hmg1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	hmg1
Entrez Gene ID	2538999
Full Name	3-hydroxy-3-methylglutaryl-CoA reductase Hmg1
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001023225.2	NP_588235.1	3-hydroxy-3-methylglutaryl-CoA reductase Hmg1

Pathways from BioSystems

Homologies

Homo sapiens (human)	HMGCR	NP_000850.1
Gallus gallus (chicken)	HMGCR	NP_989816.1
Caenorhabditis elegans (roundworm)	hmgr-1	NP_498626.2
Saccharomyces cerevisiae (baker's yeast)	HMG2	NP_013555.1
Kluyveromyces lactis	KLLA0B04642g	XP_451740.1
Pan troglodytes (chimpanzee)	HMGCR	XP_001148405.2
Bos taurus (cattle)	HMGCR	NP_001099083.1
Xenopus tropicalis (tropical clawed frog)	hmgcr	XP_004910453.1
	HMGCR	XP_002804463.1
Canis lupus familiaris (dog)	HMGCR	XP_536323.3
Drosophila melanogaster (fruit fly)	Hmgcr	NP_732900.1
Magnaporthe oryzae (rice blast fungus)	MGG_08975	XP_003714014.1
Neurospora crassa	NCU00712	XP_964546.1
Arabidopsis thaliana (thale cress)	HMG1	NP_177775.2
Oryza sativa (rice)	Os09g0492700	NP_001063541.1
Oryza sativa (rice)	Os08g0512700	NP_001062221.1
Mus musculus (house mouse)	Hmgcr	NP_032281.2
Rattus norvegicus (Norway rat)	Hmgcr	NP_037266.2
Danio rerio (zebrafish)	hmgcra	NP_001073446.1
Arabidopsis thaliana (thale cress)	HMG2	NP_179329.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002288	XP_307890.5
Saccharomyces cerevisiae (baker's yeast)	HMG1	NP_013636.1
Eremothecium gossypii	AGOS_AER152W	NP_985010.2
Schizosaccharomyces pombe (fission yeast)	hmg1	NP_588235.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This is the first demonstration of clear phenotypes arising from a nonsense mutation of an essential gene, HMG1, in fission yeast.
Title: Pleiotropic phenotypes caused by an opal nonsense mutation in an essential gene encoding HMG-CoA reductase in fission yeast.

Intracellular Signaling Peptides and Proteins dependent regulation of 3-hydroxy-3-methylglutaryl-CoA reductase activity functions through the phosphorylation of S1024 and T1028.
Title: Insig regulates HMG-CoA reductase by controlling enzyme phosphorylation in fission yeast.

The following hmg1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the hmg1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc99289	NM_001023225.2 Latest version!	Schizosaccharomyces pombe 3-hydroxy-3-methylglutaryl-CoA reductase Hmg1 (hmg1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

hmg1 ( NM_001023225.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_588235.1 Schizosaccharomyces pombe 3-hydroxy-3-methylglutaryl-CoA reductase Hmg1 (hmg1), mRNA.

Price & Availability↑

CloneID	OSc99289
Clone ID Related Accession (Same CDS sequence)	NM_001023225.2
Accession Version	NM_001023225.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3162bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	3-hydroxy-3-methylglutaryl-CoA reductase Hmg1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003421). On Dec 10, 2012 this sequence version replaced NM_001023225.1.

