Mer cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mer
Entrez Gene ID	32979
Full Name	Merlin
Synonyms	BG01543,CG14228,D-Mer,Dmel\CG14228,Dmerlin,EMR2,Emr2,NF2,mer
General protein information	Preferred Names Merlin Names CG14228 gene product from transcript CG14228-RB BG01543 CG14228-PA CG14228-PB D merlin Ezrin-moesin-radixin-2 Mer-PA Mer-PB Merlin/Neurofibromin 2 dmerlin merlin
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: X Map Location: 18E1-18E1

mRNA and Protein(s)

mRNA	Protein	Name
NM_078689.4	NP_523413.1	merlin, isoform A
NM_001298529.1	NP_001285458.1	merlin, isoform B

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	nf2a	NP_001122179.1
	NF2	XP_001106489.1
Bos taurus (cattle)	NF2	XP_003587221.1
Mus musculus (house mouse)	Nf2	NP_035028.2
Rattus norvegicus (Norway rat)	Nf2	NP_037325.1
Canis lupus familiaris (dog)	NF2	XP_534729.2
Gallus gallus (chicken)	NF2	NP_989828.2
Drosophila melanogaster (fruit fly)	Mer	NP_523413.1
Homo sapiens (human)	NF2	NP_000259.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010346	XP_311595.3
Xenopus tropicalis (tropical clawed frog)	nf2	NP_001072362.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Merlin and Kibra activate Hippo signaling in parallel to Expanded at a spatially distinct cellular domain, the medial apical cortex.
Title: Kibra and Merlin Activate the Hippo Pathway Spatially Distinct from and Independent of Expanded.

Rae1 increases activation of the core kinases Hippo and Warts and plays a post-transcriptional role to increase the protein levels of the Merlin, Hippo, and Warts components of the pathway; therefore, in addition to Rae1 coordinating organ size regulation with proliferative control, we propose that Rae1 also acts in a feedback circuit to regulate pathway homeostas
Title: The Hippo Pathway Targets Rae1 to Regulate Mitosis and Organ Size and to Feed Back to Regulate Upstream Components Merlin, Hippo, and Warts.

Sip1 facilitates the activation of Merlin as a tumor suppressor protein.
Title: Regulation of cell proliferation and adhesion by means of a novel region of drosophila merlin interacting with Sip1.

These results indicate that in both Drosophila and mammals, Merlin activates Wts/Lats phosphorylation without stimulating the intrinsic kinase activity of Hpo/Mst.
Title: Spatial organization of Hippo signaling at the plasma membrane mediated by the tumor suppressor Merlin/NF2.

Drosophila melanogaster protein phosphatase type 1 beta (flapwing) co-regulates dephosphorylation and altered activity of both Merlin and Moesin.
Title: The PP1 phosphatase flapwing regulates the activity of Merlin and Moesin in Drosophila.

The following Mer gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mer cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa02167	NM_078689.4 Latest version!	Drosophila melanogaster merlin, transcript variant A (Mer), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa02167	NM_001298529.1 Latest version!	Drosophila melanogaster merlin, transcript variant B (Mer), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mer ( NM_078689.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_523413.1 Drosophila melanogaster merlin, transcript variant A (Mer), mRNA.

