NES cDNA ORF clone, Bos taurus(cattle)

The following NES gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NES cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa232777 XM_024989295.1
Latest version!
Bos taurus nestin (NES), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa232778 XM_024989303.1
Latest version!
Bos taurus nestin (NES), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa232779 XM_024989307.1
Latest version!
Bos taurus nestin (NES), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa37137 NM_001206591.1
Latest version!
Bos taurus nestin (NES), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa232777
    Clone ID Related Accession (Same CDS sequence) XM_024989295.1
    Accession Version XM_024989295.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5337bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product nestin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGAGGGCT GCCTGGGGGA GGAATCTTTT CAGATGTGGG AGCTCAACCG GCGCCTGGAG 
    GCCTACCTGG CCCGGGTCAA GGCGCTAGAA GAGCAGAATG AGCTGCTCAG CGCGGAGCTC
    GGGGGTCTCC GGGCACAGGC CGGGGACACC TCCTGGAGGG CTCGTGCCGA CGACGAGCTG
    GCGGCCCTAC GGGCCCTCGT CGACCAGCGC TGGCGGGAGA AGCACGCGGC CGAGGTGGCG
    CGCGACAACC TGGCAGAAGA GGTGGAGGGC GTGGCGAGTC GGTGCCAGCA GCTGCGGCTG
    GCCCGGGAGC GGACCGCGGA GGAGGTGGCC CGCAGCCGGC GTGCGGTCGA GGCCGAGAAA
    TGCGCCCAGG CCTGGCTGAG CACCCAGGCT GCGGAGCTGG AGCGCGAGCT GGAGGCTCTG
    CGCGTGGCGC AAGAGGAGGA GCGCGCCGGC CTGAACGCGC AGGCTGCCTG CGCCCCCCGC
    GGCTCCGCTC CGCCCCGGGG TCCCCCCGCG CCAGCCCCGG AGGTGGAGGA GCTGGCTCAG
    CGGCTGGGCG AGGCGTGGCG TGGGGCAGTG CGCGGCTACC AGGAACGCGT GGCGCACATG
    GAGACGTCGC TGGGCCAGGC CCGCGAGCGG CTAGGCCACG CGATGCAGGG CGCCCGCGAG
    GGTCGCCTGG AGCTGCAGCA ACTCCAGGCA GAGCGCAGCG GCCTTCAGGA GCGCAGGGCC
    GCGCTGGAGC AGAGGCTGGA GGGCCGCTGG CAGGAGCGGC TGCGGACCAC TGAGCAGTTC
    CAGCTGGCCG TGGAGGCCCT GGAGCAGGAG AAACAAGGCC TCCAGAGCCA AATCGCCCAG
    GTCCTGGAAG GTCGGCAGCA GCTGGCACAC CTCAAGATGT CCCTCAGCCT GGAGGTGGCC
    ACATACAGGA CCCTCCTAGA GGCTGAGAAC TCACGGCTGC AGACACCTGC AAGCGGTTCC
    AAGGCTTCCC TCAGCTTTCT GGACCCTAAG CTGGAGCTAC ATTTCCCTGG GACCCCAGAG
    GGCCGGCGTC CGGGACCTCT GCTTTCTGTC CTGAGCCCTA CTCCCCTCTC CTCACCTTTG
    CCTGGTACCC TTGAAACACC TGTGCCAACC TTTCTGAAGA GCCAGGAATT CCTTCAGGCT
    CGCACCCCGA CCTCAGCCAG CACCCCCATC CCACCCACCC CTCAGGCTCC CTGCCCTGCT
    GTAGATGCCG AGATCAGAGC CCAGGATGCC CCTGTGTCTC TGCCCCAAGC ACGAGTTGGG
    AGGCAACAGG TGCCAGAAGT CATGTGGGCT GAAGCCAAGG TGGCCATCCC CGCCAGCGTC
    CTGCCAGGAC CAGAGGAGCC TGGGGGCCAG CAACAAGAGC CCAGTCCAAG CCAGTCCCCT
    GAAGATCATG CCTCCCTGGC TCCAGCCCTC AGCCCTGACC ACTCCAGTCT AGAGACCAAA
    GATGGAGAAC CCAGTGCATC TAGAGAGTCC AGCAGATCCC AGGAGGAAGA TGAAGGCCAA
    CTGTGGGGGC TGACAGAGAA AGAAACAGTG GTAGAGGCCA AAGCAGTGAG CAGCTTGCAG
    CAGGAAACGT GTCAAGAAGA GGCGGGTCTG GACGTGAAAG AAATCCAAGA TGCCCAGGGC
    CCTTTGGAAA AAGAAGCTCT GAACTCTCTG GAAGAAGAGA TTCAAAAGCC ATCGATTCCA
    CTGGAAAAAC AGAGCCATGA GACTATAAGA TCTCTAGAGA AGGAGAATCT GGAATCTCTG
    AGGTCTTTGG AAGAAGAGAA CTTAGAAACA CTAAAAATAC TAGAAAAGGA GAATCAAGAA
    TTATTGAAGT CTTTAGAAGG AAAGGAAACG GAGGTAATGA GATCTCTAGA AAAAGAAACT
    CTAGAACTAC TTAAGCCTAT AGGAAAAGAG GATCCACAGA CATTGCCATC TCTAGGAAAG
    GAGAATCAAG AAATAATGAG GTCTCTTGAA GTTAATGTAG AGACATTCTT ATATCCAGGA
    AAGGAAAATC AAGAATTAGT GAGGTCTTTA GAAGAGGAGA ACGCTGAGTC ATTGAGAACT
    CTAGAAAAGG AGAGTCAAGA GCCACTGAGA TGTCAAGAAG TAGAGAACCA GGGAACACTG
    AGACTCCTAG CCAAAGACAA TCAGGAGCCA CTGAGGTCTC TAGAAGAAGA AGACCAGGAG
    ACGTCAAGAC CGCTAGGGAA AGAGAATCAT GAATCCCTGC GGGCTCCAGA AGATGAGAAT
    CATGAGGCTT TGAGACCTCT AGAAAGAGAC AAACCAGAGT CCCTGAGTTC TTTAGAAGAA
    GACCAGGAGG CAGTGAGACC TCCAGAAAGA GAAGACCAGG AGCTGCTGAG GTCTCTAGAA
    AAAGAAGACC AGGAGCCACT GAGGTCTCTA GAAGAAGACC AGGAGGCAGT GAGACCTCTA
    GAAGAAGAAA ACCAGGAGCT GCTGAGGTCT CTAGAAAAAG AAAAAGAGGA GTCACTGAGG
    TCTCTAGAAG ACCAGGAGGC AATGAGACCT CTAGAAGAAG AAAACCAGGA GCCGCTGAGG
    TCTCTAGAAA AAGAAAAACA GGAGGCGACG AGACCTCCAG AAGAAGAAAA ACAGGAGCCA
    CTGATGTCTC TAGAAGAAGA CCAGGAGGAA ATGAGAGCTT TAGAAGAAGA CCAGGAGACA
    GTGAGACCTC TAGAAAAAGA AAAACAGGAG TCACTAAGGT CTCTAGAAGA CCACGAGGCA
    ATGAGACCTC TAGAAGAAGA AAACCAGGAG CCGCTGAGGT CTCTAGAAAA AGAAAAACAG
    GAGGTGATGA GACCTCCAGA AGAAGAAAAA CAGGAATTAC TGAGATCTCT AGAAGACCAG
    GAGGCAATGA GATCTCTAGA AGAAGAAGGC CAGATGACAT TGAGCCCTCT AGAAAAAGTG
    AAACCAGAGA CACTAAAGTC TCTTGGAAAA GATCAGGAGA TAGTTAGACC TCTTGAAAAA
    GAGAATCAAG AGTTATTGAG GTCCCTAAAT GAAAAGAGTA CAGAGGCAGT GAGGTCTTTA
    GAAACAGAGA CTCCAGAACC ACTAAAGCCT ACAGAAGAGG AAAACCTGGA AATACTGAAA
    CCTCTAGAAA AGGAAAGTCA AGAGCCACTG GGGTCTGTGG AAGGGAACCA TGAGACACTG
    AGACCCCCAG AGAAGGAGAA TCAAGAGTCA CTGGGCTCTC TGGCAGAGTG GAACGTGGAG
    AATTTGCAGT CTCTAGAGGA GGCCGACAAG GGAAGTCTAA GGCACTTGGA AGAGGAAGAG
    GACGTGGAGC AGGAAGAGAG TCAGGCGTCA CTGAGGCCCC TGGAGGAGCA GGGGCAGGAG
    CTGCCACTCT CTGCACAGCA GCAGAAGTGG GGAGATGCAA TGCAGGGGGA CCAAGAACTG
    GATCAGGAGC GGCCTCCGGG GAGGGCTGGA GTGGACAGTG GGGATGGGGC AGAGCTGGAA
    CTGAAAGAAC TGGATAGCTC CCCTGCGGAG GGGGAGGTGG TGGAGCAGGG TCAGCTGCAG
    CTGACAGCCA CAGGTGAGGC CTGGGGCCCA GGTGAGGGGC AGCCAGGCAG CCCTGAGCCC
    AAAGAGCAGA GGCTCCCAGC TGAGGGTGCC GGAGGGGCAG GAGGCGCTGC GGGCCTCCAG
    GACCCTGAGG AGCAGCCAGA GCAGGTGGGG GCCCTGGGCC TCCCGGCTGC CCAGGGAACA
    TCAGAGGTGA TGGAGCCCGA GTTGGAAGAT GAGGATGTGG CCCCAGGGGG TGGCCGAGCC
    TCCCCAGAGG TCACCTTGGA GTTAGAGAGG GCCATGGGCA GGTCTGAGGG AGTGGAACGG
    GGACCCGAGC AGGAGGCAGT AAAGCTGGAG GACCCAGGTG GCCTGGCCAG AGAGGAGGTG
    ATGGAGCCAC CCCTGGGGGA GGGAGGTATG GCAGCAAAGA AGGTACAGGG CTTGGAAGGG
    CCCAGAAAGG AGCTGGAGGA GGCAGGCACT CTGGAGCCTG AGTCCGGGAA GAGCAGAGAC
    CCGTTGGAGA CTCCTAGGGA CTGGGAGGAG TCAGAATCTG GGGCCCTTGG GAAAACAGAG
    GAGCCAGTCT CAGCTGAGAC CTTGTGGTCT CACGAGGGAA GTGATACCCT CCAGCCCAGG
    CCCCTGGGGT CAGAGGGAGC TGAGGAGGAT GCCAAACCAC TGCTGGGGCC CCCCAGTCTG
    AGGCCCACTG AGTCCTGCTT GCCCAGCCTA ATCCCTGAAG ATGCCCCTGG GCCCCAGCCC
    CTGGCTGAGG GGAACCAAGA AGCCAGCTGG GGGCTGGAGG GCAGGGCTGA GGTCGTAGGA
    AAGGCAGGAG GTGAGCAGGA GGATCTGGGC TCTGGGGGGA TCCCTGAGGG CCTCCAGGAG
    GAAGGGGAGG AGAGCAGAGA AGAGAGCGAG GCGGATGAGC TAGGGGAGAC CCTTCCTGAC
    TCCACTCCCC TAGGCCTCTA CCTCAGGTCC CCCGCTTCCC CCAAGTGGGA CCTGCCTGGA
    GAGCAGAGGC CCTCCCCTCA AGGGGAGCCC AGAAAAGAAG GCTGGGATCC TGTGGTCCCA
    GCCTCCAAGA GCCTTGGGGC CCAGCCCTCA GAGGAGGAAG AAGAGGAGGG AGCTGAGGAG
    GAGGAACGTG GCCATGATTC TGAGCTGTCG GAAGAGTTTG AGGACCTGGG GACTGAGGCT
    TCTCTCCTTC CCGGGGTCCC GGGGGAGGGG GAAGAACCTC TGAGCCAAGT GCCCCAGCTG
    CTCCTGGAGC CTGCAGCCTG GGATCGTGAT GGTGAATCTG ACGGATTTGC AGATGAGGAA
    GAGAGCGGGG AGGAGGGCGA GGAAGAAGAT GAAGAAGAGG GGAGGGAGCC AGGGCCAGGG
    CATTGGGGGC CAGCGCCCTC TGTGGGCAGC CTCCCCGCCC TGAGCGGCCC TCAGGGAGGG
    CACCTCCTGG AGTCTGAGAC CGTGGGTGTC AGTGTCCCCT GGGACGATGG CCCAAGGGGC
