Hmgcr cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hmgcr
Entrez Gene ID	25675
Full Name	3-hydroxy-3-methylglutaryl-CoA reductase
Synonyms	3H3M
General protein information	Preferred Names 3-hydroxy-3-methylglutaryl-CoA reductase Names 3-hydroxy-3-methylglutaryl-coenzyme A reductase HMG-CoA reductase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q12
Summary	enzyme involved in mevalonate synthesis [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006231826.3	XP_006231888.1	3-hydroxy-3-methylglutaryl-coenzyme A reductase isoform X1
NM_013134.2	NP_037266.2	3-hydroxy-3-methylglutaryl-coenzyme A reductase

Pathways from BioSystems

Homologies

Homo sapiens (human)	HMGCR	NP_000850.1
Gallus gallus (chicken)	HMGCR	NP_989816.1
Caenorhabditis elegans (roundworm)	hmgr-1	NP_498626.2
Saccharomyces cerevisiae (baker's yeast)	HMG2	NP_013555.1
Kluyveromyces lactis	KLLA0B04642g	XP_451740.1
Pan troglodytes (chimpanzee)	HMGCR	XP_001148405.2
Bos taurus (cattle)	HMGCR	NP_001099083.1
Xenopus tropicalis (tropical clawed frog)	hmgcr	XP_004910453.1
	HMGCR	XP_002804463.1
Canis lupus familiaris (dog)	HMGCR	XP_536323.3
Drosophila melanogaster (fruit fly)	Hmgcr	NP_732900.1
Magnaporthe oryzae (rice blast fungus)	MGG_08975	XP_003714014.1
Neurospora crassa	NCU00712	XP_964546.1
Arabidopsis thaliana (thale cress)	HMG1	NP_177775.2
Oryza sativa (rice)	Os09g0492700	NP_001063541.1
Oryza sativa (rice)	Os08g0512700	NP_001062221.1
Mus musculus (house mouse)	Hmgcr	NP_032281.2
Rattus norvegicus (Norway rat)	Hmgcr	NP_037266.2
Danio rerio (zebrafish)	hmgcra	NP_001073446.1
Arabidopsis thaliana (thale cress)	HMG2	NP_179329.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002288	XP_307890.5
Saccharomyces cerevisiae (baker's yeast)	HMG1	NP_013636.1
Eremothecium gossypii	AGOS_AER152W	NP_985010.2
Schizosaccharomyces pombe (fission yeast)	hmg1	NP_588235.1

Gene Ontology
Bibliography

Related articles in PubMed

Regulation of hepatic hydroxy methyl glutarate - CoA reductase for controlling hypercholesterolemia in rats.
Khamis AA, Salama AF, Kenawy ME, Mohamed TM
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie951242-1250(2017 Nov)

Adverse effects of long-term exposure to bisphenol A during adulthood leading to hyperglycaemia and hypercholesterolemia in mice.
Marmugi A, Lasserre F, Beuzelin D, Ducheix S, Huc L, Polizzi A, Chetivaux M, Pineau T, Martin P, Guillou H, Mselli-Lakhal L
Toxicology325133-43(2014 Nov)

Involvement of hepatic IL-1 in the strain-dependent sex differences in serum total cholesterol levels in rats.
Kumagai Y, Sekimoto M, Okamoto M, Kurita R, Kojima M, Degawa M
Biological & pharmaceutical bulletin37(4)654-8(2014)

Effects of simvaglyzin and atorvaglyzin on the expression of 3-hydroxy-3-methyl-glutaryl-CoA reductase in rat liver.
Stakhneva EM, Vavilin VA, Ragino YI, Safronova OG, Shintyapina AB, Ivanova MV
Bulletin of experimental biology and medicine156(1)63-5(2013 Nov)

Hypocholesterolemic effect of capsaicinoids in rats fed diets with or without cholesterol.
Zhang L, Fang G, Zheng L, Chen Z, Liu X
Journal of agricultural and food chemistry61(18)4287-93(2013 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

findings demonstrate for the first time that strain-dependent sex difference in serum Total Cholesterol level between F344 and SD rats is, at least in part, related to difference in the IL-1alpha-mediated HMGR gene expression level in the liver.
Title: Involvement of hepatic IL-1 in the strain-dependent sex differences in serum total cholesterol levels in rats.

HMGCR expression is reduced in the liver of rats exposed to simvaglyzin and atorvaglyzin.
Title: Effects of simvaglyzin and atorvaglyzin on the expression of 3-hydroxy-3-methyl-glutaryl-CoA reductase in rat liver.

investigated 3-hydroxy 3-methylglutaryl coenzyme A reductase and LDLr in adult (3-month-old) and aged (12-month-old) male and female rats in four different brain regions
Title: Analysis of the protein network of cholesterol homeostasis in different brain regions: an age and sex dependent perspective.