Price & Availability↑

CloneID	OFa02167
Clone ID Related Accession (Same CDS sequence)	NM_078689.4 , NM_001298529.1
Accession Version	NM_078689.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1908bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	merlin, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_078689.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCCCCT TCGGCTCCAA GAAAAACCGA TCCCTGTCGG TGCGGGTGAG CACCTTCGAC 
TCGGAGCTGG AGTTCAAGCT GGAGCCGCGC GCCTCCGGCC AGGATCTGTT CGACCTGGTG 
TGCCGCACCA TCGGGCTGCG CGAGTCTTGG TACTTTGGGC TGCAGTACGT CGACACCCGC 
AGCAACGTGT CCTGGCTGAA AATGGAGAAG CGGGTGCGCG ACCAGCGCGT TGAGCTGCAC 
GCTAGCAATA ATGTGTATGT GTTTAGCTTC TACGCCAAGT TCTTTCCGGA GAACGTCAGC 
GAGGAGCTGA TCCAGGAGAT CACGCAGCAC CTGTTCTTCC TGCAGGTCAA GCAGAGCATC 
CTCTCCATGG ACATCTACTG CCGCCCGGAG GCGTCCGTGC TGCTGGCCTC CTACGCGGTG 
CACGTGCAAT ACGGTCCGTA CGACTACGAG ACCTACAAGG ACGGTATGCT GGCCGGGGGT 
GAGCTTCTGC CCAAGGGAGT GACCGATCAG TACCAGATGA CGCCCGAAAT GTGGGAGGAA 
CGGATCAAGA CATGGTACAT GGACCATGAG CCCATGACGC GCGACGAGGT GGAGATGGAG 
TACCTGAAGA TTGCTCAGGA CCTGGACATG TACGGAGTAA ACTACTTTCC TATTACGAAT 
AAGAACAAAA CCAAATTGTG GCTGGGCGTC ACCTCCGTGG GCCTAAACAT CTACGATGAG 
CGCGATAAGC TAACGCCAAA GACAACGTTC CAGTGGAACG AGATACGGCA CGTCAGCTTC 
GACGACAAGA AGTTCACCAT TCGCCTGGTG GATGCCAAGG TTTCGAATTT CATCTTTTAC 
TCGCAGGACC TCCACATCAA CAAAATGATT CTTGACCTGT GCAAGGGCAA TCACGATCTA 
TATATGCGCC GCCGCAAACC CGACACCATG GAAATCCAGC AGATGAAGGC CCAAGCCAAG 
GAGGAGAAGC AACGCCGCCA GATCGAACGC AAAAAGTTCA TCAGGGAAAA GAAGCTGCGC 
GAAAAGGCGG AGCACGAGCG CTACGAGCTG GAGAAGAGCA TGGAGCACCT GCAAAACGAA 
ATGCGCATGG CCAACGATGC ACTTCGGCGT TCCGAAGAGA CCAAGGAGCT GTACTTCGAA 
AAGAGTCGTG TCAACGAGGA GCAGATGCAG CTGACCGAGT GCAAGGCAAA CCACTTCAAG 
ACGGAAATGG ACCGGCTGCG CGAGCGACAA ATGAAAATCG AGCGGGAGAA GCACGACCTG 
GAGAAGAAGA TTCGCGACGC CGACTTCTAT GTCCACCAGC TGACGGTGGA GAACGACAAG 
CGGGAGGCCG AGACTGAGAA GCTGCGCAAG GAGCTGATCT GCGCCAAAAT GGCGGAGCGC 
GAGGCCACCG CTCGCCTGCT CGAATTCCTC AACAGCGGCC GCAAGTCCTC CACGGATAGC 
CTGCTGACCG CCTCCAGTGT GTCGCACGCG GCGAATACGG CGTCCAGCAT GGCGGCCATC 
AGCACACCCT CCCTGATCAC AAGCAGCTCA ACCAATGATT TGGAGACCGC CGGCGGCGCC 
GAGCTGACCA CCCACTCGTC ACACTACCTG GTCCAAGGTG ACAACTCCAG CGGCATTTCG 
GACGACTTCG AGCCCAAGGA GTTCATACTC ACCGACAACG AGATGGAGCA GATCACCAAT 
GAGATGGAGC GCAACCACCT CGACTACCTG CGCAACTCCA AGCAGGTGCA AAGTCAACTG 
CAGACACTGC GCTCCGAGAT CGCGCCGCAC AAGATTGAGG AGAACCAGAG CAACCTGGAC 
ATCCTCAGCG AGGCGCAGAT CAAGGCCGGC GAAAATAAAT ACTCCACGCT CAAGAAACTC 
AAGTCCGGCT CGACCAAGGC GCGTGTCGCC TTCTTCGAGG AGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_523413.1
CDS	358..2265
Translation	MSPFGSKKNRSLSVRVSTFDSELEFKLEPRASGQDLFDLVCRTIGLRESWYFGLQYVDTRSNVSWLKMEKRVRDQRVELHASNNVYVFSFYAKFFPENVSEELIQEITQHLFFLQVKQSILSMDIYCRPEASVLLASYAVHVQYGPYDYETYKDGMLAGGELLPKGVTDQYQMTPEMWEERIKTWYMDHEPMTRDEVEMEYLKIAQDLDMYGVNYFPITNKNKTKLWLGVTSVGLNIYDERDKLTPKTTFQWNEIRHVSFDDKKFTIRLVDAKVSNFIFYSQDLHINKMILDLCKGNHDLYMRRRKPDTMEIQQMKAQAKEEKQRRQIERKKFIREKKLREKAEHERYELEKSMEHLQNEMRMANDALRRSEETKELYFEKSRVNEEQMQLTECKANHFKTEMDRLRERQMKIEREKHDLEKKIRDADFYVHQLTVENDKREAETEKLRKELICAKMAEREATARLLEFLNSGRKSSTDSLLTASSVSHAANTASSMAAISTPSLITSSSTNDLETAGGAELTTHSSHYLVQGDNSSGISDDFEPKEFILTDNEMEQITNEMERNHLDYLRNSKQVQSQLQTLRSEIAPHKIEENQSNLDILSEAQIKAGENKYSTLKKLKSGSTKARVAFFEEL

