represents the predominant transcript, and encodes isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC012527.2, SRR1660803.74010.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGAGGGCG CCGGCGGCGC GAACGACAAG AAAAAGATAA GTTCTGAACG TCGAAAAGAA 
AAGTCTCGAG ATGCAGCCAG ATCTCGGCGA AGTAAAGAAT CTGAAGTTTT TTATGAGCTT 
GCTCATCAGT TGCCACTTCC ACATAATGTG AGTTCGCATC TTGATAAGGC CTCTGTGATG 
AGGCTTACCA TCAGCTATTT GCGTGTGAGG AAACTTCTGG ATGCTGGTGA TTTGGATATT 
GAAGATGACA TGAAAGCACA GATGAATTGC TTTTATTTGA AAGCCTTGGA TGGTTTTGTT 
ATGGTTCTCA CAGATGATGG TGACATGATT TACATTTCTG ATAATGTGAA CAAATACATG 
GGATTAACTC AGTTTGAACT AACTGGACAC AGTGTGTTTG ATTTTACTCA TCCATGTGAC 
CATGAGGAAA TGAGAGAAAT GCTTACACAC AGAAATGGCC TTGTGAAAAA GGGTAAAGAA 
CAAAACACAC AGCGAAGCTT TTTTCTCAGA ATGAAGTGTA CCCTAACTAG CCGAGGAAGA 
ACTATGAACA TAAAGTCTGC AACATGGAAG GTATTGCACT GCACAGGCCA CATTCACGTA 
TATGATACCA ACAGTAACCA ACCTCAGTGT GGGTATAAGA AACCACCTAT GACCTGCTTG 
GTGCTGATTT GTGAACCCAT TCCTCACCCA TCAAATATTG AAATTCCTTT AGATAGCAAG 
ACTTTCCTCA GTCGACACAG CCTGGATATG AAATTTTCTT ATTGTGATGA AAGAATTACC 
GAATTGATGG GATATGAGCC AGAAGAACTT TTAGGCCGCT CAATTTATGA ATATTATCAT 
GCTTTGGACT CTGATCATCT GACCAAAACT CATCATGATA TGTTTACTAA AGGACAAGTC 
ACCACAGGAC AGTACAGGAT GCTTGCCAAA AGAGGTGGAT ATGTCTGGGT TGAAACTCAA 
GCAACTGTCA TATATAACAC CAAGAATTCT CAACCACAGT GCATTGTATG TGTGAATTAC 
GTTGTGAGTG GTATTATTCA GCACGACTTG ATTTTCTCCC TTCAACAAAC AGAATGTGTC 
CTTAAACCGG TTGAATCTTC AGATATGAAA ATGACTCAGC TATTCACCAA AGTTGAATCA 
GAAGATACAA GTAGCCTCTT TGACAAACTT AAGAAGGAAC CTGATGCTTT AACTTTGCTG 
GCCCCAGCCG CTGGAGACAC AATCATATCT TTAGATTTTG GCAGCAACGA CACAGAAACT 
GATGACCAGC AACTTGAGGA AGTACCATTA TATAATGATG TAATGCTCCC CTCACCCAAC 
GAAAAATTAC AGAATATAAA TTTGGCAATG TCTCCATTAC CCACCGCTGA AACGCCAAAG 
CCACTTCGAA GTAGTGCTGA CCCTGCACTC AATCAAGAAG TTGCATTAAA ATTAGAACCA 
AATCCAGAGT CACTGGAACT TTCTTTTACC ATGCCCCAGA TTCAGGATCA GACACCTAGT 
CCTTCCGATG GAAGCACTAG ACAAAGTTCA CCTGAGCCTA ATAGTCCCAG TGAATATTGT 
TTTTATGTGG ATAGTGATAT GGTCAATGAA TTCAAGTTGG AATTGGTAGA AAAACTTTTT 
GCTGAAGACA CAGAAGCAAA GAACCCATTT TCTACTCAGG ACACAGATTT AGACTTGGAG 
ATGTTAGCTC CCTATATCCC AATGGATGAT GACTTCCAGT TACGTTCCTT CGATCAGTTG 
TCACCATTAG AAAGCAGTTC CGCAAGCCCT GAAAGCGCAA GTCCTCAAAG CACAGTTACA 
GTATTCCAGC AGACTCAAAT ACAAGAACCT ACTGCTAATG CCACCACTAC CACTGCCACC 
ACTGATGAAT TAAAAACAGT GACAAAAGAC CGTATGGAAG ACATTAAAAT ATTGATTGCA 
TCTCCATCTC CTACCCACAT ACATAAAGAA ACTACTAGTG CCACATCATC ACCATATAGA 
GATACTCAAA GTCGGACAGC CTCACCAAAC AGAGCAGGAA AAGGAGTCAT AGAACAGACA 
GAAAAATCTC ATCCAAGAAG CCCTAACGTG TTATCTGTCG CTTTGAGTCA AAGAACTACA 
GTTCCTGAGG AAGAACTAAA TCCAAAGATA CTAGCTTTGC AGAATGCTCA GAGAAAGCGA 
AAAATGGAAC ATGATGGTTC ACTTTTTCAA GCAGTAGGAA TTGGAACATT ATTACAGCAG 
CCAGACGATC ATGCAGCTAC TACATCACTT TCTTGGAAAC GTGTAAAAGG ATGCAAATCT 
AGTGAACAGA ATGGAATGGA GCAAAAGACA ATTATTTTAA TACCCTCTGA TTTAGCATGT 
AGACTGCTGG GGCAATCAAT GGATGAAAGT GGATTACCAC AGCTGACCAG TTATGATTGT 
GAAGTTAATG CTCCTATACA AGGCAGCAGA AACCTACTGC AGGGTGAAGA ATTACTCAGA 
GCTTTGGATC AAGTTAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001521.1
CDS	405..2885
Translation	MEGAGGANDKKKISSERRKEKSRDAARSRRSKESEVFYELAHQLPLPHNVSSHLDKASVMRLTISYLRVRKLLDAGDLDIEDDMKAQMNCFYLKALDGFVMVLTDDGDMIYISDNVNKYMGLTQFELTGHSVFDFTHPCDHEEMREMLTHRNGLVKKGKEQNTQRSFFLRMKCTLTSRGRTMNIKSATWKVLHCTGHIHVYDTNSNQPQCGYKKPPMTCLVLICEPIPHPSNIEIPLDSKTFLSRHSLDMKFSYCDERITELMGYEPEELLGRSIYEYYHALDSDHLTKTHHDMFTKGQVTTGQYRMLAKRGGYVWVETQATVIYNTKNSQPQCIVCVNYVVSGIIQHDLIFSLQQTECVLKPVESSDMKMTQLFTKVESEDTSSLFDKLKKEPDALTLLAPAAGDTIISLDFGSNDTETDDQQLEEVPLYNDVMLPSPNEKLQNINLAMSPLPTAETPKPLRSSADPALNQEVALKLEPNPESLELSFTMPQIQDQTPSPSDGSTRQSSPEPNSPSEYCFYVDSDMVNEFKLELVEKLFAEDTEAKNPFSTQDTDLDLEMLAPYIPMDDDFQLRSFDQLSPLESSSASPESASPQSTVTVFQQTQIQEPTANATTTTATTDELKTVTKDRMEDIKILIASPSPTHIHKETTSATSSPYRDTQSRTASPNRAGKGVIEQTEKSHPRSPNVLSVALSQRTTVPEEELNPKILALQNAQRKRKMEHDGSLFQAVGIGTLLQQPDDHAATTSLSWKRVKGCKSSEQNGMEQKTIILIPSDLACRLLGQSMDESGLPQLTSYDCEVNAPIQGSRNLLQGEELLRALDQVN

