ORF » Species Summary » Danio rerio » nf2a cDNA ORF clone

nf2a cDNA ORF clone, Danio rerio(zebrafish)

The following nf2a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nf2a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa33063 NM_001128707.1
Latest version!		 Danio rerio neurofibromin 2a (merlin) (nf2a), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
ODa10153 XM_005165048.4
Latest version!		 Danio rerio neurofibromin 2a (merlin) (nf2a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
View Old Accession Versions >>
ODa10153 XM_005165048.3 Danio rerio neurofibromin 2a (merlin) (nf2a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    nf2a ( NM_001128707.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001122179.1 Danio rerio neurofibromin 2a (merlin) (nf2a), mRNA.
    Price & Availability↑
    CloneID ODa33063
    Clone ID Related Accession (Same CDS sequence) NM_001128707.1
    Accession Version NM_001128707.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1782bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-11-23
    Organism Danio rerio(zebrafish)
    Product merlin
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC162441.1. On Jul 31, 2008 this sequence version replaced XM_684590.1. ##Evidence-Data-START## Transcript exon combination :: BC162439.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGCTAGTG CCCTTGCATC AAAAATGGGA CTCTCTCTTC GGAGGAAGCA AGCCAAAATC 
    TTCAATGTTA CAGTTGTTAC AATGGATGCT GATTTGGAGT TCAGCTGTGA GCTGAAGTGG 
    CGAGGAAAGG ATCTGTTTGA ACTTGTATGC CGGACATTGG GGCTCAGGGA GACTTGGTTT 
    TTTGGTCTGC GGTACGACGT CAAAGACACT GTGGCCTGGC TGAAAATGGA CAAGAAGGTT 
    TTGGATCATG ATGTGCCCAA AGAGGAGCCT ATAGTGTTTT ATTTCCTGGC CAAGTTTTAC 
    CCTGAAAATG TAGAAGAAGA GTTGGTACAA GACATCACGC AGCACCTCTT CTTCCTCCAG 
    GTGAAGAAGA GCATTTTAGA AGAGGAGATC TACTGTCCTC CCGAGGCTTC GGTGCTGTTA 
    GCCTCTTACG CCGTCCATGC TAAGTATGGT GATTACAACC CTAATGTCCA CAAGCCGGGC 
    TTCCTGTCCC AAGACGAGCT GCTACCAAAG AGAGTTATTA ATCTGTACCA GATGACTGCA 
    GAGATGTGGG AGGAGAGAAT AACGGTCTGC TACGCTGAGC ACAGGGGCAG AACCAGAGAT 
    GAAGCAGAGA TGGAGTATTT AAAAATAGCT CAGGATCTGG AGATGTATGG GGTCAATTAC 
    TTTTCCATTA GGAATAAAAA GGGAACCAGC CTTCTGCTTG GTGTTGACGC TTTGGGCCTC 
    CACATTTATG ACCTGGATAA CAGACTGACG CCCAAAATCT CCTTTCCATG GAATGAGATC 
    CGAAACATCT CCTACAGCGA TAAAGAGTTT GCAATTAAAC CTGTGGATAA GAGAGCAGAT 
