Adcy8 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Adcy8
Entrez Gene ID	29241
Full Name	adenylate cyclase 8
Synonyms	Ac8
General protein information	Preferred Names adenylate cyclase 8 Names adenylate cyclase type 8 ATP pyrophosphate-lyase 8 adenylate cyclase 8 (brain) adenylate cyclase type VIII adenylyl cyclase 8 ca(2+)/calmodulin-activated adenylyl cyclase type VIII adenylyl cyclase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q33
Summary	may play a role in calcium/calmodulin regulated intracellular signaling; may mediate long term potentiation [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006241703.3	XP_006241765.1	adenylate cyclase type 8 isoform X1
XM_006241704.3	XP_006241766.1	adenylate cyclase type 8 isoform X2
NM_017142.1	NP_058838.1	adenylate cyclase type 8

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	ADCY8	XP_418437.2
Xenopus tropicalis (tropical clawed frog)	adcy8	NP_001096207.1
Bos taurus (cattle)	ADCY8	NP_001179770.1
Drosophila melanogaster (fruit fly)	Ac78C	NP_001262137.1
Homo sapiens (human)	ADCY8	NP_001106.1
	ADCY8	XP_001084919.1
Rattus norvegicus (Norway rat)	Adcy8	NP_058838.1
Pan troglodytes (chimpanzee)	ADCY8	XP_519960.2
Canis lupus familiaris (dog)	ADCY8	XP_539166.2
Danio rerio (zebrafish)	adcy8	NP_001137224.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002262	XP_307919.5
Mus musculus (house mouse)	Adcy8	NP_033753.2

Gene Ontology
Bibliography

Related articles in PubMed

Calcium activated adenylyl cyclase AC8 but not AC1 is required for prolonged behavioral anxiety.
Bernabucci M, Zhuo M
Molecular brain9(1)60(2016 05)

Multilevel control of glucose homeostasis by adenylyl cyclase 8.
Raoux M, Vacher P, Papin J, Picard A, Kostrzewa E, Devin A, Gaitan J, Limon I, Kas MJ, Magnan C, Lang J
Diabetologia58(4)749-57(2015 Apr)

Calcium influx activates adenylyl cyclase 8 for sustained insulin secretion in rat pancreatic beta cells.
Dou H, Wang C, Wu X, Yao L, Zhang X, Teng S, Xu H, Liu B, Wu Q, Zhang Q, Hu M, Wang Y, Wang L, Wu Y, Shang S, Kang X, Zheng L, Zhang J, Raoux M, Lang J, Li Q, Su J, Yu X, Chen L, Zhou Z
Diabetologia58(2)324-33(2015 Feb)

A key phosphorylation site in AC8 mediates regulation of Ca(2+)-dependent cAMP dynamics by an AC8-AKAP79-PKA signalling complex.
Willoughby D, Halls ML, Everett KL, Ciruela A, Skroblin P, Klussmann E, Cooper DM
Journal of cell science125(Pt 23)5850-9(2012 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Despite previous hints pointing to a major contribution of ACs, the presented data show that there is no apparent causality between AC activity and the occurrence of the epileptic phenotype.
Title: Adenylyl cyclases: expression in the developing rat thalamus and their role in absence epilepsy.

AC8 expression, besides being induced by the proinflammatory cytokine IL1beta, is also dependent on down-regulation of the Notch pathway occurring in an inflammatory context.
Title: The Notch pathway attenuates interleukin 1? (IL1?)-mediated induction of adenylyl cyclase 8 (AC8) expression during vascular smooth muscle cell (VSMC) trans-differentiation.

Mutation of the two critical cysteines results in exclusion of AKAP79 from lipid rafts and alterations in its membrane diffusion behavior and is accompanied by a loss of the ability of AKAP79 to regulate AC8.
Title: Palmitoylation targets AKAP79 protein to lipid rafts and promotes its regulation of calcium-sensitive adenylyl cyclase type 8.

cAMP-mediated pathways are modelled by glucose, and downregulation of the calcium-sensitive ADCY8 plays a central role herein, including signalling via the GLP1R.
Title: Adenylyl cyclase 8 is central to glucagon-like peptide 1 signalling and effects of chronically elevated glucose in rat and human pancreatic beta cells.

An IQ-like motif imparts calmodulin binding and autoinhibitory functions to adenylyl cyclcase 8.
Title: Separate elements within a single IQ-like motif in adenylyl cyclase type 8 impart ca2+/calmodulin binding and autoinhibition.

The following Adcy8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Adcy8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa03002	NM_017142.1 Latest version!	Rattus norvegicus adenylate cyclase 8 (Adcy8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$916.30 $1309.00
ORa30406	XM_006241703.3 Latest version!	Rattus norvegicus adenylate cyclase 8 (Adcy8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
ORa30407	XM_006241704.3 Latest version!	Rattus norvegicus adenylate cyclase 8 (Adcy8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Adcy8 ( NM_017142.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_058838.1 Rattus norvegicus adenylate cyclase 8 (Adcy8), mRNA.

