Tnni1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tnni1
Entrez Gene ID	29388
Full Name	troponin I1, slow skeletal type
General protein information	Preferred Names troponin I1, slow skeletal type Names troponin I, slow skeletal muscle Troponin I, slow isoform troponin I type 1 (skeletal, slow) troponin I, skeletal, slow 1 troponin I, slow-twitch isoform
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 13 Map Location: 13q13
Summary	thin filament contractile regulatory protein; inhibits contractile interaction of myosin and actin [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017598771.1	XP_017454260.1	troponin I, slow skeletal muscle isoform X1
NM_017184.1	NP_058880.1	troponin I, slow skeletal muscle
XM_006249864.2	XP_006249926.1	troponin I, slow skeletal muscle isoform X2
XM_006249862.3	XP_006249924.1	troponin I, slow skeletal muscle isoform X1
XM_006249865.2	XP_006249927.1	troponin I, slow skeletal muscle isoform X3
XM_006249866.2	XP_006249928.1	troponin I, slow skeletal muscle isoform X3
XM_006249868.2	XP_006249930.1	troponin I, slow skeletal muscle isoform X4
XM_006249869.3	XP_006249931.1	troponin I, slow skeletal muscle isoform X4

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Tnni1	NP_058880.1
Gallus gallus (chicken)	TNNI1	XP_004934896.1
Pan troglodytes (chimpanzee)	TNNI1	XP_525019.2
Canis lupus familiaris (dog)	TNNI1	XP_003639192.1
Mus musculus (house mouse)	Tnni1	NP_001106173.1
Danio rerio (zebrafish)	tnni1b	NP_001008613.1
Homo sapiens (human)	TNNI1	NP_003272.3
	TNNI1	XP_001108591.1
Bos taurus (cattle)	TNNI1	XP_002694161.1
Xenopus tropicalis (tropical clawed frog)	LOC100125108	NP_001096486.1

Gene Ontology
Bibliography

Related articles in PubMed

The concerted movement of the switch region of Troponin I in cardiac muscle thin filaments as tracked by conventional and pulsed (DEER) EPR.
Potluri PR, Chamoun J, Cooke JA, Badr M, Guse JA, Rayes R, Cordina NM, McCamey D, Fajer PG, Brown LJ
Journal of structural biology200(3)376-387(2017 Dec)

Troponin I Mutations R146G and R21C Alter Cardiac Troponin Function, Contractile Properties, and Modulation by Protein Kinase A (PKA)-mediated Phosphorylation.
Cheng Y, Rao V, Tu AY, Lindert S, Wang D, Oxenford L, McCulloch AD, McCammon JA, Regnier M
The Journal of biological chemistry290(46)27749-66(2015 Nov)

Cell type-specific expression of endogenous cardiac Troponin I antisense RNA in the neonatal rat heart.
Voigtsberger S, Bartsch H, Baumann G, Luther HP
Molecular and cellular biochemistry324(1-2)1-11(2009 Apr)

Reduced force production during low blood flow to the heart correlates with altered troponin I phosphorylation.
Christopher B, Pizarro GO, Nicholson B, Yuen S, Hoit BD, Ogut O
Journal of muscle research and cell motility30(3-4)111-23(2009)

Overexpression of TEAD-1 in transgenic mouse striated muscles produces a slower skeletal muscle contractile phenotype.
Tsika RW, Schramm C, Simmer G, Fitzsimons DP, Moss RL, Ji J
The Journal of biological chemistry283(52)36154-67(2008 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

these results suggest that cTnI(R146G) and cTnI(R21C) blunt PKA modulation of activation and relaxation kinetics by prohibiting cardiac-specific N-terminal interaction with the cTnI inhibitory peptide.
Title: Troponin I Mutations R146G and R21C Alter Cardiac Troponin Function, Contractile Properties, and Modulation by Protein Kinase A (PKA)-mediated Phosphorylation.

observed higher relative level of natural antisense RNA in neonatal cardiac fibroblasts compared to neonatal cardiomyocytes, pointing to a cell type-specific expression of sense and antisense RNA
Title: Cell type-specific expression of endogenous cardiac Troponin I antisense RNA in the neonatal rat heart.

These findings suggest that altered TnI function, due to changes in the distribution of phosphorylated sites, is an early contributor to reduced contractility of the heart.
Title: Reduced force production during low blood flow to the heart correlates with altered troponin I phosphorylation.

The negative inotropic effect of HIV gp120 is mediated by a novel signaling pathway that begins with binding to a cardiac myocyte CXCR4 receptor, followed by phosphorylation of both p38 MAP kinase and Troponin I.
Title: CXCR4 receptor antagonist blocks cardiac myocyte p38 MAP kinase phosphorylation by HIV gp120.

Study identifies tyrosine phosphorylation as a novel protein kinase Cdelta (PKCdelta) activation mechanism that modifies PKCdelta-dependent phosphorylation of cardiac troponin I.
Title: Tyrosine phosphorylation modifies protein kinase C delta-dependent phosphorylation of cardiac troponin I.

