HIF1A cDNA ORF clone, Bos taurus(cattle)

The following HIF1A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HIF1A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa43417 NM_174339.3
Latest version!
Bos taurus hypoxia inducible factor 1 subunit alpha (HIF1A), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa219407 XM_024997269.1
Latest version!
Bos taurus hypoxia inducible factor 1 subunit alpha (HIF1A), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa219408 XM_024997270.1
Latest version!
Bos taurus hypoxia inducible factor 1 subunit alpha (HIF1A), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa43417
    Clone ID Related Accession (Same CDS sequence) NM_174339.3
    Accession Version NM_174339.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2472bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-23
    Organism Bos taurus(cattle)
    Product hypoxia-inducible factor 1-alpha
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC126622.1. On Nov 13, 2006 this sequence version replaced NM_174339.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC126622.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145414 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAGGGCG CCGGGGGCGC GAACGACAAG AAAAAGATAA GTTCTGAACG TCGAAAAGAG 
    AAGTCTAGAG ATGCAGCCAG ATCTCGTCGA AGTAAAGAGT CTGAAGTTTT TTATGAGCTT
    GCTCATCAGT TGCCACTCCC CCATAATGTA AGCTCGCATC TTGATAAGGC TTCTGTTATG
    AGGCTCACCA TCAGCTATTT GCGTGTGAGG AAACTTCTGG ATGCTGGTGA TTTGGATATT
    GAAGATGAAA TGAAGGCACA GATGAATTGC TTTTATTTGA AAGCCTTGGA TGGTTTTGTT
    ATGGTTCTCA CAGATGATGG TGACATGATT TACATTTCTG ATAATGTGAA CAAATACATG
    GGATTAACTC AGTTTGAACT AACTGGACAC AGTGTGTTTG ATTTTACTCA TCCATGTGAC
    CACGAGGAAA TGAGAGAAAT GCTTACACAC AGAAATGGCC TTGTGAAAAA GGGTAAAGAG
    CAAAATACAC AGCGGAGCTT TTTTCTCAGA ATGAAGTGTA CCCTAACTAG CCGGGGGAGA
    ACTATGAACA TAAAATCTGC AACATGGAAA GTACTTCACT GCACAGGCCA TATTCATGTA
    TACGATACCA ACAGTAACCA ATCTCAGTGT GGGTATAAGA AACCACCGAT GACGTGCTTG
    GTGCTGATTT GTGAACCCAT TCCTCATCCA TCAAATATTG AAATTCCTTT AGACAGCAAG
    ACTTTTCTCA GTCGTCACAG TCTGGATATG AAATTTTCTT ATTGTGATGA AAGAATTACA
    GAATTGATGG GATATGAGCC AGAAGAACTT TTGGGCCGCT CAATTTATGA ATATTATCAT
    GCCTTGGACT CTGACCATCT GACCAAAACT CATCATGATA TGTTTACTAA AGGACAAGTC
    ACAACAGGAC AGTACAGAAT GCTTGCCAAA AGAGGTGGAT ATGTCTGGAT AGAAACTCAA
    GCAACTGTCA TATATAACAC TAAGAACTCT CAGCCCCAGT GCATTGTATG TGTAAATTAT
    GTTGTGAGTG GTATTATTCA GCACGACTTG ATTTTCTCCC TTCAACAAAC AGAATGTGTT
    CTCAAACCAG TTGAATCTTC AGACATGAAA ATGACTCAGC TATTCACCAA AGTTGAATCA
