CYP7A1 cDNA ORF clone, Homo sapiens(Human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CYP7A1
Entrez Gene ID	1581
Full Name	cytochrome P450 family 7 subfamily A member 1
Synonyms	CP7A,CYP7,CYPVII
General protein information	Preferred Names cytochrome P450 family 7 subfamily A member 1 Names cholesterol 7-alpha-monooxygenase cholesterol 7-alpha-hydroxylase cytochrome P450 7A1 cytochrome P450, family 7, subfamily A, polypeptide 1 cytochrome P450, subfamily VIIA polypeptide 1
Gene Type	protein-coding
Organism	Homo sapiens(Human)
Genome	Chromosome: 8 Map Location: 8q12.1
Summary	This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the first reaction in the cholesterol catabolic pathway in the liver, which converts cholesterol to bile acids. This reaction is the rate limiting step and the major site of regulation of bile acid synthesis, which is the primary mechanism for the removal of cholesterol from the body. Polymorphisms in the promoter of this gene are associated with defects in bile acid synthesis. [provided by RefSeq, Feb 2010].
Disorder MIM:	118455

mRNA and Protein(s)

mRNA	Protein	Name
NM_000780.3	NP_000771.2	cholesterol 7-alpha-monooxygenase
NM_000780.4	NP_000771.2	cytochrome P450 7A1
XM_011517476.1	XP_011515778.1	cholesterol 7-alpha-monooxygenase isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Cyp7a1	NP_037074.1
Bos taurus (cattle)	CYP7A1	NP_001192606.1
Mus musculus (house mouse)	Cyp7a1	NP_031850.2
Homo sapiens (human)	CYP7A1	NP_000771.2
	CYP7A1	XP_001087135.1
Gallus gallus (chicken)	CYP7A1	NP_001001753.1
Danio rerio (zebrafish)	cyp7a1a	NP_957467.1
Pan troglodytes (chimpanzee)	CYP7A1	XP_519773.2
Canis lupus familiaris (dog)	CYP7A1	XP_544091.1
Xenopus tropicalis (tropical clawed frog)	LOC100488670	XP_002936154.1

Gene Ontology
Bibliography

Related articles in PubMed

REV-ERBa Regulates CYP7A1 through Repression of Liver Receptor Homolog-1.
Zhang T, Zhao M, Lu D, Wang S, Yu F, Guo L, Wen S, Wu B
Drug metabolism and disposition: the biological fate of chemicals(2017 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The pronounced TC reduction in G allele carriers of rs3808607 observed in the previous study may be due to enhanced bile acid synthesis in response to high-viscosity beta-glucan consumption in those individuals.
Title: Barley ?-glucan reduces blood cholesterol levels via interrupting bile acid metabolism.

Current studies suggest that increased Cyp7a1 expression and bile acid synthesis ameliorate hepatic inflammation through activation of FXR
Title: Cholesterol 7?-hydroxylase protects the liver from inflammation and fibrosis by maintaining cholesterol homeostasis.

Genetic variations in ABCG5, CYP7A1, and DHCR7 may contribute to differing responses of serum cholesterol to dairy intake among healthy adults.
Title: Common Variants in Cholesterol Synthesis- and Transport-Related Genes Associate with Circulating Cholesterol Responses to Intakes of Conventional Dairy Products in Healthy Individuals.

The promoter polymorphism of the CYP7A1 gene has a pronounced impact on diurnal variation in CYP7A1 activity.
Title: Diurnal variation in cholesterol 7?-hydroxylase activity is determined by the -203A>C polymorphism of the CYP7A1 gene.

This study provides evidence that individuals carrying the G allele of CYP7A1 single nucleotide polymorphism are more responsive to high molecular weight beta-glucan's effect of lowering circulating cholesterol concentrations than individuals homozygous for the T allele.
Title: High-Molecular-Weight ?-Glucan Decreases Serum Cholesterol Differentially Based on the CYP7A1 rs3808607 Polymorphism in Mildly Hypercholesterolemic Adults.