Target ORF information:

RefSeq Version	NM_078689.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster merlin, transcript variant A (Mer), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_078689.4

ORF Insert Sequence:

ATGAGCCCCT TCGGCTCCAA GAAAAACCGA TCCCTGTCGG TGCGGGTGAG CACCTTCGAC 
TCGGAGCTGG AGTTCAAGCT GGAGCCGCGC GCCTCCGGCC AGGATCTGTT CGACCTGGTG 
TGCCGCACCA TCGGGCTGCG CGAGTCTTGG TACTTTGGGC TGCAGTACGT CGACACCCGC 
AGCAACGTGT CCTGGCTGAA AATGGAGAAG CGGGTGCGCG ACCAGCGCGT TGAGCTGCAC 
GCTAGCAATA ATGTGTATGT GTTTAGCTTC TACGCCAAGT TCTTTCCGGA GAACGTCAGC 
GAGGAGCTGA TCCAGGAGAT CACGCAGCAC CTGTTCTTCC TGCAGGTCAA GCAGAGCATC 
CTCTCCATGG ACATCTACTG CCGCCCGGAG GCGTCCGTGC TGCTGGCCTC CTACGCGGTG 
CACGTGCAAT ACGGTCCGTA CGACTACGAG ACCTACAAGG ACGGTATGCT GGCCGGGGGT 
GAGCTTCTGC CCAAGGGAGT GACCGATCAG TACCAGATGA CGCCCGAAAT GTGGGAGGAA 
CGGATCAAGA CATGGTACAT GGACCATGAG CCCATGACGC GCGACGAGGT GGAGATGGAG 
TACCTGAAGA TTGCTCAGGA CCTGGACATG TACGGAGTAA ACTACTTTCC TATTACGAAT 
AAGAACAAAA CCAAATTGTG GCTGGGCGTC ACCTCCGTGG GCCTAAACAT CTACGATGAG 
CGCGATAAGC TAACGCCAAA GACAACGTTC CAGTGGAACG AGATACGGCA CGTCAGCTTC 
GACGACAAGA AGTTCACCAT TCGCCTGGTG GATGCCAAGG TTTCGAATTT CATCTTTTAC 
TCGCAGGACC TCCACATCAA CAAAATGATT CTTGACCTGT GCAAGGGCAA TCACGATCTA 
TATATGCGCC GCCGCAAACC CGACACCATG GAAATCCAGC AGATGAAGGC CCAAGCCAAG 
GAGGAGAAGC AACGCCGCCA GATCGAACGC AAAAAGTTCA TCAGGGAAAA GAAGCTGCGC 
GAAAAGGCGG AGCACGAGCG CTACGAGCTG GAGAAGAGCA TGGAGCACCT GCAAAACGAA 
ATGCGCATGG CCAACGATGC ACTTCGGCGT TCCGAAGAGA CCAAGGAGCT GTACTTCGAA 
AAGAGTCGTG TCAACGAGGA GCAGATGCAG CTGACCGAGT GCAAGGCAAA CCACTTCAAG 
ACGGAAATGG ACCGGCTGCG CGAGCGACAA ATGAAAATCG AGCGGGAGAA GCACGACCTG 
GAGAAGAAGA TTCGCGACGC CGACTTCTAT GTCCACCAGC TGACGGTGGA GAACGACAAG 
CGGGAGGCCG AGACTGAGAA GCTGCGCAAG GAGCTGATCT GCGCCAAAAT GGCGGAGCGC 
GAGGCCACCG CTCGCCTGCT CGAATTCCTC AACAGCGGCC GCAAGTCCTC CACGGATAGC 
CTGCTGACCG CCTCCAGTGT GTCGCACGCG GCGAATACGG CGTCCAGCAT GGCGGCCATC 
AGCACACCCT CCCTGATCAC AAGCAGCTCA ACCAATGATT TGGAGACCGC CGGCGGCGCC 
GAGCTGACCA CCCACTCGTC ACACTACCTG GTCCAAGGTG ACAACTCCAG CGGCATTTCG 
GACGACTTCG AGCCCAAGGA GTTCATACTC ACCGACAACG AGATGGAGCA GATCACCAAT 
GAGATGGAGC GCAACCACCT CGACTACCTG CGCAACTCCA AGCAGGTGCA AAGTCAACTG 
CAGACACTGC GCTCCGAGAT CGCGCCGCAC AAGATTGAGG AGAACCAGAG CAACCTGGAC 
ATCCTCAGCG AGGCGCAGAT CAAGGCCGGC GAAAATAAAT ACTCCACGCT CAAGAAACTC 
AAGTCCGGCT CGACCAAGGC GCGTGTCGCC TTCTTCGAGG AGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mer ( NM_001298529.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001285458.1 Drosophila melanogaster merlin, transcript variant B (Mer), mRNA.

