Cyp7a1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cyp7a1
Entrez Gene ID	25428
Full Name	cytochrome P450 family 7 subfamily A member 1
Synonyms	CHAP,CYP7,CYP7S1
General protein information	Preferred Names cytochrome P450 family 7 subfamily A member 1 Names cholesterol 7-alpha-monooxygenase CYPVII cholesterol 7-alpha-hydroxylase cytochrome P450 (cholesterol hydroxylase 7 alpha) cytochrome P450, 7a1 cytochrome P450, family 7, subfamily a, polypeptide 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 5 Map Location: 5q12
Summary	catalyzes the conversion of cholesterol, NADPH, and oxygen to 7-alpha-hydroxycholesterol, NADP(+) and water; plays a role in bile acid biosynthesis and cholesterol metabolism [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_012942.2	NP_037074.1	cytochrome P450 7A1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Cyp7a1	NP_037074.1
Bos taurus (cattle)	CYP7A1	NP_001192606.1
Mus musculus (house mouse)	Cyp7a1	NP_031850.2
Homo sapiens (human)	CYP7A1	NP_000771.2
	CYP7A1	XP_001087135.1
Gallus gallus (chicken)	CYP7A1	NP_001001753.1
Danio rerio (zebrafish)	cyp7a1a	NP_957467.1
Pan troglodytes (chimpanzee)	CYP7A1	XP_519773.2
Canis lupus familiaris (dog)	CYP7A1	XP_544091.1
Xenopus tropicalis (tropical clawed frog)	LOC100488670	XP_002936154.1

Gene Ontology
Bibliography

Related articles in PubMed

Modulation of transport and metabolism of bile acids and bilirubin by chlorogenic acid against hepatotoxity and cholestasis in bile duct ligation rats: involvement of SIRT1-mediated deacetylation of FXR and PGC-1?.
Zhu L, Wang L, Cao F, Liu P, Bao H, Yan Y, Dong X, Wang D, Wang Z, Gong P
Journal of hepato-biliary-pancreatic sciences(2018 Jan)

Complex interaction between circadian rhythm and diet on bile acid homeostasis in male rats.
Eggink HM, Oosterman JE, de Goede P, de Vries EM, Foppen E, Koehorst M, Groen AK, Boelen A, Romijn JA, la Fleur SE, Soeters MR, Kalsbeek A
Chronobiology international34(10)1339-1353(2017)

A novel long noncoding RNA Lnc-HC binds hnRNPA2B1 to regulate expressions of Cyp7a1 and Abca1 in hepatocytic cholesterol metabolism.
Lan X, Yan J, Ren J, Zhong B, Li J, Li Y, Liu L, Yi J, Sun Q, Yang X, Sun J, Meng L, Zhu W, Holmdahl R, Li D, Lu S
Hepatology (Baltimore, Md.)64(1)58-72(2016 07)

Glucocorticoid treatment alters systemic bile acid homeostasis by regulating the biosynthesis and transport of bile salts.
Xiao Y, Yan W, Zhou K, Cao Y, Cai W
Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver48(7)771-9(2016 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

lnc-HC negatively regulates cholesterol metabolism within hepatocytes through physical interaction with hnRNPA2B1. By further binding to the target messenger RNA of Cyp7a1 or Abca1, the lnc-HC-hnRNPA2B1 complex decreases expressions of the two genes that are implicated in cellular cholesterol excretion.
Title: A novel long noncoding RNA Lnc-HC binds hnRNPA2B1 to regulate expressions of Cyp7a1 and Abca1 in hepatocytic cholesterol metabolism.

Administration of glucocorticoids (GCs) enhanced liver bile acid uptake by increasing the expression of the major hepatocyte basolateral bile transporter (Ntcp) in rats. The reduced expression of a bile acid synthesis rate-controlling enzyme, Cyp7a1, suggests that treatment with GCs suppressed hepatic bile acid synthesis.
Title: Glucocorticoid treatment alters systemic bile acid homeostasis by regulating the biosynthesis and transport of bile salts.

