polo cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	polo
Entrez Gene ID	40232
Full Name	CG12306 gene product from transcript CG12306-RA
Synonyms	0256/04,1324/08,CG12306,Dmel\CG12306,PLK1,POLO,POLO/PLK1,Polo,Polo kinase,anon-WO0172774.3,l(3)01673,l(3)77Aa,l(3)S025604,l(3)S132408
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 77B2-77B3\|3-46 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001014592.2	NP_001014592.1	polo, isoform B
NM_079455.4	NP_524179.2	polo, isoform A

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	plk1	NP_001003890.3
Caenorhabditis elegans (roundworm)	plk-2	NP_491036.1
Saccharomyces cerevisiae (baker's yeast)	CDC5	NP_013714.1
Drosophila melanogaster (fruit fly)	polo	NP_001014592.1
Kluyveromyces lactis	KLLA0C06138g	XP_452471.1
Eremothecium gossypii	AGOS_ACL006W	NP_983398.2
Pan troglodytes (chimpanzee)	PLK1	XP_001163623.2
Caenorhabditis elegans (roundworm)	plk-1	NP_001021173.1
Xenopus tropicalis (tropical clawed frog)	plk1	NP_998844.1
Homo sapiens (human)	PLK1	NP_005021.2
	PLK1	XP_001092070.1
Bos taurus (cattle)	PLK1	NP_001033262.1
Rattus norvegicus (Norway rat)	Plk1	NP_058796.1
Gallus gallus (chicken)	PLK1	NP_001025810.1
Canis lupus familiaris (dog)	PLK1	XP_547091.3
Mus musculus (house mouse)	Plk1	NP_035251.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004239	XP_313155.3

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Cdk1 phosphorylates the conserved centriole protein Sas-4 during mitosis. This creates a Polo-docking site that helps recruit Polo to daughter centrioles.
Title: Cdk1 Phosphorylates Drosophila Sas-4 to Recruit Polo to Daughter Centrioles and Convert Them to Centrosomes.

Both conditions impaired NSC lineage progression. Ca(2) mito homeostasis is influenced by Polo-mediated phosphorylation of a conserved residue in Miro
Title: Polo Kinase Phosphorylates Miro to Control ER-Mitochondria Contact Sites and Mitochondrial Ca(2+) Homeostasis in Neural Stem Cell Development.

Study shows that cnn exon1A-encoded proteins interact with Polo at 2 residues and that this interaction is required for polar body and pericentriolar matrix formation in syncytial embryos.
Title: The end of a monolith: Deconstructing the Cnn-Polo interaction.

Polo is an important in vivo regulator of the pathological functions of APP.
Title: Loss of Polo ameliorates APP-induced Alzheimer's disease-like symptoms in Drosophila.

During pericentriolar matrix formation exon 1A Cnn-Long Form proteins likely bind Polo kinase before phosphorylation by Polo for Cnn transport to the centrosome.
Title: An Amino-Terminal Polo Kinase Interaction Motif Acts in the Regulation of Centrosome Formation and Reveals a Novel Function for centrosomin (cnn) in Drosophila.

The following polo gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the polo cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa19089	NM_001014592.2 Latest version!	Drosophila melanogaster polo, transcript variant B (polo), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OFa19089	NM_079455.4 Latest version!	Drosophila melanogaster polo, transcript variant A (polo), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

polo ( NM_001014592.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001014592.1 Drosophila melanogaster polo, transcript variant B (polo), mRNA.