ATGATTTATA AACTTGCTGC TCGGTATCCA ATTCAAGTGA TTGCCATTGT GGGCATCCTT 
GTCTCTATGG CATATTTTTC CTTCTTGGAA GCCTTAACTC AAGAAGATTT CCCAGTATTA 
ATTCGTGCTT TAAAACGATT TGGTATTTTG GATGGATTTC CGAACACTCG TCTACCTAAC 
GAGATGATTT TAAAGCTAAG TAGTGTCCAA GGTGAGGATG CCTCGGTTTG GGAACAAATT 
CCTGCCGCAG AGTTGGGTGG AGAAGGATTT GTTGATTTTG ATATCACCCA ATGGTATTAC 
CCTGCGAATG CAAAGGTCGA TGTTGCTCAA TTGGTAGAGC CATATCGTAA TGACTGCATT 
TTCCATGACG CTTCCGGTGC TTGCCATTTC TTTTTTAAAG AGGTCGGCAA TTGGACTGTA 
TCTAGTATTG CATTACCATC GAATCTCGCC AATCCGCCCA TCGATTACTT CCTTGACAGT 
TCTAGTACTG TCATTCAAAG AATTCTTCCT GCAATTCGTG AACACGGCAT TTCATGGTCG 
TGGTTACTTC AGTTGATCGC TCGCACTTGG ATGAATACTT TAAAGATCGC TAGCCAAGCT 
TCTAAGACCG AACTGTTAAT TGTTGGTACT GCTTATGCTT GCATGTTGAT ATCCATTGTG 
TCTCTTTACC TTAAGATGCG TCGTCTAGGA TCCAAATTTT GGCTATTTTT TAGTGTCCTG 
CTTTCCACTT TGTTCTCTGT TCAATTCGCA ATGACTTTGG TACGTGCATC TGGTGTACGG 
ATTAGCCTTG TCTCGTTGAT TGAAAGTTTG CCTTTTCTGA TAAATGTGGT TGCCCTTGAT 
AAAGCTGCAG AATTAACGCG GCAAGTTATC ACTCGATGCT CTGTCTCTGA CTCCCATTCT 
CCTATGCATG AAGATATTGC AAAGGCTTGC CGCAACGCTG CACCGCCTAT CCTCCGTCAT 
TTTTCTTTTG GAATAGTCGT TTTGGCAATT TTTTCGTATT GTAATTTTGG TATCAAACAA 
TTCTTTTTGT TTGCTGCTGT CATGATTTAT GATTTACTCT TACTGTTCTC TTTCTTTGTC 
GCCATACTCA CCCTGAAACT TGAGATGCGC CGCTATAATG CTAAGGATGA TGTTCGAAAG 
GTTCTTATAG AAGAGGGTCT CTCTGAGTCT ACGGCTCGTC ATGTTGCAGA CGGCAACGAT 
TCTTCGGCTA CTACTTCTGC AGGGTCACGG TATTTTAAAG TTAGATATGG AACCAAAATC 
ATTCTTTTCA TATTCATTGC CTTCAATTTG TTTGAACTTT GTTCTATTCC GTTCAAACAT 
TATGCTGCCA CTTCTGCTGC TGCTGCTCGA TTAATTCCTC TTGTTCGCTC TCAATATCCC 
GATTTTAAGT CACAAAGGCT TTTGGATGAT GGAGTGTTTG ACGACGTTCT TTCTGCTATT 
TCTTCAATGT CCAACATTGA ATCACCAAGC GTCCGTTTAC TACCAGCTGT TTTTTACGGT 
GCCGAATTAT CCAGCACTTC TTTTCTATCT ACTATCCATT CGTTTATTAA CAACTGGTCT 
CACTACATCA GTGCTTCGTT TTTGTCAAAA TGGATCGTCT GTGCTTTATC TTTGAGTATT 
GCCGTCAACG TTTTTCTTTT GAACGCTGCT CGATTGAACT CCATCAAAGA GGAACCTGAG 
AAAAAGGTGG TTGAAAAAGT TGTTGAAGTC GTTAAGTATA TTCCTTCGTC GAATAGTTCA 
TCCATTGATG ACATTCAGAA AGACGAAATC GCTCAAGAAT CCGTTGTACG TTCGCTTGAG 
GAATGTATCA CTCTTTACAA CAATGGTCAA ATTTCAACTT TAAATGATGA AGAAGTTGTG 
CAGTTAACCT TAGCTAAAAA AATCCCTCTT TATGCTTTAG AACGAGTACT TAAAGACGTT 
ACCAGAGCTG TTGTAATTCG CCGAACGGTC GTCTCCCGTT CATCTAGGAC GAAGACACTT 
GAAAGCTCTA ATTGCCCCGT CTATCATTAC GATTACTCTA GGGTTTTGAA TGCATGTTGT 
GAAAACGTTA TAGGTTACAT GCCCTTGCCC CTTGGTGTAG CTGGTCCTCT CATTATTGAC 