    GAAGATACAA GCAGTCTCTT TGATAAACTT AAGAAGGAGC CTGATGCTTT AACTTTGCTG
    GCCCCAGCTG CTGGAGACAC AATCATATCT TTAGATTTTG GCAGCAATGA CACAGAGACT
    GATGACCAAC AACTTGAGGA AGTTCCATTG TATAATGATG TAATGCTCCC CTCATCTAAT
    GAGAAATTGC AGAATATAAA TTTGGCAATG TCTCCATTAC CTGCCTCTGA AACTCCAAAG
    CCACTTAGAA GTAGTGCTGA CCCTGCACTC AACCAAGAAG TTGCATTAAA GTTAGAACCA
    AATCCAGAGT CACTGGAACT TTCTTTTACC ATGCCCCAGA TTCAAGATCA GCCAGCTAGT
    CCTTCTGATG GAAGCACCAG ACAAAGTTCA CCTGAGCCTA ACAGTCCCAG TGAATATTGT
    TTTGATGTGG ATAGTGATAT GGTCAATGAA TTCAAGTTGG AATTGGTAGA GAAACTTTTT
    GCTGAAGATA CAGAAGCAAA GAATCCATTT TCCACTCAGG ACACTGATTT AGACTTGGAG
    ATGTTAGCTC CTTATATCCC AATGGATGAT GACTTCCAGT TACGTTCCTT CGATCAGTTG
    TCACCACTGG AAAACAGTTC TACAAGTCCT CAGAGTGCAA GCACAAATAC AGTATTCCAG
    CCCACTCAAA TGCAAGAACC TCCTATAGCC ACCGTCACTA CCACAGCCAC CAGTGATGAA
    TTGAAAACAG TGACAAAAGA TGGTATGGAA GACATTAAAA TACTGATTGC ATTTCCATCT
    CCTCCCCACG TACCTAAAGA ACCTCCTTGT GCCACAACGT CACCATATAG TGATACTGGA
    AGTCGGACAG CCTCACCCAA CAGAGCAGGA AAAGGAGTCA TAGAACAGAC AGAAAAATCT
    CATCCAAGAA GCCCTAATGT GTTATCTGTC GCTTTGAGTC AAAGAACTAC TGCTCCTGAG
    GAAGAACTAA ATCCAAAGAT ACTAGCTTTG CAGAATGCTC AGAGAAAGCG AAAAATTGAA
    CATGATGGTT CACTTTTTCA AGCAGTAGGA ATTGGAACAT TATTACAGCA ACCAGATGAT
    CGTGCAACTA CCACATCACT TTCTTGGAAA CGTGTAAAAG GATGCAAATC TAGTGAACAA
    AATGGAATGG AGCAAAAGAC AATTATTTTA ATACCCTCTG ATTTAGCATG TAGACTGCTG
    GGGCAATCAA TGGATGAGAG TGGACTACCA CAGCTGACCA GTTATGATTG TGAAGTTAAT
    GCTCCTATAC AAGGCAGCAG AAACCTACTG CAGGGTGAAG AATTACTCAG AGCTTTGGAT
    CAAGTTAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_776764.2
    CDS280..2751
    Translation

    Target ORF information:

    RefSeq Version NM_174339.3
    Organism Bos taurus(cattle)
    Definition Bos taurus hypoxia inducible factor 1 subunit alpha (HIF1A), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_174339.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGAGGGCG CCGGGGGCGC GAACGACAAG AAAAAGATAA GTTCTGAACG TCGAAAAGAG 
    AAGTCTAGAG ATGCAGCCAG ATCTCGTCGA AGTAAAGAGT CTGAAGTTTT TTATGAGCTT
    GCTCATCAGT TGCCACTCCC CCATAATGTA AGCTCGCATC TTGATAAGGC TTCTGTTATG
    AGGCTCACCA TCAGCTATTT GCGTGTGAGG AAACTTCTGG ATGCTGGTGA TTTGGATATT
    GAAGATGAAA TGAAGGCACA GATGAATTGC TTTTATTTGA AAGCCTTGGA TGGTTTTGTT
    ATGGTTCTCA CAGATGATGG TGACATGATT TACATTTCTG ATAATGTGAA CAAATACATG
    GGATTAACTC AGTTTGAACT AACTGGACAC AGTGTGTTTG ATTTTACTCA TCCATGTGAC
    CACGAGGAAA TGAGAGAAAT GCTTACACAC AGAAATGGCC TTGTGAAAAA GGGTAAAGAG
    CAAAATACAC AGCGGAGCTT TTTTCTCAGA ATGAAGTGTA CCCTAACTAG CCGGGGGAGA
