TNNI1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TNNI1
Entrez Gene ID	7135
Full Name	troponin I1, slow skeletal type
Synonyms	SSTNI,TNN1
General protein information	Preferred Names troponin I1, slow skeletal type Names troponin I, slow skeletal muscle troponin I type 1 (skeletal, slow) troponin I, slow-twitch isoform
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1q32.1
Summary	Troponin proteins associate with tropomyosin and regulate the calcium sensitivity of the myofibril contractile apparatus of striated muscles. Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. The TnI-fast and TnI-slow genes are expressed in fast-twitch and slow-twitch skeletal muscle fibers, respectively, while the TnI-cardiac gene is expressed exclusively in cardiac muscle tissue. This gene encodes the Troponin-I-skeletal-slow-twitch protein. This gene is expressed in cardiac and skeletal muscle during early development but is restricted to slow-twitch skeletal muscle fibers in adults. The encoded protein prevents muscle contraction by inhibiting calcium-mediated conformational changes in actin-myosin complexes. [provided by RefSeq, Jul 2008].
Disorder MIM:	191042

mRNA and Protein(s)

mRNA	Protein	Name
NM_003281.3	NP_003272.3	troponin I, slow skeletal muscle
NM_003281.4	NP_003272.3	troponin I, slow skeletal muscle

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Tnni1	NP_058880.1
Gallus gallus (chicken)	TNNI1	XP_004934896.1
Pan troglodytes (chimpanzee)	TNNI1	XP_525019.2
Canis lupus familiaris (dog)	TNNI1	XP_003639192.1
Mus musculus (house mouse)	Tnni1	NP_001106173.1
Danio rerio (zebrafish)	tnni1b	NP_001008613.1
Homo sapiens (human)	TNNI1	NP_003272.3
	TNNI1	XP_001108591.1
Bos taurus (cattle)	TNNI1	XP_002694161.1
Xenopus tropicalis (tropical clawed frog)	LOC100125108	NP_001096486.1

Gene Ontology
Bibliography

Related articles in PubMed

Ultra-sensitive troponin I is an independent predictor of incident coronary heart disease in the general population.
Kaess BM, de Las Heras Gala T, Zierer A, Meisinger C, Wahl S, Peters A, Todd J, Herder C, Huth C, Thorand B, Koenig W
European journal of epidemiology32(7)583-591(2017 Jul)

Left Ventricular Systolic Dysfunction Predicted By Early Troponin I Release After Anthracycline Based Chemotherapy In Breast Cancer Patients.
Shafi A, Siddiqui N, Imtiaz S, Din Sajid MU
Journal of Ayub Medical College, Abbottabad : JAMC29(2)266-269(2017 Apr-Jun)

Diagnostic Value of Mean Platelet Volume Combined With Troponin I for Acute Coronary Syndrome.
Wang X, Xu XL, Li XM, Zhao R, Yang X, Cong HL
The American journal of the medical sciences352(2)159-65(2016 Aug)

Relations of circulating GDF-15, soluble ST2, and troponin-I concentrations with vascular function in the community: The Framingham Heart Study.
Andersson C, Enserro D, Sullivan L, Wang TJ, Januzzi JL Jr, Benjamin EJ, Vita JA, Hamburg NM, Larson MG, Mitchell GF, Vasan RS
Atherosclerosis248245-51(2016 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Measuring mean platelet volume in conjunction with troponin I levels improves the diagnostic value of troponin I in acute coronary syndrome.
Title: Diagnostic Value of Mean Platelet Volume Combined With Troponin I for Acute Coronary Syndrome.

GDF-15, sST2, and hs-TnI are associated with incident cardiovascular disease and heart failure
Title: Relations of circulating GDF-15, soluble ST2, and troponin-I concentrations with vascular function in the community: The Framingham Heart Study.

CT-1 was found to be associated with Tn-I, which is used to detect myocardial damage after OPCAB surgery. CT-1 may also be used to detect myocardial damage.
Title: The relationship between cardiotrophin-1 and troponin-i in coronary arterial bypass grafting on the beating heart: a prospective study.

Data suggest that mutation A162H in switch region of troponin I induces transitory curved conformation and promotes contraction of troponin I bound to regulatory domain of troponin C; this is countered by residue E164 to ensure proper relaxation.
Title: Structure and Dynamics of the Acidosis-Resistant A162H Mutant of the Switch Region of Troponin I Bound to the Regulatory Domain of Troponin C.

TNNI3K is a cardiac-specific gene, encoding a cardiac troponin I-interacting MAP kinase. In mice, Tnni3k is shown to play an important role in the regulation of cardiac differentiation and cardiac contractility
Title: Whole exome sequencing identifies the TNNI3K gene as a cause of familial conduction system disease and congenital junctional ectopic tachycardia.