Capsaicinoids consumption down-regulated the mRNA levels of HMG-CoA reductase and CYP7A1 in the cholesterol-free diet group but up-regulated the CYP7A1 level in the cholesterol-enriched diet group.
Title: Hypocholesterolemic effect of capsaicinoids in rats fed diets with or without cholesterol.

These findings indicate a role for TTP in the insulin-mediated activation of hepatic HMGR transcription.
Title: Involvement of tristetraprolin in transcriptional activation of hepatic 3-hydroxy-3-methylglutaryl coenzyme A reductase by insulin.

The following Hmgcr gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hmgcr cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa02098	NM_013134.2 Latest version!	Rattus norvegicus 3-hydroxy-3-methylglutaryl-CoA reductase (Hmgcr), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$524.30 $749.00
ORa24340	XM_006231826.3 Latest version!	Rattus norvegicus 3-hydroxy-3-methylglutaryl-CoA reductase (Hmgcr), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hmgcr ( NM_013134.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_037266.2 Rattus norvegicus 3-hydroxy-3-methylglutaryl-CoA reductase (Hmgcr), mRNA.

Price & Availability↑

CloneID	ORa02098
Clone ID Related Accession (Same CDS sequence)	NM_013134.2
Accession Version	NM_013134.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2664bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Rattus norvegicus(Norway rat)
Product	3-hydroxy-3-methylglutaryl-coenzyme A reductase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC064654.1. On Jan 1, 2004 this sequence version replaced NM_013134.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC064654.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGTTGTCAA GACTTTTCCG TATGCATGGC CTCTTTGTGG CCTCCCATCC CTGGGAGGTA 
ATTGTGGGAA CGGTGACACT TACTATCTGT ATGATGTCCA TGAACATGTT CACCGGCAAC 
AACAAGATCT GTGGTTGGAA TTATGAGTGC CCAAAATTTG AAGAGGACGT GCTGAGCAGC 
GACATCATCA TCCTCACGAT AACCCGGTGC ATCGCCATCC TGTACATCTA CTTCCAGTTC 
CAGAACCTGC GTCAGCTTGG GTCAAAGTAC ATTTTGGGTA TTGCCGGCCT CTTCACAATT 
TTCTCAAGTT TCGTCTTCAG CACTGTCGTC ATTCATTTCC TCGACAAAGA ATTGACAGGC 
TTAAATGAAG CTTTGCCCTT TTTTCTGCTC TTGATTGACC TTTCTAGAGC GAGTGCATTG 
GCCAAGTTTG CCCTGAGTTC AAACTCACAG GATGAAGTAA GGGAGAATAT AGCGCGTGGG 
ATGGCGATCC TGGGCCCCAC GTTCACCCTT GACGCTCTGG TGGAATGTCT TGTGATTGGA 
GTTGGCACCA TGTCAGGGGT GCGGCAGCTT GAGATCATGT GCTGCTTTGG CTGTATGTCC 
GTGCTTGCCA ACTACTTTGT CTTCATGACA TTCTTCCCAG CCTGCGTGTC CCTGGTCCTA 
GAGCTTTCTC GGGAAAGCCG TGAGGGTCGT CCAATTTGGC AGCTCAGCCA TTTTGCCAGA 
GTTTTAGAAG AAGAAGAGAA TAAACCAAAC CCAGTAACCC AAAGGGTCAA GATGATCATG 
TCTTTAGGCC TGGTTCTTGT TCACGCTCAC AGTCGCTGGA TAGCTGATCC TTCTCCTCAG 
AACAGCACAG CAGAACAGTC TAAGGTTTCC TTGGGTCTGG CTGAAGATGT GTCCAAGAGA 
ATTGAGCCGA GTGTTTCTCT CTGGCAGTTT TACCTCTCCA AGATGATCAG CATGGACATC 
GAGCAAGTGA TTACCCTGAG CTTAGCGTTG CTTTTGGCTG TCAAGTATAT TTTCTTTGAA 