    GCGGCAGCCG ACGCCCCCGT GACTGCCCTG GAGACCGAGT CCCAGAACAG CACTGAGGCC
    TCAGGCTCAG AGGAAGAGTC TGATGCTGCT CCCCTGGAGA CGGAGGACCA GGTCCCTGGC
    CCTCTGGGGA CCCTCAGCGG GGTGGAGGAC ACCCTTGATG GTGGCCCAGG CCCCAGCCTG
    AAGGAAGAGC TGGAGCGCAT GAATGGGGGC GTGGTGAACG GGCTGGAGCA GTCCGAGGGG
    GGAGGCCAGG GGAAACCGGG GGCTGCTGGG GGGGACCGAG GGAGCCCCTC AGAGGAGGAG
    GGGGCTTCCC TGAAGGCCCC ATGGGCAGGG GCTCCTCTTC ACCTGGGCCA AGGCCAGTTC
    CTGAAGTTCA GTCAGAGAGA AGGTGATGGA GACTCCTGGT CCTCAGGGGA GGACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024845063.1
    CDS162..5498
    Translation

    Target ORF information:

    RefSeq Version XM_024989295.1
    Organism Bos taurus(cattle)
    Definition Bos taurus nestin (NES), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024989295.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGGAGGGCT GCCTGGGGGA GGAATCTTTT CAGATGTGGG AGCTCAACCG GCGCCTGGAG 
    GCCTACCTGG CCCGGGTCAA GGCGCTAGAA GAGCAGAATG AGCTGCTCAG CGCGGAGCTC
    GGGGGTCTCC GGGCACAGGC CGGGGACACC TCCTGGAGGG CTCGTGCCGA CGACGAGCTG
    GCGGCCCTAC GGGCCCTCGT CGACCAGCGC TGGCGGGAGA AGCACGCGGC CGAGGTGGCG
    CGCGACAACC TGGCAGAAGA GGTGGAGGGC GTGGCGAGTC GGTGCCAGCA GCTGCGGCTG
    GCCCGGGAGC GGACCGCGGA GGAGGTGGCC CGCAGCCGGC GTGCGGTCGA GGCCGAGAAA
    TGCGCCCAGG CCTGGCTGAG CACCCAGGCT GCGGAGCTGG AGCGCGAGCT GGAGGCTCTG
    CGCGTGGCGC AAGAGGAGGA GCGCGCCGGC CTGAACGCGC AGGCTGCCTG CGCCCCCCGC
    GGCTCCGCTC CGCCCCGGGG TCCCCCCGCG CCAGCCCCGG AGGTGGAGGA GCTGGCTCAG
    CGGCTGGGCG AGGCGTGGCG TGGGGCAGTG CGCGGCTACC AGGAACGCGT GGCGCACATG
    GAGACGTCGC TGGGCCAGGC CCGCGAGCGG CTAGGCCACG CGATGCAGGG CGCCCGCGAG
    GGTCGCCTGG AGCTGCAGCA ACTCCAGGCA GAGCGCAGCG GCCTTCAGGA GCGCAGGGCC
    GCGCTGGAGC AGAGGCTGGA GGGCCGCTGG CAGGAGCGGC TGCGGACCAC TGAGCAGTTC
    CAGCTGGCCG TGGAGGCCCT GGAGCAGGAG AAACAAGGCC TCCAGAGCCA AATCGCCCAG
    GTCCTGGAAG GTCGGCAGCA GCTGGCACAC CTCAAGATGT CCCTCAGCCT GGAGGTGGCC
    ACATACAGGA CCCTCCTAGA GGCTGAGAAC TCACGGCTGC AGACACCTGC AAGCGGTTCC
    AAGGCTTCCC TCAGCTTTCT GGACCCTAAG CTGGAGCTAC ATTTCCCTGG GACCCCAGAG
    GGCCGGCGTC CGGGACCTCT GCTTTCTGTC CTGAGCCCTA CTCCCCTCTC CTCACCTTTG
    CCTGGTACCC TTGAAACACC TGTGCCAACC TTTCTGAAGA GCCAGGAATT CCTTCAGGCT
    CGCACCCCGA CCTCAGCCAG CACCCCCATC CCACCCACCC CTCAGGCTCC CTGCCCTGCT
    GTAGATGCCG AGATCAGAGC CCAGGATGCC CCTGTGTCTC TGCCCCAAGC ACGAGTTGGG
    AGGCAACAGG TGCCAGAAGT CATGTGGGCT GAAGCCAAGG TGGCCATCCC CGCCAGCGTC
    CTGCCAGGAC CAGAGGAGCC TGGGGGCCAG CAACAAGAGC CCAGTCCAAG CCAGTCCCCT
    GAAGATCATG CCTCCCTGGC TCCAGCCCTC AGCCCTGACC ACTCCAGTCT AGAGACCAAA
    GATGGAGAAC CCAGTGCATC TAGAGAGTCC AGCAGATCCC AGGAGGAAGA TGAAGGCCAA
    CTGTGGGGGC TGACAGAGAA AGAAACAGTG GTAGAGGCCA AAGCAGTGAG CAGCTTGCAG
    CAGGAAACGT GTCAAGAAGA GGCGGGTCTG GACGTGAAAG AAATCCAAGA TGCCCAGGGC
    CCTTTGGAAA AAGAAGCTCT GAACTCTCTG GAAGAAGAGA TTCAAAAGCC ATCGATTCCA
    CTGGAAAAAC AGAGCCATGA GACTATAAGA TCTCTAGAGA AGGAGAATCT GGAATCTCTG
    AGGTCTTTGG AAGAAGAGAA CTTAGAAACA CTAAAAATAC TAGAAAAGGA GAATCAAGAA
    TTATTGAAGT CTTTAGAAGG AAAGGAAACG GAGGTAATGA GATCTCTAGA AAAAGAAACT
    CTAGAACTAC TTAAGCCTAT AGGAAAAGAG GATCCACAGA CATTGCCATC TCTAGGAAAG
    GAGAATCAAG AAATAATGAG GTCTCTTGAA GTTAATGTAG AGACATTCTT ATATCCAGGA
    AAGGAAAATC AAGAATTAGT GAGGTCTTTA GAAGAGGAGA ACGCTGAGTC ATTGAGAACT
    CTAGAAAAGG AGAGTCAAGA GCCACTGAGA TGTCAAGAAG TAGAGAACCA GGGAACACTG
    AGACTCCTAG CCAAAGACAA TCAGGAGCCA CTGAGGTCTC TAGAAGAAGA AGACCAGGAG
    ACGTCAAGAC CGCTAGGGAA AGAGAATCAT GAATCCCTGC GGGCTCCAGA AGATGAGAAT
    CATGAGGCTT TGAGACCTCT AGAAAGAGAC AAACCAGAGT CCCTGAGTTC TTTAGAAGAA
    GACCAGGAGG CAGTGAGACC TCCAGAAAGA GAAGACCAGG AGCTGCTGAG GTCTCTAGAA
    AAAGAAGACC AGGAGCCACT GAGGTCTCTA GAAGAAGACC AGGAGGCAGT GAGACCTCTA
    GAAGAAGAAA ACCAGGAGCT GCTGAGGTCT CTAGAAAAAG AAAAAGAGGA GTCACTGAGG
    TCTCTAGAAG ACCAGGAGGC AATGAGACCT CTAGAAGAAG AAAACCAGGA GCCGCTGAGG
    TCTCTAGAAA AAGAAAAACA GGAGGCGACG AGACCTCCAG AAGAAGAAAA ACAGGAGCCA
    CTGATGTCTC TAGAAGAAGA CCAGGAGGAA ATGAGAGCTT TAGAAGAAGA CCAGGAGACA
    GTGAGACCTC TAGAAAAAGA AAAACAGGAG TCACTAAGGT CTCTAGAAGA CCACGAGGCA
    ATGAGACCTC TAGAAGAAGA AAACCAGGAG CCGCTGAGGT CTCTAGAAAA AGAAAAACAG
    GAGGTGATGA GACCTCCAGA AGAAGAAAAA CAGGAATTAC TGAGATCTCT AGAAGACCAG
    GAGGCAATGA GATCTCTAGA AGAAGAAGGC CAGATGACAT TGAGCCCTCT AGAAAAAGTG
    AAACCAGAGA CACTAAAGTC TCTTGGAAAA GATCAGGAGA TAGTTAGACC TCTTGAAAAA
    GAGAATCAAG AGTTATTGAG GTCCCTAAAT GAAAAGAGTA CAGAGGCAGT GAGGTCTTTA
    GAAACAGAGA CTCCAGAACC ACTAAAGCCT ACAGAAGAGG AAAACCTGGA AATACTGAAA
    CCTCTAGAAA AGGAAAGTCA AGAGCCACTG GGGTCTGTGG AAGGGAACCA TGAGACACTG
    AGACCCCCAG AGAAGGAGAA TCAAGAGTCA CTGGGCTCTC TGGCAGAGTG GAACGTGGAG
    AATTTGCAGT CTCTAGAGGA GGCCGACAAG GGAAGTCTAA GGCACTTGGA AGAGGAAGAG
    GACGTGGAGC AGGAAGAGAG TCAGGCGTCA CTGAGGCCCC TGGAGGAGCA GGGGCAGGAG
    CTGCCACTCT CTGCACAGCA GCAGAAGTGG GGAGATGCAA TGCAGGGGGA CCAAGAACTG
    GATCAGGAGC GGCCTCCGGG GAGGGCTGGA GTGGACAGTG GGGATGGGGC AGAGCTGGAA
    CTGAAAGAAC TGGATAGCTC CCCTGCGGAG GGGGAGGTGG TGGAGCAGGG TCAGCTGCAG
    CTGACAGCCA CAGGTGAGGC CTGGGGCCCA GGTGAGGGGC AGCCAGGCAG CCCTGAGCCC
    AAAGAGCAGA GGCTCCCAGC TGAGGGTGCC GGAGGGGCAG GAGGCGCTGC GGGCCTCCAG
    GACCCTGAGG AGCAGCCAGA GCAGGTGGGG GCCCTGGGCC TCCCGGCTGC CCAGGGAACA
    TCAGAGGTGA TGGAGCCCGA GTTGGAAGAT GAGGATGTGG CCCCAGGGGG TGGCCGAGCC
    TCCCCAGAGG TCACCTTGGA GTTAGAGAGG GCCATGGGCA GGTCTGAGGG AGTGGAACGG
    GGACCCGAGC AGGAGGCAGT AAAGCTGGAG GACCCAGGTG GCCTGGCCAG AGAGGAGGTG
    ATGGAGCCAC CCCTGGGGGA GGGAGGTATG GCAGCAAAGA AGGTACAGGG CTTGGAAGGG
    CCCAGAAAGG AGCTGGAGGA GGCAGGCACT CTGGAGCCTG AGTCCGGGAA GAGCAGAGAC
    CCGTTGGAGA CTCCTAGGGA CTGGGAGGAG TCAGAATCTG GGGCCCTTGG GAAAACAGAG
    GAGCCAGTCT CAGCTGAGAC CTTGTGGTCT CACGAGGGAA GTGATACCCT CCAGCCCAGG
    CCCCTGGGGT CAGAGGGAGC TGAGGAGGAT GCCAAACCAC TGCTGGGGCC CCCCAGTCTG
    AGGCCCACTG AGTCCTGCTT GCCCAGCCTA ATCCCTGAAG ATGCCCCTGG GCCCCAGCCC
    CTGGCTGAGG GGAACCAAGA AGCCAGCTGG GGGCTGGAGG GCAGGGCTGA GGTCGTAGGA
    AAGGCAGGAG GTGAGCAGGA GGATCTGGGC TCTGGGGGGA TCCCTGAGGG CCTCCAGGAG
    GAAGGGGAGG AGAGCAGAGA AGAGAGCGAG GCGGATGAGC TAGGGGAGAC CCTTCCTGAC
    TCCACTCCCC TAGGCCTCTA CCTCAGGTCC CCCGCTTCCC CCAAGTGGGA CCTGCCTGGA
    GAGCAGAGGC CCTCCCCTCA AGGGGAGCCC AGAAAAGAAG GCTGGGATCC TGTGGTCCCA
    GCCTCCAAGA GCCTTGGGGC CCAGCCCTCA GAGGAGGAAG AAGAGGAGGG AGCTGAGGAG
    GAGGAACGTG GCCATGATTC TGAGCTGTCG GAAGAGTTTG AGGACCTGGG GACTGAGGCT
    TCTCTCCTTC CCGGGGTCCC GGGGGAGGGG GAAGAACCTC TGAGCCAAGT GCCCCAGCTG
    CTCCTGGAGC CTGCAGCCTG GGATCGTGAT GGTGAATCTG ACGGATTTGC AGATGAGGAA
    GAGAGCGGGG AGGAGGGCGA GGAAGAAGAT GAAGAAGAGG GGAGGGAGCC AGGGCCAGGG
    CATTGGGGGC CAGCGCCCTC TGTGGGCAGC CTCCCCGCCC TGAGCGGCCC TCAGGGAGGG
    CACCTCCTGG AGTCTGAGAC CGTGGGTGTC AGTGTCCCCT GGGACGATGG CCCAAGGGGC