Price & Availability↑

CloneID	ORa03002
Clone ID Related Accession (Same CDS sequence)	NM_017142.1
Accession Version	NM_017142.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3747bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	adenylate cyclase type 8
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from L26986.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L26986.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGAACTCT CGGATGTGCA CTGCCTTAGC GGCAGCGAGG AACTCTATAC CATTCACCCG 
ACGCCCCCGG CCGCGGACGG CGGGAGCGGC TCTCGGCCGC AGCGGCTGCT GTGGCAGACG 
GCGGTGCGGC ACATCACCGA GCAGCGCTTC ATCCACGGCC ACCGAGGCGG CGGCGGCGGG 
GGCTCCCGCA AAGCCTCGAA CCCTGCGGGC AGCGGACCAA ACCACCACGC GCCGCAGCTG 
TCTAGCGACT CGGTGCTGCC TCTCTATTCT CTGGGCTCCG GAGAGCGAGC GCACAACACC 
GGTGGCACCA AAGTCTTTCC GGAACGCAGC GGGAGCGGCA GTGCCAGTGG CAGCGGGGGT 
GGGGGCGACT TGGGCTTCCT GCACCTTGAC TGTGCCCCAA GTAACTCGGA TTTCTTCCTC 
AATGGGGGAT ACAGCTACCG TGGGGTCATT TTCCCAACCC TACGCAACTC CTTCAAGTCT 
CGGGATCTGG AGCGCCTCTA TCAACGCTAT TTCCTGGGCC AGAGGCGCAA ATCGGAGGTA 
GTGATGAACG TGCTGGATGT ACTAACCAAA CTCACCCTTC TAGTCCTTCA CTTGAGCCTA 
GCCTCGGCTC CCATGGACCC TCTCAAGGGC ATCCTGCTAG GCTTTTTCAC TGGCATCGAG 
GTGGTGATCT GCGCCCTCGT GGTGGTCAGG AAGGACACCA CCTCCCACAC TTACCTGCAA 
TACAGCGGCG TGGTCACTTG GGTGGCTATG ACCACCCAGA TTCTGGCAGC AGGCCTGGGC 
TATGGGCTTC TGGGCGACGG CATAGGCTAC GTGCTTTTTA CACTCTTCGC CACCTACAGC 
ATGCTTCCGC TGCCTCTCAC CTGGGCCATC TTGGCCGGCC TGGGCACATC CTTGCTGCAA 
GTCACACTTC AAGTGCTCAT ACCCAGACTA GCGGTCTTTT CCATCAACCA GGTCCTGGCC 
CAGGTGGTGC TCTTCATGTG CATGAATACA GCAGGCATCT TCATCAGTTA CCTTTCAGAC 
CGCGCCCAGC GGCAGGCCTT CCTGGAGACC CGGAGGTGTG TGGAGGCCAG GCTCCGCCTG 
GAGACAGAGA ACCAAAGACA GGAGCGGCTT GTGCTCTCTG TGCTCCCCAG GTTTGTCGTC 
CTAGAAATGA TCAATGACAT GACCAATGTG GAGGACGAGC ACCTGCAGCA TCAGTTCCAC 
CGCATCTACA TCCATCGCTA CGAGAACGTC AGTATTCTTT TTGCAGATGT CAAAGGATTT 
ACCAACCTCT CTACGACCTT GTCTGCTCAG GAGCTTGTCA GGATGCTCAA CGAGCTCTTT 
GCCAGATTTG ATCGGCTGGC CCATGAGCAT CACTGTCTTC GCATTAAAAT CCTGGGGGAC 
TGCTACTACT GTGTGTCAGG ACTGCCTGAG CCCCGCCAGG ACCATGCTCA TTGCTGTGTT 
GAAATGGGCC TCAGCATGAT CAAAACTATC AGGTTTGTGA GGTCCAGAAC GAAGCATGAT 
GTTGACATGC GAATTGGAAT CCATTCAGGC TCTGTGCTGT GTGGTGTGTT GGGCCTGAGA 
AAATGGCAGT TTGATGTCTG GTCTTGGGAT GTGGACATCG CAAACAAACT TGAATCTGGA 
GGAATCCCTG GGAGAATTCA CATTTCCAAA GCCACACTGG ATTGCCTCAG TGGTGACTAT 
AATGTGGAAG AGGGCCACGG TAAGGAGAGG AATGAATTCT TGAGGAAGCA TAATATAGAG 
ACCTATTTGA TTAAGCAGCC CGAGGAGAGT TTGCTATCCT TGCCTGAAGA TATAGTTAAG 
GAGTCGGTGA GCTGCTCGGA CAGGAGAAAC AGTGGGGCAA CGTTCACAGA AGGATCCTGG 
AGCCCAGAAC TGCCATTCGA CAACATCGTG GGCAAACAGA ATACTCTGGC TGCCCTAACA 
AGAAATTCAA TAAATCTGCT TCCAAACCAT CTCGCACAAG CTTTGCATGT CCAGTCTGGG 
CCTGAGGAAA TTAACAAGAG AATAGAGCAT ACCATCGACT TGCGGAGTGG CGATAAGTTG 
AGAAGAGAGC ATATCAAGCC ATTCTCACTG ATGTTTAAAG ACTCCAGCCT GGAGCACAAG 
TATTCTCAAA TGCGGGATGA AGTATTCAAG TCAAACTTGG TCTGTGCATT TATCGTTCTT 
CTGTTTATCA CTGCGATTCA AAGTTTGCTT CCTTCTTCGA GGCTGATGCC TATGACCATC 
CAGTTCTCCA TCCTGATCAT GCTGCACTCC GCCCTGGTCC TCATCACCAC GGCAGAAGAC 
TATAAGTGTC TGCCTCTCAT TCTCCGCAAA ACCTGTTGTT GGATTAACGA GACCTATTTG 
GCCCGCAACG TCATCATCTT TGCTTCCATC TTGATTAATT TCCTGGGAGC CGTCATAAAT 
ATTCTGTGGT GTGATTTTGA CAAGTCGATA CCCTTGAAGA ACCTGACTTT CAATTCCTCA 
GCTGTGTTTA CAGATATCTG CTCCTACCCA GAGTACTTTG TCTTCACTGG GGTGTTGGCC 
ATGGTGACGT GTGCAGTATT TCTCCGGCTT AACTCTGTCC TGAAGCTGGC TGTGCTGCTC 
ATTATGATCG CCATCTACGC CCTGCTGACA GAGACCATCT ATGCAGGTCT CTTTCTGAGT 
TATGACAACC TGAACCACAG TGGAGAAGAT TTTCTGGGGA CCAAGGAGGC ATCACTGCTA 
CTGATGGCCA TGTTCCTTCT TGCTGTATTC TACCATGGAC AGCAGCTGGA GTACACAGCC 
CGCCTAGATT TCCTGTGGCG AGTACAGGCC AAAGAGGAGA TCAACGAGAT GAAGGACTTG 
AGGGAACACA ATGAGAACAT GCTTCGCAAT ATCTTACCCG GCCACGTGGC CCGCCACTTC 
CTGGAGAAAG ACAGAGACAA TGAGGAGCTG TATTCTCAAT CCTATGATGC CGTTGGGGTA 
ATGTTTGCCT CCATTCCTGG ATTTGCAGAC TTTTACTCTC AGACAGAAAT GAACAACCAG 
GGAGTGGAAT GTCTGCGCTT GCTGAATGAG ATCATTGCTG ACTTTGATGA GTTACTTGGA 
GAGGACCGCT TTCAGGACAT AGAGAAGATT AAGACCATTG GTAGTACATA CATGGCTGTC 
TCAGGACTGT CACCAGAGAA ACAGCAATGT GAAGATAAAT GGGGACATTT GTGTGCCCTG 
GCTGACTTCT CTCTTGCCCT GACTGAAAGC ATACAAGAGA TCAACAAGCA TTCGTTCAAC 
AATTTTGAAC TCCGTATTGG CATCAGCCAT GGCTCAGTGG TAGCAGGTGT AATTGGCGCT 
AAGAAACCAC AGTATGACAT TTGGGGTAAA ACTGTGAACT TGGCAAGCCG AATGGACAGC 
ACAGGAGTGA GTGGCCGGAT CCAAGTTCCT GAGGAGACCT ATCTCATCCT GAAGGATCAG 
GGCTTTGCCT TCGACTACCG GGGAGAGATA TATGTGAAGG GCATCAGCGA ACAAGAAGGG 
AAAATCAAAA CATATTTTCT CCTGGGACGA GTCCAACCCA ACCCATTCAT CTTACCCCCA 
AGAAGACTTC CTGGGCAGTA CTCTCTGGCT GCAGTTGTCC TTGGGCTTGT CCAGTCTCTC 
AACAGGCAAA GGCAGAAGCA ACTACTCAAT GAGAACAGCA ACTCTGGCAT CATCAAGAGC 
CATTACAACC GGCGGACTTT GCTAACGCCA AGTGGGCCAG AGCCTGGAGC ACAGGCTGAA 
GGCACTGACA AATCCGATTT GCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058838.1
CDS	777..4523
Translation	MELSDVHCLSGSEELYTIHPTPPAADGGSGSRPQRLLWQTAVRHITEQRFIHGHRGGGGGGSRKASNPAGSGPNHHAPQLSSDSVLPLYSLGSGERAHNTGGTKVFPERSGSGSASGSGGGGDLGFLHLDCAPSNSDFFLNGGYSYRGVIFPTLRNSFKSRDLERLYQRYFLGQRRKSEVVMNVLDVLTKLTLLVLHLSLASAPMDPLKGILLGFFTGIEVVICALVVVRKDTTSHTYLQYSGVVTWVAMTTQILAAGLGYGLLGDGIGYVLFTLFATYSMLPLPLTWAILAGLGTSLLQVTLQVLIPRLAVFSINQVLAQVVLFMCMNTAGIFISYLSDRAQRQAFLETRRCVEARLRLETENQRQERLVLSVLPRFVVLEMINDMTNVEDEHLQHQFHRIYIHRYENVSILFADVKGFTNLSTTLSAQELVRMLNELFARFDRLAHEHHCLRIKILGDCYYCVSGLPEPRQDHAHCCVEMGLSMIKTIRFVRSRTKHDVDMRIGIHSGSVLCGVLGLRKWQFDVWSWDVDIANKLESGGIPGRIHISKATLDCLSGDYNVEEGHGKERNEFLRKHNIETYLIKQPEESLLSLPEDIVKESVSCSDRRNSGATFTEGSWSPELPFDNIVGKQNTLAALTRNSINLLPNHLAQALHVQSGPEEINKRIEHTIDLRSGDKLRREHIKPFSLMFKDSSLEHKYSQMRDEVFKSNLVCAFIVLLFITAIQSLLPSSRLMPMTIQFSILIMLHSALVLITTAEDYKCLPLILRKTCCWINETYLARNVIIFASILINFLGAVINILWCDFDKSIPLKNLTFNSSAVFTDICSYPEYFVFTGVLAMVTCAVFLRLNSVLKLAVLLIMIAIYALLTETIYAGLFLSYDNLNHSGEDFLGTKEASLLLMAMFLLAVFYHGQQLEYTARLDFLWRVQAKEEINEMKDLREHNENMLRNILPGHVARHFLEKDRDNEELYSQSYDAVGVMFASIPGFADFYSQTEMNNQGVECLRLLNEIIADFDELLGEDRFQDIEKIKTIGSTYMAVSGLSPEKQQCEDKWGHLCALADFSLALTESIQEINKHSFNNFELRIGISHGSVVAGVIGAKKPQYDIWGKTVNLASRMDSTGVSGRIQVPEETYLILKDQGFAFDYRGEIYVKGISEQEGKIKTYFLLGRVQPNPFILPPRRLPGQYSLAAVVLGLVQSLNRQRQKQLLNENSNSGIIKSHYNRRTLLTPSGPEPGAQAEGTDKSDLP

Target ORF information:

RefSeq Version	NM_017142.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus adenylate cyclase 8 (Adcy8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017142.1

ORF Insert Sequence:

ATGGAACTCT CGGATGTGCA CTGCCTTAGC GGCAGCGAGG AACTCTATAC CATTCACCCG 
ACGCCCCCGG CCGCGGACGG CGGGAGCGGC TCTCGGCCGC AGCGGCTGCT GTGGCAGACG 
GCGGTGCGGC ACATCACCGA GCAGCGCTTC ATCCACGGCC ACCGAGGCGG CGGCGGCGGG 
GGCTCCCGCA AAGCCTCGAA CCCTGCGGGC AGCGGACCAA ACCACCACGC GCCGCAGCTG 
TCTAGCGACT CGGTGCTGCC TCTCTATTCT CTGGGCTCCG GAGAGCGAGC GCACAACACC 
GGTGGCACCA AAGTCTTTCC GGAACGCAGC GGGAGCGGCA GTGCCAGTGG CAGCGGGGGT 
GGGGGCGACT TGGGCTTCCT GCACCTTGAC TGTGCCCCAA GTAACTCGGA TTTCTTCCTC 
AATGGGGGAT ACAGCTACCG TGGGGTCATT TTCCCAACCC TACGCAACTC CTTCAAGTCT 
CGGGATCTGG AGCGCCTCTA TCAACGCTAT TTCCTGGGCC AGAGGCGCAA ATCGGAGGTA 
GTGATGAACG TGCTGGATGT ACTAACCAAA CTCACCCTTC TAGTCCTTCA CTTGAGCCTA 
GCCTCGGCTC CCATGGACCC TCTCAAGGGC ATCCTGCTAG GCTTTTTCAC TGGCATCGAG 
GTGGTGATCT GCGCCCTCGT GGTGGTCAGG AAGGACACCA CCTCCCACAC TTACCTGCAA 
TACAGCGGCG TGGTCACTTG GGTGGCTATG ACCACCCAGA TTCTGGCAGC AGGCCTGGGC 
TATGGGCTTC TGGGCGACGG CATAGGCTAC GTGCTTTTTA CACTCTTCGC CACCTACAGC 
ATGCTTCCGC TGCCTCTCAC CTGGGCCATC TTGGCCGGCC TGGGCACATC CTTGCTGCAA 
GTCACACTTC AAGTGCTCAT ACCCAGACTA GCGGTCTTTT CCATCAACCA GGTCCTGGCC 
CAGGTGGTGC TCTTCATGTG CATGAATACA GCAGGCATCT TCATCAGTTA CCTTTCAGAC 
CGCGCCCAGC GGCAGGCCTT CCTGGAGACC CGGAGGTGTG TGGAGGCCAG GCTCCGCCTG 
GAGACAGAGA ACCAAAGACA GGAGCGGCTT GTGCTCTCTG TGCTCCCCAG GTTTGTCGTC 
CTAGAAATGA TCAATGACAT GACCAATGTG GAGGACGAGC ACCTGCAGCA TCAGTTCCAC 
CGCATCTACA TCCATCGCTA CGAGAACGTC AGTATTCTTT TTGCAGATGT CAAAGGATTT 
ACCAACCTCT CTACGACCTT GTCTGCTCAG GAGCTTGTCA GGATGCTCAA CGAGCTCTTT 
GCCAGATTTG ATCGGCTGGC CCATGAGCAT CACTGTCTTC GCATTAAAAT CCTGGGGGAC 
TGCTACTACT GTGTGTCAGG ACTGCCTGAG CCCCGCCAGG ACCATGCTCA TTGCTGTGTT 
GAAATGGGCC TCAGCATGAT CAAAACTATC AGGTTTGTGA GGTCCAGAAC GAAGCATGAT 
GTTGACATGC GAATTGGAAT CCATTCAGGC TCTGTGCTGT GTGGTGTGTT GGGCCTGAGA 
AAATGGCAGT TTGATGTCTG GTCTTGGGAT GTGGACATCG CAAACAAACT TGAATCTGGA 
GGAATCCCTG GGAGAATTCA CATTTCCAAA GCCACACTGG ATTGCCTCAG TGGTGACTAT 
AATGTGGAAG AGGGCCACGG TAAGGAGAGG AATGAATTCT TGAGGAAGCA TAATATAGAG 
ACCTATTTGA TTAAGCAGCC CGAGGAGAGT TTGCTATCCT TGCCTGAAGA TATAGTTAAG 
GAGTCGGTGA GCTGCTCGGA CAGGAGAAAC AGTGGGGCAA CGTTCACAGA AGGATCCTGG 
AGCCCAGAAC TGCCATTCGA CAACATCGTG GGCAAACAGA ATACTCTGGC TGCCCTAACA 
AGAAATTCAA TAAATCTGCT TCCAAACCAT CTCGCACAAG CTTTGCATGT CCAGTCTGGG 
CCTGAGGAAA TTAACAAGAG AATAGAGCAT ACCATCGACT TGCGGAGTGG CGATAAGTTG 
AGAAGAGAGC ATATCAAGCC ATTCTCACTG ATGTTTAAAG ACTCCAGCCT GGAGCACAAG 
TATTCTCAAA TGCGGGATGA AGTATTCAAG TCAAACTTGG TCTGTGCATT TATCGTTCTT 
CTGTTTATCA CTGCGATTCA AAGTTTGCTT CCTTCTTCGA GGCTGATGCC TATGACCATC 
CAGTTCTCCA TCCTGATCAT GCTGCACTCC GCCCTGGTCC TCATCACCAC GGCAGAAGAC 
TATAAGTGTC TGCCTCTCAT TCTCCGCAAA ACCTGTTGTT GGATTAACGA GACCTATTTG 
GCCCGCAACG TCATCATCTT TGCTTCCATC TTGATTAATT TCCTGGGAGC CGTCATAAAT 
ATTCTGTGGT GTGATTTTGA CAAGTCGATA CCCTTGAAGA ACCTGACTTT CAATTCCTCA 
GCTGTGTTTA CAGATATCTG CTCCTACCCA GAGTACTTTG TCTTCACTGG GGTGTTGGCC 
ATGGTGACGT GTGCAGTATT TCTCCGGCTT AACTCTGTCC TGAAGCTGGC TGTGCTGCTC 
ATTATGATCG CCATCTACGC CCTGCTGACA GAGACCATCT ATGCAGGTCT CTTTCTGAGT 
TATGACAACC TGAACCACAG TGGAGAAGAT TTTCTGGGGA CCAAGGAGGC ATCACTGCTA 
CTGATGGCCA TGTTCCTTCT TGCTGTATTC TACCATGGAC AGCAGCTGGA GTACACAGCC 
CGCCTAGATT TCCTGTGGCG AGTACAGGCC AAAGAGGAGA TCAACGAGAT GAAGGACTTG 
AGGGAACACA ATGAGAACAT GCTTCGCAAT ATCTTACCCG GCCACGTGGC CCGCCACTTC 
CTGGAGAAAG ACAGAGACAA TGAGGAGCTG TATTCTCAAT CCTATGATGC CGTTGGGGTA 
