gRNAs
The following Nes gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nes cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | ORa24845 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001308239.1 , XM_017590648.1 | |
| Accession Version | NM_001308239.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 5682bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-12-29 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | nestin isoform 1 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07072748.1. On Apr 28, 2015 this sequence version replaced XM_006232600.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF538924.1, CB764084.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMEA2689595 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGGAGGGTT GCGTCGGGGA AGAATCTTTT CAGATGTGGG AGCTCAATCG ACGCCTGGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001295168.1 |
| CDS | 128..5809 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_001308239.1 |
1 | ATGGAGGGTT GCGTCGGGGA AGAATCTTTT CAGATGTGGG AGCTCAATCG ACGCCTGGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ORa43124 | |
| Clone ID Related Accession (Same CDS sequence) | NM_012987.2 | |
| Accession Version | NM_012987.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 5418bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-12-28 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | nestin isoform 2 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07072748.1. On Apr 28, 2015 this sequence version replaced NM_012987.1. Transcript Variant: This variant (2) lacks an alternate in-frame segment compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M34384.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMEA2689595 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGGAGGGTT GCGTCGGGGA AGAATCTTTT CAGATGTGGG AGCTCAATCG ACGCCTGGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_037119.2 |
| CDS | 128..5545 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_012987.2 |
1 | ATGGAGGGTT GCGTCGGGGA AGAATCTTTT CAGATGTGGG AGCTCAATCG ACGCCTGGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ORa24845 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001308239.1 , XM_017590648.1 | |
| Accession Version | XM_017590648.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 5682bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-07-25 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | nestin isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGAGGGTT GCGTCGGGGA AGAATCTTTT CAGATGTGGG AGCTCAATCG ACGCCTGGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_017446137.1 |
| CDS | 542..6223 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_017590648.1 |
1 | ATGGAGGGTT GCGTCGGGGA AGAATCTTTT CAGATGTGGG AGCTCAATCG ACGCCTGGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Nestin Expression and In Vivo Proliferative Potential of Tanycytes and Ependymal Cells Lining the Walls of the Third Ventricle in the Adult Rat Brain. |
Nestin Expressed by Pre-Existing Cardiomyocytes Recapitulated in Part an Embryonic Phenotype; Suppressive Role of p38 MAPK. |
Nestin expression is upregulated in the fibrotic rat heart and is localized in collagen-expressing mesenchymal cells and interstitial CD31(+)- cells. |
Genetically engineered rat gliomas: PDGF-driven tumor initiation and progression in tv-a transgenic rats recreate key features of human brain cancer. |