Dietary fats and glucomannan plus spirulina added to pork significantly alters Cyp7a1 expression in Zucker rats.
Title: Glucomannan and glucomannan plus spirulina added to pork significantly block dietary cholesterol effects on lipoproteinemia, arylesterase activity, and CYP7A1 expression in Zucker fa/fa rats.

Portal blood FGF15/19 levels remain unchanged while the corresponding ileal mRNA and liver CYP7A1 levels change significantly in cholesterol fed rats.
Title: FGF15/19 protein levels in the portal blood do not reflect changes in the ileal FGF15/19 or hepatic CYP7A1 mRNA levels.

Capsaicinoids consumption down-regulated the mRNA levels of HMG-CoA reductase and CYP7A1 in the cholesterol-free diet group but up-regulated the CYP7A1 level in the cholesterol-enriched diet group.
Title: Hypocholesterolemic effect of capsaicinoids in rats fed diets with or without cholesterol.

The following Cyp7a1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cyp7a1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13650	NM_012942.2 Latest version!	Rattus norvegicus cytochrome P450 family 7 subfamily A member 1 (Cyp7a1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$293.30 $419.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cyp7a1 ( NM_012942.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_037074.1 Rattus norvegicus cytochrome P450 family 7 subfamily A member 1 (Cyp7a1), mRNA.

Price & Availability↑

CloneID	ORa13650
Clone ID Related Accession (Same CDS sequence)	NM_012942.2
Accession Version	NM_012942.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1512bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-16
Organism	Rattus norvegicus(Norway rat)
Product	cytochrome P450 7A1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from J05460.1. On Aug 30, 2012 this sequence version replaced NM_012942.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: J05460.1, J05430.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906353 [ECO:0000348] ##Evidence-Data-END##

