Nf2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Nf2
Entrez Gene ID	25744
Full Name	neurofibromin 2
Synonyms	merlin
General protein information	Preferred Names neurofibromin 2 Names merlin moesin-ezrin-radixin-like protein neurofibromatosis 2 schwannomin
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 14 Map Location: 14q21
Summary	has similarity to a protein family involved in linking cell membrane proteins to the cytoskeleton; may play a role in negative regulation of cell growth [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006251188.3	XP_006251250.1	merlin isoform X1
NM_013193.1	NP_037325.1	merlin
XM_006251191.3	XP_006251253.1	merlin isoform X2

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	nf2a	NP_001122179.1
	NF2	XP_001106489.1
Bos taurus (cattle)	NF2	XP_003587221.1
Mus musculus (house mouse)	Nf2	NP_035028.2
Rattus norvegicus (Norway rat)	Nf2	NP_037325.1
Canis lupus familiaris (dog)	NF2	XP_534729.2
Gallus gallus (chicken)	NF2	NP_989828.2
Drosophila melanogaster (fruit fly)	Mer	NP_523413.1
Homo sapiens (human)	NF2	NP_000259.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010346	XP_311595.3
Xenopus tropicalis (tropical clawed frog)	nf2	NP_001072362.1

Gene Ontology
Bibliography

Related articles in PubMed

Nf2-Yap signaling controls the expansion of DRG progenitors and glia during DRG development.
Serinagaoglu Y, Par? J, Giovannini M, Cao X
Developmental biology398(1)97-109(2015 Feb)

Tumor suppressor Nf2 limits expansion of the neural progenitor pool by inhibiting Yap/Taz transcriptional coactivators.
Lavado A, He Y, Par? J, Neale G, Olson EN, Giovannini M, Cao X
Development (Cambridge, England)140(16)3323-34(2013 Aug)

Endothelial Cdkn1a (p21) overexpression and accelerated senescence in a mouse model of Fuchs endothelial corneal dystrophy.
Matthaei M, Meng H, Meeker AK, Eberhart CG, Jun AS
Investigative ophthalmology & visual science53(10)6718-27(2012 Sep)

A tight junction-associated Merlin-angiomotin complex mediates Merlin's regulation of mitogenic signaling and tumor suppressive functions.
Yi C, Troutman S, Fera D, Stemmer-Rachamimov A, Avila JL, Christian N, Persson NL, Shimono A, Speicher DW, Marmorstein R, Holmgren L, Kissil JL
Cancer cell19(4)527-40(2011 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results demonstrate that Schwannomin plays an essential role in inducing and/or maintaining the SC's spindle shape, and by stabilizing the bipolar morphology, Sch promotes the alignment of SCs with axons and ultimately influences myelin segment length.
Title: Schwannomin/merlin promotes Schwann cell elongation and influences myelin segment length.

Study demonstrates that Akt-mediated merlin phosphorylation regulates the function of merlin in the absence of an inactivating mutation.
Title: Akt phosphorylation regulates the tumour-suppressor merlin through ubiquitination and degradation.

MYPT-1-PP1delta and its substrate merlin are part of a previously undescribed tumour suppressor cascade that can be hindered in two ways, by mutation of the NF2 gene and by upregulation of the oncoprotein CPI-17
Title: Tumorigenic transformation by CPI-17 through inhibition of a merlin phosphatase.

The data support the significance of regulated Rac signaling in mediating Schwann cell-axon interaction and suggest that controlling Rac activity as a possible therapy for schwannomas.
Title: Temporal control of Rac in Schwann cell-axon interaction is disrupted in NF2-mutant schwannoma cells.

Widespread expression of merlin in brain and its association with protein kinase A suggest a role for merlin in brain biology.
Title: Characterization of the NF2 protein merlin and the ERM protein ezrin in human, rat, and mouse central nervous system.

The following Nf2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nf2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa02292	NM_013193.1 Latest version!	Rattus norvegicus neurofibromin 2 (Nf2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa36227	XM_006251188.3 Latest version!	Rattus norvegicus neurofibromin 2 (Nf2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa36230	XM_006251191.3 Latest version!	Rattus norvegicus neurofibromin 2 (Nf2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Nf2 ( NM_013193.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_037325.1 Rattus norvegicus neurofibromin 2 (Nf2), mRNA.