GGTAAGCCTT TTTATATCCC TATGGCTACT ACTGAGGGTG CATTGGTTGC TTCTACTATG 
CGTGGATGCA AAGCTATTAA CGCTGGTGGT GGTGCTGTTA CTGTATTGAC TCGTGATCAA 
ATGTCCCGTG GACCATGTGT GGCTTTCCCG AATTTGACGC GCGCTGGTCG TGCTAAAATT 
TGGTTGGATA GCCCTGAAGG CCAAGAGGTT ATGAAGAAGG CTTTTAACTC TACTAGCCGT 
TTTGCTAGAC TTCAACATAT TAAGACTGCT CTAGCTGGTA CTCGTCTTTT CATCCGTTTC 
TGTACGTCTA CCGGTGATGC TATGGGTATG AATATGATTT CCAAAGGTGT TGAGCATGCA 
CTTGTAGTAA TGAGCAATGA CGCTGGTTTT GATGACATGC AAGTGATCAG TGTATCAGGG 
AATTATTGTA CAGATAAGAA GCCTGCTGCT ATTAATTGGA TTGATGGTCG TGGTAAAAGT 
GTTATTGCTG AAGCTATTAT TCCTGGTGAT GCTGTCAAAT CGGTATTGAA AACTACTGTT 
GAAGATTTGG TTAAATTAAA TGTTGACAAA AACCTCATTG GTAGTGCTAT GGCTGGCAGC 
GTTGGAGGAT TTAATGCTCA TGCTGCTAAC ATTGTCACGG CAGTATATTT AGCAACTGGT 
CAAGACCCTG CCCAAAATGT TGAAAGCAGT AATTGTATAA CTTTGATGGA TAATGTTGAT 
GGTAACCTTC AGCTTAGCGT GTCAATGCCA TCCATTGAAG TTGGAACAAT TGGTGGTGGA 
ACGGTTTTGG AACCGCAAGG TGCTATGCTT GACTTGCTCG GTGTGCGAGG TGCCCATATG 
ACCTCTCCCG GTGATAATTC TCGTCAACTA GCACGTGTCG TTGCAGCCGC CGTAATGGCT 
GGTGAACTGT CTTTGTGTTC CGCACTTGCT AGTGGCCATT TAGTTAAATC CCATATCGGA 
CTCAATCGAA GTGCGCTGAA TACTCCTGCT ATGGACTCTT CTGCCAAGAA ACCAGCGACT 
GATGCTCTAA AATCCGTTAA CTCTCGAGTA CCGGGACGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_588235.1
CDS	803..3964
Translation	MIYKLAARYPIQVIAIVGILVSMAYFSFLEALTQEDFPVLIRALKRFGILDGFPNTRLPNEMILKLSSVQGEDASVWEQIPAAELGGEGFVDFDITQWYYPANAKVDVAQLVEPYRNDCIFHDASGACHFFFKEVGNWTVSSIALPSNLANPPIDYFLDSSSTVIQRILPAIREHGISWSWLLQLIARTWMNTLKIASQASKTELLIVGTAYACMLISIVSLYLKMRRLGSKFWLFFSVLLSTLFSVQFAMTLVRASGVRISLVSLIESLPFLINVVALDKAAELTRQVITRCSVSDSHSPMHEDIAKACRNAAPPILRHFSFGIVVLAIFSYCNFGIKQFFLFAAVMIYDLLLLFSFFVAILTLKLEMRRYNAKDDVRKVLIEEGLSESTARHVADGNDSSATTSAGSRYFKVRYGTKIILFIFIAFNLFELCSIPFKHYAATSAAAARLIPLVRSQYPDFKSQRLLDDGVFDDVLSAISSMSNIESPSVRLLPAVFYGAELSSTSFLSTIHSFINNWSHYISASFLSKWIVCALSLSIAVNVFLLNAARLNSIKEEPEKKVVEKVVEVVKYIPSSNSSSIDDIQKDEIAQESVVRSLEECITLYNNGQISTLNDEEVVQLTLAKKIPLYALERVLKDVTRAVVIRRTVVSRSSRTKTLESSNCPVYHYDYSRVLNACCENVIGYMPLPLGVAGPLIIDGKPFYIPMATTEGALVASTMRGCKAINAGGGAVTVLTRDQMSRGPCVAFPNLTRAGRAKIWLDSPEGQEVMKKAFNSTSRFARLQHIKTALAGTRLFIRFCTSTGDAMGMNMISKGVEHALVVMSNDAGFDDMQVISVSGNYCTDKKPAAINWIDGRGKSVIAEAIIPGDAVKSVLKTTVEDLVKLNVDKNLIGSAMAGSVGGFNAHAANIVTAVYLATGQDPAQNVESSNCITLMDNVDGNLQLSVSMPSIEVGTIGGGTVLEPQGAMLDLLGVRGAHMTSPGDNSRQLARVVAAAVMAGELSLCSALASGHLVKSHIGLNRSALNTPAMDSSAKKPATDALKSVNSRVPGR