CAAGCAGAGA CAGAATCAAC ACTCTCATTA AAAAATCCTA TCACATCTCC TGTCGTGACC 
CCAAAGAAAG CTCAAGACAA CTGTTGTAGA CGTGAGCCTC TGCTTGTGAG GAGGAACCAG 
AAGCTTTCGT CAGTGGAGGA GGATCCAGGA GTGAACCAAG ACAGAAAAGT TGAGGTTATA 
AAACCTTTAG TGGCAGAAGC CGAGACTTCG GGCAGAGCTA CGTTTGTGCT TGGCGCCTCT 
GCAGCCAGCC CTCCATTGGC CCTGGGGGCA CAGGAGCCTG GGATCGAACT CCCCAGCGAG 
CCTCGACCTA ATGAAGAGTG TCTACAGATA CTGGAGAGTG CAGAGAAAGG TGCGAAGTTC 
CTTAGTGATG CAGAGATCAT CCAGTTGGTC AATGCTAAGC ACATCCCAGC CTACAAACTG 
GAAACCCTCA TGGAGACGCA CGAGCGTGGT GTGTCTATTC GCCGGCAGCT CCTCTCCGCC 
AAGCTTGCAG AGCCATCTTC TCTGCAGTAC CTGCCTTACA GAGACTATAA TTACTCCTTG 
GTGATGGGAG CTTGCTGTGA GAACGTGATC GGATATATGC CCATCCCTGT TGGAGTGGCA 
GGACCTCTGT GCCTGGATGG AAAAGAGTAC CAGGTGCCAA TGGCAACAAC AGAAGGTTGT 
CTTGTGGCCA GCACGAACAG AGGCTGCAGA GCGATCAGTC TTGGTGGAGG TGCCAGCAGC 
CGGGTCCTTG CAGATGGGAT GAGCCGAGGC CCAGTGGTGC GTCTTCCTCG TGCTTGTGAC 
TCAGCAGAGG TGAAGAGCTG GCTTGAAACA CCTGAAGGGT TTGCAGTGGT AAAGGAGGCC 
TTCGACAGCA CGAGCAGATT TGCACGTCTA CAGAAACTTC ACGTGACGCT GGCAGGACGC 
AACCTCTACA TCCGTCTCCA GTCCAAAACG GGGGACGCCA TGGGGATGAA CATGATTTCC 
AAGGGTACGG AGAAAGCACT TCTGAAGCTT CAAGAGTTCT TTCCTGAGCT GCAGATTCTG 
GCGGTCAGTG GTAACTATTG CACCGACAAG AAACCTGCTG CCATAAACTG GATCGAAGGG 
AGAGGAAAGA CTGTGGTTTG TGAAGCTGTC ATTCCAGCCA AGGTGGTGAG AGAAGTATTA 
AAGACGACTA CGGAAGCTAT GGTTGACGTA AACATTAACA AGAATCTTGT GGGCTCTGCC 
ATGGCTGGTA GCATAGGAGG CTACAACGCC CATGCTGCCA ACATCGTCAC TGCCATCTAC 
ATTGCATGTG GCCAGGATGC AGCACAGAAT GTGGGGAGTT CAAACTGTAT TACGTTAATG 
GAAGCAAGTG GTCCCACAAA TGAAGACTTA TACATCAGCT GTACCATGCC GTCTATAGAG 
ATCGGAACCG TGGGTGGTGG GACCAACCTT CTACCTCAGC AAGCCTGCCT GCAGATGCTA 
GGTGTTCAAG GGGCGTGCAA AGACAATCCT GGAGAAAATG CACGGCAGCT TGCCCGAATT 
GTGTGTGGCA CTGTGATGGC TGGTGAGTTG TCCTTGATGG CAGCATTGGC AGCAGGACAT 
CTTGTCAGAA GTCACATGGT TCACAACAGA TCAAAGATAA ATTTACAAGA TCTGCAGGGA 
ACATGCACCA AGAAGGCAGC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037266.2
CDS	51..2714
Translation	MLSRLFRMHGLFVASHPWEVIVGTVTLTICMMSMNMFTGNNKICGWNYECPKFEEDVLSSDIIILTITRCIAILYIYFQFQNLRQLGSKYILGIAGLFTIFSSFVFSTVVIHFLDKELTGLNEALPFFLLLIDLSRASALAKFALSSNSQDEVRENIARGMAILGPTFTLDALVECLVIGVGTMSGVRQLEIMCCFGCMSVLANYFVFMTFFPACVSLVLELSRESREGRPIWQLSHFARVLEEEENKPNPVTQRVKMIMSLGLVLVHAHSRWIADPSPQNSTAEQSKVSLGLAEDVSKRIEPSVSLWQFYLSKMISMDIEQVITLSLALLLAVKYIFFEQAETESTLSLKNPITSPVVTPKKAQDNCCRREPLLVRRNQKLSSVEEDPGVNQDRKVEVIKPLVAEAETSGRATFVLGASAASPPLALGAQEPGIELPSEPRPNEECLQILESAEKGAKFLSDAEIIQLVNAKHIPAYKLETLMETHERGVSIRRQLLSAKLAEPSSLQYLPYRDYNYSLVMGACCENVIGYMPIPVGVAGPLCLDGKEYQVPMATTEGCLVASTNRGCRAISLGGGASSRVLADGMSRGPVVRLPRACDSAEVKSWLETPEGFAVVKEAFDSTSRFARLQKLHVTLAGRNLYIRLQSKTGDAMGMNMISKGTEKALLKLQEFFPELQILAVSGNYCTDKKPAAINWIEGRGKTVVCEAVIPAKVVREVLKTTTEAMVDVNINKNLVGSAMAGSIGGYNAHAANIVTAIYIACGQDAAQNVGSSNCITLMEASGPTNEDLYISCTMPSIEIGTVGGGTNLLPQQACLQMLGVQGACKDNPGENARQLARIVCGTVMAGELSLMAALAAGHLVRSHMVHNRSKINLQDLQGTCTKKAA