    ACTATGAACA TAAAATCTGC AACATGGAAA GTACTTCACT GCACAGGCCA TATTCATGTA
    TACGATACCA ACAGTAACCA ATCTCAGTGT GGGTATAAGA AACCACCGAT GACGTGCTTG
    GTGCTGATTT GTGAACCCAT TCCTCATCCA TCAAATATTG AAATTCCTTT AGACAGCAAG
    ACTTTTCTCA GTCGTCACAG TCTGGATATG AAATTTTCTT ATTGTGATGA AAGAATTACA
    GAATTGATGG GATATGAGCC AGAAGAACTT TTGGGCCGCT CAATTTATGA ATATTATCAT
    GCCTTGGACT CTGACCATCT GACCAAAACT CATCATGATA TGTTTACTAA AGGACAAGTC
    ACAACAGGAC AGTACAGAAT GCTTGCCAAA AGAGGTGGAT ATGTCTGGAT AGAAACTCAA
    GCAACTGTCA TATATAACAC TAAGAACTCT CAGCCCCAGT GCATTGTATG TGTAAATTAT
    GTTGTGAGTG GTATTATTCA GCACGACTTG ATTTTCTCCC TTCAACAAAC AGAATGTGTT
    CTCAAACCAG TTGAATCTTC AGACATGAAA ATGACTCAGC TATTCACCAA AGTTGAATCA
    GAAGATACAA GCAGTCTCTT TGATAAACTT AAGAAGGAGC CTGATGCTTT AACTTTGCTG
    GCCCCAGCTG CTGGAGACAC AATCATATCT TTAGATTTTG GCAGCAATGA CACAGAGACT
    GATGACCAAC AACTTGAGGA AGTTCCATTG TATAATGATG TAATGCTCCC CTCATCTAAT
    GAGAAATTGC AGAATATAAA TTTGGCAATG TCTCCATTAC CTGCCTCTGA AACTCCAAAG
    CCACTTAGAA GTAGTGCTGA CCCTGCACTC AACCAAGAAG TTGCATTAAA GTTAGAACCA
    AATCCAGAGT CACTGGAACT TTCTTTTACC ATGCCCCAGA TTCAAGATCA GCCAGCTAGT
    CCTTCTGATG GAAGCACCAG ACAAAGTTCA CCTGAGCCTA ACAGTCCCAG TGAATATTGT
    TTTGATGTGG ATAGTGATAT GGTCAATGAA TTCAAGTTGG AATTGGTAGA GAAACTTTTT
    GCTGAAGATA CAGAAGCAAA GAATCCATTT TCCACTCAGG ACACTGATTT AGACTTGGAG
    ATGTTAGCTC CTTATATCCC AATGGATGAT GACTTCCAGT TACGTTCCTT CGATCAGTTG
    TCACCACTGG AAAACAGTTC TACAAGTCCT CAGAGTGCAA GCACAAATAC AGTATTCCAG
    CCCACTCAAA TGCAAGAACC TCCTATAGCC ACCGTCACTA CCACAGCCAC CAGTGATGAA
    TTGAAAACAG TGACAAAAGA TGGTATGGAA GACATTAAAA TACTGATTGC ATTTCCATCT
    CCTCCCCACG TACCTAAAGA ACCTCCTTGT GCCACAACGT CACCATATAG TGATACTGGA
    AGTCGGACAG CCTCACCCAA CAGAGCAGGA AAAGGAGTCA TAGAACAGAC AGAAAAATCT
    CATCCAAGAA GCCCTAATGT GTTATCTGTC GCTTTGAGTC AAAGAACTAC TGCTCCTGAG
    GAAGAACTAA ATCCAAAGAT ACTAGCTTTG CAGAATGCTC AGAGAAAGCG AAAAATTGAA
    CATGATGGTT CACTTTTTCA AGCAGTAGGA ATTGGAACAT TATTACAGCA ACCAGATGAT
    CGTGCAACTA CCACATCACT TTCTTGGAAA CGTGTAAAAG GATGCAAATC TAGTGAACAA
    AATGGAATGG AGCAAAAGAC AATTATTTTA ATACCCTCTG ATTTAGCATG TAGACTGCTG
    GGGCAATCAA TGGATGAGAG TGGACTACCA CAGCTGACCA GTTATGATTG TGAAGTTAAT
    GCTCCTATAC AAGGCAGCAG AAACCTACTG CAGGGTGAAG AATTACTCAG AGCTTTGGAT
    CAAGTTAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa219407
    Clone ID Related Accession (Same CDS sequence) XM_024997269.1
    Accession Version XM_024997269.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2481bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product hypoxia-inducible factor 1-alpha isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGCTGGGAA GATCCTTAAC ATACTGCTCA GTGCTTAGTA AATGGATAAG TTCTGAACGT 