The following Tnni1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tnni1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa03157	NM_017184.1 Latest version!	Rattus norvegicus troponin I1, slow skeletal type (Tnni1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa35362	XM_006249862.3 Latest version!	Rattus norvegicus troponin I1, slow skeletal type (Tnni1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
ORa35362	XM_017598771.1 Latest version!	Rattus norvegicus troponin I1, slow skeletal type (Tnni1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
ORa35363	XM_006249864.2 Latest version!	Rattus norvegicus troponin I1, slow skeletal type (Tnni1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
ORa35364	XM_006249866.2 Latest version!	Rattus norvegicus troponin I1, slow skeletal type (Tnni1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa35364	XM_006249865.2 Latest version!	Rattus norvegicus troponin I1, slow skeletal type (Tnni1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa35365	XM_006249868.2 Latest version!	Rattus norvegicus troponin I1, slow skeletal type (Tnni1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
ORa35365	XM_006249869.3 Latest version!	Rattus norvegicus troponin I1, slow skeletal type (Tnni1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tnni1 ( NM_017184.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_058880.1 Rattus norvegicus troponin I1, slow skeletal type (Tnni1), mRNA.

Price & Availability↑

CloneID	ORa03157
Clone ID Related Accession (Same CDS sequence)	NM_017184.1
Accession Version	NM_017184.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 564bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Rattus norvegicus(Norway rat)
Product	troponin I, slow skeletal muscle
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from J04993.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: J04993.1, BF525158.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689600, SAMEA2689602 [ECO:0000348] ##Evidence-Data-END##

ATGCCGGAAG TTGAGAGGAA ATCTAAGATC ACTGCCTCCC GTAAACTCAT GCTGAAGAGC 
CTGATGCTAG CCAAGGCCAA GGAGTGCTGG GAGCAGGAGC ACGAGGAGCG AGAGGCTGAG 
AAGGTGCGTT ACCTCTCGGA GCGCATCCCT ACGCTGCAGA CCCGCGGTCT TTCCCTCAGC 
GCCCTTCAGG ACTTATGCCG AGAGCTCCAT GCCAAGGTGG AGGTGGTGGA TGAGGAGCGC 
TATGATATCG AAGCCAAATG CCTCCACAAC ACGAGAGAGA TCAAGGACCT GAAGCTGAAG 
GTGCTGGACC TCCGTGGGAA GTTCAAGCGT CCACCCCTGC GCCGGGTCCG TGTCTCAGCC 
GACGCCATGC TCCGAGCCCT ACTGGGTTCC AAACACAAGG TGTCCATGGA TCTGCGGGCC 
AACCTCAAGT CTGTGAAGAA AGAAGACACA GAAAAGGAGC GGCCAGTGGA GGTGGGAGAC 
TGGAGGAAGA ACGTGGAGGC CATGTCTGGC ATGGAAGGTC GCAAGAAGAT GTTCGATGCT 
GCCAAGTCCC CAACCTTGCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058880.1
CDS	44..607
Translation	MPEVERKSKITASRKLMLKSLMLAKAKECWEQEHEEREAEKVRYLSERIPTLQTRGLSLSALQDLCRELHAKVEVVDEERYDIEAKCLHNTREIKDLKLKVLDLRGKFKRPPLRRVRVSADAMLRALLGSKHKVSMDLRANLKSVKKEDTEKERPVEVGDWRKNVEAMSGMEGRKKMFDAAKSPTLQ

Target ORF information:

RefSeq Version	NM_017184.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus troponin I1, slow skeletal type (Tnni1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017184.1

ORF Insert Sequence:

ATGCCGGAAG TTGAGAGGAA ATCTAAGATC ACTGCCTCCC GTAAACTCAT GCTGAAGAGC 
CTGATGCTAG CCAAGGCCAA GGAGTGCTGG GAGCAGGAGC ACGAGGAGCG AGAGGCTGAG 
AAGGTGCGTT ACCTCTCGGA GCGCATCCCT ACGCTGCAGA CCCGCGGTCT TTCCCTCAGC 
GCCCTTCAGG ACTTATGCCG AGAGCTCCAT GCCAAGGTGG AGGTGGTGGA TGAGGAGCGC 
TATGATATCG AAGCCAAATG CCTCCACAAC ACGAGAGAGA TCAAGGACCT GAAGCTGAAG 
GTGCTGGACC TCCGTGGGAA GTTCAAGCGT CCACCCCTGC GCCGGGTCCG TGTCTCAGCC 
GACGCCATGC TCCGAGCCCT ACTGGGTTCC AAACACAAGG TGTCCATGGA TCTGCGGGCC 
AACCTCAAGT CTGTGAAGAA AGAAGACACA GAAAAGGAGC GGCCAGTGGA GGTGGGAGAC 
TGGAGGAAGA ACGTGGAGGC CATGTCTGGC ATGGAAGGTC GCAAGAAGAT GTTCGATGCT 
GCCAAGTCCC CAACCTTGCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tnni1 ( XM_017598771.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017454260.1 Rattus norvegicus troponin I1, slow skeletal type (Tnni1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa35362
Clone ID Related Accession (Same CDS sequence)	XM_017598771.1 , XM_006249862.3
Accession Version	XM_017598771.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 711bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	troponin I, slow skeletal muscle isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGTTT TGGGCTTTTC ACGAAGGCTG CACATCTGTG CCCTCATTTG GTCCCGCTGG 
AGCTGTGGAC TAACCACAGT TGTCAGCGTG CTCCTCCGCA GATGCAGAAG GTACCTGTGG 
GAGGACATCT GCAGTTTCCC GAGGGATCCT TCCAGCAGTG AAAGGAAATC TAAGATCACT 
GCCTCCCGTA AACTCATGCT GAAGAGCCTG ATGCTAGCCA AGGCCAAGGA GTGCTGGGAG 
CAGGAGCACG AGGAGCGAGA GGCTGAGAAG GTGCGTTACC TCTCGGAGCG CATCCCTACG 
CTGCAGACCC GCGGTCTTTC CCTCAGCGCC CTTCAGGACT TATGCCGAGA GCTCCATGCC 
AAGGTGGAGG TGGTGGATGA GGAGCGCTAT GATATCGAAG CCAAATGCCT CCACAACACG 
AGAGAGATCA AGGACCTGAA GCTGAAGGTG CTGGACCTCC GTGGGAAGTT CAAGCGTCCA 
CCCCTGCGCC GGGTCCGTGT CTCAGCCGAC GCCATGCTCC GAGCCCTACT GGGTTCCAAA 
CACAAGGTGT CCATGGATCT GCGGGCCAAC CTCAAGTCTG TGAAGAAAGA AGACACAGAA 
AAGGAGCGGC CAGTGGAGGT GGGAGACTGG AGGAAGAACG TGGAGGCCAT GTCTGGCATG 
GAAGGCCGCA AGAAGATGTT CGATGCTGCC AAGTCCCCAA CCTCGCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017454260.1
CDS	1406..2116
Translation	MPVLGFSRRLHICALIWSRWSCGLTTVVSVLLRRCRRYLWEDICSFPRDPSSSERKSKITASRKLMLKSLMLAKAKECWEQEHEEREAEKVRYLSERIPTLQTRGLSLSALQDLCRELHAKVEVVDEERYDIEAKCLHNTREIKDLKLKVLDLRGKFKRPPLRRVRVSADAMLRALLGSKHKVSMDLRANLKSVKKEDTEKERPVEVGDWRKNVEAMSGMEGRKKMFDAAKSPTSQ

Target ORF information:

RefSeq Version	XM_017598771.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus troponin I1, slow skeletal type (Tnni1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017598771.1

ORF Insert Sequence:

ATGCCTGTTT TGGGCTTTTC ACGAAGGCTG CACATCTGTG CCCTCATTTG GTCCCGCTGG 
AGCTGTGGAC TAACCACAGT TGTCAGCGTG CTCCTCCGCA GATGCAGAAG GTACCTGTGG 
GAGGACATCT GCAGTTTCCC GAGGGATCCT TCCAGCAGTG AAAGGAAATC TAAGATCACT 
GCCTCCCGTA AACTCATGCT GAAGAGCCTG ATGCTAGCCA AGGCCAAGGA GTGCTGGGAG 
CAGGAGCACG AGGAGCGAGA GGCTGAGAAG GTGCGTTACC TCTCGGAGCG CATCCCTACG 
CTGCAGACCC GCGGTCTTTC CCTCAGCGCC CTTCAGGACT TATGCCGAGA GCTCCATGCC 
AAGGTGGAGG TGGTGGATGA GGAGCGCTAT GATATCGAAG CCAAATGCCT CCACAACACG 
AGAGAGATCA AGGACCTGAA GCTGAAGGTG CTGGACCTCC GTGGGAAGTT CAAGCGTCCA 
CCCCTGCGCC GGGTCCGTGT CTCAGCCGAC GCCATGCTCC GAGCCCTACT GGGTTCCAAA 
CACAAGGTGT CCATGGATCT GCGGGCCAAC CTCAAGTCTG TGAAGAAAGA AGACACAGAA 
AAGGAGCGGC CAGTGGAGGT GGGAGACTGG AGGAAGAACG TGGAGGCCAT GTCTGGCATG 
GAAGGCCGCA AGAAGATGTT CGATGCTGCC AAGTCCCCAA CCTCGCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tnni1 ( XM_006249862.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006249924.1 Rattus norvegicus troponin I1, slow skeletal type (Tnni1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa35362
Clone ID Related Accession (Same CDS sequence)	XM_017598771.1 , XM_006249862.3
Accession Version	XM_006249862.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 711bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	troponin I, slow skeletal muscle isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006249862.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGTTT TGGGCTTTTC ACGAAGGCTG CACATCTGTG CCCTCATTTG GTCCCGCTGG 
AGCTGTGGAC TAACCACAGT TGTCAGCGTG CTCCTCCGCA GATGCAGAAG GTACCTGTGG 
GAGGACATCT GCAGTTTCCC GAGGGATCCT TCCAGCAGTG AAAGGAAATC TAAGATCACT 
GCCTCCCGTA AACTCATGCT GAAGAGCCTG ATGCTAGCCA AGGCCAAGGA GTGCTGGGAG 
CAGGAGCACG AGGAGCGAGA GGCTGAGAAG GTGCGTTACC TCTCGGAGCG CATCCCTACG 
CTGCAGACCC GCGGTCTTTC CCTCAGCGCC CTTCAGGACT TATGCCGAGA GCTCCATGCC 
AAGGTGGAGG TGGTGGATGA GGAGCGCTAT GATATCGAAG CCAAATGCCT CCACAACACG 
AGAGAGATCA AGGACCTGAA GCTGAAGGTG CTGGACCTCC GTGGGAAGTT CAAGCGTCCA 
CCCCTGCGCC GGGTCCGTGT CTCAGCCGAC GCCATGCTCC GAGCCCTACT GGGTTCCAAA 
CACAAGGTGT CCATGGATCT GCGGGCCAAC CTCAAGTCTG TGAAGAAAGA AGACACAGAA 
AAGGAGCGGC CAGTGGAGGT GGGAGACTGG AGGAAGAACG TGGAGGCCAT GTCTGGCATG 
GAAGGCCGCA AGAAGATGTT CGATGCTGCC AAGTCCCCAA CCTCGCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006249924.1
CDS	1405..2115
Translation	MPVLGFSRRLHICALIWSRWSCGLTTVVSVLLRRCRRYLWEDICSFPRDPSSSERKSKITASRKLMLKSLMLAKAKECWEQEHEEREAEKVRYLSERIPTLQTRGLSLSALQDLCRELHAKVEVVDEERYDIEAKCLHNTREIKDLKLKVLDLRGKFKRPPLRRVRVSADAMLRALLGSKHKVSMDLRANLKSVKKEDTEKERPVEVGDWRKNVEAMSGMEGRKKMFDAAKSPTSQ

Target ORF information:

RefSeq Version	XM_006249862.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus troponin I1, slow skeletal type (Tnni1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006249862.3

ORF Insert Sequence:

ATGCCTGTTT TGGGCTTTTC ACGAAGGCTG CACATCTGTG CCCTCATTTG GTCCCGCTGG 
AGCTGTGGAC TAACCACAGT TGTCAGCGTG CTCCTCCGCA GATGCAGAAG GTACCTGTGG 
GAGGACATCT GCAGTTTCCC GAGGGATCCT TCCAGCAGTG AAAGGAAATC TAAGATCACT 
GCCTCCCGTA AACTCATGCT GAAGAGCCTG ATGCTAGCCA AGGCCAAGGA GTGCTGGGAG 
CAGGAGCACG AGGAGCGAGA GGCTGAGAAG GTGCGTTACC TCTCGGAGCG CATCCCTACG 
CTGCAGACCC GCGGTCTTTC CCTCAGCGCC CTTCAGGACT TATGCCGAGA GCTCCATGCC 
AAGGTGGAGG TGGTGGATGA GGAGCGCTAT GATATCGAAG CCAAATGCCT CCACAACACG 
AGAGAGATCA AGGACCTGAA GCTGAAGGTG CTGGACCTCC GTGGGAAGTT CAAGCGTCCA 
CCCCTGCGCC GGGTCCGTGT CTCAGCCGAC GCCATGCTCC GAGCCCTACT GGGTTCCAAA 
CACAAGGTGT CCATGGATCT GCGGGCCAAC CTCAAGTCTG TGAAGAAAGA AGACACAGAA 
AAGGAGCGGC CAGTGGAGGT GGGAGACTGG AGGAAGAACG TGGAGGCCAT GTCTGGCATG 
GAAGGCCGCA AGAAGATGTT CGATGCTGCC AAGTCCCCAA CCTCGCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tnni1 ( XM_006249864.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006249926.1 Rattus norvegicus troponin I1, slow skeletal type (Tnni1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa35363
Clone ID Related Accession (Same CDS sequence)	XM_006249864.2
Accession Version	XM_006249864.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 693bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	troponin I, slow skeletal muscle isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006249864.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGGC TGCACATCTG TGCCCTCATT TGGTCCCGCT GGAGCTGTGG ACTAACCACA 
GTTGTCAGCG TGCTCCTCCG CAGATGCAGA AGGTACCTGT GGGAGGACAT CTGCAGTTTC 
CCGAGGGATC CTTCCAGCAG TGAAAGGAAA TCTAAGATCA CTGCCTCCCG TAAACTCATG 
CTGAAGAGCC TGATGCTAGC CAAGGCCAAG GAGTGCTGGG AGCAGGAGCA CGAGGAGCGA 
GAGGCTGAGA AGGTGCGTTA CCTCTCGGAG CGCATCCCTA CGCTGCAGAC CCGCGGTCTT 
TCCCTCAGCG CCCTTCAGGA CTTATGCCGA GAGCTCCATG CCAAGGTGGA GGTGGTGGAT 
GAGGAGCGCT ATGATATCGA AGCCAAATGC CTCCACAACA CGAGAGAGAT CAAGGACCTG 
AAGCTGAAGG TGCTGGACCT CCGTGGGAAG TTCAAGCGTC CACCCCTGCG CCGGGTCCGT 
GTCTCAGCCG ACGCCATGCT CCGAGCCCTA CTGGGTTCCA AACACAAGGT GTCCATGGAT 
CTGCGGGCCA ACCTCAAGTC TGTGAAGAAA GAAGACACAG AAAAGGAGCG GCCAGTGGAG 
GTGGGAGACT GGAGGAAGAA CGTGGAGGCC ATGTCTGGCA TGGAAGGCCG CAAGAAGATG 
TTCGATGCTG CCAAGTCCCC AACCTCGCAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006249926.1
CDS	439..1131
Translation	MERLHICALIWSRWSCGLTTVVSVLLRRCRRYLWEDICSFPRDPSSSERKSKITASRKLMLKSLMLAKAKECWEQEHEEREAEKVRYLSERIPTLQTRGLSLSALQDLCRELHAKVEVVDEERYDIEAKCLHNTREIKDLKLKVLDLRGKFKRPPLRRVRVSADAMLRALLGSKHKVSMDLRANLKSVKKEDTEKERPVEVGDWRKNVEAMSGMEGRKKMFDAAKSPTSQ