The following CYP7A1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CYP7A1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu26274	NM_000780.4 Latest version!	Homo sapiens cytochrome P450 family 7 subfamily A member 1 (CYP7A1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu26274	XM_011517476.1 Latest version!	Homo sapiens cytochrome P450, family 7, subfamily A, polypeptide 1 (CYP7A1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
View Old Accession Versions >>
OHu26274	NM_000780.3	Homo sapiens cytochrome P450 family 7 subfamily A member 1 (CYP7A1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CYP7A1 ( NM_000780.3 ) cDNA ORF clone, Homo sapiens(Human) -> NP_000771.2 Homo sapiens cytochrome P450 family 7 subfamily A member 1 (CYP7A1), mRNA.

Price & Availability↑

CloneID	OHu26274
Clone ID Related Accession (Same CDS sequence)	NM_000780.3 , NM_000780.4 , XM_011517476.1
Accession Version	NM_000780.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1515bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-17
Organism	Homo sapiens(human)
Product	cholesterol 7-alpha-monooxygenase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC101777.1 and AC009927.10. This sequence is a reference standard in the RefSeqGene project. On Jan 31, 2008 this sequence version replaced NM_000780.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M93133.1, X56088.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145122, SAMEA2155590 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGATGACCA CATCTTTGAT TTGGGGGATT GCTATAGCAG CATGCTGTTG TCTATGGCTT 
ATTCTTGGAA TTAGGAGAAG GCAAACGGGT GAACCACCTC TAGAGAATGG ATTAATTCCA 
TACCTGGGCT GTGCTCTGCA ATTTGGTGCC AATCCTCTTG AGTTCCTCAG AGCAAATCAA 
AGGAAACATG GTCATGTTTT TACCTGCAAA CTAATGGGAA AATATGTCCA TTTCATCACA 
AATCCCTTGT CATACCATAA GGTGTTGTGC CACGGAAAAT ATTTTGATTG GAAAAAATTT 
CACTTTGCTA CTTCTGCGAA GGCATTTGGG CACAGAAGCA TTGACCCGAT GGATGGAAAT 