    CGAAAAGAGA AGTCTAGAGA TGCAGCCAGA TCTCGTCGAA GTAAAGAGTC TGAAGTTTTT
    TATGAGCTTG CTCATCAGTT GCCACTCCCC CATAATGTAA GCTCGCATCT TGATAAGGCT
    TCTGTTATGA GGCTCACCAT CAGCTATTTG CGTGTGAGGA AACTTCTGGA TGCTGGTGAT
    TTGGATATTG AAGATGAAAT GAAGGCACAG ATGAATTGCT TTTATTTGAA AGCCTTGGAT
    GGTTTTGTTA TGGTTCTCAC AGATGATGGT GACATGATTT ACATTTCTGA TAATGTGAAC
    AAATACATGG GATTAACTCA GTTTGAACTA ACTGGACACA GTGTGTTTGA TTTTACTCAT
    CCATGTGACC ACGAGGAAAT GAGAGAAATG CTTACACACA GAAATGGCCT TGTGAAAAAG
    GGTAAAGAGC AAAATACACA GCGGAGCTTT TTTCTCAGAA TGAAGTGTAC CCTAACTAGC
    CGGGGGAGAA CTATGAACAT AAAATCTGCA ACATGGAAAG TACTTCACTG CACAGGCCAT
    ATTCATGTAT ACGATACCAA CAGTAACCAA TCTCAGTGTG GGTATAAGAA ACCACCGATG
    ACGTGCTTGG TGCTGATTTG TGAACCCATT CCTCATCCAT CAAATATTGA AATTCCTTTA
    GACAGCAAGA CTTTTCTCAG TCGTCACAGT CTGGATATGA AATTTTCTTA TTGTGATGAA
    AGAATTACAG AATTGATGGG ATATGAGCCA GAAGAACTTT TGGGCCGCTC AATTTATGAA
    TATTATCATG CCTTGGACTC TGACCATCTG ACCAAAACTC ATCATGATAT GTTTACTAAA
    GGACAAGTCA CAACAGGACA GTACAGAATG CTTGCCAAAA GAGGTGGATA TGTCTGGATA
    GAAACTCAAG CAACTGTCAT ATATAACACT AAGAACTCTC AGCCCCAGTG CATTGTATGT
    GTAAATTATG TTGTGAGTGG TATTATTCAG CACGACTTGA TTTTCTCCCT TCAACAAACA
    GAATGTGTTC TCAAACCAGT TGAATCTTCA GACATGAAAA TGACTCAGCT ATTCACCAAA
    GTTGAATCAG AAGATACAAG CAGTCTCTTT GATAAACTTA AGAAGGAGCC TGATGCTTTA
    ACTTTGCTGG CCCCAGCTGC TGGAGACACA ATCATATCTT TAGATTTTGG CAGCAATGAC
    ACAGAGACTG ATGACCAACA ACTTGAGGAA GTTCCATTGT ATAATGATGT AATGCTCCCC
    TCATCTAATG AGAAATTGCA GAATATAAAT TTGGCAATGT CTCCATTACC TGCCTCTGAA
    ACTCCAAAGC CACTTAGAAG TAGTGCTGAC CCTGCACTCA ACCAAGAAGT TGCATTAAAG
    TTAGAACCAA ATCCAGAGTC ACTGGAACTT TCTTTTACCA TGCCCCAGAT TCAAGATCAG
    CCAGCTAGTC CTTCTGATGG AAGCACCAGA CAAAGTTCAC CTGAGCCTAA CAGTCCCAGT
    GAATATTGTT TTGATGTGGA TAGTGATATG GTCAATGAAT TCAAGTTGGA ATTGGTAGAG
    AAACTTTTTG CTGAAGATAC AGAAGCAAAG AATCCATTTT CCACTCAGGA CACTGATTTA
    GACTTGGAGA TGTTAGCTCC TTATATCCCA ATGGATGATG ACTTCCAGTT ACGTTCCTTC
    GATCAGTTGT CACCACTGGA AAACAGTTCT ACAAGTCCTC AGAGTGCAAG CACAAATACA
    GTATTCCAGC CCACTCAAAT GCAAGAACCT CCTATAGCCA CCGTCACTAC CACAGCCACC
    AGTGATGAAT TGAAAACAGT GACAAAAGAT GGTATGGAAG ACATTAAAAT ACTGATTGCA
    TTTCCATCTC CTCCCCACGT ACCTAAAGAA CCTCCTTGTG CCACAACGTC ACCATATAGT
    GATACTGGAA GTCGGACAGC CTCACCCAAC AGAGCAGGAA AAGGAGTCAT AGAACAGACA
    GAAAAATCTC ATCCAAGAAG CCCTAATGTG TTATCTGTCG CTTTGAGTCA AAGAACTACT
    GCTCCTGAGG AAGAACTAAA TCCAAAGATA CTAGCTTTGC AGAATGCTCA GAGAAAGCGA
