Plk1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Plk1
Entrez Gene ID	25515
Full Name	polo-like kinase 1
Synonyms	Plk
General protein information	Preferred Names polo-like kinase 1 Names serine/threonine-protein kinase PLK1 PLK-1 polo-like kinase homolog
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q36
Summary	human homolog catalyzes the phosphorylation of a Golgi reassembly stacking protein (GRASP65); may play a role in Golgi apparatus fragmentation and reorganization during mitosis [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_017100.1	NP_058796.1	serine/threonine-protein kinase PLK1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	plk1	NP_001003890.3
Caenorhabditis elegans (roundworm)	plk-2	NP_491036.1
Saccharomyces cerevisiae (baker's yeast)	CDC5	NP_013714.1
Drosophila melanogaster (fruit fly)	polo	NP_001014592.1
Kluyveromyces lactis	KLLA0C06138g	XP_452471.1
Eremothecium gossypii	AGOS_ACL006W	NP_983398.2
Pan troglodytes (chimpanzee)	PLK1	XP_001163623.2
Caenorhabditis elegans (roundworm)	plk-1	NP_001021173.1
Xenopus tropicalis (tropical clawed frog)	plk1	NP_998844.1
Homo sapiens (human)	PLK1	NP_005021.2
	PLK1	XP_001092070.1
Bos taurus (cattle)	PLK1	NP_001033262.1
Rattus norvegicus (Norway rat)	Plk1	NP_058796.1
Gallus gallus (chicken)	PLK1	NP_001025810.1
Canis lupus familiaris (dog)	PLK1	XP_547091.3
Mus musculus (house mouse)	Plk1	NP_035251.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004239	XP_313155.3

Gene Ontology
Bibliography

Related articles in PubMed

Sequential analysis of multistage hepatocarcinogenesis reveals that miR-100 and PLK1 dysregulation is an early event maintained along tumor progression.
Petrelli A, Perra A, Schernhuber K, Cargnelutti M, Salvi A, Migliore C, Ghiso E, Benetti A, Barlati S, Ledda-Columbano GM, Portolani N, De Petro G, Columbano A, Giordano S
Oncogene31(42)4517-26(2012 Oct)

Inhibition of Polo-like kinase 1 reduces beta-amyloid-induced neuronal cell death in Alzheimer's disease.
Song B, Davis K, Liu XS, Lee HG, Smith M, Liu X
Aging3(9)846-51(2011 Sep)

The peptidyl-prolyl isomerase Pin1 regulates cytokinesis through Cep55.
van der Horst A, Khanna KK
Cancer research69(16)6651-9(2009 Aug)

Over expression of Plk1 does not induce cell division in rat cardiac myocytes in vitro.
Coxon CH, Bicknell KA, Moseley FL, Brooks G
PloS one4(8)e6752(2009 Aug)

Ciprofloxacin-mediated cell proliferation inhibition and G2/M cell cycle arrest in rat tendon cells.
Tsai WC, Hsu CC, Tang FT, Wong AM, Chen YC, Pang JH
Arthritis and rheumatism58(6)1657-63(2008 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data indicate that miR-100 reduction was paralleled by an increased expression of polo like kinase 1 (PLK1).
Title: Sequential analysis of multistage hepatocarcinogenesis reveals that miR-100 and PLK1 dysregulation is an early event maintained along tumor progression.

Data show that Polo-like kinase 1 activity is elevated in Alzheimer's disease as indicated by the increased phosphorylation signal of p150Glued, a Plk1-specific substrate.
Title: Inhibition of Polo-like kinase 1 reduces beta-amyloid-induced neuronal cell death in Alzheimer's disease.

conclude that, in contrast to Cdc2/Cyclin B1 over-expression, the forced expression of Plk1 in adult cardiac myocytes is not sufficient to induce cell division and myocardial repair
Title: Over expression of Plk1 does not induce cell division in rat cardiac myocytes in vitro.

may be a multifunctional kinase that plays pivotal regulatory roles in microtubule assembly during rat oocyte meiotic maturation, fertilization, and early embryonic mitosis
Title: Characterization of Polo-like kinase-1 in rat oocytes and early embryos implies its functional roles in the regulation of meiotic maturation, fertilization, and cleavage.