Target ORF information:

RefSeq Version	NM_013134.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus 3-hydroxy-3-methylglutaryl-CoA reductase (Hmgcr), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013134.2

ORF Insert Sequence:

ATGTTGTCAA GACTTTTCCG TATGCATGGC CTCTTTGTGG CCTCCCATCC CTGGGAGGTA 
ATTGTGGGAA CGGTGACACT TACTATCTGT ATGATGTCCA TGAACATGTT CACCGGCAAC 
AACAAGATCT GTGGTTGGAA TTATGAGTGC CCAAAATTTG AAGAGGACGT GCTGAGCAGC 
GACATCATCA TCCTCACGAT AACCCGGTGC ATCGCCATCC TGTACATCTA CTTCCAGTTC 
CAGAACCTGC GTCAGCTTGG GTCAAAGTAC ATTTTGGGTA TTGCCGGCCT CTTCACAATT 
TTCTCAAGTT TCGTCTTCAG CACTGTCGTC ATTCATTTCC TCGACAAAGA ATTGACAGGC 
TTAAATGAAG CTTTGCCCTT TTTTCTGCTC TTGATTGACC TTTCTAGAGC GAGTGCATTG 
GCCAAGTTTG CCCTGAGTTC AAACTCACAG GATGAAGTAA GGGAGAATAT AGCGCGTGGG 
ATGGCGATCC TGGGCCCCAC GTTCACCCTT GACGCTCTGG TGGAATGTCT TGTGATTGGA 
GTTGGCACCA TGTCAGGGGT GCGGCAGCTT GAGATCATGT GCTGCTTTGG CTGTATGTCC 
GTGCTTGCCA ACTACTTTGT CTTCATGACA TTCTTCCCAG CCTGCGTGTC CCTGGTCCTA 
GAGCTTTCTC GGGAAAGCCG TGAGGGTCGT CCAATTTGGC AGCTCAGCCA TTTTGCCAGA 
GTTTTAGAAG AAGAAGAGAA TAAACCAAAC CCAGTAACCC AAAGGGTCAA GATGATCATG 
TCTTTAGGCC TGGTTCTTGT TCACGCTCAC AGTCGCTGGA TAGCTGATCC TTCTCCTCAG 
AACAGCACAG CAGAACAGTC TAAGGTTTCC TTGGGTCTGG CTGAAGATGT GTCCAAGAGA 
ATTGAGCCGA GTGTTTCTCT CTGGCAGTTT TACCTCTCCA AGATGATCAG CATGGACATC 
GAGCAAGTGA TTACCCTGAG CTTAGCGTTG CTTTTGGCTG TCAAGTATAT TTTCTTTGAA 
CAAGCAGAGA CAGAATCAAC ACTCTCATTA AAAAATCCTA TCACATCTCC TGTCGTGACC 
CCAAAGAAAG CTCAAGACAA CTGTTGTAGA CGTGAGCCTC TGCTTGTGAG GAGGAACCAG 
AAGCTTTCGT CAGTGGAGGA GGATCCAGGA GTGAACCAAG ACAGAAAAGT TGAGGTTATA 
AAACCTTTAG TGGCAGAAGC CGAGACTTCG GGCAGAGCTA CGTTTGTGCT TGGCGCCTCT 
GCAGCCAGCC CTCCATTGGC CCTGGGGGCA CAGGAGCCTG GGATCGAACT CCCCAGCGAG 
CCTCGACCTA ATGAAGAGTG TCTACAGATA CTGGAGAGTG CAGAGAAAGG TGCGAAGTTC 
CTTAGTGATG CAGAGATCAT CCAGTTGGTC AATGCTAAGC ACATCCCAGC CTACAAACTG 
GAAACCCTCA TGGAGACGCA CGAGCGTGGT GTGTCTATTC GCCGGCAGCT CCTCTCCGCC 
AAGCTTGCAG AGCCATCTTC TCTGCAGTAC CTGCCTTACA GAGACTATAA TTACTCCTTG 
GTGATGGGAG CTTGCTGTGA GAACGTGATC GGATATATGC CCATCCCTGT TGGAGTGGCA 
GGACCTCTGT GCCTGGATGG AAAAGAGTAC CAGGTGCCAA TGGCAACAAC AGAAGGTTGT 
CTTGTGGCCA GCACGAACAG AGGCTGCAGA GCGATCAGTC TTGGTGGAGG TGCCAGCAGC 
CGGGTCCTTG CAGATGGGAT GAGCCGAGGC CCAGTGGTGC GTCTTCCTCG TGCTTGTGAC 
TCAGCAGAGG TGAAGAGCTG GCTTGAAACA CCTGAAGGGT TTGCAGTGGT AAAGGAGGCC 
TTCGACAGCA CGAGCAGATT TGCACGTCTA CAGAAACTTC ACGTGACGCT GGCAGGACGC 
AACCTCTACA TCCGTCTCCA GTCCAAAACG GGGGACGCCA TGGGGATGAA CATGATTTCC 
AAGGGTACGG AGAAAGCACT TCTGAAGCTT CAAGAGTTCT TTCCTGAGCT GCAGATTCTG 
GCGGTCAGTG GTAACTATTG CACCGACAAG AAACCTGCTG CCATAAACTG GATCGAAGGG 