Price & Availability↑

CloneID	OFa19089
Clone ID Related Accession (Same CDS sequence)	NM_001014592.2 , NM_079455.4
Accession Version	NM_001014592.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1731bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	polo, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_001014592.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCGCGA AGCCCGAGGA TAAGAGCACG GATATTCCGG ATCGCCTCGT CGACATCAAC 
CAGCGGAAAA CCTACAAGCG CATGCGGTTC TTCGGCAAGG GCGGGTTTGC AAAATGTTAC 
GAGATCATCG ATGTGGAAAC CGACGACGTC TTCGCCGGCA AGATCGTATC CAAGAAGCTG 
ATGATCAAAC ACAATCAGAA GGAGAAGACC GCCCAGGAGA TAACTATTCA CCGCAGCCTT 
AACCATCCGA ACATTGTCAA GTTTCACAAC TACTTTGAAG ATTCGCAGAA TATCTACATT 
GTGCTGGAGC TGTGCAAGAA AAGATCCATG ATGGAGCTGC ACAAACGTAG GAAAAGCATT 
ACGGAGTTCG AATGCCGCTA CTACATTTAC CAGATAATCC AGGGCGTTAA GTACTTGCAC 
GATAACCGCA TTATCCATCG AGATCTGAAG CTGGGCAATC TCTTCCTCAA CGATTTGTTG 
CACGTGAAGA TCGGGGATTT CGGGTTGGCC ACGCGCATTG AGTATGAGGG CGAGCGAAAA 
AAGACCTTAT GCGGAACGCC CAACTATATA GCCCCGGAGA TCCTCACCAA GAAGGGCCAC 
TCCTTCGAGG TGGACATCTG GTCGATTGGC TGCGTCATGT ACACACTGCT TGTGGGCCAG 
CCGCCGTTCG AAACCAAGAC TCTGAAGGAT ACGTACTCGA AAATCAAGAA GTGCGAGTAC 
CGCGTGCCCA GCTACTTAAG GAAACCGGCG GCGGATATGG TCATCGCCAT GCTGCAGCCA 
AATCCGGAGA GCCGCCCGGC AATTGGTCAG CTGCTGAACT TTGAGTTCCT CAAGGGCTCA 
AAGGTGCCCA TGTTCTTGCC CAGCTCTTGT CTGACAATGG CGCCGCGTAT CGGCAGCAAC 
GACACCATCG AGGATTCGAT GCACCGCAAG CCACTGATGG AGATGAACGG CATCAGGCCC 
GACGACACTC GTCTGGAGTC GACCTTCCTC AAGGCCAATC TGCACGACGC CATTACCGCG 
TCAGCGCAGG TGTGCCGCCA CAGCGAGGAC TATCGCAGCG ATATCGAGAG CCTGTACCAG 
CAGCTCACTA ATCTTATCAA CGGAAAGCCG CGAATTCTGC AAGGCAATCT GGGCGACGAG 
AACACAGATC CTGCAGCGCA GCCGCTCTTC TGGATATCCA AGTGGGTTGA CTACAGCGAC 
AAGTACGGAT TTGGTTACCA GCTGTGCGAT GAGGGCATCG GCGTGATGTT CAACGACACC 
ACAAAGCTGA TCCTGCTGCC GAATCAGATC AACGTACACT TCATCGACAA GGATGGCAAG 
GAGACGTACA TGACCACCAC GGATTACTGC AAGTCGCTTG ACAAGAAGAT GAAGCTGCTG 
TCGTACTTTA AGCGCTACAT GATCGAGCAC CTGGTGAAGG CAGGTGCCAA CAATGTGAAC 
ATTGAGAGCG ATCAAATCTC GCGTATGCCC CATTTACACT CCTGGTTCCG TACAACATGT 
GCCGTAGTTA TGCATTTGAC CAACGGTTCT GTGCAGCTAA ACTTCTCAGA TCACATGAAG 
CTCATCCTCT GCCCGCGCAT GAGTGCTATA ACCTATATGG ACCAGGAGAA GAACTTCCGC 
ACCTACCGAT TTTCGACCAT TGTGGAGAAC GGCGTGTCTA AAGACTTGTA CCAGAAGATC 
CGATATGCCC AGGAGAAACT TAGGAAAATG CTGGAGAAGA TGTTCACATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001014592.1
CDS	264..1994
Translation	MAAKPEDKSTDIPDRLVDINQRKTYKRMRFFGKGGFAKCYEIIDVETDDVFAGKIVSKKLMIKHNQKEKTAQEITIHRSLNHPNIVKFHNYFEDSQNIYIVLELCKKRSMMELHKRRKSITEFECRYYIYQIIQGVKYLHDNRIIHRDLKLGNLFLNDLLHVKIGDFGLATRIEYEGERKKTLCGTPNYIAPEILTKKGHSFEVDIWSIGCVMYTLLVGQPPFETKTLKDTYSKIKKCEYRVPSYLRKPAADMVIAMLQPNPESRPAIGQLLNFEFLKGSKVPMFLPSSCLTMAPRIGSNDTIEDSMHRKPLMEMNGIRPDDTRLESTFLKANLHDAITASAQVCRHSEDYRSDIESLYQQLTNLINGKPRILQGNLGDENTDPAAQPLFWISKWVDYSDKYGFGYQLCDEGIGVMFNDTTKLILLPNQINVHFIDKDGKETYMTTTDYCKSLDKKMKLLSYFKRYMIEHLVKAGANNVNIESDQISRMPHLHSWFRTTCAVVMHLTNGSVQLNFSDHMKLILCPRMSAITYMDQEKNFRTYRFSTIVENGVSKDLYQKIRYAQEKLRKMLEKMFT

