F2 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	F2
Entrez Gene ID	280685
Full Name	coagulation factor II, thrombin
General protein information	Preferred Names coagulation factor II, thrombin Names prothrombin thrombin
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005216401.3	XP_005216458.1	prothrombin isoform X1
NM_173877.1	NP_776302.1	prothrombin precursor
XM_005216401.4	XP_005216458.2	prothrombin isoform X1
XM_015474857.1	XP_015330343.1	prothrombin isoform X2

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	F2	NP_075213.2
Danio rerio (zebrafish)	f2	NP_998555.1
Gallus gallus (chicken)	F2	NP_989936.1
Homo sapiens (human)	F2	NP_000497.1
	F2	NP_001098055.1
Canis lupus familiaris (dog)	F2	XP_003639742.1
Bos taurus (cattle)	F2	NP_776302.1
Xenopus tropicalis (tropical clawed frog)	f2	NP_001015797.1
Pan troglodytes (chimpanzee)	F2	XP_001165233.1
Mus musculus (house mouse)	F2	NP_034298.1

Gene Ontology
Bibliography

Related articles in PubMed

Kinetic regulation of the binding of prothrombin to phospholipid membranes.
Smith E, Vekaria R, Brown KA, Longstaff C
Molecular and cellular biochemistry382(1-2)193-201(2013 Oct)

Anticoagulant activity of a unique sulfated pyranosic (1->3)-?-L-arabinan through direct interaction with thrombin.
Fern?ndez PV, Quintana I, Cerezo AS, Caramelo JJ, Pol-Fachin L, Verli H, Estevez JM, Ciancia M
The Journal of biological chemistry288(1)223-33(2013 Jan)

Exogenous thrombin delivery promotes collateral capillary arterialization and tissue reperfusion in the murine spinotrapezius muscle ischemia model.
Bruce AC, Peirce SM
Microcirculation (New York, N.Y. : 1994)19(2)143-54(2012 Feb)

Thrombin induces inducible nitric oxide synthase expression via the MAPK, MSK1, and NF-?B signaling pathways in alveolar macrophages.
Lin CC, Shih CH, Yang YL, Bien MY, Lin CH, Yu MC, Sureshbabu M, Chen BC
European journal of pharmacology672(1-3)180-7(2011 Dec)

High glucose enhances thrombin responses via protease-activated receptor-4 in human vascular smooth muscle cells.
Dangwal S, Rauch BH, Gensch T, Dai L, Bretschneider E, Vogelaar CF, Schr?r K, Rosenkranz AC
Arteriosclerosis, thrombosis, and vascular biology31(3)624-33(2011 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the weaker rapid interaction between prothrombin and membranes is the most important in vivo when considering the activation of prothrombin at the cell surface.
Title: Kinetic regulation of the binding of prothrombin to phospholipid membranes.

novel pyranosic sulfated arabinan Ab1 exerts its anticoagulant activity on thrombin by a mechanism different from those found previously for other sulfated polysaccharides and glycosaminoglycans
Title: Anticoagulant activity of a unique sulfated pyranosic (1->3)-?-L-arabinan through direct interaction with thrombin.

Exogenous delivery of thrombin enhances microvascular collateral development in response to ischemic insult, and accelerates tissue reperfusion.
Title: Exogenous thrombin delivery promotes collateral capillary arterialization and tissue reperfusion in the murine spinotrapezius muscle ischemia model.

the signaling pathways of MAPK, MSK1, and NF-kappaB play important roles in thrombin-induced iNOS expression in alveolar macrophages
Title: Thrombin induces inducible nitric oxide synthase expression via the MAPK, MSK1, and NF-?B signaling pathways in alveolar macrophages.

High glucose enhances smooth muscle cell responsiveness to thrombin through transcriptional upregulation of PAR-4, mediated via PKC and NFkappaB.
Title: High glucose enhances thrombin responses via protease-activated receptor-4 in human vascular smooth muscle cells.

The following F2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the F2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa41244	NM_173877.1 Latest version!	Bos taurus coagulation factor II, thrombin (F2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa26705	XM_005216401.3 Latest version!	Bos taurus coagulation factor II, thrombin (F2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa222607	XM_005216401.4 Latest version!	Bos taurus coagulation factor II, thrombin (F2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
OBa92794	XM_015474857.1 Latest version!	Bos taurus coagulation factor II, thrombin (F2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$342.30 $489.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

F2 ( NM_173877.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_776302.1 Bos taurus coagulation factor II, thrombin (F2), mRNA.

Price & Availability↑

CloneID	OBa41244
Clone ID Related Accession (Same CDS sequence)	NM_173877.1
Accession Version	NM_173877.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1878bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Bos taurus(cattle)
Product	prothrombin precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from J00041.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: J00041.1, BC105201.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145419, SAMN03145422 [ECO:0000348] ##Evidence-Data-END##