    AAAATTGAAC ATGATGGTTC ACTTTTTCAA GCAGTAGGAA TTGGAACATT ATTACAGCAA
    CCAGATGATC GTGCAACTAC CACATCACTT TCTTGGAAAC GTGTAAAAGG ATGCAAATCT
    AGTGAACAAA ATGGAATGGA GCAAAAGACA ATTATTTTAA TACCCTCTGA TTTAGCATGT
    AGACTGCTGG GGCAATCAAT GGATGAGAGT GGACTACCAC AGCTGACCAG TTATGATTGT
    GAAGTTAATG CTCCTATACA AGGCAGCAGA AACCTACTGC AGGGTGAAGA ATTACTCAGA
    GCTTTGGATC AAGTTAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024853037.1
    CDS1125..3605
    Translation

    Target ORF information:

    RefSeq Version XM_024997269.1
    Organism Bos taurus(cattle)
    Definition Bos taurus hypoxia inducible factor 1 subunit alpha (HIF1A), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024997269.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGCTGGGAA GATCCTTAAC ATACTGCTCA GTGCTTAGTA AATGGATAAG TTCTGAACGT 
    CGAAAAGAGA AGTCTAGAGA TGCAGCCAGA TCTCGTCGAA GTAAAGAGTC TGAAGTTTTT
    TATGAGCTTG CTCATCAGTT GCCACTCCCC CATAATGTAA GCTCGCATCT TGATAAGGCT
    TCTGTTATGA GGCTCACCAT CAGCTATTTG CGTGTGAGGA AACTTCTGGA TGCTGGTGAT
    TTGGATATTG AAGATGAAAT GAAGGCACAG ATGAATTGCT TTTATTTGAA AGCCTTGGAT
    GGTTTTGTTA TGGTTCTCAC AGATGATGGT GACATGATTT ACATTTCTGA TAATGTGAAC
    AAATACATGG GATTAACTCA GTTTGAACTA ACTGGACACA GTGTGTTTGA TTTTACTCAT
    CCATGTGACC ACGAGGAAAT GAGAGAAATG CTTACACACA GAAATGGCCT TGTGAAAAAG
    GGTAAAGAGC AAAATACACA GCGGAGCTTT TTTCTCAGAA TGAAGTGTAC CCTAACTAGC
    CGGGGGAGAA CTATGAACAT AAAATCTGCA ACATGGAAAG TACTTCACTG CACAGGCCAT
    ATTCATGTAT ACGATACCAA CAGTAACCAA TCTCAGTGTG GGTATAAGAA ACCACCGATG
    ACGTGCTTGG TGCTGATTTG TGAACCCATT CCTCATCCAT CAAATATTGA AATTCCTTTA
    GACAGCAAGA CTTTTCTCAG TCGTCACAGT CTGGATATGA AATTTTCTTA TTGTGATGAA
    AGAATTACAG AATTGATGGG ATATGAGCCA GAAGAACTTT TGGGCCGCTC AATTTATGAA
    TATTATCATG CCTTGGACTC TGACCATCTG ACCAAAACTC ATCATGATAT GTTTACTAAA
    GGACAAGTCA CAACAGGACA GTACAGAATG CTTGCCAAAA GAGGTGGATA TGTCTGGATA
    GAAACTCAAG CAACTGTCAT ATATAACACT AAGAACTCTC AGCCCCAGTG CATTGTATGT
    GTAAATTATG TTGTGAGTGG TATTATTCAG CACGACTTGA TTTTCTCCCT TCAACAAACA
    GAATGTGTTC TCAAACCAGT TGAATCTTCA GACATGAAAA TGACTCAGCT ATTCACCAAA
    GTTGAATCAG AAGATACAAG CAGTCTCTTT GATAAACTTA AGAAGGAGCC TGATGCTTTA
    ACTTTGCTGG CCCCAGCTGC TGGAGACACA ATCATATCTT TAGATTTTGG CAGCAATGAC
    ACAGAGACTG ATGACCAACA ACTTGAGGAA GTTCCATTGT ATAATGATGT AATGCTCCCC
    TCATCTAATG AGAAATTGCA GAATATAAAT TTGGCAATGT CTCCATTACC TGCCTCTGAA
    ACTCCAAAGC CACTTAGAAG TAGTGCTGAC CCTGCACTCA ACCAAGAAGT TGCATTAAAG
    TTAGAACCAA ATCCAGAGTC ACTGGAACTT TCTTTTACCA TGCCCCAGAT TCAAGATCAG
    CCAGCTAGTC CTTCTGATGG AAGCACCAGA CAAAGTTCAC CTGAGCCTAA CAGTCCCAGT