    GCGGCAGCCG ACGCCCCCGT GACTGCCCTG GAGACCGAGT CCCAGAACAG CACTGAGGCC
    TCAGGCTCAG AGGAAGAGTC TGATGCTGCT CCCCTGGAGA CGGAGGACCA GGTCCCTGGC
    CCTCTGGGGA CCCTCAGCGG GGTGGAGGAC ACCCTTGATG GTGGCCCAGG CCCCAGCCTG
    AAGGAAGAGC TGGAGCGCAT GAATGGGGGC GTGGTGAACG GGCTGGAGCA GTCCGAGGGG
    GGAGGCCAGG GGAAACCGGG GGCTGCTGGG GGGGACCGAG GGAGCCCCTC AGAGGAGGAG
    GGGGCTTCCC TGAAGGCCCC ATGGGCAGGG GCTCCTCTTC ACCTGGGCCA AGGCCAGTTC
    CTGAAGTTCA GTCAGAGAGA AGGTGATGGA GACTCCTGGT CCTCAGGGGA GGACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa232778
    Clone ID Related Accession (Same CDS sequence) XM_024989303.1
    Accession Version XM_024989303.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5178bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product nestin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGGAGGGCT GCCTGGGGGA GGAATCTTTT CAGATGTGGG AGCTCAACCG GCGCCTGGAG 
    GCCTACCTGG CCCGGGTCAA GGCGCTAGAA GAGCAGAATG AGCTGCTCAG CGCGGAGCTC
    GGGGGTCTCC GGGCACAGGC CGGGGACACC TCCTGGAGGG CTCGTGCCGA CGACGAGCTG
    GCGGCCCTAC GGGCCCTCGT CGACCAGCGC TGGCGGGAGA AGCACGCGGC CGAGGTGGCG
    CGCGACAACC TGGCAGAAGA GGTGGAGGGC GTGGCGAGTC GGTGCCAGCA GCTGCGGCTG
    GCCCGGGAGC GGACCGCGGA GGAGGTGGCC CGCAGCCGGC GTGCGGTCGA GGCCGAGAAA
    TGCGCCCAGG CCTGGCTGAG CACCCAGGCT GCGGAGCTGG AGCGCGAGCT GGAGGCTCTG
    CGCGTGGCGC AAGAGGAGGA GCGCGCCGGC CTGAACGCGC AGGCTGCCTG CGCCCCCCGC
    GGCTCCGCTC CGCCCCGGGG TCCCCCCGCG CCAGCCCCGG AGGTGGAGGA GCTGGCTCAG
    CGGCTGGGCG AGGCGTGGCG TGGGGCAGTG CGCGGCTACC AGGAACGCGT GGCGCACATG
    GAGACGTCGC TGGGCCAGGC CCGCGAGCGG CTAGGCCACG CGATGCAGGG CGCCCGCGAG
    GGTCGCCTGG AGCTGCAGCA ACTCCAGGCA GAGCGCAGCG GCCTTCAGGA GCGCAGGGCC
    GCGCTGGAGC AGAGGCTGGA GGGCCGCTGG CAGGAGCGGC TGCGGACCAC TGAGCAGTTC
    CAGCTGGCCG TGGAGGCCCT GGAGCAGGAG AAACAAGGCC TCCAGAGCCA AATCGCCCAG
    GTCCTGGAAG GTCGGCAGCA GCTGGCACAC CTCAAGATGT CCCTCAGCCT GGAGGTGGCC
    ACATACAGGA CCCTCCTAGA GGCTGAGAAC TCACGGCTGC AGACACCTGC AAGCGGTTCC
    AAGGCTTCCC TCAGCTTTCT GGACCCTAAG CTGGAGCTAC ATTTCCCTGG GACCCCAGAG
    GGCCGGCGTC CGGGACCTCT GCTTTCTGTC CTGAGCCCTA CTCCCCTCTC CTCACCTTTG
    CCTGGTACCC TTGAAACACC TGTGCCAACC TTTCTGAAGA GCCAGGAATT CCTTCAGGCT
    CGCACCCCGA CCTCAGCCAG CACCCCCATC CCACCCACCC CTCAGGCTCC CTGCCCTGCT
    GTAGATGCCG AGATCAGAGC CCAGGATGCC CCTGTGTCTC TGCCCCAAGC ACGAGTTGGG
    AGGCAACAGG TGCCAGAAGT CATGTGGGCT GAAGCCAAGG TGGCCATCCC CGCCAGCGTC
    CTGCCAGGAC CAGAGGAGCC TGGGGGCCAG CAACAAGAGC CCAGTCCAAG CCAGTCCCCT
    GAAGATCATG CCTCCCTGGC TCCAGCCCTC AGCCCTGACC ACTCCAGTCT AGAGACCAAA
    GATGGAGAAC CCAGTGCATC TAGAGAGTCC AGCAGATCCC AGGAGGAAGA TGAAGGCCAA
    CTGTGGGGGC TGACAGAGAA AGAAACAGTG GTAGAGGCCA AAGCAGTGAG CAGCTTGCAG
    CAGGAAACGT GTCAAGAAGA GGCGGGTCTG GACGTGAAAG AAATCCAAGA TGCCCAGGGC
    CCTTTGGAAA AAGAAGCTCT GAACTCTCTG GAAGAAGAGA TTCAAAAGCC ATCGATTCCA
    CTGGAAAAAC AGAGCCATGA GACTATAAGA TCTCTAGAGA AGGAGAATCT GGAATCTCTG
    AGGTCTTTGG AAGAAGAGAA CTTAGAAACA CTAAAAATAC TAGAAAAGGA GAATCAAGAA
    TTATTGAAGT CTTTAGAAGG AAAGGAAACG GAGGTAATGA GATCTCTAGA AAAAGAAACT
    CTAGAACTAC TTAAGCCTAT AGGAAAAGAG GATCCACAGA CATTGCCATC TCTAGGAAAG
    GAGAATCAAG AAATAATGAG GTCTCTTGAA GTTAATGTAG AGACATTCTT ATATCCAGGA
    AAGGAAAATC AAGAATTAGT GAGGTCTTTA GAAGAGGAGA ACGCTGAGTC ATTGAGAACT
    CTAGAAAAGG AGAGTCAAGA GCCACTGAGA TGTCAAGAAG TAGAGAACCA GGGAACACTG
    AGACTCCTAG CCAAAGACAA TCAGGAGCCA CTGAGGTCTC TAGAAGAAGA AGACCAGGAG
    ACGTCAAGAC CGCTAGGGAA AGAGAATCAT GAATCCCTGC GGGCTCCAGA AGATGAGAAT
    CATGAGGCTT TGAGACCTCT AGAAAGAGAC AAACCAGAGT CCCTGAGTTC TTTAGAAGAA
    GACCAGGAGG CAGTGAGACC TCCAGAAAGA GAAGACCAGG AGCTGCTGAG GTCTCTAGAA
    AAAGAAGACC AGGAGCCACT GAGGTCTCTA GAAGAAGACC AGGAGGCAGT GAGACCTCTA
    GAAGAAGAAA ACCAGGAGCT GCTGAGGTCT CTAGAAAAAG AAAAAGAGGA GTCACTGAGG
    TCTCTAGAAG ACCAGGAGGC AATGAGACCT CTAGAAGAAG AAAACCAGGA GCCGCTGAGG
    TCTCTAGAAG ACCAGGAGGC AATGAGACCT CTAGAAGAAG AAAACCAGGA GCCGCTGAGG
    TCTCTAGAAA AAGAAAAACA GGAGGTGATG AGACCTCCAG AAGAAGAAAA ACAGGAATTA
    CTGAGATCTC TAGAAGACCA GGAGGCAATG AGATCTCTAG AAGAAGAAGG CCAGATGACA
    TTGAGCCCTC TAGAAAAAGT GAAACCAGAG ACACTAAAGT CTCTTGGAAA AGATCAGGAG
    ATAGTTAGAC CTCTTGAAAA AGAGAATCAA GAGTTATTGA GGTCCCTAAA TGAAAAGAGT
    ACAGAGGCAG TGAGGTCTTT AGAAACAGAG ACTCCAGAAC CACTAAAGCC TACAGAAGAG
    GAAAACCTGG AAATACTGAA ACCTCTAGAA AAGGAAAGTC AAGAGCCACT GGGGTCTGTG
    GAAGGGAACC ATGAGACACT GAGACCCCCA GAGAAGGAGA ATCAAGAGTC ACTGGGCTCT
    CTGGCAGAGT GGAACGTGGA GAATTTGCAG TCTCTAGAGG AGGCCGACAA GGGAAGTCTA
    AGGCACTTGG AAGAGGAAGA GGACGTGGAG CAGGAAGAGA GTCAGGCGTC ACTGAGGCCC
    CTGGAGGAGC AGGGGCAGGA GCTGCCACTC TCTGCACAGC AGCAGAAGTG GGGAGATGCA
    ATGCAGGGGG ACCAAGAACT GGATCAGGAG CGGCCTCCGG GGAGGGCTGG AGTGGACAGT
    GGGGATGGGG CAGAGCTGGA ACTGAAAGAA CTGGATAGCT CCCCTGCGGA GGGGGAGGTG
    GTGGAGCAGG GTCAGCTGCA GCTGACAGCC ACAGGTGAGG CCTGGGGCCC AGGTGAGGGG
    CAGCCAGGCA GCCCTGAGCC CAAAGAGCAG AGGCTCCCAG CTGAGGGTGC CGGAGGGGCA
    GGAGGCGCTG CGGGCCTCCA GGACCCTGAG GAGCAGCCAG AGCAGGTGGG GGCCCTGGGC
    CTCCCGGCTG CCCAGGGAAC ATCAGAGGTG ATGGAGCCCG AGTTGGAAGA TGAGGATGTG
    GCCCCAGGGG GTGGCCGAGC CTCCCCAGAG GTCACCTTGG AGTTAGAGAG GGCCATGGGC
    AGGTCTGAGG GAGTGGAACG GGGACCCGAG CAGGAGGCAG TAAAGCTGGA GGACCCAGGT
    GGCCTGGCCA GAGAGGAGGT GATGGAGCCA CCCCTGGGGG AGGGAGGTAT GGCAGCAAAG
    AAGGTACAGG GCTTGGAAGG GCCCAGAAAG GAGCTGGAGG AGGCAGGCAC TCTGGAGCCT
    GAGTCCGGGA AGAGCAGAGA CCCGTTGGAG ACTCCTAGGG ACTGGGAGGA GTCAGAATCT
    GGGGCCCTTG GGAAAACAGA GGAGCCAGTC TCAGCTGAGA CCTTGTGGTC TCACGAGGGA
    AGTGATACCC TCCAGCCCAG GCCCCTGGGG TCAGAGGGAG CTGAGGAGGA TGCCAAACCA
    CTGCTGGGGC CCCCCAGTCT GAGGCCCACT GAGTCCTGCT TGCCCAGCCT AATCCCTGAA
    GATGCCCCTG GGCCCCAGCC CCTGGCTGAG GGGAACCAAG AAGCCAGCTG GGGGCTGGAG
    GGCAGGGCTG AGGTCGTAGG AAAGGCAGGA GGTGAGCAGG AGGATCTGGG CTCTGGGGGG
    ATCCCTGAGG GCCTCCAGGA GGAAGGGGAG GAGAGCAGAG AAGAGAGCGA GGCGGATGAG
    CTAGGGGAGA CCCTTCCTGA CTCCACTCCC CTAGGCCTCT ACCTCAGGTC CCCCGCTTCC
    CCCAAGTGGG ACCTGCCTGG AGAGCAGAGG CCCTCCCCTC AAGGGGAGCC CAGAAAAGAA
    GGCTGGGATC CTGTGGTCCC AGCCTCCAAG AGCCTTGGGG CCCAGCCCTC AGAGGAGGAA
    GAAGAGGAGG GAGCTGAGGA GGAGGAACGT GGCCATGATT CTGAGCTGTC GGAAGAGTTT
    GAGGACCTGG GGACTGAGGC TTCTCTCCTT CCCGGGGTCC CGGGGGAGGG GGAAGAACCT
    CTGAGCCAAG TGCCCCAGCT GCTCCTGGAG CCTGCAGCCT GGGATCGTGA TGGTGAATCT
    GACGGATTTG CAGATGAGGA AGAGAGCGGG GAGGAGGGCG AGGAAGAAGA TGAAGAAGAG
    GGGAGGGAGC CAGGGCCAGG GCATTGGGGG CCAGCGCCCT CTGTGGGCAG CCTCCCCGCC
    CTGAGCGGCC CTCAGGGAGG GCACCTCCTG GAGTCTGAGA CCGTGGGTGT CAGTGTCCCC
    TGGGACGATG GCCCAAGGGG CGCGGCAGCC GACGCCCCCG TGACTGCCCT GGAGACCGAG
    TCCCAGAACA GCACTGAGGC CTCAGGCTCA GAGGAAGAGT CTGATGCTGC TCCCCTGGAG
    ACGGAGGACC AGGTCCCTGG CCCTCTGGGG ACCCTCAGCG GGGTGGAGGA CACCCTTGAT
    GGTGGCCCAG GCCCCAGCCT GAAGGAAGAG CTGGAGCGCA TGAATGGGGG CGTGGTGAAC