ATGTTTGCCT CCATTCCTGG ATTTGCAGAC TTTTACTCTC AGACAGAAAT GAACAACCAG 
GGAGTGGAAT GTCTGCGCTT GCTGAATGAG ATCATTGCTG ACTTTGATGA GTTACTTGGA 
GAGGACCGCT TTCAGGACAT AGAGAAGATT AAGACCATTG GTAGTACATA CATGGCTGTC 
TCAGGACTGT CACCAGAGAA ACAGCAATGT GAAGATAAAT GGGGACATTT GTGTGCCCTG 
GCTGACTTCT CTCTTGCCCT GACTGAAAGC ATACAAGAGA TCAACAAGCA TTCGTTCAAC 
AATTTTGAAC TCCGTATTGG CATCAGCCAT GGCTCAGTGG TAGCAGGTGT AATTGGCGCT 
AAGAAACCAC AGTATGACAT TTGGGGTAAA ACTGTGAACT TGGCAAGCCG AATGGACAGC 
ACAGGAGTGA GTGGCCGGAT CCAAGTTCCT GAGGAGACCT ATCTCATCCT GAAGGATCAG 
GGCTTTGCCT TCGACTACCG GGGAGAGATA TATGTGAAGG GCATCAGCGA ACAAGAAGGG 
AAAATCAAAA CATATTTTCT CCTGGGACGA GTCCAACCCA ACCCATTCAT CTTACCCCCA 
AGAAGACTTC CTGGGCAGTA CTCTCTGGCT GCAGTTGTCC TTGGGCTTGT CCAGTCTCTC 
AACAGGCAAA GGCAGAAGCA ACTACTCAAT GAGAACAGCA ACTCTGGCAT CATCAAGAGC 
CATTACAACC GGCGGACTTT GCTAACGCCA AGTGGGCCAG AGCCTGGAGC ACAGGCTGAA 
GGCACTGACA AATCCGATTT GCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Adcy8 ( XM_006241703.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006241765.1 Rattus norvegicus adenylate cyclase 8 (Adcy8), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa30406
Clone ID Related Accession (Same CDS sequence)	XM_006241703.3
Accession Version	XM_006241703.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3657bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	adenylate cyclase type 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006241703.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACTCT CGGATGTGCA CTGCCTTAGC GGCAGCGAGG AACTCTATAC CATTCACCCG 
ACGCCCCCGG CCGGCGACGG CGGGAGCGGC TCTCGGCCGC AGCGGCTGCT GTGGCAGACG 
GCGGTGCGGC ACATCACCGA GCAGCGCTTC ATCCACGGCC ACCGAGGCGG CGGCGGCGGG 
GGCTCCCGCA AAGCCTCGAA CCCTGCGGGC AGCGGACCAA ACCACCACGC GCCGCAGCTG 
TCTAGCGACT CGGTGCTGCC TCTCTATTCT CTGGGCTCCG GAGAGCGAGC GCACAACACC 
GGTGGCACCA AAGTCTTTCC GGAACGCAGC GGGAGCGGCA GTGCCAGTGG CAGCGGGGGT 
GGGGGCGACT TGGGCTTCCT GCACCTTGAC TGTGCCCCAA GTAACTCGGA TTTCTTCCTC 
AATGGGGGAT ACAGCTACCG TGGGGTCATT TTCCCAACCC TACGCAACTC CTTCAAGTCT 
CGGGATCTGG AGCGCCTCTA TCAACGCTAT TTCCTGGGCC AGAGGCGCAA ATCGGAGGTA 
GTGATGAACG TGCTGGATGT ACTAACCAAA CTCACCCTTC TAGTCCTTCA CTTGAGCCTA 
GCCTCGGCTC CCATGGACCC TCTCAAGGGC ATCCTGCTAG GCTTTTTCAC TGGCATCGAG 
GTGGTGATCT GCGCCCTCGT GGTGGTCAGG AAGGACACCA CCTCCCACAC TTACCTGCAA 
TACAGCGGCG TGGTCACTTG GGTGGCTATG ACCACCCAGA TTCTGGCAGC AGGCCTGGGC 
TATGGGCTTC TGGGCGACGG CATAGGCTAC GTGCTTTTTA CACTCTTCGC CACCTACAGC 
ATGCTTCCGC TGCCTCTCAC CTGGGCCATC TTGGCCGGCC TGGGCACATC CTTGCTGCAA 
GTCACACTTC AAGTGCTCAT ACCCAGACTA GCGGTCTTTT CCATCAACCA GGTTCTGGCC 
CAGGTGGTGC TCTTCATGTG CATGAATACA GCAGGCATCT TCATCAGTTA CCTTTCAGAC 
CGCGCCCAGC GGCAGGCCTT CCTGGAGACC CGGAGGTGTG TGGAGGCCAG GCTCCGCCTG 
GAGACAGAGA ACCAAAGACA GGAGCGGCTT GTGCTCTCTG TGCTCCCCAG GTTTGTCGTC 
CTAGAAATGA TCAATGACAT GACCAATGTG GAGGACGAGC ACCTGCAGCA TCAGTTCCAC 
CGCATCTACA TCCATCGCTA CGAGAACGTC AGTATTCTTT TTGCAGATGT CAAAGGATTT 
ACCAACCTCT CTACGACCTT GTCTGCTCAG GAGCTTGTCA GGATGCTCAA CGAGCTCTTT 
GCCAGATTTG ATCGGCTGGC CCATGAGCAT CACTGTCTTC GCATTAAAAT CCTGGGGGAC 
TGCTACTACT GTGTGTCAGG ACTGCCTGAG CCCCGCCAGG ACCATGCTCA TTGCTGTGTT 
GAAATGGGCC TCAGCATGAT CAAAACTATC AGGTTTGTGA GGTCCAGAAC GAAGCATGAT 
GTTGACATGC GAATTGGAAT CCATTCAGGC TCTGTGCTGT GTGGTGTGTT GGGCCTGAGA 
AAATGGCAGT TTGATGTCTG GTCTTGGGAT GTGGACATCG CAAACAAACT TGAATCTGGA 
GGAATCCCTG GGAGAATTCA CATTTCCAAA GCCACACTGG ATTGCCTCAG TGGTGACTAT 
AATGTGGAAG AGGGCCACGG TAAGGAGAGG AATGAATTCT TGAGGAAGCA TAATATAGAG 