Target ORF information:

RefSeq Version	NM_001530.3
Organism	Homo sapiens(human)
Definition	Homo sapiens hypoxia inducible factor 1 alpha subunit (HIF1A), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001530.3

ORF Insert Sequence:

ATGGAGGGCG CCGGCGGCGC GAACGACAAG AAAAAGATAA GTTCTGAACG TCGAAAAGAA 
AAGTCTCGAG ATGCAGCCAG ATCTCGGCGA AGTAAAGAAT CTGAAGTTTT TTATGAGCTT 
GCTCATCAGT TGCCACTTCC ACATAATGTG AGTTCGCATC TTGATAAGGC CTCTGTGATG 
AGGCTTACCA TCAGCTATTT GCGTGTGAGG AAACTTCTGG ATGCTGGTGA TTTGGATATT 
GAAGATGACA TGAAAGCACA GATGAATTGC TTTTATTTGA AAGCCTTGGA TGGTTTTGTT 
ATGGTTCTCA CAGATGATGG TGACATGATT TACATTTCTG ATAATGTGAA CAAATACATG 
GGATTAACTC AGTTTGAACT AACTGGACAC AGTGTGTTTG ATTTTACTCA TCCATGTGAC 
CATGAGGAAA TGAGAGAAAT GCTTACACAC AGAAATGGCC TTGTGAAAAA GGGTAAAGAA 
CAAAACACAC AGCGAAGCTT TTTTCTCAGA ATGAAGTGTA CCCTAACTAG CCGAGGAAGA 
ACTATGAACA TAAAGTCTGC AACATGGAAG GTATTGCACT GCACAGGCCA CATTCACGTA 
TATGATACCA ACAGTAACCA ACCTCAGTGT GGGTATAAGA AACCACCTAT GACCTGCTTG 
GTGCTGATTT GTGAACCCAT TCCTCACCCA TCAAATATTG AAATTCCTTT AGATAGCAAG 
ACTTTCCTCA GTCGACACAG CCTGGATATG AAATTTTCTT ATTGTGATGA AAGAATTACC 
GAATTGATGG GATATGAGCC AGAAGAACTT TTAGGCCGCT CAATTTATGA ATATTATCAT 
GCTTTGGACT CTGATCATCT GACCAAAACT CATCATGATA TGTTTACTAA AGGACAAGTC 
ACCACAGGAC AGTACAGGAT GCTTGCCAAA AGAGGTGGAT ATGTCTGGGT TGAAACTCAA 
GCAACTGTCA TATATAACAC CAAGAATTCT CAACCACAGT GCATTGTATG TGTGAATTAC 
GTTGTGAGTG GTATTATTCA GCACGACTTG ATTTTCTCCC TTCAACAAAC AGAATGTGTC 
CTTAAACCGG TTGAATCTTC AGATATGAAA ATGACTCAGC TATTCACCAA AGTTGAATCA 
GAAGATACAA GTAGCCTCTT TGACAAACTT AAGAAGGAAC CTGATGCTTT AACTTTGCTG 
GCCCCAGCCG CTGGAGACAC AATCATATCT TTAGATTTTG GCAGCAACGA CACAGAAACT 
GATGACCAGC AACTTGAGGA AGTACCATTA TATAATGATG TAATGCTCCC CTCACCCAAC 
GAAAAATTAC AGAATATAAA TTTGGCAATG TCTCCATTAC CCACCGCTGA AACGCCAAAG 
CCACTTCGAA GTAGTGCTGA CCCTGCACTC AATCAAGAAG TTGCATTAAA ATTAGAACCA 
AATCCAGAGT CACTGGAACT TTCTTTTACC ATGCCCCAGA TTCAGGATCA GACACCTAGT 
CCTTCCGATG GAAGCACTAG ACAAAGTTCA CCTGAGCCTA ATAGTCCCAG TGAATATTGT 
TTTTATGTGG ATAGTGATAT GGTCAATGAA TTCAAGTTGG AATTGGTAGA AAAACTTTTT 
GCTGAAGACA CAGAAGCAAA GAACCCATTT TCTACTCAGG ACACAGATTT AGACTTGGAG 
ATGTTAGCTC CCTATATCCC AATGGATGAT GACTTCCAGT TACGTTCCTT CGATCAGTTG 
TCACCATTAG AAAGCAGTTC CGCAAGCCCT GAAAGCGCAA GTCCTCAAAG CACAGTTACA 
GTATTCCAGC AGACTCAAAT ACAAGAACCT ACTGCTAATG CCACCACTAC CACTGCCACC 
ACTGATGAAT TAAAAACAGT GACAAAAGAC CGTATGGAAG ACATTAAAAT ATTGATTGCA 
TCTCCATCTC CTACCCACAT ACATAAAGAA ACTACTAGTG CCACATCATC ACCATATAGA 
GATACTCAAA GTCGGACAGC CTCACCAAAC AGAGCAGGAA AAGGAGTCAT AGAACAGACA 
GAAAAATCTC ATCCAAGAAG CCCTAACGTG TTATCTGTCG CTTTGAGTCA AAGAACTACA 
GTTCCTGAGG AAGAACTAAA TCCAAAGATA CTAGCTTTGC AGAATGCTCA GAGAAAGCGA 
AAAATGGAAC ATGATGGTTC ACTTTTTCAA GCAGTAGGAA TTGGAACATT ATTACAGCAG 
CCAGACGATC ATGCAGCTAC TACATCACTT TCTTGGAAAC GTGTAAAAGG ATGCAAATCT 
AGTGAACAGA ATGGAATGGA GCAAAAGACA ATTATTTTAA TACCCTCTGA TTTAGCATGT 
AGACTGCTGG GGCAATCAAT GGATGAAAGT GGATTACCAC AGCTGACCAG TTATGATTGT 
GAAGTTAATG CTCCTATACA AGGCAGCAGA AACCTACTGC AGGGTGAAGA ATTACTCAGA 
GCTTTGGATC AAGTTAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HIF1A ( NM_001530.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001521.1 Homo sapiens hypoxia inducible factor 1 subunit alpha (HIF1A), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu27176
Clone ID Related Accession (Same CDS sequence)	NM_001530.3 , NM_001530.4
Accession Version	NM_001530.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2481bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	hypoxia-inducible factor 1-alpha isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC344032.1, BC012527.2 and CN264295.1. This sequence is a reference standard in the RefSeqGene project. On Dec 7, 2018 this sequence version replaced NM_001530.3. Transcript Variant: This variant (1) represents the predominant transcript, and encodes isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.144807.1, SRR1660805.134018.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000337138.9/ ENSP00000338018.4 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAGGGCG CCGGCGGCGC GAACGACAAG AAAAAGATAA GTTCTGAACG TCGAAAAGAA 