Target ORF information:

RefSeq Version	NM_001014592.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster polo, transcript variant B (polo), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001014592.2

ORF Insert Sequence:

ATGGCCGCGA AGCCCGAGGA TAAGAGCACG GATATTCCGG ATCGCCTCGT CGACATCAAC 
CAGCGGAAAA CCTACAAGCG CATGCGGTTC TTCGGCAAGG GCGGGTTTGC AAAATGTTAC 
GAGATCATCG ATGTGGAAAC CGACGACGTC TTCGCCGGCA AGATCGTATC CAAGAAGCTG 
ATGATCAAAC ACAATCAGAA GGAGAAGACC GCCCAGGAGA TAACTATTCA CCGCAGCCTT 
AACCATCCGA ACATTGTCAA GTTTCACAAC TACTTTGAAG ATTCGCAGAA TATCTACATT 
GTGCTGGAGC TGTGCAAGAA AAGATCCATG ATGGAGCTGC ACAAACGTAG GAAAAGCATT 
ACGGAGTTCG AATGCCGCTA CTACATTTAC CAGATAATCC AGGGCGTTAA GTACTTGCAC 
GATAACCGCA TTATCCATCG AGATCTGAAG CTGGGCAATC TCTTCCTCAA CGATTTGTTG 
CACGTGAAGA TCGGGGATTT CGGGTTGGCC ACGCGCATTG AGTATGAGGG CGAGCGAAAA 
AAGACCTTAT GCGGAACGCC CAACTATATA GCCCCGGAGA TCCTCACCAA GAAGGGCCAC 
TCCTTCGAGG TGGACATCTG GTCGATTGGC TGCGTCATGT ACACACTGCT TGTGGGCCAG 
CCGCCGTTCG AAACCAAGAC TCTGAAGGAT ACGTACTCGA AAATCAAGAA GTGCGAGTAC 
CGCGTGCCCA GCTACTTAAG GAAACCGGCG GCGGATATGG TCATCGCCAT GCTGCAGCCA 
AATCCGGAGA GCCGCCCGGC AATTGGTCAG CTGCTGAACT TTGAGTTCCT CAAGGGCTCA 
AAGGTGCCCA TGTTCTTGCC CAGCTCTTGT CTGACAATGG CGCCGCGTAT CGGCAGCAAC 
GACACCATCG AGGATTCGAT GCACCGCAAG CCACTGATGG AGATGAACGG CATCAGGCCC 
GACGACACTC GTCTGGAGTC GACCTTCCTC AAGGCCAATC TGCACGACGC CATTACCGCG 
TCAGCGCAGG TGTGCCGCCA CAGCGAGGAC TATCGCAGCG ATATCGAGAG CCTGTACCAG 
CAGCTCACTA ATCTTATCAA CGGAAAGCCG CGAATTCTGC AAGGCAATCT GGGCGACGAG 
AACACAGATC CTGCAGCGCA GCCGCTCTTC TGGATATCCA AGTGGGTTGA CTACAGCGAC 
AAGTACGGAT TTGGTTACCA GCTGTGCGAT GAGGGCATCG GCGTGATGTT CAACGACACC 
ACAAAGCTGA TCCTGCTGCC GAATCAGATC AACGTACACT TCATCGACAA GGATGGCAAG 
GAGACGTACA TGACCACCAC GGATTACTGC AAGTCGCTTG ACAAGAAGAT GAAGCTGCTG 
TCGTACTTTA AGCGCTACAT GATCGAGCAC CTGGTGAAGG CAGGTGCCAA CAATGTGAAC 
ATTGAGAGCG ATCAAATCTC GCGTATGCCC CATTTACACT CCTGGTTCCG TACAACATGT 
GCCGTAGTTA TGCATTTGAC CAACGGTTCT GTGCAGCTAA ACTTCTCAGA TCACATGAAG 
CTCATCCTCT GCCCGCGCAT GAGTGCTATA ACCTATATGG ACCAGGAGAA GAACTTCCGC 
ACCTACCGAT TTTCGACCAT TGTGGAGAAC GGCGTGTCTA AAGACTTGTA CCAGAAGATC 
CGATATGCCC AGGAGAAACT TAGGAAAATG CTGGAGAAGA TGTTCACATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