The following Plk1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Plk1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa02833	NM_017100.1 Latest version!	Rattus norvegicus polo-like kinase 1 (Plk1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Plk1 ( NM_017100.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_058796.1 Rattus norvegicus polo-like kinase 1 (Plk1), mRNA.

Price & Availability↑

CloneID	ORa02833
Clone ID Related Accession (Same CDS sequence)	NM_017100.1
Accession Version	NM_017100.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1812bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-30
Organism	Rattus norvegicus(Norway rat)
Product	serine/threonine-protein kinase PLK1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from U10188.1. Sequence Note: This sequence has been modified as follows: removed 28 bp suspected to be vector contamination from the 3' end. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U10188.1, BC083926.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGAATGCAG CGGCCAAAGC TGGAAAGCTG GCTCGAGCAC CAGCCGACCT CGGGAAAGGT 
GGGGTTCCGG GAGATGCAGT TCCCGGTGCC CCAGTGGCCG CCCCGCTGGC GAAAGAAATT 
CCGGAGGTCC TAGTGGACCC ACGCAGCCGG CAGCAGTATG TACGGGGCCG CTTCCTGGGT 
AAAGGAGGCT TCGCCAAATG CTTCGAAATC TCAGACTCAG ACACAAAAGA GGTGTTCCCA 
GGCAAGATCG TGCCTAAGTC TTTGCTGCTC AAGCCCCATC AGAAGGAGAA AATGTCTATG 
GAGACCTCCA TTCACCGCAG CCTCGAACAC CAGCACGTCG TAGGCTTCCA TGGCTTTTTT 
GAGGACAGCG ACTTTGTGTT CGTGGTTTTG GAGCTCTGTC GCAGGAGGTC TCTCCTGGAG 
CTGCACAAAA GGAGGAAGGC GCTGACCGAG CCCGAGGCCC GATACTACCT GCGGCAGATA 
GTCCTGGGTT GCCAGTACCT GCACCGCAAT CAAGTCATTC ACAGGGACCT CAAACTGGGC 
AACCTCTTTC TGAATGAGGA TCTGGAGGTG AAAATAGGGG ATTTTGGCCT GGCAACAAAA 
GTGGAATATG AAGGGGAACG GAAGAAGACC CTGTGTGGCA CTCCTAACTA CATAGCTCCC 
GAGGTGCTGA GCAAGAAGGG ACACAGCTTT GAGGTGGACG TGTGGTCCAT TGGGTGCATC 
ATGTATACCT TGCTGGTGGG CAAGCCACCT TTTGAGACCT CATGCCTAAA AGAGACCTAC 