ACCTATTTGA TTAAGCAGCC CGAGGAGAGT TTGCTATCCT TGCCTGAAGA TATAGTTAAG 
GAGTCGGTGA GCTGCTCGGA CAGGAGAAAC AGTGGGGCAA CGTTCACAGA AGGATCCTGG 
AGCCCAGAAC TGCCATTCGA CAACATCGTG GGCAAACAGA ATACTCTGGC TGCCCTAACA 
AGAAATTCAA TAAATCTGCT TCCAAACCAT CTCGCACAAG CTTTGCATGT CCAGTCTGGG 
CCTGAGGAAA TTAACAAGAG AATAGAGCAT ACCATCGACT TGCGGAGTGG CGATAAGTTG 
AGAAGAGAGC ATATCAAGCC ATTCTCACTG ATGTTTAAAG ACTCCAGCCT GGAGCACAAG 
TATTCTCAAA TGCGGGATGA AGTATTCAAG TCAAACTTGG TCTGTGCATT TATCGTTCTT 
CTGTTTATCA CTGCGATTCA AAGTTTGCTT CCTTCTTCGA GGCTGATGCC TATGACCATC 
CAGTTCTCCA TCCTGATCAT GCTGCACTCC GCCCTGGTCC TCATCACCAC GGCAGAAGAC 
TATAAGTGTC TGCCTCTCAT TCTCCGCAAA ACCTGTTGTT GGATTAACGA GACCTATTTG 
GCCCGCAACG TCATCATCTT TGCTTCCATC TTGATTAATT TCCTGGGAGC CGTCATAAAT 
ATTTACTTTG TCTTCACTGG GGTGTTGGCC ATGGTGACGT GTGCAGTATT TCTCCGGCTT 
AACTCTGTCC TGAAGCTGGC TGTGCTGCTC ATTATGATCG CCATCTACGC CCTGCTGACA 
GAGACCATCT ATGCAGGTCT CTTTCTGAGT TATGACAACC TGAACCACAG TGGAGAAGAT 
TTTCTGGGGA CCAAGGAGGC ATCACTGCTA CTGATGGCCA TGTTCCTTCT TGCTGTATTC 
TACCATGGAC AGCAGCTGGA GTACACAGCC CGCCTAGATT TCCTGTGGCG AGTACAGGCC 
AAAGAGGAGA TCAACGAGAT GAAGGACTTG AGGGAACACA ATGAGAACAT GCTTCGCAAT 
ATCTTACCCG GCCACGTGGC CCGCCACTTC CTGGAGAAAG ACAGAGACAA TGAGGAGCTG 
TATTCTCAAT CCTATGATGC CGTTGGGGTA ATGTTTGCCT CCATTCCTGG ATTTGCAGAC 
TTTTACTCTC AGACAGAAAT GAACAACCAG GGAGTGGAAT GTCTGCGCTT GCTGAATGAG 
ATCATTGCTG ACTTTGATGA GTTACTTGGA GAGGACCGCT TTCAGGACAT AGAGAAGATT 
AAGACCATTG GTAGTACATA CATGGCTGTC TCAGGACTGT CACCAGAGAA ACAGCAATGT 
GAAGATAAAT GGGGACATTT GTGTGCCCTG GCTGACTTCT CTCTTGCCCT GACTGAAAGC 
ATACAAGAGA TCAACAAGCA TTCGTTCAAC AATTTTGAAC TCCGTATTGG CATCAGCCAT 
GGCTCAGTGG TAGCAGGTGT AATTGGCGCT AAGAAACCAC AGTATGACAT TTGGGGTAAA 
ACTGTGAACT TGGCAAGCCG AATGGACAGC ACAGGAGTGA GTGGCCGGAT CCAAGTTCCT 
GAGGAGACCT ATCTCATCCT GAAGGATCAG GGCTTTGCCT TCGACTACCG GGGAGAGATA 
TATGTGAAGG GCATCAGCGA ACAAGAAGGG AAAATCAAAA CATATTTTCT CCTGGGACGA 
GTCCAACCCA ACCCATTCAT CTTACCCCCA AGAAGACTTC CTGGGCAGTA CTCTCTGGCT 
GCAGTTGTCC TTGGCCTTGT CCAGTCTCTC AACAGGCAAA GGCAGAAGCA ACTACTCAAT 
GAGAACAGCA ACTCTGGCAT CATCAAGAGC CATTACAACC GGCGGACTTT GCTAACGCCA 
AGTGGGCCAG AGCCTGGAGC ACAGGCTGAA GGCACTGACA AATCCGATTT GCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006241765.1
CDS	1345..5001
Translation	MELSDVHCLSGSEELYTIHPTPPAGDGGSGSRPQRLLWQTAVRHITEQRFIHGHRGGGGGGSRKASNPAGSGPNHHAPQLSSDSVLPLYSLGSGERAHNTGGTKVFPERSGSGSASGSGGGGDLGFLHLDCAPSNSDFFLNGGYSYRGVIFPTLRNSFKSRDLERLYQRYFLGQRRKSEVVMNVLDVLTKLTLLVLHLSLASAPMDPLKGILLGFFTGIEVVICALVVVRKDTTSHTYLQYSGVVTWVAMTTQILAAGLGYGLLGDGIGYVLFTLFATYSMLPLPLTWAILAGLGTSLLQVTLQVLIPRLAVFSINQVLAQVVLFMCMNTAGIFISYLSDRAQRQAFLETRRCVEARLRLETENQRQERLVLSVLPRFVVLEMINDMTNVEDEHLQHQFHRIYIHRYENVSILFADVKGFTNLSTTLSAQELVRMLNELFARFDRLAHEHHCLRIKILGDCYYCVSGLPEPRQDHAHCCVEMGLSMIKTIRFVRSRTKHDVDMRIGIHSGSVLCGVLGLRKWQFDVWSWDVDIANKLESGGIPGRIHISKATLDCLSGDYNVEEGHGKERNEFLRKHNIETYLIKQPEESLLSLPEDIVKESVSCSDRRNSGATFTEGSWSPELPFDNIVGKQNTLAALTRNSINLLPNHLAQALHVQSGPEEINKRIEHTIDLRSGDKLRREHIKPFSLMFKDSSLEHKYSQMRDEVFKSNLVCAFIVLLFITAIQSLLPSSRLMPMTIQFSILIMLHSALVLITTAEDYKCLPLILRKTCCWINETYLARNVIIFASILINFLGAVINIYFVFTGVLAMVTCAVFLRLNSVLKLAVLLIMIAIYALLTETIYAGLFLSYDNLNHSGEDFLGTKEASLLLMAMFLLAVFYHGQQLEYTARLDFLWRVQAKEEINEMKDLREHNENMLRNILPGHVARHFLEKDRDNEELYSQSYDAVGVMFASIPGFADFYSQTEMNNQGVECLRLLNEIIADFDELLGEDRFQDIEKIKTIGSTYMAVSGLSPEKQQCEDKWGHLCALADFSLALTESIQEINKHSFNNFELRIGISHGSVVAGVIGAKKPQYDIWGKTVNLASRMDSTGVSGRIQVPEETYLILKDQGFAFDYRGEIYVKGISEQEGKIKTYFLLGRVQPNPFILPPRRLPGQYSLAAVVLGLVQSLNRQRQKQLLNENSNSGIIKSHYNRRTLLTPSGPEPGAQAEGTDKSDLP