ATGGCGCGCG TCCGAGGCCC GCGGCTGCCT GGCTGCCTGG CCCTGGCTGC CCTGTTCAGC 
CTCGTGCACA GCCAGCATGT GTTCCTGGCC CATCAGCAAG CATCCTCGCT GCTCCAGAGG 
GCCCGCCGTG CCAACAAGGG CTTCCTGGAG GAGGTGCGGA AGGGCAACCT GGAGCGAGAG 
TGCCTGGAGG AGCCATGCAG CCGCGAGGAG GCCTTCGAGG CCCTGGAGTC TCTCAGTGCC 
ACGGATGCGT TCTGGGCCAA GTACACAGCT TGTGAGTCAG CGAGAAATCC TCGAGAAAAG 
CTCAATGAAT GTCTGGAAGG AAACTGCGCT GAAGGTGTGG GGATGAACTA CCGAGGGAAC 
GTGAGCGTCA CCCGGTCAGG CATCGAGTGC CAGCTGTGGA GAAGTCGCTA CCCACATAAG 
CCAGAAATCA ACTCTACCAC CCACCCGGGG GCTGACCTGC GGGAGAATTT TTGCCGCAAC 
CCGGATGGCA GCATTACTGG GCCCTGGTGC TACACCACAT CCCCGACTCT GCGGAGAGAA 
GAGTGCAGCG TCCCGGTGTG CGGCCAGGAC CGAGTCACAG TGGAGGTGAT CCCCCGGTCG 
GGAGGCTCCA CTACCAGTCA GTCGCCTCTA CTGGAAACAT GCGTCCCGGA CCGCGGCCGG 
GAGTACCGGG GGCGACTGGC GGTGACCACA CACGGGTCCC GCTGCCTTGC CTGGAGCAGC 
GAGCAGGCCA AGGCCCTGAG CAAGCACCAG GACTTCAACC CGGCCGTGCC CCTGGCGGAG 
AACTTCTGCC GCAACCCAGA CGGGGACGAG GAGGGCGCCT GGTGCTACGT GGCCGACCAG 
CCTGGCGACT TTGAGTATTG TGACCTGAAC TACTGCGAGG AGCCGGTGGA TGGAGACCTG 
GGAGACAGGC TGGGTGAGGA CCCGGACCCG GACGCGGCCA TCGAGGGACG CACGTCTGAG 
GACCATTTCC AGCCCTTCTT CAACGAGAAG ACCTTTGGCG CCGGGGAGGC CGACTGTGGC 
CTGCGACCCC TGTTCGAGAA GAAGCAGGTG CAGGACCAAA CGGAGAAGGA GCTTTTCGAG 
TCCTACATCG AGGGGCGCAT CGTGGAGGGT CAGGACGCGG AGGTTGGCCT CTCGCCCTGG 
CAGGTGATGC TCTTTCGTAA GAGTCCCCAG GAGCTGCTCT GTGGGGCCAG CCTCATCAGT 
GACCGCTGGG TCCTCACGGC TGCCCACTGT CTCCTGTACC CGCCTTGGGA CAAGAACTTC 
ACCGTGGATG ACCTGCTGGT GCGCATCGGC AAGCACTCCC GCACCAGGTA TGAGCGGAAG 
GTTGAAAAGA TCTCCATGCT GGACAAGATC TACATCCACC CCAGGTACAA CTGGAAGGAG 
AATCTGGACC GGGACATCGC CCTGCTGAAG CTCAAGAGGC CCATCGAGTT ATCCGACTAC 
ATCCACCCCG TGTGCCTGCC CGACAAGCAG ACAGCAGCCA AGCTGCTCCA CGCTGGGTTC 
AAAGGGCGGG TGACGGGCTG GGGCAACCGG AGGGAGACGT GGACCACCAG CGTGGCCGAG 
GTGCAGCCCA GCGTCCTCCA GGTGGTCAAC CTGCCTCTCG TGGAGCGGCC CGTGTGCAAG 
GCCTCCACCC GGATCCGCAT CACCGACAAC ATGTTCTGTG CCGGTTACAA GCCTGGTGAA 
GGCAAACGAG GGGACGCTTG TGAGGGCGAC AGCGGGGGAC CCTTCGTCAT GAAGAGCCCC 
TATAACAACC GCTGGTATCA AATGGGCATC GTCTCATGGG GTGAAGGCTG TGACAGGGAT 
GGAAAATATG GCTTCTACAC ACACGTCTTC CGCCTGAAGA AGTGGATACA GAAAGTCATT 
GATCGGTTAG GAAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_776302.1
CDS	6..1883
Translation	MARVRGPRLPGCLALAALFSLVHSQHVFLAHQQASSLLQRARRANKGFLEEVRKGNLERECLEEPCSREEAFEALESLSATDAFWAKYTACESARNPREKLNECLEGNCAEGVGMNYRGNVSVTRSGIECQLWRSRYPHKPEINSTTHPGADLRENFCRNPDGSITGPWCYTTSPTLRREECSVPVCGQDRVTVEVIPRSGGSTTSQSPLLETCVPDRGREYRGRLAVTTHGSRCLAWSSEQAKALSKHQDFNPAVPLAENFCRNPDGDEEGAWCYVADQPGDFEYCDLNYCEEPVDGDLGDRLGEDPDPDAAIEGRTSEDHFQPFFNEKTFGAGEADCGLRPLFEKKQVQDQTEKELFESYIEGRIVEGQDAEVGLSPWQVMLFRKSPQELLCGASLISDRWVLTAAHCLLYPPWDKNFTVDDLLVRIGKHSRTRYERKVEKISMLDKIYIHPRYNWKENLDRDIALLKLKRPIELSDYIHPVCLPDKQTAAKLLHAGFKGRVTGWGNRRETWTTSVAEVQPSVLQVVNLPLVERPVCKASTRIRITDNMFCAGYKPGEGKRGDACEGDSGGPFVMKSPYNNRWYQMGIVSWGEGCDRDGKYGFYTHVFRLKKWIQKVIDRLGS

Target ORF information:

RefSeq Version	NM_173877.1
Organism	Bos taurus(cattle)
Definition	Bos taurus coagulation factor II, thrombin (F2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_173877.1

ORF Insert Sequence:

ATGGCGCGCG TCCGAGGCCC GCGGCTGCCT GGCTGCCTGG CCCTGGCTGC CCTGTTCAGC 
CTCGTGCACA GCCAGCATGT GTTCCTGGCC CATCAGCAAG CATCCTCGCT GCTCCAGAGG 
GCCCGCCGTG CCAACAAGGG CTTCCTGGAG GAGGTGCGGA AGGGCAACCT GGAGCGAGAG 
TGCCTGGAGG AGCCATGCAG CCGCGAGGAG GCCTTCGAGG CCCTGGAGTC TCTCAGTGCC 
ACGGATGCGT TCTGGGCCAA GTACACAGCT TGTGAGTCAG CGAGAAATCC TCGAGAAAAG 
CTCAATGAAT GTCTGGAAGG AAACTGCGCT GAAGGTGTGG GGATGAACTA CCGAGGGAAC 
GTGAGCGTCA CCCGGTCAGG CATCGAGTGC CAGCTGTGGA GAAGTCGCTA CCCACATAAG 
CCAGAAATCA ACTCTACCAC CCACCCGGGG GCTGACCTGC GGGAGAATTT TTGCCGCAAC 
CCGGATGGCA GCATTACTGG GCCCTGGTGC TACACCACAT CCCCGACTCT GCGGAGAGAA 
GAGTGCAGCG TCCCGGTGTG CGGCCAGGAC CGAGTCACAG TGGAGGTGAT CCCCCGGTCG 
GGAGGCTCCA CTACCAGTCA GTCGCCTCTA CTGGAAACAT GCGTCCCGGA CCGCGGCCGG 
GAGTACCGGG GGCGACTGGC GGTGACCACA CACGGGTCCC GCTGCCTTGC CTGGAGCAGC 
GAGCAGGCCA AGGCCCTGAG CAAGCACCAG GACTTCAACC CGGCCGTGCC CCTGGCGGAG 
AACTTCTGCC GCAACCCAGA CGGGGACGAG GAGGGCGCCT GGTGCTACGT GGCCGACCAG 
CCTGGCGACT TTGAGTATTG TGACCTGAAC TACTGCGAGG AGCCGGTGGA TGGAGACCTG 
GGAGACAGGC TGGGTGAGGA CCCGGACCCG GACGCGGCCA TCGAGGGACG CACGTCTGAG 
GACCATTTCC AGCCCTTCTT CAACGAGAAG ACCTTTGGCG CCGGGGAGGC CGACTGTGGC 
CTGCGACCCC TGTTCGAGAA GAAGCAGGTG CAGGACCAAA CGGAGAAGGA GCTTTTCGAG 
TCCTACATCG AGGGGCGCAT CGTGGAGGGT CAGGACGCGG AGGTTGGCCT CTCGCCCTGG 
CAGGTGATGC TCTTTCGTAA GAGTCCCCAG GAGCTGCTCT GTGGGGCCAG CCTCATCAGT 
GACCGCTGGG TCCTCACGGC TGCCCACTGT CTCCTGTACC CGCCTTGGGA CAAGAACTTC 
ACCGTGGATG ACCTGCTGGT GCGCATCGGC AAGCACTCCC GCACCAGGTA TGAGCGGAAG 
GTTGAAAAGA TCTCCATGCT GGACAAGATC TACATCCACC CCAGGTACAA CTGGAAGGAG 
AATCTGGACC GGGACATCGC CCTGCTGAAG CTCAAGAGGC CCATCGAGTT ATCCGACTAC 
ATCCACCCCG TGTGCCTGCC CGACAAGCAG ACAGCAGCCA AGCTGCTCCA CGCTGGGTTC 
AAAGGGCGGG TGACGGGCTG GGGCAACCGG AGGGAGACGT GGACCACCAG CGTGGCCGAG 
GTGCAGCCCA GCGTCCTCCA GGTGGTCAAC CTGCCTCTCG TGGAGCGGCC CGTGTGCAAG 
GCCTCCACCC GGATCCGCAT CACCGACAAC ATGTTCTGTG CCGGTTACAA GCCTGGTGAA 
GGCAAACGAG GGGACGCTTG TGAGGGCGAC AGCGGGGGAC CCTTCGTCAT GAAGAGCCCC 
TATAACAACC GCTGGTATCA AATGGGCATC GTCTCATGGG GTGAAGGCTG TGACAGGGAT 
GGAAAATATG GCTTCTACAC ACACGTCTTC CGCCTGAAGA AGTGGATACA GAAAGTCATT 
GATCGGTTAG GAAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

F2 ( XM_005216401.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005216458.1 Bos taurus coagulation factor II, thrombin (F2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa26705
Clone ID Related Accession (Same CDS sequence)	XM_005216401.3
Accession Version	XM_005216401.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1896bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	prothrombin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104421.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005216401.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGCG TCCGAGGCCC GCGGCTGCCT GGCTGCCTGG CCCTGGCTGC CCTGTTCAGC 
CTCGTGCACA GCCAGCATGT GTTCCTGGCC CATCAGCAAG CATCCTCGCT GCTCCAGAGG 
GCCCGCCGTG CCAACAAGGG CTTCCTGGAG GAGGTGCGGA AGGGCAACCT GGAGCGAGAG 
TGCCTGGAGG AGCCATGCAG CCGCGAGGAG GCCTTCGAGG CCCTGGAGTC TCTCAGTGCC 
ACGGATGCGT TCTGGGCCAA GTACACAGCT TGTGAGTCAG CGAGAAATCC TCGAGAAAAG 
CTCAATGAAT GTCTGGAAGG AAACTGCGCT GAAGGTGTGG GGATGAACTA CCGAGGGAAC 
GTGAGCGTCA CCCGGTCAGG CATCGAGTGC CAGCTGTGGA GAAGTCGCTA CCCACATAAG 
CCAGAAATCA ACTCTACCAC CCACCCGGGG GCTGACCTGC GGGAGAATTT TTGCCGCAAC 
CCGGATGGCA GCATTACTGG GCCCTGGTGC TACACCACAT CCCCGACTCT GCGGAGAGAA 
GAGTGCAGCG TCCCCGTGTG CGGCCAGGAC CGAGTCACAG TGGAGGTGAT CCCCCGGTCA 
GGAGGCTCCA CTACCAGTCA GTCGCCTCTA CTGGAAACAT GCGTCCCGGA CCGCGGCCGG 
GAGTACCGGG GGCGGCTGGC GGTGACCACA AGCGGGTCCC GCTGCCTTGC CTGGAGCAGC 
GAGCAGGCCA AGGCCCTGAG CAAGGACCAG GACTTCAACC CGGCCGTGCC CCTGGCGGAG 
AACTTCTGCC GCAACCCAGA CGGGGACGAG GAGGGCGCCT GGTGCTACGT GGCCGACCAG 
CCTGGCGACT TTGAGTATTG TGACCTGAAC TACTGCGAGG AGCCGGTGGA TGGAGACCTG 
GGAGACAGGC TGGGTGAGGA CCCGGACCCG GACGCGGCCA TCGAGGGACG CACGTCTGAG 
GACCATTTCC AGCCCTTCTT CAACGAGAAG ACCTTTGGCG CCGGGGAGGC CGACTGTGGC 
CTGCGACCCC TGTTCGAGAA GAAGCAGGTG CAGGACCAAA CGGAGAAGGA GCTTTTCGAG 
TCCTACATCG AGGGGCGCAT CGTGGAGGGT CAGGACGCGG AGGTTGGCCT CTCGCCCTGG 
CAGGTGATGC TCTTTCGTAA GAGTCCCCAG GAGCTGCTCT GTGGGGCCAG CCTCATCAGT 
GACCGCTGGG TCCTCACGGC TGCCCACTGT CTCCTGTACC CGCCTTGGGA CAAGAACTTC 
ACCGTGGATG ACCTGCTGGT GCGCATCGGC AAGCACTCCC GCACCAGGTG CGGAGGGGCC 
CCCAGGTATG AGCGGAAGGT TGAAAAGATC TCCATGCTGG ACAAGATCTA CATCCACCCC 
AGGTACAACT GGAAGGAGAA TCTGGACCGG GACATCGCCC TGCTGAAGCT CAAGAGGCCC 
ATCGAGTTAT CCGACTACAT CCACCCCGTG TGCCTGCCCG ACAAGCAGAC AGCAGCCAAG 
CTGCTCCACG CTGGGTTCAA AGGGCGGGTG ACGGGCTGGG GCAACCGGAG GGAGACGTGG 
ACCACCAGCG TGGCCGAGGT GCAGCCCAGC GTCCTCCAGG TGGTCAACCT GCCTCTCGTG 
GAGCGGCCCG TGTGCAAGGC CTCCACCCGG ATCCGCATCA CCGACAACAT GTTCTGTGCC 
GGTTACAAGC CTGGTGAAGG CAAACGAGGG GACGCTTGTG AGGGCGACAG CGGGGGACCC 
TTCGTCATGA AGAGCCCCTA TAACAACCGC TGGTATCAAA TGGGCATCGT CTCATGGGGT 
GAAGGCTGTG ACAGGGATGG AAAATATGGC TTCTACACAC ACGTCTTCCG CCTGAAGAAG 
TGGATACAGA AAGTCATTGA TCGGTTAGGA AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005216458.1
CDS	215..2110
Translation	MARVRGPRLPGCLALAALFSLVHSQHVFLAHQQASSLLQRARRANKGFLEEVRKGNLERECLEEPCSREEAFEALESLSATDAFWAKYTACESARNPREKLNECLEGNCAEGVGMNYRGNVSVTRSGIECQLWRSRYPHKPEINSTTHPGADLRENFCRNPDGSITGPWCYTTSPTLRREECSVPVCGQDRVTVEVIPRSGGSTTSQSPLLETCVPDRGREYRGRLAVTTSGSRCLAWSSEQAKALSKDQDFNPAVPLAENFCRNPDGDEEGAWCYVADQPGDFEYCDLNYCEEPVDGDLGDRLGEDPDPDAAIEGRTSEDHFQPFFNEKTFGAGEADCGLRPLFEKKQVQDQTEKELFESYIEGRIVEGQDAEVGLSPWQVMLFRKSPQELLCGASLISDRWVLTAAHCLLYPPWDKNFTVDDLLVRIGKHSRTRCGGAPRYERKVEKISMLDKIYIHPRYNWKENLDRDIALLKLKRPIELSDYIHPVCLPDKQTAAKLLHAGFKGRVTGWGNRRETWTTSVAEVQPSVLQVVNLPLVERPVCKASTRIRITDNMFCAGYKPGEGKRGDACEGDSGGPFVMKSPYNNRWYQMGIVSWGEGCDRDGKYGFYTHVFRLKKWIQKVIDRLGS