    GTCTTCAAAT TCAACTCTTC AAAGCTCAGA GTAAATAAAC TGATTCTCCA GCTGTGCATC 
    GGGAACCATG ATCTCTTCAT GAGGAGACGC AGGGTTGATT CATTGGAGGT CCAGCAGATG 
    AAGACTCAGG CTAGAGAAGA GAAAGCTCGC AAACAGATGG AGCGGCAGCG GCTAGAGAGA 
    GAGAAACAGT TGCGTGAGGA TGCAGAGAGA GCGCGTGATG AACTCCAGAG GAGACTCATT 
    CAGTTACAGG ACGAAGCTCA CTTGGCCAAT GAAGCACTGT TGCGTTCAGA GGAGACGGCA 
    GATTTGTTGG CGGAAAAGGC ACAAATCGCT GAGGAGGAGG CCAAGCTTTT GGCTCAGAAA 
    GCGGCTGAGG CTGAACAGGA GATGCAGCGT ATTAAGGTGA CAGCCATTCG CTCTGAGGAG 
    GAGAAGAGAC TGATGGAGCA GAAGATGCTG GAGGCTGAGA TGATTGCGCT CAAGATGGCA 
    GAAGAGTCTG AAAGGAGGGC CAAAGAGGCC GACCAGCTGA AACAGGACCT GCAGGAGGCC 
    AGAGAATCGG AGCGCAGAGC CAAAAACAAA CTACTGGAGA TCACCAGCAA AACGGAATAT 
    TCGCCTGTGC CGTTGTCTGC CAACTCTCTT CCTGCCGATG GTCCTAACTT CAACTTCAGC 
    ATGGAGAACC TGAGTTTTGA CTTCAAAGAC ACAGACATGA AACGTCTGTC CATGGAGATC 
    GAGAAGGAAA AGGTTGAGTA CATGGAGAAG AGCAAACACC TTCAGGAGCA GCTGAATGAA 
    CTGAAGACTG AGATTGAGTC ATTAAAGCTA AAGGAGCGAG AAACGCCTCT AGACATCCTC 
    CATAATGAGA ACACGGAGAA AGGCACCAGC AAGCAGAGCA ACTTTAAAAA GCTCACTCTA 
    CAGAGCACCA AATCCAGAGT GGCCTTCTTC CAGGAGCTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001122179.1
    CDS25..1806
    Translation

    Target ORF information:

    RefSeq Version NM_001128707.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio neurofibromin 2a (merlin) (nf2a), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001128707.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGCTAGTG CCCTTGCATC AAAAATGGGA CTCTCTCTTC GGAGGAAGCA AGCCAAAATC 
    TTCAATGTTA CAGTTGTTAC AATGGATGCT GATTTGGAGT TCAGCTGTGA GCTGAAGTGG 
    CGAGGAAAGG ATCTGTTTGA ACTTGTATGC CGGACATTGG GGCTCAGGGA GACTTGGTTT 
    TTTGGTCTGC GGTACGACGT CAAAGACACT GTGGCCTGGC TGAAAATGGA CAAGAAGGTT 
    TTGGATCATG ATGTGCCCAA AGAGGAGCCT ATAGTGTTTT ATTTCCTGGC CAAGTTTTAC 
    CCTGAAAATG TAGAAGAAGA GTTGGTACAA GACATCACGC AGCACCTCTT CTTCCTCCAG 
    GTGAAGAAGA GCATTTTAGA AGAGGAGATC TACTGTCCTC CCGAGGCTTC GGTGCTGTTA 
    GCCTCTTACG CCGTCCATGC TAAGTATGGT GATTACAACC CTAATGTCCA CAAGCCGGGC 
    TTCCTGTCCC AAGACGAGCT GCTACCAAAG AGAGTTATTA ATCTGTACCA GATGACTGCA 
    GAGATGTGGG AGGAGAGAAT AACGGTCTGC TACGCTGAGC ACAGGGGCAG AACCAGAGAT 
    GAAGCAGAGA TGGAGTATTT AAAAATAGCT CAGGATCTGG AGATGTATGG GGTCAATTAC 
    TTTTCCATTA GGAATAAAAA GGGAACCAGC CTTCTGCTTG GTGTTGACGC TTTGGGCCTC 