polo ( NM_079455.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524179.2 Drosophila melanogaster polo, transcript variant A (polo), mRNA.

Price & Availability↑

CloneID	OFa19089
Clone ID Related Accession (Same CDS sequence)	NM_001014592.2 , NM_079455.4
Accession Version	NM_079455.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1731bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	polo, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_079455.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGCCGCGA AGCCCGAGGA TAAGAGCACG GATATTCCGG ATCGCCTCGT CGACATCAAC 
CAGCGGAAAA CCTACAAGCG CATGCGGTTC TTCGGCAAGG GCGGGTTTGC AAAATGTTAC 
GAGATCATCG ATGTGGAAAC CGACGACGTC TTCGCCGGCA AGATCGTATC CAAGAAGCTG 
ATGATCAAAC ACAATCAGAA GGAGAAGACC GCCCAGGAGA TAACTATTCA CCGCAGCCTT 
AACCATCCGA ACATTGTCAA GTTTCACAAC TACTTTGAAG ATTCGCAGAA TATCTACATT 
GTGCTGGAGC TGTGCAAGAA AAGATCCATG ATGGAGCTGC ACAAACGTAG GAAAAGCATT 
ACGGAGTTCG AATGCCGCTA CTACATTTAC CAGATAATCC AGGGCGTTAA GTACTTGCAC 
GATAACCGCA TTATCCATCG AGATCTGAAG CTGGGCAATC TCTTCCTCAA CGATTTGTTG 
CACGTGAAGA TCGGGGATTT CGGGTTGGCC ACGCGCATTG AGTATGAGGG CGAGCGAAAA 
AAGACCTTAT GCGGAACGCC CAACTATATA GCCCCGGAGA TCCTCACCAA GAAGGGCCAC 
TCCTTCGAGG TGGACATCTG GTCGATTGGC TGCGTCATGT ACACACTGCT TGTGGGCCAG 
CCGCCGTTCG AAACCAAGAC TCTGAAGGAT ACGTACTCGA AAATCAAGAA GTGCGAGTAC 
CGCGTGCCCA GCTACTTAAG GAAACCGGCG GCGGATATGG TCATCGCCAT GCTGCAGCCA 
AATCCGGAGA GCCGCCCGGC AATTGGTCAG CTGCTGAACT TTGAGTTCCT CAAGGGCTCA 
AAGGTGCCCA TGTTCTTGCC CAGCTCTTGT CTGACAATGG CGCCGCGTAT CGGCAGCAAC 
GACACCATCG AGGATTCGAT GCACCGCAAG CCACTGATGG AGATGAACGG CATCAGGCCC 
GACGACACTC GTCTGGAGTC GACCTTCCTC AAGGCCAATC TGCACGACGC CATTACCGCG 
