F2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	F2
Entrez Gene ID	29251
Full Name	coagulation factor II
General protein information	Preferred Names coagulation factor II Names prothrombin coagulation factor 2 thrombin
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3q24
Summary	catalyzes the preferential cleavage of Arg-Gly; activates fibrinogen to fibrin and releases fibrinopeptides A and B; involved in blood coagulation and wound repair [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_022924.2	NP_075213.2	prothrombin precursor

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	F2	NP_075213.2
Danio rerio (zebrafish)	f2	NP_998555.1
Gallus gallus (chicken)	F2	NP_989936.1
Homo sapiens (human)	F2	NP_000497.1
	F2	NP_001098055.1
Canis lupus familiaris (dog)	F2	XP_003639742.1
Bos taurus (cattle)	F2	NP_776302.1
Xenopus tropicalis (tropical clawed frog)	f2	NP_001015797.1
Pan troglodytes (chimpanzee)	F2	XP_001165233.1
Mus musculus (house mouse)	F2	NP_034298.1

Gene Ontology
Bibliography

Related articles in PubMed

The role of spinal thrombin through protease-activated receptor 1 in hyperalgesia after neural injury.
Smith JR, Winkelstein BA
Journal of neurosurgery. Spine26(4)532-541(2017 Apr)

Serum omentin and vaspin levels in cirrhotic patients with and without portal vein thrombosis.
Kukla M, Waluga M, ?orniak M, Berdowska A, Wosiewicz P, Sawczyn T, Bu?dak RJ, Ochman M, Ziora K, Krzemi?ski T, Hartleb M
World journal of gastroenterology23(14)2613-2624(2017 Apr)

Contribution of thrombin-reactive brain pericytes to blood-brain barrier dysfunction in an in vivo mouse model of obesity-associated diabetes and an in vitro rat model.
Machida T, Takata F, Matsumoto J, Miyamura T, Hirata R, Kimura I, Kataoka Y, Dohgu S, Yamauchi A
PloS one12(5)e0177447(2017)

Cross Talk Pathways Between Coagulation and Inflammation.
Foley JH, Conway EM
Circulation research118(9)1392-408(2016 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study suggests a potent role for spinal thrombin and its activation of PAR1 in pain onset following neuropathic injury
Title: The role of spinal thrombin through protease-activated receptor 1 in hyperalgesia after neural injury.

These findings imply that a thrombin-pericyte interaction in the brain could be a significant mechanism responsible for blood-brain barrier dysfunction under obesity-associated diabetic conditions and thus could represent a therapeutic target for central nervous system complications of diabetes.
Title: Contribution of thrombin-reactive brain pericytes to blood-brain barrier dysfunction in an in vivo mouse model of obesity-associated diabetes and an in vitro rat model.

Inflammatory bowel disease is associated with production of prothrombin in the dorsal motor nucleus of the vagus (DMV). Thrombin in the DMV causes vagal neuron apoptosis and decreased sensitivity to neurotransmitters.
Title: Thrombin mediates vagal apoptosis and dysfunction in inflammatory bowel disease.

Thrombin-induced overexpression of TGF-beta1 and activation of its downstream factors might be a mechanism of communicating hydrocephalus after subarachnoid hemorrhage.
Title: Thrombin-induced TGF-?1 pathway: a cause of communicating hydrocephalus post subarachnoid hemorrhage.

These results suggest that thrombin triggers the generation of epileptic seizures by reducing the inhibitory and increasing the excitatory tone in CA3 neurons
Title: Thrombin regulation of synaptic transmission: implications for seizure onset.

The following F2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the F2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa06427	NM_022924.2 Latest version!	Rattus norvegicus coagulation factor II (F2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

F2 ( NM_022924.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_075213.2 Rattus norvegicus coagulation factor II (F2), mRNA.

