Tnni1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tnni1
Entrez Gene ID	21952
Full Name	troponin I, skeletal, slow 1
Synonyms	2700018B22Rik,AI747285,ssTnI
General protein information	Preferred Names troponin I, skeletal, slow 1 Names troponin I, slow skeletal muscle troponin I, slow-twitch isoform
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 1 Map Location: 1 E4\|1 59.28 cM

mRNA and Protein(s)

mRNA	Protein	Name
XM_006529382.3	XP_006529445.1	troponin I, slow skeletal muscle isoform X1
NM_001112702.1	NP_001106173.1	troponin I, slow skeletal muscle
XM_011247974.3	XP_011246276.1	troponin I, slow skeletal muscle isoform X1
XM_011247974.2	XP_011246276.1	troponin I, slow skeletal muscle isoform X1
NM_021467.5	NP_067442.1	troponin I, slow skeletal muscle

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Tnni1	NP_058880.1
Gallus gallus (chicken)	TNNI1	XP_004934896.1
Pan troglodytes (chimpanzee)	TNNI1	XP_525019.2
Canis lupus familiaris (dog)	TNNI1	XP_003639192.1
Mus musculus (house mouse)	Tnni1	NP_001106173.1
Danio rerio (zebrafish)	tnni1b	NP_001008613.1
Homo sapiens (human)	TNNI1	NP_003272.3
	TNNI1	XP_001108591.1
Bos taurus (cattle)	TNNI1	XP_002694161.1
Xenopus tropicalis (tropical clawed frog)	LOC100125108	NP_001096486.1

Gene Ontology
Bibliography

Related articles in PubMed

Inactivation of the myogenic bHLH gene MRF4 results in up-regulation of myogenin and rib anomalies.
Zhang W, Behringer RR, Olson EN
Genes & development9(11)1388-99(1995 Jun)

Transcriptional enhancer factor 1 disruption by a retroviral gene trap leads to heart defects and embryonic lethality in mice.
Chen Z, Friedrich GA, Soriano P
Genes & development8(19)2293-301(1994 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the up-stream domain ( approximately 1,800 bp) of mouse fetal TnI gene is been cloned and characterized
Title: Functional characterization of mouse fetal TnI gene promoters in myocardial cells.

Phosphorylation of troponin I was significantly reduced in Galphaq-overexpressing mice compared to wild type.
Title: Reduced troponin I phosphorylation and increased Ca(2+)-dependent ATP-consumption in triton X-skinned fiber preparations from Galphaq overexpressor mice.

K+-depolarization increased Tnni1 and Acta1 mRNA levels in both differentiated C2C12 and rat skeletal muscle cells in primary culture
Title: Differential gene expression in skeletal muscle cells after membrane depolarization.

a localized N-terminal region of TnI comprised of amino acids 33-80, which interacts with C-terminal regions of cTnC and cTnT, is of particular significance in transducing signaling of thin filament activation by strong cross-bridges
Title: Identification of a region of troponin I important in signaling cross-bridge-dependent activation of cardiac myofilaments.

data demonstrate that thyroid hormone can alter the time course of ssTnI gene expression in cultured cardiac myocytes and TnI gene regulation is also controlled by some unknown programmed events inside of cardiac myocytes
Title: Thyroid hormone inhibits slow skeletal TnI expression in cardiac TnI-null myocardial cells.