Price & Availability↑

CloneID	ORa02292
Clone ID Related Accession (Same CDS sequence)	NM_013193.1
Accession Version	NM_013193.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1776bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-21
Organism	Rattus norvegicus(Norway rat)
Product	merlin
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CB578019.1, U61772.1, CB814077.1, BP499772.1, CR467414.1, CO573762.1, CB787451.1, CA511825.1, CK364860.1, CO387057.1, BF558742.1, BQ201846.1 and AA955327.1. On or before Oct 14, 2007 this sequence version replaced XM_341248.3, XM_001067432.1. Sequence Note: This RefSeq record was created from transcript sequence data from multiple strains because no single transcript from the same strain was available for the full length of the gene. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U61772.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGCCGGAG CCATCGCTTC CCGCATGAGC TTCAGCTCAC TCAAGAGGAA GCAGCCCAAG 
ACATTCACGG TGCGGATCGT CACCATGGAT GCCGAGATGG AGTTCAACTG CGAGATGAAG 
TGGAAAGGGA AGGACCTGTT TGATTTGGTA TGCCGGACGC TGGGGCTTCG GGAGACCTGG 
TTCTTTGGAC TGCAGTATAC AATCAAGGAC ACGGTGGCCT GGCTCAAAAT GGACAAGAAG 
GTGTTGGATC ACGATGTTTC GAAGGAAGAA CCAGTTACCT TTCATTTCCT GGCCAAATTT 
TATCCTGAAA ATGCTGAGGA GGAGCTAGTC CAGGAGATCA CGCAGCACTT ATTTTTCTTA 
CAGGTGAAGA AGCAGATTTT GGATGAAAAG GTCTATTGCC CTCCTGAGGC GTCCGTGCTC 
TTGGCGTCAT ATGCTGTTCA GGCCAAGTAT GGCGACTATG ACCCCTCTGT GCACAAGCGA 
GGATTTTTAG CCCAAGAGGA ATTGCTTCCG AAAAGGGTGA TAAATCTCTA TCAGATGACT 
CCGGAAATGT GGGAGGAGAG AATTACTGCT TGGTATGCGG AGCACCGGGG CAGAGCCAGG 
GATGAAGCTG AAATGGAGTA TTTGAAGATA GCTCAGGACT TGGAGATGTA TGGCGTGAAC 
TACTTTACAA TCCGGAATAA AAAGGGCACA GAGTTGCTGC TTGGAGTGGA TGCTCTGGGC 
CTTCACATCT ACGACCCTGA GAACAGGCTG ACCCCCAAGA TCTCCTTCCC GTGGAATGAA 
