Evc2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Evc2
Entrez Gene ID	68525
Full Name	EvC ciliary complex subunit 2
Synonyms	1110017L09Rik,Lbn,limbin
General protein information	Preferred Names EvC ciliary complex subunit 2 Names limbin Ellis van Creveld syndrome 2 homolog
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 5 Map Location: 5\|5 B3

mRNA and Protein(s)

mRNA	Protein	Name
XM_006504082.3	XP_006504145.1	limbin isoform X1
NM_145920.3	NP_666032.1	limbin precursor
XM_006504082.2	XP_006504145.1	limbin isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	EVC2	NP_667338.3
Pan troglodytes (chimpanzee)	EVC2	XP_001155684.1
Canis lupus familiaris (dog)	EVC2	XP_005618640.1
Bos taurus (cattle)	EVC2	NP_776352.1
Xenopus tropicalis (tropical clawed frog)	evc2	XP_002939100.2
Mus musculus (house mouse)	Evc2	NP_666032.1
Rattus norvegicus (Norway rat)	Evc2	NP_001099482.1
Gallus gallus (chicken)	EVC2	NP_001073217.1
	EVC2	XP_001118862.2

Gene Ontology
Bibliography

Related articles in PubMed

The Role of Ellis-Van Creveld 2(EVC2) in Mice During Cranial Bone Development.
Kwon EK, Louie K, Kulkarni A, Yatabe M, Ruellas ACO, Snider TN, Mochida Y, Cevidanes LHS, Mishina Y, Zhang H
Anatomical record (Hoboken, N.J. : 2007)301(1)46-55(2018 Jan)

Loss of Function of Evc2 in Dental Mesenchyme Leads to Hypomorphic Enamel.
Zhang H, Takeda H, Tsuji T, Kamiya N, Kunieda T, Mochida Y, Mishina Y
Journal of dental research96(4)421-429(2017 Apr)

Elevated Fibroblast Growth Factor Signaling Is Critical for the Pathogenesis of the Dwarfism in Evc2/Limbin Mutant Mice.
Zhang H, Kamiya N, Tsuji T, Takeda H, Scott G, Rajderkar S, Ray MK, Mochida Y, Allen B, Lefebvre V, Hung IH, Ornitz DM, Kunieda T, Mishina Y
PLoS genetics12(12)e1006510(2016 Dec)

Expression of Evc2 in craniofacial tissues and craniofacial bone defects in Evc2 knockout mouse.
Badri MK, Zhang H, Ohyama Y, Venkitapathi S, Alamoudi A, Kamiya N, Takeda H, Ray M, Scott G, Tsuji T, Kunieda T, Mishina Y, Mochida Y
Archives of oral biology68142-52(2016 Aug)

Cilia gene mutations cause atrioventricular septal defects by multiple mechanisms.
Burnicka-Turek O, Steimle JD, Huang W, Felker L, Kamp A, Kweon J, Peterson M, Reeves RH, Maslen CL, Gruber PJ, Yang XH, Shendure J, Moskowitz IP
Human molecular genetics25(14)3011-3028(2016 07)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mutations in Evc2 affect dental mesenchymal stem cell homeostasis, which further leads to hypomorphic enamel formation in mice.
Title: Loss of Function of Evc2 in Dental Mesenchyme Leads to Hypomorphic Enamel.

Elevation of FGF signaling, mainly due to increased Fgf18 expression upon inactivation of Evc2 in the perichondrium, critically contributes to the pathogenesis of limb dwarfism. The limb dwarfism phenotype is partially rescued by inactivation of one allele of Fgf18 in the Evc2 mutant mice
Title: Elevated Fibroblast Growth Factor Signaling Is Critical for the Pathogenesis of the Dwarfism in Evc2/Limbin Mutant Mice.

Loss of EVC2 is associated with facial bone growth defect.
Title: Ellis Van Creveld2 is Required for Postnatal Craniofacial Bone Development.

This study identifies the types of Evc2-expressing cells in craniofacial tissues. Consistent with the expression pattern, abnormal craniofacial bone morphology was found in the Evc2 KO mice, suggesting that EVC2 may be important during craniofacial growth and development.
Title: Expression of Evc2 in craniofacial tissues and craniofacial bone defects in Evc2 knockout mouse.

data reveal that the Hh pathway involves Evc/Evc2-dependent modulations that are necessary for normal endochondral bone formation
Title: The ciliary Evc/Evc2 complex interacts with Smo and controls Hedgehog pathway activity in chondrocytes by regulating Sufu/Gli3 dissociation and Gli3 trafficking in primary cilia.

The following Evc2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Evc2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu12012	NM_145920.3 Latest version!	Mus musculus EvC ciliary complex subunit 2 (Evc2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	16	$643.30 $919.00
OMu35177	XM_006504082.3 Latest version!	Mus musculus EvC ciliary complex subunit 2 (Evc2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OMu35177	XM_006504082.2	Mus musculus Ellis van Creveld syndrome 2 (Evc2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Evc2 ( NM_145920.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_666032.1 Mus musculus EvC ciliary complex subunit 2 (Evc2), mRNA.