Target ORF information:

RefSeq Version	XM_006241703.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus adenylate cyclase 8 (Adcy8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006241703.3

ORF Insert Sequence:

ATGGAACTCT CGGATGTGCA CTGCCTTAGC GGCAGCGAGG AACTCTATAC CATTCACCCG 
ACGCCCCCGG CCGGCGACGG CGGGAGCGGC TCTCGGCCGC AGCGGCTGCT GTGGCAGACG 
GCGGTGCGGC ACATCACCGA GCAGCGCTTC ATCCACGGCC ACCGAGGCGG CGGCGGCGGG 
GGCTCCCGCA AAGCCTCGAA CCCTGCGGGC AGCGGACCAA ACCACCACGC GCCGCAGCTG 
TCTAGCGACT CGGTGCTGCC TCTCTATTCT CTGGGCTCCG GAGAGCGAGC GCACAACACC 
GGTGGCACCA AAGTCTTTCC GGAACGCAGC GGGAGCGGCA GTGCCAGTGG CAGCGGGGGT 
GGGGGCGACT TGGGCTTCCT GCACCTTGAC TGTGCCCCAA GTAACTCGGA TTTCTTCCTC 
AATGGGGGAT ACAGCTACCG TGGGGTCATT TTCCCAACCC TACGCAACTC CTTCAAGTCT 
CGGGATCTGG AGCGCCTCTA TCAACGCTAT TTCCTGGGCC AGAGGCGCAA ATCGGAGGTA 
GTGATGAACG TGCTGGATGT ACTAACCAAA CTCACCCTTC TAGTCCTTCA CTTGAGCCTA 
GCCTCGGCTC CCATGGACCC TCTCAAGGGC ATCCTGCTAG GCTTTTTCAC TGGCATCGAG 
GTGGTGATCT GCGCCCTCGT GGTGGTCAGG AAGGACACCA CCTCCCACAC TTACCTGCAA 
TACAGCGGCG TGGTCACTTG GGTGGCTATG ACCACCCAGA TTCTGGCAGC AGGCCTGGGC 
TATGGGCTTC TGGGCGACGG CATAGGCTAC GTGCTTTTTA CACTCTTCGC CACCTACAGC 
ATGCTTCCGC TGCCTCTCAC CTGGGCCATC TTGGCCGGCC TGGGCACATC CTTGCTGCAA 
GTCACACTTC AAGTGCTCAT ACCCAGACTA GCGGTCTTTT CCATCAACCA GGTTCTGGCC 
CAGGTGGTGC TCTTCATGTG CATGAATACA GCAGGCATCT TCATCAGTTA CCTTTCAGAC 
CGCGCCCAGC GGCAGGCCTT CCTGGAGACC CGGAGGTGTG TGGAGGCCAG GCTCCGCCTG 
GAGACAGAGA ACCAAAGACA GGAGCGGCTT GTGCTCTCTG TGCTCCCCAG GTTTGTCGTC 
CTAGAAATGA TCAATGACAT GACCAATGTG GAGGACGAGC ACCTGCAGCA TCAGTTCCAC 
CGCATCTACA TCCATCGCTA CGAGAACGTC AGTATTCTTT TTGCAGATGT CAAAGGATTT 
ACCAACCTCT CTACGACCTT GTCTGCTCAG GAGCTTGTCA GGATGCTCAA CGAGCTCTTT 
GCCAGATTTG ATCGGCTGGC CCATGAGCAT CACTGTCTTC GCATTAAAAT CCTGGGGGAC 
TGCTACTACT GTGTGTCAGG ACTGCCTGAG CCCCGCCAGG ACCATGCTCA TTGCTGTGTT 
GAAATGGGCC TCAGCATGAT CAAAACTATC AGGTTTGTGA GGTCCAGAAC GAAGCATGAT 
GTTGACATGC GAATTGGAAT CCATTCAGGC TCTGTGCTGT GTGGTGTGTT GGGCCTGAGA 
AAATGGCAGT TTGATGTCTG GTCTTGGGAT GTGGACATCG CAAACAAACT TGAATCTGGA 
GGAATCCCTG GGAGAATTCA CATTTCCAAA GCCACACTGG ATTGCCTCAG TGGTGACTAT 
AATGTGGAAG AGGGCCACGG TAAGGAGAGG AATGAATTCT TGAGGAAGCA TAATATAGAG 
ACCTATTTGA TTAAGCAGCC CGAGGAGAGT TTGCTATCCT TGCCTGAAGA TATAGTTAAG 
GAGTCGGTGA GCTGCTCGGA CAGGAGAAAC AGTGGGGCAA CGTTCACAGA AGGATCCTGG 
AGCCCAGAAC TGCCATTCGA CAACATCGTG GGCAAACAGA ATACTCTGGC TGCCCTAACA 
AGAAATTCAA TAAATCTGCT TCCAAACCAT CTCGCACAAG CTTTGCATGT CCAGTCTGGG 
CCTGAGGAAA TTAACAAGAG AATAGAGCAT ACCATCGACT TGCGGAGTGG CGATAAGTTG 
AGAAGAGAGC ATATCAAGCC ATTCTCACTG ATGTTTAAAG ACTCCAGCCT GGAGCACAAG 
TATTCTCAAA TGCGGGATGA AGTATTCAAG TCAAACTTGG TCTGTGCATT TATCGTTCTT 
CTGTTTATCA CTGCGATTCA AAGTTTGCTT CCTTCTTCGA GGCTGATGCC TATGACCATC 
CAGTTCTCCA TCCTGATCAT GCTGCACTCC GCCCTGGTCC TCATCACCAC GGCAGAAGAC 
TATAAGTGTC TGCCTCTCAT TCTCCGCAAA ACCTGTTGTT GGATTAACGA GACCTATTTG 
GCCCGCAACG TCATCATCTT TGCTTCCATC TTGATTAATT TCCTGGGAGC CGTCATAAAT 
ATTTACTTTG TCTTCACTGG GGTGTTGGCC ATGGTGACGT GTGCAGTATT TCTCCGGCTT 
AACTCTGTCC TGAAGCTGGC TGTGCTGCTC ATTATGATCG CCATCTACGC CCTGCTGACA 
GAGACCATCT ATGCAGGTCT CTTTCTGAGT TATGACAACC TGAACCACAG TGGAGAAGAT 
TTTCTGGGGA CCAAGGAGGC ATCACTGCTA CTGATGGCCA TGTTCCTTCT TGCTGTATTC 
TACCATGGAC AGCAGCTGGA GTACACAGCC CGCCTAGATT TCCTGTGGCG AGTACAGGCC 
AAAGAGGAGA TCAACGAGAT GAAGGACTTG AGGGAACACA ATGAGAACAT GCTTCGCAAT 
ATCTTACCCG GCCACGTGGC CCGCCACTTC CTGGAGAAAG ACAGAGACAA TGAGGAGCTG 
TATTCTCAAT CCTATGATGC CGTTGGGGTA ATGTTTGCCT CCATTCCTGG ATTTGCAGAC 
TTTTACTCTC AGACAGAAAT GAACAACCAG GGAGTGGAAT GTCTGCGCTT GCTGAATGAG 
ATCATTGCTG ACTTTGATGA GTTACTTGGA GAGGACCGCT TTCAGGACAT AGAGAAGATT 
AAGACCATTG GTAGTACATA CATGGCTGTC TCAGGACTGT CACCAGAGAA ACAGCAATGT 
GAAGATAAAT GGGGACATTT GTGTGCCCTG GCTGACTTCT CTCTTGCCCT GACTGAAAGC 
ATACAAGAGA TCAACAAGCA TTCGTTCAAC AATTTTGAAC TCCGTATTGG CATCAGCCAT 
GGCTCAGTGG TAGCAGGTGT AATTGGCGCT AAGAAACCAC AGTATGACAT TTGGGGTAAA 
ACTGTGAACT TGGCAAGCCG AATGGACAGC ACAGGAGTGA GTGGCCGGAT CCAAGTTCCT 
GAGGAGACCT ATCTCATCCT GAAGGATCAG GGCTTTGCCT TCGACTACCG GGGAGAGATA 
TATGTGAAGG GCATCAGCGA ACAAGAAGGG AAAATCAAAA CATATTTTCT CCTGGGACGA 
GTCCAACCCA ACCCATTCAT CTTACCCCCA AGAAGACTTC CTGGGCAGTA CTCTCTGGCT 
GCAGTTGTCC TTGGCCTTGT CCAGTCTCTC AACAGGCAAA GGCAGAAGCA ACTACTCAAT 
GAGAACAGCA ACTCTGGCAT CATCAAGAGC CATTACAACC GGCGGACTTT GCTAACGCCA 
AGTGGGCCAG AGCCTGGAGC ACAGGCTGAA GGCACTGACA AATCCGATTT GCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Adcy8 ( XM_006241704.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006241766.1 Rattus norvegicus adenylate cyclase 8 (Adcy8), transcript variant X2, mRNA.