CTCCGGATCA AGAAAAATGA ATACAGTATT CCCAAGCACA TCAACCCAGT GGCTGCCTCC 
CTCATCCAGA AAATGCTTCA GACAGACCCC GCTGCGCGCC CCACCATTCA CGAGTTGCTC 
AATGACGAGT TCTTCACTTC CGGATACATC CCCGCCCGTC TGCCTATTAC CTGCCTCACC 
ATCCCACCAA GGTTTTCAAT AGCTCCCAGC AGCCTAGACC CCAGCAACAG GAAGCCTCTC 
ACAGTCCTTA ATAAAGGTGT GGAGAACCCC CTGCCTGACC GTCCCCGAGA GAAAGAGGAA 
CCAGTGGTTC GGGAGACAAA TGAGGCCATC GAGTGCCACC TTAGTGACTT GCTACAGCAG 
CTGACCAGTG TCAACGCCTC CAAGCCCTCA GAGCGAGGGC TGGTGCGGCA AGAGGAGGCC 
GAGGATCCTG CTTGCATCCC TATCTTCTGG GTCAGCAAAT GGGTAGACTA TTCGGACAAG 
TATGGCCTTG GTTATCAGCT ATGTGACAAC AGTGTGGGAG TGCTCTTTAA TGACTCAACA 
CGCCTCATCC TCTACAATGA CGGGGACAGC CTGCAGTACA TAGAGCGTGA TGGCACGGAG 
TCCTACCTCA CTGTGAGCTC CCACCCCAAC TCCTTGATGA AGAAGATCAC TCTCCTCAAC 
TATTTCCGCA ATTACATGAG TGAACACCTG CTGAAGGCGG GGGCCAACAT CACGCCCCGG 
GAAGGCGACG AGCTGGCCCG GCTGCCCTAC CTACGAACAT GGTTCCGCAC ACGCAGCGCC 
ATCATCCTGC ACCTCAGCAA TGGCACAGTA CAGATTAATT TCTTCCAGGA CCACACCAAA 
CTTATCCGTG GCCCCCTGAT GGCAGCAGTG ACCTACATCA ACGAGAAGAG GGACTTCCGG 
ACATACCGCC TGAGCCTTCT GGAGGAATAT GGCTGCTGCA AGGAACTGGC TAGCCGCCTC 
CGCTATGCCC GCACCATGGT AGACAAGCTG CTGAGCTCGC GTTCCGCCTG CAACCGCCTC 
AAGGCCTCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058796.1
CDS	95..1906
Translation	MNAAAKAGKLARAPADLGKGGVPGDAVPGAPVAAPLAKEIPEVLVDPRSRQQYVRGRFLGKGGFAKCFEISDSDTKEVFPGKIVPKSLLLKPHQKEKMSMETSIHRSLEHQHVVGFHGFFEDSDFVFVVLELCRRRSLLELHKRRKALTEPEARYYLRQIVLGCQYLHRNQVIHRDLKLGNLFLNEDLEVKIGDFGLATKVEYEGERKKTLCGTPNYIAPEVLSKKGHSFEVDVWSIGCIMYTLLVGKPPFETSCLKETYLRIKKNEYSIPKHINPVAASLIQKMLQTDPAARPTIHELLNDEFFTSGYIPARLPITCLTIPPRFSIAPSSLDPSNRKPLTVLNKGVENPLPDRPREKEEPVVRETNEAIECHLSDLLQQLTSVNASKPSERGLVRQEEAEDPACIPIFWVSKWVDYSDKYGLGYQLCDNSVGVLFNDSTRLILYNDGDSLQYIERDGTESYLTVSSHPNSLMKKITLLNYFRNYMSEHLLKAGANITPREGDELARLPYLRTWFRTRSAIILHLSNGTVQINFFQDHTKLIRGPLMAAVTYINEKRDFRTYRLSLLEEYGCCKELASRLRYARTMVDKLLSSRSACNRLKAS