ATCCGAAACA TCTCCTACAG CGACAAGGAG TTTACTATTA AACCACTGGA TAAGAAAATT 
GATGTCTTTA AATTTAACTC TTCAAAGCTT CGTGTTAATA AGCTGATTCT TCAGCTGTGT 
ATTGGGAACC ATGACCTATT TATGAGGAGA AGGAAAGCTG ACTCTTTAGA AGTTCAGCAG 
ATGAAAGCCC AGGCCAGGGA AGAGAAGGCA AGAAAACAGA TGGAGCGGCA GCGGCTGGCT 
CGAGAAAAGC AGATGCGGGA GGAGGCCGAG CGCACGAGAG ATGAGTTAGA GAGGAGGCTC 
CTGCAGATGA AAGAGGAAGC AACGATGGCC AATGAAGCTC TGATGCGGTC TGAGGAAACA 
GCTGACCTGT TGGCTGAAAA GGCTCAGATC ACAGAAGAGG AGGCCAAGCT TCTGGCTCAA 
AAGGCTGCAG AGGCTGAGCA AGAGATGCAG CGAATCAAGG CCACAGCCAT TCGGACAGAG 
GAGGAGAAGC GCCTGATGGA GCAGAAGGTG CTGGAGGCTG AAGTGCTGGC GTTGAAGATG 
GCTGAGGAGT CAGAGAGGAG GGCCAAGGAG GCTGATCAGT TAAAGCAAGA CTTGCAGGAA 
GCCCGAGAAG CAGAGCGAAG AGCCAAGCAG AAGCTCTTAG AAATCGCCAC CAAGCCCACA 
TATCCACCCA TGAACCCAAT CCCAGCACCA CTGCCTCCTG ACATACCGAG CTTCGACATT 
ATTGGTGACA GCTTGTCATT CGACTTCAAG GACACGGACA TGAAGCGACT TTCCATGGAG 
ATAGAGAAAG AGAAAGTGGA GTACATGGAG AAGAGCAAGC ATCTGCAAGA ACAACTCAAT 
GAACTCAAGA CAGAAATTGA GGCCTTGAAA CTCAAAGAGA GGGAGACAGC CTTGGACATC 
CTACACAGCG AGCACTCAGA CAGAGGCGGC ACCAGCAGCA AGCATAACAC CATCAAAAAG 
CCTCAAGCCC AAGGCAGAAG ACCTATCTGC ATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037325.1
CDS	397..2172
Translation	MAGAIASRMSFSSLKRKQPKTFTVRIVTMDAEMEFNCEMKWKGKDLFDLVCRTLGLRETWFFGLQYTIKDTVAWLKMDKKVLDHDVSKEEPVTFHFLAKFYPENAEEELVQEITQHLFFLQVKKQILDEKVYCPPEASVLLASYAVQAKYGDYDPSVHKRGFLAQEELLPKRVINLYQMTPEMWEERITAWYAEHRGRARDEAEMEYLKIAQDLEMYGVNYFTIRNKKGTELLLGVDALGLHIYDPENRLTPKISFPWNEIRNISYSDKEFTIKPLDKKIDVFKFNSSKLRVNKLILQLCIGNHDLFMRRRKADSLEVQQMKAQAREEKARKQMERQRLAREKQMREEAERTRDELERRLLQMKEEATMANEALMRSEETADLLAEKAQITEEEAKLLAQKAAEAEQEMQRIKATAIRTEEEKRLMEQKVLEAEVLALKMAEESERRAKEADQLKQDLQEAREAERRAKQKLLEIATKPTYPPMNPIPAPLPPDIPSFDIIGDSLSFDFKDTDMKRLSMEIEKEKVEYMEKSKHLQEQLNELKTEIEALKLKERETALDILHSEHSDRGGTSSKHNTIKKPQAQGRRPICI