AAGTCTCGAG ATGCAGCCAG ATCTCGGCGA AGTAAAGAAT CTGAAGTTTT TTATGAGCTT 
GCTCATCAGT TGCCACTTCC ACATAATGTG AGTTCGCATC TTGATAAGGC CTCTGTGATG 
AGGCTTACCA TCAGCTATTT GCGTGTGAGG AAACTTCTGG ATGCTGGTGA TTTGGATATT 
GAAGATGACA TGAAAGCACA GATGAATTGC TTTTATTTGA AAGCCTTGGA TGGTTTTGTT 
ATGGTTCTCA CAGATGATGG TGACATGATT TACATTTCTG ATAATGTGAA CAAATACATG 
GGATTAACTC AGTTTGAACT AACTGGACAC AGTGTGTTTG ATTTTACTCA TCCATGTGAC 
CATGAGGAAA TGAGAGAAAT GCTTACACAC AGAAATGGCC TTGTGAAAAA GGGTAAAGAA 
CAAAACACAC AGCGAAGCTT TTTTCTCAGA ATGAAGTGTA CCCTAACTAG CCGAGGAAGA 
ACTATGAACA TAAAGTCTGC AACATGGAAG GTATTGCACT GCACAGGCCA CATTCACGTA 
TATGATACCA ACAGTAACCA ACCTCAGTGT GGGTATAAGA AACCACCTAT GACCTGCTTG 
GTGCTGATTT GTGAACCCAT TCCTCACCCA TCAAATATTG AAATTCCTTT AGATAGCAAG 
ACTTTCCTCA GTCGACACAG CCTGGATATG AAATTTTCTT ATTGTGATGA AAGAATTACC 
GAATTGATGG GATATGAGCC AGAAGAACTT TTAGGCCGCT CAATTTATGA ATATTATCAT 
GCTTTGGACT CTGATCATCT GACCAAAACT CATCATGATA TGTTTACTAA AGGACAAGTC 
ACCACAGGAC AGTACAGGAT GCTTGCCAAA AGAGGTGGAT ATGTCTGGGT TGAAACTCAA 
GCAACTGTCA TATATAACAC CAAGAATTCT CAACCACAGT GCATTGTATG TGTGAATTAC 
GTTGTGAGTG GTATTATTCA GCACGACTTG ATTTTCTCCC TTCAACAAAC AGAATGTGTC 
CTTAAACCGG TTGAATCTTC AGATATGAAA ATGACTCAGC TATTCACCAA AGTTGAATCA 
GAAGATACAA GTAGCCTCTT TGACAAACTT AAGAAGGAAC CTGATGCTTT AACTTTGCTG 
GCCCCAGCCG CTGGAGACAC AATCATATCT TTAGATTTTG GCAGCAACGA CACAGAAACT 
GATGACCAGC AACTTGAGGA AGTACCATTA TATAATGATG TAATGCTCCC CTCACCCAAC 
GAAAAATTAC AGAATATAAA TTTGGCAATG TCTCCATTAC CCACCGCTGA AACGCCAAAG 
CCACTTCGAA GTAGTGCTGA CCCTGCACTC AATCAAGAAG TTGCATTAAA ATTAGAACCA 
AATCCAGAGT CACTGGAACT TTCTTTTACC ATGCCCCAGA TTCAGGATCA GACACCTAGT 
CCTTCCGATG GAAGCACTAG ACAAAGTTCA CCTGAGCCTA ATAGTCCCAG TGAATATTGT 
TTTTATGTGG ATAGTGATAT GGTCAATGAA TTCAAGTTGG AATTGGTAGA AAAACTTTTT 
GCTGAAGACA CAGAAGCAAA GAACCCATTT TCTACTCAGG ACACAGATTT AGACTTGGAG 
ATGTTAGCTC CCTATATCCC AATGGATGAT GACTTCCAGT TACGTTCCTT CGATCAGTTG 
TCACCATTAG AAAGCAGTTC CGCAAGCCCT GAAAGCGCAA GTCCTCAAAG CACAGTTACA 
GTATTCCAGC AGACTCAAAT ACAAGAACCT ACTGCTAATG CCACCACTAC CACTGCCACC 
ACTGATGAAT TAAAAACAGT GACAAAAGAC CGTATGGAAG ACATTAAAAT ATTGATTGCA 
TCTCCATCTC CTACCCACAT ACATAAAGAA ACTACTAGTG CCACATCATC ACCATATAGA 
GATACTCAAA GTCGGACAGC CTCACCAAAC AGAGCAGGAA AAGGAGTCAT AGAACAGACA 
GAAAAATCTC ATCCAAGAAG CCCTAACGTG TTATCTGTCG CTTTGAGTCA AAGAACTACA 
GTTCCTGAGG AAGAACTAAA TCCAAAGATA CTAGCTTTGC AGAATGCTCA GAGAAAGCGA 
AAAATGGAAC ATGATGGTTC ACTTTTTCAA GCAGTAGGAA TTGGAACATT ATTACAGCAG 
CCAGACGATC ATGCAGCTAC TACATCACTT TCTTGGAAAC GTGTAAAAGG ATGCAAATCT 
AGTGAACAGA ATGGAATGGA GCAAAAGACA ATTATTTTAA TACCCTCTGA TTTAGCATGT 
AGACTGCTGG GGCAATCAAT GGATGAAAGT GGATTACCAC AGCTGACCAG TTATGATTGT 
GAAGTTAATG CTCCTATACA AGGCAGCAGA AACCTACTGC AGGGTGAAGA ATTACTCAGA 
GCTTTGGATC AAGTTAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001521.1
CDS	293..2773
Translation	MEGAGGANDKKKISSERRKEKSRDAARSRRSKESEVFYELAHQLPLPHNVSSHLDKASVMRLTISYLRVRKLLDAGDLDIEDDMKAQMNCFYLKALDGFVMVLTDDGDMIYISDNVNKYMGLTQFELTGHSVFDFTHPCDHEEMREMLTHRNGLVKKGKEQNTQRSFFLRMKCTLTSRGRTMNIKSATWKVLHCTGHIHVYDTNSNQPQCGYKKPPMTCLVLICEPIPHPSNIEIPLDSKTFLSRHSLDMKFSYCDERITELMGYEPEELLGRSIYEYYHALDSDHLTKTHHDMFTKGQVTTGQYRMLAKRGGYVWVETQATVIYNTKNSQPQCIVCVNYVVSGIIQHDLIFSLQQTECVLKPVESSDMKMTQLFTKVESEDTSSLFDKLKKEPDALTLLAPAAGDTIISLDFGSNDTETDDQQLEEVPLYNDVMLPSPNEKLQNINLAMSPLPTAETPKPLRSSADPALNQEVALKLEPNPESLELSFTMPQIQDQTPSPSDGSTRQSSPEPNSPSEYCFYVDSDMVNEFKLELVEKLFAEDTEAKNPFSTQDTDLDLEMLAPYIPMDDDFQLRSFDQLSPLESSSASPESASPQSTVTVFQQTQIQEPTANATTTTATTDELKTVTKDRMEDIKILIASPSPTHIHKETTSATSSPYRDTQSRTASPNRAGKGVIEQTEKSHPRSPNVLSVALSQRTTVPEEELNPKILALQNAQRKRKMEHDGSLFQAVGIGTLLQQPDDHAATTSLSWKRVKGCKSSEQNGMEQKTIILIPSDLACRLLGQSMDESGLPQLTSYDCEVNAPIQGSRNLLQGEELLRALDQVN

