gRNAs
The following Evc2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Evc2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | ORa11828 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001106012.1 | |
| Accession Version | NM_001106012.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3654bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2017-10-01 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | limbin | |
| Comment | Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473963.1. On or before Oct 4, 2007 this sequence version replaced XM_001063003.1, XM_223550.4. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## | |
1 | ATGGGGGCGA CCGGTCTCAC GGGCACTGGG GGACAAGCCA CGTGGGTGCT GGCTGGAAAT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001099482.1 |
| CDS | 59..3712 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_001106012.1 |
1 | ATGGGGGCGA CCGGTCTCAC GGGCACTGGG GGACAAGCCA CGTGGGTGCT GGCTGGAAAT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ORa36158 | |
| Clone ID Related Accession (Same CDS sequence) | XM_006251088.3 , XM_017599114.1 | |
| Accession Version | XM_006251088.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3378bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-07-25 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | limbin isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006251088.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGCATGTGC AGCTGTCAGT GAACAACACC AGGACGCCCA TGTCAGCCAA CCTTTCGGAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_006251150.1 |
| CDS | 196..3573 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_006251088.3 |
1 | ATGCATGTGC AGCTGTCAGT GAACAACACC AGGACGCCCA TGTCAGCCAA CCTTTCGGAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ORa36158 | |
| Clone ID Related Accession (Same CDS sequence) | XM_006251088.3 , XM_017599114.1 | |
| Accession Version | XM_017599114.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3378bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-07-25 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | limbin isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGCATGTGC AGCTGTCAGT GAACAACACC AGGACGCCCA TGTCAGCCAA CCTTTCGGAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_017454603.1 |
| CDS | 239..3616 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_017599114.1 |
1 | ATGCATGTGC AGCTGTCAGT GAACAACACC AGGACGCCCA TGTCAGCCAA CCTTTCGGAC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
EFCAB7 and IQCE regulate hedgehog signaling by tethering the EVC-EVC2 complex to the base of primary cilia. |
Proteomic characterization of the human sperm nucleus. |
Evc2 is a positive modulator of Hedgehog signalling that interacts with Evc at the cilia membrane and is also found in the nucleus. |
Mutations in two nonhomologous genes in a head-to-head configuration cause Ellis-van Creveld syndrome. |
Mutations in a new gene in Ellis-van Creveld syndrome and Weyers acrodental dysostosis. |