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CloneID	ORa30407
Clone ID Related Accession (Same CDS sequence)	XM_006241704.3
Accession Version	XM_006241704.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	adenylate cyclase type 8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006241704.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACTCT CGGATGTGCA CTGCCTTAGC GGCAGCGAGG AACTCTATAC CATTCACCCG 
ACGCCCCCGG CCGGCGACGG CGGGAGCGGC TCTCGGCCGC AGCGGCTGCT GTGGCAGACG 
GCGGTGCGGC ACATCACCGA GCAGCGCTTC ATCCACGGCC ACCGAGGCGG CGGCGGCGGG 
GGCTCCCGCA AAGCCTCGAA CCCTGCGGGC AGCGGACCAA ACCACCACGC GCCGCAGCTG 
TCTAGCGACT CGGTGCTGCC TCTCTATTCT CTGGGCTCCG GAGAGCGAGC GCACAACACC 
GGTGGCACCA AAGTCTTTCC GGAACGCAGC GGGAGCGGCA GTGCCAGTGG CAGCGGGGGT 
GGGGGCGACT TGGGCTTCCT GCACCTTGAC TGTGCCCCAA GTAACTCGGA TTTCTTCCTC 
AATGGGGGAT ACAGCTACCG TGGGGTCATT TTCCCAACCC TACGCAACTC CTTCAAGTCT 
CGGGATCTGG AGCGCCTCTA TCAACGCTAT TTCCTGGGCC AGAGGCGCAA ATCGGAGGTA 
GTGATGAACG TGCTGGATGT ACTAACCAAA CTCACCCTTC TAGTCCTTCA CTTGAGCCTA 
GCCTCGGCTC CCATGGACCC TCTCAAGGGC ATCCTGCTAG GCTTTTTCAC TGGCATCGAG 
GTGGTGATCT GCGCCCTCGT GGTGGTCAGG AAGGACACCA CCTCCCACAC TTACCTGCAA 
TACAGCGGCG TGGTCACTTG GGTGGCTATG ACCACCCAGA TTCTGGCAGC AGGCCTGGGC 
TATGGGCTTC TGGGCGACGG CATAGGCTAC GTGCTTTTTA CACTCTTCGC CACCTACAGC 
ATGCTTCCGC TGCCTCTCAC CTGGGCCATC TTGGCCGGCC TGGGCACATC CTTGCTGCAA 
GTCACACTTC AAGTGCTCAT ACCCAGACTA GCGGTCTTTT CCATCAACCA GGTTCTGGCC 
CAGGTGGTGC TCTTCATGTG CATGAATACA GCAGGCATCT TCATCAGTTA CCTTTCAGAC 
CGCGCCCAGC GGCAGGCCTT CCTGGAGACC CGGAGGTGTG TGGAGGCCAG GCTCCGCCTG 
GAGACAGAGA ACCAAAGACA GGAGCGGCTT GTGCTCTCTG TGCTCCCCAG GTTTGTCGTC 
CTAGAAATGA TCAATGACAT GACCAATGTG GAGGACGAGC ACCTGCAGCA TCAGTTCCAC 
CGCATCTACA TCCATCGCTA CGAGAACGTC AGTATTCTTT TTGCAGATGT CAAAGGATTT 
ACCAACCTCT CTACGACCTT GTCTGCTCAG GAGCTTGTCA GGATGCTCAA CGAGCTCTTT 
GCCAGATTTG ATCGGCTGGC CCATGAGCAT CACTGTCTTC GCATTAAAAT CCTGGGGGAC 
TGCTACTACT GTGTGTCAGG ACTGCCTGAG CCCCGCCAGG ACCATGCTCA TTGCTGTGTT 
GAAATGGGCC TCAGCATGAT CAAAACTATC AGGTTTGTGA GGTCCAGAAC GAAGCATGAT 
GTTGACATGC GAATTGGAAT CCATTCAGGC TCTGTGCTGT GTGGTGTGTT GGGCCTGAGA 
AAATGGCAGT TTGATGTCTG GTCTTGGGAT GTGGACATCG CAAACAAACT TGAATCTGGA 
GGAATCCCTG GGAGAATTCA CATTTCCAAA GCCACACTGG ATTGCCTCAG TGGTGACTAT 
AATGTGGAAG AGGGCCACGG TAAGGAGAGG AATGAATTCT TGAGGAAGCA TAATATAGAG 
ACCTATTTGA TTAAGCAGCC CGAGGAGAGT TTGCTATCCT TGCCTGAAGA TATAGTTAAG 
GAGTCGGTGA GCTGCTCGGA CAGGAGAAAC AGTGGGGCAA CGTTCACAGA AGGATCCTGG 
AGCCCAGAAC TGCCATTCGA CAACATCGTG GGCAAACAGA ATTATTCTCA AATGCGGGAT 
GAAGTATTCA AGTCAAACTT GGTCTGTGCA TTTATCGTTC TTCTGTTTAT CACTGCGATT 
CAAAGTTTGC TTCCTTCTTC GAGGCTGATG CCTATGACCA TCCAGTTCTC CATCCTGATC 
ATGCTGCACT CCGCCCTGGT CCTCATCACC ACGGCAGAAG ACTATAAGTG TCTGCCTCTC 
ATTCTCCGCA AAACCTGTTG TTGGATTAAC GAGACCTATT TGGCCCGCAA CGTCATCATC 
TTTGCTTCCA TCTTGATTAA TTTCCTGGGA GCCGTCATAA ATATTCTGTG GTGTGATTTT 
GACAAGTCGA TACCCTTGAA GAACCTGACT TTCAATTCCT CAGCTGTGTT TACAGATATC 
TGCTCCTACC CAGAGTACTT TGTCTTCACT GGGGTGTTGG CCATGGTGAC GTGTGCAGTA 
TTTCTCCGGC TTAACTCTGT CCTGAAGCTG GCTGTGCTGC TCATTATGAT CGCCATCTAC 
GCCCTGCTGA CAGAGACCAT CTATGCAGGT CTCTTTCTGA GTTATGACAA CCTGAACCAC 
AGTGGAGAAG ATTTTCTGGG GACCAAGGAG GCATCACTGC TACTGATGGC CATGTTCCTT 
CTTGCTGTAT TCTACCATGG ACAGCAGCTG GAGTACACAG CCCGCCTAGA TTTCCTGTGG 
CGAGTACAGG CCAAAGAGGA GATCAACGAG ATGAAGGACT TGAGGGAACA CAATGAGAAC 
ATGCTTCGCA ATATCTTACC CGGCCACGTG GCCCGCCACT TCCTGGAGAA AGACAGAGAC 
AATGAGGAGC TGTATTCTCA ATCCTATGAT GCCGTTGGGG TAATGTTTGC CTCCATTCCT 
GGATTTGCAG ACTTTTACTC TCAGACAGAA ATGAACAACC AGGGAGTGGA ATGTCTGCGC 
TTGCTGAATG AGATCATTGC TGACTTTGAT GAGTTACTTG GAGAGGACCG CTTTCAGGAC 
ATAGAGAAGA TTAAGACCAT TGGTAGTACA TACATGGCTG TCTCAGGACT GTCACCAGAG 
AAACAGCAAT GTGAAGATAA ATGGGGACAT TTGTGTGCCC TGGCTGACTT CTCTCTTGCC 
CTGACTGAAA GCATACAAGA GATCAACAAG CATTCGTTCA ACAATTTTGA ACTCCGTATT 
GGCATCAGCC ATGGCTCAGT GGTAGCAGGT GTAATTGGCG CTAAGAAACC ACAGTATGAC 
ATTTGGGGTA AAACTGTGAA CTTGGCAAGC CGAATGGACA GCACAGGAGT GAGTGGCCGG 
ATCCAAGTTC CTGAGGAGAC CTATCTCATC CTGAAGGATC AGGGCTTTGC CTTCGACTAC 
CGGGGAGAGA TATATGTGAA GGGCATCAGC GAACAAGAAG GGAAAATCAA AACATATTTT 
CTCCTGGGAC GAGTCCAACC CAACCCATTC ATCTTACCCC CAAGAAGACT TCCTGGGCAG 
TACTCTCTGG CTGCAGTTGT CCTTGGCCTT GTCCAGTCTC TCAACAGGCA AAGGCAGAAG 
CAACTACTCA ATGAGAACAG CAACTCTGGC ATCATCAAGA GCCATTACAA CCGGCGGACT 
TTGCTAACGC CAAGTGGGCC AGAGCCTGGA GCACAGGCTG AAGGCACTGA CAAATCCGAT 
TTGCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006241766.1
CDS	1345..4893
Translation	MELSDVHCLSGSEELYTIHPTPPAGDGGSGSRPQRLLWQTAVRHITEQRFIHGHRGGGGGGSRKASNPAGSGPNHHAPQLSSDSVLPLYSLGSGERAHNTGGTKVFPERSGSGSASGSGGGGDLGFLHLDCAPSNSDFFLNGGYSYRGVIFPTLRNSFKSRDLERLYQRYFLGQRRKSEVVMNVLDVLTKLTLLVLHLSLASAPMDPLKGILLGFFTGIEVVICALVVVRKDTTSHTYLQYSGVVTWVAMTTQILAAGLGYGLLGDGIGYVLFTLFATYSMLPLPLTWAILAGLGTSLLQVTLQVLIPRLAVFSINQVLAQVVLFMCMNTAGIFISYLSDRAQRQAFLETRRCVEARLRLETENQRQERLVLSVLPRFVVLEMINDMTNVEDEHLQHQFHRIYIHRYENVSILFADVKGFTNLSTTLSAQELVRMLNELFARFDRLAHEHHCLRIKILGDCYYCVSGLPEPRQDHAHCCVEMGLSMIKTIRFVRSRTKHDVDMRIGIHSGSVLCGVLGLRKWQFDVWSWDVDIANKLESGGIPGRIHISKATLDCLSGDYNVEEGHGKERNEFLRKHNIETYLIKQPEESLLSLPEDIVKESVSCSDRRNSGATFTEGSWSPELPFDNIVGKQNYSQMRDEVFKSNLVCAFIVLLFITAIQSLLPSSRLMPMTIQFSILIMLHSALVLITTAEDYKCLPLILRKTCCWINETYLARNVIIFASILINFLGAVINILWCDFDKSIPLKNLTFNSSAVFTDICSYPEYFVFTGVLAMVTCAVFLRLNSVLKLAVLLIMIAIYALLTETIYAGLFLSYDNLNHSGEDFLGTKEASLLLMAMFLLAVFYHGQQLEYTARLDFLWRVQAKEEINEMKDLREHNENMLRNILPGHVARHFLEKDRDNEELYSQSYDAVGVMFASIPGFADFYSQTEMNNQGVECLRLLNEIIADFDELLGEDRFQDIEKIKTIGSTYMAVSGLSPEKQQCEDKWGHLCALADFSLALTESIQEINKHSFNNFELRIGISHGSVVAGVIGAKKPQYDIWGKTVNLASRMDSTGVSGRIQVPEETYLILKDQGFAFDYRGEIYVKGISEQEGKIKTYFLLGRVQPNPFILPPRRLPGQYSLAAVVLGLVQSLNRQRQKQLLNENSNSGIIKSHYNRRTLLTPSGPEPGAQAEGTDKSDLP