Target ORF information:

RefSeq Version	NM_013193.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus neurofibromin 2 (Nf2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013193.1

ORF Insert Sequence:

ATGGCCGGAG CCATCGCTTC CCGCATGAGC TTCAGCTCAC TCAAGAGGAA GCAGCCCAAG 
ACATTCACGG TGCGGATCGT CACCATGGAT GCCGAGATGG AGTTCAACTG CGAGATGAAG 
TGGAAAGGGA AGGACCTGTT TGATTTGGTA TGCCGGACGC TGGGGCTTCG GGAGACCTGG 
TTCTTTGGAC TGCAGTATAC AATCAAGGAC ACGGTGGCCT GGCTCAAAAT GGACAAGAAG 
GTGTTGGATC ACGATGTTTC GAAGGAAGAA CCAGTTACCT TTCATTTCCT GGCCAAATTT 
TATCCTGAAA ATGCTGAGGA GGAGCTAGTC CAGGAGATCA CGCAGCACTT ATTTTTCTTA 
CAGGTGAAGA AGCAGATTTT GGATGAAAAG GTCTATTGCC CTCCTGAGGC GTCCGTGCTC 
TTGGCGTCAT ATGCTGTTCA GGCCAAGTAT GGCGACTATG ACCCCTCTGT GCACAAGCGA 
GGATTTTTAG CCCAAGAGGA ATTGCTTCCG AAAAGGGTGA TAAATCTCTA TCAGATGACT 
CCGGAAATGT GGGAGGAGAG AATTACTGCT TGGTATGCGG AGCACCGGGG CAGAGCCAGG 
GATGAAGCTG AAATGGAGTA TTTGAAGATA GCTCAGGACT TGGAGATGTA TGGCGTGAAC 
TACTTTACAA TCCGGAATAA AAAGGGCACA GAGTTGCTGC TTGGAGTGGA TGCTCTGGGC 
CTTCACATCT ACGACCCTGA GAACAGGCTG ACCCCCAAGA TCTCCTTCCC GTGGAATGAA 
ATCCGAAACA TCTCCTACAG CGACAAGGAG TTTACTATTA AACCACTGGA TAAGAAAATT 
GATGTCTTTA AATTTAACTC TTCAAAGCTT CGTGTTAATA AGCTGATTCT TCAGCTGTGT 
ATTGGGAACC ATGACCTATT TATGAGGAGA AGGAAAGCTG ACTCTTTAGA AGTTCAGCAG 
ATGAAAGCCC AGGCCAGGGA AGAGAAGGCA AGAAAACAGA TGGAGCGGCA GCGGCTGGCT 
CGAGAAAAGC AGATGCGGGA GGAGGCCGAG CGCACGAGAG ATGAGTTAGA GAGGAGGCTC 
CTGCAGATGA AAGAGGAAGC AACGATGGCC AATGAAGCTC TGATGCGGTC TGAGGAAACA 
GCTGACCTGT TGGCTGAAAA GGCTCAGATC ACAGAAGAGG AGGCCAAGCT TCTGGCTCAA 
AAGGCTGCAG AGGCTGAGCA AGAGATGCAG CGAATCAAGG CCACAGCCAT TCGGACAGAG 
GAGGAGAAGC GCCTGATGGA GCAGAAGGTG CTGGAGGCTG AAGTGCTGGC GTTGAAGATG 
GCTGAGGAGT CAGAGAGGAG GGCCAAGGAG GCTGATCAGT TAAAGCAAGA CTTGCAGGAA 
GCCCGAGAAG CAGAGCGAAG AGCCAAGCAG AAGCTCTTAG AAATCGCCAC CAAGCCCACA 
TATCCACCCA TGAACCCAAT CCCAGCACCA CTGCCTCCTG ACATACCGAG CTTCGACATT 
ATTGGTGACA GCTTGTCATT CGACTTCAAG GACACGGACA TGAAGCGACT TTCCATGGAG 
ATAGAGAAAG AGAAAGTGGA GTACATGGAG AAGAGCAAGC ATCTGCAAGA ACAACTCAAT 
GAACTCAAGA CAGAAATTGA GGCCTTGAAA CTCAAAGAGA GGGAGACAGC CTTGGACATC 
CTACACAGCG AGCACTCAGA CAGAGGCGGC ACCAGCAGCA AGCATAACAC CATCAAAAAG 
CCTCAAGCCC AAGGCAGAAG ACCTATCTGC ATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nf2 ( XM_006251188.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006251250.1 Rattus norvegicus neurofibromin 2 (Nf2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa36227
Clone ID Related Accession (Same CDS sequence)	XM_006251188.3
Accession Version	XM_006251188.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1791bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	merlin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006251188.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGAG CCATCGCTTC CCGCATGAGC TTCAGCTCAC TCAAGAGGAA GCAGCCCAAG 