Price & Availability↑

CloneID	OMu12012
Clone ID Related Accession (Same CDS sequence)	NM_145920.3
Accession Version	NM_145920.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3663bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-21
Organism	Mus musculus(house mouse)
Product	limbin precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY270131.1, AB083066.1 and AW548747.2. On Aug 15, 2009 this sequence version replaced NM_145920.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC064473.1, SRR1660817.180071.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGGCGA CGGGCCCCAC GGGCGCTGGG GGACGAGCCA CGTGGGTGCT GGCTGGGAAT 
ATCCTGGCCG CTGCCCTGGT ACTGGGCTCG GGTCCGCGTG CACTTCCACC ATCCTTCCCT 
GCCCTGGGCC CAGGGTCACC ATCTCGGCCC GGCCCAGCAG GACCCTGGGC AAGCTCGCAG 
TACAGCGATA TTTCAAGAGA AGCCCGAGGT CCCTTTGAGA ATGGAGTGAT ATTTCAAAAA 
TGCTCACTGG TGTCTGGGCA GAGCGAATCG CAGACCATGC ACGTGCAGCT GTCCGTGAAC 
AACACCAGGA CGCCCACATC AGTCAACCTT TCGAACCTGC TCGTGCTAGA TGAGATCACT 
GGCCTTGCTG TGAAGGAGTC GCCTGGAAAT AATACTCAGG ATGGAATCCA GACTTTCAGA 
AAGAGCTTTC TGCAAGTGGG AGAATGCTAC TCTGTCAGCT ACACAGCCTC GCTGGACCCC 
ACAGCCCTTG GGACTGGGGA GAGCCTGGAT CTTCCTGCCC GGCTCATCTT TCAGAGCCCC 
TCACAGAACA GAACTCAGCT AAAAGCCCCC TTTACCATCA CAGTGGAAGA AAAGATAATG 
GTTCTTCCTA ACCATGGTCT TCACGCAGCA GGCTTCATCG CAGCCTTCCT CATCTCCCTC 
CTCCTGACCG TGGCAGCCCT GTTCTTCTTG GCGAGGGGTC GATGTCTGCA GGGAGGCATG 
CTGTCCAGAT GCCGGATTCA GCATCCTGAG AACAAGCTGG AGCCCTCGCC CTTCACCTCT 
GCTAACGGTG TGAGCCAGGA CCTTTCCCTC AATGACCAAG TGGTTGCCAT CCTGACTTCT 
GAGGAGCCTG GGAGCATGCT TCAAGCCCTA GAAGAGTTGG AGATCGCAAC CCTGAATCAG 
GCAGATGCGG ATCTGGAGGC TTGTCGAAAC CAGATTAGTA AGGACATCAT TGCCCTTTTG 
ATGAAGAATC TGGTCAGCGG TGGTCACCTC TCTCCGCAAA CAGAAAGGAA GATGGCCGCT 
GCTTTCAAAA AGCAGTTTCT GCTGTTGGAA AATGAAATAC AGGAGGAGTA TGAGCGGAAG 
ATGTTGGCAC TGACGGCAGA GTGTGACCTA GAGATGAGGA AGAAGACAGA AAACCAGTAC 
CAGAGGGAGA TGGTGGCCAT GGAGGAAGCA GAAGAGGTGT TGAAGAGAGT TAGCGAGAGG 
TCTGCTGCCG AGTGCAGCAG CCTTTTGCGG ACCCTCCATG GGCTGGAGCA GGAGGACATG 
CAGAGGTCCC TTACTCTGGA CCAAGCCGAG GACTTTGCTC AAGCACACAG GCAGCTGGCT 
GTTTTCCAGA GGAATGAATT ACACAGCATC GTCTATACCC AGATACAGAG TGCTGTCTCC 
AAAGGGGAAC TGAGGCCTGA GGTGGCTAAA ATGATGCTGC AAGACTACTC TAAAACACAG 
GAGAGTGTAG AAGAGCTGAT GGACTTCTTC CAGGCCACCA AGCGATACCA TCTCAGCAAG 
AGGTTTGGCC ACAGAGAGTA CCTGGTCCAG AGACTGCAGG CCATGGAGAC CCGGGTGCAA 
GGGCTGCTGA ACACCGCGGC TACCCAGCTA ACAAGCCTCA TCCATAAACA TGAGAGGGCT 
GGCTACTTGG ATGAAGATCA GATGGAAACG CTATTGGAGC GTGCACAGAC AGAAACCTTT 
TCTATAAAAC AGAAGCTGGA CAATGACTTA AAGCAGGAAA AGAAAAGGCT CCACCAGAGA 
TTAATAACCA GGAGGCGGCG AGAGCTTCTG CAGAAGCACA AGGAGCAGCA GAAGGAGCAG 
GTGTCCCTTG GCGAGGCCTC CAGCACCGCG GAGGACGCAG TCCAGTACCT GCACCAGTGG 
AGGAGCGTGA TGGCCGAGCA CACCGCTGCC CTGGAGGAGC TGCAGGAACG TCTGGACCAG 
GCTGCCCTGG ATGACCTCCG GGTCCTGACT GTGTCGCTGT CGGAGAAAGC CACGGAAGAG 
CTGAGGCGCC TGCAGAGCAC GGCCATGACA CAGGAGCTGC TGAAGCGCAG TGCTCCCTGG 
CTCTTCCTGC AGCAGATCTT AGAGGAGCAC AGCCGGGAGT CTGCGGCTCG CACCACGCAA 
CTGGAGGCAG AGGAGAGGGA ACGTGGCCAG GAGCTGGTGC AGGGCGTGAG GCAGAGGCTA 