ACCACTGAAA ACATAAACGA CACTTTCATC AAAACCCTGC AGGGCCATGC CTTGAATTCC 
CTCACGGAAA GCATGATGGA AAACCTCCAA CGTATCATGA GACCTCCAGT CTCCTCTAAC 
TCAAAGACCG CTGCCTGGGT GACAGAAGGG ATGTATTCTT TCTGCTACCG AGTGATGTTT 
GAAGCTGGGT ATTTAACTAT CTTTGGCAGA GATCTTACAA GGCGGGACAC ACAGAAAGCA 
CATATTCTAA ACAATCTTGA CAACTTCAAG CAATTCGACA AAGTCTTTCC AGCCCTGGTA 
GCAGGCCTCC CCATTCACAT GTTCAGGACT GCGCACAATG CCCGGGAGAA ACTGGCAGAG 
AGCTTGAGGC ACGAGAACCT CCAAAAGAGG GAAAGCATCT CAGAACTGAT CAGCCTGCGC 
ATGTTTCTCA ATGACACTTT GTCCACCTTT GATGATCTGG AGAAGGCCAA GACACACCTC 
GTGGTCCTCT GGGCATCGCA AGCAAACACC ATTCCAGCGA CTTTCTGGAG TTTATTTCAA 
ATGATTAGGA ACCCAGAAGC AATGAAAGCA GCTACTGAAG AAGTGAAAAG AACATTAGAG 
AATGCTGGTC AAAAAGTCAG CTTGGAAGGC AATCCTATTT GTTTGAGTCA AGCAGAACTG 
AATGACCTGC CAGTATTAGA TAGTATAATC AAGGAATCGC TGAGGCTTTC CAGTGCCTCC 
CTCAACATCC GGACAGCTAA GGAGGATTTC ACTTTGCACC TTGAGGACGG TTCCTACAAC 
ATCCGAAAAG ATGACATCAT AGCTCTTTAC CCACAGTTAA TGCACTTAGA TCCAGAAATC 
TACCCAGACC CTTTGACTTT TAAATATGAT AGGTATCTTG ATGAAAACGG GAAGACAAAG 
ACTACCTTCT ATTGTAATGG ACTCAAGTTA AAGTATTACT ACATGCCCTT TGGATCGGGA 
GCTACAATAT GTCCTGGAAG ATTGTTCGCT ATCCACGAAA TCAAGCAATT TTTGATTCTG 
ATGCTTTCTT ATTTTGAATT GGAGCTTATA GAGGGCCAAG CTAAATGTCC ACCTTTGGAC 
CAGTCCCGGG CAGGCTTGGG CATTTTGCCG CCATTGAATG ATATTGAATT TAAATATAAA 
TTCAAGCATT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000771.2
CDS	64..1578
Translation	MMTTSLIWGIAIAACCCLWLILGIRRRQTGEPPLENGLIPYLGCALQFGANPLEFLRANQRKHGHVFTCKLMGKYVHFITNPLSYHKVLCHGKYFDWKKFHFATSAKAFGHRSIDPMDGNTTENINDTFIKTLQGHALNSLTESMMENLQRIMRPPVSSNSKTAAWVTEGMYSFCYRVMFEAGYLTIFGRDLTRRDTQKAHILNNLDNFKQFDKVFPALVAGLPIHMFRTAHNAREKLAESLRHENLQKRESISELISLRMFLNDTLSTFDDLEKAKTHLVVLWASQANTIPATFWSLFQMIRNPEAMKAATEEVKRTLENAGQKVSLEGNPICLSQAELNDLPVLDSIIKESLRLSSASLNIRTAKEDFTLHLEDGSYNIRKDDIIALYPQLMHLDPEIYPDPLTFKYDRYLDENGKTKTTFYCNGLKLKYYYMPFGSGATICPGRLFAIHEIKQFLILMLSYFELELIEGQAKCPPLDQSRAGLGILPPLNDIEFKYKFKHL