    CACATTTATG ACCTGGATAA CAGACTGACG CCCAAAATCT CCTTTCCATG GAATGAGATC 
    CGAAACATCT CCTACAGCGA TAAAGAGTTT GCAATTAAAC CTGTGGATAA GAGAGCAGAT 
    GTCTTCAAAT TCAACTCTTC AAAGCTCAGA GTAAATAAAC TGATTCTCCA GCTGTGCATC 
    GGGAACCATG ATCTCTTCAT GAGGAGACGC AGGGTTGATT CATTGGAGGT CCAGCAGATG 
    AAGACTCAGG CTAGAGAAGA GAAAGCTCGC AAACAGATGG AGCGGCAGCG GCTAGAGAGA 
    GAGAAACAGT TGCGTGAGGA TGCAGAGAGA GCGCGTGATG AACTCCAGAG GAGACTCATT 
    CAGTTACAGG ACGAAGCTCA CTTGGCCAAT GAAGCACTGT TGCGTTCAGA GGAGACGGCA 
    GATTTGTTGG CGGAAAAGGC ACAAATCGCT GAGGAGGAGG CCAAGCTTTT GGCTCAGAAA 
    GCGGCTGAGG CTGAACAGGA GATGCAGCGT ATTAAGGTGA CAGCCATTCG CTCTGAGGAG 
    GAGAAGAGAC TGATGGAGCA GAAGATGCTG GAGGCTGAGA TGATTGCGCT CAAGATGGCA 
    GAAGAGTCTG AAAGGAGGGC CAAAGAGGCC GACCAGCTGA AACAGGACCT GCAGGAGGCC 
    AGAGAATCGG AGCGCAGAGC CAAAAACAAA CTACTGGAGA TCACCAGCAA AACGGAATAT 
    TCGCCTGTGC CGTTGTCTGC CAACTCTCTT CCTGCCGATG GTCCTAACTT CAACTTCAGC 
    ATGGAGAACC TGAGTTTTGA CTTCAAAGAC ACAGACATGA AACGTCTGTC CATGGAGATC 
    GAGAAGGAAA AGGTTGAGTA CATGGAGAAG AGCAAACACC TTCAGGAGCA GCTGAATGAA 
    CTGAAGACTG AGATTGAGTC ATTAAAGCTA AAGGAGCGAG AAACGCCTCT AGACATCCTC 
    CATAATGAGA ACACGGAGAA AGGCACCAGC AAGCAGAGCA ACTTTAAAAA GCTCACTCTA 
    CAGAGCACCA AATCCAGAGT GGCCTTCTTC CAGGAGCTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    nf2a ( XM_005165048.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005165105.1 Danio rerio neurofibromin 2a (merlin) (nf2a), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID ODa10153
    Clone ID Related Accession (Same CDS sequence) XM_005165048.3 , XM_005165048.4
    Accession Version XM_005165048.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1782bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product merlin isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005165048.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGCTAGTG CCCTTGCATC AAAAATGGGA CTCTCTCTTC GGAGGAAGCA AGCCAAAATC 
    TTCAATGTTA CAGTTGTTAC AATGGATGCT GATTTGGAGT TCAGCTGTGA GCTGAAGTGG 
    CGAGGAAAGG ATCTGTTTGA ACTTGTATGC CGGACATTGG GGCTCAGGGA GACTTGGTTT 
    TTTGGTCTGC GGTACGACGT CAAAGACACT GTGGCCTGGC TGAAAATGGA CAAGAAGGTT 
    TTGGATCATG ATGTGCCCAA AGAGGAGCCT ATAGTGTTTT ATTTCCTGGC CAAGTTTTAC 
    CCTGAAAATG TAGAAGAAGA GTTGGTACAA GACATCACGC AGCACCTCTT CTTCCTCCAG 
    GTGAAGAAGA GCATTTTAGA AGAGGAGATC TACTGTCCTC CCGAGGCTTC GGTGCTGTTA 