    GGGCTGGAGC AGTCCGAGGG GGGAGGCCAG GGGAAACCGG GGGCTGCTGG GGGGGACCGA
    GGGAGCCCCT CAGAGGAGGA GGGGGCTTCC CTGAAGGCCC CATGGGCAGG GGCTCCTCTT
    CACCTGGGCC AAGGCCAGTT CCTGAAGTTC AGTCAGAGAG AAGGTGATGG AGACTCCTGG
    TCCTCAGGGG AGGACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024845071.1
    CDS162..5339
    Translation

    Target ORF information:

    RefSeq Version XM_024989303.1
    Organism Bos taurus(cattle)
    Definition Bos taurus nestin (NES), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024989303.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGGAGGGCT GCCTGGGGGA GGAATCTTTT CAGATGTGGG AGCTCAACCG GCGCCTGGAG 
    GCCTACCTGG CCCGGGTCAA GGCGCTAGAA GAGCAGAATG AGCTGCTCAG CGCGGAGCTC
    GGGGGTCTCC GGGCACAGGC CGGGGACACC TCCTGGAGGG CTCGTGCCGA CGACGAGCTG
    GCGGCCCTAC GGGCCCTCGT CGACCAGCGC TGGCGGGAGA AGCACGCGGC CGAGGTGGCG
    CGCGACAACC TGGCAGAAGA GGTGGAGGGC GTGGCGAGTC GGTGCCAGCA GCTGCGGCTG
    GCCCGGGAGC GGACCGCGGA GGAGGTGGCC CGCAGCCGGC GTGCGGTCGA GGCCGAGAAA
    TGCGCCCAGG CCTGGCTGAG CACCCAGGCT GCGGAGCTGG AGCGCGAGCT GGAGGCTCTG
    CGCGTGGCGC AAGAGGAGGA GCGCGCCGGC CTGAACGCGC AGGCTGCCTG CGCCCCCCGC
    GGCTCCGCTC CGCCCCGGGG TCCCCCCGCG CCAGCCCCGG AGGTGGAGGA GCTGGCTCAG
    CGGCTGGGCG AGGCGTGGCG TGGGGCAGTG CGCGGCTACC AGGAACGCGT GGCGCACATG
    GAGACGTCGC TGGGCCAGGC CCGCGAGCGG CTAGGCCACG CGATGCAGGG CGCCCGCGAG
    GGTCGCCTGG AGCTGCAGCA ACTCCAGGCA GAGCGCAGCG GCCTTCAGGA GCGCAGGGCC
    GCGCTGGAGC AGAGGCTGGA GGGCCGCTGG CAGGAGCGGC TGCGGACCAC TGAGCAGTTC
    CAGCTGGCCG TGGAGGCCCT GGAGCAGGAG AAACAAGGCC TCCAGAGCCA AATCGCCCAG
    GTCCTGGAAG GTCGGCAGCA GCTGGCACAC CTCAAGATGT CCCTCAGCCT GGAGGTGGCC
    ACATACAGGA CCCTCCTAGA GGCTGAGAAC TCACGGCTGC AGACACCTGC AAGCGGTTCC
    AAGGCTTCCC TCAGCTTTCT GGACCCTAAG CTGGAGCTAC ATTTCCCTGG GACCCCAGAG
    GGCCGGCGTC CGGGACCTCT GCTTTCTGTC CTGAGCCCTA CTCCCCTCTC CTCACCTTTG
    CCTGGTACCC TTGAAACACC TGTGCCAACC TTTCTGAAGA GCCAGGAATT CCTTCAGGCT
    CGCACCCCGA CCTCAGCCAG CACCCCCATC CCACCCACCC CTCAGGCTCC CTGCCCTGCT
    GTAGATGCCG AGATCAGAGC CCAGGATGCC CCTGTGTCTC TGCCCCAAGC ACGAGTTGGG
    AGGCAACAGG TGCCAGAAGT CATGTGGGCT GAAGCCAAGG TGGCCATCCC CGCCAGCGTC
    CTGCCAGGAC CAGAGGAGCC TGGGGGCCAG CAACAAGAGC CCAGTCCAAG CCAGTCCCCT
    GAAGATCATG CCTCCCTGGC TCCAGCCCTC AGCCCTGACC ACTCCAGTCT AGAGACCAAA
    GATGGAGAAC CCAGTGCATC TAGAGAGTCC AGCAGATCCC AGGAGGAAGA TGAAGGCCAA
    CTGTGGGGGC TGACAGAGAA AGAAACAGTG GTAGAGGCCA AAGCAGTGAG CAGCTTGCAG
    CAGGAAACGT GTCAAGAAGA GGCGGGTCTG GACGTGAAAG AAATCCAAGA TGCCCAGGGC
    CCTTTGGAAA AAGAAGCTCT GAACTCTCTG GAAGAAGAGA TTCAAAAGCC ATCGATTCCA
    CTGGAAAAAC AGAGCCATGA GACTATAAGA TCTCTAGAGA AGGAGAATCT GGAATCTCTG
    AGGTCTTTGG AAGAAGAGAA CTTAGAAACA CTAAAAATAC TAGAAAAGGA GAATCAAGAA
    TTATTGAAGT CTTTAGAAGG AAAGGAAACG GAGGTAATGA GATCTCTAGA AAAAGAAACT
    CTAGAACTAC TTAAGCCTAT AGGAAAAGAG GATCCACAGA CATTGCCATC TCTAGGAAAG
    GAGAATCAAG AAATAATGAG GTCTCTTGAA GTTAATGTAG AGACATTCTT ATATCCAGGA
    AAGGAAAATC AAGAATTAGT GAGGTCTTTA GAAGAGGAGA ACGCTGAGTC ATTGAGAACT
    CTAGAAAAGG AGAGTCAAGA GCCACTGAGA TGTCAAGAAG TAGAGAACCA GGGAACACTG
    AGACTCCTAG CCAAAGACAA TCAGGAGCCA CTGAGGTCTC TAGAAGAAGA AGACCAGGAG
    ACGTCAAGAC CGCTAGGGAA AGAGAATCAT GAATCCCTGC GGGCTCCAGA AGATGAGAAT
    CATGAGGCTT TGAGACCTCT AGAAAGAGAC AAACCAGAGT CCCTGAGTTC TTTAGAAGAA
    GACCAGGAGG CAGTGAGACC TCCAGAAAGA GAAGACCAGG AGCTGCTGAG GTCTCTAGAA
    AAAGAAGACC AGGAGCCACT GAGGTCTCTA GAAGAAGACC AGGAGGCAGT GAGACCTCTA
    GAAGAAGAAA ACCAGGAGCT GCTGAGGTCT CTAGAAAAAG AAAAAGAGGA GTCACTGAGG
    TCTCTAGAAG ACCAGGAGGC AATGAGACCT CTAGAAGAAG AAAACCAGGA GCCGCTGAGG
    TCTCTAGAAG ACCAGGAGGC AATGAGACCT CTAGAAGAAG AAAACCAGGA GCCGCTGAGG
    TCTCTAGAAA AAGAAAAACA GGAGGTGATG AGACCTCCAG AAGAAGAAAA ACAGGAATTA
    CTGAGATCTC TAGAAGACCA GGAGGCAATG AGATCTCTAG AAGAAGAAGG CCAGATGACA
    TTGAGCCCTC TAGAAAAAGT GAAACCAGAG ACACTAAAGT CTCTTGGAAA AGATCAGGAG
    ATAGTTAGAC CTCTTGAAAA AGAGAATCAA GAGTTATTGA GGTCCCTAAA TGAAAAGAGT
    ACAGAGGCAG TGAGGTCTTT AGAAACAGAG ACTCCAGAAC CACTAAAGCC TACAGAAGAG
    GAAAACCTGG AAATACTGAA ACCTCTAGAA AAGGAAAGTC AAGAGCCACT GGGGTCTGTG
    GAAGGGAACC ATGAGACACT GAGACCCCCA GAGAAGGAGA ATCAAGAGTC ACTGGGCTCT
    CTGGCAGAGT GGAACGTGGA GAATTTGCAG TCTCTAGAGG AGGCCGACAA GGGAAGTCTA
    AGGCACTTGG AAGAGGAAGA GGACGTGGAG CAGGAAGAGA GTCAGGCGTC ACTGAGGCCC
    CTGGAGGAGC AGGGGCAGGA GCTGCCACTC TCTGCACAGC AGCAGAAGTG GGGAGATGCA
    ATGCAGGGGG ACCAAGAACT GGATCAGGAG CGGCCTCCGG GGAGGGCTGG AGTGGACAGT
    GGGGATGGGG CAGAGCTGGA ACTGAAAGAA CTGGATAGCT CCCCTGCGGA GGGGGAGGTG
    GTGGAGCAGG GTCAGCTGCA GCTGACAGCC ACAGGTGAGG CCTGGGGCCC AGGTGAGGGG
    CAGCCAGGCA GCCCTGAGCC CAAAGAGCAG AGGCTCCCAG CTGAGGGTGC CGGAGGGGCA
    GGAGGCGCTG CGGGCCTCCA GGACCCTGAG GAGCAGCCAG AGCAGGTGGG GGCCCTGGGC
    CTCCCGGCTG CCCAGGGAAC ATCAGAGGTG ATGGAGCCCG AGTTGGAAGA TGAGGATGTG
    GCCCCAGGGG GTGGCCGAGC CTCCCCAGAG GTCACCTTGG AGTTAGAGAG GGCCATGGGC
    AGGTCTGAGG GAGTGGAACG GGGACCCGAG CAGGAGGCAG TAAAGCTGGA GGACCCAGGT
    GGCCTGGCCA GAGAGGAGGT GATGGAGCCA CCCCTGGGGG AGGGAGGTAT GGCAGCAAAG
    AAGGTACAGG GCTTGGAAGG GCCCAGAAAG GAGCTGGAGG AGGCAGGCAC TCTGGAGCCT
    GAGTCCGGGA AGAGCAGAGA CCCGTTGGAG ACTCCTAGGG ACTGGGAGGA GTCAGAATCT
    GGGGCCCTTG GGAAAACAGA GGAGCCAGTC TCAGCTGAGA CCTTGTGGTC TCACGAGGGA
    AGTGATACCC TCCAGCCCAG GCCCCTGGGG TCAGAGGGAG CTGAGGAGGA TGCCAAACCA
    CTGCTGGGGC CCCCCAGTCT GAGGCCCACT GAGTCCTGCT TGCCCAGCCT AATCCCTGAA
    GATGCCCCTG GGCCCCAGCC CCTGGCTGAG GGGAACCAAG AAGCCAGCTG GGGGCTGGAG
    GGCAGGGCTG AGGTCGTAGG AAAGGCAGGA GGTGAGCAGG AGGATCTGGG CTCTGGGGGG
    ATCCCTGAGG GCCTCCAGGA GGAAGGGGAG GAGAGCAGAG AAGAGAGCGA GGCGGATGAG
    CTAGGGGAGA CCCTTCCTGA CTCCACTCCC CTAGGCCTCT ACCTCAGGTC CCCCGCTTCC
    CCCAAGTGGG ACCTGCCTGG AGAGCAGAGG CCCTCCCCTC AAGGGGAGCC CAGAAAAGAA
    GGCTGGGATC CTGTGGTCCC AGCCTCCAAG AGCCTTGGGG CCCAGCCCTC AGAGGAGGAA
    GAAGAGGAGG GAGCTGAGGA GGAGGAACGT GGCCATGATT CTGAGCTGTC GGAAGAGTTT
    GAGGACCTGG GGACTGAGGC TTCTCTCCTT CCCGGGGTCC CGGGGGAGGG GGAAGAACCT
    CTGAGCCAAG TGCCCCAGCT GCTCCTGGAG CCTGCAGCCT GGGATCGTGA TGGTGAATCT
    GACGGATTTG CAGATGAGGA AGAGAGCGGG GAGGAGGGCG AGGAAGAAGA TGAAGAAGAG
    GGGAGGGAGC CAGGGCCAGG GCATTGGGGG CCAGCGCCCT CTGTGGGCAG CCTCCCCGCC
    CTGAGCGGCC CTCAGGGAGG GCACCTCCTG GAGTCTGAGA CCGTGGGTGT CAGTGTCCCC
    TGGGACGATG GCCCAAGGGG CGCGGCAGCC GACGCCCCCG TGACTGCCCT GGAGACCGAG
    TCCCAGAACA GCACTGAGGC CTCAGGCTCA GAGGAAGAGT CTGATGCTGC TCCCCTGGAG
    ACGGAGGACC AGGTCCCTGG CCCTCTGGGG ACCCTCAGCG GGGTGGAGGA CACCCTTGAT
    GGTGGCCCAG GCCCCAGCCT GAAGGAAGAG CTGGAGCGCA TGAATGGGGG CGTGGTGAAC
    GGGCTGGAGC AGTCCGAGGG GGGAGGCCAG GGGAAACCGG GGGCTGCTGG GGGGGACCGA
    GGGAGCCCCT CAGAGGAGGA GGGGGCTTCC CTGAAGGCCC CATGGGCAGG GGCTCCTCTT
    CACCTGGGCC AAGGCCAGTT CCTGAAGTTC AGTCAGAGAG AAGGTGATGG AGACTCCTGG
    TCCTCAGGGG AGGACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa232779