ACATTCACGG TGCGGATCGT CACCATGGAT GCCGAGATGG AGTTCAACTG CGAGATGAAG 
TGGAAAGGGA AGGACCTGTT TGATTTGGTA TGCCGGACGC TGGGGCTTCG GGAGACCTGG 
TTCTTTGGAC TGCAGTATAC AATCAAGGAC ACGGTGGCCT GGCTCAAAAT GGACAAGAAG 
GTGTTGGATC ACGATGTTTC GAAGGAAGAA CCAGTTACCT TTCATTTCCT GGCCAAATTT 
TATCCTGAAA ATGCTGAGGA GGAGCTAGTC CAGGAGATCA CGCAGCACTT ATTTTTCTTA 
CAGGTGAAGA AGCAGATTTT GGATGAAAAG GTCTATTGCC CTCCTGAGGC GTCCGTGCTC 
TTGGCGTCAT ATGCTGTTCA GGCCAAGTAT GGCGACTATG ACCCCTCTGT GCACAAGCGA 
GGATTTTTAG CCCAAGAGGA ATTGCTTCCG AAAAGGGTGA TAAATCTCTA TCAGATGACT 
CCGGAAATGT GGGAGGAGAG AATTACTGCT TGGTATGCGG AGCACCGGGG CAGAGCCAGG 
GATGAAGCTG AAATGGAGTA TTTGAAGATA GCTCAGGACT TGGAGATGTA TGGCGTGAAC 
TACTTTACAA TCCGGAATAA AAAGGGCACA GAGTTGCTGC TTGGAGTGGA TGCTCTGGGC 
CTTCACATCT ACGACCCTGA GAACAGGCTG ACCCCCAAGA TCTCCTTCCC GTGGAATGAA 
ATCCGAAACA TCTCCTACAG CGACAAGGAG TTTACTATTA AACCACTGGA TAAGAAAATT 
GATGTCTTTA AATTTAACTC TTCAAAGCTT CGTGTTAATA AGCTGATTCT TCAGCTGTGT 
ATTGGGAACC ATGACCTATT TATGAGGAGA AGGAAAGCTG ACTCTTTAGA AGTTCAGCAG 
ATGAAAGCCC AGGCCAGGGA AGAGAAGGCA AGAAAACAGA TGGAGCGGCA GCGGCTGGCT 
CGAGAAAAGC AGATGCGGGA GGAGGCCGAG CGCACGAGAG ATGAGTTAGA GAGGAGGCTC 
CTGCAGATGA AAGAGGAAGC AACGATGGCC AATGAAGCTC TGATGCGGTC TGAGGAAACA 
GCTGACCTGT TGGCTGAAAA GGCTCAGATC ACAGAAGAGG AGGCCAAGCT TCTGGCTCAA 
AAGGCTGCAG AGGCTGAGCA AGAGATGCAG CGAATCAAGG CCACAGCCAT TCGGACAGAG 
GAGGAGAAGC GCCTGATGGA GCAGAAGGTG CTGGAGGCTG AAGTGCTGGC GTTGAAGATG 
GCTGAGGAGT CAGAGAGGAG GGCCAAGGAG GCTGATCAGT TAAAGCAAGA CTTGCAGGAA 
GCCCGAGAAG CAGAGCGAAG AGCCAAGCAG AAGCTCTTAG AAATCGCCAC CAAGCCCACA 
TATCCACCCA TGAACCCAAT CCCAGCACCA CTGCCTCCTG ACATACCGAG CTTCGACATT 
ATTGGTGACA GCTTGTCATT CGACTTCAAG GACACGGACA TGAAGCGACT TTCCATGGAG 
ATAGAGAAAG AGAAAGTGGA GTACATGGAG AAGAGCAAGC ATCTGCAAGA ACAACTCAAT 
GAACTCAAGA CAGAAATTGA GGCCTTGAAA CTCAAAGAGA GGGAGACAGC CTTGGACATC 
CTACACAGCG AGCACTCAGA CAGAGGCGGC ACCAGCAGCA AGCATAACAC CATCAAAAAG 
CTTACTCTGC AGAGCGCCAA GTCCCGTGTG GCCTTCTTTG AAGAACTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006251250.1
CDS	419..2209
Translation	MAGAIASRMSFSSLKRKQPKTFTVRIVTMDAEMEFNCEMKWKGKDLFDLVCRTLGLRETWFFGLQYTIKDTVAWLKMDKKVLDHDVSKEEPVTFHFLAKFYPENAEEELVQEITQHLFFLQVKKQILDEKVYCPPEASVLLASYAVQAKYGDYDPSVHKRGFLAQEELLPKRVINLYQMTPEMWEERITAWYAEHRGRARDEAEMEYLKIAQDLEMYGVNYFTIRNKKGTELLLGVDALGLHIYDPENRLTPKISFPWNEIRNISYSDKEFTIKPLDKKIDVFKFNSSKLRVNKLILQLCIGNHDLFMRRRKADSLEVQQMKAQAREEKARKQMERQRLAREKQMREEAERTRDELERRLLQMKEEATMANEALMRSEETADLLAEKAQITEEEAKLLAQKAAEAEQEMQRIKATAIRTEEEKRLMEQKVLEAEVLALKMAEESERRAKEADQLKQDLQEAREAERRAKQKLLEIATKPTYPPMNPIPAPLPPDIPSFDIIGDSLSFDFKDTDMKRLSMEIEKEKVEYMEKSKHLQEQLNELKTEIEALKLKERETALDILHSEHSDRGGTSSKHNTIKKLTLQSAKSRVAFFEEL