CAGCAGGATG CACTGGAGGC CTACACGGAA GAGCAGGCAG AGCTGAGGCA CTGGGAGCAC 
TTGGTGTTCA TGAAACTCTG CTGTGCGGCC ATCTCATTGT CTGAAGAAGA CCTGCTCCGG 
GTAAGGCAGG AGGCACAGGG CTGCTTCAGC CAGCTGGACC GGAGCTTGGC CCTCCCCAGA 
GTCCGTGCAC GAGTGTTGCA GCAGCAGGCG CAGATGGCCT GGCGGGAAGC AGAGTTTCGG 
AAACTGGACC AGGCCCTGGC TGCGCCTGAG CTGCAGTCCA AGGCAAGAAA GCTGCGTTCC 
AAGGGCAGAG GCAAGGCCGA TCTTCTGAAG AAGAACCTGG AGGATAAGAT CCGACTATTC 
GAGGAGCGGG CCCCTGTAGA ACTGGCTGAC CAGGTGCGAG GGGAATTGCT CCAAGAGAGG 
GTCCAGAGGC TGGAGGCCCA GGAGGCGCAC TTTGCTGAGT CACTTGTGGC TCTGCAGTTC 
CAGAAGGTGG CCCGGGCTGC TGAGACCCTC TCAGTCTACA CCGCCCTGCT CAGCATTCAA 
GACCTGCTGC TGGGGGAGCT GAGTGAGTCG GAGACCCTGA CCAAGTCAGC CTGTGTGCAG 
ATCCTGGAGT CCCACAGGCC GGAGCTGCAG GAGCTGCAGG AGCTGGAGAG GAAGCTGGAG 
GACCAGCTGG TGCAGCAGGA AGAGGCCGAG CAGCAGCGGG TCCTGGAGAG CTGGCAGCGG 
TGGGCGGCCG ATGGACCTGG GCTGAGCGAA CCTGAGGAGA TGGATCCTGA AAGGCAGGTC 
TCTGCCATCC TGCGGCAAGC CCTGAACAAG GGCCAGAAGC TGCTGGAGCA GCATCAGCAG 
AGGGTGAGAG AGGAGTGGCA GAACGGTGCG GTGCTGGAAG ATTCTCTGGA AAGCATTGAA 
GCTGACACCA TGGCCAGCCT TTGCAGCCAG GGACTGAGGC TGGTGTCATA CCTCTCAAGG 
ATGACAATGG TACCAGGCAG CACACTACTG CGACTCCTGA GTGTGGTGCT TCCAGCGGCT 
TCACAGCCTC AGCTACTGGC CCTGCTGGAC GCAGTCAGTG AGAAGCACTC CGACCACACA 
GCTGAGAATG AGAGCAGTGG TGAGCAGGCT CAGGCAGAGC AGAGCAAGAG GAGGAAACAC 
CAAGTTTGGT GGAAGGTTTT GGACAGCAGA TTCAGAGCAG ATCTGGTGAG TCAAGGACTG 
GAGAGGATGC TCTGGGCCCG CCAGAAGAAG GAGCGCATAT TGAAGAAGAT ATATGTTCCC 
GTCCAGGAGA GGGTGATGTT TCCTGGGAAA GGAAGCTGGC CACACCTGTC CCTAGAACCC 
ATTGGAGAAC TGGCTCCCAT CCCCATCACT GGGGCAGACG CTATGGACAT ATTAAACACG 
GGAGAAAAGA TCTTTGTATT CAGAAGCCCA AGGGAGCCCG AGATCTCTTT GCGTGTTCCT 
CCCAGGAGGA AAAAGAATTT CCTGAATGCC AAGAAGGCCA ACAGGGCCTT GGGCTTGGAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_666032.1
CDS	71..3733
Translation	MGATGPTGAGGRATWVLAGNILAAALVLGSGPRALPPSFPALGPGSPSRPGPAGPWASSQYSDISREARGPFENGVIFQKCSLVSGQSESQTMHVQLSVNNTRTPTSVNLSNLLVLDEITGLAVKESPGNNTQDGIQTFRKSFLQVGECYSVSYTASLDPTALGTGESLDLPARLIFQSPSQNRTQLKAPFTITVEEKIMVLPNHGLHAAGFIAAFLISLLLTVAALFFLARGRCLQGGMLSRCRIQHPENKLEPSPFTSANGVSQDLSLNDQVVAILTSEEPGSMLQALEELEIATLNQADADLEACRNQISKDIIALLMKNLVSGGHLSPQTERKMAAAFKKQFLLLENEIQEEYERKMLALTAECDLEMRKKTENQYQREMVAMEEAEEVLKRVSERSAAECSSLLRTLHGLEQEDMQRSLTLDQAEDFAQAHRQLAVFQRNELHSIVYTQIQSAVSKGELRPEVAKMMLQDYSKTQESVEELMDFFQATKRYHLSKRFGHREYLVQRLQAMETRVQGLLNTAATQLTSLIHKHERAGYLDEDQMETLLERAQTETFSIKQKLDNDLKQEKKRLHQRLITRRRRELLQKHKEQQKEQVSLGEASSTAEDAVQYLHQWRSVMAEHTAALEELQERLDQAALDDLRVLTVSLSEKATEELRRLQSTAMTQELLKRSAPWLFLQQILEEHSRESAARTTQLEAEERERGQELVQGVRQRLQQDALEAYTEEQAELRHWEHLVFMKLCCAAISLSEEDLLRVRQEAQGCFSQLDRSLALPRVRARVLQQQAQMAWREAEFRKLDQALAAPELQSKARKLRSKGRGKADLLKKNLEDKIRLFEERAPVELADQVRGELLQERVQRLEAQEAHFAESLVALQFQKVARAAETLSVYTALLSIQDLLLGELSESETLTKSACVQILESHRPELQELQELERKLEDQLVQQEEAEQQRVLESWQRWAADGPGLSEPEEMDPERQVSAILRQALNKGQKLLEQHQQRVREEWQNGAVLEDSLESIEADTMASLCSQGLRLVSYLSRMTMVPGSTLLRLLSVVLPAASQPQLLALLDAVSEKHSDHTAENESSGEQAQAEQSKRRKHQVWWKVLDSRFRADLVSQGLERMLWARQKKERILKKIYVPVQERVMFPGKGSWPHLSLEPIGELAPIPITGADAMDILNTGEKIFVFRSPREPEISLRVPPRRKKNFLNAKKANRALGLD