Target ORF information:

RefSeq Version	NM_001023225.2
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe 3-hydroxy-3-methylglutaryl-CoA reductase Hmg1 (hmg1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001023225.2

ORF Insert Sequence:

ATGATTTATA AACTTGCTGC TCGGTATCCA ATTCAAGTGA TTGCCATTGT GGGCATCCTT 
GTCTCTATGG CATATTTTTC CTTCTTGGAA GCCTTAACTC AAGAAGATTT CCCAGTATTA 
ATTCGTGCTT TAAAACGATT TGGTATTTTG GATGGATTTC CGAACACTCG TCTACCTAAC 
GAGATGATTT TAAAGCTAAG TAGTGTCCAA GGTGAGGATG CCTCGGTTTG GGAACAAATT 
CCTGCCGCAG AGTTGGGTGG AGAAGGATTT GTTGATTTTG ATATCACCCA ATGGTATTAC 
CCTGCGAATG CAAAGGTCGA TGTTGCTCAA TTGGTAGAGC CATATCGTAA TGACTGCATT 
TTCCATGACG CTTCCGGTGC TTGCCATTTC TTTTTTAAAG AGGTCGGCAA TTGGACTGTA 
TCTAGTATTG CATTACCATC GAATCTCGCC AATCCGCCCA TCGATTACTT CCTTGACAGT 
TCTAGTACTG TCATTCAAAG AATTCTTCCT GCAATTCGTG AACACGGCAT TTCATGGTCG 
TGGTTACTTC AGTTGATCGC TCGCACTTGG ATGAATACTT TAAAGATCGC TAGCCAAGCT 
TCTAAGACCG AACTGTTAAT TGTTGGTACT GCTTATGCTT GCATGTTGAT ATCCATTGTG 
TCTCTTTACC TTAAGATGCG TCGTCTAGGA TCCAAATTTT GGCTATTTTT TAGTGTCCTG 
CTTTCCACTT TGTTCTCTGT TCAATTCGCA ATGACTTTGG TACGTGCATC TGGTGTACGG 
ATTAGCCTTG TCTCGTTGAT TGAAAGTTTG CCTTTTCTGA TAAATGTGGT TGCCCTTGAT 
AAAGCTGCAG AATTAACGCG GCAAGTTATC ACTCGATGCT CTGTCTCTGA CTCCCATTCT 
CCTATGCATG AAGATATTGC AAAGGCTTGC CGCAACGCTG CACCGCCTAT CCTCCGTCAT 
TTTTCTTTTG GAATAGTCGT TTTGGCAATT TTTTCGTATT GTAATTTTGG TATCAAACAA 
TTCTTTTTGT TTGCTGCTGT CATGATTTAT GATTTACTCT TACTGTTCTC TTTCTTTGTC 
GCCATACTCA CCCTGAAACT TGAGATGCGC CGCTATAATG CTAAGGATGA TGTTCGAAAG 
GTTCTTATAG AAGAGGGTCT CTCTGAGTCT ACGGCTCGTC ATGTTGCAGA CGGCAACGAT 
TCTTCGGCTA CTACTTCTGC AGGGTCACGG TATTTTAAAG TTAGATATGG AACCAAAATC 
ATTCTTTTCA TATTCATTGC CTTCAATTTG TTTGAACTTT GTTCTATTCC GTTCAAACAT 
TATGCTGCCA CTTCTGCTGC TGCTGCTCGA TTAATTCCTC TTGTTCGCTC TCAATATCCC 
GATTTTAAGT CACAAAGGCT TTTGGATGAT GGAGTGTTTG ACGACGTTCT TTCTGCTATT 
TCTTCAATGT CCAACATTGA ATCACCAAGC GTCCGTTTAC TACCAGCTGT TTTTTACGGT 
GCCGAATTAT CCAGCACTTC TTTTCTATCT ACTATCCATT CGTTTATTAA CAACTGGTCT 