Target ORF information:

RefSeq Version	XM_006251188.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus neurofibromin 2 (Nf2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006251188.3

ORF Insert Sequence:

ATGGCCGGAG CCATCGCTTC CCGCATGAGC TTCAGCTCAC TCAAGAGGAA GCAGCCCAAG 
ACATTCACGG TGCGGATCGT CACCATGGAT GCCGAGATGG AGTTCAACTG CGAGATGAAG 
TGGAAAGGGA AGGACCTGTT TGATTTGGTA TGCCGGACGC TGGGGCTTCG GGAGACCTGG 
TTCTTTGGAC TGCAGTATAC AATCAAGGAC ACGGTGGCCT GGCTCAAAAT GGACAAGAAG 
GTGTTGGATC ACGATGTTTC GAAGGAAGAA CCAGTTACCT TTCATTTCCT GGCCAAATTT 
TATCCTGAAA ATGCTGAGGA GGAGCTAGTC CAGGAGATCA CGCAGCACTT ATTTTTCTTA 
CAGGTGAAGA AGCAGATTTT GGATGAAAAG GTCTATTGCC CTCCTGAGGC GTCCGTGCTC 
TTGGCGTCAT ATGCTGTTCA GGCCAAGTAT GGCGACTATG ACCCCTCTGT GCACAAGCGA 
GGATTTTTAG CCCAAGAGGA ATTGCTTCCG AAAAGGGTGA TAAATCTCTA TCAGATGACT 
CCGGAAATGT GGGAGGAGAG AATTACTGCT TGGTATGCGG AGCACCGGGG CAGAGCCAGG 
GATGAAGCTG AAATGGAGTA TTTGAAGATA GCTCAGGACT TGGAGATGTA TGGCGTGAAC 
TACTTTACAA TCCGGAATAA AAAGGGCACA GAGTTGCTGC TTGGAGTGGA TGCTCTGGGC 
CTTCACATCT ACGACCCTGA GAACAGGCTG ACCCCCAAGA TCTCCTTCCC GTGGAATGAA 
ATCCGAAACA TCTCCTACAG CGACAAGGAG TTTACTATTA AACCACTGGA TAAGAAAATT 
GATGTCTTTA AATTTAACTC TTCAAAGCTT CGTGTTAATA AGCTGATTCT TCAGCTGTGT 
ATTGGGAACC ATGACCTATT TATGAGGAGA AGGAAAGCTG ACTCTTTAGA AGTTCAGCAG 
ATGAAAGCCC AGGCCAGGGA AGAGAAGGCA AGAAAACAGA TGGAGCGGCA GCGGCTGGCT 
CGAGAAAAGC AGATGCGGGA GGAGGCCGAG CGCACGAGAG ATGAGTTAGA GAGGAGGCTC 
CTGCAGATGA AAGAGGAAGC AACGATGGCC AATGAAGCTC TGATGCGGTC TGAGGAAACA 
GCTGACCTGT TGGCTGAAAA GGCTCAGATC ACAGAAGAGG AGGCCAAGCT TCTGGCTCAA 
AAGGCTGCAG AGGCTGAGCA AGAGATGCAG CGAATCAAGG CCACAGCCAT TCGGACAGAG 
GAGGAGAAGC GCCTGATGGA GCAGAAGGTG CTGGAGGCTG AAGTGCTGGC GTTGAAGATG 
GCTGAGGAGT CAGAGAGGAG GGCCAAGGAG GCTGATCAGT TAAAGCAAGA CTTGCAGGAA 
GCCCGAGAAG CAGAGCGAAG AGCCAAGCAG AAGCTCTTAG AAATCGCCAC CAAGCCCACA 
TATCCACCCA TGAACCCAAT CCCAGCACCA CTGCCTCCTG ACATACCGAG CTTCGACATT 
ATTGGTGACA GCTTGTCATT CGACTTCAAG GACACGGACA TGAAGCGACT TTCCATGGAG 
ATAGAGAAAG AGAAAGTGGA GTACATGGAG AAGAGCAAGC ATCTGCAAGA ACAACTCAAT 
GAACTCAAGA CAGAAATTGA GGCCTTGAAA CTCAAAGAGA GGGAGACAGC CTTGGACATC 
CTACACAGCG AGCACTCAGA CAGAGGCGGC ACCAGCAGCA AGCATAACAC CATCAAAAAG 
CTTACTCTGC AGAGCGCCAA GTCCCGTGTG GCCTTCTTTG AAGAACTCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nf2 ( XM_006251191.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006251253.1 Rattus norvegicus neurofibromin 2 (Nf2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa36230
Clone ID Related Accession (Same CDS sequence)	XM_006251191.3