Target ORF information:

RefSeq Version	XM_005216401.3
Organism	Bos taurus(cattle)
Definition	Bos taurus coagulation factor II, thrombin (F2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005216401.3

ORF Insert Sequence:

ATGGCGCGCG TCCGAGGCCC GCGGCTGCCT GGCTGCCTGG CCCTGGCTGC CCTGTTCAGC 
CTCGTGCACA GCCAGCATGT GTTCCTGGCC CATCAGCAAG CATCCTCGCT GCTCCAGAGG 
GCCCGCCGTG CCAACAAGGG CTTCCTGGAG GAGGTGCGGA AGGGCAACCT GGAGCGAGAG 
TGCCTGGAGG AGCCATGCAG CCGCGAGGAG GCCTTCGAGG CCCTGGAGTC TCTCAGTGCC 
ACGGATGCGT TCTGGGCCAA GTACACAGCT TGTGAGTCAG CGAGAAATCC TCGAGAAAAG 
CTCAATGAAT GTCTGGAAGG AAACTGCGCT GAAGGTGTGG GGATGAACTA CCGAGGGAAC 
GTGAGCGTCA CCCGGTCAGG CATCGAGTGC CAGCTGTGGA GAAGTCGCTA CCCACATAAG 
CCAGAAATCA ACTCTACCAC CCACCCGGGG GCTGACCTGC GGGAGAATTT TTGCCGCAAC 
CCGGATGGCA GCATTACTGG GCCCTGGTGC TACACCACAT CCCCGACTCT GCGGAGAGAA 
GAGTGCAGCG TCCCCGTGTG CGGCCAGGAC CGAGTCACAG TGGAGGTGAT CCCCCGGTCA 
GGAGGCTCCA CTACCAGTCA GTCGCCTCTA CTGGAAACAT GCGTCCCGGA CCGCGGCCGG 
GAGTACCGGG GGCGGCTGGC GGTGACCACA AGCGGGTCCC GCTGCCTTGC CTGGAGCAGC 
GAGCAGGCCA AGGCCCTGAG CAAGGACCAG GACTTCAACC CGGCCGTGCC CCTGGCGGAG 
AACTTCTGCC GCAACCCAGA CGGGGACGAG GAGGGCGCCT GGTGCTACGT GGCCGACCAG 
CCTGGCGACT TTGAGTATTG TGACCTGAAC TACTGCGAGG AGCCGGTGGA TGGAGACCTG 
GGAGACAGGC TGGGTGAGGA CCCGGACCCG GACGCGGCCA TCGAGGGACG CACGTCTGAG 
GACCATTTCC AGCCCTTCTT CAACGAGAAG ACCTTTGGCG CCGGGGAGGC CGACTGTGGC 
CTGCGACCCC TGTTCGAGAA GAAGCAGGTG CAGGACCAAA CGGAGAAGGA GCTTTTCGAG 
TCCTACATCG AGGGGCGCAT CGTGGAGGGT CAGGACGCGG AGGTTGGCCT CTCGCCCTGG 
CAGGTGATGC TCTTTCGTAA GAGTCCCCAG GAGCTGCTCT GTGGGGCCAG CCTCATCAGT 
GACCGCTGGG TCCTCACGGC TGCCCACTGT CTCCTGTACC CGCCTTGGGA CAAGAACTTC 
ACCGTGGATG ACCTGCTGGT GCGCATCGGC AAGCACTCCC GCACCAGGTG CGGAGGGGCC 
CCCAGGTATG AGCGGAAGGT TGAAAAGATC TCCATGCTGG ACAAGATCTA CATCCACCCC 
AGGTACAACT GGAAGGAGAA TCTGGACCGG GACATCGCCC TGCTGAAGCT CAAGAGGCCC 
ATCGAGTTAT CCGACTACAT CCACCCCGTG TGCCTGCCCG ACAAGCAGAC AGCAGCCAAG 
CTGCTCCACG CTGGGTTCAA AGGGCGGGTG ACGGGCTGGG GCAACCGGAG GGAGACGTGG 
ACCACCAGCG TGGCCGAGGT GCAGCCCAGC GTCCTCCAGG TGGTCAACCT GCCTCTCGTG 
GAGCGGCCCG TGTGCAAGGC CTCCACCCGG ATCCGCATCA CCGACAACAT GTTCTGTGCC 
GGTTACAAGC CTGGTGAAGG CAAACGAGGG GACGCTTGTG AGGGCGACAG CGGGGGACCC 
TTCGTCATGA AGAGCCCCTA TAACAACCGC TGGTATCAAA TGGGCATCGT CTCATGGGGT 
GAAGGCTGTG ACAGGGATGG AAAATATGGC TTCTACACAC ACGTCTTCCG CCTGAAGAAG 
TGGATACAGA AAGTCATTGA TCGGTTAGGA AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

