Hmgcr cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hmgcr
Entrez Gene ID	42803
Full Name	HMG Coenzyme A reductase
Synonyms	BcDNA:LD15354,CG10367,Clb,DmHMG-CoAr,Dmel\CG10367,HMGCR,HMGCR_Dm,HMGCoAR,HMGCoARr,HMGCoAr,HMGR,HmG-CoAR,Hmg,bro1,bs32f05.y1,clb,hmgc,hmgcr,hmgrc,l(3)01152,l(3)04684
General protein information	Preferred Names HMG Coenzyme A reductase Names CG10367 gene product from transcript CG10367-RA 3-Hydroxy-3-Methylglutaryl CoA reductase 3-hydroxy-3-methylglutaryl coenzyme A reductase CG10367-PA HMG coenzyme A reductase HMG-CoA reductase HMG-CoA reductase class I HMGCo-A reductase HMGCoA reductase Hmg-CoA-reductase Hmgcr-PA columbus lethal (3) 04684
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 95B1-95B3\|3-80.0 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_170089.2	NP_732900.1	HMG coenzyme A reductase

Pathways from BioSystems

Homologies

Homo sapiens (human)	HMGCR	NP_000850.1
Gallus gallus (chicken)	HMGCR	NP_989816.1
Caenorhabditis elegans (roundworm)	hmgr-1	NP_498626.2
Saccharomyces cerevisiae (baker's yeast)	HMG2	NP_013555.1
Kluyveromyces lactis	KLLA0B04642g	XP_451740.1
Pan troglodytes (chimpanzee)	HMGCR	XP_001148405.2
Bos taurus (cattle)	HMGCR	NP_001099083.1
Xenopus tropicalis (tropical clawed frog)	hmgcr	XP_004910453.1
	HMGCR	XP_002804463.1
Canis lupus familiaris (dog)	HMGCR	XP_536323.3
Drosophila melanogaster (fruit fly)	Hmgcr	NP_732900.1
Magnaporthe oryzae (rice blast fungus)	MGG_08975	XP_003714014.1
Neurospora crassa	NCU00712	XP_964546.1
Arabidopsis thaliana (thale cress)	HMG1	NP_177775.2
Oryza sativa (rice)	Os09g0492700	NP_001063541.1
Oryza sativa (rice)	Os08g0512700	NP_001062221.1
Mus musculus (house mouse)	Hmgcr	NP_032281.2
Rattus norvegicus (Norway rat)	Hmgcr	NP_037266.2
Danio rerio (zebrafish)	hmgcra	NP_001073446.1
Arabidopsis thaliana (thale cress)	HMG2	NP_179329.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002288	XP_307890.5
Saccharomyces cerevisiae (baker's yeast)	HMG1	NP_013636.1
Eremothecium gossypii	AGOS_AER152W	NP_985010.2
Schizosaccharomyces pombe (fission yeast)	hmg1	NP_588235.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The specificity and evolutionary conservation of the HMGCoAr pathway for germ cells suggest that an attractant common to invertebrates and vertebrates guides germ cells in early embryos.
Title: Isoprenoids control germ cell migration downstream of HMGCoA reductase.

The following Hmgcr gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hmgcr cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa05824	NM_170089.2 Latest version!	Drosophila melanogaster HMG coenzyme A reductase (Hmgcr), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hmgcr ( NM_170089.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_732900.1 Drosophila melanogaster HMG coenzyme A reductase (Hmgcr), mRNA.