Target ORF information:

RefSeq Version	XM_006241704.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus adenylate cyclase 8 (Adcy8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006241704.3

ORF Insert Sequence:

ATGGAACTCT CGGATGTGCA CTGCCTTAGC GGCAGCGAGG AACTCTATAC CATTCACCCG 
ACGCCCCCGG CCGGCGACGG CGGGAGCGGC TCTCGGCCGC AGCGGCTGCT GTGGCAGACG 
GCGGTGCGGC ACATCACCGA GCAGCGCTTC ATCCACGGCC ACCGAGGCGG CGGCGGCGGG 
GGCTCCCGCA AAGCCTCGAA CCCTGCGGGC AGCGGACCAA ACCACCACGC GCCGCAGCTG 
TCTAGCGACT CGGTGCTGCC TCTCTATTCT CTGGGCTCCG GAGAGCGAGC GCACAACACC 
GGTGGCACCA AAGTCTTTCC GGAACGCAGC GGGAGCGGCA GTGCCAGTGG CAGCGGGGGT 
GGGGGCGACT TGGGCTTCCT GCACCTTGAC TGTGCCCCAA GTAACTCGGA TTTCTTCCTC 
AATGGGGGAT ACAGCTACCG TGGGGTCATT TTCCCAACCC TACGCAACTC CTTCAAGTCT 
CGGGATCTGG AGCGCCTCTA TCAACGCTAT TTCCTGGGCC AGAGGCGCAA ATCGGAGGTA 
GTGATGAACG TGCTGGATGT ACTAACCAAA CTCACCCTTC TAGTCCTTCA CTTGAGCCTA 
GCCTCGGCTC CCATGGACCC TCTCAAGGGC ATCCTGCTAG GCTTTTTCAC TGGCATCGAG 
GTGGTGATCT GCGCCCTCGT GGTGGTCAGG AAGGACACCA CCTCCCACAC TTACCTGCAA 
TACAGCGGCG TGGTCACTTG GGTGGCTATG ACCACCCAGA TTCTGGCAGC AGGCCTGGGC 
TATGGGCTTC TGGGCGACGG CATAGGCTAC GTGCTTTTTA CACTCTTCGC CACCTACAGC 
ATGCTTCCGC TGCCTCTCAC CTGGGCCATC TTGGCCGGCC TGGGCACATC CTTGCTGCAA 
GTCACACTTC AAGTGCTCAT ACCCAGACTA GCGGTCTTTT CCATCAACCA GGTTCTGGCC 
CAGGTGGTGC TCTTCATGTG CATGAATACA GCAGGCATCT TCATCAGTTA CCTTTCAGAC 
CGCGCCCAGC GGCAGGCCTT CCTGGAGACC CGGAGGTGTG TGGAGGCCAG GCTCCGCCTG 
GAGACAGAGA ACCAAAGACA GGAGCGGCTT GTGCTCTCTG TGCTCCCCAG GTTTGTCGTC 
CTAGAAATGA TCAATGACAT GACCAATGTG GAGGACGAGC ACCTGCAGCA TCAGTTCCAC 
CGCATCTACA TCCATCGCTA CGAGAACGTC AGTATTCTTT TTGCAGATGT CAAAGGATTT 
ACCAACCTCT CTACGACCTT GTCTGCTCAG GAGCTTGTCA GGATGCTCAA CGAGCTCTTT 
GCCAGATTTG ATCGGCTGGC CCATGAGCAT CACTGTCTTC GCATTAAAAT CCTGGGGGAC 
TGCTACTACT GTGTGTCAGG ACTGCCTGAG CCCCGCCAGG ACCATGCTCA TTGCTGTGTT 
GAAATGGGCC TCAGCATGAT CAAAACTATC AGGTTTGTGA GGTCCAGAAC GAAGCATGAT 
GTTGACATGC GAATTGGAAT CCATTCAGGC TCTGTGCTGT GTGGTGTGTT GGGCCTGAGA 
AAATGGCAGT TTGATGTCTG GTCTTGGGAT GTGGACATCG CAAACAAACT TGAATCTGGA 
GGAATCCCTG GGAGAATTCA CATTTCCAAA GCCACACTGG ATTGCCTCAG TGGTGACTAT 
AATGTGGAAG AGGGCCACGG TAAGGAGAGG AATGAATTCT TGAGGAAGCA TAATATAGAG 
ACCTATTTGA TTAAGCAGCC CGAGGAGAGT TTGCTATCCT TGCCTGAAGA TATAGTTAAG 
GAGTCGGTGA GCTGCTCGGA CAGGAGAAAC AGTGGGGCAA CGTTCACAGA AGGATCCTGG 
AGCCCAGAAC TGCCATTCGA CAACATCGTG GGCAAACAGA ATTATTCTCA AATGCGGGAT 
GAAGTATTCA AGTCAAACTT GGTCTGTGCA TTTATCGTTC TTCTGTTTAT CACTGCGATT 
CAAAGTTTGC TTCCTTCTTC GAGGCTGATG CCTATGACCA TCCAGTTCTC CATCCTGATC 
ATGCTGCACT CCGCCCTGGT CCTCATCACC ACGGCAGAAG ACTATAAGTG TCTGCCTCTC 
ATTCTCCGCA AAACCTGTTG TTGGATTAAC GAGACCTATT TGGCCCGCAA CGTCATCATC 
TTTGCTTCCA TCTTGATTAA TTTCCTGGGA GCCGTCATAA ATATTCTGTG GTGTGATTTT 
GACAAGTCGA TACCCTTGAA GAACCTGACT TTCAATTCCT CAGCTGTGTT TACAGATATC 
TGCTCCTACC CAGAGTACTT TGTCTTCACT GGGGTGTTGG CCATGGTGAC GTGTGCAGTA 
TTTCTCCGGC TTAACTCTGT CCTGAAGCTG GCTGTGCTGC TCATTATGAT CGCCATCTAC 
GCCCTGCTGA CAGAGACCAT CTATGCAGGT CTCTTTCTGA GTTATGACAA CCTGAACCAC 
AGTGGAGAAG ATTTTCTGGG GACCAAGGAG GCATCACTGC TACTGATGGC CATGTTCCTT 
CTTGCTGTAT TCTACCATGG ACAGCAGCTG GAGTACACAG CCCGCCTAGA TTTCCTGTGG 
CGAGTACAGG CCAAAGAGGA GATCAACGAG ATGAAGGACT TGAGGGAACA CAATGAGAAC 
ATGCTTCGCA ATATCTTACC CGGCCACGTG GCCCGCCACT TCCTGGAGAA AGACAGAGAC 
AATGAGGAGC TGTATTCTCA ATCCTATGAT GCCGTTGGGG TAATGTTTGC CTCCATTCCT 
GGATTTGCAG ACTTTTACTC TCAGACAGAA ATGAACAACC AGGGAGTGGA ATGTCTGCGC 
TTGCTGAATG AGATCATTGC TGACTTTGAT GAGTTACTTG GAGAGGACCG CTTTCAGGAC 
ATAGAGAAGA TTAAGACCAT TGGTAGTACA TACATGGCTG TCTCAGGACT GTCACCAGAG 
AAACAGCAAT GTGAAGATAA ATGGGGACAT TTGTGTGCCC TGGCTGACTT CTCTCTTGCC 
CTGACTGAAA GCATACAAGA GATCAACAAG CATTCGTTCA ACAATTTTGA ACTCCGTATT 
GGCATCAGCC ATGGCTCAGT GGTAGCAGGT GTAATTGGCG CTAAGAAACC ACAGTATGAC 
ATTTGGGGTA AAACTGTGAA CTTGGCAAGC CGAATGGACA GCACAGGAGT GAGTGGCCGG 
ATCCAAGTTC CTGAGGAGAC CTATCTCATC CTGAAGGATC AGGGCTTTGC CTTCGACTAC 
CGGGGAGAGA TATATGTGAA GGGCATCAGC GAACAAGAAG GGAAAATCAA AACATATTTT 
CTCCTGGGAC GAGTCCAACC CAACCCATTC ATCTTACCCC CAAGAAGACT TCCTGGGCAG 
TACTCTCTGG CTGCAGTTGT CCTTGGCCTT GTCCAGTCTC TCAACAGGCA AAGGCAGAAG 
CAACTACTCA ATGAGAACAG CAACTCTGGC ATCATCAAGA GCCATTACAA CCGGCGGACT 
TTGCTAACGC CAAGTGGGCC AGAGCCTGGA GCACAGGCTG AAGGCACTGA CAAATCCGAT 
TTGCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Calcium activated adenylyl cyclase AC8 but not AC1 is required for prolonged behavioral anxiety.
Molecular brain9(1)60(2016 05)
Bernabucci M,Zhuo M

Multilevel control of glucose homeostasis by adenylyl cyclase 8.
Diabetologia58(4)749-57(2015 Apr)
Raoux M,Vacher P,Papin J,Picard A,Kostrzewa E,Devin A,Gaitan J,Limon I,Kas MJ,Magnan C,Lang J

Calcium influx activates adenylyl cyclase 8 for sustained insulin secretion in rat pancreatic beta cells.
Diabetologia58(2)324-33(2015 Feb)
Dou H,Wang C,Wu X,Yao L,Zhang X,Teng S,Xu H,Liu B,Wu Q,Zhang Q,Hu M,Wang Y,Wang L,Wu Y,Shang S,Kang X,Zheng L,Zhang J,Raoux M,Lang J,Li Q,Su J,Yu X,Chen L,Zhou Z

A key phosphorylation site in AC8 mediates regulation of Ca(2+)-dependent cAMP dynamics by an AC8-AKAP79-PKA signalling complex.
Journal of cell science125(Pt 23)5850-9(2012 Dec)
Willoughby D,Halls ML,Everett KL,Ciruela A,Skroblin P,Klussmann E,Cooper DM

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Adcy8 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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