CACTACATCA GTGCTTCGTT TTTGTCAAAA TGGATCGTCT GTGCTTTATC TTTGAGTATT 
GCCGTCAACG TTTTTCTTTT GAACGCTGCT CGATTGAACT CCATCAAAGA GGAACCTGAG 
AAAAAGGTGG TTGAAAAAGT TGTTGAAGTC GTTAAGTATA TTCCTTCGTC GAATAGTTCA 
TCCATTGATG ACATTCAGAA AGACGAAATC GCTCAAGAAT CCGTTGTACG TTCGCTTGAG 
GAATGTATCA CTCTTTACAA CAATGGTCAA ATTTCAACTT TAAATGATGA AGAAGTTGTG 
CAGTTAACCT TAGCTAAAAA AATCCCTCTT TATGCTTTAG AACGAGTACT TAAAGACGTT 
ACCAGAGCTG TTGTAATTCG CCGAACGGTC GTCTCCCGTT CATCTAGGAC GAAGACACTT 
GAAAGCTCTA ATTGCCCCGT CTATCATTAC GATTACTCTA GGGTTTTGAA TGCATGTTGT 
GAAAACGTTA TAGGTTACAT GCCCTTGCCC CTTGGTGTAG CTGGTCCTCT CATTATTGAC 
GGTAAGCCTT TTTATATCCC TATGGCTACT ACTGAGGGTG CATTGGTTGC TTCTACTATG 
CGTGGATGCA AAGCTATTAA CGCTGGTGGT GGTGCTGTTA CTGTATTGAC TCGTGATCAA 
ATGTCCCGTG GACCATGTGT GGCTTTCCCG AATTTGACGC GCGCTGGTCG TGCTAAAATT 
TGGTTGGATA GCCCTGAAGG CCAAGAGGTT ATGAAGAAGG CTTTTAACTC TACTAGCCGT 
TTTGCTAGAC TTCAACATAT TAAGACTGCT CTAGCTGGTA CTCGTCTTTT CATCCGTTTC 
TGTACGTCTA CCGGTGATGC TATGGGTATG AATATGATTT CCAAAGGTGT TGAGCATGCA 
CTTGTAGTAA TGAGCAATGA CGCTGGTTTT GATGACATGC AAGTGATCAG TGTATCAGGG 
AATTATTGTA CAGATAAGAA GCCTGCTGCT ATTAATTGGA TTGATGGTCG TGGTAAAAGT 
GTTATTGCTG AAGCTATTAT TCCTGGTGAT GCTGTCAAAT CGGTATTGAA AACTACTGTT 
GAAGATTTGG TTAAATTAAA TGTTGACAAA AACCTCATTG GTAGTGCTAT GGCTGGCAGC 
GTTGGAGGAT TTAATGCTCA TGCTGCTAAC ATTGTCACGG CAGTATATTT AGCAACTGGT 
CAAGACCCTG CCCAAAATGT TGAAAGCAGT AATTGTATAA CTTTGATGGA TAATGTTGAT 
GGTAACCTTC AGCTTAGCGT GTCAATGCCA TCCATTGAAG TTGGAACAAT TGGTGGTGGA 
ACGGTTTTGG AACCGCAAGG TGCTATGCTT GACTTGCTCG GTGTGCGAGG TGCCCATATG 
ACCTCTCCCG GTGATAATTC TCGTCAACTA GCACGTGTCG TTGCAGCCGC CGTAATGGCT 
GGTGAACTGT CTTTGTGTTC CGCACTTGCT AGTGGCCATT TAGTTAAATC CCATATCGGA 
CTCAATCGAA GTGCGCTGAA TACTCCTGCT ATGGACTCTT CTGCCAAGAA ACCAGCGACT 
GATGCTCTAA AATCCGTTAA CTCTCGAGTA CCGGGACGTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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hmg1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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