AGAGGAAAGA CTGTGGTTTG TGAAGCTGTC ATTCCAGCCA AGGTGGTGAG AGAAGTATTA 
AAGACGACTA CGGAAGCTAT GGTTGACGTA AACATTAACA AGAATCTTGT GGGCTCTGCC 
ATGGCTGGTA GCATAGGAGG CTACAACGCC CATGCTGCCA ACATCGTCAC TGCCATCTAC 
ATTGCATGTG GCCAGGATGC AGCACAGAAT GTGGGGAGTT CAAACTGTAT TACGTTAATG 
GAAGCAAGTG GTCCCACAAA TGAAGACTTA TACATCAGCT GTACCATGCC GTCTATAGAG 
ATCGGAACCG TGGGTGGTGG GACCAACCTT CTACCTCAGC AAGCCTGCCT GCAGATGCTA 
GGTGTTCAAG GGGCGTGCAA AGACAATCCT GGAGAAAATG CACGGCAGCT TGCCCGAATT 
GTGTGTGGCA CTGTGATGGC TGGTGAGTTG TCCTTGATGG CAGCATTGGC AGCAGGACAT 
CTTGTCAGAA GTCACATGGT TCACAACAGA TCAAAGATAA ATTTACAAGA TCTGCAGGGA 
ACATGCACCA AGAAGGCAGC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Hmgcr ( XM_006231826.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006231888.1 Rattus norvegicus 3-hydroxy-3-methylglutaryl-CoA reductase (Hmgcr), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa24340
Clone ID Related Accession (Same CDS sequence)	XM_006231826.3
Accession Version	XM_006231826.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2694bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	3-hydroxy-3-methylglutaryl-coenzyme A reductase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006231826.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGAGGAT CCAAGGACTG TGTAGCTACA ATGTTGTCAA GACTTTTCCG TATGCATGGC 
CTCTTTGTGG CCTCCCATCC CTGGGAGGTA ATTGTGGGAA CGGTGACACT TACTATCTGT 
ATGATGTCCA TGAACATGTT CACCGGCAAC AACAAGATCT GTGGTTGGAA TTATGAGTGC 
CCAAAATTTG AAGAGGACGT GCTGAGCAGC GACATCATCA TCCTCACGAT AACCCGGTGC 
ATCGCCATCC TGTACATCTA CTTCCAGTTC CAGAACCTGC GTCAGCTTGG GTCAAAGTAC 
ATTTTGGGTA TTGCCGGCCT CTTCACAATT TTCTCAAGTT TCGTCTTCAG CACTGTCGTC 
ATTCATTTCC TCGACAAAGA ATTGACAGGC TTAAATGAAG CTTTGCCCTT TTTTCTGCTC 
TTGATTGACC TTTCTAGAGC GAGTGCATTG GCCAAGTTTG CCCTGAGTTC AAACTCACAG 
GATGAAGTAA GGGAGAATAT AGCGCGTGGG ATGGCGATCC TGGGCCCCAC GTTCACCCTT 
GACGCTCTGG TGGAATGTCT TGTGATTGGA GTTGGCACCA TGTCAGGGGT GCGGCAGCTT 
GAGATCATGT GCTGCTTTGG CTGTATGTCC GTGCTTGCCA ACTACTTTGT CTTCATGACA 
TTCTTCCCAG CCTGCGTGTC CCTGGTCCTA GAGCTTTCTC GGGAAAGCCG TGAGGGTCGT 
CCAATTTGGC AGCTCAGCCA TTTTGCCAGA GTTTTAGAAG AAGAAGAGAA TAAACCAAAC 
CCAGTAACCC AAAGGGTCAA GATGATCATG TCTTTAGGCC TGGTTCTTGT TCACGCTCAC 
AGTCGCTGGA TAGCTGATCC TTCTCCTCAG AACAGCACAG CAGAACAGTC TAAGGTTTCC 
TTGGGTCTGG CTGAAGATGT GTCCAAGAGA ATTGAGCCGA GTGTTTCTCT CTGGCAGTTT 
TACCTCTCCA AGATGATCAG CATGGACATC GAGCAAGTGA TTACCCTGAG CTTAGCGTTG 
CTTTTGGCTG TCAAGTATAT TTTCTTTGAA CAAGCAGAGA CAGAATCAAC ACTCTCATTA 
AAAAATCCTA TCACATCTCC TGTCGTGACC CCAAAGAAAG CTCAAGACAA CTGTTGTAGA 
CGTGAGCCTC TGCTTGTGAG GAGGAACCAG AAGCTTTCGT CAGTGGAGGA GGATCCAGGA 
GTGAACCAAG ACAGAAAAGT TGAGGTTATA AAACCTTTAG TGGCAGAAGC CGAGACTTCG 