Target ORF information:

RefSeq Version	NM_001530.4
Organism	Homo sapiens(human)
Definition	Homo sapiens hypoxia inducible factor 1 subunit alpha (HIF1A), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001530.4

ORF Insert Sequence:

ATGGAGGGCG CCGGCGGCGC GAACGACAAG AAAAAGATAA GTTCTGAACG TCGAAAAGAA 
AAGTCTCGAG ATGCAGCCAG ATCTCGGCGA AGTAAAGAAT CTGAAGTTTT TTATGAGCTT 
GCTCATCAGT TGCCACTTCC ACATAATGTG AGTTCGCATC TTGATAAGGC CTCTGTGATG 
AGGCTTACCA TCAGCTATTT GCGTGTGAGG AAACTTCTGG ATGCTGGTGA TTTGGATATT 
GAAGATGACA TGAAAGCACA GATGAATTGC TTTTATTTGA AAGCCTTGGA TGGTTTTGTT 
ATGGTTCTCA CAGATGATGG TGACATGATT TACATTTCTG ATAATGTGAA CAAATACATG 
GGATTAACTC AGTTTGAACT AACTGGACAC AGTGTGTTTG ATTTTACTCA TCCATGTGAC 
CATGAGGAAA TGAGAGAAAT GCTTACACAC AGAAATGGCC TTGTGAAAAA GGGTAAAGAA 
CAAAACACAC AGCGAAGCTT TTTTCTCAGA ATGAAGTGTA CCCTAACTAG CCGAGGAAGA 
ACTATGAACA TAAAGTCTGC AACATGGAAG GTATTGCACT GCACAGGCCA CATTCACGTA 
TATGATACCA ACAGTAACCA ACCTCAGTGT GGGTATAAGA AACCACCTAT GACCTGCTTG 
GTGCTGATTT GTGAACCCAT TCCTCACCCA TCAAATATTG AAATTCCTTT AGATAGCAAG 
ACTTTCCTCA GTCGACACAG CCTGGATATG AAATTTTCTT ATTGTGATGA AAGAATTACC 
GAATTGATGG GATATGAGCC AGAAGAACTT TTAGGCCGCT CAATTTATGA ATATTATCAT 
GCTTTGGACT CTGATCATCT GACCAAAACT CATCATGATA TGTTTACTAA AGGACAAGTC 
ACCACAGGAC AGTACAGGAT GCTTGCCAAA AGAGGTGGAT ATGTCTGGGT TGAAACTCAA 
GCAACTGTCA TATATAACAC CAAGAATTCT CAACCACAGT GCATTGTATG TGTGAATTAC 
GTTGTGAGTG GTATTATTCA GCACGACTTG ATTTTCTCCC TTCAACAAAC AGAATGTGTC 
CTTAAACCGG TTGAATCTTC AGATATGAAA ATGACTCAGC TATTCACCAA AGTTGAATCA 
GAAGATACAA GTAGCCTCTT TGACAAACTT AAGAAGGAAC CTGATGCTTT AACTTTGCTG 
GCCCCAGCCG CTGGAGACAC AATCATATCT TTAGATTTTG GCAGCAACGA CACAGAAACT 
GATGACCAGC AACTTGAGGA AGTACCATTA TATAATGATG TAATGCTCCC CTCACCCAAC 
GAAAAATTAC AGAATATAAA TTTGGCAATG TCTCCATTAC CCACCGCTGA AACGCCAAAG 
CCACTTCGAA GTAGTGCTGA CCCTGCACTC AATCAAGAAG TTGCATTAAA ATTAGAACCA 
AATCCAGAGT CACTGGAACT TTCTTTTACC ATGCCCCAGA TTCAGGATCA GACACCTAGT 
CCTTCCGATG GAAGCACTAG ACAAAGTTCA CCTGAGCCTA ATAGTCCCAG TGAATATTGT 
TTTTATGTGG ATAGTGATAT GGTCAATGAA TTCAAGTTGG AATTGGTAGA AAAACTTTTT 
GCTGAAGACA CAGAAGCAAA GAACCCATTT TCTACTCAGG ACACAGATTT AGACTTGGAG 
ATGTTAGCTC CCTATATCCC AATGGATGAT GACTTCCAGT TACGTTCCTT CGATCAGTTG 
TCACCATTAG AAAGCAGTTC CGCAAGCCCT GAAAGCGCAA GTCCTCAAAG CACAGTTACA 
GTATTCCAGC AGACTCAAAT ACAAGAACCT ACTGCTAATG CCACCACTAC CACTGCCACC 
ACTGATGAAT TAAAAACAGT GACAAAAGAC CGTATGGAAG ACATTAAAAT ATTGATTGCA 
TCTCCATCTC CTACCCACAT ACATAAAGAA ACTACTAGTG CCACATCATC ACCATATAGA 
GATACTCAAA GTCGGACAGC CTCACCAAAC AGAGCAGGAA AAGGAGTCAT AGAACAGACA 
GAAAAATCTC ATCCAAGAAG CCCTAACGTG TTATCTGTCG CTTTGAGTCA AAGAACTACA 
GTTCCTGAGG AAGAACTAAA TCCAAAGATA CTAGCTTTGC AGAATGCTCA GAGAAAGCGA 
AAAATGGAAC ATGATGGTTC ACTTTTTCAA GCAGTAGGAA TTGGAACATT ATTACAGCAG 
CCAGACGATC ATGCAGCTAC TACATCACTT TCTTGGAAAC GTGTAAAAGG ATGCAAATCT 
AGTGAACAGA ATGGAATGGA GCAAAAGACA ATTATTTTAA TACCCTCTGA TTTAGCATGT 
AGACTGCTGG GGCAATCAAT GGATGAAAGT GGATTACCAC AGCTGACCAG TTATGATTGT 
GAAGTTAATG CTCCTATACA AGGCAGCAGA AACCTACTGC AGGGTGAAGA ATTACTCAGA 
GCTTTGGATC AAGTTAACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HIF1A ( NM_181054.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_851397.1 Homo sapiens hypoxia inducible factor 1 subunit alpha (HIF1A), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu26941
Clone ID Related Accession (Same CDS sequence)	NM_181054.3 , NM_181054.2