The following TNNI1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TNNI1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu18877	NM_003281.4 Latest version!	Homo sapiens troponin I1, slow skeletal type (TNNI1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu18877	NM_003281.3	Homo sapiens troponin I1, slow skeletal type (TNNI1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TNNI1 ( NM_003281.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_003272.3 Homo sapiens troponin I1, slow skeletal type (TNNI1), mRNA.

Price & Availability↑

CloneID	OHu18877
Clone ID Related Accession (Same CDS sequence)	NM_003281.3 , NM_003281.4
Accession Version	NM_003281.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 564bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-30
Organism	Homo sapiens(human)
Product	troponin I, slow skeletal muscle
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI825503.1, BC012600.1, AC096677.2 and AL832006.2. This sequence is a reference standard in the RefSeqGene project. On Dec 16, 2004 this sequence version replaced NM_003281.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: ERR279832.7794.1, AL831975.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2162946, SAMN03267751 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGCCGGAAG TCGAGAGAAA ACCCAAGATC ACTGCCTCCC GCAAACTCTT GCTGAAGAGC 
CTGATGCTGG CCAAGGCCAA GGAATGCTGG GAGCAGGAGC ACGAGGAGCG CGAGGCTGAG 
AAGGTGCGCT ACCTGGCAGA GCGCATCCCC ACGCTGCAGA CCCGTGGCCT GTCCCTCAGT 
GCCCTGCAGG ACCTGTGCCG GGAGCTGCAC GCCAAGGTGG AGGTGGTGGA TGAGGAGCGA 
TACGACATTG AGGCCAAATG CCTCCACAAC ACCAGGGAGA TTAAGGACCT GAAGCTGAAG 
GTGATGGACC TCCGTGGGAA GTTCAAGCGC CCGCCCCTGC GTCGAGTCCG TGTCTCGGCT 
GACGCCATGC TCCGGGCCCT GCTGGGCTCC AAGCACAAGG TGTCCATGGA TCTGCGGGCC 
AACCTCAAGT CTGTGAAGAA GGAAGACACA GAGAAGGAGC GGCCTGTGGA GGTGGGTGAC 
TGGAGGAAGA ACGTGGAGGC CATGTCTGGC ATGGAAGGCC GGAAGAAGAT GTTTGATGCC 
GCCAAGTCTC CGACCTCACA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003272.3
CDS	94..657
Translation	MPEVERKPKITASRKLLLKSLMLAKAKECWEQEHEEREAEKVRYLAERIPTLQTRGLSLSALQDLCRELHAKVEVVDEERYDIEAKCLHNTREIKDLKLKVMDLRGKFKRPPLRRVRVSADAMLRALLGSKHKVSMDLRANLKSVKKEDTEKERPVEVGDWRKNVEAMSGMEGRKKMFDAAKSPTSQ

Target ORF information:

RefSeq Version	NM_003281.3
Organism	Homo sapiens(human)
Definition	Homo sapiens troponin I1, slow skeletal type (TNNI1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003281.3

ORF Insert Sequence:

ATGCCGGAAG TCGAGAGAAA ACCCAAGATC ACTGCCTCCC GCAAACTCTT GCTGAAGAGC 
CTGATGCTGG CCAAGGCCAA GGAATGCTGG GAGCAGGAGC ACGAGGAGCG CGAGGCTGAG 
AAGGTGCGCT ACCTGGCAGA GCGCATCCCC ACGCTGCAGA CCCGTGGCCT GTCCCTCAGT 
GCCCTGCAGG ACCTGTGCCG GGAGCTGCAC GCCAAGGTGG AGGTGGTGGA TGAGGAGCGA 
TACGACATTG AGGCCAAATG CCTCCACAAC ACCAGGGAGA TTAAGGACCT GAAGCTGAAG 
GTGATGGACC TCCGTGGGAA GTTCAAGCGC CCGCCCCTGC GTCGAGTCCG TGTCTCGGCT 
GACGCCATGC TCCGGGCCCT GCTGGGCTCC AAGCACAAGG TGTCCATGGA TCTGCGGGCC 
AACCTCAAGT CTGTGAAGAA GGAAGACACA GAGAAGGAGC GGCCTGTGGA GGTGGGTGAC 
TGGAGGAAGA ACGTGGAGGC CATGTCTGGC ATGGAAGGCC GGAAGAAGAT GTTTGATGCC 
GCCAAGTCTC CGACCTCACA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNNI1 ( NM_003281.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_003272.3 Homo sapiens troponin I1, slow skeletal type (TNNI1), mRNA.