GGCAGAGCTA CGTTTGTGCT TGGCGCCTCT GCAGCCAGCC CTCCATTGGC CCTGGGGGCA 
CAGGAGCCTG GGATCGAACT CCCCAGCGAG CCTCGACCTA ATGAAGAGTG TCTACAGATA 
CTGGAGAGTG CAGAGAAAGG TGCGAAGTTC CTTAGTGATG CAGAGATCAT CCAGTTGGTC 
AATGCTAAGC ACATCCCAGC CTACAAACTG GAAACCCTCA TGGAGACGCA CGAGCGTGGT 
GTGTCTATTC GCCGGCAGCT CCTCTCCGCC AAGCTTGCAG AGCCATCTTC TCTGCAGTAC 
CTGCCTTACA GAGACTATAA TTACTCCTTG GTGATGGGAG CTTGCTGTGA GAACGTGATC 
GGATATATGC CCATCCCTGT TGGAGTGGCA GGACCTCTGT GCCTGGATGG AAAAGAGTAC 
CAGGTGCCAA TGGCAACAAC AGAAGGTTGT CTTGTGGCCA GCACGAACAG AGGCTGCAGA 
GCGATCAGTC TTGGTGGAGG TGCCAGCAGC CGGGTCCTTG CAGATGGGAT GAGCCGAGGC 
CCAGTGGTGC GTCTTCCTCG TGCTTGTGAC TCAGCAGAGG TGAAGAGCTG GCTTGAAACA 
CCTGAAGGGT TTGCAGTGGT AAAGGAGGCC TTCGACAGCA CGAGCAGATT TGCACGTCTA 
CAGAAACTTC ACGTGACGCT GGCAGGACGC AACCTCTACA TCCGTCTCCA GTCCAAAACG 
GGGGACGCCA TGGGGATGAA CATGATTTCC AAGGGTACGG AGAAAGCACT TCTGAAGCTT 
CAAGAGTTCT TTCCTGAGCT GCAGATTCTG GCGGTCAGTG GTAACTATTG CACCGACAAG 
AAACCTGCTG CCATAAACTG GATCGAAGGG AGAGGAAAGA CTGTGGTTTG TGAAGCTGTC 
ATTCCAGCCA AGGTGGTGAG AGAAGTATTA AAGACGACTA CGGAAGCTAT GGTTGACGTA 
AACATTAACA AGAATCTTGT GGGCTCTGCC ATGGCTGGTA GCATAGGAGG CTACAACGCC 
CATGCTGCCA ACATCGTCAC TGCCATCTAC ATTGCATGTG GCCAGGATGC AGCACAGAAT 
GTGGGGAGTT CAAACTGTAT TACGTTAATG GAAGCAAGTG GTCCCACAAA TGAAGACTTA 
TACATCAGCT GTACCATGCC GTCTATAGAG ATCGGAACCG TGGGTGGTGG GACCAACCTT 
CTACCTCAGC AAGCCTGCCT GCAGATGCTA GGTGTTCAAG GGGCGTGCAA AGACAATCCT 
GGAGAAAATG CACGGCAGCT TGCCCGAATT GTGTGTGGCA CTGTGATGGC TGGTGAGTTG 
TCCTTGATGG CAGCATTGGC AGCAGGACAT CTTGTCAGAA GTCACATGGT TCACAACAGA 
TCAAAGATAA ATTTACAAGA TCTGCAGGGA ACATGCACCA AGAAGGCAGC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006231888.1
CDS	427..3120
Translation	MRGSKDCVATMLSRLFRMHGLFVASHPWEVIVGTVTLTICMMSMNMFTGNNKICGWNYECPKFEEDVLSSDIIILTITRCIAILYIYFQFQNLRQLGSKYILGIAGLFTIFSSFVFSTVVIHFLDKELTGLNEALPFFLLLIDLSRASALAKFALSSNSQDEVRENIARGMAILGPTFTLDALVECLVIGVGTMSGVRQLEIMCCFGCMSVLANYFVFMTFFPACVSLVLELSRESREGRPIWQLSHFARVLEEEENKPNPVTQRVKMIMSLGLVLVHAHSRWIADPSPQNSTAEQSKVSLGLAEDVSKRIEPSVSLWQFYLSKMISMDIEQVITLSLALLLAVKYIFFEQAETESTLSLKNPITSPVVTPKKAQDNCCRREPLLVRRNQKLSSVEEDPGVNQDRKVEVIKPLVAEAETSGRATFVLGASAASPPLALGAQEPGIELPSEPRPNEECLQILESAEKGAKFLSDAEIIQLVNAKHIPAYKLETLMETHERGVSIRRQLLSAKLAEPSSLQYLPYRDYNYSLVMGACCENVIGYMPIPVGVAGPLCLDGKEYQVPMATTEGCLVASTNRGCRAISLGGGASSRVLADGMSRGPVVRLPRACDSAEVKSWLETPEGFAVVKEAFDSTSRFARLQKLHVTLAGRNLYIRLQSKTGDAMGMNMISKGTEKALLKLQEFFPELQILAVSGNYCTDKKPAAINWIEGRGKTVVCEAVIPAKVVREVLKTTTEAMVDVNINKNLVGSAMAGSIGGYNAHAANIVTAIYIACGQDAAQNVGSSNCITLMEASGPTNEDLYISCTMPSIEIGTVGGGTNLLPQQACLQMLGVQGACKDNPGENARQLARIVCGTVMAGELSLMAALAAGHLVRSHMVHNRSKINLQDLQGTCTKKAA