F2 ( XM_005216401.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005216458.2 Bos taurus coagulation factor II, thrombin (F2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa222607
Clone ID Related Accession (Same CDS sequence)	XM_005216401.4
Accession Version	XM_005216401.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1896bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	prothrombin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005216401.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGCG TCCGAGGCCC GCGGCTGCCT GGCTGCCTGG CCCTGGCTGC CCTGTTCAGC 
CTCGTGCACA GCCAGCATGT GTTCCTGGCC CATCAGCAAG CATCCTCGCT GCTCCAGAGG 
GCCCGCCGTG CCAACAAGGG CTTCCTGGAG GAGGTGCGGA AGGGCAACCT GGAGCGAGAG 
TGCCTGGAGG AGCCATGCAG CCGCGAGGAG GCCTTCGAGG CCCTGGAGTC TCTCAGTGCC 
ACGGATGCGT TCTGGGCCAA GTACACAGCT TGTGAGTCAG CGAGAAATCC TCGAGAAAAG 
CTCAATGAAT GTCTGGAAGG AAACTGCGCT GAAGGTGTGG GGATGAACTA CCGAGGGAAC 
GTGAGCGTCA CCCGGTCAGG CATCGAGTGC CAGCTGTGGA GAAGTCGCTA CCCACATAAG 
CCAGAAATCA ACTCTACCAC CCACCCGGGG GCTGACCTGC GGGAGAATTT TTGCCGCAAC 
CCGGATGGCA GCATTACTGG GCCCTGGTGC TACACCACAT CCCCGACTCT GCGGAGAGAA 
GAGTGCAGCG TCCCGGTGTG CGGCCAGGAC CGAGTCACAG TGGAGGTGAT CCCCCGGTCG 
GGAGGCTCCA CTACCAGTCA GTCGCCTCTA CTGGAAACAT GCGTCCCGGA CCGCGGCCGG 
GAGTACCGGG GGCGGCTGGC GGTGACCACA CGCGGGTCCC GCTGCCTTGC CTGGAGCAGC 
GAGCAGGCCA AGGCCCTGAG CAAGGACCAG GACTTCAACC CGGCCGTGCC CCTGGCGGAG 
AACTTCTGCC GCAACCCAGA CGGGGACGAG GAGGGCGCCT GGTGCTACGT GGCCGACCAG 
CCTGGCGACT TTGAGTATTG TGACCTGAAC TACTGCGAGG AGCCGGTGGA TGGAGACCTG 
GGAGACAGGC TGGGTGAGGA CCCGGACCCG GACGCGGCCA TCGAGGGACG CACGTCTGAG 
GACCATTTCC AGCCCTTCTT CAACGAGAAG ACCTTTGGCG CCGGGGAGGC CGACTGTGGC 
CTGCGACCCC TGTTCGAGAA GAAGCAGGTG CAGGACCAAA CGGAGAAGGA GCTTTTCGAG 
TCCTACATCG AGGGGCGCAT CGTGGAGGGT CAGGACGCGG AGGTTGGCCT CTCGCCCTGG 
CAGGTGATGC TCTTTCGTAA GAGTCCCCAG GAGCTGCTCT GTGGGGCCAG CCTCATCAGT 
GACCGCTGGG TCCTCACGGC TGCCCACTGT CTCCTGTACC CGCCTTGGGA CAAGAACTTC 
ACCGTGGATG ACCTGCTGGT GCGCATCGGC AAGCACTCCC GCACCAGGTG CGGAGGGGCC 
CCCAGGTATG AGCGGAAGGT TGAAAAGATC TCCATGCTGG ACAAGATCTA CATCCACCCC 
AGGTACAACT GGAAGGAGAA TCTGGACCGG GACATCGCCC TGCTGAAGCT CAAGAGGCCC 
ATCGAGTTAT CCGACTACAT CCACCCCGTG TGCCTGCCCG ACAAGCAGAC AGCAGCCAAG 
CTGCTCCACG CTGGGTTCAA AGGGCGGGTG ACGGGCTGGG GCAACCGGAG GGAGACGTGG 
ACCACCAGCG TGGCCGAGGT GCAGCCCAGC GTCCTCCAGG TGGTCAACCT GCCTCTCGTG 
GAGCGGCCCG TGTGCAAGGC CTCCACCCGG ATCCGCATCA CCGACAACAT GTTCTGTGCC 
GGTTACAAGC CTGGTGAAGG CAAACGAGGG GACGCTTGTG AGGGCGACAG CGGGGGACCC 
TTCGTCATGA AGAGCCCCTA TAACAACCGC TGGTATCAAA TGGGCATCGT CTCATGGGGT 
GAAGGCTGTG ACAGGGATGG AAAATATGGC TTCTACACAC ACGTCTTCCG CCTGAAGAAG 
TGGATACAGA AAGTCATTGA TCGGTTAGGA AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005216458.2
CDS	227..2122
Translation	MARVRGPRLPGCLALAALFSLVHSQHVFLAHQQASSLLQRARRANKGFLEEVRKGNLERECLEEPCSREEAFEALESLSATDAFWAKYTACESARNPREKLNECLEGNCAEGVGMNYRGNVSVTRSGIECQLWRSRYPHKPEINSTTHPGADLRENFCRNPDGSITGPWCYTTSPTLRREECSVPVCGQDRVTVEVIPRSGGSTTSQSPLLETCVPDRGREYRGRLAVTTRGSRCLAWSSEQAKALSKDQDFNPAVPLAENFCRNPDGDEEGAWCYVADQPGDFEYCDLNYCEEPVDGDLGDRLGEDPDPDAAIEGRTSEDHFQPFFNEKTFGAGEADCGLRPLFEKKQVQDQTEKELFESYIEGRIVEGQDAEVGLSPWQVMLFRKSPQELLCGASLISDRWVLTAAHCLLYPPWDKNFTVDDLLVRIGKHSRTRCGGAPRYERKVEKISMLDKIYIHPRYNWKENLDRDIALLKLKRPIELSDYIHPVCLPDKQTAAKLLHAGFKGRVTGWGNRRETWTTSVAEVQPSVLQVVNLPLVERPVCKASTRIRITDNMFCAGYKPGEGKRGDACEGDSGGPFVMKSPYNNRWYQMGIVSWGEGCDRDGKYGFYTHVFRLKKWIQKVIDRLGS