    GAATATTGTT TTGATGTGGA TAGTGATATG GTCAATGAAT TCAAGTTGGA ATTGGTAGAG
    AAACTTTTTG CTGAAGATAC AGAAGCAAAG AATCCATTTT CCACTCAGGA CACTGATTTA
    GACTTGGAGA TGTTAGCTCC TTATATCCCA ATGGATGATG ACTTCCAGTT ACGTTCCTTC
    GATCAGTTGT CACCACTGGA AAACAGTTCT ACAAGTCCTC AGAGTGCAAG CACAAATACA
    GTATTCCAGC CCACTCAAAT GCAAGAACCT CCTATAGCCA CCGTCACTAC CACAGCCACC
    AGTGATGAAT TGAAAACAGT GACAAAAGAT GGTATGGAAG ACATTAAAAT ACTGATTGCA
    TTTCCATCTC CTCCCCACGT ACCTAAAGAA CCTCCTTGTG CCACAACGTC ACCATATAGT
    GATACTGGAA GTCGGACAGC CTCACCCAAC AGAGCAGGAA AAGGAGTCAT AGAACAGACA
    GAAAAATCTC ATCCAAGAAG CCCTAATGTG TTATCTGTCG CTTTGAGTCA AAGAACTACT
    GCTCCTGAGG AAGAACTAAA TCCAAAGATA CTAGCTTTGC AGAATGCTCA GAGAAAGCGA
    AAAATTGAAC ATGATGGTTC ACTTTTTCAA GCAGTAGGAA TTGGAACATT ATTACAGCAA
    CCAGATGATC GTGCAACTAC CACATCACTT TCTTGGAAAC GTGTAAAAGG ATGCAAATCT
    AGTGAACAAA ATGGAATGGA GCAAAAGACA ATTATTTTAA TACCCTCTGA TTTAGCATGT
    AGACTGCTGG GGCAATCAAT GGATGAGAGT GGACTACCAC AGCTGACCAG TTATGATTGT
    GAAGTTAATG CTCCTATACA AGGCAGCAGA AACCTACTGC AGGGTGAAGA ATTACTCAGA
    GCTTTGGATC AAGTTAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa219408
    Clone ID Related Accession (Same CDS sequence) XM_024997270.1
    Accession Version XM_024997270.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2475bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product hypoxia-inducible factor 1-alpha isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGCCTGGG ATGCTAACTT TTTCGTTTGC ACGTGCAGGA TAAGTTCTGA ACGTCGAAAA 
    GAGAAGTCTA GAGATGCAGC CAGATCTCGT CGAAGTAAAG AGTCTGAAGT TTTTTATGAG
    CTTGCTCATC AGTTGCCACT CCCCCATAAT GTAAGCTCGC ATCTTGATAA GGCTTCTGTT
    ATGAGGCTCA CCATCAGCTA TTTGCGTGTG AGGAAACTTC TGGATGCTGG TGATTTGGAT
    ATTGAAGATG AAATGAAGGC ACAGATGAAT TGCTTTTATT TGAAAGCCTT GGATGGTTTT
    GTTATGGTTC TCACAGATGA TGGTGACATG ATTTACATTT CTGATAATGT GAACAAATAC
    ATGGGATTAA CTCAGTTTGA ACTAACTGGA CACAGTGTGT TTGATTTTAC TCATCCATGT
    GACCACGAGG AAATGAGAGA AATGCTTACA CACAGAAATG GCCTTGTGAA AAAGGGTAAA
    GAGCAAAATA CACAGCGGAG CTTTTTTCTC AGAATGAAGT GTACCCTAAC TAGCCGGGGG
    AGAACTATGA ACATAAAATC TGCAACATGG AAAGTACTTC ACTGCACAGG CCATATTCAT
    GTATACGATA CCAACAGTAA CCAATCTCAG TGTGGGTATA AGAAACCACC GATGACGTGC
    TTGGTGCTGA TTTGTGAACC CATTCCTCAT CCATCAAATA TTGAAATTCC TTTAGACAGC
    AAGACTTTTC TCAGTCGTCA CAGTCTGGAT ATGAAATTTT CTTATTGTGA TGAAAGAATT
    ACAGAATTGA TGGGATATGA GCCAGAAGAA CTTTTGGGCC GCTCAATTTA TGAATATTAT
    CATGCCTTGG ACTCTGACCA TCTGACCAAA ACTCATCATG ATATGTTTAC TAAAGGACAA
    GTCACAACAG GACAGTACAG AATGCTTGCC AAAAGAGGTG GATATGTCTG GATAGAAACT
