Bcas2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Bcas2
Entrez Gene ID	295334
Full Name	BCAS2, pre-mRNA processing factor
General protein information	Preferred Names BCAS2, pre-mRNA processing factor Names pre-mRNA-splicing factor SPF27 breast carcinoma amplified sequence 2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q34

mRNA and Protein(s)

mRNA	Protein	Name
NM_001106458.2	NP_001099928.1	pre-mRNA-splicing factor SPF27

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Bcas2	NP_001099928.1
Gallus gallus (chicken)	BCAS2	NP_001244224.1
Danio rerio (zebrafish)	bcas2	NP_001007775.1
Arabidopsis thaliana (thale cress)	MOS4	NP_566599.1
Homo sapiens (human)	BCAS2	NP_005863.1
Drosophila melanogaster (fruit fly)	CG4980	NP_651596.1
Xenopus tropicalis (tropical clawed frog)	bcas2	NP_988924.1
Canis lupus familiaris (dog)	BCAS2	XP_533014.1
Bos taurus (cattle)	BCAS2	NP_001015540.1
Mus musculus (house mouse)	Bcas2	NP_080878.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002305	XP_312666.3
Pan troglodytes (chimpanzee)	BCAS2	XP_003308351.1
Caenorhabditis elegans (roundworm)	T12A2.7	NP_498360.2

Gene Ontology
Bibliography

Related articles in PubMed

Cryo-EM structure of a human spliceosome activated for step 2 of splicing.
Bertram K, Agafonov DE, Liu WT, Dybkov O, Will CL, Hartmuth K, Urlaub H, Kastner B, Stark H, L?hrmann R
Nature542(7641)318-323(2017 02)

PRP19 transforms into a sensor of RPA-ssDNA after DNA damage and drives ATR activation via a ubiquitin-mediated circuitry.
Mar?chal A, Li JM, Ji XY, Wu CS, Yazinski SA, Nguyen HD, Liu S, Jim?nez AE, Jin J, Zou L
Molecular cell53(2)235-246(2014 Jan)

Splicing and beyond: the many faces of the Prp19 complex.
Chanarat S, Str??er K
Biochimica et biophysica acta1833(10)2126-34(2013 Oct)

Amplification of the BCAS2 gene at chromosome 1p13.3-21 in human primary breast cancer.
Maass N, R?sel F, Schem C, Hitomi J, Jonat W, Nagasaki K
Cancer letters185(2)219-23(2002 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Bcas2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Bcas2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa01338	NM_001106458.2 Latest version!	Rattus norvegicus BCAS2, pre-mRNA processing factor (Bcas2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Bcas2 ( NM_001106458.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001099928.1 Rattus norvegicus BCAS2, pre-mRNA processing factor (Bcas2), mRNA.

Price & Availability↑

CloneID	ORa01338
Clone ID Related Accession (Same CDS sequence)	NM_001106458.2
Accession Version	NM_001106458.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 678bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-27
Organism	Rattus norvegicus(Norway rat)
Product	pre-mRNA-splicing factor SPF27
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC169122.1. On Sep 4, 2008 this sequence version replaced NM_001106458.1. ##Evidence-Data-START## Transcript exon combination :: BC169122.1, FQ226799.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGGCA CGGGCTTGGT AGCGGGGGAG GTGGTAGTGG ATGCGCTACC CTACTTTGAC 
CAAGGCTATG AAGCGCCTGG CGTGCGGGAA GCGGCTGCGG CCCTGGTGGA GGAGGAAACG 
CGCAGATACC GACCTACCAA GAACTACCTG AGCTACCTGA CAGCCCCAGA TTATTCTGCC 
TTTGAAACAG ACATAATGAG AAATGAGTTT GAAAGACTGG CTGCTCGACA ACCAATTGAA 
TTACTCAGCA TGAAGCGATA TGAACTGCCA GCCCCTTCCT CGGGTCAAAA AAATGACATC 
ACTGCGTGGC AAGACTGTGT AAACAATTCT ATGGCCCAGC TGGAGCATCA GGCAGTGCGG 
ATTGAGAATC TGGAACTGAT GTCACAGCAT GGGTGTAACG CCTGGAAAGT GTACAACGAA 
AACCTTGTTC ACATGATTGA ACATGCACAG AAAGAGCTTC AGAAGTTAAG GAAGCATATT 
CAGGATTTAA ACTGGCAGCG CAAGAACATG CAGCTTACAG CTGGGGCTAA GCTGAGAGAA 
ATGGAGTCAA ATTGGGTGTC GCTGGTGAGT AAGAACTATG AGATTGAGCG GACTATTGTA 
CAGTTGGAGA ACGAGATCTA TCAAATCAAG CAGCAGCACG GTGAGGCAAA CAAGGAAAAC 
ATCCGCCAAG ACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001099928.1
CDS	18..695
Translation	MAGTGLVAGEVVVDALPYFDQGYEAPGVREAAAALVEEETRRYRPTKNYLSYLTAPDYSAFETDIMRNEFERLAARQPIELLSMKRYELPAPSSGQKNDITAWQDCVNNSMAQLEHQAVRIENLELMSQHGCNAWKVYNENLVHMIEHAQKELQKLRKHIQDLNWQRKNMQLTAGAKLREMESNWVSLVSKNYEIERTIVQLENEIYQIKQQHGEANKENIRQDF