    Clone ID Related Accession (Same CDS sequence) XM_024989307.1
    Accession Version XM_024989307.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5118bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product nestin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGAGGGCT GCCTGGGGGA GGAATCTTTT CAGATGTGGG AGCTCAACCG GCGCCTGGAG 
    GCCTACCTGG CCCGGGTCAA GGCGCTAGAA GAGCAGAATG AGCTGCTCAG CGCGGAGCTC
    GGGGGTCTCC GGGCACAGGC CGGGGACACC TCCTGGAGGG CTCGTGCCGA CGACGAGCTG
    GCGGCCCTAC GGGCCCTCGT CGACCAGCGC TGGCGGGAGA AGCACGCGGC CGAGGTGGCG
    CGCGACAACC TGGCAGAAGA GGTGGAGGGC GTGGCGAGTC GGTGCCAGCA GCTGCGGCTG
    GCCCGGGAGC GGACCGCGGA GGAGGTGGCC CGCAGCCGGC GTGCGGTCGA GGCCGAGAAA
    TGCGCCCAGG CCTGGCTGAG CACCCAGGCT GCGGAGCTGG AGCGCGAGCT GGAGGCTCTG
    CGCGTGGCGC AAGAGGAGGA GCGCGCCGGC CTGAACGCGC AGGCTGCCTG CGCCCCCCGC
    GGCTCCGCTC CGCCCCGGGG TCCCCCCGCG CCAGCCCCGG AGGTGGAGGA GCTGGCTCAG
    CGGCTGGGCG AGGCGTGGCG TGGGGCAGTG CGCGGCTACC AGGAACGCGT GGCGCACATG
    GAGACGTCGC TGGGCCAGGC CCGCGAGCGG CTAGGCCACG CGATGCAGGG CGCCCGCGAG
    GGTCGCCTGG AGCTGCAGCA ACTCCAGGCA GAGCGCAGCG GCCTTCAGGA GCGCAGGGCC
    GCGCTGGAGC AGAGGCTGGA GGGCCGCTGG CAGGAGCGGC TGCGGACCAC TGAGCAGTTC
    CAGCTGGCCG TGGAGGCCCT GGAGCAGGAG AAACAAGGCC TCCAGAGCCA AATCGCCCAG
    GTCCTGGAAG GTCGGCAGCA GCTGGCACAC CTCAAGATGT CCCTCAGCCT GGAGGTGGCC
    ACATACAGGA CCCTCCTAGA GGCTGAGAAC TCACGGCTGC AGACACCTGC AAGCGGTTCC
    AAGGCTTCCC TCAGCTTTCT GGACCCTAAG CTGGAGCTAC ATTTCCCTGG GACCCCAGAG
    GGCCGGCGTC CGGGACCTCT GCTTTCTGTC CTGAGCCCTA CTCCCCTCTC CTCACCTTTG
    CCTGGTACCC TTGAAACACC TGTGCCAACC TTTCTGAAGA GCCAGGAATT CCTTCAGGCT
    CGCACCCCGA CCTCAGCCAG CACCCCCATC CCACCCACCC CTCAGGCTCC CTGCCCTGCT
    GTAGATGCCG AGATCAGAGC CCAGGATGCC CCTGTGTCTC TGCCCCAAGC ACGAGTTGGG
    AGGCAACAGG TGCCAGAAGT CATGTGGGCT GAAGCCAAGG TGGCCATCCC CGCCAGCGTC
    CTGCCAGGAC CAGAGGAGCC TGGGGGCCAG CAACAAGAGC CCAGTCCAAG CCAGTCCCCT
    GAAGATCATG CCTCCCTGGC TCCAGCCCTC AGCCCTGACC ACTCCAGTCT AGAGACCAAA
    GATGGAGAAC CCAGTGCATC TAGAGAGTCC AGCAGATCCC AGGAGGAAGA TGAAGGCCAA
    CTGTGGGGGC TGACAGAGAA AGAAACAGTG GTAGAGGCCA AAGCAGTGAG CAGCTTGCAG
    CAGGAAACGT GTCAAGAAGA GGCGGGTCTG GACGTGAAAG AAATCCAAGA TGCCCAGGGC
    CCTTTGGAAA AAGAAGCTCT GAACTCTCTG GAAGAAGAGA TTCAAAAGCC ATCGATTCCA
    CTGGAAAAAC AGAGCCATGA GACTATAAGA TCTCTAGAGA AGGAGAATCT GGAATCTCTG
    AGGTCTTTGG AAGAAGAGAA CTTAGAAACA CTAAAAATAC TAGAAAAGGA GAATCAAGAA
    TTATTGAAGT CTTTAGAAGG AAAGGAAACG GAGGTAATGA GATCTCTAGA AAAAGAAACT
    CTAGAACTAC TTAAGCCTAT AGGAAAAGAG GATCCACAGA CATTGCCATC TCTAGGAAAG
    GAGAATCAAG AAATAATGAG GTCTCTTGAA GTTAATGTAG AGACATTCTT ATATCCAGGA
    AAGGAAAATC AAGAATTAGT GAGGTCTTTA GAAGAGGAGA ACGCTGAGTC ATTGAGAACT
    CTAGAAAAGG AGAGTCAAGA GCCACTGAGA TGTCAAGAAG TAGAGAACCA GGGAACACTG
    AGACTCCTAG CCAAAGACAA TCAGGAGCCA CTGAGGTCTC TAGAAGAAGA AGACCAGGAG
    ACGTCAAGAC CGCTAGGGAA AGAGAATCAT GAATCCCTGC GGGCTCCAGA AGATGAGAAT
    CATGAGGCTT TGAGACCTCT AGAAAGAGAC AAACCAGAGT CCCTGAGTTC TTTAGAAGAA
    GACCAGGAGG CAGTGAGACC TCCAGAAAGA GAAGACCAGG AGCTGCTGAG GTCTCTAGAA
    AAAGAAGACC AGGAGCCACT GAGGTCTCTA GAAGAAGACC AGGAGGCAGT GAGACCTCTA
    GAAGAAGAAA ACCAGGAGCT GCTGAGGTCT CTAGAAAAAG AAAAAGAGGA GTCACTGAGG
    TCTCTAGAAG ACCAGGAGGC AATGAGACCT CTAGAAGAAG AAAACCAGGA GCCGCTGAGG
    TCTCTAGAAA AAGAAAAACA GGAGGTGATG AGACCTCCAG AAGAAGAAAA ACAGGAATTA
    CTGAGATCTC TAGAAGACCA GGAGGCAATG AGATCTCTAG AAGAAGAAGG CCAGATGACA
    TTGAGCCCTC TAGAAAAAGT GAAACCAGAG ACACTAAAGT CTCTTGGAAA AGATCAGGAG
    ATAGTTAGAC CTCTTGAAAA AGAGAATCAA GAGTTATTGA GGTCCCTAAA TGAAAAGAGT
    ACAGAGGCAG TGAGGTCTTT AGAAACAGAG ACTCCAGAAC CACTAAAGCC TACAGAAGAG
    GAAAACCTGG AAATACTGAA ACCTCTAGAA AAGGAAAGTC AAGAGCCACT GGGGTCTGTG
    GAAGGGAACC ATGAGACACT GAGACCCCCA GAGAAGGAGA ATCAAGAGTC ACTGGGCTCT
    CTGGCAGAGT GGAACGTGGA GAATTTGCAG TCTCTAGAGG AGGCCGACAA GGGAAGTCTA
    AGGCACTTGG AAGAGGAAGA GGACGTGGAG CAGGAAGAGA GTCAGGCGTC ACTGAGGCCC
    CTGGAGGAGC AGGGGCAGGA GCTGCCACTC TCTGCACAGC AGCAGAAGTG GGGAGATGCA
    ATGCAGGGGG ACCAAGAACT GGATCAGGAG CGGCCTCCGG GGAGGGCTGG AGTGGACAGT
    GGGGATGGGG CAGAGCTGGA ACTGAAAGAA CTGGATAGCT CCCCTGCGGA GGGGGAGGTG
    GTGGAGCAGG GTCAGCTGCA GCTGACAGCC ACAGGTGAGG CCTGGGGCCC AGGTGAGGGG
    CAGCCAGGCA GCCCTGAGCC CAAAGAGCAG AGGCTCCCAG CTGAGGGTGC CGGAGGGGCA
    GGAGGCGCTG CGGGCCTCCA GGACCCTGAG GAGCAGCCAG AGCAGGTGGG GGCCCTGGGC
    CTCCCGGCTG CCCAGGGAAC ATCAGAGGTG ATGGAGCCCG AGTTGGAAGA TGAGGATGTG
    GCCCCAGGGG GTGGCCGAGC CTCCCCAGAG GTCACCTTGG AGTTAGAGAG GGCCATGGGC
    AGGTCTGAGG GAGTGGAACG GGGACCCGAG CAGGAGGCAG TAAAGCTGGA GGACCCAGGT
    GGCCTGGCCA GAGAGGAGGT GATGGAGCCA CCCCTGGGGG AGGGAGGTAT GGCAGCAAAG
    AAGGTACAGG GCTTGGAAGG GCCCAGAAAG GAGCTGGAGG AGGCAGGCAC TCTGGAGCCT
    GAGTCCGGGA AGAGCAGAGA CCCGTTGGAG ACTCCTAGGG ACTGGGAGGA GTCAGAATCT
    GGGGCCCTTG GGAAAACAGA GGAGCCAGTC TCAGCTGAGA CCTTGTGGTC TCACGAGGGA
    AGTGATACCC TCCAGCCCAG GCCCCTGGGG TCAGAGGGAG CTGAGGAGGA TGCCAAACCA
    CTGCTGGGGC CCCCCAGTCT GAGGCCCACT GAGTCCTGCT TGCCCAGCCT AATCCCTGAA
    GATGCCCCTG GGCCCCAGCC CCTGGCTGAG GGGAACCAAG AAGCCAGCTG GGGGCTGGAG
    GGCAGGGCTG AGGTCGTAGG AAAGGCAGGA GGTGAGCAGG AGGATCTGGG CTCTGGGGGG
    ATCCCTGAGG GCCTCCAGGA GGAAGGGGAG GAGAGCAGAG AAGAGAGCGA GGCGGATGAG
    CTAGGGGAGA CCCTTCCTGA CTCCACTCCC CTAGGCCTCT ACCTCAGGTC CCCCGCTTCC
    CCCAAGTGGG ACCTGCCTGG AGAGCAGAGG CCCTCCCCTC AAGGGGAGCC CAGAAAAGAA
    GGCTGGGATC CTGTGGTCCC AGCCTCCAAG AGCCTTGGGG CCCAGCCCTC AGAGGAGGAA
    GAAGAGGAGG GAGCTGAGGA GGAGGAACGT GGCCATGATT CTGAGCTGTC GGAAGAGTTT
    GAGGACCTGG GGACTGAGGC TTCTCTCCTT CCCGGGGTCC CGGGGGAGGG GGAAGAACCT
    CTGAGCCAAG TGCCCCAGCT GCTCCTGGAG CCTGCAGCCT GGGATCGTGA TGGTGAATCT
    GACGGATTTG CAGATGAGGA AGAGAGCGGG GAGGAGGGCG AGGAAGAAGA TGAAGAAGAG
    GGGAGGGAGC CAGGGCCAGG GCATTGGGGG CCAGCGCCCT CTGTGGGCAG CCTCCCCGCC
    CTGAGCGGCC CTCAGGGAGG GCACCTCCTG GAGTCTGAGA CCGTGGGTGT CAGTGTCCCC
    TGGGACGATG GCCCAAGGGG CGCGGCAGCC GACGCCCCCG TGACTGCCCT GGAGACCGAG
    TCCCAGAACA GCACTGAGGC CTCAGGCTCA GAGGAAGAGT CTGATGCTGC TCCCCTGGAG
    ACGGAGGACC AGGTCCCTGG CCCTCTGGGG ACCCTCAGCG GGGTGGAGGA CACCCTTGAT
    GGTGGCCCAG GCCCCAGCCT GAAGGAAGAG CTGGAGCGCA TGAATGGGGG CGTGGTGAAC
    GGGCTGGAGC AGTCCGAGGG GGGAGGCCAG GGGAAACCGG GGGCTGCTGG GGGGGACCGA
    GGGAGCCCCT CAGAGGAGGA GGGGGCTTCC CTGAAGGCCC CATGGGCAGG GGCTCCTCTT
    CACCTGGGCC AAGGCCAGTT CCTGAAGTTC AGTCAGAGAG AAGGTGATGG AGACTCCTGG
    TCCTCAGGGG AGGACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024845075.1
    CDS162..5279
    Translation

    Target ORF information:

    RefSeq Version XM_024989307.1
    Organism Bos taurus(cattle)
    Definition Bos taurus nestin (NES), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024989307.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGAGGGCT GCCTGGGGGA GGAATCTTTT CAGATGTGGG AGCTCAACCG GCGCCTGGAG 