Price & Availability↑

CloneID	ORa06427
Clone ID Related Accession (Same CDS sequence)	NM_022924.2
Accession Version	NM_022924.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1854bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Rattus norvegicus(Norway rat)
Product	prothrombin precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from EX492416.1, DY318887.1, DW392486.1, DW391550.1 and CO575133.1. On Nov 29, 2007 this sequence version replaced NM_022924.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X52835.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906354, SAMN01906355 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCGCACG TCCGCGGCCT GGGCCTACCT GGCTGCCTGG CTCTGGCTGC CCTGGCTAGC 
CTGGTACACA GTCAGCATGT GTTCCTGGCC CCTCAGCAAG CACTGTCGCT GCTCCAGCGG 
GTCCGCAGAG CCAACAGCGG CTTCCTGGAG GAGCTCCGAA AGGGCAACCT GGAGCGTGAG 
TGCGTGGAAG AGCAGTGCAG CTATGAGGAG GCTTTTGAGG CCCTGGAGTC TCCCCAAGAC 
ACGGATGTGT TCTGGGCCAA GTACACAGTG TGTGATTCAG TGAGGAAACC TCGAGAGACC 
TTCATGGACT GTCTAGAAGG TCGCTGTGCT ATGGATCTGG GTCTGAACTA CCATGGGAAT 
GTGAGTGTCA CCCATACCGG CATCGAGTGT CAGCTGTGGC GAAGCCGCTA CCCACACAGG 
CCTGACATCA ACTCCACCAC CCATCCTGGG GCTGACCTGA AGGAGAACTT CTGCCGAAAT 
CCAGACAGCA GCACCTCAGG GCCCTGGTGC TACACCACAG ACCCTACTGT GCGCAGAGAG 
GAATGCAGTA TCCCTGTCTG TGGTCAGGAG GGCCGTACCA CTGTGAAAAT GACTCCTCGC 
TCAAGAGGTT CCAAGGAGAA TCTGTCGCCT CCATTGGGAG AGTGCCTCCT CGAGCGTGGC 
CGGCTGTACC AGGGGAACTT AGCTGTGACA ACACTTGGGT CCCCCTGCCT GGCCTGGGAC 
AGTTTGCCAA CCAAGACCCT GAGCAAGTAC CAGAACTTCG ACCCTGAGGT GAAACTCGTG 
CAAAACTTCT GCCGCAATCC AGACCGGGAT GAGGAGGGCG CGTGGTGCTT TGTTGCGCAG 
CAGCCTGGTT TTGAATACTG CAGCCTCAAC TACTGTGATG AAGCGGTGGG AGAGGAGAAC 
CATGACGGGG ACGAGTCCAT CGCAGGACGC ACCACCGACG CGGAGTTCCA CACCTTCTTC 