Target ORF information:

RefSeq Version	NM_000780.3
Organism	Homo sapiens(human)
Definition	Homo sapiens cytochrome P450 family 7 subfamily A member 1 (CYP7A1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000780.3

ORF Insert Sequence:

ATGATGACCA CATCTTTGAT TTGGGGGATT GCTATAGCAG CATGCTGTTG TCTATGGCTT 
ATTCTTGGAA TTAGGAGAAG GCAAACGGGT GAACCACCTC TAGAGAATGG ATTAATTCCA 
TACCTGGGCT GTGCTCTGCA ATTTGGTGCC AATCCTCTTG AGTTCCTCAG AGCAAATCAA 
AGGAAACATG GTCATGTTTT TACCTGCAAA CTAATGGGAA AATATGTCCA TTTCATCACA 
AATCCCTTGT CATACCATAA GGTGTTGTGC CACGGAAAAT ATTTTGATTG GAAAAAATTT 
CACTTTGCTA CTTCTGCGAA GGCATTTGGG CACAGAAGCA TTGACCCGAT GGATGGAAAT 
ACCACTGAAA ACATAAACGA CACTTTCATC AAAACCCTGC AGGGCCATGC CTTGAATTCC 
CTCACGGAAA GCATGATGGA AAACCTCCAA CGTATCATGA GACCTCCAGT CTCCTCTAAC 
TCAAAGACCG CTGCCTGGGT GACAGAAGGG ATGTATTCTT TCTGCTACCG AGTGATGTTT 
GAAGCTGGGT ATTTAACTAT CTTTGGCAGA GATCTTACAA GGCGGGACAC ACAGAAAGCA 
CATATTCTAA ACAATCTTGA CAACTTCAAG CAATTCGACA AAGTCTTTCC AGCCCTGGTA 
GCAGGCCTCC CCATTCACAT GTTCAGGACT GCGCACAATG CCCGGGAGAA ACTGGCAGAG 
AGCTTGAGGC ACGAGAACCT CCAAAAGAGG GAAAGCATCT CAGAACTGAT CAGCCTGCGC 
ATGTTTCTCA ATGACACTTT GTCCACCTTT GATGATCTGG AGAAGGCCAA GACACACCTC 
GTGGTCCTCT GGGCATCGCA AGCAAACACC ATTCCAGCGA CTTTCTGGAG TTTATTTCAA 
ATGATTAGGA ACCCAGAAGC AATGAAAGCA GCTACTGAAG AAGTGAAAAG AACATTAGAG 
AATGCTGGTC AAAAAGTCAG CTTGGAAGGC AATCCTATTT GTTTGAGTCA AGCAGAACTG 
AATGACCTGC CAGTATTAGA TAGTATAATC AAGGAATCGC TGAGGCTTTC CAGTGCCTCC 
CTCAACATCC GGACAGCTAA GGAGGATTTC ACTTTGCACC TTGAGGACGG TTCCTACAAC 
ATCCGAAAAG ATGACATCAT AGCTCTTTAC CCACAGTTAA TGCACTTAGA TCCAGAAATC 
TACCCAGACC CTTTGACTTT TAAATATGAT AGGTATCTTG ATGAAAACGG GAAGACAAAG 
ACTACCTTCT ATTGTAATGG ACTCAAGTTA AAGTATTACT ACATGCCCTT TGGATCGGGA 
GCTACAATAT GTCCTGGAAG ATTGTTCGCT ATCCACGAAA TCAAGCAATT TTTGATTCTG 
ATGCTTTCTT ATTTTGAATT GGAGCTTATA GAGGGCCAAG CTAAATGTCC ACCTTTGGAC 
CAGTCCCGGG CAGGCTTGGG CATTTTGCCG CCATTGAATG ATATTGAATT TAAATATAAA 
TTCAAGCATT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CYP7A1 ( NM_000780.4 ) cDNA ORF clone, Homo sapiens(Human) -> NP_000771.2 Homo sapiens cytochrome P450 family 7 subfamily A member 1 (CYP7A1), mRNA.