Target ORF information:

RefSeq Version	XM_006231826.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus 3-hydroxy-3-methylglutaryl-CoA reductase (Hmgcr), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006231826.3

ORF Insert Sequence:

ATGCGAGGAT CCAAGGACTG TGTAGCTACA ATGTTGTCAA GACTTTTCCG TATGCATGGC 
CTCTTTGTGG CCTCCCATCC CTGGGAGGTA ATTGTGGGAA CGGTGACACT TACTATCTGT 
ATGATGTCCA TGAACATGTT CACCGGCAAC AACAAGATCT GTGGTTGGAA TTATGAGTGC 
CCAAAATTTG AAGAGGACGT GCTGAGCAGC GACATCATCA TCCTCACGAT AACCCGGTGC 
ATCGCCATCC TGTACATCTA CTTCCAGTTC CAGAACCTGC GTCAGCTTGG GTCAAAGTAC 
ATTTTGGGTA TTGCCGGCCT CTTCACAATT TTCTCAAGTT TCGTCTTCAG CACTGTCGTC 
ATTCATTTCC TCGACAAAGA ATTGACAGGC TTAAATGAAG CTTTGCCCTT TTTTCTGCTC 
TTGATTGACC TTTCTAGAGC GAGTGCATTG GCCAAGTTTG CCCTGAGTTC AAACTCACAG 
GATGAAGTAA GGGAGAATAT AGCGCGTGGG ATGGCGATCC TGGGCCCCAC GTTCACCCTT 
GACGCTCTGG TGGAATGTCT TGTGATTGGA GTTGGCACCA TGTCAGGGGT GCGGCAGCTT 
GAGATCATGT GCTGCTTTGG CTGTATGTCC GTGCTTGCCA ACTACTTTGT CTTCATGACA 
TTCTTCCCAG CCTGCGTGTC CCTGGTCCTA GAGCTTTCTC GGGAAAGCCG TGAGGGTCGT 
CCAATTTGGC AGCTCAGCCA TTTTGCCAGA GTTTTAGAAG AAGAAGAGAA TAAACCAAAC 
CCAGTAACCC AAAGGGTCAA GATGATCATG TCTTTAGGCC TGGTTCTTGT TCACGCTCAC 
AGTCGCTGGA TAGCTGATCC TTCTCCTCAG AACAGCACAG CAGAACAGTC TAAGGTTTCC 
TTGGGTCTGG CTGAAGATGT GTCCAAGAGA ATTGAGCCGA GTGTTTCTCT CTGGCAGTTT 
TACCTCTCCA AGATGATCAG CATGGACATC GAGCAAGTGA TTACCCTGAG CTTAGCGTTG 
CTTTTGGCTG TCAAGTATAT TTTCTTTGAA CAAGCAGAGA CAGAATCAAC ACTCTCATTA 
AAAAATCCTA TCACATCTCC TGTCGTGACC CCAAAGAAAG CTCAAGACAA CTGTTGTAGA 
CGTGAGCCTC TGCTTGTGAG GAGGAACCAG AAGCTTTCGT CAGTGGAGGA GGATCCAGGA 
GTGAACCAAG ACAGAAAAGT TGAGGTTATA AAACCTTTAG TGGCAGAAGC CGAGACTTCG 
GGCAGAGCTA CGTTTGTGCT TGGCGCCTCT GCAGCCAGCC CTCCATTGGC CCTGGGGGCA 
CAGGAGCCTG GGATCGAACT CCCCAGCGAG CCTCGACCTA ATGAAGAGTG TCTACAGATA 
CTGGAGAGTG CAGAGAAAGG TGCGAAGTTC CTTAGTGATG CAGAGATCAT CCAGTTGGTC 
AATGCTAAGC ACATCCCAGC CTACAAACTG GAAACCCTCA TGGAGACGCA CGAGCGTGGT 
GTGTCTATTC GCCGGCAGCT CCTCTCCGCC AAGCTTGCAG AGCCATCTTC TCTGCAGTAC 
CTGCCTTACA GAGACTATAA TTACTCCTTG GTGATGGGAG CTTGCTGTGA GAACGTGATC 
GGATATATGC CCATCCCTGT TGGAGTGGCA GGACCTCTGT GCCTGGATGG AAAAGAGTAC 
CAGGTGCCAA TGGCAACAAC AGAAGGTTGT CTTGTGGCCA GCACGAACAG AGGCTGCAGA 
GCGATCAGTC TTGGTGGAGG TGCCAGCAGC CGGGTCCTTG CAGATGGGAT GAGCCGAGGC 
CCAGTGGTGC GTCTTCCTCG TGCTTGTGAC TCAGCAGAGG TGAAGAGCTG GCTTGAAACA 