Price & Availability↑

CloneID	OFa05824
Clone ID Related Accession (Same CDS sequence)	NM_170089.2
Accession Version	NM_170089.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2763bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	HMG coenzyme A reductase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_170089.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGATAGGAC GTTTGTTTCG CGCCCACGGC GAGTTCTGCG CCTCGCATCC CTGGGAGGTC 
ATCGTGGCCC TGCTGACCAT TACGGCATGC ATGCTCACGG TGGACAAGAA CAATACCCTG 
GATGCGAGCA GCGGATTGGG CACAGCCACC GCCTCAGCTG CCGCCGCCGG AGGATCCGGA 
TCTGGAGCTG GATCTGGAGC AAGTGGAACA ATACCACCAT CGTCTATGGG TGGCTCGGCC 
ACCTCCAGCC GGCACAGGCC CTGTCACGGT TGGAGCCAAT CCTGTGATGG CCTAGAGGCC 
GAATACAATG CAGCCGACGT AATCCTGATG ACCATCGTCC GCTGCACTGC CGTACTGTAC 
TGCTACTACC AGTTCTGCAG CCTCCACCGC CTGGGATCCA AATATGTGCT GGGCATTGCC 
GGCCTCTTCA CGGTCTTCTC CAGCTTCATC TTCACGACGG CCATCATCAA GTTCCTGGGC 
AGCGACATCT CCGAACTGAA GGACGCCCTG TTCTTCCTGC TGCTGGTCAT TGATCTGTCC 
AACTCTGGCC GCCTGGCGCA GCTGGCGCTA TCGGGCAGTA ACCAAGCGGA GGTGACGCAG 
AACATTGCAA GGGGACTGGA GCTCCTGGGA CCAGCCATCT CCCTGGACAC AATTGTGGAG 
GTGCTGCTGG TGGGCGTTGG CACACTGTCG GGTGTACAGC GTCTGGAGGT ACTGTGCATG 
TTTGCCGTGC TCTCGGTGCT GGTTAACTAT GTGGTCTTTA TGACCTTTTA TCCCGCCTGC 
CTTTCGCTAA TCTTCGATCT ATCACGCTCC GGCGTTGATA TGTCTGTGGT GCGTGAAAAA 
GCGAAGGGCT CGCTGCTCCT CAAGTCGCTA ACAGAGGAGG AGCAGAAGGC CAATCCAGTG 
CTGCAGCGTG TTAAGCTTAT CATGACCACA GGCCTGATGG CGGTGCACAT TTATAGCCGG 
GTGGCTTTCT CCGGCAGCGA CTACGATGCC GTGGACAAGA CGCTGACGCC CACGCTAAGC 
CTGAATGTGA GCAATAATCG CACGGAATCG GGTGAAATCG CCGACATTAT CATCAAATGG 
CTGACCATGA GTGCCGATCA CATAGTAATC TCAATTGTTC TCATCGCCTT GGTAGTTAAA 
TTTATTTGCT TTGACAATCG TGATCCTCTG CCGGATCAGT TGCGTCAATC AGGTCCAGTT 
GCCATCGCCG CCAAGGCTTC ACAAACCACA CCAATTGATG AAGAGCACGT TGAACAGGAA 
AAGGATACAG AAAATTCAGC TGCAGTCCGC ACACTATTAT TTACCATTGA AGATCAGAGT 
TCAGCAAATG CTTCAACTCA GACGGATCTG CTGCCACTGC GCCACCGACT GGTGGGTCCC 
ATAAAGCCTC CACGCCCAGT TCAGGAATGC CTTGACATCC TTAACTCCAC TGAAGAGGGC 
AGTGGACCAG CGGCCTTGAG TGACGAGGAG ATTGTGTCTA TTGTCCATGC CGGTGGCACT 
CACTGCCCAC TCCACAAAAT AGAATCTGTA CTAGATGATC CCGAGAGGGG AGTGCGAATC 
CGCCGACAGA TCATTGGCAG TCGTGCCAAG ATGCCGGTTG GTCGACTGGA TGTCTTGCCT 
TATGAACACT TTGACTACCG CAAAGTGCTG AACGCCTGCT GTGAGAACGT TCTGGGCTAC 