The following Tnni1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tnni1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu23491	NM_001112702.1 Latest version!	Mus musculus troponin I, skeletal, slow 1 (Tnni1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu23491	NM_021467.5 Latest version!	Mus musculus troponin I, skeletal, slow 1 (Tnni1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu23491	XM_006529382.3 Latest version!	Mus musculus troponin I, skeletal, slow 1 (Tnni1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu23491	XM_011247974.3 Latest version!	Mus musculus troponin I, skeletal, slow 1 (Tnni1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$69.30 $99.00
View Old Accession Versions >>
OMu23491	XM_011247974.2	Mus musculus troponin I, skeletal, slow 1 (Tnni1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tnni1 ( NM_001112702.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001106173.1 Mus musculus troponin I, skeletal, slow 1 (Tnni1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu23491
Clone ID Related Accession (Same CDS sequence)	XM_006529382.3 , NM_001112702.1 , XM_011247974.3 , XM_011247974.2 , NM_021467.5
Accession Version	NM_001112702.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 564bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Mus musculus(house mouse)
Product	troponin I, slow skeletal muscle
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AA271187.1, AK012258.1, CR521220.1 and AV124377.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AA271187.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164134, SAMN01164137 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCGGAAG TTGAGAGGAA ATCCAAGATC ACTGCCTCCC GTAAACTCAT GCTGAAGAGC 
CTGATGCTAG CCAAGGCCAA GGAGTGTTGG GAGCAGGAAC ACGAGGAGCG AGAGGCTGAG 
AAGGTGCGTT ACCTCTCGGA GCGCATTCCC ACACTGCAGA CCCGCGGTCT GTCTCTCAGT 
GCCCTTCAGG ACTTGTGCCG AGAGCTCCAT GCCAAGGTAG AGGTGGTGGA TGAGGAGCGC 
TATGATATTG AAGCCAAATG CCTCCACAAC ACCAGAGAGA TCAAGGACCT GAAGCTGAAG 
GTGCTGGACC TCCGTGGGAA GTTCAAGCGT CCTCCCCTGC GCCGGGTCCG TGTCTCAGCC 
GATGCCATGC TCCGAGCCCT ACTGGGCTCT AAGCACAAGG TGTCCATGGA TCTGCGGGCC 
AACCTCAAGT CTGTGAAGAA AGAAGACACA GAAAAGGAGC GGCCAGTCGA GGTAGGAGAC 
TGGAGGAAGA ATGTGGAGGC TATGTCTGGC ATGGAAGGCC GCAAGAAGAT GTTCGACGCT 
GCTAAGTCCC CGACCTCCCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001106173.1
CDS	96..659
Translation	MPEVERKSKITASRKLMLKSLMLAKAKECWEQEHEEREAEKVRYLSERIPTLQTRGLSLSALQDLCRELHAKVEVVDEERYDIEAKCLHNTREIKDLKLKVLDLRGKFKRPPLRRVRVSADAMLRALLGSKHKVSMDLRANLKSVKKEDTEKERPVEVGDWRKNVEAMSGMEGRKKMFDAAKSPTSQ

Target ORF information:

RefSeq Version	NM_001112702.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus troponin I, skeletal, slow 1 (Tnni1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001112702.1

ORF Insert Sequence:

ATGCCGGAAG TTGAGAGGAA ATCCAAGATC ACTGCCTCCC GTAAACTCAT GCTGAAGAGC 
CTGATGCTAG CCAAGGCCAA GGAGTGTTGG GAGCAGGAAC ACGAGGAGCG AGAGGCTGAG 
AAGGTGCGTT ACCTCTCGGA GCGCATTCCC ACACTGCAGA CCCGCGGTCT GTCTCTCAGT 
GCCCTTCAGG ACTTGTGCCG AGAGCTCCAT GCCAAGGTAG AGGTGGTGGA TGAGGAGCGC 
TATGATATTG AAGCCAAATG CCTCCACAAC ACCAGAGAGA TCAAGGACCT GAAGCTGAAG 
GTGCTGGACC TCCGTGGGAA GTTCAAGCGT CCTCCCCTGC GCCGGGTCCG TGTCTCAGCC 
GATGCCATGC TCCGAGCCCT ACTGGGCTCT AAGCACAAGG TGTCCATGGA TCTGCGGGCC 
AACCTCAAGT CTGTGAAGAA AGAAGACACA GAAAAGGAGC GGCCAGTCGA GGTAGGAGAC 
TGGAGGAAGA ATGTGGAGGC TATGTCTGGC ATGGAAGGCC GCAAGAAGAT GTTCGACGCT 
GCTAAGTCCC CGACCTCCCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tnni1 ( NM_021467.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_067442.1 Mus musculus troponin I, skeletal, slow 1 (Tnni1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu23491
Clone ID Related Accession (Same CDS sequence)	XM_006529382.3 , NM_001112702.1 , XM_011247974.3 , XM_011247974.2 , NM_021467.5
Accession Version	NM_021467.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 564bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Mus musculus(house mouse)
Product	troponin I, slow skeletal muscle
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AA230582.1, AK012258.1, CR521220.1 and AV124377.1. On Dec 18, 2007 this sequence version replaced NM_021467.4. Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK012258.1, BY711428.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCGGAAG TTGAGAGGAA ATCCAAGATC ACTGCCTCCC GTAAACTCAT GCTGAAGAGC 
CTGATGCTAG CCAAGGCCAA GGAGTGTTGG GAGCAGGAAC ACGAGGAGCG AGAGGCTGAG 
AAGGTGCGTT ACCTCTCGGA GCGCATTCCC ACACTGCAGA CCCGCGGTCT GTCTCTCAGT 
GCCCTTCAGG ACTTGTGCCG AGAGCTCCAT GCCAAGGTAG AGGTGGTGGA TGAGGAGCGC 
TATGATATTG AAGCCAAATG CCTCCACAAC ACCAGAGAGA TCAAGGACCT GAAGCTGAAG 
GTGCTGGACC TCCGTGGGAA GTTCAAGCGT CCTCCCCTGC GCCGGGTCCG TGTCTCAGCC 
GATGCCATGC TCCGAGCCCT ACTGGGCTCT AAGCACAAGG TGTCCATGGA TCTGCGGGCC 
AACCTCAAGT CTGTGAAGAA AGAAGACACA GAAAAGGAGC GGCCAGTCGA GGTAGGAGAC 
TGGAGGAAGA ATGTGGAGGC TATGTCTGGC ATGGAAGGCC GCAAGAAGAT GTTCGACGCT 
GCTAAGTCCC CGACCTCCCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_067442.1
CDS	144..707
Translation	MPEVERKSKITASRKLMLKSLMLAKAKECWEQEHEEREAEKVRYLSERIPTLQTRGLSLSALQDLCRELHAKVEVVDEERYDIEAKCLHNTREIKDLKLKVLDLRGKFKRPPLRRVRVSADAMLRALLGSKHKVSMDLRANLKSVKKEDTEKERPVEVGDWRKNVEAMSGMEGRKKMFDAAKSPTSQ

Target ORF information:

RefSeq Version	NM_021467.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus troponin I, skeletal, slow 1 (Tnni1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021467.5

ORF Insert Sequence:

ATGCCGGAAG TTGAGAGGAA ATCCAAGATC ACTGCCTCCC GTAAACTCAT GCTGAAGAGC 
CTGATGCTAG CCAAGGCCAA GGAGTGTTGG GAGCAGGAAC ACGAGGAGCG AGAGGCTGAG 
AAGGTGCGTT ACCTCTCGGA GCGCATTCCC ACACTGCAGA CCCGCGGTCT GTCTCTCAGT 
GCCCTTCAGG ACTTGTGCCG AGAGCTCCAT GCCAAGGTAG AGGTGGTGGA TGAGGAGCGC 
TATGATATTG AAGCCAAATG CCTCCACAAC ACCAGAGAGA TCAAGGACCT GAAGCTGAAG 
GTGCTGGACC TCCGTGGGAA GTTCAAGCGT CCTCCCCTGC GCCGGGTCCG TGTCTCAGCC 
GATGCCATGC TCCGAGCCCT ACTGGGCTCT AAGCACAAGG TGTCCATGGA TCTGCGGGCC 
AACCTCAAGT CTGTGAAGAA AGAAGACACA GAAAAGGAGC GGCCAGTCGA GGTAGGAGAC 
TGGAGGAAGA ATGTGGAGGC TATGTCTGGC ATGGAAGGCC GCAAGAAGAT GTTCGACGCT 
GCTAAGTCCC CGACCTCCCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tnni1 ( XM_006529382.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006529445.1 Mus musculus troponin I, skeletal, slow 1 (Tnni1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu23491
Clone ID Related Accession (Same CDS sequence)	XM_006529382.3 , NM_001112702.1 , XM_011247974.3 , XM_011247974.2 , NM_021467.5
Accession Version	XM_006529382.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 564bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	troponin I, slow skeletal muscle isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006529382.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGAAG TTGAGAGGAA ATCCAAGATC ACTGCCTCCC GTAAACTCAT GCTGAAGAGC 
CTGATGCTAG CCAAGGCCAA GGAGTGTTGG GAGCAGGAAC ACGAGGAGCG AGAGGCTGAG 
AAGGTGCGTT ACCTCTCGGA GCGCATTCCC ACACTGCAGA CCCGCGGTCT GTCTCTCAGT 
GCCCTTCAGG ACTTGTGCCG AGAGCTCCAT GCCAAGGTAG AGGTGGTGGA TGAGGAGCGC 
TATGATATTG AAGCCAAATG CCTCCACAAC ACCAGAGAGA TCAAGGACCT GAAGCTGAAG 
GTGCTGGACC TCCGTGGGAA GTTCAAGCGT CCTCCCCTGC GCCGGGTCCG TGTCTCAGCC 
GATGCCATGC TCCGAGCCCT ACTGGGCTCT AAGCACAAGG TGTCCATGGA TCTGCGGGCC 
AACCTCAAGT CTGTGAAGAA AGAAGACACA GAAAAGGAGC GGCCAGTCGA GGTAGGAGAC 
TGGAGGAAGA ATGTGGAGGC TATGTCTGGC ATGGAAGGCC GCAAGAAGAT GTTCGACGCT 
GCTAAGTCCC CGACCTCCCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006529445.1
CDS	361..924
Translation	MPEVERKSKITASRKLMLKSLMLAKAKECWEQEHEEREAEKVRYLSERIPTLQTRGLSLSALQDLCRELHAKVEVVDEERYDIEAKCLHNTREIKDLKLKVLDLRGKFKRPPLRRVRVSADAMLRALLGSKHKVSMDLRANLKSVKKEDTEKERPVEVGDWRKNVEAMSGMEGRKKMFDAAKSPTSQ

Target ORF information:

RefSeq Version	XM_006529382.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus troponin I, skeletal, slow 1 (Tnni1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006529382.3

ORF Insert Sequence:

ATGCCGGAAG TTGAGAGGAA ATCCAAGATC ACTGCCTCCC GTAAACTCAT GCTGAAGAGC 
CTGATGCTAG CCAAGGCCAA GGAGTGTTGG GAGCAGGAAC ACGAGGAGCG AGAGGCTGAG 
AAGGTGCGTT ACCTCTCGGA GCGCATTCCC ACACTGCAGA CCCGCGGTCT GTCTCTCAGT 
GCCCTTCAGG ACTTGTGCCG AGAGCTCCAT GCCAAGGTAG AGGTGGTGGA TGAGGAGCGC 
TATGATATTG AAGCCAAATG CCTCCACAAC ACCAGAGAGA TCAAGGACCT GAAGCTGAAG 
GTGCTGGACC TCCGTGGGAA GTTCAAGCGT CCTCCCCTGC GCCGGGTCCG TGTCTCAGCC 
GATGCCATGC TCCGAGCCCT ACTGGGCTCT AAGCACAAGG TGTCCATGGA TCTGCGGGCC 
AACCTCAAGT CTGTGAAGAA AGAAGACACA GAAAAGGAGC GGCCAGTCGA GGTAGGAGAC 
TGGAGGAAGA ATGTGGAGGC TATGTCTGGC ATGGAAGGCC GCAAGAAGAT GTTCGACGCT 
GCTAAGTCCC CGACCTCCCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tnni1 ( XM_011247974.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246276.1 Mus musculus troponin I, skeletal, slow 1 (Tnni1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu23491
Clone ID Related Accession (Same CDS sequence)	XM_006529382.3 , NM_001112702.1 , XM_011247974.3 , XM_011247974.2 , NM_021467.5
Accession Version	XM_011247974.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 564bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	troponin I, slow skeletal muscle isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011247974.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGAAG TTGAGAGGAA ATCCAAGATC ACTGCCTCCC GTAAACTCAT GCTGAAGAGC 
CTGATGCTAG CCAAGGCCAA GGAGTGTTGG GAGCAGGAAC ACGAGGAGCG AGAGGCTGAG 
AAGGTGCGTT ACCTCTCGGA GCGCATTCCC ACACTGCAGA CCCGCGGTCT GTCTCTCAGT 
GCCCTTCAGG ACTTGTGCCG AGAGCTCCAT GCCAAGGTAG AGGTGGTGGA TGAGGAGCGC 
TATGATATTG AAGCCAAATG CCTCCACAAC ACCAGAGAGA TCAAGGACCT GAAGCTGAAG 
GTGCTGGACC TCCGTGGGAA GTTCAAGCGT CCTCCCCTGC GCCGGGTCCG TGTCTCAGCC 
GATGCCATGC TCCGAGCCCT ACTGGGCTCT AAGCACAAGG TGTCCATGGA TCTGCGGGCC 
AACCTCAAGT CTGTGAAGAA AGAAGACACA GAAAAGGAGC GGCCAGTCGA GGTAGGAGAC 
TGGAGGAAGA ATGTGGAGGC TATGTCTGGC ATGGAAGGCC GCAAGAAGAT GTTCGACGCT 
GCTAAGTCCC CGACCTCCCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246276.1
CDS	457..1020
Translation	MPEVERKSKITASRKLMLKSLMLAKAKECWEQEHEEREAEKVRYLSERIPTLQTRGLSLSALQDLCRELHAKVEVVDEERYDIEAKCLHNTREIKDLKLKVLDLRGKFKRPPLRRVRVSADAMLRALLGSKHKVSMDLRANLKSVKKEDTEKERPVEVGDWRKNVEAMSGMEGRKKMFDAAKSPTSQ

Target ORF information:

RefSeq Version	XM_011247974.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus troponin I, skeletal, slow 1 (Tnni1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011247974.2

ORF Insert Sequence:

ATGCCGGAAG TTGAGAGGAA ATCCAAGATC ACTGCCTCCC GTAAACTCAT GCTGAAGAGC 
CTGATGCTAG CCAAGGCCAA GGAGTGTTGG GAGCAGGAAC ACGAGGAGCG AGAGGCTGAG 
AAGGTGCGTT ACCTCTCGGA GCGCATTCCC ACACTGCAGA CCCGCGGTCT GTCTCTCAGT 
GCCCTTCAGG ACTTGTGCCG AGAGCTCCAT GCCAAGGTAG AGGTGGTGGA TGAGGAGCGC 
TATGATATTG AAGCCAAATG CCTCCACAAC ACCAGAGAGA TCAAGGACCT GAAGCTGAAG 
GTGCTGGACC TCCGTGGGAA GTTCAAGCGT CCTCCCCTGC GCCGGGTCCG TGTCTCAGCC 
GATGCCATGC TCCGAGCCCT ACTGGGCTCT AAGCACAAGG TGTCCATGGA TCTGCGGGCC 
AACCTCAAGT CTGTGAAGAA AGAAGACACA GAAAAGGAGC GGCCAGTCGA GGTAGGAGAC 
TGGAGGAAGA ATGTGGAGGC TATGTCTGGC ATGGAAGGCC GCAAGAAGAT GTTCGACGCT 
GCTAAGTCCC CGACCTCCCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tnni1 ( XM_011247974.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011246276.1 Mus musculus troponin I, skeletal, slow 1 (Tnni1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu23491
Clone ID Related Accession (Same CDS sequence)	XM_006529382.3 , NM_001112702.1 , XM_011247974.3 , XM_011247974.2 , NM_021467.5
Accession Version	XM_011247974.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 564bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	troponin I, slow skeletal muscle isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000067.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_011247974.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGAAG TTGAGAGGAA ATCCAAGATC ACTGCCTCCC GTAAACTCAT GCTGAAGAGC 
CTGATGCTAG CCAAGGCCAA GGAGTGTTGG GAGCAGGAAC ACGAGGAGCG AGAGGCTGAG 
AAGGTGCGTT ACCTCTCGGA GCGCATTCCC ACACTGCAGA CCCGCGGTCT GTCTCTCAGT 
GCCCTTCAGG ACTTGTGCCG AGAGCTCCAT GCCAAGGTAG AGGTGGTGGA TGAGGAGCGC 
TATGATATTG AAGCCAAATG CCTCCACAAC ACCAGAGAGA TCAAGGACCT GAAGCTGAAG 
GTGCTGGACC TCCGTGGGAA GTTCAAGCGT CCTCCCCTGC GCCGGGTCCG TGTCTCAGCC 
GATGCCATGC TCCGAGCCCT ACTGGGCTCT AAGCACAAGG TGTCCATGGA TCTGCGGGCC 
AACCTCAAGT CTGTGAAGAA AGAAGACACA GAAAAGGAGC GGCCAGTCGA GGTAGGAGAC 
TGGAGGAAGA ATGTGGAGGC TATGTCTGGC ATGGAAGGCC GCAAGAAGAT GTTCGACGCT 
GCTAAGTCCC CGACCTCCCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011246276.1
CDS	456..1019
Translation	MPEVERKSKITASRKLMLKSLMLAKAKECWEQEHEEREAEKVRYLSERIPTLQTRGLSLSALQDLCRELHAKVEVVDEERYDIEAKCLHNTREIKDLKLKVLDLRGKFKRPPLRRVRVSADAMLRALLGSKHKVSMDLRANLKSVKKEDTEKERPVEVGDWRKNVEAMSGMEGRKKMFDAAKSPTSQ

Target ORF information:

RefSeq Version	XM_011247974.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus troponin I, skeletal, slow 1 (Tnni1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011247974.3

ORF Insert Sequence:

ATGCCGGAAG TTGAGAGGAA ATCCAAGATC ACTGCCTCCC GTAAACTCAT GCTGAAGAGC 
CTGATGCTAG CCAAGGCCAA GGAGTGTTGG GAGCAGGAAC ACGAGGAGCG AGAGGCTGAG 
AAGGTGCGTT ACCTCTCGGA GCGCATTCCC ACACTGCAGA CCCGCGGTCT GTCTCTCAGT 
GCCCTTCAGG ACTTGTGCCG AGAGCTCCAT GCCAAGGTAG AGGTGGTGGA TGAGGAGCGC 
TATGATATTG AAGCCAAATG CCTCCACAAC ACCAGAGAGA TCAAGGACCT GAAGCTGAAG 
GTGCTGGACC TCCGTGGGAA GTTCAAGCGT CCTCCCCTGC GCCGGGTCCG TGTCTCAGCC 
GATGCCATGC TCCGAGCCCT ACTGGGCTCT AAGCACAAGG TGTCCATGGA TCTGCGGGCC 
AACCTCAAGT CTGTGAAGAA AGAAGACACA GAAAAGGAGC GGCCAGTCGA GGTAGGAGAC 
TGGAGGAAGA ATGTGGAGGC TATGTCTGGC ATGGAAGGCC GCAAGAAGAT GTTCGACGCT 
GCTAAGTCCC CGACCTCCCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Inactivation of the myogenic bHLH gene MRF4 results in up-regulation of myogenin and rib anomalies.
Genes & development9(11)1388-99(1995 Jun)
Zhang W,Behringer RR,Olson EN

Transcriptional enhancer factor 1 disruption by a retroviral gene trap leads to heart defects and embryonic lethality in mice.
Genes & development8(19)2293-301(1994 Oct)
Chen Z,Friedrich GA,Soriano P

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Tnni1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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