Target ORF information:

RefSeq Version	NM_145920.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus EvC ciliary complex subunit 2 (Evc2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_145920.3

ORF Insert Sequence:

ATGGGGGCGA CGGGCCCCAC GGGCGCTGGG GGACGAGCCA CGTGGGTGCT GGCTGGGAAT 
ATCCTGGCCG CTGCCCTGGT ACTGGGCTCG GGTCCGCGTG CACTTCCACC ATCCTTCCCT 
GCCCTGGGCC CAGGGTCACC ATCTCGGCCC GGCCCAGCAG GACCCTGGGC AAGCTCGCAG 
TACAGCGATA TTTCAAGAGA AGCCCGAGGT CCCTTTGAGA ATGGAGTGAT ATTTCAAAAA 
TGCTCACTGG TGTCTGGGCA GAGCGAATCG CAGACCATGC ACGTGCAGCT GTCCGTGAAC 
AACACCAGGA CGCCCACATC AGTCAACCTT TCGAACCTGC TCGTGCTAGA TGAGATCACT 
GGCCTTGCTG TGAAGGAGTC GCCTGGAAAT AATACTCAGG ATGGAATCCA GACTTTCAGA 
AAGAGCTTTC TGCAAGTGGG AGAATGCTAC TCTGTCAGCT ACACAGCCTC GCTGGACCCC 
ACAGCCCTTG GGACTGGGGA GAGCCTGGAT CTTCCTGCCC GGCTCATCTT TCAGAGCCCC 
TCACAGAACA GAACTCAGCT AAAAGCCCCC TTTACCATCA CAGTGGAAGA AAAGATAATG 
GTTCTTCCTA ACCATGGTCT TCACGCAGCA GGCTTCATCG CAGCCTTCCT CATCTCCCTC 
CTCCTGACCG TGGCAGCCCT GTTCTTCTTG GCGAGGGGTC GATGTCTGCA GGGAGGCATG 
CTGTCCAGAT GCCGGATTCA GCATCCTGAG AACAAGCTGG AGCCCTCGCC CTTCACCTCT 
GCTAACGGTG TGAGCCAGGA CCTTTCCCTC AATGACCAAG TGGTTGCCAT CCTGACTTCT 
GAGGAGCCTG GGAGCATGCT TCAAGCCCTA GAAGAGTTGG AGATCGCAAC CCTGAATCAG 
GCAGATGCGG ATCTGGAGGC TTGTCGAAAC CAGATTAGTA AGGACATCAT TGCCCTTTTG 
ATGAAGAATC TGGTCAGCGG TGGTCACCTC TCTCCGCAAA CAGAAAGGAA GATGGCCGCT 
GCTTTCAAAA AGCAGTTTCT GCTGTTGGAA AATGAAATAC AGGAGGAGTA TGAGCGGAAG 
ATGTTGGCAC TGACGGCAGA GTGTGACCTA GAGATGAGGA AGAAGACAGA AAACCAGTAC 
CAGAGGGAGA TGGTGGCCAT GGAGGAAGCA GAAGAGGTGT TGAAGAGAGT TAGCGAGAGG 
TCTGCTGCCG AGTGCAGCAG CCTTTTGCGG ACCCTCCATG GGCTGGAGCA GGAGGACATG 
CAGAGGTCCC TTACTCTGGA CCAAGCCGAG GACTTTGCTC AAGCACACAG GCAGCTGGCT 
GTTTTCCAGA GGAATGAATT ACACAGCATC GTCTATACCC AGATACAGAG TGCTGTCTCC 
AAAGGGGAAC TGAGGCCTGA GGTGGCTAAA ATGATGCTGC AAGACTACTC TAAAACACAG 
GAGAGTGTAG AAGAGCTGAT GGACTTCTTC CAGGCCACCA AGCGATACCA TCTCAGCAAG 
AGGTTTGGCC ACAGAGAGTA CCTGGTCCAG AGACTGCAGG CCATGGAGAC CCGGGTGCAA 
GGGCTGCTGA ACACCGCGGC TACCCAGCTA ACAAGCCTCA TCCATAAACA TGAGAGGGCT 
GGCTACTTGG ATGAAGATCA GATGGAAACG CTATTGGAGC GTGCACAGAC AGAAACCTTT 
TCTATAAAAC AGAAGCTGGA CAATGACTTA AAGCAGGAAA AGAAAAGGCT CCACCAGAGA 
TTAATAACCA GGAGGCGGCG AGAGCTTCTG CAGAAGCACA AGGAGCAGCA GAAGGAGCAG 
GTGTCCCTTG GCGAGGCCTC CAGCACCGCG GAGGACGCAG TCCAGTACCT GCACCAGTGG 
AGGAGCGTGA TGGCCGAGCA CACCGCTGCC CTGGAGGAGC TGCAGGAACG TCTGGACCAG 
GCTGCCCTGG ATGACCTCCG GGTCCTGACT GTGTCGCTGT CGGAGAAAGC CACGGAAGAG 
CTGAGGCGCC TGCAGAGCAC GGCCATGACA CAGGAGCTGC TGAAGCGCAG TGCTCCCTGG 
CTCTTCCTGC AGCAGATCTT AGAGGAGCAC AGCCGGGAGT CTGCGGCTCG CACCACGCAA 
CTGGAGGCAG AGGAGAGGGA ACGTGGCCAG GAGCTGGTGC AGGGCGTGAG GCAGAGGCTA 
CAGCAGGATG CACTGGAGGC CTACACGGAA GAGCAGGCAG AGCTGAGGCA CTGGGAGCAC 
TTGGTGTTCA TGAAACTCTG CTGTGCGGCC ATCTCATTGT CTGAAGAAGA CCTGCTCCGG 
GTAAGGCAGG AGGCACAGGG CTGCTTCAGC CAGCTGGACC GGAGCTTGGC CCTCCCCAGA 
GTCCGTGCAC GAGTGTTGCA GCAGCAGGCG CAGATGGCCT GGCGGGAAGC AGAGTTTCGG 
AAACTGGACC AGGCCCTGGC TGCGCCTGAG CTGCAGTCCA AGGCAAGAAA GCTGCGTTCC 
AAGGGCAGAG GCAAGGCCGA TCTTCTGAAG AAGAACCTGG AGGATAAGAT CCGACTATTC 
GAGGAGCGGG CCCCTGTAGA ACTGGCTGAC CAGGTGCGAG GGGAATTGCT CCAAGAGAGG 
GTCCAGAGGC TGGAGGCCCA GGAGGCGCAC TTTGCTGAGT CACTTGTGGC TCTGCAGTTC 
CAGAAGGTGG CCCGGGCTGC TGAGACCCTC TCAGTCTACA CCGCCCTGCT CAGCATTCAA 
GACCTGCTGC TGGGGGAGCT GAGTGAGTCG GAGACCCTGA CCAAGTCAGC CTGTGTGCAG 
ATCCTGGAGT CCCACAGGCC GGAGCTGCAG GAGCTGCAGG AGCTGGAGAG GAAGCTGGAG 
GACCAGCTGG TGCAGCAGGA AGAGGCCGAG CAGCAGCGGG TCCTGGAGAG CTGGCAGCGG 
TGGGCGGCCG ATGGACCTGG GCTGAGCGAA CCTGAGGAGA TGGATCCTGA AAGGCAGGTC 
TCTGCCATCC TGCGGCAAGC CCTGAACAAG GGCCAGAAGC TGCTGGAGCA GCATCAGCAG 
AGGGTGAGAG AGGAGTGGCA GAACGGTGCG GTGCTGGAAG ATTCTCTGGA AAGCATTGAA 
GCTGACACCA TGGCCAGCCT TTGCAGCCAG GGACTGAGGC TGGTGTCATA CCTCTCAAGG 
ATGACAATGG TACCAGGCAG CACACTACTG CGACTCCTGA GTGTGGTGCT TCCAGCGGCT 
TCACAGCCTC AGCTACTGGC CCTGCTGGAC GCAGTCAGTG AGAAGCACTC CGACCACACA 
GCTGAGAATG AGAGCAGTGG TGAGCAGGCT CAGGCAGAGC AGAGCAAGAG GAGGAAACAC 
CAAGTTTGGT GGAAGGTTTT GGACAGCAGA TTCAGAGCAG ATCTGGTGAG TCAAGGACTG 
GAGAGGATGC TCTGGGCCCG CCAGAAGAAG GAGCGCATAT TGAAGAAGAT ATATGTTCCC 
GTCCAGGAGA GGGTGATGTT TCCTGGGAAA GGAAGCTGGC CACACCTGTC CCTAGAACCC 
ATTGGAGAAC TGGCTCCCAT CCCCATCACT GGGGCAGACG CTATGGACAT ATTAAACACG 
GGAGAAAAGA TCTTTGTATT CAGAAGCCCA AGGGAGCCCG AGATCTCTTT GCGTGTTCCT 
CCCAGGAGGA AAAAGAATTT CCTGAATGCC AAGAAGGCCA ACAGGGCCTT GGGCTTGGAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Evc2 ( XM_006504082.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006504145.1 Mus musculus EvC ciliary complex subunit 2 (Evc2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu35177
Clone ID Related Accession (Same CDS sequence)	XM_006504082.3 , XM_006504082.2
Accession Version	XM_006504082.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3258bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	limbin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006504082.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATCCA GACTTTCAGA AAGAGCTTTC TGCAAGTGGG AGAATGCTAC TCTGTCAGCT 