Price & Availability↑

CloneID	OHu26274
Clone ID Related Accession (Same CDS sequence)	NM_000780.3 , NM_000780.4 , XM_011517476.1
Accession Version	NM_000780.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1515bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-09
Organism	Homo sapiens(human)
Product	cytochrome P450 7A1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC009927.10 and BC101777.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_000780.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M93133.1, X56088.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145122, SAMEA2155590 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000301645.4/ ENSP00000301645.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGATGACCA CATCTTTGAT TTGGGGGATT GCTATAGCAG CATGCTGTTG TCTATGGCTT 
ATTCTTGGAA TTAGGAGAAG GCAAACGGGT GAACCACCTC TAGAGAATGG ATTAATTCCA 
TACCTGGGCT GTGCTCTGCA ATTTGGTGCC AATCCTCTTG AGTTCCTCAG AGCAAATCAA 
AGGAAACATG GTCATGTTTT TACCTGCAAA CTAATGGGAA AATATGTCCA TTTCATCACA 
AATCCCTTGT CATACCATAA GGTGTTGTGC CACGGAAAAT ATTTTGATTG GAAAAAATTT 
CACTTTGCTA CTTCTGCGAA GGCATTTGGG CACAGAAGCA TTGACCCGAT GGATGGAAAT 
ACCACTGAAA ACATAAACGA CACTTTCATC AAAACCCTGC AGGGCCATGC CTTGAATTCC 
CTCACGGAAA GCATGATGGA AAACCTCCAA CGTATCATGA GACCTCCAGT CTCCTCTAAC 
TCAAAGACCG CTGCCTGGGT GACAGAAGGG ATGTATTCTT TCTGCTACCG AGTGATGTTT 
GAAGCTGGGT ATTTAACTAT CTTTGGCAGA GATCTTACAA GGCGGGACAC ACAGAAAGCA 
CATATTCTAA ACAATCTTGA CAACTTCAAG CAATTCGACA AAGTCTTTCC AGCCCTGGTA 
GCAGGCCTCC CCATTCACAT GTTCAGGACT GCGCACAATG CCCGGGAGAA ACTGGCAGAG 
AGCTTGAGGC ACGAGAACCT CCAAAAGAGG GAAAGCATCT CAGAACTGAT CAGCCTGCGC 
ATGTTTCTCA ATGACACTTT GTCCACCTTT GATGATCTGG AGAAGGCCAA GACACACCTC 
GTGGTCCTCT GGGCATCGCA AGCAAACACC ATTCCAGCGA CTTTCTGGAG TTTATTTCAA 
ATGATTAGGA ACCCAGAAGC AATGAAAGCA GCTACTGAAG AAGTGAAAAG AACATTAGAG 
AATGCTGGTC AAAAAGTCAG CTTGGAAGGC AATCCTATTT GTTTGAGTCA AGCAGAACTG 
AATGACCTGC CAGTATTAGA TAGTATAATC AAGGAATCGC TGAGGCTTTC CAGTGCCTCC 
CTCAACATCC GGACAGCTAA GGAGGATTTC ACTTTGCACC TTGAGGACGG TTCCTACAAC 
ATCCGAAAAG ATGACATCAT AGCTCTTTAC CCACAGTTAA TGCACTTAGA TCCAGAAATC 
TACCCAGACC CTTTGACTTT TAAATATGAT AGGTATCTTG ATGAAAACGG GAAGACAAAG 
ACTACCTTCT ATTGTAATGG ACTCAAGTTA AAGTATTACT ACATGCCCTT TGGATCGGGA 
GCTACAATAT GTCCTGGAAG ATTGTTCGCT ATCCACGAAA TCAAGCAATT TTTGATTCTG 
ATGCTTTCTT ATTTTGAATT GGAGCTTATA GAGGGCCAAG CTAAATGTCC ACCTTTGGAC 
CAGTCCCGGG CAGGCTTGGG CATTTTGCCG CCATTGAATG ATATTGAATT TAAATATAAA 
TTCAAGCATT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000771.2
CDS	66..1580
Translation	MMTTSLIWGIAIAACCCLWLILGIRRRQTGEPPLENGLIPYLGCALQFGANPLEFLRANQRKHGHVFTCKLMGKYVHFITNPLSYHKVLCHGKYFDWKKFHFATSAKAFGHRSIDPMDGNTTENINDTFIKTLQGHALNSLTESMMENLQRIMRPPVSSNSKTAAWVTEGMYSFCYRVMFEAGYLTIFGRDLTRRDTQKAHILNNLDNFKQFDKVFPALVAGLPIHMFRTAHNAREKLAESLRHENLQKRESISELISLRMFLNDTLSTFDDLEKAKTHLVVLWASQANTIPATFWSLFQMIRNPEAMKAATEEVKRTLENAGQKVSLEGNPICLSQAELNDLPVLDSIIKESLRLSSASLNIRTAKEDFTLHLEDGSYNIRKDDIIALYPQLMHLDPEIYPDPLTFKYDRYLDENGKTKTTFYCNGLKLKYYYMPFGSGATICPGRLFAIHEIKQFLILMLSYFELELIEGQAKCPPLDQSRAGLGILPPLNDIEFKYKFKHL

Target ORF information:

RefSeq Version	NM_000780.4
Organism	Homo sapiens(human)
Definition	Homo sapiens cytochrome P450 family 7 subfamily A member 1 (CYP7A1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000780.4

ORF Insert Sequence:

ATGATGACCA CATCTTTGAT TTGGGGGATT GCTATAGCAG CATGCTGTTG TCTATGGCTT 
ATTCTTGGAA TTAGGAGAAG GCAAACGGGT GAACCACCTC TAGAGAATGG ATTAATTCCA 
TACCTGGGCT GTGCTCTGCA ATTTGGTGCC AATCCTCTTG AGTTCCTCAG AGCAAATCAA 
AGGAAACATG GTCATGTTTT TACCTGCAAA CTAATGGGAA AATATGTCCA TTTCATCACA 
AATCCCTTGT CATACCATAA GGTGTTGTGC CACGGAAAAT ATTTTGATTG GAAAAAATTT 
CACTTTGCTA CTTCTGCGAA GGCATTTGGG CACAGAAGCA TTGACCCGAT GGATGGAAAT 
ACCACTGAAA ACATAAACGA CACTTTCATC AAAACCCTGC AGGGCCATGC CTTGAATTCC 
CTCACGGAAA GCATGATGGA AAACCTCCAA CGTATCATGA GACCTCCAGT CTCCTCTAAC 
TCAAAGACCG CTGCCTGGGT GACAGAAGGG ATGTATTCTT TCTGCTACCG AGTGATGTTT 
GAAGCTGGGT ATTTAACTAT CTTTGGCAGA GATCTTACAA GGCGGGACAC ACAGAAAGCA 
CATATTCTAA ACAATCTTGA CAACTTCAAG CAATTCGACA AAGTCTTTCC AGCCCTGGTA 
GCAGGCCTCC CCATTCACAT GTTCAGGACT GCGCACAATG CCCGGGAGAA ACTGGCAGAG 
AGCTTGAGGC ACGAGAACCT CCAAAAGAGG GAAAGCATCT CAGAACTGAT CAGCCTGCGC 
ATGTTTCTCA ATGACACTTT GTCCACCTTT GATGATCTGG AGAAGGCCAA GACACACCTC 
GTGGTCCTCT GGGCATCGCA AGCAAACACC ATTCCAGCGA CTTTCTGGAG TTTATTTCAA 
ATGATTAGGA ACCCAGAAGC AATGAAAGCA GCTACTGAAG AAGTGAAAAG AACATTAGAG 
AATGCTGGTC AAAAAGTCAG CTTGGAAGGC AATCCTATTT GTTTGAGTCA AGCAGAACTG 
AATGACCTGC CAGTATTAGA TAGTATAATC AAGGAATCGC TGAGGCTTTC CAGTGCCTCC 
CTCAACATCC GGACAGCTAA GGAGGATTTC ACTTTGCACC TTGAGGACGG TTCCTACAAC 
ATCCGAAAAG ATGACATCAT AGCTCTTTAC CCACAGTTAA TGCACTTAGA TCCAGAAATC 
TACCCAGACC CTTTGACTTT TAAATATGAT AGGTATCTTG ATGAAAACGG GAAGACAAAG 
ACTACCTTCT ATTGTAATGG ACTCAAGTTA AAGTATTACT ACATGCCCTT TGGATCGGGA 
GCTACAATAT GTCCTGGAAG ATTGTTCGCT ATCCACGAAA TCAAGCAATT TTTGATTCTG 
ATGCTTTCTT ATTTTGAATT GGAGCTTATA GAGGGCCAAG CTAAATGTCC ACCTTTGGAC 
CAGTCCCGGG CAGGCTTGGG CATTTTGCCG CCATTGAATG ATATTGAATT TAAATATAAA 
TTCAAGCATT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CYP7A1 ( XM_011517476.1 ) cDNA ORF clone, Homo sapiens(Human) -> XP_011515778.1 Homo sapiens cytochrome P450, family 7, subfamily A, polypeptide 1 (CYP7A1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu26274
Clone ID Related Accession (Same CDS sequence)	NM_000780.3 , NM_000780.4 , XM_011517476.1
Accession Version	XM_011517476.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1515bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	cholesterol 7-alpha-monooxygenase isoform X1
Comment