Target ORF information:

RefSeq Version	XM_005216401.4
Organism	Bos taurus(cattle)
Definition	Bos taurus coagulation factor II, thrombin (F2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005216401.4

ORF Insert Sequence:

ATGGCGCGCG TCCGAGGCCC GCGGCTGCCT GGCTGCCTGG CCCTGGCTGC CCTGTTCAGC 
CTCGTGCACA GCCAGCATGT GTTCCTGGCC CATCAGCAAG CATCCTCGCT GCTCCAGAGG 
GCCCGCCGTG CCAACAAGGG CTTCCTGGAG GAGGTGCGGA AGGGCAACCT GGAGCGAGAG 
TGCCTGGAGG AGCCATGCAG CCGCGAGGAG GCCTTCGAGG CCCTGGAGTC TCTCAGTGCC 
ACGGATGCGT TCTGGGCCAA GTACACAGCT TGTGAGTCAG CGAGAAATCC TCGAGAAAAG 
CTCAATGAAT GTCTGGAAGG AAACTGCGCT GAAGGTGTGG GGATGAACTA CCGAGGGAAC 
GTGAGCGTCA CCCGGTCAGG CATCGAGTGC CAGCTGTGGA GAAGTCGCTA CCCACATAAG 
CCAGAAATCA ACTCTACCAC CCACCCGGGG GCTGACCTGC GGGAGAATTT TTGCCGCAAC 
CCGGATGGCA GCATTACTGG GCCCTGGTGC TACACCACAT CCCCGACTCT GCGGAGAGAA 
GAGTGCAGCG TCCCGGTGTG CGGCCAGGAC CGAGTCACAG TGGAGGTGAT CCCCCGGTCG 
GGAGGCTCCA CTACCAGTCA GTCGCCTCTA CTGGAAACAT GCGTCCCGGA CCGCGGCCGG 
GAGTACCGGG GGCGGCTGGC GGTGACCACA CGCGGGTCCC GCTGCCTTGC CTGGAGCAGC 
GAGCAGGCCA AGGCCCTGAG CAAGGACCAG GACTTCAACC CGGCCGTGCC CCTGGCGGAG 
AACTTCTGCC GCAACCCAGA CGGGGACGAG GAGGGCGCCT GGTGCTACGT GGCCGACCAG 
CCTGGCGACT TTGAGTATTG TGACCTGAAC TACTGCGAGG AGCCGGTGGA TGGAGACCTG 
GGAGACAGGC TGGGTGAGGA CCCGGACCCG GACGCGGCCA TCGAGGGACG CACGTCTGAG 
GACCATTTCC AGCCCTTCTT CAACGAGAAG ACCTTTGGCG CCGGGGAGGC CGACTGTGGC 
CTGCGACCCC TGTTCGAGAA GAAGCAGGTG CAGGACCAAA CGGAGAAGGA GCTTTTCGAG 
TCCTACATCG AGGGGCGCAT CGTGGAGGGT CAGGACGCGG AGGTTGGCCT CTCGCCCTGG 
CAGGTGATGC TCTTTCGTAA GAGTCCCCAG GAGCTGCTCT GTGGGGCCAG CCTCATCAGT 
GACCGCTGGG TCCTCACGGC TGCCCACTGT CTCCTGTACC CGCCTTGGGA CAAGAACTTC 
ACCGTGGATG ACCTGCTGGT GCGCATCGGC AAGCACTCCC GCACCAGGTG CGGAGGGGCC 
CCCAGGTATG AGCGGAAGGT TGAAAAGATC TCCATGCTGG ACAAGATCTA CATCCACCCC 
AGGTACAACT GGAAGGAGAA TCTGGACCGG GACATCGCCC TGCTGAAGCT CAAGAGGCCC 
ATCGAGTTAT CCGACTACAT CCACCCCGTG TGCCTGCCCG ACAAGCAGAC AGCAGCCAAG 
CTGCTCCACG CTGGGTTCAA AGGGCGGGTG ACGGGCTGGG GCAACCGGAG GGAGACGTGG 
ACCACCAGCG TGGCCGAGGT GCAGCCCAGC GTCCTCCAGG TGGTCAACCT GCCTCTCGTG 
GAGCGGCCCG TGTGCAAGGC CTCCACCCGG ATCCGCATCA CCGACAACAT GTTCTGTGCC 
GGTTACAAGC CTGGTGAAGG CAAACGAGGG GACGCTTGTG AGGGCGACAG CGGGGGACCC 
TTCGTCATGA AGAGCCCCTA TAACAACCGC TGGTATCAAA TGGGCATCGT CTCATGGGGT 
GAAGGCTGTG ACAGGGATGG AAAATATGGC TTCTACACAC ACGTCTTCCG CCTGAAGAAG 
TGGATACAGA AAGTCATTGA TCGGTTAGGA AGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

F2 ( XM_015474857.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330343.1 Bos taurus coagulation factor II, thrombin (F2), transcript variant X2, mRNA.