GTTCCCATTC CCGTTGGCTA CGCAGGACCC CTGCTGTTAG ACGGAGAGAC CTACTACGTG 
CCCATGGCAA CCACCGAGGG TGCTTTGGTT GCATCCACGA ATCGTGGCTG CAAAGCGCTC 
TCCGTTCGTG GTGTTAGATC TGTCGTGGAG GACGTGGGCA TGACCCGTGC TCCGTGTGTA 
AGATTCCCCA GCGTTGCCCG CGCGGCCGAA GCAAAATCTT GGATCGAAAA CGACGAAAAC 
TACCGGGTGG TGAAAACTGA ATTCGATTCT ACATCGCGCT TTGGCCGTCT CAAGGACTGC 
CACATTGCCA TGGATGGACC ACAGCTGTAT ATTCGTTTTG TGGCCATTAC CGGCGACGCC 
ATGGGCATGA ACATGGTGTC CAAGGGCGCT GAGATGGCCC TGCGCCGAAT TCAGCTTCAG 
TTTCCCGACA TGCAGATCAT CTCGCTGAGT GGAAACTTCT GCTGCGACAA GAAACCGGCG 
GCCATCAACT GGATTAAGGG ACGTGGCAAG CGGGTGGTCA CCGAGTGTAC CATTTCGGCA 
GCCACACTGC GTTCCGTGCT GAAGACAGAT GCCAAGACAC TGGTCGAATG TAACAAGCTG 
AAGAATATGG GAGGCAGCGC GATGGCCGGT AGCATTGGTG GCAACAATGC TCATGCCGCC 
AATATGGTGA CTGCCGTCTT TTTGGCCACG GGGCAGGATC CCGCCCAGAA TGTCACCTCG 
AGTAATTGCT CCACTGCCAT GGAGTGCTGG GCGGAGAACA GCGAGGATCT TTACATGACC 
TGCACAATGC CTTCGCTGGA GGTGGGAACT GTGGGCGGCG GAACCGGGTT GCCCGGCCAG 
AGCGCATGCC TGGAAATGCT CGGAGTCCGC GGAGCTCACG CCACGCGGCC AGGAGACAAC 
GCCAAGAAGC TGGCACAGAT CGTCTGCGCC ACGGTTATGG CCGGCGAATT GAGCCTGATG 
GCGGCGTTGG TCAATAGCGA TCTAGTCAAG AGTCATATGC GGCACAATCG ATCCTCCATC 
GCAGTGAACA GCGCCAACAA TCCTCTCAAC GTGACCGTGT CCAGCTGCAG CACCATCAGC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_732900.1
CDS	563..3325
Translation	MIGRLFRAHGEFCASHPWEVIVALLTITACMLTVDKNNTLDASSGLGTATASAAAAGGSGSGAGSGASGTIPPSSMGGSATSSRHRPCHGWSQSCDGLEAEYNAADVILMTIVRCTAVLYCYYQFCSLHRLGSKYVLGIAGLFTVFSSFIFTTAIIKFLGSDISELKDALFFLLLVIDLSNSGRLAQLALSGSNQAEVTQNIARGLELLGPAISLDTIVEVLLVGVGTLSGVQRLEVLCMFAVLSVLVNYVVFMTFYPACLSLIFDLSRSGVDMSVVREKAKGSLLLKSLTEEEQKANPVLQRVKLIMTTGLMAVHIYSRVAFSGSDYDAVDKTLTPTLSLNVSNNRTESGEIADIIIKWLTMSADHIVISIVLIALVVKFICFDNRDPLPDQLRQSGPVAIAAKASQTTPIDEEHVEQEKDTENSAAVRTLLFTIEDQSSANASTQTDLLPLRHRLVGPIKPPRPVQECLDILNSTEEGSGPAALSDEEIVSIVHAGGTHCPLHKIESVLDDPERGVRIRRQIIGSRAKMPVGRLDVLPYEHFDYRKVLNACCENVLGYVPIPVGYAGPLLLDGETYYVPMATTEGALVASTNRGCKALSVRGVRSVVEDVGMTRAPCVRFPSVARAAEAKSWIENDENYRVVKTEFDSTSRFGRLKDCHIAMDGPQLYIRFVAITGDAMGMNMVSKGAEMALRRIQLQFPDMQIISLSGNFCCDKKPAAINWIKGRGKRVVTECTISAATLRSVLKTDAKTLVECNKLKNMGGSAMAGSIGGNNAHAANMVTAVFLATGQDPAQNVTSSNCSTAMECWAENSEDLYMTCTMPSLEVGTVGGGTGLPGQSACLEMLGVRGAHATRPGDNAKKLAQIVCATVMAGELSLMAALVNSDLVKSHMRHNRSSIAVNSANNPLNVTVSSCSTIS