    GCCTCTTACG CCGTCCATGC TAAGTATGGT GATTACAACC CTAATGTCCA CAAGCCGGGC 
    TTCCTGTCCC AAGACGAGCT GCTACCAAAG AGAGTTATTA ATCTGTACCA GATGACTGCA 
    GAGATGTGGG AGGAGAGAAT AACGGTCTGC TACGCTGAGC ACAGGGGCAG AACCAGAGAT 
    GAAGCAGAGA TGGAGTATTT AAAAATAGCT CAGGATCTGG AGATGTATGG GGTCAATTAC 
    TTTTCCATTA GGAATAAAAA GGGAACCAGC CTTCTGCTTG GTGTTGACGC TTTGGGCCTC 
    CACATTTATG ACCTGGATAA CAGACTGACG CCCAAAATCT CCTTTCCATG GAATGAGATC 
    CGAAACATCT CCTACAGCGA TAAAGAGTTT GCAATTAAAC CTGTGGATAA GAGAGCAGAT 
    GTCTTCAAAT TCAACTCTTC AAAGCTCAGA GTAAATAAAC TGATTCTCCA GCTGTGCATC 
    GGGAACCATG ATCTCTTCAT GAGGAGACGC AGGGTTGATT CATTGGAGGT CCAGCAGATG 
    AAGACTCAGG CTAGAGAAGA GAAAGCTCGC AAACAGATGG AGCGGCAGCG GCTAGAGAGA 
    GAGAAACAGT TGCGTGAGGA TGCAGAGAGA GCGCGTGATG AACTCCAGAG GAGACTCATT 
    CAGTTACAGG ACGAAGCTCA CTTGGCCAAT GAAGCACTGT TGCGTTCAGA GGAGACGGCA 
    GATTTGTTGG CGGAAAAGGC ACAAATCGCT GAGGAGGAGG CCAAGCTTTT GGCTCAGAAA 
    GCGGCTGAGG CTGAACAGGA GATGCAGCGT ATTAAGGTGA CAGCCATTCG CTCTGAGGAG 
    GAGAAGAGAC TGATGGAGCA GAAGATGCTG GAGGCTGAGA TGATTGCGCT CAAGATGGCA 
    GAAGAGTCTG AAAGGAGGGC CAAAGAGGCC GACCAGCTGA AACAGGACCT GCAGGAGGCC 
    AGAGAATCGG AGCGCAGAGC CAAAAACAAA CTACTGGAGA TCACCAGCAA AACGGAATAT 
    TCGCCTGTGC CGTTGTCTGC CAACTCTCTT CCTGCCGATG GTCCTAACTT CAACTTCAGC 
    ATGGAGAACC TGAGTTTTGA CTTCAAAGAC ACAGACATGA AACGTCTGTC CATGGAGATC 
    GAGAAGGAAA AGGTTGAGTA CATGGAGAAG AGCAAACACC TTCAGGAGCA ACTGAATGAA 
    CTGAAGACTG AGATTGAGTC ATTAAAGCTA AAGGAGCGAG AAACGCCTCT AGACATCCTC 
    CATAATGAGA ACACGGAGAA AGGCACCAGC AAGCAGAGCA ACTTTAAAAA GCTCACTCTA 
    CAGAGCACCA AATCCAGAGT GGCCTTCTTC CAGGAGCTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005165105.1
    CDS404..2185
    Translation

    Target ORF information:

    RefSeq Version XM_005165048.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio neurofibromin 2a (merlin) (nf2a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_005165048.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGCTAGTG CCCTTGCATC AAAAATGGGA CTCTCTCTTC GGAGGAAGCA AGCCAAAATC 
    TTCAATGTTA CAGTTGTTAC AATGGATGCT GATTTGGAGT TCAGCTGTGA GCTGAAGTGG 
    CGAGGAAAGG ATCTGTTTGA ACTTGTATGC CGGACATTGG GGCTCAGGGA GACTTGGTTT 
    TTTGGTCTGC GGTACGACGT CAAAGACACT GTGGCCTGGC TGAAAATGGA CAAGAAGGTT 
    TTGGATCATG ATGTGCCCAA AGAGGAGCCT ATAGTGTTTT ATTTCCTGGC CAAGTTTTAC 