Accession Version	NM_181054.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	hypoxia-inducible factor 1-alpha isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC344032.1, AB073325.1, CN264295.1 and BC012527.2. On Jun 4, 2019 this sequence version replaced NM_181054.2. Transcript Variant: This variant (2) lacks the penultimate coding exon compared to variant 1. This results in a frame-shift, and a shorter isoform (2) with a distinct C-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.250908.1, SRR1660807.224891.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGAGGGCG CCGGCGGCGC GAACGACAAG AAAAAGATAA GTTCTGAACG TCGAAAAGAA 
AAGTCTCGAG ATGCAGCCAG ATCTCGGCGA AGTAAAGAAT CTGAAGTTTT TTATGAGCTT 
GCTCATCAGT TGCCACTTCC ACATAATGTG AGTTCGCATC TTGATAAGGC CTCTGTGATG 
AGGCTTACCA TCAGCTATTT GCGTGTGAGG AAACTTCTGG ATGCTGGTGA TTTGGATATT 
GAAGATGACA TGAAAGCACA GATGAATTGC TTTTATTTGA AAGCCTTGGA TGGTTTTGTT 
ATGGTTCTCA CAGATGATGG TGACATGATT TACATTTCTG ATAATGTGAA CAAATACATG 
GGATTAACTC AGTTTGAACT AACTGGACAC AGTGTGTTTG ATTTTACTCA TCCATGTGAC 
CATGAGGAAA TGAGAGAAAT GCTTACACAC AGAAATGGCC TTGTGAAAAA GGGTAAAGAA 
CAAAACACAC AGCGAAGCTT TTTTCTCAGA ATGAAGTGTA CCCTAACTAG CCGAGGAAGA 
ACTATGAACA TAAAGTCTGC AACATGGAAG GTATTGCACT GCACAGGCCA CATTCACGTA 
TATGATACCA ACAGTAACCA ACCTCAGTGT GGGTATAAGA AACCACCTAT GACCTGCTTG 
GTGCTGATTT GTGAACCCAT TCCTCACCCA TCAAATATTG AAATTCCTTT AGATAGCAAG 
ACTTTCCTCA GTCGACACAG CCTGGATATG AAATTTTCTT ATTGTGATGA AAGAATTACC 
GAATTGATGG GATATGAGCC AGAAGAACTT TTAGGCCGCT CAATTTATGA ATATTATCAT 
GCTTTGGACT CTGATCATCT GACCAAAACT CATCATGATA TGTTTACTAA AGGACAAGTC 
ACCACAGGAC AGTACAGGAT GCTTGCCAAA AGAGGTGGAT ATGTCTGGGT TGAAACTCAA 
GCAACTGTCA TATATAACAC CAAGAATTCT CAACCACAGT GCATTGTATG TGTGAATTAC 
GTTGTGAGTG GTATTATTCA GCACGACTTG ATTTTCTCCC TTCAACAAAC AGAATGTGTC 
CTTAAACCGG TTGAATCTTC AGATATGAAA ATGACTCAGC TATTCACCAA AGTTGAATCA 
GAAGATACAA GTAGCCTCTT TGACAAACTT AAGAAGGAAC CTGATGCTTT AACTTTGCTG 
GCCCCAGCCG CTGGAGACAC AATCATATCT TTAGATTTTG GCAGCAACGA CACAGAAACT 
GATGACCAGC AACTTGAGGA AGTACCATTA TATAATGATG TAATGCTCCC CTCACCCAAC 
GAAAAATTAC AGAATATAAA TTTGGCAATG TCTCCATTAC CCACCGCTGA AACGCCAAAG 
CCACTTCGAA GTAGTGCTGA CCCTGCACTC AATCAAGAAG TTGCATTAAA ATTAGAACCA 
AATCCAGAGT CACTGGAACT TTCTTTTACC ATGCCCCAGA TTCAGGATCA GACACCTAGT 
CCTTCCGATG GAAGCACTAG ACAAAGTTCA CCTGAGCCTA ATAGTCCCAG TGAATATTGT 
TTTTATGTGG ATAGTGATAT GGTCAATGAA TTCAAGTTGG AATTGGTAGA AAAACTTTTT 
GCTGAAGACA CAGAAGCAAA GAACCCATTT TCTACTCAGG ACACAGATTT AGACTTGGAG 
ATGTTAGCTC CCTATATCCC AATGGATGAT GACTTCCAGT TACGTTCCTT CGATCAGTTG 
TCACCATTAG AAAGCAGTTC CGCAAGCCCT GAAAGCGCAA GTCCTCAAAG CACAGTTACA 
GTATTCCAGC AGACTCAAAT ACAAGAACCT ACTGCTAATG CCACCACTAC CACTGCCACC 
ACTGATGAAT TAAAAACAGT GACAAAAGAC CGTATGGAAG ACATTAAAAT ATTGATTGCA 
TCTCCATCTC CTACCCACAT ACATAAAGAA ACTACTAGTG CCACATCATC ACCATATAGA 
GATACTCAAA GTCGGACAGC CTCACCAAAC AGAGCAGGAA AAGGAGTCAT AGAACAGACA 
GAAAAATCTC ATCCAAGAAG CCCTAACGTG TTATCTGTCG CTTTGAGTCA AAGAACTACA 
GTTCCTGAGG AAGAACTAAA TCCAAAGATA CTAGCTTTGC AGAATGCTCA GAGAAAGCGA 
AAAATGGAAC ATGATGGTTC ACTTTTTCAA GCAGTAGGAA TTATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_851397.1
CDS	293..2500
Translation	MEGAGGANDKKKISSERRKEKSRDAARSRRSKESEVFYELAHQLPLPHNVSSHLDKASVMRLTISYLRVRKLLDAGDLDIEDDMKAQMNCFYLKALDGFVMVLTDDGDMIYISDNVNKYMGLTQFELTGHSVFDFTHPCDHEEMREMLTHRNGLVKKGKEQNTQRSFFLRMKCTLTSRGRTMNIKSATWKVLHCTGHIHVYDTNSNQPQCGYKKPPMTCLVLICEPIPHPSNIEIPLDSKTFLSRHSLDMKFSYCDERITELMGYEPEELLGRSIYEYYHALDSDHLTKTHHDMFTKGQVTTGQYRMLAKRGGYVWVETQATVIYNTKNSQPQCIVCVNYVVSGIIQHDLIFSLQQTECVLKPVESSDMKMTQLFTKVESEDTSSLFDKLKKEPDALTLLAPAAGDTIISLDFGSNDTETDDQQLEEVPLYNDVMLPSPNEKLQNINLAMSPLPTAETPKPLRSSADPALNQEVALKLEPNPESLELSFTMPQIQDQTPSPSDGSTRQSSPEPNSPSEYCFYVDSDMVNEFKLELVEKLFAEDTEAKNPFSTQDTDLDLEMLAPYIPMDDDFQLRSFDQLSPLESSSASPESASPQSTVTVFQQTQIQEPTANATTTTATTDELKTVTKDRMEDIKILIASPSPTHIHKETTSATSSPYRDTQSRTASPNRAGKGVIEQTEKSHPRSPNVLSVALSQRTTVPEEELNPKILALQNAQRKRKMEHDGSLFQAVGII