ACACAGCCTC GCTGGACCCC ACAGCCCTTG GGACTGGGGA GAGCCTGGAT CTTCCTGCCC 
GGCTCATCTT TCAGAGCCCC TCACAGAACT CAGCTAAAAG CCCCCTTTAC CATCACAGTG 
GAAGAAAAGA TAATGGTTCT TCCTAACCAT GGTCTTCACG CAGCAGGCTT CATCGCAGCC 
TTCCTCATCT CCCTCCTCCT GACCGTGGCA GCCCTGTTCT TCTTGGCGAG GGGTCGATGT 
CTGCAGGGAG GCATGCTGTC CAGATGCCGG ATTCAGCATC CTGAGAACAA GCTGGAGCCC 
TCGCCCTTCA CCTCTGCTAA CGGTGTGAGC CAGGACCTTT CCCTCAATGA CCAAGTGGTT 
GCCATCCTGA CTTCTGAGGA GCCTGGGAGC ATGCTTCAAG CCCTAGAAGA GTTGGAGATC 
GCAACCCTGA ATCAGGCAGA TGCGGATCTG GAGGCTTGTC GAAACCAGAT TAGTAAGGAC 
ATCATTGCCC TTTTGATGAA GAATCTGGTC AGCGGTGGTC ACCTCTCTCC GCAAACAGAA 
AGGAAGATGG CCGCTGCTTT CAAAAAGCAG TTTCTGCTGT TGGAAAATGA AATACAGGAG 
GAGTATGAGC GGAAGATGTT GGCACTGACG GCAGAGTGTG ACCTAGAGAT GAGGAAGAAG 
ACAGAAAACC AGTACCAGAG GGAGATGGTG GCCATGGAGG AAGCAGAAGA GGTGTTGAAG 
AGAGTTAGCG AGAGGTCTGC TGCCGAGTGC AGCAGCCTTT TGCGGACCCT CCATGGGCTG 
GAGCAGGAGG ACATGCAGAG GTCCCTTACT CTGGACCAAG CCGAGGACTT TGCTCAAGCA 
CACAGGCAGC TGGCTGTTTT CCAGAGGAAT GAATTACACA GCATCGTCTA TACCCAGATA 
CAGAGTGCTG TCTCCAAAGG GGAACTGAGG CCTGAGGTGG CTAAAATGAT GCTGCAAGAC 
TACTCTAAAA CACAGGAGAG TGTAGAAGAG CTGATGGACT TCTTCCAGGC CACCAAGCGA 
TACCATCTCA GCAAGAGGTT TGGCCACAGA GAGTACCTGG TCCAGAGACT GCAGGCCATG 
GAGACCCGGG TGCAAGGGCT GCTGAACACC GCGGCTACCC AGCTAACAAG CCTCATCCAT 
AAACATGAGA GGGCTGGCTA CTTGGATGAA GATCAGATGG AAACGCTATT GGAGCGTGCA 
CAGACAGAAA CCTTTTCTAT AAAACAGAAG CTGGACAATG ACTTAAAGCA GGAAAAGAAA 
AGGCTCCACC AGAGATTAAT AACCAGGAGG CGGCGAGAGC TTCTGCAGAA GCACAAGGAG 
CAGCAGAAGG AGCAGGTGTC CCTTGGCGAG GCCTCCAGCA CCGCGGAGGA CGCAGTCCAG 
TACCTGCACC AGTGGAGGAG CGTGATGGCC GAGCACACCG CTGCCCTGGA GGAGCTGCAG 
GAACGTCTGG ACCAGGCTGC CCTGGATGAC CTCCGGGTCC TGACTGTGTC GCTGTCGGAG 
AAAGCCACGG AAGAGCTGAG GCGCCTGCAG AGCACGGCCA TGACACAGGA GCTGCTGAAG 
CGCAGTGCTC CCTGGCTCTT CCTGCAGCAG ATCTTAGAGG AGCACAGCCG GGAGTCTGCG 
GCTCGCACCA CGCAACTGGA GGCAGAGGAG AGGGAACGTG GCCAGGAGCT GGTGCAGGGC 
GTGAGGCAGA GGCTACAGCA GGATGCACTG GAGGCCTACA CGGAAGAGCA GGCAGAGCTG 
AGGCACTGGG AGCACTTGGT GTTCATGAAA CTCTGCTGTG CGGCCATCTC ATTGTCTGAA 
GAAGACCTGC TCCGGGTAAG GCAGGAGGCA CAGGGCTGCT TCAGCCAGCT GGACCGGAGC 
TTGGCCCTCC CCAGAGTCCG TGCACGAGTG TTGCAGCAGC AGGCGCAGAT GGCCTGGCGG 
GAAGCAGAGT TTCGGAAACT GGACCAGGCC CTGGCTGCGC CTGAGCTGCA GTCCAAGGCA 
AGAAAGCTGC GTTCCAAGGG CAGAGGCAAG GCCGATCTTC TGAAGAAGAA CCTGGAGGAT 
AAGATCCGAC TATTCGAGGA GCGGGCCCCT GTAGAACTGG CTGACCAGGT GCGAGGGGAA 
TTGCTCCAAG AGAGGGTCCA GAGGCTGGAG GCCCAGGAGG CGCACTTTGC TGAGTCACTT 
GTGGCTCTGC AGTTCCAGAA GGTGGCCCGG GCTGCTGAGA CCCTCTCAGT CTACACCGCC 
CTGCTCAGCA TTCAAGACCT GCTGCTGGGG GAGCTGAGTG AGTCGGAGAC CCTGACCAAG 
TCAGCCTGTG TGCAGATCCT GGAGTCCCAC AGGCCGGAGC TGCAGGAGCT GCAGGAGCTG 
GAGAGGAAGC TGGAGGACCA GCTGGTGCAG CAGGAAGAGG CCGAGCAGCA GCGGGTCCTG 
GAGAGCTGGC AGCGGTGGGC GGCCGATGGA CCTGGGCTGA GCGAACCTGA GGAGATGGAT 
CCTGAAAGGC AGGTCTCTGC CATCCTGCGG CAAGCCCTGA ACAAGGGCCA GAAGCTGCTG 
GAGCAGCATC AGCAGAGGGT GAGAGAGGAG TGGCAGAACG GTGCGGTGCT GGAAGATTCT 
CTGGAAAGCA TTGAAGCTGA CACCATGGCC AGCCTTTGCA GCCAGGGACT GAGGCTGGTG 
TCATACCTCT CAAGGATGAC AATGGTACCA GGCAGCACAC TACTGCGACT CCTGAGTGTG 
GTGCTTCCAG CGGCTTCACA GCCTCAGCTA CTGGCCCTGC TGGACGCAGT CAGTGAGAAG 
CACTCCGACC ACACAGCTGA GAATGAGAGC AGTGGTGAGC AGGCTCAGGC AGAGCAGAGC 
AAGAGGAGGA AACACCAAGT TTGGTGGAAG GTTTTGGACA GCAGATTCAG AGCAGATCTG 
GTGAGTCAAG GACTGGAGAG GATGCTCTGG GCCCGCCAGA AGAAGGAGCG CATATTGAAG 
AAGATATATG TTCCCGTCCA GGAGAGGGTG ATGTTTCCTG GGAAAGGAAG CTGGCCACAC 
CTGTCCCTAG AACCCATTGG AGAACTGGCT CCCATCCCCA TCACTGGGGC AGACGCTATG 
GACATATTAA ACACGGGAGA AAAGATCTTT GTATTCAGAA GCCCAAGGGA GCCCGAGATC 
TCTTTGCGTG TTCCTCCCAG GAGGAAAAAG AATTTCCTGA ATGCCAAGAA GGCCAACAGG 
GCCTTGGGCT TGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006504145.1
CDS	470..3727
Translation	MESRLSERAFCKWENATLSATQPRWTPQPLGLGRAWIFLPGSSFRAPHRTQLKAPFTITVEEKIMVLPNHGLHAAGFIAAFLISLLLTVAALFFLARGRCLQGGMLSRCRIQHPENKLEPSPFTSANGVSQDLSLNDQVVAILTSEEPGSMLQALEELEIATLNQADADLEACRNQISKDIIALLMKNLVSGGHLSPQTERKMAAAFKKQFLLLENEIQEEYERKMLALTAECDLEMRKKTENQYQREMVAMEEAEEVLKRVSERSAAECSSLLRTLHGLEQEDMQRSLTLDQAEDFAQAHRQLAVFQRNELHSIVYTQIQSAVSKGELRPEVAKMMLQDYSKTQESVEELMDFFQATKRYHLSKRFGHREYLVQRLQAMETRVQGLLNTAATQLTSLIHKHERAGYLDEDQMETLLERAQTETFSIKQKLDNDLKQEKKRLHQRLITRRRRELLQKHKEQQKEQVSLGEASSTAEDAVQYLHQWRSVMAEHTAALEELQERLDQAALDDLRVLTVSLSEKATEELRRLQSTAMTQELLKRSAPWLFLQQILEEHSRESAARTTQLEAEERERGQELVQGVRQRLQQDALEAYTEEQAELRHWEHLVFMKLCCAAISLSEEDLLRVRQEAQGCFSQLDRSLALPRVRARVLQQQAQMAWREAEFRKLDQALAAPELQSKARKLRSKGRGKADLLKKNLEDKIRLFEERAPVELADQVRGELLQERVQRLEAQEAHFAESLVALQFQKVARAAETLSVYTALLSIQDLLLGELSESETLTKSACVQILESHRPELQELQELERKLEDQLVQQEEAEQQRVLESWQRWAADGPGLSEPEEMDPERQVSAILRQALNKGQKLLEQHQQRVREEWQNGAVLEDSLESIEADTMASLCSQGLRLVSYLSRMTMVPGSTLLRLLSVVLPAASQPQLLALLDAVSEKHSDHTAENESSGEQAQAEQSKRRKHQVWWKVLDSRFRADLVSQGLERMLWARQKKERILKKIYVPVQERVMFPGKGSWPHLSLEPIGELAPIPITGADAMDILNTGEKIFVFRSPREPEISLRVPPRRKKNFLNAKKANRALGLD