    CAAGCAACTG TCATATATAA CACTAAGAAC TCTCAGCCCC AGTGCATTGT ATGTGTAAAT
    TATGTTGTGA GTGGTATTAT TCAGCACGAC TTGATTTTCT CCCTTCAACA AACAGAATGT
    GTTCTCAAAC CAGTTGAATC TTCAGACATG AAAATGACTC AGCTATTCAC CAAAGTTGAA
    TCAGAAGATA CAAGCAGTCT CTTTGATAAA CTTAAGAAGG AGCCTGATGC TTTAACTTTG
    CTGGCCCCAG CTGCTGGAGA CACAATCATA TCTTTAGATT TTGGCAGCAA TGACACAGAG
    ACTGATGACC AACAACTTGA GGAAGTTCCA TTGTATAATG ATGTAATGCT CCCCTCATCT
    AATGAGAAAT TGCAGAATAT AAATTTGGCA ATGTCTCCAT TACCTGCCTC TGAAACTCCA
    AAGCCACTTA GAAGTAGTGC TGACCCTGCA CTCAACCAAG AAGTTGCATT AAAGTTAGAA
    CCAAATCCAG AGTCACTGGA ACTTTCTTTT ACCATGCCCC AGATTCAAGA TCAGCCAGCT
    AGTCCTTCTG ATGGAAGCAC CAGACAAAGT TCACCTGAGC CTAACAGTCC CAGTGAATAT
    TGTTTTGATG TGGATAGTGA TATGGTCAAT GAATTCAAGT TGGAATTGGT AGAGAAACTT
    TTTGCTGAAG ATACAGAAGC AAAGAATCCA TTTTCCACTC AGGACACTGA TTTAGACTTG
    GAGATGTTAG CTCCTTATAT CCCAATGGAT GATGACTTCC AGTTACGTTC CTTCGATCAG
    TTGTCACCAC TGGAAAACAG TTCTACAAGT CCTCAGAGTG CAAGCACAAA TACAGTATTC
    CAGCCCACTC AAATGCAAGA ACCTCCTATA GCCACCGTCA CTACCACAGC CACCAGTGAT
    GAATTGAAAA CAGTGACAAA AGATGGTATG GAAGACATTA AAATACTGAT TGCATTTCCA
    TCTCCTCCCC ACGTACCTAA AGAACCTCCT TGTGCCACAA CGTCACCATA TAGTGATACT
    GGAAGTCGGA CAGCCTCACC CAACAGAGCA GGAAAAGGAG TCATAGAACA GACAGAAAAA
    TCTCATCCAA GAAGCCCTAA TGTGTTATCT GTCGCTTTGA GTCAAAGAAC TACTGCTCCT
    GAGGAAGAAC TAAATCCAAA GATACTAGCT TTGCAGAATG CTCAGAGAAA GCGAAAAATT
    GAACATGATG GTTCACTTTT TCAAGCAGTA GGAATTGGAA CATTATTACA GCAACCAGAT
    GATCGTGCAA CTACCACATC ACTTTCTTGG AAACGTGTAA AAGGATGCAA ATCTAGTGAA
    CAAAATGGAA TGGAGCAAAA GACAATTATT TTAATACCCT CTGATTTAGC ATGTAGACTG
    CTGGGGCAAT CAATGGATGA GAGTGGACTA CCACAGCTGA CCAGTTATGA TTGTGAAGTT
    AATGCTCCTA TACAAGGCAG CAGAAACCTA CTGCAGGGTG AAGAATTACT CAGAGCTTTG
    GATCAAGTTA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024853038.1
    CDS65..2539
    Translation

    Target ORF information:

    RefSeq Version XM_024997270.1
    Organism Bos taurus(cattle)
    Definition Bos taurus hypoxia inducible factor 1 subunit alpha (HIF1A), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024997270.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGCCTGGG ATGCTAACTT TTTCGTTTGC ACGTGCAGGA TAAGTTCTGA ACGTCGAAAA 
    GAGAAGTCTA GAGATGCAGC CAGATCTCGT CGAAGTAAAG AGTCTGAAGT TTTTTATGAG
    CTTGCTCATC AGTTGCCACT CCCCCATAAT GTAAGCTCGC ATCTTGATAA GGCTTCTGTT
    ATGAGGCTCA CCATCAGCTA TTTGCGTGTG AGGAAACTTC TGGATGCTGG TGATTTGGAT
    ATTGAAGATG AAATGAAGGC ACAGATGAAT TGCTTTTATT TGAAAGCCTT GGATGGTTTT