ATGATGACCA CATCTTTGAT TTGGGGGATT GCTATAGCAG CATGCTGTTG TCTATGGCTT 
ATTCTTGGAA TTAGGAGAAG GCAAACGGGT GAACCACCTC TAGAGAATGG ATTAATTCCA 
TACCTGGGCT GTGCTCTGCA ATTTGGTGCC AATCCTCTTG AGTTCCTCAG AGCAAATCAA 
AGGAAACATG GTCATGTTTT TACCTGCAAA CTAATGGGAA AATATGTCCA TTTCATCACA 
AATCCCTTGT CATACCATAA GGTGTTGTGC CACGGAAAAT ATTTTGATTG GAAAAAATTT 
CACTTTGCTA CTTCTGCGAA GGCATTTGGG CACAGAAGCA TTGACCCGAT GGATGGAAAT 
ACCACTGAAA ACATAAACGA CACTTTCATC AAAACCCTGC AGGGCCATGC CTTGAATTCC 
CTCACGGAAA GCATGATGGA AAACCTCCAA CGTATCATGA GACCTCCAGT CTCCTCTAAC 
TCAAAGACCG CTGCCTGGGT GACAGAAGGG ATGTATTCTT TCTGCTACCG AGTGATGTTT 
GAAGCTGGGT ATTTAACTAT CTTTGGCAGA GATCTTACAA GGCGGGACAC ACAGAAAGCA 
CATATTCTAA ACAATCTTGA CAACTTCAAG CAATTCGACA AAGTCTTTCC AGCCCTGGTA 
GCAGGCCTCC CCATTCACAT GTTCAGGACT GCGCACAATG CCCGGGAGAA ACTGGCAGAG 
AGCTTGAGGC ACGAGAACCT CCAAAAGAGG GAAAGCATCT CAGAACTGAT CAGCCTGCGC 
ATGTTTCTCA ATGACACTTT GTCCACCTTT GATGATCTGG AGAAGGCCAA GACACACCTC 
GTGGTCCTCT GGGCATCGCA AGCAAACACC ATTCCAGCGA CTTTCTGGAG TTTATTTCAA 
ATGATTAGGA ACCCAGAAGC AATGAAAGCA GCTACTGAAG AAGTGAAAAG AACATTAGAG 
AATGCTGGTC AAAAAGTCAG CTTGGAAGGC AATCCTATTT GTTTGAGTCA AGCAGAACTG 
AATGACCTGC CAGTATTAGA TAGTATAATC AAGGAATCGC TGAGGCTTTC CAGTGCCTCC 
CTCAACATCC GGACAGCTAA GGAGGATTTC ACTTTGCACC TTGAGGACGG TTCCTACAAC 
ATCCGAAAAG ATGACATCAT AGCTCTTTAC CCACAGTTAA TGCACTTAGA TCCAGAAATC 
TACCCAGACC CTTTGACTTT TAAATATGAT AGGTATCTTG ATGAAAACGG GAAGACAAAG 
ACTACCTTCT ATTGTAATGG ACTCAAGTTA AAGTATTACT ACATGCCCTT TGGATCGGGA 
GCTACAATAT GTCCTGGAAG ATTGTTCGCT ATCCACGAAA TCAAGCAATT TTTGATTCTG 
ATGCTTTCTT ATTTTGAATT GGAGCTTATA GAGGGCCAAG CTAAATGTCC ACCTTTGGAC 
CAGTCCCGGG CAGGCTTGGG CATTTTGCCG CCATTGAATG ATATTGAATT TAAATATAAA 
TTCAAGCATT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_011515778.1

Translation

MMTTSLIWGIAIAACCCLWLILGIRRRQTGEPPLENGLIPYLGCALQFGANPLEFLRANQRKHGHVFTCKLMGKYVHFITNPLSYHKVLCHGKYFDWKKFHFATSAKAFGHRSIDPMDGNTTENINDTFIKTLQGHALNSLTESMMENLQRIMRPPVSSNSKTAAWVTEGMYSFCYRVMFEAGYLTIFGRDLTRRDTQKAHILNNLDNFKQFDKVFPALVAGLPIHMFRTAHNAREKLAESLRHENLQKRESISELISLRMFLNDTLSTFDDLEKAKTHLVVLWASQANTIPATFWSLFQMIRNPEAMKAATEEVKRTLENAGQKVSLEGNPICLSQAELNDLPVLDSIIKESLRLSSASLNIRTAKEDFTLHLEDGSYNIRKDDIIALYPQLMHLDPEIYPDPLTFKYDRYLDENGKTKTTFYCNGLKLKYYYMPFGSGATICPGRLFAIHEIKQFLILMLSYFELELIEGQAKCPPLDQSRAGLGILPPLNDIEFKYKFKHL

Target ORF information:

RefSeq Version	XM_011517476.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens cytochrome P450, family 7, subfamily A, polypeptide 1 (CYP7A1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011517476.1

ORF Insert Sequence:

ATGATGACCA CATCTTTGAT TTGGGGGATT GCTATAGCAG CATGCTGTTG TCTATGGCTT 
ATTCTTGGAA TTAGGAGAAG GCAAACGGGT GAACCACCTC TAGAGAATGG ATTAATTCCA 
TACCTGGGCT GTGCTCTGCA ATTTGGTGCC AATCCTCTTG AGTTCCTCAG AGCAAATCAA 
AGGAAACATG GTCATGTTTT TACCTGCAAA CTAATGGGAA AATATGTCCA TTTCATCACA 
AATCCCTTGT CATACCATAA GGTGTTGTGC CACGGAAAAT ATTTTGATTG GAAAAAATTT 
CACTTTGCTA CTTCTGCGAA GGCATTTGGG CACAGAAGCA TTGACCCGAT GGATGGAAAT 
ACCACTGAAA ACATAAACGA CACTTTCATC AAAACCCTGC AGGGCCATGC CTTGAATTCC 
CTCACGGAAA GCATGATGGA AAACCTCCAA CGTATCATGA GACCTCCAGT CTCCTCTAAC 
TCAAAGACCG CTGCCTGGGT GACAGAAGGG ATGTATTCTT TCTGCTACCG AGTGATGTTT 
GAAGCTGGGT ATTTAACTAT CTTTGGCAGA GATCTTACAA GGCGGGACAC ACAGAAAGCA 
CATATTCTAA ACAATCTTGA CAACTTCAAG CAATTCGACA AAGTCTTTCC AGCCCTGGTA 
GCAGGCCTCC CCATTCACAT GTTCAGGACT GCGCACAATG CCCGGGAGAA ACTGGCAGAG 
AGCTTGAGGC ACGAGAACCT CCAAAAGAGG GAAAGCATCT CAGAACTGAT CAGCCTGCGC 
ATGTTTCTCA ATGACACTTT GTCCACCTTT GATGATCTGG AGAAGGCCAA GACACACCTC 
GTGGTCCTCT GGGCATCGCA AGCAAACACC ATTCCAGCGA CTTTCTGGAG TTTATTTCAA 
ATGATTAGGA ACCCAGAAGC AATGAAAGCA GCTACTGAAG AAGTGAAAAG AACATTAGAG 
AATGCTGGTC AAAAAGTCAG CTTGGAAGGC AATCCTATTT GTTTGAGTCA AGCAGAACTG 
AATGACCTGC CAGTATTAGA TAGTATAATC AAGGAATCGC TGAGGCTTTC CAGTGCCTCC 
CTCAACATCC GGACAGCTAA GGAGGATTTC ACTTTGCACC TTGAGGACGG TTCCTACAAC 
ATCCGAAAAG ATGACATCAT AGCTCTTTAC CCACAGTTAA TGCACTTAGA TCCAGAAATC 
TACCCAGACC CTTTGACTTT TAAATATGAT AGGTATCTTG ATGAAAACGG GAAGACAAAG 
ACTACCTTCT ATTGTAATGG ACTCAAGTTA AAGTATTACT ACATGCCCTT TGGATCGGGA 
GCTACAATAT GTCCTGGAAG ATTGTTCGCT ATCCACGAAA TCAAGCAATT TTTGATTCTG 
ATGCTTTCTT ATTTTGAATT GGAGCTTATA GAGGGCCAAG CTAAATGTCC ACCTTTGGAC 
CAGTCCCGGG CAGGCTTGGG CATTTTGCCG CCATTGAATG ATATTGAATT TAAATATAAA 
TTCAAGCATT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

REV-ERBa Regulates CYP7A1 through Repression of Liver Receptor Homolog-1.
Drug metabolism and disposition: the biological fate of chemicals(2017 Dec)
Zhang T,Zhao M,Lu D,Wang S,Yu F,Guo L,Wen S,Wu B

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CYP7A1 cDNA ORF clone, Homo sapiens(Human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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