Accession Version	XM_006251191.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1500bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	merlin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006251191.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGGAG CCATCGCTTC CCGCATGAGC TTCAGCTCAC TCAAGAGGAA GCAGCCCAAG 
ACATTCACGG TGCGGATCGT CACCATGGAT GCCGAGATGG AGTTCAACTG CGAGATGAAG 
TGGAAAGGGA AGGACCTGTT TGATTTGGTA TGCCGGACGC TGGGGCTTCG GGAGACCTGG 
TTCTTTGGAC TGCAGTATAC AATCAAGGAC ACGGTGGCCT GGCTCAAAAT GGACAAGAAG 
GTGTTGGATC ACGATGTTTC GAAGGAAGAA CCAGTTACCT TTCATTTCCT GGCCAAATTT 
TATCCTGAAA ATGCTGAGGA GGAGCTAGTC CAGGAGATCA CGCAGCACTT ATTTTTCTTA 
CAGGTGAAGA AGCAGATTTT GGATGAAAAG GTCTATTGCC CTCCTGAGGC GTCCGTGCTC 
TTGGCGTCAT ATGCTGTTCA GGCCAAGTAT GGCGACTATG ACCCCTCTGT GCACAAGCGA 
GGATTTTTAG CCCAAGAGGA ATTGCTTCCG AAAAGGGTGA TAAATCTCTA TCAGATGACT 
CCGGAAATGT GGGAGGAGAG AATTACTGCT TGGTATGCGG AGCACCGGGG CAGAGCCAGG 
GATGAAGCTG AAATGGAGTA TTTGAAGATA GCTCAGGACT TGGAGATGTA TGGCGTGAAC 
TACTTTACAA TCCGGAATAA AAAGGGCACA GAGTTGCTGC TTGGAGTGGA TGCTCTGGGC 
CTTCACATCT ACGACCCTGA GAACAGGCTG ACCCCCAAGA TCTCCTTCCC GTGGAATGAA 
ATCCGAAACA TCTCCTACAG CGACAAGGAG TTTACTATTA AACCACTGGA TAAGAAAATT 
GATGTCTTTA AATTTAACTC TTCAAAGCTT CGTGTTAATA AGCTGATTCT TCAGCTGTGT 
ATTGGGAACC ATGACCTATT TATGAGGAGA AGGAAAGCTG ACTCTTTAGA AGTTCAGCAG 
ATGAAAGCCC AGGCCAGGGA AGAGAAGGCA AGAAAACAGA TGGAGCGGCA GCGGCTGGCT 
CGAGAAAAGC AGATGCGGGA GGAGGCCGAG CGCACGAGAG ATGAGTTAGA GAGGAGGCTC 
CTGCAGATGA AAGAGGAAGC AACGATGGCC AATGAAGCTC TGATGCGGTC TGAGGAAACA 
GCTGACCTGT TGGCTGAAAA GGCTCAGATC ACAGAAGAGG AGGCCAAGCT TCTGGCTCAA 
AAGGCTGCAG AGGCTGAGCA AGAGATGCAG CGAATCAAGG CCACAGCCAT TCGGACAGAG 
GAGGAGAAGC GCCTGATGGA GCAGAAGGTG CTGGAGGCTG AAGTGCTGGC GTTGAAGATG 
GCTGAGGAGT CAGAGAGGAG GGCCAAGGAG GCTGATCAGT TAAAGCAAGA CTTGCAGGAA 
GCCCGAGAAG CAGAGCGAAG AGCCAAGCAG AAGCTCTTAG AAATCGCCAC CAAGCCCACA 
TATCCAATAT GCACAGGTGC CCAGACAGCC CAGCCTTGGA GGCAGAAAAG CTTGGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006251253.1
CDS	421..1920
Translation	MAGAIASRMSFSSLKRKQPKTFTVRIVTMDAEMEFNCEMKWKGKDLFDLVCRTLGLRETWFFGLQYTIKDTVAWLKMDKKVLDHDVSKEEPVTFHFLAKFYPENAEEELVQEITQHLFFLQVKKQILDEKVYCPPEASVLLASYAVQAKYGDYDPSVHKRGFLAQEELLPKRVINLYQMTPEMWEERITAWYAEHRGRARDEAEMEYLKIAQDLEMYGVNYFTIRNKKGTELLLGVDALGLHIYDPENRLTPKISFPWNEIRNISYSDKEFTIKPLDKKIDVFKFNSSKLRVNKLILQLCIGNHDLFMRRRKADSLEVQQMKAQAREEKARKQMERQRLAREKQMREEAERTRDELERRLLQMKEEATMANEALMRSEETADLLAEKAQITEEEAKLLAQKAAEAEQEMQRIKATAIRTEEEKRLMEQKVLEAEVLALKMAEESERRAKEADQLKQDLQEAREAERRAKQKLLEIATKPTYPICTGAQTAQPWRQKSLA