ATGATGACTA TTTCTTTGAT TTGGGGAATT GCCGTGTTGG TGAGCTGTTG CATATGGTTT 
ATTGTTGGAA TAAGGAGAAG GAAAGCTGGT GAACCTCCTT TGGAGAACGG GTTGATTCCG 
TACCTGGGCT GTGCTCTGAA ATTTGGATCT AATCCTCTTG AGTTCCTAAG AGCTAATCAA 
AGGAAGCATG GTCACGTTTT TACCTGCAAA CTGATGGGGA AATATGTCCA TTTCATCACA 
AACTCCCTGT CATACCACAA AGTCTTATGT CATGGAAAAT ATTTTGACTG GAAAAAATTT 
CATTACACTA CTTCTGCGAA GGCATTTGGA CACAGAAGCA TTGACCCAAA TGATGGAAAT 
ACCACGGAAA ATATAAACAA CACTTTTACC AAAACCCTCC AGGGAGATGC TCTGTGTTCA 
CTTTCTGAAG CCATGATGCA AAACCTCCAA TCTGTCATGA GACCTCCTGG CCTTCCTAAA 
TCAAAGAGCA ATGCCTGGGT CACGGAAGGG ATGTATGCCT TCTGTTACCG AGTGATGTTT 
GAAGCTGGCT ATCTAACACT GTTTGGCAGA GATATTTCAA AGACAGACAC ACAAAAAGCA 
CTTATTCTAA ACAACCTTGA CAACTTCAAA CAATTTGACC AAGTCTTTCC GGCACTGGTG 
GCAGGCCTTC CTATTCACTT GTTCAAGACC GCACATAAAG CTCGGGAAAA GCTGGCTGAG 
GGATTGAAGC ACAAGAACCT GTGTGTGAGG GACCAGGTCT CTGAACTGAT CCGTCTACGT 
ATGTTTCTCA ATGACACGCT CTCCACCTTT GACGACATGG AGAAGGCCAA GACGCACCTC 
GCTATTCTCT GGGCATCTCA AGCAAACACC ATTCCTGCAA CCTTTTGGAG CTTATTTCAA 
ATGATCAGGA GTCCTGAAGC AATGAAAGCA GCCTCTGAAG AAGTGAGTGG AGCTTTACAG 
AGTGCTGGCC AAGAGCTCAG CTCTGGAGGG AGTGCCATTT ACTTGGATCA AGTGCAACTG 
AATGACCTGC CGGTACTAGA CAGCATCATC AAGGAGGCTC TGAGGCTTTC CAGTGCATCC 
TTGAATATCC GCACAGCTAA GGAGGACTTC ACTCTCCATC TTGAGGACGG TTCCTATAAC 
ATCCGAAAAG ATGACATGAT AGCTCTTTAT CCACAGTTAA TGCACTTGGA TCCTGAAATC 
TACCCAGACC CTTTGACTTT CAAATATGAC CGGTACCTTG ATGAAAGCGG GAAAGCAAAG 
ACCACCTTCT ACAGTAATGG AAACAAGCTG AAGTGTTTCT ACATGCCCTT CGGATCAGGC 
GCGACAATAT GTCCTGGAAG ACTCTTTGCC GTCCAAGAAA TCAAGCAGTT TTTGATCCTG 
ATGCTCTCCT GCTTTGAACT GGAGTTTGTG GAGAGCCAAG TCAAGTGTCC CCCTCTAGAC 
CAGTCCCGGG CAGGCTTGGG AATTTTGCCA CCACTACATG ATATTGAGTT TAAATATAAA 
CTGAAACACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_037074.1
CDS	35..1546
Translation	MMTISLIWGIAVLVSCCIWFIVGIRRRKAGEPPLENGLIPYLGCALKFGSNPLEFLRANQRKHGHVFTCKLMGKYVHFITNSLSYHKVLCHGKYFDWKKFHYTTSAKAFGHRSIDPNDGNTTENINNTFTKTLQGDALCSLSEAMMQNLQSVMRPPGLPKSKSNAWVTEGMYAFCYRVMFEAGYLTLFGRDISKTDTQKALILNNLDNFKQFDQVFPALVAGLPIHLFKTAHKAREKLAEGLKHKNLCVRDQVSELIRLRMFLNDTLSTFDDMEKAKTHLAILWASQANTIPATFWSLFQMIRSPEAMKAASEEVSGALQSAGQELSSGGSAIYLDQVQLNDLPVLDSIIKEALRLSSASLNIRTAKEDFTLHLEDGSYNIRKDDMIALYPQLMHLDPEIYPDPLTFKYDRYLDESGKAKTTFYSNGNKLKCFYMPFGSGATICPGRLFAVQEIKQFLILMLSCFELEFVESQVKCPPLDQSRAGLGILPPLHDIEFKYKLKH

Target ORF information:

RefSeq Version	NM_012942.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cytochrome P450 family 7 subfamily A member 1 (Cyp7a1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_012942.2

ORF Insert Sequence:

ATGATGACTA TTTCTTTGAT TTGGGGAATT GCCGTGTTGG TGAGCTGTTG CATATGGTTT 
ATTGTTGGAA TAAGGAGAAG GAAAGCTGGT GAACCTCCTT TGGAGAACGG GTTGATTCCG 
TACCTGGGCT GTGCTCTGAA ATTTGGATCT AATCCTCTTG AGTTCCTAAG AGCTAATCAA 
AGGAAGCATG GTCACGTTTT TACCTGCAAA CTGATGGGGA AATATGTCCA TTTCATCACA 
AACTCCCTGT CATACCACAA AGTCTTATGT CATGGAAAAT ATTTTGACTG GAAAAAATTT 
CATTACACTA CTTCTGCGAA GGCATTTGGA CACAGAAGCA TTGACCCAAA TGATGGAAAT 
ACCACGGAAA ATATAAACAA CACTTTTACC AAAACCCTCC AGGGAGATGC TCTGTGTTCA 
CTTTCTGAAG CCATGATGCA AAACCTCCAA TCTGTCATGA GACCTCCTGG CCTTCCTAAA 
TCAAAGAGCA ATGCCTGGGT CACGGAAGGG ATGTATGCCT TCTGTTACCG AGTGATGTTT 
GAAGCTGGCT ATCTAACACT GTTTGGCAGA GATATTTCAA AGACAGACAC ACAAAAAGCA 
CTTATTCTAA ACAACCTTGA CAACTTCAAA CAATTTGACC AAGTCTTTCC GGCACTGGTG 
GCAGGCCTTC CTATTCACTT GTTCAAGACC GCACATAAAG CTCGGGAAAA GCTGGCTGAG 
GGATTGAAGC ACAAGAACCT GTGTGTGAGG GACCAGGTCT CTGAACTGAT CCGTCTACGT 
ATGTTTCTCA ATGACACGCT CTCCACCTTT GACGACATGG AGAAGGCCAA GACGCACCTC 
GCTATTCTCT GGGCATCTCA AGCAAACACC ATTCCTGCAA CCTTTTGGAG CTTATTTCAA 
ATGATCAGGA GTCCTGAAGC AATGAAAGCA GCCTCTGAAG AAGTGAGTGG AGCTTTACAG 
AGTGCTGGCC AAGAGCTCAG CTCTGGAGGG AGTGCCATTT ACTTGGATCA AGTGCAACTG 
AATGACCTGC CGGTACTAGA CAGCATCATC AAGGAGGCTC TGAGGCTTTC CAGTGCATCC 
TTGAATATCC GCACAGCTAA GGAGGACTTC ACTCTCCATC TTGAGGACGG TTCCTATAAC 
ATCCGAAAAG ATGACATGAT AGCTCTTTAT CCACAGTTAA TGCACTTGGA TCCTGAAATC 
TACCCAGACC CTTTGACTTT CAAATATGAC CGGTACCTTG ATGAAAGCGG GAAAGCAAAG 
ACCACCTTCT ACAGTAATGG AAACAAGCTG AAGTGTTTCT ACATGCCCTT CGGATCAGGC 
GCGACAATAT GTCCTGGAAG ACTCTTTGCC GTCCAAGAAA TCAAGCAGTT TTTGATCCTG 
ATGCTCTCCT GCTTTGAACT GGAGTTTGTG GAGAGCCAAG TCAAGTGTCC CCCTCTAGAC 
CAGTCCCGGG CAGGCTTGGG AATTTTGCCA CCACTACATG ATATTGAGTT TAAATATAAA 
CTGAAACACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Modulation of transport and metabolism of bile acids and bilirubin by chlorogenic acid against hepatotoxity and cholestasis in bile duct ligation rats: involvement of SIRT1-mediated deacetylation of FXR and PGC-1?.
Journal of hepato-biliary-pancreatic sciences(2018 Jan)
Zhu L,Wang L,Cao F,Liu P,Bao H,Yan Y,Dong X,Wang D,Wang Z,Gong P

Complex interaction between circadian rhythm and diet on bile acid homeostasis in male rats.
Chronobiology international34(10)1339-1353(2017)
Eggink HM,Oosterman JE,de Goede P,de Vries EM,Foppen E,Koehorst M,Groen AK,Boelen A,Romijn JA,la Fleur SE,Soeters MR,Kalsbeek A

A novel long noncoding RNA Lnc-HC binds hnRNPA2B1 to regulate expressions of Cyp7a1 and Abca1 in hepatocytic cholesterol metabolism.
Hepatology (Baltimore, Md.)64(1)58-72(2016 07)
Lan X,Yan J,Ren J,Zhong B,Li J,Li Y,Liu L,Yi J,Sun Q,Yang X,Sun J,Meng L,Zhu W,Holmdahl R,Li D,Lu S

Glucocorticoid treatment alters systemic bile acid homeostasis by regulating the biosynthesis and transport of bile salts.
Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver48(7)771-9(2016 Jul)
Xiao Y,Yan W,Zhou K,Cao Y,Cai W

- Most Popular Services
  - 人工遺伝子合成& DNA合成-納期保証
- Services & Products

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

Cyp7a1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報