    GCCTACCTGG CCCGGGTCAA GGCGCTAGAA GAGCAGAATG AGCTGCTCAG CGCGGAGCTC
    GGGGGTCTCC GGGCACAGGC CGGGGACACC TCCTGGAGGG CTCGTGCCGA CGACGAGCTG
    GCGGCCCTAC GGGCCCTCGT CGACCAGCGC TGGCGGGAGA AGCACGCGGC CGAGGTGGCG
    CGCGACAACC TGGCAGAAGA GGTGGAGGGC GTGGCGAGTC GGTGCCAGCA GCTGCGGCTG
    GCCCGGGAGC GGACCGCGGA GGAGGTGGCC CGCAGCCGGC GTGCGGTCGA GGCCGAGAAA
    TGCGCCCAGG CCTGGCTGAG CACCCAGGCT GCGGAGCTGG AGCGCGAGCT GGAGGCTCTG
    CGCGTGGCGC AAGAGGAGGA GCGCGCCGGC CTGAACGCGC AGGCTGCCTG CGCCCCCCGC
    GGCTCCGCTC CGCCCCGGGG TCCCCCCGCG CCAGCCCCGG AGGTGGAGGA GCTGGCTCAG
    CGGCTGGGCG AGGCGTGGCG TGGGGCAGTG CGCGGCTACC AGGAACGCGT GGCGCACATG
    GAGACGTCGC TGGGCCAGGC CCGCGAGCGG CTAGGCCACG CGATGCAGGG CGCCCGCGAG
    GGTCGCCTGG AGCTGCAGCA ACTCCAGGCA GAGCGCAGCG GCCTTCAGGA GCGCAGGGCC
    GCGCTGGAGC AGAGGCTGGA GGGCCGCTGG CAGGAGCGGC TGCGGACCAC TGAGCAGTTC
    CAGCTGGCCG TGGAGGCCCT GGAGCAGGAG AAACAAGGCC TCCAGAGCCA AATCGCCCAG
    GTCCTGGAAG GTCGGCAGCA GCTGGCACAC CTCAAGATGT CCCTCAGCCT GGAGGTGGCC
    ACATACAGGA CCCTCCTAGA GGCTGAGAAC TCACGGCTGC AGACACCTGC AAGCGGTTCC
    AAGGCTTCCC TCAGCTTTCT GGACCCTAAG CTGGAGCTAC ATTTCCCTGG GACCCCAGAG
    GGCCGGCGTC CGGGACCTCT GCTTTCTGTC CTGAGCCCTA CTCCCCTCTC CTCACCTTTG
    CCTGGTACCC TTGAAACACC TGTGCCAACC TTTCTGAAGA GCCAGGAATT CCTTCAGGCT
    CGCACCCCGA CCTCAGCCAG CACCCCCATC CCACCCACCC CTCAGGCTCC CTGCCCTGCT
    GTAGATGCCG AGATCAGAGC CCAGGATGCC CCTGTGTCTC TGCCCCAAGC ACGAGTTGGG
    AGGCAACAGG TGCCAGAAGT CATGTGGGCT GAAGCCAAGG TGGCCATCCC CGCCAGCGTC
    CTGCCAGGAC CAGAGGAGCC TGGGGGCCAG CAACAAGAGC CCAGTCCAAG CCAGTCCCCT
    GAAGATCATG CCTCCCTGGC TCCAGCCCTC AGCCCTGACC ACTCCAGTCT AGAGACCAAA
    GATGGAGAAC CCAGTGCATC TAGAGAGTCC AGCAGATCCC AGGAGGAAGA TGAAGGCCAA
    CTGTGGGGGC TGACAGAGAA AGAAACAGTG GTAGAGGCCA AAGCAGTGAG CAGCTTGCAG
    CAGGAAACGT GTCAAGAAGA GGCGGGTCTG GACGTGAAAG AAATCCAAGA TGCCCAGGGC
    CCTTTGGAAA AAGAAGCTCT GAACTCTCTG GAAGAAGAGA TTCAAAAGCC ATCGATTCCA
    CTGGAAAAAC AGAGCCATGA GACTATAAGA TCTCTAGAGA AGGAGAATCT GGAATCTCTG
    AGGTCTTTGG AAGAAGAGAA CTTAGAAACA CTAAAAATAC TAGAAAAGGA GAATCAAGAA
    TTATTGAAGT CTTTAGAAGG AAAGGAAACG GAGGTAATGA GATCTCTAGA AAAAGAAACT
    CTAGAACTAC TTAAGCCTAT AGGAAAAGAG GATCCACAGA CATTGCCATC TCTAGGAAAG
    GAGAATCAAG AAATAATGAG GTCTCTTGAA GTTAATGTAG AGACATTCTT ATATCCAGGA
    AAGGAAAATC AAGAATTAGT GAGGTCTTTA GAAGAGGAGA ACGCTGAGTC ATTGAGAACT
    CTAGAAAAGG AGAGTCAAGA GCCACTGAGA TGTCAAGAAG TAGAGAACCA GGGAACACTG
    AGACTCCTAG CCAAAGACAA TCAGGAGCCA CTGAGGTCTC TAGAAGAAGA AGACCAGGAG
    ACGTCAAGAC CGCTAGGGAA AGAGAATCAT GAATCCCTGC GGGCTCCAGA AGATGAGAAT
    CATGAGGCTT TGAGACCTCT AGAAAGAGAC AAACCAGAGT CCCTGAGTTC TTTAGAAGAA
    GACCAGGAGG CAGTGAGACC TCCAGAAAGA GAAGACCAGG AGCTGCTGAG GTCTCTAGAA
    AAAGAAGACC AGGAGCCACT GAGGTCTCTA GAAGAAGACC AGGAGGCAGT GAGACCTCTA
    GAAGAAGAAA ACCAGGAGCT GCTGAGGTCT CTAGAAAAAG AAAAAGAGGA GTCACTGAGG
    TCTCTAGAAG ACCAGGAGGC AATGAGACCT CTAGAAGAAG AAAACCAGGA GCCGCTGAGG
    TCTCTAGAAA AAGAAAAACA GGAGGTGATG AGACCTCCAG AAGAAGAAAA ACAGGAATTA
    CTGAGATCTC TAGAAGACCA GGAGGCAATG AGATCTCTAG AAGAAGAAGG CCAGATGACA
    TTGAGCCCTC TAGAAAAAGT GAAACCAGAG ACACTAAAGT CTCTTGGAAA AGATCAGGAG
    ATAGTTAGAC CTCTTGAAAA AGAGAATCAA GAGTTATTGA GGTCCCTAAA TGAAAAGAGT
    ACAGAGGCAG TGAGGTCTTT AGAAACAGAG ACTCCAGAAC CACTAAAGCC TACAGAAGAG
    GAAAACCTGG AAATACTGAA ACCTCTAGAA AAGGAAAGTC AAGAGCCACT GGGGTCTGTG
    GAAGGGAACC ATGAGACACT GAGACCCCCA GAGAAGGAGA ATCAAGAGTC ACTGGGCTCT
    CTGGCAGAGT GGAACGTGGA GAATTTGCAG TCTCTAGAGG AGGCCGACAA GGGAAGTCTA
    AGGCACTTGG AAGAGGAAGA GGACGTGGAG CAGGAAGAGA GTCAGGCGTC ACTGAGGCCC
    CTGGAGGAGC AGGGGCAGGA GCTGCCACTC TCTGCACAGC AGCAGAAGTG GGGAGATGCA
    ATGCAGGGGG ACCAAGAACT GGATCAGGAG CGGCCTCCGG GGAGGGCTGG AGTGGACAGT
    GGGGATGGGG CAGAGCTGGA ACTGAAAGAA CTGGATAGCT CCCCTGCGGA GGGGGAGGTG
    GTGGAGCAGG GTCAGCTGCA GCTGACAGCC ACAGGTGAGG CCTGGGGCCC AGGTGAGGGG
    CAGCCAGGCA GCCCTGAGCC CAAAGAGCAG AGGCTCCCAG CTGAGGGTGC CGGAGGGGCA
    GGAGGCGCTG CGGGCCTCCA GGACCCTGAG GAGCAGCCAG AGCAGGTGGG GGCCCTGGGC
    CTCCCGGCTG CCCAGGGAAC ATCAGAGGTG ATGGAGCCCG AGTTGGAAGA TGAGGATGTG
    GCCCCAGGGG GTGGCCGAGC CTCCCCAGAG GTCACCTTGG AGTTAGAGAG GGCCATGGGC
    AGGTCTGAGG GAGTGGAACG GGGACCCGAG CAGGAGGCAG TAAAGCTGGA GGACCCAGGT
    GGCCTGGCCA GAGAGGAGGT GATGGAGCCA CCCCTGGGGG AGGGAGGTAT GGCAGCAAAG
    AAGGTACAGG GCTTGGAAGG GCCCAGAAAG GAGCTGGAGG AGGCAGGCAC TCTGGAGCCT
    GAGTCCGGGA AGAGCAGAGA CCCGTTGGAG ACTCCTAGGG ACTGGGAGGA GTCAGAATCT
    GGGGCCCTTG GGAAAACAGA GGAGCCAGTC TCAGCTGAGA CCTTGTGGTC TCACGAGGGA
    AGTGATACCC TCCAGCCCAG GCCCCTGGGG TCAGAGGGAG CTGAGGAGGA TGCCAAACCA
    CTGCTGGGGC CCCCCAGTCT GAGGCCCACT GAGTCCTGCT TGCCCAGCCT AATCCCTGAA
    GATGCCCCTG GGCCCCAGCC CCTGGCTGAG GGGAACCAAG AAGCCAGCTG GGGGCTGGAG
    GGCAGGGCTG AGGTCGTAGG AAAGGCAGGA GGTGAGCAGG AGGATCTGGG CTCTGGGGGG
    ATCCCTGAGG GCCTCCAGGA GGAAGGGGAG GAGAGCAGAG AAGAGAGCGA GGCGGATGAG
    CTAGGGGAGA CCCTTCCTGA CTCCACTCCC CTAGGCCTCT ACCTCAGGTC CCCCGCTTCC
    CCCAAGTGGG ACCTGCCTGG AGAGCAGAGG CCCTCCCCTC AAGGGGAGCC CAGAAAAGAA
    GGCTGGGATC CTGTGGTCCC AGCCTCCAAG AGCCTTGGGG CCCAGCCCTC AGAGGAGGAA
    GAAGAGGAGG GAGCTGAGGA GGAGGAACGT GGCCATGATT CTGAGCTGTC GGAAGAGTTT
    GAGGACCTGG GGACTGAGGC TTCTCTCCTT CCCGGGGTCC CGGGGGAGGG GGAAGAACCT
    CTGAGCCAAG TGCCCCAGCT GCTCCTGGAG CCTGCAGCCT GGGATCGTGA TGGTGAATCT
    GACGGATTTG CAGATGAGGA AGAGAGCGGG GAGGAGGGCG AGGAAGAAGA TGAAGAAGAG
    GGGAGGGAGC CAGGGCCAGG GCATTGGGGG CCAGCGCCCT CTGTGGGCAG CCTCCCCGCC
    CTGAGCGGCC CTCAGGGAGG GCACCTCCTG GAGTCTGAGA CCGTGGGTGT CAGTGTCCCC
    TGGGACGATG GCCCAAGGGG CGCGGCAGCC GACGCCCCCG TGACTGCCCT GGAGACCGAG
    TCCCAGAACA GCACTGAGGC CTCAGGCTCA GAGGAAGAGT CTGATGCTGC TCCCCTGGAG
    ACGGAGGACC AGGTCCCTGG CCCTCTGGGG ACCCTCAGCG GGGTGGAGGA CACCCTTGAT
    GGTGGCCCAG GCCCCAGCCT GAAGGAAGAG CTGGAGCGCA TGAATGGGGG CGTGGTGAAC
    GGGCTGGAGC AGTCCGAGGG GGGAGGCCAG GGGAAACCGG GGGCTGCTGG GGGGGACCGA
    GGGAGCCCCT CAGAGGAGGA GGGGGCTTCC CTGAAGGCCC CATGGGCAGG GGCTCCTCTT
    CACCTGGGCC AAGGCCAGTT CCTGAAGTTC AGTCAGAGAG AAGGTGATGG AGACTCCTGG
    TCCTCAGGGG AGGACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa37137
    Clone ID Related Accession (Same CDS sequence) NM_001206591.1
    Accession Version NM_001206591.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5397bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-29
    Organism Bos taurus(cattle)
    Product nestin
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from NKLS02000003.1. On or before May 4, 2011 this sequence version replaced XM_002686008.1, XM_600663.4. Sequence Note: This RefSeq record was created from genomic sequence data. The genomic coordinates used for the transcript record were predicted based on cow transcript alignments and on homologous protein alignment with human NP_006608.1 and mouse NP_057910.3. ##Evidence-Data-START## Transcript exon combination :: SRR5571258.193.1, DN540890.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145414 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGGAGGGCT GCCTGGGGGA GGAATCTTTT CAGATGTGGG AGCTCAACCG GCGCCTGGAG 