Price & Availability↑

CloneID	OHu18877
Clone ID Related Accession (Same CDS sequence)	NM_003281.3 , NM_003281.4
Accession Version	NM_003281.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 564bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Homo sapiens(human)
Product	troponin I, slow skeletal muscle
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC012600.1, AC096677.2 and AL832006.2. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_003281.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AL831975.1, BX510903.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2162946, SAMN03267751 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000361379.9/ ENSP00000354488.4 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCCGGAAG TCGAGAGAAA ACCCAAGATC ACTGCCTCCC GCAAACTCTT GCTGAAGAGC 
CTGATGCTGG CCAAGGCCAA GGAATGCTGG GAGCAGGAGC ACGAGGAGCG CGAGGCTGAG 
AAGGTGCGCT ACCTGGCAGA GCGCATCCCC ACGCTGCAGA CCCGTGGCCT GTCCCTCAGT 
GCCCTGCAGG ACCTGTGCCG GGAGCTGCAC GCCAAGGTGG AGGTGGTGGA TGAGGAGCGA 
TACGACATTG AGGCCAAATG CCTCCACAAC ACCAGGGAGA TTAAGGACCT GAAGCTGAAG 
GTGATGGACC TCCGTGGGAA GTTCAAGCGC CCGCCCCTGC GTCGAGTCCG TGTCTCGGCT 
GACGCCATGC TCCGGGCCCT GCTGGGCTCC AAGCACAAGG TGTCCATGGA TCTGCGGGCC 
AACCTCAAGT CTGTGAAGAA GGAAGACACA GAGAAGGAGC GGCCTGTGGA GGTGGGTGAC 
TGGAGGAAGA ACGTGGAGGC CATGTCTGGC ATGGAAGGCC GGAAGAAGAT GTTTGATGCC 
GCCAAGTCTC CGACCTCACA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003272.3
CDS	78..641
Translation	MPEVERKPKITASRKLLLKSLMLAKAKECWEQEHEEREAEKVRYLAERIPTLQTRGLSLSALQDLCRELHAKVEVVDEERYDIEAKCLHNTREIKDLKLKVMDLRGKFKRPPLRRVRVSADAMLRALLGSKHKVSMDLRANLKSVKKEDTEKERPVEVGDWRKNVEAMSGMEGRKKMFDAAKSPTSQ

Target ORF information:

RefSeq Version	NM_003281.4
Organism	Homo sapiens(human)
Definition	Homo sapiens troponin I1, slow skeletal type (TNNI1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003281.4

ORF Insert Sequence:

ATGCCGGAAG TCGAGAGAAA ACCCAAGATC ACTGCCTCCC GCAAACTCTT GCTGAAGAGC 
CTGATGCTGG CCAAGGCCAA GGAATGCTGG GAGCAGGAGC ACGAGGAGCG CGAGGCTGAG 
AAGGTGCGCT ACCTGGCAGA GCGCATCCCC ACGCTGCAGA CCCGTGGCCT GTCCCTCAGT 
GCCCTGCAGG ACCTGTGCCG GGAGCTGCAC GCCAAGGTGG AGGTGGTGGA TGAGGAGCGA 
TACGACATTG AGGCCAAATG CCTCCACAAC ACCAGGGAGA TTAAGGACCT GAAGCTGAAG 
GTGATGGACC TCCGTGGGAA GTTCAAGCGC CCGCCCCTGC GTCGAGTCCG TGTCTCGGCT 
GACGCCATGC TCCGGGCCCT GCTGGGCTCC AAGCACAAGG TGTCCATGGA TCTGCGGGCC 
AACCTCAAGT CTGTGAAGAA GGAAGACACA GAGAAGGAGC GGCCTGTGGA GGTGGGTGAC 
TGGAGGAAGA ACGTGGAGGC CATGTCTGGC ATGGAAGGCC GGAAGAAGAT GTTTGATGCC 
GCCAAGTCTC CGACCTCACA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Ultra-sensitive troponin I is an independent predictor of incident coronary heart disease in the general population.
European journal of epidemiology32(7)583-591(2017 Jul)
Kaess BM,de Las Heras Gala T,Zierer A,Meisinger C,Wahl S,Peters A,Todd J,Herder C,Huth C,Thorand B,Koenig W

Left Ventricular Systolic Dysfunction Predicted By Early Troponin I Release After Anthracycline Based Chemotherapy In Breast Cancer Patients.
Journal of Ayub Medical College, Abbottabad : JAMC29(2)266-269(2017 Apr-Jun)
Shafi A,Siddiqui N,Imtiaz S,Din Sajid MU

Diagnostic Value of Mean Platelet Volume Combined With Troponin I for Acute Coronary Syndrome.
The American journal of the medical sciences352(2)159-65(2016 Aug)
Wang X,Xu XL,Li XM,Zhao R,Yang X,Cong HL

Relations of circulating GDF-15, soluble ST2, and troponin-I concentrations with vascular function in the community: The Framingham Heart Study.
Atherosclerosis248245-51(2016 May)
Andersson C,Enserro D,Sullivan L,Wang TJ,Januzzi JL Jr,Benjamin EJ,Vita JA,Hamburg NM,Larson MG,Mitchell GF,Vasan RS

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TNNI1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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