gRNAs
The following HIF1A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HIF1A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | OBa43417 | |
| Clone ID Related Accession (Same CDS sequence) | NM_174339.3 | |
| Accession Version | NM_174339.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2472bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-12-23 | |
| Organism | Bos taurus(cattle) | |
| Product | hypoxia-inducible factor 1-alpha | |
| Comment | Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC126622.1. On Nov 13, 2006 this sequence version replaced NM_174339.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC126622.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145414 [ECO:0000348] ##Evidence-Data-END## | |
1 | ATGGAGGGCG CCGGGGGCGC GAACGACAAG AAAAAGATAA GTTCTGAACG TCGAAAAGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_776764.2 |
| CDS | 280..2751 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_174339.3 |
1 | ATGGAGGGCG CCGGGGGCGC GAACGACAAG AAAAAGATAA GTTCTGAACG TCGAAAAGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OBa219407 | |
| Clone ID Related Accession (Same CDS sequence) | XM_024997269.1 | |
| Accession Version | XM_024997269.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2481bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2018-05-06 | |
| Organism | Bos taurus(cattle) | |
| Product | hypoxia-inducible factor 1-alpha isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGCTGGGAA GATCCTTAAC ATACTGCTCA GTGCTTAGTA AATGGATAAG TTCTGAACGT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_024853037.1 |
| CDS | 1125..3605 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_024997269.1 |
1 | ATGCTGGGAA GATCCTTAAC ATACTGCTCA GTGCTTAGTA AATGGATAAG TTCTGAACGT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | OBa219408 | |
| Clone ID Related Accession (Same CDS sequence) | XM_024997270.1 | |
| Accession Version | XM_024997270.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2475bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2018-05-06 | |
| Organism | Bos taurus(cattle) | |
| Product | hypoxia-inducible factor 1-alpha isoform X2 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGCCTGGG ATGCTAACTT TTTCGTTTGC ACGTGCAGGA TAAGTTCTGA ACGTCGAAAA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_024853038.1 |
| CDS | 65..2539 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_024997270.1 |
1 | ATGGCCTGGG ATGCTAACTT TTTCGTTTGC ACGTGCAGGA TAAGTTCTGA ACGTCGAAAA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Expression pattern of HIF1alpha and vasohibins during follicle maturation and corpus luteum function in the bovine ovary. |
Glucose transport and milk secretion during manipulated plasma insulin and glucose concentrations and during LPS-induced mastitis in dairy cows. |
Glucose transporter and hypoxia-associated gene expression in the mammary gland of transition dairy cattle. |
Hypoxia is important for establishing vascularization during corpus luteum formation in cattle. |
Sphingosine-1-phosphate: a novel nonhypoxic activator of hypoxia-inducible factor-1 in vascular cells. |