Target ORF information:

RefSeq Version	NM_181054.3
Organism	Homo sapiens(human)
Definition	Homo sapiens hypoxia inducible factor 1 subunit alpha (HIF1A), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_181054.3

ORF Insert Sequence:

ATGGAGGGCG CCGGCGGCGC GAACGACAAG AAAAAGATAA GTTCTGAACG TCGAAAAGAA 
AAGTCTCGAG ATGCAGCCAG ATCTCGGCGA AGTAAAGAAT CTGAAGTTTT TTATGAGCTT 
GCTCATCAGT TGCCACTTCC ACATAATGTG AGTTCGCATC TTGATAAGGC CTCTGTGATG 
AGGCTTACCA TCAGCTATTT GCGTGTGAGG AAACTTCTGG ATGCTGGTGA TTTGGATATT 
GAAGATGACA TGAAAGCACA GATGAATTGC TTTTATTTGA AAGCCTTGGA TGGTTTTGTT 
ATGGTTCTCA CAGATGATGG TGACATGATT TACATTTCTG ATAATGTGAA CAAATACATG 
GGATTAACTC AGTTTGAACT AACTGGACAC AGTGTGTTTG ATTTTACTCA TCCATGTGAC 
CATGAGGAAA TGAGAGAAAT GCTTACACAC AGAAATGGCC TTGTGAAAAA GGGTAAAGAA 
CAAAACACAC AGCGAAGCTT TTTTCTCAGA ATGAAGTGTA CCCTAACTAG CCGAGGAAGA 
ACTATGAACA TAAAGTCTGC AACATGGAAG GTATTGCACT GCACAGGCCA CATTCACGTA 
TATGATACCA ACAGTAACCA ACCTCAGTGT GGGTATAAGA AACCACCTAT GACCTGCTTG 
GTGCTGATTT GTGAACCCAT TCCTCACCCA TCAAATATTG AAATTCCTTT AGATAGCAAG 
ACTTTCCTCA GTCGACACAG CCTGGATATG AAATTTTCTT ATTGTGATGA AAGAATTACC 
GAATTGATGG GATATGAGCC AGAAGAACTT TTAGGCCGCT CAATTTATGA ATATTATCAT 
GCTTTGGACT CTGATCATCT GACCAAAACT CATCATGATA TGTTTACTAA AGGACAAGTC 
ACCACAGGAC AGTACAGGAT GCTTGCCAAA AGAGGTGGAT ATGTCTGGGT TGAAACTCAA 
GCAACTGTCA TATATAACAC CAAGAATTCT CAACCACAGT GCATTGTATG TGTGAATTAC 
GTTGTGAGTG GTATTATTCA GCACGACTTG ATTTTCTCCC TTCAACAAAC AGAATGTGTC 
CTTAAACCGG TTGAATCTTC AGATATGAAA ATGACTCAGC TATTCACCAA AGTTGAATCA 
GAAGATACAA GTAGCCTCTT TGACAAACTT AAGAAGGAAC CTGATGCTTT AACTTTGCTG 
GCCCCAGCCG CTGGAGACAC AATCATATCT TTAGATTTTG GCAGCAACGA CACAGAAACT 
GATGACCAGC AACTTGAGGA AGTACCATTA TATAATGATG TAATGCTCCC CTCACCCAAC 
GAAAAATTAC AGAATATAAA TTTGGCAATG TCTCCATTAC CCACCGCTGA AACGCCAAAG 
CCACTTCGAA GTAGTGCTGA CCCTGCACTC AATCAAGAAG TTGCATTAAA ATTAGAACCA 
AATCCAGAGT CACTGGAACT TTCTTTTACC ATGCCCCAGA TTCAGGATCA GACACCTAGT 
CCTTCCGATG GAAGCACTAG ACAAAGTTCA CCTGAGCCTA ATAGTCCCAG TGAATATTGT 
TTTTATGTGG ATAGTGATAT GGTCAATGAA TTCAAGTTGG AATTGGTAGA AAAACTTTTT 
GCTGAAGACA CAGAAGCAAA GAACCCATTT TCTACTCAGG ACACAGATTT AGACTTGGAG 
ATGTTAGCTC CCTATATCCC AATGGATGAT GACTTCCAGT TACGTTCCTT CGATCAGTTG 
TCACCATTAG AAAGCAGTTC CGCAAGCCCT GAAAGCGCAA GTCCTCAAAG CACAGTTACA 
GTATTCCAGC AGACTCAAAT ACAAGAACCT ACTGCTAATG CCACCACTAC CACTGCCACC 
ACTGATGAAT TAAAAACAGT GACAAAAGAC CGTATGGAAG ACATTAAAAT ATTGATTGCA 
TCTCCATCTC CTACCCACAT ACATAAAGAA ACTACTAGTG CCACATCATC ACCATATAGA 
GATACTCAAA GTCGGACAGC CTCACCAAAC AGAGCAGGAA AAGGAGTCAT AGAACAGACA 
GAAAAATCTC ATCCAAGAAG CCCTAACGTG TTATCTGTCG CTTTGAGTCA AAGAACTACA 
GTTCCTGAGG AAGAACTAAA TCCAAAGATA CTAGCTTTGC AGAATGCTCA GAGAAAGCGA 
AAAATGGAAC ATGATGGTTC ACTTTTTCAA GCAGTAGGAA TTATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