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CloneID	OBa92794
Clone ID Related Accession (Same CDS sequence)	XM_015474857.1
Accession Version	XM_015474857.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1878bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	prothrombin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104421.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGCG TCCGAGGCCC GCGGCTGCCT GGCTGCCTGG CCCTGGCTGC CCTGTTCAGC 
CTCGTGCACA GCCAGCATGT GTTCCTGGCC CATCAGCAAG CATCCTCGCT GCTCCAGAGG 
GCCCGCCGTG CCAACAAGGG CTTCCTGGAG GAGGTGCGGA AGGGCAACCT GGAGCGAGAG 
TGCCTGGAGG AGCCATGCAG CCGCGAGGAG GCCTTCGAGG CCCTGGAGTC TCTCAGTGCC 
ACGGATGCGT TCTGGGCCAA GTACACAGCT TGTGAGTCAG CGAGAAATCC TCGAGAAAAG 
CTCAATGAAT GTCTGGAAGG AAACTGCGCT GAAGGTGTGG GGATGAACTA CCGAGGGAAC 
GTGAGCGTCA CCCGGTCAGG CATCGAGTGC CAGCTGTGGA GAAGTCGCTA CCCACATAAG 
CCAGAAATCA ACTCTACCAC CCACCCGGGG GCTGACCTGC GGGAGAATTT TTGCCGCAAC 
CCGGATGGCA GCATTACTGG GCCCTGGTGC TACACCACAT CCCCGACTCT GCGGAGAGAA 
GAGTGCAGCG TCCCCGTGTG CGGCCAGGAC CGAGTCACAG TGGAGGTGAT CCCCCGGTCA 
GGAGGCTCCA CTACCAGTCA GTCGCCTCTA CTGGAAACAT GCGTCCCGGA CCGCGGCCGG 
GAGTACCGGG GGCGGCTGGC GGTGACCACA AGCGGGTCCC GCTGCCTTGC CTGGAGCAGC 
GAGCAGGCCA AGGCCCTGAG CAAGGACCAG GACTTCAACC CGGCCGTGCC CCTGGCGGAG 
AACTTCTGCC GCAACCCAGA CGGGGACGAG GAGGGCGCCT GGTGCTACGT GGCCGACCAG 
CCTGGCGACT TTGAGTATTG TGACCTGAAC TACTGCGAGG AGCCGGTGGA TGGAGACCTG 
GGAGACAGGC TGGGTGAGGA CCCGGACCCG GACGCGGCCA TCGAGGGACG CACGTCTGAG 
GACCATTTCC AGCCCTTCTT CAACGAGAAG ACCTTTGGCG CCGGGGAGGC CGACTGTGGC 
CTGCGACCCC TGTTCGAGAA GAAGCAGGTG CAGGACCAAA CGGAGAAGGA GCTTTTCGAG 
TCCTACATCG AGGGGCGCAT CGTGGAGGGT CAGGACGCGG AGGTTGGCCT CTCGCCCTGG 
CAGGTGATGC TCTTTCGTAA GAGTCCCCAG GAGCTGCTCT GTGGGGCCAG CCTCATCAGT 
GACCGCTGGG TCCTCACGGC TGCCCACTGT CTCCTGTACC CGCCTTGGGA CAAGAACTTC 
ACCGTGGATG ACCTGCTGGT GCGCATCGGC AAGCACTCCC GCACCAGGTA TGAGCGGAAG 
GTTGAAAAGA TCTCCATGCT GGACAAGATC TACATCCACC CCAGGTACAA CTGGAAGGAG 
AATCTGGACC GGGACATCGC CCTGCTGAAG CTCAAGAGGC CCATCGAGTT ATCCGACTAC 
ATCCACCCCG TGTGCCTGCC CGACAAGCAG ACAGCAGCCA AGCTGCTCCA CGCTGGGTTC 
AAAGGGCGGG TGACGGGCTG GGGCAACCGG AGGGAGACGT GGACCACCAG CGTGGCCGAG 
GTGCAGCCCA GCGTCCTCCA GGTGGTCAAC CTGCCTCTCG TGGAGCGGCC CGTGTGCAAG 
GCCTCCACCC GGATCCGCAT CACCGACAAC ATGTTCTGTG CCGGTTACAA GCCTGGTGAA 
GGCAAACGAG GGGACGCTTG TGAGGGCGAC AGCGGGGGAC CCTTCGTCAT GAAGAGCCCC 
TATAACAACC GCTGGTATCA AATGGGCATC GTCTCATGGG GTGAAGGCTG TGACAGGGAT 
GGAAAATATG GCTTCTACAC ACACGTCTTC CGCCTGAAGA AGTGGATACA GAAAGTCATT 
GATCGGTTAG GAAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330343.1
CDS	213..2090
Translation	MARVRGPRLPGCLALAALFSLVHSQHVFLAHQQASSLLQRARRANKGFLEEVRKGNLERECLEEPCSREEAFEALESLSATDAFWAKYTACESARNPREKLNECLEGNCAEGVGMNYRGNVSVTRSGIECQLWRSRYPHKPEINSTTHPGADLRENFCRNPDGSITGPWCYTTSPTLRREECSVPVCGQDRVTVEVIPRSGGSTTSQSPLLETCVPDRGREYRGRLAVTTSGSRCLAWSSEQAKALSKDQDFNPAVPLAENFCRNPDGDEEGAWCYVADQPGDFEYCDLNYCEEPVDGDLGDRLGEDPDPDAAIEGRTSEDHFQPFFNEKTFGAGEADCGLRPLFEKKQVQDQTEKELFESYIEGRIVEGQDAEVGLSPWQVMLFRKSPQELLCGASLISDRWVLTAAHCLLYPPWDKNFTVDDLLVRIGKHSRTRYERKVEKISMLDKIYIHPRYNWKENLDRDIALLKLKRPIELSDYIHPVCLPDKQTAAKLLHAGFKGRVTGWGNRRETWTTSVAEVQPSVLQVVNLPLVERPVCKASTRIRITDNMFCAGYKPGEGKRGDACEGDSGGPFVMKSPYNNRWYQMGIVSWGEGCDRDGKYGFYTHVFRLKKWIQKVIDRLGS