GATGAGAGGA CTTTCGGCCT TGGGGAGGCA GACTGTGGCC TGCGGCCTTT GTTCGAGAAG 
AAGTCCTTGA CAGACAAAAC GGAAAAGGAG CTTCTTGATT CTTACATAGA TGGGCGCATC 
GTGGAGGGCT GGGACGCTGA GAAGGGTATC GCCCCATGGC AGGTGATGCT TTTTCGGAAG 
AGTCCCCAAG AGCTGCTGTG TGGAGCCAGC CTTATCAGTG ATCGATGGGT CCTCACTGCT 
GCCCACTGCA TTCTGTACCC ACCCTGGGAC AAGAACTTCA CTGAGAATGA CCTCCTGGTG 
CGCATTGGCA AGCACTCCAG AACCAGATAT GAGCGGAATG TTGAAAAGAT CTCCATGCTG 
GAAAAGATCT ACATCCACCC CAGATATAAC TGGCGGGAAA ACCTAGACCG TGACATTGCT 
CTGCTCAAGC TAAAGAAGCC AGTGCCCTTC AGTGACTACA TTCACCCCGT GTGCTTGCCA 
GACAAGCAGA CAGTAACCAG TTTGCTCCAG GCTGGTTATA AAGGGCGGGT GACAGGCTGG 
GGCAACCTTC GGGAGACGTG GACAACTAAC ATCAACGAGA TACAGCCCAG CGTCCTGCAG 
GTGGTGAACC TGCCCATTGT AGAGCGACCA GTGTGCAAGG CCTCTACCCG GATACGCATT 
ACTGACAACA TGTTCTGTGC TGGCTTCAAG GTGAATGACA CCAAGCGAGG AGATGCTTGT 
GAAGGTGACA GTGGGGGACC TTTTGTCATG AAGAGCCCCT ACAACCACCG CTGGTACCAA 
ATGGGTATTG TCTCCTGGGG TGAAGGCTGT GACCGGAATG GGAAATATGG CTTCTACACG 
CACGTGTTCC GTCTGAAAAG GTGGATGCAG AAAGTCATTG ATCAGCATAG ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_075213.2
CDS	16..1869
Translation	MSHVRGLGLPGCLALAALASLVHSQHVFLAPQQALSLLQRVRRANSGFLEELRKGNLERECVEEQCSYEEAFEALESPQDTDVFWAKYTVCDSVRKPRETFMDCLEGRCAMDLGLNYHGNVSVTHTGIECQLWRSRYPHRPDINSTTHPGADLKENFCRNPDSSTSGPWCYTTDPTVRREECSIPVCGQEGRTTVKMTPRSRGSKENLSPPLGECLLERGRLYQGNLAVTTLGSPCLAWDSLPTKTLSKYQNFDPEVKLVQNFCRNPDRDEEGAWCFVAQQPGFEYCSLNYCDEAVGEENHDGDESIAGRTTDAEFHTFFDERTFGLGEADCGLRPLFEKKSLTDKTEKELLDSYIDGRIVEGWDAEKGIAPWQVMLFRKSPQELLCGASLISDRWVLTAAHCILYPPWDKNFTENDLLVRIGKHSRTRYERNVEKISMLEKIYIHPRYNWRENLDRDIALLKLKKPVPFSDYIHPVCLPDKQTVTSLLQAGYKGRVTGWGNLRETWTTNINEIQPSVLQVVNLPIVERPVCKASTRIRITDNMFCAGFKVNDTKRGDACEGDSGGPFVMKSPYNHRWYQMGIVSWGEGCDRNGKYGFYTHVFRLKRWMQKVIDQHR