Target ORF information:

RefSeq Version	XM_006251191.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus neurofibromin 2 (Nf2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006251191.3

ORF Insert Sequence:

ATGGCCGGAG CCATCGCTTC CCGCATGAGC TTCAGCTCAC TCAAGAGGAA GCAGCCCAAG 
ACATTCACGG TGCGGATCGT CACCATGGAT GCCGAGATGG AGTTCAACTG CGAGATGAAG 
TGGAAAGGGA AGGACCTGTT TGATTTGGTA TGCCGGACGC TGGGGCTTCG GGAGACCTGG 
TTCTTTGGAC TGCAGTATAC AATCAAGGAC ACGGTGGCCT GGCTCAAAAT GGACAAGAAG 
GTGTTGGATC ACGATGTTTC GAAGGAAGAA CCAGTTACCT TTCATTTCCT GGCCAAATTT 
TATCCTGAAA ATGCTGAGGA GGAGCTAGTC CAGGAGATCA CGCAGCACTT ATTTTTCTTA 
CAGGTGAAGA AGCAGATTTT GGATGAAAAG GTCTATTGCC CTCCTGAGGC GTCCGTGCTC 
TTGGCGTCAT ATGCTGTTCA GGCCAAGTAT GGCGACTATG ACCCCTCTGT GCACAAGCGA 
GGATTTTTAG CCCAAGAGGA ATTGCTTCCG AAAAGGGTGA TAAATCTCTA TCAGATGACT 
CCGGAAATGT GGGAGGAGAG AATTACTGCT TGGTATGCGG AGCACCGGGG CAGAGCCAGG 
GATGAAGCTG AAATGGAGTA TTTGAAGATA GCTCAGGACT TGGAGATGTA TGGCGTGAAC 
TACTTTACAA TCCGGAATAA AAAGGGCACA GAGTTGCTGC TTGGAGTGGA TGCTCTGGGC 
CTTCACATCT ACGACCCTGA GAACAGGCTG ACCCCCAAGA TCTCCTTCCC GTGGAATGAA 
ATCCGAAACA TCTCCTACAG CGACAAGGAG TTTACTATTA AACCACTGGA TAAGAAAATT 
GATGTCTTTA AATTTAACTC TTCAAAGCTT CGTGTTAATA AGCTGATTCT TCAGCTGTGT 
ATTGGGAACC ATGACCTATT TATGAGGAGA AGGAAAGCTG ACTCTTTAGA AGTTCAGCAG 
ATGAAAGCCC AGGCCAGGGA AGAGAAGGCA AGAAAACAGA TGGAGCGGCA GCGGCTGGCT 
CGAGAAAAGC AGATGCGGGA GGAGGCCGAG CGCACGAGAG ATGAGTTAGA GAGGAGGCTC 
CTGCAGATGA AAGAGGAAGC AACGATGGCC AATGAAGCTC TGATGCGGTC TGAGGAAACA 
GCTGACCTGT TGGCTGAAAA GGCTCAGATC ACAGAAGAGG AGGCCAAGCT TCTGGCTCAA 
AAGGCTGCAG AGGCTGAGCA AGAGATGCAG CGAATCAAGG CCACAGCCAT TCGGACAGAG 
GAGGAGAAGC GCCTGATGGA GCAGAAGGTG CTGGAGGCTG AAGTGCTGGC GTTGAAGATG 
GCTGAGGAGT CAGAGAGGAG GGCCAAGGAG GCTGATCAGT TAAAGCAAGA CTTGCAGGAA 
GCCCGAGAAG CAGAGCGAAG AGCCAAGCAG AAGCTCTTAG AAATCGCCAC CAAGCCCACA 
TATCCAATAT GCACAGGTGC CCAGACAGCC CAGCCTTGGA GGCAGAAAAG CTTGGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Nf2-Yap signaling controls the expansion of DRG progenitors and glia during DRG development.
Developmental biology398(1)97-109(2015 Feb)
Serinagaoglu Y,Par? J,Giovannini M,Cao X

Tumor suppressor Nf2 limits expansion of the neural progenitor pool by inhibiting Yap/Taz transcriptional coactivators.
Development (Cambridge, England)140(16)3323-34(2013 Aug)
Lavado A,He Y,Par? J,Neale G,Olson EN,Giovannini M,Cao X

Endothelial Cdkn1a (p21) overexpression and accelerated senescence in a mouse model of Fuchs endothelial corneal dystrophy.
Investigative ophthalmology & visual science53(10)6718-27(2012 Sep)
Matthaei M,Meng H,Meeker AK,Eberhart CG,Jun AS

A tight junction-associated Merlin-angiomotin complex mediates Merlin's regulation of mitogenic signaling and tumor suppressive functions.
Cancer cell19(4)527-40(2011 Apr)
Yi C,Troutman S,Fera D,Stemmer-Rachamimov A,Avila JL,Christian N,Persson NL,Shimono A,Speicher DW,Marmorstein R,Holmgren L,Kissil JL

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Nf2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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