Target ORF information:

RefSeq Version	XM_006249864.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus troponin I1, slow skeletal type (Tnni1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006249864.2

ORF Insert Sequence:

ATGGAGAGGC TGCACATCTG TGCCCTCATT TGGTCCCGCT GGAGCTGTGG ACTAACCACA 
GTTGTCAGCG TGCTCCTCCG CAGATGCAGA AGGTACCTGT GGGAGGACAT CTGCAGTTTC 
CCGAGGGATC CTTCCAGCAG TGAAAGGAAA TCTAAGATCA CTGCCTCCCG TAAACTCATG 
CTGAAGAGCC TGATGCTAGC CAAGGCCAAG GAGTGCTGGG AGCAGGAGCA CGAGGAGCGA 
GAGGCTGAGA AGGTGCGTTA CCTCTCGGAG CGCATCCCTA CGCTGCAGAC CCGCGGTCTT 
TCCCTCAGCG CCCTTCAGGA CTTATGCCGA GAGCTCCATG CCAAGGTGGA GGTGGTGGAT 
GAGGAGCGCT ATGATATCGA AGCCAAATGC CTCCACAACA CGAGAGAGAT CAAGGACCTG 
AAGCTGAAGG TGCTGGACCT CCGTGGGAAG TTCAAGCGTC CACCCCTGCG CCGGGTCCGT 
GTCTCAGCCG ACGCCATGCT CCGAGCCCTA CTGGGTTCCA AACACAAGGT GTCCATGGAT 
CTGCGGGCCA ACCTCAAGTC TGTGAAGAAA GAAGACACAG AAAAGGAGCG GCCAGTGGAG 
GTGGGAGACT GGAGGAAGAA CGTGGAGGCC ATGTCTGGCA TGGAAGGCCG CAAGAAGATG 
TTCGATGCTG CCAAGTCCCC AACCTCGCAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tnni1 ( XM_006249865.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006249927.1 Rattus norvegicus troponin I1, slow skeletal type (Tnni1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa35364
Clone ID Related Accession (Same CDS sequence)	XM_006249865.2 , XM_006249866.2
Accession Version	XM_006249865.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 564bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	troponin I, slow skeletal muscle isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006249865.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGAAG TTGAGAGGAA ATCTAAGATC ACTGCCTCCC GTAAACTCAT GCTGAAGAGC 
CTGATGCTAG CCAAGGCCAA GGAGTGCTGG GAGCAGGAGC ACGAGGAGCG AGAGGCTGAG 
AAGGTGCGTT ACCTCTCGGA GCGCATCCCT ACGCTGCAGA CCCGCGGTCT TTCCCTCAGC 
GCCCTTCAGG ACTTATGCCG AGAGCTCCAT GCCAAGGTGG AGGTGGTGGA TGAGGAGCGC 
TATGATATCG AAGCCAAATG CCTCCACAAC ACGAGAGAGA TCAAGGACCT GAAGCTGAAG 
GTGCTGGACC TCCGTGGGAA GTTCAAGCGT CCACCCCTGC GCCGGGTCCG TGTCTCAGCC 
GACGCCATGC TCCGAGCCCT ACTGGGTTCC AAACACAAGG TGTCCATGGA TCTGCGGGCC 
AACCTCAAGT CTGTGAAGAA AGAAGACACA GAAAAGGAGC GGCCAGTGGA GGTGGGAGAC 
TGGAGGAAGA ACGTGGAGGC CATGTCTGGC ATGGAAGGCC GCAAGAAGAT GTTCGATGCT 
GCCAAGTCCC CAACCTCGCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006249927.1
CDS	173..736
Translation	MPEVERKSKITASRKLMLKSLMLAKAKECWEQEHEEREAEKVRYLSERIPTLQTRGLSLSALQDLCRELHAKVEVVDEERYDIEAKCLHNTREIKDLKLKVLDLRGKFKRPPLRRVRVSADAMLRALLGSKHKVSMDLRANLKSVKKEDTEKERPVEVGDWRKNVEAMSGMEGRKKMFDAAKSPTSQ