    GTTATGGTTC TCACAGATGA TGGTGACATG ATTTACATTT CTGATAATGT GAACAAATAC
    ATGGGATTAA CTCAGTTTGA ACTAACTGGA CACAGTGTGT TTGATTTTAC TCATCCATGT
    GACCACGAGG AAATGAGAGA AATGCTTACA CACAGAAATG GCCTTGTGAA AAAGGGTAAA
    GAGCAAAATA CACAGCGGAG CTTTTTTCTC AGAATGAAGT GTACCCTAAC TAGCCGGGGG
    AGAACTATGA ACATAAAATC TGCAACATGG AAAGTACTTC ACTGCACAGG CCATATTCAT
    GTATACGATA CCAACAGTAA CCAATCTCAG TGTGGGTATA AGAAACCACC GATGACGTGC
    TTGGTGCTGA TTTGTGAACC CATTCCTCAT CCATCAAATA TTGAAATTCC TTTAGACAGC
    AAGACTTTTC TCAGTCGTCA CAGTCTGGAT ATGAAATTTT CTTATTGTGA TGAAAGAATT
    ACAGAATTGA TGGGATATGA GCCAGAAGAA CTTTTGGGCC GCTCAATTTA TGAATATTAT
    CATGCCTTGG ACTCTGACCA TCTGACCAAA ACTCATCATG ATATGTTTAC TAAAGGACAA
    GTCACAACAG GACAGTACAG AATGCTTGCC AAAAGAGGTG GATATGTCTG GATAGAAACT
    CAAGCAACTG TCATATATAA CACTAAGAAC TCTCAGCCCC AGTGCATTGT ATGTGTAAAT
    TATGTTGTGA GTGGTATTAT TCAGCACGAC TTGATTTTCT CCCTTCAACA AACAGAATGT
    GTTCTCAAAC CAGTTGAATC TTCAGACATG AAAATGACTC AGCTATTCAC CAAAGTTGAA
    TCAGAAGATA CAAGCAGTCT CTTTGATAAA CTTAAGAAGG AGCCTGATGC TTTAACTTTG
    CTGGCCCCAG CTGCTGGAGA CACAATCATA TCTTTAGATT TTGGCAGCAA TGACACAGAG
    ACTGATGACC AACAACTTGA GGAAGTTCCA TTGTATAATG ATGTAATGCT CCCCTCATCT
    AATGAGAAAT TGCAGAATAT AAATTTGGCA ATGTCTCCAT TACCTGCCTC TGAAACTCCA
    AAGCCACTTA GAAGTAGTGC TGACCCTGCA CTCAACCAAG AAGTTGCATT AAAGTTAGAA
    CCAAATCCAG AGTCACTGGA ACTTTCTTTT ACCATGCCCC AGATTCAAGA TCAGCCAGCT
    AGTCCTTCTG ATGGAAGCAC CAGACAAAGT TCACCTGAGC CTAACAGTCC CAGTGAATAT
    TGTTTTGATG TGGATAGTGA TATGGTCAAT GAATTCAAGT TGGAATTGGT AGAGAAACTT
    TTTGCTGAAG ATACAGAAGC AAAGAATCCA TTTTCCACTC AGGACACTGA TTTAGACTTG
    GAGATGTTAG CTCCTTATAT CCCAATGGAT GATGACTTCC AGTTACGTTC CTTCGATCAG
    TTGTCACCAC TGGAAAACAG TTCTACAAGT CCTCAGAGTG CAAGCACAAA TACAGTATTC
    CAGCCCACTC AAATGCAAGA ACCTCCTATA GCCACCGTCA CTACCACAGC CACCAGTGAT
    GAATTGAAAA CAGTGACAAA AGATGGTATG GAAGACATTA AAATACTGAT TGCATTTCCA
    TCTCCTCCCC ACGTACCTAA AGAACCTCCT TGTGCCACAA CGTCACCATA TAGTGATACT
    GGAAGTCGGA CAGCCTCACC CAACAGAGCA GGAAAAGGAG TCATAGAACA GACAGAAAAA
    TCTCATCCAA GAAGCCCTAA TGTGTTATCT GTCGCTTTGA GTCAAAGAAC TACTGCTCCT
    GAGGAAGAAC TAAATCCAAA GATACTAGCT TTGCAGAATG CTCAGAGAAA GCGAAAAATT
    GAACATGATG GTTCACTTTT TCAAGCAGTA GGAATTGGAA CATTATTACA GCAACCAGAT
    GATCGTGCAA CTACCACATC ACTTTCTTGG AAACGTGTAA AAGGATGCAA ATCTAGTGAA
    CAAAATGGAA TGGAGCAAAA GACAATTATT TTAATACCCT CTGATTTAGC ATGTAGACTG
    CTGGGGCAAT CAATGGATGA GAGTGGACTA CCACAGCTGA CCAGTTATGA TTGTGAAGTT
    AATGCTCCTA TACAAGGCAG CAGAAACCTA CTGCAGGGTG AAGAATTACT CAGAGCTTTG
    GATCAAGTTA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.