Target ORF information:

RefSeq Version	NM_022924.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus coagulation factor II (F2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022924.2

ORF Insert Sequence:

ATGTCGCACG TCCGCGGCCT GGGCCTACCT GGCTGCCTGG CTCTGGCTGC CCTGGCTAGC 
CTGGTACACA GTCAGCATGT GTTCCTGGCC CCTCAGCAAG CACTGTCGCT GCTCCAGCGG 
GTCCGCAGAG CCAACAGCGG CTTCCTGGAG GAGCTCCGAA AGGGCAACCT GGAGCGTGAG 
TGCGTGGAAG AGCAGTGCAG CTATGAGGAG GCTTTTGAGG CCCTGGAGTC TCCCCAAGAC 
ACGGATGTGT TCTGGGCCAA GTACACAGTG TGTGATTCAG TGAGGAAACC TCGAGAGACC 
TTCATGGACT GTCTAGAAGG TCGCTGTGCT ATGGATCTGG GTCTGAACTA CCATGGGAAT 
GTGAGTGTCA CCCATACCGG CATCGAGTGT CAGCTGTGGC GAAGCCGCTA CCCACACAGG 
CCTGACATCA ACTCCACCAC CCATCCTGGG GCTGACCTGA AGGAGAACTT CTGCCGAAAT 
CCAGACAGCA GCACCTCAGG GCCCTGGTGC TACACCACAG ACCCTACTGT GCGCAGAGAG 
GAATGCAGTA TCCCTGTCTG TGGTCAGGAG GGCCGTACCA CTGTGAAAAT GACTCCTCGC 
TCAAGAGGTT CCAAGGAGAA TCTGTCGCCT CCATTGGGAG AGTGCCTCCT CGAGCGTGGC 
CGGCTGTACC AGGGGAACTT AGCTGTGACA ACACTTGGGT CCCCCTGCCT GGCCTGGGAC 
AGTTTGCCAA CCAAGACCCT GAGCAAGTAC CAGAACTTCG ACCCTGAGGT GAAACTCGTG 
CAAAACTTCT GCCGCAATCC AGACCGGGAT GAGGAGGGCG CGTGGTGCTT TGTTGCGCAG 
CAGCCTGGTT TTGAATACTG CAGCCTCAAC TACTGTGATG AAGCGGTGGG AGAGGAGAAC 
CATGACGGGG ACGAGTCCAT CGCAGGACGC ACCACCGACG CGGAGTTCCA CACCTTCTTC 
GATGAGAGGA CTTTCGGCCT TGGGGAGGCA GACTGTGGCC TGCGGCCTTT GTTCGAGAAG 
AAGTCCTTGA CAGACAAAAC GGAAAAGGAG CTTCTTGATT CTTACATAGA TGGGCGCATC 
GTGGAGGGCT GGGACGCTGA GAAGGGTATC GCCCCATGGC AGGTGATGCT TTTTCGGAAG 
AGTCCCCAAG AGCTGCTGTG TGGAGCCAGC CTTATCAGTG ATCGATGGGT CCTCACTGCT 
GCCCACTGCA TTCTGTACCC ACCCTGGGAC AAGAACTTCA CTGAGAATGA CCTCCTGGTG 
CGCATTGGCA AGCACTCCAG AACCAGATAT GAGCGGAATG TTGAAAAGAT CTCCATGCTG 
GAAAAGATCT ACATCCACCC CAGATATAAC TGGCGGGAAA ACCTAGACCG TGACATTGCT 
CTGCTCAAGC TAAAGAAGCC AGTGCCCTTC AGTGACTACA TTCACCCCGT GTGCTTGCCA 
GACAAGCAGA CAGTAACCAG TTTGCTCCAG GCTGGTTATA AAGGGCGGGT GACAGGCTGG 
GGCAACCTTC GGGAGACGTG GACAACTAAC ATCAACGAGA TACAGCCCAG CGTCCTGCAG 
GTGGTGAACC TGCCCATTGT AGAGCGACCA GTGTGCAAGG CCTCTACCCG GATACGCATT 
ACTGACAACA TGTTCTGTGC TGGCTTCAAG GTGAATGACA CCAAGCGAGG AGATGCTTGT 
GAAGGTGACA GTGGGGGACC TTTTGTCATG AAGAGCCCCT ACAACCACCG CTGGTACCAA 
ATGGGTATTG TCTCCTGGGG TGAAGGCTGT GACCGGAATG GGAAATATGG CTTCTACACG 
CACGTGTTCC GTCTGAAAAG GTGGATGCAG AAAGTCATTG ATCAGCATAG ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The role of spinal thrombin through protease-activated receptor 1 in hyperalgesia after neural injury.
Journal of neurosurgery. Spine26(4)532-541(2017 Apr)
Smith JR,Winkelstein BA

Serum omentin and vaspin levels in cirrhotic patients with and without portal vein thrombosis.
World journal of gastroenterology23(14)2613-2624(2017 Apr)
Kukla M,Waluga M,?orniak M,Berdowska A,Wosiewicz P,Sawczyn T,Bu?dak RJ,Ochman M,Ziora K,Krzemi?ski T,Hartleb M

Contribution of thrombin-reactive brain pericytes to blood-brain barrier dysfunction in an in vivo mouse model of obesity-associated diabetes and an in vitro rat model.
PloS one12(5)e0177447(2017)
Machida T,Takata F,Matsumoto J,Miyamura T,Hirata R,Kimura I,Kataoka Y,Dohgu S,Yamauchi A

Cross Talk Pathways Between Coagulation and Inflammation.
Circulation research118(9)1392-408(2016 Apr)
Foley JH,Conway EM

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F2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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