TCAGCGCAGG TGTGCCGCCA CAGCGAGGAC TATCGCAGCG ATATCGAGAG CCTGTACCAG 
CAGCTCACTA ATCTTATCAA CGGAAAGCCG CGAATTCTGC AAGGCAATCT GGGCGACGAG 
AACACAGATC CTGCAGCGCA GCCGCTCTTC TGGATATCCA AGTGGGTTGA CTACAGCGAC 
AAGTACGGAT TTGGTTACCA GCTGTGCGAT GAGGGCATCG GCGTGATGTT CAACGACACC 
ACAAAGCTGA TCCTGCTGCC GAATCAGATC AACGTACACT TCATCGACAA GGATGGCAAG 
GAGACGTACA TGACCACCAC GGATTACTGC AAGTCGCTTG ACAAGAAGAT GAAGCTGCTG 
TCGTACTTTA AGCGCTACAT GATCGAGCAC CTGGTGAAGG CAGGTGCCAA CAATGTGAAC 
ATTGAGAGCG ATCAAATCTC GCGTATGCCC CATTTACACT CCTGGTTCCG TACAACATGT 
GCCGTAGTTA TGCATTTGAC CAACGGTTCT GTGCAGCTAA ACTTCTCAGA TCACATGAAG 
CTCATCCTCT GCCCGCGCAT GAGTGCTATA ACCTATATGG ACCAGGAGAA GAACTTCCGC 
ACCTACCGAT TTTCGACCAT TGTGGAGAAC GGCGTGTCTA AAGACTTGTA CCAGAAGATC 
CGATATGCCC AGGAGAAACT TAGGAAAATG CTGGAGAAGA TGTTCACATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524179.2
CDS	657..2387
Translation	MAAKPEDKSTDIPDRLVDINQRKTYKRMRFFGKGGFAKCYEIIDVETDDVFAGKIVSKKLMIKHNQKEKTAQEITIHRSLNHPNIVKFHNYFEDSQNIYIVLELCKKRSMMELHKRRKSITEFECRYYIYQIIQGVKYLHDNRIIHRDLKLGNLFLNDLLHVKIGDFGLATRIEYEGERKKTLCGTPNYIAPEILTKKGHSFEVDIWSIGCVMYTLLVGQPPFETKTLKDTYSKIKKCEYRVPSYLRKPAADMVIAMLQPNPESRPAIGQLLNFEFLKGSKVPMFLPSSCLTMAPRIGSNDTIEDSMHRKPLMEMNGIRPDDTRLESTFLKANLHDAITASAQVCRHSEDYRSDIESLYQQLTNLINGKPRILQGNLGDENTDPAAQPLFWISKWVDYSDKYGFGYQLCDEGIGVMFNDTTKLILLPNQINVHFIDKDGKETYMTTTDYCKSLDKKMKLLSYFKRYMIEHLVKAGANNVNIESDQISRMPHLHSWFRTTCAVVMHLTNGSVQLNFSDHMKLILCPRMSAITYMDQEKNFRTYRFSTIVENGVSKDLYQKIRYAQEKLRKMLEKMFT

Target ORF information:

RefSeq Version	NM_079455.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster polo, transcript variant A (polo), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079455.4

ORF Insert Sequence:

ATGGCCGCGA AGCCCGAGGA TAAGAGCACG GATATTCCGG ATCGCCTCGT CGACATCAAC 
CAGCGGAAAA CCTACAAGCG CATGCGGTTC TTCGGCAAGG GCGGGTTTGC AAAATGTTAC 
GAGATCATCG ATGTGGAAAC CGACGACGTC TTCGCCGGCA AGATCGTATC CAAGAAGCTG 
ATGATCAAAC ACAATCAGAA GGAGAAGACC GCCCAGGAGA TAACTATTCA CCGCAGCCTT 
AACCATCCGA ACATTGTCAA GTTTCACAAC TACTTTGAAG ATTCGCAGAA TATCTACATT 
GTGCTGGAGC TGTGCAAGAA AAGATCCATG ATGGAGCTGC ACAAACGTAG GAAAAGCATT 
ACGGAGTTCG AATGCCGCTA CTACATTTAC CAGATAATCC AGGGCGTTAA GTACTTGCAC 
GATAACCGCA TTATCCATCG AGATCTGAAG CTGGGCAATC TCTTCCTCAA CGATTTGTTG 
CACGTGAAGA TCGGGGATTT CGGGTTGGCC ACGCGCATTG AGTATGAGGG CGAGCGAAAA 
AAGACCTTAT GCGGAACGCC CAACTATATA GCCCCGGAGA TCCTCACCAA GAAGGGCCAC 
TCCTTCGAGG TGGACATCTG GTCGATTGGC TGCGTCATGT ACACACTGCT TGTGGGCCAG 
CCGCCGTTCG AAACCAAGAC TCTGAAGGAT ACGTACTCGA AAATCAAGAA GTGCGAGTAC 
CGCGTGCCCA GCTACTTAAG GAAACCGGCG GCGGATATGG TCATCGCCAT GCTGCAGCCA 
AATCCGGAGA GCCGCCCGGC AATTGGTCAG CTGCTGAACT TTGAGTTCCT CAAGGGCTCA 
AAGGTGCCCA TGTTCTTGCC CAGCTCTTGT CTGACAATGG CGCCGCGTAT CGGCAGCAAC 
GACACCATCG AGGATTCGAT GCACCGCAAG CCACTGATGG AGATGAACGG CATCAGGCCC 
GACGACACTC GTCTGGAGTC GACCTTCCTC AAGGCCAATC TGCACGACGC CATTACCGCG 
TCAGCGCAGG TGTGCCGCCA CAGCGAGGAC TATCGCAGCG ATATCGAGAG CCTGTACCAG 
CAGCTCACTA ATCTTATCAA CGGAAAGCCG CGAATTCTGC AAGGCAATCT GGGCGACGAG 
AACACAGATC CTGCAGCGCA GCCGCTCTTC TGGATATCCA AGTGGGTTGA CTACAGCGAC 
AAGTACGGAT TTGGTTACCA GCTGTGCGAT GAGGGCATCG GCGTGATGTT CAACGACACC 
ACAAAGCTGA TCCTGCTGCC GAATCAGATC AACGTACACT TCATCGACAA GGATGGCAAG 
GAGACGTACA TGACCACCAC GGATTACTGC AAGTCGCTTG ACAAGAAGAT GAAGCTGCTG 
TCGTACTTTA AGCGCTACAT GATCGAGCAC CTGGTGAAGG CAGGTGCCAA CAATGTGAAC 
ATTGAGAGCG ATCAAATCTC GCGTATGCCC CATTTACACT CCTGGTTCCG TACAACATGT 
GCCGTAGTTA TGCATTTGAC CAACGGTTCT GTGCAGCTAA ACTTCTCAGA TCACATGAAG 
CTCATCCTCT GCCCGCGCAT GAGTGCTATA ACCTATATGG ACCAGGAGAA GAACTTCCGC 
ACCTACCGAT TTTCGACCAT TGTGGAGAAC GGCGTGTCTA AAGACTTGTA CCAGAAGATC 
CGATATGCCC AGGAGAAACT TAGGAAAATG CTGGAGAAGA TGTTCACATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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polo cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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