Target ORF information:

RefSeq Version	NM_170089.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster HMG coenzyme A reductase (Hmgcr), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170089.2

ORF Insert Sequence:

ATGATAGGAC GTTTGTTTCG CGCCCACGGC GAGTTCTGCG CCTCGCATCC CTGGGAGGTC 
ATCGTGGCCC TGCTGACCAT TACGGCATGC ATGCTCACGG TGGACAAGAA CAATACCCTG 
GATGCGAGCA GCGGATTGGG CACAGCCACC GCCTCAGCTG CCGCCGCCGG AGGATCCGGA 
TCTGGAGCTG GATCTGGAGC AAGTGGAACA ATACCACCAT CGTCTATGGG TGGCTCGGCC 
ACCTCCAGCC GGCACAGGCC CTGTCACGGT TGGAGCCAAT CCTGTGATGG CCTAGAGGCC 
GAATACAATG CAGCCGACGT AATCCTGATG ACCATCGTCC GCTGCACTGC CGTACTGTAC 
TGCTACTACC AGTTCTGCAG CCTCCACCGC CTGGGATCCA AATATGTGCT GGGCATTGCC 
GGCCTCTTCA CGGTCTTCTC CAGCTTCATC TTCACGACGG CCATCATCAA GTTCCTGGGC 
AGCGACATCT CCGAACTGAA GGACGCCCTG TTCTTCCTGC TGCTGGTCAT TGATCTGTCC 
AACTCTGGCC GCCTGGCGCA GCTGGCGCTA TCGGGCAGTA ACCAAGCGGA GGTGACGCAG 
AACATTGCAA GGGGACTGGA GCTCCTGGGA CCAGCCATCT CCCTGGACAC AATTGTGGAG 
GTGCTGCTGG TGGGCGTTGG CACACTGTCG GGTGTACAGC GTCTGGAGGT ACTGTGCATG 
TTTGCCGTGC TCTCGGTGCT GGTTAACTAT GTGGTCTTTA TGACCTTTTA TCCCGCCTGC 
CTTTCGCTAA TCTTCGATCT ATCACGCTCC GGCGTTGATA TGTCTGTGGT GCGTGAAAAA 
GCGAAGGGCT CGCTGCTCCT CAAGTCGCTA ACAGAGGAGG AGCAGAAGGC CAATCCAGTG 
CTGCAGCGTG TTAAGCTTAT CATGACCACA GGCCTGATGG CGGTGCACAT TTATAGCCGG 
GTGGCTTTCT CCGGCAGCGA CTACGATGCC GTGGACAAGA CGCTGACGCC CACGCTAAGC 
CTGAATGTGA GCAATAATCG CACGGAATCG GGTGAAATCG CCGACATTAT CATCAAATGG 
CTGACCATGA GTGCCGATCA CATAGTAATC TCAATTGTTC TCATCGCCTT GGTAGTTAAA 
TTTATTTGCT TTGACAATCG TGATCCTCTG CCGGATCAGT TGCGTCAATC AGGTCCAGTT 
GCCATCGCCG CCAAGGCTTC ACAAACCACA CCAATTGATG AAGAGCACGT TGAACAGGAA 
AAGGATACAG AAAATTCAGC TGCAGTCCGC ACACTATTAT TTACCATTGA AGATCAGAGT 
TCAGCAAATG CTTCAACTCA GACGGATCTG CTGCCACTGC GCCACCGACT GGTGGGTCCC 
ATAAAGCCTC CACGCCCAGT TCAGGAATGC CTTGACATCC TTAACTCCAC TGAAGAGGGC 
AGTGGACCAG CGGCCTTGAG TGACGAGGAG ATTGTGTCTA TTGTCCATGC CGGTGGCACT 
CACTGCCCAC TCCACAAAAT AGAATCTGTA CTAGATGATC CCGAGAGGGG AGTGCGAATC 
CGCCGACAGA TCATTGGCAG TCGTGCCAAG ATGCCGGTTG GTCGACTGGA TGTCTTGCCT 
TATGAACACT TTGACTACCG CAAAGTGCTG AACGCCTGCT GTGAGAACGT TCTGGGCTAC 
GTTCCCATTC CCGTTGGCTA CGCAGGACCC CTGCTGTTAG ACGGAGAGAC CTACTACGTG 
CCCATGGCAA CCACCGAGGG TGCTTTGGTT GCATCCACGA ATCGTGGCTG CAAAGCGCTC 
TCCGTTCGTG GTGTTAGATC TGTCGTGGAG GACGTGGGCA TGACCCGTGC TCCGTGTGTA 
AGATTCCCCA GCGTTGCCCG CGCGGCCGAA GCAAAATCTT GGATCGAAAA CGACGAAAAC 
TACCGGGTGG TGAAAACTGA ATTCGATTCT ACATCGCGCT TTGGCCGTCT CAAGGACTGC 
CACATTGCCA TGGATGGACC ACAGCTGTAT ATTCGTTTTG TGGCCATTAC CGGCGACGCC 
ATGGGCATGA ACATGGTGTC CAAGGGCGCT GAGATGGCCC TGCGCCGAAT TCAGCTTCAG 
TTTCCCGACA TGCAGATCAT CTCGCTGAGT GGAAACTTCT GCTGCGACAA GAAACCGGCG 
GCCATCAACT GGATTAAGGG ACGTGGCAAG CGGGTGGTCA CCGAGTGTAC CATTTCGGCA 
GCCACACTGC GTTCCGTGCT GAAGACAGAT GCCAAGACAC TGGTCGAATG TAACAAGCTG 
AAGAATATGG GAGGCAGCGC GATGGCCGGT AGCATTGGTG GCAACAATGC TCATGCCGCC 
AATATGGTGA CTGCCGTCTT TTTGGCCACG GGGCAGGATC CCGCCCAGAA TGTCACCTCG 
AGTAATTGCT CCACTGCCAT GGAGTGCTGG GCGGAGAACA GCGAGGATCT TTACATGACC 
TGCACAATGC CTTCGCTGGA GGTGGGAACT GTGGGCGGCG GAACCGGGTT GCCCGGCCAG 
AGCGCATGCC TGGAAATGCT CGGAGTCCGC GGAGCTCACG CCACGCGGCC AGGAGACAAC 
GCCAAGAAGC TGGCACAGAT CGTCTGCGCC ACGGTTATGG CCGGCGAATT GAGCCTGATG 
GCGGCGTTGG TCAATAGCGA TCTAGTCAAG AGTCATATGC GGCACAATCG ATCCTCCATC 
GCAGTGAACA GCGCCAACAA TCCTCTCAAC GTGACCGTGT CCAGCTGCAG CACCATCAGC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Hmgcr cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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