Price & Availability↑

CloneID	OFa02167
Clone ID Related Accession (Same CDS sequence)	NM_078689.4 , NM_001298529.1
Accession Version	NM_001298529.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1908bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	merlin, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAGCCCCT TCGGCTCCAA GAAAAACCGA TCCCTGTCGG TGCGGGTGAG CACCTTCGAC 
TCGGAGCTGG AGTTCAAGCT GGAGCCGCGC GCCTCCGGCC AGGATCTGTT CGACCTGGTG 
TGCCGCACCA TCGGGCTGCG CGAGTCTTGG TACTTTGGGC TGCAGTACGT CGACACCCGC 
AGCAACGTGT CCTGGCTGAA AATGGAGAAG CGGGTGCGCG ACCAGCGCGT TGAGCTGCAC 
GCTAGCAATA ATGTGTATGT GTTTAGCTTC TACGCCAAGT TCTTTCCGGA GAACGTCAGC 
GAGGAGCTGA TCCAGGAGAT CACGCAGCAC CTGTTCTTCC TGCAGGTCAA GCAGAGCATC 
CTCTCCATGG ACATCTACTG CCGCCCGGAG GCGTCCGTGC TGCTGGCCTC CTACGCGGTG 
CACGTGCAAT ACGGTCCGTA CGACTACGAG ACCTACAAGG ACGGTATGCT GGCCGGGGGT 
GAGCTTCTGC CCAAGGGAGT GACCGATCAG TACCAGATGA CGCCCGAAAT GTGGGAGGAA 
CGGATCAAGA CATGGTACAT GGACCATGAG CCCATGACGC GCGACGAGGT GGAGATGGAG 
TACCTGAAGA TTGCTCAGGA CCTGGACATG TACGGAGTAA ACTACTTTCC TATTACGAAT 
AAGAACAAAA CCAAATTGTG GCTGGGCGTC ACCTCCGTGG GCCTAAACAT CTACGATGAG 
CGCGATAAGC TAACGCCAAA GACAACGTTC CAGTGGAACG AGATACGGCA CGTCAGCTTC 
GACGACAAGA AGTTCACCAT TCGCCTGGTG GATGCCAAGG TTTCGAATTT CATCTTTTAC 
TCGCAGGACC TCCACATCAA CAAAATGATT CTTGACCTGT GCAAGGGCAA TCACGATCTA 
TATATGCGCC GCCGCAAACC CGACACCATG GAAATCCAGC AGATGAAGGC CCAAGCCAAG 
GAGGAGAAGC AACGCCGCCA GATCGAACGC AAAAAGTTCA TCAGGGAAAA GAAGCTGCGC 
GAAAAGGCGG AGCACGAGCG CTACGAGCTG GAGAAGAGCA TGGAGCACCT GCAAAACGAA 
ATGCGCATGG CCAACGATGC ACTTCGGCGT TCCGAAGAGA CCAAGGAGCT GTACTTCGAA 
AAGAGTCGTG TCAACGAGGA GCAGATGCAG CTGACCGAGT GCAAGGCAAA CCACTTCAAG 
ACGGAAATGG ACCGGCTGCG CGAGCGACAA ATGAAAATCG AGCGGGAGAA GCACGACCTG 