Target ORF information:

RefSeq Version	XM_006504082.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus EvC ciliary complex subunit 2 (Evc2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006504082.3

ORF Insert Sequence:

ATGGAATCCA GACTTTCAGA AAGAGCTTTC TGCAAGTGGG AGAATGCTAC TCTGTCAGCT 
ACACAGCCTC GCTGGACCCC ACAGCCCTTG GGACTGGGGA GAGCCTGGAT CTTCCTGCCC 
GGCTCATCTT TCAGAGCCCC TCACAGAACT CAGCTAAAAG CCCCCTTTAC CATCACAGTG 
GAAGAAAAGA TAATGGTTCT TCCTAACCAT GGTCTTCACG CAGCAGGCTT CATCGCAGCC 
TTCCTCATCT CCCTCCTCCT GACCGTGGCA GCCCTGTTCT TCTTGGCGAG GGGTCGATGT 
CTGCAGGGAG GCATGCTGTC CAGATGCCGG ATTCAGCATC CTGAGAACAA GCTGGAGCCC 
TCGCCCTTCA CCTCTGCTAA CGGTGTGAGC CAGGACCTTT CCCTCAATGA CCAAGTGGTT 
GCCATCCTGA CTTCTGAGGA GCCTGGGAGC ATGCTTCAAG CCCTAGAAGA GTTGGAGATC 
GCAACCCTGA ATCAGGCAGA TGCGGATCTG GAGGCTTGTC GAAACCAGAT TAGTAAGGAC 
ATCATTGCCC TTTTGATGAA GAATCTGGTC AGCGGTGGTC ACCTCTCTCC GCAAACAGAA 
AGGAAGATGG CCGCTGCTTT CAAAAAGCAG TTTCTGCTGT TGGAAAATGA AATACAGGAG 
GAGTATGAGC GGAAGATGTT GGCACTGACG GCAGAGTGTG ACCTAGAGAT GAGGAAGAAG 
ACAGAAAACC AGTACCAGAG GGAGATGGTG GCCATGGAGG AAGCAGAAGA GGTGTTGAAG 
AGAGTTAGCG AGAGGTCTGC TGCCGAGTGC AGCAGCCTTT TGCGGACCCT CCATGGGCTG 
GAGCAGGAGG ACATGCAGAG GTCCCTTACT CTGGACCAAG CCGAGGACTT TGCTCAAGCA 
CACAGGCAGC TGGCTGTTTT CCAGAGGAAT GAATTACACA GCATCGTCTA TACCCAGATA 
CAGAGTGCTG TCTCCAAAGG GGAACTGAGG CCTGAGGTGG CTAAAATGAT GCTGCAAGAC 
TACTCTAAAA CACAGGAGAG TGTAGAAGAG CTGATGGACT TCTTCCAGGC CACCAAGCGA 
TACCATCTCA GCAAGAGGTT TGGCCACAGA GAGTACCTGG TCCAGAGACT GCAGGCCATG 
GAGACCCGGG TGCAAGGGCT GCTGAACACC GCGGCTACCC AGCTAACAAG CCTCATCCAT 
AAACATGAGA GGGCTGGCTA CTTGGATGAA GATCAGATGG AAACGCTATT GGAGCGTGCA 
CAGACAGAAA CCTTTTCTAT AAAACAGAAG CTGGACAATG ACTTAAAGCA GGAAAAGAAA 
AGGCTCCACC AGAGATTAAT AACCAGGAGG CGGCGAGAGC TTCTGCAGAA GCACAAGGAG 
CAGCAGAAGG AGCAGGTGTC CCTTGGCGAG GCCTCCAGCA CCGCGGAGGA CGCAGTCCAG 
TACCTGCACC AGTGGAGGAG CGTGATGGCC GAGCACACCG CTGCCCTGGA GGAGCTGCAG 
GAACGTCTGG ACCAGGCTGC CCTGGATGAC CTCCGGGTCC TGACTGTGTC GCTGTCGGAG 
AAAGCCACGG AAGAGCTGAG GCGCCTGCAG AGCACGGCCA TGACACAGGA GCTGCTGAAG 
CGCAGTGCTC CCTGGCTCTT CCTGCAGCAG ATCTTAGAGG AGCACAGCCG GGAGTCTGCG 
GCTCGCACCA CGCAACTGGA GGCAGAGGAG AGGGAACGTG GCCAGGAGCT GGTGCAGGGC 
GTGAGGCAGA GGCTACAGCA GGATGCACTG GAGGCCTACA CGGAAGAGCA GGCAGAGCTG 
AGGCACTGGG AGCACTTGGT GTTCATGAAA CTCTGCTGTG CGGCCATCTC ATTGTCTGAA 
GAAGACCTGC TCCGGGTAAG GCAGGAGGCA CAGGGCTGCT TCAGCCAGCT GGACCGGAGC 
TTGGCCCTCC CCAGAGTCCG TGCACGAGTG TTGCAGCAGC AGGCGCAGAT GGCCTGGCGG 
GAAGCAGAGT TTCGGAAACT GGACCAGGCC CTGGCTGCGC CTGAGCTGCA GTCCAAGGCA 
AGAAAGCTGC GTTCCAAGGG CAGAGGCAAG GCCGATCTTC TGAAGAAGAA CCTGGAGGAT 
AAGATCCGAC TATTCGAGGA GCGGGCCCCT GTAGAACTGG CTGACCAGGT GCGAGGGGAA 
TTGCTCCAAG AGAGGGTCCA GAGGCTGGAG GCCCAGGAGG CGCACTTTGC TGAGTCACTT 
GTGGCTCTGC AGTTCCAGAA GGTGGCCCGG GCTGCTGAGA CCCTCTCAGT CTACACCGCC 
CTGCTCAGCA TTCAAGACCT GCTGCTGGGG GAGCTGAGTG AGTCGGAGAC CCTGACCAAG 
TCAGCCTGTG TGCAGATCCT GGAGTCCCAC AGGCCGGAGC TGCAGGAGCT GCAGGAGCTG 
GAGAGGAAGC TGGAGGACCA GCTGGTGCAG CAGGAAGAGG CCGAGCAGCA GCGGGTCCTG 
GAGAGCTGGC AGCGGTGGGC GGCCGATGGA CCTGGGCTGA GCGAACCTGA GGAGATGGAT 
CCTGAAAGGC AGGTCTCTGC CATCCTGCGG CAAGCCCTGA ACAAGGGCCA GAAGCTGCTG 
GAGCAGCATC AGCAGAGGGT GAGAGAGGAG TGGCAGAACG GTGCGGTGCT GGAAGATTCT 
CTGGAAAGCA TTGAAGCTGA CACCATGGCC AGCCTTTGCA GCCAGGGACT GAGGCTGGTG 
TCATACCTCT CAAGGATGAC AATGGTACCA GGCAGCACAC TACTGCGACT CCTGAGTGTG 
GTGCTTCCAG CGGCTTCACA GCCTCAGCTA CTGGCCCTGC TGGACGCAGT CAGTGAGAAG 
CACTCCGACC ACACAGCTGA GAATGAGAGC AGTGGTGAGC AGGCTCAGGC AGAGCAGAGC 
AAGAGGAGGA AACACCAAGT TTGGTGGAAG GTTTTGGACA GCAGATTCAG AGCAGATCTG 
GTGAGTCAAG GACTGGAGAG GATGCTCTGG GCCCGCCAGA AGAAGGAGCG CATATTGAAG 
AAGATATATG TTCCCGTCCA GGAGAGGGTG ATGTTTCCTG GGAAAGGAAG CTGGCCACAC 
CTGTCCCTAG AACCCATTGG AGAACTGGCT CCCATCCCCA TCACTGGGGC AGACGCTATG 
GACATATTAA ACACGGGAGA AAAGATCTTT GTATTCAGAA GCCCAAGGGA GCCCGAGATC 
TCTTTGCGTG TTCCTCCCAG GAGGAAAAAG AATTTCCTGA ATGCCAAGAA GGCCAACAGG 
GCCTTGGGCT TGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Evc2 ( XM_006504082.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006504145.1 Mus musculus Ellis van Creveld syndrome 2 (Evc2), transcript variant X1, mRNA.