    CCTGAAAATG TAGAAGAAGA GTTGGTACAA GACATCACGC AGCACCTCTT CTTCCTCCAG 
    GTGAAGAAGA GCATTTTAGA AGAGGAGATC TACTGTCCTC CCGAGGCTTC GGTGCTGTTA 
    GCCTCTTACG CCGTCCATGC TAAGTATGGT GATTACAACC CTAATGTCCA CAAGCCGGGC 
    TTCCTGTCCC AAGACGAGCT GCTACCAAAG AGAGTTATTA ATCTGTACCA GATGACTGCA 
    GAGATGTGGG AGGAGAGAAT AACGGTCTGC TACGCTGAGC ACAGGGGCAG AACCAGAGAT 
    GAAGCAGAGA TGGAGTATTT AAAAATAGCT CAGGATCTGG AGATGTATGG GGTCAATTAC 
    TTTTCCATTA GGAATAAAAA GGGAACCAGC CTTCTGCTTG GTGTTGACGC TTTGGGCCTC 
    CACATTTATG ACCTGGATAA CAGACTGACG CCCAAAATCT CCTTTCCATG GAATGAGATC 
    CGAAACATCT CCTACAGCGA TAAAGAGTTT GCAATTAAAC CTGTGGATAA GAGAGCAGAT 
    GTCTTCAAAT TCAACTCTTC AAAGCTCAGA GTAAATAAAC TGATTCTCCA GCTGTGCATC 
    GGGAACCATG ATCTCTTCAT GAGGAGACGC AGGGTTGATT CATTGGAGGT CCAGCAGATG 
    AAGACTCAGG CTAGAGAAGA GAAAGCTCGC AAACAGATGG AGCGGCAGCG GCTAGAGAGA 
    GAGAAACAGT TGCGTGAGGA TGCAGAGAGA GCGCGTGATG AACTCCAGAG GAGACTCATT 
    CAGTTACAGG ACGAAGCTCA CTTGGCCAAT GAAGCACTGT TGCGTTCAGA GGAGACGGCA 
    GATTTGTTGG CGGAAAAGGC ACAAATCGCT GAGGAGGAGG CCAAGCTTTT GGCTCAGAAA 
    GCGGCTGAGG CTGAACAGGA GATGCAGCGT ATTAAGGTGA CAGCCATTCG CTCTGAGGAG 
    GAGAAGAGAC TGATGGAGCA GAAGATGCTG GAGGCTGAGA TGATTGCGCT CAAGATGGCA 
    GAAGAGTCTG AAAGGAGGGC CAAAGAGGCC GACCAGCTGA AACAGGACCT GCAGGAGGCC 
    AGAGAATCGG AGCGCAGAGC CAAAAACAAA CTACTGGAGA TCACCAGCAA AACGGAATAT 
    TCGCCTGTGC CGTTGTCTGC CAACTCTCTT CCTGCCGATG GTCCTAACTT CAACTTCAGC 
    ATGGAGAACC TGAGTTTTGA CTTCAAAGAC ACAGACATGA AACGTCTGTC CATGGAGATC 
    GAGAAGGAAA AGGTTGAGTA CATGGAGAAG AGCAAACACC TTCAGGAGCA ACTGAATGAA 
    CTGAAGACTG AGATTGAGTC ATTAAAGCTA AAGGAGCGAG AAACGCCTCT AGACATCCTC 
    CATAATGAGA ACACGGAGAA AGGCACCAGC AAGCAGAGCA ACTTTAAAAA GCTCACTCTA 
    CAGAGCACCA AATCCAGAGT GGCCTTCTTC CAGGAGCTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    nf2a ( XM_005165048.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005165105.1 Danio rerio neurofibromin 2a (merlin) (nf2a), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID ODa10153
    Clone ID Related Accession (Same CDS sequence) XM_005165048.3 , XM_005165048.4
    Accession Version XM_005165048.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1782bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product merlin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005165048.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGCTAGTG CCCTTGCATC AAAAATGGGA CTCTCTCTTC GGAGGAAGCA AGCCAAAATC 