CCTGAAGGGT TTGCAGTGGT AAAGGAGGCC TTCGACAGCA CGAGCAGATT TGCACGTCTA 
CAGAAACTTC ACGTGACGCT GGCAGGACGC AACCTCTACA TCCGTCTCCA GTCCAAAACG 
GGGGACGCCA TGGGGATGAA CATGATTTCC AAGGGTACGG AGAAAGCACT TCTGAAGCTT 
CAAGAGTTCT TTCCTGAGCT GCAGATTCTG GCGGTCAGTG GTAACTATTG CACCGACAAG 
AAACCTGCTG CCATAAACTG GATCGAAGGG AGAGGAAAGA CTGTGGTTTG TGAAGCTGTC 
ATTCCAGCCA AGGTGGTGAG AGAAGTATTA AAGACGACTA CGGAAGCTAT GGTTGACGTA 
AACATTAACA AGAATCTTGT GGGCTCTGCC ATGGCTGGTA GCATAGGAGG CTACAACGCC 
CATGCTGCCA ACATCGTCAC TGCCATCTAC ATTGCATGTG GCCAGGATGC AGCACAGAAT 
GTGGGGAGTT CAAACTGTAT TACGTTAATG GAAGCAAGTG GTCCCACAAA TGAAGACTTA 
TACATCAGCT GTACCATGCC GTCTATAGAG ATCGGAACCG TGGGTGGTGG GACCAACCTT 
CTACCTCAGC AAGCCTGCCT GCAGATGCTA GGTGTTCAAG GGGCGTGCAA AGACAATCCT 
GGAGAAAATG CACGGCAGCT TGCCCGAATT GTGTGTGGCA CTGTGATGGC TGGTGAGTTG 
TCCTTGATGG CAGCATTGGC AGCAGGACAT CTTGTCAGAA GTCACATGGT TCACAACAGA 
TCAAAGATAA ATTTACAAGA TCTGCAGGGA ACATGCACCA AGAAGGCAGC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Regulation of hepatic hydroxy methyl glutarate - CoA reductase for controlling hypercholesterolemia in rats.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie951242-1250(2017 Nov)
Khamis AA,Salama AF,Kenawy ME,Mohamed TM

Adverse effects of long-term exposure to bisphenol A during adulthood leading to hyperglycaemia and hypercholesterolemia in mice.
Toxicology325133-43(2014 Nov)
Marmugi A,Lasserre F,Beuzelin D,Ducheix S,Huc L,Polizzi A,Chetivaux M,Pineau T,Martin P,Guillou H,Mselli-Lakhal L

Involvement of hepatic IL-1 in the strain-dependent sex differences in serum total cholesterol levels in rats.
Biological & pharmaceutical bulletin37(4)654-8(2014)
Kumagai Y,Sekimoto M,Okamoto M,Kurita R,Kojima M,Degawa M

Effects of simvaglyzin and atorvaglyzin on the expression of 3-hydroxy-3-methyl-glutaryl-CoA reductase in rat liver.
Bulletin of experimental biology and medicine156(1)63-5(2013 Nov)
Stakhneva EM,Vavilin VA,Ragino YI,Safronova OG,Shintyapina AB,Ivanova MV

Hypocholesterolemic effect of capsaicinoids in rats fed diets with or without cholesterol.
Journal of agricultural and food chemistry61(18)4287-93(2013 May)
Zhang L,Fang G,Zheng L,Chen Z,Liu X

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オンライン注文

Hmgcr cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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