GAGAAGAAGA TTCGCGACGC CGACTTCTAT GTCCACCAGC TGACGGTGGA GAACGACAAG 
CGGGAGGCCG AGACTGAGAA GCTGCGCAAG GAGCTGATCT GCGCCAAAAT GGCGGAGCGC 
GAGGCCACCG CTCGCCTGCT CGAATTCCTC AACAGCGGCC GCAAGTCCTC CACGGATAGC 
CTGCTGACCG CCTCCAGTGT GTCGCACGCG GCGAATACGG CGTCCAGCAT GGCGGCCATC 
AGCACACCCT CCCTGATCAC AAGCAGCTCA ACCAATGATT TGGAGACCGC CGGCGGCGCC 
GAGCTGACCA CCCACTCGTC ACACTACCTG GTCCAAGGTG ACAACTCCAG CGGCATTTCG 
GACGACTTCG AGCCCAAGGA GTTCATACTC ACCGACAACG AGATGGAGCA GATCACCAAT 
GAGATGGAGC GCAACCACCT CGACTACCTG CGCAACTCCA AGCAGGTGCA AAGTCAACTG 
CAGACACTGC GCTCCGAGAT CGCGCCGCAC AAGATTGAGG AGAACCAGAG CAACCTGGAC 
ATCCTCAGCG AGGCGCAGAT CAAGGCCGGC GAAAATAAAT ACTCCACGCT CAAGAAACTC 
AAGTCCGGCT CGACCAAGGC GCGTGTCGCC TTCTTCGAGG AGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001285458.1
CDS	358..2265
Translation	MSPFGSKKNRSLSVRVSTFDSELEFKLEPRASGQDLFDLVCRTIGLRESWYFGLQYVDTRSNVSWLKMEKRVRDQRVELHASNNVYVFSFYAKFFPENVSEELIQEITQHLFFLQVKQSILSMDIYCRPEASVLLASYAVHVQYGPYDYETYKDGMLAGGELLPKGVTDQYQMTPEMWEERIKTWYMDHEPMTRDEVEMEYLKIAQDLDMYGVNYFPITNKNKTKLWLGVTSVGLNIYDERDKLTPKTTFQWNEIRHVSFDDKKFTIRLVDAKVSNFIFYSQDLHINKMILDLCKGNHDLYMRRRKPDTMEIQQMKAQAKEEKQRRQIERKKFIREKKLREKAEHERYELEKSMEHLQNEMRMANDALRRSEETKELYFEKSRVNEEQMQLTECKANHFKTEMDRLRERQMKIEREKHDLEKKIRDADFYVHQLTVENDKREAETEKLRKELICAKMAEREATARLLEFLNSGRKSSTDSLLTASSVSHAANTASSMAAISTPSLITSSSTNDLETAGGAELTTHSSHYLVQGDNSSGISDDFEPKEFILTDNEMEQITNEMERNHLDYLRNSKQVQSQLQTLRSEIAPHKIEENQSNLDILSEAQIKAGENKYSTLKKLKSGSTKARVAFFEEL

Target ORF information:

RefSeq Version	NM_001298529.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster merlin, transcript variant B (Mer), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001298529.1

ORF Insert Sequence:

ATGAGCCCCT TCGGCTCCAA GAAAAACCGA TCCCTGTCGG TGCGGGTGAG CACCTTCGAC 
TCGGAGCTGG AGTTCAAGCT GGAGCCGCGC GCCTCCGGCC AGGATCTGTT CGACCTGGTG 
TGCCGCACCA TCGGGCTGCG CGAGTCTTGG TACTTTGGGC TGCAGTACGT CGACACCCGC 
AGCAACGTGT CCTGGCTGAA AATGGAGAAG CGGGTGCGCG ACCAGCGCGT TGAGCTGCAC 
GCTAGCAATA ATGTGTATGT GTTTAGCTTC TACGCCAAGT TCTTTCCGGA GAACGTCAGC 
GAGGAGCTGA TCCAGGAGAT CACGCAGCAC CTGTTCTTCC TGCAGGTCAA GCAGAGCATC 
CTCTCCATGG ACATCTACTG CCGCCCGGAG GCGTCCGTGC TGCTGGCCTC CTACGCGGTG 
CACGTGCAAT ACGGTCCGTA CGACTACGAG ACCTACAAGG ACGGTATGCT GGCCGGGGGT 
GAGCTTCTGC CCAAGGGAGT GACCGATCAG TACCAGATGA CGCCCGAAAT GTGGGAGGAA 
CGGATCAAGA CATGGTACAT GGACCATGAG CCCATGACGC GCGACGAGGT GGAGATGGAG 
TACCTGAAGA TTGCTCAGGA CCTGGACATG TACGGAGTAA ACTACTTTCC TATTACGAAT 
AAGAACAAAA CCAAATTGTG GCTGGGCGTC ACCTCCGTGG GCCTAAACAT CTACGATGAG 
CGCGATAAGC TAACGCCAAA GACAACGTTC CAGTGGAACG AGATACGGCA CGTCAGCTTC 
GACGACAAGA AGTTCACCAT TCGCCTGGTG GATGCCAAGG TTTCGAATTT CATCTTTTAC 
TCGCAGGACC TCCACATCAA CAAAATGATT CTTGACCTGT GCAAGGGCAA TCACGATCTA 
TATATGCGCC GCCGCAAACC CGACACCATG GAAATCCAGC AGATGAAGGC CCAAGCCAAG 
GAGGAGAAGC AACGCCGCCA GATCGAACGC AAAAAGTTCA TCAGGGAAAA GAAGCTGCGC 
GAAAAGGCGG AGCACGAGCG CTACGAGCTG GAGAAGAGCA TGGAGCACCT GCAAAACGAA 
ATGCGCATGG CCAACGATGC ACTTCGGCGT TCCGAAGAGA CCAAGGAGCT GTACTTCGAA 
AAGAGTCGTG TCAACGAGGA GCAGATGCAG CTGACCGAGT GCAAGGCAAA CCACTTCAAG 
ACGGAAATGG ACCGGCTGCG CGAGCGACAA ATGAAAATCG AGCGGGAGAA GCACGACCTG 
GAGAAGAAGA TTCGCGACGC CGACTTCTAT GTCCACCAGC TGACGGTGGA GAACGACAAG 
CGGGAGGCCG AGACTGAGAA GCTGCGCAAG GAGCTGATCT GCGCCAAAAT GGCGGAGCGC 
GAGGCCACCG CTCGCCTGCT CGAATTCCTC AACAGCGGCC GCAAGTCCTC CACGGATAGC 
CTGCTGACCG CCTCCAGTGT GTCGCACGCG GCGAATACGG CGTCCAGCAT GGCGGCCATC 
AGCACACCCT CCCTGATCAC AAGCAGCTCA ACCAATGATT TGGAGACCGC CGGCGGCGCC 
GAGCTGACCA CCCACTCGTC ACACTACCTG GTCCAAGGTG ACAACTCCAG CGGCATTTCG 
GACGACTTCG AGCCCAAGGA GTTCATACTC ACCGACAACG AGATGGAGCA GATCACCAAT 
GAGATGGAGC GCAACCACCT CGACTACCTG CGCAACTCCA AGCAGGTGCA AAGTCAACTG 
CAGACACTGC GCTCCGAGAT CGCGCCGCAC AAGATTGAGG AGAACCAGAG CAACCTGGAC 
ATCCTCAGCG AGGCGCAGAT CAAGGCCGGC GAAAATAAAT ACTCCACGCT CAAGAAACTC 
AAGTCCGGCT CGACCAAGGC GCGTGTCGCC TTCTTCGAGG AGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Mer cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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