    GCCTACCTGG CCCGGGTCAA GGCGCTAGAA GAGCAGAATG AGCTGCTCAG CGCGGAGCTC
    GGGGGTCTCC GGGCACAGGC CGGGGACACC TCCTGGAGGG CTCGTGCCGA CGACGAGCTG
    GCGGCCCTAC GGGCCCTCGT CGACCAGCGC TGGCGGGAGA AGCACGCGGC CGAGGTGGCG
    CGCGACAACC TGGCAGAAGA GGTGGAGGGC GTGGCGAGTC GGTGCCAGCA GCTGCGGCTG
    GCCCGGGAGC GGACCGCGGA GGAGGTGGCC CGCAGCCGGC GTGCGGTCGA GGCCGAGAAA
    TGCGCCCAGG CCTGGCTGAG CACCCAGGCT GCGGAGCTGG AGCGCGAGCT GGAGGCTCTG
    CGCGTGGCGC AAGAGGAGGA GCGCGCCGGC CTGAACGCGC AGGCTGCCTG CGCCCCCCGC
    GGCTCCGCTC CGCCCCGGGG TCCCCCCGCG CCAGCCCCGG AGGTGGAGGA GCTGGCTCAG
    CGGCTGGGCG AGGCGTGGCG TGGGGCAGTG CGCGGCTACC AGGAACGCGT GGCGCACATG
    GAGACGTCGC TGGGCCAGGC CCGCGAGCGG CTAGGCCACG CGATGCAGGG CGCCCGCGAG
    GGTCGCCTGG AGCTGCAGCA ACTCCAGGCA GAGCGCAGCG GCCTTCAGGA GCGCAGGGCC
    GCGCTGGAGC AGAGGCTGGA GGGCCGCTGG CAGGAGCGGC TGCGGACCAC TGAGCAGTTC
    CAGCTGGCCG TGGAGGCCCT GGAGCAGGAG AAACAAGGCC TCCAGAGCCA AATCGCCCAG
    GTCCTGGAAG GTCGGCAGCA GCTGGCACAC CTCAAGATGT CCCTCAGCCT GGAGGTGGCC
    ACATACAGGA CCCTCCTAGA GGCTGAGAAC TCACGGCTGC AGACACCTGC AAGCGGTTCC
    AAGGCTTCCC TCAGCTTTCT GGACCCTAAG CTGGAGCTAC ATTTCCCTGG GACCCCAGAG
    GGCCGGCGTC CGGGACCTCT GCTTTCTGTC CTGAGCCCTA CTCCCCTCTC CTCACCTTTG
    CCTGGTACCC TTGAAACACC TGTGCCAACC TTTCTGAAGA GCCAGGAATT CCTTCAGGCT
    CGCACCCCGA CCTCAGCCAG CACCCCCATC CCACCCACCC CTCAGGCTCC CTGCCCTGCT
    GTAGATGCCG AGATCAGAGC CCAGGATGCC CCTGTGTCTC TGCCCCAAGC ACGAGTTGGG
    AGGCAACAGG TGCCAGAAGT CATGTGGGCT GAAGCCAAGG TGGCCATCCC CGCCAGCGTC
    CTGCCAGGAC CAGAGGAGCC TGGGGGCCAG CAACAAGAGC CCAGTCCAAG CCAGTCCCCT
    GAAGATCATG CCTCCCTGGC TCCAGCCCTC AGCCCTGACC ACTCCAGTCT AGAGACCAAA
    GATGGAGAAC CCAGTGCATC TAGAGAGTCC AGCAGATCCC AGGAGGAAGA TGAAGGCCAA
    CTGTGGGGGC TGACAGAGAA AGAAACAGTG GTAGAGGCCA AAGCAGTGAG CAGCTTGCAG
    CAGGAAACGT GTCAAGAAGA GGCGGGTCTG GACGTGAAAG AAATCCAAGA TGCCCAGGGC
    CCTTTGGAAA AAGAAGCTCT GAACTCTCTG GAAGAAGAGA TTCAAAAGCC ATCGATTCCA
    CTGGAAAAAC AGAGCCATGA GACTATAAGA TCTCTAGAGA AGGAGAATCT GGAATCTCTG
    AGGTCTTTGG AAGAAGAGAA CTTAGAAACA CTAAAAATAC TAGAAAAGGA GAATCAAGAA
    TTATTGAAGT CTTTAGAAGG AAAGGAAACG GAGGTAATGA GATCTCTAGA AAAAGAAACT
    CTAGAACTAC TTAAGCCTAT AGGAAAAGAG GATCCACAGA CATTGCCATC TCTAGGAAAG
    GAGAATCAAG AAATAATGAG GTCTCTTGAA GTTAATGTAG AGACATTCTT ATATCCAGGA
    AAGGAAAATC AAGAATTAGT GAGGTCTTTA GAAGAGGAGA ACGCTGAGTC ATTGAGAACT
    CTAGAAAAGG AGAGTCAAGA GCCACTGAGA TGTCAAGAAG TAGAGAACCA GGGAACACTG
    AGACTCCTAG CCAAAGACAA TCAGGAGCCA CTGAGGTCTC TAGAAGAAGA AGACCAGGAG
    ACGTCAAGAC CGCTAGGGAA AGAGAATCAT GAATCCCTGC GGGCTCCAGA AGATGAGAAT
    CATGAGGCTT TGAGACCTCT AGAAAGAGAC AAACCAGAGT CCCTGAGTTC TTTAGAAGAA
    GACCAGGAGG CAGTGAGACC TCCAGAAAGA GAAGACCAGG AGCTGCTGAG GTCTCTAGAA
    AAAGAAGACC AGGAGCCACT GAGGTCTCTA GAAGAAGACC AGGAGGCAGT GAGACCTCTA
    GAAGAAGAAA ACCAGGAGCT GCTGAGGTCT CTAGAAAAAG AAAAAGAGGA GTCACTGAGG
    TCTCTAGAAG ACCAGGAGGC AATGAGACCT CTAGAAGAAG AAAACCAGGA GCCGCTGAGG
    TCTCTAGAAG ACCAGGAGGC AATGAGACCT CTAGAAGAAG AAAACCAGGA GCCGCTGAGG
    TCTCTAGAAA AAGAAAAACA GGAGGCGACG AGACCTCCAG AAGAAGAAAA ACAGGAGCCA
    CTGATGTCTC TAGAAGAAGA CCAGGAGGAA ATGAGAGCTT TAGAAGAAGA CCAGGAGACA
    GTGAGACCTC TAGAAAAAGA AAAACAGGAG TCACTAAGGT CTCTAGAAGA CCACGAGGCA
    ATGAGACCTC TAGAAGAAGA AAACCAGGAG CCGCTGAGGT CTCTAGAAAA AGAAAAACAG
    GAGGTGATGA GACCTCCAGA AGAAGAAAAA CAGGAATTAC TGAGATCTCT AGAAGACCAG
    GAGGCAATGA GATCTCTAGA AGAAGAAGGC CAGATGACAT TGAGCCCTCT AGAAAAAGTG
    AAACCAGAGA CACTAAAGTC TCTTGGAAAA GATCAGGAGA TAGTTAGACC TCTTGAAAAA
    GAGAATCAAG AGTTATTGAG GTCCCTAAAT GAAAAGAGTA CAGAGGCAGT GAGGTCTTTA
    GAAACAGAGA CTCCAGAACC ACTAAAGCCT ACAGAAGAGG AAAACCTGGA AATACTGAAA
    CCTCTAGAAA AGGAAAGTCA AGAGCCACTG GGGTCTGTGG AAGGGAACCA TGAGACACTG
    AGACCCCCAG AGAAGGAGAA TCAAGAGTCA CTGGGCTCTC TGGCAGAGTG GAACGTGGAG
    AATTTGCAGT CTCTAGAGGA GGCCGACAAG GGAAGTCTAA GGCACTTGGA AGAGGAAGAG
    GACGTGGAGC AGGAAGAGAG TCAGGCGTCA CTGAGGCCCC TGGAGGAGCA GGGGCAGGAG
    CTGCCACTCT CTGCACAGCA GCAGAAGTGG GGAGATGCAA TGCAGGGGGA CCAAGAACTG
    GATCAGGAGC GGCCTCCGGG GAGGGCTGGA GTGGACAGTG GGGATGGGGC AGAGCTGGAA
    CTGAAAGAAC TGGATAGCTC CCCTGCGGAG GGGGAGGTGG TGGAGCAGGG TCAGCTGCAG
    CTGACAGCCA CAGGTGAGGC CTGGGGCCCA GGTGAGGGGC AGCCAGGCAG CCCTGAGCCC
    AAAGAGCAGA GGCTCCCAGC TGAGGGTGCC GGAGGGGCAG GAGGCGCTGC GGGCCTCCAG
    GACCCTGAGG AGCAGCCAGA GCAGGTGGGG GCCCTGGGCC TCCCGGCTGC CCAGGGAACA
    TCAGAGGTGA TGGAGCCCGA GTTGGAAGAT GAGGATGTGG CCCCAGGGGG TGGCCGAGCC
    TCCCCAGAGG TCACCTTGGA GTTAGAGAGG GCCATGGGCA GGTCTGAGGG AGTGGAACGG
    GGACCCGAGC AGGAGGCAGT AAAGCTGGAG GACCCAGGTG GCCTGGCCAG AGAGGAGGTG
    ATGGAGCCAC CCCTGGGGGA GGGAGGTATG GCAGCAAAGA AGGTACAGGG CTTGGAAGGG
    CCCAGAAAGG AGCTGGAGGA GGCAGGCACT CTGGAGCCTG AGTCCGGGAA GAGCAGAGAC
    CCGTTGGAGA CTCCTAGGGA CTGGGAGGAG TCAGAATCTG GGGCCCTTGG GAAAACAGAG
    GAGCCAGTCT CAGCTGAGAC CTTGTGGTCT CACGAGGGAA GTGATACCCT CCAGCCCAGG
    CCCCTGGGGT CAGAGGGAGC TGAGGAGGAT GCCAAACCAC TGCTGGGGCC CCCCAGTCTG
    AGGCCCACTG AGTCCTGCTT GCCCAGCCTA ATCCCTGAAG ATGCCCCTGG GCCCCAGCCC
    CTGGCTGAGG GGAACCAAGA AGCCAGCTGG GGGCTGGAGG GCAGGGCTGA GGTCGTAGGA
    AAGGCAGGAG GTGAGCAGGA GGATCTGGGC TCTGGGGGGA TCCCTGAGGG CCTCCAGGAG
    GAAGGGGAGG AGAGCAGAGA AGAGAGCGAG GCGGATGAGC TAGGGGAGAC CCTTCCTGAC
    TCCACTCCCC TAGGCCTCTA CCTCAGGTCC CCCGCTTCCC CCAAGTGGGA CCTGCCTGGA
    GAGCAGAGGC CCTCCCCTCA AGGGGAGCCC AGAAAAGAAG GCTGGGATCC TGTGGTCCCA
    GCCTCCAAGA GCCTTGGGGC CCAGCCCTCA GAGGAGGAAG AAGAGGAGGG AGCTGAGGAG
    GAGGAACGTG GCCATGATTC TGAGCTGTCG GAAGAGTTTG AGGACCTGGG GACTGAGGCT
    TCTCTCCTTC CCGGGGTCCC GGGGGAGGGG GAAGAACCTC TGAGCCAAGT GCCCCAGCTG
    CTCCTGGAGC CTGCAGCCTG GGATCGTGAT GGTGAATCTG ACGGATTTGC AGATGAGGAA
    GAGAGCGGGG AGGAGGGCGA GGAAGAAGAT GAAGAAGAGG GGAGGGAGCC AGGGCCAGGG
    CATTGGGGGC CAGCGCCCTC TGTGGGCAGC CTCCCCGCCC TGAGCGGCCC TCAGGGAGGG
    CACCTCCTGG AGTCTGAGAC CGTGGGTGTC AGTGTCCCCT GGGACGATGG CCCAAGGGGC
    GCGGCAGCCG ACGCCCCCGT GACTGCCCTG GAGACCGAGT CCCAGAACAG CACTGAGGCC
    TCAGGCTCAG AGGAAGAGTC TGATGCTGCT CCCCTGGAGA CGGAGGACCA GGTCCCTGGC
    CCTCTGGGGA CCCTCAGCGG GGTGGAGGAC ACCCTTGATG GTGGCCCAGG CCCCAGCCTG
    AAGGAAGAGC TGGAGCGCAT GAATGGGGGC GTGGTGAACG GGCTGGAGCA GTCCGAGGGG
    GGAGGCCAGG GGAAACCGGG GGCTGCTGGG GGGGACCGAG GGAGCCCCTC AGAGGAGGAG
    GGGGCTTCCC TGAAGGCCCC ATGGGCAGGG GCTCCTCTTC ACCTGGGCCA AGGCCAGTTC
    CTGAAGTTCA GTCAGAGAGA AGGTGATGGA GACTCCTGGT CCTCAGGGGA GGACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001193520.1