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CloneID	OMu35177
Clone ID Related Accession (Same CDS sequence)	XM_006504082.3 , XM_006504082.2
Accession Version	XM_006504082.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3258bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	limbin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000071.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006504082.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAATCCA GACTTTCAGA AAGAGCTTTC TGCAAGTGGG AGAATGCTAC TCTGTCAGCT 
ACACAGCCTC GCTGGACCCC ACAGCCCTTG GGACTGGGGA GAGCCTGGAT CTTCCTGCCC 
GGCTCATCTT TCAGAGCCCC TCACAGAACT CAGCTAAAAG CCCCCTTTAC CATCACAGTG 
GAAGAAAAGA TAATGGTTCT TCCTAACCAT GGTCTTCACG CAGCAGGCTT CATCGCAGCC 
TTCCTCATCT CCCTCCTCCT GACCGTGGCA GCCCTGTTCT TCTTGGCGAG GGGTCGATGT 
CTGCAGGGAG GCATGCTGTC CAGATGCCGG ATTCAGCATC CTGAGAACAA GCTGGAGCCC 
TCGCCCTTCA CCTCTGCTAA CGGTGTGAGC CAGGACCTTT CCCTCAATGA CCAAGTGGTT 
GCCATCCTGA CTTCTGAGGA GCCTGGGAGC ATGCTTCAAG CCCTAGAAGA GTTGGAGATC 
GCAACCCTGA ATCAGGCAGA TGCGGATCTG GAGGCTTGTC GAAACCAGAT TAGTAAGGAC 
ATCATTGCCC TTTTGATGAA GAATCTGGTC AGCGGTGGTC ACCTCTCTCC GCAAACAGAA 
AGGAAGATGG CCGCTGCTTT CAAAAAGCAG TTTCTGCTGT TGGAAAATGA AATACAGGAG 
GAGTATGAGC GGAAGATGTT GGCACTGACG GCAGAGTGTG ACCTAGAGAT GAGGAAGAAG 
ACAGAAAACC AGTACCAGAG GGAGATGGTG GCCATGGAGG AAGCAGAAGA GGTGTTGAAG 
AGAGTTAGCG AGAGGTCTGC TGCCGAGTGC AGCAGCCTTT TGCGGACCCT CCATGGGCTG 
GAGCAGGAGG ACATGCAGAG GTCCCTTACT CTGGACCAAG CCGAGGACTT TGCTCAAGCA 
CACAGGCAGC TGGCTGTTTT CCAGAGGAAT GAATTACACA GCATCGTCTA TACCCAGATA 
CAGAGTGCTG TCTCCAAAGG GGAACTGAGG CCTGAGGTGG CTAAAATGAT GCTGCAAGAC 
TACTCTAAAA CACAGGAGAG TGTAGAAGAG CTGATGGACT TCTTCCAGGC CACCAAGCGA 
TACCATCTCA GCAAGAGGTT TGGCCACAGA GAGTACCTGG TCCAGAGACT GCAGGCCATG 
GAGACCCGGG TGCAAGGGCT GCTGAACACC GCGGCTACCC AGCTAACAAG CCTCATCCAT 
AAACATGAGA GGGCTGGCTA CTTGGATGAA GATCAGATGG AAACGCTATT GGAGCGTGCA 
CAGACAGAAA CCTTTTCTAT AAAACAGAAG CTGGACAATG ACTTAAAGCA GGAAAAGAAA 
AGGCTCCACC AGAGATTAAT AACCAGGAGG CGGCGAGAGC TTCTGCAGAA GCACAAGGAG 
CAGCAGAAGG AGCAGGTGTC CCTTGGCGAG GCCTCCAGCA CCGCGGAGGA CGCAGTCCAG 
TACCTGCACC AGTGGAGGAG CGTGATGGCC GAGCACACCG CTGCCCTGGA GGAGCTGCAG 
GAACGTCTGG ACCAGGCTGC CCTGGATGAC CTCCGGGTCC TGACTGTGTC GCTGTCGGAG 
AAAGCCACGG AAGAGCTGAG GCGCCTGCAG AGCACGGCCA TGACACAGGA GCTGCTGAAG 
CGCAGTGCTC CCTGGCTCTT CCTGCAGCAG ATCTTAGAGG AGCACAGCCG GGAGTCTGCG 
GCTCGCACCA CGCAACTGGA GGCAGAGGAG AGGGAACGTG GCCAGGAGCT GGTGCAGGGC 
GTGAGGCAGA GGCTACAGCA GGATGCACTG GAGGCCTACA CGGAAGAGCA GGCAGAGCTG 
AGGCACTGGG AGCACTTGGT GTTCATGAAA CTCTGCTGTG CGGCCATCTC ATTGTCTGAA 
GAAGACCTGC TCCGGGTAAG GCAGGAGGCA CAGGGCTGCT TCAGCCAGCT GGACCGGAGC 
TTGGCCCTCC CCAGAGTCCG TGCACGAGTG TTGCAGCAGC AGGCGCAGAT GGCCTGGCGG 
GAAGCAGAGT TTCGGAAACT GGACCAGGCC CTGGCTGCGC CTGAGCTGCA GTCCAAGGCA 
AGAAAGCTGC GTTCCAAGGG CAGAGGCAAG GCCGATCTTC TGAAGAAGAA CCTGGAGGAT 
AAGATCCGAC TATTCGAGGA GCGGGCCCCT GTAGAACTGG CTGACCAGGT GCGAGGGGAA 
TTGCTCCAAG AGAGGGTCCA GAGGCTGGAG GCCCAGGAGG CGCACTTTGC TGAGTCACTT 
GTGGCTCTGC AGTTCCAGAA GGTGGCCCGG GCTGCTGAGA CCCTCTCAGT CTACACCGCC 
CTGCTCAGCA TTCAAGACCT GCTGCTGGGG GAGCTGAGTG AGTCGGAGAC CCTGACCAAG 
TCAGCCTGTG TGCAGATCCT GGAGTCCCAC AGGCCGGAGC TGCAGGAGCT GCAGGAGCTG 
GAGAGGAAGC TGGAGGACCA GCTGGTGCAG CAGGAAGAGG CCGAGCAGCA GCGGGTCCTG 
GAGAGCTGGC AGCGGTGGGC GGCCGATGGA CCTGGGCTGA GCGAACCTGA GGAGATGGAT 
CCTGAAAGGC AGGTCTCTGC CATCCTGCGG CAAGCCCTGA ACAAGGGCCA GAAGCTGCTG 
GAGCAGCATC AGCAGAGGGT GAGAGAGGAG TGGCAGAACG GTGCGGTGCT GGAAGATTCT 
CTGGAAAGCA TTGAAGCTGA CACCATGGCC AGCCTTTGCA GCCAGGGACT GAGGCTGGTG 
TCATACCTCT CAAGGATGAC AATGGTACCA GGCAGCACAC TACTGCGACT CCTGAGTGTG 
GTGCTTCCAG CGGCTTCACA GCCTCAGCTA CTGGCCCTGC TGGACGCAGT CAGTGAGAAG 
CACTCCGACC ACACAGCTGA GAATGAGAGC AGTGGTGAGC AGGCTCAGGC AGAGCAGAGC 
AAGAGGAGGA AACACCAAGT TTGGTGGAAG GTTTTGGACA GCAGATTCAG AGCAGATCTG 
GTGAGTCAAG GACTGGAGAG GATGCTCTGG GCCCGCCAGA AGAAGGAGCG CATATTGAAG 
AAGATATATG TTCCCGTCCA GGAGAGGGTG ATGTTTCCTG GGAAAGGAAG CTGGCCACAC 
CTGTCCCTAG AACCCATTGG AGAACTGGCT CCCATCCCCA TCACTGGGGC AGACGCTATG 
GACATATTAA ACACGGGAGA AAAGATCTTT GTATTCAGAA GCCCAAGGGA GCCCGAGATC 
TCTTTGCGTG TTCCTCCCAG GAGGAAAAAG AATTTCCTGA ATGCCAAGAA GGCCAACAGG 
GCCTTGGGCT TGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006504145.1
CDS	448..3705
Translation	MESRLSERAFCKWENATLSATQPRWTPQPLGLGRAWIFLPGSSFRAPHRTQLKAPFTITVEEKIMVLPNHGLHAAGFIAAFLISLLLTVAALFFLARGRCLQGGMLSRCRIQHPENKLEPSPFTSANGVSQDLSLNDQVVAILTSEEPGSMLQALEELEIATLNQADADLEACRNQISKDIIALLMKNLVSGGHLSPQTERKMAAAFKKQFLLLENEIQEEYERKMLALTAECDLEMRKKTENQYQREMVAMEEAEEVLKRVSERSAAECSSLLRTLHGLEQEDMQRSLTLDQAEDFAQAHRQLAVFQRNELHSIVYTQIQSAVSKGELRPEVAKMMLQDYSKTQESVEELMDFFQATKRYHLSKRFGHREYLVQRLQAMETRVQGLLNTAATQLTSLIHKHERAGYLDEDQMETLLERAQTETFSIKQKLDNDLKQEKKRLHQRLITRRRRELLQKHKEQQKEQVSLGEASSTAEDAVQYLHQWRSVMAEHTAALEELQERLDQAALDDLRVLTVSLSEKATEELRRLQSTAMTQELLKRSAPWLFLQQILEEHSRESAARTTQLEAEERERGQELVQGVRQRLQQDALEAYTEEQAELRHWEHLVFMKLCCAAISLSEEDLLRVRQEAQGCFSQLDRSLALPRVRARVLQQQAQMAWREAEFRKLDQALAAPELQSKARKLRSKGRGKADLLKKNLEDKIRLFEERAPVELADQVRGELLQERVQRLEAQEAHFAESLVALQFQKVARAAETLSVYTALLSIQDLLLGELSESETLTKSACVQILESHRPELQELQELERKLEDQLVQQEEAEQQRVLESWQRWAADGPGLSEPEEMDPERQVSAILRQALNKGQKLLEQHQQRVREEWQNGAVLEDSLESIEADTMASLCSQGLRLVSYLSRMTMVPGSTLLRLLSVVLPAASQPQLLALLDAVSEKHSDHTAENESSGEQAQAEQSKRRKHQVWWKVLDSRFRADLVSQGLERMLWARQKKERILKKIYVPVQERVMFPGKGSWPHLSLEPIGELAPIPITGADAMDILNTGEKIFVFRSPREPEISLRVPPRRKKNFLNAKKANRALGLD

Target ORF information:

RefSeq Version	XM_006504082.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus Ellis van Creveld syndrome 2 (Evc2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006504082.2

ORF Insert Sequence:

ATGGAATCCA GACTTTCAGA AAGAGCTTTC TGCAAGTGGG AGAATGCTAC TCTGTCAGCT 
ACACAGCCTC GCTGGACCCC ACAGCCCTTG GGACTGGGGA GAGCCTGGAT CTTCCTGCCC 
GGCTCATCTT TCAGAGCCCC TCACAGAACT CAGCTAAAAG CCCCCTTTAC CATCACAGTG 
GAAGAAAAGA TAATGGTTCT TCCTAACCAT GGTCTTCACG CAGCAGGCTT CATCGCAGCC 
TTCCTCATCT CCCTCCTCCT GACCGTGGCA GCCCTGTTCT TCTTGGCGAG GGGTCGATGT 
CTGCAGGGAG GCATGCTGTC CAGATGCCGG ATTCAGCATC CTGAGAACAA GCTGGAGCCC 
TCGCCCTTCA CCTCTGCTAA CGGTGTGAGC CAGGACCTTT CCCTCAATGA CCAAGTGGTT 
GCCATCCTGA CTTCTGAGGA GCCTGGGAGC ATGCTTCAAG CCCTAGAAGA GTTGGAGATC 
GCAACCCTGA ATCAGGCAGA TGCGGATCTG GAGGCTTGTC GAAACCAGAT TAGTAAGGAC 
ATCATTGCCC TTTTGATGAA GAATCTGGTC AGCGGTGGTC ACCTCTCTCC GCAAACAGAA 
AGGAAGATGG CCGCTGCTTT CAAAAAGCAG TTTCTGCTGT TGGAAAATGA AATACAGGAG 
GAGTATGAGC GGAAGATGTT GGCACTGACG GCAGAGTGTG ACCTAGAGAT GAGGAAGAAG 
ACAGAAAACC AGTACCAGAG GGAGATGGTG GCCATGGAGG AAGCAGAAGA GGTGTTGAAG 
AGAGTTAGCG AGAGGTCTGC TGCCGAGTGC AGCAGCCTTT TGCGGACCCT CCATGGGCTG 
GAGCAGGAGG ACATGCAGAG GTCCCTTACT CTGGACCAAG CCGAGGACTT TGCTCAAGCA 
CACAGGCAGC TGGCTGTTTT CCAGAGGAAT GAATTACACA GCATCGTCTA TACCCAGATA 
CAGAGTGCTG TCTCCAAAGG GGAACTGAGG CCTGAGGTGG CTAAAATGAT GCTGCAAGAC 
TACTCTAAAA CACAGGAGAG TGTAGAAGAG CTGATGGACT TCTTCCAGGC CACCAAGCGA 
TACCATCTCA GCAAGAGGTT TGGCCACAGA GAGTACCTGG TCCAGAGACT GCAGGCCATG 
GAGACCCGGG TGCAAGGGCT GCTGAACACC GCGGCTACCC AGCTAACAAG CCTCATCCAT 
AAACATGAGA GGGCTGGCTA CTTGGATGAA GATCAGATGG AAACGCTATT GGAGCGTGCA 
CAGACAGAAA CCTTTTCTAT AAAACAGAAG CTGGACAATG ACTTAAAGCA GGAAAAGAAA 
AGGCTCCACC AGAGATTAAT AACCAGGAGG CGGCGAGAGC TTCTGCAGAA GCACAAGGAG 
CAGCAGAAGG AGCAGGTGTC CCTTGGCGAG GCCTCCAGCA CCGCGGAGGA CGCAGTCCAG 
TACCTGCACC AGTGGAGGAG CGTGATGGCC GAGCACACCG CTGCCCTGGA GGAGCTGCAG 
GAACGTCTGG ACCAGGCTGC CCTGGATGAC CTCCGGGTCC TGACTGTGTC GCTGTCGGAG 
AAAGCCACGG AAGAGCTGAG GCGCCTGCAG AGCACGGCCA TGACACAGGA GCTGCTGAAG 
CGCAGTGCTC CCTGGCTCTT CCTGCAGCAG ATCTTAGAGG AGCACAGCCG GGAGTCTGCG 
GCTCGCACCA CGCAACTGGA GGCAGAGGAG AGGGAACGTG GCCAGGAGCT GGTGCAGGGC 
GTGAGGCAGA GGCTACAGCA GGATGCACTG GAGGCCTACA CGGAAGAGCA GGCAGAGCTG 
AGGCACTGGG AGCACTTGGT GTTCATGAAA CTCTGCTGTG CGGCCATCTC ATTGTCTGAA 
GAAGACCTGC TCCGGGTAAG GCAGGAGGCA CAGGGCTGCT TCAGCCAGCT GGACCGGAGC 
TTGGCCCTCC CCAGAGTCCG TGCACGAGTG TTGCAGCAGC AGGCGCAGAT GGCCTGGCGG 
GAAGCAGAGT TTCGGAAACT GGACCAGGCC CTGGCTGCGC CTGAGCTGCA GTCCAAGGCA 
AGAAAGCTGC GTTCCAAGGG CAGAGGCAAG GCCGATCTTC TGAAGAAGAA CCTGGAGGAT 
AAGATCCGAC TATTCGAGGA GCGGGCCCCT GTAGAACTGG CTGACCAGGT GCGAGGGGAA 
TTGCTCCAAG AGAGGGTCCA GAGGCTGGAG GCCCAGGAGG CGCACTTTGC TGAGTCACTT 
GTGGCTCTGC AGTTCCAGAA GGTGGCCCGG GCTGCTGAGA CCCTCTCAGT CTACACCGCC 
CTGCTCAGCA TTCAAGACCT GCTGCTGGGG GAGCTGAGTG AGTCGGAGAC CCTGACCAAG 
TCAGCCTGTG TGCAGATCCT GGAGTCCCAC AGGCCGGAGC TGCAGGAGCT GCAGGAGCTG 
GAGAGGAAGC TGGAGGACCA GCTGGTGCAG CAGGAAGAGG CCGAGCAGCA GCGGGTCCTG 
GAGAGCTGGC AGCGGTGGGC GGCCGATGGA CCTGGGCTGA GCGAACCTGA GGAGATGGAT 
CCTGAAAGGC AGGTCTCTGC CATCCTGCGG CAAGCCCTGA ACAAGGGCCA GAAGCTGCTG 
GAGCAGCATC AGCAGAGGGT GAGAGAGGAG TGGCAGAACG GTGCGGTGCT GGAAGATTCT 
CTGGAAAGCA TTGAAGCTGA CACCATGGCC AGCCTTTGCA GCCAGGGACT GAGGCTGGTG 
TCATACCTCT CAAGGATGAC AATGGTACCA GGCAGCACAC TACTGCGACT CCTGAGTGTG 
GTGCTTCCAG CGGCTTCACA GCCTCAGCTA CTGGCCCTGC TGGACGCAGT CAGTGAGAAG 
CACTCCGACC ACACAGCTGA GAATGAGAGC AGTGGTGAGC AGGCTCAGGC AGAGCAGAGC 
AAGAGGAGGA AACACCAAGT TTGGTGGAAG GTTTTGGACA GCAGATTCAG AGCAGATCTG 
GTGAGTCAAG GACTGGAGAG GATGCTCTGG GCCCGCCAGA AGAAGGAGCG CATATTGAAG 
AAGATATATG TTCCCGTCCA GGAGAGGGTG ATGTTTCCTG GGAAAGGAAG CTGGCCACAC 
CTGTCCCTAG AACCCATTGG AGAACTGGCT CCCATCCCCA TCACTGGGGC AGACGCTATG 
GACATATTAA ACACGGGAGA AAAGATCTTT GTATTCAGAA GCCCAAGGGA GCCCGAGATC 
TCTTTGCGTG TTCCTCCCAG GAGGAAAAAG AATTTCCTGA ATGCCAAGAA GGCCAACAGG 
GCCTTGGGCT TGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The Role of Ellis-Van Creveld 2(EVC2) in Mice During Cranial Bone Development.
Anatomical record (Hoboken, N.J. : 2007)301(1)46-55(2018 Jan)
Kwon EK,Louie K,Kulkarni A,Yatabe M,Ruellas ACO,Snider TN,Mochida Y,Cevidanes LHS,Mishina Y,Zhang H

Loss of Function of Evc2 in Dental Mesenchyme Leads to Hypomorphic Enamel.
Journal of dental research96(4)421-429(2017 Apr)
Zhang H,Takeda H,Tsuji T,Kamiya N,Kunieda T,Mochida Y,Mishina Y

Elevated Fibroblast Growth Factor Signaling Is Critical for the Pathogenesis of the Dwarfism in Evc2/Limbin Mutant Mice.
PLoS genetics12(12)e1006510(2016 Dec)
Zhang H,Kamiya N,Tsuji T,Takeda H,Scott G,Rajderkar S,Ray MK,Mochida Y,Allen B,Lefebvre V,Hung IH,Ornitz DM,Kunieda T,Mishina Y

Expression of Evc2 in craniofacial tissues and craniofacial bone defects in Evc2 knockout mouse.
Archives of oral biology68142-52(2016 Aug)
Badri MK,Zhang H,Ohyama Y,Venkitapathi S,Alamoudi A,Kamiya N,Takeda H,Ray M,Scott G,Tsuji T,Kunieda T,Mishina Y,Mochida Y

Cilia gene mutations cause atrioventricular septal defects by multiple mechanisms.
Human molecular genetics25(14)3011-3028(2016 07)
Burnicka-Turek O,Steimle JD,Huang W,Felker L,Kamp A,Kweon J,Peterson M,Reeves RH,Maslen CL,Gruber PJ,Yang XH,Shendure J,Moskowitz IP

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Evc2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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