Target ORF information:

RefSeq Version	XM_006249865.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus troponin I1, slow skeletal type (Tnni1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006249865.2

ORF Insert Sequence:

ATGCCGGAAG TTGAGAGGAA ATCTAAGATC ACTGCCTCCC GTAAACTCAT GCTGAAGAGC 
CTGATGCTAG CCAAGGCCAA GGAGTGCTGG GAGCAGGAGC ACGAGGAGCG AGAGGCTGAG 
AAGGTGCGTT ACCTCTCGGA GCGCATCCCT ACGCTGCAGA CCCGCGGTCT TTCCCTCAGC 
GCCCTTCAGG ACTTATGCCG AGAGCTCCAT GCCAAGGTGG AGGTGGTGGA TGAGGAGCGC 
TATGATATCG AAGCCAAATG CCTCCACAAC ACGAGAGAGA TCAAGGACCT GAAGCTGAAG 
GTGCTGGACC TCCGTGGGAA GTTCAAGCGT CCACCCCTGC GCCGGGTCCG TGTCTCAGCC 
GACGCCATGC TCCGAGCCCT ACTGGGTTCC AAACACAAGG TGTCCATGGA TCTGCGGGCC 
AACCTCAAGT CTGTGAAGAA AGAAGACACA GAAAAGGAGC GGCCAGTGGA GGTGGGAGAC 
TGGAGGAAGA ACGTGGAGGC CATGTCTGGC ATGGAAGGCC GCAAGAAGAT GTTCGATGCT 
GCCAAGTCCC CAACCTCGCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tnni1 ( XM_006249866.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006249928.1 Rattus norvegicus troponin I1, slow skeletal type (Tnni1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ORa35364
Clone ID Related Accession (Same CDS sequence)	XM_006249865.2 , XM_006249866.2
Accession Version	XM_006249866.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 564bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	troponin I, slow skeletal muscle isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006249866.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGAAG TTGAGAGGAA ATCTAAGATC ACTGCCTCCC GTAAACTCAT GCTGAAGAGC 
CTGATGCTAG CCAAGGCCAA GGAGTGCTGG GAGCAGGAGC ACGAGGAGCG AGAGGCTGAG 
AAGGTGCGTT ACCTCTCGGA GCGCATCCCT ACGCTGCAGA CCCGCGGTCT TTCCCTCAGC 
GCCCTTCAGG ACTTATGCCG AGAGCTCCAT GCCAAGGTGG AGGTGGTGGA TGAGGAGCGC 
TATGATATCG AAGCCAAATG CCTCCACAAC ACGAGAGAGA TCAAGGACCT GAAGCTGAAG 
GTGCTGGACC TCCGTGGGAA GTTCAAGCGT CCACCCCTGC GCCGGGTCCG TGTCTCAGCC 
GACGCCATGC TCCGAGCCCT ACTGGGTTCC AAACACAAGG TGTCCATGGA TCTGCGGGCC 
AACCTCAAGT CTGTGAAGAA AGAAGACACA GAAAAGGAGC GGCCAGTGGA GGTGGGAGAC 
TGGAGGAAGA ACGTGGAGGC CATGTCTGGC ATGGAAGGCC GCAAGAAGAT GTTCGATGCT 
GCCAAGTCCC CAACCTCGCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006249928.1
CDS	104..667
Translation	MPEVERKSKITASRKLMLKSLMLAKAKECWEQEHEEREAEKVRYLSERIPTLQTRGLSLSALQDLCRELHAKVEVVDEERYDIEAKCLHNTREIKDLKLKVLDLRGKFKRPPLRRVRVSADAMLRALLGSKHKVSMDLRANLKSVKKEDTEKERPVEVGDWRKNVEAMSGMEGRKKMFDAAKSPTSQ

Target ORF information:

RefSeq Version	XM_006249866.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus troponin I1, slow skeletal type (Tnni1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006249866.2

ORF Insert Sequence:

ATGCCGGAAG TTGAGAGGAA ATCTAAGATC ACTGCCTCCC GTAAACTCAT GCTGAAGAGC 
CTGATGCTAG CCAAGGCCAA GGAGTGCTGG GAGCAGGAGC ACGAGGAGCG AGAGGCTGAG 
AAGGTGCGTT ACCTCTCGGA GCGCATCCCT ACGCTGCAGA CCCGCGGTCT TTCCCTCAGC 
GCCCTTCAGG ACTTATGCCG AGAGCTCCAT GCCAAGGTGG AGGTGGTGGA TGAGGAGCGC 
TATGATATCG AAGCCAAATG CCTCCACAAC ACGAGAGAGA TCAAGGACCT GAAGCTGAAG 
GTGCTGGACC TCCGTGGGAA GTTCAAGCGT CCACCCCTGC GCCGGGTCCG TGTCTCAGCC 
GACGCCATGC TCCGAGCCCT ACTGGGTTCC AAACACAAGG TGTCCATGGA TCTGCGGGCC 
AACCTCAAGT CTGTGAAGAA AGAAGACACA GAAAAGGAGC GGCCAGTGGA GGTGGGAGAC 
TGGAGGAAGA ACGTGGAGGC CATGTCTGGC ATGGAAGGCC GCAAGAAGAT GTTCGATGCT 
GCCAAGTCCC CAACCTCGCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tnni1 ( XM_006249868.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006249930.1 Rattus norvegicus troponin I1, slow skeletal type (Tnni1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ORa35365
Clone ID Related Accession (Same CDS sequence)	XM_006249868.2 , XM_006249869.3
Accession Version	XM_006249868.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 516bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	troponin I, slow skeletal muscle isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006249868.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGA GCCTGATGCT AGCCAAGGCC AAGGAGTGCT GGGAGCAGGA GCACGAGGAG 
CGAGAGGCTG AGAAGGTGCG TTACCTCTCG GAGCGCATCC CTACGCTGCA GACCCGCGGT 
CTTTCCCTCA GCGCCCTTCA GGACTTATGC CGAGAGCTCC ATGCCAAGGT GGAGGTGGTG 
GATGAGGAGC GCTATGATAT CGAAGCCAAA TGCCTCCACA ACACGAGAGA GATCAAGGAC 
CTGAAGCTGA AGGTGCTGGA CCTCCGTGGG AAGTTCAAGC GTCCACCCCT GCGCCGGGTC 
CGTGTCTCAG CCGACGCCAT GCTCCGAGCC CTACTGGGTT CCAAACACAA GGTGTCCATG 
GATCTGCGGG CCAACCTCAA GTCTGTGAAG AAAGAAGACA CAGAAAAGGA GCGGCCAGTG 
GAGGTGGGAG ACTGGAGGAA GAACGTGGAG GCCATGTCTG GCATGGAAGG CCGCAAGAAG 
ATGTTCGATG CTGCCAAGTC CCCAACCTCG CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006249930.1
CDS	187..702
Translation	MLKSLMLAKAKECWEQEHEEREAEKVRYLSERIPTLQTRGLSLSALQDLCRELHAKVEVVDEERYDIEAKCLHNTREIKDLKLKVLDLRGKFKRPPLRRVRVSADAMLRALLGSKHKVSMDLRANLKSVKKEDTEKERPVEVGDWRKNVEAMSGMEGRKKMFDAAKSPTSQ

Target ORF information:

RefSeq Version	XM_006249868.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus troponin I1, slow skeletal type (Tnni1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006249868.2

ORF Insert Sequence:

ATGCTGAAGA GCCTGATGCT AGCCAAGGCC AAGGAGTGCT GGGAGCAGGA GCACGAGGAG 
CGAGAGGCTG AGAAGGTGCG TTACCTCTCG GAGCGCATCC CTACGCTGCA GACCCGCGGT 
CTTTCCCTCA GCGCCCTTCA GGACTTATGC CGAGAGCTCC ATGCCAAGGT GGAGGTGGTG 
GATGAGGAGC GCTATGATAT CGAAGCCAAA TGCCTCCACA ACACGAGAGA GATCAAGGAC 
CTGAAGCTGA AGGTGCTGGA CCTCCGTGGG AAGTTCAAGC GTCCACCCCT GCGCCGGGTC 
CGTGTCTCAG CCGACGCCAT GCTCCGAGCC CTACTGGGTT CCAAACACAA GGTGTCCATG 
GATCTGCGGG CCAACCTCAA GTCTGTGAAG AAAGAAGACA CAGAAAAGGA GCGGCCAGTG 
GAGGTGGGAG ACTGGAGGAA GAACGTGGAG GCCATGTCTG GCATGGAAGG CCGCAAGAAG 
ATGTTCGATG CTGCCAAGTC CCCAACCTCG CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tnni1 ( XM_006249869.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006249931.1 Rattus norvegicus troponin I1, slow skeletal type (Tnni1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	ORa35365
Clone ID Related Accession (Same CDS sequence)	XM_006249868.2 , XM_006249869.3
Accession Version	XM_006249869.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 516bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	troponin I, slow skeletal muscle isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005112.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006249869.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGAAGA GCCTGATGCT AGCCAAGGCC AAGGAGTGCT GGGAGCAGGA GCACGAGGAG 
CGAGAGGCTG AGAAGGTGCG TTACCTCTCG GAGCGCATCC CTACGCTGCA GACCCGCGGT 
CTTTCCCTCA GCGCCCTTCA GGACTTATGC CGAGAGCTCC ATGCCAAGGT GGAGGTGGTG 
GATGAGGAGC GCTATGATAT CGAAGCCAAA TGCCTCCACA ACACGAGAGA GATCAAGGAC 
CTGAAGCTGA AGGTGCTGGA CCTCCGTGGG AAGTTCAAGC GTCCACCCCT GCGCCGGGTC 
CGTGTCTCAG CCGACGCCAT GCTCCGAGCC CTACTGGGTT CCAAACACAA GGTGTCCATG 
GATCTGCGGG CCAACCTCAA GTCTGTGAAG AAAGAAGACA CAGAAAAGGA GCGGCCAGTG 
GAGGTGGGAG ACTGGAGGAA GAACGTGGAG GCCATGTCTG GCATGGAAGG CCGCAAGAAG 
ATGTTCGATG CTGCCAAGTC CCCAACCTCG CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006249931.1
CDS	109..624
Translation	MLKSLMLAKAKECWEQEHEEREAEKVRYLSERIPTLQTRGLSLSALQDLCRELHAKVEVVDEERYDIEAKCLHNTREIKDLKLKVLDLRGKFKRPPLRRVRVSADAMLRALLGSKHKVSMDLRANLKSVKKEDTEKERPVEVGDWRKNVEAMSGMEGRKKMFDAAKSPTSQ

Target ORF information:

RefSeq Version	XM_006249869.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus troponin I1, slow skeletal type (Tnni1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006249869.3

ORF Insert Sequence:

ATGCTGAAGA GCCTGATGCT AGCCAAGGCC AAGGAGTGCT GGGAGCAGGA GCACGAGGAG 
CGAGAGGCTG AGAAGGTGCG TTACCTCTCG GAGCGCATCC CTACGCTGCA GACCCGCGGT 
CTTTCCCTCA GCGCCCTTCA GGACTTATGC CGAGAGCTCC ATGCCAAGGT GGAGGTGGTG 
GATGAGGAGC GCTATGATAT CGAAGCCAAA TGCCTCCACA ACACGAGAGA GATCAAGGAC 
CTGAAGCTGA AGGTGCTGGA CCTCCGTGGG AAGTTCAAGC GTCCACCCCT GCGCCGGGTC 
CGTGTCTCAG CCGACGCCAT GCTCCGAGCC CTACTGGGTT CCAAACACAA GGTGTCCATG 
GATCTGCGGG CCAACCTCAA GTCTGTGAAG AAAGAAGACA CAGAAAAGGA GCGGCCAGTG 
GAGGTGGGAG ACTGGAGGAA GAACGTGGAG GCCATGTCTG GCATGGAAGG CCGCAAGAAG 
ATGTTCGATG CTGCCAAGTC CCCAACCTCG CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The concerted movement of the switch region of Troponin I in cardiac muscle thin filaments as tracked by conventional and pulsed (DEER) EPR.
Journal of structural biology200(3)376-387(2017 Dec)
Potluri PR,Chamoun J,Cooke JA,Badr M,Guse JA,Rayes R,Cordina NM,McCamey D,Fajer PG,Brown LJ

Troponin I Mutations R146G and R21C Alter Cardiac Troponin Function, Contractile Properties, and Modulation by Protein Kinase A (PKA)-mediated Phosphorylation.
The Journal of biological chemistry290(46)27749-66(2015 Nov)
Cheng Y,Rao V,Tu AY,Lindert S,Wang D,Oxenford L,McCulloch AD,McCammon JA,Regnier M

Cell type-specific expression of endogenous cardiac Troponin I antisense RNA in the neonatal rat heart.
Molecular and cellular biochemistry324(1-2)1-11(2009 Apr)
Voigtsberger S,Bartsch H,Baumann G,Luther HP

Reduced force production during low blood flow to the heart correlates with altered troponin I phosphorylation.
Journal of muscle research and cell motility30(3-4)111-23(2009)
Christopher B,Pizarro GO,Nicholson B,Yuen S,Hoit BD,Ogut O

Overexpression of TEAD-1 in transgenic mouse striated muscles produces a slower skeletal muscle contractile phenotype.
The Journal of biological chemistry283(52)36154-67(2008 Dec)
Tsika RW,Schramm C,Simmer G,Fitzsimons DP,Moss RL,Ji J

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Tnni1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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