Target ORF information:

RefSeq Version	XM_015474857.1
Organism	Bos taurus(cattle)
Definition	Bos taurus coagulation factor II, thrombin (F2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015474857.1

ORF Insert Sequence:

ATGGCGCGCG TCCGAGGCCC GCGGCTGCCT GGCTGCCTGG CCCTGGCTGC CCTGTTCAGC 
CTCGTGCACA GCCAGCATGT GTTCCTGGCC CATCAGCAAG CATCCTCGCT GCTCCAGAGG 
GCCCGCCGTG CCAACAAGGG CTTCCTGGAG GAGGTGCGGA AGGGCAACCT GGAGCGAGAG 
TGCCTGGAGG AGCCATGCAG CCGCGAGGAG GCCTTCGAGG CCCTGGAGTC TCTCAGTGCC 
ACGGATGCGT TCTGGGCCAA GTACACAGCT TGTGAGTCAG CGAGAAATCC TCGAGAAAAG 
CTCAATGAAT GTCTGGAAGG AAACTGCGCT GAAGGTGTGG GGATGAACTA CCGAGGGAAC 
GTGAGCGTCA CCCGGTCAGG CATCGAGTGC CAGCTGTGGA GAAGTCGCTA CCCACATAAG 
CCAGAAATCA ACTCTACCAC CCACCCGGGG GCTGACCTGC GGGAGAATTT TTGCCGCAAC 
CCGGATGGCA GCATTACTGG GCCCTGGTGC TACACCACAT CCCCGACTCT GCGGAGAGAA 
GAGTGCAGCG TCCCCGTGTG CGGCCAGGAC CGAGTCACAG TGGAGGTGAT CCCCCGGTCA 
GGAGGCTCCA CTACCAGTCA GTCGCCTCTA CTGGAAACAT GCGTCCCGGA CCGCGGCCGG 
GAGTACCGGG GGCGGCTGGC GGTGACCACA AGCGGGTCCC GCTGCCTTGC CTGGAGCAGC 
GAGCAGGCCA AGGCCCTGAG CAAGGACCAG GACTTCAACC CGGCCGTGCC CCTGGCGGAG 
AACTTCTGCC GCAACCCAGA CGGGGACGAG GAGGGCGCCT GGTGCTACGT GGCCGACCAG 
CCTGGCGACT TTGAGTATTG TGACCTGAAC TACTGCGAGG AGCCGGTGGA TGGAGACCTG 
GGAGACAGGC TGGGTGAGGA CCCGGACCCG GACGCGGCCA TCGAGGGACG CACGTCTGAG 
GACCATTTCC AGCCCTTCTT CAACGAGAAG ACCTTTGGCG CCGGGGAGGC CGACTGTGGC 
CTGCGACCCC TGTTCGAGAA GAAGCAGGTG CAGGACCAAA CGGAGAAGGA GCTTTTCGAG 
TCCTACATCG AGGGGCGCAT CGTGGAGGGT CAGGACGCGG AGGTTGGCCT CTCGCCCTGG 
CAGGTGATGC TCTTTCGTAA GAGTCCCCAG GAGCTGCTCT GTGGGGCCAG CCTCATCAGT 
GACCGCTGGG TCCTCACGGC TGCCCACTGT CTCCTGTACC CGCCTTGGGA CAAGAACTTC 
ACCGTGGATG ACCTGCTGGT GCGCATCGGC AAGCACTCCC GCACCAGGTA TGAGCGGAAG 
GTTGAAAAGA TCTCCATGCT GGACAAGATC TACATCCACC CCAGGTACAA CTGGAAGGAG 
AATCTGGACC GGGACATCGC CCTGCTGAAG CTCAAGAGGC CCATCGAGTT ATCCGACTAC 
ATCCACCCCG TGTGCCTGCC CGACAAGCAG ACAGCAGCCA AGCTGCTCCA CGCTGGGTTC 
AAAGGGCGGG TGACGGGCTG GGGCAACCGG AGGGAGACGT GGACCACCAG CGTGGCCGAG 
GTGCAGCCCA GCGTCCTCCA GGTGGTCAAC CTGCCTCTCG TGGAGCGGCC CGTGTGCAAG 
GCCTCCACCC GGATCCGCAT CACCGACAAC ATGTTCTGTG CCGGTTACAA GCCTGGTGAA 
GGCAAACGAG GGGACGCTTG TGAGGGCGAC AGCGGGGGAC CCTTCGTCAT GAAGAGCCCC 
TATAACAACC GCTGGTATCA AATGGGCATC GTCTCATGGG GTGAAGGCTG TGACAGGGAT 
GGAAAATATG GCTTCTACAC ACACGTCTTC CGCCTGAAGA AGTGGATACA GAAAGTCATT 
GATCGGTTAG GAAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Kinetic regulation of the binding of prothrombin to phospholipid membranes.
Molecular and cellular biochemistry382(1-2)193-201(2013 Oct)
Smith E,Vekaria R,Brown KA,Longstaff C

Anticoagulant activity of a unique sulfated pyranosic (1->3)-?-L-arabinan through direct interaction with thrombin.
The Journal of biological chemistry288(1)223-33(2013 Jan)
Fern?ndez PV,Quintana I,Cerezo AS,Caramelo JJ,Pol-Fachin L,Verli H,Estevez JM,Ciancia M

Exogenous thrombin delivery promotes collateral capillary arterialization and tissue reperfusion in the murine spinotrapezius muscle ischemia model.
Microcirculation (New York, N.Y. : 1994)19(2)143-54(2012 Feb)
Bruce AC,Peirce SM

Thrombin induces inducible nitric oxide synthase expression via the MAPK, MSK1, and NF-?B signaling pathways in alveolar macrophages.
European journal of pharmacology672(1-3)180-7(2011 Dec)
Lin CC,Shih CH,Yang YL,Bien MY,Lin CH,Yu MC,Sureshbabu M,Chen BC

High glucose enhances thrombin responses via protease-activated receptor-4 in human vascular smooth muscle cells.
Arteriosclerosis, thrombosis, and vascular biology31(3)624-33(2011 Mar)
Dangwal S,Rauch BH,Gensch T,Dai L,Bretschneider E,Vogelaar CF,Schr?r K,Rosenkranz AC

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F2 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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