HIF1A ( NM_181054.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_851397.1 Homo sapiens hypoxia inducible factor 1 subunit alpha (HIF1A), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu26941
Clone ID Related Accession (Same CDS sequence)	NM_181054.3 , NM_181054.2
Accession Version	NM_181054.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-11
Organism	Homo sapiens(human)
Product	hypoxia-inducible factor 1-alpha isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC344032.1, AB073325.1, CN264295.1 and BC012527.2. On Jul 26, 2008 this sequence version replaced NM_181054.1. Transcript Variant: This variant (2) lacks the penultimate coding exon compared to variant 1. This results in a frame-shift, and a shorter isoform (2) with a distinct C-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.263667.1, SRR1660807.17025.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGAGGGCG CCGGCGGCGC GAACGACAAG AAAAAGATAA GTTCTGAACG TCGAAAAGAA 
AAGTCTCGAG ATGCAGCCAG ATCTCGGCGA AGTAAAGAAT CTGAAGTTTT TTATGAGCTT 
GCTCATCAGT TGCCACTTCC ACATAATGTG AGTTCGCATC TTGATAAGGC CTCTGTGATG 
AGGCTTACCA TCAGCTATTT GCGTGTGAGG AAACTTCTGG ATGCTGGTGA TTTGGATATT 
GAAGATGACA TGAAAGCACA GATGAATTGC TTTTATTTGA AAGCCTTGGA TGGTTTTGTT 
ATGGTTCTCA CAGATGATGG TGACATGATT TACATTTCTG ATAATGTGAA CAAATACATG 
GGATTAACTC AGTTTGAACT AACTGGACAC AGTGTGTTTG ATTTTACTCA TCCATGTGAC 
CATGAGGAAA TGAGAGAAAT GCTTACACAC AGAAATGGCC TTGTGAAAAA GGGTAAAGAA 
CAAAACACAC AGCGAAGCTT TTTTCTCAGA ATGAAGTGTA CCCTAACTAG CCGAGGAAGA 
ACTATGAACA TAAAGTCTGC AACATGGAAG GTATTGCACT GCACAGGCCA CATTCACGTA 
TATGATACCA ACAGTAACCA ACCTCAGTGT GGGTATAAGA AACCACCTAT GACCTGCTTG 
GTGCTGATTT GTGAACCCAT TCCTCACCCA TCAAATATTG AAATTCCTTT AGATAGCAAG 
ACTTTCCTCA GTCGACACAG CCTGGATATG AAATTTTCTT ATTGTGATGA AAGAATTACC 
GAATTGATGG GATATGAGCC AGAAGAACTT TTAGGCCGCT CAATTTATGA ATATTATCAT 
GCTTTGGACT CTGATCATCT GACCAAAACT CATCATGATA TGTTTACTAA AGGACAAGTC 
ACCACAGGAC AGTACAGGAT GCTTGCCAAA AGAGGTGGAT ATGTCTGGGT TGAAACTCAA 
GCAACTGTCA TATATAACAC CAAGAATTCT CAACCACAGT GCATTGTATG TGTGAATTAC 
GTTGTGAGTG GTATTATTCA GCACGACTTG ATTTTCTCCC TTCAACAAAC AGAATGTGTC 
CTTAAACCGG TTGAATCTTC AGATATGAAA ATGACTCAGC TATTCACCAA AGTTGAATCA 
GAAGATACAA GTAGCCTCTT TGACAAACTT AAGAAGGAAC CTGATGCTTT AACTTTGCTG 
GCCCCAGCCG CTGGAGACAC AATCATATCT TTAGATTTTG GCAGCAACGA CACAGAAACT 
GATGACCAGC AACTTGAGGA AGTACCATTA TATAATGATG TAATGCTCCC CTCACCCAAC 
GAAAAATTAC AGAATATAAA TTTGGCAATG TCTCCATTAC CCACCGCTGA AACGCCAAAG 
CCACTTCGAA GTAGTGCTGA CCCTGCACTC AATCAAGAAG TTGCATTAAA ATTAGAACCA 
AATCCAGAGT CACTGGAACT TTCTTTTACC ATGCCCCAGA TTCAGGATCA GACACCTAGT 
CCTTCCGATG GAAGCACTAG ACAAAGTTCA CCTGAGCCTA ATAGTCCCAG TGAATATTGT 
TTTTATGTGG ATAGTGATAT GGTCAATGAA TTCAAGTTGG AATTGGTAGA AAAACTTTTT 
GCTGAAGACA CAGAAGCAAA GAACCCATTT TCTACTCAGG ACACAGATTT AGACTTGGAG 
ATGTTAGCTC CCTATATCCC AATGGATGAT GACTTCCAGT TACGTTCCTT CGATCAGTTG 
TCACCATTAG AAAGCAGTTC CGCAAGCCCT GAAAGCGCAA GTCCTCAAAG CACAGTTACA 
GTATTCCAGC AGACTCAAAT ACAAGAACCT ACTGCTAATG CCACCACTAC CACTGCCACC 
ACTGATGAAT TAAAAACAGT GACAAAAGAC CGTATGGAAG ACATTAAAAT ATTGATTGCA 
TCTCCATCTC CTACCCACAT ACATAAAGAA ACTACTAGTG CCACATCATC ACCATATAGA 
GATACTCAAA GTCGGACAGC CTCACCAAAC AGAGCAGGAA AAGGAGTCAT AGAACAGACA 
GAAAAATCTC ATCCAAGAAG CCCTAACGTG TTATCTGTCG CTTTGAGTCA AAGAACTACA 
GTTCCTGAGG AAGAACTAAA TCCAAAGATA CTAGCTTTGC AGAATGCTCA GAGAAAGCGA 
AAAATGGAAC ATGATGGTTC ACTTTTTCAA GCAGTAGGAA TTATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_851397.1
CDS	405..2612
Translation	MEGAGGANDKKKISSERRKEKSRDAARSRRSKESEVFYELAHQLPLPHNVSSHLDKASVMRLTISYLRVRKLLDAGDLDIEDDMKAQMNCFYLKALDGFVMVLTDDGDMIYISDNVNKYMGLTQFELTGHSVFDFTHPCDHEEMREMLTHRNGLVKKGKEQNTQRSFFLRMKCTLTSRGRTMNIKSATWKVLHCTGHIHVYDTNSNQPQCGYKKPPMTCLVLICEPIPHPSNIEIPLDSKTFLSRHSLDMKFSYCDERITELMGYEPEELLGRSIYEYYHALDSDHLTKTHHDMFTKGQVTTGQYRMLAKRGGYVWVETQATVIYNTKNSQPQCIVCVNYVVSGIIQHDLIFSLQQTECVLKPVESSDMKMTQLFTKVESEDTSSLFDKLKKEPDALTLLAPAAGDTIISLDFGSNDTETDDQQLEEVPLYNDVMLPSPNEKLQNINLAMSPLPTAETPKPLRSSADPALNQEVALKLEPNPESLELSFTMPQIQDQTPSPSDGSTRQSSPEPNSPSEYCFYVDSDMVNEFKLELVEKLFAEDTEAKNPFSTQDTDLDLEMLAPYIPMDDDFQLRSFDQLSPLESSSASPESASPQSTVTVFQQTQIQEPTANATTTTATTDELKTVTKDRMEDIKILIASPSPTHIHKETTSATSSPYRDTQSRTASPNRAGKGVIEQTEKSHPRSPNVLSVALSQRTTVPEEELNPKILALQNAQRKRKMEHDGSLFQAVGII