    CDS1..5397
    Translation

    Target ORF information:

    RefSeq Version NM_001206591.1
    Organism Bos taurus(cattle)
    Definition Bos taurus nestin (NES), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001206591.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGGAGGGCT GCCTGGGGGA GGAATCTTTT CAGATGTGGG AGCTCAACCG GCGCCTGGAG 
    GCCTACCTGG CCCGGGTCAA GGCGCTAGAA GAGCAGAATG AGCTGCTCAG CGCGGAGCTC
    GGGGGTCTCC GGGCACAGGC CGGGGACACC TCCTGGAGGG CTCGTGCCGA CGACGAGCTG
    GCGGCCCTAC GGGCCCTCGT CGACCAGCGC TGGCGGGAGA AGCACGCGGC CGAGGTGGCG
    CGCGACAACC TGGCAGAAGA GGTGGAGGGC GTGGCGAGTC GGTGCCAGCA GCTGCGGCTG
    GCCCGGGAGC GGACCGCGGA GGAGGTGGCC CGCAGCCGGC GTGCGGTCGA GGCCGAGAAA
    TGCGCCCAGG CCTGGCTGAG CACCCAGGCT GCGGAGCTGG AGCGCGAGCT GGAGGCTCTG
    CGCGTGGCGC AAGAGGAGGA GCGCGCCGGC CTGAACGCGC AGGCTGCCTG CGCCCCCCGC
    GGCTCCGCTC CGCCCCGGGG TCCCCCCGCG CCAGCCCCGG AGGTGGAGGA GCTGGCTCAG
    CGGCTGGGCG AGGCGTGGCG TGGGGCAGTG CGCGGCTACC AGGAACGCGT GGCGCACATG
    GAGACGTCGC TGGGCCAGGC CCGCGAGCGG CTAGGCCACG CGATGCAGGG CGCCCGCGAG
    GGTCGCCTGG AGCTGCAGCA ACTCCAGGCA GAGCGCAGCG GCCTTCAGGA GCGCAGGGCC
    GCGCTGGAGC AGAGGCTGGA GGGCCGCTGG CAGGAGCGGC TGCGGACCAC TGAGCAGTTC
    CAGCTGGCCG TGGAGGCCCT GGAGCAGGAG AAACAAGGCC TCCAGAGCCA AATCGCCCAG
    GTCCTGGAAG GTCGGCAGCA GCTGGCACAC CTCAAGATGT CCCTCAGCCT GGAGGTGGCC
    ACATACAGGA CCCTCCTAGA GGCTGAGAAC TCACGGCTGC AGACACCTGC AAGCGGTTCC
    AAGGCTTCCC TCAGCTTTCT GGACCCTAAG CTGGAGCTAC ATTTCCCTGG GACCCCAGAG
    GGCCGGCGTC CGGGACCTCT GCTTTCTGTC CTGAGCCCTA CTCCCCTCTC CTCACCTTTG
    CCTGGTACCC TTGAAACACC TGTGCCAACC TTTCTGAAGA GCCAGGAATT CCTTCAGGCT
    CGCACCCCGA CCTCAGCCAG CACCCCCATC CCACCCACCC CTCAGGCTCC CTGCCCTGCT
    GTAGATGCCG AGATCAGAGC CCAGGATGCC CCTGTGTCTC TGCCCCAAGC ACGAGTTGGG
    AGGCAACAGG TGCCAGAAGT CATGTGGGCT GAAGCCAAGG TGGCCATCCC CGCCAGCGTC
    CTGCCAGGAC CAGAGGAGCC TGGGGGCCAG CAACAAGAGC CCAGTCCAAG CCAGTCCCCT
    GAAGATCATG CCTCCCTGGC TCCAGCCCTC AGCCCTGACC ACTCCAGTCT AGAGACCAAA
    GATGGAGAAC CCAGTGCATC TAGAGAGTCC AGCAGATCCC AGGAGGAAGA TGAAGGCCAA
    CTGTGGGGGC TGACAGAGAA AGAAACAGTG GTAGAGGCCA AAGCAGTGAG CAGCTTGCAG
    CAGGAAACGT GTCAAGAAGA GGCGGGTCTG GACGTGAAAG AAATCCAAGA TGCCCAGGGC
    CCTTTGGAAA AAGAAGCTCT GAACTCTCTG GAAGAAGAGA TTCAAAAGCC ATCGATTCCA
    CTGGAAAAAC AGAGCCATGA GACTATAAGA TCTCTAGAGA AGGAGAATCT GGAATCTCTG
    AGGTCTTTGG AAGAAGAGAA CTTAGAAACA CTAAAAATAC TAGAAAAGGA GAATCAAGAA
    TTATTGAAGT CTTTAGAAGG AAAGGAAACG GAGGTAATGA GATCTCTAGA AAAAGAAACT
    CTAGAACTAC TTAAGCCTAT AGGAAAAGAG GATCCACAGA CATTGCCATC TCTAGGAAAG
    GAGAATCAAG AAATAATGAG GTCTCTTGAA GTTAATGTAG AGACATTCTT ATATCCAGGA
    AAGGAAAATC AAGAATTAGT GAGGTCTTTA GAAGAGGAGA ACGCTGAGTC ATTGAGAACT
    CTAGAAAAGG AGAGTCAAGA GCCACTGAGA TGTCAAGAAG TAGAGAACCA GGGAACACTG
    AGACTCCTAG CCAAAGACAA TCAGGAGCCA CTGAGGTCTC TAGAAGAAGA AGACCAGGAG
    ACGTCAAGAC CGCTAGGGAA AGAGAATCAT GAATCCCTGC GGGCTCCAGA AGATGAGAAT
    CATGAGGCTT TGAGACCTCT AGAAAGAGAC AAACCAGAGT CCCTGAGTTC TTTAGAAGAA
    GACCAGGAGG CAGTGAGACC TCCAGAAAGA GAAGACCAGG AGCTGCTGAG GTCTCTAGAA
    AAAGAAGACC AGGAGCCACT GAGGTCTCTA GAAGAAGACC AGGAGGCAGT GAGACCTCTA
    GAAGAAGAAA ACCAGGAGCT GCTGAGGTCT CTAGAAAAAG AAAAAGAGGA GTCACTGAGG
    TCTCTAGAAG ACCAGGAGGC AATGAGACCT CTAGAAGAAG AAAACCAGGA GCCGCTGAGG
    TCTCTAGAAG ACCAGGAGGC AATGAGACCT CTAGAAGAAG AAAACCAGGA GCCGCTGAGG
    TCTCTAGAAA AAGAAAAACA GGAGGCGACG AGACCTCCAG AAGAAGAAAA ACAGGAGCCA
    CTGATGTCTC TAGAAGAAGA CCAGGAGGAA ATGAGAGCTT TAGAAGAAGA CCAGGAGACA
    GTGAGACCTC TAGAAAAAGA AAAACAGGAG TCACTAAGGT CTCTAGAAGA CCACGAGGCA
    ATGAGACCTC TAGAAGAAGA AAACCAGGAG CCGCTGAGGT CTCTAGAAAA AGAAAAACAG
    GAGGTGATGA GACCTCCAGA AGAAGAAAAA CAGGAATTAC TGAGATCTCT AGAAGACCAG
    GAGGCAATGA GATCTCTAGA AGAAGAAGGC CAGATGACAT TGAGCCCTCT AGAAAAAGTG
    AAACCAGAGA CACTAAAGTC TCTTGGAAAA GATCAGGAGA TAGTTAGACC TCTTGAAAAA
    GAGAATCAAG AGTTATTGAG GTCCCTAAAT GAAAAGAGTA CAGAGGCAGT GAGGTCTTTA
    GAAACAGAGA CTCCAGAACC ACTAAAGCCT ACAGAAGAGG AAAACCTGGA AATACTGAAA
    CCTCTAGAAA AGGAAAGTCA AGAGCCACTG GGGTCTGTGG AAGGGAACCA TGAGACACTG
    AGACCCCCAG AGAAGGAGAA TCAAGAGTCA CTGGGCTCTC TGGCAGAGTG GAACGTGGAG
    AATTTGCAGT CTCTAGAGGA GGCCGACAAG GGAAGTCTAA GGCACTTGGA AGAGGAAGAG
    GACGTGGAGC AGGAAGAGAG TCAGGCGTCA CTGAGGCCCC TGGAGGAGCA GGGGCAGGAG
    CTGCCACTCT CTGCACAGCA GCAGAAGTGG GGAGATGCAA TGCAGGGGGA CCAAGAACTG
    GATCAGGAGC GGCCTCCGGG GAGGGCTGGA GTGGACAGTG GGGATGGGGC AGAGCTGGAA
    CTGAAAGAAC TGGATAGCTC CCCTGCGGAG GGGGAGGTGG TGGAGCAGGG TCAGCTGCAG
    CTGACAGCCA CAGGTGAGGC CTGGGGCCCA GGTGAGGGGC AGCCAGGCAG CCCTGAGCCC
    AAAGAGCAGA GGCTCCCAGC TGAGGGTGCC GGAGGGGCAG GAGGCGCTGC GGGCCTCCAG
    GACCCTGAGG AGCAGCCAGA GCAGGTGGGG GCCCTGGGCC TCCCGGCTGC CCAGGGAACA
    TCAGAGGTGA TGGAGCCCGA GTTGGAAGAT GAGGATGTGG CCCCAGGGGG TGGCCGAGCC
    TCCCCAGAGG TCACCTTGGA GTTAGAGAGG GCCATGGGCA GGTCTGAGGG AGTGGAACGG
    GGACCCGAGC AGGAGGCAGT AAAGCTGGAG GACCCAGGTG GCCTGGCCAG AGAGGAGGTG
    ATGGAGCCAC CCCTGGGGGA GGGAGGTATG GCAGCAAAGA AGGTACAGGG CTTGGAAGGG
    CCCAGAAAGG AGCTGGAGGA GGCAGGCACT CTGGAGCCTG AGTCCGGGAA GAGCAGAGAC
    CCGTTGGAGA CTCCTAGGGA CTGGGAGGAG TCAGAATCTG GGGCCCTTGG GAAAACAGAG
    GAGCCAGTCT CAGCTGAGAC CTTGTGGTCT CACGAGGGAA GTGATACCCT CCAGCCCAGG
    CCCCTGGGGT CAGAGGGAGC TGAGGAGGAT GCCAAACCAC TGCTGGGGCC CCCCAGTCTG
    AGGCCCACTG AGTCCTGCTT GCCCAGCCTA ATCCCTGAAG ATGCCCCTGG GCCCCAGCCC
    CTGGCTGAGG GGAACCAAGA AGCCAGCTGG GGGCTGGAGG GCAGGGCTGA GGTCGTAGGA
    AAGGCAGGAG GTGAGCAGGA GGATCTGGGC TCTGGGGGGA TCCCTGAGGG CCTCCAGGAG
    GAAGGGGAGG AGAGCAGAGA AGAGAGCGAG GCGGATGAGC TAGGGGAGAC CCTTCCTGAC
    TCCACTCCCC TAGGCCTCTA CCTCAGGTCC CCCGCTTCCC CCAAGTGGGA CCTGCCTGGA
    GAGCAGAGGC CCTCCCCTCA AGGGGAGCCC AGAAAAGAAG GCTGGGATCC TGTGGTCCCA
    GCCTCCAAGA GCCTTGGGGC CCAGCCCTCA GAGGAGGAAG AAGAGGAGGG AGCTGAGGAG
    GAGGAACGTG GCCATGATTC TGAGCTGTCG GAAGAGTTTG AGGACCTGGG GACTGAGGCT
    TCTCTCCTTC CCGGGGTCCC GGGGGAGGGG GAAGAACCTC TGAGCCAAGT GCCCCAGCTG
    CTCCTGGAGC CTGCAGCCTG GGATCGTGAT GGTGAATCTG ACGGATTTGC AGATGAGGAA
    GAGAGCGGGG AGGAGGGCGA GGAAGAAGAT GAAGAAGAGG GGAGGGAGCC AGGGCCAGGG
    CATTGGGGGC CAGCGCCCTC TGTGGGCAGC CTCCCCGCCC TGAGCGGCCC TCAGGGAGGG
    CACCTCCTGG AGTCTGAGAC CGTGGGTGTC AGTGTCCCCT GGGACGATGG CCCAAGGGGC
    GCGGCAGCCG ACGCCCCCGT GACTGCCCTG GAGACCGAGT CCCAGAACAG CACTGAGGCC
    TCAGGCTCAG AGGAAGAGTC TGATGCTGCT CCCCTGGAGA CGGAGGACCA GGTCCCTGGC
    CCTCTGGGGA CCCTCAGCGG GGTGGAGGAC ACCCTTGATG GTGGCCCAGG CCCCAGCCTG
    AAGGAAGAGC TGGAGCGCAT GAATGGGGGC GTGGTGAACG GGCTGGAGCA GTCCGAGGGG
    GGAGGCCAGG GGAAACCGGG GGCTGCTGGG GGGGACCGAG GGAGCCCCTC AGAGGAGGAG
    GGGGCTTCCC TGAAGGCCCC ATGGGCAGGG GCTCCTCTTC ACCTGGGCCA AGGCCAGTTC
    CTGAAGTTCA GTCAGAGAGA AGGTGATGGA GACTCCTGGT CCTCAGGGGA GGACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.