    TTCAATGTTA CAGTTGTTAC AATGGATGCT GATTTGGAGT TCAGCTGTGA GCTGAAGTGG 
    CGAGGAAAGG ATCTGTTTGA ACTTGTATGC CGGACATTGG GGCTCAGGGA GACTTGGTTT 
    TTTGGTCTGC GGTACGACGT CAAAGACACT GTGGCCTGGC TGAAAATGGA CAAGAAGGTT 
    TTGGATCATG ATGTGCCCAA AGAGGAGCCT ATAGTGTTTT ATTTCCTGGC CAAGTTTTAC 
    CCTGAAAATG TAGAAGAAGA GTTGGTACAA GACATCACGC AGCACCTCTT CTTCCTCCAG 
    GTGAAGAAGA GCATTTTAGA AGAGGAGATC TACTGTCCTC CCGAGGCTTC GGTGCTGTTA 
    GCCTCTTACG CCGTCCATGC TAAGTATGGT GATTACAACC CTAATGTCCA CAAGCCGGGC 
    TTCCTGTCCC AAGACGAGCT GCTACCAAAG AGAGTTATTA ATCTGTACCA GATGACTGCA 
    GAGATGTGGG AGGAGAGAAT AACGGTCTGC TACGCTGAGC ACAGGGGCAG AACCAGAGAT 
    GAAGCAGAGA TGGAGTATTT AAAAATAGCT CAGGATCTGG AGATGTATGG GGTCAATTAC 
    TTTTCCATTA GGAATAAAAA GGGAACCAGC CTTCTGCTTG GTGTTGACGC TTTGGGCCTC 
    CACATTTATG ACCTGGATAA CAGACTGACG CCCAAAATCT CCTTTCCATG GAATGAGATC 
    CGAAACATCT CCTACAGCGA TAAAGAGTTT GCAATTAAAC CTGTGGATAA GAGAGCAGAT 
    GTCTTCAAAT TCAACTCTTC AAAGCTCAGA GTAAATAAAC TGATTCTCCA GCTGTGCATC 
    GGGAACCATG ATCTCTTCAT GAGGAGACGC AGGGTTGATT CATTGGAGGT CCAGCAGATG 
    AAGACTCAGG CTAGAGAAGA GAAAGCTCGC AAACAGATGG AGCGGCAGCG GCTAGAGAGA 
    GAGAAACAGT TGCGTGAGGA TGCAGAGAGA GCGCGTGATG AACTCCAGAG GAGACTCATT 
    CAGTTACAGG ACGAAGCTCA CTTGGCCAAT GAAGCACTGT TGCGTTCAGA GGAGACGGCA 
    GATTTGTTGG CGGAAAAGGC ACAAATCGCT GAGGAGGAGG CCAAGCTTTT GGCTCAGAAA 
    GCGGCTGAGG CTGAACAGGA GATGCAGCGT ATTAAGGTGA CAGCCATTCG CTCTGAGGAG 
    GAGAAGAGAC TGATGGAGCA GAAGATGCTG GAGGCTGAGA TGATTGCGCT CAAGATGGCA 
    GAAGAGTCTG AAAGGAGGGC CAAAGAGGCC GACCAGCTGA AACAGGACCT GCAGGAGGCC 
    AGAGAATCGG AGCGCAGAGC CAAAAACAAA CTACTGGAGA TCACCAGCAA AACGGAATAT 
    TCGCCTGTGC CGTTGTCTGC CAACTCTCTT CCTGCCGATG GTCCTAACTT CAACTTCAGC 
    ATGGAGAACC TGAGTTTTGA CTTCAAAGAC ACAGACATGA AACGTCTGTC CATGGAGATC 
    GAGAAGGAAA AGGTTGAGTA CATGGAGAAG AGCAAACACC TTCAGGAGCA ACTGAATGAA 
    CTGAAGACTG AGATTGAGTC ATTAAAGCTA AAGGAGCGAG AAACGCCTCT AGACATCCTC 
    CATAATGAGA ACACGGAGAA AGGCACCAGC AAGCAGAGCA ACTTTAAAAA GCTCACTCTA 
    CAGAGCACCA AATCCAGAGT GGCCTTCTTC CAGGAGCTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005165105.1
    CDS398..2179
    Translation

    Target ORF information:

    RefSeq Version XM_005165048.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio neurofibromin 2a (merlin) (nf2a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_005165048.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGCTAGTG CCCTTGCATC AAAAATGGGA CTCTCTCTTC GGAGGAAGCA AGCCAAAATC 
    TTCAATGTTA CAGTTGTTAC AATGGATGCT GATTTGGAGT TCAGCTGTGA GCTGAAGTGG 
    CGAGGAAAGG ATCTGTTTGA ACTTGTATGC CGGACATTGG GGCTCAGGGA GACTTGGTTT 
    TTTGGTCTGC GGTACGACGT CAAAGACACT GTGGCCTGGC TGAAAATGGA CAAGAAGGTT 
    TTGGATCATG ATGTGCCCAA AGAGGAGCCT ATAGTGTTTT ATTTCCTGGC CAAGTTTTAC 
    CCTGAAAATG TAGAAGAAGA GTTGGTACAA GACATCACGC AGCACCTCTT CTTCCTCCAG 
    GTGAAGAAGA GCATTTTAGA AGAGGAGATC TACTGTCCTC CCGAGGCTTC GGTGCTGTTA 
    GCCTCTTACG CCGTCCATGC TAAGTATGGT GATTACAACC CTAATGTCCA CAAGCCGGGC 
    TTCCTGTCCC AAGACGAGCT GCTACCAAAG AGAGTTATTA ATCTGTACCA GATGACTGCA 
    GAGATGTGGG AGGAGAGAAT AACGGTCTGC TACGCTGAGC ACAGGGGCAG AACCAGAGAT 
    GAAGCAGAGA TGGAGTATTT AAAAATAGCT CAGGATCTGG AGATGTATGG GGTCAATTAC 
    TTTTCCATTA GGAATAAAAA GGGAACCAGC CTTCTGCTTG GTGTTGACGC TTTGGGCCTC 
    CACATTTATG ACCTGGATAA CAGACTGACG CCCAAAATCT CCTTTCCATG GAATGAGATC 
    CGAAACATCT CCTACAGCGA TAAAGAGTTT GCAATTAAAC CTGTGGATAA GAGAGCAGAT 
    GTCTTCAAAT TCAACTCTTC AAAGCTCAGA GTAAATAAAC TGATTCTCCA GCTGTGCATC 
    GGGAACCATG ATCTCTTCAT GAGGAGACGC AGGGTTGATT CATTGGAGGT CCAGCAGATG 
    AAGACTCAGG CTAGAGAAGA GAAAGCTCGC AAACAGATGG AGCGGCAGCG GCTAGAGAGA 
    GAGAAACAGT TGCGTGAGGA TGCAGAGAGA GCGCGTGATG AACTCCAGAG GAGACTCATT 
    CAGTTACAGG ACGAAGCTCA CTTGGCCAAT GAAGCACTGT TGCGTTCAGA GGAGACGGCA 
    GATTTGTTGG CGGAAAAGGC ACAAATCGCT GAGGAGGAGG CCAAGCTTTT GGCTCAGAAA 
    GCGGCTGAGG CTGAACAGGA GATGCAGCGT ATTAAGGTGA CAGCCATTCG CTCTGAGGAG 
    GAGAAGAGAC TGATGGAGCA GAAGATGCTG GAGGCTGAGA TGATTGCGCT CAAGATGGCA 
    GAAGAGTCTG AAAGGAGGGC CAAAGAGGCC GACCAGCTGA AACAGGACCT GCAGGAGGCC 
    AGAGAATCGG AGCGCAGAGC CAAAAACAAA CTACTGGAGA TCACCAGCAA AACGGAATAT 
    TCGCCTGTGC CGTTGTCTGC CAACTCTCTT CCTGCCGATG GTCCTAACTT CAACTTCAGC 
    ATGGAGAACC TGAGTTTTGA CTTCAAAGAC ACAGACATGA AACGTCTGTC CATGGAGATC 
    GAGAAGGAAA AGGTTGAGTA CATGGAGAAG AGCAAACACC TTCAGGAGCA ACTGAATGAA 
    CTGAAGACTG AGATTGAGTC ATTAAAGCTA AAGGAGCGAG AAACGCCTCT AGACATCCTC 
    CATAATGAGA ACACGGAGAA AGGCACCAGC AAGCAGAGCA ACTTTAAAAA GCTCACTCTA 
    CAGAGCACCA AATCCAGAGT GGCCTTCTTC CAGGAGCTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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