Target ORF information:

RefSeq Version	NM_001106458.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus BCAS2, pre-mRNA processing factor (Bcas2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001106458.2

ORF Insert Sequence:

ATGGCGGGCA CGGGCTTGGT AGCGGGGGAG GTGGTAGTGG ATGCGCTACC CTACTTTGAC 
CAAGGCTATG AAGCGCCTGG CGTGCGGGAA GCGGCTGCGG CCCTGGTGGA GGAGGAAACG 
CGCAGATACC GACCTACCAA GAACTACCTG AGCTACCTGA CAGCCCCAGA TTATTCTGCC 
TTTGAAACAG ACATAATGAG AAATGAGTTT GAAAGACTGG CTGCTCGACA ACCAATTGAA 
TTACTCAGCA TGAAGCGATA TGAACTGCCA GCCCCTTCCT CGGGTCAAAA AAATGACATC 
ACTGCGTGGC AAGACTGTGT AAACAATTCT ATGGCCCAGC TGGAGCATCA GGCAGTGCGG 
ATTGAGAATC TGGAACTGAT GTCACAGCAT GGGTGTAACG CCTGGAAAGT GTACAACGAA 
AACCTTGTTC ACATGATTGA ACATGCACAG AAAGAGCTTC AGAAGTTAAG GAAGCATATT 
CAGGATTTAA ACTGGCAGCG CAAGAACATG CAGCTTACAG CTGGGGCTAA GCTGAGAGAA 
ATGGAGTCAA ATTGGGTGTC GCTGGTGAGT AAGAACTATG AGATTGAGCG GACTATTGTA 
CAGTTGGAGA ACGAGATCTA TCAAATCAAG CAGCAGCACG GTGAGGCAAA CAAGGAAAAC 
ATCCGCCAAG ACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cryo-EM structure of a human spliceosome activated for step 2 of splicing.
Nature542(7641)318-323(2017 02)
Bertram K,Agafonov DE,Liu WT,Dybkov O,Will CL,Hartmuth K,Urlaub H,Kastner B,Stark H,L?hrmann R

PRP19 transforms into a sensor of RPA-ssDNA after DNA damage and drives ATR activation via a ubiquitin-mediated circuitry.
Molecular cell53(2)235-246(2014 Jan)
Mar?chal A,Li JM,Ji XY,Wu CS,Yazinski SA,Nguyen HD,Liu S,Jim?nez AE,Jin J,Zou L

Splicing and beyond: the many faces of the Prp19 complex.
Biochimica et biophysica acta1833(10)2126-34(2013 Oct)
Chanarat S,Str??er K

Amplification of the BCAS2 gene at chromosome 1p13.3-21 in human primary breast cancer.
Cancer letters185(2)219-23(2002 Nov)
Maass N,R?sel F,Schem C,Hitomi J,Jonat W,Nagasaki K