Target ORF information:

RefSeq Version	NM_017100.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus polo-like kinase 1 (Plk1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017100.1

ORF Insert Sequence:

ATGAATGCAG CGGCCAAAGC TGGAAAGCTG GCTCGAGCAC CAGCCGACCT CGGGAAAGGT 
GGGGTTCCGG GAGATGCAGT TCCCGGTGCC CCAGTGGCCG CCCCGCTGGC GAAAGAAATT 
CCGGAGGTCC TAGTGGACCC ACGCAGCCGG CAGCAGTATG TACGGGGCCG CTTCCTGGGT 
AAAGGAGGCT TCGCCAAATG CTTCGAAATC TCAGACTCAG ACACAAAAGA GGTGTTCCCA 
GGCAAGATCG TGCCTAAGTC TTTGCTGCTC AAGCCCCATC AGAAGGAGAA AATGTCTATG 
GAGACCTCCA TTCACCGCAG CCTCGAACAC CAGCACGTCG TAGGCTTCCA TGGCTTTTTT 
GAGGACAGCG ACTTTGTGTT CGTGGTTTTG GAGCTCTGTC GCAGGAGGTC TCTCCTGGAG 
CTGCACAAAA GGAGGAAGGC GCTGACCGAG CCCGAGGCCC GATACTACCT GCGGCAGATA 
GTCCTGGGTT GCCAGTACCT GCACCGCAAT CAAGTCATTC ACAGGGACCT CAAACTGGGC 
AACCTCTTTC TGAATGAGGA TCTGGAGGTG AAAATAGGGG ATTTTGGCCT GGCAACAAAA 
GTGGAATATG AAGGGGAACG GAAGAAGACC CTGTGTGGCA CTCCTAACTA CATAGCTCCC 
GAGGTGCTGA GCAAGAAGGG ACACAGCTTT GAGGTGGACG TGTGGTCCAT TGGGTGCATC 
ATGTATACCT TGCTGGTGGG CAAGCCACCT TTTGAGACCT CATGCCTAAA AGAGACCTAC 
CTCCGGATCA AGAAAAATGA ATACAGTATT CCCAAGCACA TCAACCCAGT GGCTGCCTCC 
CTCATCCAGA AAATGCTTCA GACAGACCCC GCTGCGCGCC CCACCATTCA CGAGTTGCTC 
AATGACGAGT TCTTCACTTC CGGATACATC CCCGCCCGTC TGCCTATTAC CTGCCTCACC 
ATCCCACCAA GGTTTTCAAT AGCTCCCAGC AGCCTAGACC CCAGCAACAG GAAGCCTCTC 
ACAGTCCTTA ATAAAGGTGT GGAGAACCCC CTGCCTGACC GTCCCCGAGA GAAAGAGGAA 
CCAGTGGTTC GGGAGACAAA TGAGGCCATC GAGTGCCACC TTAGTGACTT GCTACAGCAG 
CTGACCAGTG TCAACGCCTC CAAGCCCTCA GAGCGAGGGC TGGTGCGGCA AGAGGAGGCC 
GAGGATCCTG CTTGCATCCC TATCTTCTGG GTCAGCAAAT GGGTAGACTA TTCGGACAAG 
TATGGCCTTG GTTATCAGCT ATGTGACAAC AGTGTGGGAG TGCTCTTTAA TGACTCAACA 
CGCCTCATCC TCTACAATGA CGGGGACAGC CTGCAGTACA TAGAGCGTGA TGGCACGGAG 
TCCTACCTCA CTGTGAGCTC CCACCCCAAC TCCTTGATGA AGAAGATCAC TCTCCTCAAC 
TATTTCCGCA ATTACATGAG TGAACACCTG CTGAAGGCGG GGGCCAACAT CACGCCCCGG 
GAAGGCGACG AGCTGGCCCG GCTGCCCTAC CTACGAACAT GGTTCCGCAC ACGCAGCGCC 
ATCATCCTGC ACCTCAGCAA TGGCACAGTA CAGATTAATT TCTTCCAGGA CCACACCAAA 
CTTATCCGTG GCCCCCTGAT GGCAGCAGTG ACCTACATCA ACGAGAAGAG GGACTTCCGG 
ACATACCGCC TGAGCCTTCT GGAGGAATAT GGCTGCTGCA AGGAACTGGC TAGCCGCCTC 
CGCTATGCCC GCACCATGGT AGACAAGCTG CTGAGCTCGC GTTCCGCCTG CAACCGCCTC 
AAGGCCTCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sequential analysis of multistage hepatocarcinogenesis reveals that miR-100 and PLK1 dysregulation is an early event maintained along tumor progression.
Oncogene31(42)4517-26(2012 Oct)
Petrelli A,Perra A,Schernhuber K,Cargnelutti M,Salvi A,Migliore C,Ghiso E,Benetti A,Barlati S,Ledda-Columbano GM,Portolani N,De Petro G,Columbano A,Giordano S

Inhibition of Polo-like kinase 1 reduces beta-amyloid-induced neuronal cell death in Alzheimer's disease.
Aging3(9)846-51(2011 Sep)
Song B,Davis K,Liu XS,Lee HG,Smith M,Liu X

The peptidyl-prolyl isomerase Pin1 regulates cytokinesis through Cep55.
Cancer research69(16)6651-9(2009 Aug)
van der Horst A,Khanna KK

Over expression of Plk1 does not induce cell division in rat cardiac myocytes in vitro.
PloS one4(8)e6752(2009 Aug)
Coxon CH,Bicknell KA,Moseley FL,Brooks G

Ciprofloxacin-mediated cell proliferation inhibition and G2/M cell cycle arrest in rat tendon cells.
Arthritis and rheumatism58(6)1657-63(2008 Jun)
Tsai WC,Hsu CC,Tang FT,Wong AM,Chen YC,Pang JH

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Plk1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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