Target ORF information:

RefSeq Version	NM_181054.2
Organism	Homo sapiens(human)
Definition	Homo sapiens hypoxia inducible factor 1 subunit alpha (HIF1A), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_181054.2

ORF Insert Sequence:

ATGGAGGGCG CCGGCGGCGC GAACGACAAG AAAAAGATAA GTTCTGAACG TCGAAAAGAA 
AAGTCTCGAG ATGCAGCCAG ATCTCGGCGA AGTAAAGAAT CTGAAGTTTT TTATGAGCTT 
GCTCATCAGT TGCCACTTCC ACATAATGTG AGTTCGCATC TTGATAAGGC CTCTGTGATG 
AGGCTTACCA TCAGCTATTT GCGTGTGAGG AAACTTCTGG ATGCTGGTGA TTTGGATATT 
GAAGATGACA TGAAAGCACA GATGAATTGC TTTTATTTGA AAGCCTTGGA TGGTTTTGTT 
ATGGTTCTCA CAGATGATGG TGACATGATT TACATTTCTG ATAATGTGAA CAAATACATG 
GGATTAACTC AGTTTGAACT AACTGGACAC AGTGTGTTTG ATTTTACTCA TCCATGTGAC 
CATGAGGAAA TGAGAGAAAT GCTTACACAC AGAAATGGCC TTGTGAAAAA GGGTAAAGAA 
CAAAACACAC AGCGAAGCTT TTTTCTCAGA ATGAAGTGTA CCCTAACTAG CCGAGGAAGA 
ACTATGAACA TAAAGTCTGC AACATGGAAG GTATTGCACT GCACAGGCCA CATTCACGTA 
TATGATACCA ACAGTAACCA ACCTCAGTGT GGGTATAAGA AACCACCTAT GACCTGCTTG 
GTGCTGATTT GTGAACCCAT TCCTCACCCA TCAAATATTG AAATTCCTTT AGATAGCAAG 
ACTTTCCTCA GTCGACACAG CCTGGATATG AAATTTTCTT ATTGTGATGA AAGAATTACC 
GAATTGATGG GATATGAGCC AGAAGAACTT TTAGGCCGCT CAATTTATGA ATATTATCAT 
GCTTTGGACT CTGATCATCT GACCAAAACT CATCATGATA TGTTTACTAA AGGACAAGTC 
ACCACAGGAC AGTACAGGAT GCTTGCCAAA AGAGGTGGAT ATGTCTGGGT TGAAACTCAA 
GCAACTGTCA TATATAACAC CAAGAATTCT CAACCACAGT GCATTGTATG TGTGAATTAC 
GTTGTGAGTG GTATTATTCA GCACGACTTG ATTTTCTCCC TTCAACAAAC AGAATGTGTC 
CTTAAACCGG TTGAATCTTC AGATATGAAA ATGACTCAGC TATTCACCAA AGTTGAATCA 
GAAGATACAA GTAGCCTCTT TGACAAACTT AAGAAGGAAC CTGATGCTTT AACTTTGCTG 
GCCCCAGCCG CTGGAGACAC AATCATATCT TTAGATTTTG GCAGCAACGA CACAGAAACT 
GATGACCAGC AACTTGAGGA AGTACCATTA TATAATGATG TAATGCTCCC CTCACCCAAC 
GAAAAATTAC AGAATATAAA TTTGGCAATG TCTCCATTAC CCACCGCTGA AACGCCAAAG 
CCACTTCGAA GTAGTGCTGA CCCTGCACTC AATCAAGAAG TTGCATTAAA ATTAGAACCA 
AATCCAGAGT CACTGGAACT TTCTTTTACC ATGCCCCAGA TTCAGGATCA GACACCTAGT 
CCTTCCGATG GAAGCACTAG ACAAAGTTCA CCTGAGCCTA ATAGTCCCAG TGAATATTGT 
TTTTATGTGG ATAGTGATAT GGTCAATGAA TTCAAGTTGG AATTGGTAGA AAAACTTTTT 
GCTGAAGACA CAGAAGCAAA GAACCCATTT TCTACTCAGG ACACAGATTT AGACTTGGAG 
ATGTTAGCTC CCTATATCCC AATGGATGAT GACTTCCAGT TACGTTCCTT CGATCAGTTG 
TCACCATTAG AAAGCAGTTC CGCAAGCCCT GAAAGCGCAA GTCCTCAAAG CACAGTTACA 
GTATTCCAGC AGACTCAAAT ACAAGAACCT ACTGCTAATG CCACCACTAC CACTGCCACC 
ACTGATGAAT TAAAAACAGT GACAAAAGAC CGTATGGAAG ACATTAAAAT ATTGATTGCA 
TCTCCATCTC CTACCCACAT ACATAAAGAA ACTACTAGTG CCACATCATC ACCATATAGA 
GATACTCAAA GTCGGACAGC CTCACCAAAC AGAGCAGGAA AAGGAGTCAT AGAACAGACA 
GAAAAATCTC ATCCAAGAAG CCCTAACGTG TTATCTGTCG CTTTGAGTCA AAGAACTACA 
GTTCCTGAGG AAGAACTAAA TCCAAAGATA CTAGCTTTGC AGAATGCTCA GAGAAAGCGA 
AAAATGGAAC ATGATGGTTC ACTTTTTCAA GCAGTAGGAA TTATTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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HIF1A cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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